<?xml version="1.0" encoding="UTF-8"?>
<uniprot xmlns="https://uniprot.org/uniprot"
 xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance"
 xsi:schemaLocation="http://uniprot.org/uniprot http://www.uniprot.org/docs/uniprot.xsd">
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BMC4</accession>
    <name>A0AAD5BMC4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Aminotransferase class I/classII large domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_011086</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011866">
        <property type="protein sequence ID" value="KAI7725759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BMC4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016847">
        <property type="term" value="F:1-aminocyclopropane-1-carboxylate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050478">
        <property type="entry name" value="Ethylene_sulfur-biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43795:SF39">
        <property type="entry name" value="AMINOTRANSFERASE CLASS I_CLASSII DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43795">
        <property type="entry name" value="BIFUNCTIONAL ASPARTATE AMINOTRANSFERASE AND GLUTAMATE/ASPARTATE-PREPHENATE AMINOTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00753">
        <property type="entry name" value="ACCSYNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="3">
        <location>
            <begin position="82"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7725759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="422" mass="47605" checksum="D0290DD7085ABC15" modified="2024-05-29" version="1">MAIEIVQPKPSVGLSKMAVSETHGEDSPYFAGWKAYDENPYHETNNPSGVIQMGLAENQVSNDTTLNDLNYFCFSFNISWSELTMAMASFMEEVRGGKAKFNPDRVVLTAGATAANELLTFILANPGDALLVPTPYYPGFDRDLRWRTGVQIVPIHCDSSNNFQITPKALEAAYDHATSMNIKVRGVLITNPSNPLGATIQRKVLEEILDFATRKNIHLVSDEIYSGSAFYADEFVSIAEVLESRNYKDSERCHIVYSLSKDLGLPGFRVGTVYSYNDQVVTTARRMSSFTLISSQTQFLLASMLSDKNFTRKYIKINRERLKKRYEMIINGLKKAGIECLKGNAGLFCWMNLSPYLEERTIKSELAIWKTIMHEVRLNISPGSSCHCSEPGWFRVCFANMSEQTLEVALSRIHEFIKQRKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-04-02" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BMU6</accession>
    <name>A0AAD5BMU6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_014232</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012062">
        <property type="protein sequence ID" value="KAI7725089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.34.10:FF:000002">
        <property type="entry name" value="Heat shock 70 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="HEAT SHOCK PROTEIN 70KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="266"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="312"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="269"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="288"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="326"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7725089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="336" mass="37753" checksum="ECDD13AD13571076" modified="2024-05-29" version="1">MGRSSIVLAIVLIGKVFRMFEDKEVQRDMKLFPYKIVNKDGKPYIQVKLKDGEIKVFSPEEISAMILTKMKETAEAFLGKKIKDAVVTVPDILLLDVAPLTLGIETVGGVMTKLIPRNTVIPTKKSQVFTTYQDQQTVVSIKVYEGERSLTKDCRLLGTFDLTGIPPAPRGTPQIEVTFEVDANGILNVKAEDKASGKSEKITITNEKGRLSQEEIERMVREAEEFAEEDKKVKEKIDARNALETYVYNMKNQISDKDKLADKLEEDEKEKIETATKEALEWLDDNQSAEKEEYDEKLKEVEAVXXXXXXXXXXXXXXXGGGAESTGDDDDEHDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BMU9</accession>
    <name>A0AAD5BMU9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">18S pre-ribosomal assembly protein gar2-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_014358</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011719">
        <property type="protein sequence ID" value="KAI7726302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BMU9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009786">
        <property type="term" value="P:regulation of asymmetric cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040378">
        <property type="entry name" value="BASL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33914">
        <property type="entry name" value="18S PRE-RIBOSOMAL ASSEMBLY PROTEIN GAR2-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33914:SF2">
        <property type="entry name" value="OS02G0582100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="214"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="214"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7726302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="372" mass="40817" checksum="501A12070BA15A17" modified="2024-05-29" version="1">MKESPGRYINPFLSDEENDKVNCITNLDDGMFEKSLEPSNVRSESLEKAKEVYIDKNVTCYEENGYNVVKDICVDEGLSNEEMVKCDKEQHELSCSPVVGDGDKHDDMIKDDIDTQLKSSMLEDCCKNSCLSSVTEDDNVPSSHASDKIELFVESNIRPDNSTQNGEEELDSSSNLLNDSIRVLSEDKLNSSSNLVNNSISVLEAADNSVNGVNQQLSEQQSTGSEPNEPRPTSHTFDIPNEIKTEEITNSDSDDVKPSTASGSHELLLNTESPPNHLDMASNNILAAPRGGGETSFSMAGSVSGHITFLGPITSAGSISHRSDGSNTSVRSFAFPILQNEWNSSPVRMAKADPRQSRKHRGWRQALLCCRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BNK2</accession>
    <name>A0AAD5BNK2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PHD-type zinc finger plants domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_018849</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011685">
        <property type="protein sequence ID" value="KAI7726389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BNK2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056874">
        <property type="entry name" value="PHD_dom_pln"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33779">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33779:SF11">
        <property type="entry name" value="OS04G0551600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25054">
        <property type="entry name" value="PHD_pln"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PHD-type zinc finger plants" evidence="2">
        <location>
            <begin position="10"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7726389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="120" mass="13677" checksum="1136CE853738BAEC" modified="2024-05-29" version="1">MMVDVERVCCMCGDVGFPDKIFRCITCHHRFQHSYCSNYFNESTDTPELCDWCQADEINKRTKHGGCSKKPAAGRRSEYSGDKIKLNDREEGSEKKKSPAGAPSPKTSTRRYKLLKDVMC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BSG9</accession>
    <name>A0AAD5BSG9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcription factor MYC/MYB N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_023817</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011132">
        <property type="protein sequence ID" value="KAI7728838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BSG9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025610">
        <property type="entry name" value="MYC/MYB_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46633">
        <property type="entry name" value="TRANSCRIPTION FACTOR MYC/MYB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46633:SF7">
        <property type="entry name" value="TRANSCRIPTION FACTOR MYC_MYB DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14215">
        <property type="entry name" value="bHLH-MYC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription factor MYC/MYB N-terminal" evidence="3">
        <location>
            <begin position="14"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7728838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="346" mass="38928" checksum="8BCF24CB7E5AD286" modified="2024-05-29" version="1">MEEQLNQSTITHLLQHTLRSFCIHENSQWVYAVFWRILPRNYPPPKWDTQGGAYDRSRGNRRNWILAWEDGFCNFAASTAGETSSGDSTAYANQEYQQYLGLQPELFFKMSHEIYNYGEGDSTGWSGLNNRLITVAKLKPTDQEINFLSTWNNSADSYPRTWEAQFQAGIKTIALVAVREGVIQLGSINKVTEDLSYVVMLRKKLSYIESIPGVLLPHPSSSLYPYKSEGYNTPETWPFQGGGDLPAVYTQPMNITPSMSSLEALLSKLPSVVPASSSQVEAPLYGEATQPRFVAVSPEKELVEGEDVEIKDVGECSSSMSSYDHHHQRFQHHGSNVSSCMTNNRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BSX5</accession>
    <name>A0AAD5BSX5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NAD-dependent epimerase/dehydratase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_019328</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011345">
        <property type="protein sequence ID" value="KAI7727579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BSX5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051207">
        <property type="entry name" value="ComplexI_NDUFA9_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12126:SF16">
        <property type="entry name" value="MIOREX COMPLEX COMPONENT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12126">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE 39 KDA SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="1">
        <location>
            <begin position="61"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7727579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="230" mass="24854" checksum="545176F3981D1A37" modified="2024-05-29" version="1">MSRLIHSRLLLSPSRLSNTLGSIGRGGRYFSTDSNNKIDEPLKVEEAETLPPPPPPVEKLLVLGGNGFVGSHICKEALDRGLLVASLSRSGKSSIQESWANKVNWHQGDLLSGDSWKEALDGVTSVPKRATETELLTKFPYGHVILRPGFIYGTRRVGSMKLPLGVIGSPLEMVLQYAKPLTQVPIVGPVLTPPVDVKAVAKVAVRGATDPVFPPGIVDVYGLKRYSQQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BU94</accession>
    <name>A0AAD5BU94_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_033666</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Secondary metabolite biosynthesis; flavonoid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the iron/ascorbate-dependent oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010926">
        <property type="protein sequence ID" value="KAI7729752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BU94"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031418">
        <property type="term" value="F:L-ascorbic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009813">
        <property type="term" value="P:flavonoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.330:FF:000016">
        <property type="entry name" value="Naringenin,2-oxoglutarate 3-dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050295">
        <property type="entry name" value="Plant_2OG-oxidoreductases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47991">
        <property type="entry name" value="OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0284">Flavonoid biosynthesis</keyword>
    <keyword evidence="7 9" id="KW-0408">Iron</keyword>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0847">Vitamin C</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="10">
        <location>
            <begin position="233"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7729752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="400" mass="45498" checksum="F1CAEDDB42D4C345" modified="2024-05-29" version="1">MSYNPHPRVVVTTLKPTNQNHLIATNTSHLNPTIHTHKHSHSFMAPVSLKWDETSLHENRFVRDEDERPKVPYNKFSNEIPVISLEGIDDVEGSRRVEICDKIVKACEDWGIFQVVDHGVDTKLLSEMTRLAREFFDLPDEEKLRFDMTGGKKGGFIVSSHLQGETVQDWREIVTYFSYPIKARDYSRWPDKPKEWRAVTEKYSEDLMGLACKLLEVLSEAMGLEKEALTKACVDMDQKVVVNYYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATRDGGKSWITVEPVEGAFVVNLGDHGHYLSNGRFKNADHQAVVNSNTSRLSIATFQNPAPDAIVYPLKVNEGEKSIMEKPITFMEMYKKKMSTDLELARLKKLAKEQQDTEKVKPIENIFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BW28</accession>
    <name>A0AAD5BW28_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_010262</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011006">
        <property type="protein sequence ID" value="KAI7729424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BW28"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000121">
        <property type="entry name" value="Myb transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015495">
        <property type="entry name" value="Myb_TF_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47999:SF95">
        <property type="entry name" value="HOMEODOMAIN-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47999">
        <property type="entry name" value="TRANSCRIPTION FACTOR MYB8-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="7">
        <location>
            <begin position="9"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="6">
        <location>
            <begin position="9"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="7">
        <location>
            <begin position="62"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="6">
        <location>
            <begin position="62"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7729424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="300" mass="33503" checksum="8EF566882D29969E" modified="2024-05-29" version="1">MGRAPCCEKVGLKRGRWTAEEDEKLNNYIKLHGEGSWRSLPKNAGLLRCGKSCRLRWINYLRSDLRRGNISKEEEQLITNLHASLGNRWSLIAAHLPGRTDNEIKNYWNSHLSRKILPSRRLMNPPAIAIAPTINNRKGRTSQSTMKINKANYRASTTIKDHKMVQSNGPPTKEHTKADLGDEDEMLCFIENIMEPDMLTAVEDGSSKGGSISEPMSDVISEGKEAIKSVTSEQMDDHVGGTSSSTTNSLDDWNWDFDFDVEDGVLGFGGEEYDNILSWPWESLAIDNDNFLGAGESGLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BW46</accession>
    <name>A0AAD5BW46_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_010721</name>
        <name evidence="5" type="ORF">M8C21_016361</name>
    </gene>
    <organism evidence="5 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010797">
        <property type="protein sequence ID" value="KAI7730334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAMZMK010008628">
        <property type="protein sequence ID" value="KAI7739378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BW46"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.110.10">
        <property type="entry name" value="Plant lipid-transfer and hydrophobic proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036312">
        <property type="entry name" value="Bifun_inhib/LTP/seed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016140">
        <property type="entry name" value="Bifunc_inhib/LTP/seed_store"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033872">
        <property type="entry name" value="nsLTP2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33214">
        <property type="entry name" value="BIFUNCTIONAL INHIBITOR/LIPID-TRANSFER PROTEIN/SEED STORAGE 2S ALBUMIN SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33214:SF85">
        <property type="entry name" value="BIFUNCTIONAL INHIBITOR_PLANT LIPID TRANSFER PROTEIN_SEED STORAGE HELICAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00234">
        <property type="entry name" value="Tryp_alpha_amyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47699">
        <property type="entry name" value="Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042441276" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein" evidence="3">
        <location>
            <begin position="29"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical" evidence="4">
        <location>
            <begin position="35"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7730334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7739378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="98" mass="11024" checksum="0B788FBF54B53F87" modified="2024-05-29" version="1">METSLRSCFTVVLMVLLLSAGHLQQAEAQSRCDSVQLSWCLQSIVSNMPPSQECCRRFRTQQACLCRETRDPTFGGYLRHPGARRVATACGVRFPRCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-04-02" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BX03</accession>
    <name>A0AAD5BX03_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Apoptosis inhibitor 5-like protein API5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_006819</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the API5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010579">
        <property type="protein sequence ID" value="KAI7730970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043067">
        <property type="term" value="P:regulation of programmed cell death"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008383">
        <property type="entry name" value="API5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12758:SF19">
        <property type="entry name" value="APOPTOSIS INHIBITOR 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12758">
        <property type="entry name" value="APOPTOSIS INHIBITOR 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05918">
        <property type="entry name" value="API5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="474"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="515"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="527"/>
            <end position="539"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7730970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="539" mass="60121" checksum="02FB8ED5280666AA" modified="2024-05-29" version="1">MSDAPNDANDIEKLYVYGATLSEAKDKSQNVDDYKSIIEAARSSSIKARQLAAQLIPRFFKFFPGLSGSAVDAHLDLCEAEELGIRVQAIRGLPLFCKDTPEHISKIVDILAQLLIAEENVERDAVHKALLSLLRQDVKASLTALFKHIESVDEPVTDDNLRERTLIFIRDKVFPLKSELLKPQEQMERHITDLIKKSLQDVTGAEFKMFMXXXXKAPTERVQELVEIIEGQADLDAQFSVEDGDHVDRLISCLYMALPFFIRGASSSKFLNYLNKHILPVFDKLPEERKVDLLKNLAESSPYAAPQDSRQILPSVVQLLKKYMPRRKTGEEMNFTYVECMLYTFHHLANKAPNATNSLCGYKIVTGQPSDRLGEDFSEFYKDFTERLSCVEDLTKATMKKLTQGMGQQNKTGTAISDEEKAKIKIQKQNATTGLRTCNNILAMTQPLHAKSPSFIGDKRINLSWNEAVKTVAPANTAGVKRPANAVKGPSNQPNKRGRGGSNQIVDRAFDSHGGRGGSRGGGQGRGRGRQGRGRGYYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BXE5</accession>
    <name>A0AAD5BXE5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Phospholipase D</fullName>
            <ecNumber evidence="4 11">3.1.4.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">M8C21_007643</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">AA19_3_7</strain>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Hydrolyzes glycerol-phospholipids at the terminal phosphodiesteric bond.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 17">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1,2-diacyl-sn-glycero-3-phosphate + choline + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14445"/>
            <dbReference type="ChEBI" id="CHEBI:15354"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="EC" id="3.1.4.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 11">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the phospholipase D family. C2-PLD subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.4" evidence="4 11"/>
    <dbReference type="EMBL" id="JAMZMK010010830">
        <property type="protein sequence ID" value="KAI7730191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BXE5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004630">
        <property type="term" value="F:phospholipase D activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046470">
        <property type="term" value="P:phosphatidylcholine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009395">
        <property type="term" value="P:phospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04015">
        <property type="entry name" value="C2_plant_PLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.870.10:FF:000027">
        <property type="entry name" value="Phospholipase D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.870.10">
        <property type="entry name" value="Endonuclease Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001736">
        <property type="entry name" value="PLipase_D/transphosphatidylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024632">
        <property type="entry name" value="PLipase_D_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015679">
        <property type="entry name" value="PLipase_D_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011402">
        <property type="entry name" value="PLipase_D_pln"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18896">
        <property type="entry name" value="PHOSPHOLIPASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18896:SF60">
        <property type="entry name" value="PHOSPHOLIPASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12357">
        <property type="entry name" value="PLD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00614">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036470">
        <property type="entry name" value="PLD_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00155">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56024">
        <property type="entry name" value="Phospholipase D/nuclease"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50035">
        <property type="entry name" value="PLD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0106">Calcium</keyword>
    <keyword evidence="7 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 11" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="C2" evidence="13">
        <location>
            <begin position="1"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="PLD phosphodiesterase" evidence="14">
        <location>
            <begin position="307"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="PLD phosphodiesterase" evidence="14">
        <location>
            <begin position="651"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="779"/>
            <end position="805"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR036470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAI7730191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="805" mass="91207" checksum="CAA2D4B8C06DCD57" modified="2024-05-29" version="1">MPSSCSDTDSIVYLHGDLDLTILEARCLPNMDMLSERMHRCITIFDTCKSPSLQRTKSQRRRKIITSDPYVTVSVGEATVARTRVISNSQNPIWNEHFVTPLAHPATEIEFQVKDNDVFGADLIGVVTISAERVISGEVIDGWFPIIGPSGKPPKPNAAIRLQVKFTPCDYRNTVAIGTDELFGLKESYFPVRHGGNVTLYQDAHVRDGEIPEIGLDEGKVFEQRGCWEDICHAILEAHHLVYIVGWSIYDKVKLVREPTKPMRNGWDLSLGELLKYKSQEGVRVLLLVWDDKTSHNKFFIKTVVGTLYTHHQKCVIVDTQAHGNNRKISAFIGGLDLCDGRYDTPKHRLFRDLDTVFANDFHNPTLPTGTKGPRQPWHDLHCKIDGPAAYDVLKNFEQRWRKATKWSEFGRRFKRISHWHDDSLIKLERISWILSPSSTLPNDDPALWVSKETDPENWHVQVFRSIDSGSLKGFPNDVQLAESQNLVCAKNLVIDRSIQKAYIQAIRSAKNFIYIENQYFLGSSYAWNSYKDAGADHLIPIELALKIASKIRANERFAVYIIIPMWPEGVPTSASVQEILFWQGQTMQMMYEVIARELKNSKLENAHPQDYLNFYCLGNREQCDEHVSNSGSHTSSNGGSVSASQKHGRFMIYVHAKGMIVDDEYVIIGSANINQRSMAGSRDTEIAMGAYQPHHTWAHKKAHPRGQVYGYRMSLWAEHTGKVEDYFQKAESLTCVENINKLAEENWQKFTDDDYTPLQGHLLKYPIKVDSDGKVTPLPGHEQFPDVGGKVLGSPKTLPSALTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BZ81</accession>
    <name>A0AAD5BZ81_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Homeobox protein knotted-1-like 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_012237</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TALE/KNOX homeobox family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010287">
        <property type="protein sequence ID" value="KAI7732202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BZ81"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000143">
        <property type="entry name" value="homeobox protein knotted-1-like 3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005539">
        <property type="entry name" value="ELK_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008422">
        <property type="entry name" value="KN_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005540">
        <property type="entry name" value="KNOX1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005541">
        <property type="entry name" value="KNOX2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050224">
        <property type="entry name" value="TALE_homeobox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11850">
        <property type="entry name" value="HOMEOBOX PROTEIN TRANSCRIPTION FACTORS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03789">
        <property type="entry name" value="ELK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05920">
        <property type="entry name" value="Homeobox_KN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03790">
        <property type="entry name" value="KNOX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03791">
        <property type="entry name" value="KNOX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01188">
        <property type="entry name" value="ELK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01255">
        <property type="entry name" value="KNOX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01256">
        <property type="entry name" value="KNOX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51213">
        <property type="entry name" value="ELK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 5" id="KW-0371">Homeobox</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ELK" evidence="9">
        <location>
            <begin position="199"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="8">
        <location>
            <begin position="219"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox; TALE-type" evidence="5">
        <location>
            <begin position="220"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="276"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="281"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="291"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7732202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="301" mass="33856" checksum="F7D9C901F431394D" modified="2024-05-29" version="1">MKPSLSHSHSQLNTTVAGDVPTTGDGGAVMNWQYASQKGAVLSHPLYDQLLAAHVACLRIATPVDQLPRIDAQLAQAQDIVAKYSAFGNVDPDDKELDHFMTHYVLLLCSFKEQLQQHVRVHAMEAVMACWEIEQSLQSLTGVSPGEGTGATMSDDDEDQVDSDVNLFDGSLDGQDSMGFGPLVPTESERSLMERVRQELKHDLKQGYKEKIVDIREEILRKRRAGKLPGDTTSVLKAWWQAHAKWPYPTEEDKARLVEETGLQLKQINNWFINQRKRNWHSNPSSSTALKTKRKRDTRQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BZT4</accession>
    <name>A0AAD5BZT4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">glycine--tRNA ligase</fullName>
            <ecNumber evidence="4">6.1.1.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Diadenosine tetraphosphate synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">M8C21_007950</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">AA19_3_7</strain>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>tRNA(Gly) + glycine + ATP = glycyl-tRNA(Gly) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:16013"/>
            <dbReference type="Rhea" id="RHEA-COMP:9664"/>
            <dbReference type="Rhea" id="RHEA-COMP:9683"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78522"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.14"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.14" evidence="4"/>
    <dbReference type="EMBL" id="JAMZMK010010408">
        <property type="protein sequence ID" value="KAI7731663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BZT4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004820">
        <property type="term" value="F:glycine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070150">
        <property type="term" value="P:mitochondrial glycyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00774">
        <property type="entry name" value="GlyRS-like_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00858">
        <property type="entry name" value="GlyRS_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.230:FF:000001">
        <property type="entry name" value="Glycine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.720.200:FF:000001">
        <property type="entry name" value="Glycine--tRNA ligase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.800:FF:000004">
        <property type="entry name" value="Glycine--tRNA ligase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000010">
        <property type="entry name" value="Glycyl-tRNA synthetase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.720.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.10">
        <property type="entry name" value="S15/NS1, RNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027031">
        <property type="entry name" value="Gly-tRNA_synthase/POLG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033731">
        <property type="entry name" value="GlyRS-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002315">
        <property type="entry name" value="tRNA-synt_gly"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009068">
        <property type="entry name" value="uS15_NS1_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000738">
        <property type="entry name" value="WHEP-TRS_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00389">
        <property type="entry name" value="glyS_dimeric"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003211">
        <property type="entry name" value="PRK04173.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10745:SF0">
        <property type="entry name" value="GLYCINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10745">
        <property type="entry name" value="GLYCYL-TRNA SYNTHETASE/DNA POLYMERASE SUBUNIT GAMMA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00458">
        <property type="entry name" value="WHEP-TRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01043">
        <property type="entry name" value="TRNASYNTHGLY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00991">
        <property type="entry name" value="WHEP-TRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47060">
        <property type="entry name" value="S15/NS1 RNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51185">
        <property type="entry name" value="WHEP_TRS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="WHEP-TRS" evidence="15">
        <location>
            <begin position="43"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="14">
        <location>
            <begin position="247"/>
            <end position="588"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAI7731663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="721" mass="80573" checksum="414766BC66533FE9" modified="2024-05-29" version="1">MRYYPTTTISSSLFSLFRRTRSLRSSAVVTPPMATTEDSLRQALAEKQSTIDARGTAIRQLKSSGASKSEIDEAVKALNDLKLEKTSIENQLKASISGEGSSCNKEAFRQAVVNTLERRLFYIPSFKIYRGVAGLYDYGPPGCAVKSNVLAFWRQHFVLEEDMLEVDCPCVTPEVVLKASGHVDKFTDLMVKDEKTGNCYRADHLLKDFCKDKLENDLAMSADKAAELKHVLAVLDDLSGEQLGAKIKEYGIVAPDTKNPLSDPYPFNLMFQTQIGPSGASTGYMRPETAQGIFVNFRDLYYYNGNKLPFAAAQIGQAFRNEISPRQGLLRVREFTLAEIEHFVDPDDKSHPKYSEVSSLKFLMFPRDEQMSGQSAKVLCLGDAVSKGIVNNQTLAYFIGRVYLFLTRLGIDEKRLRFRQHLPNEMAHYAADCWDAEIESSYGWIECVGIADRSAYDLRAHTDKSGVALVAQEKFAEPKEVEKLVITPVKKELGLAFKGSQKMVVEALEAMNEKEAMDMKAALETKGEVEFEVCTLGKTVTIKKNMLSISKEIKKEHQRVFTPSVIEPSFGIGRIIYCLYEHSFYMRPSKAGDEQLNVFRFPPLVAPIKCTVFPLVQNQQYEEVAKQISRSLTAAGVSNKKDITGTSIGKRYARTDELGVPFAVTVDSTSSVTIRERDSKDQIRVNVDEVAAVIKEVSEGRCQWTDILQKYPAHTSASADE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C242</accession>
    <name>A0AAD5C242_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ribulose-phosphate 3-epimerase</fullName>
            <ecNumber evidence="7">5.1.3.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Pentose-5-phosphate 3-epimerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_005322</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>D-ribulose 5-phosphate = D-xylulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:13677"/>
            <dbReference type="ChEBI" id="CHEBI:57737"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="EC" id="5.1.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ribulose-phosphate 3-epimerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.1" evidence="7"/>
    <dbReference type="EMBL" id="JAMZMK010009875">
        <property type="protein sequence ID" value="KAI7733936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C242"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004750">
        <property type="term" value="F:D-ribulose-phosphate 3-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006098">
        <property type="term" value="P:pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00429">
        <property type="entry name" value="RPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000181">
        <property type="entry name" value="Ribulose-phosphate 3-epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02227">
        <property type="entry name" value="RPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026019">
        <property type="entry name" value="Ribul_P_3_epim"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000056">
        <property type="entry name" value="Ribul_P_3_epim-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004076">
        <property type="entry name" value="PRK05581.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01163">
        <property type="entry name" value="rpe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11749">
        <property type="entry name" value="RIBULOSE-5-PHOSPHATE-3-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00834">
        <property type="entry name" value="Ribul_P_3_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01086">
        <property type="entry name" value="RIBUL_P_3_EPIMER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00009541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7733936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="267" mass="28966" checksum="B706ABF28DC1EC12" modified="2024-05-29" version="1" fragment="single">MRVVGKLMSANYRAFGRLSISTKSHHNNNTTPTLHCSPISDHKTLSELRKMASVEAKIAPSMLSSDFANLASEAHRMLTFGADWLHMDIMDGYVSLSLFHFVPNLTIGAPVIESLRKHTKAYLDCHLMVTNPLDYVDQLGKAGASGFTFHVEVSKDNWPELVKQIKSKGMRPGVALKPGTPVEEVFPLLEGENPVEMVLVMTVEPGFGGQKFMPDMMNKVRTLREKYPKLDIEVDGGLGPSTIDAAAAAGANCIVAGSSVFGAPEPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C3R4</accession>
    <name>A0AAD5C3R4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">M8C21_015087</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">AA19_3_7</strain>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010009975">
        <property type="protein sequence ID" value="KAI7733461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C3R4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000173">
        <property type="entry name" value="Probable serine/threonine-protein kinase At1g01540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000035">
        <property type="entry name" value="Putative receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052232">
        <property type="entry name" value="RLK_Ser/Thr-Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47984">
        <property type="entry name" value="OS01G0323000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47984:SF31">
        <property type="entry name" value="OS03G0227900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 14 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 15" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="27"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="18">
        <location>
            <begin position="165"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="120"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="443"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="126"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAI7733461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="470" mass="51783" checksum="076BF53D454E2D2F" modified="2024-05-29" version="1">MADSGNTPSSGGGVSLTSKTSILGQKLYVVIIVTIIIVTVLLFLIMYYIRRNSKAKRRRSSRMHVEHGSGQLPLVSEMINDESTKETIKLSAVDKDVNDDEKDVIKPMVKVVESKLGFTNSGSNESGTSRSDGASSSSVSAENLSNMGWGRWYSLNELEIATSNFATENVIGEGGYGIVYRGVVRDGTVVAVKYLQNNKGQAEKEFKVEVEAIGKVRHKNLVGLIGFCADGAKRLLVYEYVDNGNLEQWLHGDVGPISPLTWDIRMRIAIGTAKGLAYLHEGLEPKVIHRDVKSSNILLDRKWNAKVSDFGLAKLLGPEKSHVTTRVMGTFGYVSPDYASTGMLNECSDVYSFGVLLMEIVTGRSPVDYSREPGEMNLVDWFKGMVASRRGEELVDPKIKVQPPSRALKRVLLVCLRCIDMDANKRPKMGQIVHMLEADDFPFRTEPRPPRDMATFRAGEDVGSKAPSVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C4T4</accession>
    <name>A0AAD5C4T4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Plasma membrane ATPase</fullName>
            <ecNumber evidence="16">7.1.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">M8C21_011812</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">AA19_3_7</strain>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>ATP + H2O + H(+)(in) = ADP + phosphate + 2 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:20852"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.1.2.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 16">Cell membrane</location>
            <topology evidence="1 16">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIIA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.1.2.1" evidence="16"/>
    <dbReference type="EMBL" id="JAMZMK010009735">
        <property type="protein sequence ID" value="KAI7734408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C4T4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008553">
        <property type="term" value="F:P-type proton-exporting transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120029">
        <property type="term" value="P:proton export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02076">
        <property type="entry name" value="P-type_ATPase_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000045">
        <property type="entry name" value="ATPase 4 plasma membrane-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000004">
        <property type="entry name" value="Plasma membrane ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000005">
        <property type="entry name" value="Plasma membrane ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000211">
        <property type="entry name" value="Plasma membrane ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006534">
        <property type="entry name" value="P-type_ATPase_IIIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01647">
        <property type="entry name" value="ATPase-IIIA_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42861">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00120">
        <property type="entry name" value="HATPASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 16" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="13 16" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 16" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 16" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="16" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="551"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="579"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="616"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="659"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="690"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="723"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="17">
        <location>
            <begin position="18"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU362083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAI7734408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="855" mass="94064" checksum="3424E722265B0F63" modified="2024-05-29" version="1">MGGDTALSLEGIKNETVDLEKVPIEEVFEQLKCNREGLSSDEGAQRLEIFGPNKLEEKKESKILKFLGFMWNPLSWVMEAAAIMAIALANGGGRPPDWQDFVGIVCLLVINSTISFIEENNAGNAAAALMAGLAPKTKVLRDGRWSEQEAAILVPGDIISIKLGDIVPADARLLEGDPLKIDQSALTGESLPVTKNPYDEVFSGSTCKQGELEAVVIATGVHTFFGKAAHLVDSTNQVGHFQKVLTAIGNFCICSIAVGMAEQVLLYAARASRTENQDAIDAAIVGTLADPKEARAGIREVHFFPFNPVDKRTALTYIDNNGNWFRASKGAPEQILTLCGCKEDLKKKVHAMIDKFAERGLRSLGVARQEVPQKSKDSAGGPWEFVGLLSLFDPPRHDSAETIRRALNLGVNVKMITGDQLAIAKETGRRLGMGTNMYPSSSLLGGHKDESIAGLPVDELIEKADGFAGVFPEHKYEIVKKLQERKHICGMTGDGVNDAPALKKADIGIAVADATDAARSASDIVLTEPGLSVIISAVLTSRAIFQRMKNYTIYAVSITIRIVFGFMFIALIWKFDFSPFMVLIIAILNDGTIMTISKDRVKPSPLPDSWKLKEIFATGVALGGYLALMTVIFFWIMKDTDFFSDKFSVKSLRTSDPEMMAALYLQVSIVSQALIFVTRSRSWSFIERPGFLLMGAFLAAQLVATLIAVYAEWGFARIKGIGWKWAGVIWLYSIVFYFPLDIMKFAIRYILSGKAWLSMIDNRTAFTTKKDYGRGEREAQWAHAQRTLHGLQAPDTSNLFNEKSSYRELSEIAEQAKRRAEVARLREVLTLKGHVESVVKLKGLDIDTIQQHYTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C5S1</accession>
    <name>A0AAD5C5S1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ENTH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_015394</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Clathrin-coated vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009439">
        <property type="protein sequence ID" value="KAI7735737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C5S1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030136">
        <property type="term" value="C:clathrin-coated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005545">
        <property type="term" value="F:1-phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032050">
        <property type="term" value="F:clathrin heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048268">
        <property type="term" value="P:clathrin coat assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072583">
        <property type="term" value="P:clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006900">
        <property type="term" value="P:vesicle budding from membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011417">
        <property type="entry name" value="ANTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045192">
        <property type="entry name" value="AP180-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22951">
        <property type="entry name" value="CLATHRIN ASSEMBLY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22951:SF32">
        <property type="entry name" value="OS06G0175500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07651">
        <property type="entry name" value="ANTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50942">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="3" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENTH" evidence="5">
        <location>
            <begin position="27"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7735737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="64" mass="6969" checksum="831950CED36DD90C" modified="2024-05-29" version="1">MSGGGSQSSLRKALGAIKDSTTVNIAKVSSDYKELDINIVKATNHVERPAKEKHIRGEYIVIEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C6D1</accession>
    <name>A0AAD5C6D1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="9">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_004749</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the PPP phosphatase family. PP-2A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="9"/>
    <dbReference type="EMBL" id="JAMZMK010009474">
        <property type="protein sequence ID" value="KAI7735560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C6D1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07415">
        <property type="entry name" value="MPP_PP2A_PP4_PP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000003">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047129">
        <property type="entry name" value="PPA2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45619">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE PP2A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0464">Manganese</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="10">
        <location>
            <begin position="114"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7735560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="309" mass="35394" checksum="9254A411E021C28C" modified="2024-05-29" version="1">MSLDHVNHNVDEQITQLMECKPLSEQEVRALCDKAKEILMQESNVQPVKSPVTICGDIHGQFHDLAELFRIGGKCPDTNYLFMGDYVDRGYYSVETVTLLVALKVRYPQRITILRGNHESRQITQVYGFYDECLRKYGSANVWKTFTDLFDYFPLTALVESEIFCLHGGLSPSIETLDNIRNFDRVQEVPHEGPMCDLLWSDPDDRCGWGISPRGAGYTFGQDISEQFNHTNSLKLIARAHQLVMDGYNWGHEQKVVTIFSAPNYCYRCGNMASILEVDDCKGHTFIQFEPAPRRGEPDVTRRTPDYFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C6H5</accession>
    <name>A0AAD5C6H5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_022409</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009398">
        <property type="protein sequence ID" value="KAI7735997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C6H5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7735997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="87" mass="9973" checksum="B9FAFD6E84D8B102" modified="2024-05-29" version="1">MDIIIEDVRIRRLGLQLKAKDANPDTNWEKIINSIKKELDAKKKQLKPPLNSDVVNSGPDSCIPLSWMEIDKDFEDIESRGYIDCSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C7M2</accession>
    <name>A0AAD5C7M2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">Histone deacetylase</fullName>
            <ecNumber evidence="3 12">3.5.1.98</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">M8C21_032904</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">AA19_3_7</strain>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N(6)-acetyl-L-lysyl-[histone] + H2O = L-lysyl-[histone] + acetate</text>
            <dbReference type="Rhea" id="RHEA:58196"/>
            <dbReference type="Rhea" id="RHEA-COMP:9845"/>
            <dbReference type="Rhea" id="RHEA-COMP:11338"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="3.5.1.98"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11 12">Belongs to the histone deacetylase family. HD Type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.98" evidence="3 12"/>
    <dbReference type="EMBL" id="JAMZMK010009386">
        <property type="protein sequence ID" value="KAI7736043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C7M2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000118">
        <property type="term" value="C:histone deacetylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141221">
        <property type="term" value="F:histone deacetylase activity, hydrolytic mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040029">
        <property type="term" value="P:epigenetic regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09991">
        <property type="entry name" value="HDAC_classI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.800.20:FF:000001">
        <property type="entry name" value="Histone deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="entry name" value="Histone deacetylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003084">
        <property type="entry name" value="HDAC_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="HDACs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625:SF10">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037913">
        <property type="entry name" value="His_deacetylse_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01271">
        <property type="entry name" value="HISDACETLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="8 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="7 12" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Histone deacetylase" evidence="17">
        <location>
            <begin position="4"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="329"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="344"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="13">
        <location>
            <position position="93"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="216"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="255"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00061569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR037913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR037913-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR037913-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR037913-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAI7736043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="463" mass="52252" checksum="71F8F189F4EBCB96" modified="2024-05-29" version="1">MEIVRPFPANPDDITKFHSDDYVDFLASVTPDTVQDHTHIRHLKRFNVGEDCPVFDGIFDFCQASAGGSIGAAVKLNRQDADIAINWAGGLHHAKKSEASGFCYVNDIVLGILELLKVHRRVLYVDIDIHHGDGVEEAFFTTDRVMTVSFHKFGDFFPGTGHIKDIGANQGKYYALNVPLNDGMDDDSFRGLFRPIVHKVMEVYQPDAVVLQCGADSLAGDRLGCFNLSVKGHADCLRYLRSFNVPLMVLGGGGYTIRNVARCWCYETAVAVGVEPENKLPYNEYYEYFGPDYTLHVEPSPLDNQNTPKDLEKIRNMLLEQLSRLPHSPSVPFQVTPPVTEVPEEAKEPMEKRPKPRIWSGQEYDSDVDEEEKPRHQSFNANNSSTRHALRCSGVRKVKMGLCGMKDEDMMDGDAHSGVVTLLFCAAKNYEVVAFRRVMPKVVTLVNLWFNFLRMLTWMNYNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C7W0</accession>
    <name>A0AAD5C7W0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_027244</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily. AB hydrolase 4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009151">
        <property type="protein sequence ID" value="KAI7736968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C7W0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047372">
        <property type="term" value="F:monoacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034338">
        <property type="term" value="F:short-chain carboxylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000140">
        <property type="entry name" value="Esterase/lipase/thioesterase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050960">
        <property type="entry name" value="AB_hydrolase_4_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012020">
        <property type="entry name" value="ABHD4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10794">
        <property type="entry name" value="ABHYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10794:SF85">
        <property type="entry name" value="ALPHA_BETA HYDROLASE FOLD PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005211">
        <property type="entry name" value="Ab_hydro_YheT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="3">
        <location>
            <begin position="102"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="2">
        <location>
            <position position="182"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="2">
        <location>
            <position position="318"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="2">
        <location>
            <position position="347"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR005211-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7736968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="383" mass="42561" checksum="AE7B5D12487776FD" modified="2024-05-29" version="1" fragment="single">MATPVSNTRTTSNHFTDHHPSLEVLGGAHDSFCPAINTHLNRPYNHYPVLATNHHIETIFAAFFRTIPDVKLKRECLRTQDDGTVTLDWVSGDSRNLPENSPILLLLPGLTGGSDDSYVRHMLVRARNKGWRVVVFNSRGCADSPVTTPKMYSASFLGDLDEVVNHVVNRYPNANLYGVGWSMGANILVQYLGKEPGASRFSGAVSMCNPFDLVMSEEDLCKGFNKIYNRSLAKALSSILKKHLMLFEDIGGDYNIPLAANAKIIRDYDEGLTRVSFGFKSVDEYLQNSCSANYIKHVRTPLLCIQAILFTMNLAKNDPIAPDRAIPREDIKENPNCLLIVTPKGGHLGWVAGENAPVGCPWTDPMVMDFLRHLENEKSSTQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C886</accession>
    <name>A0AAD5C886_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_017612</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009219">
        <property type="protein sequence ID" value="KAI7736679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C886"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31245">
        <property type="entry name" value="UBIQUITIN SYSTEM COMPONENT CUE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31245:SF1">
        <property type="entry name" value="UBIQUITIN SYSTEM COMPONENT CUE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="122"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7736679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="213" mass="23971" checksum="AA339F943741BB92" modified="2024-05-29" version="1">MDKLVIERTLNQCGDDIETTIKSLTELRLVSNNNAGPAVGEGSEYQIQTQSVLDVAANGVANGEAGPFENSPHQKPVDGADWVDLFVKEMLSASNIDDARTRASRALEMLEKSIHERVTAEAHGLKQENMMMKEQLQALIQENSLLKRAVAIQHERQKEFEDRGQELHNLKQMVSQYQEQLRTLEVNNYSLTMHLKQAQQGSSMPGRFHPDVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C895</accession>
    <name>A0AAD5C895_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_020609</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in peroxisomal proliferation. Promotes peroxisomal duplication, aggregation or elongation without fission.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homooligomer. Interacts with ARC5 and FIS1B on peroxisomes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Peroxisome membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peroxin-11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009252">
        <property type="protein sequence ID" value="KAI7736535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016559">
        <property type="term" value="P:peroxisome fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044375">
        <property type="term" value="P:regulation of peroxisome size"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008733">
        <property type="entry name" value="PEX11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12652">
        <property type="entry name" value="PEROXISOMAL BIOGENESIS FACTOR 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12652:SF10">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 11C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05648">
        <property type="entry name" value="PEX11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="5" id="KW-0962">Peroxisome biogenesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7736535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="245" mass="26944" checksum="455BEE24AB643B91" modified="2024-05-29" version="1">MSTLDVARTELALAVLYLNKAEARDKICRAIQYGSKFVSNGEPGTAQNVDKSTSLARKVFRLFKFVNDLHALISPVAPGTPLPLVLLGKSKNALLSTFLFLDQIVWLHRSGIYKNKERAEIIGKVSLFCWMGSSICTTLVEIGEIGRLGKSMKKLDKELKGTDKYKNEQYRAKLKKSNERSLALIKSAMDIVVAVGLLQLAPKKVTPRVTGAFGFTTXXXSVTSVSTQVKSTLKQMILVATQPSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C8Y3</accession>
    <name>A0AAD5C8Y3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_009045</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009134">
        <property type="protein sequence ID" value="KAI7737054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33477">
        <property type="entry name" value="P-LOOP NTPASE DOMAIN-CONTAINING PROTEIN LPA1 HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33477:SF3">
        <property type="entry name" value="P-LOOP NTPASE DOMAIN-CONTAINING PROTEIN LPA1 HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13671">
        <property type="entry name" value="AAA_33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="224"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="447"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="229"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="461"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7737054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="667" mass="74009" checksum="3A5C75E566CE18B2" modified="2024-05-29" version="1">MEVGKLLYIVVEDDDNIGGHVNEELCFRYTRHVLQSTLQLMGCKARHAFKISQRVFEIMRTECLGDTLAAMSVIKTQGGGSSNTVLNNDDDKLSSSIPFESYKKRTTVIVKRRRFVDVVCDALAEYKYVGPNQRADLVLACRIRERKESMTVLLCGTSGCGKSTLSALLASRLGITTVVSTDSIRHMMRSFVDEKQNPLLWSSTYHAGEHLDVVAVSEAKAKRKAKKNGSVSPQSQFKNAPFDASAPEGLTTVDLISPKQMAIEGFKAQSEMVIDSLDRLITAWEERKESVVVEGVHLSLNFVMGLMKKHPSIIPFMIYITNEDKHMERFAVRAKREAGEQLCDSTTNTVAVVNEEYRNQCIANSLSSKGMFQLIQRQGSSRNLMALLNDDGSVAKVWPVHSVDADGKPIIGNPDYGPLQIGKAEPVNLQFGNFGISAWPTDVGCTSQAGSVDESRGELTDNGSRYHSSGCSSPKASDGPSKELKEEQSVYGSDEEGDDNXXXXXXXXXXXXXXXXXXXXXXXXXXXXVIDNVVYILFEQMEGSVDEESTKSDEEYDDLAMQDIQENGYLNEDEFDQKHNLIPPTQHDTFANYDVDKYSQNLDRFLRAKSEPHWDSVQPNPSIIKAEKMVVSSGSLIVKRRSHSVSCFGKDVSLSLTHDDGYCYTIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C962</accession>
    <name>A0AAD5C962_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_022701</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the AB hydrolase superfamily. Epoxide hydrolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009328">
        <property type="protein sequence ID" value="KAI7736251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C962"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000639">
        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43329">
        <property type="entry name" value="EPOXIDE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="3">
        <location>
            <begin position="27"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7736251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="312" mass="35257" checksum="265AF80E0576CB12" modified="2024-05-29" version="1">MDQIVQKFVKVNGVKIHLAEIGKESSPPVLFVHGFPEIWYSWRHQMIAVAGAGFRAISLDCRGYGLSDPPPNLDTFGFNDIVNDIHAIIDSLAIDKIFIIAKDAGVRPAYVFTLRYPTRVAGVVGLGIPHTPFGPPKFLTEMPEGFYVNRWREPGRAEADFGRLDAKTVVRNIYILFSRSEIPIASENQEIMDIVDASTALPSWFTEEDLSAYGALYEKSGFHNSLKVPYRTIEEEHDIEDPVIKNPMLLIIGEKDYFLKFPGVEDFIKSGMVKHFASDLEIEYLPEGSHFVQEQFPEKVNPLIIAFLRKHC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CAE8</accession>
    <name>A0AAD5CAE8_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_027536</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008826">
        <property type="protein sequence ID" value="KAI7738333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CAE8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010903">
        <property type="entry name" value="DUF1517"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053023">
        <property type="entry name" value="FLAP_modulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33975">
        <property type="entry name" value="MYELIN-ASSOCIATED OLIGODENDROCYTE BASIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33975:SF2">
        <property type="entry name" value="MYELIN-ASSOCIATED OLIGODENDROCYTE BASIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07466">
        <property type="entry name" value="DUF1517"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037221">
        <property type="entry name" value="DUF1517"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="109"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7738333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="316" mass="35201" checksum="B593967949E9A463" modified="2024-05-29" version="1">MALTPFMLECNYNSSHLLLQSSYLRPLRSLLGLLFLYDHHRSLVAMAASGGSMGGSDFSSSDDHSYNDYNYSCSGPTYYSSSGGYSRRTNSRAPKKEANEVEPMRLQEAVGVIVIVLLFLLIFVYAPGRDSAKTSVVKLQVGSLATGRSLQRDLNRIAEVADTSTNKGWHFVLQESILSLLRHSHDCISGYSSVDVKRSVVDCEKRFNQISIEERGKFDEQTLVNVNNIRKKSATSQSSNWLPNEYIVVTIIVAARGVPELPPIKSSEQLKNALQKMASIPSSNIMGVQVLWTPQKEDDSLTEQKMLKDYPMLHPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CCQ6</accession>
    <name>A0AAD5CCQ6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_007334</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008692">
        <property type="protein sequence ID" value="KAI7738945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CCQ6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35746">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT (PPR) SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35746:SF1">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT (PPR) SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="91"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="132"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="200"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="406"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="549"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="698"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="820"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="133"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="220"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="271"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="406"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="552"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="729"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="739"/>
            <end position="752"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7738945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="864" mass="94630" checksum="6E5485B2315E1678" modified="2024-05-29" version="1">MIKTRKRHTTNFNTFCFVGSCSITSSHTTPHHTIFFYKDFTRIFFLPSSSSSPKSFFFNPFFFCFLIFTQDMDARDQITHTTSSGHGVHLCRRCGWPFPNPHPSAKHRRAHKKICGTIDGYTNLLIGSEVVSDDDKEKTPSPKIEKKNGDGGSGGLRVSFSRSEDELFSDAVTEFADSGSASKHLDRDLFFSFKDAENDGTNELLNDPIESSKVDTSVEVSEKIDTHVEKSEETEKASVSASSESNRELTETGLLKEGVTGHSPEPGHISHSQETKSIDVAEAGEEKGQASQTSETITREDKNLELTKASPEVSAIVGGDHDIVKQGDFDKVEPAIADGINEDIKQEIKSDAEHVSEVVNNYKTAEETTIEKFGLEYEISPEIVKQSEIDHLEKSEEIISQQIVKEAEESMHEKSESDHKHAPEAAKEPEKENFGAPNVPIQEVFEEPVAVLTEKEDLEAHELEKSSIEQIQEVVKEPNTVLTEKEDIAGSNLEKQLNEHTHEVFENPISVLTEKQDLGAPELEKNSKEQIQEVVKEPDTVLTAKVDLSGPHLEEQSNKHNHEVVGKPDSVLTKSQDMGAPELEKCSKEHVEEPNLVLSKKEDLGAPKSEKCSKEEAIEQCVDNGSSVVVAANIDNSVVNSKNAADVVHVPIIEEARITNQDSGAALSVVSSGRSSLEGNWGSVSVLSTASIDAGTLQSTEKSKVNAGKSNAATSDVFEPPSFMTLVETESKDKKPESSEVQDSQKQPNPEDSQAGWFPTMAKVNNDSEGRKRNEEAIAKVTNWSTGKQAMPLKNLLGEAKSPSGAKPPPVVQKDEAIVNPPVNDDVASPPKLIDEDGKKGRKKVRGISSWVPFTCCSSVNVVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CDT5</accession>
    <name>A0AAD5CDT5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pentacotripeptide-repeat region of PRORP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_031107</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008490">
        <property type="protein sequence ID" value="KAI7740193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CDT5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:001209">
        <property type="entry name" value="Os07g0213300 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:003312">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47447">
        <property type="entry name" value="OS03G0856100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47447:SF21">
        <property type="entry name" value="PENTACOTRIPEPTIDE-REPEAT REGION OF PRORP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13812">
        <property type="entry name" value="PPR_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="14"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="10"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="45"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="80"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="115"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="150"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="187"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="222"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="257"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="292"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="362"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="397"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="432"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="467"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="607"/>
            <end position="641"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7740193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="710" mass="79357" checksum="9FDF1C23A98685B0" modified="2024-05-29" version="1">MKNQKNYCANNDIYNTMIRLYARHNQTDQARGLFFEMQKWRCKPDAETYNALISAHGRAGQWRWATNIMEDMLRAAVPPTRSTYNNLIHACGSSGNWKEALKVSKKMTENGVGPDLVTHNIVLSAFKTGSQYSKALSYFELMKGTKIRPDTTTLNIVIHCLGKLGHFEKAIDIFHSMRKKRAQCDPDIVTFTSIVHLYSVAGQIENCKAVFDTMIAEGFKPNIISYNALLGAYASRGMDKEALSVFNDIKKNGFRPDVVSYTSLINAYGKSQQPEKALEVFNKMKKNKLKPNLVTYNAMIDAFGSNGFLPEAVNMLHEMETNGVQPNIVTISTLLACCGRYGQKVKIDSILAAADSRGIRLNTVAYNSAIGSYMNIGEYDKAVALYKSMREKNVTPDSVTFNVLISGCSKMSKYTEALEYFNEMVDLNLTMSSEVYSSVISVYSKQGQLLEAESLFSKMRMTHGGPDVVAYTAMLHAYSTAEKWEDAFAVFQEMEMKGVEPDLIACSALMRAFNRGNQPAKVLVVAEFMQDRNIPMNETIYYEMLSACSILQDWRKTVELVGLMEPSFNTMSIGLLNQLLHLIGKSGKTETMIKVFYKIVATGAEINNSTYSVLLKNLLAAQNWRKYLEVMEWMEDSGVAPSVDMYQSILAFAQSSGREYSVNACEEKHAYKVSIDLSCTVSTMPAGKLESWSFGMVEGSSLNLCLLSEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CE46</accession>
    <name>A0AAD5CE46_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_010743</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the major facilitator superfamily. Phosphate:H(+) symporter (TC 2.A.1.9) family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008628">
        <property type="protein sequence ID" value="KAI7739400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CE46"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051119">
        <property type="term" value="F:sugar transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17358">
        <property type="entry name" value="MFS_GLUT6_8_Class3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000043">
        <property type="entry name" value="sugar transporter ERD6-like 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044775">
        <property type="entry name" value="MFS_ERD6/Tret1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050549">
        <property type="entry name" value="MFS_Trehalose_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48021">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48021:SF1">
        <property type="entry name" value="GH07001P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="52"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="90"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="122"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="152"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="181"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="205"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="286"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="326"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="353"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="394"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="427"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="459"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="12">
        <location>
            <begin position="57"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7739400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="493" mass="53241" checksum="53E71495A413C77D" modified="2024-05-29" version="1">MTSRDPEIEEGGGGSSLKKPLLHTGSWYRMAGMSSQQSNMSASSPQMMRESVSIFLCVSIVALGPIQFGFTCGYSNPTQADIITDLKLKISEFSVFGSLANVGAMVGAIASGQIAENIGRKGSLMIAAIPNILGWLAISFARDYSFLCMGRLLEGFGVGVISYTVPVYIAEISPQNLRGSLGSVNQLSVTIGIMLAYLLGLFLPWRVLAVVGTLPCIILIPGLFFIPESPRWLAKMGFMEDCETSLQVLRGFDTDISTELNEIKRSVATSGKRDTIRFSELKRRRFWYPLMVGIGLLLLQQLSGINGVLFYSSHIFKSAGISSSKAATFGLGAIQVVATAITTSVVDKSGRRALLLLSSFLMVASSLLASMSFFSKHLVSKDSSLYDFLGTLALIGLVIMVSGFSLGLGPIPWVIMSEILPVNIKSLAGSVATLFNWLAASIVTMTAPLLLHWSSGGTFLIYAAVSTFTLFFVKVWVPETKGKTLEEIQWSFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CEG2</accession>
    <name>A0AAD5CEG2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_012733</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008445">
        <property type="protein sequence ID" value="KAI7740386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CEG2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000148">
        <property type="term" value="C:1,3-beta-D-glucan synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003843">
        <property type="term" value="F:1,3-beta-D-glucan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006075">
        <property type="term" value="P:(1-&gt;3)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058851">
        <property type="entry name" value="CALS1_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003440">
        <property type="entry name" value="Glyco_trans_48_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12741:SF67">
        <property type="entry name" value="CALLOSE SYNTHASE 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12741">
        <property type="entry name" value="LYST-INTERACTING PROTEIN LIP5 DOPAMINE RESPONSIVE PROTEIN DRG-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25968">
        <property type="entry name" value="CALS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02364">
        <property type="entry name" value="Glucan_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Callose synthase helical" evidence="2">
        <location>
            <begin position="81"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase 48" evidence="1">
        <location>
            <begin position="181"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7740386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="412" mass="45882" checksum="AEDF480E23B70616" modified="2024-05-29" version="1" fragment="single">FSSSPGSSFAIGEATPPVVATSLSLFLSFSSSPGSSFAIGEATPPVVATVAGPLGWSATFNSSSKGRGWGNGLAFYAKQYRKVERIFREINHSIEENSLVMTLNLKKLPLVLSRFTALTGLLIMDETPQLAKGAAKAVYELYDVVTHNLLSSELSYISLSQHITSDSLASEGAVKDSANNIPKNLEARRRLEFFTNSLFMHMPAAKPNSVFTPYYSETVLYSSHDLWQENEDGISTIFYLQKIFPDEWENFLERIGRSNTGDLELQDSSTDALELRFWVSYRGQTLARTVRGMMYYRRALMLQSYLENRSLGGTPPLLERNPQASLSPQGFEQSREARAQADIKFTYVVSCQIYGQQKQRKEKEAADIALLLQRNEALRVAFIHVEESSGTDGKLVKSFYSKLVKADIHGKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CEI5</accession>
    <name>A0AAD5CEI5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_005278</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008505">
        <property type="protein sequence ID" value="KAI7740137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CEI5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35320">
        <property type="entry name" value="ATP-DEPENDENT CLP PROTEASE ATP-BINDING SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35320:SF1">
        <property type="entry name" value="ATP-DEPENDENT CLP PROTEASE ATP-BINDING SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7740137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="253" mass="27949" checksum="08A7E4B55EC73F28" modified="2024-05-29" version="1">MVYQIHSTHPQILLKIPINKPKLSNIHTKPQLQTRTTITSSSSPSISISTVNQENNPKQDIYSVKFKTLGACKLGISRYPDFEYNAQGGEGSGIGKKMVGVDFNGETSVDFDVKTLYIPPLTTATTKFLGLPLPPFLKIAIVPEIFRGIINLESDKVDLKFKAKFWFSMGSIYKAPPLLVETLLTSEESKGSLRSGIGERLDEQGRCKLVGVATVEPIDDFLMNSFLDLPTECLAILNATITFSKDEDFKLNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-10-08" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CEM9</accession>
    <name>A0AAD5CEM9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_013766</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008675">
        <property type="protein sequence ID" value="KAI7739101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019199">
        <property type="term" value="F:transmembrane receptor protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044812">
        <property type="entry name" value="CERK1/LYK3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46204">
        <property type="entry name" value="CHITIN ELICITOR RECEPTOR KINASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46204:SF27">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7739101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="112" mass="12534" checksum="28091656C7B8EAEC" modified="2024-05-29" version="1" fragment="single">VRLVGYXXXXXXXXXXYEYIENGNLSQHLHGSEREPLPWPARVQIALDSARGIEYIHEHTIPVGHVHCDIKSENILIDKNLRGKVADFGLTRLTAVGSKSRLAGTFGYMPPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CF71</accession>
    <name>A0AAD5CF71_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box associated beta-propeller type 3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_009427</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008454">
        <property type="protein sequence ID" value="KAI7740354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CF71"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09917">
        <property type="entry name" value="F-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055290">
        <property type="entry name" value="At3g26010-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013187">
        <property type="entry name" value="F-box-assoc_dom_typ3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017451">
        <property type="entry name" value="F-box-assoc_interact_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01640">
        <property type="entry name" value="F_box_assoc_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35546:SF16">
        <property type="entry name" value="F-BOX ASSOCIATED UBIQUITINATION EFFECTOR FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35546">
        <property type="entry name" value="F-BOX PROTEIN INTERACTION DOMAIN PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08268">
        <property type="entry name" value="FBA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box associated beta-propeller type 3" evidence="1">
        <location>
            <begin position="96"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7740354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="350" mass="40667" checksum="A85F194EC4377615" modified="2024-05-29" version="1">MDSKLPSEMIMEIMSRSSLKTLGIMRCASKELNKLAYEPYLLDLYKKRNKIVSGFIIQYMKRGWMNIKEFAPSPESNNLDLGFLPYNAQVLATSEQGIMVFKIQDPNNCRKYLYHVCKPATKQVLALPSPKGNNTDIKFAIVVMGLKPLHYKIIRFSMRRALRRWRYLHMAYRCDIFDSMTWKWRSLKPITLGDGVFLTNPQPVTKSGSIYMLLTNNNILKFDVFSEKWEVFSSPIPYDESRTSTELVKYGGRLGLACKPPNANGYWDIWVYKMDKSWEKDVDVAAESESERESLKALYDSDTSVMVNRETLLFYRLKQQGNSMISKIVLNDIPYQIFSFRSDFEPSDLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CFF7</accession>
    <name>A0AAD5CFF7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_014628</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008375">
        <property type="protein sequence ID" value="KAI7740663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CFF7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020428">
        <property type="entry name" value="PFA-DSPs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004861">
        <property type="entry name" value="Siw14-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03162">
        <property type="entry name" value="Y_phosphatase2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01911">
        <property type="entry name" value="PFDSPHPHTASE"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7740663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="130" mass="14722" checksum="26D30939EDCF25C7" modified="2024-05-29" version="1">MISMVYVDNEKPCAASVGDQSSRQANPSVVGAMAYVLIDDYHAVLYRYLCPESYPERNVEFLKANRIHLFQFGIEGTMEPFVNIPEITIRQTLQVFLAIIVPEKVWHPERWVGYEQEVLTLKGAASADVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CGR2</accession>
    <name>A0AAD5CGR2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_012907</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008221">
        <property type="protein sequence ID" value="KAI7741307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CGR2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35770">
        <property type="entry name" value="U2 SMALL NUCLEAR RIBONUCLEOPROTEIN AUXILIARY FACTOR-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35770:SF1">
        <property type="entry name" value="U2 SMALL NUCLEAR RIBONUCLEOPROTEIN AUXILIARY FACTOR-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="185"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="201"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7741307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="225" mass="24729" checksum="8AEEB0B651278ABA" modified="2024-05-29" version="1">MDLSKFEALFGEPNVESTVHTPFLFQVHAPDPCHLRVVVTDFLSTTFEAIRSIQQLDDMRDETGVGGSWSDFLEYLVNSLNFGDVKLVFDGQSKSAGPGSARLVAQKSKGMPRISVSLRSLLGTAANEAIASFLSSVEQGTNYYLAEKSEHLQKQLDGLVYSNRHTSQKIHDKLTSDTSAAATLYGISDKQSDQNPSPMKAPKRVVPAHRRSKVRGVLLQDTEDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CGZ1</accession>
    <name>A0AAD5CGZ1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">M8C21_019870</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="25">AA19_3_7</strain>
            <tissue evidence="25">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RLP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="25">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010008163">
        <property type="protein sequence ID" value="KAI7741593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009791">
        <property type="term" value="P:post-embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000309">
        <property type="entry name" value="Leucine-rich repeat receptor protein kinase MSP1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000479">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000233">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase TDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000041">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000111">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000445">
        <property type="entry name" value="MDIS1-interacting receptor like kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.310">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045381">
        <property type="entry name" value="BRI1_island_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051420">
        <property type="entry name" value="Ser_Thr_Kinases_DiverseReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48005">
        <property type="entry name" value="LEUCINE RICH REPEAT KINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48005:SF16">
        <property type="entry name" value="MDIS1-INTERACTING RECEPTOR LIKE KINASE 2-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20141">
        <property type="entry name" value="Island"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="16 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="12 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-0675">Receptor</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10 23" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 22" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="23">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042199369" description="non-specific serine/threonine protein kinase" evidence="23">
        <location>
            <begin position="27"/>
            <end position="1640"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="665"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="1307"/>
            <end position="1329"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="24">
        <location>
            <begin position="731"/>
            <end position="1005"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="24">
        <location>
            <begin position="1373"/>
            <end position="1640"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="759"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="KAI7741593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="1640" mass="181309" checksum="B907D787AB9140D2" modified="2024-05-29" version="1">MMAKTLYFNHLLLFLTLLQCLLVVNGGSGDFQQLLLFKSSLKNPNVLQNWIAGNNACNFTGVSCTNAKVSAINLTGIDLNCNFSSISSTLLVVPTLESFVAKNCNVSGNVVMTSHCNNVLKVLDMSGNRISGSVMDMRSLSSCLNLKTLILSRNFIEATDLTKPLALTLEVIDLSFNLISGPEFVPWMLSDGCGELVVFNVSSNNISGTVPLSLKSCSLLEVVDFSMNNLSGKLPMDIFLDLRRLKTLNLGFNNFIGRLPESLSDMTNLERFDVSSNRISGGIPGGICQGGDATRLHVLYLQNNRLTGSIPATVSNCSQLVSLDLSFNYLTGKIPESFGYLSKLQDLIIWMNLLTGEIPEELIYVQTLENLILDFNYLTGSIPASLSNCTNLNWISLSNNKLFGKIPAALGALSNLVFLRLGNNSFSGQIPAELGDCKSLVWLDLNTNQLSGTIPPALFKQSGFVASAYLTGLQHLYIKNDGSRQCHGAGNLLEFGGIRRQDLDRLSSRILCNYSRVYLGITQPNFNHNGSMIFLDLSYNKLDGGIPKELGSMYYLIILNLGHNNLTGPIPEELGGLKSTAILDFSHNRLNGSIPNSLTSLGLGDADLSYNNLSGPIPISAPFDTFPADRFSNNSGLCGYPLPKCRSGPKHKSNDQRENKRKNGLVHHLTIFLPIIGGLCFLFLVFMCYKIRKADKKNIQPETTRHGNVCSVLNYDGRIAYEDFITATEDFDLKYCIGTGGYGSVYKAKLPEGKTFALKKLHRFEAEQFNKSFMNEVQVLTNLRHKNIVKLYGFCLHNKCNFLVYEYIERGSLFCALSNSDLAVEVDWLKRVNIIKNVAHALSYMHHDCSPPIVHRDISTNNILLNSEMQGFVADFGVARLLDPYSSNQTTIAGTLGYIAPELAYNMIVTEKCDVYSFGVVALEIIGGKHPGDLLSSLNWSSSHDAVLGSILDKRLAYPTDRSIEKEIMCVYNVALRCVLTDPKCRPTMRNVSEELSSEIPEELMYVQTLENLILDFNYLTGSIPASLSNCTNLNWISLSNNKLFGEIPAALGVLPNLAILRLGNNSFSGQIPAELGDCKSLVWLDLNTNQLSGTIPPALFKQSGFVASAYLTGLQYVYVKNDGSRQCHGAGNLLEFGGIRRQDLDRLSSRNPCNYTRVYFGITQPNFNLNGLMIFLDLSYNKLDGGIPKELGSMYYLNLLNLGHNNLTGPIPEELGGLKNTAILDLSHNRLNGSIPGSLTSLGLGDADFSNNNLSGIIPQSAPLDTFPADRFSNNSGLCGYPLHECGSGPKHKSNDQRENMRKNKLVHHLTIFLPIIGGLCFLFLVFMCYKIRKAGKKKIKPETTRHGNVCSVLNYDGRIAYEDFITATEDFDLKYCIGTRGYGSVYKAKLPEGKTFALKKLHRFEAEQFNKSFMNEVQVLTNLRHKNIVKLYGFCLHNKCNFLVYEYIERGSLFCALSNSDLAVEVDWLKRVNIIKNVAHALSYMHHDCSPPIVHRDISTNNILLNSEMQGFVADFGVARLLDPYSSNQTAIAGTLGYIAPELAYNMIVTEKCDVYSFGVVALEIIGGKHPGDLLSSMNWSTSHDAMLANILDTRLAYPSDRSIEKEIMRVYNVALRCVLTDPKCRPTMRNVSEELSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CHY5</accession>
    <name>A0AAD5CHY5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_020162</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008332">
        <property type="protein sequence ID" value="KAI7740856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CHY5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20653">
        <property type="entry name" value="CYP81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000023">
        <property type="entry name" value="Cytochrome P450 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050651">
        <property type="entry name" value="Plant_Cytochrome_P450_Monoox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47947">
        <property type="entry name" value="CYTOCHROME P450 82C3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47947:SF24">
        <property type="entry name" value="ISOFLAVONE 2'-HYDROXYLASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9 10" id="KW-0349">Heme</keyword>
    <keyword evidence="6 9 10" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 9 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 10" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="9">
        <location>
            <position position="439"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7740856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="503" mass="57286" checksum="EA122E5DE878FE47" modified="2024-05-29" version="1">MEVVYLFISLFLLLASYLLITHIHHKSANLPPTVFPALPVIGHLYLLKKPLYRTLAKISAKYGPVLFLRLGFRRVLLISSPSAAEECFTKNDIIFANRPHLLFGKIIGNNYTSLGWSSYGDNWRNLRRIASTEILSTPRLNEFHDIRAEEGRLLVRKLMSNSSPEGVKMRSVFYEVTLNVMMRMISGKRYFGGDIPGAEEEGQRLQEMMEETFLIHGAGNVGDYLRVSWLGVKGLEKRLIALQEKLDDFIQKLIEQLRTSKGEMKTKTRKTMVEVLLSLQESDPEYYTDQMIRSFVQVMIFAGTDTSSGTMEWCLSLLLNHPQVLRKAQNEIDRKVGKDRLVRESDMVDLPYLRGIINETFRLHPAVPVLPPHESSQDCIIEGYNVPCGTMLLVNQWAIQHDAKFWTDPERFNPERFEGIEGMRDGFKLMPFGSGRRGCPGEGLAVLMIGLTLGLLIQCFDWERLSDKMVDMKEGLGVTMPKAEPLVALCKPRLEMQNVLAQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CIJ4</accession>
    <name>A0AAD5CIJ4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_008882</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008107">
        <property type="protein sequence ID" value="KAI7741900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CIJ4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46620">
        <property type="entry name" value="J DOMAIN-CONTAINING PROTEIN SPF31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46620:SF1">
        <property type="entry name" value="J DOMAIN-CONTAINING PROTEIN SPF31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="2">
        <location>
            <begin position="194"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="352"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="352"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="401"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7741900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="426" mass="49609" checksum="27E814922A2B8457" modified="2024-05-29" version="1" fragment="single">VRGLSGASFFGNRRWLVTTVGDVDALLLTDTGPVFKVFLIRLYSILATVADIGIWWLIDAHDAYNLFLIRGCCVSLCFPRSLFLAVSCVSMGQKRAFAPSNQLFGGVWKRLQAWICCSLVIVYHCFVISLANCRPSSIDDDLLLKKEAEQIVRMGEANTSSIDDDLLLKNFYAEVSEVERDNEVVRILSCFKLNAFEYLNLPFDSAPEDVKKQYRKLSLLVHPDKCKHPQAKEAFSALAKAQQLLLDPQEREYLVNQINAAREELRAKRKKQLKKDTASKLKSLVDEVIKLLFTFSRMTVNLRKNLHYITLGKYEQEYEKSAEYQQQLKLKVREILTDQEWRRRKMQMRISEEEGRLKKDEEETKEMWKRKREHEEQWEGTRENRVSSWRDFMKGGKKVKKGELRPPKLKTEDPNKSYVQRPVKRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CKF8</accession>
    <name>A0AAD5CKF8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Rieske domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">M8C21_010658</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">AA19_3_7</strain>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007883">
        <property type="protein sequence ID" value="KAI7742845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CKF8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010277">
        <property type="term" value="F:chlorophyllide a oxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03480">
        <property type="entry name" value="Rieske_RO_Alpha_PaO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.380.10">
        <property type="entry name" value="Naphthalene 1,2-dioxygenase Alpha Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.10.10">
        <property type="entry name" value="Rieske [2Fe-2S] iron-sulphur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050584">
        <property type="entry name" value="Cholesterol_7-desaturase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013626">
        <property type="entry name" value="PaO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036922">
        <property type="entry name" value="Rieske_2Fe-2S_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21266:SF32">
        <property type="entry name" value="CHOLESTEROL 7-DESATURASE NVD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21266">
        <property type="entry name" value="IRON-SULFUR DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08417">
        <property type="entry name" value="PaO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00355">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50022">
        <property type="entry name" value="ISP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="3" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0934">Plastid</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Rieske" evidence="14">
        <location>
            <begin position="53"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="15">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="15">
        <location>
            <position position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAI7742845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="431" mass="49732" checksum="3E885D3ECCCE2C07" modified="2024-05-29" version="1" fragment="single">SPLLHFTQLPKSKFTKLNVISPSVSTQPAVPLEETTQTDSCDQDDKFDWFSYWYPLMPVCDLDKRVPTGKKVLGLDVVVWWDRNDNEWKVFEDRCPHRLAPLSEGRIDQWGRLQCVYHGWCFGGSGDCKLIPQAPVDGPSVHSSPKACVGVYPSTVQNEIVWFWPNNNPKHKDVITKKKPPYIPELDDPSFSFHMFNRDIPYGYEVLIENVMDPSHVDREGGSPIDIKIHKIDKNGFTTTEQPDRKWNFVSPCLVQGTLTIKESLDSTKGNFSQTIPRKDLFIFLCVPVSQGNSRLISVFPRNFRIWIDPFVTRWMHHVMQNLVIDSDFYLLRVQEEKLMETSPSNWEKTCFVPTKADGNVVAYRKWLKQYAGGKIDWGTKSIAAVTPMPTREQLLDRYWTHVVNCNSCKGAYKGFKALEIFLQQKSYRKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CMK7</accession>
    <name>A0AAD5CMK7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_027633</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007455">
        <property type="protein sequence ID" value="KAI7744721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CMK7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7744721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="78" mass="8431" checksum="0019C1EBC2F1878B" modified="2024-05-29" version="1" fragment="single">VEHPFENFGKNGWSNIEEGYVCCQMVLGYDVSTNDVGVDCDGTAAVHYMAGYDVCVYYVVVECDGASALQPIDLPLCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CMQ1</accession>
    <name>A0AAD5CMQ1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein groES</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_022611</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Seems to function only as a co-chaperone, along with cpn60, and in certain cases is essential for the discharge of biologically active proteins from cpn60.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Forms stable complexes with CPN60 in the presence of ATP.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the GroES chaperonin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007422">
        <property type="protein sequence ID" value="KAI7744888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CMQ1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00320">
        <property type="entry name" value="cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.33.40:FF:000002">
        <property type="entry name" value="10 kDa chaperonin, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.33.40">
        <property type="entry name" value="GroES chaperonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020818">
        <property type="entry name" value="Chaperonin_GroES"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037124">
        <property type="entry name" value="Chaperonin_GroES_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10772">
        <property type="entry name" value="10 KDA HEAT SHOCK PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10772:SF37">
        <property type="entry name" value="GROES-LIKE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00166">
        <property type="entry name" value="Cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00297">
        <property type="entry name" value="CHAPERONIN10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00883">
        <property type="entry name" value="Cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00053355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00062160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00083733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7744888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="196" mass="21579" checksum="CC3CE6A8FD84C02E" modified="2024-05-29" version="1" fragment="single">AGLVMMKREWAGPPVIADHQPGSSRLIPLFNRVLVEKIIPPSKTTAGILLPEKASKLNSGKVIAVGTGTHDLSGNKIPVGVKEGDTVLLPEYGGTEVKLGEKEYVLLDLWRLWLRGSRSALLFQIGIIYTGMRTFWELCMIKFSQAVIIFLPGFIKLDVLILINGLKDLLANEMVLYSKKAKGVNESSLSLTRHEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CNH8</accession>
    <name>A0AAD5CNH8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Prephenate/arogenate dehydrogenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_013813</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007544">
        <property type="protein sequence ID" value="KAI7744360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CNH8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033730">
        <property type="term" value="F:arogenate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008977">
        <property type="term" value="F:prephenate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004665">
        <property type="term" value="F:prephenate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006571">
        <property type="term" value="P:tyrosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046826">
        <property type="entry name" value="PDH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003099">
        <property type="entry name" value="Prephen_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045011">
        <property type="entry name" value="TYRAAT1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059064">
        <property type="entry name" value="TYRAAT2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43207:SF3">
        <property type="entry name" value="AROGENATE DEHYDROGENASE 1, CHLOROPLASTIC-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43207">
        <property type="entry name" value="AROGENATE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02153">
        <property type="entry name" value="PDH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26213">
        <property type="entry name" value="TYRAAT1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51176">
        <property type="entry name" value="PDH_ADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Prephenate/arogenate dehydrogenase" evidence="3">
        <location>
            <begin position="10"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="231"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7744360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="263" mass="29852" checksum="514D5EC33A151E9D" modified="2024-05-29" version="1">MVMGTSTRTLNIGIIGFGPFAQFLAETMIKQDHVVRATSRSDYSALLGPKFFRDMEEFMKVGHDVIMICTSILSVSEVVKSIPFHCLKKPTLFADVLSVKEHPRQILLQEVPEDSDVVCTHPMFGPESGKDGWNGLNFMYERVRVRDEDVCSRFLNIFESEGCKMIEMTCEEHDRLAAQSQFVTHTIGRILSEMQITNTPIDTKGFEKLVQVKENTANDSLDLYTGLFIHNRFAKQQMKNLEHALERVKGSLEASMKNEDGHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CT07</accession>
    <name>A0AAD5CT07_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_011046</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006899">
        <property type="protein sequence ID" value="KAI7746745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CT07"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7746745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="166" mass="18409" checksum="B04930511F7B365A" modified="2024-05-29" version="1">MLFSILSHINAITPSALFQRVSAVFQRVSALFQRVSERFSAFRSCFSVVQRCFRAVSARFSAVSARFSAFQSCFSAVSARFSVILRVSDMVVADLAGNNRSAATSNNKFVDIRNWVLQKVEYGKVRLVYRAICANTTCKPLLPLAEVGKGSSQHAKFHMTCLSSHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CT37</accession>
    <name>A0AAD5CT37_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">M8C21_024655</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">AA19_3_7</strain>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="10">Part of the t-SNARE complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the syntaxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007046">
        <property type="protein sequence ID" value="KAI7746090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CT37"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048278">
        <property type="term" value="P:vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15848">
        <property type="entry name" value="SNARE_syntaxin1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00179">
        <property type="entry name" value="SynN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000008">
        <property type="entry name" value="Syntaxin 132"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.70:FF:000013">
        <property type="entry name" value="Syntaxin 132"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010989">
        <property type="entry name" value="SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045242">
        <property type="entry name" value="Syntaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006012">
        <property type="entry name" value="Syntaxin/epimorphin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006011">
        <property type="entry name" value="Syntaxin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957:SF307">
        <property type="entry name" value="PROTEIN SSO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05739">
        <property type="entry name" value="SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00804">
        <property type="entry name" value="Syntaxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00503">
        <property type="entry name" value="SynN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47661">
        <property type="entry name" value="t-snare proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00914">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="8 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="279"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="15">
        <location>
            <begin position="207"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="37"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00061857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAI7746090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="308" mass="34886" checksum="7EE4609383B470CF" modified="2024-05-29" version="1">MNDLLSESFDNPRGQDNGYGDLEMGTQRNADSGELGLDDFFKKVQAIEKQYEKLNKLLKKLQDAHEESRTVTKAAAMKAIKQRMEKDVDEVGKIARFIKAKIEELDKENLANRQKPGCGKGTGVDRSRTATTVSLKKKFKDKMTEFQTLRESIHQEHREVVERRVYTVTGTRADEETIDRLIETGDSEQIFQKAIREQGRGQVLDTLAEIQERHEAVREVERKLHDLQQIFMDMAVLVDAQGEMLDNIETQVSSAVDHVQDGNKALHKAKSLQKNSRKWMCIAILILLVIIAVVVVGVLKPWKSGNGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CT49</accession>
    <name>A0AAD5CT49_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Transcription factor-like protein DPB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_020893</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the E2F/DP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007045">
        <property type="protein sequence ID" value="KAI7746105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CT49"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14458">
        <property type="entry name" value="DP_DD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.80">
        <property type="entry name" value="Transcription factor DP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037241">
        <property type="entry name" value="E2F-DP_heterodim"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003316">
        <property type="entry name" value="E2F_WHTH_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038168">
        <property type="entry name" value="TF_DP_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014889">
        <property type="entry name" value="Transc_factor_DP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015648">
        <property type="entry name" value="Transcrpt_fac_DP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12548">
        <property type="entry name" value="TRANSCRIPTION FACTOR DP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12548:SF9">
        <property type="entry name" value="TRANSCRIPTION FACTOR DP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08781">
        <property type="entry name" value="DP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02319">
        <property type="entry name" value="WHD_E2F_TDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01138">
        <property type="entry name" value="DP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01372">
        <property type="entry name" value="E2F_TDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144074">
        <property type="entry name" value="E2F-DP heterodimerization region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 8" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="E2F/DP family winged-helix DNA-binding" evidence="10">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription factor DP C-terminal" evidence="9">
        <location>
            <begin position="64"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7746105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="149" mass="17104" checksum="7AAB8ABCF3463500" modified="2024-05-29" version="1" fragment="single">VADELVAEFSDPGYGDQAPDQKQYDEKNIRRRVYDALNVLMAMDIISKDKKEIQWRGLPQTSQNDIEELKSERLALKNRIEKKTAYLGELEDQHVGLQNLIKRNEQLYSSGNTPSGGVVLPFILVQTRPHATVEMEISEDMQLVHFDFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CTE4</accession>
    <name>A0AAD5CTE4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_012066</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006733">
        <property type="protein sequence ID" value="KAI7747554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CTE4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026960">
        <property type="entry name" value="RVT-Znf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13966">
        <property type="entry name" value="zf-RVT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase zinc-binding" evidence="1">
        <location>
            <begin position="46"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7747554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="116" mass="13180" checksum="4020DD5CAD80BCB1" modified="2024-05-29" version="1">MKQTLRSLDKNHLKLNGRSETRVASQLHLSQHALTSYGSLHKACSNSEAIHNFRCRIKWVNWVPLKCNVMAWRASMGHLPVKTELLKRNVQVDNQVASMGGDLLYRGILTISLTTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CUJ6</accession>
    <name>A0AAD5CUJ6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_012650</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006581">
        <property type="protein sequence ID" value="KAI7748184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CUJ6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008507">
        <property type="entry name" value="DUF789"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31343:SF65">
        <property type="entry name" value="DUF789 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31343">
        <property type="entry name" value="T15D22.8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05623">
        <property type="entry name" value="DUF789"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="162"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="163"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7748184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="374" mass="42029" checksum="4B8683596FD8EEB6" modified="2024-05-29" version="1">MLGAGLQFGRSRNGEDRFYNPAKARRNRQVQDNLRRAQSDVTPTSSGPENRAVLKPLKPVPVEPPVTSPVCNLERFLESVTPSVPAQYPSKRAMGGWRNCDDYQPYFVLGDLWESFKEWSAYGAGVPLILNDSDSVVQYYVPYLSGIQLYIDPLKSSAKSRHHTEDSDVDSFRDSSSDVSSDFDLPKSEVTQRLDQLSLNYNHALQEGFSSDEGESATPQSCLSYEYMERNQPWGREPLTDKILDLALRFPELKSMKSCDLLSSSWLSVAWYPIYRIPMGPTLKDLDACFLTFHSLHTPIAGNQCEHPPAVTHSGETGGPKISLPVFGLASYKLKAPLWINNESALLTDLLQEADSWLANLQVNHPDFLFFSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-10-08" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CW37</accession>
    <name>A0AAD5CW37_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_030097</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006415">
        <property type="protein sequence ID" value="KAI7749054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009914">
        <property type="term" value="P:hormone transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000032">
        <property type="term" value="P:regulation of secondary shoot formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03233">
        <property type="entry name" value="ABCG_PDR_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03232">
        <property type="entry name" value="ABCG_PDR_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000179">
        <property type="entry name" value="ABC transporter G family member 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000059">
        <property type="entry name" value="ABC transporter G family member 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034001">
        <property type="entry name" value="ABCG_PDR_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034003">
        <property type="entry name" value="ABCG_PDR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013581">
        <property type="entry name" value="PDR_assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48040:SF45">
        <property type="entry name" value="PLEIOTROPIC DRUG RESISTANCE PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48040">
        <property type="entry name" value="PLEIOTROPIC DRUG RESISTANCE PROTEIN 1-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19055">
        <property type="entry name" value="ABC2_membrane_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08370">
        <property type="entry name" value="PDR_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="492"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="525"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="576"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="610"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="635"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="718"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1125"/>
            <end position="1143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1211"/>
            <end position="1231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1238"/>
            <end position="1262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1268"/>
            <end position="1287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1299"/>
            <end position="1318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1345"/>
            <end position="1368"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="123"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="791"/>
            <end position="1031"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="1376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7749054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="1376" mass="155917" checksum="97E19B37147932AF" modified="2024-05-29" version="1" fragment="single">GGDVFRVSSARISSSNIWRNSGRDIFSRSSVEEDDEEALKWAAIEKLPTNLRLERGILTEETGQPREIDIKSLDLVEKRNLLERLVKIAEEDNEKFLLKLKNRIDRALPTIFNFLVNILEGSLNYLHLFPSRKIPLPVLHDVSGIIKPGRMTLLLGPPSSGKTTLLLALAGKLGSDLKVSGRVTYNGHEMNEFVPQRSSAYISQNDLHIGEMTVRETLAFSARCQGVGASYEMLAELSRREKEANIKPDPDLDIYMKAASLEGQEASVVTEYVIKILGLEVCADTIVGDEMFRGISGGQKKRVTTGEMLVGPARALFMDEISTGLDSSTTFQIVNSIRQSIHILQGTSVISLLQPAPETYDLFDDIILLSDGQIVYQGPRENVLEFFEYMGFKCPERKGVADFLQEVTSRKDQEQYWTKRDQPYSFITSREFAEAFQSFHIGKQLGDELSTPFERSRSHPHALTTKKFGVSKKELLKACISREYLLMKRNSFVYIFKMTQLIFMASITMTLFLRTEMPKETFVDGTIFMGALFFTLVMITFNGFSELSLSILKLPVFYKQRDLLFFPAWAYSLPTWVLKIPVTIVEVATWVIMTYYVIGFDSDPARFFKQMVLLVCIHQMSSGLFRFIGSIGRNVIVANTFGSFGLLTIFVLGGFILARDDIRGWWVWCYWFSPMMYGQNGIAVNEFLGDSWNKVLPNSTETIGVSVLKSRGIFPEAYWYWIAVAASIGYMFLFNICFTLALQFLNRNNLQKSVNYHTFVAAFGNPQAVMSEESMAARKAMKTGHAIELSARPPSAQGVAQDRLELLKGVSGSFRPGVLTALMGISGAGKTTLMDVLAGRKTGGYIEGAITISGYPKKQETFARIAGYCEQTDIHSPHVTVYESLQYSAWLRLPPEVDSATREMFVEEVMQLVELVPLREALVGLPGVNGLSTEQRKRLTIAVELVANPSIIFMDEPTSGLDARAAAIVMRTVRNTVDTGRTVVCTIHQPSIDIFDAFDELFLLKRGGEEIYVGPLGRHSSHLIKYFEGIDGVSKIKDGYNPATWMLEVTSTGQEATLKVNFAEVYKNSELYKRNKELIKETSKPIPGSSDLHFRSQYSQSFWTQCMACLWKQNWSYWRNPPYTAVRFLFTTFIALMFGTIFWDMGSKREKTQDLFNAMGSMYAAILFIGVQNATSIQPVVSIERTVFYRERAAGMYSALPYAFGQVMIEIPYIFVQTIVYGIIVYAMIGFEWTAVKFFWYLFFMYFTFLYFTFYGMMTVAVTPNHNFAAIISSAFYAIWNLFSGFIVPRTRIPIWWRWYYYICPIAWTLYGLVTSQFGDITDKLETGETVAEFVHSFYGFEYGFIKYVAVIIVGFTVLFGFIFAYSIKAFNFQKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CWH7</accession>
    <name>A0AAD5CWH7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_002928</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006579">
        <property type="protein sequence ID" value="KAI7748219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CWH7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038971">
        <property type="entry name" value="SMR11/SMR16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36310">
        <property type="entry name" value="CYCLIN-DEPENDENT PROTEIN KINASE INHIBITOR SMR11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36310:SF1">
        <property type="entry name" value="CYCLIN-DEPENDENT PROTEIN KINASE INHIBITOR SMR11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7748219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="175" mass="19414" checksum="DE61C808154674AF" modified="2024-05-29" version="1">MSFDQMDIIENLEKTSDKNSTLMEPKNSDLDASLCPITPYLTKEKVNVTSTPVEDVFDMRVSGSNQTNNLGTSVVARKKLEFSSDECVSEEEIENGSLMETVYESVLEEIICKQAEDILAEILVSDSSRDVLKTPPSVIALAEKCPGAPLKPKQVRNKRTVDMSLCRKLDFDSCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CWR9</accession>
    <name>A0AAD5CWR9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RanBP2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_015785</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006636">
        <property type="protein sequence ID" value="KAI7747936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CWR9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1060.10:FF:000023">
        <property type="entry name" value="Ran-binding zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1060.10">
        <property type="entry name" value="Zinc finger, RanBP2-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036443">
        <property type="entry name" value="Znf_RanBP2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23111:SF74">
        <property type="entry name" value="OS02G0203700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23111">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00641">
        <property type="entry name" value="Zn_ribbon_RanBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00547">
        <property type="entry name" value="ZnF_RBZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90209">
        <property type="entry name" value="Ran binding protein zinc finger-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01358">
        <property type="entry name" value="ZF_RANBP2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50199">
        <property type="entry name" value="ZF_RANBP2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RanBP2-type" evidence="5">
        <location>
            <begin position="7"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="domain" description="RanBP2-type" evidence="5">
        <location>
            <begin position="58"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7747936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="174" mass="18178" checksum="E35E92790AC9318D" modified="2024-05-29" version="1" fragment="single">KLIKMSRPGDWNCRSCQHLNFQRRESCQRCGEMKHGGGFGGRGSINASAFGFTGPDVRPGDWYCNVGNCGAHNFASRSSCFKCGAFKDDLASSGGGGGFDGDMSRSRGFGFGGSGSGGSSRSGWKSGRAATSTTLLAEWSALDATRLGIPVAILPFKPAVCVISSSGVFRSARK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-02-05" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CY28</accession>
    <name>A0AAD5CY28_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SHSP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_006277</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006344">
        <property type="protein sequence ID" value="KAI7749535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031107">
        <property type="entry name" value="Small_HSP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11527">
        <property type="entry name" value="HEAT-SHOCK PROTEIN 20 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="SHSP" evidence="4">
        <location>
            <begin position="43"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7749535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="154" mass="17109" checksum="F2389427A8C099CD" modified="2024-05-29" version="1">MSIIPSFFTGSRSNIFDPFSSEIWDPFQGFSSVLNNLPESARETTAIANARVDWKETPEAHVFKADLPGLKKEEVKVEVEEGRVLQIXXXXXXXXXXXXXXXXXXXXXXXXXXXLPENAKMDQVKAAMENGVLTVTVPKEEEKKPEVKAIDISG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CYI2</accession>
    <name>A0AAD5CYI2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">lycopene beta-cyclase</fullName>
            <ecNumber evidence="3">5.5.1.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_010890</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Carotenoid biosynthesis; beta-carotene biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7">Carotenoid biosynthesis; beta-zeacarotene biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the lycopene cyclase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.5.1.19" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010006291">
        <property type="protein sequence ID" value="KAI7749722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CYI2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016860">
        <property type="term" value="F:intramolecular oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016117">
        <property type="term" value="P:carotenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010108">
        <property type="entry name" value="Lycopene_cyclase_b/e"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01790">
        <property type="entry name" value="carotene-cycl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39757">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39757:SF5">
        <property type="entry name" value="OS02G0190600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05834">
        <property type="entry name" value="Lycopene_cycl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0125">Carotenoid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7749722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="326" mass="37014" checksum="B72B151E4F2686EB" modified="2024-05-29" version="1">MLQKCIANGVKFHQAKVIKVIHEESKSLLICNDGVTIQATLVLDATGFSRSLVQYDKPYNPGYQVAYGILAEVEEHPFDVDKMLFMDWRDSHLKNNLEIKERNSKIPTFLYAMPFSSTRIFLEETSLVARPGLKMEDIQERMVCRLKHLGIKVKSIEEDERCVIPMGGPLPVLPQRVLGIGGTAGMVHPSTGYMVARTLAAAPIVAKSIIRYLDNEKKASGDDLSAGIWRELWPIERRRQREFFCFGMDILLKLDLEGTRRFFDAFFDLEPRYWHGFLSSRLFLPELVTFGLSLFAHASNTCRVEIMAKGTLPLATMIGNLVQDRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D5G9</accession>
    <name>A0AAD5D5G9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_005091</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005691">
        <property type="protein sequence ID" value="KAI7752421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D5G9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11017">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11017:SF585">
        <property type="entry name" value="TIR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7752421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="131" mass="15517" checksum="DB48788508192395" modified="2024-05-29" version="1" fragment="single">MMHQLLQDMGRDIVRQESPKKPWKRSILWHLEECLDVLQNRQGTPRVQGLVLDMRTFDIETSKEPSSANMQNFGFRSFALYNWVQILLSVIWWLFAWVLETRSSSCKTKGNFLTLPLSEMRNLKLLQLNYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D671</accession>
    <name>A0AAD5D671_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glucose-methanol-choline oxidoreductase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_004899</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005280">
        <property type="protein sequence ID" value="KAI7753757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D671"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016614">
        <property type="term" value="F:oxidoreductase activity, acting on CH-OH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.410.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.560.10">
        <property type="entry name" value="Glucose Oxidase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051871">
        <property type="entry name" value="GMC_Oxidoreductase-Related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000172">
        <property type="entry name" value="GMC_OxRdtase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45968:SF36">
        <property type="entry name" value="(R)-MANDELONITRILE LYASE 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45968">
        <property type="entry name" value="OSJNBA0019K04.7 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00732">
        <property type="entry name" value="GMC_oxred_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00624">
        <property type="entry name" value="GMC_OXRED_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucose-methanol-choline oxidoreductase N-terminal" evidence="4">
        <location>
            <begin position="152"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="Glucose-methanol-choline oxidoreductase N-terminal" evidence="4">
        <location>
            <begin position="375"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS00624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7753757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="554" mass="60569" checksum="C0683DC1D988238F" modified="2024-05-29" version="1">MINFGFYSRADDYFYENSGIEWDMSAVKSAYEWVENSIVTRTDRLRRWQASTYNAFVEAGVAPDNGFILDHVQGTKIGGSTYDDSGRRHGAVELLSKANPENIKVVVHATVDRVMFSNSKPLAAVGVKYHDSKGTQHEVHVRRGGEVILSAGAIGSPQLLLLSGIGPESSLSSMDIPVVRDQPFIGQFMADNPRNGVNLLVPVRLADVGVRVAGITNIGPYVESTAVPRVSSIIPFIPFLDLIPPLNLSVVVVGGKVSRPKSTGSLNLVSPVDVTVSPKVRFNYYSHTEDILQCGNLVEVLRKVLETQAMEEYKFPGILVPNYFTYIGPSLPEDSSDQESLATFCHAVGVNYHDSNGTRHEVHVRRSGEVILSVGAIGSPKLLLLSGIGPCNGVNLLVSIRLADVGVRVAGIIDIRPYVESTTVTRVSSIIPFLDLIPPINLSVVVVGGKVSRTKSTGSLNLVSPVDVTVSPKVWFNYYSRTEDIPQCGNLVEVLRKVLETQAKDKYKFPRILGPNYFTYISPSLPMDSSDQESLATFRRKHCQHLGICMVDAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D7B8</accession>
    <name>A0AAD5D7B8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CMP/dCMP-type deaminase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_006030</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010003865">
        <property type="protein sequence ID" value="KAI7754342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D7B8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008251">
        <property type="term" value="F:tRNA-specific adenosine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002100">
        <property type="term" value="P:tRNA wobble adenosine to inosine editing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11079">
        <property type="entry name" value="CYTOSINE DEAMINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11079:SF179">
        <property type="entry name" value="TRNA(ADENINE(34)) DEAMINASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00383">
        <property type="entry name" value="dCMP_cyt_deam_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="Cytidine deaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="2">
        <location>
            <begin position="914"/>
            <end position="955"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="220"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="247"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="277"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="741"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="220"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="247"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="279"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="303"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="331"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="342"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="367"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="398"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="425"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="444"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="498"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="508"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="530"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="562"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="635"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="756"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="768"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="796"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="834"/>
            <end position="850"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7754342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="955" mass="106751" checksum="E451CC7934D76A7A" modified="2024-05-29" version="1">MMYTTCLSPSVSLRCRASQSLYLNDPTFFTCSTFQTNPLLISSSSSINSCCCCCCCGNNNNNNLVYNSLPRIKPSFLCNGLRQSSLIQFSPTRRLVVDGNYSFSLSSESDGKSCYKKICSFKDRRRRLGGRRYACRSYEGKKDEGCFSKGIGDDCGDEVELLLDLLTEEVGLESVSVRGRKRVEKKDSSKVSGRAKAVKSGSVKRDLKCDAKVVEVRSSERKDRKLSDERRSSVTGVENKVKTQGSSCSSYYSVSSAGENDSDNDVVIENNGYFVKGESSSEYKDDQKVDRHSSVYDEELEENVERQQEFGKKESEIVKKNAAESYYGNQEWRKKSEKKLNVESSQVQRTERNSQYYSEKSDRITDQSRSGMKNTNASTQKLYSGAENKSSTSAQELTSHRKGETVDITRQNEYKKQSNVNLEASEIRNLDKRMASSSSSSFETRMKNREENSSQVSDRFEGQREDKHQRVVQLASSKESRMKSQQISETSDTHVTDVENTLVTQSDIRTKKQELHDQREEKHQRVDQVQAASSKESRVKSQQISDTRVTNIENTSVSHTQSDIRTKTQDLHLESSSSSEKRENIEDKYSSSQVATVAGPTETTKTGRKTTKASSFHVGMPKETSSSYKALRLNPESSFQETGTHIASADEQHKSAGQFVGEFVEKAKHQLSISEGELAREDDEQHELKSSGESGSGNYDEKDGDMGPSDEMWHESGAPDEQVKRSGRSIWNVIGDVVRLRWGPSRSETHTPKSGGVSNQSTSSERWFSGHEPDELSRKDVSTTSSFKEKQDSPLLISSSNTNQGSSPKSMSPIVIEESSNPLPAIRMRRSPVVKTTSVTGETDASTSGKMDQRVSKPLTQVPVPQTDASGSGKMVTVEPPIPSRRKLARIDQVSKDRFDEWEEAYTVEAKQRKNDEFFMREALSEAKKAADMWEVPVGAVLVQDGKIIARGYNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D7M7</accession>
    <name>A0AAD5D7M7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_018910</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010002421">
        <property type="protein sequence ID" value="KAI7754817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D7M7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7754817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="131" mass="13781" checksum="71B97F61376DA231" modified="2024-05-29" version="1" fragment="single">MWLPGVCINDLLAEVAVCDFGAGVAGCGVAEDPCSISSCRKHALLTVQQMSSFAARSNKTASMLSLLAEIVHGVFKGTINCAIPTTALFIYTFVACRAACVVPALAKVYLSYSTVDCEGKEEHKRTCDVAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D7S7</accession>
    <name>A0AAD5D7S7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Caffeoyl-CoA O-methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_003102</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-dependent O-methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005321">
        <property type="protein sequence ID" value="KAI7753696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D7S7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009809">
        <property type="term" value="P:lignin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050362">
        <property type="entry name" value="Cation-dep_OMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002935">
        <property type="entry name" value="SAM_O-MeTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10509:SF82">
        <property type="entry name" value="CAFFEOYL-COA O-METHYLTRANSFERASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10509">
        <property type="entry name" value="O-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01596">
        <property type="entry name" value="Methyltransf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51682">
        <property type="entry name" value="SAM_OMT_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0438">Lignin biosynthesis</keyword>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7753696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="229" mass="25833" checksum="D0A335E40E0A9513" modified="2024-05-29" version="1">MEHETCLLQSDELYKYILETNVYPHEPEPLKEIRALTASQPLFFMGTSPDAGQMMEMLLKLTGAKKTIEVGVYTGYSLLLTALTIPEDGKIVAIDINREYYEIGRPVIEKAGVEHKIDFIESDAITALDKLLEDHDNEGSFDYVFVDADKTNYLNYHERLLKLVKVNGVIVYDNTLWYGSVAKSENSVPEILKEGRISILKFNKALVDDPQVKISMAPLGDGITICRRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D9F8</accession>
    <name>A0AAD5D9F8_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_030947</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily. Type-7 methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000905">
        <property type="protein sequence ID" value="KAI7755672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D9F8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.270">
        <property type="entry name" value="Methyltransferase, alpha-helical capping domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005299">
        <property type="entry name" value="MeTrfase_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042086">
        <property type="entry name" value="MeTrfase_capping"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31009">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE:CARBOXYL METHYLTRANSFERASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03492">
        <property type="entry name" value="Methyltransf_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7755672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="207" mass="23375" checksum="ACD3C9B8E7C15EC6" modified="2024-05-29" version="1">MPQGLESNGLNIYMAKTSPTNVYEAYGKQFHTDFTKFLQMRSKEVVHGGSMVLTFPGRSMVDPTSEDSCALLGLLGQSLVDISREGLVQEQDIISFNIPMYTPCENEVRNAIENEGSFSITNMNNFKVNWDPNDTDFTSMNDSNELNQIHGKNTAKMMRAVLEPLLASHFGNSIIDVLFKKLEKNVAEYLSKKKPRQFFINISMTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DA93</accession>
    <name>A0AAD5DA93_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Germin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">M8C21_033534</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">AA19_3_7</strain>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">May interact with bacterial adhesins thereby protecting the reproductive tissues from microbial attack. Has no oxalate oxidase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 19">
            <text>2 superoxide + 2 H(+) = H2O2 + O2</text>
            <dbReference type="Rhea" id="RHEA:20696"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:18421"/>
            <dbReference type="EC" id="1.15.1.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Monomer. In the absence of manganese, it forms tetrameric and pentameric forms which show superoxide dismutase activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Secreted</location>
            <location evidence="1 15">Extracellular space</location>
            <location evidence="1 15">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the germin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000793">
        <property type="protein sequence ID" value="KAI7755824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DA93"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009506">
        <property type="term" value="C:plasmodesma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004784">
        <property type="term" value="F:superoxide dismutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010497">
        <property type="term" value="P:plasmodesmata-mediated intercellular transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000280">
        <property type="term" value="P:regulation of root development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02241">
        <property type="entry name" value="cupin_OxOx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000025">
        <property type="entry name" value="germin-like protein subfamily 2 member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006045">
        <property type="entry name" value="Cupin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001929">
        <property type="entry name" value="Germin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019780">
        <property type="entry name" value="Germin_Mn-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31238">
        <property type="entry name" value="GERMIN-LIKE PROTEIN SUBFAMILY 3 MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00190">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00325">
        <property type="entry name" value="GERMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00835">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00725">
        <property type="entry name" value="GERMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 15" id="KW-0052">Apoplast</keyword>
    <keyword evidence="7 14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8 12 15" id="KW-0464">Manganese</keyword>
    <keyword evidence="5 12 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 15" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 15" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041767798" description="Germin-like protein" evidence="15">
        <location>
            <begin position="26"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="Cupin type-1" evidence="16">
        <location>
            <begin position="61"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="34"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00058969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00064720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU366015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAI7755824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="219" mass="23250" checksum="CD4EC0CDFC31D253" modified="2024-05-29" version="1">MSTLSDYGLIIGVMIAISLASFASADPELLQDVCVADLSSGVKMNGFACKSNISADDFFFAGLAKPGLTNNTFGATVTPASVQQITGLNTLGVSMARIDYAPGGLNPPHTHPRATEIIFVLEGELDVGFITTENKLFSKTIKKGEIFTFPRSLIHFQINNGKVPAAVIAAFNSQLPGTQRVANAVFASSPTVEDVVLTKTFQVGTKQVEKIKSRLAPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DBI2</accession>
    <name>A0AAD5DBI2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BSD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_011106</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000423">
        <property type="protein sequence ID" value="KAI7756364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DBI2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3970.10">
        <property type="entry name" value="BSD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005607">
        <property type="entry name" value="BSD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035925">
        <property type="entry name" value="BSD_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31923">
        <property type="entry name" value="BSD DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31923:SF4">
        <property type="entry name" value="BSD DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03909">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00751">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140383">
        <property type="entry name" value="BSD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50858">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BSD" evidence="2">
        <location>
            <begin position="147"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="340"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="353"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7756364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="379" mass="42871" checksum="46D6E81ADDC4DE4F" modified="2024-05-29" version="1">MSWLPRSITNNLKQPVTDDNSVKSPTTGVKQDLSEISQTLTRHFCRVASFIAPNERSEPLDLEAVAGMRRDLAEIGGRFRNGISKVSEFLDVESDDDDDDYYYYYSSDDDGDGEGVVGVTDDVVDFVRDIIMHPETWLDFPLPEDDDEDFDLSDAQREHAFAVQRLVPILRALRIELCPNYMTQSSFWKIYFVLLHPRLDRDAAQLLSTPNIVKARALLTHDLKNRSCSENMSDHKSKCLPLEVSAIETVKRPIKIDDVQIVDKSVIQEDSHEVKDEDDWLKEESPKVITNRVVIPIENNQDVSFSDLEDDDDDGNIPIHYKNATYAADSSTKDSRIRVQLNQSSTDRKGRDSSSQVGIRNSETKGSNDLLDVADIDVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DCS3</accession>
    <name>A0AAD5DCS3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glycosyltransferase</fullName>
            <ecNumber evidence="5">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_026865</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">May glycosylate diterpenes or flavonols in leaves.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="5"/>
    <dbReference type="EMBL" id="JAMZMK010001098">
        <property type="protein sequence ID" value="KAI7755465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DCS3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035251">
        <property type="term" value="F:UDP-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000056">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050481">
        <property type="entry name" value="UDP-glycosyltransf_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48048:SF98">
        <property type="entry name" value="FLAVONOL 3-O-GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48048">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00053747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7755465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="472" mass="53313" checksum="CA98975B2B28CF12" modified="2024-05-29" version="1">MKRAEVAIIATPTMGNLLPTIEFATHLTNHHHHHISITILTISIPQWPLMHNYAQSLTPTDHIRFIPLPPPDTPPPTHYDSLIEYLSLYIQYHKPIVQQILENHPVPLAGFFIDMFCTSMIDVANDLNIPCYLYFASPAAYLAFVLHLTTLPVTESTDSVNKEVEERRLAVPGLINLVPSTAFPLFCNNKKELGYSFFVRHALRYKETKGIVVNTFRELEPYALDSLSNNNIGWPPVYPVGPVVDHVGSVNWHPNRSMHEKIIKWLDEQPDCSVVFLCFGSMGSMNRVQVREIATGLERTGYRFLWSLREPGKTDIELPHDYEKLEDLFPNGFIDRTAHIGLVCGWVSQVSVLAHKAVGGFVSHCGWNSILESISYGVPIATWPLYAEQHLNAFEMVKEVELSVEIRLGKEGDLVLADEVERAVRELMDDEDGGELRKKVKEMSEMSKTALMENGSSFQALENLICNLLSNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DDG6</accession>
    <name>A0AAD5DDG6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Carboxypeptidase</fullName>
            <ecNumber evidence="10">3.4.16.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_009379</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the peptidase S10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.-" evidence="10"/>
    <dbReference type="EMBL" id="JAMZMK010000278">
        <property type="protein sequence ID" value="KAI7756726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DDG6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11320:FF:000001">
        <property type="entry name" value="Carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000013">
        <property type="entry name" value="Carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11320">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033124">
        <property type="entry name" value="Ser_caboxypep_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018202">
        <property type="entry name" value="Ser_caboxypep_ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF31">
        <property type="entry name" value="SERINE CARBOXYPEPTIDASE-LIKE 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00560">
        <property type="entry name" value="CARBOXYPEPT_SER_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00131">
        <property type="entry name" value="CARBOXYPEPT_SER_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 10" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041770171" description="Carboxypeptidase" evidence="10">
        <location>
            <begin position="27"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7756726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="481" mass="54154" checksum="A03E6B7C1B31BCFD" modified="2024-05-29" version="1">MNTSRACSFLLIISTAILRWSVAVYGQQPQPTGRAFLEQKADRVIRLPGQPFVSFKQYAGYVTVNETHGRALFYWFFEAEESPEKKPLLLWLNGGPGCSSIGYGGMEELGPFVAQNDTLKLRLNTHAWNRAANLLFLESPVGVGFSYTNTSSDIKQLGDKVTAQDSYTFLLKWFRRFPQYKSHKFYIAGESYAGHYVPQLADLILEKNKTISDEDRINFKGIMIGNALLDDAADQTGMIDYAWDHAVISDRVYDAVKSKCNFNDSRSSNACDGALEDYFDVYKIIDMYSLYAPSCVDTTPNSTRRPHIHGKVSPYTFSKNKAWHVKPTGYDPCQSQYTESYMNRPNVQAALHANTTKIPYPWAHCSGAITYWNDAPDSVLPVIKRLVAGGIRVWVFSGDTDGRIPVTSTRLSLKKLGLNIVEDWSPWYTRSQVGGWTMTYDGLTFVTIRGAGHQVPTFKPKEALQLIKHFLSNKKLPSAPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DF52</accession>
    <name>A0AAD5DF52_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Methylenetetrahydrofolate reductase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_013092</name>
        <name evidence="8" type="ORF">M8C21_016977</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the methylenetetrahydrofolate reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005657">
        <property type="protein sequence ID" value="KAI7752565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAMZMK010000003">
        <property type="protein sequence ID" value="KAI7758404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DF52"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004489">
        <property type="term" value="F:methylenetetrahydrofolate reductase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00537">
        <property type="entry name" value="MTHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029041">
        <property type="entry name" value="FAD-linked_oxidoreductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004621">
        <property type="entry name" value="Fadh2_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003171">
        <property type="entry name" value="Mehydrof_redctse-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00677">
        <property type="entry name" value="fadh2_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45754">
        <property type="entry name" value="METHYLENETETRAHYDROFOLATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45754:SF3">
        <property type="entry name" value="METHYLENETETRAHYDROFOLATE REDUCTASE (NADPH)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02219">
        <property type="entry name" value="MTHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51730">
        <property type="entry name" value="FAD-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0274">FAD</keyword>
    <keyword evidence="4 7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7752565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7758404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="210" mass="23321" checksum="27148987F2D3A108" modified="2024-05-29" version="1">MKVIEKILESAKAGDGGKPVFSFEFFPPKTEEGVENLFERMERMVAHDPTFCDITWGAGGSTADLTLEIARRMQNLVCVETMMHLTCTNMPVEKIDHALETIKKDGIQNVLALRGDPPRGQDKFVQIQGGFACALDLVEHIRKSYGDYFGIAVAGYPEAHPEVIESNGLATPEAYAKDLAYLKRKVDAGAEVIVTQLFYDVDNFLKFVND</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DFD2</accession>
    <name>A0AAD5DFD2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ARM repeat superfamily protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_013731</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000005">
        <property type="protein sequence ID" value="KAI7758370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DFD2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052587">
        <property type="entry name" value="TELO2-interacting_protein_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057567">
        <property type="entry name" value="TPR_TTI1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057566">
        <property type="entry name" value="TPR_TTI1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049362">
        <property type="entry name" value="TTI1_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18460">
        <property type="entry name" value="TEL2 INTERACTING PROTEIN 1 TTI1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18460:SF3">
        <property type="entry name" value="TELO2-INTERACTING PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24181">
        <property type="entry name" value="TPR_TTI1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24173">
        <property type="entry name" value="TPR_TTI1_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21547">
        <property type="entry name" value="TTI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TTI1 N-terminal TPR" evidence="1">
        <location>
            <begin position="17"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="TTI1 N-terminal TPR" evidence="1">
        <location>
            <begin position="206"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="domain" description="TTI1 C-terminal TPR" evidence="2">
        <location>
            <begin position="993"/>
            <end position="1160"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF24173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7758370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1225" mass="135954" checksum="1913D8DAC58FF728" modified="2024-05-29" version="1">MMMMMAEGHVEGRGSGFSELKGYCLQLLEAIRNPNNKASTTVAVSHLLQFITRSSSHALQPFFDYILFPLLLLLEAAVDCRSPRKEDNKDTPPQISDAVAEGVIQCLEQLLMKCHVGSVDQMVVILKKLTNAAMLSPSEASEEFREGIIRCFKALINGLCPCSDTSCTCNQINGPPMLLDKKYSKSSLTVSQTHKLSSEECLIAFLRSQSATVTVGHWLSLLLKAADVEASRGHTGSSSLRVEAFMTLRVLVAKVGIADQLAFFLPGVISQIGKVLRVSKTMISGAAGSMEAMDQALRGLAEFLMIVFEDEANLSSLDDSEINLNTEKSPLSFLEELRRLPDKKQDQVEVLATKSTHMSPQSEGKRSLHVDRTKDWIETASSHVNKILSAVSPHLCVHPAKRVRQGTMAAIQGLLSTCIRTLKGSRLMLLECLCALVSDDDEEVSEAAQTFLDSLLSSGNHHIERDFADIFNRLFEKLPEVVVGGEQSLAHSQKLLVLIYYSGPQLVRDHLLHSPVAAAQFFDTLTLCLSQNSVFPGSLDKLLLEKPSSVGYLRSITEMKATTFFENEKKTAVESNAFENPSSFKIQNEYDLPSMPPWFSSRNQKLYYALAGIIRLVSLSLIAGARSDGNLSIIKDIPLSYMRKLIGDIRHKEYTKESWQSWYNRTNLGKLVRQASTSACMLNEMIFGLSDQAIDNLKTRFHNKKSIWHVSLNKDIRIQLIDCIGSILHEYLSPEVWNLPLDQSSVGSGDVAVHFFHDNAMLHQVGHLVLENSDYVIDSICRQLRYLDLNPHVPGVLAAILSYIGVAHEILPLLEEPMRSISQELEILGRHQHPELTISFLRAVAEIAKASKIEACSLPSQAELYHKQVKSELSCFENGVPSNSEESEHLTDFNKHKEKLEAIYFKLKESKSYRRTVGSLSMSCITAATPLLSSLKQTSCLVALEIVQDGLMALAEVEKSFKHETNTREILAQAFQSYSYHDLADTLDAENDGTEENRLLPSMNKIWPFLIACIRNGNPLTTRRCAGVISKAVQICGGDFFSRRFHTDGPHLWELLSTSPFQKKPTNLKERRVLQLPYRTSSTSSEDPRAEISDLKVQVAVLEMIAEISRNKNSASAFESVVKKVSGVVVGIACSGVVGLRDASVNALRGLSTIDSDLIWLLLADVYYSKKREDVSQPPVADLPQVHQLLPPPLSSKSYLYVQYGGQSFGFDIDFSAVEYMGKSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DGS4</accession>
    <name>A0AAD5DGS4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Cytochrome b561 and DOMON domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">M8C21_014335</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">AA19_3_7</strain>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">May act as a catecholamine-responsive trans-membrane electron transporter.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000125">
        <property type="protein sequence ID" value="KAI7757430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DGS4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08760">
        <property type="entry name" value="Cyt_b561_FRRS1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1770:FF:000007">
        <property type="entry name" value="Cytochrome b561 and DOMON domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045265">
        <property type="entry name" value="AIR12_DOMON"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006593">
        <property type="entry name" value="Cyt_b561/ferric_Rdtase_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005018">
        <property type="entry name" value="DOMON_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017214">
        <property type="entry name" value="UCP037471"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23130">
        <property type="entry name" value="CYTOCHROME B561 AND DOMON DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23130:SF210">
        <property type="entry name" value="CYTOCHROME B561 AND DOMON DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03188">
        <property type="entry name" value="Cytochrom_B561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04526">
        <property type="entry name" value="DUF568"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037471">
        <property type="entry name" value="UCP037471"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00665">
        <property type="entry name" value="B561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50939">
        <property type="entry name" value="CYTOCHROME_B561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50836">
        <property type="entry name" value="DOMON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="126"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="195"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="225"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="260"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="DOMON" evidence="13">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b561" evidence="14">
        <location>
            <begin position="84"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="11">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <label>1</label>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="11">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <label>1</label>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="11">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <label>1</label>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="11">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <label>1</label>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00053871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR037471-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAI7757430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="320" mass="34812" checksum="632512424182274D" modified="2024-05-29" version="1">MAGSQALVAFQQSDGSMRVYTSPIKDYTTQLAEGKLSFPVSDLSATYSNNEIIIFATLGLQNGTGTTMNQVWQEGQLSGNAPTAHATSGDNIRSMGTLNVLSGQSGSAGGASGVSSKTKKRNIHGLLNAISWGILMPLGAIIARYLRVFPSADPAWFYLHVTCQTSAYIIGVSGWATGIRLGSQSPGIQFTGHRVIGIILFCVATLQVIALLVRPKKEHKHRIFWNIYHHSLGYSIIILGIINIFKGFDILNPEKKWERAYTAIIIILAIVAAVLEAYTWFVVLKRKKAANTEKMTNGNGYATNGTNEHYPYRGMTNGRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DIQ6</accession>
    <name>A0AAD5DIQ6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_014662</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000008">
        <property type="protein sequence ID" value="KAI7758300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DIQ6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00265">
        <property type="entry name" value="MADS_MEF2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1810.10">
        <property type="entry name" value="Transcription factor, MADS-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050142">
        <property type="entry name" value="MADS-box/MEF2_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033896">
        <property type="entry name" value="MEF2-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002487">
        <property type="entry name" value="TF_Kbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002100">
        <property type="entry name" value="TF_MADSbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036879">
        <property type="entry name" value="TF_MADSbox_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48019">
        <property type="entry name" value="SERUM RESPONSE FACTOR HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01486">
        <property type="entry name" value="K-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00319">
        <property type="entry name" value="SRF-TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00404">
        <property type="entry name" value="MADSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00432">
        <property type="entry name" value="MADS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55455">
        <property type="entry name" value="SRF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51297">
        <property type="entry name" value="K_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00350">
        <property type="entry name" value="MADS_BOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50066">
        <property type="entry name" value="MADS_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MADS-box" evidence="7">
        <location>
            <begin position="29"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="K-box" evidence="8">
        <location>
            <begin position="112"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7758300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="260" mass="29034" checksum="62B17D319283E03C" modified="2024-05-29" version="1">MCGSSSSSSSSSGGGGPGGGGGERKRKTMGRGRIEIKKIENVSSRQVTFSKRRAGLMKKAKELAILCDAEVGVIIFSNTGKLYDFASSRLQNIISRYERTSNGWNDPISGPRQEVHNEVEALKAEILKLRKGQRLMLGKELDGLNYKELENLERQLHDGMLAVKNRKDMALLEEIEQIKLREQKTMKENEALKKEITKHLYNSTSGHEVCLVGRKSPFIKPNTLAYLSPDNGEVGISLHLGLSQRDDHQKRKVPKLEYDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G1R2</accession>
    <name>A0AAD5G1R2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">POX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_010628</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011890">
        <property type="protein sequence ID" value="KAI7725634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G1R2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006563">
        <property type="entry name" value="POX_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07526">
        <property type="entry name" value="POX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="POX" evidence="2">
        <location>
            <begin position="206"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="232"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="234"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="250"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7725634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="263" mass="29094" checksum="803A4DCEA5311B2B" modified="2024-05-29" version="1">MFLLDDLRALPVLFFTLDIPVNIPWHAKVVNSVNLIERVKIPVQFEASSDERLEEMSSYIRSSENGRDSAPMLYLREPLQQSYSSEPTIMAGNEMMYMNYSSASAVEFSGTLAGNSHQLNSCIDIPNIGVHDSSVVSHQELLSSIGEPWRDGRNHGMMLMGQMGSQNLQEPGQTQGNPSMGLSSFISADSKSIKSPYGMGNVSRAITDSKYLKAAQQLLDEIVNVGKALKQHNARKESMDCSKEIDSELKNVTSSNQQELNEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G321</accession>
    <name>A0AAD5G321_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Fatty acid hydroxylase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_032357</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sterol desaturase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011468">
        <property type="protein sequence ID" value="KAI7727014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G321"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006694">
        <property type="entry name" value="Fatty_acid_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050307">
        <property type="entry name" value="Sterol_Desaturase_Related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11863">
        <property type="entry name" value="STEROL DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04116">
        <property type="entry name" value="FA_hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Fatty acid hydroxylase" evidence="6">
        <location>
            <begin position="2"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7727014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="82" mass="9705" checksum="980D49920C7E2EED" modified="2024-05-29" version="1">MVVRVLETVEAHCGYHFPWSPSNFLPLYGGSDFHDYHHRLIYTKSGNYSSTFVYMDWLFGTDTGYRKLKALKSEEEDKYKEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G368</accession>
    <name>A0AAD5G368_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">SCP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_014191</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CRISP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011809">
        <property type="protein sequence ID" value="KAI7725953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G368"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098542">
        <property type="term" value="P:defense response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05381">
        <property type="entry name" value="CAP_PR-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.33.10:FF:000006">
        <property type="entry name" value="Putative pathogenesis-related protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018244">
        <property type="entry name" value="Allrgn_V5/Tpx1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001283">
        <property type="entry name" value="CRISP-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10334">
        <property type="entry name" value="CYSTEINE-RICH SECRETORY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00837">
        <property type="entry name" value="V5TPXLIKE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00198">
        <property type="entry name" value="SCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="PR-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01009">
        <property type="entry name" value="CRISP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01010">
        <property type="entry name" value="CRISP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0568">Pathogenesis-related protein</keyword>
    <keyword evidence="3" id="KW-0611">Plant defense</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041940600" description="SCP domain-containing protein" evidence="6">
        <location>
            <begin position="25"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="SCP" evidence="7">
        <location>
            <begin position="26"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7725953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="161" mass="17797" checksum="8BC1F202EA117E89" modified="2024-05-29" version="1">MGSLKLSLPIICFLTLVMFHNSHAQNSQQDYLNAHNADREQVGVGNMVWNATVATYAQNYANQRIGDCNLVHSGGPYGENLAKGSGTFTGTSAVNLWVAEKLNYDYTTNTCASGYVCGHYTQVVWRNSVQLGCARVQCTNGWWYVICSYYPPGNYIGQSPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G416</accession>
    <name>A0AAD5G416_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">tRNA 4-demethylwyosine synthase (AdoMet-dependent)</fullName>
            <ecNumber evidence="4">4.1.3.44</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">M8C21_010783</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">AA19_3_7</strain>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>N(1)-methylguanosine(37) in tRNA(Phe) + pyruvate + S-adenosyl-L-methionine = 4-demethylwyosine(37) in tRNA(Phe) + 5'-deoxyadenosine + L-methionine + CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:36347"/>
            <dbReference type="Rhea" id="RHEA-COMP:10164"/>
            <dbReference type="Rhea" id="RHEA-COMP:10165"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64315"/>
            <dbReference type="ChEBI" id="CHEBI:73542"/>
            <dbReference type="EC" id="4.1.3.44"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">tRNA modification; wybutosine-tRNA(Phe) biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TYW1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.44" evidence="4"/>
    <dbReference type="EMBL" id="JAMZMK010011533">
        <property type="protein sequence ID" value="KAI7726791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G416"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102521">
        <property type="term" value="F:tRNA-4-demethylwyosine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031591">
        <property type="term" value="P:wybutosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000196">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013917">
        <property type="entry name" value="tRNA_wybutosine-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034556">
        <property type="entry name" value="tRNA_wybutosine-synthase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13930">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE-DEPENDENT TRNA 4-DEMETHYLWYOSINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13930:SF0">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE-DEPENDENT TRNA 4-DEMETHYLWYOSINE SYNTHASE TYW1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08608">
        <property type="entry name" value="Wyosine_form"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00284">
        <property type="entry name" value="tRNA_wybutosine-synthesizing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01071">
        <property type="entry name" value="tRNA_wybutosine-synthesizing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="11" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="12" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="15" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0819">tRNA processing</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042286064" description="tRNA 4-demethylwyosine synthase (AdoMet-dependent)" evidence="15">
        <location>
            <begin position="18"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="Flavodoxin-like" evidence="16">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="17">
        <location>
            <begin position="190"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="84"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="90"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAI7726791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="481" mass="54197" checksum="EAF3D65280FDD06E" modified="2024-05-29" version="1">MILFFFLFISLRFAVFGVGSRAYGDGLFNKVGRGFSAKMRALGAVEIVGLGEGDVDEGELDREFGVWSEEVVRVLKSGELKANESSLVDDGSDYEVDDEEEDYEDEEEETETEIVDLEDIAGKSVRKKDMGMVESNGKKNGEKVMVTPVIRASLTKQGYKIIGSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWKMDDPLMIVNTAIEEHKKMIKQMKGVPGVTAERLSEGLLPRHCALSLVGEPIMYPEINSLIDELHNRRISTFLVTNAQFPDKIVSLNPITQLYVSVDAATKDNMKAIDRPLFGDFWERFVDSLKALKDKQQRTVYRLTLVKGWNTEDIDAYSSLFSIGNPDFVEIKGVTYCGTSATSKLTMENVPWHADVKAFSEALAEKSNGEYEVACEHVHSCCVLLAKVDKFKINGQWHTWIDYEKFHDLVCGIGKAFWKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G5M4</accession>
    <name>A0AAD5G5M4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_015704</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010011037">
        <property type="protein sequence ID" value="KAI7729222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G5M4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd14016">
        <property type="entry name" value="STKc_CK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000236">
        <property type="entry name" value="Casein kinase 1-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055900">
        <property type="entry name" value="DUF7477"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24289">
        <property type="entry name" value="DUF7477"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="33"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7729222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="594" mass="66868" checksum="A11AAAF0B4CF2E98" modified="2024-05-29" version="1">MEGGSADRIIGVEEEGNATPVPDRVQVGNSPIYKTERKLGKGGFGQVYVGRRVSGGSGRTGPDAVEVALKFEHRNSKGCNYGPPYEWQVYSSLNGCYGIPWVHYKGRQGDFYILVMDMLGPSLWDVWNSLGQSMAPNMVACIAVEAISILEKLHSKGFVHGDVKPENFLLGQPGSVDEKKLYLIDLGLASKWRESSSNQHVEYDQRPDIFRGTIRYASVHAHLGRTGSRRDDLESLAYTLIFLIKGRLPWQGYQGDNKSFLVCKKKMATSPELMCCFCPAPFKQFLEAVTNMKFDEEPNYAKLISFFDTLIEPVTSLRPIRIDGALKVGQKRGRLLINLEDDEQPKKKVRVGSPASQWVSVYNARRPMKQRYHYNVADLRLSQHVEKGNEDGLYISCVASASNLWALIMDAGTGFCSQVYELSSVFLNKDWIMEQWEKNYYISSIAGAANGSSLVVMSKGTPYTQQSYKVSESFPFKWISKKWKEGFHVTSMTTAGSRWGVVMSRNSGFSDQVVELDFLYPSEGIHRRWENGYRITSMAATPDQAAFILSIPRRKVMDETQETLRTSAFPSTHVKEKWSKNLYIAAICYGRTVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G5X0</accession>
    <name>A0AAD5G5X0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PLAC8 motif-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_028662</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011181">
        <property type="protein sequence ID" value="KAI7728623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G5X0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006461">
        <property type="entry name" value="PLAC_motif_containing"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01571">
        <property type="entry name" value="A_thal_Cys_rich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15907">
        <property type="entry name" value="DUF614 FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04749">
        <property type="entry name" value="PLAC8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="107"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="22"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="43"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7728623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="203" mass="21955" checksum="4945C9CEF729CF00" modified="2024-05-29" version="1">MNSSSPKDNKDKAPQPTNDAQPATAAPPSTASESDSGKAEKGPSSQQPSEQPVVPVPPQLVMYPTWSTGLFECCDDIPTLLITCFAPCVTFGQVAEMVDRGQNSCGLYAMLHAGILYLTGCGCLLSAYYRIKMTQLYNLPNDPLINILVHLICEPCALCQEYRELQARGFNMKLGVGWRNQSLEIQQTGGLMVAPKVPGGMTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G692</accession>
    <name>A0AAD5G692_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_028725</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010876">
        <property type="protein sequence ID" value="KAI7729957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G692"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7729957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="212" mass="23361" checksum="0E763FB7418BE6C3" modified="2024-05-29" version="1">MVAFRWKYQTVAAAVMVVTNQKSRHSFQTLIQESEDGKSHDDVLLLRVSAVEAKEHQIGSLFQVNSLSSGGGGLALPCPDFNTLLMSVTRSINPDKFGFVVWYSDKHEGKMVIGAIFGVLNPVSGQSSSVSASRFLLFFQLSKNSFIFGALTRADNKATLPALFVALPVLSPKKKWVMLYSTWRHDMSLPILYRKVDGAPKGEVFGGLVRHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G6F9</accession>
    <name>A0AAD5G6F9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_019441</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011071">
        <property type="protein sequence ID" value="KAI7729103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G6F9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7729103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="163" mass="17886" checksum="8D45B3C07BD6A8F9" modified="2024-05-29" version="1">MYTPRKEEDAGERATNITWNKGMADVKRKQGEILIAGAYLTVERRSLFIIVVAAAAAAAALEIKHGYKKVASASTSSSMFLSFQKLEGYLAQQWSPLHELGKDSVFVATSDNLGVVVGRWWHLMVDKPVLYDLGDGVIHITLGSSFFTSQGSLLSSSVYFDNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G7F5</accession>
    <name>A0AAD5G7F5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Amidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_032239</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010364">
        <property type="protein sequence ID" value="KAI7731875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G7F5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000120">
        <property type="entry name" value="Amidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895:SF73">
        <property type="entry name" value="AMIDASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895">
        <property type="entry name" value="TRANSAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidase" evidence="2">
        <location>
            <begin position="131"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="385"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7731875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="420" mass="47122" checksum="8A86F269D3A3175F" modified="2024-05-29" version="1" fragment="single">DSASPESIRHEKRAKKIGAASGLERGFSMLDAEFFNDSKMLEIEKGAKELTIPIIKDNRKIVAATNGGLKYPSPLVFNSEWKHEEIHDDVKRFNYPSVSSVKRPENEEDIAFMTVLELGHLIRTKQISSEELVGIFLKRLKRYNQVLEAVITVTEDLAYRQAKQADQLLAQGVDLGPLHGIPYGLKDIIAVPEYKTTWGSTTFKDQVLDIEAWVYKRLKSAGAVLVGKLVTGSLAYDDIWFGGILNFTMDVDMLAHFDTWQRSGQDDEFEAQDQWPTELRRARVIPAVDYIQSQRARGKLIQEVKESFTVDAFVGNATDWEKVCVGNLVGMPVMVVPVGFEKIKDPPTNGTRRRSTVTTGIYAPPDHDHIALALTMAYQSVTNHHKRRPPIDNLGPDDTIRNPPKTVIPPRILKAMVDAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G7V5</accession>
    <name>A0AAD5G7V5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">isopentenyl-diphosphate Delta-isomerase</fullName>
            <ecNumber evidence="5">5.3.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_021961</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the 1,3-allylic rearrangement of the homoallylic substrate isopentenyl (IPP) to its highly electrophilic allylic isomer, dimethylallyl diphosphate (DMAPP).</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from isopentenyl diphosphate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Porphyrin-containing compound metabolism; chlorophyll biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the IPP isomerase type 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.3.2" evidence="5"/>
    <dbReference type="EMBL" id="JAMZMK010010766">
        <property type="protein sequence ID" value="KAI7730498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G7V5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004452">
        <property type="term" value="F:isopentenyl-diphosphate delta-isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015995">
        <property type="term" value="P:chlorophyll biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009240">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02885">
        <property type="entry name" value="NUDIX_IPP_Isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.79.10:FF:000025">
        <property type="entry name" value="isopentenyl-diphosphate Delta-isomerase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011876">
        <property type="entry name" value="IsopentenylPP_isomerase_typ1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02150">
        <property type="entry name" value="IPP_isom_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10885">
        <property type="entry name" value="ISOPENTENYL-DIPHOSPHATE DELTA-ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10885:SF12">
        <property type="entry name" value="ISOPENTENYL-DIPHOSPHATE DELTA-ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0149">Chlorophyll biosynthesis</keyword>
    <keyword evidence="8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="9">
        <location>
            <begin position="112"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7730498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="294" mass="33303" checksum="10C716D9A6FCE7C4" modified="2024-05-29" version="1">MAASLISRCSIHHSKSHKLSYPLSLSSTSSPPQFISFKSRLPVFSFTAGVSSSSSHVTSPVLGGDADSTMDAVQRRLMFEDECILVDANDGVVGHDTKYNCHLMEKIESENLLHRAFSVFLFNSKYELLLQQRSSTKVTFPLVWTNTCCSHPLYRESELIEENCLGVRNAAQRKLLDELGIPSEELPVDEFIPLSRILYKAPSDGKWGEHELDYLLFLVRDVSMNPNPDEVADVKYVNREQLRELVRKADAGEEGLKLSPWFRIVVDNFLFKWWDHAEKGSLHEAADMKTIHSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GB28</accession>
    <name>A0AAD5GB28_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">50S ribosomal protein L18</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_011186</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009404">
        <property type="protein sequence ID" value="KAI7735940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GB28"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008097">
        <property type="term" value="F:5S rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00432">
        <property type="entry name" value="Ribosomal_L18_L5e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057268">
        <property type="entry name" value="Ribosomal_L18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005484">
        <property type="entry name" value="Ribosomal_uL18_bac/plant/anim"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12899">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L18, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12899:SF6">
        <property type="entry name" value="OS01G0256600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00861">
        <property type="entry name" value="Ribosomal_L18p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7735940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="118" mass="13040" checksum="B8DD9452010240BA" modified="2024-05-29" version="1">MVIPPPVKTQRVTNYLKPYLLKMHFSNKYVSAQVIHAPTATVASAASSQEKALCEAWTKTQQSTRDVAAAAKIGKLLGERLLHKDIPAVSVVYRKDQRYHGKVRAVIDSLREAGVTLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GCA8</accession>
    <name>A0AAD5GCA8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">CASP-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_027310</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3 8">Homodimer and heterodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Casparian strip membrane proteins (CASP) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009119">
        <property type="protein sequence ID" value="KAI7737080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GCA8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006459">
        <property type="entry name" value="CASP/CASPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006702">
        <property type="entry name" value="CASP_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01569">
        <property type="entry name" value="A_tha_TIGR01569"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33573:SF46">
        <property type="entry name" value="CASP-LIKE PROTEIN 2A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33573">
        <property type="entry name" value="CASP-LIKE PROTEIN 4A4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04535">
        <property type="entry name" value="CASP_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="35"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="73"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="115"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="154"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="Casparian strip membrane protein" evidence="9">
        <location>
            <begin position="27"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7737080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="201" mass="21988" checksum="3E274E4C93D55446" modified="2024-05-29" version="1">MANNNAKTTDVATRIATTCQEEDGSSSGIRMTEMLLRLIPVGLCVAALVVMVKDSESNDYGSLSYSNFTGFRFLVHANGILAGYSLVSAAFTSVPRPMTMPRAWTFFLLDQVSTYLILAAGAVATELVFLAYKGDEQVTWSEACGTYGHFCRQATTSIIITFLVVVCYVLLSLISSYRLFSKYDAPVNKEIEIVDLGNQEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GDV2</accession>
    <name>A0AAD5GDV2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_009811</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008774">
        <property type="protein sequence ID" value="KAI7738625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GDV2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22162">
        <property type="entry name" value="F-box_AtSKIP3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025886">
        <property type="entry name" value="PP2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31960:SF22">
        <property type="entry name" value="F-BOX PROTEIN PP2-A12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31960">
        <property type="entry name" value="F-BOX PROTEIN PP2-A15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14299">
        <property type="entry name" value="PP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="24"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7738625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="272" mass="30570" checksum="3140B732DE5F934B" modified="2024-05-29" version="1" fragment="single">MGSGFSSFLNIYNSTSSVSSVSAETGLGDLPESVVGSVLMHLNPQEICQVASINRAFRGASSADFVWESKLPENYCSVFKWIFNDDDDKLSDLCKKDVYARLSRLNSIDGDTKKVWLHKGTGKSCVLVSFNGLTVTGIDDRRYWSRISTEESRFGSVAYLQQTWWFEVDGEVEFPFPVGTYSLYFRLQLGRPGKQFGRRVCNREHIHGWDIKPVRFQLATTDGQQAISQCYLTTPGTWNLYHAGSFVVEDSKSPTKVKFSMMQIDCTHTKGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GGG6</accession>
    <name>A0AAD5GGG6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">BUB1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_012486</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008436">
        <property type="protein sequence ID" value="KAI7740424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GGG6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051754">
        <property type="term" value="P:meiotic sister chromatid cohesion, centromeric"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007094">
        <property type="term" value="P:mitotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015661">
        <property type="entry name" value="Bub1/Mad3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013212">
        <property type="entry name" value="Mad3/Bub1_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14030:SF4">
        <property type="entry name" value="BUB1 KINASE, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14030">
        <property type="entry name" value="MITOTIC CHECKPOINT SERINE/THREONINE-PROTEIN KINASE BUB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08311">
        <property type="entry name" value="Mad3_BUB1_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00777">
        <property type="entry name" value="Mad3_BUB1_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51489">
        <property type="entry name" value="BUB1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BUB1 N-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7740424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="204" mass="23480" checksum="723A92EC41D7B015" modified="2024-05-29" version="1" fragment="single">MMKQMAVDDATMDDPLLPHLWSIKGALDKLDLWKKQNEEKQEEERNVYELVSQCIQSFKHHPLYRNDIRFLKIWLLYMDLSGDHAAVFNEIQLLNICLDKALLYESYALLLEAMGLMSQANAVYQSGISRNAQPFGRLNNAHQSFLHRMHTIVAACQQLPNMDGENGYVNPWSISVIKNLLQKMNAQLSKYQGYHATSKSYSGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GHD1</accession>
    <name>A0AAD5GHD1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Serine/threonine-protein kinase 19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_029858</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the STK19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007933">
        <property type="protein sequence ID" value="KAI7742690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GHD1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018865">
        <property type="entry name" value="STK19-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15243">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15243:SF0">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10494">
        <property type="entry name" value="Stk19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="69"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="76"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00093458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7742690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="333" mass="38164" checksum="75ABA85C6AF87ABB" modified="2024-05-29" version="1" fragment="single">NKHTFQTKNFIQKYISLYGTRNLAFVSTVIGYPTVAVAGDRKTTNFAAGDRILIPNQVFLKPYPFGSESDPKNLHSMETNNTPSTSRKRVREDDDSVPESNHSLSLEDDLIFSDTMVALQMMCAQFPRIDKVTVEPFVLQSQLYSSVKDRTQVDRELESLRREKVLRVFKLNTGQDDHALMFMEDYLKQMKRAVKSMETKKQGVFPVFEWFEVHVIHSKPEPCIGHQELLSLLSVGGKVKDEDISLLINAGLLTRQLIDSEMYWFAIPNIGSVLKGITQGRKELLSLLNRKKYKEMMLAILEKKRLRLSPLDMRFHLRDLLGSGHLKTIQTPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GHG2</accession>
    <name>A0AAD5GHG2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Proteasome subunit alpha type</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_022130</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the peptidase T1A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008527">
        <property type="protein sequence ID" value="KAI7740068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GHG2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03755">
        <property type="entry name" value="proteasome_alpha_type_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000004">
        <property type="entry name" value="Proteasome subunit alpha type-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003075">
        <property type="entry name" value="PRK03996.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10584">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00388">
        <property type="entry name" value="PROTEASOME_ALPHA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2 3 4" id="KW-0647">Proteasome</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome alpha-type subunits" evidence="5">
        <location>
            <begin position="4"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS00388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7740068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="248" mass="27064" checksum="D5390217AF320F76" modified="2024-05-29" version="1">MARYDRAITVFSPDGHLFQVEYALEAVRKGNAAVGVRGTDTIVLGVEKKSTVKLQDSRSVRKIVTLDDHIALACAGLKADARVLINRARIECQSHRLTVEDPVTVEYITRYIAGLQQKYTQSGGVRPFGLSTLIVGFDPYTGVPALYQTDPSGTFSAWKANATGRNSNSMREFLEKNYKETSGQETVKLAIRALLEVVESGGKNIEVGVMTKEGLKQLEEDEIDTIVAEIEAEKAAAEAAKKAPSKQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GHQ5</accession>
    <name>A0AAD5GHQ5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">glutathione transferase</fullName>
            <ecNumber evidence="2">2.5.1.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">GST class-phi</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_030244</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GST superfamily. Phi family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010007851">
        <property type="protein sequence ID" value="KAI7742962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GHQ5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043295">
        <property type="term" value="F:glutathione binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009407">
        <property type="term" value="P:toxin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03187">
        <property type="entry name" value="GST_C_Phi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03053">
        <property type="entry name" value="GST_N_Phi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000004">
        <property type="entry name" value="Glutathione S-transferase F2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000016">
        <property type="entry name" value="Glutathione S-transferase F2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034347">
        <property type="entry name" value="GST_Phi_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43900">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43900:SF65">
        <property type="entry name" value="GLUTATHIONE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="6">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="7">
        <location>
            <begin position="88"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00081070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7742962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="212" mass="24368" checksum="B4AF72351F065C51" modified="2024-05-29" version="1">MVVKVYGPAYASPKRVLLCLVEKQIEFETVPVDLIAGENHNPEFLKLQPFGSLPVIQDGDYTLFESRAIIRYYAEKYKSQGTDLLGKTLEDQGLVNQWLEIEAHNYHPPLLNLVLHIMFSPHFGIVRNEQVIKESEEKLAKVLDIYEDRLSKSKYLAGDFFSLADLSHIPFTNYLVGPVGKGYMIKERKHVSAWWDDISSRPSWLKVLEICK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GI66</accession>
    <name>A0AAD5GI66_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MO25-like protein At5g47540</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_001206</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Mo25 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008349">
        <property type="protein sequence ID" value="KAI7740773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GI66"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043539">
        <property type="term" value="F:protein serine/threonine kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013878">
        <property type="entry name" value="Mo25"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10182:SF29">
        <property type="entry name" value="ARMADILLO-LIKE HELICAL, MO25-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10182">
        <property type="entry name" value="CALCIUM-BINDING PROTEIN 39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08569">
        <property type="entry name" value="Mo25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7740773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="346" mass="39560" checksum="A06BD52E7F140AC9" modified="2024-05-29" version="1">MLCLSSLGKTVMNTKKLRTPAEVVQKTRSLLLYSKINSLDVGSQMGELNSLIRELKLILYGDDDSEPSAEACAQLTVEFFKEDTLRLLIIFLPKLNLEARKDATQVVASLQRQPLPSRFEVSRYLEANLDLLDILISGYEDPQLALHYGRMLKECLRHQIVASYLLEPIQLEKLFGYIQHPSFDIAADAADAFKDLLTRHKSTVSESLSKNYVWVDIPYFFTEMNEKLLKSGNYITRRQAVKLLGCILLDRSNSCVMTRYVSSKDNLIILMNLLRDQSRNIQIDAFHVFKLFVANENKPPEIVSILVTNRSKILRLLSAFAYTDDEMFETDKIQVINKLAVLELQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GI87</accession>
    <name>A0AAD5GI87_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Photosystem I reaction center subunit IV B, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_000791</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Stabilizes the interaction between PsaC and the PSI core, assists the docking of the ferredoxin to PSI and interacts with ferredoxin-NADP oxidoreductase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Plastid</location>
            <location evidence="2">Chloroplast thylakoid membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PsaE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007713">
        <property type="protein sequence ID" value="KAI7743592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GI87"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009538">
        <property type="term" value="C:photosystem I reaction center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015979">
        <property type="term" value="P:photosynthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.50:FF:000001">
        <property type="entry name" value="Photosystem I reaction center subunit IV, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008990">
        <property type="entry name" value="Elect_transpt_acc-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003375">
        <property type="entry name" value="PSI_PsaE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34549:SF9">
        <property type="entry name" value="PHOTOSYSTEM I PSAE, REACTION CENTRE SUBUNIT IV-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34549">
        <property type="entry name" value="PHOTOSYSTEM I REACTION CENTER SUBUNIT IV A, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02427">
        <property type="entry name" value="PSI_PsaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50090">
        <property type="entry name" value="Electron transport accessory proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0602">Photosynthesis</keyword>
    <keyword evidence="7" id="KW-0603">Photosystem I</keyword>
    <keyword evidence="6" id="KW-0934">Plastid</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0793">Thylakoid</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="56"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7743592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="136" mass="14456" checksum="0AABC678085F0AB5" modified="2024-05-29" version="1">MASCSMASAAVGFVVAPNVASNTSTVKCTMVMFSSKKIPRSSRLVVKAADEAAAAPPAAAAEPAKEAAPKPPPIGPKRGAKVRILRKESYWYKGVGSVVTVDQDPKTRYPVVVRFNKVNYANVSTNNYALDEIQEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GIS4</accession>
    <name>A0AAD5GIS4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein GAMETE EXPRESSED 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_020795</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007572">
        <property type="protein sequence ID" value="KAI7744177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GIS4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009793">
        <property type="term" value="P:embryo development ending in seed dormancy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010183">
        <property type="term" value="P:pollen tube guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045301">
        <property type="entry name" value="GEX3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37253">
        <property type="entry name" value="PROTEIN GAMETE EXPRESSED 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37253:SF1">
        <property type="entry name" value="PROTEIN GAMETE EXPRESSED 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042156509" description="Protein GAMETE EXPRESSED 3" evidence="2">
        <location>
            <begin position="24"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="516"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="604"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="516"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="544"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="566"/>
            <end position="585"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7744177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="626" mass="69644" checksum="1F03C15D77812E90" modified="2024-05-29" version="1">MSQIIKILTQILTLNMIQLISVASDDPKLHTYQHWITGPPRIPKPNTNISNPLITDEGKIYTCLDKTILVFNNNGSLINRISLNHTCNPGMTPVLGASRKIYMIAENRVLKINTMKIQSNEPAWEVFLGPETGVQGLNKIIGLAVSISNSYVIVTIERRGLFGYRFDGRLKWSVGPAINRGGYRQVPVIDQCDANIYVANNQGELYSVSIRSRHFKWIQDLSWLDRNFTITAGNNGRLYATVQERSLIVALDVVTGTVLWQQIIGPLHTQDSEPVADNNGWISMGSLDGFLYSISPSGIVNKFPQRKNLDTVVQQPVLDCSGYAVYVSQTKFDGKISRSTDENTYVSAMTPLKTVFTLLVPATGSVIWSENYPGGSVVSSLFPESDLRHFMVDEGMLLAFLSISKTGNPLSCFSTRQKLASSCSIMDIKKVSIYTALVRFCCVFWKKKKLQNQNLGNFLEKRCSLRLQKKVCDRTITELEKKTAEGSSTNEMIEKLGDLVKEREVIERKLSTTYSLGRDATSPQSESLIPLSDKKTKSFSPKSGQKDSADIFHSVDDTSSEDYSSNEEKTESKGKALMEPERTSDDGFDDEAIVEAQGVMIVDQGDDLQTGGSSMRKRSISLNRNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GLA3</accession>
    <name>A0AAD5GLA3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Amino acid transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_033326</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006825">
        <property type="protein sequence ID" value="KAI7747147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GLA3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF696">
        <property type="entry name" value="AMINO ACID TRANSPORTER TRANSMEMBRANE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="81"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="106"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="152"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="191"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="225"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="261"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="294"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="338"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="381"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="406"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="437"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="7">
        <location>
            <begin position="74"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7747147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="473" mass="52224" checksum="4A878E1B96A37C1C" modified="2024-05-29" version="1" fragment="single">FWRQKNVDCSNEQTENKSTHEANSTLKWVGCTETCLEGGKESCQCGNENIDRDELRFEVKNKSSDEQASPHEAGSSFLHSVANMIGMLIGLGQLSTPYALENGGWISSFLLIGLGIATAYTSHLLGKCLETNRKTRNYTDIGYHAFGTHGRAITAAFIYLEIFMSLVSYTISLHDNLNMVFLGTHVRFSWVHHLSTSQVLTVLAVLVALPSLWLRDLSSISFLSIGGIVMSMMIFVTVVCTAVFGGVAANRDIPALRLRNIPHISGLYVFGYGGHIVFPDIHRAMKDPSKFTKVSIVSFTFVTLLYTSLAFMGAKLFGPEVNSQITLSMPRGLVFTKIALWATVLTPMTKYALEFAPFAIQLEHRFLYSMKSQTKMIIRGTIGSVLLLLILVLALTVPYFQYVLGLTGSLVTVAIILIFPSIFYIKIFWDKISRPVLALNVFLIAIGFLLGVSGTISSLKLLVGSFERIHSTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GMF5</accession>
    <name>A0AAD5GMF5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">MSP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_007564</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the VAMP-associated protein (VAP) (TC 9.B.17) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006526">
        <property type="protein sequence ID" value="KAI7748407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GMF5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090158">
        <property type="term" value="P:endoplasmic reticulum membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061817">
        <property type="term" value="P:endoplasmic reticulum-plasma membrane tethering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000535">
        <property type="entry name" value="MSP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008962">
        <property type="entry name" value="PapD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016763">
        <property type="entry name" value="VAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10809:SF110">
        <property type="entry name" value="MSP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10809">
        <property type="entry name" value="VESICLE-ASSOCIATED MEMBRANE PROTEIN-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00635">
        <property type="entry name" value="Motile_Sperm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49354">
        <property type="entry name" value="PapD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50202">
        <property type="entry name" value="MSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MSP" evidence="3">
        <location>
            <begin position="77"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="7"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="23"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="53"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7748407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="269" mass="30746" checksum="DE9B7E68491E5D41" modified="2024-05-29" version="1">MSIADHHRSHSNGKNRRLFPFWQSGTTTSSSKQNHQLQFHHHHNNNNSSNENGVVSTARSSNKLSSVAKSLMPARRRLRLDPGNNLYFPYEPGKQVRSAVRIKNHSSSHVAFKFQTTAPKSCYMRPPGGVLAPGESLIATVFKFVEQPDKNEKQLDQRSKDCKVKFKIMSLKVKEGTDFVPELFDEQKDEVVVERILRVIFLDADRPTPALEKLKHQLAEAEAQLELRRKPQVETGPKVVGQGLVIDEWKERREKYLARQQEVEAVDLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GMQ1</accession>
    <name>A0AAD5GMQ1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Peptidase M50 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_011747</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M50B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006513">
        <property type="protein sequence ID" value="KAI7748510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GMQ1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031969">
        <property type="term" value="C:chloroplast membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044838">
        <property type="entry name" value="EGY1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008915">
        <property type="entry name" value="Peptidase_M50"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31412">
        <property type="entry name" value="ZINC METALLOPROTEASE EGY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31412:SF0">
        <property type="entry name" value="ZINC METALLOPROTEASE EGY1, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02163">
        <property type="entry name" value="Peptidase_M50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4" id="KW-0934">Plastid</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Peptidase M50" evidence="12">
        <location>
            <begin position="18"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7748510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="92" mass="9843" checksum="6F6FDB19261CE181" modified="2024-05-29" version="1" fragment="single">NKKDIYSTLEYTVMHRATISIHPLVIAVWCGLTTTAFNMLPVGCVDGGRVVQGAFGKYALVGFGLTTYSLLGLGVHGGPLSLPWGLYVLICQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GMZ3</accession>
    <name>A0AAD5GMZ3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tyrosine specific protein phosphatases domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_026620</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007038">
        <property type="protein sequence ID" value="KAI7746126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GMZ3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14527">
        <property type="entry name" value="DSP_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47216">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47216:SF4">
        <property type="entry name" value="OS01G0859400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="1">
        <location>
            <begin position="41"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7746126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="114" mass="12627" checksum="CF08C11608BB4767" modified="2024-05-29" version="1" fragment="single">MPPGNPAVVDCTCELPRVFRVSGGNGYLCVPVWDTRAPDPGAIENAVRWACRKRSQNVPVFVHCAYGHGRSVAITCALLVALGVVDDWKNAEKLIKDKRPYIRMNALHRQALEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GNN8</accession>
    <name>A0AAD5GNN8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_030492</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006352">
        <property type="protein sequence ID" value="KAI7749485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GNN8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05243">
        <property type="entry name" value="SDR_a5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15020">
        <property type="entry name" value="FLAVIN REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15020:SF11">
        <property type="entry name" value="OS06G0360300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13460">
        <property type="entry name" value="NAD_binding_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="300"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="NAD(P)-binding" evidence="2">
        <location>
            <begin position="60"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7749485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="336" mass="36843" checksum="524A9F5A7476C940" modified="2024-05-29" version="1">MWPITMAASSPLLLLRSFTPTKPFSKTILSLRKPLKFYSNSIRMEISEIEAEKKKIFVSGATGKTGKRIVEQLLAKGFAVKAGVRDAEKAKSMFPNPNEDLQIIKSDVTEGSEKLANAIGDDSDAVICATGFQYSWDLLAPWKVDYYGTVNLVDACRKVGVKRFILVSSILVNGAAMGQLLNPAYVFLNAFGLVLVAKLQAEQYIRKSGINYTIIRPGGLKNDPPSGNIVMEPEDTLSEGSISRDQVAEVAVESLLHPQASFKVVEIVARTEAPSKSFVELFGSIKERSRLTHVLNSLKLIALVLVFSIALMALQQLKILKVLIGMTRRLNEDVGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GQ18</accession>
    <name>A0AAD5GQ18_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Leucine-rich repeat-containing N-terminal plant-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_027979</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006552">
        <property type="protein sequence ID" value="KAI7748331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GQ18"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000024">
        <property type="entry name" value="Somatic embryogenesis receptor kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47988">
        <property type="entry name" value="SOMATIC EMBRYOGENESIS RECEPTOR KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042263075" description="Leucine-rich repeat-containing N-terminal plant-type domain-containing protein" evidence="4">
        <location>
            <begin position="24"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="5">
        <location>
            <begin position="24"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="6">
        <location>
            <begin position="71"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7748331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="214" mass="23426" checksum="3E158565B143B2F9" modified="2024-05-29" version="1">MTTMAWGALIITVLVASSALVTGNSEGDALYALCRSLNDPDNVLQSWDPNLVNPCTWFHITCNQDNRVTRLDLGNSKLSGHLVPELGKLEHLQYLELYKNTIQGPIPAEIGNLKNLISLDLYNNNISGSIPPTLGKLKSLVFLRLNDNKLTGRIPRELTGISSLKVMDVSNNDLCGTIPTTGPFEHIPLNNFENNPRLEGPELLGLASYDTNCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GRL0</accession>
    <name>A0AAD5GRL0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein DETOXIFICATION</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Multidrug and toxic compound extrusion protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_024886</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006253">
        <property type="protein sequence ID" value="KAI7749936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GRL0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13132">
        <property type="entry name" value="MATE_eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045069">
        <property type="entry name" value="MATE_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11206">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="113"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="150"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="284"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="317"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="350"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="377"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="487"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="531"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="560"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="591"/>
            <end position="611"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7749936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="642" mass="71186" checksum="A7A9B0C81078476C" modified="2024-05-29" version="1">MDDGMETKLLRHEEEEEGDLKQRIWVESKKIWRVALPGVISRVCAFGTLIVTQSFIGHISDIDLAGYALVQTLSVRFVNGILLGMSSATETLCGQAFGAGQHHMMGIYLQRSWIVDLITLTILLPVFIFGTQIYKLVGEEESIANSGGYISLWFIPFVYNFKNVTITYSAFRMDDGMETRLIQHEEEEGELTQRIWSESKKIWRVALPGVISRVSAFGTIVVTQSFIGHISDIDLAGYALVQTLSVRFINGILLGMSSATETLCGQAFGAGQHHMMGIYLQRSWIVDSITLTILLPILIFGTQIFKLLGEEEAIANSGGYISLWFITFVYNYVFSLTIQMYLQSQLKNMIIAWLSAIRFVIHIPLSMLFVYKLNMGVAGAMIALSMSSWILVIGEFIYIFGGWCPNSWKGFTVAAFKDLVPVVKLSISSGVMLCLELWYNAVLVLLAGYMENAEVAISAFSICLNLNAWDVRVANELGRGNARAVRFSIQVLLGTSMAIGVFFFVLCLLFGKKLAYLFTDDERVADTVSDLSLLLSFSVLLNSIYPILSGVAIGAGMQGTVAIVNLVCYYLIGIPLGALLGYKTSLQVKGIWIGMIGGVVAQTIALAYMTWRTNWDNEVMKASERLGRFYLKSDESSDQISN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GRV8</accession>
    <name>A0AAD5GRV8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">tRNA pseudouridine synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_019632</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005631">
        <property type="protein sequence ID" value="KAI7752725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GRV8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.660">
        <property type="entry name" value="Pseudouridine synthase I, catalytic domain, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.580">
        <property type="entry name" value="Pseudouridine synthase I, catalytic domain, N-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001406">
        <property type="entry name" value="PsdUridine_synth_TruA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020095">
        <property type="entry name" value="PsdUridine_synth_TruA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020094">
        <property type="entry name" value="TruA/RsuA/RluB/E/F_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11142">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11142:SF10">
        <property type="entry name" value="TRNA PSEUDOURIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7752725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="338" mass="37963" checksum="2BCF2E80591C62BD" modified="2024-05-29" version="1">MDNNINNNKFTPQTKDYLHYNHTDSCSSSRWTSRESYLFMYARPWDKVVNFYSNLVNAHTTLHHLFGAKTSHVLDNSVLEGSDESEYVVTPAQKRAGRWARMNFKIILSYHGGSFDGWQKQPGLNTVQEVIEKSLGKFVDERKAGLLKDKGLPLEGNAVVAGRTDKGVTDTWREDVRPEDIEDAINSTLPGKLRVISVSKVPRTFHPNFSAKWRRYMYIFPLNAGETGEEDATIIYDGKTGDQKNSCNDTIIEENIELANDDDKDDTESIKKPTRFEMVRVLVATSIREAAAGADDDALIKLMDATCRRATAPPAPPDGLCLVDVGYNDFDSQTCLII</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GSY7</accession>
    <name>A0AAD5GSY7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_029087</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005009">
        <property type="protein sequence ID" value="KAI7753955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GSY7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7753955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="119" mass="13341" checksum="9F4552FD9AC3EA6D" modified="2024-05-29" version="1" fragment="single">MASLGTGSLCKPTSLLLSGVVKSSSSLTTGARASKFRRISNFDMNESLTFQRSHCNNHTDQKFYERFTPEKKKNKFGLNATSTNNPQFDATHDLINPLGNVFRFVEVLYRFIRPYAAVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GUJ0</accession>
    <name>A0AAD5GUJ0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">V-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_000129</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005599">
        <property type="protein sequence ID" value="KAI7752846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GUJ0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001388">
        <property type="entry name" value="Synaptobrevin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042855">
        <property type="entry name" value="V_SNARE_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45806">
        <property type="entry name" value="SYNAPTOBREVIN HOMOLOG YKT6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45806:SF1">
        <property type="entry name" value="SYNAPTOBREVIN HOMOLOG YKT6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00957">
        <property type="entry name" value="Synaptobrevin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00219">
        <property type="entry name" value="SYNAPTOBREVN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00417">
        <property type="entry name" value="SYNAPTOBREVIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50892">
        <property type="entry name" value="V_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="V-SNARE coiled-coil homology" evidence="2">
        <location>
            <begin position="56"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7752846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="101" mass="11407" checksum="59CA630636674B63" modified="2024-05-29" version="1" fragment="single">MGSDVAPPNTFKNPSNCLQEETSRLSKNGFKEVGSYLEKKQVQYDMAFLIVVKLFLLLKIQRELDESKFILHKTIDSILERGEKLDSLVEKSSDLSAASQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GUS6</accession>
    <name>A0AAD5GUS6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Putative gamma-glutamylcyclotransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_010514</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Putative gamma-glutamylcyclotransferase.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the gamma-glutamylcyclotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005550">
        <property type="protein sequence ID" value="KAI7753101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GUS6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06661">
        <property type="entry name" value="GGCT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.490.10">
        <property type="entry name" value="Gamma-glutamyl cyclotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045038">
        <property type="entry name" value="AIG2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009288">
        <property type="entry name" value="AIG2-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013024">
        <property type="entry name" value="GGCT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036568">
        <property type="entry name" value="GGCT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31544">
        <property type="entry name" value="AIG2-LIKE PROTEIN D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31544:SF2">
        <property type="entry name" value="AIG2-LIKE PROTEIN D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06094">
        <property type="entry name" value="GGACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110857">
        <property type="entry name" value="Gamma-glutamyl cyclotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Gamma-glutamylcyclotransferase AIG2-like" evidence="6">
        <location>
            <begin position="27"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00030602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF06094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7753101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="187" mass="21383" checksum="56983BAA8D14CB24" modified="2024-05-29" version="1">MSSAISVTAVGNCNHGDNNNNNIIRTVFVYGSLLADDVVRALLRRVPKYSPAILHGYHRFSIKGRVYPAILPVENKKITGRVLMDITAPELDILDKFEDYEYERRLVDVTLRDSSDVLQAYTYVWANSGDPDLYGEWDFEAWKESKMKDFLNMTVEFAEAQELPQSKPRVATYESFYKQNEGNIISS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GW43</accession>
    <name>A0AAD5GW43_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_016491</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="5">Binds 1 Fe(2+) ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the carotenoid oxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000097">
        <property type="protein sequence ID" value="KAI7757600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GW43"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009570">
        <property type="term" value="C:chloroplast stroma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010436">
        <property type="term" value="F:carotenoid dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016121">
        <property type="term" value="P:carotene catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004294">
        <property type="entry name" value="Carotenoid_Oase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10543">
        <property type="entry name" value="BETA-CAROTENE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10543:SF142">
        <property type="entry name" value="OS06G0162550 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03055">
        <property type="entry name" value="RPE65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="4 5" id="KW-0408">Iron</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="5">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="253"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="317"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR604294-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7757600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="330" mass="37273" checksum="62DFD8894C1E9FEB" modified="2024-05-29" version="1">MVDLIFEFVDQPMIPSQKNFASVEEIGESVKVDCCQGNIPEDFPEGVYIRNGSNPLFGGLKLAVSVFGKTDSVWVEGEGMLHALHFKKDEDGEWIFYYKNRYVETDTYKMESQRKKPAFLPVAEGDPLATLVASFFNMLRFGQEYKIMSNTNVFMHGGKHYSIAENYIPQEINPISLETYGNWNPGGAWSRAFTSHPKKVPGTGELVTVGVEAKKPYCIVGIISATNQVDLIADGKELVHRLDLQLDQCSVFHDVGVTNKYTILVDFMLTISPERVMKGGSLFKYEREKDARIAVIPRYGDADSIKWFHIEPCVSYHIMNTFEDGDEVHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GXF9</accession>
    <name>A0AAD5GXF9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_008737</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010001688">
        <property type="protein sequence ID" value="KAI7755188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GXF9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7755188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="63" mass="6742" checksum="B68BD1537553FE8E" modified="2024-05-29" version="1" fragment="single">YDNCLYMTAGSNNNITAASNITANSHANAHSSSSGQRKFPAPSRYCFLLVIVFATIQSLSTNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GYN3</accession>
    <name>A0AAD5GYN3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Telomerase Cajal body protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_011643</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000410">
        <property type="protein sequence ID" value="KAI7756403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GYN3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051150">
        <property type="entry name" value="SWT21/TCAB1_mRNA_Telomere"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13211">
        <property type="entry name" value="TELOMERASE CAJAL BODY PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13211:SF0">
        <property type="entry name" value="TELOMERASE CAJAL BODY PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7756403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="570" mass="64049" checksum="4793AF72F72BDB44" modified="2024-05-29" version="1">MPVGQEKGEVDLPSVSTVSKICKTSEVPSQSLTDRVIKYTFQRKRKRGALIDGNGSSERSHENQAAHVGAVMVNLIGESTPEKINIQWEQVARQVLVLSGEESDNNNEKDKKEHLLGFAKNRSIILHIIRVHLSKLYKIIEFIAAVKMGEQLQQENADQEVEEIMEEQTTAESQSECYSWPPAMRFDVPPYKTYHFYRHFRPSSNPNNFLKGVKWSPDGSCFLTSSDDNTLSVFTLPDYENCAEENVCSSAAIEDSFPANLAITEGEAIYDYCWYPYMSSSDPVSCVFATTTRDHPIHLWDTSTGQLRCTYRAYDNMDEITAAFSIGFNPTGTKIFAGYNKSIRIFDIHRPGRDFEQRSTIQGNKEGQSGIISSIAFSPSHTGMLATGSYSQTTAIYREDNMEQLYVLHGQEGGVTHVQFSKDGNYLYTGGRKDPYILCWDIRKTVEIVYKLYRSTETTNQRIQFDIEPYGRHLGTGGQDGLVHIYDLQTGQWASSFQAASDTVSGFSFHPFLPMAATASGHRRFSTLDDSDGDLSLTLRDDENCASVWSFSVYSADDYTANAEHVDSEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BJT3</accession>
    <name>A0AAD5BJT3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PCI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_017283</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the proteasome subunit S3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012222">
        <property type="protein sequence ID" value="KAI7724440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BJT3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042176">
        <property type="term" value="P:regulation of protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050756">
        <property type="entry name" value="CSN3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013586">
        <property type="entry name" value="PSMD3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057985">
        <property type="entry name" value="TPR_PSMD3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10758:SF2">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10758">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 3/COP9 SIGNALOSOME COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08375">
        <property type="entry name" value="Rpn3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25573">
        <property type="entry name" value="TPR_PSMD3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0647">Proteasome</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="4">
        <location>
            <begin position="96"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="296"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="13"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="300"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7724440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="332" mass="37184" checksum="62C83142519D6054" modified="2024-05-29" version="1">MTQDVEMKEQQVPAAAAAPSNSTSSTTPVLQHLKEIAALIETGAYAREVRRILRAVRLTIALRRKLKASVVSSFLGFALAPGSEAHSRLTSYLPKDDQSDMEVDTAASGTQATVKHSSELEIYCYLLVLIFLIDQKKYSEAKALSFYSKGVYFSQPQFLVFQAVRIGDLELFKTIAEKFSTTFSSDRTNNLIVRLRHNVIRTGLRNISISYSRISLVDVAKKLRLDSPNPVADAESIVSKAIRDGAIDATLDHANGWMVSKETGDIYSTNEPQQAFNSRIAFCLNMHNEAVRALRFPPNSHKEKESAEKRRERQQQEQELAKHIAEEDDDDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BKT9</accession>
    <name>A0AAD5BKT9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">Carbonic anhydrase</fullName>
            <ecNumber evidence="2 6">4.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_006973</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Reversible hydration of carbon dioxide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 6">
            <text>hydrogencarbonate + H(+) = CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:10748"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="EC" id="4.2.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the alpha-carbonic anhydrase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.1" evidence="2 6"/>
    <dbReference type="EMBL" id="JAMZMK010012149">
        <property type="protein sequence ID" value="KAI7724859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BKT9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004089">
        <property type="term" value="F:carbonate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03124">
        <property type="entry name" value="alpha_CA_prokaryotic_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.200.10">
        <property type="entry name" value="Alpha carbonic anhydrase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041891">
        <property type="entry name" value="Alpha_CA_prokaryot-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001148">
        <property type="entry name" value="CA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036398">
        <property type="entry name" value="CA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023561">
        <property type="entry name" value="Carbonic_anhydrase_a-class"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018338">
        <property type="entry name" value="Carbonic_anhydrase_a-class_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952:SF201">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00194">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM01057">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51069">
        <property type="entry name" value="Carbonic anhydrase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00162">
        <property type="entry name" value="ALPHA_CA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51144">
        <property type="entry name" value="ALPHA_CA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alpha-carbonic anhydrase" evidence="7">
        <location>
            <begin position="1"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7724859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="304" mass="35432" checksum="4065226746B276C5" modified="2024-05-29" version="1" fragment="single">MGPNRWGALRNDWSLCSNGTMQSPIDMSSRRVELVDHPKNLYRNYKPCNATMLNRGHDIMVKWMGDAGSIIINDTEYALKQAHWHSPSEHTINGIRFDMELHMVHLSIDNKIAVIAVLYRIGRPSHFLSKIQMSSRMYYRYMGSLTVPPCTEQVVWTLSRRNIMQDQYNHTIYETFFFMPRLERGNRNVITIHNPLKFNATQLLVSKLWQIQADTFDMELHMVHLSIDNKIAVIAVLYRIGRPSHFLSKLALNISAMIDRKGMREHSGIVNPREIQMSSRMYYRFMGSLTVPPCTEEVVWTLSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BKU3</accession>
    <name>A0AAD5BKU3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_013898</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012039">
        <property type="protein sequence ID" value="KAI7725152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BKU3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008239">
        <property type="term" value="F:dipeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04692">
        <property type="entry name" value="NUDIX_Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039461">
        <property type="entry name" value="Peptidase_M49"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23422">
        <property type="entry name" value="DIPEPTIDYL PEPTIDASE III-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23422:SF9">
        <property type="entry name" value="ZN-DEPENDENT HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="3">
        <location>
            <begin position="23"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="514"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7725152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="514" mass="58096" checksum="12B5DA3619688809" modified="2024-05-29" version="1" fragment="single">QFQVGTTNTNCRHDKSAVHRDGDYHRAVHIWIFAESTQQLLLQKRTDWKDSWPGLWDISSAGHVSAGDTSLITARRELQEELGITLPNDAFELLYVYLQESVTNDGKFIDNELDDVYLVTTLDPIPLEAFTLQESEVSAVKYISIEDYKHLLAKGDPHHLTGITEEDKEVLVLLIQAARIMDDIFYQQVWNSNPSLREWLNGRAQSSELDMLKWKSCLDENEAFLTTADSAVKLLSEATKPVANWKGLEYKAAFPILKPSGANFYPPDMDKMEFESWIESLSENEKQDATGFFNATFEAFIGIRDDKATAQVKLFGDHLQLLEQNLPMDDIYKSPDVIAAPIRVIQLVYNSGDVKGPQTVAFNLPNDERIVKDRGTSMVMLKNVSEAKSYKNFTRLLKKLKLTLLAFGPLIFLLLSKGQALQFNWLFEKGAFVLHSDQTFSVDFDKIEGAVESLSREILTIQAKGDKDAAQMLLEKYGKMTQPLNVALENLAKVQVPVDITPDFPIVTDLLHKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BKY7</accession>
    <name>A0AAD5BKY7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NLE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_014638</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011991">
        <property type="protein sequence ID" value="KAI7725242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BKY7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012972">
        <property type="entry name" value="NLE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848:SF0">
        <property type="entry name" value="NOTCHLESS PROTEIN HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08154">
        <property type="entry name" value="NLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50007">
        <property type="entry name" value="PIPLC_X_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="NLE" evidence="6">
        <location>
            <begin position="13"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="106"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="148"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="270"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="312"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="344"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7725242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="368" mass="40795" checksum="972D18B41BDB3EAD" modified="2024-05-29" version="1">MEVEGSREVNNNVMCQLSDPEGTPLGASMYLPQTVGPKELQQMVNKLLNNEEKLPYAFYISDHELVLQLGDYLQKNKVSVEKVVTIVYQPQAVFRIRPVNRCSSTIAGHEEAVLSVAFSPDGQQLASGSGDTTVRLWDLHTQTPLFTCTGHKNWVLCIAWSPDGKHLVSGSKSGELQCWNPLTGKPSGNPLIGHGHWVNSLALSTEYVLRTGAFDHTRKQYSSPEEMKTAALERYNKMKGNAPERLVSGSDDFTMFLWEPSVSKHPKTRMTGHQQLVNHVYFSPDGQWIASASFDKSVKLWNGITGKFVAAFRGHVGPVYQISWSADSRLLLSGSKDSTLKVFAVDWSPDGEKVASGGRDRVLKLWMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BL85</accession>
    <name>A0AAD5BL85_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_025452</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011936">
        <property type="protein sequence ID" value="KAI7725447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BL85"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11064">
        <property type="entry name" value="CYP86A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24296">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0349">Heme</keyword>
    <keyword evidence="5 6 7" id="KW-0408">Iron</keyword>
    <keyword evidence="3 6 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="6">
        <location>
            <position position="350"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7725447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="402" mass="46080" checksum="AB90BF41BAD58084" modified="2024-05-29" version="1" fragment="single">TGTRFIALLEDFLGRGIFNSDGETWRAQRKTASYEFNTRSLRNFVMETAAVELHMRFVPVLERAAALNKVVDLQDLLERYSFDNICKVAFNFDPGCLAGDGTSGSEFMKAFEEAATLSSARFMYILPGLYKIKKLLNFGSESKLRKSIATVHKFADDIIQSRITESTKEPNDDLLSRFMSISEYSPELLRDIVISFILAGRDTTSSALTWFFWLLSSHPEVKQKILNELNTLRLSSNKSDHNSYVLEDLRQMHYLHAAISEAMRLYPPVPVDTKACLKPDILPDGTFIGAGWFVTYHTYAMGRMESVWGTDCNEFRPERWLDEENDDGMMVYRQENPFKYPVFHGGPRVCLGKEMAYTQMKLLAATIMEAFEVEVEEKKVPEHVLSLTMRMKDGLKVRVRKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BLI4</accession>
    <name>A0AAD5BLI4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SWIM-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_014794</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011894">
        <property type="protein sequence ID" value="KAI7725612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BLI4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018289">
        <property type="entry name" value="MULE_transposase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000270">
        <property type="entry name" value="PB1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004332">
        <property type="entry name" value="Transposase_MuDR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006564">
        <property type="entry name" value="Znf_PMZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31973">
        <property type="entry name" value="POLYPROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31973:SF149">
        <property type="entry name" value="SWIM-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03108">
        <property type="entry name" value="DBD_Tnp_Mut"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10551">
        <property type="entry name" value="MULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00564">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04434">
        <property type="entry name" value="SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00666">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00575">
        <property type="entry name" value="ZnF_PMZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50966">
        <property type="entry name" value="ZF_SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SWIM-type" evidence="6">
        <location>
            <begin position="795"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="113"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="901"/>
            <end position="920"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="173"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="196"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="217"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="227"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="285"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="911"/>
            <end position="920"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7725612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="1036" mass="116039" checksum="0CEBD327ABCBEECF" modified="2024-05-29" version="1">MAKGKLILICQSGGEFVTNDDGTMSYSGGEANAANVTSETPFSDLKLNLAETCDLNQDTVTVKYFLPGNKRNLITVKNDKDVKRMIDFHGDAITAEVFVMGTPGFNRSALEMQTKRQNPTENDETVNNDNLSAKKDEKIKQGKAGPKKEKKVKKEDKVAKSPIASPTRRTRRSVAAAAAAANAEASSNKKRKQKGKQKEKEKEKEKENSDSDDSSKSEGSTGDTSSNAETEQVNVTVDSDCSPDFVPSRYPKRKKADASPADSVKKQRRTPSWKVGANGRPTIVSESAGSKSRGSSGNKTSQRKSGRLAAKGKNSQEKKSGKKRGRPPAITSDKEQEDVVGPTALDMCDDDVAPETLVSLWKSAITGVGQQFTNVHEFQETLRRYAIANDFEFKLKKNDKNRVVGECAGEGCSWKINAVWVPSTESFKIKTMNNVHKCDKESNNWLLNTIKEKLQESPHLKPKEIANGLLQDFKVDSNRTEVFDGKAISREQLHGSDKDAYNKLPWFSEKIMETNPGSFSKLVIGENKRFKALFVSFYSSLCGFLNGCRPLLFLEATSTWSKYGEVLITANAIDGNDDFFPVAFAIVDVEDDNNWRWFLGQLKATIFNSQQPITFVFDREKNLKTSVLELFEGSNVGYSIYHLLESFKRNVKGPFLGDGKSFLPYYFLAAAHAVRLVGFKKSTEQIKQISSQAYDWVMQTEPEHWATSYFKGERYNHIIDDVSGSLSKLMEEYRELPILHKIDAIIRMMIDAVNDAKLDGSMWSTHLTPSKEKQLQEENLKSSGLKVLISSDTLFEVREDSTHVVNTSTWSCTCLGWKETGLPCRHALAVCALIGRSPYEFCSSYFTVDAYRLTYFEPINPVPIEREEAEKIEIEQVGGEKEQAFIVEDVHAETGEKIQAEEEKVEIETESETKTDKEECEKVEIEDEKGEKMEIEITWGDNEEGGKVEGEKVEIEKDEGEKGKIAADEEDGLRHVLVLPPIPVQTVDVMKEKMDWDEAEVETKRTVTCTKCKQPGHNKKSCNLYQLQQQDTSQQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BNA4</accession>
    <name>A0AAD5BNA4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_029677</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011975">
        <property type="protein sequence ID" value="KAI7725329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BNA4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000095">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046956">
        <property type="entry name" value="RLP23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063:SF106">
        <property type="entry name" value="LEUCINE-RICH REPEAT DOMAIN, L DOMAIN-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063">
        <property type="entry name" value="LRR RECEPTOR-LIKE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="210"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="240"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="576"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7725329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="576" mass="64310" checksum="97C059B69203E391" modified="2024-05-29" version="1" fragment="single">IHLQRLNLSNSSIRDTIPEWFENIMSHIGYLDLSNNQISGKLPRFDSDSSVMDVSNNTITGVIPSSLGSLTYLQSLHLHNNRLEGNLPLSLQNLTSLVTLDTGNNFLMGTIPFWIGESLLNLRILNLQSNKFTSKIPQQLCQLNALQHLNLANNNLTGTIPHCFSNLSGMIINPGNNDLCGPPVSKSCKGNSPTYKHVGEEEGQDDDEGLWFYFGMGPGFVVGLMGLLGSLHFIRRWRVAYFGMLENVYGWLIVSVLVPFINGCVKEWMVNLHHVLNLGTEADNAQLFGVSGEVDYHILSLSLRNLLQAKNPNQERSFESEHYCRLLHLLFKVLILRMNSNKFEGSLATFPSNVWILDLSDNLLSGHVPQTDGTMNPRLQVVNLSKNRFNGSIPVHLCKLPTIYVFDLSQNKFSGRLPSCLGNLFGLQVMDVSNNTITGVIPSSLGSLTFLKSLHLHNNRFEGNLPLSLRNLTCLVTLDVGNNFLIGRIPFWIGESLLDLRILNLQLNKFTGKIPLQLCQLNALQHLNLAQNNIIGMLPHCLSNLSGMIINSNDSVYFQDSDYEENILAYMKGIQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BPJ4</accession>
    <name>A0AAD5BPJ4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">polynucleotide adenylyltransferase</fullName>
            <ecNumber evidence="4">2.7.7.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">M8C21_020170</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">AA19_3_7</strain>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="13">Binds 2 magnesium ions. Also active with manganese.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the poly(A) polymerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.19" evidence="4"/>
    <dbReference type="EMBL" id="JAMZMK010011434">
        <property type="protein sequence ID" value="KAI7727145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BPJ4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990817">
        <property type="term" value="F:poly(A) RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031123">
        <property type="term" value="P:RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05402">
        <property type="entry name" value="NT_PAP_TUTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.460.10:FF:000002">
        <property type="entry name" value="Poly(A) polymerase alpha, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.590">
        <property type="entry name" value="Poly(A) polymerase predicted RNA binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011068">
        <property type="entry name" value="NuclTrfase_I-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007012">
        <property type="entry name" value="PolA_pol_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048840">
        <property type="entry name" value="PolA_pol_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007010">
        <property type="entry name" value="PolA_pol_RNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014492">
        <property type="entry name" value="PolyA_polymerase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10682">
        <property type="entry name" value="POLY A POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10682:SF22">
        <property type="entry name" value="POLYNUCLEOTIDE ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04928">
        <property type="entry name" value="PAP_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20750">
        <property type="entry name" value="PAP_NTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04926">
        <property type="entry name" value="PAP_RNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018425">
        <property type="entry name" value="PolyA_polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55003">
        <property type="entry name" value="PAP/Archaeal CCA-adding enzyme, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81631">
        <property type="entry name" value="PAP/OAS1 substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Poly(A) polymerase nucleotidyltransferase" evidence="17">
        <location>
            <begin position="14"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="Poly(A) polymerase central" evidence="16">
        <location>
            <begin position="249"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="Poly(A) polymerase RNA-binding" evidence="15">
        <location>
            <begin position="339"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="518"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="521"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="531"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="564"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="618"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="632"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="93"/>
            <end position="95"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="106"/>
            <end position="108"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="222"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="240"/>
            <end position="241"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="18">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR018425-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR018425-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF04926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF04928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF20750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAI7727145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="665" mass="75614" checksum="A9EA917DAD67699C" modified="2024-05-29" version="1" fragment="single">MVGSEALPSPRQHGVTKPLSLAGPSDADLLRTKKLNKFLLDAGLYGSQEEAAKREKVLGRIKQIVVDWVKELTRLRGYSDQMVEDANAVIFTFGSYRLGVHGPGADIDTLCVGPSYVNRDEDFFFGLHEMLTKMEEVTELQPVPSAHVPVMKFKFEGISIDLLYASISRLVVPDDLDISDVSVLYNVDEPTVRSLNGCRVADQILKHVPNVEHFRTTLRCLKFWAKRRGVYSNVTGFLGGVNWALLVARWRWPNPVLLCAIQENEELGCTVWDPRKNPKDKTHHMPIITPAYPCMNSSYNVSISTLRVMTEQFQFGNRICEEIELNKAQWPMLFEPYMFFESYKNYLQVDIVAADPDDLRAWKGWVESRLRQLTLMVMSNPSNRYLLMVMVMVPLVTISTYRWQLARWEVGWVETVELKLIERDTQGKLQCHPYPHDYIDPSKQCSHNAFFMGLQRKQGEIVQENQQFDIRGTVEEFRHSVNMYMFWKPGMEIYVSHVRRKQIPLYVFPDGYKRARIPRSMNHQPVSGSDSAEKPSLKRKGEGHVERDSPQRRKSVSPIPITSPFMSPQSRGSDSSDMYSHSLANGPEPESNFKSEEVGLIGCSPGSSIVTNEEVGTEKTSLGSNTQDSSEEDSHSAVDKLSGDQIQVRRNINCLRGSKSISHLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BPV4</accession>
    <name>A0AAD5BPV4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_003498</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011396">
        <property type="protein sequence ID" value="KAI7727352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BPV4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043240">
        <property type="term" value="C:Fanconi anaemia nuclear complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006513">
        <property type="term" value="P:protein monoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23831">
        <property type="entry name" value="DRWD-N_FANCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.20">
        <property type="entry name" value="RWD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026848">
        <property type="entry name" value="Fancl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043898">
        <property type="entry name" value="FANCL_d2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044037">
        <property type="entry name" value="FANCL_d3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043003">
        <property type="entry name" value="FANCL_d3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13206:SF0">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE FANCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13206">
        <property type="entry name" value="UBIQUITIN LIGASE PROTEIN PHF9 FANCONI ANEMIA GROUP L PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18890">
        <property type="entry name" value="FANCL_d2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18891">
        <property type="entry name" value="FANCL_d3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FANCL UBC-like" evidence="1">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="FANCL UBC-like" evidence="2">
        <location>
            <begin position="75"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7727352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="181" mass="20993" checksum="0C071F3F25882B2C" modified="2024-05-29" version="1" fragment="single">IEEIGWEHLLKLAEDLSDKKERVHVLEILLDKSYPNCPPSVSADVPYLFDLEWSRNSRLNDVVKQFRQHLEKLQQFWSAVEDIDQSLWVYDSAQLHRATSFRHINIGNDCSIMLHIRANEPRSLPENRENVNLIHPAQYNQASPTNAAQPKSNIIKHAVFCSSCFYDLVHPFSHVCFIVCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BRR6</accession>
    <name>A0AAD5BRR6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cupin type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_030013</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 11S seed storage protein (globulins) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011211">
        <property type="protein sequence ID" value="KAI7728461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BRR6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045735">
        <property type="term" value="F:nutrient reservoir activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02243">
        <property type="entry name" value="cupin_11S_legumin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02242">
        <property type="entry name" value="cupin_11S_legumin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006044">
        <property type="entry name" value="11S_seedstore_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006045">
        <property type="entry name" value="Cupin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050253">
        <property type="entry name" value="Seed_Storage-Functional"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31189">
        <property type="entry name" value="OS03G0336100 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31189:SF45">
        <property type="entry name" value="OS09G0552500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00190">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00439">
        <property type="entry name" value="11SGLOBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00835">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0708">Seed storage protein</keyword>
    <keyword evidence="2" id="KW-0758">Storage protein</keyword>
    <feature type="domain" description="Cupin type-1" evidence="5">
        <location>
            <begin position="12"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="Cupin type-1" evidence="5">
        <location>
            <begin position="198"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7728461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="358" mass="37999" checksum="C66F7DEFB30A86EC" modified="2024-05-29" version="1">METKMVVQKADLTAFEGDSGGYYVWSNEKTPLLSDTKLAAGKLLLHPLGFALPHYADCNKIGFVLEGAITIGLVPPNSSEETVLLIKKGDVVPLIAGVVSWWFNGGDTDAVIVFLGDSSKALIPGQFTYFFVAGVLGLLAGFQPDFVGKTFGLDETESENVVNSQQGALLVKLDQGIKFPEPSKHTKNKLYTTIDDPSGAVAVKGGGFINYLTEKKLPMLSEIGLSAKFVKLEGNAMLAPSYVADGSVQICYVGKGSGWIKVVGSEGKPALDSIVEEGDLFIVPQFFGLAGIADDCGMEVFSVLTSSKPVFGQLAGNTSVWNALAPVVLQATLNVTSEFEQLFKSKNSESITIVPPST</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BRT0</accession>
    <name>A0AAD5BRT0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Receptor-like protein 12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_032974</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RLP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011273">
        <property type="protein sequence ID" value="KAI7728099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BRT0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000111">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046956">
        <property type="entry name" value="RLP23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48061">
        <property type="entry name" value="LEUCINE-RICH REPEAT RECEPTOR PROTEIN KINASE EMS1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48061:SF2">
        <property type="entry name" value="RECEPTOR LIKE PROTEIN 30-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 12" id="KW-0732">Signal</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042176914" description="Receptor-like protein 12" evidence="12">
        <location>
            <begin position="22"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="741"/>
            <end position="765"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7728099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="792" mass="89486" checksum="2E39378E914D0AA8" modified="2024-05-29" version="1">MSNSIMLFFLLSLFFISSTSSSSIKTIPKCSVQQTHALLLLNKTFPLSMINWDSRCEDWLGTIHPNTTLFYLPHLRRLNLAFNFFTNFQLPHDIGRVSNSLTHLNISCCGYTGQIPTDISHLHKLVSLDLSFNQNGFDYLKLEPHVFINLLQNSTVMEQLSLENVNISLLLPTNLNISSSSSLKLLNLRYTRLQGNLPHNIFNLQSLETLDFLTAHIPSEISLLLKLVSPDLSVNGNEIDTLLIQPHIFRILLTNSTILRDLRLDEVNIGFLLLTYLNISSALRSLGLSYTNLQGKLPHNIFNLEYLEELYLSGNSNLSGPFPKVNTTSTIPLRQLYLSKTSLSGEIRTSIGYLKSLEYLDLSYCGFNGSLPKPLGTLSLANNQLTGQIDVLDNGPGLQTFQRLTNLNDLDLSYNNFRGDWELDALLSSLTILESLVLSYSGISVTTSNGIHYVNPHLWILSLASCSLKVFPVSIRAMKNLQHLDLSNNDIQGHIPDWIGEIGRNQLFALNIANNSGVYFERKSVRRKSAHFFEQMSKIDSSCPQMKQFSWCHCNFFQGQLPTNYFQNFNVMKNVLRDNTKPNYVEMGSICERSRSIFSKLLVNYTIVDLSDNKFHGEIPNITGYLKCLKVLNLSHNSLTGRIPHALGLISEIESLDLSWNQLIGEIPQSLADLTFLEFLNLSQNHLMGRIPQGRQFNTFDGYSYSGNPKLHGLLLPSKCSEHLPKPQPKGDEDGQQENGFTWKVVVMGYGCGALLGIVMGYLMLSTEKPKWFNAIFDDVEHFILKRQNKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BTA0</accession>
    <name>A0AAD5BTA0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">glutamate synthase (ferredoxin)</fullName>
            <ecNumber evidence="17">1.4.7.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">M8C21_016138</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">AA19_3_7</strain>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="16">Amino-acid biosynthesis; L-glutamate biosynthesis via GLT pathway; L-glutamate from 2-oxoglutarate and L-glutamine (ferredoxin route): step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Energy metabolism; nitrogen metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Nitrogen metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the glutamate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.4.7.1" evidence="17"/>
    <dbReference type="EMBL" id="JAMZMK010011039">
        <property type="protein sequence ID" value="KAI7729212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BTA0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051538">
        <property type="term" value="F:3 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016041">
        <property type="term" value="F:glutamate synthase (ferredoxin) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016040">
        <property type="term" value="F:glutamate synthase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019676">
        <property type="term" value="P:ammonia assimilation cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006537">
        <property type="term" value="P:glutamate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050711">
        <property type="entry name" value="ET-N_metabolism_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11938">
        <property type="entry name" value="FAD NADPH DEHYDROGENASE/OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11938:SF133">
        <property type="entry name" value="GLUTAMATE SYNTHASE (NADH)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00310">
        <property type="entry name" value="GATase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0003">3Fe-4S</keyword>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0288">FMN</keyword>
    <keyword evidence="14" id="KW-0314">Glutamate biosynthesis</keyword>
    <keyword evidence="10" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="12" id="KW-0408">Iron</keyword>
    <keyword evidence="13" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="18">
        <location>
            <begin position="97"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="19">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00037928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00039085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAI7729212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="270" mass="29759" checksum="F112EF5EF821E0F0" modified="2024-05-29" version="1" fragment="single">MSVTFNNVFQTRPDPNLTTPFSNRPLTGFSRVRVGVRQSSLLGKKLVGSGSGSGLVRVRDWKRVDPKRGGVVVRSNLSLVPEKPLGLYDPRFDKDACGVGFVAELSGESNRKTVTDAIEMLVRMSHRGACGCETNTGDGAGILVGIPHDFYKEVAESLGHTVLGWRTVLTDNSSLGKSALQTEPVIEQVFLTPTSRSKADFEQQMYILRRVSMVAIRAALNLQHGGVKDFYICSLSSRTVVYKGQLKPNQLKEYYYADLGNERFTSYMAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BVV7</accession>
    <name>A0AAD5BVV7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_025362</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Cu(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29036"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tyrosinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010782">
        <property type="protein sequence ID" value="KAI7730417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004097">
        <property type="term" value="F:catechol oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1280.10">
        <property type="entry name" value="Di-copper center containing domain from catechol oxidase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008922">
        <property type="entry name" value="Di-copper_centre_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022740">
        <property type="entry name" value="Polyphenol_oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022739">
        <property type="entry name" value="Polyphenol_oxidase_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050316">
        <property type="entry name" value="Tyrosinase/Hemocyanin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002227">
        <property type="entry name" value="Tyrosinase_Cu-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11474:SF119">
        <property type="entry name" value="CATECHOL OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11474">
        <property type="entry name" value="TYROSINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12142">
        <property type="entry name" value="PPO1_DWL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12143">
        <property type="entry name" value="PPO1_KFDV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00264">
        <property type="entry name" value="Tyrosinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00092">
        <property type="entry name" value="TYROSINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48056">
        <property type="entry name" value="Di-copper centre-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0186">Copper</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0883">Thioether bond</keyword>
    <feature type="domain" description="Tyrosinase copper-binding" evidence="9">
        <location>
            <begin position="172"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="Polyphenol oxidase central" evidence="10">
        <location>
            <begin position="397"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="domain" description="Polyphenol oxidase C-terminal" evidence="11">
        <location>
            <begin position="460"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="50"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF12142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF12143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7730417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="509" mass="57244" checksum="C88AC67524F8B639" modified="2024-05-29" version="1">MMTSLCLSTFPTITTSQSPLLSKPSSNIIRFKVSCNASNSDNHLKTPDENPNIIHNNNNNNVDRRNLLLGLGGLYGATNLTNIGSALAYPITAPDDISHCEKASTNIKHPEDAVRGLACCPPESPAYPKPYVLPSFHKLRVRRPAHKLTPDYIKKYQNAIKKMKALPDEHPHSWKQQGKIHCAYCNGGYTQEMNGRDDLKIQVHGSSLFYPFHRWYLYFYERILGKLIGDPTFALPYWNWDNPMGMMLPAMFEAPGDSNDPKNNPLFDPYRDAGHRPPVIIDLQYSGVDSGAISVDQISDNLSTMYNQMVRARCTDAFFGTDPDPERESLYGYIESGAHTAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDPTSDDWLNASYVFYDENEELVRVYNKDCVNMRRMGYMYEKSSIPWVKSRPTPRRKAGKVGLKLDGVATFEIDEILEDIEAEDDEHVLVTLVPRAGTSDATVSKIKIELVPTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BYS8</accession>
    <name>A0AAD5BYS8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Isopropylmalate dehydrogenase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_027049</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010557">
        <property type="protein sequence ID" value="KAI7731063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BYS8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004449">
        <property type="term" value="F:isocitrate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006102">
        <property type="term" value="P:isocitrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.718.10">
        <property type="entry name" value="Isopropylmalate Dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835">
        <property type="entry name" value="DECARBOXYLATING DEHYDROGENASES-ISOCITRATE, ISOPROPYLMALATE, TARTRATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835:SF64">
        <property type="entry name" value="ISOCITRATE DEHYDROGENASE [NAD] REGULATORY SUBUNIT 1, MITOCHONDRIAL-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53659">
        <property type="entry name" value="Isocitrate/Isopropylmalate dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Isopropylmalate dehydrogenase-like" evidence="2">
        <location>
            <begin position="1"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7731063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="196" mass="21286" checksum="08D043638C543990" modified="2024-05-29" version="1" fragment="single">VITKFCSERIAKYAFEYAHLNNRKTVTAVHKANIMKLADGLFLESCREIAKSYPEIKYNEIIVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGVMPGGNVGVEYAIFEQGASAGNVGKPKLVEKKKANPVALLLSSAMMLRHLELPEFADRLESAVKRVIKEGKYRTKDLGGTSTTQEVTDAVISAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BZ85</accession>
    <name>A0AAD5BZ85_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_008951</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Co-chaperone that regulates diverse cellular pathways, such as programmed cell death and stress responses.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010350">
        <property type="protein sequence ID" value="KAI7731939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BZ85"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000774">
        <property type="term" value="F:adenyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17054">
        <property type="entry name" value="Ubl_AtBAG1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.120:FF:000011">
        <property type="entry name" value="BAG family molecular chaperone regulator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000298">
        <property type="entry name" value="BAG family molecular chaperone regulator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.120">
        <property type="entry name" value="BAG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039773">
        <property type="entry name" value="BAG_chaperone_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036533">
        <property type="entry name" value="BAG_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003103">
        <property type="entry name" value="BAG_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12329:SF11">
        <property type="entry name" value="BAG FAMILY MOLECULAR CHAPERONE REGULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12329">
        <property type="entry name" value="BCL2-ASSOCIATED ATHANOGENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02179">
        <property type="entry name" value="BAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00264">
        <property type="entry name" value="BAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63491">
        <property type="entry name" value="BAG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51035">
        <property type="entry name" value="BAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="4">
        <location>
            <begin position="45"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="BAG" evidence="5">
        <location>
            <begin position="140"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="225"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00058673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7731939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="271" mass="30836" checksum="794C1A4B2CD508C1" modified="2024-05-29" version="1">MMHMKTKSTEPPSVNGGDKEWEVRPGGMLVQRRDPTEEQNRLPPPTIRVRVKYGSIYHEINISSQATFGELKKMLSGPTGLHHEDQKLMYKDRERSSKTFLDVVGVKDRSKMVLVEDPISQEKRYIEMRKNAKMEKAAKSISEISLEVDRLAGQVSAFESVISKGGKVAEKTLLNLIELLMNQLLKLDGITAEGDVKLQRKMQVKRVQKYVETLDVLKIKNSITGTNENGDEKQVKEPPASQGQEERFSSNGHRRLNSNNHQGTRRQGRLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C0T6</accession>
    <name>A0AAD5C0T6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PB1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_029835</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010096">
        <property type="protein sequence ID" value="KAI7733042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C0T6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045012">
        <property type="entry name" value="NLP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055081">
        <property type="entry name" value="NLP1-9_GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053793">
        <property type="entry name" value="PB1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000270">
        <property type="entry name" value="PB1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32002:SF35">
        <property type="entry name" value="PROTEIN NLP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32002">
        <property type="entry name" value="PROTEIN NLP8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22922">
        <property type="entry name" value="GAF_NLP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00564">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00666">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51745">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PB1" evidence="2">
        <location>
            <begin position="471"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="341"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="341"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="363"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7733042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="560" mass="62135" checksum="AE6D33E3B5BB0DDA" modified="2024-05-29" version="1" fragment="single">KDSEHKAFLVDKDHEEEDLSPPARVFRRGLPEWTSDLNNYKGKDFPQQDCAIRCNLHGYLALPVFDSTTGSCVGVLEILTSIKYMCYAFEVQQVHKALEAENLTSPQVFDWPATKVPTGSRQNKLDKIFTILKGVCDSHSLPLAQAWTVSSPGSFVAHNEVIEKTCSSFDTRCIGKVCMSTAALPFHVGDLDMWHFRKACSEQHLDKSHGLVGKAFLARGSCYCHDVTELSEEEYPLVHYARMSGLTSCFTIFLHSVEGDDKDDYVLEFFLQLGNKDSRHVLDLVQTLKHLVEADSGFELGEISPIEVTEPLRDVSHLSLSTKPHTNQISSTTMANALTVETDSSDSGSLSPNGAKKDPANVAGQQSPNQIHPNTQEGSIIDNVSMQKTKQGRKRKIDSLSMDAVKQNVGKPLDQTAQILVSRSTLKRFCREHGISSWPVPKHIKRSDFGSQKHLQQSSTQSVGHVSDTGLLMVKATFKDDMIKFPFPVSSGLVELEKEVAERVDVKGQRFSLKYEDEDKDLVLISCDDDLKSLASYPTIKLLVHVADDGRLANEASHGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C184</accession>
    <name>A0AAD5C184_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Sec1-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_009719</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the STXBP/unc-18/SEC1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010058">
        <property type="protein sequence ID" value="KAI7733252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C184"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001619">
        <property type="entry name" value="Sec1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027482">
        <property type="entry name" value="Sec1-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036045">
        <property type="entry name" value="Sec1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11679">
        <property type="entry name" value="VESICLE PROTEIN SORTING-ASSOCIATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00995">
        <property type="entry name" value="Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56815">
        <property type="entry name" value="Sec1/munc18-like (SM) proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="46"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="135"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="46"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="144"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7733252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="154" mass="17258" checksum="663AB45C652B5227" modified="2024-05-29" version="1" fragment="single">ELIEKLSKNELPVNEYPCMNDPSPTFHGASHSVSARVLDTPCAHSKRSRRSSWARPRHSEDGSSSESILRHAITEYQKMGKRIFVFIVGGATRSELRVCHKLTTNLRREIILGSSSLDNPAQFVEKLKSLTPIVDENENKNENETEVAETEVVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C186</accession>
    <name>A0AAD5C186_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_013298</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010077">
        <property type="protein sequence ID" value="KAI7733145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C186"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055087">
        <property type="term" value="C:Ski complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039226">
        <property type="entry name" value="Ski3/TTC37"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15704">
        <property type="entry name" value="SUPERKILLER 3 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15704:SF7">
        <property type="entry name" value="SUPERKILLER COMPLEX PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13414">
        <property type="entry name" value="TPR_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="167"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7733145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="225" mass="25322" checksum="0D340C9D8ACFFA91" modified="2024-05-29" version="1">MGDEKDSQDDSVFTQLSEAVATNPDDPSARFNLGLFLWDRTGEESKQKAAEHFVIAAKLNPQDANAFRYLGHYYSKVSIDTQRALKCYQRALALSPQDSESGESLCDLLDKEGKESLEVAVCREASGKSPRAFWAFRRLGFLQVHQKKWPEAVQSLQQAIRGYPTSADLWEALGLAYQRLSMFTAAIKSYGRAIELEESRVFSLVESGYVFLMLGSHRKVILTHM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C236</accession>
    <name>A0AAD5C236_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">SCP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_012495</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Probably involved in the defense reaction of plants against pathogens.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CRISP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009931">
        <property type="protein sequence ID" value="KAI7733672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C236"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098542">
        <property type="term" value="P:defense response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05381">
        <property type="entry name" value="CAP_PR-1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.33.10:FF:000004">
        <property type="entry name" value="CAP, cysteine-rich secretory protein, antigen 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.33.10:FF:000006">
        <property type="entry name" value="Putative pathogenesis-related protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018244">
        <property type="entry name" value="Allrgn_V5/Tpx1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001283">
        <property type="entry name" value="CRISP-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002413">
        <property type="entry name" value="V5_allergen-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10334">
        <property type="entry name" value="CYSTEINE-RICH SECRETORY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00838">
        <property type="entry name" value="V5ALLERGEN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00837">
        <property type="entry name" value="V5TPXLIKE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00198">
        <property type="entry name" value="SCP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="PR-1-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01009">
        <property type="entry name" value="CRISP_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01010">
        <property type="entry name" value="CRISP_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0568">Pathogenesis-related protein</keyword>
    <keyword evidence="4" id="KW-0611">Plant defense</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041906807" description="SCP domain-containing protein" evidence="7">
        <location>
            <begin position="25"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="SCP" evidence="8">
        <location>
            <begin position="34"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="SCP" evidence="8">
        <location>
            <begin position="170"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7733672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="292" mass="33746" checksum="C24E74891B5CF32D" modified="2024-05-29" version="1">MWFPKATSMILVFFLCILLSGTNAIPHPKKHSHHVIKDFMSLQNRARAAIHMPPLVWDRRLARYATMYARQRRQDCLLKHSNGPYGENIFWGSGTRWRASQAAATWVSEGRWYNHQSNSCNQGRECGHYTQIVWKTSRRIGCARVTCFGGRGVFMICNYDPPGNYIAKWEFPLGWDFQLQRYAQWWAGQRKGDCQLMHSFPEDDFKLGENIYWGSGSSWTPVDAVNTWAGEEKYYRYGTNMCASGQQCGHYTQIVWKTTRRVGCARVVCDTGDVFMTCNYDPVGNVIGERPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C3W9</accession>
    <name>A0AAD5C3W9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Pectinesterase inhibitor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_026159</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009948">
        <property type="protein sequence ID" value="KAI7733536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C3W9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004857">
        <property type="term" value="F:enzyme inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15798">
        <property type="entry name" value="PMEI-like_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.40">
        <property type="entry name" value="Invertase/pectin methylesterase inhibitor family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035513">
        <property type="entry name" value="Invertase/methylesterase_inhib"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006501">
        <property type="entry name" value="Pectinesterase_inhib_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051955">
        <property type="entry name" value="PME_Inhibitor"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01614">
        <property type="entry name" value="PME_inhib"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31080:SF295">
        <property type="entry name" value="PECTINESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31080">
        <property type="entry name" value="PECTINESTERASE INHIBITOR-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04043">
        <property type="entry name" value="PMEI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00856">
        <property type="entry name" value="PMEI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101148">
        <property type="entry name" value="Plant invertase/pectin methylesterase inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Pectinesterase inhibitor" evidence="2">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7733536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="103" mass="11337" checksum="B1A0DAC6E5E44B56" modified="2024-05-29" version="1">MSKVHGMTPREVAAMKNCIELLSDSVYMLQRSLEEMSQPGSKDSKLVMSDIQTWVSSALTDEDTCSEGFKDDPKMKRDVRGKTLNVACLTSNALINSYASLNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C606</accession>
    <name>A0AAD5C606_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Bifunctional dihydrofolate reductase-thymidylate synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">M8C21_019403</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">AA19_3_7</strain>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Bifunctional enzyme. Involved in de novo dTMP biosynthesis. Key enzyme in folate metabolism. Can play two different roles depending on the source of dihydrofolate: de novo synthesis of tetrahydrofolate or recycling of the dihydrofolate released as one of the end products of the TS catalyzed reaction. Catalyzes an essential reaction for de novo glycine and purine synthesis, DNA precursor synthesis, and for the conversion of dUMP to dTMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>(6S)-5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15009"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57451"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.5.1.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>dUMP + (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate = 7,8-dihydrofolate + dTMP</text>
            <dbReference type="Rhea" id="RHEA:12104"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:57451"/>
            <dbReference type="ChEBI" id="CHEBI:63528"/>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <dbReference type="EC" id="2.1.1.45"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 14">Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">In the C-terminal section; belongs to the thymidylate synthase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">In the N-terminal section; belongs to the dihydrofolate reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009673">
        <property type="protein sequence ID" value="KAI7734661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C606"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004146">
        <property type="term" value="F:dihydrofolate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004799">
        <property type="term" value="F:thymidylate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006231">
        <property type="term" value="P:dTMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00209">
        <property type="entry name" value="DHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00351">
        <property type="entry name" value="TS_Pyrimidine_HMase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.430.10:FF:000003">
        <property type="entry name" value="Bifunctional dihydrofolate reductase-thymidylate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.572.10:FF:000013">
        <property type="entry name" value="Thymidylate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.430.10">
        <property type="entry name" value="Dihydrofolate Reductase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.572.10">
        <property type="entry name" value="Thymidylate synthase/dCMP hydroxymethylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00008">
        <property type="entry name" value="Thymidy_synth_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024072">
        <property type="entry name" value="DHFR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012262">
        <property type="entry name" value="DHFR-TS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017925">
        <property type="entry name" value="DHFR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001796">
        <property type="entry name" value="DHFR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045097">
        <property type="entry name" value="Thymidate_synth/dCMP_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023451">
        <property type="entry name" value="Thymidate_synth/dCMP_Mease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036926">
        <property type="entry name" value="Thymidate_synth/dCMP_Mease_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000398">
        <property type="entry name" value="Thymidylate_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020940">
        <property type="entry name" value="Thymidylate_synthase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03284">
        <property type="entry name" value="thym_sym"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11548:SF2">
        <property type="entry name" value="THYMIDYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11548">
        <property type="entry name" value="THYMIDYLATE SYNTHASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00186">
        <property type="entry name" value="DHFR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00303">
        <property type="entry name" value="Thymidylat_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000389">
        <property type="entry name" value="DHFR-TS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00108">
        <property type="entry name" value="THYMDSNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53597">
        <property type="entry name" value="Dihydrofolate reductase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55831">
        <property type="entry name" value="Thymidylate synthase/dCMP hydroxymethylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00075">
        <property type="entry name" value="DHFR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51330">
        <property type="entry name" value="DHFR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00091">
        <property type="entry name" value="THYMIDYLATE_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 14" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="8" id="KW-0521">NADP</keyword>
    <keyword evidence="7 14" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="4 14" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="9 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DHFR" evidence="17">
        <location>
            <begin position="20"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="active site" evidence="15 16">
        <location>
            <position position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR000389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000389-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAI7734661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="536" mass="60082" checksum="C369BEC66DD8351E" modified="2024-05-29" version="1">MAGETVLGSCNGTVIIPQRTYQVVVAATRAMGIGKDGKLPWKLPSDLKFFKDVTMTTSDPAKKNAVIMGRKTWESIPLEHRPLPGRLSVVLTRSGSFDIATAENVLMCGSMISALELLASSPYCLSIERVFVIGGGEILRETLNGPGCEAIHITEIETNIDCDTFIPPIDASQFQPWYSSFPLVENGIRHCFTTYVRVRNPAVEDSVGTNGLLSNSSPDSTKFDSRMYSFLPKKIFENHQEYLYLRLVEDIISNGTLKGDRTGTGTLSKFGCQMRFNLRKSFPLLTTKACTFLKCFGVELWKNFCGSSVVQQVPRFYKIRAFIYGTVMHLEITWTGNSTPLLFIGLVDREEGDLGPVYGFQWRHFGARYTDMHADYTGQGFDQLLDVIEKIKNNPDDRRIILSAWNPSDLKQMALPPCHMFAQFYVNQGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCELVPGDFVHVLGDAHVYSTHVRPLQEQLQKQPKPFPTLKINPEKKNIDGFVADDFELVGYDPHQKIQMKMAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C6D4</accession>
    <name>A0AAD5C6D4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ion transport domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_010883</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009446">
        <property type="protein sequence ID" value="KAI7735719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C6D4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005216">
        <property type="term" value="F:monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45651">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-GATED ION CHANNEL 15-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45651:SF48">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-GATED ION CHANNEL 8 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0407">Ion channel</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="88"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="122"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="177"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="249"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="7">
        <location>
            <begin position="84"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7735719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="372" mass="42557" checksum="F5E98B3BEC436815" modified="2024-05-29" version="1" fragment="single">MMSSTGVMKRCRFNIEGATTNPGPSRPPRPTASKSLKLGMRRGSEGLINLGQTLKTGVTRAVFSEDLKVSEKKIIDPQDKNLLIWNKFLVLSCILAVYVDPLFFYIPVYHSNERCLKIHKSLAHYITTVRTIVDLFYLIRIGLQFRTAYIAPSSRVFGRGELVIDPAQIARRYMQRYFVVDLLSVLPLPQIVVWRFLHESHGSGVLATKNVLFWIVIVQYIPRSIRILPLFSELKKTVGVITETAWAGAAYYLVWFVLAGHIFGAFWYLLAVERKSSCWEQACIKAKKCEIDYLYCGYEETDSYKAWMNVSKATLDERCVENEAYGEFDYGIYLAAVQSKVDSSEKFISKYFYCLWWGLRNLSTLGQGLETS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C6K9</accession>
    <name>A0AAD5C6K9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_004828</name>
        <name evidence="3" type="ORF">M8C21_017816</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009476">
        <property type="protein sequence ID" value="KAI7735458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAMZMK010007680">
        <property type="protein sequence ID" value="KAI7743783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C6K9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35135">
        <property type="entry name" value="OS05G0517800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35135:SF3">
        <property type="entry name" value="OS05G0517800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042441275" evidence="2">
        <location>
            <begin position="16"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7735458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7743783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="71" mass="8005" checksum="D3945ABE65C1E86D" modified="2024-05-29" version="1">MALTNFILTVAGVSAVILLMRSDVKQSASIFKRNVRQIRHWLEEESKSAAKEVEKAAKPKELPKKDIPKDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C6U8</accession>
    <name>A0AAD5C6U8_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_002387</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009316">
        <property type="protein sequence ID" value="KAI7736307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C6U8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7736307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="93" mass="10009" checksum="DBF6B9773526489E" modified="2024-05-29" version="1">MVALPMETTTGTCFLQHYIDIADPDLKINHGDKASSGIIEKIVAVIQDPTELHVVTELYSWGSGVSYQSGTGNARIQKLPCKVDSLHGSLLPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C796</accession>
    <name>A0AAD5C796_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Disease resistance R13L4/SHOC-2-like LRR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_000486</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009257">
        <property type="protein sequence ID" value="KAI7736502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C796"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000041">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000400">
        <property type="entry name" value="Nuclear pore complex protein NUP107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051824">
        <property type="entry name" value="LRR_Rcpt-Like_S/T_Kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48006">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN DDB_G0281931-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48006:SF66">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042173119" description="Disease resistance R13L4/SHOC-2-like LRR domain-containing protein" evidence="7">
        <location>
            <begin position="31"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="8">
        <location>
            <begin position="144"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7736502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="445" mass="49033" checksum="9BA650BCE036FFC3" modified="2024-05-29" version="1">MMFKPSSTLFFIIMLILSMALFLQITGIQAQVGHLPPNEADALQEIAKQLGKEDWDFSLNPCDGNASWRTPLSKNQSSQIFNNVVNCTCSPDDACHVVNISLKGQDLAGVLPPSLAKLPSIKMIDLSYNYLSGPIPHEWQSTELEFICLENNMLSGNVPAELGKLNKLNILVLTANNLTGELPMELNNLTNLTELRLDSNYFSGRIPNLGSLTQLQKLEIRGSGLEGPIPESISLLTNLSRLAISDLNGEGSQVFPNLSSMTKMNKLMLRGCRLTGSIPGYISQMSKLQYLDLSFNDLGGDIPDLSGLNNLEKMYLTGNSLNGNVPSWLTNQINNYRQVDLSYNHLNETTVPKECIEPLNLFRSYTSSEDSDLGKCLNRFPCLKDYYSVHINCGGQRVTIGDKVFEADIDLSGAARFLPSNDHWGISNTGRLVAHHCLSLTMVVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C7X1</accession>
    <name>A0AAD5C7X1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">K-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_000483</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009257">
        <property type="protein sequence ID" value="KAI7736499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C7X1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002487">
        <property type="entry name" value="TF_Kbox"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01486">
        <property type="entry name" value="K-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51297">
        <property type="entry name" value="K_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="K-box" evidence="3">
        <location>
            <begin position="26"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="114"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="26"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7736499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="167" mass="19165" checksum="17049E30D6704F39" modified="2024-05-29" version="1" fragment="single">VKGTIDRYKKACLDPPSSGSVAEANAQFYQQEAAKLRQQIANLQNQNRNIMGESLGNMQAKDLKNLESKLEKGISRIRSKKNELLFAEIEYMQKRENELHNNNQFLRAKIAENERSQQQHMSLMPGSSDYDLVPPHQPFDGRNYLQVNELQPNNSYSCQDQTPLQLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C8M2</accession>
    <name>A0AAD5C8M2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_014283</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009371">
        <property type="protein sequence ID" value="KAI7736099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C8M2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7736099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="118" mass="13463" checksum="D3F209E2C3CA1A01" modified="2024-05-29" version="1" fragment="single">MQELEEALGLYKLVETLQLQQFKEACEMHELEEALGLHKLVETLQQHEFEETLKLQSDVPQVSEKLIGDLVFKIGASRIDYEVLKAIYDMPDTPLSYIASDQIYSHLSRGILVDNGNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C9G3</accession>
    <name>A0AAD5C9G3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">M8C21_011968</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">AA19_3_7</strain>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="JAMZMK010008956">
        <property type="protein sequence ID" value="KAI7737793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C9G3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048544">
        <property type="term" value="P:recognition of pollen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00028">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01098">
        <property type="entry name" value="PAN_AP_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.90.10.10">
        <property type="entry name" value="Bulb-type lectin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.4.10">
        <property type="entry name" value="Hepatocyte Growth Factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001480">
        <property type="entry name" value="Bulb-type_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036426">
        <property type="entry name" value="Bulb-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000858">
        <property type="entry name" value="S_locus_glycoprot_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024171">
        <property type="entry name" value="SRK-like_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32444">
        <property type="entry name" value="BULB-TYPE LECTIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32444:SF98">
        <property type="entry name" value="RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01453">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08276">
        <property type="entry name" value="PAN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00954">
        <property type="entry name" value="S_locus_glycop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000641">
        <property type="entry name" value="SRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00108">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00473">
        <property type="entry name" value="PAN_AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51110">
        <property type="entry name" value="alpha-D-mannose-specific plant lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50927">
        <property type="entry name" value="BULB_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50948">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Bulb-type lectin" evidence="15">
        <location>
            <begin position="117"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="14">
        <location>
            <begin position="337"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="16">
        <location>
            <begin position="392"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="492"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="672"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="687"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="17">
        <location>
            <position position="698"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAI7737793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="698" mass="78371" checksum="47E8BCC1B19024B0" modified="2024-05-29" version="1" fragment="single">RRNYHHQRRRFHHNRLSLSRKKPSDHYQCSVILETQPDQVETRLPVGVTVTIVLLHQLSYSDGNLKKEKGRNRALSSLTLQPPYHNHITTTSMTKHCRSPAALSSYVSLITVSPRFSHMQWSKRLTKAGRSMTTEAHHHHTMDHLRPPNSTNHYVGIWYKKISTGTVVWVANRNSPLTDTYGELTLTLQGVLILRDATKGNVVWSSANSSKTLMRNPIGHLLDTGNFIIYEEGGDHNQENPIWQSFDFVTDTLLPGMKYGRDFVTGIERHYTSWRSKEDPASGEFALSIDIRGYPQSILTQGQQITYRVGPWNGVRGKFTTNTIVSRQEWNLYLTPEIDQCDRYAICGPYASCNIEKSPVCECLKGFTPTSPDQWRVADWSQGCRHTVPLDCDPEEGFNKYSNMKLPNTQGSWYNLTMTLVECEQMCKTNCSCTAYANSNISGNGCLLWFGGLIDIRTVAEDGDTIYIRLSASELGTLKQLSQQKTVKVQVQGDDQYEKKQHQQDGSGDEDLELPLFGLSTVLKATNNFSMDNKLGEGGFGPVYKLISYINLILHSGYMAPEYAGGGIFSIKSDVYSFGVLVLEIVCGEKNKGFVHKEHCNNLIGHAWVLYNENRSLELVAKCLGESINVSQVLRSIHVALLCVQRHPEDRPTMTSVIVMLASQGPLPSPKEPGFCVGKTTQDNTQSSSSREISSTMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C9I5</accession>
    <name>A0AAD5C9I5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Polygalacturonase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_033689</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the glycosyl hydrolase 28 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009111">
        <property type="protein sequence ID" value="KAI7737123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C9I5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004650">
        <property type="term" value="F:polygalacturonase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.10:FF:000004">
        <property type="entry name" value="Pectin lyase-like superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000743">
        <property type="entry name" value="Glyco_hydro_28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31375">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00295">
        <property type="entry name" value="Glyco_hydro_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00710">
        <property type="entry name" value="PbH1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00502">
        <property type="entry name" value="POLYGALACTURONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0134">Cell wall</keyword>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6 9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <feature type="active site" evidence="8">
        <location>
            <position position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7737123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="412" mass="43882" checksum="10703D7EE52A9D6E" modified="2024-05-29" version="1">MNVAIDHRRMSLPLAFGAGRSHIVRLEPKLMKGTCGSVTAKVISTYNVVSLGAKANGRSDSKNAFLKAWNLACASTNPAIIYVPRGRYLIASAVTFAGQACKSKAITFKIDGTLVAPSSYNAIGNMEVWIKFHRVNHVTISGGTLDAQGSPLWACKSSRKTCPKGATSLGIYHSQNIVIKNLRSLNSQMFHILLYACNNAKIQGVSIVAPGQSPNTDGIHLTSSTGVTILNSKISTGDDCISIGPGNSNIWIEKVACGPGHGISIGSLGWDLQEPGVQNVTVKTTTFIGTQNGVRIKTWARRSNGFVKNVLFQHSTMVNVQNPIIIDANYCPNNKKCPNHVAGVKISNVVYEDVHGTSATRVAVKFDCTKGKPCSGIRLKDVNLKYGNRPAASSCSYVAGAVSGLRGFTSCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CB35</accession>
    <name>A0AAD5CB35_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nascent polypeptide-associated complex subunit alpha-like UBA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_001255</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009079">
        <property type="protein sequence ID" value="KAI7737226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CB35"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14361">
        <property type="entry name" value="UBA_HYPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052617">
        <property type="entry name" value="Huntingtin-int_K"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038922">
        <property type="entry name" value="HYPK_UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044034">
        <property type="entry name" value="NAC-like_UBA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31184:SF2">
        <property type="entry name" value="HUNTINGTIN-INTERACTING PROTEIN K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31184">
        <property type="entry name" value="HUNTINGTIN-INTERACTING PROTEIN K FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19026">
        <property type="entry name" value="UBA_HYPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nascent polypeptide-associated complex subunit alpha-like UBA" evidence="2">
        <location>
            <begin position="67"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7737226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="112" mass="12455" checksum="B40D35D9535B49A2" modified="2024-05-29" version="1">MEGGDEERVEDSKDLQQQSKALDKLTDHVEDRQLDSTRVQEAMASIAASKEADLNAMRLREKELAAVKINAAEVDIIANELELDKKVAERTLREHKGDAVAAIRHLLHVGTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CBC8</accession>
    <name>A0AAD5CBC8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_012977</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="JAMZMK010008835">
        <property type="protein sequence ID" value="KAI7738314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CBC8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000060">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002:SF1086">
        <property type="entry name" value="BULB-TYPE LECTIN DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002">
        <property type="entry name" value="RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE SD1-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="1"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7738314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="212" mass="23820" checksum="98707E583B45235F" modified="2024-05-29" version="1">MRFNIIMGIARGLNYLHHDSRLRIIHRDLKTSNILLDEEMNPKISDFGLAKIVKGNDMEAISNKVVGTFGYMSPEYARDGKFSDKSDVFSFGVVLLEILSGKRSTGFYHSQNSISLLGHAWNLWIENRPFELLDQIIMISYNSSEVLKCITVGLLCVQGNPNDRPTMTNVVMMLGGDTTTLPSPKEPPFIPRRDNTTIPSNNMLTITEVIGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CBS4</accession>
    <name>A0AAD5CBS4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein root UVB sensitive 1, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_018608</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RUS1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008730">
        <property type="protein sequence ID" value="KAI7738757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CBS4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009941">
        <property type="term" value="C:chloroplast envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032502">
        <property type="term" value="P:developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010224">
        <property type="term" value="P:response to UV-B"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006968">
        <property type="entry name" value="RUS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055412">
        <property type="entry name" value="UVB_sens_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054549">
        <property type="entry name" value="UVB_sens_RUS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12770:SF22">
        <property type="entry name" value="PROTEIN ROOT UVB SENSITIVE 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12770">
        <property type="entry name" value="RUS1 FAMILY PROTEIN C16ORF58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24160">
        <property type="entry name" value="UVB_sens_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04884">
        <property type="entry name" value="UVB_sens_prot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein root UVB sensitive/RUS" evidence="3">
        <location>
            <begin position="167"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="Root UVB sensitive protein C-terminal" evidence="4">
        <location>
            <begin position="376"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="27"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="34"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="508"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF24160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7738757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="508" mass="55718" checksum="41BED5320A7E413E" modified="2024-05-29" version="1" fragment="single">SNRLTSATKQNKPSSPLSLAVAAPFTVDHHHPTDAPPLPPPHLPPPHHLLHRHTPKNLIPSSSSSSHMETITFPFTFPHNPSQIIPLPTLFRAYAIDTGNEIGTNNDDEVIVWEVKGGKRTKLVGDNVLDMFVVPSVRWAWWDLQSGSKEDSSTTVSLGNVVPTMWVQCRELFMRLMLPEGYPESVTSDYMEYSCWRGVQGVAAQVSGVLATQSLLYAVGLGKGAIPTAAAVNWVLKDGIGYLSKIFLSKFGRHFDVNPKGWRLFADFMENAAFGMEILTPAFPHLFVPIGAAAGAGRSAATLIQAATRSCFFAGFAAQRNFAESFVGLVPSVKEVNDEEPFFPALPLLSLKPASKVEHNLLSPEAKEAAAIIDQRLELGSKLSEIVKTKTDAHALLDLYKNQGYILTEQNGKFCVILKDTCSPQDMLKSMFHVSYLYWLEKNVGLKPVSIYDDCRPGGMLQISLEYVEREFNHAKHDGELAGIGEIEKVFVSVNVVTCEDAPLASCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CDY0</accession>
    <name>A0AAD5CDY0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_011725</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008714">
        <property type="protein sequence ID" value="KAI7738844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CDY0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="66"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7738844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="90" mass="10088" checksum="3E44B25BF47521EC" modified="2024-05-29" version="1">MAPKAEKKPAEKKPAEDKAPAEKKPKAGKKLPKEAGSAAVDKKKKRHKKSVETYKIYIFKVLKQNAYLAVAMGVLCNYYYAFSMLVLQLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CEA2</accession>
    <name>A0AAD5CEA2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_020067</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008696">
        <property type="protein sequence ID" value="KAI7738906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CEA2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11017">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11017:SF340">
        <property type="entry name" value="NB-ARC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7738906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="578" mass="66865" checksum="FF8F2C361C8B22E3" modified="2024-05-29" version="1">MEYDRIEAIQYHPDHHDHDHSSHLCKIVSNMKKLRYLSVTILDRKNDTTEGPNFLSNELRYIRWENYPKSPLPDTFQPMNLVDLKLSKSLQKVLWKGYKHLPRLKVLELYHMKNLLSTPDFCGLPCLRKLTLCNCEELKEIHPSLGCHMGLEYVSILDCYKLKKFPTIIRMRNLGTLEIQFCNDTLEFPEIQSNMESLVKLSLRYMRIDVLLSSIGERCVNLISLYLLNCTSLKSIEVDFDGLKHLEEFTLYGLNHLKTRNQLCISRLVSKVGCGKDWYTSHPSIRLWLTHSLTKLYLCDCGLRDGEIPSNIGELYNLQELHLGGNDFTRLEISLLQLTRLKLLILDGCKELVELPELPSGLYILYADTCHRLKAIGDFHKHCKYLSGVSLLYGSFIIGGARLLETMLQGNAIEHHSMMLCLQGIEIAKGFIPPLLRASKCTLKLPENWCNDFCGFLICFISYRKILKWRHITLSMEHVMNDIDSQEDMVWDESDNDMYTRVWYVSFGSLRHTAWWDETKNMVSFSINMICGGFGVRPVPWRSGSSPTQTSAHYDGESRYAPKFRFLHDSAHGLDISF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CFV6</accession>
    <name>A0AAD5CFV6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cell cycle checkpoint control protein RAD9A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_015980</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the rad9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008226">
        <property type="protein sequence ID" value="KAI7741273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CFV6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030896">
        <property type="term" value="C:checkpoint clamp complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071479">
        <property type="term" value="P:cellular response to ionizing radiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000076">
        <property type="term" value="P:DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031573">
        <property type="term" value="P:mitotic intra-S DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.70.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026584">
        <property type="entry name" value="Rad9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007268">
        <property type="entry name" value="Rad9/Ddc1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15237:SF0">
        <property type="entry name" value="CELL CYCLE CHECKPOINT CONTROL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15237">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04139">
        <property type="entry name" value="Rad9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009303">
        <property type="entry name" value="Cell_cycle_RAD9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="246"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="353"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="246"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="368"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR009303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7741273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="392" mass="43381" checksum="A8DDF1FEEFF901A6" modified="2024-05-29" version="1">MEWSVSGNALKTLGRCVTCLARIGNDLTLQASSSQAICAVLRTPLTSIDHLSVILPDPDASKVQWTLDCFNGMKKSYWITCNVEANVQQLSLDRRRLPSSFVVRPRDLNRLLSNFQSSLQEITVIATEPTSDDEVGGKAVELRSYIDPAKENDSSLHTQLWIDPSEEFVQYKHTGDPVDVTFGVKELKAFLSFCEGCEVDIHLYFEKAGEPILMAPKFIEDASVSNFDATLVLATMLVSQLHGTTTTSEPVPAAAAAATAPPKDNVSEHPSDHTRIWSELSASATKSANPSARVHVERDLNTNDQGDIQRIRTMHISKDGPDGRNFNTNACHPIPMETEHEHVDEASDRRAEINDHAVVSQHHLSNWVDDDDNDEEDDDSEPYVQATPPYCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CGI3</accession>
    <name>A0AAD5CGI3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">WRKY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_010322</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008511">
        <property type="protein sequence ID" value="KAI7740104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CGI3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.80:FF:000004">
        <property type="entry name" value="WRKY transcription factor 65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.80">
        <property type="entry name" value="WRKY domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003657">
        <property type="entry name" value="WRKY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036576">
        <property type="entry name" value="WRKY_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044810">
        <property type="entry name" value="WRKY_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32096:SF104">
        <property type="entry name" value="WRKY DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32096">
        <property type="entry name" value="WRKY TRANSCRIPTION FACTOR 30-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03106">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00774">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118290">
        <property type="entry name" value="WRKY DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50811">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="WRKY" evidence="7">
        <location>
            <begin position="167"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="223"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="225"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="238"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7740104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="340" mass="37834" checksum="64A60424896A9BBA" modified="2024-05-29" version="1">MDVDWDLQAVVRGCCSATATTEPPESCTQQKSTFYEDNNNNNNIDVLTFSEDLFQPRNDINSIEKFMNDLYNCNPLQFTNLQQQQQTLPHQSLPISGFSFLGEHQEPPYNHHYHHHQQQVKQFQVKQQALGISRCTTTHAHGAKKIKKRKNQNKKICQVPAEGSPPDLWSWRKYGQKPIKGSPYPRGYYKCSTLKGCLARKQVERNRSDPGMLIITYTGEHTHPVPTQVNSLSDSIRNKTTTSGDGDTGNNPTPSPTSSPVASLPSVMDKTDDIDDDNNFDISGLVVDGETFDGFDDLVGSAVGDMFSDHCAAATADPTFPWLPNNLPPQPPLLPAGIDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CHP5</accession>
    <name>A0AAD5CHP5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_023532</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008361">
        <property type="protein sequence ID" value="KAI7740721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CHP5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7740721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="98" mass="10965" checksum="7CF7D1E77102914A" modified="2024-05-29" version="1">MVYIKQKDENNKQKKERIVHDSRGQNRIKSSGSRLLINTENELTISGLKLSIISAVIAIYALCPPPLQPPIDDAFVANPMLSLQQLVKESQLLPGNRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CIQ7</accession>
    <name>A0AAD5CIQ7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_012390</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007890">
        <property type="protein sequence ID" value="KAI7742803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CIQ7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7742803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="85" mass="9925" checksum="537C614D4649F7B0" modified="2024-05-29" version="1">MLWCSDSIKKRLRILCVRALEKATLMFLTSSWPLELLAFQVDYMLGDNPRATRLHGWIWRSYQQLHHRGSLIVSIKDSYSGKASE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CL84</accession>
    <name>A0AAD5CL84_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycine-rich protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_031335</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007857">
        <property type="protein sequence ID" value="KAI7742910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CL84"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37736">
        <property type="entry name" value="GLYCINE-RICH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37736:SF1">
        <property type="entry name" value="GLYCINE-RICH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="284"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="90"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="320"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="354"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="410"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7742910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="438" mass="47833" checksum="E7CF7E976D93DA32" modified="2024-05-29" version="1">MASATASSDVTDGPVLTLLNKRIRNLKKKLNRITQLEDSVSKGKSVIKNKEQEDLLKSKPSIISAVDELEKLRQPLSSAVDEEIALAVKQSQSQSQSESQTLTSENNVTDVADGNGVNDGVNNGVNELIEDLLKVIYFGSMFEVKSQSEFNAIMLTKTHERNCCLTYDYVTDDDAAVMLGETDLDLISKMSSLLVSRPVDSSFSHQNALQKCVEHAKLWVAKSDQPIDSNSEVTYASLREKLTKIIGSDYLKITPEMKATSDVAAEAAGNYGFQVPVQVESTDAPHEQLEDNSTDFQRNETQEDQSIPVEDSHEHEQEAENSIETQVQAEQGESNAAGSEYKEQYVPRRYHNQRGGGRNNGNGGRSGGRGYGGGRGGRSGGRGGFYQNGRNQYHEQPGNYYPSNQRNYYGGRGRGGRGNGGNGNGYNQHASGVEVAES</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CLK1</accession>
    <name>A0AAD5CLK1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="26" type="ORF">M8C21_032734</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="26">AA19_3_7</strain>
            <tissue evidence="26">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the leguminous lectin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="26">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="EMBL" id="JAMZMK010007548">
        <property type="protein sequence ID" value="KAI7744318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CLK1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002229">
        <property type="term" value="P:defense response to oomycetes"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000240">
        <property type="entry name" value="Lectin-domain containing receptor kinase A4.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000041">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.430:FF:000004">
        <property type="entry name" value="Putative leucine-rich repeat receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.430">
        <property type="entry name" value="Galactose-binding lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051824">
        <property type="entry name" value="LRR_Rcpt-Like_S/T_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021720">
        <property type="entry name" value="Malectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48006">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN DDB_G0281931-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48006:SF81">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11721">
        <property type="entry name" value="Malectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 22" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="17 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="13 22" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-0675">Receptor</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="11 24" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="10 23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16 23" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="24">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041924771" description="non-specific serine/threonine protein kinase" evidence="24">
        <location>
            <begin position="17"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="583"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="767"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="25">
        <location>
            <begin position="540"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="672"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="26">
        <location>
            <position position="905"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="EMBL" id="KAI7744318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="905" mass="98934" checksum="BE853B670F1A2EBC" modified="2024-05-29" version="1" fragment="single">MLLVILCVTFLPFAFASINTTCLPSEEVDVLREIGRKLGKDWDFELDPCVEWSDSINNKTYVNNVTCNTEPNNTNICHVTSISLKGQSLAGILPPEFTNLPNLLNLDLARNYLGGTIPPSWGSMQQILNITLIGNRINGSIPKELGNLRTLTTLTVEDNMMSGIIPEELGNLASIERLFLNSNSFSGRLPASFANLTTIKEFRIGGNNFSGKIPDYIEQWKSLESLRIQASGLDGPIPSSITLLESLTDLRISDLGGPAALCPPFGNASFKNLILRNCNLFGNLPDSLPSGALKVLDFSFNKFNGTIPNGFMDLRPTDYIYLTGNLLSGIVPDWMLTSGDVIDLSYNNFTSQNSNNLRCQIRDTNLFASFSTDNTSKEVSCLGNNGVTDGNKEYEGDLDSGGASHFSSFANRWGFSNTGHFLDDDQPDSYILTNRSGVLVNNSELYMNARTSAISLTYYGFCMRNGSYNVSLHFAEIVFTDDEKFSSLGRRVFDIYIQGKLVEKDFDISARAGGVGKAIVTTYTIDVTTSLEIRLYWAGKGTINIPSRGNYGPLISAISVDPNFEVPLSDDSQGNGNGVSGGVVAGIVIGAVCAIILILGLLWWRGCLRCKDPELDLHGIELNGLELNTGSFTLKQIKAATDNFDVANKIGEGGFGSVYKGVLPNGTLIAVKQLSAKSKQGNREICIDIAKGLAFLHEESRLKIVHRDIKATNVLLDKDLNAKISDFGLAKLDEEDNTHISTRVAGTYGYMAPEYAMRGYLTDKADVYSYGIVLLEIVSGAIALKAEGKLMDLVDPKLDSEYDKQEVMVVISLALMCAAISPTERPTMSSVVSMLEGRIVPEAFSAEQSFSMTKTDRDKMMKLLEIKDENQPEEMSFAYTDSSTSAADLYPVNGVSEYLQKREVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CLV6</accession>
    <name>A0AAD5CLV6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Alcohol dehydrogenase class-3</fullName>
            <ecNumber evidence="6">1.1.1.1</ecNumber>
            <ecNumber evidence="5">1.1.1.284</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Alcohol dehydrogenase class-III</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Glutathione-dependent formaldehyde dehydrogenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">S-(hydroxymethyl)glutathione dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">M8C21_001751</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="23">AA19_3_7</strain>
            <tissue evidence="23">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>S-(hydroxymethyl)glutathione + NAD(+) = S-formylglutathione + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19985"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57688"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58758"/>
            <dbReference type="EC" id="1.1.1.284"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>S-(hydroxymethyl)glutathione + NADP(+) = S-formylglutathione + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19981"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57688"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58758"/>
            <dbReference type="EC" id="1.1.1.284"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>a primary alcohol + NAD(+) = an aldehyde + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10736"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15734"/>
            <dbReference type="ChEBI" id="CHEBI:17478"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>a secondary alcohol + NAD(+) = a ketone + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10740"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17087"/>
            <dbReference type="ChEBI" id="CHEBI:35681"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 20">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.1" evidence="6"/>
    <dbReference type="EC" id="1.1.1.284" evidence="5"/>
    <dbReference type="EMBL" id="JAMZMK010007617">
        <property type="protein sequence ID" value="KAI7743984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004022">
        <property type="term" value="F:alcohol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051903">
        <property type="term" value="F:S-(hydroxymethyl)glutathione dehydrogenase [NAD(P)+] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046294">
        <property type="term" value="P:formaldehyde catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08300">
        <property type="entry name" value="alcohol_DH_class_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000003">
        <property type="entry name" value="S-(hydroxymethyl)glutathione dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.180.10:FF:000001">
        <property type="entry name" value="S-(hydroxymethyl)glutathione dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014183">
        <property type="entry name" value="ADH_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43880">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43880:SF58">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE CLASS-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 20" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0520">NAD</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 20" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alcohol dehydrogenase-like N-terminal" evidence="22">
        <location>
            <begin position="34"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="Alcohol dehydrogenase-like C-terminal" evidence="21">
        <location>
            <begin position="200"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00020108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00033399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00049164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00049243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF00107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF08240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="KAI7743984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="355" mass="37916" checksum="2BBDDDD1951C8823" modified="2024-05-29" version="1">MATQGKIITCKAAVAYEANKPLVIEDVEVAPPQAGEVRLQILFTALCHTDAYTWSGKDPEGLFPCILGHEAAGIVESVGEGVTEVQPGDHVIPCYQAECKDCKFCKSAKTNLCGKIRGATGFGVMMSDRKSRFSVRGTPINHFMGTSTFSQYTVVHDVSVAKIDPKAPLEKVCLLGCGVPTGLGAVWNTAKVEAGSSVAIFGLGTVGLAVAEGAKAAAKKFGVTEFVNPKEHGKPIQQVLVEMTDGGVDYSFECIGNVSLMRAALECCHKGWGTAVIVGVAASGQEITTRPFXXXXXTAFGGFKSRSQVPQLVDKYLKKEIKIDEYITHKLTLEEINKAFELLHGGDCLRCVLKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CMF1</accession>
    <name>A0AAD5CMF1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cyanate hydratase</fullName>
            <shortName evidence="3">Cyanase</shortName>
            <ecNumber evidence="3">4.2.1.104</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Cyanate hydrolase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Cyanate lyase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">CYN</name>
        <name evidence="5" type="ORF">M8C21_016583</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 3">Catalyzes the reaction of cyanate with bicarbonate to produce ammonia and carbon dioxide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>cyanate + hydrogencarbonate + 3 H(+) = NH4(+) + 2 CO2</text>
            <dbReference type="Rhea" id="RHEA:11120"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29195"/>
            <dbReference type="EC" id="4.2.1.104"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cyanase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.104" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010007534">
        <property type="protein sequence ID" value="KAI7744452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CMF1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008824">
        <property type="term" value="F:cyanate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00559">
        <property type="entry name" value="Cyanase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1160.10:FF:000002">
        <property type="entry name" value="Cyanate hydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1160.10">
        <property type="entry name" value="Cyanate lyase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00535">
        <property type="entry name" value="Cyanate_hydrat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008076">
        <property type="entry name" value="Cyanase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003712">
        <property type="entry name" value="Cyanate_lyase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036581">
        <property type="entry name" value="Cyanate_lyase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00673">
        <property type="entry name" value="cynS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34186">
        <property type="entry name" value="CYANATE HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34186:SF2">
        <property type="entry name" value="CYANATE HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02560">
        <property type="entry name" value="Cyanate_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001263">
        <property type="entry name" value="Cyanate_hydratas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01693">
        <property type="entry name" value="CYANASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01116">
        <property type="entry name" value="Cyanate_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55234">
        <property type="entry name" value="Cyanase C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyanate lyase C-terminal" evidence="4">
        <location>
            <begin position="80"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="93"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="96"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7744452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="167" mass="18763" checksum="6B60401F45030EB1" modified="2024-05-29" version="1">MEPETPTKAHVVSSLMSVKKKSGKTFGEIASETGLTNVYVAQLLRRQAQLKPDTVPKLRASLPGLTDTHINQMMDFPLRSYDPSIVQDPTIYRLNEAVMHFGESIKEIINEEFGDGIMSAIDFFCSVDKVKGVDGKDRVVLTFDGKYLPHTEHKVEHMVSRLNLQKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CN73</accession>
    <name>A0AAD5CN73_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Gnk2-homologous domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_000022</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007378">
        <property type="protein sequence ID" value="KAI7745043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CN73"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23509">
        <property type="entry name" value="Gnk2-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.430.20">
        <property type="entry name" value="Gnk2 domain, C-X8-C-X2-C motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002902">
        <property type="entry name" value="GNK2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038408">
        <property type="entry name" value="GNK2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32099">
        <property type="entry name" value="CYSTEINE-RICH REPEAT SECRETORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32099:SF108">
        <property type="entry name" value="GNK2-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01657">
        <property type="entry name" value="Stress-antifung"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51473">
        <property type="entry name" value="GNK2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Gnk2-homologous" evidence="3">
        <location>
            <begin position="3"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="Gnk2-homologous" evidence="3">
        <location>
            <begin position="110"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7745043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="256" mass="28635" checksum="1E605C7EF593B990" modified="2024-05-29" version="1" fragment="single">GTTNLGYDCLQARNSTENTVYKSNLRSLLISLATNAPLQDGFFNTSVGNGSNRVYGLVWCRADASPDTCSKCLNDSISLSLRECPDSKHLVIWSSLCSIRFSNESFFGELWNMSSSSTYGNSGLDDPAVFMRGFSMMETLVRNVSSQPLMFATALIDVGENGERYGLGQCSRDLSKLDCENCFEKLLTSYQDYVLNRTGWEMLGVSCGMWYDNFLFYDNNSSRPNPTPNTSGGERWYNQGDMILAFVAMLVLYWIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CPS4</accession>
    <name>A0AAD5CPS4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">M8C21_007289</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">AA19_3_7</strain>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010007093">
        <property type="protein sequence ID" value="KAI7745868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CPS4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000004">
        <property type="entry name" value="Calcium-dependent protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000086">
        <property type="entry name" value="calcium-dependent protein kinase SK5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="28"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="18">
        <location>
            <begin position="174"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="18">
        <location>
            <begin position="210"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="18">
        <location>
            <begin position="246"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="18">
        <location>
            <begin position="282"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="7"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAI7745868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="343" mass="38964" checksum="34602BAB6A449A48" modified="2024-05-29" version="1">MKKHTKNSSLKPSNTVLSHPTPRLRDHFIMGKKLGHGQFGTTYLCRQKSTGVDFACKSIPKRKLLCKEDYDDVWREIQIMHHLSEFPCVVRIKGAYEDNVFVHLVMELCAGGELFDRIIQKGHYSEKEAAKLMKTIVGVFSSTSHTPFVLPNIYSITAHELLDQIVIAERLSEEEIGGLKQLFKMLDTDNSGTITFEELKQGLKKVGSNLTESEIKELMDAADIDSNGTIEYGEFLAATLHMNKLEREENLLAAFRFFDKDGSGYITRDELQQASKDFGLQDAQLDEMIREVDQDNDGRIDYAEFTAMMRKGDDEVRSRAMKGNLNFDLAEALSFNKNKESQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CR68</accession>
    <name>A0AAD5CR68_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_005259</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007392">
        <property type="protein sequence ID" value="KAI7745011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CR68"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7745011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="107" mass="12197" checksum="5CD437820959C3E2" modified="2024-05-29" version="1" fragment="single">PFLLYFLLQTVTYTPDLCLQGRFCAWNLLIKSMAMVDSPLPSRLKELSQEAFIYICIVSIRTGKGHHKWAYVEVHGIHMSTIHVPDPNSLRPALANLQFKEKDTGPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CVB4</accession>
    <name>A0AAD5CVB4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glucan endo-1,3-beta-D-glucosidase</fullName>
            <ecNumber evidence="3">3.2.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_007926</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of (1-&gt;3)-beta-D-glucosidic linkages in (1-&gt;3)-beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.39"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the glycosyl hydrolase 17 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.39" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010006817">
        <property type="protein sequence ID" value="KAI7747201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CVB4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042973">
        <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000005">
        <property type="entry name" value="Glucan endo-1,3-beta-glucosidase 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000490">
        <property type="entry name" value="Glyco_hydro_17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044965">
        <property type="entry name" value="Glyco_hydro_17_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32227">
        <property type="entry name" value="GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00332">
        <property type="entry name" value="Glyco_hydro_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="315"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7747201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="334" mass="37268" checksum="BF0BB9EC15BD3928" modified="2024-05-29" version="1">MKAAKIKNTRIYDANHEVLKAFKGSGIEIIIGLGNEFLKDISVSQDRAMDWVKQNVQPFVPATRIKGIAVGNEVLGGADQELWEVLLPAVKNIHNALDLLHLADDVEVSSPHSAGVFASSFPPSAGAFKESLMPYMKPLLEFFSRIKSPFYINTYPFLAYISDPEHIDINYATFKKNPGIYDEKTKLHYYNMFEAQIDATYAALEKAGFDKMEVIVSETGWASKGDPNEAGATVENARTYNLKLRKRLLKKKGTPYRPKMVVKAYVFAMFNENLKPGPTSERNFGLFKADGSIAYDIGFTGLVPSAATSVFSFKACNKLIIFLLNVFIFYILKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-02-05" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CVD0</accession>
    <name>A0AAD5CVD0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_012104</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006565">
        <property type="protein sequence ID" value="KAI7748297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33144:SF50">
        <property type="entry name" value="OS03G0714750 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33144">
        <property type="entry name" value="OS10G0409366 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7748297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="314" mass="36382" checksum="193AFE0A33DD6B04" modified="2024-05-29" version="1" fragment="single">SEKHXXXXQNTHARDVDFIPDLSDNSEEDYQQVDRSVVVSKKQHRRQYISPMSMNRIANLTKIRRVVPPNVSEKVSSTSIVTKTNKRPSITMDEISKGNKGVKRSMALVDEEDDEFDEIFQEGNRVDMEVNDVDQDDEYEDMDDVIYTNNRHEIQMEDHDSSKQKDNIEEQLQGPTIQNATKSISRRKRLHITSIVSNEHIDDRVDDDHVEDNVEEHQVFETQGHQTSEIQGASKKVRGYTQKAETWKMDSTQRIVVTFNKFGKPIGDEGNELVQFLGTLVRMGNNVSIEYSDWRKVPKQKKEDMYSLVKVYSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CWX8</accession>
    <name>A0AAD5CWX8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">VPS37 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_000252</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VPS37 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006361">
        <property type="protein sequence ID" value="KAI7749432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CWX8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000813">
        <property type="term" value="C:ESCRT I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043162">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.660">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037202">
        <property type="entry name" value="ESCRT_assembly_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029012">
        <property type="entry name" value="Helix_hairpin_bin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009851">
        <property type="entry name" value="Mod_r"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13678">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13678:SF2">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 37A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07200">
        <property type="entry name" value="Mod_r"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140111">
        <property type="entry name" value="Endosomal sorting complex assembly domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51314">
        <property type="entry name" value="VPS37_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="5 6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="VPS37 C-terminal" evidence="8">
        <location>
            <begin position="12"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="8"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7749432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="93" mass="10609" checksum="D1D55C259675B953" modified="2024-05-29" version="1">MIIRTSELASAQEKLNDLTKQKAEILKSYSPGSLLHKLQESMDKTDEESETLHQQLLDKEIDLATFVQKYKKLRVVYHKRALTHLAAKTSVVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CXQ0</accession>
    <name>A0AAD5CXQ0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">glucose-1-phosphate adenylyltransferase</fullName>
            <ecNumber evidence="5">2.7.7.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_031903</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>alpha-D-glucose 1-phosphate + ATP + H(+) = ADP-alpha-D-glucose + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:12120"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57498"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="EC" id="2.7.7.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Glycan biosynthesis; starch biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Heterotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the bacterial/plant glucose-1-phosphate adenylyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.27" evidence="5"/>
    <dbReference type="EMBL" id="JAMZMK010006380">
        <property type="protein sequence ID" value="KAI7749362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008878">
        <property type="term" value="F:glucose-1-phosphate adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005978">
        <property type="term" value="P:glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019252">
        <property type="term" value="P:starch biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04651">
        <property type="entry name" value="LbH_G1P_AT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.10.10:FF:000010">
        <property type="entry name" value="Glucose-1-phosphate adenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011831">
        <property type="entry name" value="ADP-Glc_PPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005836">
        <property type="entry name" value="ADP_Glu_pyroP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43523:SF4">
        <property type="entry name" value="GLUCOSE-1-PHOSPHATE ADENYLYLTRANSFERASE LARGE SUBUNIT 3, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43523">
        <property type="entry name" value="GLUCOSE-1-PHOSPHATE ADENYLYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25247">
        <property type="entry name" value="LbH_GLGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="Trimeric LpxA-like enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00808">
        <property type="entry name" value="ADP_GLC_PYROPHOSPH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00809">
        <property type="entry name" value="ADP_GLC_PYROPHOSPH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00810">
        <property type="entry name" value="ADP_GLC_PYROPHOSPH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0750">Starch biosynthesis</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleotidyl transferase" evidence="12">
        <location>
            <begin position="94"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleotidyl transferase" evidence="12">
        <location>
            <begin position="291"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7749362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="587" mass="65154" checksum="53111944E7B18EF4" modified="2024-05-29" version="1">MDYCCSMINKGTLLPGGDGGLRINEGFLKGGLNGTVWVNNVHKRLGFQKQVRKITKPGVAFSLITSDSGKETLVQTLTAPKFERRRIDPKNVAAIILGGGAGTQLFPLTSKAATPAVPVGGCYRLIDIPMSNCINSSINKIFVLTQFNSASLNRHISRTYFGNGVNFGDGFVEVLAATQTSGETGMNWFQGTADAVRQFTWVFEVRVCIYFILLIEYTLKELQFYVFISALCSVTSCXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXHIFEMYKQNHIDRDADITLSCVPVGESRASDFGLLKFDHKGKVVQFAEKPTGEDLHAMRVDTTLLGLSQQEAEESPYIASMGIYVFKKDVLFKLLRWRYPTSNDFGSEILPAAVTEHNVQAYLFRDYWEDIGTIKSFYDANLALTDEVPKFQFYDPKTPFFTSPRFLPPSKIEKCKIKNAIISHGCFLRECSIEHSIVGERSRIDSGVELKDTLMLGADYYQTESEIASLLANGKVPIGIGSNTKIRNCIIDKNAKIGSDVVIMNKDGVEEGDRADEGFYIRSGITIILEKATIKDGVVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D072</accession>
    <name>A0AAD5D072_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CobW/HypB/UreG nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_033254</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005870">
        <property type="protein sequence ID" value="KAI7751613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D072"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03112">
        <property type="entry name" value="CobW-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003495">
        <property type="entry name" value="CobW/HypB/UreG_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051316">
        <property type="entry name" value="Zinc-reg_GTPase_activator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13748">
        <property type="entry name" value="COBW-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13748:SF40">
        <property type="entry name" value="OS04G0599700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02492">
        <property type="entry name" value="cobW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CobW/HypB/UreG nucleotide-binding" evidence="1">
        <location>
            <begin position="78"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7751613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="259" mass="28582" checksum="C4E0F57CC95C3572" modified="2024-05-29" version="1">MATVLRRVSLKTISSLQTSLQHTGLFYPTTNTPVLSQFITRTLQSIAFLNPYPRNMCTGATYQPHYEAAALNLDNRVPATIITGFLGSGKTTLLNHILTAQHGKRIAVIENEFGEVDIDGSLVASHSSANEDIVMVNNGCLCCTVRGELVTMLLELVKKRRDKFDHIVIETTGLAKPGPVIETFCTDEQISRYVKLDGVVTLVDSKHALQHLNEVKPRFVVNEAVEQVAYADRIILNKIDLVSDADLEALAKKIQNPKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D2J6</accession>
    <name>A0AAD5D2J6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">[RNA-polymerase]-subunit kinase</fullName>
            <ecNumber evidence="1">2.7.11.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_013042</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.23" evidence="1"/>
    <dbReference type="EMBL" id="JAMZMK010006093">
        <property type="protein sequence ID" value="KAI7750740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D2J6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070985">
        <property type="term" value="C:transcription factor TFIIK complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008353">
        <property type="term" value="F:RNA polymerase II CTD heptapeptide repeat kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000624">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF0">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="1"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="157"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="161"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="175"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7750740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="275" mass="30986" checksum="569CA3D2F21D6E3A" modified="2024-05-29" version="1">MKPNNLLIGPRGQLKLADFGLARIFGSPDRRFTHQVFARWYRAPELLFGAKQYGPGVDVWAAACIFAELLLRRPFLQGSSDIDQLGKIFAAFGTPKASQWPDMIYLPDYVEYQHVPGQSFKTLFPMASDDALDLLSKMFAYDPKARISAQQALEHRYFSSGPPPTEPALLPRPPPKKESIESKPSEFGPTVLSPTRKSRRVMPHPEGYDGNIFQKEKVDDRGIGNERSGPAPMSLDFSVFEARPPARPTINSADRTHLKRKLDLEFQIPEEEDED</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-10-08" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D2X1</accession>
    <name>A0AAD5D2X1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ribosomal protein L2 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_012571</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005669">
        <property type="protein sequence ID" value="KAI7752474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000020">
        <property type="entry name" value="60S ribosomal protein L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.950.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000006">
        <property type="entry name" value="60S ribosomal protein L8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.950.10">
        <property type="entry name" value="Ribosomal protein L2, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002171">
        <property type="entry name" value="Ribosomal_uL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022669">
        <property type="entry name" value="Ribosomal_uL2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022671">
        <property type="entry name" value="Ribosomal_uL2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014726">
        <property type="entry name" value="Ribosomal_uL2_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022666">
        <property type="entry name" value="Ribosomal_uL2_RNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13691:SF16">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13691">
        <property type="entry name" value="RIBOSOMAL PROTEIN L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03947">
        <property type="entry name" value="Ribosomal_L2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01383">
        <property type="entry name" value="Ribosomal_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01382">
        <property type="entry name" value="Ribosomal_L2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00467">
        <property type="entry name" value="RIBOSOMAL_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL2 RNA-binding" evidence="6">
        <location>
            <begin position="11"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL2 C-terminal" evidence="5">
        <location>
            <begin position="50"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="164"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7752474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="213" mass="22713" checksum="B176A6B11427407F" modified="2024-05-29" version="1">MGRVIRAQRKGAGSVFKSHTHHRKGPARFRSLDFGERNGYLKGVITEIIHDPGRGAPLAXXXLGAVVCNVEHHVGDRGSFARASGDYAIVISHNPDNGTTRVKLPSGAKKIVPSGCRAMIGQVAGGGRTEKPMLKAGNAYHKFRVKRNCWPKVRGVAMNPVEHPHGGGNHQHIGHASTVRRDAPPGQKVGLIAARRTGRLRGQAAATAAKADK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D352</accession>
    <name>A0AAD5D352_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_028106</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005468">
        <property type="protein sequence ID" value="KAI7753346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D352"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="84"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7753346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="113" mass="12612" checksum="3E4DDA384A3E3F63" modified="2024-05-29" version="1">MYAEGEARRSTCLHMLVCIDANWLLPNRRLQTLISVILHLQVRSVRETMNKMIEAWNAIPDVPQENGLAAPCFTTTMFHELASDVGPNERSPNLDENSASRTLCSSGTSILKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D3F7</accession>
    <name>A0AAD5D3F7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Mitochondrial carrier protein MTM1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_016994</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005657">
        <property type="protein sequence ID" value="KAI7752582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D3F7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990542">
        <property type="term" value="P:mitochondrial transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045315">
        <property type="entry name" value="Mtm1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45760">
        <property type="entry name" value="FI19922P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45760:SF6">
        <property type="entry name" value="MITOCHONDRIAL SUBSTRATE CARRIER FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="22"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="112"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="225"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7752582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="324" mass="35675" checksum="6CF006A1FD78CFDE" modified="2024-05-29" version="1" fragment="single">TRLQAQAAGVPYHNLYSTCHLETNTMLADARCFPSCYRSLVGEPPCSPQSFQYKGSLDVFRKVIQQEGFARLWRGTNASLALAMPTVGIYMPCYDIFRNYMEEYTNKNAPSMTPYVPLVAGSVARSLACVTCYPVELARTRMQAFKDGKHGTKLPGVFKTLVGVVYVDTSTKKLPSSFSSFRFLWTGLGAQLSRDVPFSAVCWATLEPVRRTLLRMSGDKSSLAAILGANFTAGFVAGSLAGATTCPLDVAKTRRQIEKDRIRALTMTTRQTLVEIWRDGGIKGLFTGVGPRVARAGPSVGIVVSFYEVVKYALHNRRHQPCKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D3Z6</accession>
    <name>A0AAD5D3Z6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_031987</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005258">
        <property type="protein sequence ID" value="KAI7753833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D3Z6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901259">
        <property type="term" value="P:chloroplast rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd21608">
        <property type="entry name" value="RRM2_NsCP33_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050502">
        <property type="entry name" value="Euk_RNA-bind_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048289">
        <property type="entry name" value="RRM2_NsCP33-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025">
        <property type="entry name" value="OS02G0815200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025:SF1">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="3" id="KW-0934">Plastid</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="6 8" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="94"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="192"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7753833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="266" mass="29729" checksum="C6B598AABCCA65F5" modified="2024-05-29" version="1" fragment="single">YLVCNTLKFLKPHKPMASSSFHFLHLPSSKTPSSSFISQTLLPSSSLHTHQTSLSIPTSINLTPSSQFFTKVAAVSHNLELKDEELEVGFSPQLKLFVGNLPFHVNNTSLALLFQQSGHVQAAEVVYDKLNRRSRGFAFVKMSSVQEAEAAAKKFDGYELDGRQLKVNYGPPPRKKPFIKKPRDLEVSENNNVVYVGNLSWNVDNETLETLFWEHGNVMQAKVFYDKGSGRSRGFGFVTYSCADEVYSAVKSLDGVDLDGRNIQVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D4C6</accession>
    <name>A0AAD5D4C6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_016539</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005591">
        <property type="protein sequence ID" value="KAI7752865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D4C6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.2700:FF:000001">
        <property type="entry name" value="Transmembrane protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056284">
        <property type="entry name" value="AIR9-like_A9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055474">
        <property type="entry name" value="DUF7046"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31149">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31149:SF7">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23080">
        <property type="entry name" value="DUF7046"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23197">
        <property type="entry name" value="IG_AIR9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIR9-like A9" evidence="3">
        <location>
            <begin position="223"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7046" evidence="2">
        <location>
            <begin position="317"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="96"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="126"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="127"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7752865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="406" mass="46236" checksum="287DE3822504F37C" modified="2024-05-29" version="1" fragment="single">ALDEKQNEAIESAANELARRKGVLDENLTLAHDLKVTEDERYIFMSSLVGLLAEYGILPRVTNAAALSDSIKLYYKRITKCIYLDKHLHDQMQQTIRESQERLSPSPYNNSTGGRHLESNYDVSKYPQLNGQMNQSQENDNRHKFEPRPVSQSIEHVYDNNRVNDRFQDGADNNYFQPPSVNDADDSFALEDGPGIEGFQIIGDAKPGGKLLGCGFPVRGTSLCMFQWVRHLQDGTREYIDGATNPEYVVTADDVDKLIAVECIPMDDQGRQGEIVRLFANEQNKITCDPEMQEEIDKYMSAGQASFNVSLLMDSSETWELTTFTLRRSNYQIKINRTQEIVIDEKYSSVLSIKIPSGLTSQFVLTRLNGSSHPFTFHDVRMRDTFVLTMRMFQSKALDERRKTKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D5Q5</accession>
    <name>A0AAD5D5Q5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_016968</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005657">
        <property type="protein sequence ID" value="KAI7752556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D5Q5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009658">
        <property type="term" value="P:chloroplast organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000373">
        <property type="term" value="P:Group II intron splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044190">
        <property type="entry name" value="THA8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47594">
        <property type="entry name" value="PPR CONTAINING PLANT-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47594:SF3">
        <property type="entry name" value="PROTEIN THYLAKOID ASSEMBLY 8, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7752556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="229" mass="25423" checksum="3BA3915A7444B414" modified="2024-05-29" version="1">MASSLRLHLPIHLPYNHPTANPRCYTPVRCGPRSNRGPLYKGRTLTIEAIQAVQSLKRTHRSDPSNNDAVFKTLSRLVKSDLIAAFNELIRQDQFDLAVKVFSVIKLEDWYKTDLNLYAKLVSAMASKGMMDEIDRLMNDVEVADVVSADGKGLVTVIKALLAADRAESTVRVYEMMKAGGWRCNSSADDYVGKVLSRGLRRLGKKEVADEIDAEIERVSGGVLEKMVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D5Z1</accession>
    <name>A0AAD5D5Z1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">GATA transcription factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_001767</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Transcriptional activator that specifically binds 5'-GATA-3' or 5'-GAT-3' motifs within gene promoters.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the type IV zinc-finger family. Class A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005431">
        <property type="protein sequence ID" value="KAI7753469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D5Z1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00202">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000018">
        <property type="entry name" value="GATA transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051140">
        <property type="entry name" value="GATA_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016679">
        <property type="entry name" value="TF_GATA_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45658">
        <property type="entry name" value="GATA TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45658:SF124">
        <property type="entry name" value="GATA TRANSCRIPTION FACTOR 5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00320">
        <property type="entry name" value="GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016992">
        <property type="entry name" value="TF_GATA_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00401">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00344">
        <property type="entry name" value="GATA_ZN_FINGER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0010">Activator</keyword>
    <keyword evidence="7 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 11" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GATA-type" evidence="13">
        <location>
            <begin position="198"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR016992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7753469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="310" mass="34618" checksum="C5B2F101A25EE690" modified="2024-05-29" version="1">MEEPNFFTGEFSYSHQFMIDDLLVDFPNHHDDVVLNDEFFDNVIGNSADSSTVVDSCNSSIACSEALFSGNVSSRSLSDSQFSGVELCVPYDDVAELEWLSTFTQDSFSTVDVQNLHIIPTGKKSPATDTSSTTTTTTTTVPYRINSQIFQNNVFVPGKARSKRSRTAPYDWTSRILHLKQPLPNKTTAFERREVQETSVVMKCLHCGSEKTPQWRTGPLGPKTLCNACGVRYKSGRLVPEYRPAASPTFVSAKHSNSHRKVMEIRRQKELRLAQKEFGNRGSGLVRSESNGGDDWLIDRQCGPGFVHMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D727</accession>
    <name>A0AAD5D727_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_029302</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000980">
        <property type="protein sequence ID" value="KAI7755596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D727"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7755596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="119" mass="13217" checksum="EAA0D9453AD53574" modified="2024-05-29" version="1">MLGSQAKGPSSGLNSSSDESHVMMICYLLCTRGQQWRESRDDDRKRREIRRKLVMENEGSGDILTSDRLISTVLQLSTPATETLMVYKGNASSGRGLVRADQCSCVEVMQGMELRCKAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D789</accession>
    <name>A0AAD5D789_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_023913</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010002904">
        <property type="protein sequence ID" value="KAI7754634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D789"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031072">
        <property type="term" value="F:heat shock protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10719">
        <property type="entry name" value="DnaJ_zf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000037">
        <property type="entry name" value="Chaperone protein dnaJ A6 chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.230.10:FF:000006">
        <property type="entry name" value="Chaperone protein dnaJ A6 chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.230.10">
        <property type="entry name" value="Heat shock protein DnaJ, cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.260.20">
        <property type="entry name" value="Urease metallochaperone UreE, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002939">
        <property type="entry name" value="DnaJ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008971">
        <property type="entry name" value="HSP40/DnaJ_pept-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001305">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036410">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43096">
        <property type="entry name" value="DNAJ HOMOLOG 1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43096:SF22">
        <property type="entry name" value="MOLECULAR CHAPERONE HSP40_DNAJ FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01556">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00684">
        <property type="entry name" value="DnaJ_CXXCXGXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57938">
        <property type="entry name" value="DnaJ/Hsp40 cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49493">
        <property type="entry name" value="HSP40/DnaJ peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51188">
        <property type="entry name" value="ZF_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="J" evidence="6">
        <location>
            <begin position="39"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="CR-type" evidence="7">
        <location>
            <begin position="173"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="CR-type" evidence="5">
        <location>
            <begin position="173"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7754634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="298" mass="32140" checksum="D5D6175B89B8AB19" modified="2024-05-29" version="1" fragment="single">GTLSFTRTKSCVSFSSSSQFSKKLYGKRRGGFVVLAAADYYSTLGVPKSATSKDIKAAYRKLARQYHPDVNKQPGATDKFKEISAAYEVLSDDKKRAMYDRYGEDGVKSTVGGQSGAYATNPFDLFETFFGTSMGGFAGGDATGFGGRRATTVIKGEDIRYDMRLEFSAAIFGTEKEFELSHLETCDACTGTGAKVGSKMRICSTCGGRGQVMRTEQTPFGMFSQVSVCPNCNGNGEMISEYCRKCSGEGRIRVKKDIKVKIPPGVSKGSILRVTGEGDAGPKGSVVKGVSNWFKDNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D7F0</accession>
    <name>A0AAD5D7F0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_024810</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in cellular auxin homeostasis by regulating auxin metabolism. Regulates intracellular auxin accumulation at the endoplasmic reticulum and thus auxin availability for nuclear auxin signaling.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the auxin efflux carrier (TC 2.A.69.2) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010003901">
        <property type="protein sequence ID" value="KAI7754294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D7F0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009734">
        <property type="term" value="P:auxin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080162">
        <property type="term" value="P:endoplasmic reticulum to cytosol auxin transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004776">
        <property type="entry name" value="Mem_transp_PIN-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045033">
        <property type="entry name" value="PILS1/3/4/5/7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31651">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31651:SF25">
        <property type="entry name" value="AUXIN EFFLUX CARRIER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03547">
        <property type="entry name" value="Mem_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0927">Auxin signaling pathway</keyword>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="14"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="72"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="106"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="146"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="232"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7754294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="307" mass="33071" checksum="00641B9F850695C4" modified="2024-05-29" version="1">MGFLDLFCAASMPVLKVLIVTALGSFLALDSINILGQSTRKQVNDMVFFVLNPALVSSNLASTITYESMISMWFMPVNILLTFIIGSALGWLLLMIARPPQQLKGLIIGTCAAGNLGNMLLIILPAVCKEKASPFGDPDVCYEYSIAYASLSLAIGAVYLWLYVYNLVRVFAGPPMDSCNKDVASTDEIVTVQDDLTHTLLPSSLSSTNIDGKPKVMLNVMKKQFGNFLRHINLKAVFAPSTIAAIVGFVIGTVGPMRKLLIGTTAPLRVIQDSASLVRDAAIPLVTLIVGGNLLRGSVDHKFTFIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D7Q9</accession>
    <name>A0AAD5D7Q9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">HMA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_023297</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the HIPP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000694">
        <property type="protein sequence ID" value="KAI7755926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D7Q9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009626">
        <property type="term" value="P:plant-type hypersensitive response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044577">
        <property type="entry name" value="HIPP4/7/8/17/18/19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46195">
        <property type="entry name" value="HEAVY METAL-ASSOCIATED ISOPRENYLATED PLANT PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46195:SF2">
        <property type="entry name" value="HEAVY METAL-ASSOCIATED ISOPRENYLATED PLANT PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0488">Methylation</keyword>
    <keyword evidence="4" id="KW-0636">Prenylation</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMA" evidence="7">
        <location>
            <begin position="47"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="7">
        <location>
            <begin position="145"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="108"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="208"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="39"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="121"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="213"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7755926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="332" mass="37442" checksum="27AF38F8EA7B6058" modified="2024-05-29" version="1">MGEQVEKPPEEKKPEEEKKEEAPAKPEEEKKAEESKDAAPPPPPPPPPEIVLRVFMHCEGCARKVRRCLRGFDGVEDIQTDCKTHKVVVKGEKADPLKVLERIQKKSHRQVELLSPIPKPPSEEPKVEEKEAPKPEEKKEEPPQVITVVLKVNMHCEACAQEIRKRILRMKGVETVEPDLKASQVTVKGTFEAPQLVDYVHKRTGKKAIIVKQDPEPKKEDDKAKEEKKDGEEKKEEKKPEGDGGAEKDKEKDKKEEKKEGGGGDEAAKTDAPPAEGGEEIKLMDLRKNEFYYYHPQSYQLAPPRYTAESAYGYPPAPQMFSDENPNACSVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D879</accession>
    <name>A0AAD5D879_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_018915</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010002421">
        <property type="protein sequence ID" value="KAI7754822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D879"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045012">
        <property type="entry name" value="NLP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32002:SF35">
        <property type="entry name" value="PROTEIN NLP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32002">
        <property type="entry name" value="PROTEIN NLP8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7754822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="235" mass="27071" checksum="97BEB72CE41EE746" modified="2024-05-29" version="1">MPNSVEPPGLALISSESRYLTSLHVFAKDLELEADKIDTNHQRIKDLELGVDKINSHDQNVHVKIIAVLKLLTLREQHVLVQFWSPHVVGNQQLLTTIDQPFGLGLMDERLCTYRQDSKRSYFVMNKDKEEEDRSPTTRAFRQGLPEWTPDVANYLPKHFQQQECAIRCNLHGYLALPVFYSNSRLCVGVLELLTSLKHMSYAYEVQQLHNALETVELRTQQAFDCPILNVSKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DA99</accession>
    <name>A0AAD5DA99_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_015513</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000605">
        <property type="protein sequence ID" value="KAI7756074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DA99"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7756074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="114" mass="13282" checksum="FFA07EA49C8F13A9" modified="2024-05-29" version="1" fragment="single">MGHTSTASYYQNEMRLLFVIEMEKRARPCNKWIPTDVDSGCHSLRSFDSDLEEVTTFYVAFLSIHHVQMAIKNKSPYWRGRAATTIQVAWRYRRKRRNSAATSRCGSTYPSNKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DBC2</accession>
    <name>A0AAD5DBC2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_003190</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000116">
        <property type="protein sequence ID" value="KAI7757458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DBC2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35712">
        <property type="entry name" value="MYOSIN HEAVY CHAIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35712:SF1">
        <property type="entry name" value="MYOSIN HEAVY CHAIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="392"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="41"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="158"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="467"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="408"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7757458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="701" mass="79289" checksum="4689315C7572193F" modified="2024-05-29" version="1" fragment="single">KGHRYGTAWWGQTSSEQQTNPSFLLPCHPPACLLSQESVTMDEKFDDRAALLSRIELLERERDELRNDIEHICMQHAEPNYGAVATRMQAQRTAGLEQEIDNLKKRLTACLRENVNLQEELSESYRIKNQLADLHAAELSKSLEAEKQIKFFQSSVAAAFSERDNAIMEAELAKEKEEHASQQFGILQQKIEELNSVVLEEKKRSTTLQTDLEKHEKENELFKQVIAKFYDIRRHSLNEYEDANWEDKCTSLLNDSDDMWTFTGSGETQTTKYITALEEEVEALRKSVANLQSRLQMGLEIEKHLKRMVHDLKNTKMCLEEKMKRDISGLHSFHSQHRLEIVNLLEEESSYFKSVVDEIKENMRQICITEEPNGLSPQREMILQENDCRDVHISPDEASDTTAKENVSELPNTSSSGAGDGSEALVLMLKDKDNISDLQKAISSGTGDISEAFAQALQEKVAALLLLSQQEERYMLENNVNAALQNKLEELQRNLIQVTNEKVVALMELAQLKQENRLLHEKLNDGGKQGKLITASEEKNTFQDKDGRLKGLLKKTYLSQWINPLASHEYVQNITKKSSSSTMDFARMKIEYTALKESLESMEHLISSIRRLRLSLVKVKESVTAEDTVTVASGKSEIIENVVTEAKLLKTALGSTLPVSWSAEVDDGVSNAEKVDSVSAAGFEMVELLILASHLLKNSIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DBL9</accession>
    <name>A0AAD5DBL9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">Pectinesterase</fullName>
            <ecNumber evidence="4 10">3.1.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_025975</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 10">
            <text>[(1-&gt;4)-alpha-D-galacturonosyl methyl ester](n) + n H2O = [(1-&gt;4)-alpha-D-galacturonosyl](n) + n methanol + n H(+)</text>
            <dbReference type="Rhea" id="RHEA:22380"/>
            <dbReference type="Rhea" id="RHEA-COMP:14570"/>
            <dbReference type="Rhea" id="RHEA-COMP:14573"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17790"/>
            <dbReference type="ChEBI" id="CHEBI:140522"/>
            <dbReference type="ChEBI" id="CHEBI:140523"/>
            <dbReference type="EC" id="3.1.1.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the pectinesterase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the PMEI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.11" evidence="4 10"/>
    <dbReference type="EMBL" id="JAMZMK010001697">
        <property type="protein sequence ID" value="KAI7755105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DBL9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004857">
        <property type="term" value="F:enzyme inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030599">
        <property type="term" value="F:pectinesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042545">
        <property type="term" value="P:cell wall modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045490">
        <property type="term" value="P:pectin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd15798">
        <property type="entry name" value="PMEI-like_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.10:FF:000001">
        <property type="entry name" value="Pectinesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.40">
        <property type="entry name" value="Invertase/pectin methylesterase inhibitor family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035513">
        <property type="entry name" value="Invertase/methylesterase_inhib"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033131">
        <property type="entry name" value="Pectinesterase_Asp_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000070">
        <property type="entry name" value="Pectinesterase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006501">
        <property type="entry name" value="Pectinesterase_inhib_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31707">
        <property type="entry name" value="PECTINESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01095">
        <property type="entry name" value="Pectinesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04043">
        <property type="entry name" value="PMEI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00856">
        <property type="entry name" value="PMEI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101148">
        <property type="entry name" value="Plant invertase/pectin methylesterase inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00503">
        <property type="entry name" value="PECTINESTERASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0063">Aspartyl esterase</keyword>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="5 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="25"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="domain" description="Pectinesterase inhibitor" evidence="13">
        <location>
            <begin position="93"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="56"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="66"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7755105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="575" mass="63521" checksum="FCF3C2251E9BB32A" modified="2024-05-29" version="1">MAFQDFDQISERRRLERKQRLKQRIIIGVILTILLLSGAVAAFLIFLNSSRTDGADDAIDNTETTADNKNDKNKDSPSGASKDKGKNDQKILQTQKAIEKICQTTDYKETCLSRLEKSLSSKLSGLPGPVDLMKAAISAASDELDNAINQTSAFMKYETAPENQKAPFDVCKQVFEGARRDFNHSRNAAESPDVGNWLSAALNTTKELTSNSLAIVASLQKKAPPPAANTSRRLLANGAELPTWMSFETRRLLKADGPSLAPNVTVAKDGSGDFTTISKALEELPDSYQGRYVVYIKEGVYEENVVVTKDMVNITMYGDGSQKTIVTGNKNFADGVPTFLTATFAAIGEGFMAQSICFRNTAGPEKLQAIALRVQSDRSIFLNCRMEGYQETLHVQTHRQFYRGCYILGTVDFISGDAAAVFQTCEILIRKPMENQLNTVTAQSRIDRHETTGIVLQKCKISADASLGKSKKIQSYLGRPGKKYSRTIVMESEIDNVIDPAGWLEGEGGFAVETVYYAEYNNIGDGSRMKDRVKWGGYQREFKKEQAENYTVARFIQGESWLKGTGVPVRLGCYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DBS3</accession>
    <name>A0AAD5DBS3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_028788</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000254">
        <property type="protein sequence ID" value="KAI7756812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DBS3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010333">
        <property type="term" value="F:terpene synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.130">
        <property type="entry name" value="Terpene synthase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001906">
        <property type="entry name" value="Terpene_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036965">
        <property type="entry name" value="Terpene_synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050148">
        <property type="entry name" value="Terpene_synthase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005630">
        <property type="entry name" value="Terpene_synthase_metal-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31225">
        <property type="entry name" value="OS04G0344100 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31225:SF137">
        <property type="entry name" value="TERPENE SYNTHASE 11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01397">
        <property type="entry name" value="Terpene_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03936">
        <property type="entry name" value="Terpene_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Terpene synthase N-terminal" evidence="3">
        <location>
            <begin position="8"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="Terpene synthase metal-binding" evidence="4">
        <location>
            <begin position="108"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7756812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="334" mass="39168" checksum="91C0C74DC6C581DD" modified="2024-05-29" version="1">MKEISQRVDVNKRMDELRENTKRALMITSDPKRTLNLIDTIQRLGIGYCFKEEINNILENLTQVLPDDDLYTVALNFRLRRHNGLDTNPAVNHFYILRWWEHLGLSAKWDLNEMEQLPEYMKICFKALYNSNNEICVKVLRERGLSVQPFLRKTWIEMVEAYMVEAEWVKRGRTPTLKDYIKNGVTTSGTYMALVHLFFLVSNGLTTENMQHLLDPYPKFFTVAGTILRLWDDLGTINEEQERGDTLSSIHLLMKEKNITCEEEGRREILQMIYMLWNDLNVELVTPDVMLFPIIKVAVNTSRASQVVYQHNDDSYLSSVEHHVQSLFYKPVDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DD14</accession>
    <name>A0AAD5DD14_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">K-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_032255</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000142">
        <property type="protein sequence ID" value="KAI7757264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DD14"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002487">
        <property type="entry name" value="TF_Kbox"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01486">
        <property type="entry name" value="K-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="K-box" evidence="2">
        <location>
            <begin position="28"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="34"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7757264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="138" mass="15810" checksum="9195C0C7DE336F1C" modified="2024-05-29" version="1" fragment="single">DYDIIVKLHNLNVCQVKSQVRITPAHTKLEGALIQARQRKTQILVEQMEELRRKERELGDINKHLRMKVSHEMSTGYRAQLPCPWNSGVSPGNKLQQHIPNAPISFQSHGLSTRTHLANRVQSVYARGRTISSEEHGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DEW1</accession>
    <name>A0AAD5DEW1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_019480</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000027">
        <property type="protein sequence ID" value="KAI7758115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DEW1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003830">
        <property type="term" value="F:beta-1,4-mannosylglycoprotein 4-beta-N-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006044">
        <property type="term" value="P:N-acetylglucosamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006813">
        <property type="entry name" value="Glyco_trans_17"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12224:SF15">
        <property type="entry name" value="BETA-1,4-MANNOSYL-GLYCOPROTEIN 4-BETA-N-ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12224">
        <property type="entry name" value="BETA-1,4-MANNOSYL-GLYCOPROTEIN BETA-1,4-N-ACETYLGLUCOSAMINYL-TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04724">
        <property type="entry name" value="Glyco_transf_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7758115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="137" mass="15489" checksum="FDD6DF5A94C8CE21" modified="2024-05-29" version="1" fragment="single">MTSALSKFLEMAEGGSRYCSKMSDDVCAHICNEDSGKPSTMARIRCILRGLELKTIIFLLIMVPIIIFGLYVHGQKVSYFLRPLWESPPKPFNEIPHYYHENVSMENLCELHGWGSVSSQGVCLMQFCSLITSFGME</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G2N4</accession>
    <name>A0AAD5G2N4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Carboxypeptidase</fullName>
            <ecNumber evidence="10">3.4.16.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_025463</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the peptidase S10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.-" evidence="10"/>
    <dbReference type="EMBL" id="JAMZMK010011936">
        <property type="protein sequence ID" value="KAI7725458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G2N4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11320:FF:000001">
        <property type="entry name" value="Carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000579">
        <property type="entry name" value="Carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11320">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033124">
        <property type="entry name" value="Ser_caboxypep_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018202">
        <property type="entry name" value="Ser_caboxypep_ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF32">
        <property type="entry name" value="SERINE CARBOXYPEPTIDASE-LIKE 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00560">
        <property type="entry name" value="CARBOXYPEPT_SER_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00131">
        <property type="entry name" value="CARBOXYPEPT_SER_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 10" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041780184" description="Carboxypeptidase" evidence="10">
        <location>
            <begin position="20"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7725458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="468" mass="52313" checksum="B3610B3BE64449EF" modified="2024-05-29" version="1">MLFWTLAFVLLVLFSVNCSITISKPDPIAQQKLDQVSNLPGQSFNVDFEHYAGYVTVNKEYGRALFYWMTEATHDPSSKPLILWLNGGPGCSSIAFGMSEEVGPFHVNEDGKSIYLNPYSWNTVANLLFLDSPAGVGYSYSNTSSDLTSNGDKRTAADSLQFLLNWLERFPQYKGRDFYIAGESYAGHYVPQLSQAIVRYNKANAGSPINLKGYMVGNALTDDYNDHVGLFEFMWAAGMISDQTYKKSNELCDRESFIHSSPECDQITEIASQEMGNIDPYSIFTPPCTANGITKRLLRRWHKVGRIGQTYDPCTEQHSTIYFNLPEVQNALHVHHSNSSRTWESCSGVVRMNWEDSPVSVLDVHQELISAGLRVWIYSGDTDAVIPVTSTRYSINALNLTTISPWRAWYDDGQVGGWTQAYEGLTFVTVRGAGHEVPLHKPKLALTLVKSFLAGTSMTQFIEQVAES</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G562</accession>
    <name>A0AAD5G562_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_019437</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011071">
        <property type="protein sequence ID" value="KAI7729099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G562"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7729099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="68" mass="7694" checksum="7216EE7D773378AB" modified="2024-05-29" version="1" fragment="single">DDDKYEFKEPSKFMEPQTLMIGNFITDIREFSTSVSECNSPAAMIIILVTFLMLTGVCLQNGHNACMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G5T7</accession>
    <name>A0AAD5G5T7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">65-kDa microtubule-associated protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_031299</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MAP65/ASE1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010878">
        <property type="protein sequence ID" value="KAI7729940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G5T7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000911">
        <property type="term" value="P:cytokinesis by cell plate formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007145">
        <property type="entry name" value="MAP65_Ase1_PRC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19321:SF41">
        <property type="entry name" value="FASCETTO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19321">
        <property type="entry name" value="PROTEIN REGULATOR OF CYTOKINESIS 1 PRC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03999">
        <property type="entry name" value="MAP65_ASE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="154"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7729940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="299" mass="33327" checksum="2E61C0760F2A63F2" modified="2024-05-29" version="1" fragment="single">MAELDVQNPLSGETNCGVLLHQLQKIWDDVGESDEERDKMLLQLEQECLDVYKRKVDQASKSRAHLIQALADAKLELSTLCASLGEKTFVGVPDKTSGTIKEQLAAIAPALEQLWKQKEERIKEFLNVQTQIQKIYGEIAGGNQQSGSPKVDESDLSLKKLDEFNDQLQELQKEKSDRLHKVLEYVSTVHDLCGVLGIDFYSTVTEVHPSLNDATGVQSKSISNDTLARLAKTVLALEEDKKQRLKKLQELATHLIDLWNLMDTSKEERSCFDHVTCNISASVGEVTVPGALALDLIEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G6F2</accession>
    <name>A0AAD5G6F2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">UDP-glucuronate decarboxylase</fullName>
            <ecNumber evidence="5">4.1.1.35</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_018516</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the NAD-dependent decarboxylation of UDP-glucuronic acid to UDP-xylose. Necessary for the biosynthesis of the core tetrasaccharide in glycosaminoglycan biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>UDP-alpha-D-glucuronate + H(+) = UDP-alpha-D-xylose + CO2</text>
            <dbReference type="Rhea" id="RHEA:23916"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57632"/>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
            <dbReference type="EC" id="4.1.1.35"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Nucleotide-sugar biosynthesis; UDP-alpha-D-xylose biosynthesis; UDP-alpha-D-xylose from UDP-alpha-D-glucuronate: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the NAD(P)-dependent epimerase/dehydratase family. UDP-glucuronic acid decarboxylase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.35" evidence="5"/>
    <dbReference type="EMBL" id="JAMZMK010011021">
        <property type="protein sequence ID" value="KAI7729296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G6F2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048040">
        <property type="term" value="F:UDP-glucuronate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042732">
        <property type="term" value="P:D-xylose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05230">
        <property type="entry name" value="UGD_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000150">
        <property type="entry name" value="UDP-glucuronic acid decarboxylase 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044516">
        <property type="entry name" value="UXS-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43078:SF49">
        <property type="entry name" value="UDP-GLUCURONIC ACID DECARBOXYLASE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43078">
        <property type="entry name" value="UDP-GLUCURONIC ACID DECARBOXYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16363">
        <property type="entry name" value="GDP_Man_Dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="9" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-binding" evidence="12">
        <location>
            <begin position="83"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00051601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF16363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7729296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="393" mass="44334" checksum="52AFBDC0E53465DF" modified="2024-05-29" version="1" fragment="single">FQTPSNVFELTNRTFLDQTNNSNHTQFQSPNFIDIYSDLSTFCIRLLSMASNASNGDHIATKPPPAPSPLRNSKFLQSNMRILVTGGAGFIGSHLVDKLMENEKNEVIVADNYFTGSKDNLKKWIGHPRFELIRHDVTEPLRIEVDQIYHLACPASPIFYKYNAVKTIKTNVIGTLNMLGLAKRVGARILLTSTSEVYGDPLVHPQPESYWGNVNPIGVRSCYDEGKRVAETLMFDYHRQHGIEIRIARIFNTYGPRMNIDDGRVVSNFIAQALRDEPLTVQAPGTQTRSFCYVSDMVDGLIRLMEGENTGPINIGNPGEFTMIELAETVKELINPKIEIKMVENTPDDPRQRKPDITNAKKMLGWEPKIKLRDGLPLMEADFRQRLGVAKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G7H1</accession>
    <name>A0AAD5G7H1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_011490</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010453">
        <property type="protein sequence ID" value="KAI7731379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G7H1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00018">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.730.10:FF:000001">
        <property type="entry name" value="Ethylene-responsive transcription factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.730.10">
        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001471">
        <property type="entry name" value="AP2/ERF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050913">
        <property type="entry name" value="AP2/ERF_ERF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31194:SF140">
        <property type="entry name" value="ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR CRF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31194">
        <property type="entry name" value="SHN SHINE , DNA BINDING / TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00847">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00367">
        <property type="entry name" value="ETHRSPELEMNT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00380">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51032">
        <property type="entry name" value="AP2_ERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0611">Plant defense</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="8">
        <location>
            <begin position="86"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="159"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7731379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="197" mass="21625" checksum="F807B620D9D14146" modified="2024-05-29" version="1">MNTIFQTLPYLPANPNSIEYVSPSPETLGLLEQDHPAVLEGIAAVVGEHVLFGKSGIMSNKETDYVDVKKETKFGSVSDGGGVEKNYRGVRKRPWGRWSAEIRDKIGRCRHWLGTFDSAEEAARAYDAAARRLRGSKARTNFEIPSVFPLEMSLTTEVKKRKPDGCSSSSSSSSNKCHVVTSVGQLFSTTGPMAKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G883</accession>
    <name>A0AAD5G883_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_001260</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010010553">
        <property type="protein sequence ID" value="KAI7731081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010228">
        <property type="term" value="P:vegetative to reproductive phase transition of meristem"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000309">
        <property type="entry name" value="E3 ubiquitin-protein ligase MBR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045191">
        <property type="entry name" value="MBR1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22937">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF165"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22937:SF170">
        <property type="entry name" value="RING-TYPE E3 UBIQUITIN TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="11">
        <location>
            <begin position="576"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="86"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="151"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="223"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="282"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="356"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="398"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="453"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="106"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="152"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="235"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7731081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="622" mass="66896" checksum="9A2A5AA41CAB8DB4" modified="2024-05-29" version="1">MMQGQKGAIGGSLPESLGFEHGSSSSDSGVVEQQVCWTNFGRNPPVDVNQETQNLSMWSMGEASSSAVPNRVDPKVGHAWSSLMKAQPEVNQREPSAHASRLSLGDGNTNPTHTHANNGHNVSGKKPSNYASSSSGPVEMETRXXXKAAELSIGQSSSGIESSSIYQRPGGSNGVWQPVNENPTPTNTIYFDPIIPRLGSGIGGVSSDNRAIENIRRNVRIRINNSRQQDQLPASNSSNSNNGLLSFSSPHPSLGLNPVAPTGDATSSHAVQPIVQVPAFTRNLHVPSRNRSSNSRATRPWPTNVIGGLGDNSRTTPTNIVDHPLFVQFNDIRNTAQNAVNLDLNSVGGNVNVNGNETSNGNGIASTSNPMPNLRPHRASSLLSSRRHTELLQRYLSSSVGSGAGGGDRGQSNNLFSRIPPPVNAAASSSQETEVPPVVAIAGHHPHRHRARTSDRQLDGGFGFPHLSRAVPGGSERRGRLVSEMRNVLDRLRRGEGLRFEDVMLLDQSVFFGMPDVHDRHRDMRLDIDNMSYEELLALEERIGNVNTGLTEENISKHLKQKTYASDAGQPDAEPCCICQDEYKNGDDLGALGCGHEFHTGCIKQWLLQKNACPVCKSAAAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G8F8</accession>
    <name>A0AAD5G8F8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">dual-specificity kinase</fullName>
            <ecNumber evidence="1">2.7.12.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_010066</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. Lammer subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.12.1" evidence="1"/>
    <dbReference type="EMBL" id="JAMZMK010010260">
        <property type="protein sequence ID" value="KAI7732327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G8F8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004712">
        <property type="term" value="F:protein serine/threonine/tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd14134">
        <property type="entry name" value="PKc_CLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000612">
        <property type="entry name" value="Serine/threonine-protein kinase AFC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051175">
        <property type="entry name" value="CLK_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45646">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE DOA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45646:SF7">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE AFC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="64"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="16"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00051680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7732327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="431" mass="49914" checksum="4667B153A611DF07" modified="2024-05-29" version="1" fragment="single">AQLGLFYGQDVGNATSFAPSRAPQDHNNTSHLKRVAHDGSPPWREDDKDGHYMFAIGDNLTSRYKIHGKMGEGTFGQVLECWDKERKEMVAVKIVRGIKKYREAAMIEIDVMEMERVTEFPMSNVDIGPRKRQRIGWDVVHPAPPKILSRSESLISCVQLRNWFDYRNHICIVFEKLGPSLYDFLRKNNYRSFPIDLVREIGRQLLECIAFMHDLRLIHTDLKPENILLVSPEYIKVPDYKGSSRSPKDSSYSKRIPKCSAIKVIDFGSTTYDRPDQSYIVSTRHYRAPEVILGLGWSYPCDIWSVGCILVELCSGEALFQTHENLEHLAMMERVLGFLPQHMLRKADRHADKYIRKGRLDWPEGATSRESIKAVQKLPRLQNLIMQHVDHSAGDLIHLLQGLLRYDPVDRLTAREALRHPFFTRDHFKRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G942</accession>
    <name>A0AAD5G942_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Amidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_033179</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010357">
        <property type="protein sequence ID" value="KAI7731921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G942"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013105">
        <property type="entry name" value="TPR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46310">
        <property type="entry name" value="AMIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46310:SF4">
        <property type="entry name" value="OUTER ENVELOPE PROTEIN 64, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07719">
        <property type="entry name" value="TPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="domain" description="Amidase" evidence="5">
        <location>
            <begin position="66"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="Amidase" evidence="5">
        <location>
            <begin position="223"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="348"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="382"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7731921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="437" mass="47125" checksum="CFEB4789A5E22148" modified="2024-05-29" version="1" fragment="single">MKSIVSSNPKIWIVIGVTVAGGILIIVASEATRKRRRRSTLREEDDDNYGAFMERFVLIPPPPAAKHPPLSALTFLINDVFDVKDHVTGFGNPDWKTTHDQAPTTAVSVTTLLKNGATCVGKAVMDDFSFGISGENSHYGTPTNPATPSCIPGGSCSGSAVAVAAELVDFAIGTDTVGGVRIPASFCGILGFRPSHGAVSTIGVLPNSQNLDTVAITATHENIEACYMVRTEMRAALHSLLKDDGILAVPTVADTPLKLNSKKTLLSKFNDRAFSLLSIATISGCCQITVPFGKHEDCPLGVSFIANHGSDKFLLDTVLDMYQSLQGQVSAVTSLQPSLDTKGNVEASMLWKEKGNAAYRGKQWQKAITYYTEAVKLDEINATFYCNRAAAYLELGSFQEAEEDCTKAISLDKKNVKAYLRRGTARELVLRYKEALH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G945</accession>
    <name>A0AAD5G945_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_009183</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010387">
        <property type="protein sequence ID" value="KAI7731788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G945"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7731788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="188" mass="21683" checksum="A183BD139046F7F2" modified="2024-05-29" version="1">MELEDMIKESKPIRLISNLTRLYPFKEGSYSTMERVWFYEAQIFTDSQFDFLRYLCLLVNIGPPLKLQFDNSWQNQSFKVWIIKDGEEWSRPSDRKFVSETESNDKSLDIKSAQSVAPDTGVIKDGPRVGMATVQYVGRNLDCLGRMSSRSEVGSDQNGSTGKWVRLDPRVVLNESKVDRVSWRNVAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GAR2</accession>
    <name>A0AAD5GAR2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_009841</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010009690">
        <property type="protein sequence ID" value="KAI7734589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GAR2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001023">
        <property type="entry name" value="Os07g0541700 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000217">
        <property type="entry name" value="probable LRR receptor-like serine/threonine-protein kinase At1g53430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051824">
        <property type="entry name" value="LRR_Rcpt-Like_S/T_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48006">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN DDB_G0281931-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48006:SF68">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="48"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7734589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="207" mass="23027" checksum="7BEECE3B4E9BE02A" modified="2024-05-29" version="1" fragment="single">QLGIFTLQTDFFPHFLIYNIFCLPELRGIDLQTGIFSLRQIKAATKNFDPSNKLGEGGFGSVYKVGLLSDGTLIAVKQLSSKSKQGASEFVNEIGMISALQHPNLVKLYGCCIEGNQMCLIYEYMENNCLSLALFDKVSKAKLTWQVRLNICIGIARGLVYLHEESHMRIIHRDIKASNVLLDENFNAKISDFGLAKLNDDGDTISA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GE25</accession>
    <name>A0AAD5GE25_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Eukaryotic translation initiation factor 6</fullName>
            <shortName evidence="5">eIF-6</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">EIF6</name>
        <name evidence="6" type="ORF">M8C21_002043</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. May also be involved in ribosome biogenesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Monomer. Associates with the 60S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
            <location evidence="5">Nucleolus</location>
        </subcellularLocation>
        <text evidence="5">Shuttles between cytoplasm and nucleus/nucleolus.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the eIF-6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008700">
        <property type="protein sequence ID" value="KAI7738880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GE25"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042256">
        <property type="term" value="P:cytosolic ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00527">
        <property type="entry name" value="IF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.75.10.10:FF:000001">
        <property type="entry name" value="Eukaryotic translation initiation factor 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.75.10.10">
        <property type="entry name" value="L-arginine/glycine Amidinotransferase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00032">
        <property type="entry name" value="eIF_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002769">
        <property type="entry name" value="eIF6"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00323">
        <property type="entry name" value="eIF-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10784">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01912">
        <property type="entry name" value="eIF-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006413">
        <property type="entry name" value="IF-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00654">
        <property type="entry name" value="eIF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55909">
        <property type="entry name" value="Pentein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 5" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3 5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0690">Ribosome biogenesis</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7738880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="237" mass="25704" checksum="F09928A852D48E37" modified="2024-05-29" version="1">MATRLQFENSCEIGVFSKLTNAYCLVAIGGSENFYSTFEAELADVIPVVKTSIGGTRNKNGLLLPHTTTDQELQHLRNSLPDAVVVQRIDERLSALGNCIACNDHVALTHTDLDRETEEMIADVLGVEVFRQTIAGNILVGSYCAFSNRGGMVHPHTSIEDLDELSTLLQVPLVAGTVNRGSEVIAAGLTVNDWTAFCGSDTTATELSVIESVFKLREAQPSAIVDEMRKSLIDTYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GFV6</accession>
    <name>A0AAD5GFV6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_002350</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008800">
        <property type="protein sequence ID" value="KAI7738538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GFV6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7738538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="71" mass="7602" checksum="F2B88A48A484DFC2" modified="2024-05-29" version="1" fragment="single">VQGSAVPNTSRSDGVSLWWCRRPVILEVGSRSGGRVMVRCVWVEFGVLGSDLIDSGSVQVKSALGRSDLLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GI12</accession>
    <name>A0AAD5GI12_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Splicing factor SF3a60 /Prp9 subunit C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_032522</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007743">
        <property type="protein sequence ID" value="KAI7743337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GI12"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051421">
        <property type="entry name" value="RNA_Proc_DNA_Dmg_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024598">
        <property type="entry name" value="SF3a60/Prp9_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12786:SF2">
        <property type="entry name" value="SPLICING FACTOR 3A SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12786">
        <property type="entry name" value="SPLICING FACTOR SF3A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11931">
        <property type="entry name" value="SF3a60_Prp9_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Splicing factor SF3a60 /Prp9 subunit C-terminal" evidence="3">
        <location>
            <begin position="51"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF11931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7743337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="154" mass="18330" checksum="BCABF04AA0A8231A" modified="2024-05-29" version="1" fragment="single">EILSEKIVRTKENVERRQGQTSEEIVAEMEDEEVVDMEVDDEEHQIYNPLNFPMGFDGKPISVWLYKLHGLGQVFECEICGNHSYRGRKAYEQHFNEFRHLYGMHRLGIPNSKSFYEITLIEEARRLWEKIKDTQEANKWNSDLDEEFEDEEGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GJK9</accession>
    <name>A0AAD5GJK9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_026443</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008072">
        <property type="protein sequence ID" value="KAI7742123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GJK9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24126:SF40">
        <property type="entry name" value="ANKYRIN REPEAT FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24126">
        <property type="entry name" value="ANKYRIN REPEAT, PH AND SEC7 DOMAIN CONTAINING PROTEIN SECG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="224"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="419"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="546"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="443"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="486"/>
            <end position="508"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7742123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="617" mass="68043" checksum="1EB78213C12D584D" modified="2024-05-29" version="1">MPPTYFPLRWESTGDQWWYASPIDWAAANGHYDLVRELLRLDSNHLIKLSSLRRIRRLEVVWDDEEQFDDVAKNRSSVVRKLLHEGDAKKGKNSLIGSGYGGWLLYTAASAGDFSFVQELLHKDPLLVFGEGEYGVTDILYAAARGKNTEVFRLIYDLALSPRFMCGNGGELDEHVGEIPPAYKQEMKNRAVHALARGGNLNLLKEVVKYLVSTYEIINSIDKQGNTPLHVAACRGHISVVDFLVKASPSSVHTKNNAGETFLHAAITGFQTPTFRRLDRQIVLMKQLICSKSFNIEEIINTTNNEGRTPLHLAIHGNIDSDLVELIMSVRYINVNKRDNYGMTPLDLLKQGPISSSSEILMRQLVSAGAIFSNQDYHSARKVLANHLRKGSAGGSPGTTFSVSDSEIFLYSGVETSPGTPTLTMNAAGPGPSTSPRLERKSSNKKKHKGIKRFLRWAKMKTTGDSGSVPTTEKKETRVSLRQRYSKTGSPPNNKRTLAARSNNTSPTAKKKLASGLVNGVMQAMPNRRSRSNSFSRSSISSLNSHTGVDIASGSGSNPTCDDGVASYSNKEQGVVHSRKSMNQYFCFGGSKLPVEPPGCDEMQPHEIYDRYVLSTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GJU8</accession>
    <name>A0AAD5GJU8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DC1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_007768</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007693">
        <property type="protein sequence ID" value="KAI7743754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GJU8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004146">
        <property type="entry name" value="DC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47841">
        <property type="entry name" value="DIACYLGLYCEROL KINASE THETA-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47841:SF3">
        <property type="entry name" value="OS09G0492800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03107">
        <property type="entry name" value="C1_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DC1" evidence="5">
        <location>
            <begin position="54"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="DC1" evidence="5">
        <location>
            <begin position="108"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="DC1" evidence="5">
        <location>
            <begin position="168"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7743754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="274" mass="30723" checksum="3471E25CA74893F9" modified="2024-05-29" version="1">MNKRSSSLRIMSQSFQQTNYTHPHPHPLGNFRRSLSIDFPTTSPDDEQMMIHHFTHSHHPIALVNLQDPFTCSGCKERGTGKRFVCQMCDFQLHDFCALSPPFLKAHPLHAHHQLVFFSKPKTGGIRWPTCNVCGKATRGFMFRCNICHFQMHPCCAMLSDQINYPSLHRHPLNLLPPDDQPCGVCNRKRSGRLYGCRTCGYYLHAVCAKDLINGRQVSSEKPSVLGPAVRFASQAVIDFIGGLIDGIGEGVGEALVQNVTRSPAGRRNGTRRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GKZ1</accession>
    <name>A0AAD5GKZ1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">L-ascorbate oxidase homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_007707</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the multicopper oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006894">
        <property type="protein sequence ID" value="KAI7746800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GKZ1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13846">
        <property type="entry name" value="CuRO_1_AAO_like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13894">
        <property type="entry name" value="CuRO_3_AAO_like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000012">
        <property type="entry name" value="Monocopper oxidase-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000016">
        <property type="entry name" value="Monocopper oxidase-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001117">
        <property type="entry name" value="Cu-oxidase_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034273">
        <property type="entry name" value="CuRO_1_AAO-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034275">
        <property type="entry name" value="CuRO_3_AO-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF11">
        <property type="entry name" value="L-ASCORBATE OXIDASE HOMOLOG ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00394">
        <property type="entry name" value="Cu-oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042050871" description="L-ascorbate oxidase homolog" evidence="3">
        <location>
            <begin position="23"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="6">
        <location>
            <begin position="33"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="4">
        <location>
            <begin position="159"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="5">
        <location>
            <begin position="376"/>
            <end position="513"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7746800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="541" mass="60961" checksum="C4786A5BE774CE6F" modified="2024-05-29" version="1">MVEKRNLVFICSLFLFITIINGEDPYRFFTWKVTYGDIYPMGVKQQGILINGQFPGPQINCVTNDNLIISVYNYLNEPFLLSWNGLQHRRNSWQDGTYGTTCPIPPGKNFTYMLQAKDQIGSFFYFPSLGLQKAAGGYGGIKIYSRPRIPVPFPFPAGDHTVLAGDWYKRGHQRLRHILDMGRSISSPDGLVINGRGWNGYTFEVEQGKTYRFRISNVGLTTSINFRIQGHTMKLVEVEGSHTLQNTYESLDLHLGQSASVLVTANKPARDYYVVVSSRFTRRVLTTTAVLHYKNSKTGVSGPPPGGPTTQIAWSLMQARSIRWNLTASGPRPNPQGSYHYGMIKPGHTIVLSNSAPWINGKQRYAVNGVSFVAGDTPLKLADYFNIGGVFNVGSIPDKPSNGNAYLATSVMHADFRSFAEIVFQNWEDTVQSWHIDGYSFFVVGMDGGQWTSASRSRYNLRDTVARSTVQVYPKAWTAIYIALDNVGMWNIRSENWVRQYLGQQFYLRVYSPAHSWRDELPIPKNALLCGRARGRHTRPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GL70</accession>
    <name>A0AAD5GL70_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">leucine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Leucyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">M8C21_027007</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">AA19_3_7</strain>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>tRNA(Leu) + L-leucine + ATP = L-leucyl-tRNA(Leu) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:11688"/>
            <dbReference type="Rhea" id="RHEA-COMP:9613"/>
            <dbReference type="Rhea" id="RHEA-COMP:9622"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78494"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.4" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010006855">
        <property type="protein sequence ID" value="KAI7747042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GL70"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004823">
        <property type="term" value="F:leucine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006429">
        <property type="term" value="P:leucyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009791">
        <property type="term" value="P:post-embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048608">
        <property type="term" value="P:reproductive structure development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07959">
        <property type="entry name" value="Anticodon_Ia_Leu_AEc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.730.10:FF:000020">
        <property type="entry name" value="Leucine--tRNA ligase cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.740.10:FF:000001">
        <property type="entry name" value="Leucine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.740.10">
        <property type="entry name" value="Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004493">
        <property type="entry name" value="Leu-tRNA-synth_Ia_arc/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055416">
        <property type="entry name" value="RBD_LARS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00395">
        <property type="entry name" value="leuS_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45794:SF1">
        <property type="entry name" value="LEUCINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45794">
        <property type="entry name" value="LEUCYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24810">
        <property type="entry name" value="RBD_LARS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="ValRS/IleRS/LeuRS editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="12">
        <location>
            <begin position="36"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="12">
        <location>
            <begin position="206"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="domain" description="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase anticodon-binding" evidence="13">
        <location>
            <begin position="806"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine--tRNA ligase RagD-binding" evidence="14">
        <location>
            <begin position="955"/>
            <end position="1032"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="133"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="143"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF24810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAI7747042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="1100" mass="125292" checksum="70EFF1C047CFC9F2" modified="2024-05-29" version="1">MSFNSFCQISNEMADTDTGRSFKRRDQLLEMEHEVRKWWDESHVFNAEALDQPAKEGDKFFGTFPFPYMNGYLHLGHAFSLSKLEFAAAYHRLRGANVLLPFGFHCTGMPIKASADKLRREIEKFGNPPVFPSVETEEVVNEPEPKPDEGSNPEKFKGKKSKAAAKTGRETYQWEIMRGYGLSDAEIATFQDPVQWLRFFPPLAVEDLKAFGLGADWRRSFITTDINPFFDKFVRWQMRKLKSFGKIVKDLRYTIYSPLDGQPCADHDRASGEGVIPQEYTLIKMEVVPPFPPKFAHLEGKKVYLAAATLRPETMYGQTNSWVLPDGKYGAFEINESEVFILTERAARNLAYQRLSRVPEKATCLVELTGQDLIGLPLRSPLSFNEIIYCLPMLSVLTDKGTGIVTSVPSDSPDDFMALQDLKAKAPFRAKFGVKDEWVLPFEVIPIISHPEFGDKSAEKICTDLKIKSQNEREKLDAAKKVIYKGGFYEGTMIAGEYAGMRVQDAKNLIRSKLLELNQAVVYSEPEKKVMSRSGDECVVALTDQWYLTYGEPSWRETAEECLANMNLYSDETRHGFEHTLSWLNQWACSRSFGLGTRIPWDEEFLVESLSDSTIYMAYYTVCHLLQKGDLFGVDTSTVKPEQLTDDVWDYLFLSGPEPKSSDISLTLLKKMRQEFQYWYPVDLRVSGKDLIQNHLTFCIYNHTAIFPKEHWPRGFRCNGHIMLNSEKMSKSTGNFRTLKQAIEEFSADATRFSLADAGDGMDDANFVFETANAAILRLTKERAWMEEVLAAESALRAGPPSIYADRVFANEMNFAIKMTQKNYSDYMFREALKSGFYDLQAARDEYRFSCGSSGMNRDLVWRFMDIQTRLIAPICPHYSEYVWKKLLKKEGFVIKAGWPEAENPDLTLQKANRYLQESITNFRKLLLKQASGGSKKGSNKASSQSKPTIGLVFVNEQYDGWKRECLNILRDKFDNQNRKFAPDQEILQALQQSAVAQEGNFKQIQKLCMPFLRFKKDEVMTLGTQALDLRLPFGEIEVLQENLELIKRQIGLDHVEILSATDSEAVNKAGRHASLLKQTPPSPGSPTSIFLTKDGMLIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GLJ5</accession>
    <name>A0AAD5GLJ5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_029423</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007457">
        <property type="protein sequence ID" value="KAI7744716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GLJ5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000041">
        <property type="entry name" value="TBC domain containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000028">
        <property type="entry name" value="TBC domain containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.750:FF:000007">
        <property type="entry name" value="TBC1 domain family member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.750">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957:SF26">
        <property type="entry name" value="LD44506P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="2">
        <location>
            <begin position="182"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="19"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7744716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="469" mass="53989" checksum="B748C32E7CBEC032" modified="2024-05-29" version="1" fragment="single">KKEVLYNTNHHGHHHQTMKNNNSNNSHSKQQQDLKQQETSPTNSHITSLDSRFNQTLRNVQGLLKGRSFPGKVLITRRSDPPDGAILGSPVASRSPSEIDSPPDKHMDDPNEDEVHINKPNASTSGNKLKPSMSNPEDRSKEAPRFVMGARATDTARLMKFTKVLGGTTVILDKLRELAWSGVPPYLRPNVWRLLLGYAPPNSDRREGVLRRKRIEYFDCVAQYYDISDNERTDEEITMLRQIAVDCPRTVPDVTFFQQAAVQKSLERILYTWAIRHPASGYVQGINDLATPFLVVFLSEHLEGNPAKISDVEADCYWCLSKLLDGMQDHYTFAQPGIQRLVFKLKELVRRIDEPVAKHIDEQGLEFLQFAFRWFNCLLIREIPFHLVTRLWDTYLAEGDALPDFLVYIFASFLLTWSDKLLKLDFQEMVMFLQHLPTNNWSHQELEMVLSRAFMWHTMFDSCPNHLAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GLW2</accession>
    <name>A0AAD5GLW2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_013237</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006670">
        <property type="protein sequence ID" value="KAI7747822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GLW2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031072">
        <property type="term" value="F:heat shock protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10747">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10719">
        <property type="entry name" value="DnaJ_zf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.260.20:FF:000005">
        <property type="entry name" value="Chaperone protein dnaJ 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.230.10:FF:000002">
        <property type="entry name" value="Molecular chaperone DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.230.10">
        <property type="entry name" value="Heat shock protein DnaJ, cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.260.20">
        <property type="entry name" value="Urease metallochaperone UreE, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01152">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012724">
        <property type="entry name" value="DnaJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002939">
        <property type="entry name" value="DnaJ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008971">
        <property type="entry name" value="HSP40/DnaJ_pept-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001305">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036410">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008035">
        <property type="entry name" value="PRK10767.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43096">
        <property type="entry name" value="DNAJ HOMOLOG 1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43096:SF52">
        <property type="entry name" value="DNAJ HOMOLOG 1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01556">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00684">
        <property type="entry name" value="DnaJ_CXXCXGXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57938">
        <property type="entry name" value="DnaJ/Hsp40 cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49493">
        <property type="entry name" value="HSP40/DnaJ peptide-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51188">
        <property type="entry name" value="ZF_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="1 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="J" evidence="7">
        <location>
            <begin position="73"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="CR-type" evidence="8">
        <location>
            <begin position="195"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="CR-type" evidence="6">
        <location>
            <begin position="195"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7747822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="426" mass="46938" checksum="0187A28E2B7D1031" modified="2024-05-29" version="1">MVRSNAIKLFSWLARRSLSNDVLYDSLVRGGYRKFGSSIHGHNHYRNVGNWRYGSHIGRVNACYGNTFMAAKDFYDTLGVPKNATASEVKKAYYGLAKKWHPDANKDDPEAERRFQEISKAYEVLKDEEKRAQYDQLGHETFEASANGAGPDPRHWRNPFQDLSDIFGFGPFGGQFTGKDAKVALELSFMEAVQGCTKNIVFQTELPCETCGGTGVPPGSKPETCRRCKGAGMIFTQHGPLRMQAICTQCGGSGRYFKSLCKSCNGQRVVRGPKSVKVNIMPGVDTDEELRIPRSGGADPDGNQPGDLFVVIKVREDPVFRREGSNIHVDAVLNITQAILGGTIQVPTLTGDVVLKVRPGTQPGQKVVLKGKGIKSRNSYSYGDQYVHFNVSMPKNLTERQRELIEEFAKEEQGEDEKGAAAGASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GP31</accession>
    <name>A0AAD5GP31_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_023458</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006226">
        <property type="protein sequence ID" value="KAI7750057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GP31"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7750057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="71" mass="8146" checksum="0028797C19D9A13A" modified="2024-05-29" version="1" fragment="single">CNNNERYQELINTRPSIPARRPISQISYQIEASVKNIHTTSSPDQRRRPYCGLFAATTTSVCEYKKITSIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GP87</accession>
    <name>A0AAD5GP87_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aminotransferase class I/classII large domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_019094</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006223">
        <property type="protein sequence ID" value="KAI7750070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GP87"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004838">
        <property type="term" value="F:L-tyrosine-2-oxoglutarate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006572">
        <property type="term" value="P:L-tyrosine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45744">
        <property type="entry name" value="TYROSINE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45744:SF11">
        <property type="entry name" value="TYROSINE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="1">
        <location>
            <begin position="48"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7750070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="278" mass="30937" checksum="DD282B4BBB0B3B5A" modified="2024-05-29" version="1">MGIENGSTLKKKWGFLKNKETNMVPDITIRGVRYMLMSKLNKSDTRPVLPLAHGDPSAFACFRTTQIAEDALVNSIRSGNFNGYGPGVGIPQARRAVADYLSQDLPNKLSEEDVFLTVGCAHAIQTIITVVANPNANILLPKPGFPYYEAVAKSCHLEVRHFELLPEKDWEVDLDAVEALADENTLAMVIINPGNPCGNIFTRKHLEKVKLDLFAFEDIKDDLDFCLKLAKEESVIILPGITVGLKNWLRITYAIEPSALEDGIKRLKAFCERHSNKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GPM6</accession>
    <name>A0AAD5GPM6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CCR4-Not complex component Not1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_012429</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006496">
        <property type="protein sequence ID" value="KAI7748629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GPM6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030015">
        <property type="term" value="C:CCR4-NOT core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000288">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.800:FF:000001">
        <property type="entry name" value="CCR4-NOT transcription complex subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.800">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007196">
        <property type="entry name" value="CCR4-Not_Not1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040398">
        <property type="entry name" value="Not1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13162">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13162:SF8">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04054">
        <property type="entry name" value="Not1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCR4-Not complex component Not1 C-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7748629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="342" mass="39009" checksum="C767F2000DDBF081" modified="2024-05-29" version="1">MPKLLSGNGQKGWPYFQRLLVDLFQFMEPFLRNAELAEPVRFLYKGTLRVLLVLLHDFPEFLCDYHFSFCDIIPPSCIQMRNIVLSAFPRNMRLPDPSTPNLKIDLLAEINQSPRILSEVDAALKAKQMKSDVDEYLKTMPQGTSFLSELKKKLLLSPSEAARAGTRYNVPLMNSLVLYVGIQAIQQLQARTPHGQSMASNASQAILMVGDIFQTLILELETEGRYLFLNAIANQLRYPNNHTHYFSFILLYLFSESNQEAIQEQITRVLLERLIVNRPHPWGLLITFIELIKNPRYNFWSRSFTRCAPEIEKLFESVSRSCGGPKPVDDGVVVGGISDAMH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GQN7</accession>
    <name>A0AAD5GQN7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_024742</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the PP2C family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010005817">
        <property type="protein sequence ID" value="KAI7751797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GQN7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000291">
        <property type="entry name" value="Protein phosphatase 2C 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000222">
        <property type="entry name" value="PP2C_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01032">
        <property type="entry name" value="PPM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0464">Manganese</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="11">
        <location>
            <begin position="71"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="303"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="11"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7751797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="358" mass="39329" checksum="F6368198C1E6B182" modified="2024-05-29" version="1">MADKSFGVGETSSPPTSGGSTQGGRRPIAYAASNMLIPLPESNPMRRKFDSSDSTTEFSGESVVLDKERCRFGLKVVCGRRRDLEDTVAVKPSFCKVNSPSCDDLHFYGVYDGHGCSHVAMKCKERMHEIVKEEVENYEEVIEWKELMVKCFSRMDKDITEWSNNATSSDCRCELQTPQCDAVGSTTVVAVVAPDKIVVSNCGDSRAVLCRNGPDRPDELERIEKAGGRVLFWHGARVLGVLAMSRAIGDNYLKPYVIPEPEVTVTERTDDEECLIIASDGLWDVVSNDVACRVARMCLRSGEVPSPPRSEGSPFNVAQGESSDKACSDASIMLTKLALVRRSSDNVSVLVVDLRRDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GQQ5</accession>
    <name>A0AAD5GQQ5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Succinate dehydrogenase subunit 5, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_009306</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006523">
        <property type="protein sequence ID" value="KAI7748483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GQQ5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045273">
        <property type="term" value="C:respiratory chain complex II (succinate dehydrogenase)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025397">
        <property type="entry name" value="SDH5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36139">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE SUBUNIT 5, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36139:SF1">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE SUBUNIT 5, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14290">
        <property type="entry name" value="SDH5_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7748483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="266" mass="29786" checksum="5B49684CA7167BA2" modified="2024-05-29" version="1" fragment="single">VTYIGKTKLNISVLIILQQQTHSFNKLLLRSFIFHSVRTVMEKLMMMRSIYRSISRRSLPFIASSAAVATNHQCLRRGFHHSPPSMLSNNRSPFALGIGSTRCFSDGLTHLPDIKDGDIKLAFKELMAVNWDELPDAVVNKTKRALSKNTEDKASQEALANLLRAAEAVEEFSGFLVTLKMEIDDSVGMSGENVKPLTNEVANALSVAFGRYYAYLDSFGPDEGYLRKKVENELGMKLIHLKMRCSGLGGDWGKWQTEALQQKPED</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GRA0</accession>
    <name>A0AAD5GRA0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Prostaglandin E synthase 2</fullName>
            <ecNumber evidence="3">5.3.99.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Microsomal prostaglandin E synthase 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">M8C21_032963</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">AA19_3_7</strain>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>prostaglandin H2 = (12S)-hydroxy-(5Z,8E,10E)-heptadecatrienoate + malonaldehyde</text>
            <dbReference type="Rhea" id="RHEA:48644"/>
            <dbReference type="ChEBI" id="CHEBI:57405"/>
            <dbReference type="ChEBI" id="CHEBI:90694"/>
            <dbReference type="ChEBI" id="CHEBI:566274"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:48645"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>prostaglandin H2 = prostaglandin E2</text>
            <dbReference type="Rhea" id="RHEA:12893"/>
            <dbReference type="ChEBI" id="CHEBI:57405"/>
            <dbReference type="ChEBI" id="CHEBI:606564"/>
            <dbReference type="EC" id="5.3.99.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:12894"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism; prostaglandin biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="18">Endomembrane system</location>
            <topology evidence="18">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GST superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.99.3" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010005721">
        <property type="protein sequence ID" value="KAI7752215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GRA0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050220">
        <property type="term" value="F:prostaglandin-E synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03197">
        <property type="entry name" value="GST_C_mPGES2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011767">
        <property type="entry name" value="GLR_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034334">
        <property type="entry name" value="PGES2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034335">
        <property type="entry name" value="PGES2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12782">
        <property type="entry name" value="MICROSOMAL PROSTAGLANDIN E SYNTHASE-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12782:SF11">
        <property type="entry name" value="PROSTAGLANDIN E SYNTHASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13417">
        <property type="entry name" value="GST_N_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01182">
        <property type="entry name" value="Prostaglandin_E_synthase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01203">
        <property type="entry name" value="Prostaglandin_E_synthase_like1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00195">
        <property type="entry name" value="GLUTAREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="14" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0643">Prostaglandin biosynthesis</keyword>
    <keyword evidence="5" id="KW-0644">Prostaglandin metabolism</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="GST N-terminal" evidence="19">
        <location>
            <begin position="95"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00031041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00037847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAI7752215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="326" mass="37615" checksum="A964E51D7211CA4E" modified="2024-05-29" version="1">MATTMRNLNRLTTTIRTASSLLIHQTASYSGITRSSNTFSYLPQSHWLLLSNISRELTHGRNCHGCNISFSTDVSAARDFDVKDVEKSNKSYVPNDVVLYQFEACPFCNKVQAFLEYYDIPYKVVEVNPMNKKEIKWSDYKKVPILMVDGEQMINSSDIIDKMYQRMHPDSVTEDAETQEWRRWVDDHLVHVLSPNIYRSPSEALESFDYITTKGNFSFTERLVAKYGGAAAMYFVSKTLKKRHNITDERRALYEAADKWVRALNGRHYLGGSEPNLADLSVFGVLRPIRYLKSGKDMVENTRIGDWFTRMENQMKPHRDTTLQGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GSB3</accession>
    <name>A0AAD5GSB3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">BI1-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_015962</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the BI1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006175">
        <property type="protein sequence ID" value="KAI7750253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GSB3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006214">
        <property type="entry name" value="Bax_inhibitor_1-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23291:SF95">
        <property type="entry name" value="BAX INHIBITOR 1-RELATED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23291">
        <property type="entry name" value="BAX INHIBITOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01027">
        <property type="entry name" value="Bax1-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="52"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7750253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="78" mass="9111" checksum="93BBA49907B918CE" modified="2024-05-29" version="1">MVLIVFSFIQIFFPMGKISVMIYAGLSAIIFCGYIVYDTDNLIKRYTYDEYIWAAVALYLDIINLFLSLLNILRVLDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GWD5</accession>
    <name>A0AAD5GWD5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">phenylalanine--tRNA ligase</fullName>
            <ecNumber evidence="5">6.1.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Phenylalanyl-tRNA synthetase alpha subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">M8C21_014100</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">AA19_3_7</strain>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>tRNA(Phe) + L-phenylalanine + ATP = L-phenylalanyl-tRNA(Phe) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19413"/>
            <dbReference type="Rhea" id="RHEA-COMP:9668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78531"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha subunit type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.20" evidence="5"/>
    <dbReference type="EMBL" id="JAMZMK010001017">
        <property type="protein sequence ID" value="KAI7755559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GWD5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009328">
        <property type="term" value="C:phenylalanine-tRNA ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004826">
        <property type="term" value="F:phenylalanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006432">
        <property type="term" value="P:phenylalanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00496">
        <property type="entry name" value="PheRS_alpha_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000033">
        <property type="entry name" value="Phenylalanine--tRNA ligase alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004529">
        <property type="entry name" value="Phe-tRNA-synth_IIc_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002319">
        <property type="entry name" value="Phenylalanyl-tRNA_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040725">
        <property type="entry name" value="PheRS_DBD3"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00468">
        <property type="entry name" value="pheS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11538:SF40">
        <property type="entry name" value="PHENYLALANINE--TRNA LIGASE ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11538">
        <property type="entry name" value="PHENYLALANYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18553">
        <property type="entry name" value="PheRS_DBD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01409">
        <property type="entry name" value="tRNA-synt_2d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0436">Ligase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="16">
        <location>
            <begin position="255"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00030612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAI7755559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="403" mass="46175" checksum="4416E5F1B21CCE46" modified="2024-05-29" version="1">MAEEAILGFLEHNEEIVDSGEFAVEKDISHEDIVKVVKSLHGYGLVVAQKKLGDSIFKIGCQQAMKNKWVEMGKTQVSRKVARVDDNVKALLIKINDGEALIQEDIDALKRRKLISPQIWKGYSVKKGPKYAPKRIKPATDLTRENLQRFEEMPTNNYVESSFWNFDALFQPQQHPARDSHDTFFLREPSTTKTLPEDYVQRVKQIHESGGHGSRGYGYDWKREEANKNLLRTHTTAVSSRMLYALAKKPFEAKRYYSIDRVFRNEAVDRTHLAEFHQIEGLICDRGLSLGDLQGVLHDFFSRLGMSNLRFKPAYNPYTEPSMEIFGYHEGLKKWVEVGNSGMFRPEMLLPMGLPEDVTVIAWGLSLERPTMILYGIDNIRDLFGHKVDLSLIKSNPICRIGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GWN4</accession>
    <name>A0AAD5GWN4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_022769</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010000015">
        <property type="protein sequence ID" value="KAI7758245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16664">
        <property type="entry name" value="RING-Ubox_PUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055566">
        <property type="entry name" value="ARM_LIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056514">
        <property type="entry name" value="ARM_LIN_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052858">
        <property type="entry name" value="E3_ubiquitin-ligase_LIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056512">
        <property type="entry name" value="LIN_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045210">
        <property type="entry name" value="RING-Ubox_PUB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003613">
        <property type="entry name" value="Ubox_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47446">
        <property type="entry name" value="RING-TYPE E3 UBIQUITIN TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47446:SF2">
        <property type="entry name" value="RING-TYPE E3 UBIQUITIN TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23568">
        <property type="entry name" value="ARM_LIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23654">
        <property type="entry name" value="ARM_LIN_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23628">
        <property type="entry name" value="ARM_LIN_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04564">
        <property type="entry name" value="U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00504">
        <property type="entry name" value="Ubox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51698">
        <property type="entry name" value="U_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0853">WD repeat</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="domain" description="U-box" evidence="9">
        <location>
            <begin position="450"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="1140"/>
            <end position="1174"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="1341"/>
            <end position="1368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="362"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="230"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7758245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="1368" mass="152362" checksum="AC0D29454BED5F09" modified="2024-05-29" version="1" fragment="single">SKAAVLYINNPANTIIIRFIIIIIRLILCNNFSKLTPPSTTMSTTTTTTILHHTTTFLHHTLSQPDHRHRVFSILLTTLSPSLHPIINHLTLSQQTLENALTTINPSIKSSSLHLSQKLLLSDPNNAVSTFLLSLIYTLCDKHVDACITFLELFETHPSIARIEIAPQLFQDLFLIYFVPVLEWYNEKRSRIVSSLNSNNNNMPNIVNNDGYGYESDENSVVMETGVSCKHLLSKMNDDQAMELKELEREYEEVLDENCRVLGNKDGERVIDPPSVVLEAVYKGEKCEYGQDGKSASAEFGSENGRLMLISIFLLEVAKWSGQMPIWTDKGEKSIGIQIKKTKDLSRFPSFFPERVSPRVLTDERSSLRSIKPSPDNNDSGSEPEDNFRSMSSFESDAELEEKNVEITSLDTKKTLSQKQKQKQPVILESSSFDPLMGDYDNLINEGKHTPPKDFVCPITTHIFSDPVTLETGQTYERKAIQQWLERGNSTCPITRQKLHSTQLPKTNYILKHLIASWKELNPSNSNQENEQLDLDIPSLTLHSPDDGTVNELRVAITKLCTSEVLKEAETAVFTIERFWQDVTESGVGPEVEIQDMLSQPPVINGFMEILFSSVNTRVLISTVFLLSELGSRNEDVISTLTRVDSDVECVIALFQKGLFEAVVLIYLLKPSVPTLVQVELVDSLLSVVSKNESEFFKMCVKPKTAAVLLLGQIVSCGDDVAVFEVVRRVMSGKVIESIISSLESEWTEERITAVRLLLKCIQEDGKCRNIIADKAELAPVLEILVGANDGDRFEIVQLLSELVKLNRRTFNNQILHIIKDEGTFSTMHTLLAYLQTAEAEQSPVVAGLLLQLDLLAEPRKMSIYREEAIDTLISCLKNSDSPSAQVAAAETILALQGRFSSFGKPLIRAYLLKHVGLDKSYRSTMRKEQLAAISGDIQESMEEEKAAEEWERKTAFVLVSHEFGLIFEGLSEGLKSNYAEICSACFVCATWLVHMLTLLPDTGIKGAARICLLNRFVSIFKSAKDTEDKALSMLALKSFIHDPDGQREISPHMKDILKGLREFKKSSKVAFEMLKVLSEGSESSADLWNHKELAQQDCSVNGEVSSIVCFKDKIFTGHSDGMIKVWTGRGSVLHLLQEIREHSKGVTSLTVLQSGDTLYSGSHDKTVRGWSVNKEMIQCEQVYDVKDHVSNLLVANSISCFIPQGAGIKIHSWSGTSKLLNPSKHVKCLALEIDLATGTLNSIQSGSRKLLGKSTPVHALKVHDGLIYSASSSIEGTALKVWNASNYTLTHSVSLTSEVRAMVISSDMIYMGCKGGIVEVWCKKKLTRKEVLQIGTNCKVTCMDLDNNEDYLVVGTSDGRIQAWGVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GY57</accession>
    <name>A0AAD5GY57_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="3">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_019180</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010000140">
        <property type="protein sequence ID" value="KAI7757269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GY57"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000708">
        <property type="entry name" value="Peptidylprolyl isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039663">
        <property type="entry name" value="AIP/AIPL1/TTC9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11242">
        <property type="entry name" value="ARYL HYDROCARBON RECEPTOR INTERACTING PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11242:SF0">
        <property type="entry name" value="TPR_REGION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0697">Rotamase</keyword>
    <keyword evidence="2 4" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="361"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase FKBP-type" evidence="7">
        <location>
            <begin position="90"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="4">
        <location>
            <begin position="287"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="50"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7757269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="388" mass="44059" checksum="DDA149CA586CA1B1" modified="2024-05-29" version="1">MDEVQQIGSVGQDGETEIMPNGSSVTHGEPPQNGQDAEEEIVMEGSSFVQGGPPQDDNGPPKVDTKVETIHEKVTKQVIKEGHGEKPSRYSTCFLHYRAWTESTQHKFEDTWQELQLTEVVLGKEKKEMAGLALGLYSMKAGERALLHVGWELGYGEEGSFSFPNVPPKADIIYEVELVGFDETKEGKARSEMTVEERISAADRRRMDGNNLFKEDKLEEAMQQYEMAIAYMGDDFMFQLFGKYQDMAVAVKNPCHLNMAACLIKLKRYEEAIAQCSIVLAEDQNNVKALFRRGKARSELGQTDAAKEDFTKARKLAPEDKAISKELRLLAEHEKIVYQKQKELYKGLFGPRPEPKKPKTITSWFVVVCQWLISLFYGLFRRERIKTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GYF6</accession>
    <name>A0AAD5GYF6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA-directed RNA polymerase</fullName>
            <ecNumber evidence="2">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_008187</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNA polymerase beta chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010000106">
        <property type="protein sequence ID" value="KAI7757524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GYF6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032549">
        <property type="term" value="F:ribonucleoside binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.270.10">
        <property type="entry name" value="DNA-directed RNA polymerase, subunit 2, domain 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015712">
        <property type="entry name" value="DNA-dir_RNA_pol_su2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007120">
        <property type="entry name" value="DNA-dir_RNAP_su2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037033">
        <property type="entry name" value="DNA-dir_RNAP_su2_hyb_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20856">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00562">
        <property type="entry name" value="RNA_pol_Rpb2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA-directed RNA polymerase subunit 2 hybrid-binding" evidence="7">
        <location>
            <begin position="23"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7757524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="105" mass="12216" checksum="5C69A3AB8C0005E7" modified="2024-05-29" version="1">MVSELYEASKQTANPWIFEPKFLGKSRIFNRRTRDPFEQPFIIGKPYILKLINQVDDKIHGCSSGRYSRLTQQPLKEVLGTITFGERIPTPEDAPESFQLFIREL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GYT2</accession>
    <name>A0AAD5GYT2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Agenet domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_018680</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000036">
        <property type="protein sequence ID" value="KAI7757959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GYT2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd20405">
        <property type="entry name" value="Tudor_Agenet_AtDUF_rpt1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008395">
        <property type="entry name" value="Agenet-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014002">
        <property type="entry name" value="Agenet_dom_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31917">
        <property type="entry name" value="AGENET DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31917:SF114">
        <property type="entry name" value="AGENET-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05641">
        <property type="entry name" value="Agenet"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00743">
        <property type="entry name" value="Agenet"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Agenet" evidence="2">
        <location>
            <begin position="47"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="Agenet" evidence="2">
        <location>
            <begin position="149"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="237"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="312"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7757959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="322" mass="35371" checksum="9F6DE9280C974A3E" modified="2024-05-29" version="1">MSQCQKYIPTYQIKHKTSTHSSDPSNKPLNPHFQSCEAIYTTMSNTTQITKGSSVEVCSNDPGFEGAWYVGTLIDQLNPNPNPNQPKYIIKYDTLLKDDGSNDPLTETVDPSFVRPLPPRNLRRNNIKTAAVVVAAADVEGKNDGGGGGEFEMYDVVDAYHREGWWIGVVKKVVVNNNIGGGRRYVVGFENPPEEVEFGRGQLRLHVDWVDGRWRVPPKKIPKDVLLARKAKLVSESNNDARSDLATPSKGAQTTDADSPTVDPLLSSLKKSLSKKNTEGNAEMGAVEASDPSKKNKRVTTSVEQDSRGKRFQSSAGVENIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BK42</accession>
    <name>A0AAD5BK42_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_021479</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012172">
        <property type="protein sequence ID" value="KAI7724668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BK42"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004525">
        <property type="term" value="F:ribonuclease III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030422">
        <property type="term" value="P:siRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00593">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1520.10">
        <property type="entry name" value="Ribonuclease III domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014720">
        <property type="entry name" value="dsRBD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000999">
        <property type="entry name" value="RNase_III_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036389">
        <property type="entry name" value="RNase_III_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14950">
        <property type="entry name" value="DICER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14950:SF49">
        <property type="entry name" value="RIBONUCLEASE 3-LIKE PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00035">
        <property type="entry name" value="dsrm"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14622">
        <property type="entry name" value="Ribonucleas_3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00358">
        <property type="entry name" value="DSRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00535">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69065">
        <property type="entry name" value="RNase III domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50137">
        <property type="entry name" value="DS_RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50142">
        <property type="entry name" value="RNASE_3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RNase III" evidence="5">
        <location>
            <begin position="5"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM" evidence="4">
        <location>
            <begin position="145"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM" evidence="4">
        <location>
            <begin position="230"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7724668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="307" mass="34302" checksum="1E225950479A0BA7" modified="2024-05-29" version="1">MEDSIKAVEALLMYRFKNKKLLEEALTHSSCTDGVSYQRLEFLGDNVIALAISNFFYLNYPEVDAGVLSRLLAANVFEFLIEVDEEDMVVYGGQMKAPKVLADIVESVAAALYIDLGFNLQDMWMIFRHLLEPLVMLDVIMAEPQPITALYEACQKNGKQVDIQYRRNGDRTMASVFVDGAFVASRSSDTKENAKLHAAEAALAKITKFKSCDTGPQAIVDFNEAMEIDGAKHKLNELCNKKRWPKPTYKLEQESGPSHDRRYVSSVQLELPDATLFAKGNERSKVKDSENSAASVMFGDLQKSGYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BLU0</accession>
    <name>A0AAD5BLU0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Histone H3.2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_013949</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the histone H3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011856">
        <property type="protein sequence ID" value="KAI7725778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BLU0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22911">
        <property type="entry name" value="HFD_H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000078">
        <property type="entry name" value="Histone H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000044">
        <property type="entry name" value="Histone H3.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000164">
        <property type="entry name" value="Histone_H3/CENP-A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11426">
        <property type="entry name" value="HISTONE H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00622">
        <property type="entry name" value="HISTONEH3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00428">
        <property type="entry name" value="H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00322">
        <property type="entry name" value="HISTONE_H3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="12">
        <location>
            <begin position="44"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7725778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="136" mass="15300" checksum="E40EE3B8A8977A61" modified="2024-05-29" version="1">MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRFRPGTVALREIRKYQKSTELLIRKLPFQRLLREIAQDFKTDLRFQSSAVAALQEAAEAYLVGLFEDTNLCAIHAKRVTIMPKDMQLARRIRGERA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BPC6</accession>
    <name>A0AAD5BPC6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein PAF1-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_020166</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011434">
        <property type="protein sequence ID" value="KAI7727141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BPC6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016593">
        <property type="term" value="C:Cdc73/Paf1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007133">
        <property type="entry name" value="RNA_pol_II-assoc_Paf1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23188:SF15">
        <property type="entry name" value="RNA POLYMERASE II ASSOCIATED FACTOR PAF1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23188">
        <property type="entry name" value="RNA POLYMERASE II-ASSOCIATED FACTOR 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03985">
        <property type="entry name" value="Paf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="617"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="97"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="108"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="197"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7727141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="678" mass="76185" checksum="A9E1E1C9EB65144C" modified="2024-05-29" version="1">MAYRPFPPPPQPPIPNHGSLPPPPLAPLPQQRPNQYANNWGYPNPNPNPNSGFQQGGYPVPPLNPVVSNQQPQFNSYPPPPPPPESTYPPPPPPSYFPASQYSQPAQLQPPPPPPPPSSPPSSSVPPPPPAPPASPPPPPPPSSAPISNQAKESRQDGYKRNLDKDVGPSRQHERPVKTSNLPPVPVRKSNGGLGRVETEVERRVRKKKEMEKQKQEEKHRQQLKESQSKLLQKTQMMSSGSGSKPHGSITGSHMGDRRATSFLSGERVENRLKKPTTFLCKLKFRDELPDTTAQPKLLSIRRDKDRAAKYSITSLEKVNKPQLHVEPDLGIPLDLLDLSVYNAPKDEIHHIDPEDQILLQEDVSLTPIKKDGLKRKERPTDKGMSWLVKTQYISPISTDATRQSLTEKQAKDLREKRGNSILDNLSSRERKIKDIKASFEACKSRPVHATNKKLQPVEVLPLFPDFERWDDQFVVATFDGPPTVDSESYNKMDKSVRDAHESQAVMKSFVASTSDSAKPDKFLAYMVPSVGELSKDIYDEHEDISYTWVREYHWDVRGEDADDPTNFLVVFGESEARYMPLPTKLMLRKKRAREGKTTDEVEHFPVPASITVRQRSHVSVDMKETESFGGLKGRNSNPRHHGRMDMDDDANRHHHLHNDMDDGDMDQSSGAEYDISD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BPP5</accession>
    <name>A0AAD5BPP5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_031468</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011425">
        <property type="protein sequence ID" value="KAI7727170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BPP5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47880">
        <property type="entry name" value="OS05G0353300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47880:SF1">
        <property type="entry name" value="OS05G0353300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="409"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7727170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="501" mass="57156" checksum="EFFCB94183F50918" modified="2024-05-29" version="1">MGSLYNLNSVINFVSFNNKSPNFITPHVYNVKFLNSCSGVSFRNRNNIMYNNNIMCNGMRLGKFRLCKAVEMDTVVTSDDEDEMSEGFFEAIEALERMTREPSDVLEEMNSRLSARELQLVLVYFTQEGRDSWCALEVFEWLKKENKVDEETMELMVSLMCGWVKKVIEGKHEIGDVIDIVVDMECVGLKPNFSMIEKVISLYWDLGEKDAGVLFVKEVLRRGIGCEERGRKGGPTGYLAWKMMEDGSYRDAIKLVIEIKESNLKPEVYSYLIAMTAVVKELNEFSKALRKLKSFTKSGLISNLDAEDTHLIQNYQSTLLSDGVRLSNWVIEEGGPSFQGVVYERLLAMYICAGRGPEAEKQLWKMKLIGKEPDANLYDIVLAICASQKEIGSISRLLTRMEVTSLLNKKKTLTWLLRGYIKGSHYEDAAETVSKMLDLGVYPEFLDRAAVLQGLRRRINQTGNVEVYFNLCKQLSDAGLTGPCLIYMYIKKYKLWVIKML</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BRI5</accession>
    <name>A0AAD5BRI5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">phospholipid:diacylglycerol acyltransferase</fullName>
            <ecNumber evidence="9">2.3.1.158</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_001804</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a glycerophospholipid + a 1,2-diacyl-sn-glycerol = a monoacylglycerophospholipid + a triacyl-sn-glycerol</text>
            <dbReference type="Rhea" id="RHEA:14057"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:64615"/>
            <dbReference type="ChEBI" id="CHEBI:136912"/>
            <dbReference type="ChEBI" id="CHEBI:136913"/>
            <dbReference type="EC" id="2.3.1.158"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.158" evidence="9"/>
    <dbReference type="EMBL" id="JAMZMK010011234">
        <property type="protein sequence ID" value="KAI7728326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BRI5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008374">
        <property type="term" value="F:O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046027">
        <property type="term" value="F:phospholipid:diacylglycerol acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019432">
        <property type="term" value="P:triglyceride biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000160">
        <property type="entry name" value="Phospholipid:diacylglycerol acyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003386">
        <property type="entry name" value="LACT/PDAT_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11440">
        <property type="entry name" value="LECITHIN-CHOLESTEROL ACYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02450">
        <property type="entry name" value="LCAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="379"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="15"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00051335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00066405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7728326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="680" mass="75500" checksum="72374615D401E213" modified="2024-05-29" version="1">MATPIKPSTHFLTQTPPPPPPPPPLSGHCLRHPTHVTHLLCRQYSEEENNPNTIRIQNQLKKKKTTMIRNKKLWWILLFLYNAMPASFPQFVTEAISGPVPDPPGVKCLKQGLKVKHPVVFVPGIVTGGLELWEGHHCMDGLFRKRLWGGTFGEVYKRPSCWVQHMSLDNKTGMDPPGIRVRPVNGLVAADYFAPGYFVWAVLIANLARVGYEEKNMYMAAYDWRLSFQNTELRDQTLSRIKSNIELMVATNGGKKAVIIPHSMGVIYFLHFMKWVEAPAPMGGGGGPDWCAKHIKAVMNIGGPFLGVPKAVAGLFSAEAKDIASVRALAPGVLDSDLFQIQTLQHIMRMSRTWDSTMSMIPKGGDTIWGGLDWSPEEGYSPSKSKHGKNRSESSTLNESTGEVTHANYGRIISFGRDVADAPSSEIERIEFRGAVKGKNVANNTCRAVWTEYHDMGFGGIKAVAEYKVYTAGEIVDLLEFVAPKMMERGSAHFSYGIADNLDDPKYTHHKYWSNPLETKLPNAPDMEIYSMYGVGIPTERAYVYKLTPAAECYIPFQIDTSAKDKNEDGCLKDGVYTVEGDETVPALSAGYMCAKGWRGKTRFNPSGIKTYVREYDHNPPSNFLEGRGTQSGAHVDIMGNFQLIEDVIKVAAGATGEELGGDQVYTDIFEWSEKINLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BS96</accession>
    <name>A0AAD5BS96_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_001068</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011193">
        <property type="protein sequence ID" value="KAI7728550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BS96"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050667">
        <property type="entry name" value="PPR-containing_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47939">
        <property type="entry name" value="MEMBRANE-ASSOCIATED SALT-INDUCIBLE PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47939:SF4">
        <property type="entry name" value="PENTACOTRIPEPTIDE-REPEAT REGION OF PRORP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13812">
        <property type="entry name" value="PPR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="103"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="138"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="173"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="243"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="278"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="313"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="378"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="38"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7728550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="502" mass="57399" checksum="42752772DB5CC4BB" modified="2024-05-29" version="1" fragment="single">TPSSFTQLLRRPSPKLIPNPKQQPLRNITHDSCSNDSNNNINNNNNNPLHPNDVVKQICLTIRSKPRWENTLLSDFPTVTFLDPNKAVNAAKTFLDFTKYVPDSVGFESYVRCLCESGMVEDALQVFDEMRKRGVCPLLETWNCVLRGCIKDGRVDVVWKLYGEMMELGVGVDVDTVDCLIQAFCLEKNVSKGYELLRQMLDDGYVPNKVSFDKLVFDFILDRKYPRVSALLHIMIAKGVNPDLYTYQQIVHGLCLKRMQREGLRIFNDLKDRGYAPDRIMYTTMIHGLCKIKWIGEARKLWFEMIQKGIRPNLYTYNTLLYGYFKIGDIENALNLYKEMKDKGYGETLIAYNTMINGRLEDGLNLLHELVDHGLRPSSASYGPLIEKLYETKRIDEAKNLWNEMQEKGVEPASVCINDDVIIGLSNKGYVAAGIYWLAYMVKNRLLPRQKTFENLIGVLSQKDMLDVGQIALGHMFKIGAANPLSPRIVILWECGYCIKGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BSE0</accession>
    <name>A0AAD5BSE0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">T-complex protein 1 subunit gamma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_019088</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. Known to play a role, in vitro, in the folding of actin and tubulin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011166">
        <property type="protein sequence ID" value="KAI7728717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BSE0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005832">
        <property type="term" value="C:chaperonin-containing T-complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03337">
        <property type="entry name" value="TCP1_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000037">
        <property type="entry name" value="T-complex protein 1 subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000073">
        <property type="entry name" value="T-complex protein 1 subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000005">
        <property type="entry name" value="T-complex protein 1 subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012719">
        <property type="entry name" value="Chap_CCT_gamma"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053374">
        <property type="entry name" value="TCP-1_chaperonin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054827">
        <property type="entry name" value="thermosome_alpha"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02344">
        <property type="entry name" value="chap_CCT_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041082">
        <property type="entry name" value="thermosome_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041083">
        <property type="entry name" value="thermosome_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 9" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="517"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="521"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7728717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="545" mass="59545" checksum="BA39CF0C4F1DF611" modified="2024-05-29" version="1">MQSPVLVLKDSLTRESGSKVHHANIQASKAVADIIRTTLGPRSMLKMLLDAQGGIVVTNDGNAILRELDIAHPAAKSMIELSRTQDEEVGDGTTSVIVLAGEMLHVAEAFIDKKYHPTVICRAYNKALEDALAVLDQISMSIDVNDRSMMLGLVKSCIGTKFTSQFGDLIADLALDATTTVGVDLGQGIREVDIKKYIKVEKIPGGQLEDSKVLKGVMFNKDVVVPGKMKRKIVNPRIILLDCPLEYKKGENQTNAELVREEDWAVLLKMEEEYIENLCVQILKFKPDLVITEKGLSDLACHYFSKAGVSAIRRLRKTDNNRIAKACGAVIVNRPDELQESDIGTGAGLFEVKKIGDEFFSFIVDCKDPKACTVLLRGASKDLLNEVERNLQDAMSVARNILKHPKLVPGGGATELTWPYEAAAVAFEAIPRTLAQNCGVNVIRTMTALQGKATKDVMHATGENAWTGIDGNSGEIADMKELKIWDAYNVKAQTFKTAIEAACMLLRIDDIVSGIKKRQAPGSNQPSKPTIETEGDADNEQMIPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BWL5</accession>
    <name>A0AAD5BWL5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Beta-fructofuranosidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_020880</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 32 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010655">
        <property type="protein sequence ID" value="KAI7730775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BWL5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18624">
        <property type="entry name" value="GH32_Fruct1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.560:FF:000002">
        <property type="entry name" value="Beta-fructofuranosidase, insoluble isoenzyme CWINV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.560">
        <property type="entry name" value="Exo-inulinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.115.10.20">
        <property type="entry name" value="Glycosyl hydrolase domain, family 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050551">
        <property type="entry name" value="Fructan_Metab_Enzymes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001362">
        <property type="entry name" value="Glyco_hydro_32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013189">
        <property type="entry name" value="Glyco_hydro_32_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013148">
        <property type="entry name" value="Glyco_hydro_32_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023296">
        <property type="entry name" value="Glyco_hydro_beta-prop_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31953">
        <property type="entry name" value="BETA-FRUCTOFURANOSIDASE, INSOLUBLE ISOENZYME CWINV1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08244">
        <property type="entry name" value="Glyco_hydro_32C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00251">
        <property type="entry name" value="Glyco_hydro_32N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00640">
        <property type="entry name" value="Glyco_32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75005">
        <property type="entry name" value="Arabinanase/levansucrase/invertase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042155236" description="Beta-fructofuranosidase" evidence="5">
        <location>
            <begin position="32"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 32 N-terminal" evidence="6">
        <location>
            <begin position="56"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 32 C-terminal" evidence="7">
        <location>
            <begin position="349"/>
            <end position="549"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7730775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="556" mass="63572" checksum="A392D3B7C08B8C59" modified="2024-05-29" version="1">MMRVFNRCVWSLALLICMSMLNNFHERGVMAFHDVHLKYQTNNIEKVKQAYRTGYHFQPKKNWMNGPMFYKGLYHLFYQYNPKGAVWGNIVWAHSVSKDMINWIPLEPAIVPSKPFDKYGCWSGSATILPGDKPVIIYTGIINEKPEPGHQVQNYAIPANYSDPYLRTWIKPDNNPIIKPIHENVSSFRDPTTAWFNNGHWKLIVGSKNNRRGITYLYRSRDFIKWTKAKHPFHTKNDVGMWECPDFYPISSLGTNGLDTSALGDNDKYVPYNTSVDRWAGLRYDYGNFYASKTFFDPIKKRRILWGWANESSTRSEDIAKGWAGIQLIPRKVWLDPNGHQLLQWPITELESLRGQKIELSNVKLNKGDTIEVKGINVVQADVDVTFTFSSLDKAEPYDEKWRKFPPEDLAKSICGIKGATVQGGLGPFGLLTLASSKLEEYTPVFFRIFKTTDKKYKVLFCSDATPSSLNKNEYKPSFGGFVDVDLTTKKLSLRSLIDHSVVESFAEGGKTVISSRVYPTLAVNENAHLHLFNNGSEIVTIERLSAWSMKTARIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BY93</accession>
    <name>A0AAD5BY93_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Vacuolar protein sorting-associated protein 51 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_000472</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Acts as component of the GARP complex that is involved in retrograde transport from early and late endosomes to the trans-Golgi network (TGN).</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Component of the Golgi-associated retrograde protein (GARP) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">trans-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the VPS51 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010421">
        <property type="protein sequence ID" value="KAI7731600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BY93"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990745">
        <property type="term" value="C:EARP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000938">
        <property type="term" value="C:GARP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048193">
        <property type="term" value="P:Golgi vesicle transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007041">
        <property type="term" value="P:lysosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014812">
        <property type="entry name" value="Vps51"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15954">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 51 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15954:SF4">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 51 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="2" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="2" id="KW-0653">Protein transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="210"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="528"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="537"/>
            <end position="548"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU368010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7731600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="685" mass="77749" checksum="B6D067724483004E" modified="2024-05-29" version="1">MAADDVSVDDKVKRTRDLLSSFYSQDASQISPPSNTTSRFATLDTINTTSFDADQYMNLLVHKSNLEGLLQRHVEMAAEIKNLDTDLQIMKNNIVGMEVNMEQLLDKIMSVQSKSDGVNTLLFEKREHIEKLHRTRNLLRKVQDCKKASEEVMLVIVNKLEDKVSSDSESIQARAEAVMLLKQLDFPVEKLESKLLEKLEQFLEELDLSKDTSSDSANPHESINEGIESDSIPPRPEVPTREFVEAVHAYHVIFPDSEQHLVKLMHDLTTRHFEAAKQQIQEQVSSAKLSNRLRFIWTDVLSIDEVLPEAGLQDFAYEAARDVLKQYVTSLFSYLLHEISDALLQIQKDGIGEEYPLRAALEASKNALIQGSTKVLSDIRYLLDEDLGLVLKLRDMIISWVQEGFQTFFTQLNGQLLLLSGKDVPTDKVSSGLVLVISQLSVFIERDAISRITEEIGSSLLGGSYEYGSSAFYISMRTQRISVLLRKRLTTPNWIKHKEPREVHMFVDLFLQELGAVGSEVKQILPQGLTRRHSRTESNGSTSSSRSNQLRDNRSNTNRARSQLLETHLAKLFKQKMEIFTKIEHTQESVVMTIVKRCLKSLQEFVRLHTFNRSGFQQIQLDMQYLRITLKDNAEDEAAVEFLLDEVIVAAAERCVDPSPLEPAILDRLVQAKLAKTMDQEPVAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BYM5</accession>
    <name>A0AAD5BYM5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">PUM-HD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_013478</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Sequence-specific RNA-binding protein that regulates translation and mRNA stability by binding the 3'-UTR of target mRNAs. Binds the APUM-binding elements (APBEs) in the 3'-UTR mRNA sequence of CLV1, PNH, WUS and FAS2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010608">
        <property type="protein sequence ID" value="KAI7730864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BYM5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07920">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000004">
        <property type="entry name" value="Pumilio homolog 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012940">
        <property type="entry name" value="NABP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033133">
        <property type="entry name" value="PUM-HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033712">
        <property type="entry name" value="Pumilio_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001313">
        <property type="entry name" value="Pumilio_RNA-bd_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537:SF187">
        <property type="entry name" value="OS04G0276200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537">
        <property type="entry name" value="RNA BINDING PROTEIN PUMILIO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07990">
        <property type="entry name" value="NABP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00806">
        <property type="entry name" value="PUF"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00025">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50302">
        <property type="entry name" value="PUM"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50303">
        <property type="entry name" value="PUM_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="PUM-HD" evidence="9">
        <location>
            <begin position="443"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="7">
        <location>
            <begin position="463"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="7">
        <location>
            <begin position="499"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="7">
        <location>
            <begin position="535"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="7">
        <location>
            <begin position="571"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="7">
        <location>
            <begin position="607"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="7">
        <location>
            <begin position="644"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="7">
        <location>
            <begin position="680"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="7">
        <location>
            <begin position="716"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="229"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="255"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00055193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7730864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="808" mass="88212" checksum="DF4EF9901C38BF53" modified="2024-05-29" version="1">MMMTDSYSKMMSDIGMRSSNDHEFEINMYRSGSAPPTVDGSLNAVAGLFDGGVLEHDLRSDPAYLDYYHANVNLNPRLPPPLLSKERFSQRVQAGSSAIGDMRKVKQDNESNVSEWGGNGLIGLRGLGQPSRPASRNAFDDNVDLSDSHFGHLQGMSSGSHTYASMLGSSLSRNTTPDPQLVARTPSPLVPSDLVSGFSNLNLSTNGMVDEETQLQARVQKAAHLAKGSNQYMGKSGGPRIGQGSPVNSYMKGPTVTNGSGGSPSRHQNLDSPSSSFYGLGGGGGGYVMNPTSPMMMANHILPSLYDNGAVAAAMGVSAMDSRGMGGGFALEPSRLMDPSLYLQNLRSNEYMSALNDATIDRELLAIQKAYLAGLLSPQKSPYGLNHGYYGSPGYGLGMSYPGSPLGSPLLPNSAFGPGSPARHVDRSLRFSPGIRNIVGESFASSLLDEFKSNKTKCFELSEIVGHVVEFSSDQFGSRFIQQKLETATTEEKNMVFNEIMPQAYSLMTDVFGNYVVQKFFEHGSATQIRELADQLTGHVLTLSLQMYGCRVIQKAIEVVDLDQQTKMVTELDGHVMRCVRDQNGNHVIQKCIECVPENAIHFIISTFYDQVVTLSTHPYGCRVIQRVLEHCHNPKTQSIVMDEILKSINMLAQDQYGNYVVQHVLEHGKPHERNCIIDQLTGQIVQMSQQKFASNVVEKCLTFGTPEQRQTLVTEMLGTTDENEPLQVMMKDQFANYVVQKVLETCDDQQLEMILNRIKVHLNALKKYTYGKHIVARVEKLVAAGERRIGAMAGYAAAATVASASTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C022</accession>
    <name>A0AAD5C022_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phorbol-ester/DAG-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_011336</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010377">
        <property type="protein sequence ID" value="KAI7731813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C022"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004146">
        <property type="entry name" value="DC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053192">
        <property type="entry name" value="Vacuole_Formation_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32410">
        <property type="entry name" value="CYSTEINE/HISTIDINE-RICH C1 DOMAIN FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32410:SF216">
        <property type="entry name" value="PHORBOL-ESTER_DAG-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03107">
        <property type="entry name" value="C1_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="5">
        <location>
            <begin position="473"/>
            <end position="531"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7731813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="613" mass="72069" checksum="A3139EC4B0B9782D" modified="2024-05-29" version="1">MVEEPLVGKCEMCKEEIYSFHQSYYSCKDCDYSLHKFCAEMPTTQQNHPFHPGHNLTLSQKFQRHDPDVKFKETSDSSWYCQVCKIKRTMFYNYHCYICKFDMDIVCATMSQQKMNHPSHPHQLQRDLNWMTSHCYACEEKHSGVFYHCTTCLWFRIHLDCALLPAKLLIQKSTNGSFSHLHLLTLYYSPFPYIEQKAKFFPSCRVCDRSFVSSRNRWYYGCHKCRYYIHVRCATSRPGRLDRTYKNFEDHDHPNLIHCPFPDESANLLTHQFVNKGELVLNEMIDGQLFSHQHPLILVKTLLNESVSLNDPMKRVELLCDGCVRPITALPFYKCSQHNCGFVLHEWCTRLPSEILDHPDHPEHKLVLLPKMPENLLGTFRCAVCRLSCNGFAYGCIECKYYVDINCGFIPDAITHEAHPNHLLLRFKASPHQPKQGCKACDSYIEAPLRFHCPTCDFSLHTECALSIPRTIRHKFDKHPLSLRYYPVENYSSDYFCEICEDVLNPREWFYHCNMCAWSIHTECAPVKLECERFTSSEFNRSIFAYYNVKFGGRYKMRDHPHLVTFVQGIKDDGQCIVCHEQLQYERIFKCFECKFALHFGCEEPDVLEIPQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C125</accession>
    <name>A0AAD5C125_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PCI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_029833</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the proteasome subunit S11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010096">
        <property type="protein sequence ID" value="KAI7733040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C125"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054179">
        <property type="entry name" value="PSD13_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035298">
        <property type="entry name" value="PSMD13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10539">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10539:SF7">
        <property type="entry name" value="PROTEASOME COMPONENT (PCI) DOMAIN, 26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22037">
        <property type="entry name" value="PSD13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0647">Proteasome</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="3">
        <location>
            <begin position="1"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7733040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="217" mass="25179" checksum="E3EAC22EC0E3CF73" modified="2024-05-29" version="1">MATLQYLESLRTTHPELSKWYNTLADLYQRKLWHQLTLKLEQFVALTIKSLLGTKVEWLYYILEAFNSGDLVRYQELCRVHNASLFAQSALVENEKKLLEKINILCLMEIIFSRPSEDRTIPLGIIAERTKLTVEDVEYLLMKSLSVHLIEGIIDQVDGTVYVSRVQPRVLGISQIKLLPDHLCNILLKAQRRAHDRVQKEIRILTMTGYNLRVSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C148</accession>
    <name>A0AAD5C148_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein DETOXIFICATION</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_033490</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010092">
        <property type="protein sequence ID" value="KAI7733085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C148"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044644">
        <property type="entry name" value="DinF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42893:SF4">
        <property type="entry name" value="PROTEIN DETOXIFICATION 42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42893">
        <property type="entry name" value="PROTEIN DETOXIFICATION 44, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="61"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="108"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="147"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="185"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="340"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7733085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="410" mass="44266" checksum="F16A66734C3A7372" modified="2024-05-29" version="1">MDDLGVEIAKIALPAALALTADPIASLVDTAFIGQIVTESPDSESLVTSDKKKRIPSASSALIIGSVLGFLQAAFLIIGAKPVLGFMGIKSDSPMLHPAQQYLKLRSLGAPAVLLSLAMQGVFRGFKDTKTPLYATGDVTNIILDPIFMFVFDLGVSGAAIAHVLDFEKIDFVSPTSKHLQFGRFLKNGFLLLMRVMAVTFCVTLSASMAARQGSTTMAAFQVCLQVWLATSLLADGLAVAGQGSGYTSGQDCLQKMLVSSISLASAYRYHNSTKTKFFTDYKPILNGSIRFPYQFVAATQPINALAFVFDGVNFGASDFAYAAYSMVCKKGLQNKIKTGLKGMKVLVAMISIISLFIASSHGFVGLWIALTFYMSLRAFAGFWRKTMSPNTSFRRYQPKRAGSWSRAMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C2R0</accession>
    <name>A0AAD5C2R0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Fe/B12 periplasmic-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_012184</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009766">
        <property type="protein sequence ID" value="KAI7734281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C2R0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002491">
        <property type="entry name" value="ABC_transptr_periplasmic_BD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38360:SF1">
        <property type="entry name" value="F12P19.7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38360">
        <property type="entry name" value="OS03G0120000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01497">
        <property type="entry name" value="Peripla_BP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53807">
        <property type="entry name" value="Helical backbone' metal receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe/B12 periplasmic-binding" evidence="1">
        <location>
            <begin position="51"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7734281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="295" mass="33287" checksum="78DEDCDAB9A67A05" modified="2024-05-29" version="1" fragment="single">LLGLMSTMKGITSEKVASPCLLKLYNEGALQMLNNSEPQHFSQYAAHFIGLNDDQETQSCNYITFSPMDEQAPLQRAEWIKYLGSFADNEVRANEIYNVVKSNYMCLVNTTASKNLKPVVAWMDFNDGIWSFTDEAYKLKYIEDAGGENLDESINKITYDPSFIEDLEALHAILCTVDVVIDGTPTRDPFSYNATLFLQNLNIDDHSCFSFLSSQSVWRYDKRLSSDLALDWYDGAVSQPQLVLADLREALFPSGDYTTTYFRNLLQEEQPISLGPESCTRDTFAAMEPTIIACP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C3Y1</accession>
    <name>A0AAD5C3Y1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Sugar phosphate transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_005768</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009946">
        <property type="protein sequence ID" value="KAI7733551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C3Y1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004853">
        <property type="entry name" value="Sugar_P_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050186">
        <property type="entry name" value="TPT_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11132">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03151">
        <property type="entry name" value="TPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="41"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="70"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="109"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="142"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="169"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="193"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="225"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="257"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="295"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="319"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="Sugar phosphate transporter" evidence="7">
        <location>
            <begin position="51"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7733551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="343" mass="37793" checksum="8BF2A31B439B2A90" modified="2024-05-29" version="1">MLPSTDRMTLPVSDPPPPPPRGGAVAGDQRLMKGSTMTSRGAYAAVSYMSCAVMLVLFNKAALSSYSFPSANVITLFQMICSCCFLYVLRRWKLITFSSGDADNSTKFVSLQTLIHTSPLALTYLMYMLATMESVRGVNVPMYTTLRRTTVVFTMFVEFILVGQRYSRSVIGSVALIVFGAFVAGSRDLSFDSYGYSIVFLSNITTAIYLATISRIGKSSGLNSFGLMWCNGIMCLPVLLFWTFIRGDLRTTMTFPYLFAPGFLIVLLLSCMLAFLLNYSIFLNTTLNSAVTQTICGNLKDIFTIALGWMFFGGLPFDLLNVIGQILGFVGSGLYAYFKLFGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C4C7</accession>
    <name>A0AAD5C4C7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_025068</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009694">
        <property type="protein sequence ID" value="KAI7734540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C4C7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33738">
        <property type="entry name" value="EMB|CAB82975.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33738:SF21">
        <property type="entry name" value="TPRXL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="28"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7734540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="127" mass="13409" checksum="D7BD7A2AB6A47906" modified="2024-05-29" version="1">MENMKQQTGGSSSTSFTETLFGPKDGRSSSSGIFGSAFGPSSTGVGHTGSSNKQSFGNPKESRTDCKTQQVTETAYSSSIYYGGQDVYPPTNNNNCPHRAGHRGGGDAYPNGSSAARGNWWQGSLYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C514</accession>
    <name>A0AAD5C514_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Sodium/calcium exchanger membrane region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_008333</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009492">
        <property type="protein sequence ID" value="KAI7735393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C514"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009705">
        <property type="term" value="C:plant-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015369">
        <property type="term" value="F:calcium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.30">
        <property type="entry name" value="NCX, central ion-binding region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004713">
        <property type="entry name" value="CaH_exchang"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004837">
        <property type="entry name" value="NaCa_Exmemb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044880">
        <property type="entry name" value="NCX_ion-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31503">
        <property type="entry name" value="VACUOLAR CALCIUM ION TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31503:SF42">
        <property type="entry name" value="VACUOLAR CATION_PROTON EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01699">
        <property type="entry name" value="Na_Ca_ex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0050">Antiport</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="53"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="9">
        <location>
            <begin position="3"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7735393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="120" mass="13407" checksum="F2AAA89529CCE97B" modified="2024-05-29" version="1" fragment="single">MFALHKRKIHVLKYSLLGSIISNILLVLGSSLLCGGIANINKEQKFDHKQADANTLLLLLGLLCHIAPLLFNLHSMDLPISDRTSDIQLSRSCSIVMLIAYVAYLFFQLKTHNQPFGSEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C533</accession>
    <name>A0AAD5C533_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Small ribosomal subunit protein uS7 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_033024</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uS7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009488">
        <property type="protein sequence ID" value="KAI7735423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C533"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14867">
        <property type="entry name" value="uS7_Eukaryote"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.455.10:FF:000002">
        <property type="entry name" value="40S ribosomal protein S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.455.10">
        <property type="entry name" value="Ribosomal protein S7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000235">
        <property type="entry name" value="Ribosomal_uS7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020606">
        <property type="entry name" value="Ribosomal_uS7_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023798">
        <property type="entry name" value="Ribosomal_uS7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036823">
        <property type="entry name" value="Ribosomal_uS7_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005716">
        <property type="entry name" value="Ribosomal_uS7_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003106">
        <property type="entry name" value="PRK04027.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01028">
        <property type="entry name" value="uS7_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11205">
        <property type="entry name" value="RIBOSOMAL PROTEIN S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00177">
        <property type="entry name" value="Ribosomal_S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002122">
        <property type="entry name" value="RPS7p_RPS7a_RPS5e_RPS7o"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47973">
        <property type="entry name" value="Ribosomal protein S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00052">
        <property type="entry name" value="RIBOSOMAL_S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS7" evidence="5">
        <location>
            <begin position="44"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7735423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="205" mass="22835" checksum="95D7F732CC38C55E" modified="2024-05-29" version="1">MEVLPPVAEDGKSHSDVLLFNRWTYDDVQVSDLSVEDYITATASKHPIYMPHTAGRYQAKRFRKAQCPVVERLTNSLMMHGRNNGKKLMAVRIVKHAMEIIHLLTDANPIQIIVDAVINSGPREDATRIGSAGVVRRQAVDISPLRRVNQAIYLLTTGARESAFRNVKTIAECLADELINAAKGSSNSYAIKKKDEIERVAKANR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C676</accession>
    <name>A0AAD5C676_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_014680</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010009528">
        <property type="protein sequence ID" value="KAI7735218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C676"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008420">
        <property type="term" value="F:RNA polymerase II CTD heptapeptide repeat phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10845">
        <property type="entry name" value="DSRM_RNAse_III_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000035">
        <property type="entry name" value="RNA polymerase II C-terminal domain phosphatase-like 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014720">
        <property type="entry name" value="dsRBD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039189">
        <property type="entry name" value="Fcp1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23081:SF24">
        <property type="entry name" value="RNA POLYMERASE II C-TERMINAL DOMAIN PHOSPHATASE-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23081">
        <property type="entry name" value="RNA POLYMERASE II CTD PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00035">
        <property type="entry name" value="dsrm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00358">
        <property type="entry name" value="DSRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50137">
        <property type="entry name" value="DS_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DRBM" evidence="9">
        <location>
            <begin position="535"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="402"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="614"/>
            <end position="636"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7735218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="636" mass="71590" checksum="7FF429746722F8EA" modified="2024-05-29" version="1">MSHLGFNNINNNNKSSVVNVYHGDISLGELDIIPTGDKSFHFPNNVINIHHISPISERCSPLSVLLAISSSSVRCKLESLSSVVKQPLLDLHASCFYELKTAVVLIGDEEVHLVAMPSKLKRFPCFWCCVVPSQLYNACLWMLNTRCLSIVFDLDETLIVANTMRSFEDRIEVLTGWIGQESDPVRDMYHNERRTPDMEKVRAPRFGPTWGFRCASMLPQIRDTSVLVRLRPAWEDLRSYLIAKGRKRFEVMVLTLEKNHETRFDRARKSLLNVFKDRNCHPRMAMVIDDRLKVWEEKDQPRVHVVPAFTPYYAPQAETANAVPVLCVARNVACNVRGGFFKEFDENFLRRVSEYFYEDEVLNLPSAPDVCSYLMPEDASFVPNGNTSAPMVEGMNASEVAHRLNQQDSRNVDQKSERSQPLIPSASIYGPPSFRSMIPSDKPSLLGPIQAPDILNKQRAGYYPPSHGTASSFSAGFQPLVSEAKRDEVFHGYGLPKKNLPQIGQLSGVAHNQPYLNNKRGPTDSGNLNPPQPSLSIGVLQEIGQRCGSKVEYRSVFSSTREWRFSYEVLLTGEKVGFGMGNTRKDAQQQAADNALRSLADQYVSYLVSRSKTDNKDLDSQLPEGENGFLWDSDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C6D2</accession>
    <name>A0AAD5C6D2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Jacalin-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_030683</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the jacalin lectin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009634">
        <property type="protein sequence ID" value="KAI7734841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C6D2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09612">
        <property type="entry name" value="Jacalin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.100.10.30">
        <property type="entry name" value="Jacalin-like lectin domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001229">
        <property type="entry name" value="Jacalin-like_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033734">
        <property type="entry name" value="Jacalin-like_lectin_dom_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036404">
        <property type="entry name" value="Jacalin-like_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46506">
        <property type="entry name" value="OS05G0143600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01419">
        <property type="entry name" value="Jacalin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00915">
        <property type="entry name" value="Jacalin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51101">
        <property type="entry name" value="Mannose-binding lectins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51752">
        <property type="entry name" value="JACALIN_LECTIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0430">Lectin</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Jacalin-type lectin" evidence="3">
        <location>
            <begin position="15"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="Jacalin-type lectin" evidence="3">
        <location>
            <begin position="162"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7734841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="315" mass="34208" checksum="C520EDB7609E9A4A" modified="2024-05-29" version="1">MNIPHAQTNTKRAGFIRVGLWGKQSGGPQNEWSFGLDQGQKLRKITIDHGDDVIYSLMFTTEACGVFNTSNKFGGWNGGQTVTLDSDEEIVGIKGSIGTKAPYTIVSSLSFVTNKTTHGPFGGATNSEFSLPLEMGSLVGFYGLAGYYIDGIGVYLKPHRETMKIGTWGKTQPAGPQNNWSFQLEPTYHLTKITIDHGDLIYSLMFTAQCGELTNTSEKMGGWQGGEKVTEITFDEDEEINCISGTVALSRGTYAGNTVISSISFMTNKKVYGPFGNVRGTPFTVPWEDGSFAGFYGLAGYYIDSIGVYLKATNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C6V6</accession>
    <name>A0AAD5C6V6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PIPK domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_017114</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009434">
        <property type="protein sequence ID" value="KAI7735830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C6V6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000285">
        <property type="term" value="F:1-phosphatidylinositol-3-phosphate 5-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17300">
        <property type="entry name" value="PIPKc_PIKfyve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.810.10:FF:000001">
        <property type="entry name" value="1-phosphatidylinositol 3-phosphate 5-kinase FAB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.800.10:FF:000007">
        <property type="entry name" value="Putative 1-phosphatidylinositol-4-phosphate 5-kinase/ zinc ion binding family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.810.10">
        <property type="entry name" value="2-Layer Sandwich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.800.10">
        <property type="entry name" value="Phosphatidylinositol Phosphate Kinase II Beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044769">
        <property type="entry name" value="PIKfyve_PIPKc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027483">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002498">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027484">
        <property type="entry name" value="PInositol-4-P-5-kinase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45748">
        <property type="entry name" value="1-PHOSPHATIDYLINOSITOL 3-PHOSPHATE 5-KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45748:SF14">
        <property type="entry name" value="1-PHOSPHATIDYLINOSITOL-3-PHOSPHATE 5-KINASE FAB1C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01504">
        <property type="entry name" value="PIP5K"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00330">
        <property type="entry name" value="PIPKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51455">
        <property type="entry name" value="PIPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PIPK" evidence="6">
        <location>
            <begin position="238"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="58"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="66"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7735830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="567" mass="64357" checksum="D2C0FE7A25928D2A" modified="2024-05-29" version="1">MSVAEIDVLELNRLRHSLMIDLHVWDQRLQSLNSIFKRSSSLEVKHRRAYSTLEAIVDHSHASADPESDQIDSKDETDESNQIDLKSTSSDQFSECNASFLSDKIDSAWTGPLTRPEIPQIRRLISTPARVYSFDSAITLQERMKYQQKVKANDNIQRSYSQVIPNEDQRLDFMWRTKPSFISRTSLLPDGARLIRGQNVVIAVYENEPTSIISYALASKEYEDWFTDKPSLSKHHSVASKLTAWQSFGSNNDYDYMSYGNYGSEETSTAIGSLFSEPKSSHHLAIQFEDESGPGGKVKFSVTCYFAKQFDVLRKKCCPSETDYLRSLSRCKKWMAQGGKSGADFAKSLDDRFLIKGVTKTELDSFEEFAPQYFKYLTDSLNTGSPTCLAKIVGIYQVNVKHAKGGKESKSIVMVMENLFFKKNISRVYDLKGSARSRYNSDITGTMLDMNLLEALRTNPLFLGSKAKRNLERAVWNDTSFLASIDVMDYSLLVGVDEGSKELVLGIIDFMRQYTWDKQLETWVKASGILGGPKNAAPTIISPKQYKRRFRKAMTSYFLTVPDQWSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C816</accession>
    <name>A0AAD5C816_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dynein light chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_016103</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the dynein light chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009276">
        <property type="protein sequence ID" value="KAI7736430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C816"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005868">
        <property type="term" value="C:cytoplasmic dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.740.10:FF:000004">
        <property type="entry name" value="Dynein light chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.740.10">
        <property type="entry name" value="Protein Inhibitor Of Neuronal Nitric Oxide Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037177">
        <property type="entry name" value="DLC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001372">
        <property type="entry name" value="Dynein_light_chain_typ-1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11886">
        <property type="entry name" value="DYNEIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11886:SF78">
        <property type="entry name" value="DYNEIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01221">
        <property type="entry name" value="Dynein_light"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01375">
        <property type="entry name" value="Dynein_light"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54648">
        <property type="entry name" value="DLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="1" id="KW-0243">Dynein</keyword>
    <keyword evidence="1" id="KW-0493">Microtubule</keyword>
    <keyword evidence="1" id="KW-0505">Motor protein</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU365010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7736430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="95" mass="10650" checksum="B755ADCA66B27F99" modified="2024-05-29" version="1">MLEGKALVEDTDMPMKMQVQAMAYASQALDLYDVLDCKSIAAHIKKEFDKKYGNGWQCVVGSNFGCFFTHTKGTFIYFTLETLNFLIFKGASSLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C8D9</accession>
    <name>A0AAD5C8D9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Alpha-D-phosphohexomutase alpha/beta/alpha domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_022391</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009398">
        <property type="protein sequence ID" value="KAI7735979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C8D9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009570">
        <property type="term" value="C:chloroplast stroma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004615">
        <property type="term" value="F:phosphomannomutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.120.10">
        <property type="entry name" value="Alpha-D-Glucose-1,6-Bisphosphate, subunit A, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.50">
        <property type="entry name" value="Alpha-D-phosphohexomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005844">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016055">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I/II/III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036900">
        <property type="entry name" value="A-D-PHexomutase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050060">
        <property type="entry name" value="Phosphoglucosamine_mutase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42946:SF1">
        <property type="entry name" value="PHOSPHOGLUCOMUTASE (ALPHA-D-GLUCOSE-1,6-BISPHOSPHATE-DEPENDENT)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42946">
        <property type="entry name" value="PHOSPHOHEXOSE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02878">
        <property type="entry name" value="PGM_PMM_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55957">
        <property type="entry name" value="Phosphoglucomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53738">
        <property type="entry name" value="Phosphoglucomutase, first 3 domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="4">
        <location>
            <begin position="95"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7735979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="432" mass="46473" checksum="4A6AC842F77D094B" modified="2024-05-29" version="1">MAEMTGKIVQNLPSAQCCQQNRQFGSLYQRDQCAQFKNNFLLFQVRKLTWTGISAMQLHNTSNVSKRVVYCNASPSATVVPSLGKVDFLKLQNGSDIRGVAVDGVAGEPITLTEPVTQAISAAFAAWLLDKKKADSSKRLKISVGHDSRISAQKLQDAASRGIAGADFDVVQYGLASTPAMFNSTLTKNDDILCPVDGAIMITEKPLEGFHIVVDAGNGAGGFFAGNVLEPLGAITSGNKAAMKAITQAVLDNKADLGIIFDTDVDRSAAVDSTGRGKHHRFKRGYKNVIDEAIRLNSVGEESHLAIETSGHGALKENHWLDDGAYLMVDQNHSDLNGGSFREYGEAVLKHLENTSESDPKLQKAPVNYEGVRVSGYGGWFLLRLSLHDPVLPLNIEAPSNEDAVKLGLAVLSAVNEFSALDVSALTKFVQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C937</accession>
    <name>A0AAD5C937_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">MYB-CC type transcription factor LHEQLE-containing domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_005398</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009150">
        <property type="protein sequence ID" value="KAI7736985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C937"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025756">
        <property type="entry name" value="Myb_CC_LHEQLE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046955">
        <property type="entry name" value="PHR1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31499">
        <property type="entry name" value="MYB FAMILY TRANSCRIPTION FACTOR PHL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31499:SF2">
        <property type="entry name" value="MYB-RELATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14379">
        <property type="entry name" value="Myb_CC_LHEQLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MYB-CC type transcription factor LHEQLE-containing" evidence="2">
        <location>
            <begin position="19"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7736985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="93" mass="10691" checksum="EF5094B578659274" modified="2024-05-29" version="1">MIGNHMSNSSVKPQANRNLQINEARQMQTEVQKRLHEQREVQRHLQLRIEAQGKYLEAILEMAQETAGRENLSEVGRSKPASKLSSSHHRHTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CBJ0</accession>
    <name>A0AAD5CBJ0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_017614</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008990">
        <property type="protein sequence ID" value="KAI7737599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CBJ0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7737599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="195" mass="21284" checksum="53B5B54FCE2D5700" modified="2024-05-29" version="1">MQHVMQSSATPLTAASTPSDTITPPVKSQSETSTKRSLMESEVGVTYYCNNWTESLLFNIMQMCSGDGFGSTAYRRGATAVSGSDSSGDFTVAQEALISTDQKYRAETGHTWAWDWKGFPAQEEAWNNFGTLTQLLRGLLVYKVLGESTTLANQMQPYEITSTVVGESKLFQDSLTSFLGYKHKDSKKAATGITA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CBT1</accession>
    <name>A0AAD5CBT1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">P-type ATPase A domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_013763</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008675">
        <property type="protein sequence ID" value="KAI7739098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CBT1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019829">
        <property type="term" value="F:ATPase-coupled monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02079">
        <property type="entry name" value="P-type_ATPase_HM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000002">
        <property type="entry name" value="Copper-transporting ATPase 1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000043">
        <property type="entry name" value="Putative cadmium/zinc-transporting ATPase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051014">
        <property type="entry name" value="Cation_Transport_ATPase_IB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027256">
        <property type="entry name" value="P-typ_ATPase_IB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01512">
        <property type="entry name" value="ATPase-IB2_Cd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01525">
        <property type="entry name" value="ATPase-IB_hvy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48085">
        <property type="entry name" value="CADMIUM/ZINC-TRANSPORTING ATPASE HMA2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48085:SF5">
        <property type="entry name" value="CADMIUM_ZINC-TRANSPORTING ATPASE HMA4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="230"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="260"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="568"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="593"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="10">
        <location>
            <begin position="124"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="742"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="816"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="742"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="819"/>
            <end position="833"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7739098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="896" mass="97358" checksum="503695D6C9C4C4E9" modified="2024-05-29" version="1">MKGDQSYRNKWPSPFAVACGVLLVLSFLKYVYSPFKWLALGAVAVGIVPLFLKAIASLRNLRIDINVLMLIAVGGSIFLKDYWEAGTIVFLLTISEWLETRASHKATAVMSSLMSIAPQKAVLAETGEEVNTKDVMVNTRLAVKAGDMIPIDGVVVDGSCEVDEKALTGESFPVSKQVDSIVWAGTVNLNGYISVKTTTLAEACVVAKMAKLVEEAQNNKSKTQRYVDKIANYYTPAVCVVAACLAGIPAALRVHDVEKWYHLALVVLVSACPCALILSTPVAAFCALSKAATSGLLVKGAEYLETLSMVKFICFDKTGTITKGEFSVSSFNPLVDNHKLLYWVSSLESKSSHPMAAALVDYARANSVESQPDNVEEFKDFPGEGIYGKIDGKDVYIGNQKIAIRAGCSQVPMNRSESTEGKSTGYVFVESTLAGTFSLADSCRIGVKEALEQLKSMGIRTAMLTGDCQAAADYAQNQLGGALEVVHAGLLPQDKAKIIKEIQKESKTAMVGDGLNDAPALATADIGISMGVSGSALANETGHVILMSNDIRKIPVAVKLARKARRKIFENIFISVITKVGIIGLAIAGHPLVWAAVLADVGTCLLVIFNSMLLLQGTSTRGVKAHSGSSHNHAQCLSSGNKVQKHGGCCGHDHEDNSLELKHLVQETEDSDSCCGHDDHDHGHGHGHDHHVPESNDECCVHDDHDHGHGHGHDHHVHDTSHCTQETKDECCTHDVNEAKHAVQTEKGKEKEKHECCTHDDDNDEDEIHEVKQPQHEAQTAHKPKQQQTKCCNVTKDLESQSSEKSVNHCHSTANAKKHDNHHHHHHHHHHHEPVKEYVTGEDLGKAMENCCRSHGLKKRHVKGCCKSFISYRCCGTQYFGGGFNRKGYLSEIVIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-10-08" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CC18</accession>
    <name>A0AAD5CC18_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_006851</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008754">
        <property type="protein sequence ID" value="KAI7738674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009630">
        <property type="term" value="P:gravitropism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000554">
        <property type="entry name" value="protein indeterminate-domain 12-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055186">
        <property type="entry name" value="C2H2-2nd_BIRD-IDD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34946">
        <property type="entry name" value="OS03G0310200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34946:SF18">
        <property type="entry name" value="ZINC FINGER C2H2-TYPE_INTEGRASE DNA-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22996">
        <property type="entry name" value="C2H2-2nd_BIRD-IDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="66"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="289"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="11"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7738674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="393" mass="44416" checksum="BF3C6B453E845CDD" modified="2024-05-29" version="1">MLDNHHHHHFASASGPGPGPSSSADLFSSPDNGLPNKRKRRPAGTPDPDAEVVSLSPKTLLESDQYVCEICNQGFQRDQNLQMHRRRHKVPWKLLKRETPEIKKRVFVCPEPSCLHHDPCHALGDLVGIKKHFRRKHSNNKQWVCDKCSKGYAVQSXXXXLILKHVVQEAILVTVVECFPVESFIEHQDACMVRRIHADVPAFPQACSSRTASSSSQSNDMNFGSVQTMSRLPHLQPQSHFSQTQLDIQNNLELQLLPSLSLYEQSNNHQTHLNLSIGSGHSLQQEEEMKIAMADKAFAEDERQQAKRHIEIAEMEFENAKRIRQQAQVELERAKVLREQATKKIESTMLEITCYSCRQRIESIRNNGVAISADETSIAPSYMSSALTEGEGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CCC8</accession>
    <name>A0AAD5CCC8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Nuclear pore complex protein Nup85</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_010003</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Functions as a component of the nuclear pore complex (NPC).</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the nuclear pore complex (NPC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
            <location evidence="1 9">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the nucleoporin Nup85 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008780">
        <property type="protein sequence ID" value="KAI7738590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CCC8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031080">
        <property type="term" value="C:nuclear pore outer ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011502">
        <property type="entry name" value="Nucleoporin_Nup85"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13373">
        <property type="entry name" value="FROUNT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13373:SF21">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07575">
        <property type="entry name" value="Nucleopor_Nup85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 9" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="7 9" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0811">Translocation</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7738590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="718" mass="80957" checksum="590E402F1F36DC18" modified="2024-05-29" version="1">MPGLLTPSTDTSSSIIPYSSDSTTPTVYTLNHNLKPPTTPRLSISWSRGNSLRVTVFRSPATTDNEPGGKVVELNLSSEDVDSDIEMNDAARWRRIVYGSVSPFAHLQNKKNALAALDEFRRSVSHDGDWSKYVLQYSKEINSLLGSKKSSPTPLIEDPKTVLKVEEPSSLKAAWELLEIFYADKQSQPWIPEQLVDWLSDYDALFSGQVPSVHLKLVEFQAELINIQAVETEPAYWQAISSALAVGWLDIVVKLLRLHGSYQLNQLGTRETENGLVEAVAVLVSKMPRMRTDLPLGKLGECYNNKSDFMRAWEKWRAQITKLDCSAFWLQCSHNQTKENLKNLLQILLGNTSTVSSATCHWVELYIAHFLYIRPFTSGLESMYVLAQKCIQLKPISTPHKLMRLILGILEENTEVVLAECSRSFGPWMIAHATELLTAGSVEAEILLKEERDNLGGISIEESHRLVYAQVLSSHALTWQIAPIYLISCMKHGVGLLEILLYKQPAYHSQVLLKNIEICRMYDLDSVSSTAMKIAGVHHWKHGRKGTGVFWLQQARDEVRLNRIAQKLFDFVGKSISDESFEQWEGLIELLGSESKTVGGLEFLHKYRDFKKSLQQVQDGVFTDAARKAGEALILLMKNPSTPQRFWLPLLYDSLKLLNWRERPLLNVSQTNMLLNKLQELSVARLRPDFVEAGLPQEALSSVRVALATNLGRAILEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CCP1</accession>
    <name>A0AAD5CCP1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_009548</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008614">
        <property type="protein sequence ID" value="KAI7739501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CCP1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052266">
        <property type="entry name" value="Miro-EF-hand_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46819">
        <property type="entry name" value="EF-HAND CALCIUM-BINDING DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46819:SF1">
        <property type="entry name" value="EF-HAND CALCIUM-BINDING DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="109"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7739501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="141" mass="15564" checksum="BBF5904CEE5EF2B0" modified="2024-05-29" version="1" fragment="single">SLYYSSDEFSLKGASKLLMEVARKGEDSGYGVPCLLISAKNDLNLYPMAIKDSEMAVKINAPICISAKEKYMNNVFQKIVTTAQKCHLSVPETEHGRNKKRYRQLVNRSLVFASVGAAIAIVALAAYRAYACRKAKSPRQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CCU2</accession>
    <name>A0AAD5CCU2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">ADP-ribosylation factor 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">M8C21_017512</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">AA19_3_7</strain>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008641">
        <property type="protein sequence ID" value="KAI7739282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CCU2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016004">
        <property type="term" value="F:phospholipase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04150">
        <property type="entry name" value="Arf1_5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003500">
        <property type="entry name" value="ADP-ribosylation factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045872">
        <property type="entry name" value="Arf1-5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11711">
        <property type="entry name" value="ADP RIBOSYLATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 12 14" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0519">Myristate</keyword>
    <keyword evidence="5 12 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="24"/>
            <end position="31"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="126"/>
            <end position="129"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00040200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAI7739282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="203" mass="23267" checksum="DBE52F494CB7E548" modified="2024-05-29" version="1">MGMAISRLVKMLFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKDVSFTVWDVGGQDKIRPLWRHYFQNTQGLIFVIDSNDRERITEARDELHRMVNEDALRDATILVFANKQDLPNAMKASEITDKLGLHSLRNRRWYIQSTCATSGEGLYEGLDWLSNNISTKARIRVISHHYNAKTVHLHPSINY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CER5</accession>
    <name>A0AAD5CER5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycosyl transferase family 1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_010332</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008511">
        <property type="protein sequence ID" value="KAI7740114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CER5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46635">
        <property type="entry name" value="GLYCOSYL TRANSFERASE FAMILY 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46635:SF1">
        <property type="entry name" value="GLYCOSYL TRANSFERASE FAMILY 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="4">
        <location>
            <begin position="350"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="937"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="942"/>
            <end position="961"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7740114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="993" mass="113926" checksum="7EF1BD7042F1F930" modified="2024-05-29" version="1">MVFKKLDYLQWVSAVAVFFLFMFVFQMLLPLSTLEQKHINNNNINNLEGDLKSLFQVISGLDFGEGVKFEPTKLLLKFQGDSNNNNVENLLFGGSRKVERFGLRKPQLAFVFADLLVDPQQVLMVSVAAALRAIGYEIEVYSLEDGPVHAVWKNVGVPVNIVEVSGDTKIIIDWLNYDGILVNSLEAKDAISGLLQEPFKSLPLIWTIHEKTLATLHKSYVSDGQFQLINDWKTVFNRATVVVFPNHVLPMYYAAFDAGNYFVIPGSPSEACKLDNSIIVQEESLRVRMNVRAHDFVIAITGSQFLYKGLWVEHALVLRALLPLLAEFPVDDDGLSQRLRIIILSQDLTGNYSAAIEEIALNLKYPRGTVSHAAIDDDGDVYNLLSIADLVIHGSFLEEQSFPDILIKAMCFEKPIIAPDLPIIKKYIDDKVNGYLFPKENIKVLTQIMLQLVSKGKLSSLARNIASTGKHTATNMMVVEAVEGYASLIQNVVNLPSEVASPRAVSEIPSNFKAEWQWRFFEAVADSTYVNKTKRIYQFLNKVEYRWKHNVKESFADISADDTFLYSIWQEERSIQTAIAKKRREDGEVRDRSEQPRGRWEDVYRSAKRAERSKNALHERDDGELERTGQPLCIYEPYFGQGAWPFLHQGSLYRGLGLSTKGRRSRDDIDAPSRLPLLSIPYYRDALGDFGAFFATANRVDRIHKNAWIGFSSWRATAKKAYDIQARRHGDALYFWVRMDKDPRNPMQHDFWTFCDAINAGNCRSAFSEALKKMYGVRGNLTSLPPMPADGDSWSVMHSWSMPTKSFLEFVMFSRMFVDALDAQVYEEHHQSGFCYLSLSKDKQCYSRVLELLVNVWAYHSARRMVYIDPTTGTLEEQHNFKSRRGKMWIKWFSYKTLKTMDEDLAEEADSDHPKRRWLWPSTGEVFWPGMYEKERNQMRQQKQKRKQKSKDRIRRIKNRSQQKALGTYVKPPPDNETDYTSNPVLEATMILR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CF77</accession>
    <name>A0AAD5CF77_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_021982</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM120 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008327">
        <property type="protein sequence ID" value="KAI7740882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CF77"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012926">
        <property type="entry name" value="TMEM120A/B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21433:SF0">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 120 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21433">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN INDUCED BY TUMOR NECROSIS FACTOR ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07851">
        <property type="entry name" value="TMEM120A-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="52"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7740882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="101" mass="11147" checksum="8D4CE6A0B9D6C113" modified="2024-05-29" version="1" fragment="single">ARRMDVVWGETAGVKGQLWLLLPILFVLQAFEAYVGVLLLQTAVSGVVSEWQVVTCGILLIIMAVGNFTNTVQTLVLKSRFKAKMKRGKSKSDLTHVSKDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CF79</accession>
    <name>A0AAD5CF79_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_012234</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008617">
        <property type="protein sequence ID" value="KAI7739489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CF79"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7739489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="318" mass="36218" checksum="74138BC3B1E892A8" modified="2024-05-29" version="1" fragment="single">FKRNRGNLGFNWVIAINSKQSIQTIDLFPQAVAAKDYTVDMATATKDYTVDMAVARRTTRSRRPNKPRTTPILWWRSKLKSLVHFSLNMAINGLKRFVVHVKGIPIGHFCHGPSSPKNEDDILREQIHWVYENCGSVPFSSLEHPKFNAILNKLRLPSVGRMDLAGERLDSGYKQARIRDAMFFQIASDGWKSNRGFESFDSFDSLVNLLVNLPNGTGLIDGRCLQTGNVSELFKVANFINNKSHVKKSLVKYHLQEYGRVGLLRVPINGGNIFVFAFETVFDFVEDVLSSARALQLVVLDEGYKFRRRIKLRGRLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CG12</accession>
    <name>A0AAD5CG12_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Xyloglucan endotransglucosylase/hydrolase</fullName>
            <ecNumber evidence="6">2.4.1.207</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_025513</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Secreted</location>
            <location evidence="6">Cell wall</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Secreted</location>
            <location evidence="6">Extracellular space</location>
            <location evidence="6">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="6">Contains at least one intrachain disulfide bond essential for its enzymatic activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the glycosyl hydrolase 16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.207" evidence="6"/>
    <dbReference type="EMBL" id="JAMZMK010008567">
        <property type="protein sequence ID" value="KAI7739791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CG12"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016762">
        <property type="term" value="F:xyloglucan:xyloglucosyl transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042546">
        <property type="term" value="P:cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010411">
        <property type="term" value="P:xyloglucan metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000757">
        <property type="entry name" value="Beta-glucanase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044791">
        <property type="entry name" value="Beta-glucanase/XTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010713">
        <property type="entry name" value="XET_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016455">
        <property type="entry name" value="XTH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31062">
        <property type="entry name" value="XYLOGLUCAN ENDOTRANSGLUCOSYLASE/HYDROLASE PROTEIN 8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00722">
        <property type="entry name" value="Glyco_hydro_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06955">
        <property type="entry name" value="XET_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005604">
        <property type="entry name" value="XET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51762">
        <property type="entry name" value="GH16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0052">Apoplast</keyword>
    <keyword evidence="6" id="KW-0134">Cell wall</keyword>
    <keyword evidence="6" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="1 6" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041779198" description="Xyloglucan endotransglucosylase/hydrolase" evidence="6">
        <location>
            <begin position="32"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="GH16" evidence="7">
        <location>
            <begin position="37"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="116"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR005604-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7739791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="340" mass="39671" checksum="BBE930A9C440B709" modified="2024-05-29" version="1">MKRPSVHHLLLFTLCVYHMFTGNIHINNVMAFDLNTITFDQGYTPLFSDFNIDRSQDDTRVRLLLNRQSGCGIISTDYYNYGFFSAKIKLPMEKTPGIVVAFYTSNVDMFPKTHDELDVEFLGNARGWTFQTNVYGNGSTTRGREERYRLWFDPSKEFHRYSILWTRNKIIFYVDEIPIREVMRDAGMGGDYPSKPMSSYATVWDASTWATGGGRHKVNYCFEPFTSELQDLVLQGCPVDPIDATSTNCLDAIDELEASEFATITPRQRQAKKWFREKYMYYSYCYDRRRYPSPLPECLLVSSEEELFKTNGRLKNPQQFQQYWHSPGESQRQPEMTAAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CHE2</accession>
    <name>A0AAD5CHE2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_012354</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008079">
        <property type="protein sequence ID" value="KAI7742098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CHE2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7742098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="197" mass="21731" checksum="DA41DCF3FAB35A45" modified="2024-05-29" version="1" fragment="single">VQVFKATTPPSYIYPPSFSFYESFASVSVLRRRPPPPSPTPGCLLLTGYAGRRLLGDAALNPYSMLTILTVVGIRYGFRGDTSEVVGGDGTVVVFMLMNGRNQRKLALDTLINGIVRMPCAFHNDGIHFVAMITMYVYANYVLPGSKDMPGSDEYFDQVVYIQLDITESNKCLDEMKAKLQSGITISPYSSSSPVRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CHV1</accession>
    <name>A0AAD5CHV1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_029946</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008059">
        <property type="protein sequence ID" value="KAI7742187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CHV1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7742187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="114" mass="13573" checksum="719C3136D075EA04" modified="2024-05-29" version="1" fragment="single">AQRLNDLGDESRLLPLWRQASVAEPRFLWNNYMLEVLIDNKQVMFHFDIDQNYCFKGLNVGLRIVGLLYDDISLQNVLDMTVDSTREERQMLRNKDGMERRGYKETWFLNEVAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CIS5</accession>
    <name>A0AAD5CIS5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_011936</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007955">
        <property type="protein sequence ID" value="KAI7742598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CIS5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000051">
        <property type="entry name" value="Receptor-like serine/threonine-protein kinase ALE2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050823">
        <property type="entry name" value="Plant_Ser_Thr_Prot_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45621">
        <property type="entry name" value="OS01G0588500 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="290"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="225"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="557"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="605"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7742598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="605" mass="68764" checksum="5A193F4C354F15DF" modified="2024-05-29" version="1" fragment="single">TEINYFGRLYHPNLVRLIGYCLEDDERLLVYEYMNRASLENHIFNSEGGSYSQTLWWNLRLKVALGAAKGLAYLHCPEVNVIYRDFKSSHILIDWEYNAKLCDFGFAKDGPLDGKSHISTSVMGTYGYAAPEYIATGHLTKRSDIYSFGVVLLEILTGRRSIDNNLTSDEQVLVDFAKPYLTNKIRILHIMDQAIAGQYPSKVAIRAADLAMKCISSEPKSRPIADELVKVLEQLQEMQEGWEKENRRKETDEVKFATITPSPRSQDEILQSTNLKSFSFHVLKAATRNFRPKAVVGVGGFDSVYKAYIDEQSLLAGKPGTGTVVAVKRCENLREWLTEINYLGRLYHPNLVQLIGYCLEDNQRLLVYEYMNQGSLEKHIFRRGSYSQTLRWNLRLKVALGAAKGLAYLHCPEVNVIYRDFKSSCILIDSGYNAKLSDFGLAKDGPLDGKSHVSTRVMGTHGYAAPEYIATGHLSKRSDIYSFGVVLLEILTGRRCFDHDSKQWLVDFAEHYLTSKRSILKIMDRRIAGPYSSKIAIRAADIAMKCISSEPKSRPTADELVKVLEQLQEMQEGWEKENRRKETGTLFKTSWIPPRYAAELLFVTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CJJ5</accession>
    <name>A0AAD5CJJ5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CRAL/TRIO N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_006608</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007819">
        <property type="protein sequence ID" value="KAI7743071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CJJ5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47104">
        <property type="entry name" value="SEC14P-LIKE PHOSPHATIDYLINOSITOL TRANSFER FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47104:SF1">
        <property type="entry name" value="SEC14P-LIKE PHOSPHATIDYLINOSITOL TRANSFER FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46938">
        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7743071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="109" mass="12016" checksum="03E94084955D93DB" modified="2024-05-29" version="1" fragment="single">MVNKHHNSSTSPQIITHIDKNRAQAVLQLVKKASPLTLKQEKFCNEACIGRFLKAKGDNVKKAAKQLRACLSWRENLGVDDLIADDFSGEIADGMAYVAGHDDQSRPVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CLP0</accession>
    <name>A0AAD5CLP0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 20">Pyruvate kinase</fullName>
            <ecNumber evidence="6 20">2.7.1.40</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">M8C21_013831</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="24">AA19_3_7</strain>
            <tissue evidence="24">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19 20">
            <text>pyruvate + ATP = phosphoenolpyruvate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18157"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.40"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 20">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Plastid</location>
            <location evidence="3">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 20">Belongs to the pyruvate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.40" evidence="6 20"/>
    <dbReference type="EMBL" id="JAMZMK010007544">
        <property type="protein sequence ID" value="KAI7744378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009570">
        <property type="term" value="C:chloroplast stroma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030955">
        <property type="term" value="F:potassium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004743">
        <property type="term" value="F:pyruvate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.33.10:FF:000003">
        <property type="entry name" value="Pyruvate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.60:FF:000025">
        <property type="entry name" value="Pyruvate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="entry name" value="Phosphoenolpyruvate-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.33.10">
        <property type="entry name" value="PK beta-barrel domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1380.20">
        <property type="entry name" value="Pyruvate kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001697">
        <property type="entry name" value="Pyr_Knase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011037">
        <property type="entry name" value="Pyrv_Knase-like_insert_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018209">
        <property type="entry name" value="Pyrv_Knase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015793">
        <property type="entry name" value="Pyrv_Knase_brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015795">
        <property type="entry name" value="Pyrv_Knase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036918">
        <property type="entry name" value="Pyrv_Knase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015806">
        <property type="entry name" value="Pyrv_Knase_insert_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004491">
        <property type="entry name" value="PRK05826.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01064">
        <property type="entry name" value="pyruv_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11817">
        <property type="entry name" value="PYRUVATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00224">
        <property type="entry name" value="PK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02887">
        <property type="entry name" value="PK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01050">
        <property type="entry name" value="PYRUVTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50800">
        <property type="entry name" value="PK beta-barrel domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52935">
        <property type="entry name" value="PK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00110">
        <property type="entry name" value="PYRUVATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="17 20" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="12 20" id="KW-0418">Kinase</keyword>
    <keyword evidence="14 20" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0934">Plastid</keyword>
    <keyword evidence="16" id="KW-0630">Potassium</keyword>
    <keyword evidence="18" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 20" id="KW-0808">Transferase</keyword>
    <keyword evidence="15" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Pyruvate kinase barrel" evidence="22">
        <location>
            <begin position="97"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate kinase C-terminal" evidence="23">
        <location>
            <begin position="455"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="78"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="78"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU000504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF00224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF02887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="KAI7744378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="568" mass="62628" checksum="E14A9BA8491703FE" modified="2024-05-29" version="1">MATINLRTPAISPSFKSDTDRRLSSANNLNVSSPSVHEFVYSNPQQRVRKSVSSIAVKSSKIVSQSVEPVLFSPNGPVSLSSDSSLRQPQISPDAQRKTKIVCTIGPSTSSREMIWKLAETGMNVARLNMSHGDHASHKKTVDLVKEYNAQFNEKVIAIMLDTKGPEVRSGDVPKPIFLQEGQEFNFTIERGVSTNDTVSVNYDDFINDVEPGDILLVDGGMMSLEVKSKTKDLVKCEVIDGGELKSRRHLNVRGKSATLPSITDKDWEDIKFGVDNQVDFYAVSFVKNAEVVRELKDYLKSCNADIHVIVKIESADSIVNLPSILAASDGAMVARGDLGAELPIEEVPLLQEDIIRRCQNLQKPVIVATNMLESMIGHPTPTRAEVSDIAIAVRQGADAIMXXXXLPMASKFPLKAVKVMHTVALRTESSSISMVPLARKTAKKSHMGEIFGYHAATIANTLSTPIIVFTRTGSMAVILSHYRPFSTIFAFTNVKRVQQRLMLYHGVMPIYMEFSDDAEETFSRALNIIVGKSLVKEGQYVTLVQSGAQPIWRKESTHHIQVRMVQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CMC4</accession>
    <name>A0AAD5CMC4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">FAS1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_018112</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">May be a cell surface adhesion protein.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the fasciclin-like AGP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007553">
        <property type="protein sequence ID" value="KAI7744277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CMC4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.180.10:FF:000011">
        <property type="entry name" value="Fasciclin-like arabinogalactan protein 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.180.10">
        <property type="entry name" value="FAS1 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036378">
        <property type="entry name" value="FAS1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000782">
        <property type="entry name" value="FAS1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044654">
        <property type="entry name" value="FLA15/16/17/18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32499:SF13">
        <property type="entry name" value="FAS1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32499">
        <property type="entry name" value="FASCICLIN-LIKE ARABINOGALACTAN PROTEIN 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02469">
        <property type="entry name" value="Fasciclin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00554">
        <property type="entry name" value="FAS1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82153">
        <property type="entry name" value="FAS1 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50213">
        <property type="entry name" value="FAS1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0654">Proteoglycan</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042212350" description="FAS1 domain-containing protein" evidence="10">
        <location>
            <begin position="26"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="domain" description="FAS1" evidence="11">
        <location>
            <begin position="32"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="FAS1" evidence="11">
        <location>
            <begin position="261"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="206"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="218"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="239"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7744277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="448" mass="48923" checksum="4E4684C1CCB54FBA" modified="2024-05-29" version="1">MDSKIYGASNLVILMLSISFINVNALQSTQVSSNSVLVALLDSHYTELSELVEKALLLQTLEQAVSNHNITIFAPNNEALERQMDPEFKRFLLEPRNLKSLQKLILYHIIPGRVGSHNWPREHITHHKTLCVGESDNRLPLTKGNKGEKIVGGVAKIVRADDVIRPDGLIHGIERLLVPQSVQEDFNRRRSLGSISAVLPEGAPVVDPRTHRLKKPAGPAPAGSPPALPVYDAMAPGPDLAPAPAPGPGGPRHHFDGESQVKDFIQTLLHYGGYNELADILVNLTSLATEMGKLVSEGYVLTVLAPNDEAMAKLTTDQLTDQGAPEQIMYYHLIPEYQTEESMYNSVRRFGRVSYDTLRLPHKVVAEEADGSVKFGQRDESAYLIDPDIYTDGRISVQGIDGVLFPVVKTAETTVAPSASVAKVVTKQRRGKLMEMGCTIAGALGHSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CN13</accession>
    <name>A0AAD5CN13_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">3-hydroxyacyl-CoA dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">M8C21_016902</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">AA19_3_7</strain>
            <tissue evidence="21">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>(3S)-3-hydroxybutanoyl-CoA = (3R)-3-hydroxybutanoyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:21760"/>
            <dbReference type="ChEBI" id="CHEBI:57315"/>
            <dbReference type="ChEBI" id="CHEBI:57316"/>
            <dbReference type="EC" id="5.1.2.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a (3E)-enoyl-CoA = a 4-saturated (2E)-enoyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:45228"/>
            <dbReference type="ChEBI" id="CHEBI:58521"/>
            <dbReference type="ChEBI" id="CHEBI:85097"/>
            <dbReference type="EC" id="5.3.3.8"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a (3S)-3-hydroxyacyl-CoA = a (2E)-enoyl-CoA + H2O</text>
            <dbReference type="Rhea" id="RHEA:16105"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57318"/>
            <dbReference type="ChEBI" id="CHEBI:58856"/>
            <dbReference type="EC" id="4.2.1.17"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a (3Z)-enoyl-CoA = a 4-saturated (2E)-enoyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:45900"/>
            <dbReference type="ChEBI" id="CHEBI:85097"/>
            <dbReference type="ChEBI" id="CHEBI:85489"/>
            <dbReference type="EC" id="5.3.3.8"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a 4-saturated-(3S)-3-hydroxyacyl-CoA = a (3E)-enoyl-CoA + H2O</text>
            <dbReference type="Rhea" id="RHEA:20724"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58521"/>
            <dbReference type="ChEBI" id="CHEBI:137480"/>
            <dbReference type="EC" id="4.2.1.17"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Lipid metabolism; fatty acid beta-oxidation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">In the central section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007447">
        <property type="protein sequence ID" value="KAI7744767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CN13"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003857">
        <property type="term" value="F:(3S)-3-hydroxyacyl-CoA dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008692">
        <property type="term" value="F:3-hydroxybutyryl-CoA epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004165">
        <property type="term" value="F:delta(3)-delta(2)-enoyl-CoA isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004300">
        <property type="term" value="F:enoyl-CoA hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000009">
        <property type="entry name" value="Fatty oxidation complex, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1040.50:FF:000004">
        <property type="entry name" value="Peroxisomal fatty acid beta-oxidation multifunctional protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000025">
        <property type="entry name" value="Peroxisomal fatty acid beta-oxidation multifunctional protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006180">
        <property type="entry name" value="3-OHacyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006176">
        <property type="entry name" value="3-OHacyl-CoA_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006108">
        <property type="entry name" value="3HC_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018376">
        <property type="entry name" value="Enoyl-CoA_hyd/isom_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23309">
        <property type="entry name" value="3-HYDROXYACYL-COA DEHYROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23309:SF9">
        <property type="entry name" value="PEROXISOMAL FATTY ACID BETA-OXIDATION MULTIFUNCTIONAL PROTEIN MFP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00725">
        <property type="entry name" value="3HCDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02737">
        <property type="entry name" value="3HCDH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00067">
        <property type="entry name" value="3HCDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00166">
        <property type="entry name" value="ENOYL_COA_HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="12" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13" id="KW-0456">Lyase</keyword>
    <keyword evidence="14" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="9" id="KW-0520">NAD</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3-hydroxyacyl-CoA dehydrogenase NAD binding" evidence="20">
        <location>
            <begin position="314"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="domain" description="3-hydroxyacyl-CoA dehydrogenase C-terminal" evidence="19">
        <location>
            <begin position="495"/>
            <end position="588"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00008750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU003707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF02737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAI7744767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="23">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="725" mass="79061" checksum="8D510613B13D91D1" modified="2024-05-29" version="1">MGGTTTISVGSDGVAIITIINPPVNSLSFDVLNSLKERFDQALQRDDVKAIVVTGAKGKFSGGFDISAFGALQEGKAMTTGLASKPGYVSIDILSDTVEAAKKPSVAAIDGLALGGGLEVAMACHARIATSTAQLGLPELQLGIIPGFGGTQRLPRLVGLAKALEMMLTSKPVRGEEAHDLGLVDTIVSGDELLEKARQMALDILARRKPWISSLYRTDKIEPLGEAREILNFARAQARRQAPNLQHPQVCIDVIEEGVVSGPRAGLLKEYESFQVLLKSDTCKSLIHVFFAQRGTTKVPGVTDLGLKPRQIKKVAVLGGGLMGSGIATALVLNGYEVVLKEVNQKFLDAGLGRVKANLMSSVKKGRLTQEKFEKTMSRLKGVLDYESFKYVDMVIEAVIENVSLKQQIFLDLEKYCSPNCILASNTSTIDLNLIGEKTKSHDRIIGAHFFSPAHVMPLLEIVRTPKTSPQTVVDLLDVGKKIRKTPVVVGNCTGFAVNRMFFPYTQAALLLVERGADIYKIDRAITKFGMPMGPFRLCDLVGFGVAVATGSQFVFNFPERTYKSMLIPLMQEDKRAGETTRKGFYLYNDKRKASPDPEITKYIEKAREISGISVEPKFGKLSDKDIIEMVFFPVVNEACRVYAEGIAVKAADLDIAGVMGMGFPPYRGGIMFWGDSIGSKYIYERLNEWSKMYGEFFKPCDYLAQRATLGAPLSSPLDQGKSRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CNA7</accession>
    <name>A0AAD5CNA7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NADH:flavin oxidoreductase/NADH oxidase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_020192</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NADH:flavin oxidoreductase/NADH oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007415">
        <property type="protein sequence ID" value="KAI7744917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CNA7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001155">
        <property type="entry name" value="OxRdtase_FMN_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045247">
        <property type="entry name" value="Oye-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22893:SF114">
        <property type="entry name" value="12-OXOPHYTODIENOATE REDUCTASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22893">
        <property type="entry name" value="NADH OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00724">
        <property type="entry name" value="Oxidored_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0288">FMN</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADH:flavin oxidoreductase/NADH oxidase N-terminal" evidence="6">
        <location>
            <begin position="44"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7744917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="264" mass="29199" checksum="016BA607337DB300" modified="2024-05-29" version="1">MLLFIPFFSYVITKLGFQPNGQAPVSSTDKGVTPGLDGVDWSPPRRLRTDEIPALINDFRLAARNAIDAGFDGVEIHGANGYIIDQFLKDQVNDRTDQYGGTLENRCRLALEIVEAVANEIGGDKVGIRLSTFADYMECGDSNPEALGLHMANELNKYGILYLHVIEPRMVQVGNKAETPYSTLPMKKAFKGTFISAGGYKRDDGDKAVADGNADLVAFGRLFLANPDLPRRFEVDADLNKYNRATFYIQDPVVGYTDYPFLEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CPM7</accession>
    <name>A0AAD5CPM7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">phenylalanine--tRNA ligase</fullName>
            <ecNumber evidence="3">6.1.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Phenylalanyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">M8C21_007282</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">AA19_3_7</strain>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Is responsible for the charging of tRNA(Phe) with phenylalanine in mitochondrial translation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>tRNA(Phe) + L-phenylalanine + ATP = L-phenylalanyl-tRNA(Phe) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19413"/>
            <dbReference type="Rhea" id="RHEA-COMP:9668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78531"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.20"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.20" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010007093">
        <property type="protein sequence ID" value="KAI7745861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CPM7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004826">
        <property type="term" value="F:phenylalanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006432">
        <property type="term" value="P:phenylalanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00496">
        <property type="entry name" value="PheRS_alpha_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.380:FF:000003">
        <property type="entry name" value="Phenylalanine--tRNA ligase chloroplastic/mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000082">
        <property type="entry name" value="Phenylalanine--tRNA ligase chloroplastic/mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000080">
        <property type="entry name" value="phenylalanine--tRNA ligase, chloroplastic/mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.380">
        <property type="entry name" value="Ferrodoxin-fold anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005121">
        <property type="entry name" value="Fdx_antiC-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036690">
        <property type="entry name" value="Fdx_antiC-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004530">
        <property type="entry name" value="Phe-tRNA-synth_IIc_mito"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002319">
        <property type="entry name" value="Phenylalanyl-tRNA_Synthase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00469">
        <property type="entry name" value="pheS_mito"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11538:SF41">
        <property type="entry name" value="PHENYLALANINE--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11538">
        <property type="entry name" value="PHENYLALANYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03147">
        <property type="entry name" value="FDX-ACB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01409">
        <property type="entry name" value="tRNA-synt_2d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00896">
        <property type="entry name" value="FDX-ACB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54991">
        <property type="entry name" value="Anticodon-binding domain of PheRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51447">
        <property type="entry name" value="FDX_ACB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="14">
        <location>
            <begin position="180"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="FDX-ACB" evidence="15">
        <location>
            <begin position="337"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00057761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAI7745861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="427" mass="49257" checksum="58C665E776F1D6DC" modified="2024-05-29" version="1">MAALSFAHHILLSKPSFFLHSSKTLTKTNTFFSISISSPFIRRHKSTQTIASSLLELGGVQISKQDVVKDEPTNNVPDLIFTKLGLQLHRRDNHPLGILKNAIYEYFDSNYNNQFLKFDDLCPIVTTKQNFDDVLVPADHVSRSYNDTYYVDAETVLRCHTSAHQAELLRKGYTNFLVTGDVYRRDSIDSTHYPVFHQMEGVRVFSPNDWKDSGMDGTSYAADDLKACLEGLARHLFGAVEMRWVDTYFPFTNPSFELEIYFQEKWLEVLGCGVMEQEILKRGGKTDNVAWAFGLGLERLAMVLFDIPDIRLFWSTDKRFTSQFTKGQLGIKFKPFSKYPPCYKDMSFWINDSFTENNLCEIVRGVAGDLAEEVQLIDNFTNKKGMTSHCYRITYRSMERSLTDEEINELQWNVRDQVQSQMNVELR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CQP3</accession>
    <name>A0AAD5CQP3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Remorin C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_000084</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the remorin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007070">
        <property type="protein sequence ID" value="KAI7745972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CQP3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005516">
        <property type="entry name" value="Remorin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31775">
        <property type="entry name" value="OS02G0117200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31775:SF45">
        <property type="entry name" value="REMORIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03763">
        <property type="entry name" value="Remorin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Remorin C-terminal" evidence="4">
        <location>
            <begin position="61"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="96"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="15"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="27"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7745972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="173" mass="19218" checksum="B18079805E425175" modified="2024-05-29" version="1">MGEEQHESNKDIALETESLSTLISTSPPEPKSSPVTTPPPEKENTPQSGDNAGLEIVVTEKRMALIKAWEENEKTRADNKAYTKISAIGAWENTKRAAIEADLKQIEEDIENEKAKQREKMKNKMAAIHKEAEEKRAAIIAKKGQDLIKVEEAAAKFHATGTLPSRLFKCFGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CSK3</accession>
    <name>A0AAD5CSK3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">CASP-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_026243</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3 8">Homodimer and heterodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Casparian strip membrane proteins (CASP) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006958">
        <property type="protein sequence ID" value="KAI7746505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CSK3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006459">
        <property type="entry name" value="CASP/CASPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006702">
        <property type="entry name" value="CASP_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01569">
        <property type="entry name" value="A_tha_TIGR01569"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33573:SF64">
        <property type="entry name" value="CASP-LIKE PROTEIN 2B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33573">
        <property type="entry name" value="CASP-LIKE PROTEIN 4A4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04535">
        <property type="entry name" value="CASP_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="32"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="72"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="109"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="169"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="Casparian strip membrane protein" evidence="9">
        <location>
            <begin position="25"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7746505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="196" mass="21322" checksum="31FBB64B1BD197F0" modified="2024-05-29" version="1">MSYLGVGVSPGKHHVRESDLKALDRKVKIAELILRAAVCGLALIAALLVATDTQVKEIFTIRKRAKFTDMKSMVMLVVVNGIVAAYSLVHTFRCVVSMLRGSVLFNKPLAWLIFSGDQVMAYMIVVAIGASAQSAAYAKLGEPQVEWMKVCDMYAKFCNQVGEGIASSVFACASMIIVSAISAFSLFRLYDGNKAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CWW9</accession>
    <name>A0AAD5CWW9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Peroxisome biogenesis protein 12</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Peroxin-12</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">M8C21_001643</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">AA19_3_7</strain>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Component of a retrotranslocation channel required for peroxisome organization by mediating export of the PEX5 receptor from peroxisomes to the cytosol, thereby promoting PEX5 recycling. The retrotranslocation channel is composed of PEX2, PEX10 and PEX12; each subunit contributing transmembrane segments that coassemble into an open channel that specifically allows the passage of PEX5 through the peroxisomal membrane. PEX12 also regulates PEX5 recycling by activating the E3 ubiquitin-protein ligase activity of PEX10. When PEX5 recycling is compromised, PEX12 stimulates PEX10-mediated polyubiquitination of PEX5, leading to its subsequent degradation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the pex2/pex10/pex12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006372">
        <property type="protein sequence ID" value="KAI7749399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CWW9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990429">
        <property type="term" value="C:peroxisomal importomer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016558">
        <property type="term" value="P:protein import into peroxisome matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006513">
        <property type="term" value="P:protein monoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16451">
        <property type="entry name" value="mRING_PEX12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000357">
        <property type="entry name" value="Peroxisome biogenesis protein 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017375">
        <property type="entry name" value="PEX12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006845">
        <property type="entry name" value="Pex_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12888:SF0">
        <property type="entry name" value="PEROXISOME ASSEMBLY PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12888">
        <property type="entry name" value="PEROXISOME ASSEMBLY PROTEIN 12 PEROXIN-12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04757">
        <property type="entry name" value="Pex2_Pex12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038074">
        <property type="entry name" value="Peroxisome_assembly_p12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13 15" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Pex N-terminal" evidence="16">
        <location>
            <begin position="20"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00045862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR038074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF04757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAI7749399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="364" mass="40740" checksum="51ADAEC3C19E36F3" modified="2024-05-29" version="1">MLFQVGGQGSHPTFFEMSAAQQLPSSLRAALTYSIGVLALRRPFLHKVLDYEDEFFAALTLILETHSLRTTDASFAETLYGLRRRAVNVQVKKDDLHMEAGNRIQHTGLRKRQKLLSVVFLVVLPYFKSKLHAIYNREREAALQASLWGNVDERFDEIDNLGDASSSFASTSGLDTDLSVRTRFRKRMDKIVATCYPWLHAGTEGLAFAYQLLYLLDATGFYSPGLHALGLQVCRATGQELMDTSSRISKIRSNERERLRGPPWLKAVQGALLTCAYAVLDYAQTGLIAAVFGFKVAKEGIPLPPDRTLCPLCSQKRANPSAVTVSGFVFCYACIFKYVSQYNRCPVTLVPANVDQIRRLFHDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CYI4</accession>
    <name>A0AAD5CYI4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">SURP motif domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_005835</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006168">
        <property type="protein sequence ID" value="KAI7750314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CYI4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000395">
        <property type="term" value="P:mRNA 5'-splice site recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.790">
        <property type="entry name" value="Surp module"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000061">
        <property type="entry name" value="Surp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040397">
        <property type="entry name" value="SWAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035967">
        <property type="entry name" value="SWAP/Surp_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019147">
        <property type="entry name" value="SWAP_N_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13161">
        <property type="entry name" value="SPLICING FACTOR SUPPRESSOR OF WHITE APRICOT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13161:SF15">
        <property type="entry name" value="SPLICING FACTOR, SUPPRESSOR OF WHITE-APRICOT HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09750">
        <property type="entry name" value="DRY_EERY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01805">
        <property type="entry name" value="Surp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01141">
        <property type="entry name" value="DRY_EERY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00648">
        <property type="entry name" value="SWAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109905">
        <property type="entry name" value="Surp module (SWAP domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50128">
        <property type="entry name" value="SURP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="SURP motif" evidence="8">
        <location>
            <begin position="147"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="SURP motif" evidence="8">
        <location>
            <begin position="336"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="45"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="224"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="403"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="443"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="550"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="57"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="110"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="236"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="443"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="568"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="599"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="613"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="628"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="647"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="659"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="706"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="727"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="739"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="777"/>
            <end position="793"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7750314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="857" mass="96983" checksum="5F60CD1B22A896FF" modified="2024-05-29" version="1">MNIEIHGRHALLFDDDTAAAFVNSDDALVEWNSLLIDRYDVRHLLSTPPPSRRRRNPQPSHSSSADADLDHERYLDLPSPSDEEKEDEKPEPVGGFNAVPFSYGNDEDSNDQKAAETEPEVPKFHPPFSVPDSLAQNLPPTEKVHQIIARTALFVSKNGGQSEIILRVKQGDNPTFGFLMPDHHLHPYFRFLVENQDLLHSKDNGQTENAEKISNTGALSLLGSVYGTGEDDDGGTEQPSSLSSSKESVSGNSVKVDATDSLTAVKTVFPLGKDEPIIKRNSLSNALKVGRTSSIKKDEFLGSLSSVPDKSRASSLPPIPKTELMLVDPPTDMKRLVEKIVEFIIKNGRQFEAVLMEQDRGHGRFPFLIPSNIFHPYYLKVLQKAQESKLTVKSFYNEKEDFGGHISNKKKSTSRENGNDTVEPDDIPLDSERKEKFKMVINKSKKEGSDLPDKSTQSPPRVQVDADQAAAILQAATRGIRPSTLGILYGQPSNKDISSESQLTHEQKLKAERLKRAKMFVAQLKSGAAPIKAEPSRSLSAEPQVDNRLAANETEKSIPPSDVNTSDNKLKVEKEHLNEEKRAMRKYRARSIIDKKSDEDDNDDDDDDERDRKRSHRHHTKKHRSHNHSGEDEREENCVDERDYKHDKRRHRSHRSSHKHDDMDKISEEKDQRYSKKKHRSSSSYEDDEQDSEHEYSRKKHRTHHSSHDEVDSDNKYSRKKHRTHHSSHDEVDSDDTHSRRKRKHRSHKSSHRHKHSKSHSSRHKEKLDVSSEEESRDQSKTDKNVKGPNSEREELEEGEITSSSKGVHLVDGVSREPSVDVSSSFPDSRPPSQPSGATEVSDDLRAKIRAMLMSTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CZG8</accession>
    <name>A0AAD5CZG8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_006925</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006177">
        <property type="protein sequence ID" value="KAI7750247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CZG8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000342">
        <property type="entry name" value="Protein kinase superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47989">
        <property type="entry name" value="OS01G0750732 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47989:SF27">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041985483" description="Protein kinase domain-containing protein" evidence="9">
        <location>
            <begin position="19"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="66"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="157"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7750247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="377" mass="41117" checksum="5EA1D689C7694054" modified="2024-05-29" version="1" fragment="single">MNLFLGFLLFLNLGFVIASIDMVSSISAPESSPMAAAPFAFVPSSMADFTSGPNVGNHRMKASRKLLIGLVTSLLVIMIIIMSIIVLMKCHRRKLHKLKNPGGKRSGQQMKIIADSLRGLALSSFVGKSNGSLKTVNEKASVAVMDYHLLESATGNFGEGEVLGEGGFGCVYRARIDESLCVAVKRLDGGSQDAIKEFQTEIDLLSKIHHPNIITLLGYCVHDDTRLLVYELMHNGSLETQLHVGLGLFSILGRPFAKTNSRRPSNGSNLTWHCRMKIALDTARGLEYLHENCKPSVIHRDLKSSNILLDSSFNAKVGTLDITIICTTLSYLSQSHNNNASHTYFQNFGLAVMDGAQNKSNVKLSGTLGYVAPEYLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D102</accession>
    <name>A0AAD5D102_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_007016</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006217">
        <property type="protein sequence ID" value="KAI7750095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D102"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37206">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37206:SF4">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="119"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="24"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="151"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="29"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="50"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7750095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="203" mass="22984" checksum="222FE7AAA9E40D84" modified="2024-05-29" version="1">MEDNDWETIQPSSSDEDDAYTVVMTTPTNNNNNNNNNNNNNNNNNNNNNHPSILLQQQDNHLQEPVQESPPPSSHSSSATPYTGDVVESELSQSPEVEVKNTLLKTSLGVLSSWVRSRIGFWSIASVGVFAVVAYARRWHRLKRLAEKDYKDKMLLLLNQKDEKIKQLLRQIDRMNDALSARRRVPVIRVLVDGPVVIGPRLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D1H9</accession>
    <name>A0AAD5D1H9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_003139</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006180">
        <property type="protein sequence ID" value="KAI7750245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D1H9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7750245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="36" mass="4006" checksum="F228229807DEF9E1" modified="2024-05-29" version="1">MIWHTRSRVPQEGVTILVARGTFSKCLGQCEAIGLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D272</accession>
    <name>A0AAD5D272_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">WRKY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_004348</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the WRKY group III family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005838">
        <property type="protein sequence ID" value="KAI7751710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D272"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010150">
        <property type="term" value="P:leaf senescence"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042542">
        <property type="term" value="P:response to hydrogen peroxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010193">
        <property type="term" value="P:response to ozone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009751">
        <property type="term" value="P:response to salicylic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.80:FF:000009">
        <property type="entry name" value="WRKY transcription factor 53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.80">
        <property type="entry name" value="WRKY domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003657">
        <property type="entry name" value="WRKY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036576">
        <property type="entry name" value="WRKY_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044810">
        <property type="entry name" value="WRKY_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32096:SF99">
        <property type="entry name" value="WRKY FAMILY TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32096">
        <property type="entry name" value="WRKY TRANSCRIPTION FACTOR 30-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03106">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00774">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118290">
        <property type="entry name" value="WRKY DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50811">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="WRKY" evidence="8">
        <location>
            <begin position="121"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="66"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="182"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="66"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="198"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00060850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7751710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="322" mass="36308" checksum="373C0706B8521420" modified="2024-05-29" version="1">MDTSVYIINELTQGMELAMHLKSILSSESSPETKQILIQEIISSYDRAMLLINLGDSGAQTATLAPSMLSQPESSVSIDETPRSQDFNQSFDNQQEEKVFSKKRKTMPTWKDQIRISTDSGLEGNTDDGHSWRKYGQKTILGAKFPRSYYRCTYRHAQNCMARKQVQRTDENPTVFDITYRGEHTCNPSAKPSAQRPKSPEKHEIKENNHQRLLPPKSEDMLANLKENLRVNTSDLDTTLPCSFSFPSASFGTFENYQQFNYLQGSPSGSNYFTEWGNDFQYYDDSNLSGMISTTGSATNSPLAFSADPKDLSPFNNSGFLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D2E9</accession>
    <name>A0AAD5D2E9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA-directed DNA polymerase</fullName>
            <ecNumber evidence="2">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_003650</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010005803">
        <property type="protein sequence ID" value="KAI7751830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D2E9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043625">
        <property type="term" value="C:delta DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008296">
        <property type="term" value="F:3'-5'-DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006287">
        <property type="term" value="P:base-excision repair, gap-filling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045004">
        <property type="term" value="P:DNA replication proofreading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006297">
        <property type="term" value="P:nucleotide-excision repair, DNA gap filling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.132.60:FF:000001">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.60">
        <property type="entry name" value="DNA polymerase family B, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1600.10">
        <property type="entry name" value="Palm domain of DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017964">
        <property type="entry name" value="DNA-dir_DNA_pol_B_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006134">
        <property type="entry name" value="DNA-dir_DNA_pol_B_multi_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042087">
        <property type="entry name" value="DNA_pol_B_thumb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023211">
        <property type="entry name" value="DNA_pol_palm_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050240">
        <property type="entry name" value="DNA_pol_type-B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025687">
        <property type="entry name" value="Znf-C4pol"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10322">
        <property type="entry name" value="DNA POLYMERASE CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10322:SF23">
        <property type="entry name" value="DNA POLYMERASE DELTA CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00136">
        <property type="entry name" value="DNA_pol_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14260">
        <property type="entry name" value="zf-C4pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00116">
        <property type="entry name" value="DNA_POLYMERASE_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DNA-directed DNA polymerase family B multifunctional" evidence="11">
        <location>
            <begin position="1"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="C4-type zinc-finger of DNA polymerase delta" evidence="12">
        <location>
            <begin position="283"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF14260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7751830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="373" mass="42584" checksum="AE12751EF60F0A78" modified="2024-05-29" version="1">MIEHTKKLVEDKFTTLNGYEHNAEVIYGDTDSVMVQFGVLTVDEAMKLGREAADYISGTFIKPIKLEFEKVYFPYLLISKKRYAGLYWTNPDKFDKMDTKGIETVRRDNCLLVKNLVTECLNKILIDRDIPGAVQYVKNTISDLLMNRMDLSLLVITKGLTKTGDDYEVKAAHVELAERMRKRDAATAPNVGDRVPYVIIKAAKGAKAYERSEDPIYVLENNIPIDPQYYLENQISKPLLRIFDPILKNASRELLHGSHTRAISISTPSNSGIMRFAKKQLSCVGCKALISNKDQTLCSHCKGREAELYCKTVDNVSELEKLFGRLWTQCQECQGSLHQDVLCTSRDCPIFYRRKKAQKDMAEAKVQLDRWNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D386</accession>
    <name>A0AAD5D386_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein LURP-one-related 17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_002636</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LOR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005794">
        <property type="protein sequence ID" value="KAI7751909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D386"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.200">
        <property type="entry name" value="LURP1-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007612">
        <property type="entry name" value="LOR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038595">
        <property type="entry name" value="LOR_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025659">
        <property type="entry name" value="Tubby-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31087">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31087:SF14">
        <property type="entry name" value="PROTEIN LURP-ONE-RELATED 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04525">
        <property type="entry name" value="LOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54518">
        <property type="entry name" value="Tubby C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="14"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="23"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7751909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="215" mass="24609" checksum="95ACFC6701851B74" modified="2024-05-29" version="1">MHMLRFLKSLSRTVHEERGHEPSNSSDDGCSSVDGGEASSLTVWRKSLFIGCSGFTVINSVGDLVYRVDNYGSRRRQELVLMDASGKPILTVSRSKKLKMVNNWLVFEGELSSKEQKPICRATKYFYVLWPKKRLARVYKEPYDEKNMYMIEGSYMNRSCKVLDESRNVVVEIRRKETVTKGASFGHEVFDLIINRGFDSGLAMAIILLLDQMFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D3C7</accession>
    <name>A0AAD5D3C7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_002871</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005932">
        <property type="protein sequence ID" value="KAI7751311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D3C7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7751311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="76" mass="8042" checksum="D3D45CF20E5C50A0" modified="2024-05-29" version="1">MAPGGNVYEDVTESCADYGVKSSHRSCGSAGGRVGTVSNSGVHDLLKCPMRVTVMCPPIHQRQVSNPKSLLAANRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D4P6</accession>
    <name>A0AAD5D4P6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_005049</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005864">
        <property type="protein sequence ID" value="KAI7751640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D4P6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7751640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="51" mass="5872" checksum="D7C19A156BB7E16A" modified="2024-05-29" version="1" fragment="single">MMYEPGTSGIEMLRRSKDDDKETWGFLKVMFEDDGTSRIKLLNHLALNLPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D4R7</accession>
    <name>A0AAD5D4R7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">HMA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_023577</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the HIPP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005175">
        <property type="protein sequence ID" value="KAI7753903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D4R7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009626">
        <property type="term" value="P:plant-type hypersensitive response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45868:SF57">
        <property type="entry name" value="HEAVY METAL-ASSOCIATED DOMAIN, HMA, HEAVY METAL-ASSOCIATED DOMAIN SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45868">
        <property type="entry name" value="HEAVY METAL-ASSOCIATED ISOPRENYLATED PLANT PROTEIN 33-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0488">Methylation</keyword>
    <keyword evidence="4" id="KW-0636">Prenylation</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMA" evidence="7">
        <location>
            <begin position="21"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="99"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="122"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="150"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="162"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="187"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7753903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="299" mass="32232" checksum="142191FC65EF913A" modified="2024-05-29" version="1">MAKPPVSGNNNTSEHLQSIGLQTWILKVYIHCEGCKKKVYKVLQSIDGRVYKTVIDAKEHKVTVTGSVNGEVLVQKLLKSGKHAEILPESFAATAVADNASGNSKKKKKKQGNKQNDVVTTVDEDELVNDEQNESKEGGNVEIADPAITDGGGNLNGVGGGGKKKKNKKKKGGNNASPNGDTEGEPTLNSPTETGVTAENATASMEQLNLRPQVPQMVYSTPYDLPPYQNYYPTPAYGVSYSTSYPSAESSYYTPPVYGYAQSHPSVFYPVPPPPSSYRRSAFDDYDDEGGEGRVCAIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D610</accession>
    <name>A0AAD5D610_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_013457</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010003049">
        <property type="protein sequence ID" value="KAI7754563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D610"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050823">
        <property type="entry name" value="Plant_Ser_Thr_Prot_Kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45621">
        <property type="entry name" value="OS01G0588500 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7754563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="230" mass="26294" checksum="AF273BE6823924B7" modified="2024-05-29" version="1">MPNGNFEDHLFSGAIARLPLATKVKIAVGIARGIVFLHNSRDYVSTFSQFGGTSMFRLDRHKILLDEDFTAKLSDCELTKLAHGHYPYNIRDDNGLVYGDYYPRFKPIQLQSNLDGFTLVLMEVLTGKQISYDNEVQRMDDLLLQRGKMSIRNIAKLCFEKCNEVDSESTMLTLLEEYEMYIYEAFATATAERGGSHAEYNFNKNWISNEGDGCVGEMELLYDKENHPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D7N0</accession>
    <name>A0AAD5D7N0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">PGG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_021425</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005380">
        <property type="protein sequence ID" value="KAI7753591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D7N0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000217">
        <property type="entry name" value="Ankyrin repeat-containing protein ITN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026961">
        <property type="entry name" value="PGG_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24186:SF48">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN ITN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24186">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13962">
        <property type="entry name" value="PGG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="329"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="369"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="410"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="436"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="105"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="173"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="207"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="241"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="PGG" evidence="9">
        <location>
            <begin position="325"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7753591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="475" mass="52179" checksum="FA3B9B13A43383D0" modified="2024-05-29" version="1">MSSTVDEVDLERGIFMTPSMQNYNSKLLDPSPALTASGKIKYVKHVIGRYNDTQLHLAAYRGDLASVKRILDEINSQMVGTLSGADCDAEVAKIRAAMVNEVNELGETALYIAAEKGHLEVVKELLKYSDKDTLTTSHLDFDPLHIAASQGHTAVVEELLSKDSSLLYIVKSKGKNALHLAARSGHVETVKSLLEKDPQLGRRTDKKGQTALHMAAKGANSGVVKLLLEADAAIVMLPDNSGLTALHVATRKKRLEIVNELLCLPDMNVNALNRDHKTALDVAEELSSSEKSTEIRAALVCHGAFRANELEQPRDELRKLHRKEIKNDGNYVTIAVLFAGIAYAAIFTVPGGNNDNGMAVVVNKTSFKIFFIFNAFALYTSLATVVMHVTLGITDETDEKRRLVVVINKLMWLASVCTSVAYMASTYIVIGRKHEWVAIYTTVVGASIMMLALGVMTHYVHKWRITKNQINDKGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DAC1</accession>
    <name>A0AAD5DAC1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">methionine S-methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_015538</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-methionine + S-adenosyl-L-methionine = S-methyl-L-methionine + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:13761"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:58252"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="2.1.1.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class I-like SAM-binding methyltransferase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.12" evidence="1"/>
    <dbReference type="EMBL" id="JAMZMK010000702">
        <property type="protein sequence ID" value="KAI7755917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030732">
        <property type="term" value="F:methionine S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025779">
        <property type="entry name" value="Met_S-MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47087">
        <property type="entry name" value="METHIONINE S-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47087:SF1">
        <property type="entry name" value="METHIONINE S-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51555">
        <property type="entry name" value="SAM_MT12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="2">
        <location>
            <begin position="770"/>
            <end position="892"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7755917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="1073" mass="119750" checksum="0E18F0CD2BB777CE" modified="2024-05-29" version="1">MGAVNGSIEEFLKQCSESGDSAYSALRSVLERLENPESRAEARVFLAHLQRKVEEDQHCLETYHFQIQDIYLHPDQGFQNRKKLTMMVIPSIFMPEDWSFTFFEGLNRHPDSIFKDKTVAELGCGNGXXXXAIADKWLPFKVYGLDINPRAVKISWINLYLNAFDDNGLPVYDHENKTLLDRVEFYESDLLSYCRDNHIELERIVGCIPQILNPNPDAMSKIITENASEEFLHSLSNYCALQGFVEDQFGLGLIARAVEEGIDVIKPMGIMIFNMGGRPGQGVCKRLFERRGLRVNKLWQTKILQASDTDISALVEIEKNNPHRFEFFMGLVGDQPICARTAWAFGKAGGRISHALSVYSCQLRHPNQVKKIFEFLKNGFHDISNSLDLSFEDDSVADEKIPFLAYLAGVLNDRSRFPYEPPIGSKRFRDLIAGFMRTYHHVPLSADNVAIFPSRATAIENALQLFTPRLAIVDEHLTRHLPRQWLTSLEIEHIKDNKTATDGITVIEAPRQSDLMIELIKKLRPQVVVTGIAPFEAVTSSAFEHLLRVTREIGCRLFVDISDQFELSSLPSSIGVLKYLARTPLPSHAAIICGLLKNQVYTDLEVAFVISEEQTTFDALSRSVELLQANTALISQYYYGCLFHELLSFQLPDRRPPSEREAEDVKSSEMIGFSSSAISVLNQSELSVRETENSSLLHMDVDQIFLPTPTPVKAAIFESFARQNVTESECDVTPNLRQFIKNAYNFTAEHTAEFIYADFPLALFNKLVLCCIEEGGSLCIPAGSNGCYLSAAKFLNANIVSIPTQAELGFKLTEKQLSLVLETANKPWVYISGPTINPTGLLYSNAEMKSLLTVCAKYGARVIIDTSFSGVEFNSKGWDGWNLDACLAGLTGKPSFNVCLLGGLFFKIPTGGLAYGFLVLKQGFSADSFHSFLGLNKPHSTIRYTAKKLLELGDLTGVAQQQGILLATRFNRLKQTLENCGWEVIDACGGVSVIAKPSSYLGKSIKLEKDGSTWEAKLNDTNIREAVLRATGLCINGPSWTGIPGYCRFTLALEDGDFDRALDGILKFKQLVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DAT5</accession>
    <name>A0AAD5DAT5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_019295</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000093">
        <property type="protein sequence ID" value="KAI7757643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DAT5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02028">
        <property type="entry name" value="UMPK_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.320.10">
        <property type="entry name" value="Hypothetical Protein Pfu-838710-001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033469">
        <property type="entry name" value="CYTH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006083">
        <property type="entry name" value="PRK/URK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10285">
        <property type="entry name" value="URIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00485">
        <property type="entry name" value="PRK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00988">
        <property type="entry name" value="URIDINKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55154">
        <property type="entry name" value="CYTH-like phosphatases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="929"/>
            <end position="950"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="3">
        <location>
            <begin position="61"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="3">
        <location>
            <begin position="516"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="402"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="434"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="893"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="19"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7757643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="955" mass="106228" checksum="48D7381F59943F26" modified="2024-05-29" version="1">MDDDVVHRVLQEGGRDYYQQTPSTSSSSPSILQSLPLHASFDRGYYLLVKSIQELRSKKEGIVAVGIGGPSGSGKTSLAEKVASVIGCSVISMENYGTGADEGNDLNSIDFDLLVQNLQDLLRGKDILTPVFDFQQKKRVGTKEIKSTSFGVVIVDGTYALHARLRSLLDIRVAVVGGVHFSLLSKVQYDIGDSCSLDNLIDSIFPLFRKHIEPDLHHAQIRINNSFGASFRDSIYKLKCKTESESEQSAYFFPKEEAQIDNFIEMYLRPPSASEEARVNDWIKVRQSGIRYYLSLGDQRIVDKNFIIRPKAEFEVGRMTLGGLLALGYNVVVSYKRASKSIIYGDLSISLETIDTLSETYIVLRGRDRKMVEAEASRLGITSPWITKSYLEMILDSQGVPRLNTPPPMATRPSGINRESPIVAPKPLRIRPDLVNRDDDMTQPWTRSPTKSKMDPVLAPWRFITSDGSVVDGSSIDPSSFRDTLQLAPMPDSFDLDRGLLLAVQATQALLEHKGLPVIVGIGGPSGSGKTSLARKMASIVGCEVISLESYYKSEQLKDFKYDDFRSFDLALLTKNIGDIRRGRKTKVPVFDLESGARSGYKELEVDEDCGVVIIEGVYALHPNIRKSLDLWVAVVGGVHSHLISRVQRDKTKAGCFMSQNEIMTTVFPMFQLHIEPHLVHAHLKIRNDFDPVLSPESSLFVLKSNKQVAYQDILKLLDPAKICSSAQNFIDIYLRLSGIPATGQLTESDCMRVRICEGRFALLIREPIKEGDFIIQPKVDFDISISTVSGLLNLGYQAVAYIEASAYIYQDGKLLIEVDHLNDVPGPYIQIKGANKEVVIAAGSTLELDDSYTTKSYLEIVLEKLPASDRNSNGIYAQQAARLQELVDNIQSQGSSTSSESSPSREVGSPVESVIEDMQSRIKRLERWQAINTVLWTFLMSAFVGYSLYQRKHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DBV2</accession>
    <name>A0AAD5DBV2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pseudouridine synthase</fullName>
            <ecNumber evidence="7">5.4.99.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_013499</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Responsible for synthesis of pseudouridine from uracil.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>a uridine in RNA = a pseudouridine in RNA</text>
            <dbReference type="Rhea" id="RHEA:48348"/>
            <dbReference type="Rhea" id="RHEA-COMP:12068"/>
            <dbReference type="Rhea" id="RHEA-COMP:12069"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the pseudouridine synthase RluA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.-" evidence="7"/>
    <dbReference type="EMBL" id="JAMZMK010000742">
        <property type="protein sequence ID" value="KAI7755871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DBV2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000455">
        <property type="term" value="P:enzyme-directed rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02557">
        <property type="entry name" value="PseudoU_synth_ScRIB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2350.10:FF:000036">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.10">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006224">
        <property type="entry name" value="PsdUridine_synth_RluA-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006225">
        <property type="entry name" value="PsdUridine_synth_RluC/D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006145">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050188">
        <property type="entry name" value="RluA_PseudoU_synthase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00005">
        <property type="entry name" value="rluA_subfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21600">
        <property type="entry name" value="MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21600:SF40">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE RPUSD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00849">
        <property type="entry name" value="PseudoU_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01129">
        <property type="entry name" value="PSI_RLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Pseudouridine synthase RsuA/RluA-like" evidence="9">
        <location>
            <begin position="121"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="305"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="306"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR606225-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7755871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="379" mass="43085" checksum="A863EC28404EF65A" modified="2024-05-29" version="1">MKRNRENMEIVWQTPANPPQRHDYIFRNRIRYVKPYYFEFISHVKNRWAGKTIVDLFADEFKGRNRDYYDSAVKSGRIQVDGKTVSVSYRVQSSQKISHFVHRHEPPVMALDVTVLHKGEDVLTVCKPASVPVHPCGQYRKNTVVGILQAEHDLAPLFPVHRLDRLVSGLLILARSASQADIFRQQIESGMVQKQYIAKVVGEFPKEEQTVNLRVNFNAREGRSTVEIGDPDEKGGNALNGKAACTKFTRICTNGKYSIVSCHPITGRTHQIRVHLQFTGHPIANDTLYLTDYDDGNSRRAFNTVKSSHSSKINSDDNSNNQPEDSIEDFDIDPMCTNCPNLAPIGYDGSEEGLWLHCVRYSGPDWVYECPYPDWASLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DCU9</accession>
    <name>A0AAD5DCU9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Aquaporin TIP4-1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_018441</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Channel protein in tonoplast. These proteins may allow the diffusion of amino acids and/or peptides from the vacuolar compartment to the cytoplasm.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the MIP/aquaporin (TC 1.A.8) family. TIP (TC 1.A.8.10) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000118">
        <property type="protein sequence ID" value="KAI7757454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DCU9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015250">
        <property type="term" value="F:water channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00333">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1080.10:FF:000002">
        <property type="entry name" value="Probable aquaporin TIP1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="entry name" value="Glycerol uptake facilitator protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034294">
        <property type="entry name" value="Aquaporin_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000425">
        <property type="entry name" value="MIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022357">
        <property type="entry name" value="MIP_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00861">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45665:SF26">
        <property type="entry name" value="AQUAPORIN TIP4-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45665">
        <property type="entry name" value="AQUAPORIN-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00230">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00783">
        <property type="entry name" value="MINTRINSICP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81338">
        <property type="entry name" value="Aquaporin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00221">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="99"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="171"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="212"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00053065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7757454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="248" mass="25943" checksum="64265450C7DBA423" modified="2024-05-29" version="1">MAKIAIGSFAEITKPDCIQALVVEFIVTFFFIFAGVGSAMTTEKLAGNEVVGLLFVALAHALVVGVMISAGFRISGGHLNPAVTIGLCAGGHITVIRSLFYWIDQLLASVAACALLSYLTGGLTTPVHTLAAGMDSLQGVIMEIVLTFSLIFTVYATLVDPKKGFLEGLGPLLTGLVVGANIMAGGPFSGASMNPARSFGPALVAGVWTDHWVYWVGPLVGGGLAGFIYETFFVDKTHIPLARDEEGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DEQ8</accession>
    <name>A0AAD5DEQ8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Alpha-soluble NSF attachment protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_028272</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Required for vesicular transport between the endoplasmic reticulum and the Golgi apparatus.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the SNAP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000034">
        <property type="protein sequence ID" value="KAI7758032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DEQ8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005483">
        <property type="term" value="F:soluble NSF attachment protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035494">
        <property type="term" value="P:SNARE complex disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15832">
        <property type="entry name" value="SNAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000049">
        <property type="entry name" value="Alpha-soluble NSF attachment protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000744">
        <property type="entry name" value="NSF_attach"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13768:SF33">
        <property type="entry name" value="NSF ATTACHMENT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13768">
        <property type="entry name" value="SOLUBLE NSF ATTACHMENT PROTEIN SNAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14938">
        <property type="entry name" value="SNAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00448">
        <property type="entry name" value="NSFATTACHMNT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 7" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="111"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7758032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="290" mass="32923" checksum="86CBF9084CD9E602" modified="2024-05-29" version="1">MSDQIAKGLEFEKKAEKKLNGWAFFGSKYEDAGDLYEKAANCYKLAKSWDQAGSVYVKLAECHLKMDSKHEAANAYADAAHSYKKTNTKECIAHLEQAMNLFMEIGRLSMSARYCKEIAELYEQEQNLEQAIAYYDKAADLFQGEEVTTSANQCKQKIAQFSAQLEQYQKAIEIYEEIARQSLNNNLLKYGVRGHLLNAGICQLCKGDVVAITNALERYQELDPTFSGTREYKLLADLAAAMDEEDVAKFTDAVKEFDSMTKLDEWKTTLLLRVKLLLKAKEEEDDDDLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G2N6</accession>
    <name>A0AAD5G2N6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_008011</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011702">
        <property type="protein sequence ID" value="KAI7726355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G2N6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009873">
        <property type="term" value="P:ethylene-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00018">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.730.10:FF:000001">
        <property type="entry name" value="Ethylene-responsive transcription factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.730.10">
        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001471">
        <property type="entry name" value="AP2/ERF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044808">
        <property type="entry name" value="ERF_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31190">
        <property type="entry name" value="DNA-BINDING DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31190:SF301">
        <property type="entry name" value="DNA-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00847">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00367">
        <property type="entry name" value="ETHRSPELEMNT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00380">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51032">
        <property type="entry name" value="AP2_ERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0611">Plant defense</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="7">
        <location>
            <begin position="64"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7726355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="198" mass="22127" checksum="F53E20E47D4480DA" modified="2024-05-29" version="1">MDSSFFYNKNDSQEMLLQGMLPRSSSDLSQHSVSSIPYGILPESSNCDRPSHQATNIISAEKTNYIGVRARSWGKYAAEIRDSTRNGARVWLGTFDTPQEAALAYDQAAYATRGAMAVLNFPVETVCKSLQEMDYRLEEGDSPVLALKRSYSIRRKAMIREKKGNEEEDSKNHVVVLEDLGVDYLEEILSLSESDGSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-04-02" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G7E3</accession>
    <name>A0AAD5G7E3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">SWIRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_002580</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the flavin monoamine oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010483">
        <property type="protein sequence ID" value="KAI7731304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141052">
        <property type="term" value="F:histone H3 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.660.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050281">
        <property type="entry name" value="Flavin_monoamine_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007526">
        <property type="entry name" value="SWIRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035441">
        <property type="entry name" value="TFIIS/LEDGF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742">
        <property type="entry name" value="FLAVIN MONOAMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742:SF410">
        <property type="entry name" value="LYSINE-SPECIFIC HISTONE DEMETHYLASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04433">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00419">
        <property type="entry name" value="ADXRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47676">
        <property type="entry name" value="Conserved domain common to transcription factors TFIIS, elongin A, CRSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50934">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SWIRM" evidence="8">
        <location>
            <begin position="230"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="36"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="331"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1081"/>
            <end position="1136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1254"/>
            <end position="1275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="37"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="87"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="114"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1254"/>
            <end position="1267"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7731304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="1428" mass="156195" checksum="E2DC3D88A3FF54A8" modified="2024-05-29" version="1" fragment="single">AIQVLDSVNACKIDGHWKEEVVLKSGSSFEYTNCRISKPGSSPTSSPKVAANPRDQNVSPSLLVHNGTLDQGSNPIGGVGLDLLQNGLHSTQPHTQKEVSSSDCDSPNHGIASVASQKENASTSDGRLSPRSLGECNKSADENEAYASETGSMLERETKSNKKSAAKRIVRQAKRHRHDDMAYEGDADWETLIHEQNFLVNHQDGDKGQSTKTKGKLDFLQTISLETESGGVAAVSVGLKARSAGPVEKIKFKELLKRKGGLQEYIECRNHILHLWNKDVTRILPLSECGISDTNVEDEHPQASLIRDIYSFLDQYSYINFGVASKKEISDSGAKPNFRLSGDENDGRKSGAPVTDLDDGVSFILGKTKNYDNLTDEIDTATEALDEVVKKDKDVEVLDSLCESVVNNCESVKSPNRLYTQVEDSVDQKEPTVCMQCDDSETRKKIIVIGAGPAGLTAARHLNRQGFHVTVLEARDRIGGRVYTDHSSLSVPVDLGASIITGVEADVTSQRRPDPSSLICAQLGLELTVLDSDCPLYDTVTGQKVPSDLDEALEAEYNSLLDDMQLVVAKNGDRAMQMSLEEGLEYGLKIRRVGTAKTESGNEILSPLERRVMDWHLAHLEYGCAASLQQVSLPYWNQDDIYGGFGGAHCMIKGGYSAVVDSLKDGLHIHLNHMVTDICYQQEDESQNKVKVFTKNGKIFTGDAVLVTVPLGCLKAETIKFSPSLPEWKNSSIKRLGFGVLNKVVLEFPEVFWDESVDYFGATAEETDQRGWCFMFWNVKKTVNAPVLIALVVGKAAIGDQDLTPSDHVNHALVVLRKLFGEGAVHDPVASVVTDWGRDPFSYGAYSYVAVGASGEDYDTLGRPVNNCLFFAGEATCKEHPDTVGGAMMSGLREAVRIVDILTTGNDFTAEVEAIAAAKRHSDTERSEVRDIIRRLDAIELTGAHKSSLLQNMFSNAKSRAGRLHLAKELLNLPSDVLKSFAGTKEGLGILNSWILDSMGKNGTQLLRQCVRLLVLVSTDLLAVRLSGIGKTVKEKVCVHTSRDIRAIASQLVSVWVEIFRKEKASNGGLKLLRQSSADASKIKSNSASGKPPLRGHHASSDNRAVKGVSVNTHNRKSVTKPEHKPSSSLGSAGKQYCREEEYNDVPLSEEEQAAFDAAEAARAAAIAAAEAYESSGVKCSTPLLLPKIPSFEKFARREQYAQMDESEFRKKWAGGVTGRQDCLSEIDSRNCRVRDWPVDFSAAGVNIESSKISVDNRSQRSNSNENAGPLSYKEYSGESTGVDKSIFTKAWVDSAGSEGLKDHSAIERWQSQAAAADSEFYHRSMHAMDEEYSNMNLKPSIHRHDGLANESSASQVTVKRELVGNQPRGVDKIKQSVVDYVASLLMPLYKARKLLKSTNFFVQIRAFVDMLIDRHMATKTDAKSGCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-04-02" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G7F7</accession>
    <name>A0AAD5G7F7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA-3-methyladenine glycosylase I</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_002553</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010483">
        <property type="protein sequence ID" value="KAI7731277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008725">
        <property type="term" value="F:DNA-3-methyladenine glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.30">
        <property type="entry name" value="Hypothetical protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005019">
        <property type="entry name" value="Adenine_glyco"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31116:SF25">
        <property type="entry name" value="DNA GLYCOSYLASE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31116">
        <property type="entry name" value="OS04G0501200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03352">
        <property type="entry name" value="Adenine_glyco"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="DNA-glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="31"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="78"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="299"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="303"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR605019-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7731277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="339" mass="38150" checksum="8983624A1DDD00F8" modified="2024-05-29" version="1">MSASPDLLSIPPSVKTDSASRKILGPAGNTEKIHKDDNLKNKKKKKIVVETKAKSSVNLAKGSRSPRTVVRKNDESVDSSCSLKSCSSNGSGSVKSKTAGSERVKKDDGNRLDEAQVMVLNIPFKRCDWITRFSDPLHVSFHDEEWGVPVYEDCKLFELLVLSLASAELSWTELLYKRDKFRKLLENFDPALIAKFSEDRLLSAKQNGSLLLSEXXXXIMANAIGFIKIQQEFGSFSSYCWRFLNKKPINNSFRYARQVPSKTPKSEHISKDLMKRGFRCVGPTVIYSFMQISGMVNDHLISCFRHMECSSIAKKVLKPVSKETKQETDNIDDSKLSHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G7U2</accession>
    <name>A0AAD5G7U2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Tubulin alpha chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">M8C21_025242</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">AA19_3_7</strain>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11 13">Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="13">Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the tubulin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010399">
        <property type="protein sequence ID" value="KAI7731709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G7U2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02186">
        <property type="entry name" value="alpha_tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.600:FF:000005">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.20:FF:000001">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000004">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.600">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002452">
        <property type="entry name" value="Alpha_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023123">
        <property type="entry name" value="Tubulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01162">
        <property type="entry name" value="ALPHATUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9 13" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 13" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="15">
        <location>
            <begin position="14"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="16">
        <location>
            <begin position="213"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="383"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAI7731709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="415" mass="45993" checksum="150920A00C876555" modified="2024-05-29" version="1">MTSGSSEASGDDSFNTFFSETSSGKNVPRAVFVDLEPTVIDEVRSGTYRQLFHPEQLISGKEDAANNFARGHYTVGKEIVDVCLDRVRKLADNCTGLQGFMVFNAVGGGTGSGLGSLLMERLSIEYGKKPKLGFSIFPSPQVSTAVVEPYNSVLSTHSLLEHTDVVALLDNEAIYDICRRALDIERPTYTNLNRLISQIISSLTTSLRFDGAINIDINEFQTNLVPYPRIHFMLSSYAPVISSARAYHEQNSVSEITTAVFEPSNMMAKCDPRHGKYMACCLMYRGDVVPKDVNTAVGSIKTKRTVQFVDWCPTGFKCGINYQPPTVVPGGDLAKTARAVCMISNNTAMAEVFNRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGLDADEEEEQGEEEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G7Y2</accession>
    <name>A0AAD5G7Y2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Inositol-tetrakisphosphate 1-kinase</fullName>
            <ecNumber evidence="9">2.7.1.134</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_011337</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Kinase that can phosphorylate various inositol polyphosphate such as Ins(3,4,5,6)P4 or Ins(1,3,4)P3.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>1D-myo-inositol 3,4,5,6-tetrakisphosphate + ATP = 1D-myo-inositol 1,3,4,5,6-pentakisphosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12452"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57539"/>
            <dbReference type="ChEBI" id="CHEBI:57733"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.134"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9 11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="9 11">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 9">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the ITPK1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.134" evidence="9"/>
    <dbReference type="EMBL" id="JAMZMK010010377">
        <property type="protein sequence ID" value="KAI7731814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G7Y2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052726">
        <property type="term" value="F:inositol-1,3,4-trisphosphate 5-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052725">
        <property type="term" value="F:inositol-1,3,4-trisphosphate 6-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047325">
        <property type="term" value="F:inositol-3,4,5,6-tetrakisphosphate 1-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032957">
        <property type="term" value="P:inositol trisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008656">
        <property type="entry name" value="Inositol_tetrakis-P_1-kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040464">
        <property type="entry name" value="InsP(3)kin_ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041429">
        <property type="entry name" value="ITPK1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14217">
        <property type="entry name" value="INOSITOL-TETRAKISPHOSPHATE 1-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14217:SF39">
        <property type="entry name" value="INOSITOL-TETRAKISPHOSPHATE 1-KINASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05770">
        <property type="entry name" value="Ins134_P3_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17927">
        <property type="entry name" value="Ins134_P3_kin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038186">
        <property type="entry name" value="ITPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 9 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 9 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Inositol-tetrakisphosphate 1-kinase N-terminal" evidence="13">
        <location>
            <begin position="36"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="Inositol 1,3,4-trisphosphate 5/6-kinase ATP-grasp" evidence="12">
        <location>
            <begin position="135"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>1D-myo-inositol 1,3,4-trisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58414"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>1D-myo-inositol 1,3,4-trisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58414"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>1D-myo-inositol 1,3,4-trisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58414"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="199"/>
            <end position="210"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>1D-myo-inositol 1,3,4-trisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58414"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="290"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="305"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="305"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="307"/>
        </location>
        <ligand>
            <name>1D-myo-inositol 1,3,4-trisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58414"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="307"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="311"/>
        </location>
        <ligand>
            <name>1D-myo-inositol 1,3,4-trisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58414"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR038186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR038186-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR038186-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF05770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF17927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7731814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="342" mass="38694" checksum="E1F8F22F2E04560B" modified="2024-05-29" version="1">MRLMQSEEEREMESSSDCGSIVELGFQDRSKKVVVVVGYALTSKKIKSFLQPKFEGLARNKGIFFVPIDQTRPLSDQGPFDIVLHKLSGKEWQQILEDYRLTHPEVTVLDPPDAIQHVHNRQSMLQDVADLDFSDAYGTVGVPKQLVIEKDPSSISNAVKKAGLTLPLVAKPLVAKSHELSLAFDEYSLEKLEPPLVLQEFVNHGGVLFKVYIVGDAIKVVRRFSLPDVSKRELSRSVGVFRFPRVSSAAQSADDADLDPCIGELPPRALLERLARELRRRLGLHLFNLDMIREHGTKNRFYVIDINYFPGYGKMPEYEHIFTDFLLNLAKSKYKKRSASIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G833</accession>
    <name>A0AAD5G833_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_001640</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010192">
        <property type="protein sequence ID" value="KAI7732640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G833"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7732640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="77" mass="8659" checksum="D42223BA37421C48" modified="2024-05-29" version="1">MNEVCVRQSLHRKSINLQPQTSFLEDHGGPWKLVTTGIFYIVNQVSKVVNEGGGKRRCCGGAKKRVTRGGYGCHKRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G8W2</accession>
    <name>A0AAD5G8W2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">adenylate kinase</fullName>
            <ecNumber evidence="2">2.7.4.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">ATP:AMP phosphotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_033995</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the adenylate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.3" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010009973">
        <property type="protein sequence ID" value="KAI7733465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G8W2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004017">
        <property type="term" value="F:AMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000850">
        <property type="entry name" value="Adenylat/UMP-CMP_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23359">
        <property type="entry name" value="NUCLEOTIDE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00406">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00094">
        <property type="entry name" value="ADENYLTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7733465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="74" mass="7612" checksum="51292F743EB3FCBE" modified="2024-05-29" version="1" fragment="single">MGTVVDAADMEGTKSALSEKHPKIVFVLGGPGSGKGTQCANIVENFGYTHLSAGDLLRAEAQSGSENGAMIEKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G9B1</accession>
    <name>A0AAD5G9B1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_021325</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010027">
        <property type="protein sequence ID" value="KAI7733332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G9B1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7733332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="81" mass="8799" checksum="F925F38461E51C6F" modified="2024-05-29" version="1">MIDVMCATPLHNYGAFLEKDGAGFEGQVTLPGFKNGDVDLIHSSCTYQEVQMGKKSWFSAMKKALSPSTSKTTKTKKVIPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GA79</accession>
    <name>A0AAD5GA79_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein YIP</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_004939</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009569">
        <property type="protein sequence ID" value="KAI7735070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039765">
        <property type="entry name" value="Yip5/YIPF1/YIPF2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12822:SF5">
        <property type="entry name" value="PROTEIN YIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12822">
        <property type="entry name" value="PROTEIN YIPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="120"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="165"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="193"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="217"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7735070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="244" mass="26479" checksum="8C8167A8BB0F43B5" modified="2024-05-29" version="1">MTEPSYTSLPTSHLPGSVPAVITEERASAIQKDPEANMQIFPPNSGGGGGGGGGGRGYQTLPGPSDGNGPTPANNWNGTFSVSSYTQYFNVDTDDVVNRLTSSLYPTGDFFRKIEANPDLYGLIWISTTLVFVIASFGNCATYLTSKKTDSSISWTFDVNYFQSWPYPLLVHXXXXFIISSLLLVIPVDFVRWTITLITGVASAAFVGLNLKSHVELNDLTIVLVAACVLQFALAIFIKSWFFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GAE5</accession>
    <name>A0AAD5GAE5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Plant intracellular Ras-group-related LRR protein 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_024979</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010099">
        <property type="protein sequence ID" value="KAI7732988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GAE5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000116">
        <property type="entry name" value="Leucine-rich repeat-containing protein 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF1">
        <property type="entry name" value="RAS SUPPRESSOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7732988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="609" mass="67026" checksum="C14436652E8E4DC1" modified="2024-05-29" version="1">MDRMLKAARASGSLNLSNRSLKEVPDEVYKSLDAVGEGENWWEAVELQKLILAHNDIQLLKEDIRNLPLLSVLNVSHNKLSNLPTAIGELKSLKALDVSNNLLCEIPEEVGSATALIKFDCSSNQIKDLPCSLGNCLDLSDLKASNNRLTSLPEDLGNCSKMSKLDVEGNKLTTLSEKLVASWTSLTELNAGNFLRCNSITGDAAKNTLGDIPESIGSLSRLIRLDLHQNRIASIPSSIKGCSLLAEFYMGNNFLTSLPTEIGELMNLGSFDLHSNKLKEYPVEACKLKLSVLDLSNNTLSGLPPEIGKHPFMVIVSYIMRRLMTTLRKLVLTGNPIRTLRSSLVTGPTPALLRFLRSRLPAEEESGPSESKENIIARASRLSLGSKELCLGGHGLSDVPPQVWETSDITKVDLSRNSIEDLPVQLSSCASFETLILSRNKIKEWPSAILESLQNLVCLKLDNNPLRQIPSDGFQAVSKLQILDLSGNAGCLPENPAFSVLMQLQELYLRRMQISEIRADIISLPKLRILDMSQNSLQLVPEGFKNATSLQELRLSDNNISTLPPELGLLEPSLQVLQLDGNPLRSIRRTILDRGTKAILAYLKDKLPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GAH8</accession>
    <name>A0AAD5GAH8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">OTU domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_033031</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C85 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009488">
        <property type="protein sequence ID" value="KAI7735430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GAH8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061578">
        <property type="term" value="F:K63-linked deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22796">
        <property type="entry name" value="OTU_plant_OTU6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.80:FF:000008">
        <property type="entry name" value="OTU domain-containing protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003323">
        <property type="entry name" value="OTU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050704">
        <property type="entry name" value="Peptidase_C85-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12419">
        <property type="entry name" value="OTU DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12419:SF116">
        <property type="entry name" value="OTU DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02338">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50802">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OTU" evidence="3">
        <location>
            <begin position="195"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="395"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="9"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="86"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="108"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="120"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="403"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7735430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="476" mass="52801" checksum="2F70147565E87591" modified="2024-05-29" version="1">MTRVFVQRGSGSSSSGSSNNNNNNTNRQSGQVTGEDVEQQQQQIVSEDVVVVEMETVDKKSDDLDVTGNDKVEHEAVGTGTLRVIEEEEKDGLETDDGSAADVGGSQYPPPPPAPPPKPSSMSLNSRRFGNSSRRATAWPVVNARASTVETRSSSHCENDGYNSADEQNTCYGSSYGDAERERQFELDIRRTKGYEVKRMLEDGNCLFRAVADQVYGDSEAYDLARQMCIDYMERERDHFSQFITEGFTSYCKRKRRDKVYGNNVEIQALSEMYNRPIHIYSYSTEPINIFHGSYNTDTPPIRLSYHHGNHYNSLVDPRRLTVGAGLGFSSLQGANVDKDQVKAAIKAQQDQQIDNALLAEARFCSDVELTEKEIERMVMEASRAEYMANDIKQQLGRRESSTSDAEPSSSGGRPSGSESKGSEGSVLCSSMQMVLSMGFSYVQAMEAYSIFGDDVDSMVCYLLETSSRRKGKATE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GAM3</accession>
    <name>A0AAD5GAM3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_025475</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009633">
        <property type="protein sequence ID" value="KAI7734847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GAM3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7734847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="47" mass="5513" checksum="543E572A11B0B59E" modified="2024-05-29" version="1">MVKDGFAGDDEQCLLALLDIRDTLESWLVWDKRMHTLVTRHNPNVIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GCM5</accession>
    <name>A0AAD5GCM5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Transcription factor MYB1R1-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_028048</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009236">
        <property type="protein sequence ID" value="KAI7736569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GCM5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009723">
        <property type="term" value="P:response to ethylene"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009739">
        <property type="term" value="P:response to gibberellin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000009">
        <property type="entry name" value="transcription factor MYB1R1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006447">
        <property type="entry name" value="Myb_dom_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052245">
        <property type="entry name" value="Plant_Stress_Dev_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01557">
        <property type="entry name" value="myb_SHAQKYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44191:SF84">
        <property type="entry name" value="F25A4.19 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44191">
        <property type="entry name" value="TRANSCRIPTION FACTOR KUA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="8">
        <location>
            <begin position="114"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="6">
        <location>
            <begin position="114"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="7">
        <location>
            <begin position="117"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7736569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="288" mass="32362" checksum="929C0E000908FE90" modified="2024-05-29" version="1" fragment="single">SLSINNTCTPSPFILDNNNIFIINIITPYYLYPYLSPFLPHFNVIRRNIMELESQSDAGEIMLFGVRVKVDPMRKCVSMSNLSQYESSNNILVGNTTGYASADDTVRHQSNGSRDRKRGVPWTEDEHKLFLQGLQKVGKGDWRGISRNFVKTRTPTQVASHAQKYFLRRTNLNRRRRRSSLFDITTDSVAATMQTEEQINENNKVQAVVQPSMPPPPVQLQKPAEMMTLNNVGQVDGGSMQIVRPFTAVPAANPSARMDLDLSLTYNPNRSGSYQVITGFNNGDSIIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GCQ5</accession>
    <name>A0AAD5GCQ5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Polygalacturonase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_017532</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the glycosyl hydrolase 28 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009496">
        <property type="protein sequence ID" value="KAI7735358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GCQ5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004650">
        <property type="term" value="F:polygalacturonase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000743">
        <property type="entry name" value="Glyco_hydro_28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31375">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00295">
        <property type="entry name" value="Glyco_hydro_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00710">
        <property type="entry name" value="PbH1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00502">
        <property type="entry name" value="POLYGALACTURONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0134">Cell wall</keyword>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6 9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042221955" description="Polygalacturonase" evidence="10">
        <location>
            <begin position="25"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7735358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="444" mass="47554" checksum="BCD1087E5B20E1DC" modified="2024-05-29" version="1">MKICMFGLLTCVLVLFLQHQGVETRHGHGSRLLKETFHPRYEETILSSLPYGNTSTIFNVLAFGAKGDGTTDDTKAFEAAWLAACKLEASTMIVPNGSVFLIKPISFSGRNCESNIIFQLDGKIIAPKRSGSWKSGLLQWLEFRKLNGITICGTGVIDGQGAGWWSKSSGTKPTAVRFYGSSNVTVTGITIQNSQQTHLKFDNCQSVQVFDVTISSPGDSPNTDGIHLQNSQDVTINSTRLACGDDCVSIQTGCSGINIHNVDCGPGHGISIGSLGKHNTKACVSNITVRDTMIHDTENGVRIKTWQGGYGSVQGVTFSNIQVSNVEIPIIIEEYYCDGGKCQNKGSSVDVSDISYQNIKGTYTKTPIRFACSESTPCTDVNLDTIQLQPVAAVVRGVQRKAAVVMGLQPDAAEPLLEQPGKEGWAGCVEQRLENAAGTGCSAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GDF8</accession>
    <name>A0AAD5GDF8_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_023681</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009288">
        <property type="protein sequence ID" value="KAI7736376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GDF8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7736376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="92" mass="10600" checksum="7D47C611373148DF" modified="2024-05-29" version="1">MMQIVSTNHASMLIETSGHWSSGCFCALNEPEEYEDNNLLFESKFVNFDLDGVYRNATWQGGYGQRYGSLAKEEKYDIFPDIKEYADVNLKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GEH9</accession>
    <name>A0AAD5GEH9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_009027</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009096">
        <property type="protein sequence ID" value="KAI7737188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GEH9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019141">
        <property type="entry name" value="DUF2045"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21477:SF13">
        <property type="entry name" value="KIAA0930"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21477">
        <property type="entry name" value="ZGC:172139"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09741">
        <property type="entry name" value="DUF2045"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7737188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="342" mass="37737" checksum="EBB8CB23FE1DFE92" modified="2024-05-29" version="1">MKADMFGYQLSVKSVRLGYWSLLNVRCELLSMVKKHSSLIGKTVLDEQDASDVELDQKFWHDILDLYFIGGRDAKEQQDDDLLFFVKNLSLHGYGFNDNLEGDSPYFVRRWAATKLDDLVGESSVIVDWRRSLYLNLIAHTSFSVTVAICSYQSLQNYQNNQGTPLSPIYKEVETKPAYPDICFAVDDFDSTFDAVLTLFSGFVSYQMVREAYDAGKSGFGSLLPFGHSARKTDRIYMKGPGGRGEVEVAVSGVVDQSLDEPGPHSPLHISQGGLKIGAMVRKAASAAQVAAKNAYAAASSTRNSDGGTIMVPLKCCLMSISLPWENIAYDLLFKGDSPVNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GG09</accession>
    <name>A0AAD5GG09_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Lysine-specific demethylase JMJ25-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_022471</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the JARID1 histone demethylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008281">
        <property type="protein sequence ID" value="KAI7741042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GG09"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000118">
        <property type="term" value="C:histone deacetylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032454">
        <property type="term" value="F:histone H3K9 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02208">
        <property type="entry name" value="cupin_RmlC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045109">
        <property type="entry name" value="LSDs-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018866">
        <property type="entry name" value="Znf-4CXXC_R1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12549">
        <property type="entry name" value="JMJC DOMAIN-CONTAINING HISTONE DEMETHYLATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12549:SF37">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE JMJ26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02373">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10497">
        <property type="entry name" value="zf-4CXXC_R1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="9">
        <location>
            <begin position="335"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="JmjC" evidence="10">
        <location>
            <begin position="751"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="38"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="129"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="211"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="278"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="69"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="144"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="281"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="309"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7741042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1028" mass="116528" checksum="55E910E11B9B873A" modified="2024-05-29" version="1">MEVVFPKENQMSRYATIATNEINVRQKVLTIVPELERKRKVQKLMNSQKGDDQGSSKKRTRASSHKEMSSSSSGPSKSCFSGESEEENANVQFRKGTLTTKTKKITMTVNTRGIKGYYKIAKCSLSKKTTSNEKLNSKGDSNEKASSNKKQCVTTTNCEDNLEDEELIDSKKSKQNDVISSPHDVLLSRSRRVVSTENKLNKDGMLYVDWEYDEENEYGTGDEEEEEEDDDDDDSDYDADENVLLTSMELHELNNVNSKGSIKGRRVEKDVDFIEEKKQMSFMKSNPSRSSSSNDTIMKRRNKDTGDCSSASSGGTPSTSINVNDQKNGNEHLKCHQCKRNDRKIVVPCIKCDKIVYCIQCIRQWYPQLEEEEIAELCPYCRGNCNCNLCLHANIKMPSIDLTDTEKLQHLHYVISSLLPFVRQIREEQDKEIAVEARIRGLPESSVTIWPTTCSTEERQLLFNINHRSSSCPTCSYELCLSCCHEIRNTGLVGLRKVAFGYSNRGSDYIHGGDPPQNSLHVNSPNGHCDSEVKWVAKDDGSLFCAPKEMGGCGDCLLELKHILQEDWLSTLEAKAELLLNKFKIEQPNFMTSSFTTDGETYLKAANREESNDNHLYYPTSEEALSGEELIRFRHHLAKGEPVIVRKVLDQTPGLSWEPTVMWRALCEHVDPNVSSKMSQVKAIDCLAGCEVEISTRKFFKGYEEGRQYKNSWPEMLKLKDWPPSDKFEDLLPRHCDEFISALPFQVYTDPRAGFFNLAVKLPPGVLKPDLGPKTYIAYGMAEELGRGDSVTKLHCDMSDAVNILTHTAEVSVSDDQKIAIQELKSRHRAQDEREKNGTLFECVAGSSVKKEELSTVKEEEYNNASSINKEVVTCDQPSEVSHDFVPQADASEETAGALWDIFRREDATKLQEYLLKHSKEFRHIYCCPVEQVYHPIHDQSFYLTLEHKQRLKEEYGIEPWTFEQSLGEAVFIPAGCPHQVRNLKRSFKINLLILYDGDNGVGCYCRGCSSNKADKLNAILEEAHMMC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GG58</accession>
    <name>A0AAD5GG58_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_011892</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008220">
        <property type="protein sequence ID" value="KAI7741310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GG58"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBC core" evidence="2">
        <location>
            <begin position="59"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7741310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="196" mass="22701" checksum="2CF9F3FC64CC3ABA" modified="2024-05-29" version="1" fragment="single">PTTKRERDNRDDLGGTPTAKAELRRHHSSRPSVPYPSFILQSELMDLMMRDDTSGIRFSDYPFKPPKVKFETGCFHPNVDVFGNICLEFFQDTQPVMVANYTTSWCIPMWSLTPSKKTWPRPAHVSEIDPFVEVEHYNNNHSTPAVFYNQSLRALYVHRISVLRPVYQSYSDGHLLLSPKTSWTVKILIKSRQPLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GH15</accession>
    <name>A0AAD5GH15_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Hexosyltransferase</fullName>
            <ecNumber evidence="5">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_006890</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1 5">Glycan metabolism; pectin biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Golgi apparatus membrane</location>
            <topology evidence="5">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the glycosyltransferase 8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="5"/>
    <dbReference type="EMBL" id="JAMZMK010008312">
        <property type="protein sequence ID" value="KAI7740979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GH15"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047262">
        <property type="term" value="F:polygalacturonate 4-alpha-galacturonosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06429">
        <property type="entry name" value="GT8_like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029993">
        <property type="entry name" value="GAUT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002495">
        <property type="entry name" value="Glyco_trans_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32116:SF74">
        <property type="entry name" value="GALACTURONOSYLTRANSFERASE 10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32116">
        <property type="entry name" value="GALACTURONOSYLTRANSFERASE 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25557">
        <property type="entry name" value="GAUT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01501">
        <property type="entry name" value="Glyco_transf_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="3 5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="522"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7740979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="522" mass="60248" checksum="04265A9D034F1F04" modified="2024-05-29" version="1" fragment="single">FIYIIQEDNEKTKTRVQAPRQTTVFQFEKLLNPDPDPDPSCLRNDKAMEGLNVTAEMLNPDSITRQLNDQISLAKAFVVIAKESNNLQFAWELSAQIRNSQILLSNAALRRSPLTLRESETAVRDMALLLFQAQQLHYDSSTMIMRLKARIQDLEEQMSSVTDKSNKYGQIAAEEVPKSLYCLGVRLTTEWFKKPDLQKKFSRNGQIGMKLKDNNLYHFCVFSDNILATSVVINSTASNSLHPDRLVFHLVTDEVNYAAMKAWFTMNNFHGVTVDVQKFEDFTWLNASYVPVLKQLQDSETQSYYFSGSNDGGKTPIKFRNPKYLSMLNHLRFYIPEVFPSLKKLVFLDDDVVVQKDLSGLFAIDLKGNVNGAVETCMETFHRYHKYLNYSHPLIRSHFDPDACGWAFGMNVFDLVEWRKRNVTGIYHYWQEKNVDRTLWKLGTLPPGLLTFYGLTEPLDPSWHVLGLGYTNVDSQVIEKGAVLHFNGNSKPWLKIAMEKYKPLWEKYVDFSHPMLERCNFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GI67</accession>
    <name>A0AAD5GI67_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Dof zinc finger protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_000651</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Transcription factor that binds specifically to a 5'-AA[AG]G-3' consensus core sequence.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008266">
        <property type="protein sequence ID" value="KAI7741131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GI67"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045174">
        <property type="entry name" value="Dof"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003851">
        <property type="entry name" value="Znf_Dof"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31992">
        <property type="entry name" value="DOF ZINC FINGER PROTEIN DOF1.4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31992:SF285">
        <property type="entry name" value="DOF ZINC FINGER PROTEIN DOF4.6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02701">
        <property type="entry name" value="Zn_ribbon_Dof"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01361">
        <property type="entry name" value="ZF_DOF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50884">
        <property type="entry name" value="ZF_DOF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 8 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3 9" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 8 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Dof-type" evidence="11">
        <location>
            <begin position="43"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="18"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="84"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="262"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="19"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="98"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="264"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU369094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7741131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="295" mass="32199" checksum="3A09838206FBBBB5" modified="2024-05-29" version="1">MDTVHWPQMKPMEEIAVQNPKNTCPKPTSSLERKPRPPKESAVNCPRCNSINTKFCYYNNYSLSQPRYFCKTCRRYWTEGGSLRNVPVGGGSRKNKRSSSSNSISSHSSAMSKKLPDLVVPPAPPTLLSQNHSRMMHHEHGHDLNLGFPPSDDFKTISELIQVPGFDGSTKNTNSGGSVQLSALELLTGITTRGVMNSFMPIPIPDPNSAYSSSSGELNMIPSLNFSLDGMGGSSGIHESSSGRLLFPFEELKTSTIATTTTATHDDGHHNVEQHRDQNGDTNGFWSGMLGGGSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GID8</accession>
    <name>A0AAD5GID8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_000831</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008207">
        <property type="protein sequence ID" value="KAI7741371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GID8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043539">
        <property type="term" value="F:protein serine/threonine kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06610">
        <property type="entry name" value="STKc_OSR1_SPAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000208">
        <property type="entry name" value="serine/threonine-protein kinase BLUS1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047173">
        <property type="entry name" value="STRAD_A/B-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48014:SF10">
        <property type="entry name" value="PROTEIN KINASE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48014">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE FRAY2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="2"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="384"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="421"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="564"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="384"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="596"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7741371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="596" mass="67048" checksum="D8854DC3944F45DF" modified="2024-05-29" version="1" fragment="single">DYKLYEEVGEGVSASVYRALCVPLNEIVAIKVLDLEKCNNDLSYCSFTTGHKLWVVMPYMSGGSCLHIMKSSFPDGFEEPVIATLLREVLKALVYLHAHGHIHRDVKAGNILVDFNGSIKLADFGVSACMFDAGDRQRSRNTFVGTPCWMAPEVMQQLHGYDFKADIWSFGITALELAHGHAPFSKYPPMKVLLMTLQNAPPGLDYERDKKFSKSFKDMVAACLVKDPKKRPSSEKLLKHPFFKHAKTADYLQRSILDGLSPLGDRFRTLKAKEADLLLQNKELYEDKEQLSQQEYIRGISAWNFNLEDIKNQAALINEYDEISNLEEPKASDQRNGINDNYLDAKSQDTADEIVNLESSFASFPTQSLQALQGCFDICEDDVSTASQTEQPPVQQSLKASDQETDKDEIKQLGRIKSLQKTSIPGPRKYMSGSLLPDNVISPKNSPKKLMSNGERDLHQLKFQERSNSGPLQHMQRNNLTNSLSVENVSEEAVVQRKGRFRVTETDLSPKAPVLNPTPTIGVPAASLLPSLQSILQHNTIQRIPPTPRERELQSQVIQLQQSVVSLVEQLQKQKMRNVQLEKKLSDLMKKNSIEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GIY5</accession>
    <name>A0AAD5GIY5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Methyltransferase</fullName>
            <ecNumber evidence="10">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_023786</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Membrane</location>
            <topology evidence="10">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the methyltransferase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="10"/>
    <dbReference type="EMBL" id="JAMZMK010007514">
        <property type="protein sequence ID" value="KAI7744520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GIY5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000123">
        <property type="entry name" value="Putative methyltransferase PMT15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004159">
        <property type="entry name" value="Put_SAM_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10108:SF1058">
        <property type="entry name" value="METHYLTRANSFERASE PMT18-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10108">
        <property type="entry name" value="SAM-DEPENDENT METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03141">
        <property type="entry name" value="Methyltransf_29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 10" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU366043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7744520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="512" mass="59419" checksum="2B35AB6FB6B55E72" modified="2024-05-29" version="1">MLKYRERHCPSKEELLHCLIPAPPKYKLPFKWPESRDYAWYDNIPHKELSIEKDRQNWIKVEGDRFRFPGGGTMFRYGADSYIDDINELIPLSNGTIRTAIDTGCGVASWGAYLLKRDIITMSFAPRDTHEAQVWFALERGVPAMIGIMGSQRLPYPSRAFDMAHCARCLIPWSKYGGLYLIEVDRVLRPGGYWILSGPPIQYKKLWRGWERSREDLKHEQEEIEDVAKRLCWKKVIEKGALAVWQKPINHIDCIKSKKKHYIKPLICKSGNADEAWYKELEGCITPLPEVSGQDEVSGGALAKWPERAMAVPPRISNNFVRGITEEKFHEDNKKWKERVTHYKRVVTSLHQGRYRNILDMNAYIGGFSAALMEYPVWVMNVVPVNFQPDTLGVIYERGFVGVYHDWCEAFSTYPRTYDFIHADGVFTMYQDRCDIIDILLEMDRILRPEGTVVFRDEPNVIGKIKSIAERMRWNTKTVDHESRPHKGEKILVAVKTYWTGRVANGRVMKLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GJC6</accession>
    <name>A0AAD5GJC6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_030220</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007758">
        <property type="protein sequence ID" value="KAI7743274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GJC6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000032">
        <property type="entry name" value="Serine/threonine-protein kinase PBS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050823">
        <property type="entry name" value="Plant_Ser_Thr_Prot_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45621">
        <property type="entry name" value="OS01G0588500 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="1"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7743274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="241" mass="26111" checksum="A7E11D7C91D2D5E5" modified="2024-05-29" version="1">MRIALGAAKGLAFLHNAERPVIYRDFKTSNILLDSDYTAKLSDFGLAKAGPQGDETHVSTRVMGTYGYAAPEYVMTGHLTARSDVYSFGVVLLELLTGKKSVDKTRPSKEQSLVDWARPKLNDKRKLLQIIDPRLESEYSVRGAQKACSLAYYCLSQNPKARPLMSDVVETLEPLQSGSSSCANEVSLASSLIGVGRPLVIGNHRAIQMHHKFVQTVGAGAGCRSTNPSCSPGPPTACRVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GLN6</accession>
    <name>A0AAD5GLN6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">M8C21_028163</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">AA19_3_7</strain>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010007581">
        <property type="protein sequence ID" value="KAI7744133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GLN6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056760">
        <property type="entry name" value="RING_XB3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24161">
        <property type="entry name" value="ANK_REP_REGION DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24161:SF85">
        <property type="entry name" value="PALMITOYLTRANSFERASE HIP14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13637">
        <property type="entry name" value="Ank_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24921">
        <property type="entry name" value="RING_XB3-XBAT31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10 11" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="repeat" description="ANK" evidence="11">
        <location>
            <begin position="44"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="11">
        <location>
            <begin position="77"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="11">
        <location>
            <begin position="111"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="11">
        <location>
            <begin position="174"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="11">
        <location>
            <begin position="217"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="14">
        <location>
            <begin position="313"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="441"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="463"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="15">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAI7744133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="482" mass="52268" checksum="000FBCFDB9D81B80" modified="2024-05-29" version="1" fragment="single">MGNSFGCSATGERLVSAARDGDVQEAKALLEYNPRLARYSTFGVRNSPLHYSAAQGHHEIVSLLIQSGVDINLRNYRGQTALMQACQYGHWEVVLTLVLYKANIHKADYLNGGTALHMAAFSGHTRCIRILLADYIPGVPNFYNFIIKRSRVKESGLEFDEGSLIETINRPADGGLTALHMAALNGHVDSLQLLIDLGASVNKVTVEDGTTIDLIGAGSTPLHYAACGGNAQCCQILIARGASITAENAKGWTPLAVARLWHREWLEEVLIAQLQDEEPTSSPYWCLPLMSVVKIARECGWSNDAISACADPCAVCLENKCALAADGCHHEFCTRCALYLCSTSTSSNASQGPPGSIPCPLCRQGIISFRKLPGTRPLLGMPRTSLPLSFFTCTSLTEDTDNTTLDTTPLCKPEFPYSLSSLSCQKFPSMKLSSGLCMGTSDTGPSLSRPSVDRGRFSRSSFRRSDSHSDSRRSWLCSLNQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GMC7</accession>
    <name>A0AAD5GMC7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_007093</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006587">
        <property type="protein sequence ID" value="KAI7748165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GMC7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7748165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="129" mass="14520" checksum="4C40224BC6D08A91" modified="2024-05-29" version="1">MIPRVGELCVLQTMLRIKIGSYNSVDTRSQVMVHPLVNLCRDPNIHGILGVGWGVEDVGDDDSSHVTFRWIPARYVDVKGINMCPFTVGGVTAASTAKYWWSTMINNLYTCSQLRAYYVTQVQKQLNTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GMG6</accession>
    <name>A0AAD5GMG6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_003886</name>
        <name evidence="1" type="ORF">M8C21_033879</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006568">
        <property type="protein sequence ID" value="KAI7748285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAMZMK010000864">
        <property type="protein sequence ID" value="KAI7755725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GMG6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7748285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7755725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="94" mass="10009" checksum="7AA1A2A344C5F618" modified="2024-05-29" version="1" fragment="single">MGASGKWIKSLILKKSDKVDQDKVGKSGKWKLWRSSSGEIGSGWKGFKGNYRGTGSDGSDTGSVVGSDVFSAAVATVVRAQPKDFKAVRQEWAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-10-08" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GNS3</accession>
    <name>A0AAD5GNS3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">M8C21_027151</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">AA19_3_7</strain>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006281">
        <property type="protein sequence ID" value="KAI7749757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000129">
        <property type="entry name" value="Leucine-rich repeat receptor-like kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000731">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000307">
        <property type="entry name" value="pollen receptor-like kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000095">
        <property type="entry name" value="protein STRUBBELIG-RECEPTOR FAMILY 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050994">
        <property type="entry name" value="At_inactive_RLKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48010">
        <property type="entry name" value="OS05G0588300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48010:SF59">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 15" id="KW-0732">Signal</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042116899" description="Protein kinase domain-containing protein" evidence="15">
        <location>
            <begin position="25"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="256"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="344"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="292"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAI7749757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="628" mass="69279" checksum="5982A2084A71CD4D" modified="2024-05-29" version="1">MKLCSPLLIHSILLIFTQFSPLFADLDSDKQALLAFSEAVPHGKKLNWSNATNICTSWLGVICASNRTHVSGLRLPSVGLTGPIPPNTLGKLKSLQVISLRLNRLTGSLPSDLLSLPSLHRLFLQHNNLSGNIPKSFPPRMNVLDLSFNSFTGQIPDSIQNLTRLISLKLQNNSLSGSIPNIKFSRLRNLNISNNHLNGSIPPSFQKFPYSSFIGNSLLCGRPLNRCSPILPPPDVATPPPTAATTQKKSSKKLPIWAIIAIAVGGGVVIILFGILLYFCCLRKRRRNRSDSETVLGKGKSSNDQGSDKLKEGYDSELHETDQNNNVVFFEGCLYSFDVEDLLRASAEMLGKGSFGTSYKAMLEDSVTVVVKRLKDVVAGKKEFEQHMEIVGRVDPHANVVPLRAYYYSKDEKLLVYDYISTGSLLNLLHGNRAASRTPVSWEARVKIALGTARGIAHIHAFGFVHGNIKSANVLINQDGEGCISDSGIAPIISFPASTSRYIAGYRAPEVLQIRKHSHKSDVYSYGVLLLEMLTGKQPXXXXXXXXXXXXXXXXXXXXEEWTAEVFDVELMKYQNIEEEMVQMLQIAMACVVQQPDNRPTMGEVVKMIEEVRVSESANRLANDEQSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GNU3</accession>
    <name>A0AAD5GNU3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_006910</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006847">
        <property type="protein sequence ID" value="KAI7747056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GNU3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009873">
        <property type="term" value="P:ethylene-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00018">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.730.10:FF:000001">
        <property type="entry name" value="Ethylene-responsive transcription factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.730.10">
        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001471">
        <property type="entry name" value="AP2/ERF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31677">
        <property type="entry name" value="AP2 DOMAIN CLASS TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31677:SF231">
        <property type="entry name" value="ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00847">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00367">
        <property type="entry name" value="ETHRSPELEMNT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00380">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51032">
        <property type="entry name" value="AP2_ERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0936">Ethylene signaling pathway</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0611">Plant defense</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="9">
        <location>
            <begin position="26"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="91"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="94"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7747056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="205" mass="22281" checksum="C53CE4B0AC2CC94D" modified="2024-05-29" version="1">MVMKNDTPKTVTASALQKAAAVTEVHYRGVRKRPWGRYAAEIRDPGTKTRVWLGTFDTAEAAARAYDAAAISFRGSKAKINFPSTLELVGVDGNNSRSSTVESSSRENERNVGTVERDLIQRVDDGGGGYRFFVNGNQQTVAVVPYAPPVMVYAQQYPIHFNGGYGGRAESESGSSVVEYVPRDTSSSKRELNLDLCLAPPTVEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GNX2</accession>
    <name>A0AAD5GNX2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SMP-LTD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_029412</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006938">
        <property type="protein sequence ID" value="KAI7746608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GNX2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd21669">
        <property type="entry name" value="SMP_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031468">
        <property type="entry name" value="SMP_LBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47261">
        <property type="entry name" value="CALCIUM-DEPENDENT LIPID-BINDING (CALB DOMAIN) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47261:SF2">
        <property type="entry name" value="CALCIUM-DEPENDENT LIPID-BINDING (CALB DOMAIN) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25669">
        <property type="entry name" value="SMP_MUG190-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51847">
        <property type="entry name" value="SMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="4" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SMP-LTD" evidence="6">
        <location>
            <begin position="171"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7746608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="396" mass="45101" checksum="D2206369896C3986" modified="2024-05-29" version="1" fragment="single">MVSCESSKVLWVSSSSSLNCPCNRVTQSSFSSIVRVYRRRKLSLIWASVVPNNGRKKNLKLRVSNELVEGDDDSLSESAIPLGSSNNFTNFTEDPIVDKLRTQLGVIHPIPSPPINRNIVGLFVFFFFVGVAFDKLWTSRKRNKPRDAGSPGMWPQVPTSFSLFLEKDLQRKESVEWVNMVLGKLWKVYRGGLENWVIGLLQPVIDNLQKPDYVERVEIKQFSLGEEPLSVRNVERRTSRRVNDLQYQIGLRYTGGARMLLMLSLKFGIIPIKVPVGIRDFDIDGELWVKLRLIPTEPWVGAVSWAFVSLPKIKFELSPFRLFNLMAIPVLSMFLTKLLTEDLPRLFVRPKKIVLDFQKGKTVGPVRNDFKTGEMQEGNKDFVGELSVTLVDARKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GPH7</accession>
    <name>A0AAD5GPH7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_029064</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ARG7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006126">
        <property type="protein sequence ID" value="KAI7750537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GPH7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009733">
        <property type="term" value="P:response to auxin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003676">
        <property type="entry name" value="SAUR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31929">
        <property type="entry name" value="SAUR-LIKE AUXIN-RESPONSIVE PROTEIN FAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02519">
        <property type="entry name" value="Auxin_inducible"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7750537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="99" mass="11372" checksum="DF3F31227BBC0128" modified="2024-05-29" version="1">MGVMKLFSSLISIVKGFSRLNAYRNRKCHWDVPKGHLAVYVGEQEKRRFVVPISCLEQPLFQKLLRESEEEFGFDHSMGGITLSCEEDAFFQLTSILNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GPL7</accession>
    <name>A0AAD5GPL7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ArsA/GET3 Anion-transporting ATPase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_002489</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the arsA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006644">
        <property type="protein sequence ID" value="KAI7747896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GPL7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043529">
        <property type="term" value="C:GET complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071816">
        <property type="term" value="P:tail-anchored membrane protein insertion into ER membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02035">
        <property type="entry name" value="ArsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025723">
        <property type="entry name" value="ArsA/GET3_ATPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016300">
        <property type="entry name" value="ATPase_ArsA/GET3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00345">
        <property type="entry name" value="GET3_arsA_TRC40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10803">
        <property type="entry name" value="ARSENICAL PUMP-DRIVING ATPASE ARSENITE-TRANSLOCATING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10803:SF3">
        <property type="entry name" value="ATPASE GET3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02374">
        <property type="entry name" value="ArsA_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ArsA/GET3 Anion-transporting ATPase-like" evidence="4">
        <location>
            <begin position="10"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="243"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7747896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="276" mass="31419" checksum="1B61345A8B994880" modified="2024-05-29" version="1" fragment="single">DEEVDPTVENEDTGNSDGMDGFLSDLANSIPGIDEAMSFAEMLKLVQTMDYSVIVFDTAPTGHTLRLLQFPSTLEKGLDKMMSLKSKFGGLLGQMTRMLGVGEEFGEDAILGKLEGMKDVIEQVNRQFKDPDLTTFVCVCIPEFLSLYETERLVQELTKFEIDTHNIIINQVLFDEEGIESKLLKARMKMQQKYLDQFYMLYDDFHITKLPLLPQEVCGVEALKAFSEHFLKPYQPSLTQGTIEELEQRVSRLQQQLKDAESELERARKGKQPIDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GQ84</accession>
    <name>A0AAD5GQ84_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Non-specific lipid-transfer protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_027912</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Plant non-specific lipid-transfer proteins transfer phospholipids as well as galactolipids across membranes. May play a role in wax or cutin deposition in the cell walls of expanding epidermal cells and certain secretory tissues.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the plant LTP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006619">
        <property type="protein sequence ID" value="KAI7747988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GQ84"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01960">
        <property type="entry name" value="nsLTP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.110.10">
        <property type="entry name" value="Plant lipid-transfer and hydrophobic proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036312">
        <property type="entry name" value="Bifun_inhib/LTP/seed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016140">
        <property type="entry name" value="Bifunc_inhib/LTP/seed_store"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000528">
        <property type="entry name" value="Plant_nsLTP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33076">
        <property type="entry name" value="NON-SPECIFIC LIPID-TRANSFER PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00234">
        <property type="entry name" value="Tryp_alpha_amyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00382">
        <property type="entry name" value="LIPIDTRNSFER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00499">
        <property type="entry name" value="AAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47699">
        <property type="entry name" value="Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00597">
        <property type="entry name" value="PLANT_LTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="2 4" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042174776" description="Non-specific lipid-transfer protein" evidence="5">
        <location>
            <begin position="26"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical" evidence="6">
        <location>
            <begin position="29"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7747988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="117" mass="12087" checksum="A448D36A9422477C" modified="2024-05-29" version="1">MASMLMKMACVVMAYMVVLAPHAEAAITCGKVVSSLLPCLGYLQSRGALTPACCNGVKSLNTAANNSADRKTACGCLKNAYKSNPGINQGNAASLPSKCGVNIPYKISPNTDCSKVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GQS8</accession>
    <name>A0AAD5GQS8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">65-kDa microtubule-associated protein 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_000097</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MAP65/ASE1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006501">
        <property type="protein sequence ID" value="KAI7748558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GQS8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1520:FF:000002">
        <property type="entry name" value="65-kDa microtubule-associated protein 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1520">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007145">
        <property type="entry name" value="MAP65_Ase1_PRC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19321:SF0">
        <property type="entry name" value="65-KDA MICROTUBULE-ASSOCIATED PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19321">
        <property type="entry name" value="PROTEIN REGULATOR OF CYTOKINESIS 1 PRC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03999">
        <property type="entry name" value="MAP65_ASE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="484"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="511"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="533"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7748558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="581" mass="66058" checksum="55593E17CF398F35" modified="2024-05-29" version="1">MMAVSPSTVRTATTCNTLLRELQQIWMDIGENEAEKDRMLMELERECLEVYRRKVDEAATAKARLHQAVAAKEAELATLIAALGDLNTHFPINIENKSSSLKAQLAMVTPLVEDLKVKKEERIKQFADIKSQIEKINGEISGFSQFVDSLNLEEQDLSLRKLGEYQSHLRTLQKEKSERLHKVLAHVNEVHSMCSVLGLDFGKTISDVHPSLHGTSLEQATNISDNTLEGLENAILKLKTEKKVRFQKLKDIAASLFELWQIMDSTREEKSYFSKITSVIRLSEAEIVEPAALSLEVIEQASQEVERLSKLKASRMKELVMKRRSELEAICLKTHIEPDPSTAIDKSNAMIDSAQISKVKDEALSRKEIMDRIERWLSACEEERWLEDYNLDQNRYSAGRGAHLSLKRAERARIAVNKIPTMVDNLISKTIAWENEKNKSFLYDGVRLVTILEEYKLTRLQKEEEKKRFRDQKKLQDLLLTERESIYGSKPSPRRASSFRMSNGHRGNGNGSMTPTPRRNSVASPTMEPMTPRSYSGRQNGYSKEMRRLTTAPLNFVAIPKEDTISFSSVYGSEPGSPLQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GRK5</accession>
    <name>A0AAD5GRK5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">M8C21_013031</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="22">AA19_3_7</strain>
            <tissue evidence="22">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010006093">
        <property type="protein sequence ID" value="KAI7750729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GRK5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000453">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase HSL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000387">
        <property type="entry name" value="Probable LRR receptor-like serine/threonine-protein kinase At1g06840"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000039">
        <property type="entry name" value="receptor-like protein kinase FERONIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45974:SF216">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45974">
        <property type="entry name" value="RECEPTOR-LIKE PROTEIN 55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="14 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-0675">Receptor</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="534"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="21">
        <location>
            <begin position="565"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="850"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="851"/>
            <end position="872"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="KAI7750729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="885" mass="99163" checksum="09646481F013DBCF" modified="2024-05-29" version="1">MMGFKFKSSGYVFAVAVYWLLMISVAKVTDPAEVSALLAVKGNLVDPMHHLDNWNKGDPCTSNWTGVICVHKSNVDKFWHVQEIQLLNMNLSGRLAPELGQFPHLKILDFMWNNLTGSIPKEIGNISSLVLLLLNGNRLTGSLPVELGYLTNLDRFQIDQNEISGTIPISFSNLENIKHIHFNNNSLTGQIPAELSNLSTLMHFQLDNNHFNGEIPSSYGNISELMKLSLRNCSLQGVLPDLSRIPNLSYIDLSKNRLTGSIPSNKLSNSMTTIDLSDNLLNGSIPESLSNLPFLQKLSLENNHLTGSISAHLWQNKSFNATSKLLLDFRNNSLSTVIGDLNPPVNASLRLHGNPICRNSSIQNKDQFCDREFEDFIRSISNNSTECPMQSCPIDNYFEYVPRSPTPCFCASPLRIDYRLKSPSFSYFPPYEDQFEVYVTSSLDLDFYQLKIDSITWERGPRLRMSLKLFPKVGTERSGIFSTSDVLRIRGTFTTWVFPGSHLFGPYELLDFTLVGPYAHLNAETPRKGIRRGVLITIVAVAVVCAILVSSILTVVIKKGQERYKHTLSRKALRGYGKVYKGKLWDNRTVAIKRAEEGSLQGEKEFLTEIEILSRLHHRNLVSLVGYCDEEQEQMLVYEFMPRGTLSDWLKAKSGESLSFRMRLQVALDSAKGILYLHTEANPPIFHRDIKSSNILLDSKFVAKVADFGLSRLAPSLDDNGAGPNYVSTLVRGTPGYLDPEYLLTHKLTDKSDVYSLGVVLLEILTGMRPISHGKNIVREVKIAHQEGIMFSIIDNKMGSYPSEWVEKFVSLALRCCNDKPEKRPSMSDVVHELEHILEKIPEMGVDFSEPESSSFVKTSSSFNSSSIVPGSDINSGGDSIFLPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-10-08" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GSC5</accession>
    <name>A0AAD5GSC5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Methyltransferase</fullName>
            <ecNumber evidence="11">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_032280</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Endomembrane system</location>
            <topology evidence="10">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 11">Membrane</location>
            <topology evidence="1 11">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the methyltransferase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="11"/>
    <dbReference type="EMBL" id="JAMZMK010005404">
        <property type="protein sequence ID" value="KAI7753537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000119">
        <property type="entry name" value="probable pectin methyltransferase QUA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004159">
        <property type="entry name" value="Put_SAM_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10108:SF1139">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10108">
        <property type="entry name" value="SAM-DEPENDENT METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03141">
        <property type="entry name" value="Methyltransf_29"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 11" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU366043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7753537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="573" mass="64319" checksum="E0DFB25CE17E27E8" modified="2024-05-29" version="1">MDVRIIPWLHKLQLFSGHRPPKNWLLLCVVSVFVLIALLSSSSTGGFTSFTDTVKPEVYTNYRRLKEHTASDYIELENVSSGSSEIKGIRLCGKEREDYVPCYNVSANVLVGYKDGEEFDRHCEVSQDEVFCLVRPPKDYKTPLSWPVGRDVIWNANVKISKDQFLSSGSMTKRLMLLEENQISFHSDDELIFDGVKDHSRQVAEMIGLGSDAEFWQAGVRTVLDIGCGFGSFGAHLLSLKLMAICMAAYELTGSQVQLSLERGLPAIIGNFISRKLPFPSLSYDMIHCAQCGVSWDQKDGMFLIEANRILKPGGYFVLHGTSLSTGKGSMIEEFTHKICWTLIGRQEETFIWXXXXPQCYSSSTQGVIPPCREEPEEIQSYYQPLASCVIGTASERWIPVQNKSSNSHITPAELGIYGKPGDEDPLPPYNMIRNVMDMNARFGGLNSVFLEAGKSAWVMNVVPFRARNTLPLILDQGFIGVLHDWCEPFPTYPRTYDMLHANGLLSHLISEGCSITNLLLEMDRILRPEGWVVLSDKLGPIEDARAVATQIRWEARVIDLENDSDQRLKLVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GX07</accession>
    <name>A0AAD5GX07_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_004306</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010001220">
        <property type="protein sequence ID" value="KAI7755366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GX07"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044296">
        <property type="entry name" value="HIPP46"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46371">
        <property type="entry name" value="OS04G0464100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7755366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="121" mass="12786" checksum="C05C610198AE5540" modified="2024-05-29" version="1" fragment="single">VVVHSFEAVDLISNNLIVLQQKIVVKMAANSEKKSRKVLKIAVSVAGVESVAFVGSDKDQITLTGEGIDSIEVAALLRKSVGYTELVRVGSVEEKKPATPASSVAVQTAAVPPQLLRSVVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GXT3</accession>
    <name>A0AAD5GXT3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_014390</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000216">
        <property type="protein sequence ID" value="KAI7756939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GXT3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051824">
        <property type="entry name" value="LRR_Rcpt-Like_S/T_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48006:SF82">
        <property type="entry name" value="CONCANAVALIN A-LIKE LECTIN_GLUCANASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48006">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN DDB_G0281931-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="53"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="144"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7756939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="439" mass="48410" checksum="CA536776FD1A7F2D" modified="2024-05-29" version="1">MQRGGVWYKGNCLSNVGEKDQRNASFCHNEALAVMIEPRRSGNDSTKSGKGKIVVASSVVCGVVVGGVALFGVVLVVVRSECFGGGCAATPQIKVLLEKVSPAYTVKMLTDARYISETMKLGAVGILAYRTYVLGELIEATNNFHESNRMGNGSHGQLYKGQLNDGTMFAIRNIKMKKRCTIQSLTRQLELISKLRHPNLVNPVGHCFDRQPDESAIIRGVFLVFEFVPNGTLREFLTAGQKLSWAQRLAAAIGISKGIQFLSTHITQRIFPNNLKITDVLLDQSFKVKISGYNLPLQAESKVAGHSVTKSSEIFSKRFEIEENDHDHVYDLGVIMLELILGRPINCINDTTIAKDMLQVSLVADEVARRSIVDPLVSKECCDESLKTLMELCVRCLSKDRPSIDDVLWNLEFIAQAHASSTEDSDHSNQMPTSIECIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BK14</accession>
    <name>A0AAD5BK14_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Transaldolase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_015458</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Transaldolase is important for the balance of metabolites in the pentose-phosphate pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>D-sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-erythrose 4-phosphate + beta-D-fructose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:17053"/>
            <dbReference type="ChEBI" id="CHEBI:16897"/>
            <dbReference type="ChEBI" id="CHEBI:57483"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="2.2.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Carbohydrate degradation; pentose phosphate pathway.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the transaldolase family. Type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012184">
        <property type="protein sequence ID" value="KAI7724597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BK14"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004801">
        <property type="term" value="F:transaldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006098">
        <property type="term" value="P:pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00955">
        <property type="entry name" value="Transaldolase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00493">
        <property type="entry name" value="Transaldolase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001585">
        <property type="entry name" value="TAL/FSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004732">
        <property type="entry name" value="Transaldolase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018225">
        <property type="entry name" value="Transaldolase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002881">
        <property type="entry name" value="PRK03343.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10683">
        <property type="entry name" value="TRANSALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10683:SF31">
        <property type="entry name" value="TRANSALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00923">
        <property type="entry name" value="TAL_FSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01054">
        <property type="entry name" value="TRANSALDOLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0570">Pentose shunt</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0704">Schiff base</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7724597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="431" mass="47230" checksum="1CAC96A58B4EA9ED" modified="2024-05-29" version="1" fragment="single">RQKQYRQLTSHLRKAHPSIGHRGSPLNTQKTKLHQLPLMASIITNISLPATPLNSSPVLLPPPTTTTFLLRFPSKPALSSSRLSLYSTLPLTASFLVKCSQNGGNGSHVKRTSLHDLYEKQGQSPWYDNLCRPVTDLIPLIESGVRGVTSNPAIFQKAISTSNAYNDQFRELVHGGKDIESAYWELVVKDIQDACRLFEPIYDETDGADGTVDAAKWLHKVVDRPNVHIKIPATAACVPSVKEVISLGISVNLIFSLSRYEAVIDAYLDGLEASGLNDLSRITSVASFYVSRVDTLVDSMLEKIGIPEALDLRGKAANAQAALAFQLYQKKFSGPRWEALVRKGAKKQRLLWASTNVKNPAYPDTLYVDPLVGPDTVSTMPDQALQAFIDHGTVGRTIDSNVSEAEGIYSAIEKLGINWGHVGSQLEFEGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BKM7</accession>
    <name>A0AAD5BKM7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cytochrome P450 94B3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_004033</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012089">
        <property type="protein sequence ID" value="KAI7725042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BKM7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11064">
        <property type="entry name" value="CYP86A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24296">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0349">Heme</keyword>
    <keyword evidence="5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="6">
        <location>
            <position position="358"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7725042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="416" mass="47489" checksum="F7A3E54E927090A1" modified="2024-05-29" version="1">MIVTANPENIEHILKTRFENYPKGKPFTDLLGDFLGIGIFNVDGELWSTQRKLASHEFSAKSLREFVVSVLEDEVTKRLVPLLENAVRDDEVVDMQDVLRRLAFDTICKISLGWDPCCLDYTRAVPPLVTAFDTAAGASAMRGTSSVSWVWKVKRLLNVGRERQLKEAVGVVHRAVDEIIRARREHMDGSDNNRDLLSRFMSSGHSDDLIRDMVISFLMAGRDTTSAAMTWLFWLLTRHPSIEKDVLKDVTFHDNKLTFEELKKMDYLKACLCESMRLYPPVVWDSKHAAEADVLPDGTAVYKGNRVMYFPYGMGRMETLWGKDCLEFRPDRWFSEPGGLKMESPYKFPVFQAGPRVCLGKEMAFIQMKYVMASVLSRFEFVSVSPEEPVFVPLLTAHMDGGFKVRVRRRSGFCEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BLB5</accession>
    <name>A0AAD5BLB5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_010014</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011938">
        <property type="protein sequence ID" value="KAI7725435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BLB5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27006">
        <property type="entry name" value="PROMASTIGOTE SURFACE ANTIGEN PROTEIN PSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27006:SF562">
        <property type="entry name" value="REPEAT TRANSMEMBRANE PROTEIN KINASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="127"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7725435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="149" mass="16420" checksum="F9A3DCDEC46A6F57" modified="2024-05-29" version="1" fragment="single">GYMAPEYALEGFFSTKSDVFSFGVVVLEIISGKKNTGAPKLRQNLSLLGYAWNLWKENTPFEFMDQRLLESSNSIEVLKCIIVGLLCVQEDPGDRPSMTNVVLMLAGDITSLPNPKQPAFVARKTMSSSSSSSSYKQDLTLTMTLPEGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BMA4</accession>
    <name>A0AAD5BMA4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">arsenate reductase (glutathione/glutaredoxin)</fullName>
            <ecNumber evidence="2">1.20.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_029802</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>[glutaredoxin]-dithiol + arsenate + glutathione + H(+) = glutathionyl-S-S-[glutaredoxin] + arsenite + H2O</text>
            <dbReference type="Rhea" id="RHEA:22016"/>
            <dbReference type="Rhea" id="RHEA-COMP:10729"/>
            <dbReference type="Rhea" id="RHEA-COMP:17668"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29242"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:48597"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:146199"/>
            <dbReference type="EC" id="1.20.4.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.20.4.1" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010012093">
        <property type="protein sequence ID" value="KAI7725031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008794">
        <property type="term" value="F:arsenate reductase (glutaredoxin) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.250.10:FF:000037">
        <property type="entry name" value="Dual-specificity phosphatase CDC25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10828:SF38">
        <property type="entry name" value="ARSENICAL-RESISTANCE PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10828">
        <property type="entry name" value="M-PHASE INDUCER PHOSPHATASE DUAL SPECIFICITY PHOSPHATASE CDC25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="4">
        <location>
            <begin position="63"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00051619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7725031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="176" mass="18979" checksum="C22C50A35842E17A" modified="2024-05-29" version="1" fragment="single">CMXXXXXXXXXXXVIWIVGQIELTSDSSILVAITGLISPLTTSLTMAKPISYVTGSELLSLKERSNVAIVDVRDDERSYDGHIAGSLHFSSDTFTDRLPDLVEVAKAQGKDTLVFHCALSQVRGPTCARRFADYLAEAKEDAGIKNIMVLERGYNGWEASGKPVCHCSGANCKGGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BPF8</accession>
    <name>A0AAD5BPF8_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_019815</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011759">
        <property type="protein sequence ID" value="KAI7726081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BPF8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31513">
        <property type="entry name" value="EPHRIN TYPE-B RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31513:SF12">
        <property type="entry name" value="GLYCINE-RICH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="537"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="565"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="605"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="648"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="460"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="463"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7726081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="692" mass="74448" checksum="5208B9BC9DC35FEF" modified="2024-05-29" version="1" fragment="single">TGSGIISASGGDGFGGGGGGRISADVVIVYGALRMTVKMFLMWKSQLLIDVEGDKNVQNSFLEASNLIVLKESSTIRSNANLGVHGQGLLNLSGPGDHIEAQRLVLSLFYGVNVGPGSVLRGPLENMTANAVVPKLNCESQQCPNELLNPPDDCNVNASLPFTLQICRVEDILVQGCIGGIGKGEVLSVGVGSGGGYGGSGGYGCYNGTCIDGGLPYGNADLPCWLGSGGGTDDITDPTAAGGGVLVIGSLDHPVSSLSVDGSLGADGGSHTIVEQDDWFQGSGDGGGGGGLREFVRSEYDHACLRSCRSRALYEGLKVAATSDLMLAYVDFFLGGDEKRSDLPPPLHQRFPLSLLFGGDGSYMAPFSLHSDNVITTLINQSVPPTTWYRFVAGLNAQLRLVRRGRLRVMFSAAVTWIETYANPTLKIYGIRVDLALFQATSGNNHQYGIVVTSVRVDPREQLPSNNDNAFQEDSREDKSLYQGQGDTNENLIQKKSYDGRVLDIDSVKSLKENRQIVSYALSFIIHNTKPVGHQDLVGLVISVLLLGDFSLVLLTLFQLYSFSLAHVFLVLFILPLGILLPFPAGINALFSQGPRRSSGLARIYALWNITSLVNVVVAFFCGYAHYSSQASKTPSELQPWTMEESEWWIFPVALVLCKCIQSIFINWHIANLEIQDRSLYSSDTDMFWQSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BQT3</accession>
    <name>A0AAD5BQT3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_027173</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011645">
        <property type="protein sequence ID" value="KAI7726514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BQT3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7726514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="138" mass="15711" checksum="442B724B269E7294" modified="2024-05-29" version="1">MSTLTLKKPYEDMTQPRGYDSAKRFLWAIKRIKTFRQAQITQAQMWLDIHSLRIFVAHQSALNLHTATVNYLGCGMMGSNVGFFFTKTCIIAIPYELLVLLDEVGFNHQNTFNNHAQMHIDAISGTPVVLKHELVEDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BRK9</accession>
    <name>A0AAD5BRK9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HVA22-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_024550</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011489">
        <property type="protein sequence ID" value="KAI7726919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BRK9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004345">
        <property type="entry name" value="TB2_DP1_HVA22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12300">
        <property type="entry name" value="HVA22-LIKE PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12300:SF117">
        <property type="entry name" value="LP05237P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03134">
        <property type="entry name" value="TB2_DP1_HVA22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="143"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="149"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="165"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="186"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="219"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="231"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="255"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU362006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7726919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="289" mass="32210" checksum="10AAD379E7C5C8B4" modified="2024-05-29" version="1">MIGSFLTRGLVMVFGYAYPAYECFKCVEKNKPDIEQLRFWCQYWILVAVLTVCERIGDTFISWVPMYSEAKLAFYIYLWFPKTKGTSYVYDSFFQPYISKHETEIDRNLSELRTMAGDMTVLYWQRAASYIQTRTLDIIQYVASQSTPKPRPSKPQPPVKARQPAPTNKGKTAAQQQETREPPSPAASSSSSSSSSNSGSDEIEIQSGAGKPQVPPVPAVAKAPKATPTQNPAVTTELQTTTEPEKKQKQIEPAGSSSLKASPSTVKEIMMEESGRVTRARLRKAISLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BS20</accession>
    <name>A0AAD5BS20_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_020423</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011384">
        <property type="protein sequence ID" value="KAI7727418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BS20"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7727418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="102" mass="11379" checksum="9ADA80B9166A4CF9" modified="2024-05-29" version="1">MKPKILEHQTFASLLVSRSKWNNKTLGKNGLDVDALHISHIQLNQAQSRDAGFIALMEESIVDSSFVIPCLEEVVITVRSPLNVEQKMSTLEALVKFGMVRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BSC4</accession>
    <name>A0AAD5BSC4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">M8C21_014576</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">AA19_3_7</strain>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011217">
        <property type="protein sequence ID" value="KAI7728414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BSC4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000307">
        <property type="entry name" value="pollen receptor-like kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000234">
        <property type="entry name" value="Probable inactive receptor kinase RLK902"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000095">
        <property type="entry name" value="protein STRUBBELIG-RECEPTOR FAMILY 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050994">
        <property type="entry name" value="At_inactive_RLKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48010">
        <property type="entry name" value="OS05G0588300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48010:SF59">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 14" id="KW-0732">Signal</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042198152" description="Protein kinase domain-containing protein" evidence="14">
        <location>
            <begin position="25"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="247"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="332"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="281"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="604"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="295"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="369"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAI7728414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="628" mass="68730" checksum="190660FB879F4785" modified="2024-05-29" version="1">MGLCSAQLVKSVLLIVFLLPLTYADLNSDKQALLAFANAVPHGRKLNWSNNTAICTSWVGINCSSDGTRVFALRLPAVGLKGPIPSNTLGKLDALQILSLRSNRLNGSLPTDLLSLPSLRYLYLQRNNFTTDIPASFPPRLIVLDLSFNSFTGNIPDSIQNLTQLTGLNLQNNSLSGPVPNITLSGLKQLNISYNHLNGSIPSSLQIFPSSAFIGNSFSCGLPLNPCSLPPSSPPVSPTKHSSKKKFPLWAIIAIAAGGGTALLLLVIIIIFCCMKKKSSDPSRVTKAKSSTGGRGDKPREEFGSGVQEPEKNKLVFFEGCSYNFDLEDLLRASAEVLGKGSFGTAYKAVLEESTTVVVKRLKEVVVGKKEFEQQMEIVGRVGQHQNVVPLRAYYYSKDEKLLVYDYYSAGSLLTLLHGNRGSERTAVDWETRVKIALGAARGIAHIHSVGGPKFTHGNIKSSNVLVTQDSIGCISDIGLAPLMNFPAPSSRHAVGYRAPEVLETRKHTHKSDVYSFGVLLLEMLTGKQPIQSPGREDMVDLPRWVQSVVREEWTAEVFDVELMKFQNIEEEMVQMLQIGMACVVRVPDMRPTMDEVVRMIEEVRASDSENRPSSDDNKSKDSRVQSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BSE8</accession>
    <name>A0AAD5BSE8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_006136</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011180">
        <property type="protein sequence ID" value="KAI7728632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BSE8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046959">
        <property type="entry name" value="PRK1-6/SRF4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48007">
        <property type="entry name" value="LEUCINE-RICH REPEAT RECEPTOR-LIKE PROTEIN KINASE PXC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48007:SF39">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="287"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="176"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="228"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="549"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="176"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="243"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="581"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7728632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="581" mass="62765" checksum="D8FD88C743B472EC" modified="2024-05-29" version="1" fragment="single">GGDKEALLGLKSSIDPSNNLQWSDNNFCKWEGVRECLRGRVSKLVLENMNLKGTLDSKNLNQLDQIRVLSFKQNSLIGQIPDLSGLINLKSLYLSSNNFSGDFPASLTTLHRLKTVVLSGNVPPLNQSGLKNLNLSNNKLSGQIPATPVLLKFNSTSFSGNVGLCGDAFNIPCSLSPTPSPSSSSSPANPSLTRPAGKSSHHRKIRNIVIIASSVVICLRKKSEKKSPEIGVKGSDTAAEAATGGGGGGVASGSGKNGGDDGGIGKLVFFSGGDTPEMSYSLEDLLKASAETLGRGTVGSTYKADARYPTVEEFRRHVDVIGRLRHQNLVPLRAYFQAKEERLLVYDYFPNGSLFSLIHGSRTSGGGKPLHWTSCLKIAEDLATGLLYIHQNPGLTHGNLKSSNVLLGSDFESCLTDYGLMSFRNPEFPEESSASSLFYRAPECRDPRRPITQPADVYSFGVLLLELLTGKTPFQDLVLEHGSDIPKWVKSVREEEIESGDEPTSSGNEASEEKLTALLNIAMACISLVPENRPVMKEVLRMIRETRAEGAHVSSNNSSDHSPGRWSDTVQSLPRDEHLSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BSP7</accession>
    <name>A0AAD5BSP7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein uL6c</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_013145</name>
        <name evidence="10" type="ORF">M8C21_013153</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the universal ribosomal protein uL6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011326">
        <property type="protein sequence ID" value="KAI7727755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAMZMK010011326">
        <property type="protein sequence ID" value="KAI7727763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BSP7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.930.12:FF:000001">
        <property type="entry name" value="50S ribosomal protein L6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.930.12:FF:000002">
        <property type="entry name" value="50S ribosomal protein L6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.930.12">
        <property type="entry name" value="Ribosomal protein L6, alpha-beta domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01365_B">
        <property type="entry name" value="Ribosomal_uL6_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000702">
        <property type="entry name" value="Ribosomal_uL6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036789">
        <property type="entry name" value="Ribosomal_uL6-like_a/b-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020040">
        <property type="entry name" value="Ribosomal_uL6_a/b-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019906">
        <property type="entry name" value="Ribosomal_uL6_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002358">
        <property type="entry name" value="Ribosomal_uL6_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03654">
        <property type="entry name" value="L6_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11655">
        <property type="entry name" value="60S/50S RIBOSOMAL PROTEIN L6/L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11655:SF14">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL6M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00347">
        <property type="entry name" value="Ribosomal_L6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00059">
        <property type="entry name" value="RIBOSOMALL6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56053">
        <property type="entry name" value="Ribosomal protein L6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00525">
        <property type="entry name" value="RIBOSOMAL_L6_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL6 alpha-beta" evidence="8">
        <location>
            <begin position="52"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL6 alpha-beta" evidence="8">
        <location>
            <begin position="131"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00069413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7727755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7727763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="221" mass="24423" checksum="CC96A24B40BA1286" modified="2024-05-29" version="1">MATSLHTSQLGSAFLGERAGICVSTVPVTRLGFRRKTVECKESRIGKQPIEVPSNVVITLEGQDMKVKGPLGELALTYPREIAIDRQEAILKVSKAVETRRANQMHGLFRTLTSNMVVGVSKGFEKKLQLVGVGYRATVEGKELILNLGFSHPVKMTIPDDLKVKVEDNTRITVSGYDKSSIGQFAATIRKWRPPEPYKGKGVKYADEIIRRKEGKAGKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BT29</accession>
    <name>A0AAD5BT29_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Malic enzyme</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_001125</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="6">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="6">Divalent metal cations. Prefers magnesium or manganese.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the malic enzymes family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011338">
        <property type="protein sequence ID" value="KAI7727669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BT29"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004473">
        <property type="term" value="F:malate dehydrogenase (decarboxylating) (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006108">
        <property type="term" value="P:malate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10380:FF:000002">
        <property type="entry name" value="Malic enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10380">
        <property type="entry name" value="Malic enzyme, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015884">
        <property type="entry name" value="Malic_enzyme_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012301">
        <property type="entry name" value="Malic_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037062">
        <property type="entry name" value="Malic_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012302">
        <property type="entry name" value="Malic_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001891">
        <property type="entry name" value="Malic_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010052">
        <property type="entry name" value="PRK13529.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23406">
        <property type="entry name" value="MALIC ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23406:SF76">
        <property type="entry name" value="NADP-DEPENDENT MALIC ENZYME 4, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00390">
        <property type="entry name" value="malic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03949">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000106">
        <property type="entry name" value="ME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00072">
        <property type="entry name" value="MALOXRDTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01274">
        <property type="entry name" value="malic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00919">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00331">
        <property type="entry name" value="MALIC_ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Malic enzyme N-terminal" evidence="10">
        <location>
            <begin position="174"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="Malic enzyme NAD-binding" evidence="9">
        <location>
            <begin position="381"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="4">
        <location>
            <position position="197"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="284"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="266"/>
        </location>
        <ligand>
            <name>(S)-malate</name>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="356"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="357"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="380"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="437"/>
        </location>
        <ligand>
            <name>(S)-malate</name>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="481"/>
        </location>
        <ligand>
            <name>(S)-malate</name>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000106-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000106-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000106-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7727669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="599" mass="66146" checksum="EE9B03AD6F414AB5" modified="2024-05-29" version="1" fragment="single">KNSISGVSRTQSQSLRLSARRPVVVSMLNNNNNNNNNNNNNNNNRTERNVTVSVESAVKEVNGPVAVEVDESEGKKPTAAVGGGVEDVYGEDSATEDQSITPWFVSVASGYSLLRDPHHNKGLAFTEKERDAHYLRGLLPPVVVNHDLQVKKMMHNIRQYQVPLQRYQAMMDLQERNERLFYKLLIENVEELLPIVYTPTVGEACQKYGSIFKNPQGLFISLRDKQVPITIIFSCILGNLLGKILEILKNWPQKKIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEKMLNDEFYIGLRQRRASGQEYAELMNEFMTAVKQNYGEKVLIQFEDFANHNAFDLLEKYRTTHLVFNDDIQGTASVVLAGLISALKLTIRPTVLIGSSGAGQTFTKEVVETMSSFNEKPIILALSNPTSQSECTAEQAYTWSEGRAIFASGSPFDPVEYNGKVHVSGQANNAYIFPGFGLGLIISGAIRVHDDMLLAAYRAANELAEPALASARARTEALAEQVTHEHFDKGLIYPPFTNIRKISAHIAAKVAAKAYELGLASRLPQPENLVAYAESCMYSPKYRSYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BTA8</accession>
    <name>A0AAD5BTA8_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_014630</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011033">
        <property type="protein sequence ID" value="KAI7729241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BTA8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010333">
        <property type="term" value="F:terpene synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016102">
        <property type="term" value="P:diterpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046246">
        <property type="term" value="P:terpene biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00684">
        <property type="entry name" value="Terpene_cyclase_plant_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.600.10:FF:000007">
        <property type="entry name" value="Isoprene synthase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034741">
        <property type="entry name" value="Terpene_cyclase-like_1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044814">
        <property type="entry name" value="Terpene_cyclase_plant_C1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001906">
        <property type="entry name" value="Terpene_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050148">
        <property type="entry name" value="Terpene_synthase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005630">
        <property type="entry name" value="Terpene_synthase_metal-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31225">
        <property type="entry name" value="OS04G0344100 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31225:SF252">
        <property type="entry name" value="TERPENE SYNTHASE 12-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01397">
        <property type="entry name" value="Terpene_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03936">
        <property type="entry name" value="Terpene_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01019">
        <property type="entry name" value="Terpene_Cyclase_Like_1_C_Termi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="domain" description="Terpene synthase N-terminal" evidence="5">
        <location>
            <begin position="25"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="Terpene synthase metal-binding" evidence="6">
        <location>
            <begin position="203"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7729241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="508" mass="58648" checksum="DFC2AF5AE5BF1521" modified="2024-05-29" version="1">MSRVYVAYAAYIIILLSFDGPQINFMGKLRELEKKVKVLNVNFENGSFSTLELLEHIDDIERLGLGYRFQNHIRRLLDVITLIYGDVELEETEDSLHKASLRFRILRQHGYNVSQDFLKRFKDNRGGFIECIQTDVKGLLSLYEASHLAFMEESDLQEAKLFATEHLLKLKCLDGEVLENINRALEFPLYHRMLRLQASWWNDIGLSQKLGFVRDRLMECFFWTVGMVFEPQYHSCRVGLTKVGTLITVIDDIYDVYGSLDELETFTDAVKRWDINAMENMPKYVQLGFGALYHTITEMGSSSSVAPVEDITPILVKVWGELLETFLVEAKWTHDKLYIPTLEEYLDNAWRSVSGVVILTHGYFLINQEINKDVVETLEKYHDLMKWSSMVFRLYNDLATSSNEMEGGKNVNAISCYMHENGVCEEVARAYIKTLIDEAWRKLIKVHVACSQEFANPFIDMAINLARISCCTYQYGDGHGAPDARAKDRVLSVIIKPITIREKEHKYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BTV8</accession>
    <name>A0AAD5BTV8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_024332</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011008">
        <property type="protein sequence ID" value="KAI7729395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BTV8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030014">
        <property type="term" value="C:CCR4-NOT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16618">
        <property type="entry name" value="mRING-HC-C4C4_CNOT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039780">
        <property type="entry name" value="Mot2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039515">
        <property type="entry name" value="NOT4_mRING-HC-C4C4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12603">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12603:SF36">
        <property type="entry name" value="RNA BINDING (RRM_RBD_RNP MOTIFS) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14570">
        <property type="entry name" value="zf-RING_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="2">
        <location>
            <begin position="9"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7729395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="162" mass="18212" checksum="85E4454C26AB3C39" modified="2024-05-29" version="1">MSDEGDKTCPLCAEVMDLTDQQLRPCKCGYDICVWCWNHIMGMAEKDGTEGRCPACRTPYNKEKIVQTSSKCERLVAELSVEKKQKSQKGKTKTSEARKQLSSVRVIQRNLVYIVGLPLNLADEDILQRKEYFGQYGKVLKVSISRTAAGTIQHFANNTCSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BV47</accession>
    <name>A0AAD5BV47_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_019971</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RUS1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010852">
        <property type="protein sequence ID" value="KAI7730020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BV47"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006968">
        <property type="entry name" value="RUS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055412">
        <property type="entry name" value="UVB_sens_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054549">
        <property type="entry name" value="UVB_sens_RUS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12770:SF27">
        <property type="entry name" value="PROTEIN ROOT UVB SENSITIVE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12770">
        <property type="entry name" value="RUS1 FAMILY PROTEIN C16ORF58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24160">
        <property type="entry name" value="UVB_sens_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04884">
        <property type="entry name" value="UVB_sens_prot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein root UVB sensitive/RUS" evidence="3">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="Root UVB sensitive protein C-terminal" evidence="4">
        <location>
            <begin position="154"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="249"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF24160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7730020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="303" mass="33783" checksum="5E97A5A683DB9049" modified="2024-05-29" version="1">MYADFIGSAGSIFDLCTPLYPAYFLPLASLGNLTKAVARGLKDPSFRVIQSHFAVSGNLGDVAAKKQRTMVLSLANDESVTPSATSEINRLVASNAGLEGLIHKKEEVWEVSAQLVGLGLGILVLSTPGLVNSYPVNLKRARILVNMHVSSSAVLGCKECNKMENILSWEKFSVPRIVFGVPLSDMLGGDTSGLKVKKLLELYSKEKYILVLNQQPFKDFEILVSFKDGATSLSVLRSVWQAYWLYKNQSRQENMFDQLQESLKELDHQFHKFLQQLIDVGWDINSINLKVNKDVLIEDFSMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BYS6</accession>
    <name>A0AAD5BYS6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_019042</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010290">
        <property type="protein sequence ID" value="KAI7732181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BYS6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01958">
        <property type="entry name" value="HPS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.110.10">
        <property type="entry name" value="Plant lipid-transfer and hydrophobic proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036312">
        <property type="entry name" value="Bifun_inhib/LTP/seed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016140">
        <property type="entry name" value="Bifunc_inhib/LTP/seed_store"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027923">
        <property type="entry name" value="Hydrophob_seed_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051636">
        <property type="entry name" value="Plant_LTP/defense-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31731">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14547">
        <property type="entry name" value="Hydrophob_seed"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00499">
        <property type="entry name" value="AAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47699">
        <property type="entry name" value="Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042160894" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein" evidence="1">
        <location>
            <begin position="25"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical" evidence="2">
        <location>
            <begin position="37"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7732181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="120" mass="12417" checksum="AF292260DD83A8E8" modified="2024-05-29" version="1">MNPSKAFTVAFILANVILFTTVSANNPPCAPKTPTKCPKDTLKFGVCGDWLGLAHEVVGVQPDSKCCTVVKGLADLEAAVCLCTALKANVLGALKVEVPVALSLVVNSCGKKVPEGFKCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C247</accession>
    <name>A0AAD5C247_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SAM-dependent methyltransferase TRM5/TYW2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_023206</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010186">
        <property type="protein sequence ID" value="KAI7732651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C247"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008175">
        <property type="term" value="F:tRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002939">
        <property type="term" value="P:tRNA N1-guanine methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.110">
        <property type="entry name" value="Met-10+ protein-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030382">
        <property type="entry name" value="MeTrfase_TRM5/TYW2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056743">
        <property type="entry name" value="TRM5-TYW2-like_MTfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23245:SF36">
        <property type="entry name" value="TRNA (GUANINE(37)-N1)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23245">
        <property type="entry name" value="TRNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02475">
        <property type="entry name" value="TRM5-TYW2_MTfase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51684">
        <property type="entry name" value="SAM_MT_TRM5_TYW2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="SAM-dependent methyltransferase TRM5/TYW2-type" evidence="6">
        <location>
            <begin position="95"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7732651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="354" mass="40325" checksum="70740A32F5C1AF9E" modified="2024-05-29" version="1">MLLDESKFDLHLKLWALRIPRELCKAATKLLNGHMLDRPRIKPITEDPSSEKTRFVILSEKVQNPDLSDIPTQKLNELEKLCKIETVPYTLTLGYSYWGADHVLKQVLPPGLEVPSSFETIVKYFKNHPRIQTVVNKVGSIANEFRVPKFEILAGKPDMATEVKQYGATFKLDYGLVYWNSRLEHEHIRLVNKFNQGDIICDMFAGIGPFAIPAAQKGCLVYANDLNPDSIRYLKINADINKVNNNIHAYNMDARVFMSQLMTVPNGESEVVDSSLGSSDTCNIQENMKANTKERNSEMFQNKENKDEAESLLNAKIHEPVFHRVRDVAPNKAMYCFSFRLPEEACIDEEITNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C2D7</accession>
    <name>A0AAD5C2D7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Digalactosyldiacylglycerol synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_007030</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 4 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009839">
        <property type="protein sequence ID" value="KAI7734086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C2D7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009707">
        <property type="term" value="C:chloroplast outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046481">
        <property type="term" value="F:digalactosyldiacylglycerol synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019375">
        <property type="term" value="P:galactolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044525">
        <property type="entry name" value="DGDG1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46132:SF6">
        <property type="entry name" value="DIGALACTOSYLDIACYLGLYCEROL SYNTHASE 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46132">
        <property type="entry name" value="DIGALACTOSYLDIACYLGLYCEROL SYNTHASE 2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0934">Plastid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7734086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="124" mass="13713" checksum="11913CB9A099D1EF" modified="2024-05-29" version="1">MFSIQVLRLSAATQDLPKSVICNVHGVNPKFLEIGERMAAADKEEGGDQKFSKGAYFLGKMVWAKGYRELIELLAKQKQDLNGFKLDVYGNGEDAHEVQSAAKSLDLNVNFMKGRDHADDSLHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C318</accession>
    <name>A0AAD5C318_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Magnesium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_013936</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Magnesium transporter that may mediate the influx of magnesium.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the CorA metal ion transporter (MIT) (TC 1.A.35.5) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009890">
        <property type="protein sequence ID" value="KAI7733880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C318"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12823">
        <property type="entry name" value="Mrs2_Mfm1p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.128.330:FF:000001">
        <property type="entry name" value="Magnesium transporter MRS2-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.340">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039204">
        <property type="entry name" value="MRS2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13890:SF35">
        <property type="entry name" value="MAGNESIUM TRANSPORTER MRS2-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13890">
        <property type="entry name" value="RNA SPLICING PROTEIN MRS2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22099">
        <property type="entry name" value="MRS2-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0406">Ion transport</keyword>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="421"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="464"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="160"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="325"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="160"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="180"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="326"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="338"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="355"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU366041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7733880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="493" mass="55069" checksum="497A5337E8FEB1E7" modified="2024-05-29" version="1">MTPPPLSTTLEQQQQQHTFETIRTTTTTTPLTINAPPLRKKSAATRPWLLLDSTGQAQVIEAGKHATMRRTGLPARDLRILDPILSYPSTVLGRERAIVINLEHVKAIITAQEVLLLNSKDPSVTPFVGELQRRILRHHHATVSQKEMVDGDTDWSHLYDLGEPRSREASPPRLATEGSQEVKADGKHSFDKRDGPKLLPFEFVALEACLEAACSSLDNEAKTLEQEAHPALDKLTSKISTLNLERVRQIKSRLVAITGRVQKVRDELEQLLDDDGDMAEMYLTDKLEQQLDNSSSSSSFVNEKDALNEEVLQTEIADRVLGESLTDGNENTNAQEQMDNHSEHLFRVGRESRGTRTSTTKSSSGKHLDIEELEMLLEAYFVQIDGTLNKLSTLREYVDDTEDYINIMLDDKQNHLLQMGVMLTTATLVVSAFVVVAGMFGMNIEIELFRADTPEQKKVGNGKFLWTVGGGTTGTIILYVIAIVWCKSKRLLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C4F7</accession>
    <name>A0AAD5C4F7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Maf-like protein DDB_G0281937</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_003982</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009625">
        <property type="protein sequence ID" value="KAI7734895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C4F7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047429">
        <property type="term" value="F:nucleoside triphosphate diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.950.10:FF:000008">
        <property type="entry name" value="Maf-like protein, expressed"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00528">
        <property type="entry name" value="Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029001">
        <property type="entry name" value="ITPase-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003697">
        <property type="entry name" value="Maf-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43213">
        <property type="entry name" value="BIFUNCTIONAL DTTP/UTP PYROPHOSPHATASE/METHYLTRANSFERASE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43213:SF14">
        <property type="entry name" value="MAF-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02545">
        <property type="entry name" value="Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006305">
        <property type="entry name" value="Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52972">
        <property type="entry name" value="ITPase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7734895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="206" mass="22484" checksum="83E7219B78354824" modified="2024-05-29" version="1">MEPPKSPFKIILGSSSVARQKILAEMGYEFTIMSADIDEKAIRTEKPEDLVMALAKAKADAIVSKLQTSDDQDKDAKPSILIASDTVVVYEGAVREKPSGEEEARQFIKSYSGKHAATLSSVFVTNLKTGFTKGDWDKVEIHFHEISDDVIDKLLEEGLVLRVAGALIIEHPLIVPCVKDVKGTTDSVMGLPKALTEKLLKEAMNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C4H2</accession>
    <name>A0AAD5C4H2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_003390</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009893">
        <property type="protein sequence ID" value="KAI7733866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C4H2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7733866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="70" mass="8470" checksum="DE919110B0EDABBD" modified="2024-05-29" version="1">MALRPNSSSLMALKTVDEHNKKHGSYFLNEHNKRQVDYRHVQKLFLFFSSRDSNHKQWVVHLISKFWLRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C521</accession>
    <name>A0AAD5C521_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_025086</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ARPC5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009694">
        <property type="protein sequence ID" value="KAI7734558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C521"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005885">
        <property type="term" value="C:Arp2/3 protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034314">
        <property type="term" value="P:Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030833">
        <property type="term" value="P:regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006789">
        <property type="entry name" value="ARPC5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036743">
        <property type="entry name" value="ARPC5_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12644">
        <property type="entry name" value="ARP2/3 COMPLEX 16 KD SUBUNIT P16-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69103">
        <property type="entry name" value="Arp2/3 complex 16 kDa subunit ARPC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7734558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="62" mass="7119" checksum="E852E7D0E4B12084" modified="2024-05-29" version="1" fragment="single">MGEHVEADNAEAIITRIEHKSRKIESLLKQYKPVEALKTALEGSPPKTRDERCKFINLLMIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C6D9</accession>
    <name>A0AAD5C6D9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_030412</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009383">
        <property type="protein sequence ID" value="KAI7736067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C6D9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044296">
        <property type="entry name" value="HIPP46"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46371">
        <property type="entry name" value="OS04G0464100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7736067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="114" mass="12836" checksum="AAADBB309A5E7663" modified="2024-05-29" version="1">MGKQKIVVKVSTNDEKKARKAMQIAVSISGVESASFVGSNKDQIAVTGEGIDSFELTTLLRKRIGYTELVSVGPVEEKKPESKETKGSKPVVKVDPYEYYYYNYGMPYYYVNQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C6X1</accession>
    <name>A0AAD5C6X1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_018317</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009275">
        <property type="protein sequence ID" value="KAI7736441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C6X1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7736441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="95" mass="10537" checksum="28D8275D1771A4BA" modified="2024-05-29" version="1" fragment="single">SGQLIWGCVNGVKTTNICGVTAAKTYLLGLNAGLFRARRLLRTADDGIHKFSKAFEMMGTLHKSTIRYHMERGLNLDSLRFVGEIAKKEVIARAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C7J2</accession>
    <name>A0AAD5C7J2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_012646</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009194">
        <property type="protein sequence ID" value="KAI7736771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C7J2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7736771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="118" mass="13298" checksum="6A608DC6FDB7ED84" modified="2024-05-29" version="1">MIKSCSTPHLSPTAFSTPMYLASSCPNSGYSMYYGGNGGGGCVLAKGEVDGGSYVGFSLTLDFLRFKVYERHRWWRQSVKEEDDFHRIRIRKIKENDELHDSIVSILKLSPSTKRDKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C873</accession>
    <name>A0AAD5C873_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Short-chain dehydrogenase TIC 32, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_015413</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009439">
        <property type="protein sequence ID" value="KAI7735756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C873"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05327">
        <property type="entry name" value="retinol-DH_like_SDR_c_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055280">
        <property type="entry name" value="TIC32"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48476">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE TIC 32, CHLOROPLASTIC-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48476:SF1">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE TIC 32, CHLOROPLASTIC-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7735756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="317" mass="34348" checksum="374204825CAB2871" modified="2024-05-29" version="1">MFGLKGPSGFSRFSTAEEVTHGIDGSGLTAIVTGASSGIGTETARVLALRGVHVVMAIRNMSAGREVKEAILKQNPAAKIDAMELDVSSIASVNKFALDYKATGLPLNLLINNAGILATPYTLSEDNIELQFATNHVGHFHLTNLLLETMKRTAHKSSKEGRIVNVSSRRHRFCYPEGIRFDQINSQSGYNALSAYGQSKLANVLHANELARRLKDDGADITANSVHPGAIPTNIFRHHIFFSGLANIFGKFVLKNVQQGAATTCYVALHPQVKGISGKYFSNCNLDAASSQANDAQLAKKLWTFTENLIKENHPED</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C8G4</accession>
    <name>A0AAD5C8G4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Xyloglucan endotransglucosylase/hydrolase</fullName>
            <ecNumber evidence="7">2.4.1.207</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_007374</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Secreted</location>
            <location evidence="7">Cell wall</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Secreted</location>
            <location evidence="7">Extracellular space</location>
            <location evidence="7">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="7">Contains at least one intrachain disulfide bond essential for its enzymatic activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the glycosyl hydrolase 16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.207" evidence="7"/>
    <dbReference type="EMBL" id="JAMZMK010009072">
        <property type="protein sequence ID" value="KAI7737281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C8G4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016762">
        <property type="term" value="F:xyloglucan:xyloglucosyl transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042546">
        <property type="term" value="P:cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010411">
        <property type="term" value="P:xyloglucan metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02176">
        <property type="entry name" value="GH16_XET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000025">
        <property type="entry name" value="Xyloglucan endotransglucosylase/hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000757">
        <property type="entry name" value="Beta-glucanase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044791">
        <property type="entry name" value="Beta-glucanase/XTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008264">
        <property type="entry name" value="Beta_glucanase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010713">
        <property type="entry name" value="XET_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016455">
        <property type="entry name" value="XTH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31062">
        <property type="entry name" value="XYLOGLUCAN ENDOTRANSGLUCOSYLASE/HYDROLASE PROTEIN 8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00722">
        <property type="entry name" value="Glyco_hydro_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06955">
        <property type="entry name" value="XET_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005604">
        <property type="entry name" value="XET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00737">
        <property type="entry name" value="GLHYDRLASE16"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51762">
        <property type="entry name" value="GH16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0052">Apoplast</keyword>
    <keyword evidence="7" id="KW-0134">Cell wall</keyword>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="1 7" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041765426" description="Xyloglucan endotransglucosylase/hydrolase" evidence="7">
        <location>
            <begin position="26"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="GH16" evidence="8">
        <location>
            <begin position="20"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="101"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="105"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="6">
        <location>
            <position position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR005604-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR005604-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7737281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="292" mass="33173" checksum="A604008719608672" modified="2024-05-29" version="1">MAISSSSSFFVIALFLSLLVISTNATAKFEELFHPYWAADHFSFDGDAVNMKLDNFSGAGFSSKSKYMFGKVNVQIKLVEGDSAGTVTAFYMSSDGPKHHEFDFEFLGNTTGEPYIVQTNVYVNGVGNREQRLNLWFDPTKDFHTYSILWNQRQVVFLVDETPIRVHTNLEHKGIPFPRDQAMGVYSSIWNADDWATQGGRVKTDWTHAPFVASYRAFEIDGCECPVTTTQSDNAKRCANSGGWWDQPVMSELNLHQSHQLIWVRANHMIYDYCSDTGRFPSVPVECEHHRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C940</accession>
    <name>A0AAD5C940_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">serine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Seryl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_020324</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.11" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010009052">
        <property type="protein sequence ID" value="KAI7737377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C940"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004828">
        <property type="term" value="F:serine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006434">
        <property type="term" value="P:seryl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00770">
        <property type="entry name" value="SerRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000026">
        <property type="entry name" value="Seryl-tRNA synthetase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.40">
        <property type="entry name" value="Serine-tRNA synthetase, tRNA binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002317">
        <property type="entry name" value="Ser-tRNA-ligase_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015866">
        <property type="entry name" value="Ser-tRNA-synth_1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042103">
        <property type="entry name" value="SerRS_1_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033729">
        <property type="entry name" value="SerRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010978">
        <property type="entry name" value="tRNA-bd_arm"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00414">
        <property type="entry name" value="serS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11778">
        <property type="entry name" value="SERYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02403">
        <property type="entry name" value="Seryl_tRNA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001529">
        <property type="entry name" value="Ser-tRNA-synth_IIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00981">
        <property type="entry name" value="TRNASYNTHSER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46589">
        <property type="entry name" value="tRNA-binding arm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="12">
        <location>
            <begin position="97"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="4"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="221"/>
            <end position="223"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="237"/>
            <end position="240"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="244"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="310"/>
            <end position="313"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="346"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for serine binding" evidence="9">
        <location>
            <position position="348"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001529-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001529-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7737377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="393" mass="44565" checksum="75AE58D59684BBAC" modified="2024-05-29" version="1" fragment="single">QFELEQLRKDFNKINKEVAKLRISGGDASALIKNTEENKDSTAKKQAEVQEAYTALCSKLETVGNLVHDSVPVSDDEANNAVVRTWGERREEAKLKNHVELVELLGIADLKKGANVAGGRGYYLKGDGVLLNQALINFGLAFLGNRKYTPLQTPFFMRKDIMGKCAQLAQFDEELYKVTGEGDDKYLIATAEQPLCAYHLEDWIHPTELPLRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCLTKPTANESWEMHEEMIKNSEEFYQMLKLPYQVVSIVSGALNDAAAKKYDLEAWFPASKTYRELVSCSNCTDYQSRKLEIRYGQKKGNDQTKQYCHLLNSTLTATERTLCCILENYQTEKGVAIPEVLQPFMGGKTFLDFLKKIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C9L3</accession>
    <name>A0AAD5C9L3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_000963</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="6">Component of the mitochondrial ribosome small subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the universal ribosomal protein uS8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008930">
        <property type="protein sequence ID" value="KAI7737868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C9L3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.30:FF:000001">
        <property type="entry name" value="40S ribosomal protein S15a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.10:FF:000003">
        <property type="entry name" value="40S ribosomal protein S15a-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000630">
        <property type="entry name" value="Ribosomal_uS8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047863">
        <property type="entry name" value="Ribosomal_uS8_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035987">
        <property type="entry name" value="Ribosomal_uS8_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003115">
        <property type="entry name" value="PRK04034.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11758">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S15A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00410">
        <property type="entry name" value="Ribosomal_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56047">
        <property type="entry name" value="Ribosomal protein S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00053">
        <property type="entry name" value="RIBOSOMAL_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 7" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00063721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7737868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="129" mass="14861" checksum="4D9C90A444298F46" modified="2024-05-29" version="1">MGRRILNDALRTMVNAEKRGFASAQLQPISNVISSFLTIMKQRGYVKNFEVHDPHRVGKIQVELMGRIKDCRALTYRQDIKAHEIENYRLRTLPTQQWGYVVITTPNGVLDHEEAIKQNVGGQVLGYFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CBA9</accession>
    <name>A0AAD5CBA9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_011724</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008714">
        <property type="protein sequence ID" value="KAI7738843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CBA9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052306">
        <property type="entry name" value="CYP450_71D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47953:SF16">
        <property type="entry name" value="CYTOCHROME P450 71D8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47953">
        <property type="entry name" value="OS08G0105600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0349">Heme</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7738843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="194" mass="22348" checksum="06EDE2D91F5B4DBF" modified="2024-05-29" version="1">MRKICILELLSAKKVRSFESVRDQESWNLVETMVNIEGAVAIILTKKIFTMMNVVVSRVAVGTKCKDQAMLIELIKQIEVLSGGFDVFDLFPSFKVLHLNMFIAGTDTSSVITEWAMFELMINPRVMHKLQGEIRRVLNGKKKVYESDIKDLDYLRLMINGTLRLHPHVPLIPREYRAKCEICGYNHQCMENRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CDJ4</accession>
    <name>A0AAD5CDJ4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_029338</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ARG7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008543">
        <property type="protein sequence ID" value="KAI7739936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CDJ4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009733">
        <property type="term" value="P:response to auxin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003676">
        <property type="entry name" value="SAUR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31929">
        <property type="entry name" value="SAUR-LIKE AUXIN-RESPONSIVE PROTEIN FAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02519">
        <property type="entry name" value="Auxin_inducible"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7739936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="93" mass="10662" checksum="FA481ECB691BDB0F" modified="2024-05-29" version="1">MYTKLALGRYQSSSHVIDVPKGHFSVYVGENNLKRFIVPLAYLKHPSFQTLLNLAKDEFGYAHPMGCLTFPCKEETCLEHTENIRLTSLWSDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CDW9</accession>
    <name>A0AAD5CDW9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_010334</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008511">
        <property type="protein sequence ID" value="KAI7740116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CDW9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042000676" evidence="1">
        <location>
            <begin position="22"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7740116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="131" mass="14844" checksum="E4A3EAEEDFA40835" modified="2024-05-29" version="1" fragment="single">LLITGLRAALHLLCSFEQGHTEPEQQLQACPASNCSVIATLHWLYIAQVLDKLEYIGCYLMTKRLQFTILKVPNPKDMIPNSRTHMLNIASNRRGFPNMSNLDYGRKLYVDSIGNTLRLRLSQMGSYQGTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CFB8</accession>
    <name>A0AAD5CFB8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein RAE1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_006011</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008293">
        <property type="protein sequence ID" value="KAI7741018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CFB8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000146">
        <property type="entry name" value="protein RAE1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10971">
        <property type="entry name" value="MRNA EXPORT FACTOR AND BUB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="2"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="49"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="91"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="230"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7741018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="330" mass="36765" checksum="2C15955BC4F77BEE" modified="2024-05-29" version="1" fragment="single">VVSPPSDSVSSLSFSPKANYLVATSWDNQVRCWEITKSGTTVGSVGKASIAHDQPVLCSTWKDDGTTVFSGGCDKQVKMWPLLSGGQPVTVAMHDAPVKEVAWIPEMNLLVSGSWDKTLRYWDLRQANPVHTQQLPERCYALTVRHPLMVVGTADRNLIVFNLQNPQTEFKRITSPLKYQTRCVAAFPDQQGFLVGSIEGRVGVHHLDDQQQSKNFTFKCHREGNDIYSVNSLNFHPVHHTFATAGSDGAFNFWDKDSKQRLKAMSRCNQPIPCSSFNNDGSIYAYAVCYDWSKGAENHNPATAKTYIYLHLPQESEVKGKPRIGTSGRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CH24</accession>
    <name>A0AAD5CH24_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF3700 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_033931</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008350">
        <property type="protein sequence ID" value="KAI7740750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CH24"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024286">
        <property type="entry name" value="DUF3700"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044828">
        <property type="entry name" value="TSJT1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45952">
        <property type="entry name" value="ALUMINUM INDUCED PROTEIN WITH YGL AND LRDR MOTIFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45952:SF5">
        <property type="entry name" value="ALUMINUM INDUCED PROTEIN WITH YGL AND LRDR MOTIFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12481">
        <property type="entry name" value="DUF3700"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01172">
        <property type="entry name" value="DUF3700"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF3700" evidence="1">
        <location>
            <begin position="2"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7740750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="237" mass="25521" checksum="EB40EC20F1AFBDAE" modified="2024-05-29" version="1">MLGVFSSSIVSPPEELVAAGSRTPSPKITAKSLVNRFVGNNALAVSMQIGDDVQLAYTHQNESITHPRSFAVKDEIFCLFEGTLDNLGSLKQQYGLSKSANEVVLVIEAYRALRDRAPYPINQVVGHLSGNFSFVVFDKSTSTVFVATDQHGKVPLYWGITADGCVAFANDADLLKGACGKSLATFPQGCFYSTAIGELRCYENPKNKITAVPATEEEIWGAKFMVEGPSSIISATQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CI63</accession>
    <name>A0AAD5CI63_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">[histone H3]-lysine(4) N-trimethyltransferase</fullName>
            <ecNumber evidence="2">2.1.1.354</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_021618</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-lysyl(4)-[histone H3] + 3 S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl(4)-[histone H3] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:60260"/>
            <dbReference type="Rhea" id="RHEA-COMP:15537"/>
            <dbReference type="Rhea" id="RHEA-COMP:15547"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="EC" id="2.1.1.354"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N(6),N(6)-dimethyl-L-lysyl(4)-[histone H3] + S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl(4)-[histone H3] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:60272"/>
            <dbReference type="Rhea" id="RHEA-COMP:15537"/>
            <dbReference type="Rhea" id="RHEA-COMP:15540"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="ChEBI" id="CHEBI:61976"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>N(6)-methyl-L-lysyl(4)-[histone H3] + S-adenosyl-L-methionine = N(6),N(6)-dimethyl-L-lysyl(4)-[histone H3] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:60268"/>
            <dbReference type="Rhea" id="RHEA-COMP:15540"/>
            <dbReference type="Rhea" id="RHEA-COMP:15543"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61929"/>
            <dbReference type="ChEBI" id="CHEBI:61976"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.354" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010008223">
        <property type="protein sequence ID" value="KAI7741290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CI63"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048188">
        <property type="term" value="C:Set1C/COMPASS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140999">
        <property type="term" value="F:histone H3K4 trimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd10518">
        <property type="entry name" value="SET_SETD1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.270.10:FF:000058">
        <property type="entry name" value="Histone-lysine N-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003616">
        <property type="entry name" value="Post-SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044570">
        <property type="entry name" value="Set1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45814">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SETD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45814:SF2">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SETD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50868">
        <property type="entry name" value="POST_SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SET" evidence="11">
        <location>
            <begin position="7"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="Post-SET" evidence="12">
        <location>
            <begin position="133"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7741290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="149" mass="17106" checksum="62671C841DED492E" modified="2024-05-29" version="1">MQRTENDRVCFGKSGIHGWGLFARRNIMEGEMVLEYRGEQVRRSVADLREARYRREGKDCYLFKISEEVVVDATDKGNVARLINHSCMPNCYARIMSVGGDESRIVLIARTNVTTGDELTYDYLFDTDESDDCKVPCLCKAPNCRKFMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-10-08" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CKB5</accession>
    <name>A0AAD5CKB5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Subtilisin-like protease SBT1.9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_019733</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the peptidase S8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007772">
        <property type="protein sequence ID" value="KAI7743212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02120">
        <property type="entry name" value="PA_subtilisin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04852">
        <property type="entry name" value="Peptidases_S8_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000006">
        <property type="entry name" value="Subtilisin-like protease SBT1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.80">
        <property type="entry name" value="Peptidase S8 propeptide/proteinase inhibitor I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034197">
        <property type="entry name" value="Peptidases_S8_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010259">
        <property type="entry name" value="S8pro/Inhibitor_I9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037045">
        <property type="entry name" value="S8pro/Inhibitor_I9_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045051">
        <property type="entry name" value="SBT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041469">
        <property type="entry name" value="Subtilisin-like_FN3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10795">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17766">
        <property type="entry name" value="fn3_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05922">
        <property type="entry name" value="Inhibitor_I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 7" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0720">Serine protease</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Inhibitor I9" evidence="9">
        <location>
            <begin position="5"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="8">
        <location>
            <begin position="104"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="domain" description="Subtilisin-like protease fibronectin type-III" evidence="10">
        <location>
            <begin position="622"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6 7">
        <location>
            <position position="113"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6 7">
        <location>
            <position position="184"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6 7">
        <location>
            <position position="509"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF05922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF17766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7743212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="736" mass="79240" checksum="BF092DDE267513E3" modified="2024-05-29" version="1">MSHETYIVHMDLSAMPXXXXXXXXXXXXXXXXXXVTSSRLVYSYTSAINGFTAILSPYELEQVKNMPGYLYMIKDTTVELDTTHSTQFLGLNSQSGAWPVGSYGEDVIIGVVDTGVWPESESFNDAGMSSVPDRWKGECEDGDEFNSSLCNKKLIGARYFNKGLLSNRPNLTLAMNSARDTDGHGTHTSSTAAGGYVKRASYFGYGTGTAIGVAPNARVAVYKAVWEEGVFVSDILAAIDQAIIDGVDVLSLSLGQDGIPLHQDPIAIATFAAVEKGIFVSASAGNNGPYLGSLHNGTPWVLTVAASTMDREFTGILTLGNGVSVTGFALYPANSSSTNGPIYFVGACENDLDASQIEEKIVVCLDKNDTLGEQYYNVQGTNVLGAIFITNDTDVEFFMQSTYPVLLLDPESAKKVTDYMRKLNNGEAKASMKFHGTRLGTKPAPRVASYSARGPSNSCPVVLKPDITAPGSLILAAWPDSVSLATLKKDVGTGTELLYSKFNLLSGTSMSCPHASGVAALLKAAHPDWTPSAIRSAMMTTSDILDNTLKPIQDIGDYNNPATPLAMGSGHVAPNRALNPGLVYNVNTQDYVNLLCGLNYTNIQIQSITRSSTINCSNPSLDLNYPSFIVYFDGNNTKSVKVIHEFKRTVTYVGIGRSTFTAKLTAIGRLNVSVSPQKLSFQSLNEKKSYKLRIEGSRLMENEVVFGFLSWIDNKGKFVVRSPIVATSLTSTAYMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CLA1</accession>
    <name>A0AAD5CLA1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">HTH myb-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_033354</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007573">
        <property type="protein sequence ID" value="KAI7744153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CLA1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000002">
        <property type="entry name" value="Myb family transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044787">
        <property type="entry name" value="HHO5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058673">
        <property type="entry name" value="HHO5-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006447">
        <property type="entry name" value="Myb_dom_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01557">
        <property type="entry name" value="myb_SHAQKYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31003">
        <property type="entry name" value="MYB FAMILY TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31003:SF19">
        <property type="entry name" value="MYB FAMILY TRANSCRIPTION FACTOR EFM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26575">
        <property type="entry name" value="HHO5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="7">
        <location>
            <begin position="225"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="177"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="369"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="178"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="192"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="209"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="380"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="430"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7744153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="440" mass="47846" checksum="50F921B4A9ECC4DD" modified="2024-05-29" version="1">MGDQQLSTNGHDQTEKLEEFLSCLEEERLKIDAFKRELPLCMQLLTNAMETSRQQLQTYRENKSSPVLEEFIPMNNSNGSGPEKAPNPCIDNKANWMTSAQLWSQAGSTISATNAVSDHVTTQNNNNNNATLIVQNETDINFNAKQRNGGAFHPFSKERNSSCSNPAVVLPELALSSGEKDVDHEENKSCVRENSSIKGGNGEVNDNPTTANTATASGTATSSQTHRKTRRCWSPDLHRRFVNALQMLGGSQVATPKQIRELMKVDGLTNDEVKSHLQKYRLHTRRPSTSPQAAGTATPQLVVLGGIWVPPEYATAAGAQTLYTNPHYGTPQEFYSTAVLPPPHHHHPAAAGNHHHQLNHHNIHMYKPSSQATQSLQASGSGGRGGGTTTAGGNRSESIEDGKSESGGSWKAESGGDTKALMLREECDQESNGSEITLKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CMD5</accession>
    <name>A0AAD5CMD5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_030861</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010007762">
        <property type="protein sequence ID" value="KAI7743254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CMD5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071244">
        <property type="term" value="P:cellular response to carbon dioxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902456">
        <property type="term" value="P:regulation of stomatal opening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009637">
        <property type="term" value="P:response to blue light"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010114">
        <property type="term" value="P:response to red light"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001659">
        <property type="term" value="P:temperature homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd13999">
        <property type="entry name" value="STKc_MAP3K-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000034">
        <property type="entry name" value="Kinase suppressor of Ras 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000310">
        <property type="entry name" value="Serine/threonine-protein kinase HT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF274">
        <property type="entry name" value="DUAL-SPECIFICITY KINASE TKL-PL-4 FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="75"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7743254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="382" mass="42366" checksum="836CEB9A5535E3A0" modified="2024-05-29" version="1">MGSSPKKGSGQLSFSSKELGGNISSKDTFLRADMIDFKSWDVQLDKHLSKVWSCDRDVKEVNAKKQEWEIDLAKLDIRNVIAHGTYGTVYRGVYDGQDVAVKVLDWGEDGLATAAETANLRTSFRQEVAVWHKLDHPNVTKFVGASMGTSDLKIPLNNASNPGHNSLPSRACCVVVEYLPGGTLKKYLIRNSRKKLSFKIVVQLALDLARGLSYLHSKKIVHRDVKTENMLLDSNRTLKIADFGVARVEAQNPRDMTGETGTLGYMAPEVLDGKPYNRKCDVYSFGICLWEVYCCDMPYADLSFAEVSSAVVRQNLRPDIPKCCPSTFANILRKCWDANPDKRPEMDEVVRLLEAIDTSKGGGMIPEGQATGCLCFTTKRGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CNE3</accession>
    <name>A0AAD5CNE3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Poly A polymerase head domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_005806</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007349">
        <property type="protein sequence ID" value="KAI7745148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CNE3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052929">
        <property type="term" value="F:ATP:3'-cytidine-cytidine-tRNA adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052927">
        <property type="term" value="F:CC tRNA cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001680">
        <property type="term" value="P:tRNA 3'-terminal CCA addition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05398">
        <property type="entry name" value="NT_ClassII-CCAase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.460.10:FF:000019">
        <property type="entry name" value="tRNA nucleotidyltransferase cca2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3090.10">
        <property type="entry name" value="cca-adding enzyme, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002646">
        <property type="entry name" value="PolA_pol_head_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13734:SF5">
        <property type="entry name" value="CCA TRNA NUCLEOTIDYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13734">
        <property type="entry name" value="TRNA-NUCLEOTIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01743">
        <property type="entry name" value="PolyA_pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81891">
        <property type="entry name" value="Poly A polymerase C-terminal region-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Poly A polymerase head" evidence="5">
        <location>
            <begin position="46"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7745148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="523" mass="60243" checksum="CBCE9AD53219711C" modified="2024-05-29" version="1">MSKTNGTFSTKCRVVVKENIDLTDKEKQIFDRLLEVVRHFSLETKLRVAGGWVRDKLLGKECYDIDIAIDNLLGREFCEKVNEYLTSTGEETHGIGVIQSNPDQSKHLETARMRLFDVWIDFVNLRSEDYAANSRIPTMQFGSAEQDAYRRDLTINSLFYNINTSSVEDFTGRGLDDLKFGKIVTPLPPKETFLDDPLRVLRAIRFSARFEFEMVEELKVAAADNDVKSAIADKISRERIGHEIDLMVSGNQPAKAMACVSELGLLWVVFTPPKNCEPLISEEHDKVCVRYMDAAWRHMHEAGCSFSDEQRRLYLYASLFIPLRKTVYIDNKKKRVPVVNYIFRNSLKLKASDADDVMRLHNAVKKFLSLIPFVLCKEDEQIGEIDWETDIIDVHLSLKLRILLGLLLREIKDFWRPALMISTLLHNNDSSVEVIEGGLEKRRRVFRDVEQEILKLGLEKVWEVKPLINGKEIIKILELGKGGPVVSEWQRKLLQWQLAYPSGTAEECVDWMTRQTQSKRART</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CPQ4</accession>
    <name>A0AAD5CPQ4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tryptophan synthase</fullName>
            <ecNumber evidence="3">4.2.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_000014</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine = D-glyceraldehyde 3-phosphate + L-tryptophan + H2O</text>
            <dbReference type="Rhea" id="RHEA:10532"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:58866"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.2.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 5/5.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.20" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010007378">
        <property type="protein sequence ID" value="KAI7745035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CPQ4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004834">
        <property type="term" value="F:tryptophan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000004">
        <property type="entry name" value="Tryptophan synthase beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023026">
        <property type="entry name" value="Trp_synth_beta/beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48077:SF4">
        <property type="entry name" value="TRYPTOPHAN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48077">
        <property type="entry name" value="TRYPTOPHAN SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001413">
        <property type="entry name" value="Trp_syn_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="10">
        <location>
            <begin position="102"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7745035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="388" mass="42015" checksum="562BD62DCBC7F7C4" modified="2024-05-29" version="1" fragment="single">TISTVSGEKNPIVGKFGSFGGKFVGEVLISPLAELESEFNSVLLDHDFKMEVETALRDYVGRETPLYHAQKLTDHYKNINGEGPEIYIKREDLSHGGSYKMNNVVAQAILAKRMGRKSIITATNMLRKPSNVQLMELLGAQKVTKSIKQRKKVKVKPVEGSFQAAASESTREWISNLQTGYYLAGTAVGPHPIPTMVREFNSVIGKETRRQAMEKWGGSPDVLVACVGTGCNALGLFHEFVGDKNVRMIGVEGGGVGTNTGGDQLHSASLVRGEVGVYHGAMCYLLQDEQGQITKTQSIASGLEFPGVSPELSFLKDAGRIECYTVTDQEALDAYKRVCRLEGIVPALEASHAFAYLEKLCPKLPHGAKVVVNCSGTGYNDAATVLNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CPV0</accession>
    <name>A0AAD5CPV0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_008236</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007208">
        <property type="protein sequence ID" value="KAI7745537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CPV0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7745537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="55" mass="6065" checksum="A9D248E6CA469FB5" modified="2024-05-29" version="1">MKDGGGDCLQWPQGCRLERMSAALSETPRLDMLIMELIPPASVDSVADDLQLYQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CQ80</accession>
    <name>A0AAD5CQ80_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glycoside hydrolase family 3 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_000418</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007014">
        <property type="protein sequence ID" value="KAI7746253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CQ80"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008422">
        <property type="term" value="F:beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009251">
        <property type="term" value="P:glucan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.300">
        <property type="entry name" value="Glycoside hydrolase, family 3, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051915">
        <property type="entry name" value="Cellulose_Degrad_GH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001764">
        <property type="entry name" value="Glyco_hydro_3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036962">
        <property type="entry name" value="Glyco_hydro_3_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30620:SF100">
        <property type="entry name" value="GLUCAN 1,3-BETA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30620">
        <property type="entry name" value="PERIPLASMIC BETA-GLUCOSIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00933">
        <property type="entry name" value="Glyco_hydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00133">
        <property type="entry name" value="GLHYDRLASE3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycoside hydrolase family 3 N-terminal" evidence="2">
        <location>
            <begin position="2"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7746253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="110" mass="11835" checksum="F5DF8C56EEF5B130" modified="2024-05-29" version="1" fragment="single">NRDPELVKKIGAATALEVRATGINYAFAPCVAVCRDPRWGRCFESFSEDPNIVRAMTEIIPGLQGEIPVNGRKGVPFVDGQTKVASSAKHFVGDGGTTRGINENDTVIDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CQI1</accession>
    <name>A0AAD5CQI1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ATP-dependent Clp protease proteolytic subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_013131</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Component of the chloroplastic Clp protease core complex which consist of at least 16 proteins: CLPP4 (3 copies), CLPP5 (3 copies), CLPR4 (2 copies), ClpP1 (1 copy), CLPP6 (1 copy), CLPR2 (1 copy), CLPT1 (1 copy), CLPT2 (1 copy) and 3 copies of CLPP3 and/or CLPR1 and/or CLPR3. The core complex is organized in two heptameric rings, one containing CLPP3,4,5,6 in a 1:2:3:1 ratio and the other CLPP1 and CLPR1,2,3,4 in a 3:1:1:1:1 ratio.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the peptidase S14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007466">
        <property type="protein sequence ID" value="KAI7744666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CQI1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009368">
        <property type="term" value="C:endopeptidase Clp complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009532">
        <property type="term" value="C:plastid stroma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051117">
        <property type="term" value="F:ATPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07017">
        <property type="entry name" value="S14_ClpP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000020">
        <property type="entry name" value="ATP-dependent Clp protease proteolytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001907">
        <property type="entry name" value="ClpP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023562">
        <property type="entry name" value="ClpP/TepA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10381">
        <property type="entry name" value="ATP-DEPENDENT CLP PROTEASE PROTEOLYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10381:SF55">
        <property type="entry name" value="ATP-DEPENDENT CLP PROTEASE PROTEOLYTIC SUBUNIT-RELATED PROTEIN 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00574">
        <property type="entry name" value="CLP_protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00127">
        <property type="entry name" value="CLPPROTEASEP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00062827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7744666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="384" mass="42188" checksum="36282419071F72EE" modified="2024-05-29" version="1">MSTSTLLSPLSPPSVQNKSCFVVGNAIRKDATANLNATGTRRCFRSTPVTAMASDRFLDYIPKQFREPNLKDGLMENFKNVPQYLYGLSPSQMDMFMTEDNPVRRQAESVTEETISSSHNYLNHGGMWSLSGMSDMGPAKYSMSVSMYRGGGRAAGRPRSAPPDLPSLLLDARICYLGMPIVPAVTELLVAQFMWLDYDNPSKPIYLYINSSGTQNDKGETVGSETEAYAIADIMAYCKSEVYTVNCGMAFGQAAMLLSLGTKGYRALQPNSSTKLYLPKVTRSSGAAIDMWIKAKELDTNTDYMLELLEKGIGKPKDEINKDIMRPKYFQAQEAIDYGIADKIISSRDNAFEKRNYDEILAQSKAARRAAGGGGAQAAPSGFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CTG5</accession>
    <name>A0AAD5CTG5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_005723</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006942">
        <property type="protein sequence ID" value="KAI7746569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CTG5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7746569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="123" mass="13758" checksum="BD32786778A6E9CD" modified="2024-05-29" version="1" fragment="single">MKITSESTPATPTTRRPHPAHTPSSPPLKTLTRPETTNIFSPSTTSPSQPFIHLLQHYRKSLSRSGSFVEAIDYSIIQLHFDDQLHKLIVFGPHLEVGSSTVMLIARKLAHNIQIPSLARDQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CUB8</accession>
    <name>A0AAD5CUB8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptide chain release factor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_007601</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the prokaryotic/mitochondrial release factor family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006526">
        <property type="protein sequence ID" value="KAI7748443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CUB8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016149">
        <property type="term" value="F:translation release factor activity, codon specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000004">
        <property type="entry name" value="Peptide chain release factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1660:FF:000002">
        <property type="entry name" value="Peptide chain release factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1660">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00093">
        <property type="entry name" value="Rel_fac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005139">
        <property type="entry name" value="PCRF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000352">
        <property type="entry name" value="Pep_chain_release_fac_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045853">
        <property type="entry name" value="Pep_chain_release_fac_I_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050057">
        <property type="entry name" value="Prokaryotic/Mito_RF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004373">
        <property type="entry name" value="RF-1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00019">
        <property type="entry name" value="prfA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001859">
        <property type="entry name" value="PRK00591.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43804">
        <property type="entry name" value="LD18447P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43804:SF7">
        <property type="entry name" value="LD18447P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03462">
        <property type="entry name" value="PCRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00472">
        <property type="entry name" value="RF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00937">
        <property type="entry name" value="PCRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75620">
        <property type="entry name" value="Release factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0488">Methylation</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptide chain release factor" evidence="5">
        <location>
            <begin position="116"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="97"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7748443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="415" mass="46882" checksum="CC5AF1B5C99095A1" modified="2024-05-29" version="1">MRVGGFRVLCRHLVRSFSLNGNHHHLRTKIHPFFIPPYSTVAEVQPQSSAELIKIMEHRLSAIEHRNAYLQGIINQPETSPTEYSRANKEIQSLSDKMDLICKLRKVEKEIESLKSLVGDCEEDKDMQAMAYEELEQALKEEQNLHNLLLKSLLPKDDADVRDCILEVRAGTGGEEASLFAMDIFKMYERYSQKKGWRFEVVEIADSNMKGFKEASAAISGADVYGKLKFESGVHRVQRVPVTEKSGRVHTSAVSVAILPQADEVDVQLRNEDLRIDTYRSGGSGGQHANTTNSAVRITHIPSGLTVSIQDERSQHMNKAKALKVLCARLYEMERCRAHNSRSKLRSEQIGSGDRSERIRTYNFPQGRVTDHRIGFTHHSISDVMQGESLDYFIDALLLQQEMDAIADFTATSAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CUP2</accession>
    <name>A0AAD5CUP2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Helicase ATP-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_012436</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006496">
        <property type="protein sequence ID" value="KAI7748636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CUP2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.220.10">
        <property type="entry name" value="Leucine Aminopeptidase, subunit E, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031053">
        <property type="entry name" value="ALC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043472">
        <property type="entry name" value="Macro_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47157">
        <property type="entry name" value="CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47157:SF1">
        <property type="entry name" value="CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52949">
        <property type="entry name" value="Macro domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="8">
        <location>
            <begin position="52"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="564"/>
            <end position="592"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7748636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="764" mass="85731" checksum="45DDDE7109C26E0F" modified="2024-05-29" version="1">MDYEQRLIAAAKYVFDGDSRAAADDDGEVNCSDYGVTAVLKPHQIEGLSWLIRRYLIGANVILGDEMGLGKTLQAISFLSYLKFCRGLHGPFLILCPLSVTDGWISEVTKFSPKLKVLRYVGDKQYRRALRREMYENVEKQSSSTNVQALPFDVLLTTYDIALIDQDFLSQIPWHYAVIDEAQRLKNPSSVLYNALREQYIMPRRLLMTGTPIQNNLTELWALMHFCMPSIFGTLEQFSSTFKEGKGALVKEKFKSLKYVLGAFMLRRTKSKLIEIGTLSLPPLTEITLMAPLVTLQKKVYMSILRKELPKLLALSSGTANQQSLQNIVIQLRKACSHPYLFAGIEPEPYEEGEHLIQASGKLIVLDQLLQKLRNSGHRVLLFAQMTHTLDILQDYMELRKYPYERLDGSVRAEERFAAIRITERTVEEVIMHRAERKLQLSHDVIGEEAIDKEGKDMVGAEAGDLKSVVLGLRMLDPTTDSNESLDQLDMSKVNSIVEKVIAFRHGGKSEVEDRTFEINSNDLLGGRSNDPILDEASYNSWVQKLKETSDNSGDLALEEANKRSKLEEKHLKAESVRKKAEEKKLAKWEAQGYTSLAVRDVVPPQDNILSDSGLVNLVYGDCTQPSKICPSEPCIIFSCVDNSGEWGHGGMFDALARLSSQIPKAYEHASEFGDLHLGDLHLIEITAGEDEENKVANVGQWVALAVVQTYNPRRKVPRSSISIPELEQCLSKASFSAAQKSGIISDDPLKSGDEAMYWVLEVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CX58</accession>
    <name>A0AAD5CX58_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_021881</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006324">
        <property type="protein sequence ID" value="KAI7749579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CX58"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026894">
        <property type="entry name" value="DnaJ_X"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052423">
        <property type="entry name" value="EMIR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44094:SF17">
        <property type="entry name" value="CHAPERONE PROTEIN DNAJ 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44094">
        <property type="entry name" value="DNAJ HEAT SHOCK N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14308">
        <property type="entry name" value="DnaJ-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="1">
        <location>
            <begin position="12"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7749579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="296" mass="33413" checksum="DCBBC8FA16BB99CE" modified="2024-05-29" version="1" fragment="single">IIINNIITEETEYYDILGVKPTATESEIKKAYYIKVLGEAYQVLSNPDQRQAYDTHGKSGIATEAIIDPAAIFAMLFGSELFEDYIGQLAMASMASMDIFTEGEQVDVKKVQEKMRVQIHKLDNFISLLKCFKTLIKHFNIGAIALMQLQEDLKRQLSAEGKYTEQQLEEYMESHKKIMIDSLWKLNVADIEATVSRVCQTVLHDHDVKKEELRARAKGLRILGRIFQKAKPANGNESEPMLQSGMHMNGIDTDHATRAKTTMVPQSPYVEAPQILGVENYDYPMPTVPPGAQRSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CXA4</accession>
    <name>A0AAD5CXA4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">40S ribosomal protein S23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_022010</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006577">
        <property type="protein sequence ID" value="KAI7748221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CXA4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03367">
        <property type="entry name" value="Ribosomal_S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000007">
        <property type="entry name" value="40S ribosomal protein S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006032">
        <property type="entry name" value="Ribosomal_uS12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005680">
        <property type="entry name" value="Ribosomal_uS12_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00982">
        <property type="entry name" value="uS12_E_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11652">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S12 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00164">
        <property type="entry name" value="Ribosom_S12_S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00055">
        <property type="entry name" value="RIBOSOMAL_S12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="187"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="245"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="313"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="344"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="406"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7748221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="486" mass="52853" checksum="1BABC6B1CE41045F" modified="2024-05-29" version="1">MGAGRKLKSHRRRQRWADKSYKKSHLGNEWKKPFAGSSHAKGIVLEKIGIEAKQPNSAIRKCARVQLIKNGKKIAAFVPNDGCLNYIEENDEVLIAGFGRKGHAVGDIPGVRFKVVKVSDQIKVNDKKMRRSFTWDRAFYTSDGFLAAEELLSMIEGGGDDDKHELKKLDELESLEAKLFQEIEASIQKATGPSGKRKSQEKKITGAGGPHRVPPIKTLPTRLSLSSAGSGGSRGNNIKTPVRGSMSSKNGKANAYSPSKTPPVITTKNKVASVNTRVSRTSPASPVSTWSPGSSPLTFAVNQRLKRSGSVPHKSSMNNEKPSATNERGSDDRKASKSAVSLMPKTQSSPTIQRPSESSSCAVYQRSRSQGSKGSPVHHISKHTLKNYPIGQSSDLNRKQPAKTAATANTGLKPNTPKTKKQSSPKVKASSEKIFVISPEILDLKGKINALKMKIDMHKDRCNKMARAESDSGYLKTSFAINECSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CYQ0</accession>
    <name>A0AAD5CYQ0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">NB-ARC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_002695</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006363">
        <property type="protein sequence ID" value="KAI7749419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CYQ0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057135">
        <property type="entry name" value="At4g27190-like_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050905">
        <property type="entry name" value="Plant_NBS-LRR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463:SF222">
        <property type="entry name" value="NB-ARC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23247">
        <property type="entry name" value="LRR_RPS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0611">Plant defense</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NB-ARC" evidence="5">
        <location>
            <begin position="50"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein At4g27190-like leucine-rich repeats" evidence="6">
        <location>
            <begin position="450"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="676"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7749419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="676" mass="77685" checksum="82CFD396542E5BFF" modified="2024-05-29" version="1" fragment="single">YNQELCLTKVSSEKASTSTQVHEDEDDGTQSIIKSSRDLIFNDALISLESNDNKARMMALSGMGGVGKTTMMEQLVKAVDDRKTFNWIVKVAVGNNRNPVDIQETEDVCTQMDVETSLIYRIGVLKAEEAKKLFWDTARLGSNSDIEQLDLQHIGEEILKICGGLPLAIKTIASTLRDKEKFEWEDALSRLQNKNVDDYGIKEIFVISFENLKEDDLKAIFLLSGLFPEDSNIEKEDLLRMKKLEVVSYLNMHRPFLPQCLTNLRALCLRSCSLMHNKVSILGDLINLEVLDLAGCGINKLPSKIGQLRRLKLLDLSGCVNLHIDNGVFENLKQLEELYMGVCEASSVRLSDTNCDELKILSRQLFALELEFFNNKAQPVNVSFENLERFKISMGRSLEKRWRSDRSDGYYSFKNTLILKTSCSELRECKINELFRKTEILQLEVLIPKLEKLTITSMESLKEIWECEITTSEGEVNNIFMLREIEMSDCRSVVNLFPNNPMRLLTHLEELSVIKCGSIEVLFNIELGQIQQLSRCSLRNIKVWICESLREVWRIKGAEHNYFISDFQDVERIVIQECERFRNIVRPTTVNFDMKALKDIRIGSCDEYGERNNELCKNSQEQEINNISKERISKVDDSLSTAAFTSNHMASLFHQLRYIWFERVKGVKVVFEMESP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CZ37</accession>
    <name>A0AAD5CZ37_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">E2 ubiquitin-conjugating enzyme</fullName>
            <ecNumber evidence="1">2.3.2.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_002833</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="1"/>
    <dbReference type="EMBL" id="JAMZMK010006393">
        <property type="protein sequence ID" value="KAI7749226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CZ37"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd23797">
        <property type="entry name" value="UBCc_UBE2H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000024">
        <property type="entry name" value="Ubiquitin-conjugating enzyme 5, E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 8" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="10">
        <location>
            <begin position="1"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="152"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="158"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="7">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00056190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7749226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="186" mass="20909" checksum="B4BF3729E97090AD" modified="2024-05-29" version="1">MSSPSKRREMDVMKLMMSDYVVEPINDGINELNVEFHGPKESLYEGGLWKIRVELPDAYPYKSPSIGFLNRIFHPNVDELSGSVCLDVINQSWSPMFDLLNVFEVFLPQLLLYPNPSDPLNGDAASLMIKDKEQYEKKVKEYCELYAKKGNISGTISDDSDEDESNDDVSDGRSESSEDVAGNADP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CZI0</accession>
    <name>A0AAD5CZI0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FAD-binding PCMH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_026978</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; L-ascorbate biosynthesis.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006174">
        <property type="protein sequence ID" value="KAI7750262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CZI0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003885">
        <property type="term" value="F:D-arabinono-1,4-lactone oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016633">
        <property type="term" value="F:galactonolactone dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.10">
        <property type="entry name" value="Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007173">
        <property type="entry name" value="ALO_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016167">
        <property type="entry name" value="FAD-bd_PCMH_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010031">
        <property type="entry name" value="FAD_lactone_oxidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010029">
        <property type="entry name" value="GL_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01676">
        <property type="entry name" value="GLDHase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43762:SF1">
        <property type="entry name" value="D-ARABINONO-1,4-LACTONE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43762">
        <property type="entry name" value="L-GULONOLACTONE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04030">
        <property type="entry name" value="ALO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000136">
        <property type="entry name" value="LGO_GLO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="4">
        <location>
            <begin position="119"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7750262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="613" mass="69357" checksum="C475300E7620C802" modified="2024-05-29" version="1">MFRSLKLKRPLHSFLNNNNINQTPHFLYSLNPLISSPYPQTNLIRPFASSPPSSDPPLSSTSSSELRKYLGYSALLLTCAAATYYSFPFPDNAKHKKAQLFRYAPLPVDLHSIVNWSGTHEVQTRVFHQPESLDQLEQVVNDCNEKKQKIRPVGSGLSPNGIGLARGGMVNLALMDKVLEVDKVKKTVRVQAGMRVQQVVDEIKGFGLTLQNFASIREQQIGGIVQVGAHGTGAKLPPIDEQVISMKLVTPGKGTIEISKDKDPELFYLARCGLGAFGVVAEVTLQCVERQELVEHTYVSTLAEIKKKHKKLLNDNKHVKYLYIPYTDTVVVVTCNPVSKWWGSNKFKPKYTSDEALQPIRDLYKASKEKYRGQSVTTSSTVEDEPKISDLSFTELRDELIALDPLNKDHIKKINEAEAEFWKRSEGYRVGWSDEILGFDCGGQQWVSETCFPAGTLSKPNMKDLQYIEDVMRLIEKEEIPAPAPIEQRWTASSKSLMSPASSSSNDDIFSWVGIIMYLPTTDARQRKKITEEFFHYRHLTQARLWDQYSAYEHWAKIEVPKDKDELAALQARLRKRFPVDAFNKARAELDPNRILSNAMLEKMFPLDENAST</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D0C0</accession>
    <name>A0AAD5D0C0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">GOLD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_027796</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the EMP24/GP25L family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005955">
        <property type="protein sequence ID" value="KAI7751184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D0C0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015720">
        <property type="entry name" value="Emp24-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22811">
        <property type="entry name" value="TRANSMEMBRANE EMP24 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01105">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01190">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50866">
        <property type="entry name" value="GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042099410" description="GOLD domain-containing protein" evidence="8">
        <location>
            <begin position="24"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="GOLD" evidence="9">
        <location>
            <begin position="33"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7751184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="165" mass="18511" checksum="946407A817F974C5" modified="2024-05-29" version="1" fragment="single">MKLQTSFLCIIVIIALVSTTTQSIRFEIESGHTKCIAEDIKSNSMTVGHYSIVNPNEGHPLPESHRITLRVTSSYGNSYHSSENVQSGQFAFQAVEAGDYMACFFAIDHQPPLKFQVEFDWRSGVAAKDWSNVAKKGSVDAMEYELKKLADTITSIHDEMLYLRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D304</accession>
    <name>A0AAD5D304_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_026714</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005567">
        <property type="protein sequence ID" value="KAI7752973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D304"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010765">
        <property type="entry name" value="DUF1350"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34127:SF3">
        <property type="entry name" value="INITIATION FACTOR 4F SUBUNIT (DUF1350)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34127">
        <property type="entry name" value="OS04G0405600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07082">
        <property type="entry name" value="DUF1350"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7752973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="385" mass="42218" checksum="EF154749EA91DF53" modified="2024-05-29" version="1">MVIASTRIGTCGSMELAPASFSTRHVGRDCVYLKSISSPLLQKQPRWFGFSRDRNSTFSHSACSLRMVSNDYVGRSTYRIKKDNDNKLYRRLDSCLVIPPPKGKKPKAIIKFLGGAFIGAVPEVTYSYLLGLLANEGYLIISVPYNVTFDHSQAAREVYERFHLCLNSILTSGLPDDGLLASELVDLPLYSVGHSNGALLQVLSGSYFSDKLPKANAIISYNNRPATEAVPYFEQLGPLVSQLMPVVEASPVSSMAKGAADAWKALLDTAQAMTPDYDPEARVSLDKFVDQLPSVFNQVKFNVDAIDETDLLEETLRPRVEAIGGTLEKVSLSGNHITPCIQEPKLQVGEVYTPADAIAQGLKNLSLNETKIMARTIVDWFSSIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D3P9</accession>
    <name>A0AAD5D3P9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">histidine kinase</fullName>
            <ecNumber evidence="3">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_015863</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010005665">
        <property type="protein sequence ID" value="KAI7752512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D3P9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009927">
        <property type="term" value="F:histidine phosphotransfer kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16922">
        <property type="entry name" value="HATPase_EvgS-ArcB-TorS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000061">
        <property type="entry name" value="Histidine kinase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000201">
        <property type="entry name" value="Histidine kinase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000069">
        <property type="entry name" value="Probable histidine kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.130:FF:000030">
        <property type="entry name" value="Putative histidine kinase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43047:SF68">
        <property type="entry name" value="HISTIDINE KINASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43047">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Histidine kinase" evidence="11">
        <location>
            <begin position="375"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="12">
        <location>
            <begin position="731"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="80"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="185"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="9">
        <location>
            <position position="780"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7752512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="872" mass="99360" checksum="E37A45448AB12FE0" modified="2024-05-29" version="1">MEDDNAEDMEVEILSSMWPEDIDAAGKKFNVEKPGVDQDLLEDLTFNEEPTIVDFQRLLELTNYSEKGSSQLSYLVKNWEWKQENAVRLLREELENLRKQQHGFELKKLEILEEHRFENDDYGCDKRPVSILDEVYEIFQDVPKKRTDVIVQDETLEIDDECDANTVRYWKQRALHFEKLLSISVQREQMLLEKLEESIEKLEKQSSPVEELSQILKRADNFLHFVLQNAPVVIGHQDKELRYRFIYNHFPSLGEEDIIGKTDVEIFKGGGVKESQDFKKEVLEKGIPGKREITFETELFGAKTFLIYVEPVFSKAGETIGVNYMGMEITDQVKKREKMAKLREEIAVQKARETELNKTIHITEETMRAKQILATMSHEIRSPLSGIISMAEILSTTKVDKDQKQLLSVMLSSGDLVLQLINDILDFSKVESGVMKLEATKFRPREVIKHVLQTAAASLKKMLILEGHVADDVPVEVIGDVLRIRQILTNLISNAVKFTHEGKIKIKLYLITDPCLEDQIKHEENEPQSHGNMVWIRCDVQDTGIGIPETALPLLFKKYMQAGTDTARKYGGTGLGLAICKQLVELMGGRLTVTSKVNHGSTFTFVLPHKVPQACDSSDETDDYQDGDDNGSMDEDDVNCGFFQFKTQTLGSLFSCNGYGPTHELTRNEVGSDKLDRASVLENNNTNHMTVDQDEDRPFHDPCIKKNRPSNLNMMRVDSSTTTSKSSARHKILLVEDTVVNVMVAKRMMKQLNQDMDVVNNGVEALRAVQRCDYSLILMDVCMPVMDGLQATRLIRSYEKNSNWDEARLAGVDITDQMSREPRKRIPIIAMTANAMSESAEECFTNGMDSFITKPVTLQKLKQCLEQYLPSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D635</accession>
    <name>A0AAD5D635_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_026833</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the plant LTP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005613">
        <property type="protein sequence ID" value="KAI7752751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D635"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00010">
        <property type="entry name" value="AAI_LTSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.110.10">
        <property type="entry name" value="Plant lipid-transfer and hydrophobic proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036312">
        <property type="entry name" value="Bifun_inhib/LTP/seed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016140">
        <property type="entry name" value="Bifunc_inhib/LTP/seed_store"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043325">
        <property type="entry name" value="LTSS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33044">
        <property type="entry name" value="BIFUNCTIONAL INHIBITOR/LIPID-TRANSFER PROTEIN/SEED STORAGE 2S ALBUMIN SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14368">
        <property type="entry name" value="LTP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00499">
        <property type="entry name" value="AAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47699">
        <property type="entry name" value="Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042078741" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein" evidence="9">
        <location>
            <begin position="25"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical" evidence="10">
        <location>
            <begin position="33"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7752751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="188" mass="20409" checksum="E4C7FE17D3E139D4" modified="2024-05-29" version="1">MYNKLFVLVTVIVVWMVTYRGVEAAEDSLAQECSKKLTAVMTCVDFATGKEATPQKKCCDSVKEMKDSNPACLCFVIQQIHNGTNPMLKSINIQESRLLQLSSACNIANASISDCPRLLKLPPNSPDASIFTNNATIVPPTSGRTPSSTTSALGSSRALKYKAPGILRSTWIPLIFLLVVSSKSVFYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D6G8</accession>
    <name>A0AAD5D6G8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AMMECR1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_032707</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010001545">
        <property type="protein sequence ID" value="KAI7755228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D6G8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.700.20:FF:000001">
        <property type="entry name" value="AMME syndrome candidate gene 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.700.20">
        <property type="entry name" value="Hypothetical protein ph0010, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023473">
        <property type="entry name" value="AMMECR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036071">
        <property type="entry name" value="AMMECR1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002733">
        <property type="entry name" value="AMMECR1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027485">
        <property type="entry name" value="AMMECR1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13016:SF4">
        <property type="entry name" value="AMMECR1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13016">
        <property type="entry name" value="AMMECR1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01871">
        <property type="entry name" value="AMMECR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143447">
        <property type="entry name" value="AMMECR1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51112">
        <property type="entry name" value="AMMECR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMMECR1" evidence="1">
        <location>
            <begin position="1"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7755228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="146" mass="16929" checksum="49CDD983D55F5E1A" modified="2024-05-29" version="1" fragment="single">LFCHLIFFTLDRLYSSTIYTYDFSPLFVTWKKVVNGYEPRLRGCIGTLSARDIVNGFKDYALTSALRDHRFPPIQAKELPFLQCTVSILTNYEDAANYLDWEVGKHGIIIEFTDPDYNTKRSATYLPDVAAQEGWFCCLLKWIFCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D9W2</accession>
    <name>A0AAD5D9W2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_008043</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000167">
        <property type="protein sequence ID" value="KAI7757178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D9W2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02947">
        <property type="entry name" value="TRX_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000245">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050620">
        <property type="entry name" value="Thioredoxin_H-type-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10438:SF465">
        <property type="entry name" value="MONODEHYDROASCORBATE REDUCTASE (NADH)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10438">
        <property type="entry name" value="THIOREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0249">Electron transport</keyword>
    <keyword evidence="3" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Thioredoxin" evidence="4">
        <location>
            <begin position="19"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7757178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="139" mass="15362" checksum="CDC1BB5B5E8D763D" modified="2024-05-29" version="1">MGLCFSSTKDDEEPACHPHFTGGKVTVITTKDAWNQKLSEAKTQSKIVIANFSASWCGPCKSISTLYIELSEKHPSLMFLTIDVDELPELSTQWDVKATPTFFFLKDGQQIDKVVGANKPELQEKLTAIVDSNVPGDQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DAJ8</accession>
    <name>A0AAD5DAJ8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">peptidyl-tRNA hydrolase</fullName>
            <ecNumber evidence="1">3.1.1.29</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_001471</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>an N-acyl-L-alpha-aminoacyl-tRNA + H2O = an N-acyl-L-amino acid + a tRNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54448"/>
            <dbReference type="Rhea" id="RHEA-COMP:10123"/>
            <dbReference type="Rhea" id="RHEA-COMP:13883"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:59874"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:138191"/>
            <dbReference type="EC" id="3.1.1.29"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PTH2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.29" evidence="1"/>
    <dbReference type="EMBL" id="JAMZMK010000112">
        <property type="protein sequence ID" value="KAI7757486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DAJ8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004045">
        <property type="term" value="F:peptidyl-tRNA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd02430">
        <property type="entry name" value="PTH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1490.10:FF:000002">
        <property type="entry name" value="Peptidyl-tRNA hydrolase 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1490.10">
        <property type="entry name" value="Bit1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023476">
        <property type="entry name" value="Pep_tRNA_hydro_II_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002833">
        <property type="entry name" value="PTH2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00283">
        <property type="entry name" value="arch_pth2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003314">
        <property type="entry name" value="PRK04322.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12649">
        <property type="entry name" value="PEPTIDYL-TRNA HYDROLASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12649:SF11">
        <property type="entry name" value="PEPTIDYL-TRNA HYDROLASE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01981">
        <property type="entry name" value="PTH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102462">
        <property type="entry name" value="Peptidyl-tRNA hydrolase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7757486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="189" mass="20497" checksum="9B411639BD3DA3FF" modified="2024-05-29" version="1">MGSFGNKSQPSKRKVDKGWLGQSFQPQNFIPGLVIGFIFGILLDLTKPITRNSSIIGRRKNNGVLSNSSNPDEDFKMVLVVRQDLKMGQGKVASQCAHAATGLYAKLMQSNPSLLRRWEHCGQAKIVVTCKNQQEMNKLMEAAEGFGLPTYVVADAGRTQVSAGSKTVLAIGPGYKSVVDSVTGKQRLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DAV2</accession>
    <name>A0AAD5DAV2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phospholipase/carboxylesterase/thioesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_003179</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000087">
        <property type="protein sequence ID" value="KAI7757673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DAV2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003140">
        <property type="entry name" value="PLipase/COase/thioEstase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46234">
        <property type="entry name" value="ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02230">
        <property type="entry name" value="Abhydrolase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phospholipase/carboxylesterase/thioesterase" evidence="1">
        <location>
            <begin position="26"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7757673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="199" mass="21418" checksum="3EAE88ACB869FF34" modified="2024-05-29" version="1">MSYNRSNIGSGSRNVGRHTFEFGRTYVVRPRGTHQATIVWLHGIGEMGSSWTQFLESLPIPNIKWICPTAPTRPITLFGGNPSTAWFDIEDLSDNATDDVEGLDASAAHIVNMLSNERDDIKLGVAGFSMGAAMALYSATCRAIGQFGNGNRYPINLNVAVALSGWLPCSRIVRSRVPASQEAGRRAAALAVLVCHGQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DBG8</accession>
    <name>A0AAD5DBG8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">HTH myb-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_017079</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000105">
        <property type="protein sequence ID" value="KAI7757533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DBG8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000002">
        <property type="entry name" value="Myb family transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044787">
        <property type="entry name" value="HHO5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058673">
        <property type="entry name" value="HHO5-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006447">
        <property type="entry name" value="Myb_dom_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01557">
        <property type="entry name" value="myb_SHAQKYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31003:SF3">
        <property type="entry name" value="HOMEODOMAIN-LIKE SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31003">
        <property type="entry name" value="MYB FAMILY TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26575">
        <property type="entry name" value="HHO5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="7">
        <location>
            <begin position="200"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="283"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="287"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7757533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="317" mass="36090" checksum="8F9E22F35C8E9AF4" modified="2024-05-29" version="1">MATLNPFPELTLDFHPVFIPKSVSQFLGELSRIGSVTEKVLQVNDFVNRLESETHKLEAFGRELPLCMLLAKDAIVALKEETMVLKKSLNVQPVLEEFMPLKNFSDHDDDDDKKVETGTNQKDDVDKKSWLSSTHLWNTNENFNTKVNSIAQMVNKKNEEEDLRREIVNENNRLISCFVPNVQPNMQIVSTVVAPLQQHTFRKQRRCWSNELHRRFVNALQQLGGSQVATPKQIRELMKVDGLTNDEVKSHLQKYRLHTRRIPSSKANQSCVDVGGPWMSKCSNSKSGSPNGPLLNGGNNIDEEDEKSENYCWSGNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DCV8</accession>
    <name>A0AAD5DCV8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BYPASS-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_023020</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010001055">
        <property type="protein sequence ID" value="KAI7755525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DCV8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31509">
        <property type="entry name" value="BPS1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7755525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="327" mass="37639" checksum="D2BB9D1EABCCE6E2" modified="2024-05-29" version="1">MFHQHMMKSSSSTTTSSSSSLNNFYSSLTHSLDDLYGAFDSQNIMSFHFLQLVLASLQSFYSEITLLVHKLHLPIGEKWLDEYMDESARLWEVCHVLKSSISNMEIYCSMGANITSILENRDLSPQISREVLTAINRCQRESVRLEEENKSLGETRIKPLTMKFEETNLIHSKFNGFNGFRGALYAFKNISSLLLKIMVNGLVYCSNETSLSNLPHINTTSYSENRAIFRTDFMVSAARLNERVNEREDEQDGVLLKEFRETMNSMDELKRELERIRGFETKCDISGRVENLKNCFGGFQCGIENTIVQLDDFFDEIVESRKKLLEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DDQ5</accession>
    <name>A0AAD5DDQ5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_025046</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000244">
        <property type="protein sequence ID" value="KAI7756861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DDQ5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01837">
        <property type="entry name" value="SGNH_plant_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050592">
        <property type="entry name" value="GDSL_lipolytic_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035669">
        <property type="entry name" value="SGNH_plant_lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45642">
        <property type="entry name" value="GDSL ESTERASE/LIPASE EXL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45642:SF90">
        <property type="entry name" value="GDSL LIPASE_ESTERASE, SGNH HYDROLASE SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041925565" evidence="2">
        <location>
            <begin position="26"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7756861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="356" mass="39777" checksum="FBBAC1050A37D6DC" modified="2024-05-29" version="1">MSADLLILLILILIVNILSVQYCNAQQNTISAVFIFGDSTVDSGNNDYIPTISRGNFPPYGKDFDNQQPTGRFSNGRLVPDFVAEFVGLKKNLPPYLDPSLTIDDLMTGVCFASAGAGYDPLTSQISMALTQSQQLDLFKEYKVKMEAAIGKERTDDLVRRAGYLTSSGTNDFTFNYYGPLPVRRTHYPTISQYKQFQWQQLEQFLQDLLNEGAQKIGVVGLPPIGCLPAIITLNSKSPIFGRECNETYNSLARDVNQMLQDNLKSLERPGTKIVYADIYTPIINMVEQKAQYGFEESHKGCCGTGLIEADFGCNPKSPLCDDVLKYVFWDAFHPTEKGYRIIFDSLKSLIQENLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DE07</accession>
    <name>A0AAD5DE07_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_033352</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000030">
        <property type="protein sequence ID" value="KAI7758087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DE07"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7758087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="69" mass="7585" checksum="0325B81DC25B6F62" modified="2024-05-29" version="1">MYGGCRLEIGDDFASRMVMGKVKKVKTFVEAHSCTRSNKGSNKLATQGWIAKIVVRDKLKSNGVVSTHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DED3</accession>
    <name>A0AAD5DED3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Sulfotransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_019292</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000053">
        <property type="protein sequence ID" value="KAI7757845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DED3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017095">
        <property type="term" value="F:heparan sulfate 6-sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010635">
        <property type="entry name" value="Heparan_SO4-6-sulfoTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12812">
        <property type="entry name" value="HEPARAN SULFATE 6-O-SULFOTRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12812:SF12">
        <property type="entry name" value="TYROSYLPROTEIN SULFOTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042094972" description="Sulfotransferase" evidence="8">
        <location>
            <begin position="26"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="317"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="317"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7757845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="452" mass="51610" checksum="4FBE9BE2D7C21134" modified="2024-05-29" version="1">MENGYALRFAFLLLLLVLVFTSSGANDFEHCKSSIKEWASSSLRSDTDTKNGHILKDLLFFLHVPRTGGRTYFHCFLKKLYSSDLECPRSYDKLRFNPRKPGCRLLATHDDYSMMSKLPREQTSVVTILRDPVERVFSTYEFSVEVAARFLVHPNLTSVIKMSNRVRSKSGGISTLEIWPWKYLVPWMREDLFSRRDARKKKGPPYLYGNDSYDMKEVVMPLHDYVNHPVAHDLVHNGATFQVAGLTNNSNIREAHELRRCVITHKILGKYVLQVAKKRLDDMLYVGITEDHRESATTFANVVGAQVISQLMASTVNNSNTTEQGPPSSDSSIDATENQDNSSSTTEAKSENMTVGKLMETYETCISSLRSSQSQRRTASLKKVSPANFTKEARRRVPDNALKEIISLNSLDMELYKYAKEIFQKQHRTMEQELHDSVTPVSSHFLNKRRDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DEX4</accession>
    <name>A0AAD5DEX4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">60S ribosomal protein L35a-1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_005605</name>
        <name evidence="4" type="ORF">M8C21_006496</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL33 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008277">
        <property type="protein sequence ID" value="KAI7741094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAMZMK010000356">
        <property type="protein sequence ID" value="KAI7756515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DEX4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.190:FF:000001">
        <property type="entry name" value="60S ribosomal protein L35a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.190">
        <property type="entry name" value="translation elongation factor selb, chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00573">
        <property type="entry name" value="Ribosomal_eL33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001780">
        <property type="entry name" value="Ribosomal_eL33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018266">
        <property type="entry name" value="Ribosomal_eL33_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038661">
        <property type="entry name" value="Ribosomal_eL33_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10902">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L35A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01247">
        <property type="entry name" value="Ribosomal_L35Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01105">
        <property type="entry name" value="RIBOSOMAL_L35AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7741094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7756515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="112" mass="12900" checksum="632A1830EDE6F13D" modified="2024-05-29" version="1">MVKGRQGERVRLYTRGTILGYKRSKSNQYPNTSLVQIEGVNTKEEVAWYQGKRMAYVYKAKVKKNGSHYRCIWGKVTRPHGNTGVVRAKFKSNLPPKSMGSRVRVFMYPSNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G236</accession>
    <name>A0AAD5G236_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">M8C21_026075</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">AA19_3_7</strain>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the RING-type zinc finger family. ATL subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="JAMZMK010011990">
        <property type="protein sequence ID" value="KAI7725277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G236"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030247">
        <property type="term" value="F:polysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16461">
        <property type="entry name" value="RING-H2_EL5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046948">
        <property type="entry name" value="ATL20-22-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025287">
        <property type="entry name" value="WAK_GUB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46279">
        <property type="entry name" value="RING/U-BOX SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46279:SF20">
        <property type="entry name" value="ZINC FINGER, RING_FYVE_PHD-TYPE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13947">
        <property type="entry name" value="GUB_WAK_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 18" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042176067" description="RING-type E3 ubiquitin transferase" evidence="18">
        <location>
            <begin position="23"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="230"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="19">
        <location>
            <begin position="311"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="356"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="358"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00024209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAI7725277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="376" mass="41980" checksum="806500069EA23F86" modified="2024-05-29" version="1">MDNDHILQLVFFSFLLLRSISAADYGCQPASCAPNEPLIRFPFRIIDRQPSGCGFPGFHLYCNKQNRTIVRLPSSRSYIVNRIDYLSQVIYIDQDFCQQNEINDLNLTGTPFELASLHRYTFYNCSVRNFGFMYPATRFSCLNSTNHSVIAVRTELFSPRTMPFGCKVMKTISVSVPFYGEIGSETELLWFNRTCSSCENEGRIRDCGPEDRQSGCLGSSSNGIPTSAKYVLTLGIGVPASICLIGVICYAASKARNYSETETHHQSIDFFSISIIPRPPSRTGLDGATIESYPKTMLGESCRLPNDDKACVICLSDYKAKESLRTIPECNHYFHADCIDEWLRLNATCPVCRNTPQSSSLVTPCSSTSMTSVEPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G2X1</accession>
    <name>A0AAD5G2X1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Wound-induced basic protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_008631</name>
        <name evidence="3" type="ORF">M8C21_014742</name>
        <name evidence="4" type="ORF">M8C21_030734</name>
    </gene>
    <organism evidence="2 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011742">
        <property type="protein sequence ID" value="KAI7726147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAMZMK010010699">
        <property type="protein sequence ID" value="KAI7730622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAMZMK010006751">
        <property type="protein sequence ID" value="KAI7747461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G2X1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012643">
        <property type="entry name" value="Wound_ind"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36752">
        <property type="entry name" value="OS12G0405700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36752:SF2">
        <property type="entry name" value="OS12G0405700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08186">
        <property type="entry name" value="Wound_ind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="12"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7726147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7730622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7747461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="47" mass="5477" checksum="BB940185E845CB8C" modified="2024-05-29" version="1">MIYDVNSPLFRSFLSQKGGSSDKRKTEEQRPKEHKPKANENKPVMNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G4R4</accession>
    <name>A0AAD5G4R4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">PSII 6.1 kDa protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_003815</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast thylakoid membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the psbW family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011356">
        <property type="protein sequence ID" value="KAI7727511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G4R4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009523">
        <property type="term" value="C:photosystem II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015979">
        <property type="term" value="P:photosynthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042549">
        <property type="term" value="P:photosystem II stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009806">
        <property type="entry name" value="PSII_PsbW_class2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34552">
        <property type="entry name" value="PHOTOSYSTEM II REACTION CENTER W PROTEIN, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34552:SF12">
        <property type="entry name" value="PHOTOSYSTEM II REACTION CENTER W PROTEIN, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07123">
        <property type="entry name" value="PsbW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0602">Photosynthesis</keyword>
    <keyword evidence="8" id="KW-0604">Photosystem II</keyword>
    <keyword evidence="5" id="KW-0934">Plastid</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0793">Thylakoid</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="47"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7727511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="125" mass="13354" checksum="265925216FE83F78" modified="2024-05-29" version="1">MASISACTPTLLPNKPSTLLGLPSTYKMGRVQCSIGRETIKDGSSRLVMTSMSSLVATTVVTTMSSPMAMALVDERMSTEGTGLPFGLSNNLLGWILFGVFGLIWTLYFIYTGSLDEDEDSGLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G6G6</accession>
    <name>A0AAD5G6G6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CBS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_022735</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010833">
        <property type="protein sequence ID" value="KAI7730182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G6G6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02859">
        <property type="entry name" value="E_set_AMPKbeta_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032640">
        <property type="entry name" value="AMPK1_CBM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050511">
        <property type="entry name" value="AMPK_gamma/SDS23_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13780">
        <property type="entry name" value="AMP-ACTIVATED PROTEIN KINASE, GAMMA REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13780:SF131">
        <property type="entry name" value="SUCROSE NONFERMENTING 4-LIKE PROTEIN ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16561">
        <property type="entry name" value="AMPK1_CBM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0129">CBS domain</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CBS" evidence="4">
        <location>
            <begin position="153"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="4">
        <location>
            <begin position="325"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7730182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="454" mass="50652" checksum="2E2E145E494D75FA" modified="2024-05-29" version="1">MVHTQFVWSHGGNQVFLCGDFTGRVKYQQMVPVEGSNSTFMTICDLPPGLHKYKFLVDGVWRVDQQQLFKEDEYGVNNIILVKPPEIMTQQLVVDDGLPVMDIDGLDDHHHADSASTSSNIPHQTFLPLRADDIETTRNRLSQHLSSYPVYELIPDSGKVFALDANLSVEEAFLIMHSEGLAVAPLWDQASHQISGMLTASDFILILMELQRNHAMVANHALELSSISAWKEGKLQLHKRPLIKVDPDETLNAVAFRILHNHISSVPVIYVAQDTTFPQLLHVACLSGLLKHVTRHFEHRIGYLPLLQQPIGSLPIGSWIRDIGGARELLTLLSHELLSDALRLLLDEHISSVPIVDTKGTLVNIFSRSDITSLAKGNVYARIQLDHATIAQALQLVDMKAHSRFETCTRTDTLQRVITLLSDPACVSFMERWLCVLPLKRPNGWAWSAACGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G6I2</accession>
    <name>A0AAD5G6I2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_027411</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010784">
        <property type="protein sequence ID" value="KAI7730389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G6I2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7730389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="109" mass="12623" checksum="070482A089DBF41D" modified="2024-05-29" version="1" fragment="single">VIEKENNFKQLGERYRSFPPDRIIGARSDPRVTMENFNDTDTITEIWPALFRLELQKGCKVRDRLQTSSSMVMLSAGCCNGLHTVEQISEMQYVQFMLQKVSFVGNMDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G739</accession>
    <name>A0AAD5G739_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_024758</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010604">
        <property type="protein sequence ID" value="KAI7730872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G739"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904294">
        <property type="term" value="P:positive regulation of ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16532">
        <property type="entry name" value="RING-HC_RNFT1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044235">
        <property type="entry name" value="RNFT1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15860:SF27">
        <property type="entry name" value="RING_U-BOX SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15860">
        <property type="entry name" value="UNCHARACTERIZED RING FINGER-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="142"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="169"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="12">
        <location>
            <begin position="312"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="53"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="56"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7730872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="435" mass="48997" checksum="CF149084E030357F" modified="2024-05-29" version="1" fragment="single">MIRAPLLAVFEHAGVFRHSQGGSGLDNEMQTDEVSIRIMSGVEEEEELSVNTVVDGDGDDDQVDDGEMSLGSSNNNINNNRYDIQNVAQWIEQILPFSLLLLVVLIRQHLQGFFGTIWISAVLFKSNDILQKQTALKGERKFLVLISISMIFMLHVVFVYWWYQNDDLMYPLVLIPPKSVPLFWHALFIILVNDSMARQTAMAFKCLLLMHYKNTGGHNYRKQGQMLTLVEYTLLLYRTLLPTPVWYQFFLNKDDGSIFSSVTTGLYLTFKFTSVIQKVRLLVAALRALSCKELHYGSSASTEQVAAAGDLCAICQEKLRSPIVLRCKHIFCEECVSEWFDRERTCPLCRALVKPADIKSYSDGSTTLKSHGSISSESGCHESMLRISIDLTAGDGTCSDNCPAIGSPDVKQNNLTKKKHLTTCVDRLRLQRRGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G7J8</accession>
    <name>A0AAD5G7J8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Phosphofructokinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_016847</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>beta-D-fructose 6-phosphate + ATP = beta-D-fructose 1,6-bisphosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16109"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010339">
        <property type="protein sequence ID" value="KAI7732010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003872">
        <property type="term" value="F:6-phosphofructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006002">
        <property type="term" value="P:fructose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.450:FF:000002">
        <property type="entry name" value="ATP-dependent 6-phosphofructokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022953">
        <property type="entry name" value="ATP_PFK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050929">
        <property type="entry name" value="PFKA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000023">
        <property type="entry name" value="Phosphofructokinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035966">
        <property type="entry name" value="PKF_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012004">
        <property type="entry name" value="PyroP-dep_PFK_TP0108"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005301">
        <property type="entry name" value="PRK06830.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45770">
        <property type="entry name" value="ATP-DEPENDENT 6-PHOSPHOFRUCTOKINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00365">
        <property type="entry name" value="PFK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000534">
        <property type="entry name" value="PPi_PFK_TP0108"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00476">
        <property type="entry name" value="PHFRCTKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53784">
        <property type="entry name" value="Phosphofructokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphofructokinase" evidence="12">
        <location>
            <begin position="151"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7732010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="498" mass="54760" checksum="7EDCCEB36718C0E6" modified="2024-05-29" version="1">MDLSLSFASSSNLQLSLTKSLSFSTRLHKPLPFFTCNPKIYSTKTSITGYYPVKAQTLNHNNTPPIIDIDDDDGFILEDVPHLTNFLPDLPTYPNPLKNSQAYAIVKNTFVSPEDVLAQQIVVQKGSPRGVHFRRAGPREKVYFKPEEVRACIVTCGGLCPGINTVIREIVCGLNYMYGVKNVLGIEGGYRGFYAKNTKELNPNVVNDIHKRGGTILQTSRGGHDTNKIVDNIQDRGINQVYIIGGDGTQKGAAAIFKEVEKRRLPVAVVGIPKTIDNDIAVIDKSFGFDTAVEEAQRAINAAHVEVESVENGVGXXXXDTVASFIAMFATLASRDVGGLFEFVQRRLKENGHVVIVLAEGAAQDYVSESVNAPEEKDASGNKLLFDVGPWLTQKIKDHFTTVKKMAINMKYIDPTYMIRAVPSNAYDNIYCTLLAQSAVHGAMAGYSGFTVGPVNSRHAYIPIERVTEATNVVKLTDRMWARLLASTNQPTFLDKHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GA46</accession>
    <name>A0AAD5GA46_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_007506</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009774">
        <property type="protein sequence ID" value="KAI7734262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GA46"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45093:SF2">
        <property type="entry name" value="LISH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45093">
        <property type="entry name" value="TRANSCRIPTION ACTIVATOR MSS11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="60"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="75"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7734262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="234" mass="24733" checksum="A0ECB02933F626D0" modified="2024-05-29" version="1" fragment="single">NSNPQQQPHLQHSLSAPLIQNSNLNLQQDKMLGTSSVTADGSMSNSFRGNDQNGRKRKQPVSSSGPANSSGTGNTAGPSPSSAPSTPSTHTPGDVISMPALPHNTNSSKPLMMFGTDGPATLTSPSNQLWDDKDIGQADMDHFVEDGSLDDNVESFLSHDDNDPRDAVGRSMDVSKEFTFTEVRSVRASASKSLELWNMSENKTMTLSAHEGLIAGLALSTVTGLVASASHDKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GAI7</accession>
    <name>A0AAD5GAI7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_002007</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Phosphate:H(+) symporter (TC 2.A.1.9) family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010011">
        <property type="protein sequence ID" value="KAI7733391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GAI7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015149">
        <property type="term" value="F:hexose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17315">
        <property type="entry name" value="MFS_GLUT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045263">
        <property type="entry name" value="GLUT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23503:SF8">
        <property type="entry name" value="FACILITATED GLUCOSE TRANSPORTER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23503">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="80"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="118"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="153"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="177"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="208"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="236"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="319"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="355"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="382"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="411"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="82"/>
            <end position="524"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7733391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="524" mass="56367" checksum="E58C04FDD534ADE0" modified="2024-05-29" version="1">MAFCFCGFSSCGLKEWSKLCNSSSAWIFCAMPALPHGASSMYKRMPSRDGTNSVDLEDDSELLQDDMVQEYSDPPLKHSLPHVLVASIVSFLFGYHLGVVNEPLESISHDLGFNGNSLAEGLIVSTCLGGAFVGSLFSGWIADEFGRRRTFQLCALPMVIGAACSATANSLSGMLLGRFIVGIGLGIGPPVAALYITEVSPPSVRGTYGSFIQISTCLGLLGALLIGIPVKSISGWWRICFWLSTIPSTVLALAMIFCAESPHWLYKRGRNTEAEVEFQKLLGPANVRSAMAELLNSDRVDENDTVSISELIYGRHFRVVFIGSTLFALQQLSGINAIFYFSSTVFRSAGVPSNLANAFVGIVNLVGSFVALLLMDILGRKVLLLWSFFGMAVSTVFQVAAAGLFSSTSGALYLSVGGMLMFVFSFSIGAGPVPGLLLSEIFPNRIRAKAMAFCVQFHGGVTIPAFVGANGPTTVVRDVGYVLFGGGRLCKKKRDGDKRKNTSRNRDCSFATRLGRSLTRLRLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GCW7</accession>
    <name>A0AAD5GCW7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NAB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_013610</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009178">
        <property type="protein sequence ID" value="KAI7736854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GCW7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051861">
        <property type="entry name" value="NET_actin-binding_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32258:SF14">
        <property type="entry name" value="GB|AAF19561.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32258">
        <property type="entry name" value="PROTEIN NETWORKED 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="154"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="194"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="285"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="408"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="162"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7736854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="529" mass="59602" checksum="4EB33F1579CE0256" modified="2024-05-29" version="1">MIYVAGQLVYMEGIESKALFPGEDSQISPGISISLVENVEGFQQCAKEILKLIEQDGNFSETTAETYDDKKSKLSTHIGELLRMHGALVDQHAHLIGEFSKNSSSLIKKQHLDASDSSSPQVTQMFTPDQMSNIQKFRTPIGFDVLSSCGGGSYASRREGSESSFSPSSDSDSESFMSINKLLISPLNGDDALKVKESKVHDDLLQKISNLEEEVVTLNTKIQSLADENTNLKQELQEQELDIKTQNAYLETEKMKGIELEKQVADLKLVISNSNLQTKTMGEELEASRDKLSAAEDEISKLMHELSSKISKLGSTQQDMALLEKQLDCEKENNLALQEEVSAFVADISVRDDQITELNTKINQSTNEISLLKSTHKAKEDNWNADIERLRTELREKCISVDDLNKSLDYLKLKKDGLTAQLDTLQAEKCCQDNLVRELETALNSLQHEHERVLLSSDNAQKVTNELKLKVMELEREVDMQKEVMSDRAEEKREAIRQLSFSLEHYMSGYKQLRQAFVVGNKRHAFFTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GCZ2</accession>
    <name>A0AAD5GCZ2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_029626</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008966">
        <property type="protein sequence ID" value="KAI7737727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GCZ2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34112:SF12">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34112">
        <property type="entry name" value="C-JUN-AMINO-TERMINAL KINASE-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="314"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="347"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="386"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="445"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="123"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="394"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7737727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="569" mass="60712" checksum="A62C263198160309" modified="2024-05-29" version="1">MERSEPTFVPEWLKSSGSLSATSHNSDEQGVLKAKRNKLLENVSDNDLGRPSGSEKTTSSYFRRASSSNGSPYLRSYGSFGRYSDKEKAESRHRDYSDPLGSILPSRFEKEGLRRSHSSLSTKRGDSWPRKVVNDSTVVNKTSHGHSNGVGLRSGGGISSVKTAFERDFPSLGAEEKQDSEIGRVPSPGLSSAIQSLPIGSSAVIGGDGWTSALAEVPVIVGSNGSNTSVVQPVQSTSISATTSMTTGRNMAETLAQGPPKAQTAPQLSVGTQRLEELAVKQSRQLIPVTPSMPKALALSSSDKQKPKVAQAQLQSSHLVNSPRPVSTKLDSTKTSTVGKLHVLKPSRERNGVTPVAKDTLSPTAGGKLPNSPLAVPSVVGPVPLRNNSPVAVPERKPGVVTLEKRPSSQAQSRSDFFNLMRKKSMAPITPENAGSSISASDKFGEAVTEGGSGGTQQSNSENKDDLARNADANVATEISSNNGRNNANSDVILYSEEEEARLLHLMGWEETADEEGLTEEEINSFYRDASKYLNLQAASKIFKGTQPKLLIPLRSQISEISSDSKLES</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GE22</accession>
    <name>A0AAD5GE22_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein FAR1-RELATED SEQUENCE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_013195</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008890">
        <property type="protein sequence ID" value="KAI7738024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GE22"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47718">
        <property type="entry name" value="OS01G0519700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47718:SF12">
        <property type="entry name" value="PROTEIN FAR1-RELATED SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="387"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7738024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="456" mass="50116" checksum="59B4E2EE1E6310B9" modified="2024-05-29" version="1" fragment="single">GKRNLMATNTPNNELTDFLSAEDLELVNRFFLENTQPLSMISPSHGTSKGKGKEVVDEVGSSHEVQSTVVEVGSSHVPDLNNLEEEGVGEDEGLKGSFFPWFERCSSWFTPNGIRYSSPIVPECDKPKVMTTFRQWEQVVDFYEAYAKKCGFSTRLGTMKRKNGSITHRESTFKVCDCKASIRVKFCEKTASWDIYHFEEPHNHMIVPTYNRDLIKLGRRLDFSTKEFIHRVSMNKVGATVAHKIQSIRPFIGERDSQLMIEKLNDRVKNLPNFFFKYSLVNGELRGGNLAAAAFDLPGRATGCPALLQGTSLPLSLDTKSDSLDVGNVDATHNFTYTVTTNVGASEPILTLHFPSSLDLISQHNTPSHYTGIRVSFQKVKAEIMGRGVSSGGGQSSLNYLFGSGEEPKPTTASKGNAEAPPSQDRPSTKVHAAPGGGSSLGYLFGDGMFVVGLDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GER8</accession>
    <name>A0AAD5GER8_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_001259</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">P-body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat EDC4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009022">
        <property type="protein sequence ID" value="KAI7737458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GER8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031087">
        <property type="term" value="P:deadenylation-independent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045152">
        <property type="entry name" value="EDC4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15598">
        <property type="entry name" value="ENHANCER OF MRNA-DECAPPING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15598:SF5">
        <property type="entry name" value="ENHANCER OF MRNA-DECAPPING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7737458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="424" mass="47084" checksum="DDCE635F95ADF499" modified="2024-05-29" version="1">MTSPANNNNHNHNQPPPFDVQRLFNPISSPSPPPPPITTLHNPNNLYSAASYPPPTVTNAPYFHHHHFPNINILNNNNNHHHITNPNPNLNPLHHQLSLPYSNIPIQPPIPNNINNNSHNDITNNNNNANSNPNNHGARLMELLNTPTSSVPDASSQSSSGPMRMLSSKVAKGRHLVGERVVYDVDVRLPGEVQPQLEVTPITKYGSDPNLVVGRQIAVNKSYICYGLKTGTVRVLNINTALRSLLKVFGDSGKLIVIVMFLKLRIVRVTDMCFFAEDVHLLASASMDGRVYVWKILEVPDEDDKPQITEKIAVAVQILGGGEPVHPRVCWHCHKQEVLVVGIGNRVLRFDTTKVGRGKVYSADEPLICHVDKLIDGIQFVGKHDGEITDLSMCQWMTTRLVSASVDGTVSSHRYLLFKLLYRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GEW0</accession>
    <name>A0AAD5GEW0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCAAT-binding factor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_009334</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CBF/MAK21 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008947">
        <property type="protein sequence ID" value="KAI7737831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GEW0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030692">
        <property type="term" value="C:Noc4p-Nop14p complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005612">
        <property type="entry name" value="CCAAT-binding_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027193">
        <property type="entry name" value="Noc4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12455">
        <property type="entry name" value="NUCLEOLAR COMPLEX PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12455:SF0">
        <property type="entry name" value="NUCLEOLAR COMPLEX PROTEIN 4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03914">
        <property type="entry name" value="CBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCAAT-binding factor" evidence="3">
        <location>
            <begin position="347"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="257"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="450"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="257"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7737831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="575" mass="65165" checksum="E4049A68E436BD4C" modified="2024-05-29" version="1">MASPKKRKNNNKQSTQTNKKNKKNQSLADVQKLGQQLLSNRVHINNLPLLINILNPTSFSPPEFILESILSIQSFFTPLVHDLPSSSAKPTEDPESVYKIWLRSKFDEFVQFLIELSVCSTLSEEALREVVVDTLMECVKVGNAGKFHSSVYHKLIHKMVYSIEEADTLLDLLVSKYFNYFTYISLEKLIRPIATKQRSDVRSESADSEKESPAGASEQLLVRNIHDVLSKIPPLQLGEKTEFEMWNASGVFTKDSKMETKKSKGTEEKHGKVKQSSNDVPSSAIISKKMKLKFSKAWMAFLTLSLPIDVYKEVLVTLHQAVIPYLSNPKLLSDFLTESYNIGGVISVMALSSLFILMTEHGLEYPNLYVKLYALLQPSIFIAKHRAKFFQLLDSCLKSPLLPAYLAASFAKKLSRLALTIPPSGSLVVIALIHNLLRRHPSINFLVHQDEKDQTDKDDTSTKPGIDHFNNEETDLSKTKAMRSSLWEIDTLRHHYCPPVSRFVLSLENDLTVRSKTTEVAVKDFSSGSYATIFRDEMRRRVKQVPLAFYKAIPTSLFSESDFGGWTFQCEEQKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GFT1</accession>
    <name>A0AAD5GFT1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Legume lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_033860</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the leguminous lectin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008733">
        <property type="protein sequence ID" value="KAI7738746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GFT1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001220">
        <property type="entry name" value="Legume_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050258">
        <property type="entry name" value="Leguminous_Lectin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32401">
        <property type="entry name" value="CONCANAVALIN A-LIKE LECTIN FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32401:SF51">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00139">
        <property type="entry name" value="Lectin_legB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0430">Lectin</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="Legume lectin" evidence="4">
        <location>
            <begin position="175"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7738746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="298" mass="34244" checksum="BFF18E3E5C9299EA" modified="2024-05-29" version="1">MYDVDIGRRRLLLSDKLGVTVSVGDVESKEINLATGFKFIWVRYFFRGFLSKYLLIEFLFLVIRVMFMSVLAGQATDFEVEQPDIRNGRKDQRREDGESEALTIYITMGQLEIFSTLKLIHLKWPMNRYTRKSHASWSHVKATDSHRPLTPPLGVEQRKKPQSIDASLRRLCGFNLLASNLYVSLPWVKATRMRTEQNIHAWIDFDGPRFEINVTIAPVGVSRSVRPLISFRNLIIRNYLSSEMFVGFSAAKTTWVEAQRVLAWSFSDTGVATTRDPAGLTSQNNELKFRLQAMEQQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GGH7</accession>
    <name>A0AAD5GGH7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">SOSEKI DIX-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_001023</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="8">Homodimer. Forms long polymer filaments with other SOKs proteins polymers (e.g. SOK1, SOK2, SOK3 and SOK4) crucial for polar localization and biological activity. Binds to ANGUSTIFOLIA (AN).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the SOSEKI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008619">
        <property type="protein sequence ID" value="KAI7739481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GGH7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051258">
        <property type="term" value="P:protein polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051302">
        <property type="term" value="P:regulation of cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000067">
        <property type="term" value="P:regulation of root morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090708">
        <property type="term" value="P:specification of plant organ axis polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010369">
        <property type="entry name" value="SOK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048351">
        <property type="entry name" value="SOK_DIX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021182">
        <property type="entry name" value="SOK_magnoliopsida"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31083:SF43">
        <property type="entry name" value="PROTEIN UPSTREAM OF FLC-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31083">
        <property type="entry name" value="UPSTREAM OF FLC PROTEIN (DUF966)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06136">
        <property type="entry name" value="SOK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF031043">
        <property type="entry name" value="UCP031043"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SOSEKI DIX-like" evidence="10">
        <location>
            <begin position="41"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="218"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="308"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="223"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="312"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF06136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7739481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="373" mass="41745" checksum="61F6DAD3A687608D" modified="2024-05-29" version="1">MEIVSKSSMGLHHVSRKWRDQIQRSPERTKVWIEPNKHRRVAVVYYLCRNGQLEHPHFVEVTLSCPEGLYLRDVIDRLNSLRGNGMAALYSWSSKRSYKNGFVWHDLSDNDFIYPVHGQEYILKGSELFDSGNKSDESPSSISKKSSSSETLKSVDQYELLSVNRNSRTNQSWTTIDLHEYKVYSGESTAARIAANASTQTECNNELESESYHDNEISELSRNEISSPPSDSSTETLESLMKSDGKLILRSGTDNEHVDINPTVGSKLKASSVLMQLISCGSISFRNCGPGKQNPGFSLGSKYKHKMLPRGEGVDDRVDKSLGEETGENDVVLGGSEKVNGMEDKEYFSGSLMETKKDEFPALKRSNSYNANG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GI09</accession>
    <name>A0AAD5GI09_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_019501</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HD-ZIP homeobox family. Class II subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008390">
        <property type="protein sequence ID" value="KAI7740623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GI09"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000577">
        <property type="entry name" value="Homeobox-leucine zipper protein 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050762">
        <property type="entry name" value="HD-ZIP_Homeobox_LZ_Class_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006712">
        <property type="entry name" value="HD-ZIP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003106">
        <property type="entry name" value="Leu_zip_homeo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45714">
        <property type="entry name" value="HOMEOBOX-LEUCINE ZIPPER PROTEIN HAT14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45714:SF11">
        <property type="entry name" value="HOMEOBOX-LEUCINE ZIPPER PROTEIN HAT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02183">
        <property type="entry name" value="HALZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04618">
        <property type="entry name" value="HD-ZIP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00340">
        <property type="entry name" value="HALZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 8 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 8 9" id="KW-0371">Homeobox</keyword>
    <keyword evidence="7 8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="12">
        <location>
            <begin position="156"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="8">
        <location>
            <begin position="158"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="16"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="111"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="222"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="113"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="126"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7740623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="331" mass="36539" checksum="59129FCCDC9E7717" modified="2024-05-29" version="1">MAEKDDGLGLSLSLKCSDAVTYPPPPPPPVTGSAGSPRFPLNLLPSPPLSFVQRNVSNHQKMSFMINNNNNNHVDLNPEPRSYQRVIDVNRPPASTSTALVALTVERDDDALVSSPNSTVSSVSGKRVEREDNEAERESSSHGVSGGEDDDDGGDAAAARKKLRLSKDQAAVLEETFKEHNTLNPKQKLALAKQLDLRPRQVEVWFQNRRARTKLKQTEVDCEYLKRCCDTLTEENRRLQKEVNELRALKLSPQFYMNMNPPTTLTMCPQCERVAVSSTSAAATSTAVGSTGLPKALHGSMPANHQKQNNLFLPRASIIPHRPVDAHHPRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GIV9</accession>
    <name>A0AAD5GIV9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_017310</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Binds the phosphorylated C-terminal domain (CTD) of the largest subunit of RNA polymerase II and functions as a scaffold for RNA processing machineries. May be involved in pre-mRNA splicing.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Interacts (via the WW domains) with the phosphorylated C-terminal domain of NRPB1 (via CTD domain).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the PRPF40 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007538">
        <property type="protein sequence ID" value="KAI7744430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GIV9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070063">
        <property type="term" value="F:RNA polymerase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.440:FF:000024">
        <property type="entry name" value="Pre-mRNA-processing protein 40A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.440:FF:000013">
        <property type="entry name" value="pre-mRNA-processing protein 40A isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.440">
        <property type="entry name" value="FF domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002713">
        <property type="entry name" value="FF_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036517">
        <property type="entry name" value="FF_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039726">
        <property type="entry name" value="Prp40-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11864">
        <property type="entry name" value="PRE-MRNA-PROCESSING PROTEIN PRP40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11864:SF0">
        <property type="entry name" value="PRP40 PRE-MRNA PROCESSING FACTOR 40 HOMOLOG A (YEAST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01846">
        <property type="entry name" value="FF"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25432">
        <property type="entry name" value="FF_PRPF40A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00441">
        <property type="entry name" value="FF"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81698">
        <property type="entry name" value="FF domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51676">
        <property type="entry name" value="FF"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="WW" evidence="10">
        <location>
            <begin position="208"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="FF" evidence="11">
        <location>
            <begin position="399"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="domain" description="FF" evidence="11">
        <location>
            <begin position="466"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="domain" description="FF" evidence="11">
        <location>
            <begin position="534"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="domain" description="FF" evidence="11">
        <location>
            <begin position="606"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="260"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="322"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="796"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="11"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="38"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="264"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="322"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="796"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="851"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="873"/>
            <end position="882"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00056384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00061317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00064817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7744430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="888" mass="101245" checksum="0CA63FB876423EA8" modified="2024-05-29" version="1">MANNSQYPGIQQPPRPPVAAMGPPPNTYPLVGMQFRPAGPPRPPPPYMPIVSQQFLAVGRPNMVSQPMQHLPPRPGPPAPTLAHSIVPPPPPPPAAQAFSVPDVQPIRPVISVSPQAQQAVPVPNNYGPGQSGPRAALSLSYNLSTPAPAPAQVHVNAEVTSQYQPKSSLNMPSFPLGGPAVTPMLQPAEQTLVASSVQVTEQPVAVEKAASDWIEHTSHKGKRHVFLPKYYHNKKTKISSWEKPLELMSPTELAREQVITQSDTHEQQSAKDPDNETLIPSVTPGVDSPSSQAHEPASSPVPVPNPISVVQSGSVLTSDSTVSIEASKMTTNVAEETPMPETSPLSAMENVPAKNVAIAENGSIEESNRGTVINDGANNSAAFEEKTVDEETQIYENKQEARNAFKALLESVNVASDWTWDQTMRVIINDRRYGALRSLSERKQAFTEFVGQKKKQEAEQRRAKQIKAREEFKKMLEESKEITVSTKWSKASAIFEDDDRFKAVERFKDREDLYDDYIMELEKKERSKALEEHKKNRKEYVEFLKSCDFLTASSQWRRVQDRLEADERCMRLAKIDRLEIFQEYIRDLEKDEEEQIKLRTEELRKTERKNRDEFRKLMDEHIASGMLTSKSHWRDYCVKVKDLPAYSAVSSNSSGATPKDLFEDVLEELEKQYTVDMDRIKEIVKMRKVSILPTWTLDDFKNTIAEDISSPPVSDLVFDGLLERARDKEEKEAKRRKRVADDFYTSLTTSKEITSSSRWEDVKSLFEDRLEGWFSVEENVFKEMFDKYITELKKARDERKHREDKAKDRDRRTEKSRRDKDNKSKSDKRRRDEARDSHRHSAERKKTKQSEQQSSASAEYESRHKRHKRDHRRDDHDQREAEDGEVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GJ66</accession>
    <name>A0AAD5GJ66_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_020103</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 43 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007811">
        <property type="protein sequence ID" value="KAI7743124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GJ66"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18825">
        <property type="entry name" value="GH43_CtGH43-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.115.10.20">
        <property type="entry name" value="Glycosyl hydrolase domain, family 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006710">
        <property type="entry name" value="Glyco_hydro_43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023296">
        <property type="entry name" value="Glyco_hydro_beta-prop_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22925">
        <property type="entry name" value="GLYCOSYL HYDROLASE 43 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22925:SF3">
        <property type="entry name" value="GLYCOSYL HYDROLASE FAMILY PROTEIN 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04616">
        <property type="entry name" value="Glyco_hydro_43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75005">
        <property type="entry name" value="Arabinanase/levansucrase/invertase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7743124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="470" mass="54326" checksum="8B20625FA847678D" modified="2024-05-29" version="1">MENKMRMKNKYKKPTSLNCYAGSRYRFSLVVCSVIGFVFMFHFYFLNQSQHSQHAVMQMNSEHPLYRELVEVEDDGIKIPPPRKRSPRAIKRKPKRPTTLIEEFLDESSQLRYMFFPGQKTVIDPLTDAGNESLYYHPGRIWLDTDGKPIQAHGGGILYDDWSRTYYWYGEYKDGPTYYAHKKAAARVDIIGVGCYSSKDLWTWKNEGIVLAAEETNETHDLHTSNVLERPKVIYNKNTKKYVMWMHIDDANYTKASVGIAVSDSPTGPFDYIRSTRPHGFESRDMTIFKDDDDVAYLIYSSEDNSELHIGPLTQDYLDVTNVMRRILVGQHREAPSLFKHEGTYYMITSGCTGWAPNEALAHAAESVMGPWETMGNPCVGGNKIFRLATFFAQSTFVLPLHGFPGVFIFMADRWNPADLRDSRYVWLPLLVGGPVDRPLDYSFGFPLWPRVSIYWHKRWKLPYTWSGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GL30</accession>
    <name>A0AAD5GL30_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_010100</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010007717">
        <property type="protein sequence ID" value="KAI7743518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GL30"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05579">
        <property type="entry name" value="STKc_MAST_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000604">
        <property type="entry name" value="AGC protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000340">
        <property type="entry name" value="Serine threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058783">
        <property type="entry name" value="IREH1/IRE-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF415">
        <property type="entry name" value="AGC (CAMP-DEPENDENT, CGMP-DEPENDENT AND PROTEIN KINASE C) KINASE FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26031">
        <property type="entry name" value="IREH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="517"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="12">
        <location>
            <begin position="782"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7743518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="902" mass="100875" checksum="1EDB44240C373FBC" modified="2024-05-29" version="1">MSDMGETTDDDDAEGDNNNDDLNYDLQLEEDYLLHASALGLNHIRTCSVPSPLRIERERERERERLRLEVRDFRRKKVSWSQSKSLRVLPPTIGGLEGNAAISKEMQSPRFQAILRATSGLKKRASDLKSYSHEFNSKGARPLPFSKSRGFGRVEEILVMLRGKFDKLKEEVDTDLGIFAGDLVSILEETSVSHQEWQENLEDLLIIARKCAKMAPSEFWLKCENIVQILDDRRQDLPTGILKQVHTRLLFILTRCTRLVQFHKECGYEEGHHVLGLHQLSDLSVYHKVLESTYQSGSSSLGETEANETQIGHNEEQNQAKLNVDDGVDHVDVSTAKSAAPSTSSFRTSSCMKFSSTVEKNLKGHDEVDTQTKDVSDRLDPKEDQYLTTGIPVCTPGDLEMPSDLRRVPSEASIHKLLEIARSVANINTNEYSALEIMLDHLTDLKFAIQDRKADALENCRKREKYVLLCGQIDDDKIELSSNMANEDSSVDDETVRSLRASPVNTFSKDRTTIEDFEIIKPISRGAFGRVFLAKKRATGDVFAIKVLKKADMIRKNAVESILAERDILISVRNPFVFLNGGDLFSLLRTLGCLEENMARVYIAEVVLALEYLHSLNIIHRDMKPDNLLIGHDGHIKLTDFGLSKVGLINSTEDLSGTSGSRAPVYGENEVTTENGQKQSVVGTPDYLAPEILLGVGHGATADWWSVGVILFELLVGIPPFNAESPQQVFANIMNRDIPWPKVPDEMSYEAYDLINQLLTENPVQRLGATGSAEVKTHPFFRNIHWDTLARQKATFIPSAEALDTSYFMSRYIWNPEDEQVDGGSDFDDMSDTGSTFGDSSYGNMLDEEGDECGNLAEFGASNLNVNYSFSNFSFKNLSQLASINYDVLVKSAQDSIDGSKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GL60</accession>
    <name>A0AAD5GL60_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cysteine synthase</fullName>
            <ecNumber evidence="10">2.5.1.47</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_018116</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate</text>
            <dbReference type="Rhea" id="RHEA:14829"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:58340"/>
            <dbReference type="EC" id="2.5.1.47"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8 10">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the cysteine synthase/cystathionine beta-synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.47" evidence="10"/>
    <dbReference type="EMBL" id="JAMZMK010007553">
        <property type="protein sequence ID" value="KAI7744281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GL60"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004124">
        <property type="term" value="F:cysteine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006535">
        <property type="term" value="P:cysteine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01561">
        <property type="entry name" value="CBS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000006">
        <property type="entry name" value="Cysteine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000130">
        <property type="entry name" value="Cysteine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005856">
        <property type="entry name" value="Cys_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050214">
        <property type="entry name" value="Cys_Synth/Cystath_Beta-Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005859">
        <property type="entry name" value="CysK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001216">
        <property type="entry name" value="P-phosphate_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01139">
        <property type="entry name" value="cysK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01136">
        <property type="entry name" value="cysKM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10314">
        <property type="entry name" value="CYSTATHIONINE BETA-SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00901">
        <property type="entry name" value="CYS_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7 10" id="KW-0198">Cysteine biosynthesis</keyword>
    <keyword evidence="6 8 10" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="11">
        <location>
            <begin position="11"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="183"/>
            <end position="187"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="271"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="9">
        <location>
            <position position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR605856-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR605856-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7744281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="323" mass="34781" checksum="D7BABD75F3C9A68F" modified="2024-05-29" version="1">MGFNVGIAEDVTELIGNTPMVYLKNIVDGCGARIAVKLEAMEPCSSIKDRIALSMIKDAEDKGLITPRKTVLVEGTSGNTGIRLAFIAAIKGYRLIIVMPSISSMERRIVMRSFGAELRITDVSKGIEGIYQKVDEILEKTPNSYFLNQLENPANPKIHYETTGPEIWKASEGKVDAFVSGIGTGGTVTGVGKFLKEKNADIKIYGVEPSESAVLNGGNPGPHKIQGIGNGIIPDVLDVRVLDEVIMITSDEAIETAKLLALKEGLLVGISSGAAAAAAIKIAKRPEYEGKLIVVVFPSFGERYLSTELFDSMRKEVENLPIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GM24</accession>
    <name>A0AAD5GM24_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_028521</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="JAMZMK010006614">
        <property type="protein sequence ID" value="KAI7748017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GM24"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001023">
        <property type="entry name" value="Os07g0541700 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002">
        <property type="entry name" value="RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE SD1-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002:SF548">
        <property type="entry name" value="RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7748017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="82" mass="9694" checksum="350751585461183E" modified="2024-05-29" version="1">MKALLDWPKRWNIIEGIAQGLLYLHKYSRMRVIHRDLKASNILLDENMNPKISDFGMARIFKQDETEAMTKRVVGTLYEHSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GMQ0</accession>
    <name>A0AAD5GMQ0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_012447</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006496">
        <property type="protein sequence ID" value="KAI7748647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GMQ0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009910">
        <property type="term" value="P:negative regulation of flower development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048367">
        <property type="term" value="P:shoot system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034583">
        <property type="entry name" value="EMF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35504">
        <property type="entry name" value="PROTEIN EMBRYONIC FLOWER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35504:SF1">
        <property type="entry name" value="PROTEIN EMBRYONIC FLOWER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="237"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="237"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7748647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="457" mass="51201" checksum="B0181191F6649919" modified="2024-05-29" version="1">MEIDLNKEESTEISIFINFSIREYVAEIRKTNPVKCWPFARDPDNKVVSSYQSSESTFLSGKNCPCNINGSDDTTDITEALNLSKVVNFNGKEDRPETTIDMTESLGSVNSNMGKVVCNGSSKLTLCKVNTGQNRLENNVDEGAKIRHNEAADNNEPGRNQLRRKPYKSRLLSDIYKDPASELRASCDITNAVNVNKRFVAEVDDDLDDDDVTLAAYFRKQKGVDITDAKLNKKRDINRVEEPSVDHDKKSNHGSKGSIGKDSNVATSRMKSRTDDMDTQTMPENKKDFQLQCSPKSNQFAEVSVKEATGKEKENEDSELEAVMLLACHFNEQKQILRELETDTTCARVKKKFGEHPTQSNKKLSIKTPAKTARKHIVKKITNEKLENSFYSVQRMSFMPAKATTDGDFQSHVEMTMMKAAGVGPDAQNCATLVCTFNRNPADFFTPNGEHKFMRGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GQA1</accession>
    <name>A0AAD5GQA1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_012401</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="JAMZMK010006496">
        <property type="protein sequence ID" value="KAI7748601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GQA1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001023">
        <property type="entry name" value="Os07g0541700 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044576">
        <property type="entry name" value="At4g25390-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46821:SF2">
        <property type="entry name" value="OS03G0251700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46821">
        <property type="entry name" value="OS07G0586332 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="32"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="92"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="275"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="417"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="514"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="655"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="281"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7748601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="679" mass="75982" checksum="0A15901F726E4A18" modified="2024-05-29" version="1">MSTLPPTLPQLPTIQPPPAPPYNHRHHHGSTVLHSVLAGALVFLLFVILAIAYRKISRKRTAPADLKSPPPQPKKPLQQFSYKALRRATSAFAASNRLGQGGFGSVYKGVLPSGKEIAVKLMNSTGSLQGEREFHNELSLSTRIAGACGARSGNVISVIGFCSDNWRRKKKMLIVYEYMRNGSLQDALLYRKCPELMDWKIRFMILLDVARGIEFLHCSCDPPIVHCDIKPSNVLLDCNLNAKVADFGLAKVLGLDENEVIETFLECGEEEGDLDTETADDNINNNNNDNNDNNTDVEINKSVAEETESVVTGDVVVNVDPVSPESCRVTVVDLEASPSEYLERTSVSDQISVDSSNRRFLGKKKSGVGGSGRDWWWKQESWGGESGRVKDYVMEWIGSEIKKERPKKDWLTTTTDQFSSELEGSTANGLQKKQKKKAETEKKNRKPREWWKEEFCDELAKKKKKKRRGSSSTGEMWWQRDEEFVPQKKKSKNSSKSSIDWWIDGLSGEFRHRRRNSQECPSGEIPKSGGISSTPSMRGTVCYIAPEKCDGGQLSEKSDVYSYGVLLLVLVSGRRPLQVTASPMSEFERANLISWARQLARNGKLLDLVDPNIHSLDQEQAMLCITTALLCLQRSPIKRPSMREVVGMLSGEADPPHLPFEFSPSPPTNIPFKSRRKPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GQF9</accession>
    <name>A0AAD5GQF9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_013401</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005881">
        <property type="protein sequence ID" value="KAI7751542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GQF9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000009">
        <property type="entry name" value="Glycogen synthase kinase-3 beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050591">
        <property type="entry name" value="GSK-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24057">
        <property type="entry name" value="GLYCOGEN SYNTHASE KINASE-3 ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24057:SF77">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="181"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="221"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="73"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7751542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="293" mass="32696" checksum="71771A5623AFDADA" modified="2024-05-29" version="1" fragment="single">MASASVVPASGVREPSGNAVAAEKLPDEMNDMKIRDDREMEPAIVDGNGAETGHIIVTTIGGRNGQPKQTISYMAERVVGHGSFGVVFQAKCLETGEAVAIKKVLQDKRYKNRELQTMRVLDHPNVVALKHCFFSTTEKEELYLNLVLEYVPETVHRVIRHNNKMNQRMPMIYVKLYSYQIFRALAYIHGSIGVVCIEILIKPQALLQQSKKKKAPRSISVTTIALYSVVTVVMTLVSKIEEGTSNFQVDDEFLDSKKSDVPRYAVFNTSKGLITVELYKEGSPEVVDEFIDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GQN4</accession>
    <name>A0AAD5GQN4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_007596</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010006526">
        <property type="protein sequence ID" value="KAI7748438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GQN4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14137">
        <property type="entry name" value="STKc_GSK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000009">
        <property type="entry name" value="Glycogen synthase kinase-3 beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000082">
        <property type="entry name" value="Shaggy-related protein kinase kappa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050591">
        <property type="entry name" value="GSK-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039192">
        <property type="entry name" value="STKc_GSK3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24057">
        <property type="entry name" value="GLYCOGEN SYNTHASE KINASE-3 ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24057:SF77">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="73"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7748438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="409" mass="46283" checksum="93E7C6B1317E73B0" modified="2024-05-29" version="1">MASASTVPASGVREPSGNIVSAERLPDEMNDMKIKDDKEMEPAIVDGNGAETGHIIVTTIGGKNGQPKQTISYMAERVVGHGSFGVVFQAKCIETGESVAIKKVLQDKRYKNRELQTMRVLDHPNVVALKHCFFSTTEKDELYLNLVLEYVPETVYRMIRYYSKMNQRMPIIYVKLYSYQIFRALAYIHGSIGVCHRDIKPQNLLVNPHTHQVKLCDFGSAKVLVKGESNISYICSRYYRAPELIFGATEYTTAIDIWSAGCVLAELLLGQPLFPGDSGVDQLVEIIKVLGTPTREEIKCMNPNYKEYKFPQIKAHPWHKILHKRTPPEAVDLVSRLLQYSPNLRSTALEAMIHPFFDELRDPAVRLPNGRFLPPLFNFKSHELKGVPVEMLKKLVPEHARKHCAFLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GRD4</accession>
    <name>A0AAD5GRD4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_023379</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006297">
        <property type="protein sequence ID" value="KAI7749711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GRD4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009873">
        <property type="term" value="P:ethylene-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00018">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.730.10:FF:000001">
        <property type="entry name" value="Ethylene-responsive transcription factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.730.10">
        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001471">
        <property type="entry name" value="AP2/ERF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31677">
        <property type="entry name" value="AP2 DOMAIN CLASS TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31677:SF49">
        <property type="entry name" value="ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR ERF086"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00847">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00367">
        <property type="entry name" value="ETHRSPELEMNT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00380">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51032">
        <property type="entry name" value="AP2_ERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0936">Ethylene signaling pathway</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0611">Plant defense</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="9">
        <location>
            <begin position="49"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="27"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7749711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="320" mass="35818" checksum="C5F47BEBD5B317F9" modified="2024-05-29" version="1">MSTPKSSSKLKSLENSPHIDMGFTILQRNTSIPQPTERRGRKKEAEPGRFLGVRRRPWGRYAAEIRDPTTKERHWLGTFDTAQEAALAYDRAALSMKGTQARTNFIYSTDHDNTNFHSLISPIDVQTLFPPTQLFNAPPVLPQNPTFNPQHPNQSGVDTCYQSSNDDVHHDFMISEGDNSGYLGCIVPDDYLNPRNNNNENTSHDSYYKHDNNHDNNPRVECSFWDQSSQLFGSNEGELYGDHMWGGSESSWETMNGYDLSAVVNYPTPLSMADDDGHGLYEPSSSYEFMVGGSSNVTNSSSRTSCSYSDIDIVGVGYSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GTP4</accession>
    <name>A0AAD5GTP4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ribose-phosphate diphosphokinase</fullName>
            <ecNumber evidence="2">2.7.6.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_020843</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>D-ribose 5-phosphate + ATP = 5-phospho-alpha-D-ribose 1-diphosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15609"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.6.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the ribose-phosphate pyrophosphokinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.6.1" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010002408">
        <property type="protein sequence ID" value="KAI7754840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GTP4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002189">
        <property type="term" value="C:ribose phosphate diphosphokinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004749">
        <property type="term" value="F:ribose phosphate diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006015">
        <property type="term" value="P:5-phosphoribose 1-diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000032">
        <property type="entry name" value="ribose-phosphate pyrophosphokinase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000034">
        <property type="entry name" value="Ribose-phosphate pyrophosphokinase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029099">
        <property type="entry name" value="Pribosyltran_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005946">
        <property type="entry name" value="Rib-P_diPkinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01251">
        <property type="entry name" value="ribP_PPkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210">
        <property type="entry name" value="RIBOSE-PHOSPHATE DIPHOSPHOKINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210:SF34">
        <property type="entry name" value="RIBOSE-PHOSPHATE PYROPHOSPHOKINASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13793">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01400">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 11" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ribose-phosphate pyrophosphokinase N-terminal" evidence="13">
        <location>
            <begin position="10"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="12">
        <location>
            <begin position="157"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF13793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7754840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="319" mass="35879" checksum="06D63142BB701D58" modified="2024-05-29" version="1">MEKPNKKQVLLFHCVESEELARKVSQQSDLIKLQSINWRSFDDGFPNLFINNAKEIRGQHVAFLASFSSPAVIFEQLSVIFALPRLFVASFTLVLPFFPTGSFERMEEEGDVATAFTMARILSNIPVSRGGPTSLVIYDIHALQERFYFSDHVLPCFETGMPLLKQRLHQLPDADNVVIAFPDDGAWKRFHKMLDHFPTVVCAKVREGDKRIVRLKEGDPAGRHVVIVDDLVQSGGTLIECQKVLAAHGANKVSAYVTHAVFPKKSWERFVHKDDENSKKAFEYFWITDSCANTVKEIANKAPFEVISLAGSIADALQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GVZ2</accession>
    <name>A0AAD5GVZ2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Carotenoid 9,10(9',10')-cleavage dioxygenase 1-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_018437</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="5">Binds 1 Fe(2+) ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the carotenoid oxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000118">
        <property type="protein sequence ID" value="KAI7757450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GVZ2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009570">
        <property type="term" value="C:chloroplast stroma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010436">
        <property type="term" value="F:carotenoid dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016121">
        <property type="term" value="P:carotene catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004294">
        <property type="entry name" value="Carotenoid_Oase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10543">
        <property type="entry name" value="BETA-CAROTENE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10543:SF142">
        <property type="entry name" value="OS06G0162550 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03055">
        <property type="entry name" value="RPE65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="4 5" id="KW-0408">Iron</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="5">
        <location>
            <position position="262"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="311"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="375"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="582"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR604294-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7757450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="590" mass="66581" checksum="06C3409197481C8F" modified="2024-05-29" version="1">MAACNIKAIHVNCCAQRPSSVTSFEQFKNQLLSSFKPFIVDLQKVPSKLLVPHAIIKKTSLDLLHTLTDSVFQFIDHESFPSQSNFGPVEEIGEAIYINDVQGTIPSGFPEGVYIRNGPNPLFGGFKSTNSIFGRSSHIWVEGEGMLHTLYFKKESDGKWSVSYKNKHVETETLKMEKQRNTPSFLPAIEGDSPAILSAYLLNFLRFGTVNKLLSNTSVFEHSGKFYAAAENHLPQEIDIRTLNTLRNWNVNGSWNRPFTAHPKKVPGSGELVMMGVDAMKPFFEIGIISEDGSKLVHRADLKFERCSLCHDIGVTMRYNVILDFPLTIDIKRLVNGGPLIKYDKVDYARIGVMPRYGDADSVRWFQVEPCCVFHIINTYEDGDEVILWAFRAAESIIPGPDFGLNKFDWFSSRFKNECDTSFDSDDSFFSRAYEWRLNMKTGEVKERFLTGKSDSMDFPMINENFTGLKNKYGYAQIVDMDASSFAGMPKYGGLAKLYLEESTQQDYVKMEYHKFPTNTFCTGAAFVAKPGGIEEDDGWVITFVHDEHLNISRAIIVDAKNFTSEPVAIITLPSRVPYGFHGAFMSIML</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GWH6</accession>
    <name>A0AAD5GWH6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_003473</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000906">
        <property type="protein sequence ID" value="KAI7755664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GWH6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7755664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="107" mass="12676" checksum="088BB8C8E51E1901" modified="2024-05-29" version="1" fragment="single">CFNHQKLFDRRKLFQSLDASGEWLYSIHTQLIIKLFWIFTRSITKINVQIWCFLQVSSSKTGSWLINYNHTKFVGYPPRLVGSYDFHNVKQVFEVTVQVVLHPLEAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-06-18" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GXC5</accession>
    <name>A0AAD5GXC5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_033565</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the major facilitator superfamily. Phosphate:H(+) symporter (TC 2.A.1.9) family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000903">
        <property type="protein sequence ID" value="KAI7755681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015145">
        <property type="term" value="F:monosaccharide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17361">
        <property type="entry name" value="MFS_STP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000002">
        <property type="entry name" value="Sugar transport protein 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044778">
        <property type="entry name" value="MFS_STP/MST-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045262">
        <property type="entry name" value="STP/PLT_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23500">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 2, FACILITATED GLUCOSE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23500:SF511">
        <property type="entry name" value="SUGAR TRANSPORT PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="6" id="KW-0769">Symport</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="80"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="110"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="135"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="169"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="196"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="281"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="317"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="346"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="382"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="418"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="445"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="12">
        <location>
            <begin position="26"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7755681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="505" mass="55942" checksum="F5CBBB6CD22BBF02" modified="2024-05-29" version="1">MPAQVISANGGAFDVPAKLTAQVLVCSIIAAFGGLMFGYDIGISGGVTSMDVFLKKFFPHVYVKKHHVKEDNYCKFDDQLLQLFTSSLYLAAVVSSFAASKCCKNWGRKITIQLASFFFLLGVIXXXXAKNIAMLIIGRLLLGCGIGFGNQAVPLFISEIAPTKYRGGLNVCFQLLVTVGILAANIVNYFTASHPYGWRISLGGAAVPALFLAFGSLLIVETPTSLIERGYPELGLETLRKIRGVHDVQKEFDDIVNTTELAKKIKHPFKNLMKRSSLPQLITSGILQIFQQFTGINVIMFYAPVLFQTMGFHSDASLLSAVVTGTINTVATLVAVFCVDKLGRRFLLIEAAIQMFIAQVVTGGILAAFLKSTNTLPKQYAYLVLVLICVFVSGFAWSWGPLGWLIPSEIFPLETRTAGFFCAVSMNMIFTFIIAQAFLTMLCHMRAMIFFFFSGWILLMGLYAYALLPETKGVSIDEINDKVWKKHWLWKRCFDQDHDQTYDKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GXF7</accession>
    <name>A0AAD5GXF7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_009874</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000341">
        <property type="protein sequence ID" value="KAI7756564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GXF7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7756564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="54" mass="6174" checksum="4BD9A3C84FC1B3B3" modified="2024-05-29" version="1">MEPEKTQGCFFPILNVAGIKCSHKKLQQQCIKYPSPIERRLMSLSWKTGSSCMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GXS7</accession>
    <name>A0AAD5GXS7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">histidine kinase</fullName>
            <ecNumber evidence="3">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">M8C21_032296</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">AA19_3_7</strain>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010000623">
        <property type="protein sequence ID" value="KAI7756041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GXS7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009884">
        <property type="term" value="F:cytokinin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043424">
        <property type="term" value="F:protein histidine kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071215">
        <property type="term" value="P:cellular response to abscisic acid stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070417">
        <property type="term" value="P:cellular response to cold"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034757">
        <property type="term" value="P:negative regulation of iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010087">
        <property type="term" value="P:phloem or xylem histogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010271">
        <property type="term" value="P:regulation of chlorophyll catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009909">
        <property type="term" value="P:regulation of flower development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048509">
        <property type="term" value="P:regulation of meristem development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010029">
        <property type="term" value="P:regulation of seed germination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009651">
        <property type="term" value="P:response to salt stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009414">
        <property type="term" value="P:response to water deprivation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080117">
        <property type="term" value="P:secondary growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd16922">
        <property type="entry name" value="HATPase_EvgS-ArcB-TorS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000137">
        <property type="entry name" value="Histidine kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.130:FF:000015">
        <property type="entry name" value="Histidine kinase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.350:FF:000001">
        <property type="entry name" value="Histidine kinase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.350">
        <property type="entry name" value="CHASE domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050956">
        <property type="entry name" value="2C_system_His_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006189">
        <property type="entry name" value="CHASE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042240">
        <property type="entry name" value="CHASE_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056839">
        <property type="entry name" value="Receiver_AHK4/CRE1_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719:SF51">
        <property type="entry name" value="HISTIDINE KINASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03924">
        <property type="entry name" value="CHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24896">
        <property type="entry name" value="Receiver_CRE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01079">
        <property type="entry name" value="CHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50839">
        <property type="entry name" value="CHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 10" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="260"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="CHASE" evidence="15">
        <location>
            <begin position="35"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="13">
        <location>
            <begin position="316"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="14">
        <location>
            <begin position="626"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="14">
        <location>
            <begin position="772"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="75"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="472"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="475"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="10">
        <location>
            <position position="822"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAI7756041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="919" mass="101486" checksum="A4D8BBD78B24FB90" modified="2024-05-29" version="1">MCDQRARMLQDQFSVCVNHVHALAVLVSTFHHYKNPSVIVQDTFAEYTARTAFERPLLSGVAYAPRVLNSEREDFERQHGGTIRTMANKEPSPHRDEYAPVIFSQETVSYLNLLDMMSGEEDRENILRARSTGKAVLTSPFKLLGSHHLGVVLTIPVYKSKLPPNASVRDRIEATAGYLGGAFDVESLVENLLGQLAGNQEIIVKVYDVTNTSDHLIMYGRQTQEGDVTREHVSRLDFGDPYRKHHMTCRYLHKAPISRTAITTAVLGYVIVILAGYMFYTGAIHIVQVEDDFDTMQQLKVRAEAADVAKSRFLATVSHEIRTPMNGILGIILDLRMLALLLDTDLSSTQRDYAQTAEACGRALITLINEVLDRAKIEAGKLELEAVPFDLRSILDDVLSLFSEKSRNKGIELAVLVSDKVPQIVMGDPGRFRQVITNLVGNSVKFTEQGHIFVQVHLAEHSKAVQDAKFESCMNGRSNGPQSKTLSGREAADDRNNWDRFKHLIADEESPFQVLTSDGNSQTVNLMVSVEDTGIGIPLHAQELVFMPFMQADSSTSRHYGGTGIGLSISKCLVELMGGQISFVSRPQIGSTFSFTAKFTRCEKNTVADLKRSLYDDLPTSFKGLKATIVDTKPVRAAVTRYHLKRLGVLVEVVNSIKTAVTGSGSCSCSGQPDMILVEKDVWLSGDDLSNIQVFGRALKSPKLILLATNVTSGELDKAKAAGFSDTLIMKPLRASMVAACLQQVLGTGKAEQGKVMANGSVYLRGLLREKKILVVDDNRVNRRVAAGALKKFGAEVECADSGKAALDLLKIPHEFDACFMDIQMPEMDGFEATRRIRMMESQVNEQQTSGSMDGCCDKQAQARKWHLPILAMTADVIQATFQECQKSGMDGYVSKPFQEENLYQEVAKFFESKPATDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GZU4</accession>
    <name>A0AAD5GZU4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_018753</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000016">
        <property type="protein sequence ID" value="KAI7758231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GZU4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010333">
        <property type="term" value="F:terpene synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016102">
        <property type="term" value="P:diterpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046246">
        <property type="term" value="P:terpene biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00684">
        <property type="entry name" value="Terpene_cyclase_plant_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.130:FF:000001">
        <property type="entry name" value="Isoprene synthase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.130">
        <property type="entry name" value="Terpene synthase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044814">
        <property type="entry name" value="Terpene_cyclase_plant_C1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001906">
        <property type="entry name" value="Terpene_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036965">
        <property type="entry name" value="Terpene_synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050148">
        <property type="entry name" value="Terpene_synthase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005630">
        <property type="entry name" value="Terpene_synthase_metal-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31225">
        <property type="entry name" value="OS04G0344100 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31225:SF196">
        <property type="entry name" value="TERPENOID CYCLASES_PROTEIN PRENYLTRANSFERASE ALPHA-ALPHA TOROID-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01397">
        <property type="entry name" value="Terpene_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03936">
        <property type="entry name" value="Terpene_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Terpene synthase N-terminal" evidence="5">
        <location>
            <begin position="19"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="Terpene synthase metal-binding" evidence="6">
        <location>
            <begin position="224"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7758231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="471" mass="55544" checksum="F4DEB4FA0A84A722" modified="2024-05-29" version="1">MDAKQDEIIRPLANFHPHLWGDQFLVYDEQEDQQEEVDRIIESLKLEVRKEILVALDATEHTSLLKLVDAIQRLGIAYYFEKEIDQALQHIYNLYGDEWNGGSPSLWFRLLRDNGYYVSSDCFNYYKDNGGSFKQSLTNDVEGLLDLYEAAYFRVQGEVILDDALVFTKTHLENIAKDPRRCNSTISRHIQDALERPIRKRLPRLGAIRYIPFYEQQIGRKLLLGVYFEPQYARSRTFLGKMCAMATIMDDTYDAYGTYEELKIFTEAVQTYVTHVYIKQTFFRVLEVVIFRFKCKFIIHLNINYRWSATCMDSLPDYMKPIYKGLLDVFEDMEETLAKEGRASEVNYSKDSMKELNKSYMMEAKWRHEGYGRKEDKQRNHVASTVDSYMKQHDTKEEQVFDLLNLQVEDAWKEINQELLVCKDIPLRLIMNVINLARVIDTLYKHDDNFTRVGEEFKDNIKSCFVHAMNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5H007</accession>
    <name>A0AAD5H007_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">STAS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_019300</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000093">
        <property type="protein sequence ID" value="KAI7757648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5H007"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07042">
        <property type="entry name" value="STAS_SulP_like_sulfate_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.750.24:FF:000002">
        <property type="entry name" value="Sulfate transporter 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.24">
        <property type="entry name" value="STAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011547">
        <property type="entry name" value="SLC26A/SulP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001902">
        <property type="entry name" value="SLC26A/SulP_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11814">
        <property type="entry name" value="SULFATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01740">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00916">
        <property type="entry name" value="Sulfate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52091">
        <property type="entry name" value="SpoIIaa-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50801">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="49"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="83"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="111"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="141"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="STAS" evidence="7">
        <location>
            <begin position="197"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7757648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="324" mass="35247" checksum="1BA04BFBABD4CF12" modified="2024-05-29" version="1">MLHFHGSHIGLVLKTGLVTGIISLTEGIVVGRTFAALKNYQVDGNKEMIAIGVMNVVGSTTSCYVTTGAFSRSAVNHNAGAKTAVSNIIMAVTVMVTLLFLMPLFRYTPNLVLGAIIVTAVIGLIDIPAAYQIWKVDKFDFIVMLCAFFGVIFISVQEGLALAVGISIFKMVLQMTRPKTVVLGNIPGTDIFRNKHQYKEAVTIPGFLILSIQAPISFANSNYLNERILRWILDYEEEEAAKSHSNLRFVILDLAAVSAIDTNGVAFFQELRRLVLVNPVGEVVEKLQNADGTNDLLQPDNLYLTVGEAVSSLFSSMKVVSSSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BL21</accession>
    <name>A0AAD5BL21_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Translocase of chloroplast</fullName>
            <ecNumber evidence="14">3.6.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">M8C21_026063</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">AA19_3_7</strain>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">GTPase involved in protein precursor import into chloroplasts. Seems to recognize chloroplast-destined precursor proteins and regulate their presentation to the translocation channel through GTP hydrolysis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12 14">Plastid</location>
            <location evidence="12 14">Chloroplast outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13 14">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. AIG1/Toc34/Toc159-like paraseptin GTPase family. TOC34 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.-" evidence="14"/>
    <dbReference type="EMBL" id="JAMZMK010011990">
        <property type="protein sequence ID" value="KAI7725265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BL21"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009707">
        <property type="term" value="C:chloroplast outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015450">
        <property type="term" value="F:protein-transporting ATPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01853">
        <property type="entry name" value="Toc34_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001070">
        <property type="entry name" value="Translocase of chloroplast"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006703">
        <property type="entry name" value="G_AIG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045058">
        <property type="entry name" value="GIMA/IAN/Toc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005688">
        <property type="entry name" value="Toc34"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00991">
        <property type="entry name" value="3a0901s02IAP34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903">
        <property type="entry name" value="GTPASE, IMAP FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903:SF156">
        <property type="entry name" value="TRANSLOCASE OF CHLOROPLAST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04548">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038134">
        <property type="entry name" value="Toc34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51720">
        <property type="entry name" value="G_AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 14" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="10 14" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="16" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 14 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 14" id="KW-0934">Plastid</keyword>
    <keyword evidence="7 14" id="KW-1002">Plastid outer membrane</keyword>
    <keyword evidence="8 14" id="KW-0653">Protein transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 14" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AIG1-type G" evidence="17">
        <location>
            <begin position="31"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="43"/>
            <end position="48"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="205"/>
            <end position="206"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00060807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR038134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR038134-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR038134-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAI7725265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="297" mass="33154" checksum="170E2BED02FCC896" modified="2024-05-29" version="1">MAREWTGIQQFPLATQTKLLELLGTLKEKARDSLTILVMGKGGVGKSSTVNSILGERVAAVSAFQSDVPRPVMVSRERSGFTLNIIDTPGIVEGGYVNDQALQLIKRFLLNKTIDVLLYVDRLDSYRVDNLDRQIVNAITESFGKEIWRKGVVVLTHAQLTPPDCLSYDDFVARRSEAVLKVVDSGARLKKHGIQDHVIPVVLVENSNRCKQNDNGEKILPNGTVWIPNIVEIITNVATNESKSIFVDQKLIDGPNANERGKAFIPLILAFQYFFVIKQLQKWIKDDIAKAPKSSWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BL30</accession>
    <name>A0AAD5BL30_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ABC-type xenobiotic transporter</fullName>
            <ecNumber evidence="3">7.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">M8C21_011147</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">AA19_3_7</strain>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O + xenobioticSide 1 = ADP + phosphate + xenobioticSide 2.</text>
            <dbReference type="EC" id="7.6.2.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.2" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010011989">
        <property type="protein sequence ID" value="KAI7725280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008559">
        <property type="term" value="F:ABC-type xenobiotic transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18579">
        <property type="entry name" value="ABC_6TM_ABCC_D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18580">
        <property type="entry name" value="ABC_6TM_ABCC_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03244">
        <property type="entry name" value="ABCC_MRP_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000003">
        <property type="entry name" value="ABC transporter C family member 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000169">
        <property type="entry name" value="ABC transporter C family member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001048">
        <property type="entry name" value="ABC transporter C family member 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000002">
        <property type="entry name" value="ABC transporter C family member 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044746">
        <property type="entry name" value="ABCC_6TM_D1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044726">
        <property type="entry name" value="ABCC_6TM_D2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF362">
        <property type="entry name" value="ABC TRANSPORTER C FAMILY MEMBER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="38"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="93"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="126"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="156"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="195"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="330"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="369"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="449"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="473"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="542"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="574"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="941"/>
            <end position="962"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="991"/>
            <end position="1015"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1058"/>
            <end position="1078"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1084"/>
            <end position="1102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1178"/>
            <end position="1198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1210"/>
            <end position="1228"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="16">
        <location>
            <begin position="332"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="15">
        <location>
            <begin position="646"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="16">
        <location>
            <begin position="991"/>
            <end position="1230"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="15">
        <location>
            <begin position="1267"/>
            <end position="1501"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="878"/>
            <end position="917"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="878"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="901"/>
            <end position="913"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAI7725280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="1509" mass="168318" checksum="5CED8419F4220816" modified="2024-05-29" version="1">MTSVSCSSNSFVASNASGASFVLQWLTFIFLSPCPQRAILSAVDVVFLLVLIVFSIQKLFSRFSSRNNGISAVDEPLITKRTTGVTTSFRFKASVIVTAVLTVCYSVASVLAFTRDTEMQWKLIDGICWLLQAFCFFVIVVLIIHEKRFQAVTHPLSLRVFWVVNFVVIALLASSGVVRLVSGSADASIIESDDIISLISIPFSVFLVFVSVTGSTGILVVSESEPLNGNSNGNSNSNGNVEVLKDYELNKSSVSGWASASILSKVFWLWMNPLLRKGYRAPLKLEDIPTLSPEHRAEKMSKLFEENWPKPHENSKHPVRLALIRCFWKHIAFTALLAVIRLCVMYVGPLLIQRFVDFTSGKRSSPYEGYYLILILLVAKFVEVLSSHQFNFNSQKLGMLIRSTLITSLYKKGLRLSCSARQSHGVGQIVNYMAVDAQQLGDMMLQLHAIWLMPVQVTVALSILYMYLGVPSLVALAGILAVLLYVIFGTKRNNKFQFNIMKNRDSRMKATNEMLNYMRVIKFQAWEEHFNKRIQAFRESEYGWLTKFMFSIGGNLIVLWSAPLFVSSLTFGSAILLGIPLDAGTVFTATSLFKNLQEPIRTFPQSMISLSQAMISLGRLDAFMLSKELNEGAVERQEGCSGNTAVEVKDGSFSWDDEAADGAVVKNLNFEIKKGELSAIVGTVGSGKSSLLSAVIGEMHKISGKVRVCGSTAYVAQTAWIQNGTIQENILFGLPMDRQKYKEVIKNCCLEKDLEMMEFGDQTEIGERGINLSGGQKQRIQLARAVYQDCDIYLLDDVFSAVDAHTGSEIFKECVRGALRNKTILLVTHQVDFLHNVDLILVMRDGMIVQSGKYDELLESGLDFKNLVSAHETSMQLVEMEPTTSDSASPRPLQKSPSLNPREEEQTPLERSKSSSIIGTSKLIEEEERETGRISLGVYKVYVTEAFGWWGVSAVLTFSILWQGSQMASDYWLAYETADERTFNPSLFIEVYTAIAGLSLLLVFGRIVSSTYLGLKTSQIFFKQILYSILHAPMSFFDTTPSGRILSRASSDQTNLDVFLPFMMNLTLAMFITVFGIIIITCQYAWPTVFLLIPLGWLNFWFRGYYLATSREITRLDSITKAPVIHHFSESISGVMTIRCFRKQDRFVQENVDRVNGNLRMDFHNNGSNGWLGFRLEFLGSLFLCASTVFMIILPSSIVKPENVGLSLSYGLSLNGVMFWALYMSCFVENRMVSVERIKQFTNIPSEAEWVKKDNPPPSNWPSHGNVELKDLQVRYRSNTPLVIKGITLSIKGGEKIGVVGRTGGGKSTLIQVLFRLVEPSGGSIIIDGINISTLGLHDLRSRFGIIPQEPILFEGTVRSNIDPIGQHSDEEIWRSLERCQLNDVVAAKPGKLDSAVVDNGDNWSVGQRQLLCLGRVMLKHSKLLFMDEATASVDSQTDAVIQRIIREDFADCTIISIAHRIPTVMDCDRVLVIDAGYAKEFDKPSRLIERPSLFGALVQEYANRSSGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BLJ8</accession>
    <name>A0AAD5BLJ8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">histone acetyltransferase</fullName>
            <ecNumber evidence="3">2.3.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">M8C21_016865</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">AA19_3_7</strain>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Acetyltransferase enzyme. Acetylates histones, giving a specific tag for transcriptional activation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-lysyl-[protein] + acetyl-CoA = N(6)-acetyl-L-lysyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45948"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010012225">
        <property type="protein sequence ID" value="KAI7724414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BLJ8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000123">
        <property type="term" value="C:histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004402">
        <property type="term" value="F:histone acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1020.10">
        <property type="entry name" value="TAZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013178">
        <property type="entry name" value="Histone_AcTrfase_Rtt109/CBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035898">
        <property type="entry name" value="TAZ_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000197">
        <property type="entry name" value="Znf_TAZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13808">
        <property type="entry name" value="CBP/P300-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13808:SF1">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02135">
        <property type="entry name" value="zf-TAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00551">
        <property type="entry name" value="ZnF_TAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57933">
        <property type="entry name" value="TAZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50134">
        <property type="entry name" value="ZF_TAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TAZ-type" evidence="14">
        <location>
            <begin position="572"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="428"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="490"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="528"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="752"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="428"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="438"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="490"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="528"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="15">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAI7724414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="938" mass="103448" checksum="94E44BDF176C2CC5" modified="2024-05-29" version="1" fragment="single">MNLPTHITGQFSGQVPNQSGTSLSAVPHQQNIMQSSGGQRSTLNMEPGFGNARRFIQEKIYEFLMRRQQTQEINPKKMIDIVRRLEEGLFKTATTKEEYMNIETLENRLHVLIKRLPYSNHNQQYQQQGNTSVPMGTMIPNPGMPQSGNSSMMVPTSMDTMVPPAVNTGNFMPNNTRIPSGMHNGSLSSSDGMTNGYQQSTTNFSNNLGGNSLISSMGAQRMASQMIPTPGFGNSDSTSNQSYMNMDSSNNLVGLSTIDSTTVSQPLQQKQQVGGQNSRILHTLGSHMGGGIRSNSHKNAYGLPNRSMNSGLGTMGNQLPIMNGSGTSESYVTASNYGSTAVDSSRSGNVYVPPNSATSIMNNQDMNSVSLHALHKTTSPMMVNHSNLVNTQQGSNMKPLIDQSDKMDFRSQHSLQKPVHFQPQLVQNQNQQTQQYQHLSYGQSQLTPDLGSQSHVSEHFQSSQTVNHFQENFGEGQFLVDTKNGLSSSVGVQPGSVMQGQWHSRSQEMPHVPGNMLEQNVKEEFNQRISGQDQAQRNNLSSEGSITNQSAANKSIDPPNSGANSKSVNLNRELQFKNQQRWLLFLRHARKCAHPPGKCPEVNCITAQKLLNHIASCNDLVQCQYPRCRSTKRLLHHHRHCRDPSCPVCVPVKRFVLLKGAPREEPSQYNMKTSPSVVETSQDLNPALKRMKIEQSSQSVATESQTPSTIVPVISVSQPQDEIGDTSTNTTPTKSKVKGVKLEVLASSVPGSPKKDYLENSGNQKFKGDPVVPTKDASLPKQELVKTEIEVAQVQPKPEKAALPAEASSGTKSGKANIKGVSMIELFTPKQVREHISGLRQWVGQWVQCDKCEMWQHQICALFNGRRNDGGQAEYTCPNCYVEEVERGERSPLPQSAVLGAKDLPRTILSDHIESRLFGKLKQERLERARAHGKSYDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BLM3</accession>
    <name>A0AAD5BLM3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_019256</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011897">
        <property type="protein sequence ID" value="KAI7725580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BLM3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31033:SF18">
        <property type="entry name" value="OS06G0115800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31033">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="211"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="235"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="259"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="118"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7725580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="347" mass="37714" checksum="5B514AD7238FDF5D" modified="2024-05-29" version="1">MDNTSYCPFNQQDIQRCPFLRNINKTTNFSFASIHLPVPENGTRGPIFEDDSNFDMAFKLFHGKDGIVPLSERDSEKAKPESEPKFNPLASNVATISLSAFGPGGPFSFGAFSEKRKMQKCESKSSKNESSRKGDTSTHEALGNEWLVNGNCPIAKSYRAVSKVLPLVASALKLPPGVKLKCPPAVVAARAALARTALVKNLRPQPLPEKMLVIGILGMAANIPLGIWREHTSKFSLSWFIAVHAAVPFIAMLRKSVLMPKTAMAFTIAASILGQVIGSRAERFRLMRTQTQMLAQVVTVETIAGHGSTDVDDLTKGQCRHDEPIGGQFSGKSVRYQSSSSSPGVCY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BPA2</accession>
    <name>A0AAD5BPA2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_021033</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011443">
        <property type="protein sequence ID" value="KAI7727098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BPA2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31150">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31150:SF26">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23413">
        <property type="entry name" value="zf_RING_Vps8_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="3">
        <location>
            <begin position="258"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="90"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="90"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="129"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="160"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="202"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7727098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="422" mass="46721" checksum="4EE86D9019A1B88B" modified="2024-05-29" version="1">MGSACCVAARDRTITNGSNSDVIPRNIRYSPSWSVRWENRRRVAGEEASINWFSDGVVTNDRLDNKSQTTVGTAYASEEGSPLESFRNMTWQKSSPSEGFTVPQSDQSVSQITSEVKDPNETQLASEQSPTMISSTHSVSSFSPSPLSSSQPHFLPPSRWHGRSPGHHLLRQVSDSRIPAMKSPNLSISEEGGSPFMLPGWSNESNRGSHGGSSDSWSVPAFNELMATSHSRRERWSFDSSRTSGRVSSSPSVDMRTCGVCSKLLTERSSWAAQKIIASNELAVVAILICGHVYHAECLENMTSEINKYDPACPVCTFGEKKVIKLSEKALKADMELKARIHKKSRSRVVDGDMNGEDIMFDHHHHFHHKNSPKMGSSSSMKSSVGKPFLKRHFSFGSKGRSLSDNNFPRKKGFFWARSSKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BPK5</accession>
    <name>A0AAD5BPK5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Probable cytosolic iron-sulfur protein assembly protein CIAO1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_014933</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Essential component of the cytosolic iron-sulfur (Fe/S) protein assembly machinery. Required for the maturation of extramitochondrial Fe/S proteins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the WD repeat CIA1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011492">
        <property type="protein sequence ID" value="KAI7726914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BPK5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000136">
        <property type="entry name" value="Probable cytosolic iron-sulfur protein assembly protein CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03037">
        <property type="entry name" value="ciao1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028608">
        <property type="entry name" value="CIAO1/Cia1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF4">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1 HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="17"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="71"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="115"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="160"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="210"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="314"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7726914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="352" mass="39130" checksum="E0A2F77BB1734D97" modified="2024-05-29" version="1">MPSLDQQTFKLKELQSLKGHTDRVWSLAWNPASGSNGVPAILASCSGDKTVRIWQQRTPSSTSFHCKAVLEDRHDRTVRSCAWSPSGKQLATASFDATTAIWEEIGDEFECVSTLEGHENEVKSVSWNASGSLLATCSRDKCVWIWEVLPGNEFDCISVLTGHTQDVKMVQWHPTVDVLFSCSYDNTIKVWAEDGDSDDWRCVQTLSESNSGHTSTVWALSFNVTGDKMVTCSDDLTIKVWGVDIIRLQSGDVNASWCHLCTMSGYHDRTIFSVHWSREGIIATGAADDAIRLFVESEDHSVDGPSYKLLLKKEKAHTMDINSVQWSPTGNMLLASASDDATIKIWKLESMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BQ66</accession>
    <name>A0AAD5BQ66_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_019340</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011345">
        <property type="protein sequence ID" value="KAI7727591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BQ66"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34360">
        <property type="entry name" value="OS08G0519400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34360:SF1">
        <property type="entry name" value="OS08G0519400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="75"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="100"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7727591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="122" mass="13547" checksum="298666D94C8E062B" modified="2024-05-29" version="1" fragment="single">VAKKFCEPYIDQVAVATKPHLDKARETMKPYTNEAAQVYGKFVGIASKYHQQVQGTVGESLKKHEITRSLATKEFASALLVLAIIILFRALSAIFFGTAKKPTKTSKPSHTRRKAKRALSDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BTL1</accession>
    <name>A0AAD5BTL1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_004326</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011088">
        <property type="protein sequence ID" value="KAI7729059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BTL1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31267">
        <property type="entry name" value="DENTIN SIALOPHOSPHOPROTEIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31267:SF2">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="130"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="233"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="561"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="853"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="960"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="163"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="235"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="286"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="302"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="361"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="374"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="397"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="570"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="627"/>
            <end position="640"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7729059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="1236" mass="135507" checksum="E3F4A1D5E22D093E" modified="2024-05-29" version="1">MPGNGVGDRVHNFFAQENVPQGLRPQDGNWPINDNLWVGNQKQFGVSDSSPRNYSPQQSETERGHGGQHVPDPHIFNSTQTNARPDFARNLSQSHHEQPTSNGYMYGRQNFQTRPDEANFMGVNAEYGQNNVNQRGFPEQPPRNTFRSESSEAHGSLDLFGGQHQTNTQYPGSNSGMKMEHIQQQVGLQNQSVQESPVEMLQDKSVADAASLDPEEEKILFGVQEEWPLEDAAKHQTQTVPSDISLPNMNDKHHGNMGFQDSLRGNRLYESSVPGHRTPEQHGTSQPNNQRNQFNANANAPVPSSVNTGFNMNDTDNSLQQSQNNMKRGNTAGITNLSNLQQGGNHSNQFSPNNHNINHWKHVDSSVKSRGSENSDISQHRLNSKGPQISESSFNSSDKEDLNSSRKENSNDSYRSSSSHLPNTVGQREQFLSDAGRMVSGPGPRKFQHHPMGNLDEDVGAPYGTRQGDNTKSLPLQHARGLGGQDRGSFGLTKTSGQGFGPEVEGALKGSDDMRFKGMIPGHGPNIFAPHDRSVGLSTLDKASQASQSQSQNMLQLLHKVDQSRDGGTARQSNSLERNLSSEIPEPENSDGSFGGHQRSHSSNSQGIGLQLGPPSQRLPMTMQAAKHNSLSQSQPSANSRGKGHAELSPFPPFQEASRGEFKNARMSISGQSQSQTAGHKTMTNHSTVGNESFNGHAPQRHADDKMQVGERLPVSDPRANSGFFQQGAFPKMLPNALSNLQSQQLFSANQAHKVQLNIVESTSLGKQNLEEQEAEKKENKNSLSAQGIITSVGEQSTEKVDQGPMMNGPLAGNDSVNTASQKDLEAFGRSLKPNNFHQQISLLNQMKAYRQTDTDPNSRPMKRLKDSNNLLPGHQVTVPLGNAEIPRPTGPIGNVDRNLASLPANPPPKDALVSHQNSHINPQMAPSWFDQYGTFKGVQMSSQRADAIRTVEQPFKVSGSLETRHDAKEPAASGTTDNSQGGVIWRTPSVPSSSVVDHHFTSPSLKTTGIASQNLVTLRPIKHKWSRDVHSWLKEVSGSSKNHCFNGMVDDDGELIEDVPRPKRRIILSTQLMQQLFPPPPSSLLSTDATLNYESFAFYAARLALGDACNLVSCVSHSESNLLSDKSRESERINDKRLSEAVEGCVSKFTRVENDFSRLDQKASILDLRVECQDLEKFSIINRFAKFHGRGQADGEASSSSSDTVANVPKPFPQRYVVAVPLPKNLPERVHCLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BTM7</accession>
    <name>A0AAD5BTM7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_006066</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleotide-sugar transporter family. CMP-Sialate:CMP antiporter (TC 2.A.7.12) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011277">
        <property type="protein sequence ID" value="KAI7727999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BTM7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015165">
        <property type="term" value="F:pyrimidine nucleotide-sugar transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007271">
        <property type="entry name" value="Nuc_sug_transpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10231">
        <property type="entry name" value="NUCLEOTIDE-SUGAR TRANSMEMBRANE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04142">
        <property type="entry name" value="Nuc_sug_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005799">
        <property type="entry name" value="UDP-gal_transpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="41"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="107"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="151"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="174"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="204"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="245"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7727999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="297" mass="33049" checksum="13496DC9E1A77061" modified="2024-05-29" version="1" fragment="single">MTKDDMIECSVCHSKLVKPTTKTVSRAYDQYRSMTPKKHRALNVLLVVGDCLLVGLQPILVYMSKVDGKFSFSPLSVNFLTEVAKVIFALVMLLIQARNQKPGEKSLLSLSTFVQAARNNVLLAVPALLYAINNYLKFIMQLYFNPATVKMLSNLKVLVIAVLLKIIMKRRFSIIQWEALALLLIGISLNQINSLPDGPSAMGLPVAMGAYIYTFIFVTVPSLASVFNEYALKSQYDTSIYLQNLFLYGYGAIFNFLGILGTVVIQDSSFINITAGANEEILTKDDHFFLPEPIVVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BUQ2</accession>
    <name>A0AAD5BUQ2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ras-related protein RABA6a</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_019989</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Endomembrane system</location>
            <topology evidence="7">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010852">
        <property type="protein sequence ID" value="KAI7730038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BUQ2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01868">
        <property type="entry name" value="Rab11_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000274">
        <property type="entry name" value="ras-related protein RABA5a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0636">Prenylation</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7730038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="218" mass="24874" checksum="E2009820EEDC1E66" modified="2024-05-29" version="1">MGSNLFDHDECDYLFKTILVGDSAVGKSNLLSRFSKDEFHLDSKPTIGVEFAYRNMKIGDKIVKAQVWDTAGQERFRAITSSYYRGALGAMLVYDITRRGTFENLRKWLHELREYGSRDMVIILVGNKSDLIDSREVDTEDGQSLAQLEDLCFMETSAKENLNVEDAFLQMITKIFEIASQKSLEAKEKEATKVLHERKEIIYVVDEVSATKQTSCCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BVL0</accession>
    <name>A0AAD5BVL0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Acyl-CoA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_020015</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010852">
        <property type="protein sequence ID" value="KAI7730064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BVL0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.510">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056819">
        <property type="entry name" value="ACBP4-6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46093">
        <property type="entry name" value="ACYL-COA-BINDING DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46093:SF15">
        <property type="entry name" value="GALACTOSE OXIDASE_KELCH, BETA-PROPELLER, KELCH-TYPE BETA PROPELLER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24922">
        <property type="entry name" value="ACBP4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90257">
        <property type="entry name" value="Myosin rod fragments"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Acyl-CoA-binding" evidence="4">
        <location>
            <begin position="346"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="437"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="445"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF24922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7730064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="461" mass="50323" checksum="F9BB874926034BDB" modified="2024-05-29" version="1">MCSDNSNWHSELDYDRWVALPIAGPRPSARYKHGAFIVDEKLYIVGGSRNGRQLSDVQALDLRNLSWSTIKLKTQPQADNVKNGGLADVFPATSGHSMIKWGNKLLLLGGLSKETSDNVTVRFIDLESQVTGVMEATGKIPTMIWNVAETTKIPPAPRCDHTAAVHVDRYFLIYGGCSHSTFFNDLHVLDLETLEWSQPQIQGDLVSPRAGHAGVSIDEKWFIVGGGDSRSGAVETLVLDMSTLVLSVLMSVERKDTLASEGLTLSSALVNGEQFLVAFGGYNGNYNNEVFVMKPKPKDSKQPKIFKSSAAAAAAASVTAAYALAKSESLDLPAIENSKTKVNLSVEIGIIKEEKKALESSIAWVKAEKSTVKAKLDELNETHTDLSKACSELHSVQGQLAAEKSRCADLETQISELQTKLSSMQSIEEKVQALRSQKSALDHNTPVQRQSYSGSWRWMSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BW72</accession>
    <name>A0AAD5BW72_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_021125</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010729">
        <property type="protein sequence ID" value="KAI7730567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BW72"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7730567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="70" mass="8408" checksum="6D9FC9013AC5C8C6" modified="2024-05-29" version="1" fragment="single">FANELLFALSDVGHVSGGRRAEARKSNHIRIRCHFGVKRRRVRRWRPETTVVEKPEGWFRWFDEDEKKSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BYZ2</accession>
    <name>A0AAD5BYZ2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_027231</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010358">
        <property type="protein sequence ID" value="KAI7731910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BYZ2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025886">
        <property type="entry name" value="PP2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32278">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32278:SF15">
        <property type="entry name" value="F-BOX PROTEIN PP2-B13-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14299">
        <property type="entry name" value="PP2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7731910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="343" mass="40008" checksum="3F31C8ACD376575E" modified="2024-05-29" version="1">MFSKGILLQDDKVLFSLGSNRERNEMVSAKRFSYKNPLLRKWRSLPESRFPKVAEMLYISNLNIRIKIRTQFLSPCINYKVHLIFKFCGSRKSKAKRMYVNLKYKMGNESLNAYFATWREDGWMMIELFQFLNHKKDTDFEVLLESFSRCYCGSDSVYIEGIQFQAIDNASHKKRKKDEMLSANEDMPSNVAIQSRFSGAIEFQRQQVLHIKCEIKIQLLSANAGNVCYLVFKLSEKCCGLHGPVIVRDMFHWRSKETGVLYFRYPNPWNVRDTDWLPRQRKDGWMEELCRALLYVESSFVRSKHLGADVTLSKSPAENGKAEGFSIVQASVLTRSWACRQDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C089</accession>
    <name>A0AAD5C089_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein DETOXIFICATION</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_024967</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010099">
        <property type="protein sequence ID" value="KAI7732976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C089"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11206">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7732976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="76" mass="8304" checksum="2CC939DC7DBED6D3" modified="2024-05-29" version="1" fragment="single">VARGCGWQHLAVYINLGAFYLVGMPVALVVTFLLKLYAKGLWIGLITGLSGQTGALLLLVYLKRWGRVELDGDNNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C0I6</accession>
    <name>A0AAD5C0I6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein VACUOLELESS1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_015370</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Required for vacuole biogenesis and vacuole enlargment in dividing and expanding cells. Involved in the docking or fusion of prevacuolar vesicles.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Vacuole membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the VPS16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010124">
        <property type="protein sequence ID" value="KAI7732892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C0I6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030897">
        <property type="term" value="C:HOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042144">
        <property type="term" value="P:vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.780:FF:000001">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 16 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.780">
        <property type="entry name" value="Vps16, C-terminal region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016534">
        <property type="entry name" value="VPS16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006925">
        <property type="entry name" value="Vps16_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038132">
        <property type="entry name" value="Vps16_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006926">
        <property type="entry name" value="Vps16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12811">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING VPS16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12811:SF0">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 16 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04840">
        <property type="entry name" value="Vps16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04841">
        <property type="entry name" value="Vps16_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF007949">
        <property type="entry name" value="VPS16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0926">Vacuole</keyword>
    <feature type="domain" description="Vps16 N-terminal" evidence="4">
        <location>
            <begin position="8"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="domain" description="Vps16 C-terminal" evidence="3">
        <location>
            <begin position="510"/>
            <end position="827"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR007949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7732892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="845" mass="94297" checksum="8193785E0AB521F6" modified="2024-05-29" version="1">MAGVSIAAEWQLLGDKYYRKPEIYTLHWKNIDLSRNKVACAPFGGPIAIIRDDSKIVQLYSESALRKLRIFSSSGHVISETVWRNPGGRLIGLSWTVDQILICVTQDGTVYRYNIHSKLIEPNVSLGVECFENSVVDCVFWGNGVVCINEAFQLFCIPDLGMGMGNPKVSKLAETGLEEFPLCMAVIEPEYTMSGNVEVLVAVGDHVLVVEEDGVQTVGDGLGPLQKMVVSSNGKLLAAFTHDGQLLVMPTDFSSIIFEYACESALAPEQLVWCGMDSVLLYWDDMLLMVGPYGDPVRYLYDDPLILIPECDGARILSNSNMEFLQRVPASTESIFKIGSTEPAALLYDALDHFDRRSAKADENLRLIRSSLPEAVEACVDAAGHEFDPLLQQTLLRAASYGQAFCSQFQRESIQEMSKTLRVLNAVRSPEIGIPLSIHQYKMLTPSVLIGRLVNAHQHLLALRISDYLGLNQEVVIMHWACSKLSLSSAIPDATLLEILLDKLKLSRSISYASVAAHADQIGRRKLAAMLVEHEPLSSKQVPLLLGIGEEDTALTKATESGDTDLVYLVLFHIWQKRPALELFGMIQARPLARDLFICYARCYKHEFLKDFFLSTGQLHEVAFLLWKESWEVAKNPMASRGSPLLGPRVKLIEKAQNLFAETKEHVFESKAAEEHAKLLKMQHELEVSTKQPIFVDSSISDTIRTCIVLGNHRAALKVKTEFKVSEKRWYWLKVFALVTIRDWDALEKFSKEKRPPTGFKPFVEACIDADEKSEAVKYISKLADPREKAEAFARIGMAKEAAEAASQSKDGELLERLKNTLQQNTTASSIFDTLRDRLSFPGVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C0P2</accession>
    <name>A0AAD5C0P2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">M8C21_006165</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="25">AA19_3_7</strain>
            <tissue evidence="25">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="25">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="EMBL" id="JAMZMK010010065">
        <property type="protein sequence ID" value="KAI7733201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C0P2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000150">
        <property type="entry name" value="Putative LRR receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000015">
        <property type="entry name" value="Somatic embryogenesis receptor kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000016">
        <property type="entry name" value="Somatic embryogenesis receptor-like kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051824">
        <property type="entry name" value="LRR_Rcpt-Like_S/T_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48006">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN DDB_G0281931-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48006:SF102">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN DDB_G0281931-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="16 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="12 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-0675">Receptor</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10 23" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 22" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="23">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042214307" description="non-specific serine/threonine protein kinase" evidence="23">
        <location>
            <begin position="23"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="213"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="24">
        <location>
            <begin position="280"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="308"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="KAI7733201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="603" mass="67338" checksum="88A24FDC6F4B00DF" modified="2024-05-29" version="1">MKLVFVLLVLGNLCFVAPDAQGDALFDLRTSLKASPDQLTDWNQNQVNPCTWSKVTCDPNNKVVSVSMSFMGFTGSLSPRIGVLSKLATLSLQGNQITGGIPEDYGNLTSLTMLDLENNRLTGPIPSSLGNLINLQLLILSQNNLEGPIPDSLSSLPNLTSILLGSNNLSGQIPDHLFQVSKFNFTGNRLNCGFNNTRPCTSPSGDASKKSKAGILIGVIAGVLVLILLGCLMLFFWRGRHKGYKREVFVDVPGEVDRRIAFGQLKRFAWRELQVATDNFSERNVLGQGGFGKVYKGVLGDNTKVAVKRLTDYESPGGDAAFQREVEMISVAVHKNLLRLIGFCTTPTERLLVYPYMHNLSVAYRLRELKPGEAVLDWATRKRVALGTARGLEYLHEHCNPKIIHRDVKAANVLLDEDFEAVVGDFGLAKLVDVRKTNVTTQVRGTMGHIAPEYLSTGKSSERTDIFGYGIMLLEIVTGQRAIDFSRLEEEDDVLLLDHVKKLEREKRLDAIVDRNLNKRYNMEEVEMMIKVALLCTQGSPEDRPAMSEVVRMLEGEGLAERWEEWQNVEVTRRQEYDRLQRRFDWGEDSIYNQEAIELSGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C119</accession>
    <name>A0AAD5C119_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_004271</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010101">
        <property type="protein sequence ID" value="KAI7732975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C119"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11076">
        <property type="entry name" value="CYP78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000016">
        <property type="entry name" value="Cytochrome P450 78A5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051996">
        <property type="entry name" value="Cytochrome_P450_78A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47946:SF1">
        <property type="entry name" value="CYTOCHROME P450 78A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47946">
        <property type="entry name" value="CYTOCHROME P450 78A7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8 9" id="KW-0349">Heme</keyword>
    <keyword evidence="6 8 9" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="25"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="8">
        <location>
            <position position="468"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7732975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="532" mass="59905" checksum="3A4D409F49014931" modified="2024-05-29" version="1">MTTHIDSLWLFALVSKSKCTTSINFYLFVTLFALACCIKVLYQWARPGGPAWSKTKVKSTIPGPKGYPFIGSMYLMTGLAHRKIAAAANYYGAGRLMAFSMGETRVVVTCNPEVAKDVLNSSAFADRPIKESAYGLMFNRAIGFAPYGVYWRTLRRIAATHLFCPKQIKVSEHQRTVISSQMVEMLQNQQRTNSSVVVREVLKRASLSNMMWSVFGRKYRVDSNDFDSVELRKLVDEGYNLLGVLNWTDHLPWLSDIDLQGVRSRCSKLVPQVNRFVKRIIDEHRAQPSHLNSDFTDVLLSLEGPDKLSEPDMIAVLWEMIFRGTDTVAVLIEWILARLVLHADVQQKVQDELDRVVGRSRAVTEADITSLMYLPAVVKEVLRLHPPGPLLSWARLAIIDTVVDGHQVPAGTTAMVNMWAITRDPQIWNEPLEFRPERFLNETEGLMDMSVMGSDLRLAPFGSGRRSCPGKSLGLATVSFWVASLLQEFEFGPCDGYDVDLSEVLKLSCEMANPLRVTLRSRPRPTVLNREV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C183</accession>
    <name>A0AAD5C183_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_006112</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010280">
        <property type="protein sequence ID" value="KAI7732229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C183"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03261">
        <property type="entry name" value="ABC_Org_Solvent_Resistant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43023">
        <property type="entry name" value="PROTEIN TRIGALACTOSYLDIACYLGLYCEROL 3, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43023:SF3">
        <property type="entry name" value="PROTEIN TRIGALACTOSYLDIACYLGLYCEROL 3, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="59"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7732229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="293" mass="31863" checksum="81F42833B040EE2A" modified="2024-05-29" version="1">MNSFVGSSVSALNWLNVSHPSSGVAPHNLKPADEFSSSKFQDSSVNLYSKGSEATDVLIECKDVYKSFGEKHILRGVNFKIKHGEAVGIIGPSGTGKSTVLKIIAGLLTPDKVFQSAALFDSLTVRENVGFLLYEHSRMPEDKIKELVAETLAAVGLKEVEDRMPSELSGGMKKRVALARSIIYDTKNNTVEPEVLLYDEPTAGLDPIASTVVEDLIRSVHCKGEDAVGKPVKIASYVVVTHQHSTIRRAVDRLVFLHEGKVVWEGTTHEFTSSTNPIVQQFAAGNLDGPIKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C2U0</accession>
    <name>A0AAD5C2U0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Mechanosensitive ion channel protein 2, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_033426</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MscS (TC 1.A.23) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010094">
        <property type="protein sequence ID" value="KAI7733069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C2U0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034220">
        <property type="term" value="P:monoatomic ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023408">
        <property type="entry name" value="MscS_beta-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006685">
        <property type="entry name" value="MscS_channel_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056876">
        <property type="entry name" value="Msl2-3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057483">
        <property type="entry name" value="MSL2/3_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045042">
        <property type="entry name" value="YnaI-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43634:SF2">
        <property type="entry name" value="LOW CONDUCTANCE MECHANOSENSITIVE CHANNEL YNAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43634">
        <property type="entry name" value="OW CONDUCTANCE MECHANOSENSITIVE CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00924">
        <property type="entry name" value="MS_channel_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24956">
        <property type="entry name" value="Msl2-3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25237">
        <property type="entry name" value="MSL2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0407">Ion channel</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="113"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="196"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="237"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="268"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="Mechanosensitive channel protein 2/3 transmembrane" evidence="12">
        <location>
            <begin position="156"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="Mechanosensitive ion channel MscS" evidence="10">
        <location>
            <begin position="286"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="Mechanosensitive ion channel protein 2/3 C-terminal" evidence="11">
        <location>
            <begin position="361"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="479"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="504"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="528"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF24956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF25237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7733069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="597" mass="66520" checksum="E49FC74DC4EF1010" modified="2024-05-29" version="1" fragment="single">MTMAGSLNFSYKWGIHKNQRYNKQFETAMYNRQGMVGSDKLHMLPAGLSSLASQQDLFGVKISVGLQRPLSAASNRFNLVKCNCSLNPVQAFSASAVKNAALALSSGLQGKPIILKLLPAVGIVVFAVWGLGPLMRRTRTIFLHKSDGSWGKSNSHYVMTSYLQPLLLWTGAILICRALDPIVLSSEASQIVKNRLLTFVKSLSTVLAFAYCLSSAIQQTQNFFMQTSDATDTRNMGFQFAGKAVYTAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSIMIHATRPFVVNEWIKAKIEGYEVSGTVEHVGWWSPTIIRGEDREAIHIPNHKFTMNVVRNMTQKTHWRIKTHLAISHLDVNKINATDMRKVLAKNPQVEQQRLHRRVFLENVDPENQSLLILVSCFVKTPHHEEYLCVKEAILLDLLRVISHHRARLATPIRTVQKMYNDSDMDSYPFAESVFTRGRAPPHRPLLLMEPNYKVNDEKSKGQNSKSSTKSNNKTEGKSASASSDSKTKETPTPDVKSIDKKSNSDAKDKKPNSETTIRDKNVDDGKPSNEAVGNDSVTEQKAPRPAPMEENIVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C2U9</accession>
    <name>A0AAD5C2U9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">M8C21_016855</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">AA19_3_7</strain>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Probable ATP-dependent zinc metallopeptidase. Involved in the assembly and/or stability of the complexes I and V of the mitochondrial oxidative phosphorylation system.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
            <orientation evidence="2">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the peptidase M41 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the N-terminal section; belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010018">
        <property type="protein sequence ID" value="KAI7733358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C2U9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005745">
        <property type="term" value="C:m-AAA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034982">
        <property type="term" value="P:mitochondrial protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19501">
        <property type="entry name" value="RecA-like_FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000019">
        <property type="entry name" value="AFG3-like AAA ATPase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000001">
        <property type="entry name" value="ATP-dependent zinc metalloprotease FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1690.20:FF:000004">
        <property type="entry name" value="ATP-dependent zinc metalloprotease FTSH 10 mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.760:FF:000005">
        <property type="entry name" value="ATP-dependent zinc metalloprotease FTSH 10, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1690.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.760">
        <property type="entry name" value="Peptidase M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01458">
        <property type="entry name" value="FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050928">
        <property type="entry name" value="ATP-dep_Zn_Metalloprotease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005936">
        <property type="entry name" value="FtsH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011546">
        <property type="entry name" value="Pept_M41_FtsH_extracell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000642">
        <property type="entry name" value="Peptidase_M41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037219">
        <property type="entry name" value="Peptidase_M41-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01241">
        <property type="entry name" value="FtsH_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43655:SF2">
        <property type="entry name" value="AFG3 LIKE MATRIX AAA PEPTIDASE SUBUNIT 2, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43655">
        <property type="entry name" value="ATP-DEPENDENT PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06480">
        <property type="entry name" value="FtsH_ext"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01434">
        <property type="entry name" value="Peptidase_M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140990">
        <property type="entry name" value="FtsH protease domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="17" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042181396" description="AAA+ ATPase domain-containing protein" evidence="17">
        <location>
            <begin position="16"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="18">
        <location>
            <begin position="215"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="643"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="647"/>
            <end position="661"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00057665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAI7733358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="669" mass="73589" checksum="8ADA2BF48A4CAEBC" modified="2024-05-29" version="1">MLVIGLLLFSSLSFGPREQKQISFQEFKNKLLEPGLVDHIVISNKSVAKVYVRNTPRNQSTDGAMEGIDSPSKVNTSQYKYYFNIGSVESFEEKLEEAQEALGIDPHDYVPVTYASEMVWYQEIMRFAPTLLLLGSLWYMSRRMQGGLGVGGGGGKGARGIFNIGKAHINKVDKNAKNKVFFNDVAGCDEAKQEIMEFVHFLKNPKKYEELGAKIPKGALLVGPPGTGKTLLAKATAGESGVPFLSISGSDFMEMFVGVGPSRVRNLFQEARQCAPSIIFIDEIDAIGRARGRGGFSGSNDERESTLNQLLVEMDGFGTTAGVVVLAGTNRPDILDKALLRPGRFDRQISIDKPDIKGREQIFQIYLKKLKLDNEPSYFSQRLAALTPGFAGADIANVCNEAALIAARGEKTKITMEHFEGAIDRVIGGLEKKNKVISKLERRTVAYHEAGHAVVGWFLEHAEPLLKVTIVPRGTAALGFAQYVPSENLLMTKEQLFDMTCMTLGGRAAEQVILGKISTGAQNDLEKVTKMTYAQVAVYGFSEKVGLLSFPQREDGMEMTKPYSSKTGAIIDTEVREWVNKAYTRTVSLVEEHKEKLSQIAELLLKKEVLHQEDLLKVLGERPFKAAEMTNYDIFKLGFEDEAKTGQAEQGSGSGEDSQTPLEPDVVPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C8B2</accession>
    <name>A0AAD5C8B2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Auxin-responsive protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_016091</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Aux/IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimers and heterodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Aux/IAA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009276">
        <property type="protein sequence ID" value="KAI7736418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C8B2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009734">
        <property type="term" value="P:auxin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033389">
        <property type="entry name" value="AUX/IAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003311">
        <property type="entry name" value="AUX_IAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053793">
        <property type="entry name" value="PB1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31734">
        <property type="entry name" value="AUXIN-RESPONSIVE PROTEIN IAA17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31734:SF87">
        <property type="entry name" value="AUXIN-RESPONSIVE PROTEIN IAA5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02309">
        <property type="entry name" value="AUX_IAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51745">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0927">Auxin signaling pathway</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0678">Repressor</keyword>
    <keyword evidence="5 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 8" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="PB1" evidence="9">
        <location>
            <begin position="60"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7736418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="149" mass="16804" checksum="8BEA76881A11EE45" modified="2024-05-29" version="1">MGTQVLGLEITELRLGLPGGCDDGDGGDKRGKKREVDMVVGWPPVCSYRKKSMRDDVATKVYVKVSMDGVPILRKVDLGCFKGYWELGIALEKLFDCYGIGEAMKEDNGSCEYTTIYEDKDGDWMLVGDVPWMMFIQTCKRLRVKRRSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CAU8</accession>
    <name>A0AAD5CAU8_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_015667</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008813">
        <property type="protein sequence ID" value="KAI7738437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CAU8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7738437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="46" mass="5323" checksum="DA63A0817CADD04A" modified="2024-05-29" version="1">MLVFYSSELCASSIGVAFRFPWLLLDKEWPIAMNVHDFLFSQLQGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CBD5</accession>
    <name>A0AAD5CBD5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Hydroxymethylglutaryl-CoA synthase</fullName>
            <shortName evidence="5">HMG-CoA synthase</shortName>
            <ecNumber evidence="5">2.3.3.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">3-hydroxy-3-methylglutaryl coenzyme A synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_018054</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the condensation of acetyl-CoA with acetoacetyl-CoA to form HMG-CoA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>acetoacetyl-CoA + acetyl-CoA + H2O = (3S)-3-hydroxy-3-methylglutaryl-CoA + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10188"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43074"/>
            <dbReference type="ChEBI" id="CHEBI:57286"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.3.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the thiolase-like superfamily. HMG-CoA synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.10" evidence="5"/>
    <dbReference type="EMBL" id="JAMZMK010008880">
        <property type="protein sequence ID" value="KAI7738065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CBD5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004421">
        <property type="term" value="F:hydroxymethylglutaryl-CoA synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006084">
        <property type="term" value="P:acetyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010142">
        <property type="term" value="P:farnesyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016126">
        <property type="term" value="P:sterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00827">
        <property type="entry name" value="init_cond_enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000008">
        <property type="entry name" value="3-hydroxy-3-methylglutaryl coenzyme A synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000590">
        <property type="entry name" value="HMG_CoA_synt_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013746">
        <property type="entry name" value="HMG_CoA_synt_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013528">
        <property type="entry name" value="HMG_CoA_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010122">
        <property type="entry name" value="HMG_CoA_synthase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01833">
        <property type="entry name" value="HMG-CoA-S_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43323">
        <property type="entry name" value="3-HYDROXY-3-METHYLGLUTARYL COENZYME A SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43323:SF15">
        <property type="entry name" value="HYDROXYMETHYLGLUTARYL-COA SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08540">
        <property type="entry name" value="HMG_CoA_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01154">
        <property type="entry name" value="HMG_CoA_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01226">
        <property type="entry name" value="HMG_COA_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="5" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="5" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Hydroxymethylglutaryl-coenzyme A synthase N-terminal" evidence="6">
        <location>
            <begin position="3"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="Hydroxymethylglutaryl-coenzyme A synthase C-terminal" evidence="7">
        <location>
            <begin position="176"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="3">
        <location>
            <position position="84"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="3">
        <location>
            <position position="118"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="3">
        <location>
            <position position="248"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="253"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR610122-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR610122-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7738065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="458" mass="50673" checksum="41DCFA2AF08B6589" modified="2024-05-29" version="1">MAPQNVGILAMEIYFPPTCIQQDVLEDFDGASKGKYTIGLGQDCMAFCSEVEDVISMGLTAVTSLLEKYEIDPKQIGRMEVGSETVIDKSKSIKTFLMKIFEDCGNTDIEGVDSTNACYGGTAALFNCVNWVESSSWDGRYGLVVSTDSAVYAEGPARPTGGAGAVAMLIGPDAPIAFESKFRASHMSHAYDFYKPDLASEYPVVDGKLSQTCYLMALDSCYTAYCQKYEKLQGKQFSMADADYFVFHSPYNKLVQKSFARLVFDDVARNASSVDESAKEKLGQFTSLKGDESYQSRDLEKASQQVAKPEYDRKVQPGTLVPKQLGNMYTASLYAAFASLIHNKTSSLDGKRVMMFSYGSGLTATMFSLHLTEGKAPFSLSNIAKVMNIEHKLKIRTELAPEKFVELMHVMEHRYGGKDYETSKDTSHLAPGTYYLTHVDSKYRRYYAKKDTAVANGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CCY7</accession>
    <name>A0AAD5CCY7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Zinc finger LSD1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_022632</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008603">
        <property type="protein sequence ID" value="KAI7739607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CCY7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040319">
        <property type="entry name" value="LSD1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005735">
        <property type="entry name" value="Znf_LSD1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01053">
        <property type="entry name" value="LSD1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31747:SF1">
        <property type="entry name" value="PROTEIN LOL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31747">
        <property type="entry name" value="PROTEIN LSD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06943">
        <property type="entry name" value="zf-LSD1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zinc finger LSD1-type" evidence="3">
        <location>
            <begin position="28"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="Zinc finger LSD1-type" evidence="3">
        <location>
            <begin position="67"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="Zinc finger LSD1-type" evidence="3">
        <location>
            <begin position="105"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF06943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7739607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="145" mass="15092" checksum="D4981889BDF4592F" modified="2024-05-29" version="1">MPVPLAPYPSPPVPLTSPENGSQSQLVCSGCKNLLLYPVGATSVCCAVCNAVTAVPPPGTEMAQLVCGGCHTLLMYIRGATSVQCSCCHTINLALEANQVAHVNCGSCRMLLMYQYGARCVKCAVCQFVTSIGASTSATEQKFNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CFH2</accession>
    <name>A0AAD5CFH2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_009428</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008454">
        <property type="protein sequence ID" value="KAI7740355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CFH2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051992">
        <property type="entry name" value="OxStress_Response_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33172">
        <property type="entry name" value="OS08G0516900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33172:SF96">
        <property type="entry name" value="PROTEIN OXIDATIVE STRESS 3 LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="37"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="166"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="49"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="61"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="77"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="167"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7740355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="228" mass="24666" checksum="6E6E87AC6C677ECE" modified="2024-05-29" version="1">MSVAFETNSNSGSNTTNRIQLPHAMWICNSTGNPWSTSLPAVKEEDDSSASSSIGNNSDADCSDDDDDHDGEVQSKDNNNNNNGVINGLNDLEKVLPIKRGISTFYAGKSKSYGSLADAVSAPSIQDIVKPEDAYSRKRKNMLAHHVLLDKNRELSCENGISKRLARNSNESASQSRCLPPLPRSSRKVSSNESASRMYMSAPWRSLSLSDLQHASGLSNKGNEVDGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CFH9</accession>
    <name>A0AAD5CFH9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">RNA-binding protein NOB1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_028018</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the NOB1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008533">
        <property type="protein sequence ID" value="KAI7740000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CFH9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031981">
        <property type="term" value="C:nuclear lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09876">
        <property type="entry name" value="PIN_Nob1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000020">
        <property type="entry name" value="20S-pre-rRNA D-site endonuclease NOB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.210.10">
        <property type="entry name" value="Nin one binding (NOB1), Zn-ribbon-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039907">
        <property type="entry name" value="NOB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017117">
        <property type="entry name" value="Nob1_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036283">
        <property type="entry name" value="NOB1_Zf-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014881">
        <property type="entry name" value="NOB1_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033411">
        <property type="entry name" value="Ribonuclease_PIN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12814">
        <property type="entry name" value="RNA-BINDING PROTEIN NOB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12814:SF2">
        <property type="entry name" value="RNA-BINDING PROTEIN NOB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17146">
        <property type="entry name" value="PIN_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08772">
        <property type="entry name" value="Zn_ribbon_NOB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037125">
        <property type="entry name" value="D-site_20S_pre-rRNA_nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144206">
        <property type="entry name" value="NOB1 zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8 9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Ribonuclease PIN" evidence="13">
        <location>
            <begin position="65"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="Nin one binding (NOB1) Zn-ribbon-like" evidence="12">
        <location>
            <begin position="449"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="218"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="299"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="362"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="32"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="308"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="459"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="462"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="474"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="477"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR037125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR037125-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF08772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF17146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7740000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="597" mass="65619" checksum="76A4D5C646B6B7E2" modified="2024-05-29" version="1">MEALPKPMEPPPNPTPCWSNIVKQQPQPPQNPNNNNNNNNNNPSQSMATALVGSCKSTKGIAVAVVDANAIIQGGEQLHHAADKFVSVSDVIQEIRDPMSRHRLNVLPFTVETMEPSPDSLKKVISFARATGDLQTLSDVDLKLIALTYTLEAQIHGTSHLRDSPPPIRTVNVKRLPEKELPGWGSNVPNLAQWEALEHAAGDGSGPESRILPLKDLNMNGIVPGDKDQDNEDSASANGSEVHDNGASGKPRKYLPKKKEVVIEGKKMVAAGIDASQGQVDGDNADDWLPAVSRSTHRRFLRRKARRERYESSEKDVQDDGNEESVDDTKVPDLIYENSDELCGGEKTNDASNGGEDISTALDQMRLQEDGEENEIENVSEELEHLDMTSETHESVDASSYIDDASSEQSWMLRSLSESSVACVTGDFAMQNVILQMGLRLVAPGGMQIRELHRWILKCHACFKVTMEIGRIFCPSCGNGGTLRKVAVTVGENGAIIAARRPRVSLRGTKFSLPLPQGGRDAITKNPILREDQLPQKFLHPKTKKKNKEADDIFATNDIFIHHSDKRAPLQPPVRKALAVFSGKRNPNDNHYSRAKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CFW5</accession>
    <name>A0AAD5CFW5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_006499</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NRAMP (TC 2.A.55) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008329">
        <property type="protein sequence ID" value="KAI7740857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CFW5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015086">
        <property type="term" value="F:cadmium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005384">
        <property type="term" value="F:manganese ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034755">
        <property type="term" value="P:iron ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00221">
        <property type="entry name" value="NRAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001046">
        <property type="entry name" value="NRAMP_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01197">
        <property type="entry name" value="nramp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF037982">
        <property type="entry name" value="Nramp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001923">
        <property type="entry name" value="PRK00701.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11706:SF77">
        <property type="entry name" value="METAL TRANSPORTER NRAMP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11706">
        <property type="entry name" value="SOLUTE CARRIER PROTEIN FAMILY 11 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01566">
        <property type="entry name" value="Nramp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00447">
        <property type="entry name" value="NATRESASSCMP"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="47"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="85"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="128"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="158"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="187"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="227"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="268"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="332"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="372"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="397"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="437"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="470"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="525"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="30"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7740857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="546" mass="59250" checksum="E31561BC0365E3A8" modified="2024-05-29" version="1">MAGLQHEPRVTGGSNRISAVDLGDNTPVHNSKDIDHKYTNNDQKPGWKKFLSFVGPGFLVSLAYLDPGNMETDLQAGANHRYELLWIILIGLIFALVIQSLAANLGVSTGKHLSELCKAEYPIVVKYCLWLLAELAVIAADIPEVIGTAFGLNILFKIPVWVGVLLTGLSTLLLLALQKYGVRKLEILIAILVFVMAACFFGEMSYVKPPAGEVLKGMFVPKLSGQGATGDAIALLGALVMPHNLFLHSALVLSRKIPNSVRGINDACRYFLIESGIALFVAFLINVSMISVSGTVCSAKNLSSDDMDRCSDLNLNSASFLLKNVLGKSSSTLYGIALLASGQSSTITGTYAGQFIMQGFLDLKMRKWLRNLMTRCIAITPSLIVSIIGGSAGAGRLIVIASMILSFELPFALIPLLKFSSSATKMGPHKNSMIIIVFSWVLGLCIIGINIYYLITAFVGWLIHSSLPKVANVFIGIIVFPAMAIYILSVIYLMFRKDVVVTFIEPEKVDPNAEINMENGQYSVDKRPGLTNAPYREDLADIPLPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CGB8</accession>
    <name>A0AAD5CGB8_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_001134</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the phosphatidylethanolamine-binding protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008250">
        <property type="protein sequence ID" value="KAI7741188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CGB8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009908">
        <property type="term" value="P:flower development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010228">
        <property type="term" value="P:vegetative to reproductive phase transition of meristem"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00866">
        <property type="entry name" value="PEBP_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.280.10:FF:000001">
        <property type="entry name" value="Terminal flower 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.280.10">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008914">
        <property type="entry name" value="PEBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036610">
        <property type="entry name" value="PEBP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035810">
        <property type="entry name" value="PEBP_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001858">
        <property type="entry name" value="Phosphatidylethanolamine-bd_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11362">
        <property type="entry name" value="PHOSPHATIDYLETHANOLAMINE-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11362:SF129">
        <property type="entry name" value="PHOSPHATIDYLETHANOLAMINE-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01161">
        <property type="entry name" value="PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49777">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01220">
        <property type="entry name" value="PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7741188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="168" mass="18997" checksum="01486005FBEC4FD0" modified="2024-05-29" version="1">MSLVVGRVIGDVVDQFTPSVQMDVTYNPHCPVANGHELKPNLVASKPRVHIGGVDMRSSYTLVMTDPDAPSPSDPYLREHLHWIVTDIPGTTDATFGREIVSYEKPKPVIGFHRYVFLLFKQRARQSVRPPVSRDRFNTRAFAQENDLGLPVAAIYFNAQRENAPRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CHK0</accession>
    <name>A0AAD5CHK0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Myosin N-terminal SH3-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_013894</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008385">
        <property type="protein sequence ID" value="KAI7740646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CHK0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003774">
        <property type="term" value="F:cytoskeletal motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004009">
        <property type="entry name" value="SH3_Myosin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02736">
        <property type="entry name" value="Myosin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51844">
        <property type="entry name" value="SH3_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="5" id="KW-0505">Motor protein</keyword>
    <keyword evidence="4 6" id="KW-0518">Myosin</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myosin N-terminal SH3-like" evidence="8">
        <location>
            <begin position="9"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="Myosin motor" evidence="7">
        <location>
            <begin position="63"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7740646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="93" mass="10267" checksum="6AB6C6C61777C1C9" modified="2024-05-29" version="1" fragment="single">MKAAATNIIVGSQVWVEDPEVAWIDGDVLEINAANIKVKLTSGKEIVTTSAHAYPKDPEFPSCGVDDMTKLAYLHEPGVLENLKSRYNMDEIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CIC2</accession>
    <name>A0AAD5CIC2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Transcription factor DIVARICATA-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_006468</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008277">
        <property type="protein sequence ID" value="KAI7741066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CIC2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000009">
        <property type="entry name" value="transcription factor MYB1R1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006447">
        <property type="entry name" value="Myb_dom_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01557">
        <property type="entry name" value="myb_SHAQKYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44042">
        <property type="entry name" value="DUPLICATED HOMEODOMAIN-LIKE SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44042:SF39">
        <property type="entry name" value="MYB DOMAIN, PLANT, HOMEODOMAIN-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="SANT" evidence="7">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="8">
        <location>
            <begin position="68"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="6">
        <location>
            <begin position="69"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="7">
        <location>
            <begin position="77"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7741066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="179" mass="20634" checksum="867812299162063A" modified="2024-05-29" version="1" fragment="single">RWSNVAAFVPGKSESEVREQYEALKVDINNIEAGLVEDPGYLSVTSEFLQDHGLKSFANRLYRSRSFDPERRKGIPWTEDEHRRFLMGLQVHGKGDWRNISRNFVVTKTPTQVASHAQKYYARQHSEGKEKRRPSIHDITTVHLDKSTPLVNTPNNEMINIRAYNSGLYFQPTGYQVQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CIU4</accession>
    <name>A0AAD5CIU4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">30S ribosomal protein S13, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_026178</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008005">
        <property type="protein sequence ID" value="KAI7742379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CIU4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.50:FF:000001">
        <property type="entry name" value="30S ribosomal protein S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.910.10">
        <property type="entry name" value="30s ribosomal protein s13, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01315">
        <property type="entry name" value="Ribosomal_uS13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027437">
        <property type="entry name" value="Rbsml_uS13_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001892">
        <property type="entry name" value="Ribosomal_uS13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010979">
        <property type="entry name" value="Ribosomal_uS13-like_H2TH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019980">
        <property type="entry name" value="Ribosomal_uS13_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018269">
        <property type="entry name" value="Ribosomal_uS13_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03631">
        <property type="entry name" value="uS13_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10871">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S13/40S RIBOSOMAL PROTEIN S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10871:SF1">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US13M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00416">
        <property type="entry name" value="Ribosomal_S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46946">
        <property type="entry name" value="S13-like H2TH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00646">
        <property type="entry name" value="RIBOSOMAL_S13_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50159">
        <property type="entry name" value="RIBOSOMAL_S13_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7742379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="171" mass="19061" checksum="C967C68ECB9C4406" modified="2024-05-29" version="1">MAQSLATPVAPSLSLISTGLPLFNSKPTSNSLSFSTFSPPKVGGLSIRCARVGGVEIPNNKRVQYSLQYIHGIGRTRALEILNDLKMENKITKDLTEDELIVLRDEVSKYMIEGDLRRFNALAIRRLKEIQCYRGIRHIQGLPCRGQRTKNNCRTLKGKKVAIAGKKKAPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CJ66</accession>
    <name>A0AAD5CJ66_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FCP1 homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_022338</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008179">
        <property type="protein sequence ID" value="KAI7741531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CJ66"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07521">
        <property type="entry name" value="HAD_FCP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000112">
        <property type="entry name" value="CTD small phosphatase-like protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011948">
        <property type="entry name" value="Dullard_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050365">
        <property type="entry name" value="TIM50"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02251">
        <property type="entry name" value="HIF-SF_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12210">
        <property type="entry name" value="DULLARD PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FCP1 homology" evidence="1">
        <location>
            <begin position="87"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7741531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="299" mass="33823" checksum="9B040330DF4B6D55" modified="2024-05-29" version="1">MAEIGQATSSEVYGPTTTFQVWRTLLNWLTFFFQIFVQIVRGTPSVLINYSSSSTTSFKPLPVVESSEPSNLADASVNIPSVVADYDRLEKLTVVLDLDETLVCAYETSGLPDSIRKQATEAGLTWFELECVSSDKEGDGKPKVNYVTVFERPGLHEFLNQLSKFAELVLFTAGLEGYAKPLVDRIDAENRISRRFYRPSTISTEYREHVKDLSFVSKDFCRIVIVDNNPFSFLLQPVNGIPCIPFSAGQPHDDQLLEVLLPLLKQLSEQRDVRPVLYERFHMPEWFQKHGIPASGFTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CJV9</accession>
    <name>A0AAD5CJV9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">SUN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_009341</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007788">
        <property type="protein sequence ID" value="KAI7743168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CJV9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043495">
        <property type="term" value="F:protein-membrane adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045119">
        <property type="entry name" value="SUN1-5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012919">
        <property type="entry name" value="SUN_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12911:SF8">
        <property type="entry name" value="KLAROID PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12911">
        <property type="entry name" value="SAD1/UNC-84-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07738">
        <property type="entry name" value="Sad1_UNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51469">
        <property type="entry name" value="SUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="84"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="SUN" evidence="7">
        <location>
            <begin position="239"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="26"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="55"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="67"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7743168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="417" mass="46374" checksum="249160CD211CA70C" modified="2024-05-29" version="1">MSSSTVSRSNASLRSRPTTDKKPTQDFPTTTDDISHSPLTTDPTHPTKPIEPDTSKQPQIPTSSTTKLRKRTLVKKPTRPPWKTAASVLVKNLGVLLILLLLFQMVRRLGFNQAGIVSGVFDSDYEKRIAEVEAFLKTTTKMMQVQVDVVDRKIESEIKGLKTELSKRIDDEKVAVGQKFGEFDRRVGSVEKMLSGSEWLSKEEFDRFVEEFGKGGGEVKLDEIRGLAKEIVEREIGKHAADGLGRVDYAVATGGAKVLRHSEAFSASRVMGWFSGNVRSDAVKVLQPSFGQPGECFPLKGDNGFVDVKLRTAIVPEAVTLEHVSKSVAFDISSAPKHCKVWGWLQNDEDTQKEHLLTEFTYDLEKSIAQTFNVLDTKGEDQIVIDTIRLELLSNHGSPTHTCIYRVRVHGHQPNDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CLS0</accession>
    <name>A0AAD5CLS0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_031064</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007551">
        <property type="protein sequence ID" value="KAI7744286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CLS0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010200">
        <property type="term" value="P:response to chitin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26374:SF413">
        <property type="entry name" value="ZINC FINGER C2H2-TYPE_INTEGRASE DNA-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26374">
        <property type="entry name" value="ZINC FINGER PROTEIN ZAT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="35"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="73"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7744286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="149" mass="16679" checksum="B5BE422E7FAB162D" modified="2024-05-29" version="1" fragment="single">RNWEDDREVENLAMANCLMLLNRVGGSGSCSQRVFRCKTCNKQFSSFQALGGHRTSHKKVKPVDESPTKAKTHECSICGLEFDRGQALGGHMRRHRDSQLEKNVPAKATAEEIDGRRGRGLCLDLDLNLTPFMNELKIWSRSTGTTTTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-10-08" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CLS8</accession>
    <name>A0AAD5CLS8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Late embryogenesis abundant protein LEA-2 subgroup domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_028887</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007536">
        <property type="protein sequence ID" value="KAI7744438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098542">
        <property type="term" value="P:defense response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044839">
        <property type="entry name" value="NDR1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31234">
        <property type="entry name" value="LATE EMBRYOGENESIS ABUNDANT (LEA) HYDROXYPROLINE-RICH GLYCOPROTEIN FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31234:SF2">
        <property type="entry name" value="OS05G0199100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="113"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="9"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7744438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="303" mass="33664" checksum="E8B4266A0C08E402" modified="2024-05-29" version="1">MQHTKTDSDATSLTASSPPRSPPRRPVYYVQSPSHXXXXTTLSYNSTPAASPPHYAHHSRESSATRFSGKVSSSNDVALKSFAKPWDHTQFNSIEEEALLNDDESEKRIPRRCYVFGFMLCFLVLFFVFAGILYGASKPQKPKLTLRSITFERFVVQAGSDFTGVATDMVSMNSTVKFMFRNTATFFGLHVTSSPLELSFSQLAVGSGNMKKFYQSRKSQRLVTVSVIGDKIPLYGSGQALKSSKGIPTSPVPLKLSFVVRSRGYVLGKLVKPKFFNRVECSVAFDPQKINVPMALKNHCKFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CMA1</accession>
    <name>A0AAD5CMA1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FBD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_013544</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007561">
        <property type="protein sequence ID" value="KAI7744247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CMA1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006566">
        <property type="entry name" value="FBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050232">
        <property type="entry name" value="FBL13/AtMIF1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055411">
        <property type="entry name" value="LRR_FXL15/At3g58940/PEG3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31900">
        <property type="entry name" value="F-BOX/RNI SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31900:SF31">
        <property type="entry name" value="F-BOX_LRR-REPEAT PROTEIN 13-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08387">
        <property type="entry name" value="FBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24758">
        <property type="entry name" value="LRR_At5g56370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00579">
        <property type="entry name" value="FBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FBD" evidence="1">
        <location>
            <begin position="252"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7744247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="357" mass="40521" checksum="532A38AB742A0004" modified="2024-05-29" version="1" fragment="single">DKAVRLNVRELDIQVNVLELPLSFFTCKTIKKLRLDHISYDHDVWESVHLPCLKTLDIAIYTNPLVNVFKLVSGCPILESLSLEVQCCQYEEDYLFDIPTLKRLKLTFYCSQSFSNTVILNVPNLEYLFIGGNLRSLFVMEDVSSLVEATVSFTNLSIGYLWAELLKGLNGVNSLSAQADGFPYLVNTFCSPLPIFLNMRCLELKGFCESGLISQFLEGSPALKHLCIEKVYELLLQEPGYTSWIEPKSVPQCMLTNLTSIKFLNCKGHKCDIQFLVYALGNAKVLKKVTVIWESLSLEKRMQLSAQLLKLPRASQDCEICFLGETAYIDLRLGNEEWTVETRIVIEESKIPLRAYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CNB3</accession>
    <name>A0AAD5CNB3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_009728</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007709">
        <property type="protein sequence ID" value="KAI7743631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CNB3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7743631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="95" mass="10849" checksum="837B45B56CBF39E4" modified="2024-05-29" version="1" fragment="single">MTSYLQKELKEKRYKKCNIKGTSNSKLFHPDNLLGEASVAVDRCVKLMGYVAKVMFFYNKPMNSWSLKCLSNNLWREMFLGMIESGNTIGVISDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CNF5</accession>
    <name>A0AAD5CNF5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NPH3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_003894</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the NPH3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007395">
        <property type="protein sequence ID" value="KAI7744992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CNF5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043454">
        <property type="entry name" value="NPH3/RPT2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027356">
        <property type="entry name" value="NPH3_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32370">
        <property type="entry name" value="OS12G0117600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03000">
        <property type="entry name" value="NPH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51649">
        <property type="entry name" value="NPH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="NPH3" evidence="4">
        <location>
            <begin position="1"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="257"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7744992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="358" mass="40309" checksum="B3378954997B40DB" modified="2024-05-29" version="1">MHYAQKRLRGLEIFGKSKKKVDPKQKHEKRVVLETIVGLLPREKNAVSISFLSMLLRAAKYLDTTVACRLDLEKRMGLQLGQAVLDDILIPSTCFAGDTMFDIDTVQQIMINYFECNSTGLDDINYVSPLQSDDENVGKLMESFLAKIASDRNLLVSKFIGFAECIPIEVRVTEDGTYRAIDIYLKAHPGISSMDRKKVCSLMDCQKLSNEARAHAAQNERLPVQTMVQVLYYEQQRLREKDGSPMNNASSDPLKSRNMVLEEISRLKKENQELKFELIKMKTRLKEIEMSNDNKPKVNNGIGTMATAAKPPLPKKSLISSVTKTLGKITPFLYGDGFVASVTRGRNKAGKDRRHSVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CP77</accession>
    <name>A0AAD5CP77_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_027066</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007188">
        <property type="protein sequence ID" value="KAI7745606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CP77"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7745606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="57" mass="6472" checksum="D4833CC91B6BBFD0" modified="2024-05-29" version="1">MYHQTLVRCVCNHLLSVFSSHVDGYTSSLWIPSGQVGVLRFRYQGSRLRVQVLVCSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CPR5</accession>
    <name>A0AAD5CPR5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">M8C21_016235</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">AA19_3_7</strain>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007095">
        <property type="protein sequence ID" value="KAI7745853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CPR5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000537">
        <property type="entry name" value="Putative receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000178">
        <property type="entry name" value="serine/threonine-protein kinase PBS1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045874">
        <property type="entry name" value="LRK10/LRL21-25-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27009">
        <property type="entry name" value="RUST RESISTANCE KINASE LR10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 13" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="26"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAI7745853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="341" mass="38653" checksum="170979473BD3D8A0" modified="2024-05-29" version="1">MVQFLDDMEREKPIRFTSQQLKIATGSFSSILGSGGFGIVYKGIIGINKYVAVKVLTGTSNERIEEQFMAEVSTIGRTHHYNLVRLYGFCFEPSLIALVYEFMVNGSLDKHLFKANQGDMIGFDKLHDIALGTARGLRYLHEECAQRIVHYDIKPGNILLDSNFCAKVADFGLAKLCNRDKTHITMTGGRGTPGYAAPELWLPLPVTHKCDVYSFGMLLFEIIGRRRNMDNTLGDSDQWFPIWAWKRYEKNQLKDLMTNCEIEEKDREVVERMLKVALCCCQYRPETRPIMSIIVKMLEGVLEVPEPLNPFTHLFSGGNESNASVIQRSWNVSGSNWSSSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CPT2</accession>
    <name>A0AAD5CPT2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_017238</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007051">
        <property type="protein sequence ID" value="KAI7746028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CPT2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="388"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="274"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="274"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7746028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="441" mass="50178" checksum="144AF12ED90C76CA" modified="2024-05-29" version="1">MKDQEVKHYLYQVIDRVTFVQTPYKDKEQGGVLRTLGHRNGCLKAGGNMTRKETFKKEAITSRNEKEVATCIRDLNAPGKDMDRDLLAELLINLTKFQKEIITQDSLLGGERCTKSSIGCMLARLLLENGIPYNEVWLLIYEGGEAQGQLVEIEFRFDMCQHVLGITISLTEMNFHCSSTVARPFRNLWYFTIWVLTCQLHRKRVNTQPLHLSLSFSQNATSTHTNNICLTPCLNNKDRERETVKERLSTREMEKVRAFDVKLVQGGACSLHEENTTVEKERRETETGETSHSRRTPTAINTSTVTPIHDYPSSVTVSGGAQGSTILFHANSGGFRWAVEHKPWRSEVPWFFRWRLRKRVEKLREKGTGVRITVHLAAINRRKLRGMLVINDILVAVVVVVVAGVVVCKEKKEHRGSGKGRTDFVNVGGEYIDGNWEYGLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CQ22</accession>
    <name>A0AAD5CQ22_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_020361</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007331">
        <property type="protein sequence ID" value="KAI7745185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CQ22"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7745185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="90" mass="9889" checksum="F482D3E796E26EA4" modified="2024-05-29" version="1" fragment="single">CSSFLSRYFLPYLPIIINYTTTLPIITFSSPPENTTPFSTLPPRLRSKNSSSSLAAVAQQLRLYKPPAAAVDDYDDDDDDKVGFAESATP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CR15</accession>
    <name>A0AAD5CR15_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF1977 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_004685</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006949">
        <property type="protein sequence ID" value="KAI7746543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CR15"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015399">
        <property type="entry name" value="DUF1977_DnaJ-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09320">
        <property type="entry name" value="DUF1977"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="23"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1977" evidence="2">
        <location>
            <begin position="54"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7746543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="143" mass="16500" checksum="BEF355529D726AE3" modified="2024-05-29" version="1">MDGTIDVNDVAKSTKTSDRGGRLCFICCCFVLFCSSIILLVGYDYYTNTHYPYSVVVDDSYPFPLTTKKFGIEFYVKSRDKFDRKYAVGTSKRSKIEDKIIEDYLYYANNCCQDEQEWHQVRPDFPTPTCDKLQSLGINGSRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CS02</accession>
    <name>A0AAD5CS02_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">FAD-binding PCMH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_017586</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the oxygen-dependent FAD-linked oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006887">
        <property type="protein sequence ID" value="KAI7746837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CS02"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.462.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012951">
        <property type="entry name" value="BBE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32448">
        <property type="entry name" value="OS08G0158400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08031">
        <property type="entry name" value="BBE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="6">
        <location>
            <begin position="1"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7746837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="414" mass="46538" checksum="1621B6A4E46A76A3" modified="2024-05-29" version="1">MHLRFVVIDLSKLSGISVDIADGSAWVEAGATIGELYYRIAEKSKTHGVSAGICTSLGVGGHITGGAYGSMMRKYGIGVDNALDAKIVDANGRILDRKAMGEDAFWAIRGGGGGSFGVIVSWKLKLVAVPETVTVFTVGRTLEQGANKILYKWQQIGDKLDEDLFLRVLIQPTNVSNTTQRTITTSYNALFLGKANKLQDIMKQSFPELGLKKKDCIEMSWLESVLYMDFYPRTVPTSILLAGKATFVTYFKAKSDFVKHPIPGKGLEGIWQHLLKEDGPFMVFTPYGGIMNRIPESSTPFPHRNGTLFMIQYLTVWPNPLKKDLMEKHIDWIRKLYSYMAQYVSMNPRQAYLNYRDLDLGMNDKNGDDHTSFVKASTWGTKYFKENFNRLVKIKSEFDPDNFFKHEQSIPVSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CTZ8</accession>
    <name>A0AAD5CTZ8_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_031517</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006576">
        <property type="protein sequence ID" value="KAI7748231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CTZ8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7748231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="77" mass="8994" checksum="4F848F7A07816A5F" modified="2024-05-29" version="1" fragment="single">TYHELKKLKKLNERLIICHIFLFSLSQKGILGIVTMVVQIRNETLASVMKDPTHGMRQTLNNKWVFDEGLGYTKYKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CVH5</accession>
    <name>A0AAD5CVH5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ATP-dependent Clp protease proteolytic subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_012869</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec, and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs).</text>
            <dbReference type="EC" id="3.4.21.92"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the peptidase S14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006605">
        <property type="protein sequence ID" value="KAI7748080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CVH5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009368">
        <property type="term" value="C:endopeptidase Clp complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009532">
        <property type="term" value="C:plastid stroma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051117">
        <property type="term" value="F:ATPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07017">
        <property type="entry name" value="S14_ClpP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000035">
        <property type="entry name" value="ATP-dependent Clp protease proteolytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00444">
        <property type="entry name" value="ClpP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001907">
        <property type="entry name" value="ClpP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023562">
        <property type="entry name" value="ClpP/TepA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033135">
        <property type="entry name" value="ClpP_His_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10381">
        <property type="entry name" value="ATP-DEPENDENT CLP PROTEASE PROTEOLYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10381:SF8">
        <property type="entry name" value="ATP-DEPENDENT CLP PROTEASE PROTEOLYTIC SUBUNIT 6, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00574">
        <property type="entry name" value="CLP_protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00127">
        <property type="entry name" value="CLPPROTEASEP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00382">
        <property type="entry name" value="CLP_PROTEASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0720">Serine protease</keyword>
    <feature type="active site" evidence="6">
        <location>
            <position position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00034021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7748080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="268" mass="29018" checksum="26A9943FC64C4B58" modified="2024-05-29" version="1">MAASALSTSFTFSTVSSTSQRSSKLKSTVCALPTPYSDSPKLGLSSKAQNLQLTPEEINLHTPLNSSYSPIVARRGAPPPIMPAVMTPGGPLDLSTVLFRNRIIFVGQPINSQVAQRVISQLVTLATIDENADILVYVNCPGGSTYSVLAIYDCMSWIKPKVGTVCFGVAASEGALLLAGGEKGMRYAMPNARIMIHQPQSGCGGHVEDVRRQVNEAVQNRHKIDMMYSAFTGQPLEKVQQYTERDRFLSVSEALEFGLIDGILETEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CVM6</accession>
    <name>A0AAD5CVM6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_028208</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the plant LTP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006396">
        <property type="protein sequence ID" value="KAI7749153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CVM6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00010">
        <property type="entry name" value="AAI_LTSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.110.10">
        <property type="entry name" value="Plant lipid-transfer and hydrophobic proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036312">
        <property type="entry name" value="Bifun_inhib/LTP/seed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016140">
        <property type="entry name" value="Bifunc_inhib/LTP/seed_store"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043325">
        <property type="entry name" value="LTSS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33044">
        <property type="entry name" value="BIFUNCTIONAL INHIBITOR/LIPID-TRANSFER PROTEIN/SEED STORAGE 2S ALBUMIN SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14368">
        <property type="entry name" value="LTP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00499">
        <property type="entry name" value="AAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47699">
        <property type="entry name" value="Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042250641" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein" evidence="9">
        <location>
            <begin position="25"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical" evidence="10">
        <location>
            <begin position="33"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7749153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="189" mass="20434" checksum="CC78DFA385603FD5" modified="2024-05-29" version="1">MYNKLFVLVAVMVVWMVACRGVEAAEDSLAQECSKKLTAVMTCVDFATGKEATPQKKCCDSVKEMKDSNPACLCFVIQQIHNGTNPMLKSINIQESRLLQLSSACNIANASISDCPRLLKLPPNSPDASIFTNNATIVPPTSGRTPSSTTTSPLGSSRALKYKAPGILRSTWIPLIFLLVVSSKSVFYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CVN4</accession>
    <name>A0AAD5CVN4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Hydroxymethylglutaryl-CoA reductase (NADPH)</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_008302</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 3/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HMG-CoA reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006695">
        <property type="protein sequence ID" value="KAI7747739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CVN4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004420">
        <property type="term" value="F:hydroxymethylglutaryl-CoA reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015936">
        <property type="term" value="P:coenzyme A metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016126">
        <property type="term" value="P:sterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3270.10:FF:000002">
        <property type="entry name" value="3-hydroxy-3-methylglutaryl coenzyme A reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.770.10">
        <property type="entry name" value="3-hydroxy-3-methylglutaryl-coenzyme A Reductase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3270.10">
        <property type="entry name" value="HMGR, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.420">
        <property type="entry name" value="Hydroxymethylglutaryl-CoA reductase, class I/II, NAD/NADP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002202">
        <property type="entry name" value="HMG_CoA_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023074">
        <property type="entry name" value="HMG_CoA_Rdtase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023282">
        <property type="entry name" value="HMG_CoA_Rdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009023">
        <property type="entry name" value="HMG_CoA_Rdtase_NAD(P)-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009029">
        <property type="entry name" value="HMG_CoA_Rdtase_sub-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10572:SF43">
        <property type="entry name" value="3-HYDROXY-3-METHYLGLUTARYL COENZYME A REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10572">
        <property type="entry name" value="3-HYDROXY-3-METHYLGLUTARYL-COENZYME A REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00368">
        <property type="entry name" value="HMG-CoA_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00071">
        <property type="entry name" value="HMGCOARDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55035">
        <property type="entry name" value="NAD-binding domain of HMG-CoA reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56542">
        <property type="entry name" value="Substrate-binding domain of HMG-CoA reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50065">
        <property type="entry name" value="HMG_COA_REDUCTASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="85"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="121"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="13"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="121"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7747739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="313" mass="34586" checksum="FF7C9F028C7EEDE1" modified="2024-05-29" version="1">MDLRRKLPPRPPSITNNNNNKSVTVDRSTSPVPSPKASDALPLPLYLTNTIFFTLFFSVAYYLLHRWRDKIRSSTPLHVVTLSELTAIVSLIASFIYLLGFFGIDFVQSFISTSRGEEEEDGCFDNEVEDGSEQQQQQQPKPRFIDQVLISEEDEEIVKAVVSGSVPSYSLESKLNEDCYRAALIRRQAVERMTGRSLLGLGMEGFDYKSILGQCCEMPVGYVQVPVGIAGPLLVNGCEYMVPMATTEGCLVASANRGCKAIYCSGGATAILLKDGMTRAPVVRFATAERASQLKFFLEDGDNFDTLSVVFNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CX63</accession>
    <name>A0AAD5CX63_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Dihydroflavonol 4-reductase</fullName>
            <ecNumber evidence="8">1.1.1.219</ecNumber>
            <ecNumber evidence="7">1.1.1.234</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Dihydrokaempferol 4-reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Flavanone 4-reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">M8C21_021934</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">AA19_3_7</strain>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Bifunctional enzyme involved in flavonoid metabolism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>(2S)-flavan-4-ol + NADP(+) = (2S)-flavanone + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11228"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15605"/>
            <dbReference type="ChEBI" id="CHEBI:15606"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.234"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a (2R,3S,4S)-leucoanthocyanidin + NADP(+) = a (2R,3R)-dihydroflavonol + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54444"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:138176"/>
            <dbReference type="ChEBI" id="CHEBI:138188"/>
            <dbReference type="EC" id="1.1.1.219"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pigment biosynthesis; anthocyanin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the NAD(P)-dependent epimerase/dehydratase family. Dihydroflavonol-4-reductase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.219" evidence="8"/>
    <dbReference type="EC" id="1.1.1.234" evidence="7"/>
    <dbReference type="EMBL" id="JAMZMK010006341">
        <property type="protein sequence ID" value="KAI7749567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CX63"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045552">
        <property type="term" value="F:dihydroflavanol 4-reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047890">
        <property type="term" value="F:flavanone 4-reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009813">
        <property type="term" value="P:flavonoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08958">
        <property type="entry name" value="FR_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000085">
        <property type="entry name" value="Dihydroflavonol reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050425">
        <property type="entry name" value="NAD(P)_dehydrat-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366:SF709">
        <property type="entry name" value="CINNAMYL-ALCOHOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366">
        <property type="entry name" value="NAD DEPENDENT EPIMERASE/DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0284">Flavonoid biosynthesis</keyword>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="14">
        <location>
            <begin position="13"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00042087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00042831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAI7749567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="326" mass="36142" checksum="AA4830F30C780019" modified="2024-05-29" version="1">MNRQEESTKKTVVCVTGASGYIASWLVKFLLHRGYTVKATVRDLNDPKKTKHLLALDGAKERLHLFEADLLKDGSFDDVVKGCDGVFHTASPFFIHTDNPQEDLIDPAVKGTMNVLSSCSKVQSIKRVVLTSSVAAVLYNGSPLTPEVVVDESWFSDQEFCKETKLWYPLSKTLAEEAAWKYVKEKAMDMVSINPAMVIGPLLQPTLNTSARAIFNLINASTYSNITVGCVHVKDVANAHILAFETPSASGRYCMAESVVHFSELVQIIHKLYPSFKLPDKCAADDPVIKYQISNEKVKSLIGSDYISLEDGIKDTIESLKEKKYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CXM7</accession>
    <name>A0AAD5CXM7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_002113</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006196">
        <property type="protein sequence ID" value="KAI7750151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CXM7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009809">
        <property type="term" value="P:lignin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05283">
        <property type="entry name" value="CAD1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000022">
        <property type="entry name" value="Cinnamyl alcohol dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.180.10:FF:000004">
        <property type="entry name" value="probable cinnamyl alcohol dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.180.10:FF:000100">
        <property type="entry name" value="Putative cinnamyl alcohol dehydrogenase 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047109">
        <property type="entry name" value="CAD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42683">
        <property type="entry name" value="ALDEHYDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="7">
        <location>
            <begin position="16"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7750151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="658" mass="71748" checksum="7AD343DA01D8DFBE" modified="2024-05-29" version="1">MEGRKVTGWAARDSSGILSPYSFHLRKTGPEDVVFKVLYCGVDHTDLHQIRNEIHSTTYPLVPGHEVVGKVIEVGSEVKKFGVGDVVGVGCMVGSCGECVRCKTNREQYCNNMVFTYNAIYKDGSFTQGGFSSAMVVHQNYVVSIPEKLAPEQAAPLLCAGVTAYSPLKQFMGSKKAIKAGILGLGGVGHLGVLIAKAMGHHVTVISSSDKKREEALSDLGADCFLVSSNLAEMDKLRNSLDYILDTVPANHQLQDYLPLLDVEGKIFVVGAVPEPLQFSASGLILGKASISGSFIGSIQETQEILDFWVEKGLKTMIEVVKMDYVNKAFERMEKNDVRYRFVLDVAGSNLEKVIGWAARDSSGILSPYSFHLRKTSPEDVVFKVLYCGVDHSDLHQIRNEAHYTTYPLVGSEVKKFGVGDIVGVGCMVGSCGECTRCKTNREQYCNNMVFTYNAIYKDGSFTQGGFSSTMVVHQNYVVNIPEKLAPEQAAPLLCAGVTAYSPLKQFMGSKKAIKAGILGLGGVGHLGVLIAKAMGHHVTVISSSNKKRVEALTDLGADCFLVSSNSAEMDKFKNSLDYILDTVPVNHKIQDYLPLLDVEGKILMVGAVPEPLQFSASGLILVEKPLLQTYRKVLFYVNIWQPAYTESQTKRLDGTII</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CYT3</accession>
    <name>A0AAD5CYT3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">E3 ubiquitin protein ligase RIN2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">M8C21_025269</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">AA19_3_7</strain>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006074">
        <property type="protein sequence ID" value="KAI7750863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CYT3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034052">
        <property type="term" value="P:positive regulation of plant-type hypersensitive response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000259">
        <property type="entry name" value="E3 ubiquitin protein ligase RIN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003892">
        <property type="entry name" value="CUE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057992">
        <property type="entry name" value="TPR_SYVN1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15067">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15067:SF4">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02845">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25563">
        <property type="entry name" value="TPR_SYVN1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51140">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="80"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="127"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="176"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="216"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="14">
        <location>
            <begin position="295"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="CUE" evidence="15">
        <location>
            <begin position="488"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="450"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="450"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAI7750863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="528" mass="58638" checksum="C8A7F6E91B184E22" modified="2024-05-29" version="1">MGGVQAKLYYKGDHFTLYPMGVGYLPVSAVCTVLSFIGLQYWSELSYVKLKSDGLMGEDLLHPGVANRVLELLLGSYTTFALVVNFVLNIFMLIILSLKTIFFVELQAAESRKIVERLVNYVIYKGTFLPLVIPPTIVQAGLWSTWLTVLCSLKMFQALARDRLERLNASPSATPWTYFRVYSALLLVVSVDAVCFRNTSGSLWEWKSVILRNLGFFLDIMTLLMALGHYVYIWRLHGMTFHLVDAVLFLNIRALLSAIAKRIRGFVKLRIALRTLHGALPDATFEEIQAYDDECAICREPMAKAKKLACNHLFHLSLDQGLSDNYSCPTCRKPLFVGTPEGNSVPHSGDVSSDEQLARQLSSTLELQNVPTGVNPNQMQAPFERDDWRGSGPESGWLGFDGSDPSTAIRPAGLGRVQMMMRHLAAVGETYAQNALEDSSWNLWPINPSQASTSTSNNPSTSGIRHRGHAGGSLISTTPRVSDDDLSHLLAMAETVREVLPHVPDELILRDLQRTNSVSVSVNNLLQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CZH9</accession>
    <name>A0AAD5CZH9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_033958</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006262">
        <property type="protein sequence ID" value="KAI7749914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CZH9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000337">
        <property type="entry name" value="ABC transporter G family member 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050352">
        <property type="entry name" value="ABCG_transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041:SF56">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19055">
        <property type="entry name" value="ABC2_membrane_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="46"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7749914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="316" mass="34441" checksum="3B283AFC68B9238C" modified="2024-05-29" version="1">MVSTTQNMSPQHVIDPIMLQPSLPIQLQFIDICYKVKTHNNNDNNGVLKNMFKGASATNDNTVRERMILTGITGMVNPGEILAVLGPSGSGKSTLLNALAGRLSEDGFTGKVVANGRKLTKSVLKRTGFVTQDDVLYPHLTVRETLVFCALLRLPKSLSRKEKEEIADSVIAELGLCKCADTIIGNTFIRGVSGGERKRVSIAHEMLINPSLLILDEPTSGLDSTAAHKLMSTLGGLAHQKGKTVVTSVHQPSSRVFQSFDMVLVLSEGRCIYFGKGSEAMSYFESVDFRPCFPMNPADFLLDLANGTFYIYSHVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CZQ5</accession>
    <name>A0AAD5CZQ5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_026657</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006050">
        <property type="protein sequence ID" value="KAI7750944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CZQ5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd12203">
        <property type="entry name" value="GT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000061">
        <property type="entry name" value="Trihelix transcription factor GT-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044822">
        <property type="entry name" value="Myb_DNA-bind_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21654">
        <property type="entry name" value="FI21293P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21654:SF90">
        <property type="entry name" value="TRANSCRIPTION FACTOR MYB FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13837">
        <property type="entry name" value="Myb_DNA-bind_4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="181"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="124"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="273"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="143"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="276"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7750944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="505" mass="57989" checksum="22AD250199E911D1" modified="2024-05-29" version="1">MIVKKLGGFVGVGEKQGYGRMVVEVVNEYGDGLFKTKKVGVANRWCERVMAMRRWSCPVGLRCLRGVRRVGLLRCRIQTPPHTTNAMDHSRFCQPAFTSGDPRLHFPSLVSEVPFYMHQVLNPNAPPPSTPPSHTFLDSTTVSPPPPPPPTYSPQTGWNAYSGGINLNQGTSGYAQGTTVRWPRQETLTLLEIRSRLDHCFKEATSSNHKAPLWDEISRIMKEEYGYQRSGRKCKEKFENLYKYYKKTKEGKVGKTDGKHYRFFRQLEALVGNQHEPGSTNTNTAVYPSNQSNHSSGNNTPSNHHHEFNKKEMIAAIEESVESHFMKLMVKQEQWCEKILSTIEQKEQERMMIENEWRKQEGARLDQEYEAWASQVARIKTRDESLLKALQNLIKVRSVESKETDQSLSGHEKGKDWIEPEIASLIHIRTTKEGKFDDQDVDQQHEDRLWQEVAFEMSLLGYNRSDKVCKETWDKLCVENRISREVSSGTSNPMNIDCGRSWETM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D0L8</accession>
    <name>A0AAD5D0L8_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_006767</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005962">
        <property type="protein sequence ID" value="KAI7751157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D0L8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7751157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="74" mass="8433" checksum="BCB6EF719C22664A" modified="2024-05-29" version="1">MTMAMDFVREIGGSELLGPPRFRQDSDRDLMMVDGERRLLVMIQMTVSGGGDRRQQLQFCAEFMTVDGFLESIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D2B1</accession>
    <name>A0AAD5D2B1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_028332</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005765">
        <property type="protein sequence ID" value="KAI7752072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D2B1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7752072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="202" mass="22520" checksum="0C489954881E8E8D" modified="2024-05-29" version="1">MLGLDAKSCVRVSCTWQSDREFHYGLHTEFEMQALLHACIACSLKPSIEWIASSDLQDESAKSACKPESVGAKFLKGYSTKSAVLSRCGHGCCSAPQTSLLGPEFVEYDEVPALSNIELATIAADLNSIAWIKRGLKIPQTQSSLKEMKKQNRGNYSAWVFIFLSRARLQMYAFYQGVCATSFTMLIICYNSKVISEHDYYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D2R1</accession>
    <name>A0AAD5D2R1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="2">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_030421</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010005771">
        <property type="protein sequence ID" value="KAI7752010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D2R1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045191">
        <property type="entry name" value="MBR1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22937">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF165"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22937:SF222">
        <property type="entry name" value="RING-TYPE E3 UBIQUITIN TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="10">
        <location>
            <begin position="384"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="34"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7752010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="430" mass="47600" checksum="9C26402B527A04BA" modified="2024-05-29" version="1">MQRIRGMFYLGLHLLAHRTSPVHQAPPALHYTIVEPGNETHPEDLPPTTQDSTQQVDDEDLSSDTLTPDFHDPIPPECTAEECEEEEPDEENVEEIVTGIPSIPDMAILTSIQRDHPIENVIGPVNVGVLTRSQTVAVEHQIVIRLGSSGVDPMNSEENRAAGAVGFEFDPLMVSDGSHMVRGGQLVQGVPAGGYSWNRAHGVPYLQDSAFHGYQVAAMGRNSAAGMHPHQVSYNVYHPPPPSPQMLPPPPPPMLPIQAQNLDIQLQQPSTSHRHSTRPSHMYPFQSDAPVPRFVGPALPHGAMVYEARRQQFMINSISRRRSFSHHRVTLEEAVQALREQLGQAVDSGLPAKFIAEHLKTRTFTSSRHHSQDLTPADEEPNLCVICQMELEDQEKVGTLGCSHEYHAKCIKKWLTVKNNCPVCKSTALR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D2X5</accession>
    <name>A0AAD5D2X5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">U4/U6.U5 tri-snRNP-associated protein 2-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_023111</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005760">
        <property type="protein sequence ID" value="KAI7752100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D2X5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000245">
        <property type="term" value="P:spliceosomal complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02669">
        <property type="entry name" value="Peptidase_C19M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000068">
        <property type="entry name" value="U4/U6.U5 tri-snRNP-associated protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033809">
        <property type="entry name" value="USP39"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001607">
        <property type="entry name" value="Znf_UBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF16">
        <property type="entry name" value="U4_U6.U5 TRI-SNRNP-ASSOCIATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02148">
        <property type="entry name" value="zf-UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00290">
        <property type="entry name" value="ZnF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50271">
        <property type="entry name" value="ZF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBP-type" evidence="13">
        <location>
            <begin position="71"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="12">
        <location>
            <begin position="193"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="34"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7752100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="516" mass="60382" checksum="BD8268FAC51D4EA6" modified="2024-05-29" version="1">MEESREYDEEQEAASKKQKLGFDNALLPLAAYEDKEEDESEDEEEEEEDSEEEEYNMMNRNRRRRTIEVRRDCPYLDTVNRQVLDFDFEKFCSVSLSNLNVYACLVCGKYFQGRGPNSHAYTHSLEAGHHVFINLRTEKVYCLPDSYEINDPSLDDIRHLLNPRFHIHQVLQLDLNRQWSRALDGSDYLPGMVGLNNIKDTDFVNVTIQSLMRVTPLRNFFLIPDNYSHTKSLLVHRFGELTRKIWHPRNFKGQVSPHEFLQAVMKASKKRFRIGAQSDPVEFMSWLLNTLHTDLKTSRTKSGSSSIIHQCFQGELEVVKEINANDGAETSRMPFLMLGLDLPPPPLFKDVMEKNIIPQVPLFNILKKFDGESVTEVVRPRVARMRYRVTRLPQYLILHMRRFTKNNFFVEKNPTLVNFPVKNLELKDYIPLPSKEEEEEKVRSKYDLIANIVHDGKPGEGSYRVFVQRKSEELWYEMQDLHVAETLPQMVALSEAYMQIYEQQQQSDSQKQVDAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D381</accession>
    <name>A0AAD5D381_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NB-ARC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_020035</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005444">
        <property type="protein sequence ID" value="KAI7753383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D381"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11017">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11017:SF313">
        <property type="entry name" value="TIR DOMAIN, P-LOOP CONTAINING NUCLEOSIDE TRIPHOSPHATE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NB-ARC" evidence="1">
        <location>
            <begin position="54"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7753383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="180" mass="20299" checksum="5EE01BB9D56B00E7" modified="2024-05-29" version="1" fragment="single">LWERALTQVADLKGKAVNGRKETKLIEEVVTDIHRRIGVPLCHTLPSLQGRNYDIRLITSWLTDGSCHTIDILTIVGISGIGKTSLAKYVFRLHFGKFHKSSFIEDINTRCKEHHNGLLDLQKQLHGDISKKIELHVNDVTMYTSKIESVLSRKRVFIVLDDIGSLDQLNALLGKNGLHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D4Y6</accession>
    <name>A0AAD5D4Y6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_020399</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005745">
        <property type="protein sequence ID" value="KAI7752151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D4Y6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20654">
        <property type="entry name" value="CYP82"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000026">
        <property type="entry name" value="Cytochrome P450 82C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050651">
        <property type="entry name" value="Plant_Cytochrome_P450_Monoox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47947">
        <property type="entry name" value="CYTOCHROME P450 82C3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47947:SF19">
        <property type="entry name" value="CYTOCHROME P450 82C3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7 8" id="KW-0349">Heme</keyword>
    <keyword evidence="5 7 8" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 8" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="7">
        <location>
            <position position="463"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7752151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="525" mass="59294" checksum="3B6D39BB710700AD" modified="2024-05-29" version="1">MNYSLEIIAFYISLSFFIYVIFTRRKNLKSSAGEAPELGGAWPIIGHLHLLGGGDQLLYRTLGGMADKYGPAFNIRLGTRRAFVVSSWEVARECFTTNDKALASRPKTAAVKHMGYNYAVFGFAPYTPFWREMRKIATLELLSVRRLDMLKDVRSLEITSGINELYRRWTENLQQPMVVELNKWLEHMMLNIVVLMVAGKRYFGAGGGGDEAERCQKAISEFFRLIGIFMVSDAIPFLSLLDLQGYEKEMKKTAKDLDLVLSGWVDEHRQKRKLCVEHDKEDVKDFIDVMLSLEDEGQLENMEHDSDTSIKSTCLALILGGSDTTAGTLTWAISLLLNHPNVLKKLQHELDEHVGKERQVDESDIKNLVFLHAVIKETLRLYPAGPLLGPREAVEDCTVAGYNVKAGTRLIVNIWKLQRDERIWSDPYAFKPERFMAGSDHEHVDLKGQQFVLMPFGTGRRSCPGATFGLHVLHLTLARLVHSFDMGQPGGLLVDMTESPGMTIPKKKPLEVILTPRLPAELYGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D5K3</accession>
    <name>A0AAD5D5K3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein-tyrosine-phosphatase MKP1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_021419</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005380">
        <property type="protein sequence ID" value="KAI7753585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D5K3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004721">
        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14498">
        <property type="entry name" value="DSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057528">
        <property type="entry name" value="MPK1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46381">
        <property type="entry name" value="MKPA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46381:SF4">
        <property type="entry name" value="PROTEIN-TYROSINE-PHOSPHATASE MKP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25466">
        <property type="entry name" value="MPK1_gelsolin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="4">
        <location>
            <begin position="193"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="5">
        <location>
            <begin position="252"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="43"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="83"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="140"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="558"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="54"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7753585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="884" mass="96875" checksum="BE0A09E146C9016B" modified="2024-05-29" version="1" fragment="single">SHTLIFAKPESSILLLPLSFRLLIPSRFHPLGMVGKEDDDCGGDGINGETSRLNSNNNNSNNNISGNRKMYWRSASWSSSHVSLPPLVPDGVKDGGDVNGGLSRRRPGPLTPRSNSKGRSCLPPLAIARRSLDEWPRAGSDDIGEWPIPSTPSGRDVNGNGERLKLDLSSIQRVPDRNPGLVRRDKIAFFDKECSKVDEHIFLGGDAVAKDKEILKQHKITHILNCVGFVCPEYFKGNFVYRTLWLQDSPSEDITSILYDVFDYFEDVRDQGGKVFVHCCQGVSRSTSLVIAYRMWREGQSFDDAFQYVKAAREIADPNMGFACQLLQCQKRVHAFPLSPSSLLRLYRIAPHSSYDPLHLVPKMLNVPSPGALDSRGAFIMHIPSTIFIWVGKKCESLLERDARGAVCQIVRYEKVQGPIVVVKEGEEPSYFWDAFSSLLPLMDKSGNIMDIGKFSNVSPGERIVDSYNVDFEIIQKAIVGGFVPAFASSETDQETHLPARESSWSVLRRKFAYGNMKEFVLDSKSAPSTVYPDSVLLCSENQTSFPFKPVISSSTSSLSSSSSLSPSFSSASSPDSISSDSTISSKCYSDSPVASPSVSSHTLALSSTVSTLSNLSLVPTKLSPHSISKSSEFIDVNFASTNSCSQSVLSPSKRYSLSIAERRGGTPKCLKLPMLSDNSQGQDACLVIEPNKDIVLELKEKGPNQEHIQGSCSSCFSEVDGAEFAQTSKALQGNQSLKRKDGESAHHNVKKEVVVLEWPSLDQIMGLCSGDMDSSSEKDGASVVYIWVGKAFRHDSWNLLDTNKRLSDLPDFSMKEAIKKVLSEMGLSKDTHIELPGVAVRCGAAFPNSPVSHFCCSNNISVKDAGQNSENVSGIQLQTRKPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D6R7</accession>
    <name>A0AAD5D6R7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_033118</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005069">
        <property type="protein sequence ID" value="KAI7753919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D6R7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000327">
        <property type="entry name" value="Cysteine-rich receptor-like protein kinase 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000560">
        <property type="entry name" value="Putative LRR receptor-like serine/threonine-protein kinase isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052059">
        <property type="entry name" value="CR_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47973">
        <property type="entry name" value="CYSTEINE-RICH RECEPTOR-LIKE PROTEIN KINASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="26"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="95"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="394"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="420"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7753919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="430" mass="49332" checksum="5EEC2E03CE0184BA" modified="2024-05-29" version="1">MCRLLQEEPVTFDQQSSYTKPHKTRLFILVGGLILFLVFLIVIMAFRKYIRTPQLGKCVNRKDSCKGSAKDYFSGKLETISYFSFRTLKEASKNFHECNLLGKGGFGPVYRGKLQDGRLVAIKKLSFEKSHQGEAEFLAEVRMITSIQHKNLVRLLGCCCEGPQRVLVYEYMKNRSLDRIIYGKSNQYLNWKTRFQIILGIARGLQYLHEESHLRIVHRDIKASNILLDGNFQPKIGDFGLARFFPEDQAYLSTTFAGTLGYTAPEYVIRGELSEKADIYSFGVVVLEIISCRRNTDLTLSSEMQYLPEYAWKLYERSKMIDLIDPRMQKDGFSVRDVMKTIHVALLCLQPLPVIRPAMSEIVAMLTWKVEMVKSPIKPTFLDRRRRNNDENVSCETISNDFPSPLEAESPSLTQPPNSREFDAKGEIIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D6U7</accession>
    <name>A0AAD5D6U7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_006837</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010003621">
        <property type="protein sequence ID" value="KAI7754424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D6U7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000162">
        <property type="entry name" value="Adenine nucleotide alpha hydrolase-like domain kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47989:SF14">
        <property type="entry name" value="INACTIVE PROTEIN KINASE SELMODRAFT_444075"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47989">
        <property type="entry name" value="OS01G0750732 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="461"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="239"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="326"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="363"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="254"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="331"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="375"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="675"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7754424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="675" mass="74938" checksum="DD60951F442013CF" modified="2024-05-29" version="1" fragment="single">MHNIDEQKPRIIAGDEGQCSRLSYEAKSPMPSPPRPLLAFNQMTSLVKHFMFRSVAYLDKEVIKSAEFASLKMWYMIRNILAKFGEYERTPVRVLIPLQVFYIYRNTSQKSLFPDFGGIFLRVVIFVSGSMSREEKKMGKAENGSDAAAEKIVVAVKASKEIPKTALVWALTHVINVKIKIVSGSPCGAVAAEAKKIQASWVVLDKKLKHEQKRCMEDLQCNIVVMKKSQPKVLRLNLVGSPKKKPEADCPLMPSTNQSSEKQTKNKNSSLDSIRGPVVTPTSSPEVFTATEAGTSSVSSSDPGTSPFFVPVTNSLLKMGKSLVGKESQDLNESSSDSESEHLSSTSSSLRFQPWMVDMITSSCQSSQHKEESSQRYTSLETSRSQDMFRRLSKLDRDSEHGSHRSDVEFNGNVREAISFSRNIPSGPPPLCTICQHKAPIFGKPPRWFTYAELELATGGFSQANFLAEGGFGSVHRGILPDGQVVAVKQHKLASSQGDQEFCSEVEVLSCAQHRNVVMLIGFCIEDGRRLLVYEYICNGSLDSHLYGRHRDPLEWAARQKIAVGAARGLRYLHEECRVGCIVHRDMRPNNILITHDFEPLVGDFGLARWQPDGDTGEETRVIGTFGYLAPEYAQSGQITEKADVYSFGVVLVELVTGRKAVDLNRPKGQQCLTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D6V0</accession>
    <name>A0AAD5D6V0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PRONE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_026766</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010002234">
        <property type="protein sequence ID" value="KAI7754953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D6V0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.2010:FF:000003">
        <property type="entry name" value="Rop guanine nucleotide exchange factor 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1310:FF:000001">
        <property type="entry name" value="Rop guanine nucleotide exchange factor 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2010">
        <property type="entry name" value="PRONE domain, subdomain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1310">
        <property type="entry name" value="PRONE domain, subdomain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005512">
        <property type="entry name" value="PRONE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038937">
        <property type="entry name" value="RopGEF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33101:SF79">
        <property type="entry name" value="PRONE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33101">
        <property type="entry name" value="ROP GUANINE NUCLEOTIDE EXCHANGE FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03759">
        <property type="entry name" value="PRONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51334">
        <property type="entry name" value="PRONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRONE" evidence="4">
        <location>
            <begin position="13"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="155"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7754953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="332" mass="37341" checksum="E217588FEC2B18DC" modified="2024-05-29" version="1" fragment="single">MEEDNADANSELDPELESIYADMELMKERFAKLLLGEDMSGGGKGVSSALALSNAITNLAASIFGEISRLEPMPTESKARWQKEIDWLLSVTDHIVEFVPSKQNTNGVTMEIMITRQRSDVNVSIPALKKLDWMLMECLDSFRDQNDFTYVSKGEVGSKSSRRREEDKCGLPTPKVPSNGLPDLTKKWLQFQRDSVNQLLKAAMAVNAHILTEMDIPENYIDSLPKNGRASLGDTVYKNITVDHFDPDVFLAAMDLSSENKILDLKNRIEASVVIWRRKMTNKDGKSGWGSGVSIEKREQFEDRAETILLILKHRYPGLFQSALDASKIEHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-06-18" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D756</accession>
    <name>A0AAD5D756_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GIL1/IRKI C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_014888</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010004440">
        <property type="protein sequence ID" value="KAI7754144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056813">
        <property type="entry name" value="GIL1_IRKI_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042316">
        <property type="entry name" value="IRKI-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31029">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31029:SF4">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24994">
        <property type="entry name" value="GIL1_IRKI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GIL1/IRKI C-terminal" evidence="3">
        <location>
            <begin position="378"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="130"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="35"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7754144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="447" mass="50305" checksum="237043F66D6783B1" modified="2024-05-29" version="1">MAASSSSLSPLPHLHPPFTPIQESETEGTEENGSCQLTSANRPESHNSGTKSDDRGVLLNKCRPSSREKFSVVPLDNTAAGRHSNASPNGLFKSILSTFKKSPRPGSALSEKLTPVPXXXXXEEQWKSAVAELSNKLIQATKKRDEAVLEASRLKFSMVELEKKLNKLELYCKNLKSGLDDCANKASNMNYEPIKIGDHERVIEHFLVSVSESRTSMRHLSRSLTVQLPQIDGGKLYDRIQLLLQPYEIKVSGSTNPRILLLYLEALLNRAFFEDFESPGFRKSGANRVLNPIHRCEENFKSFMRLKELSWEEVLNNGTKQFSEEFSKFCDRKMSEVVGMLGWTRAWPEQLLQAFFGASKAVWLVHLLANSVHPGLPIFRVDRGVRFDPVYMEDMGGDKARRFMSPTVRLMVEPGFYIYGNVVKCKVICRYHSNNGYISVSPSSTPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D8S6</accession>
    <name>A0AAD5D8S6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_030961</name>
        <name evidence="3" type="ORF">M8C21_030966</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010002083">
        <property type="protein sequence ID" value="KAI7755027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAMZMK010002083">
        <property type="protein sequence ID" value="KAI7755032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D8S6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7755027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7755032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="88" mass="9940" checksum="FDCA64C7802ED6FE" modified="2024-05-29" version="1" fragment="single">MRLANDDGETPRMNSDATRPGGPVERDIEQAITALKKGAYLLKYGRRGKPKFCPFRLANDESVLIWFSGKEEKHLKLSHVSRIVSGQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DA87</accession>
    <name>A0AAD5DA87_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">M8C21_019202</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">AA19_3_7</strain>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the splicing factor SR family. RSZ subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000140">
        <property type="protein sequence ID" value="KAI7757291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DA87"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd12373">
        <property type="entry name" value="RRM_SRSF3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000214">
        <property type="entry name" value="Serine/arginine-rich splicing factor 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050907">
        <property type="entry name" value="SRSF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23147">
        <property type="entry name" value="SERINE/ARGININE RICH SPLICING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RRM" evidence="13">
        <location>
            <begin position="2"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="14">
        <location>
            <begin position="98"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="66"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="112"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="74"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="125"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00061011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAI7757291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="224" mass="24765" checksum="387815C0FC17933B" modified="2024-05-29" version="1">MSRVYVGNLDPRVTERELEDEFRSYGVLRNVWVARRPPGYAFVEFDDRRDALDAIRALDGKNGWRVELSHNSKGDGGGRGGRGGGGDRGGRGGGDDLKCYQCGEPGHFARECRNRGGSRGFGGGGRRRSPSPRRRRSPIATALATVLVVGGHHHLGVAYHLAEAAATAGRRPRTAVPDVFPLMLMESKMEIIRDGWRLLNVRSSPEKRPFVVFYFLHYTASRGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DB88</accession>
    <name>A0AAD5DB88_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Methyltransferase-like protein 22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_026768</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010002234">
        <property type="protein sequence ID" value="KAI7754955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DB88"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008276">
        <property type="term" value="F:protein methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019410">
        <property type="entry name" value="Methyltransf_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038899">
        <property type="entry name" value="METTL22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23108:SF0">
        <property type="entry name" value="METHYLTRANSFERASE-LIKE PROTEIN 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23108">
        <property type="entry name" value="METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10294">
        <property type="entry name" value="Methyltransf_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7754955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="246" mass="27680" checksum="CD8F2B8A8AC5D447" modified="2024-05-29" version="1">MVSDVEYIEGKYEYQVFDDSKRPQDVSLDDDGDLALPRRKKHPKDKFDVAIQHNITSSIPKVGLQVWRAELVLCDFVLHKMYTSSEFDGIVAVELGAGTGLVSMLLARVAKTVFLTDHGVDILENCACNVHLNSALLPSKTSVYVRELDWKASWPPKQDNGASCESQSEFKELERASLLVAADVIYSDDLTDALFHTLERLMSHGIKKVLYLALEKRYNFSIDDLDVVANGYSCFRSYVRDAKGKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DBM0</accession>
    <name>A0AAD5DBM0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CP-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_009968</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000275">
        <property type="protein sequence ID" value="KAI7756737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DBM0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01855">
        <property type="entry name" value="YqeH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030378">
        <property type="entry name" value="G_CP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044229">
        <property type="entry name" value="NOA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048422">
        <property type="entry name" value="NOA1/YqeH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47569">
        <property type="entry name" value="NO-ASSOCIATED PROTEIN 1, CHLOROPLASTIC/MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47569:SF2">
        <property type="entry name" value="NO-ASSOCIATED PROTEIN 1, CHLOROPLASTIC_MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21516">
        <property type="entry name" value="YqeH-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51721">
        <property type="entry name" value="G_CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CP-type G" evidence="2">
        <location>
            <begin position="180"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7756737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="563" mass="62105" checksum="EC1E17EBD4D7708E" modified="2024-05-29" version="1">MIPKTLSSLTSFSHPFLHPQFKIPKSYNPGRVIIICNNKSINPQPQQQTQPELSLPGSEPQGTGAAAPTRGDIYLERQQLLRSQSLVIEKKKKKKKEKANGSLKVATFCCYGCGAPLQVQEMDAPGYVDPDTYDLKKKHRQLRTILCGRCRLLSHGHMVTAVGGNGGYAGGKQFVTAEELREKLRHLRHEKALIVKLVDIVDFNGSFLAHVRDLAGANPIILVVTKVDLLPKGTDFNCVGDWVVEATMRKKLNVLSVHLTSSKSLVGITGVVSEIQKEKKGRDVYILGAANVGKSAFINAILKMLSDKDPVAAMARKYKPIQSAVPGTTVGPIQINAFLGGGKVYDTPGVHLHHRQAAVVHSQDLPALAPRSRLRGQSFTKEVVLDQWVSEKMEFNSLTGFSIFWGGLVRIDVLKVLPETCLTFYGPKAVKLHIVPTSKADEFYKKELGGLLTPPSGEQVEDWAGLETARQLRITFDDTQRPACDVAISGLGWITVEPVSQALKDPDADLMPEAPVISLDVHVPKAVEIFVRPPLPVGKFGGEWYQYRDLTEKEEETRPKWYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DCI7</accession>
    <name>A0AAD5DCI7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">WAT1-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_028472</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the drug/metabolite transporter (DMT) superfamily. Plant drug/metabolite exporter (P-DME) (TC 2.A.7.4) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000185">
        <property type="protein sequence ID" value="KAI7757072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DCI7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030184">
        <property type="entry name" value="WAT1-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31218">
        <property type="entry name" value="WAT1-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="15"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="45"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="81"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="110"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="142"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="202"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="264"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="291"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="7">
        <location>
            <begin position="20"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="7">
        <location>
            <begin position="200"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7757072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="350" mass="38395" checksum="BB8B7464990D725D" modified="2024-05-29" version="1">MAIERSQTWSWVDDAVPFVVMLMLTCLDMSLLTVVKAAMNGGLGSIAYVVYHNSLGTLILFPILVFRMLRNVERPPLTIHILFRFFVLGLCGLCLFQLLVYVGVNYTSPTLASAITNLIPGNTFLFAIVFRMEKIDIRSSSTIAKLMGTMMAIFGAMVFTFYQGPEIFVTISSPNSPNNRLLLSELSNWSATARGYPDQNTIVFFYCLFGTIQCIALSPFLEPNPSAWALQPGVGMNAVVLGAIYSTAFHSNAQTWCLKKKGPVYVAMFSPLSIVIGVILGVTFLGDSLHLGSAIGIAMITVGFYIVMWRQAKEKNMLVAMIDEDIVVANELGSSDQNTPLLSCINESKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DDW5</accession>
    <name>A0AAD5DDW5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_018733</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000036">
        <property type="protein sequence ID" value="KAI7758012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DDW5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7758012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="61" mass="7014" checksum="4502F410A46E2560" modified="2024-05-29" version="1" fragment="single">VVNEPNRRLFTLQKLYGNIPRGGRVSKRQFKSKTRHLKKLALSKQLLPRKKRNSGGAALMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G224</accession>
    <name>A0AAD5G224_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">ADP-ribosylation factor GTPase-activating protein AGD11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_014643</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011991">
        <property type="protein sequence ID" value="KAI7725247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G224"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08204">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04038">
        <property type="entry name" value="C2_ArfGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.150:FF:000009">
        <property type="entry name" value="stromal membrane-associated protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044518">
        <property type="entry name" value="ARF_GAP_AGD11/12/13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46220:SF2">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR GTPASE-ACTIVATING PROTEIN AGD11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46220">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR GTPASE-ACTIVATING PROTEIN AGD12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01412">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00405">
        <property type="entry name" value="REVINTRACTNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00105">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50115">
        <property type="entry name" value="ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Arf-GAP" evidence="8">
        <location>
            <begin position="111"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="7">
        <location>
            <begin position="279"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="13"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7725247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="448" mass="49663" checksum="E3E0B24AA713A0AA" modified="2024-05-29" version="1">MSTTEKKKDSENVLANTKRANSQLGDNKESCQKAQMSMTEKKKDSENVAANTKAKNSKPGDDKESCQNLTKAQMATEEKKKVFENVSANAKEKNSQLGENLESCQKLTSPQEKLAGFISESGNMVCADCGTPDPKWVSVSFGAFICIKCSGAHRSLGVHISKVLSVNLDEWTHKEVNIVINCGGNAMVNSKFEATIPEKCKKPKPDSSIEERFDFIRRKYEMQEFADTSKEICLGPHPSMHCISTNFVGAIMDKKNSNNIRAHNIGQAFRNSWKKKEVENKTTRKSNSMAGMVEFIGLIKVNVVKGTNLAVRDMLTSDPYVILSLGNQSMKTRVIKSNLNPVWNEKLMLSIPNNIPHLKVLVYDRDTFKADDFMGDAEIDIQPLVYAAKASENSEVDESTMPHGKCTESKDGVITISKGIVKQEIALKLQNVEKGVLQIELECVPLTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G2I7</accession>
    <name>A0AAD5G2I7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">BYPASS-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_009625</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the ROH1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011734">
        <property type="protein sequence ID" value="KAI7726175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G2I7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008511">
        <property type="entry name" value="ROH1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31509">
        <property type="entry name" value="BPS1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05633">
        <property type="entry name" value="ROH1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7726175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="363" mass="40515" checksum="2BAAF4EE931894B7" modified="2024-05-29" version="1" fragment="single">RFIQLLPPQTQPETKPEPETETILSISWFRKLLDVYLCCESEFKAVLIIGRDASCFSKPPLDRVLPEHLDRTVKALDICNAVTHGIEMIRHWLRLAQIAVDALEQRPIVDGHVRRAKRALNALLMEMVGEDKEPNAPVGKLAERMWSISRRGGSAVFPPPTTVNHTRKISVSRSLSSSFSKSWSASKQLQAMSTNLVAPRGGDPTGLSLPVYLMSSVLVFVMWTLVTAVPSQERVGPGTNFQLPRHLVWNNPMIALQEKIAEDWKKKEKRGAAGLLAEVQVMGKVAQNLVEFADGFEFPVGVEKVDEVAAQVTELSDICRELEVGLGPLQLQVRELFHRMVRSRAEVLDVMDIVGKINTNVPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G3N0</accession>
    <name>A0AAD5G3N0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Histone H2B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">M8C21_026485</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">AA19_3_7</strain>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the histone H2B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011472">
        <property type="protein sequence ID" value="KAI7727002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G3N0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22910">
        <property type="entry name" value="HFD_H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000014">
        <property type="entry name" value="Histone H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000558">
        <property type="entry name" value="Histone_H2B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055333">
        <property type="entry name" value="HISTONE_H2B_site"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23428">
        <property type="entry name" value="HISTONE H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00621">
        <property type="entry name" value="HISTONEH2B"/>
    </dbReference>
    <dbReference type="SMART" id="SM00427">
        <property type="entry name" value="H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00357">
        <property type="entry name" value="HISTONE_H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5 12" id="KW-0158">Chromosome</keyword>
    <keyword evidence="9 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="11 12" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="10 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="14">
        <location>
            <begin position="36"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAI7727002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="139" mass="15292" checksum="1770438996A5D425" modified="2024-05-29" version="1">MAPKAAEKKPAEKKPAEKAPAEKKPKAGKKLPKEAGAADKKKKRSKKSVETYKIYIFKVLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSRLARYNKKPTITSREIQTAVRLVLPGELAKHAVSEGTKAVTKFTSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G5L2</accession>
    <name>A0AAD5G5L2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">60S ribosomal protein L39</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_021997</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL39 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010969">
        <property type="protein sequence ID" value="KAI7729579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G5L2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1620.10:FF:000001">
        <property type="entry name" value="60S ribosomal protein-like L39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1620.10">
        <property type="entry name" value="Ribosomal protein L39e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000077">
        <property type="entry name" value="Ribosomal_eL39"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023626">
        <property type="entry name" value="Ribosomal_eL39_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19970:SF0">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19970">
        <property type="entry name" value="RIBOSOMAL PROTEIN L39E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00832">
        <property type="entry name" value="Ribosomal_L39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48662">
        <property type="entry name" value="Ribosomal protein L39e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7729579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="49" mass="6040" checksum="0547266801CFDDE8" modified="2024-05-29" version="1" fragment="single">VTQDIHDQEEACQEDEAERPIPTWIRMRTDNKIQYNAKRRHWRKTKLGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G5Y6</accession>
    <name>A0AAD5G5Y6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CAAX prenyl protease 2/Lysostaphin resistance protein A-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_033796</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011157">
        <property type="protein sequence ID" value="KAI7728741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G5Y6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004175">
        <property type="term" value="F:endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080120">
        <property type="term" value="P:CAAX-box protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003675">
        <property type="entry name" value="Rce1/LyrA-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43592">
        <property type="entry name" value="CAAX AMINO TERMINAL PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43592:SF4">
        <property type="entry name" value="CAAX AMINO TERMINAL PROTEASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02517">
        <property type="entry name" value="Rce1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042045193" description="CAAX prenyl protease 2/Lysostaphin resistance protein A-like domain-containing protein" evidence="2">
        <location>
            <begin position="23"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="119"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="160"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="CAAX prenyl protease 2/Lysostaphin resistance protein A-like" evidence="3">
        <location>
            <begin position="209"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7728741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="284" mass="31759" checksum="017E273E0E4F66AA" modified="2024-05-29" version="1">MITTRLHLSLPFLCLTSSPSIPTSIRTFQNNRILHSTSIYKCRDSSFKLYCTTNNNNNNNKSSQDFSILKTDIQCDSGSTWSTMAFYIFSLHIPLSFGGLSVVAQLLHQTVLDPNTQALSLLLIQTLELVLFLSLLKFSNNGFNVLSLFETRVLPKNRNWLLAAVLGVGFLLALVFLTSLIADKLVGPKDVNNPILKEILSGGPISVTGSTLVYCLVTPLLEETVYRGFLLTSLASTMKWEQAVVHKPPFLFTKDEMVKSGMRLEMMTLIHASDYRLFIWLTAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G655</accession>
    <name>A0AAD5G655_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_024082</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010912">
        <property type="protein sequence ID" value="KAI7729807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G655"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23022">
        <property type="entry name" value="zf-HIT_DDX59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007529">
        <property type="entry name" value="Znf_HIT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48453">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48453:SF1">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04438">
        <property type="entry name" value="zf-HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="271"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="36"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="64"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7729807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="450" mass="50745" checksum="520BF9628793382F" modified="2024-05-29" version="1" fragment="single">MGTRTNFYKNPSFTYNKDFDLNSALQNLKAYNAVTGNPTLPEQKPVSGDRKVFSQPNRRKRRRNNPDPDRRPDVQETDGPMSHHDYIQKIRKEANSSQYIEELTTDVLESSTAGGQLVGYDSKFKMMLVLAFCDKSSSSEGEENRVSGSIQNVYEEKKNPNRDGNIDAANLVKERSQQRFAVPGEPVCVVCGKYGEYICDETDDDICSMDCKAELLENLKFSLGPRSNQSLVEPSRGPKFPLQVFESGGDTWDFNRHRWSTKRSSLSAYECVKCKKAGHLAEDCLVMNWNPQSFPFGNQASSSSNGSSVIPKDLLELYKRCHQIGKTSAAAKCNACHRVSSLAACLDCRISFCDSGGHLMEHIRTHPSHRKYYSFKLNRLVKCCKSSCKVTDIKDLLACHFCFDKAFDKFYDMYNATWKVAGISIIASSICCEDHFAWHRMNCLNAGVED</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G6U7</accession>
    <name>A0AAD5G6U7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_005785</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010727">
        <property type="protein sequence ID" value="KAI7730572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G6U7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7730572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="83" mass="9198" checksum="81A53E8EF209C2DC" modified="2024-05-29" version="1" fragment="single">DLSFNNLSGKIPSSFSLLGNQNPVHIYLTNNDLTGPMPRWVRSSTKNVDLCYNHFTWDASAPDNCEQGTVNVVESYSSSTSEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G711</accession>
    <name>A0AAD5G711_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_032486</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010588">
        <property type="protein sequence ID" value="KAI7730929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G711"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000400">
        <property type="entry name" value="Nuclear pore complex protein NUP107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046959">
        <property type="entry name" value="PRK1-6/SRF4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48007">
        <property type="entry name" value="LEUCINE-RICH REPEAT RECEPTOR-LIKE PROTEIN KINASE PXC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48007:SF50">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="287"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="401"/>
            <end position="661"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7730929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="677" mass="74744" checksum="188ACC24829FB787" modified="2024-05-29" version="1">MCTLPKPFHHHHHHHHNHHHHHHLFSLLIILTISLPTTISTTLPSDAVSLLKFKLKADINNNLLYNLNERFDYCQWQGVKCVQGRVIRFVLQNHNLTGTFADNTLSNLNHLRVLSLRNNSLTGPIPNLSPLTNLKTLFLDHNLFTGSFPISLLSLHLLQSLDLARNNLSGSLPVQLTSLDRITNLRLEFNRFTGSLPPLNQTTLLVFNVSGNNLTGPVPATPTLSKFDASSYLLNPNLCGKIVNKICDERSPFFHAPVTAAPAPVVENGGAEGLVVAPPGSRKHKRVGIIILGLVIAVFIVVALVVTVVGVLNKRRYGSRTKSVAFSSDDEIGTERDNGTAVAAVLTDTATAVPAVGKESKVSIKVREQKLELPQQHREKSGNLIFCEGERAMYGLENLMSASAELLGRGTIGTTYKAVMDNQLIVTVKRLDAGKTAVTSGEAFEKHLEAVGGLRHPNLVPVRAYFQAKQERLVIYDYQANGSLFNLIHGSRLTRAKPLHWTSCLKIAEDIALGLAYIHQASRLIHNNLKATNVLLGSDFEACLTDYCLLSLADPTAIDESISAGYKAPELRKSFHRATTKSDVYAFGVLLLELLSGRPPSQHPHLAPEDMAEWVRAMRENDDLLNDRRLEMLVEVAGVCSMTSPEQRPVMRQVLKMLQEIKETASNDDDNTYKGYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G7G9</accession>
    <name>A0AAD5G7G9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">LMBR1-like membrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_033178</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LIMR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010357">
        <property type="protein sequence ID" value="KAI7731920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G7G9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051584">
        <property type="entry name" value="GPCR-associated_LMBR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006876">
        <property type="entry name" value="LMBR1-like_membr_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21355">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR-ASSOCIATED PROTEIN LMBRD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21355:SF14">
        <property type="entry name" value="LMBR1 INTEGRAL MEMBRANE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04791">
        <property type="entry name" value="LMBR1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="30"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="236"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="348"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="543"/>
            <end position="565"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7731920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="565" mass="64219" checksum="31F7EA67221443B5" modified="2024-05-29" version="1">MDKLFGYVTVLLVHMELRWQDVLVDRSGGIMGFAMACSNTFGLVTGAFLLGFGLSEIPKGIWINADYITRQKVLSHKVARAALKLDDAHQNLSNAIVVAQATSKQMSKRDPLRPYMDIIDKMLMNMLNEDPSFKPQGGRLGENDMDYDTDEKSMATLRRQLRIAKAEYYRSKSEYMNFVMEALELEDTLKSYEHRDATGWKYISSFRPERPGKIGLCLDRVELVWRCIMRKQLKKLSAIILGCMTAAILLAEATILPSGVDFSLFSILINAVQTNEVLVQIDFLLTYSKSNKFSQPFDDMLVARYAPPISYNFLNLINLPRNATTVFERRMGKIDDAVPFFGQNFNRIYPLIMVVYTVMVASNFFDRIMSYFGNWKIFRLGNEEEADDLDGFDPSGLMILQKERSSISRGHKVGEFVIPLARYFNDTTTDIESGSNNMFASNSNSTLSIEEGTSNKYKALRTGQTVTQPSSDINVATDTDPIMRNEQQSRITSTWMSMKTNLQNFKSNMAARRFMPLRQSQDLQEPQHQDSSESLDEIFARITKAPRNDSEDLDSYAVGPSRHGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G8W7</accession>
    <name>A0AAD5G8W7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phloem protein 2-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_021483</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010397">
        <property type="protein sequence ID" value="KAI7731741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G8W7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025886">
        <property type="entry name" value="PP2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32278">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32278:SF149">
        <property type="entry name" value="PHLOEM PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14299">
        <property type="entry name" value="PP2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7731741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="502" mass="58810" checksum="86EDFC0275B263BE" modified="2024-05-29" version="1">MLSGELADNQGSLSSSAKNYYEQGKLDELVFGGIKEQIMPQSLSTFADIAYKCLHQDRHQRPTAGEVVTQLQEAQEFQWLPRDYKEIIQQSRTPEIYKTQKKMDLYDMLREGILIQKGKVWFSLDNNNKRNEMISATLFTYQNQSSQKWRNNRKSRFQRVSLMMDISNLMIQAKIKAQFLTPSVTYGVYLVFKFVEPTKISSKPKYVNLKYKMGNETLHAYFATWRKDKWMMIELYRFLSNKDGVDFEVLLESFSKCYCGNGRIYVEGIEFRAIDNVTHEEIEKSKDAQQIMRSNSNIDLMQQFPADKDEILGVTENSDEAPKPSEMNGKKHHMLSAKEVLYDSSNVKLFKLKPSEQSRFGEVIEVLSQQVFRIKCKIESQILSPDTDYRCYLIFKLSEKCRGLHCPVKVRSVHRPVKVEGQLHQSRKETKDLYFRTPKEWNIHDINQVPKQREDGWMEVNVWKFNSKDQLRNDFIHLNLKLISYEGTMSGLIVCGLEFRPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G9U0</accession>
    <name>A0AAD5G9U0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_028602</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009933">
        <property type="protein sequence ID" value="KAI7733659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G9U0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045015">
        <property type="entry name" value="AN-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43254:SF3">
        <property type="entry name" value="C-TERMINAL BINDING PROTEIN AN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43254">
        <property type="entry name" value="C-TERMINAL BINDING PROTEIN AN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding" evidence="2">
        <location>
            <begin position="138"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="367"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="401"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="377"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="401"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="425"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="444"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7733659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="584" mass="64295" checksum="FEBAB335520B2FD1" modified="2024-05-29" version="1" fragment="single">MMSSSSTVKHRDLETRSPTTMSHRNNPTPHSSLPLVITLNCIEDTSIEHQYLTNIARVDHVPLSHLTDAKIESASAVILHSLVYLPRAAQRRLRPYQLVLCLGSADRAVDSAIASDLGLSRFVHVDVCRDEEVADTVMALFLGLLRRTHLLSRHALSASGWLGSVQPLCRGMRRCRGLVLGIVGGSGSAKALATRSLAFKISVLYYDVVHQEKGKRISRFPPAARRMDTLNDLLAASDLISLHCSLTNDTIQIINAECLQHVKPGAFLVNTGSSQLLDDCAVKQLLIDGTLAGCALDGAEGPQWMEAWVREMPNVLILPRSADYSEEVWMEIREKAASILHTFFLDDVIPTDAVSDIDEEESDMLYEAEQSDKQAKHSASSSSNIHQLTESIRISHETQMKNDVNQPQESTLSQNTSSKSEVKHSRSSKKAKKRHARQKSMQKVGDKESTPHKEDDTAMSGTDQALSSSSHFASPDDSRSKRTPIFGTIHDSSAKYLKSSTGVGKNSLELLKDGCVISLQARDRAALHVSRQRAQGGGWFLDTMSNVTKRDPAAQFLLVFRSKETIGLRSFTAGGKLLQKLQNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GA37</accession>
    <name>A0AAD5GA37_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_021657</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009611">
        <property type="protein sequence ID" value="KAI7734935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GA37"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7734935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="118" mass="13522" checksum="90C5EC9893D18FCE" modified="2024-05-29" version="1">MEQMYRVRTAELKNDLEGYIMPLAKSLILKLGDFHMFIFCVDGLLFCPSRQRIYFLALYDLKAVKDFLVHDCYTLPDDYNLALALDTRPVEPMEAKALVAISSILTKVRPSSSGDIWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GBE1</accession>
    <name>A0AAD5GBE1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_022705</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009328">
        <property type="protein sequence ID" value="KAI7736255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GBE1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43329">
        <property type="entry name" value="EPOXIDE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7736255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="379" mass="43275" checksum="BCD8F8FC6DC93051" modified="2024-05-29" version="1">MVAYTFSLIHPEKVAGVVTLGVPFMPPGFFKSHQALPEGFYMKRWQEPGRAEADFGRFDAKTVVRNIYIMFSRSEIPIAGEDQEIMDLVEPSTPLPSWFTEADLSAYGDLYQKSGFQTALKVPYRSFEEKTGSPQQGVNDPKVEVTALLIMGEEDYVLKFPGMEEYLKSGEVKKYVPNLEIVYLPQGCHFVHEQFPDQSHQALPEGFYMRRWKEPGRAEADFGRFDAKTVVRNIYIMFSRSEIPIAGEDQEIMDLVDPLDPLPSWFTEDDLAVYGELYHKSGFQTALQVPYRSFAEKMGSPQQGPNDLKVEAPALLIMGEKDYMTKLPGAEEYLKSGEVKKYVPNLEIVYLPEGCHFVHEQFPDQVNQLVLNFLEHYKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GDN9</accession>
    <name>A0AAD5GDN9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">FAD-binding PCMH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_033234</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oxygen-dependent FAD-linked oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009297">
        <property type="protein sequence ID" value="KAI7736363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GDN9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.43.10:FF:000004">
        <property type="entry name" value="Berberine bridge enzyme-like 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.462.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.10">
        <property type="entry name" value="Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012951">
        <property type="entry name" value="BBE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016167">
        <property type="entry name" value="FAD-bd_PCMH_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32448">
        <property type="entry name" value="OS08G0158400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08031">
        <property type="entry name" value="BBE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="8">
        <location>
            <begin position="59"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7736363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="528" mass="59822" checksum="40C415ACA7BAD6B2" modified="2024-05-29" version="1">MSSDQTHKIYASFFQCLQQISGEPDPNISSILYSSTINAASYTTVLQSHIRNRRFNNVSTPKPTLIITPTKESHVQAVVICAKKLGVHLLIRSGGHDYNGLSYVSHEKTFILLDMYNLHNVSVDISTETAVVEAGAHLGELYYRIGEKSKVHGFPAGVCPTVGVGGHISGGGYGTMIRKYGLSVDHVVDARIVDAEGRVLDRKSMGEDLFWAIRGGGGASFCIVLSYTLKLVHVPKINTVFRIKKTIEENAVDLVYKWQFVAPTTDRDLFLRVILMPETVNNKKTVQATFIAHFLGDSNKLLKIMNKSFQELKVKKDDCLEVSWVESALFWANMDHKTPHEVLLDRHLYTAQYLLRKTDYVQTLIPKHGWESIFNKLVELGKCGFYLNPYGGIMEEIKADATPCPHRAGNLFKIQYWLNYSEDDPELHEKYLNQTRVLHEFMTKFVSKNPRGVFLNYRDLDNIGVMVQTVESGYNTGRVYGEKYFMGNFDRLVKVKTAVDPDNFFRFAQSIPTLPKTLRADVRPFSRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GEJ5</accession>
    <name>A0AAD5GEJ5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">4-coumarate--CoA ligase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_015842</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008797">
        <property type="protein sequence ID" value="KAI7738564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GEJ5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031956">
        <property type="term" value="F:medium-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04433">
        <property type="entry name" value="AFD_class_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.980">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43201:SF32">
        <property type="entry name" value="2-SUCCINYLBENZOATE--COA LIGASE, CHLOROPLASTIC_PEROXISOMAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43201">
        <property type="entry name" value="ACYL-COA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="1">
        <location>
            <begin position="159"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="2">
        <location>
            <begin position="409"/>
            <end position="502"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7738564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="557" mass="61798" checksum="EEDB35D1EAD1499F" modified="2024-05-29" version="1">MPGSYSDPHICLCLTRLSTFRRHSPVSISGSRVKTGKEFVDGVVDLASGLINIGIKSGDVVAISAFNSDLYLEWLLAITYVGAIAATLNYRWSLEEALLAMEDVKPVMLVTDNEYAIQAHSVPSLRWHVLMDTNNRYSSTENILTTSMLKMPSPRRHSFNYTSNCDGVAIVCFTSVLKKNVYLHTAPLGHVGGLSSALTMLMVGGCHVLMPKFEAKLALEAIEEYHVTSFITVPTIMSDIISLIRTKYTRKELPMVKKILNGGGSLSTNQIKDATDIFPNAGLFTAYGMTEGCSSLTFMTLKDPTKQTTVEEELAVDTQHEGICVGKPAPHVELKIRTEDSAHAGQILTRGPHLMIGYWGQMHMEENHGNEGWHETGDIGRIDDHGNLWLIGRMKGRIKSGGENIYPEEVEAVLLRHPGISAIAVIGLPDNRLTEMVVACIQLNHNWTWTGSGSDPLGIKKQQTISSETLQQFCRTNNLTGFKVPRRFIRWSKKFPLTTSGKLRRDQLKIYKVAIYGIYAGIEVDKLMSLKRRLGGSRNMNKSSMTKLSHIGWAMKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GFH9</accession>
    <name>A0AAD5GFH9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_007546</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008860">
        <property type="protein sequence ID" value="KAI7738163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GFH9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7738163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="109" mass="12790" checksum="8EB74841B5CDE47C" modified="2024-05-29" version="1">MLKETMQKLVVENESSHEKGSQETNIICWSYALNFDDGTYHHHHEDNHHQHHHVVEHYKITKLPLEKQQESLVLSTTTTTTTWVVRLFEPGLTAIIRILQWVLSHDPTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GG45</accession>
    <name>A0AAD5GG45_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Jacalin-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_008700</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the jacalin lectin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008229">
        <property type="protein sequence ID" value="KAI7741265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GG45"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09612">
        <property type="entry name" value="Jacalin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.100.10.30">
        <property type="entry name" value="Jacalin-like lectin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001229">
        <property type="entry name" value="Jacalin-like_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033734">
        <property type="entry name" value="Jacalin-like_lectin_dom_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036404">
        <property type="entry name" value="Jacalin-like_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46506">
        <property type="entry name" value="OS05G0143600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01419">
        <property type="entry name" value="Jacalin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00915">
        <property type="entry name" value="Jacalin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51101">
        <property type="entry name" value="Mannose-binding lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51752">
        <property type="entry name" value="JACALIN_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0430">Lectin</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Jacalin-type lectin" evidence="3">
        <location>
            <begin position="3"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7741265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="145" mass="15417" checksum="CE10B21A894CE9E0" modified="2024-05-29" version="1">MESIEVGPWGGKGGGLWMQTSFGGPFKGISIRAGACIFSIYFTYTFKDNDNVETRSGGFGELGDNAEKITFADDEEIIGFSGTVGDWNGITCISSLTFQTNKKVYGPFGTVTDSEFSLPVKSGRFAGFFGNSSDVLDAFGAVLVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GHD0</accession>
    <name>A0AAD5GHD0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_004176</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008542">
        <property type="protein sequence ID" value="KAI7739963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GHD0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000029">
        <property type="entry name" value="Cytochrome P450 734A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050665">
        <property type="entry name" value="Cytochrome_P450_Monooxygen"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24282">
        <property type="entry name" value="CYTOCHROME P450 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24282:SF269">
        <property type="entry name" value="CYTOCHROME P450, REVERSE TRANSCRIPTASE, RNA-DEPENDENT DNA POLYMERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11" id="KW-0349">Heme</keyword>
    <keyword evidence="8 11" id="KW-0408">Iron</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="499"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="530"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="11">
        <location>
            <position position="462"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7739963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1188" mass="135728" checksum="0DC08002578CAD92" modified="2024-05-29" version="1">METIRKVAMAIVAAVILSWGWKLLNWVWLKPKKLEKWLRDEGYKGNPYKLLMGDLVELATMIKEGKSKPIPVTHDITSYTLPFDHHIISKYGEKSFVWMGPKPMLYIMDPDLIKEILSRPDEFQKPHPEPIRDSIIRGLLVAEGDKWTKHRKIINPAFSLQSLKIMFSAVISSCSNMIHKWELLTTGASSTEIDVWPYIDNLGGDMISRTAFGSSYEEGWKIFRIQKEQINLLFQMLFILYIPGSRFIPTQANKKFKENVNELRSVLMGIINKRKKAIEMGEGNHDDLLGLLLESNKKEIEEHGVGMSMEDVIEECKLFYIGGSETTSNLIVWTMVCLSLHQEWQTRAREEIVQVFGDKELEFDGLKNLKILTMILNEVLRLYPPVVLITRATHKNTKLGNMVMPSGMELALAMMHVHHDREIWGDDVNEFKPERFAQGISSATKGKGSSPFFPFSNGPRVCIGQNLAMTEAKTTMAMILQRFSFELSPSYKHSPFRAFTLPPQFGAHLILLSLFLVLEEKLRKNKMETMGKVAIACVVAMIVSWGWKLLNWVWLKPKRLEKWLRKEGYKGNSYKLLMGDMIELATIVNEGKSKASPITHDITSYALPFDHHIISKYGEKSFIWLGTIPRVYIRDPDLIKEILLRPDEFQKPHPEPFRDSIIRGLLVTEGHKWTKHRKIINPAFNLQNLKIMFSAISSSCSDMVRKWELLTAGAGPTEVDVWPYIDSLAGDVISRTAFGSSYEEGHKIFRIQKEQIDLIFQMLFILPIPGRRFIPIRANKKFNENVNELRSVLMGIINKRKKAIELGEGKHDDLLGILLESNKKEIEEHGVGMSMDDIMFSAISSSCSDMIHKWELLTAGAGSIEVDVWPFIVNLTGDVISRIAFGSSYEEGQKIFRIQKEQIDLIFQMFSILYLPGRRFIPTRANKKFNENKKELRSVLMAIINKRKKAIDMGEGKDDDLLGILLESNNKEIEEHGVGMSMEDVIEECKLFYIAGSETTSNLIVWTMVCLSLHQEWQIRARDEIFQAFGIDQPDFEGLKNLKVLTMILNEVLRLYPPAIMMTRATHKDTKLGNMMIPSGVQLALCVVHVHHDREIWGEDVNKFNPERFSEGIVNATRGRGSSPFVPFSNGPRFCIGQNLAMMEAKTAMAMVLQRFSFELSPSYKHSPFPVFTLPPQFGAHLILHNIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GHV1</accession>
    <name>A0AAD5GHV1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_032960</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008344">
        <property type="protein sequence ID" value="KAI7740816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GHV1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040305">
        <property type="entry name" value="At1g75730-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34792">
        <property type="entry name" value="OS02G0121500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34792:SF1">
        <property type="entry name" value="OS02G0121500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7740816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="334" mass="36486" checksum="1F6729EB79C061C3" modified="2024-05-29" version="1">MRKGSTGFKDGTRQSGGGSFVISHKASKKLKRLSALAGLDNKPTCSKRVKLPKNLSDNGHMVNLVSVPRKSRSVMKKRFADLSSPSRTSAISRCEMPSTDGNKKVKLDPDEEQSVMKNEEAAIAGLLALAGNDRTDEIKLNNKISEATGSKAEDLVQECVQFHDLNESSNTLDVKKDCKDVTVGNESRKRCASHVYICQVIKDLKMTKGKMVISCEEAKGVTAPKVVMNVNSGINLNEAIPFSSNTIEGQPTGCGVQSYFGNPYCDPSQWSRPMFPNQQMWMNPFMYKPLQSGSYVQASLHNVLGSKHNSSLGSDESAMFRVDSSPLTLKLALQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GJK2</accession>
    <name>A0AAD5GJK2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phosphoglycerate mutase-like protein AT74H</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_018133</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007333">
        <property type="protein sequence ID" value="KAI7745167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GJK2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001345">
        <property type="entry name" value="PG/BPGM_mutase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052765">
        <property type="entry name" value="PGM-Related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46192">
        <property type="entry name" value="BROAD-RANGE ACID PHOSPHATASE DET1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00175">
        <property type="entry name" value="PG_MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="1">
        <location>
            <position position="74"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="1">
        <location>
            <position position="162"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="73"/>
            <end position="80"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7745167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="337" mass="39094" checksum="B3A3739E92B22E1C" modified="2024-05-29" version="1">MNIPQLTPQTPTSPCTVMSITASSSIKCCEDTVGRRKRLSIDDTGLLTFPEKNLILHVPTAKPPRPRRIILVRHGQSEGNVDEGAYTRVADPKIRLTERGKREAEECGVRIREMIEKDGADDWKVYFYVSPYERTIETLRGLGKAFQRSRIAGVREEPRLREQDFGNFQDREQMKIQKAVRVQYGRFFYRFPNGESAADVYDRITGFRETLRTDIDIGRFQPPGEQSPNMNLVIVSHGLTLRVFLMRWYKWTVEQFERLNNMRNGNEIVMQTGQGGRYSLLVHHTQQQMIDFGLTQQMLDDQIWQKTAKPGELNYDYLTNGPSFFTHFDDENEAIFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GKE9</accession>
    <name>A0AAD5GKE9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_016278</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007018">
        <property type="protein sequence ID" value="KAI7746200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GKE9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030126">
        <property type="term" value="C:COPI vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050844">
        <property type="entry name" value="Coatomer_complex_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19876">
        <property type="entry name" value="COATOMER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19876:SF68">
        <property type="entry name" value="COATOMER SUBUNIT BETA'-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7746200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="252" mass="27816" checksum="E014A240DCA511B9" modified="2024-05-29" version="1">MSGERESINSKRKKGNGSGGGTGHTLRSTTFLAFLSIQSTDGGSNQRASALKPLRLDIKRKLAQRSERVKSVDLHPTEPWILKRLFSGTVNIWDYQAQVHLKSDSHFEFSDVHNDSATIATREIDDVKCQDDESKEDISRINEPVSFDEVSPSNNSKSTADDFLEIINPPITSEAIKDPATTNGHQISCQLDLGDAYKLAISTKGRQLSGKLLDQKSFNNSSARIGKDLKILLSHRSNDSLISPSYLLTVMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GKF7</accession>
    <name>A0AAD5GKF7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">P-type Ca(2+) transporter</fullName>
            <ecNumber evidence="2">7.2.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">M8C21_031072</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">AA19_3_7</strain>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>Ca(2+)(in) + ATP + H2O = Ca(2+)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18105"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.10"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.10" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010007551">
        <property type="protein sequence ID" value="KAI7744294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GKF7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005388">
        <property type="term" value="F:P-type calcium transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000021">
        <property type="entry name" value="calcium-transporting ATPase, endoplasmic reticulum-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000014">
        <property type="entry name" value="Calcium-transporting ATPase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000028">
        <property type="entry name" value="Calcium-transporting P-type ATPase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000065">
        <property type="entry name" value="Sarcoplasmic/endoplasmic reticulum calcium ATPase 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42861">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="13" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="83"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="113"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="286"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="326"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="792"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="886"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="971"/>
            <end position="990"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1002"/>
            <end position="1021"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1041"/>
            <end position="1061"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="18">
        <location>
            <begin position="26"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="19">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAI7744294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="1080" mass="118090" checksum="696085B485D2E1C2" modified="2024-05-29" version="1" fragment="single">CVLMGKGAQNYGKKESAGEGSSSSYAPWAKEAHECEEKYEVDRKNGLSDIEVEKRLKIHGYNELEKHEGQSVFRLILDQFNDTLVRILLVAAVVSFVLAWYDGEEGGEMEITAFVEPLVIFLILIVNAIVGVWQESNAEKALEALKEIQSEQATVIRNGRKHSGLPAKELVPGDIVELRVGDKVPADMRVLNLVSSTLRLEQGSLTGESEAVSKTTKPVPAESDIQGKKCMVFAGTTVVNGNCICLVTDIGMNTELGKVHSQIHEASQSEEDTPLKKKLNEFGETLTMMIGLICVLVWLINVKYFLSWEYLDGWPTNFKFSFEKCTYYFEIAVALAVAAIPEGLPAVITTCLALGTRKMAQKNALVRKLPSVETLGCTTVICSDKTGTLTTNQMAVAKLVAMGLTENAVRLFNVEGSTYNPLDGKIQDWPAGKMDANLQTIAKIAALANDASIERSEKGIVAGGMPTEAALKVLVEKMGLPSGLDSGSSTGDSDLMGCSRAWDKIERRIATLEFDRDRKSMGVIVSSNTGEKTLLVKGAVENLLERSSHIQLLDGSVVELDQRSKLVILDSLNEMSTGALRVLGFAYKDDPSEFRSYNGEEDHPAHNLLLDPTSYSSIESNLTFAGLAGLRDPPRKEVRQAIEDCRAAGIQVMVITGDNKNTAEAICREIGVFGPDEDISSKSITGRQFMEHHDPKSHLAQKGGLLFSRAEPRHKQEIVRLLKDAGEVVAMTGDGVNDAPALKLADIGIAMGIAGTEVAKEASDMVLADDNFSTIVAAVGEGRSIYNNMKAFIRYMISSNIGEVASIFLTAAIGIPEGLIPVQLLWVNLVTDGPPATALGFNPPDKYIMKKPPRRSDDSLINAWILFRYLAMSSDYCMLNLQVISFYVVIGLYVGLATVGIFVIWFTHDSFLGIDLSGDGHSLVTFSQLRNWDQCKSWENFTVSPFQAGDQLFNFESDPCDYFRTGKVKAMTLSLSVLVAIEMFNSLNALSEDESLLTMPPWVNPWLLLAMSISFGLHFLILYVPFLAQVFGIVPLSVNEWLLVLAVALPVILIDEILKFVGRLTMSGQRSSKSSKHKAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GMM0</accession>
    <name>A0AAD5GMM0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PCI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_007593</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CSN7/EIF3M family. CSN7 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006526">
        <property type="protein sequence ID" value="KAI7748435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GMM0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008180">
        <property type="term" value="C:COP9 signalosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045237">
        <property type="entry name" value="COPS7/eIF3m"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15350:SF17">
        <property type="entry name" value="COMPLEX SUBUNIT 7A, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15350">
        <property type="entry name" value="COP9 SIGNALOSOME COMPLEX SUBUNIT 7/DENDRITIC CELL PROTEIN GA17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22061">
        <property type="entry name" value="CSN7_HB_subdom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="3">
        <location>
            <begin position="1"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="228"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7748435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="249" mass="28344" checksum="BA5FA9993685DFD0" modified="2024-05-29" version="1">MDIEQKQEEFIQHFVNQASSSKASSLATVIADATSHPSLFAFSEILSLPNVIQRLSATVPAYRTIIRGNKYNMSGNASNLPPLVPDQVLKLKQLTVLTLAEANKVLPYDVLMQELDVINVRELEDFLINECMYVGIIRGKLDQFRRCFQVQFAAGRDLRPGQLGSMIHTLSNWLSTSDNLLITIQDKIKWADTMSELDKKHKKEADEKMEEVKKSLSLKEDIDFRGHEEMFPEPGGVMDYDEDRSRPKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GMQ7</accession>
    <name>A0AAD5GMQ7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_008506</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006924">
        <property type="protein sequence ID" value="KAI7746664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GMQ7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23798">
        <property type="entry name" value="UBCc_UBE2I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000038">
        <property type="entry name" value="SUMO-conjugating enzyme SCE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="5">
        <location>
            <begin position="5"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="3">
        <location>
            <position position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7746664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="160" mass="18044" checksum="394EDDFE81F5E70C" modified="2024-05-29" version="1">MSGGIARGRLTEERKSWRKNHPHGFVAKPETLPDGSVNLMIWHCTIPGKTGTDWEGGYYPLTLHFTEDYPSKPPKCKFPPGFFHPNVYPSGTVCLSILNEDSGWRPAITVKQILVGIQDLLDSPNPADPAQTDGYHLFIQDTVEYKRRVRLQAKQYPPLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GMS1</accession>
    <name>A0AAD5GMS1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_000124</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006501">
        <property type="protein sequence ID" value="KAI7748585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GMS1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000157">
        <property type="entry name" value="ATP/GTP-binding protein family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025224">
        <property type="entry name" value="CCAR1/CCAR2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14304:SF17">
        <property type="entry name" value="CELL CYCLE AND APOPTOSIS REGULATOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14304">
        <property type="entry name" value="CELL DIVISION CYCLE AND APOPTOSIS REGULATOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7748585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="112" mass="13130" checksum="8DB12BB0BFCF7969" modified="2024-05-29" version="1">MDEDPEEDPEEDEDMIDETENLKNEQEVNATEEIVNPSIKSGDEKEQKDETGKDKVFRFGHIRVEDMWLIIHNLGKFLSHRDVKDLIQSALLESNTGRDDRILYNKLVRMDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GQP3</accession>
    <name>A0AAD5GQP3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Homeobox-leucine zipper protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">HD-ZIP protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Homeodomain transcription factor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_013399</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Transcription factor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 10">Belongs to the HD-ZIP homeobox family. Class I subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005881">
        <property type="protein sequence ID" value="KAI7751540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GQP3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000159">
        <property type="entry name" value="Homeobox-leucine zipper protein HAT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045224">
        <property type="entry name" value="HDZip_class_I_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000047">
        <property type="entry name" value="HTH_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003106">
        <property type="entry name" value="Leu_zip_homeo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24326">
        <property type="entry name" value="HOMEOBOX-LEUCINE ZIPPER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24326:SF497">
        <property type="entry name" value="HOMEOBOX-LEUCINE ZIPPER PROTEIN HAT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02183">
        <property type="entry name" value="HALZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00031">
        <property type="entry name" value="HTHREPRESSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 8 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 8 9" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6 8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 10" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="12">
        <location>
            <begin position="58"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="8">
        <location>
            <begin position="60"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="131"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU369038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7751540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="268" mass="30445" checksum="78F4BDC9D93FC070" modified="2024-05-29" version="1" fragment="single">MESGRLVFGSKSSNMLFLGDGDNYFRGTRMAFNVEESAKKRSFLSSLDDMLEEEYYDEQLTEKKRRLSPEQVHMLEISFEEDNKLEPERKTELAKKLGLQPRQVAVWFQNRRARWKTKTLERDYNRLKSCYDVLTSDYESIVKENEKLKAEVVSLTEKMQTKEVSETFIVGQGLSSAAEAARDVPALLDNVKVEDHFSTGSGGSAVVDDHEGPQLVDSGDSYFLDDDYPHEYVVPVDQSEEDDGSDDGQNQFGYAFETVDHEEGDGLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GRQ1</accession>
    <name>A0AAD5GRQ1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_019095</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006223">
        <property type="protein sequence ID" value="KAI7750071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GRQ1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7750071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="113" mass="13139" checksum="39B0A105A9DCF106" modified="2024-05-29" version="1">MGTDESNLKVNVVLCDGVNEKFQKNRDKSALSSLVSYGYMPTLPKLSQHFFCISLRRGRVATYKGIVKRHQSYSRLEIPLKWIWFYTFRDGGTLGKDMDTEMNIRCLNDQKRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GS26</accession>
    <name>A0AAD5GS26_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Heme O synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_004894</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006146">
        <property type="protein sequence ID" value="KAI7750506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GS26"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008495">
        <property type="term" value="F:protoheme IX farnesyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006784">
        <property type="term" value="P:heme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.140">
        <property type="entry name" value="UbiA prenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006369">
        <property type="entry name" value="Protohaem_IX_farnesylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044878">
        <property type="entry name" value="UbiA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43448">
        <property type="entry name" value="PROTOHEME IX FARNESYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43448:SF2">
        <property type="entry name" value="PROTOHEME IX FARNESYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="109"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="134"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="176"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00030253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7750506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="202" mass="21435" checksum="287272BD5C24BFC7" modified="2024-05-29" version="1">MWRRRTNSLSQLTQLATSKFHPHLNNNIINNNIIIQSTSLRLFQITDANNGYGLLISRSSSSAVEASSVSPKIGFSGSAVDTTSLGLRGVADVVRHYGRCYWELSKAKLSMLVVATSGAGYVLGSGSAVDLAGLGYTCMGTMMVAASASTLNQVFEVKNDAMMNRTRRRPLPSGRITVPHAVTWASSVGLAGIALLASKVGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GSM3</accession>
    <name>A0AAD5GSM3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Apple domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_031886</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005403">
        <property type="protein sequence ID" value="KAI7753550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GSM3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01098">
        <property type="entry name" value="PAN_AP_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.90.10.10">
        <property type="entry name" value="Bulb-type lectin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001480">
        <property type="entry name" value="Bulb-type_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036426">
        <property type="entry name" value="Bulb-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035446">
        <property type="entry name" value="SLSG/EP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32444">
        <property type="entry name" value="BULB-TYPE LECTIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32444:SF132">
        <property type="entry name" value="S-LOCUS-SPECIFIC GLYCOPROTEIN_EP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01453">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002686">
        <property type="entry name" value="SLG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00108">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51110">
        <property type="entry name" value="alpha-D-mannose-specific plant lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50948">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041907145" description="Apple domain-containing protein" evidence="3">
        <location>
            <begin position="26"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="4">
        <location>
            <begin position="337"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7753550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="417" mass="47420" checksum="AF3899BB523BC65C" modified="2024-05-29" version="1">MAAYSYSLTLIFFCFLFRLFLISNAVVPANETFRYVNQGDLSFGNDYLTFVSEYAPTYRPLPPFTFPFQLCFYNTTPNAYTLGLRMGTLRSQSVMRWVWDANRGKPGVVGFAILSDGNIVLRDSNGNFVWQSFDYPTDTLLIGQSLRVRGGPYKLVSRESATNNVDGVYSFVIEPKRLALYYKNNMRYWSSTFPELNRDNVTIVNATLVVQETEYEENNFNALRCNLQNGQSFEFPTLDLGQVQYNSSLSYLRLGIDGNLRLYTYRANVRGNAVWTLLYTLFDKREDEGGMIFEDECQLPNRCGNFGLCEDSQCVGCPTPNGVFAWSKDCNTKSPGCNATGFKYYQLKGVDHFTVKYTAGTGPVKQSECESKCTKDCKCMGYFYHTDNSRCWIAYELKTLTRVGNSTSLAYIKIPVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GTF2</accession>
    <name>A0AAD5GTF2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_031140</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005811">
        <property type="protein sequence ID" value="KAI7751811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GTF2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000380">
        <property type="term" value="P:alternative mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034604">
        <property type="entry name" value="SRRP53"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31968">
        <property type="entry name" value="SERINE/ARGININE-RELATED PROTEIN 53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31968:SF4">
        <property type="entry name" value="SERINE_ARGININE-RELATED PROTEIN 53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="16"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="309"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="44"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="153"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="180"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="201"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="349"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7751811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="368" mass="42075" checksum="2CF275AC2CBA17B7" modified="2024-05-29" version="1">MEEEKAAAYYDELTRKGGGAARFKQGLGFSSSSSSTDNNPNDVVPPRASALPSSSSFLSSFVKASSPSKPSDVVSKQAQIESIQNKLKKKNDPSRESDKQSSRRRKSRSRSRSRSSRDDDERSRNRRRHRSRSRSRNRDDDERSRNRRRHRSSSRDRDRDGDRDRDRDRDRDRDRDRDRGRRRRSRSRNRSVSPRRRRSEKSRDEDERKSRKEKTGGVDYAKSIEGYDKMSPAERVKAKMKLQLSATAKHDEANGMGSGWERFNFDKDAPLDDEEIEAAEDDGALVKHIGQTFRFSAVEARREEKIKATHDEAIFGTSSLAPPVDTDSEADGNNSTRESPETAPVASLISDQVRSVQQGSWRDRIHKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BK49</accession>
    <name>A0AAD5BK49_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_004577</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012171">
        <property type="protein sequence ID" value="KAI7724700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BK49"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7724700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="66" mass="7663" checksum="B7C8C99F8690DFE5" modified="2024-05-29" version="1">MSLSMIPNESAYVMKGVQLLRQSRTSVKDQSEIWLKSKILKIIRTHLTHQGNHGSRYRGQPLMLRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BLM7</accession>
    <name>A0AAD5BLM7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_003315</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase A1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012189">
        <property type="protein sequence ID" value="KAI7724553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BLM7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000018">
        <property type="entry name" value="Aspartic proteinase-like protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032799">
        <property type="entry name" value="TAXi_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032861">
        <property type="entry name" value="TAXi_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13683">
        <property type="entry name" value="ASPARTYL PROTEASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13683:SF823">
        <property type="entry name" value="NEPENTHESIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14541">
        <property type="entry name" value="TAXi_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14543">
        <property type="entry name" value="TAXi_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041988662" description="Peptidase A1 domain-containing protein" evidence="3">
        <location>
            <begin position="24"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="4">
        <location>
            <begin position="85"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="103"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7724553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="427" mass="45937" checksum="00E24F24B9614280" modified="2024-05-29" version="1">MPVKTGSVLLLTVLLLLVTVCDGGGEFPATVLTLSRAFPANKRVELDVLKARDRVRHARILQGFTGGVVDFNVFGTSDPYYGGLYYTKVKLGSPPKEFNVQIDTGSDILWVTCSSCSDCPDTSGFGMPLNFFDAASSSTASMVSCSDRICSSNIKIADASCSDEFDQCSYEFRYADNSGTSAQGITPKVFSHCLLGDGTGGGKLVLGEIVAPNMVYSPIVPSQAHYNLELQSITVGEQLLSIDASAFATSDIQGTIVDTGTTLSYLTAQAYEPFVDAITAAVSQSATPISLKDMQCYLVTSSSSIATIFPEISLNFAGNAPMIVKPEKYLVQGDPIEGGVPWCIGFQKASNGLTVLGDIVLKDKIFVYDLSRNRMGWTDYDCSAEVNVSIKSYNNEYLDGHLSASISLRFTSLDVLLVHILLWSLLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-10-08" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BM81</accession>
    <name>A0AAD5BM81_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">M8C21_015243</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">AA19_3_7</strain>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010011791">
        <property type="protein sequence ID" value="KAI7725988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000083">
        <property type="entry name" value="Putative receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000035">
        <property type="entry name" value="Putative receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052232">
        <property type="entry name" value="RLK_Ser/Thr-Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47984">
        <property type="entry name" value="OS01G0323000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47984:SF14">
        <property type="entry name" value="OS01G0323000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="189"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="471"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="511"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAI7725988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="537" mass="59625" checksum="7CDEBAC6581437D3" modified="2024-05-29" version="1">MAGDLNNGLSKKVDGLGIEVWELIGIIVGLLIVGILCVLTCYLTSRKRVRGGRHAVPHSRIPTVSKEIKEVRVEQVSTANFAPQDGILLTIHDKNSEKDSDKVLVHLGVSKSRNGDNASSQGSFHHIDRDVCESGEDGSSGNFHLYKPSSSASHPISAPSPLVGLPEFSHLGWGHWFTLRDLELATGRFSKENVIGEGGYGVVYRGTLVNGNPVAVKKLLNNLGQAEKEFRVEVEAIGHVRHKNLVRLLGYCIEGTHRLLVYEYVNNGNLEQWLHGAMRQHGYLTWEARIKVLLGTAKALAYLHEAIEPKVVHRDIKSSNILIDDDFNAKVSDFGLAKLLGAGKSHITTRVMGTFGYVAPEYANTGLLNEKSDVYSFGVVLLESITGRDPVDYGRPAQEVNLVDWLKMMVGSRRSDEVVDPTIETKPSTRALKRALLTALRCVDPDSDKRPKMSXXXXLKRALLTALRCVDPDSDKRPKMSQVVRMLESEEYPLPREDRRRRSHASSVETESQRENYDTDKSDNPDPRSDSKRSLKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BNM7</accession>
    <name>A0AAD5BNM7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">ER membrane protein complex subunit 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_012946</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the EMC3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011610">
        <property type="protein sequence ID" value="KAI7726665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BNM7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072546">
        <property type="term" value="C:EMC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034975">
        <property type="term" value="P:protein folding in endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008568">
        <property type="entry name" value="EMC3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002809">
        <property type="entry name" value="EMC3/TMCO1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13116">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13116:SF5">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01956">
        <property type="entry name" value="EMC3_TMCO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF010045">
        <property type="entry name" value="DUF850_TM_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01415">
        <property type="entry name" value="DUF106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="14"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="119"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR010045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7726665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="247" mass="28083" checksum="ED1E45ED5B52DAEB" modified="2024-05-29" version="1">MAEELVLDTAIRDWVLIPLSVVMVLIGILRYFVSKLMRTSQVPDSKIIKEGQVIIRARNLRAAANFIPAKAFRARKVYYTNEENGLLHVPKGQAQNPQAQMFSDPNMAMDMMKKNLSMIIPQTLTFAWVNFFFSGFVAAKIPFPLTQRFRSMLQNGIDLSTVDVSYVSSRSWYFLNLFGLRGLFSLILGEENATDDTQRMMQMSGFGFDPSKSLGAEKDGLDIVQHDWVLPKFEQRAEAVLRKRLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BPC9</accession>
    <name>A0AAD5BPC9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ABC-type xenobiotic transporter</fullName>
            <ecNumber evidence="3">7.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">M8C21_007872</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">AA19_3_7</strain>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ATP + H2O + xenobioticSide 1 = ADP + phosphate + xenobioticSide 2.</text>
            <dbReference type="EC" id="7.6.2.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.2" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010011457">
        <property type="protein sequence ID" value="KAI7727040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BPC9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008559">
        <property type="term" value="F:ABC-type xenobiotic transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03244">
        <property type="entry name" value="ABCC_MRP_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000973">
        <property type="entry name" value="Multidrug resistance-associated protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000163">
        <property type="entry name" value="Multidrug resistance-associated protein member 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF430">
        <property type="entry name" value="ABC-TYPE XENOBIOTIC TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="252"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="288"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="379"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="13">
        <location>
            <begin position="3"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="14">
        <location>
            <begin position="255"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="13">
        <location>
            <begin position="400"/>
            <end position="632"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAI7727040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="649" mass="71853" checksum="DF4ED7C75D11CD85" modified="2024-05-29" version="1">MWAESCGLRVSVFGSIAYVSQTSWIQSGTIQDNILYGKPMDKTKYEKAIKACALDKDIEAFSHGDLTEIGQRGLNLSGGQKQRVQLARAVYNDADIYLLDDPFSAVDAHTAATLFNDCVMNCLEGKTVILVTHQVEFLSSVDNILVMQDGQIKQSGNYENILKAGTAFEELVNAHKEAITGLDSSSSSESKTMNKHQTLEENNNNYLEKENSEGHLLKGPPGVQLTEDEEKEIGNVGWKPFLDYVVISEGSWYLFLCVFTVSGFVALQAAASYWLAYGIQIPAVTTVMLISVYTLLSSVSTVFVFLRSIFAALLGLKASKMFFSKFTDAVFSAPMVFFDSTPVGRILTRASSDLSVIDFDIPFSFAFVIASSIELLAMILIMASVTWQVLIVAIFGILASKYTQLRYRPNAPLVLKGINCTFKEGTRVGIVGRTGSGKTTLITALFRLVEPDSGRILIDGLDICSIGLKDLRMNLSVIPQEPTLFRGSIRTNLDPLGLHSDDEIWKALEKCQLKTTIRNLPNLLDSSVSDEGENWSAGQRQLFCLGRVLLRRNKILILDEATASIDSATDVILQRIIREEFSSCTVITVAHRVPTVIDSDKVMVLSFGKMMEYDEPSKLMETDTFFSKLVAEYWSSCRRNSTQTLVDFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BPE9</accession>
    <name>A0AAD5BPE9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Arginyl-tRNA synthetase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_009685</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011764">
        <property type="protein sequence ID" value="KAI7726066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BPE9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004814">
        <property type="term" value="F:arginine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006420">
        <property type="term" value="P:arginyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009791">
        <property type="term" value="P:post-embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048608">
        <property type="term" value="P:reproductive structure development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001278">
        <property type="entry name" value="Arg-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035684">
        <property type="entry name" value="ArgRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11956:SF5">
        <property type="entry name" value="ARGININE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11956">
        <property type="entry name" value="ARGINYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00750">
        <property type="entry name" value="tRNA-synt_1d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01038">
        <property type="entry name" value="TRNASYNTHARG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="3 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 6" id="KW-0436">Ligase</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arginyl-tRNA synthetase catalytic core" evidence="7">
        <location>
            <begin position="85"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00033033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7726066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="530" mass="59491" checksum="940A1B2820EFA3BD" modified="2024-05-29" version="1" fragment="single">MAAAPELDNTNTAGSLQQQIAKLFEVSLKLTVPDHEPHVAPLIAVCSTKELAADYQCNNAMSLWSKIKRKGTEFRSPQAVGQRIQNMLTDGIDTWAPNLSVKRVIVDFSSPNIAKEMHVGHLRSTIIGDTIARMLEYSKVDVLRRNHVGDWGTQFGMLLEFLFEKFPDGEVNDHDIGGLEVFYKASKQRFDADPDFKERARKAVVALQSGKCEKYQKAWAQICEISRKGFQEVHQRLGIRLEEMGESFYKDYIPLALKELNDKGLIKDEDGTKVIEFVEGKKTLRLASTEKDGYNHASPDFAALWCRLHEEKVEWIIYVNLQGGTEEKFPNGWIQICEINGKGLKKVYQCHGVHLGESLCDTYIPLAIEEPIDKALIINDEGPRLIKIIEGKKAPPLIVTKNDGGYNYATTDLAALWYRLNVEKGEWIVYVTDVGQRDHFKKLFAAAKSAGWLPADESKYPKTSHVGFGLVLGDDGKRFRTRSTEVVKLVDLLDEAKARCKAALVERGKATEWTAEELEQTAEAVGYGAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BR44</accession>
    <name>A0AAD5BR44_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Pseudouridine synthase I TruA alpha/beta domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_032976</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a uridine in mRNA = a pseudouridine in mRNA</text>
            <dbReference type="Rhea" id="RHEA:56644"/>
            <dbReference type="Rhea" id="RHEA-COMP:14658"/>
            <dbReference type="Rhea" id="RHEA-COMP:14659"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a uridine in tRNA = a pseudouridine in tRNA</text>
            <dbReference type="Rhea" id="RHEA:54572"/>
            <dbReference type="Rhea" id="RHEA-COMP:13339"/>
            <dbReference type="Rhea" id="RHEA-COMP:13934"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the tRNA pseudouridine synthase TruA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011273">
        <property type="protein sequence ID" value="KAI7728101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BR44"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990481">
        <property type="term" value="P:mRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02568">
        <property type="entry name" value="PseudoU_synth_PUS1_PUS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.580:FF:000002">
        <property type="entry name" value="tRNA pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.660:FF:000002">
        <property type="entry name" value="tRNA pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.660">
        <property type="entry name" value="Pseudouridine synthase I, catalytic domain, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.580">
        <property type="entry name" value="Pseudouridine synthase I, catalytic domain, N-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001406">
        <property type="entry name" value="PsdUridine_synth_TruA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020097">
        <property type="entry name" value="PsdUridine_synth_TruA_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020095">
        <property type="entry name" value="PsdUridine_synth_TruA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041708">
        <property type="entry name" value="PUS1/PUS2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020094">
        <property type="entry name" value="TruA/RsuA/RluB/E/F_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00071">
        <property type="entry name" value="hisT_truA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11142">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11142:SF4">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01416">
        <property type="entry name" value="PseudoU_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Pseudouridine synthase I TruA alpha/beta" evidence="11">
        <location>
            <begin position="220"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="9">
        <location>
            <position position="82"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00036943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR641708-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR641708-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7728101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="829" mass="93114" checksum="820182CB17407060" modified="2024-05-29" version="1">MENQTPSFSDHNAIDNNPQHKQQKVAIIFAYCGAGYQGMQANPGAKTIEFELQQALFLSGSIPKPDRHCPKRFHFARAARTDKGVSAIGQVVSGTFYVDPHGFVERINNNLPGKIRVFGFKDVTTSFSAKKCCVGRKYVYLLPVFALDCCSHRDRESVLASLGSGNQLVRCLECSERGRKVFRGSGISSNTLSALENGNGYNGDVFSYGEKERVRFNRILKKYEGTHNFHNFTTKTKATDPAANRCIVSFNADTTVMIDGLEFVKCEVVGQSFMLHQIRKMIGLAVAVMRNCAPESLIETALQPRKWFPASTECLLENQAELGDVRMCAFLIDHCNLRKRYVKINVPTAPEVGLYLEECFFSSYNQKYKDTHEEVSMKDYAEEAEEFKLEHIYPHIASTEHKEGTVAVWLHSLNYRNYPDLCPEENRVANEVSGTQTEETTRINMETLDYRKHPDLGPEEKSGANEVARIRTEETTRCNQDALNYPNHVSPSCFIRRPSRHYEKPIVHWIDLEHLATTMKNRLNLVLPYIHELSVRYGGETDTIIADVNMMVDTAMSVTYNRLRFILESYAPGVCVAQMNPKSPFSNKFKFPAFISSVLGSIGPLRIMDGLQDTMVVYATSADRSKTYGRAQHFEFISGHFYRLIDALEAIGVPLAPIDVKGSNRAGSFYPTILLGRDNGWLEFTATCHYSNYRHQDIDSAWLTLNDDPALRPFPDCSLRIECCTDPDVVKAVIASRPEGVPDDVDGATALARPIGLNFNVDAFGVRPLVAAEGGNPTVPAAVYIVGRGNTRYGCTSLARGLTEYDMTWRLREYIMRYDSMCRTASKGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BSD7</accession>
    <name>A0AAD5BSD7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Small EDRK-rich factor-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_019332</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011345">
        <property type="protein sequence ID" value="KAI7727583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BSD7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1050.10">
        <property type="entry name" value="At2g23090-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045230">
        <property type="entry name" value="MBS1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026939">
        <property type="entry name" value="ZNF706/At2g23090_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21213">
        <property type="entry name" value="GEO09665P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21213:SF0">
        <property type="entry name" value="ZINC FINGER PROTEIN 706"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118359">
        <property type="entry name" value="Expressed protein At2g23090/F21P24.15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="64"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7727583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="83" mass="9500" checksum="F0590934681B02A8" modified="2024-05-29" version="1" fragment="single">REKQREKMTRGKQKIEAQRKNAEKNSKSKGSQLEHRAVALKVQLANHINSMGWDLQVQLANQNQLGDHYGSKHPKEKPPSNSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BT78</accession>
    <name>A0AAD5BT78_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">1,3-beta-glucan synthase</fullName>
            <ecNumber evidence="3">2.4.1.34</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">1,3-beta-glucan synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">M8C21_025870</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">AA19_3_7</strain>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>[(1-&gt;3)-beta-D-glucosyl](n) + UDP-alpha-D-glucose = [(1-&gt;3)-beta-D-glucosyl](n+1) + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21476"/>
            <dbReference type="Rhea" id="RHEA-COMP:11146"/>
            <dbReference type="Rhea" id="RHEA-COMP:14303"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37671"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.4.1.34"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 48 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.34" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010011047">
        <property type="protein sequence ID" value="KAI7729196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000148">
        <property type="term" value="C:1,3-beta-D-glucan synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003843">
        <property type="term" value="F:1,3-beta-D-glucan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006075">
        <property type="term" value="P:(1-&gt;3)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.270">
        <property type="entry name" value="Vacuolar protein sorting-associated protein vta1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058851">
        <property type="entry name" value="CALS1_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026899">
        <property type="entry name" value="FKS1-like_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003440">
        <property type="entry name" value="Glyco_trans_48_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023175">
        <property type="entry name" value="Vta1/CALS_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12741:SF47">
        <property type="entry name" value="CALLOSE SYNTHASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12741">
        <property type="entry name" value="LYST-INTERACTING PROTEIN LIP5 DOPAMINE RESPONSIVE PROTEIN DRG-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25968">
        <property type="entry name" value="CALS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14288">
        <property type="entry name" value="FKS1_dom1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02364">
        <property type="entry name" value="Glucan_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01205">
        <property type="entry name" value="FKS1_dom1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0133">Cell shape</keyword>
    <keyword evidence="11" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="500"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="531"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="569"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="600"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="667"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="734"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1480"/>
            <end position="1502"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1537"/>
            <end position="1555"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1561"/>
            <end position="1582"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1626"/>
            <end position="1642"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1648"/>
            <end position="1672"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1725"/>
            <end position="1742"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1754"/>
            <end position="1774"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1786"/>
            <end position="1808"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1814"/>
            <end position="1836"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1857"/>
            <end position="1878"/>
        </location>
    </feature>
    <feature type="domain" description="1,3-beta-glucan synthase component FKS1-like" evidence="15">
        <location>
            <begin position="344"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM01205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAI7729196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="1911" mass="219159" checksum="939BC2E1DB663062" modified="2024-05-29" version="1">MSNVGPLWERLVRAALRRERTGIDAYGRPAGGIAGNVPSSLSNSRDIDPILRAADEIQDEDPNISRILCEHAYTLAQNLDPNSEGRGVLQFKTGLMSVIKQKLAKREGADFDRSQDITRLREFYKQYREKNNVDKLREEEMELRESGTFSGNFQELERKTLKRKKVFATLKVLGTVLEQLSKEVSPEEAEGLIPEELKRVMESDAAMTEDLIAYNIIPLDAPALTNAITSFPEVRAAIASLKYFRGLPQLPGNFPIPATRSADVLDFLHYTFGFQKGNVSNQRENIVNLLSNEQSRLGTPDEHEPKLDETAVENVFLKSLDNYIKWCNYLGIPLMWSNMDVSKENKLLVISLYFLIWGEAANVRFLPECLCYIFHRMGEELNDHILRQPLAKPAASCVTEDGVSFLEQVIVPLYNVIAAEANNNENGKAPHSAWRNYDDFNEYFWSRSCLKLGWPLDNNSSFLLRPSPRSKNILKTNVTKRRGKTSFVEHRTFFHLYHSFHRLWIFLFMMFQALAIIAFNDGRVDSRTIRLLLSLGPTFFVMKLFQSALDVLIMYGAYSTTRLLAVSRIFVRFIWFSFVTIFICFLYVKGLQDTTNSISGSIVFKIYIIVLGIYGGIQFFWSSLARLPSCHHLFNRCDNSSVVRLVKWIHQEHYYVGRGMYEATTDYIMYMLFWLVVLGGKFAFAYFLQIKPLVEPTRTIIDIKDVRYSWHDFVSKNNHNALTIASLWAPVFSIYLLDVHIFYTIISAVVGFLLGARDRLGEIRSLDAVHKLFERFPEAFMNNLHIPLSKSLPLPFPLLKVQEKNKFDAARFSPFWNEIVNNLREEDYITNLEMELLQMPKNTDSVPMVQWPLFLLASKIFVAKDMAAESDSQEELWDRISRDDYMKYAVEECFYTIKLILTSIFDKDGQEWVNRIYEDIRGSIVKGTIHSDFQLKKLSLVIQKVTALTGILKEVGTPELETGARNAALDLYDVIQLDFFSVNMRENYATWSMLIKARKEGRLFSKLKWPRDPELRLQIRRLHSLFTIKDSAVNVPRNLEARRRLEFFTNSLFMDMPRTKPVREMLSFSVFTPYYSETVLYSIDELLKKNEDGISVLFYLQKIYPDEWKNFLARIGRDENTHESVLIDNEDDNLELRFWASYRGQTLSRTVRGMMYYRKALMLQAYLERMTVGDIEAAVPANEATDTKGFEFSPEARAHADLKFTYVVTCQIYGKQKEEQKPEAADIALLMQRNEALRVAFIDEVETLSGGEIKKSYFSKLVKGDINGKDKEVYSIKLPGNPKLGEGKPENQNHAIVFTRGNAVQTIDMNQDNYFEEALKMRNLLEEFHAYHGIRPATILGVREHVFTGSVSSLASFMSNQETSFVTLGQRVLASPLKVRMHYGHPDVFDRVFHITRGGISKASRVINISEDIFAGFNSTLRQGNITHHEYIQVGKGRDVGLNQIALFEGKVAGGNGEQVLSRDIYRLGQLFDFFRMLSFYFTTVGFYFCTMLTVITVWLFLYGKLYLALSGVGEDIQNRSSVLDNKALTAALNTQFLFQIGVFTAIPMVLGFILEQGFLRAVVSFITMQFQLCTVFFTFSLGTRTHYFGRTILHGGAKYHATGRGFVVRHIRFAENYRLYSRSHFVKGLEVVLLLVVYLSYGYNDGIVGYVLLSVSSWFMALSWLFAPYLFNPSGFEWKKTVEDFRDWTNWLFYRGGIGVKGEESWEAWWDEEMSHIRNFGGRVMETILSLRFFIFQYGVIYKLDVQGSNTSLSVYGLSWLVLLALILLFKIFTFSQKISVNFQLILRFIQGVAFLLAIAGIAVAIVLTDLSITDIFAAILAFIPTGWGILSICVAWKPVVKKTGLWKSVRSLARLYDAGMGMLIFVPIAFCSWFPFISTFQTRLMFNQAFSRGLEISLILAGNNPNTGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BTZ7</accession>
    <name>A0AAD5BTZ7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_010273</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011006">
        <property type="protein sequence ID" value="KAI7729435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BTZ7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000385">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45717:SF8">
        <property type="entry name" value="OS01G0301000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45717">
        <property type="entry name" value="OS12G0527900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="6">
        <location>
            <begin position="143"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7729435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="507" mass="58462" checksum="2DDB937E43B94461" modified="2024-05-29" version="1">MMIIRTRIRIPIPGTSTMVKTFSTVMEQSNVSSEVVVSPTHTTEKRLYRRLSELGATGGTVAQTLNHYMREGNFVKKIELERCVRELRRYGKYHHALEMEWMDKRDINFSNTDLAVRLDLISKVHGVLAAEEYFNNLSPQFHTRNTYGALLNTYCKLKMADKAFALFNIIDSKNFTTSLAYNNIMTLYIGLEQPEKVPPLIDEMKIRKIHLSTFTYNIWMQSFSLLGDIEGVERVFEEITRDQKETCNWTTYSNMAGAYVKAGRVEKAESALKMLENEIKHPTREAYHFLITFYSTLNNKEQVHRVSSLLKSSFETTSNMSYRIILQALSKVNDLEGMKKWFEEWETNCSYYDTRVANVVISGYLRHNMVEEAELVFHGSVKRSSGPFVQTLVLFMKHYLARQQIDLAVKCLETAAAQAEICNWYPHSESIDVFLEYFEKEKDVVGAEKLYAILQKLNCVDDKVYSHLLQTYIAAGKTEPDMGRRIERDGIVVTPELGNMLKKVCSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BV59</accession>
    <name>A0AAD5BV59_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_025638</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010903">
        <property type="protein sequence ID" value="KAI7729854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BV59"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7729854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="76" mass="8632" checksum="CAC60B1052477D2D" modified="2024-05-29" version="1">MISGFLCQWLRAVGFRTQRYNRQKAILLSKDQCCQHVRQSILVDAVTKPNKRPVVRRFRASSESSLGDYGESLSGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BV64</accession>
    <name>A0AAD5BV64_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_023068</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010821">
        <property type="protein sequence ID" value="KAI7730231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BV64"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="171"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="211"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="292"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7730231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="316" mass="35578" checksum="18D3798D7F8DAA88" modified="2024-05-29" version="1">MLFFEFKPYFDKMVFFVCTRGYGLLLDAASGEARAVLISVDQLFCKWETLTSLAVKVCAMGRFSFKFYDWCVLAREKMSPLKKKLVQWFGHFASICFGRSGLEKDCCNPVVADPSRGSRLEKLRPKHCFRMDNKFLLPSCLPVSGKGLLLDAASGEVSGELHDYHFSASGLALVAWVVAVTAHGSGVVIVLSRLYDKLHAAGYHWGSCTSVFMFAIPHYGCLRLLGLWFCLQLDDSQDLLQRVTFFTKCQKNKFSMGMSCMDDRLRFYGLRERISYKEEAVFLFPAIRPNTFTSLGIDAIIGLLALNTAYLALASW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BVB1</accession>
    <name>A0AAD5BVB1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Autophagy-related protein 9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_001420</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Phospholipid scramblase involved in autophagy. Cycles between the preautophagosomal structure/phagophore assembly site (PAS) and the cytoplasmic vesicle pool and supplies membrane for the growing autophagosome. Lipid scramblase activity plays a key role in preautophagosomal structure/phagophore assembly by distributing the phospholipids that arrive through ATG2 from the cytoplasmic to the luminal leaflet of the bilayer, thereby driving autophagosomal membrane expansion.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Preautophagosomal structure membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the ATG9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010835">
        <property type="protein sequence ID" value="KAI7730132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BVB1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034045">
        <property type="term" value="C:phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034497">
        <property type="term" value="P:protein localization to phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061709">
        <property type="term" value="P:reticulophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007241">
        <property type="entry name" value="Autophagy-rel_prot_9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13038">
        <property type="entry name" value="APG9 AUTOPHAGY 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13038:SF10">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04109">
        <property type="entry name" value="ATG9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0072">Autophagy</keyword>
    <keyword evidence="8 10" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="93"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="158"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="321"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="404"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="440"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU364027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7730132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="755" mass="86993" checksum="7285A0B18C8374D1" modified="2024-05-29" version="1">MMFGGQKRGTILDNIKQKVLGGSSLSTRLLSDVAHEIELSDYQRAPSHGSESPSGLLDDEHLKEEPIADLDLFFERIYSYYCGKGLQCIIIKWIVELLTLAFIILFSGFFLLFFNWNGIRNVKCGMDAVESGKKPCDLAKEVLHQHPLTPLTLLKVVILGYLGITSSYWIFCFLRFFAQLKDTLEIRKFYINSLNVKDSEIHTMPWSTILEKVVKVQESRQLCVVKDLSSHDIIMRMMRKENYLIGMLNKGVLAFPISHWAPGAGPTSKKSPNGTQNRLMLTKTLEWTLNWCILQSMFDRNFCIRRDFVTNPKTLKKTLTVVGLAMLLLSPFLVVFMLVYLFLRHAEQFYKDPSTASSRRWSNLSKWVLREFNEVDHLFKHRINRSVAPASDYLKQFPSPILSIIAKFISFVSGGFTAVLIIIAFLEESLLEGHIFGRNLLWYGAVFGAITAISRAAVSDELLVVDPHGTMSLICKDTHFMPKRWRAKENTEFARMEFETLFQYTGKINHGNSKYASPHNSSRILRSSQGKMEKSFLSSFHGSYPMWEPNVDGKQFLSTLKKFQQQRSPNQMQKWLFNLDNKDHPYILDWYYTSHHHNHHRPRDIETGLIDVEDEAITNTWVPPDLGSNQDQLDDNWHTSFEDRINSNLEASTSGTIIQHLDLRNASQATRSHWWARKGGSPMARTGVGPETSFLEPPNFYQNSYYDHYDNSSDKSFREQDPRFSWGIKENDFNLLFDDIFDAHSQSPPRTFFAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BVB2</accession>
    <name>A0AAD5BVB2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_033316</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010842">
        <property type="protein sequence ID" value="KAI7730110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BVB2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010903">
        <property type="entry name" value="DUF1517"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053023">
        <property type="entry name" value="FLAP_modulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33975">
        <property type="entry name" value="MYELIN-ASSOCIATED OLIGODENDROCYTE BASIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33975:SF2">
        <property type="entry name" value="MYELIN-ASSOCIATED OLIGODENDROCYTE BASIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07466">
        <property type="entry name" value="DUF1517"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037221">
        <property type="entry name" value="DUF1517"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="43"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7730110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="297" mass="33142" checksum="828D88A5FAE9AC4F" modified="2024-05-29" version="1">MSMNNALQNPIITVMVLLSLLMVMYNPDHHLVLAASGGRMGGTSFRSSSSSSSRRTSTSSYSTSYSIPRFSYSRRFSTRTGGSPQSRSSKSLSTQISSPGQHVRYADEDHVETSVLKLQVGLLVTARSLQKDLNKIAETADTSCPKGFRYILKETTLSMLQHSDYCISAYSSVDVKRGEEECEKQFNKLSKEEKDKFDEETLVNFNNIRKQTATCHETVSEGLCNKYIVVTIIVAASGAHELPPIKTRELLVTAFQKLASFPSNKILAAEVLWTPQKEDDSLTEHRMLQDYPQLHPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BVF7</accession>
    <name>A0AAD5BVF7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_029875</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010862">
        <property type="protein sequence ID" value="KAI7729989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BVF7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7729989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="182" mass="20529" checksum="FD73DDBB5AECFBF9" modified="2024-05-29" version="1" fragment="single">RERERKGERFPPTATGCATESWKHCDSDVFLARTQCSLKDGEMGCPLMVLTGGGVRVRVLHVLMLERVNGGRMNLNSDMALSIMIFLNKLSYTPLSAGLPTETTNESVGGRYNIAMAVDINPKLLTRIMCSNYQSPNSNNWVFGTIAGNLYEELKSLIWEFEYDQMKDDTFTQFLSAWSAYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BW21</accession>
    <name>A0AAD5BW21_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">VTT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_011539</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010680">
        <property type="protein sequence ID" value="KAI7730697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BW21"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032816">
        <property type="entry name" value="VTT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46431">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46431:SF5">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09335">
        <property type="entry name" value="VTT_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="130"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="215"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="258"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="VTT" evidence="2">
        <location>
            <begin position="118"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7730697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="317" mass="35949" checksum="381B9767C83D7E85" modified="2024-05-29" version="1">MPSQEDAAKHLTGTRGLAREDSEHVRLVISSNERRVVEADGLQSQTERISESLKWWLKAVTWSIVAIIVTLIFLKWGVPFVFEKVLLPVLQWEATAFGRPALALILVGSLAFFPVVLIPSGPSMWLAGMIFGYGLGFVIIMAGTTVGMILPYFIGLLFRQRIHKWLKKWPQTAAMIRLAGEGDAFQQFRVVTLFRISPFPYTIFNYAIVVTNMRFWPYLCGSIAGMIPEAFIYIYSGRLIRTFADVQYRKHRMTTLEIIYNVISFIIAGAMTIGFTIYAKKALKELEDEDKETDDVLDSGNIQLQKLPLETHNNFSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BWP3</accession>
    <name>A0AAD5BWP3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Malonyl-CoA:ACP transacylase (MAT) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_021399</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010626">
        <property type="protein sequence ID" value="KAI7730800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BWP3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.250">
        <property type="entry name" value="Malonyl-CoA ACP transacylase, ACP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.366.10">
        <property type="entry name" value="Malonyl-Coenzyme A Acyl Carrier Protein, domain 2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001227">
        <property type="entry name" value="Ac_transferase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014043">
        <property type="entry name" value="Acyl_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052760">
        <property type="entry name" value="Mitochondrial_malonyltrans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47170">
        <property type="entry name" value="MALONYL-COA ACP TRANSACYLASE, ACP-BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47170:SF2">
        <property type="entry name" value="MALONYL-COA:ACP TRANSACYLASE (MAT) DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00698">
        <property type="entry name" value="Acyl_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00827">
        <property type="entry name" value="PKS_AT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Malonyl-CoA:ACP transacylase (MAT)" evidence="1">
        <location>
            <begin position="104"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7730800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="351" mass="37709" checksum="56850229CFB7ECA2" modified="2024-05-29" version="1">MFLSNSPHFLRPFSPFNLLSTTTMASSSSSSLLLPSLSLNHHHPSLHSSSFKNPSSVSFRTLNHHGFHRLNLVKSRVSMSLSTGPTTVIDDALFIDYKPTTAFLFPGQGAQAVGMGQEALKVPAAAELYNKANEILGFDILDVCINGPKEKLDSTIIDSVDVTCGLSLGEYTALAFAGAFSFEDGLKLVKLRGQAMQEAADAAKGAMVSIIGLDSDKVQQLCDAANEEVEEAEKVQIANFLCNVRLAVAGAFHTRFMNPALSQLESALSATEIKTPRIPVISNVDAQPHADPETIKKILARQVTSPVQWETTVKTLLSRGLVKSYELGPGKVIAGIVKRMDKTSPVENVSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BWW3</accession>
    <name>A0AAD5BWW3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">25S rRNA (uridine-N(3))-methyltransferase BMT5-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_014065</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010888">
        <property type="protein sequence ID" value="KAI7729906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BWW3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070042">
        <property type="term" value="F:rRNA (uridine-N3-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019446">
        <property type="entry name" value="BMT5-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11538:SF26">
        <property type="entry name" value="FERREDOXIN-FOLD ANTICODON-BINDING DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11538">
        <property type="entry name" value="PHENYLALANYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10354">
        <property type="entry name" value="BMT5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="25S rRNA (uridine-N(3))-methyltransferase BMT5-like" evidence="1">
        <location>
            <begin position="26"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7729906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="126" mass="14137" checksum="8CCE5D59B165BFDF" modified="2024-05-29" version="1">MSMIIGMNRVEEAKWAKHYSSDHEILLVGEGDFSFALSLATAFASASASNIVATSLDSYEVVIKKYLRARTNLDSLYNAGAKLLFGVDAMTMKLHPHLHWRKFDRIIFNFPHAGFSGKEDDQLVIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BWZ3</accession>
    <name>A0AAD5BWZ3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Glucose-6-phosphate 1-dehydrogenase</fullName>
            <ecNumber evidence="10">1.1.1.49</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_017499</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>D-glucose 6-phosphate + NADP(+) = 6-phospho-D-glucono-1,5-lactone + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15841"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57955"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:61548"/>
            <dbReference type="EC" id="1.1.1.49"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the glucose-6-phosphate dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.49" evidence="10"/>
    <dbReference type="EMBL" id="JAMZMK010010659">
        <property type="protein sequence ID" value="KAI7730754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BWZ3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004345">
        <property type="term" value="F:glucose-6-phosphate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009051">
        <property type="term" value="P:pentose-phosphate shunt, oxidative branch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000013">
        <property type="entry name" value="Glucose-6-phosphate 1-dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00966">
        <property type="entry name" value="G6PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001282">
        <property type="entry name" value="G6P_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019796">
        <property type="entry name" value="G6P_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022675">
        <property type="entry name" value="G6P_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022674">
        <property type="entry name" value="G6P_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00871">
        <property type="entry name" value="zwf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23429:SF0">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE 1-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23429">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE 1-DEHYDROGENASE G6PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02781">
        <property type="entry name" value="G6PD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00479">
        <property type="entry name" value="G6PD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000110">
        <property type="entry name" value="G6PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00079">
        <property type="entry name" value="G6PDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00069">
        <property type="entry name" value="G6P_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 10" id="KW-0313">Glucose metabolism</keyword>
    <keyword evidence="6 10" id="KW-0521">NADP</keyword>
    <keyword evidence="7 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucose-6-phosphate dehydrogenase NAD-binding" evidence="11">
        <location>
            <begin position="33"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="Glucose-6-phosphate dehydrogenase C-terminal" evidence="12">
        <location>
            <begin position="223"/>
            <end position="502"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7730754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="515" mass="59103" checksum="C1DAABDAB91B42F5" modified="2024-05-29" version="1">MGTGQWTVEHRAPIRSDSLLNEYVPEIGCLSIIVLGASGDLAKKKTFPALFHLYRQGFIQSQDVHIFGYARTAISDDDLRTRIRGYLTPCKGFEQTHEEDVAQFLQLIKYVSGAYDTEEGFRLLDKAISEYELSKNSTEGSSRRLFYLALPPSVYPPVCKMIRSYCMNKSDLGGWTRIVVEKPFGRDLASAEELSAQIGELFDEPQIYRIDHYLGKELVQNLMVLRFANRFFLPLWNRDNISNIQIVFREDFGTDGRGGYFDQYGIIRDIIQNHLLQVLCLVAMEKPVSLKPEHVRDEKVKVLQSVLPIEDNEVVLGQYEGYTDDPTVPDNSNTPTFATMVLRIHNERWEGVPFIMKAGKALNSRKAEIRVQFKDVPGDIYKCQKQGRNEFVIRLQPSEAMYMKLTVKQPGLEMTTAQSELDLSYGQRYQETIIPEAYERLILDTIRGDQQHFVRRDELKAAWEIFTPLLHRIDRGEMKPLPYKPGSRGPEEADKLAQKAGYVQTHGYIWIPPTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BY82</accession>
    <name>A0AAD5BY82_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_015093</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010678">
        <property type="protein sequence ID" value="KAI7730703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BY82"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050052">
        <property type="entry name" value="ATP-dep_Clp_protease_ClpX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48102:SF7">
        <property type="entry name" value="ATP-DEPENDENT CLP PROTEASE ATP-BINDING SUBUNIT CLPX-LIKE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48102">
        <property type="entry name" value="ATP-DEPENDENT CLP PROTEASE ATP-BINDING SUBUNIT CLPX-LIKE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07724">
        <property type="entry name" value="AAA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="2">
        <location>
            <begin position="165"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="46"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7730703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="290" mass="32064" checksum="1F6B3711C3215A5D" modified="2024-05-29" version="1" fragment="single">FYPPFLKLNDTINNNINNNNNNIIINIHRPYILRRYKSDGGGGGFDNNNNGKTTSSSSMTRRIKTEANCPRCCKPMDLIFSNNRHVIPPGNDGYKDEMETTPYEGVTDAADRKIGGPTRWVRYKVLSVAVYNHYKRIHHASLPNRSEGETGGASIEDDDDNVELEKSNVLLMGPTGSGKTLLAKTLARFVNVPFVIADATTLTQAGYVGEDVESILHKLLTVAEFNVQAAQQGMVYIDEVDKIIKKSESMNISRDVSGEGVQQALLKMLEGTIVNVPEKGARKHPRGENI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-10-08" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BYK2</accession>
    <name>A0AAD5BYK2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_020881</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010655">
        <property type="protein sequence ID" value="KAI7730776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010333">
        <property type="term" value="F:terpene synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016102">
        <property type="term" value="P:diterpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046246">
        <property type="term" value="P:terpene biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00684">
        <property type="entry name" value="Terpene_cyclase_plant_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.130:FF:000001">
        <property type="entry name" value="Isoprene synthase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.130">
        <property type="entry name" value="Terpene synthase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044814">
        <property type="entry name" value="Terpene_cyclase_plant_C1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001906">
        <property type="entry name" value="Terpene_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036965">
        <property type="entry name" value="Terpene_synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050148">
        <property type="entry name" value="Terpene_synthase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005630">
        <property type="entry name" value="Terpene_synthase_metal-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31225">
        <property type="entry name" value="OS04G0344100 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31225:SF196">
        <property type="entry name" value="TERPENOID CYCLASES_PROTEIN PRENYLTRANSFERASE ALPHA-ALPHA TOROID-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01397">
        <property type="entry name" value="Terpene_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03936">
        <property type="entry name" value="Terpene_synth_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Terpene synthase N-terminal" evidence="5">
        <location>
            <begin position="19"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="Terpene synthase metal-binding" evidence="6">
        <location>
            <begin position="257"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="Terpene synthase metal-binding" evidence="6">
        <location>
            <begin position="398"/>
            <end position="521"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7730776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="578" mass="67269" checksum="D85A9CA7AC213DA4" modified="2024-05-29" version="1">MSIKQEEVVRPNANFHPGLWGDQFLVYEEHADKQAEVEQRVNDLKEVVRKDMLSSLEVQTEHANLLKLIDVIQRLGIAYLFEEEIEQAMQHIYDKYGDDWKGGNPSLWFRILRQEGFHVSCDVFKQYKDENGSFKESLTSDVEGLLELYEATYLKVEGEVVLEDALVFTRTHLEKIANNLTQTNSVLSTHIHDALKLPLFKRVPRLEALSYIPFYEQQDCHNESLLKLAKLAFNLVRSLHKKELSDMSRWWKKLVPTSLPYVRDRLVESYFWALCVYFEPKYSRARIFLTKVFAMTTILDDTYDAYGTYEELKIFTEAIERWSITSIDELPEYMKQIYQEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKGCRIQMKDLARSYMTETYWAHKGIVPTPEDHIAVSYVSSGYGMLIATCFVGMGDMVTDDSFKWALSKPPIVKASCVMARLMNDFYSQKEEKDRMHVASNVVSYMKQYDVTKEHAHKVYTDKIEDLWNEITHEFIMNRQVPLPLIYCSINLTRSLAIIYERNDGFTQVTKELVDHIKSFLVHGVNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C1M6</accession>
    <name>A0AAD5C1M6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_008181</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009932">
        <property type="protein sequence ID" value="KAI7733666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C1M6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7733666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="16" mass="1868" checksum="C9687F2D9F39D462" modified="2024-05-29" version="1">MLFSKYASHNICGRLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C2E8</accession>
    <name>A0AAD5C2E8_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_028637</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009897">
        <property type="protein sequence ID" value="KAI7733852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C2E8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7733852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="77" mass="8305" checksum="301ACFBB90617706" modified="2024-05-29" version="1">MFCPVVIDQGLSHGHCYGYGNPAMAVRSMVSLLSGGNGGSACATDSYRVYLHYINPAITVYREGAVKADCRDDLQFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C772</accession>
    <name>A0AAD5C772_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_022095</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009499">
        <property type="protein sequence ID" value="KAI7735349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C772"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000554">
        <property type="entry name" value="protein indeterminate-domain 12-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000131">
        <property type="entry name" value="protein indeterminate-domain 5, chloroplastic-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055187">
        <property type="entry name" value="C2CH-3rd_BIRD-IDD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055185">
        <property type="entry name" value="C2CH-4th_BIRD-IDD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055186">
        <property type="entry name" value="C2H2-2nd_BIRD-IDD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031140">
        <property type="entry name" value="IDD1-16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10593">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE RIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10593:SF150">
        <property type="entry name" value="ZINC FINGER, C2H2-LIKE, ZINC FINGER, DOUBLE-STRANDED RNA BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22995">
        <property type="entry name" value="C2CH-3rd_BIRD-IDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22992">
        <property type="entry name" value="C2CH-4th_BIRD-IDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22996">
        <property type="entry name" value="C2H2-2nd_BIRD-IDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="92"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="17"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="442"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="501"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="17"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="448"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="504"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7735349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="524" mass="58109" checksum="0E435A59A1E10C4D" modified="2024-05-29" version="1" fragment="single">MREMKVMFLDDSMSNLTSASNEASLSSSSNKNEVGTMYPPSQQVQQSFASVPTNSNNQTHTNKKKRNLPGNPDPEAEVIAMSPKTLMATNRFVCEICNKGFQRDQNLQLHRRGHNLPWKLKQRSKQEVVRKKVYVCPEPSCVHHEPARALGDLTGIKKHYSRKHGEKKWKCDKCSKRYAVQSDWKAHSKICGTREYRCDCGTLFSRRDSFITHRAFCGALAGETARSSSSTLLSPNLPLPLHLPLNFPLKTEPHLFQYPQLNPNIMTNMTNDLQQNNQLPSWFVHHHHQPNSNLNNASNNNINNNNIDFPSPSPSHMSATALLQKAAQMCETMSKPAGSTNNVIVMSQQQQQQQHQSILSGSHQNDHMCAPTSLLTAHHHASGLETLSSIDENQQEMLGLSGGVFTNNAQPNSLACESVGSINAFQEAYHNAFQQPFYPKPATSNPIGGSGGGGSRSGGVGVRGGNNELTRDFLGLTGFPPPNEDQHFLSVAGLDQLNTSSPYHQLQQHDPNQNPNQNQNQLPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C7D9</accession>
    <name>A0AAD5C7D9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">ABC transporter B family member 28</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_030564</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009417">
        <property type="protein sequence ID" value="KAI7735908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C7D9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015421">
        <property type="term" value="F:ABC-type oligopeptide transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090374">
        <property type="term" value="P:oligopeptide export from mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000604">
        <property type="entry name" value="ABC transporter B family member 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394:SF7">
        <property type="entry name" value="ABC TRANSPORTER B FAMILY MEMBER 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394">
        <property type="entry name" value="ATP-DEPENDENT PERMEASE MDL1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="115"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="160"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="225"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="10">
        <location>
            <begin position="116"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="438"/>
            <end position="667"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7735908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="673" mass="72954" checksum="1605060F16082F3B" modified="2024-05-29" version="1">MASLPLFLPLHPSTTTTNHHHCHRPLITHRRPLLSPTYQQQQHHLRPTNIHNKPNFTTTSAYITGPASDAIVSSDDPKVDDVVSGSVPLPLPVQQFDAISLGLLFRLLSRHKLRIAASVASLVCCTTCTLSMPILSGRFFEVLIGARAEPLWQLLSKVAVLYTLEPIFTVIFVVNMNTIWEKVMSSLRAQIFGSVLIHKVDFFDKYKVGELTALLTSDLGSFKNIVIGTMCLLFILAPQLAPILGVLMLAVSVLVAVYKRSTVKVFKAYGLTQASIADCVTETFSAIRTVRSFGGEKRQMLTFGNQVLEYQKSGIQLGVYKSINESITRVAVYVSLLALYILGGNKVKAVQGLVNTIGDLRGAFAATERINSILSGSEIDQALAYGLQKDIKQGSGTYNNLKMFFNDQNDKSRSKNTRYMSSLTAASSVRSLASSGDICLEDVHFAYPLRPDVEVLNGLDLTLKCGTVTALVGSSGAGKSTVPTQGRITVAGEDLRTFDKSEWARIVSIVNQEPVLFSVSVGENIAYGLPDEHVSKDDVIAAAKAANAHDFIISLPEGYDTLVGERGGLLSGGQRQRVAIARALLKNAPILILDEATSALDAVSERLVQDALNRLMKGRTTLVIAHRLSTVQNADQIAVCSEGKIAELGTHAELLAKKGDYASLVGSQRLAFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C7S2</accession>
    <name>A0AAD5C7S2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein MULTIPLE CHLOROPLAST DIVISION SITE 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_003465</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009320">
        <property type="protein sequence ID" value="KAI7736295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C7S2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009706">
        <property type="term" value="C:chloroplast inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010020">
        <property type="term" value="P:chloroplast fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034572">
        <property type="entry name" value="MCD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36317">
        <property type="entry name" value="PROTEIN MULTIPLE CHLOROPLAST DIVISION SITE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36317:SF1">
        <property type="entry name" value="PROTEIN MULTIPLE CHLOROPLAST DIVISION SITE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="105"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="297"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="33"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="298"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="316"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7736295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="342" mass="38836" checksum="47D9624163F648CE" modified="2024-05-29" version="1" fragment="single">HWVSTNALRPQLGFNKTWNSRNNKYHHHHHRGNSSSSSTSGGEDNNKSVVININKDEEEEVRLFVKPKSNSSTILLLQHQFSTLRQSLISSPPLLFLVKRSPSSIYMLGFCIATTVLIAAVRLYVERKSRYKRPGSVADLVRRGQLNSDRRGISKPVMYDDPFNNPQVKISKNNSTVEMCGKVYKLAPVTLTREEQGIHQKRRSRAYQWKRPTIFLKEGDPIPSDVDPDTIRWIPANHPFATTINDITEDLAQKNVYQKHGVPFRIQAEHEALQKKLEALQSDQKLNNLVIDPGAARNFERPFKSNSKLDDEQVEQKSTNGQTGSGQETQPSSPTSEEKQTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C7U7</accession>
    <name>A0AAD5C7U7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Miro domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">M8C21_015793</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">AA19_3_7</strain>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrial Rho GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009244">
        <property type="protein sequence ID" value="KAI7736560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C7U7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000553">
        <property type="entry name" value="Mitochondrial Rho GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052266">
        <property type="entry name" value="Miro-EF-hand_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020860">
        <property type="entry name" value="MIRO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46819">
        <property type="entry name" value="EF-HAND CALCIUM-BINDING DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46819:SF1">
        <property type="entry name" value="EF-HAND CALCIUM-BINDING DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51423">
        <property type="entry name" value="MIRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="12" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="471"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="domain" description="Miro" evidence="15">
        <location>
            <begin position="277"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="500"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAI7736560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="500" mass="55704" checksum="E710652AA9CAF1F1" modified="2024-05-29" version="1" fragment="single">GSLPVAPVAGNRTTVRVVVVGDRGTGKSSLIAAAASDLEGKAKLEEELKRADAVVLTYACDKPETLNRLQTFWIPEVRRLKVKAPVIVVGCKLDLRDEHFPVSLERVMAPIMQQFREIETCIECSAANLVQVPEVFYYAQKAVLHPTAPLFDQESQTLKPRCIRALKRIFILCDHDMDGALNDRELNEFQIKCFNAPLQPAEIVGVKRVVQERVPEGDGALQDSELDELFSTAPESPWDEAPYKNSVERTEFGDIRLSALRVTRKRTLDAKKQQSDRHVFQCFVFGPKNAGKTSLLSSFVGRPFQNTDGSTGNQSYTVNAVDQLRGTKKTLILHEIQEDDVKQFLSNKDSLAACDVAIFVYDSSDEYSLKRASALLMDVARRGEDTGYGVPCLFVALKDDLDSYPMAARDSDAICRELKIDAPIRISIKKREMNNIFWRIVNAAERCHLSVPETEYGRNMKQYRHLVNRSLVFASVGAAAAVIALAAYRAYASRKGHSAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C7W8</accession>
    <name>A0AAD5C7W8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Argonaute linker 1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_028069</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009236">
        <property type="protein sequence ID" value="KAI7736590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C7W8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014811">
        <property type="entry name" value="ArgoL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032474">
        <property type="entry name" value="Argonaute_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012442">
        <property type="entry name" value="DUF1645_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036085">
        <property type="entry name" value="PAZ_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33095:SF23">
        <property type="entry name" value="DUF1645 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33095">
        <property type="entry name" value="OS07G0619500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08699">
        <property type="entry name" value="ArgoL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16486">
        <property type="entry name" value="ArgoN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07816">
        <property type="entry name" value="DUF1645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01163">
        <property type="entry name" value="DUF1785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101690">
        <property type="entry name" value="PAZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Argonaute linker 1" evidence="2">
        <location>
            <begin position="94"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="364"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7736590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="438" mass="49201" checksum="114A94A83106294E" modified="2024-05-29" version="1">MKLLTSSFGAPHLGKLLLAYDGNKSAFVAGPLPFESKEFVVKITERNGRECEFKVNIKFAVKKDLHHLRQFLSGRQHYSPQETIQTLDVILRESASNGSLFIPQFGKGSLGDGIEYWKGFYQSLRPTQMGLSLNIVIEYYDLNIKGHWDGGESFDSLNSISPSFTGNSSNNNLADIAARVVEEFRNENGFEDDDDQDIYNFVTNDVNQNFEENDEEEDKEFEFAVVCRSNPNYSSHVSADEMFSNGEIVPKYPLFDRSLLLNNDDNDDVVSDKLKPDNNNNNNNNMISVSVRQPLGKLFREERETTPLSSEGGDDNLAGVIPGTYCIWKPKVESPEKCKKSNSTGNSSKRWRFRNLLQRSNSDGNMTASKHAPVNDFGHFPATKKKGDKTAKVAGADDDGEVRTVTAESNCEKKGGNRLRSYLRYKREQIGVSANVHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C839</accession>
    <name>A0AAD5C839_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Plasma membrane ATPase</fullName>
            <ecNumber evidence="18">7.1.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">M8C21_010875</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">AA19_3_7</strain>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The plasma membrane ATPase of plants and fungi is a hydrogen ion pump. The proton gradient it generates drives the active transport of nutrients by H(+)-symport. The resulting external acidification and/or internal alkinization may mediate growth responses.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17 18">
            <text>ATP + H2O + H(+)(in) = ADP + phosphate + 2 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:20852"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.1.2.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 18">Cell membrane</location>
            <topology evidence="2 18">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 18">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIIA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.1.2.1" evidence="18"/>
    <dbReference type="EMBL" id="JAMZMK010009446">
        <property type="protein sequence ID" value="KAI7735711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C839"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008553">
        <property type="term" value="F:P-type proton-exporting transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120029">
        <property type="term" value="P:proton export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02076">
        <property type="entry name" value="P-type_ATPase_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000045">
        <property type="entry name" value="ATPase 4 plasma membrane-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000004">
        <property type="entry name" value="Plasma membrane ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000004">
        <property type="entry name" value="Plasma membrane ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000211">
        <property type="entry name" value="Plasma membrane ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006534">
        <property type="entry name" value="P-type_ATPase_IIIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01647">
        <property type="entry name" value="ATPase-IIIA_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42861">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00120">
        <property type="entry name" value="HATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10 18" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="15 18" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 18" id="KW-0460">Magnesium</keyword>
    <keyword evidence="16 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13 18" id="KW-1278">Translocase</keyword>
    <keyword evidence="7 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 18" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="66"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="101"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="248"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="282"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="650"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="715"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="756"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="789"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="822"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="19">
        <location>
            <begin position="18"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU362083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAI7735711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="954" mass="105066" checksum="60B786155519064C" modified="2024-05-29" version="1">MAANSGISWDELTNENVDLETVPVNEVFETLKCTRDGLTTEEGNRRLSVFGPNKLEEKKESKLLKFLGFMWNPLSWVMEAAAIMAIVLANGGGKPPDWQDFVGITTLLIINSTISFIEENNAGNAAAALMAGLAPKTKVIRDGKWDEQDAAILVPGDVISVKLGDIIPADARLLEGDPLKIDQSALTGESLPVTKHPGDSVYSGSTCKQGEIEAVVIATGVHTFFGKAAHLVDNTNQVGHFQKVLTSIGNFCICSIAIGLIIEIVVMYPIQKRSYRNGIDNLLVLLIGGIPIAMPTVLSVTMAIGSHKLSEQGAITKRMTAIEEMAGMDVLCSDKTGTLTLNKLTVDKTLIEVFVKDIDKEQVILLGARASRVENQDAIDACIVGMLSDPKEARAGITEVHFLPFNPVDKRTAITYIDQSGNWHRVSKGAPEQIVELCNQKDDEKKKVFSIIDKFAERGLRSLAVCQQTVPEKTKESPGGPWVFVGLLPLFDPPRHDSAETIRRALHLGVNVKMITGDQLAIGKETGRRLGMGTNMYPSSSLLGQNKDASIADIPIEELIEKADGFAGVFPEHKYEIVKKLQERKHICGMTGDGVNDAPALKRADIGIAVADATDAARGASDIVLTEPGLSVIISAVLTSRAIFQRMKNYTIYAVSITIRIVLGFMLIALIWKFDFSPFMVLIIAILNDGTIMTISKDKVKPSPLPDSWKLKEIFATGVVLGTYLAVMTVVFFWLAKESDFFTEKFGVKPIRDNEYELMSALYLQVSIISQALIFVTRSRSWSFVERPGLLLLTAFFIAQLIATLIAVYANWNFARVHGIGWGWAGVIWLYSIVFYLPLDIFKFIIRFILSGKAWDNMLQNKTAFTSKKDYGRGEREAQWASAQRTLHGLQAPNASDILNDKSDYRELSELAEQAKRRAEVARLRELHTLKGHVESVVKLKGLDIETIQQHYTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C8W4</accession>
    <name>A0AAD5C8W4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Endoplasmin homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Glucose-regulated protein 94 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">M8C21_023964</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">AA19_3_7</strain>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the heat shock protein 90 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009201">
        <property type="protein sequence ID" value="KAI7736733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C8W4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16927">
        <property type="entry name" value="HATPase_Hsp90-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.80:FF:000006">
        <property type="entry name" value="endoplasmin homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11260:FF:000006">
        <property type="entry name" value="endoplasmin homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.790:FF:000005">
        <property type="entry name" value="Endoplasmin-like isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000005">
        <property type="entry name" value="Heat shock protein 90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.790">
        <property type="entry name" value="Heat shock protein 90, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00505">
        <property type="entry name" value="HSP90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019805">
        <property type="entry name" value="Heat_shock_protein_90_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037196">
        <property type="entry name" value="HSP90_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001404">
        <property type="entry name" value="Hsp90_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020575">
        <property type="entry name" value="Hsp90_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003555">
        <property type="entry name" value="PRK05218.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11528">
        <property type="entry name" value="HEAT SHOCK PROTEIN 90 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13589">
        <property type="entry name" value="HATPase_c_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00183">
        <property type="entry name" value="HSP90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002583">
        <property type="entry name" value="Hsp90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00775">
        <property type="entry name" value="HEATSHOCK90"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110942">
        <property type="entry name" value="HSP90 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00298">
        <property type="entry name" value="HSP90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042167289" description="Endoplasmin homolog" evidence="14">
        <location>
            <begin position="24"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase/HSP90-like ATPase" evidence="15">
        <location>
            <begin position="100"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="294"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="775"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="294"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="780"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="175"/>
            <end position="176"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="195"/>
            <end position="200"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="458"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00073396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00076587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR002583-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAI7736733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="819" mass="94068" checksum="801DF2283671354A" modified="2024-05-29" version="1">MRKWTLPSVLFLLCLLFLLPDHGRKLHANAESDADELVDPPKVEEKLGAVPHGLSTDSDVAKREAESMSRKNLRSNAEKFEFQAEVSRLMDILVNSLYSNKDIFLRELISNASDALDKIRFLSLTDKEVLGEGDDAKLEIQIKLDKENKILSIRDRGVGMTKEDLIKNLGTIAKSGTSAFVEKMQTGGDLNLIGQFGVGFYSVYLVADYVEVISKHNDDKQYVWESKADGAFAISEDTYNEPIGRGTEIRLHLREEAGEYLEESKLKDLVKKYSEFINFPIYLWATKEVDVEVPADEDDSSDDEEKPESTEGEEKEDEDTDKEEDEDKPKTKTVKETTYEWERLNDVKAIWLRSPKEVTEEEYTKFYHTLAKDFGDEKPMAWSHFNAEGDVEFKAVLFVPPKAPADLYESYYNSNKSNLKLYVRRVFISDEFDELLPKYLSFLLGLVDSDTLPLNVSREMLQQHSSLKTIKKKLIRKALDMIRKLAEEDPDEIHDKEKKEVEESKENDEKRGQYTKFWNEFGKSIKLGIIEDATNRNRLAKLLRFETTTSDGKLTSLDQYISRMKSGQKDIFYITGSSKEQLEKSPFLERLKKKNLEVIFFTDPVDEYLMQYLMDYEDKKFQNVSKEGLKLGKDSKDKEVKESFKELTKWWKEALATDNVDDVKISNRLADTPCVVVTSKYGWSANMERIMQSQTLSDASKQAYMRGKRVLEINARHPIIKELRERVVKDPEDASVKTTAQLMYQTALMESGFMLPDPKDFASRIYDSVKASLSISPDAAVEEEDEVEEAPEVENTSTEKGETDSTPTEEEHEDIKDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CDF8</accession>
    <name>A0AAD5CDF8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Alpha-soluble NSF attachment protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_031180</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Required for vesicular transport between the endoplasmic reticulum and the Golgi apparatus.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the SNAP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008550">
        <property type="protein sequence ID" value="KAI7739876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CDF8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005483">
        <property type="term" value="F:soluble NSF attachment protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035494">
        <property type="term" value="P:SNARE complex disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15832">
        <property type="entry name" value="SNAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000049">
        <property type="entry name" value="Alpha-soluble NSF attachment protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000744">
        <property type="entry name" value="NSF_attach"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13768:SF8">
        <property type="entry name" value="ALPHA-SOLUBLE NSF ATTACHMENT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13768">
        <property type="entry name" value="SOLUBLE NSF ATTACHMENT PROTEIN SNAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14938">
        <property type="entry name" value="SNAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00448">
        <property type="entry name" value="NSFATTACHMNT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 7" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="102"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7739876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="280" mass="31706" checksum="98DC967BF90616E1" modified="2024-05-29" version="1">MSDQIAKGLEFEKKAEKKLSGWGIFGSKQEDAADLYEKAANCYKLAKSWDQAGAVYMDSKHEAANAYANAGHSYKKTNTKECIACLEQALNVFMEIGRLSMSARYCKEIAELYEQEQNLEQAITYYDRAADLFQGEEVTTSANQCKQKIAQFSAQLEQYQKAINIYEEIARQSMNNNLLKYGVRGHLLNAGICQLCKGDVVAITNALERYQELDPTFSGTREYRLLADLAAALDEEDVVKFTDAVKEFDSMTKLDEWKTTLLLRVKLLLKAKEEEEDDLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CEM6</accession>
    <name>A0AAD5CEM6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_006230</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008530">
        <property type="protein sequence ID" value="KAI7740054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CEM6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053016">
        <property type="entry name" value="CTF18-RFC_complex"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46765">
        <property type="entry name" value="P-LOOP CONTAINING NUCLEOSIDE TRIPHOSPHATE HYDROLASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46765:SF1">
        <property type="entry name" value="P-LOOP CONTAINING NUCLEOSIDE TRIPHOSPHATE HYDROLASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7740054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="228" mass="26279" checksum="DCB3547B2D1DFCA8" modified="2024-05-29" version="1" fragment="single">MRTSSVALTALAEYTECDIRACLNTLQFLNKKKEMLNVLDISSQVIGRKDTSKSVFDIWKEIFQKRRLKGVRKSIDDCRSSFNEFTSLHSLVSNCGNYDLILDGIHENILQLNYHDPVMKKTVKCLHNLEISDILHQYVMRTQKMSLQVYQPAISIAIHGVVSQVEKPNIQWPKSFHRYRTSLIHKVEALHTWHNKITPCISRHISTKSFVADLVSPLLHILSPPTLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CEZ5</accession>
    <name>A0AAD5CEZ5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Exonuclease domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_017511</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">3'-5' exonuclease degrading single-stranded small RNAs.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the REXO1/REXO3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008641">
        <property type="protein sequence ID" value="KAI7739281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CEZ5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06145">
        <property type="entry name" value="REX1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000080">
        <property type="entry name" value="Small RNA degrading nuclease 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034922">
        <property type="entry name" value="REX1-like_exo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047021">
        <property type="entry name" value="REXO1/3/4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Ribonucl_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12801:SF115">
        <property type="entry name" value="FI18136P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12801">
        <property type="entry name" value="RNA EXONUCLEASE REXO1 / RECO3 FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00929">
        <property type="entry name" value="RNase_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00479">
        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exonuclease" evidence="8">
        <location>
            <begin position="157"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00053817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7739281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="509" mass="57082" checksum="3123AAA107EB726B" modified="2024-05-29" version="1">MKTQTKSLLDQKSQMKTYSMEKIEKAPKEVLVDIVKLAQKRGMEGSNGGWKEFLSVYDKKLGKSLSDPAKRAPDALLAFLNTFTNLDDLKFFDKVVEKHESTEHIEKKTDKLSPEQELVHKTVAHPHYALHYSFPSHEEGWVVTKNRKKLKETRCNTMVAVDCEMVLCEDGTDALVRVCVVNRDLKVKLDELVKPDKVVVDYRTSITGVSAKDLEQVTCSLQDIQESMKKLLSRGTILVGHSLNNDLQALKVDHTRVIDTSLVFKYWSGSNFRRPSILISVLGYELRKEGAPHNCLEDARAAMKLVLAKIDGGVDNNSIPTYQNDVHETEAMKLLCHRIPISIPGEKLLEIIPGEFTVELKANKKAGGDKYAAFAVFKNKQEADAAFDGLVGDQLKDSSGRPQKLVSFKLDSGVAGSLYVRKMVQDGDDNNNVQVASNKRPLEDGILTQPKKLKTDEETPEPKENVVCSNCDDYIKEIETLKKQLSDRDQEISMLHKIIVSQARKQSGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CFF5</accession>
    <name>A0AAD5CFF5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_033053</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008323">
        <property type="protein sequence ID" value="KAI7740926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CFF5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000468">
        <property type="entry name" value="PTI1-like tyrosine-protein kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056562">
        <property type="entry name" value="LysM2_CERK1_LYK3_4_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056563">
        <property type="entry name" value="LysM3_LYK4_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056561">
        <property type="entry name" value="NFP_LYK_LysM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052611">
        <property type="entry name" value="Plant_RLK_LysM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45927">
        <property type="entry name" value="LYSM-DOMAIN RECEPTOR-LIKE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45927:SF6">
        <property type="entry name" value="PROTEIN LYK5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23446">
        <property type="entry name" value="LysM1_NFP_LYK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23472">
        <property type="entry name" value="LysM2_CERK1_LYK3_4_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23473">
        <property type="entry name" value="LysM3_LYK4_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041973355" description="Protein kinase domain-containing protein" evidence="11">
        <location>
            <begin position="19"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="268"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="340"/>
            <end position="635"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7740926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="657" mass="72315" checksum="E434801B2998FC6F" modified="2024-05-29" version="1">MLRWFPAAFFFFLHGYNSQQTYVNNRQLDCNNNYTTASGYTCNGPTTCLSYITFRSQPPLYNTPTSIANLLNSNPTTITSLNNFSSISATIPADTVVVVPISNCSCSDGRLFYQHNASYVLRGTNETYFSVANNTYEGLSTCQAMIAQNPYNFRSLAVGNNITVPIRCACPTANQTAAGIRYLLTYLITWGDTYEYMSNLFEGVDVQGILDANELTASHIIFPFTPILIPLRSTPSRINITTNPAPPPAPPVIPVAPGTDDGGSSSKWVVVGVAIGVGLLLIVTLSGVLVWCFRRRKTQSLPLTKHSPTTALIPPESNTNSWSISTEGVRIAVGSLTVYKYEELQKATGDFSEENRVKGSVYRGVFNGDSAAVKIMKGDVSGEINILKQINHSNIIRLSGFCLHQGNTYLVYEFADNSSLSDWLHSTTKSTKYDSVLEWKQRVQIAHDIADALNYLHNFVTPPYIHKNLKSSNVLLDVHMRAKITNFGLARSVEETNDGELQLTRHVVGTYGYMPPEYIENGLITPKMDVFALGVLISELLSGKEAATRSLDNNGDEKDATELQERQLSEMVKDVVQGGDNVREKLAAFMDSRLSGEYPVELAYSMAEIASKCVAADLNERPSVAEVFATLSKILSSSLDWDPSDELEHSRSLSHGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CFI2</accession>
    <name>A0AAD5CFI2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 14">Peroxidase</fullName>
            <ecNumber evidence="2 14">1.11.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">M8C21_032075</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">AA19_3_7</strain>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 14">
            <text>2 a phenolic donor + H2O2 = 2 a phenolic radical donor + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:56136"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:139520"/>
            <dbReference type="ChEBI" id="CHEBI:139521"/>
            <dbReference type="EC" id="1.11.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11 14">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="11 14">Binds 2 calcium ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
        <text evidence="14">Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.7" evidence="2 14"/>
    <dbReference type="EMBL" id="JAMZMK010008452">
        <property type="protein sequence ID" value="KAI7740370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140825">
        <property type="term" value="F:lactoperoxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00693">
        <property type="entry name" value="secretory_peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.420.10:FF:000001">
        <property type="entry name" value="Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.420.10">
        <property type="entry name" value="Peroxidase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002016">
        <property type="entry name" value="Haem_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000823">
        <property type="entry name" value="Peroxidase_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033905">
        <property type="entry name" value="Secretory_peroxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31517">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31517:SF3">
        <property type="entry name" value="PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00141">
        <property type="entry name" value="peroxidase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00458">
        <property type="entry name" value="PEROXIDASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00461">
        <property type="entry name" value="PLPEROXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50873">
        <property type="entry name" value="PEROXIDASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11 14" id="KW-0106">Calcium</keyword>
    <keyword evidence="9 13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 14" id="KW-0349">Heme</keyword>
    <keyword evidence="14" id="KW-0376">Hydrogen peroxide</keyword>
    <keyword evidence="8 14" id="KW-0408">Iron</keyword>
    <keyword evidence="5 11 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3 14" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0964">Secreted</keyword>
    <keyword evidence="14" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041782796" description="Peroxidase" evidence="14">
        <location>
            <begin position="26"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="Plant heme peroxidase family profile" evidence="15">
        <location>
            <begin position="26"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="67"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="12">
        <location>
            <position position="63"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="36"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="69"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="125"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU362060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAI7740370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="283" mass="30988" checksum="FAA77AB65F58E8F9" modified="2024-05-29" version="1">MGWVTVIQVLALSSLIIMFPNLAFAQLKQNYYANVCPNVETIVRNAVTAKVRQTSVTIPGTLRLFFHDCFVQGCDASVMIASSGSNTAEKDHQENLSLAGDGFDTVIKAKAAVDAVPSCRNKVSCADILVMATRDVVVMVVTDFVVSCRRTHAGILPLRPIXXXXYNFSKQNQVDPTLSPSYAAQLQKQCPKNVDPRIAINMDPNTPNTFDNMYFKNLQQGKGLFTSDQVLFTDSRSKQTVNSWASNTQAFNNAFVAAMTKLGRVGVKNGKSGNIRRDCSAFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CHQ6</accession>
    <name>A0AAD5CHQ6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CTP synthase</fullName>
            <ecNumber evidence="9">6.3.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">UTP--ammonia ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_012352</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>UTP + L-glutamine + ATP + H2O = CTP + L-glutamate + ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:26426"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Pyrimidine metabolism; CTP biosynthesis via de novo pathway; CTP from UDP: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the CTP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.2" evidence="9"/>
    <dbReference type="EMBL" id="JAMZMK010008079">
        <property type="protein sequence ID" value="KAI7742096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CHQ6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003883">
        <property type="term" value="F:CTP synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044210">
        <property type="term" value="P:'de novo' CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019856">
        <property type="term" value="P:pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03113">
        <property type="entry name" value="CTPS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01746">
        <property type="entry name" value="GATase1_CTP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000305">
        <property type="entry name" value="CTP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004468">
        <property type="entry name" value="CTP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017456">
        <property type="entry name" value="CTP_synthase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033828">
        <property type="entry name" value="GATase1_CTP_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003792">
        <property type="entry name" value="PRK05380.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00337">
        <property type="entry name" value="PyrG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11550">
        <property type="entry name" value="CTP SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11550:SF42">
        <property type="entry name" value="CTP SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06418">
        <property type="entry name" value="CTP_synth_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 9" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="3 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 9" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CTP synthase N-terminal" evidence="11">
        <location>
            <begin position="10"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase" evidence="10">
        <location>
            <begin position="307"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU810713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF06418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7742096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="491" mass="54462" checksum="A1525FA563C369AE" modified="2024-05-29" version="1">MQKGNKKEMKYVLVTGGVVSGLGKGVTASSIGLLLQACNLRVTCIKIDPYLNTDAGTMSPFEHGEVYVLDDGGEVDLDLGNYERFLNIQLTRDNNITTGKIYQSVIDKERRGDYLGSTVQVVPHITDAIQDWIERVAAIPVDGKEGPPDVCVIELGGTIALGQFSYRVGAGNFCLIHVSLVPVLNVVGEPKTKPTQHSVRGLRSMGLIPNILACRSTTELDMNVKEKLSRFCHVPLENIVSLYDVPNIWHIPSLLRDQKAHEAILKALNLQSEVRQPNLKEWTAMAKRCDMLHEPVRVAMVGKYTDLSDSYLSVLKALLHASIVCRRKLVIIWVLATDLEDATAIENPDVHREAWNSLKAADAVVVPGGFGDRGVEGKINAAKYARENNIPYLGICLGMQIAVIEYARSVLGLKNANSTEFDPNTKNPCVIFMPELTNRVIVFVSDTSFIEERHRHRYEVNPDMVPQLEEAGLSFTGKDESGQRMQVRLFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CI61</accession>
    <name>A0AAD5CI61_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Zinc-ribbon domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_007634</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007977">
        <property type="protein sequence ID" value="KAI7742473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CI61"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900150">
        <property type="term" value="P:regulation of defense response to fungus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040244">
        <property type="entry name" value="EDR4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055126">
        <property type="entry name" value="EDR4-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021480">
        <property type="entry name" value="Zinc_ribbon_12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31105">
        <property type="entry name" value="EXTRA-LARGE G-PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31105:SF42">
        <property type="entry name" value="OS02G0258300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22910">
        <property type="entry name" value="EDR4-like_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11331">
        <property type="entry name" value="Zn_ribbon_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enhanced disease resistance 4-like N-terminal" evidence="3">
        <location>
            <begin position="23"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="domain" description="Probable zinc-ribbon" evidence="2">
        <location>
            <begin position="385"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="151"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="474"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="474"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="739"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7742473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="739" mass="82381" checksum="417BE63DCF39A4BD" modified="2024-05-29" version="1" fragment="single">SFVDYNGFSTTLPSGFLEEGSAVRLVRCPKCENLLPELPEYSVYQCGGCGAVLRGKQSTPASEKRPLERADNEIGTRVEPSTKVKVSLESSFQPERESDELEVGSKQGRIVHEKVPNLTPNSNSSARKEKRGIVTDSDQSRRRRLDYKYGMSSESQELRQPFGPIRSRPVGFHRNASTVEKGRFGNLPYSNEVTSSSHSSSYSSFGDHITNHGNGLHELPRVQDLENDRAELLKKLDDLRTQLSRSCNVAENPNGRLNGPYPWTRQLPPLTHQPPHHPYLPPTYHDPFLGYNQHDTLLHRPSCSCSQCYNMNWQPIPPKARPFSSFTSRDTSYQLPEPLSYGPCPLPRFHTGSLDLGNDGFGTRYPRRRIILSHGGERVSRPVSGGAPYISCCNCFELLKIPKKLTFEENNNQKTIKCAVCSALISLRLEDNKVVASAVSPIIQQKVNKRKSNLSSQNSLDYDKCDYNFQLTDTEPNVSTSGAEKSEEQSPDSVIDRPSSPVPILESRNDVLTKSQRTHHEKVIVDHNNKKSLKDALVATEIDVPLTEYLNCEVVSKEDDQLKNRKVGNESFFGGLIKKRLKDFSRSSSGVEKGRSDVSVNGQVLTDRAVKKAEKLAGPIHPGDYWYDSLAGFWGVMNQPCLGIIPPFIEEFDQPLPKTCSAGNTRVFVNGRELNQKDLDLLAGRGLPTTKDRSYIIDISGKVVDEDTGEELDALGKLAPTVERVKHGFGMKVPKVVTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CIG8</accession>
    <name>A0AAD5CIG8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box/LRR-repeat protein 15/At3g58940/PEG3-like LRR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_033985</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008038">
        <property type="protein sequence ID" value="KAI7742252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CIG8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050232">
        <property type="entry name" value="FBL13/AtMIF1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055411">
        <property type="entry name" value="LRR_FXL15/At3g58940/PEG3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31900">
        <property type="entry name" value="F-BOX/RNI SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31900:SF31">
        <property type="entry name" value="F-BOX_LRR-REPEAT PROTEIN 13-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24758">
        <property type="entry name" value="LRR_At5g56370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box/LRR-repeat protein 15/At3g58940/PEG3-like LRR" evidence="1">
        <location>
            <begin position="5"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF24758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7742252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="249" mass="28303" checksum="4F39359D04EC0930" modified="2024-05-29" version="1" fragment="single">VLIFELPLSLFTCKTLTKLRIAHGNPTSLVWECPCPVNLPCLKTLDIAVRDNPFVNAFELIRGCPALESLSLEVTIHKEEDYVFNIPTLKRLKLTLQRVWTSVTQKVVLNVPNLEYLFVGGMLGSLFVMEDLSSLVEASVSFHCIRSDHMCLEFLKGISGVKSLSIEKFEFTSAPPIFPNMNHLEFKSFWYSGLVLQYLESCPELKHLCIEKVLLQLVYNFGNFSVCEGFEPEIEFLKYMLGNAKILKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CIG9</accession>
    <name>A0AAD5CIG9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phospho-N-acetylmuramoyl-pentapeptide- transferase homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_007519</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008192">
        <property type="protein sequence ID" value="KAI7741470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CIG9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008963">
        <property type="term" value="F:phospho-N-acetylmuramoyl-pentapeptide-transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044038">
        <property type="term" value="P:cell wall macromolecule biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06852">
        <property type="entry name" value="GT_MraY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000715">
        <property type="entry name" value="Glycosyl_transferase_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003524">
        <property type="entry name" value="PNAcMuramoyl-5peptid_Trfase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00445">
        <property type="entry name" value="mraY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22926">
        <property type="entry name" value="PHOSPHO-N-ACETYLMURAMOYL-PENTAPEPTIDE-TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22926:SF5">
        <property type="entry name" value="PHOSPHO-N-ACETYLMURAMOYL-PENTAPEPTIDE-TRANSFERASE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00953">
        <property type="entry name" value="Glycos_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="145"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="184"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="226"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="255"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="287"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="324"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="355"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="381"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="405"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="485"/>
            <end position="506"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7741470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="509" mass="55421" checksum="8764036AE7A829A3" modified="2024-05-29" version="1">MRPSSLNLPRLGFLHPSIRSHQPVPNTFLTSNHHSRSLLCFKLLGSRVKRYGYSSTKGRFVVVCTMDWDSVSSFSLDDRVDSDGSVGYAFSSSEGEESDGDIILNPINDVDIPTSSERFRKPEDAITTTAYELRMMGKSHKRSRIKYGMIINIGLVTFLTVFLLLLDSHAWRIVRLPLPPFHLIFPYTVSAVLVSFAGYICVPLFRISRMQVIISKWPARHSFKKGTTTMGGLFLIPIGVVVAEVLVGFSSIEVAGASIATLAFATIGLIDDYLCLMKRRRIGLSPWIRILLEAAIGTWFYFWLCARNIASPYSIKMVVPLPLPLGLVCMGGSYVLLTSLCFVSMANGMELTDGLDGLAGGTAALAFIGMSIAVLPICSDLAVFGASMAGACVGFLFHNRYKASVFMGDTGSLALGGALAAMAASTGMFFPLLISSGVIVLEALSVLLQVSYFKTTKHVHGIGRRLFRMIPLHYHLESCGIKEPVIVAGAYMISCILMLYAGYVGLISV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CIQ2</accession>
    <name>A0AAD5CIQ2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Golgin candidate 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_008688</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008098">
        <property type="protein sequence ID" value="KAI7741975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CIQ2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012507">
        <property type="term" value="C:ER to Golgi transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005795">
        <property type="term" value="C:Golgi stack"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048211">
        <property type="term" value="P:Golgi vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048280">
        <property type="term" value="P:vesicle fusion with Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006955">
        <property type="entry name" value="Uso1_p115_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024095">
        <property type="entry name" value="Vesicle_P115"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006953">
        <property type="entry name" value="Vesicle_Uso1_P115_head"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10013">
        <property type="entry name" value="GENERAL VESICULAR TRANSPORT FACTOR P115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10013:SF0">
        <property type="entry name" value="GENERAL VESICULAR TRANSPORT FACTOR P115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04871">
        <property type="entry name" value="Uso1_p115_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04869">
        <property type="entry name" value="Uso1_p115_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Vesicle tethering protein Uso1/P115-like head" evidence="6">
        <location>
            <begin position="236"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="domain" description="Uso1/p115-like vesicle tethering protein C-terminal" evidence="7">
        <location>
            <begin position="659"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="489"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="613"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="636"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="489"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="510"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7741975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="784" mass="88128" checksum="5E58D8BDB823FDFB" modified="2024-05-29" version="1" fragment="single">EEDDFYIRYYTLQLLTALLTNSPIRLQEAILTIPRGITRLMDMLMDREVIRNEALLLLTYLTREAEEIQKILVFEGAFEKIFSIIKEEGGSEGGVVVQDCLELLNNLLRNNSSNQVLLRETIGFDSLISILKLRGTTYRFTQQKTINLLSVLETISLLLNGDRETNPASNTNVLTNKTILVQRKVLDHLLMLGVESQWAPVSVRCLVFQCIGDLISGHRQNLDALASKSLGDESEVEPALNSILRIILRTSSMQEFIAADYVFKRFCEQNTDGQKMLASTLIPHPMSFTNARFEEDVNMSFGSMLLHGLVMSEHDGDLEISSRAASVLSYVMKDNSQCKEKVLQIKLESLMPSLESPEPLLHRMVKYLALASSKKGKDGKSTTPRSSYFQPIILKLIVTWLYDCPNAVQSFLDSRPHLNYLIELVSDNESTVCARGLAAIVLGECVIYNKIIESGKDAFAIVDTISQKIGLTSYFLRLDEMQKSFIFSSAKSTQPQPRKPLTRSNANSMEEMENIDENESIDTRNDDHPMLASMFDSQFVNFIKSLEGGIRDSIVQIYSHPKSNVSVVPAELEQTKDENDSDYIKRLKLFVEKQCSEIQDLLNRNATLAEDLANTDGGGSVHTEPRPNNGSDRVQIETLRRDLQEASQRLEMLKTENMNIQSEALNYKKLAEKMETDLKSLSDAYDSLEQANYSLEMEVRTLKSGGATPSLDIEKIRAEAREEAQKESEGELGDLLVCLGQEQSKVEKLSARLMELGEDVDVLLEGIGEDDTGLLDDDDDEEEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CJ36</accession>
    <name>A0AAD5CJ36_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_015632</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008064">
        <property type="protein sequence ID" value="KAI7742170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CJ36"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16134:SF43">
        <property type="entry name" value="CORONATINE-INSENSITIVE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16134">
        <property type="entry name" value="F-BOX/TPR REPEAT PROTEIN POF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7742170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="369" mass="41785" checksum="71AF34A48C7FD574" modified="2024-05-29" version="1">MNHTQLEALNFYMTDLSQVSCNDLELIAKRCKSLVSVKIGDCEILDLVGFFRSAVSLEEFSGGCFNKQSEEYAALAYPPRLCRLGLNYMSTTEMPIVYPFASRLKKLDLLYALLDTEDHCVLLQRCPNLEVLETRNVIGDRGMEVLARSCKKMKRLRIERGADEQELEDEEGVVSQRGLTAIAQGCLELEYIAVYVSDITNAALESMGMNLKNLCDFRMVLLDKEEIITDLPLDSGIRSLLSGCNKLRRFALYLRPGGLTDVGLTYIGQNSQNIRWMLLGYLGESDSGLLGFSRGCPSLQKLEVRGCCFSENALAMAVLQLRSLRYLWVQGYRRSKTGFDLLAMARPYWNIEIIPPRKMEMYDLNIYSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CMG5</accession>
    <name>A0AAD5CMG5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CRC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_030713</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the lin-54 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007749">
        <property type="protein sequence ID" value="KAI7743299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CMG5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005172">
        <property type="entry name" value="CRC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033467">
        <property type="entry name" value="Tesmin/TSO1-like_CXC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044522">
        <property type="entry name" value="TSO1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46159:SF6">
        <property type="entry name" value="OS12G0605300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46159">
        <property type="entry name" value="PROTEIN TESMIN/TSO1-LIKE CXC 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03638">
        <property type="entry name" value="TCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01114">
        <property type="entry name" value="CXC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51634">
        <property type="entry name" value="CRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRC" evidence="5">
        <location>
            <begin position="254"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="115"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="127"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7743299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="329" mass="36779" checksum="B47925D1C275E024" modified="2024-05-29" version="1" fragment="single">MLQAMHVTWLSYLRYLRNLGTLTPPMPTLKREAAEAKERGKRRDGVYEHGQSIEWANVGCSSDCRCLGCENKFGKRTDFNVCNETIHETVENEMQSIGSGFPLHEFNNSHYSTPSTPVQQLGHENDPSTTGSYIPSPGSANSNTMLGTSRGDFEMVTSYQQAYLGAFMNQFTPPDTTHFEARHLNSVMNIPPTMDLPNSSTPSGSYFSAINRTGSLMNPVNEFGWPEVMNSNEKPNFGTRLDFTRMNSDSINGGKKPCRCKKSKCLKLYCECFAAGWYCDETCSCQECFNSPDFEDTVEETRKLIESKNPVAFSSKIRSSVESPTSQNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CNG9</accession>
    <name>A0AAD5CNG9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_017214</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protease inhibitor I3 (leguminous Kunitz-type inhibitor) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007300">
        <property type="protein sequence ID" value="KAI7745293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CNG9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004866">
        <property type="term" value="F:endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23375">
        <property type="entry name" value="beta-trefoil_STI_VvMLP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011065">
        <property type="entry name" value="Kunitz_inhibitor_STI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002160">
        <property type="entry name" value="Prot_inh_Kunz-lg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33107">
        <property type="entry name" value="KUNITZ TRYPSIN INHIBITOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33107:SF5">
        <property type="entry name" value="KUNITZ TRYPSIN INHIBITOR 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00197">
        <property type="entry name" value="Kunitz_legume"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00291">
        <property type="entry name" value="KUNITZINHBTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00452">
        <property type="entry name" value="STI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50386">
        <property type="entry name" value="STI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041937087" evidence="2">
        <location>
            <begin position="20"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7745293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="196" mass="21540" checksum="545578DE849236BE" modified="2024-05-29" version="1">MKISFFVFLVFSTLSLSSAQSTSAVLDINRNFIRYGARYYILPVISGRGGGIALTPTSINQKCPLNVVQENIDDRNGLPLSFIPANTKDAIIRESSDLNIMFPSVTICGKPPVWKLEAMNGQWFISSRGTLGSPGMNTLSNWFKIEKYGGNGYKLVYCPMVCNTCKPVCGDIGVAKNGRRSLILSKEPLIVMFKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CPM6</accession>
    <name>A0AAD5CPM6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Endoglucanase</fullName>
            <ecNumber evidence="10">3.2.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_000292</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>Endohydrolysis of (1-&gt;4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 8 10">Belongs to the glycosyl hydrolase 9 (cellulase E) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.4" evidence="10"/>
    <dbReference type="EMBL" id="JAMZMK010007242">
        <property type="protein sequence ID" value="KAI7745447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CPM6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008810">
        <property type="term" value="F:cellulase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000020">
        <property type="entry name" value="Endoglucanase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001701">
        <property type="entry name" value="Glyco_hydro_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033126">
        <property type="entry name" value="Glyco_hydro_9_Asp/Glu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018221">
        <property type="entry name" value="Glyco_hydro_9_His_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22298">
        <property type="entry name" value="ENDO-1,4-BETA-GLUCANASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00759">
        <property type="entry name" value="Glyco_hydro_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00592">
        <property type="entry name" value="GH9_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00698">
        <property type="entry name" value="GH9_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8 10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4 10" id="KW-0136">Cellulose degradation</keyword>
    <keyword evidence="6 8 10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3 8 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 8 10" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="73"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 9" evidence="13">
        <location>
            <begin position="111"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="515"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="563"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="572"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7745447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="621" mass="69122" checksum="76B065ABD2BB12AE" modified="2024-05-29" version="1">MSMYGRDPWGGPLEINNADSATDDDRSRNMNDFDRASLSRRLDETQQSWLLGPGEQKKKKYVDLGCVVVSRQIFVWTVGVLLAVGFVSGLVVLIVKTVPRHHKHPPPADNYTVALHKALMFFNAQKSGKLPRHNNVSWRGNSCMNDGKSDKSGAVLKDLVGGYYDAGDTIKFHFPKAFAMTMLSWSVIEYRAKYEAAGELNHVKEIIKWGTDYFLKTFNSTADSISQLVAQVGKGDTSGGTSDPNDHTCWMRPEDIDYPRPVTECSSCSDLAAEMAAALASASIVFKDNKVYSKKLVHGAATLWKFARDQRGSYSAGGSDAATFYNSSMYWDEFVWGGAWMYYATGNQSYLYLASHPKLAKHAGAFWGGPDYGVLSWDNKLTGAQVLLTRLRLFLSPGYPYEETLKTFHNQTSIIMCSYLPYFNSFNRTRGGLIQLNHGRPQPLQYVVNAAFLATLYSDYLDAADTPGWYCGPNFYSTNELRKFAESQIDYILGKNPRKMSYVVGYGNHYPKHVHHRGASIPKNKVKYNCKGGYKWRDSKKPNPHTIIGAMVAGPDKHDGFHDVRTNYNYTEPTLAGNAGLVAALVALSGDKTTKIDKNTIFSAVPPMFPTPPPPPAPWKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CR00</accession>
    <name>A0AAD5CR00_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ribosomal protein S35, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_030238</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006926">
        <property type="protein sequence ID" value="KAI7746658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CR00"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052851">
        <property type="entry name" value="GCD1_mitochondrial"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35476">
        <property type="entry name" value="MUCIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35476:SF3">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS75"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12298">
        <property type="entry name" value="Bot1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7746658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="359" mass="41010" checksum="FCEEEAE350519DCD" modified="2024-05-29" version="1">MRLLTHLISQLSTNSRATQLKTVTNTVTYNFLSSSRFLSTKGGNEGEVDWGSESTWSSGLTKDHFDGEVVGEKVSDTAAGATGGAAGGAAGNVGGGGPTFNSSHEDDLANIRKLAAAASRKDAEFASKWKDRMHETNMLMKQVLEPGARGAYLKDSEKAEMYRLHKENPEVYTVEKLAKDYRIMRQRVHAILWLKEDEEKMEKKLGHPLDDSVEQLLDNFPEFFEWHDREFHVATLPYKPDFKVMPEGWDGTIKDPDEVLYEISMKEDEILYQEFLERFNFNKMKIEGKVKVHKYSRRRPSEGWEITVEKMGPRGKRGDGGGWKFKSLADGSTRPLNDYEKMFVKREKPRRRRKILHPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CRM2</accession>
    <name>A0AAD5CRM2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_033953</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DExH box helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="JAMZMK010007078">
        <property type="protein sequence ID" value="KAI7745965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CRM2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000819">
        <property type="entry name" value="ATP dependent RNA helicase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059023">
        <property type="entry name" value="RNA_hel_CTD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF246">
        <property type="entry name" value="DEXH-BOX ATP-DEPENDENT RNA HELICASE DEXH4, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26026">
        <property type="entry name" value="RNA_hel_CTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase C-terminal" evidence="9">
        <location>
            <begin position="172"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="95"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00060772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7745965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="680" mass="76669" checksum="BD873193DC230E12" modified="2024-05-29" version="1" fragment="single">GNKSFADITHVIVDEVHERSLLGDFLLIILKNFIEKQSAQRTQQKLKVILMSATVDSQLFSHYFGDCPVIHAQGRTHPVTTHFLEDIHETIDYRPASDSPASLRSDTPKQKGAAVDNHRGKKNLVLSAWGDESVLSKGYSNPYYFAENYQTYSEQTQQNLKKLNEDVIDYDLLEDLICHVDETYPEGAILVFLPGVSEIHTLLDKLAASYQFRGAASEWLLPLHSSIASSEQKKVFLRPPDDIRKIIIATNIAETSITIDDVVYVIDCGKHKENRYNPRKKLSSMVEDWISRANARQRRGRAGRVKPGICFCLYTRHRFEKLMRPFQLPEMLRMPLVELCLQIKLLSLGRIMPFLQKALEPPTEEAITSAISLLYEVGAVEGDEELTPLGHHLAKLPVDVLIGKRNAVERAKLALLTDKSGGANDVDNGDRQSDHLIMMIAYKKWEKILRENGSNAAERDMRVQLGTLLADIGYIELPKNYQIGGKWKEKLDSWFSDPSQTFNVYSNHSSVVKALLCAGLYPNVAATEQGISEKALNSLRQSLGPATDDLNVWFDGRRVVHIHPSSVNSNLKTFQHPFLVFLEKVETTKVFLRDTTITSPYSILLFGGSMNIQHQTGLVIIDGWLKMAAPAQTAVIFKELRLTLHSILKELIRKPQVTTDPELQISRLSFFDPVFKPVSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CSJ0</accession>
    <name>A0AAD5CSJ0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">M8C21_022208</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="23">AA19_3_7</strain>
            <tissue evidence="23">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010007154">
        <property type="protein sequence ID" value="KAI7745731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CSJ0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.430:FF:000002">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000140">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001023">
        <property type="entry name" value="Os07g0541700 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000298">
        <property type="entry name" value="Putative LRR receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.430">
        <property type="entry name" value="Galactose-binding lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051824">
        <property type="entry name" value="LRR_Rcpt-Like_S/T_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021720">
        <property type="entry name" value="Malectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48006:SF62">
        <property type="entry name" value="LEUCINE-RICH REPEAT TRANSMEMBRANE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48006">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN DDB_G0281931-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11721">
        <property type="entry name" value="Malectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="14 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-0675">Receptor</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8 21" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042142956" description="non-specific serine/threonine protein kinase" evidence="21">
        <location>
            <begin position="36"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="545"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="22">
        <location>
            <begin position="605"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="888"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="892"/>
            <end position="907"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="KAI7745731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="921" mass="101258" checksum="BA6C9A067DFB3D7A" modified="2024-05-29" version="1">MEATAAGGDGNRIKLRNFVVASWLWLIIMATIAQAQNTTLTDPAEARVIHEMFRQWGIKESEVANMRWNLSGEPCSGAAVDSTTFDNNTRVYSMDAVGLIPEGLWTLVYLTNLTMGINALSGELPPELGQLSDLRLLGIGTNNFNGSVPSELGNLRRLQQLAYLYTMSLNKSFSYIDSSGVGGGLRATLSKLLGCYECCRRISGLSNGTLDFIKDLKSLGVLVLRNNKISGSIPTDIGEHHNLTRLDLSFNDLNGEIPRGLFNLSQLSFLFLGNNSLNGILPDVKSASLRNIDVSYNELSGTLPSWVNEANLQLNLVVNNFTLGDSGNSGLPSDSDFGINCGGPPITSSNQLLHEEDREALGATTYYVTSERRWGVSNVGQSDRPRFTGSSSRQFTGTLDSELFQTARLSAGSLRYYGLGLENGNYTVTLQFAELEIEDGRTWKSTGRRVFDIYLQGNLAFQDFDIRRAAGGAAFRPVSRQATVHVTDNYLEIHIFWSGKGTCCVPKQGTFGPLISAISATPNFRPSVSNTPPSTKKNGKNRTGLIVGIVVPIAFVSFLSLLVLYIFRQRRKKQDAYYKYEEFFGIDTRPDTFSYGDLRDATDGFSPANKLGEGGFGPVYKGTLNDGRVIAVKQLSVASHQGKGQFIAEISTISAVQHRNLVKLYGCCIEGDKRLLVYEYLENNSLDHALFGDNRLFLSWATRFEICLGIARGLAYLHEESRMRVVHRDVKASNILLDSELNPKISDFGLAKLYDDKKTHMSTQKADVFGFGVVALEIISGRPNSDSSLEDEKVYLLEWAWNLHETNSEVELVDEELSEYDENEVKRVTRIALLCTQTSPTQRPSMSQVVVMLSGDIEVVSVITRPGYLSGFNFRDTTTFKSAASTLGAGSMPSTGQSTVLPSSPDNPSRLMLHNVIGEGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CSU4</accession>
    <name>A0AAD5CSU4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_020570</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007076">
        <property type="protein sequence ID" value="KAI7745970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CSU4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7745970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="93" mass="10688" checksum="FBFAC0C9A3AE3DF1" modified="2024-05-29" version="1">MRIAMKQYNRPPSARLNVKVALKGVPEVKELRTRGTKDPLERMLKLCLLELKISVFAAENWRFAAEKLPQILPYTTALNRNLRLPPVGYGWVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CSX4</accession>
    <name>A0AAD5CSX4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glycosyltransferase</fullName>
            <ecNumber evidence="5">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_009263</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="5"/>
    <dbReference type="EMBL" id="JAMZMK010006916">
        <property type="protein sequence ID" value="KAI7746700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CSX4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035251">
        <property type="term" value="F:UDP-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000047">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000071">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058980">
        <property type="entry name" value="Glyco_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48047">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48047:SF229">
        <property type="entry name" value="UDP-GLYCOSYLTRANSFERASE 73C3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26168">
        <property type="entry name" value="Glyco_transf_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyltransferase N-terminal" evidence="6">
        <location>
            <begin position="10"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF26168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7746700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="455" mass="50907" checksum="D9F5D83AE9ACC860" modified="2024-05-29" version="1" fragment="single">MASHHTHLHFVLFPLMTQGHMIPMFDIARLLAERGSMVTIITTPVNASRFKPVIDRAINDQLKIQILELQLPLTQVGLPEGCENFDLIQSAQLFPKLFEAASMLEEPAKNMLQGLTPPPSCIISDNLFSWTNSIARNLSIPRLAFHGPGCFTCLCIHISIYTNVLDDIASDSEYFVLPGIPDRIELTQLQASSWGGKGLEEAKAFVEVIKKAEQDASGIVVNSFLELEPYYVEELAKVKDKKVWCIGPVSLCNKNLLDIAERGSKGPINKYDCLKWLDSMETESVVFACLGSLARVCTEQNIDLGLGLESSNKPFLWCVRHATEEFERWLLNEGYEERVKDRGLIIRGWAPQVLILSHRAVGGFITHCGWNSILEGVSAGVPMVTWPHFADQFINERFIVDVLKIGVKIGMEIPVVVGEQDKFGVMVNKEDIKRSVEDLMDKGKEVVRMPFSSLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CTB2</accession>
    <name>A0AAD5CTB2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GRF-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_018572</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006876">
        <property type="protein sequence ID" value="KAI7746910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CTB2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010666">
        <property type="entry name" value="Znf_GRF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33248">
        <property type="entry name" value="ZINC ION-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06839">
        <property type="entry name" value="Zn_ribbon_GRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51999">
        <property type="entry name" value="ZF_GRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GRF-type" evidence="6">
        <location>
            <begin position="6"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="57"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7746910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="112" mass="12615" checksum="02DA97BD658C0E34" modified="2024-05-29" version="1">MAPIYCRCGREAIVRTSWTSKNPGRRFYSCPIKASQCGGFLCWVEPDNVIAGLLRSKNELEGSITELQGSISELEAVNEVLVDKLKAKEDESRKRKKYLVLSWVFFCGCVCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CTI6</accession>
    <name>A0AAD5CTI6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_027915</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006619">
        <property type="protein sequence ID" value="KAI7747991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CTI6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7747991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="124" mass="14137" checksum="0F558CB6BEADE4F1" modified="2024-05-29" version="1" fragment="single">FVSVRCHCSFAAGCRSRFWTFGWCIEHETWKSALHYLWMIVHDHRLLRADIPVLEVFRVQSALRSEDEAYRRLHVGLIIVAAQNDACVLRIGAIDGEVSITFLMTVIIVILLGRAVDHPSAEPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CTS1</accession>
    <name>A0AAD5CTS1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Flavin mononucleotide hydrolase 1, chloroplatic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_008312</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006695">
        <property type="protein sequence ID" value="KAI7747749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CTS1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01549">
        <property type="entry name" value="HAD-SF-IA-v1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01509">
        <property type="entry name" value="HAD-SF-IA-v3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43611">
        <property type="entry name" value="ALPHA-D-GLUCOSE 1-PHOSPHATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43611:SF3">
        <property type="entry name" value="FLAVIN MONONUCLEOTIDE HYDROLASE 1, CHLOROPLATIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7747749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="255" mass="29276" checksum="92F6253D2CC55164" modified="2024-05-29" version="1">MAALIPPSKSYQLITTTSSNILPFICSRSPSSPKLPATKMSLNTSITPRKLPVLLFDIMDTIVRDPFYHDIPAFFGMSMKELLDCKHPTAWIEFEKGLIDEMELARIFFKDGRHFDLEGLKSCIYQGYSYLEGVEELLSTLKDNGYEMHAFTNYPIWYRMIEDKLKLSSYLSWTFCSCTIGKRKPDPHFYSEVLQRLDTEPQNCVFIDDRLKNIEAAKEAGINGIQFKNADSLQQDLSLLGITFQNKTKTQEHEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CW70</accession>
    <name>A0AAD5CW70_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Eukaryotic translation initiation factor 2A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_016126</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat EIF2A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006714">
        <property type="protein sequence ID" value="KAI7747636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CW70"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011387">
        <property type="entry name" value="TIF2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013979">
        <property type="entry name" value="TIF_beta_prop-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13227">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13227:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08662">
        <property type="entry name" value="eIF2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="4" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Translation initiation factor beta propellor-like" evidence="9">
        <location>
            <begin position="296"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="403"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="404"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="424"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7747636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="483" mass="52914" checksum="FF375830CF35D465" modified="2024-05-29" version="1">MSVADSSPPLEILVREPGGFAIWNGPPFSNNQPSIKLDKVPCTSAKFSEDGSKLMVIKSESAISIYDCKTLNEIRCFESPNVLAAALSPCGTYLQTFQKASSPQEKNVTLWKTETGESVYALSQKNMTKATWPSIRFSSDEAVASRMATNELQFFDSGDFSKGVVHKIRVPGVASAELSNNPGSHVAAFVPESKGMPGSVQIYACAPDSQSEPIARRSFFRCSSVQQHWNNGSTGLLVVAQADVDKTNQSYYGETKLYYLTLDGSHDGLVSLRKEGPVHDVQWSYSGKEFAVVYGFILVAGYGNLPGEMAFWDYVEKKQLGTTKAEWSVTSEWSPDGRYFMTATTAPRLQVDNGIKIFHYNGKSLFKKKFDKLYQVDWKAESPDKFGDIEELIKSVDSLKVESKSQGQGSKSSQAKPINPPVQKKPAAYQPPHAKAKAAKEAELFGGSPSVEMSKNALKNKKKREKQREKKAAEAGSAVNGAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CWN5</accession>
    <name>A0AAD5CWN5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_010891</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006291">
        <property type="protein sequence ID" value="KAI7749723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CWN5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34567:SF11">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34567">
        <property type="entry name" value="FK506-BINDING-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="236"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="277"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7749723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="288" mass="33429" checksum="CD1821B5A6CE270C" modified="2024-05-29" version="1">MDSWKDQGYHFTPEVTRTRSSRYRKPPVGSSSNWQQTVPSWEKQFCSSVGSIPWNKILETKKTIHLYDNIIKWNDSAGKEAFEKAKNNFYATMHNLPSNSGSLDADIYIDNINWDSEVDENLMHDLDSDCVVPDSNSKSKDEHVVIFGSSFPPSYQSFSPYGWGDSDDDKKKDIISPVYNLHQWKIEGGSFDKNDNWRVSNENDGRGDSRNDYWGWNMYDDNNCFYDDVKNEPKEGSGRYMSRYKTSRFRKDNDRARNNGNQRKSGYSCEPVNPSSHAHRWSVKKPFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CZL3</accession>
    <name>A0AAD5CZL3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Solute carrier family 40 member</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_013009</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">May be involved in iron transport and iron homeostasis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ferroportin (FP) (TC 2.A.100) family. SLC40A subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the major facilitator superfamily. Phosphate:H(+) symporter (TC 2.A.1.9) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006093">
        <property type="protein sequence ID" value="KAI7750707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CZL3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005381">
        <property type="term" value="F:iron ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009716">
        <property type="entry name" value="Ferroportin-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11660">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 40 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11660:SF57">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 40 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06963">
        <property type="entry name" value="FPN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="60"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="93"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="128"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="182"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="298"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="237"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="247"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="259"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU365065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7750707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="343" mass="38161" checksum="BC385E8DBBC9ECC3" modified="2024-05-29" version="1" fragment="single">MEDRYYVRLEDEHHHQLQDPVPTSLLVYLYIGHFLARWGARTWEFSVGLYMINVWPDSLLMAAAYGVVESATTAVFGPLVGQWIDNSTYPKVLRVWLLTQNLSFIVAGVTVVGLLISPDMRVTNATTFSMLVVLTNLSGAVAVLSTLAGTILIEREWVVVISEGHSSNLLTKLNSMIRRIDLISTLFAPVVTGFIISFVSMIASAVGLAIWNILSYWFLNSVYKGIPALQERSRKRGLKLKTKPNDQEQAPSTSQEETNQNHDSSDDVASRSLVRKFVERVSESPFIHAWQVYLQQDIVLAGLSLALLYFTVLSFGTLMTATLEWDGIPAYIIGTGRGISAIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D041</accession>
    <name>A0AAD5D041_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Adenylate kinase isoenzyme 6 homolog</fullName>
            <shortName evidence="10">AK6</shortName>
            <ecNumber evidence="10">2.7.4.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Dual activity adenylate kinase/ATPase</fullName>
            <shortName evidence="10">AK/ATPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_021922</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Broad-specificity nucleoside monophosphate (NMP) kinase that catalyzes the reversible transfer of the terminal phosphate group between nucleoside triphosphates and monophosphates. Has also ATPase activity. Involved in the late cytoplasmic maturation steps of the 40S ribosomal particles, specifically 18S rRNA maturation. While NMP activity is not required for ribosome maturation, ATPase activity is. Associates transiently with small ribosomal subunit protein uS11. ATP hydrolysis breaks the interaction with uS11. May temporarily remove uS11 from the ribosome to enable a conformational change of the ribosomal RNA that is needed for the final maturation step of the small ribosomal subunit. Its NMP activity may have a role in nuclear energy homeostasis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>AMP + ATP = 2 ADP</text>
            <dbReference type="Rhea" id="RHEA:12973"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="10">Interacts with small ribosomal subunit protein uS11. Not a structural component of 43S pre-ribosomes, but transiently interacts with them by binding to uS11.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the adenylate kinase family. AK6 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.3" evidence="10"/>
    <dbReference type="EMBL" id="JAMZMK010006341">
        <property type="protein sequence ID" value="KAI7749555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D041"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004017">
        <property type="term" value="F:AMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000372">
        <property type="entry name" value="Adenylate kinase isoenzyme 6 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00039">
        <property type="entry name" value="Adenylate_kinase_AK6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020618">
        <property type="entry name" value="Adenyl_kinase_AK6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12595:SF0">
        <property type="entry name" value="ADENYLATE KINASE ISOENZYME 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12595">
        <property type="entry name" value="POS9-ACTIVATING FACTOR FAP7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13238">
        <property type="entry name" value="AAA_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="4 10" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="5 10" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="NMPbind" evidence="10">
        <location>
            <begin position="34"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="LID" evidence="10">
        <location>
            <begin position="109"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="10"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7749555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="171" mass="19348" checksum="243ED498BEB2A165" modified="2024-05-29" version="1">MRSKPNILVTGTPGTGKTTTSSALAEATHLRHINIGDLVKDKKLHDGWDDDFQCHVINEDLVCDELEDIMEEGGVIVDYHGCDFFPERWFDRVVVLQTETSVLYDRLSKRGYAGAKLTNNIECEIFQELLEEAKESYKEEIVEAMKSDTVEDMNRNVAAITEWVSSWSSTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D0J6</accession>
    <name>A0AAD5D0J6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Sorting nexin C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_008702</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006134">
        <property type="protein sequence ID" value="KAI7750529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D0J6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013937">
        <property type="entry name" value="Sorting_nexin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051837">
        <property type="entry name" value="SortingNexin/PXDomain-PKLike"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22999:SF35">
        <property type="entry name" value="PHOX DOMAIN, SORTING NEXIN, PX DOMAIN SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22999">
        <property type="entry name" value="PX SERINE/THREONINE KINASE PXK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08628">
        <property type="entry name" value="Nexin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sorting nexin C-terminal" evidence="4">
        <location>
            <begin position="123"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="184"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="189"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7750529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="293" mass="33024" checksum="6A902CD1C9CA31A2" modified="2024-05-29" version="1" fragment="single">NVDEGIMRNVMGPCLPSKSSSSVVNKNVSLKTDSVTKITTSDLAKSFSENEEGEKDRLLSDEEKVGPAAQGNVQILEKSENLPSASGLKEDPLGVPAEWSPSNVSVPLLNLVDKIFQLKRRGWLRRQVFWISKQILQLMMEDAIDDWLLRQIHFLRRDNIVAQGIHMIQDILWPEGTFFLKSRTSQTDSSQSNDRSANNNNNKQGSFEEQLEAARRASDIKKMIFKGAPTTLVSLIGRKQYKRSAKDVYYFLQSAVCLKQLGYGILELALMAIFPELQGIILNVHEKKNEQVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D0M6</accession>
    <name>A0AAD5D0M6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">H15 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_012810</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the histone H1/H5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005829">
        <property type="protein sequence ID" value="KAI7751728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D0M6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031492">
        <property type="term" value="F:nucleosomal DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030261">
        <property type="term" value="P:chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045910">
        <property type="term" value="P:negative regulation of DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00073">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005819">
        <property type="entry name" value="H1/H5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005818">
        <property type="entry name" value="Histone_H1/H5_H15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11467">
        <property type="entry name" value="HISTONE H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11467:SF131">
        <property type="entry name" value="HISTONE H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00538">
        <property type="entry name" value="Linker_histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00624">
        <property type="entry name" value="HISTONEH5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00526">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51504">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="4 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="H15" evidence="8">
        <location>
            <begin position="26"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="94"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="104"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="129"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="144"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="164"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="197"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7751728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="219" mass="22686" checksum="894C9182AFA5E3C3" modified="2024-05-29" version="1">MATAEPVVDTKVKKTKKPSAPKNPPQHPTYFEMIKEAIVTLKERTGSSSQAIGKFIEGKYKSLPANYKKMLSTQLKKNVADGKLVKVKASFKLPPKVPAGSAKPAKKPAAKPKKAPVKKKAVATPTKAKAVKAKPAAKAKPAAKPKAVAATKAKPAAGKRKAAAKPAAKPAAKVAKTSTRATPKKAAAVKVKKTPVKKAAAAKKTAAPKKAAAAKKGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D0P3</accession>
    <name>A0AAD5D0P3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">phosphatidylglycerophosphatase</fullName>
            <ecNumber evidence="10">3.1.3.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">M8C21_002618</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">AA19_3_7</strain>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Exhibits phosphatidylglycerophosphate phosphatase activity. Involved in root growth and columella cells organization. May possess protein phosphatase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycero-3'-phosphate) + H2O = a 1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycerol) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:33751"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:60110"/>
            <dbReference type="ChEBI" id="CHEBI:64716"/>
            <dbReference type="EC" id="3.1.3.27"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:33752"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="9">Phospholipid metabolism; phosphatidylglycerol biosynthesis; phosphatidylglycerol from CDP-diacylglycerol: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.27" evidence="10"/>
    <dbReference type="EMBL" id="JAMZMK010005794">
        <property type="protein sequence ID" value="KAI7751891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D0P3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008962">
        <property type="term" value="F:phosphatidylglycerophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004721">
        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048364">
        <property type="term" value="P:root development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14524">
        <property type="entry name" value="PTPMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000051">
        <property type="entry name" value="Dual specificity phosphatase domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044596">
        <property type="entry name" value="PTPMT1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46274:SF9">
        <property type="entry name" value="PHOSPHATIDYLGLYCEROPHOSPHATE PHOSPHATASE PTPMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46274">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="5" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="13">
        <location>
            <begin position="58"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="14">
        <location>
            <begin position="119"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00050944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00053902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAI7751891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="325" mass="36224" checksum="011AFD690EABAB2F" modified="2024-05-29" version="1">MHIEDLKGVDESSGGEGNGAIGLSDSKRVLVGAGARALFYPTLLYNVVRNRFEPEFRWWDQIEEFLLLGAVPFPSDVKRLKDLGVRGVVTLNEPYETLVPTSLYRAHGIDHLVLPTRDYLFAPSLDDISEAVNFIHGKTLLKQATYVHCKAGRGRSTTIVICYLIQHRHMHPDAAYAYVRSIRPRVLLASAQRQAVEEYYFLKVKKMQSPGNIWSSCSSRSCNTICKPLSFYPAAADVFTFDDGSVVVIAKADLDGYEDEDEKRVGGRQIWSGVNIVCRVGVATLARFSSLLHSSSKISSVSFVTRKDGYSSKTTHDVSINIHLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D0T3</accession>
    <name>A0AAD5D0T3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Aminotransferase class I/classII large domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_007313</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006237">
        <property type="protein sequence ID" value="KAI7749975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D0T3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008793">
        <property type="term" value="F:aromatic-amino-acid transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004069">
        <property type="term" value="F:L-aspartate:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050478">
        <property type="entry name" value="Ethylene_sulfur-biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43795:SF85">
        <property type="entry name" value="AMINOTRANSFERASE ACS10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43795">
        <property type="entry name" value="BIFUNCTIONAL ASPARTATE AMINOTRANSFERASE AND GLUTAMATE/ASPARTATE-PREPHENATE AMINOTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00753">
        <property type="entry name" value="ACCSYNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="2">
        <location>
            <begin position="3"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7749975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="319" mass="35753" checksum="3AD92BEFCDA95DBC" modified="2024-05-29" version="1">MNGITAYHPFDGLSELKMAVAGFMSQVMEGRVLFDPSHMVLTSGVTPAIEMLVFCLADLGNAFLVPTPYSPDLDRDIKWRTGVEIIPVPCRSSDNFSLSTASLNRAYSQAKKRGLKVQGVIVSNPSNPVGTLLTRETLYNLLDFATEKNIHVIANETLVGPIDDKTQEFVSMAEIISSEEYDRKRVHIIYGLSKDISLSGFELGVVYSFNQTVLQASKKLMRFSSVSSLSQRLLISMFNDSRFVRDIININKERVVRMSDLFVGGLKSLGIKCMKSSRGSYCWADMSGFIRPYSEKGELELWEKGQNCKMIRKKKERTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D1C5</accession>
    <name>A0AAD5D1C5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 8">Pectinesterase</fullName>
            <ecNumber evidence="2 8">3.1.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_026915</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6 8">
            <text>[(1-&gt;4)-alpha-D-galacturonosyl methyl ester](n) + n H2O = [(1-&gt;4)-alpha-D-galacturonosyl](n) + n methanol + n H(+)</text>
            <dbReference type="Rhea" id="RHEA:22380"/>
            <dbReference type="Rhea" id="RHEA-COMP:14570"/>
            <dbReference type="Rhea" id="RHEA-COMP:14573"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17790"/>
            <dbReference type="ChEBI" id="CHEBI:140522"/>
            <dbReference type="ChEBI" id="CHEBI:140523"/>
            <dbReference type="EC" id="3.1.1.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.11" evidence="2 8"/>
    <dbReference type="EMBL" id="JAMZMK010006056">
        <property type="protein sequence ID" value="KAI7750934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D1C5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030599">
        <property type="term" value="F:pectinesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042545">
        <property type="term" value="P:cell wall modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045490">
        <property type="term" value="P:pectin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033131">
        <property type="entry name" value="Pectinesterase_Asp_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000070">
        <property type="entry name" value="Pectinesterase_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31707">
        <property type="entry name" value="PECTINESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01095">
        <property type="entry name" value="Pectinesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00503">
        <property type="entry name" value="PECTINESTERASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 8" id="KW-0063">Aspartyl esterase</keyword>
    <keyword evidence="5" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pectinesterase catalytic" evidence="9">
        <location>
            <begin position="2"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7750934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="142" mass="16148" checksum="4BBAE543231619FE" modified="2024-05-29" version="1">MVIVYTKQGLYEENVEIPSYKTNIVIFGEGSDMTMITGNRSVMDGWTTFRFATVVVSGEGFLAHDIGFHNLAGPEKHQAIVLRIKADFAAVYRCSISSYQDTFYAHSSRQFYRECDIYGAIDYIFGNATMVFRRQLKRTGAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D202</accession>
    <name>A0AAD5D202_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_001163</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006114">
        <property type="protein sequence ID" value="KAI7750621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D202"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7750621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="32" mass="3958" checksum="EEEC287BF7AFDA00" modified="2024-05-29" version="1">MLHSEGQTCQQVQRRRVRSTRHKYFLELLWLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D243</accession>
    <name>A0AAD5D243_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Polycomb protein VEFS-Box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_033826</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the VEFS (VRN2-EMF2-FIS2-SU(Z)12) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005629">
        <property type="protein sequence ID" value="KAI7752731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D243"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd21553">
        <property type="entry name" value="VEFS-box_EMF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21749">
        <property type="entry name" value="ZnB-Zn_EMF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056068">
        <property type="entry name" value="EMF2-like_DUF7651"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019135">
        <property type="entry name" value="Polycomb_protein_VEFS-Box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057540">
        <property type="entry name" value="Znf_SUZ12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22597">
        <property type="entry name" value="POLYCOMB GROUP PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22597:SF22">
        <property type="entry name" value="POLYCOMB GROUP PROTEIN EMBRYONIC FLOWER 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24663">
        <property type="entry name" value="DUF7651"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09733">
        <property type="entry name" value="VEFS-Box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23320">
        <property type="entry name" value="Zn_SUZ12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DUF7651" evidence="9">
        <location>
            <begin position="70"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="Polycomb protein SUZ12-like zinc finger" evidence="8">
        <location>
            <begin position="313"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="Polycomb protein VEFS-Box" evidence="7">
        <location>
            <begin position="502"/>
            <end position="608"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF09733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF23320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF24663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7752731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="634" mass="71801" checksum="C47F15DE588DE6D4" modified="2024-05-29" version="1">MPGIFIAAHETNYSVSSDQMCRNDARAHLTQEEQAAAEESLSVYCKPVELYNIIQRRAVRNPLFLQRCLHYKLQAKQKKRQESLHQIHNYLIQISVSVSGATSDGHQTQTLFPLYVLLARPVSTTNVETQSTNVYRFNRARKLTIFGGAETSSKGRAKFILPEMNKLSKEIKSGSLDVLLVSCADSTDSPKIDLTEDHMFSNTLNCAGYCLFGKIPMDLLHSSWEKSPTLSLGGRAEMMSTVIMQSCSMKLSSSNGEKCVSFRFPYNAEAVSILQQVPVVISAEEFGAKNMLPYDVYSYNDAPRPGIMRLRSGNVIFNYKYYNNTLHRTEVTEDFSCPFCLVKCASYKGLRFHLTSSHDLFHYEFWVTEDCQVVIVSMRTDVGSSEIIPENVDPKQQIFSYCHKSRRRRKPKSQTQNANHVHPLVLDSTMSTALSELIDNTDGVPESMELDACSPDASATCPSFAEPEPIQSVPESNLAPPALLQFAKTRKLSIERSDPRSRALLQKRQFFHSHRAQPMALEQVFAERDSEDEVDDDVADLEDRRMLDDFVDVSSDEKQMMHLWNSFVRKQRVLADGHIPWACEAFSKLHGPDLTQAPSLLWSVQHFLPFTSKVLEIVHDQIMESWAPRLAEPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D351</accession>
    <name>A0AAD5D351_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein ENHANCED DISEASE RESISTANCE 2 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_020339</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005683">
        <property type="protein sequence ID" value="KAI7752447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D351"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009769">
        <property type="entry name" value="EDR2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31558">
        <property type="entry name" value="CW14 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31558:SF16">
        <property type="entry name" value="FAMILY PROTEIN, PUTATIVE (DUF1336)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07059">
        <property type="entry name" value="EDR2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein ENHANCED DISEASE RESISTANCE 2 C-terminal" evidence="1">
        <location>
            <begin position="104"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7752447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="358" mass="41022" checksum="A70BED52776CD3C7" modified="2024-05-29" version="1">MRKKNKNLGNNVIVHSTSIGRDTSSISSPNLTQMDWHHSRFNSSSNVLFQEESWADSISKADESDSDDEFSETQEGISERNLVRPRAGFLIPCCTYKKLTPGCWSAIDPSSFTLRDENFFKHKTKSPAQSYCPYTPIGVDLFVSSRKVNHIAQLVELPSVKGHGMLPPLLIVNIQVPTYSAAMFNRDCDGDGLSLVLYFKLSETYEKDTSFQFQESIKRLMEDDMEKVRRFTKESFVSFRDRLKIIVGVMNPEDLVSNTTERKLLNAYNEKPVLSRPQHRFYQGSNYFEIDLDIHRFSYVARKGLESFRQRLKNGILNLGLTIQAQKPEELPEKMLCCLRLNKIDFVNHGQIPTIVAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D5I6</accession>
    <name>A0AAD5D5I6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Chlorophyll a-b binding protein, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">M8C21_011816</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">AA19_3_7</strain>
            <tissue evidence="21">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 20">The light-harvesting complex (LHC) functions as a light receptor, it captures and delivers excitation energy to photosystems with which it is closely associated.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">The LHC complex consists of chlorophyll a-b binding proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Plastid</location>
            <location evidence="2">Chloroplast thylakoid membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 20">Belongs to the light-harvesting chlorophyll a/b-binding (LHC) protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005399">
        <property type="protein sequence ID" value="KAI7753555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D5I6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009522">
        <property type="term" value="C:photosystem I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009523">
        <property type="term" value="C:photosystem II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016168">
        <property type="term" value="F:chlorophyll binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009765">
        <property type="term" value="P:photosynthesis, light harvesting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3460.10:FF:000001">
        <property type="entry name" value="Chlorophyll a-b binding protein, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3460.10">
        <property type="entry name" value="Chlorophyll a/b binding protein domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001344">
        <property type="entry name" value="Chloro_AB-bd_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022796">
        <property type="entry name" value="Chloroa_b-bind"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21649">
        <property type="entry name" value="CHLOROPHYLL A/B BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00504">
        <property type="entry name" value="Chloroa_b-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103511">
        <property type="entry name" value="Chlorophyll a-b binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 19 20" id="KW-0148">Chlorophyll</keyword>
    <keyword evidence="6 20" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="15 20" id="KW-0157">Chromophore</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7 20" id="KW-0602">Photosynthesis</keyword>
    <keyword evidence="12 20" id="KW-0603">Photosystem I</keyword>
    <keyword evidence="18 20" id="KW-0604">Photosystem II</keyword>
    <keyword evidence="9 20" id="KW-0934">Plastid</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="16 20" id="KW-0793">Thylakoid</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="binding site" description="axial binding residue" evidence="19">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>chlorophyll b</name>
            <dbReference type="ChEBI" id="CHEBI:61721"/>
            <label>1</label>
        </ligand>
        <ligandPart>
            <name>Mg</name>
            <dbReference type="ChEBI" id="CHEBI:25107"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>chlorophyll a</name>
            <dbReference type="ChEBI" id="CHEBI:58416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>chlorophyll a</name>
            <dbReference type="ChEBI" id="CHEBI:58416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>chlorophyll a</name>
            <dbReference type="ChEBI" id="CHEBI:58416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>chlorophyll a</name>
            <dbReference type="ChEBI" id="CHEBI:58416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="19">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>chlorophyll a</name>
            <dbReference type="ChEBI" id="CHEBI:58416"/>
            <label>1</label>
        </ligand>
        <ligandPart>
            <name>Mg</name>
            <dbReference type="ChEBI" id="CHEBI:25107"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>chlorophyll a</name>
            <dbReference type="ChEBI" id="CHEBI:58416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>chlorophyll a</name>
            <dbReference type="ChEBI" id="CHEBI:58416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="19">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>chlorophyll b</name>
            <dbReference type="ChEBI" id="CHEBI:61721"/>
            <label>1</label>
        </ligand>
        <ligandPart>
            <name>Mg</name>
            <dbReference type="ChEBI" id="CHEBI:25107"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="19">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>chlorophyll b</name>
            <dbReference type="ChEBI" id="CHEBI:61721"/>
            <label>1</label>
        </ligand>
        <ligandPart>
            <name>Mg</name>
            <dbReference type="ChEBI" id="CHEBI:25107"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="19">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>chlorophyll b</name>
            <dbReference type="ChEBI" id="CHEBI:61721"/>
            <label>1</label>
        </ligand>
        <ligandPart>
            <name>Mg</name>
            <dbReference type="ChEBI" id="CHEBI:25107"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="19">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>chlorophyll b</name>
            <dbReference type="ChEBI" id="CHEBI:61721"/>
            <label>3</label>
        </ligand>
        <ligandPart>
            <name>Mg</name>
            <dbReference type="ChEBI" id="CHEBI:25107"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>chlorophyll a</name>
            <dbReference type="ChEBI" id="CHEBI:58416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>chlorophyll a</name>
            <dbReference type="ChEBI" id="CHEBI:58416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>chlorophyll a</name>
            <dbReference type="ChEBI" id="CHEBI:58416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>chlorophyll a</name>
            <dbReference type="ChEBI" id="CHEBI:58416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>chlorophyll a</name>
            <dbReference type="ChEBI" id="CHEBI:58416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="240"/>
        </location>
        <ligand>
            <name>chlorophyll a</name>
            <dbReference type="ChEBI" id="CHEBI:58416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="19">
        <location>
            <position position="249"/>
        </location>
        <ligand>
            <name>chlorophyll a</name>
            <dbReference type="ChEBI" id="CHEBI:58416"/>
            <label>6</label>
        </ligand>
        <ligandPart>
            <name>Mg</name>
            <dbReference type="ChEBI" id="CHEBI:25107"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="19">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>chlorophyll b</name>
            <dbReference type="ChEBI" id="CHEBI:61721"/>
            <label>1</label>
        </ligand>
        <ligandPart>
            <name>Mg</name>
            <dbReference type="ChEBI" id="CHEBI:25107"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR601344-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU363080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAI7753555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="260" mass="27518" checksum="FEB3CA9360E308FB" modified="2024-05-29" version="1">MAASTMALSSAFAGQAAKLTPSGSELSGNGRVSMRKATVKQVSSGSPWYGPDRVKYLGPFSGEAPSYLTGEFPGDYGWDTAGLSADPEAFAKNRELEVIHCRWAMLGALGCLLARNGVKFGEAVWFKAGAQIFSEGGLEYLGNPSLIHAQSILAIWATQVILMGAVEGYRIAGGPLGEVVDPLYPGGSFDPLGLADDPEAFAELKVKEIKNGRLAMFSMFGFFVQAIVTGKGPLENLADHLADPVANNAWFYATNFVPGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D7N7</accession>
    <name>A0AAD5D7N7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Myb/SANT-like DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_024579</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010003072">
        <property type="protein sequence ID" value="KAI7754537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D7N7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000152">
        <property type="entry name" value="Trihelix transcription factor ASIL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044823">
        <property type="entry name" value="ASIL1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044822">
        <property type="entry name" value="Myb_DNA-bind_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31307:SF7">
        <property type="entry name" value="SEQUENCE-SPECIFIC DNA BINDING TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31307">
        <property type="entry name" value="TRIHELIX TRANSCRIPTION FACTOR ASIL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13837">
        <property type="entry name" value="Myb_DNA-bind_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb/SANT-like DNA-binding" evidence="2">
        <location>
            <begin position="49"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="261"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="269"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7754537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="363" mass="40218" checksum="2E632DF94998A463" modified="2024-05-29" version="1">MDNNNNNNNNNNNNKSIKQENHHDHSSPKKHHLMASGSGAGPGADRMKRDEWSEGAVSSLLEAYEAKWTLRNRAKLKGHDWEDVAKHVSSRAGSSKSPKTQTQCKNKIESMKKRYRSESATADVSSWPLYSRLDLLLRGSGGGGGGGGGSAPVRTDPVPVSSNMNMIVFDQAPTASIPPSMTTTAMMIESSAVPVSVAVPAPVTATVNEGIEQNSNDSNGVEPENKEEIFVTKMPENPHSEKMEIETDCSTPALYSNEKDKEKIETKNHKTRVRESRKKRRRGENWDVAESIRWLAKVMMRSEQAKVETMREVEKMRADAEVKRGELDLKRTEIIAHTQLEIARLFATCLGKAVDPSLRIGRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DA42</accession>
    <name>A0AAD5DA42_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 9">Malate dehydrogenase</fullName>
            <ecNumber evidence="2 9">1.1.1.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_025602</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>(S)-malate + NAD(+) = oxaloacetate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21432"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.37"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the LDH/MDH superfamily. MDH type 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.37" evidence="2 9"/>
    <dbReference type="EMBL" id="JAMZMK010003837">
        <property type="protein sequence ID" value="KAI7754370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DA42"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030060">
        <property type="term" value="F:L-malate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006108">
        <property type="term" value="P:malate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000010">
        <property type="entry name" value="Malate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.110.10">
        <property type="entry name" value="Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001557">
        <property type="entry name" value="L-lactate/malate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022383">
        <property type="entry name" value="Lactate/malate_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001236">
        <property type="entry name" value="Lactate/malate_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015955">
        <property type="entry name" value="Lactate_DH/Glyco_Ohase_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001252">
        <property type="entry name" value="Malate_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010945">
        <property type="entry name" value="Malate_DH_type2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003916">
        <property type="entry name" value="PRK05442.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23382">
        <property type="entry name" value="MALATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02866">
        <property type="entry name" value="Ldh_1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00056">
        <property type="entry name" value="Ldh_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000102">
        <property type="entry name" value="Lac_mal_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56327">
        <property type="entry name" value="LDH C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00068">
        <property type="entry name" value="MDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7 9" id="KW-0520">NAD</keyword>
    <keyword evidence="3 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Lactate/malate dehydrogenase N-terminal" evidence="10">
        <location>
            <begin position="7"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="Lactate/malate dehydrogenase C-terminal" evidence="11">
        <location>
            <begin position="157"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="188"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="12"/>
            <end position="18"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="130"/>
            <end position="132"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7754370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="268" mass="28766" checksum="1A67F9950A6F6F4C" modified="2024-05-29" version="1">MAKDPIRVLVTGAAGQIGYALVPMIARGIMLGPDQPVILHMLDIPPAAEALNGVKMELVDAAFPLLKGVVATTDAVEACTGVNVAVMVGGFPRKEGMERKDVMSKNVSIYKSQASALEKYAAANCKVLVVANPANTNALILKEFAPSIPEKNITCLTRLDHNRALGQISEKLNVQVSDVKNVIIWGNHSSTQYPDVTHATGTWVSMGVYSDGSYDVPAGLIYSFPVTCRNGEWTIVQGLSIDEFSRKKLDLTAEELSEEKALAYSCLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DAW9</accession>
    <name>A0AAD5DAW9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_010138</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010001183">
        <property type="protein sequence ID" value="KAI7755391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DAW9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7755391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="23" mass="2550" checksum="7B067F77D077FAB4" modified="2024-05-29" version="1">MLSSLLTISRLTEKKIVSSSGIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DB96</accession>
    <name>A0AAD5DB96_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">NAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_000478</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000126">
        <property type="protein sequence ID" value="KAI7757428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DB96"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.150.80:FF:000002">
        <property type="entry name" value="Nac domain-containing protein 86"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.80">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003441">
        <property type="entry name" value="NAC-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036093">
        <property type="entry name" value="NAC_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31744:SF210">
        <property type="entry name" value="NAC DOMAIN-CONTAINING PROTEIN 86-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31744">
        <property type="entry name" value="PROTEIN CUP-SHAPED COTYLEDON 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02365">
        <property type="entry name" value="NAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101941">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51005">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NAC" evidence="7">
        <location>
            <begin position="15"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="190"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="222"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="256"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7757428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="489" mass="55029" checksum="AD3ADA691E054703" modified="2024-05-29" version="1">MNHEPILKPPPKSSLAPGFRFHPTDEELVRYYLRRKVCRKPFRFHSLSETDVYKSEPWELADYSQLNSRDQEWYFFSPVDKKYGNGSRLNRATGQGYWKATGKDRAVHHKGQAIGSKKTLVFHVGRAPDGKRTNWVMHEYRLSDQELESAQVAQDAFVLCRIFEKSGLGPPTRDRYAPFLEEEWEDDEALFVPGGEPDEDTTNGDETRNDTVKTGTQTIDFVSKRARSEDDDGLKSETDVDPLSLLNKKSKQVDPSSSNANGSDDSATTSQDPKTTNLSSALVELSLLEPVAAKEAHPTTPPSFDASTLEKSVPPAYLKYISNLEARIRTVTTEKENLKVEVMRSQAMINVLHTRIDQLTKENKEIKSGVVNHATDEHKRPIDQWSCCVEEARHKFLWENEEAGASATNNEETNFVAIWNSFFCLDLSQSRSTANISITRGNALLAASVSRKARRDLLAMKSTADSQYSFRAQYATSASRIPTVSAHKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DBC1</accession>
    <name>A0AAD5DBC1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_030938</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000450">
        <property type="protein sequence ID" value="KAI7756330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DBC1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044806">
        <property type="entry name" value="WVD2/WDL1-4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46372:SF6">
        <property type="entry name" value="PROTEIN WVD2-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46372">
        <property type="entry name" value="PROTEIN WVD2-LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="151"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="163"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7756330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="172" mass="18644" checksum="CE76EE24EC6AB7BC" modified="2024-05-29" version="1">MGRDTDSLRVDKKPNTSKVNSNGVSSGTNHTSPKPSGESVNIRKSEAEEGPQVEPTYENQEAVSPKPEPSKSSEKIINSLEHNGTAGLHMSSNNSGFRSPINTKQSQHSPFSTPRTAKSFTDKYHDEEDNWSLASSAATSRTVKSRVTVGVAPSFKSAQRAAQRKEVISYLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DC28</accession>
    <name>A0AAD5DC28_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_022968</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000303">
        <property type="protein sequence ID" value="KAI7756690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DC28"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022801">
        <property type="entry name" value="Ribosomal_uS4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831">
        <property type="entry name" value="30S 40S RIBOSOMAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831:SF47">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US4Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="22"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="81"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7756690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="107" mass="12053" checksum="40C61AF4EA101CAE" modified="2024-05-29" version="1">MAKSIHHARVLIKQRHIRYNKLVAYTLFARLLVTSLFILLFGLALYRVGRQVVNVPSFMVRIDSQKHIDFSLTSPFGGCQPGRVKRKNQKAAAKNAVGGDADEDDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DCX1</accession>
    <name>A0AAD5DCX1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_013734</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000005">
        <property type="protein sequence ID" value="KAI7758373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DCX1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008964">
        <property type="term" value="F:phosphoenolpyruvate carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015977">
        <property type="term" value="P:carbon fixation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048366">
        <property type="term" value="P:leaf development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021135">
        <property type="entry name" value="PEP_COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001313">
        <property type="entry name" value="Pumilio_RNA-bd_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30523">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30523:SF47">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE CARBOXYLASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00311">
        <property type="entry name" value="PEPcase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00806">
        <property type="entry name" value="PUF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00150">
        <property type="entry name" value="PEPCARBXLASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00025">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50302">
        <property type="entry name" value="PUM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0810">Translation regulation</keyword>
    <feature type="repeat" description="Pumilio" evidence="4">
        <location>
            <begin position="200"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="4">
        <location>
            <begin position="237"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="4">
        <location>
            <begin position="280"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7758373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="721" mass="80760" checksum="EF4BAE25E575F737" modified="2024-05-29" version="1">MESPFKPEILKGKVALLTGGASGIGFEISTQFSKHGASIAVMGRCKTVVDSAVSSLQSLGIPVLTFCLFWLTCDHASLQEWGTDYDIRVNGIAPGPINDTAGVRKLGPDEIKSKTMEMMPLFKLGEKWDIAVAALYLFYSSSIRLHLFSIDRSHFLLRLTVPLDATTGNQTRVPLGEAAWTFGVVDPNSHTAALLEFLFEAATKKCMELAIDRHGCCVLQKCLSHSDDEPRRRLVREITSNALILSQDPYGNYVVQDVFELQVPWATSAILDQLEGNYGDLAMQKYSNQIMQDPYGNYVIQAGILSSKVALREALVKAIKPHVPTLRTSSYAKKIHPNEPYRIILGDVRDKLNHTRERSRQLLANGVFDVPENMTFTTVEQFLEPLELCYKSLCAFGDRPIGDGSLLDNFRQVSTFRLSLISLDICQESDRHTDVIDSITKYLEIGSYRSWSEEQRQEWLLAQLKGKRPLFGHDRPETEEMSDVLDTLRLISELPSDNFGAYIISMATSPSDVLVDDLLQRECQVKSPLRVVPLFEKLADLKAAPTAMARLFSIECYKNRINGKHEVMIGYSDYGKDAAAFKHAIAKDPKNLEMRVMYNEWPFFRVTIDLVEMVFAKGNPGIAASYDKLLVSRELLAFGERLRANYEETKNLLLLAYTLKWIHSPSSDVKVRPHLSKDIMESTKPAAELVKLNPTSDYVPGLEDALILTIKDIVAGMQNTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G1V4</accession>
    <name>A0AAD5G1V4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">VTT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_015464</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012184">
        <property type="protein sequence ID" value="KAI7724603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G1V4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032816">
        <property type="entry name" value="VTT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47699">
        <property type="entry name" value="SNARE ASSOCIATED GOLGI PROTEIN FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47699:SF1">
        <property type="entry name" value="SNARE ASSOCIATED GOLGI PROTEIN FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09335">
        <property type="entry name" value="VTT_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="146"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="176"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="214"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="VTT" evidence="2">
        <location>
            <begin position="75"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7724603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="252" mass="27690" checksum="B7D9F94314F03A1B" modified="2024-05-29" version="1">MVSGRKLGLFLTVVVVIAVVTLFKIKRSSSSSPSPSAWDKESLVEFFKHMSDRLGNWAIPVYVAIHTVSLALCLPYAVFFEAGASLLFGFFPALVCVFSAKVLGASLSFWIGRFVFKSSSSATRWAHENKYFHVLSRGVERDGWKFVLLARFSPIPSYIINYALAATNVRFLKDFLAPTVLGCLPMILQNTSIGSLAGAAVATSAGSQSNKSNIWSYVFPMMGISSSILISWRIKKYSSSISVDDKTVEKTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G365</accession>
    <name>A0AAD5G365_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">peroxidase</fullName>
            <ecNumber evidence="3">1.11.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">M8C21_008086</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">AA19_3_7</strain>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 18">
            <text>2 a phenolic donor + H2O2 = 2 a phenolic radical donor + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:56136"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:139520"/>
            <dbReference type="ChEBI" id="CHEBI:139521"/>
            <dbReference type="EC" id="1.11.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="11">Binds 2 calcium ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the peroxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.7" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010011435">
        <property type="protein sequence ID" value="KAI7727134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G365"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140825">
        <property type="term" value="F:lactoperoxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002016">
        <property type="entry name" value="Haem_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000823">
        <property type="entry name" value="Peroxidase_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019794">
        <property type="entry name" value="Peroxidases_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31388:SF115">
        <property type="entry name" value="PEROXIDASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31388">
        <property type="entry name" value="PEROXIDASE 72-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00141">
        <property type="entry name" value="peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00461">
        <property type="entry name" value="PLPEROXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00436">
        <property type="entry name" value="PEROXIDASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50873">
        <property type="entry name" value="PEROXIDASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0349">Heme</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="6 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042262222" description="peroxidase" evidence="14">
        <location>
            <begin position="24"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="Plant heme peroxidase family profile" evidence="15">
        <location>
            <begin position="24"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="12">
        <location>
            <position position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAI7727134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="106" mass="11824" checksum="0DE8A4B007B631AD" modified="2024-05-29" version="1">MASTNIIAGIFCLLITINTSSEAQLSSTFYDGICPNALRTIKTTIRTAISRERRMAASIIRLHFHDCFVQVLVVQSHVTKKTKISNKNCRKTSDGIQRPSLDGREI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G3P5</accession>
    <name>A0AAD5G3P5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pentatricopeptide repeat-containing protein At5g55840</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_003450</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011339">
        <property type="protein sequence ID" value="KAI7727659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G3P5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45613:SF9">
        <property type="entry name" value="MITOCHONDRIAL GROUP I INTRON SPLICING FACTOR CCM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45613">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12854">
        <property type="entry name" value="PPR_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="20"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="36"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="105"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="140"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="175"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="210"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="245"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="280"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="315"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="350"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="385"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="420"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="455"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="525"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="596"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="631"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="666"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="701"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="841"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="876"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="911"/>
            <end position="945"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7727659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1014" mass="114263" checksum="11774C6B15D85BEC" modified="2024-05-29" version="1">MYANANSILTRLSDMGIGANSVFSALMKTYPLCKSNPAVFDLLIRGYLRESSVESALEVFRLMGIRGFNPSVYTCNMVLSALGKESYTTAWLFFKEMVSLNVCPNVATFNILVNILCSNGNVTKARHLVWKMEESGCVPNLVTYNTLLKWYFKKGRYKAALELIDHMGHKGLEADVCTYNMFIDKLCQNNRSSKGYLLLKKMRKKPLSPNEVTYNTLIKGFIKEGKLSVALYVFDEMLKVRISPNIVTYNSLIDGHCRENSFKEATELLNEMEARGLRPNEVSYGVILNGLCKLGELDLAFSLVESMRMNGITVDHVSYTMLVKGFIKSRRLKEGVQVLDKMIQNGVDADVVTYAVLINGFCREGKFRTAKELMSKMYKSGVTPNKVMYSTLVYDYCNHGKVNEAMRIYKIMNFSGLDVDLFVCNKLVDSLCKSQRVEEAVEFLKHMGRIGISPDTSTFNFIISCYVSIGNGLKAFDCFEEMVKFGQRPSSHTYGSLLKALSYGGNFEEARKFLNKIRSIPYALDAVSYNMLMAEACKSGNLQDAVFLLKDMVRNSVIPDSHTYTCLLASLCKNEKMVVSILLSEKWMEKGALLPNVVTYTCLINGLFRAGQPKTAAFYHQKMMSEGLSADTILLNVLMDGYSRMGNMSMVYNLFSKMSDGVLSPNLATYNILLHGYSKQDDISRCFKVYGALLRNGFVPDKRTCHALIYGICKAGMLDFASKMLKSMILKGMLPDKHTLDMVILKCSERRKIVKAFEFLDLMNMLRISPDQDTFNIILKGIKETYGCQPSNLFLNEMLEKGFIPSEKQVVRLITDMCKVGDVKGANKLKNDIKSYGVISGNVAESALVRGLAQRGKLEEALLVLDCMLRGKIVPTTATFTTLIHSLCKKLEFEEALKLKRIMEIHNVRLDVVTYNVLITGFCENGDLVEALKLYKEMKKRGMCPQVNTFCVIIESFCNKNDFPDGEMILNDLCERGLLAEDEVTQDIANSFNVALKKLNILKHKRNRTCFRNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G425</accession>
    <name>A0AAD5G425_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transducin/WD40 repeat-like superfamily protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_020427</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011384">
        <property type="protein sequence ID" value="KAI7727422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G425"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22857">
        <property type="entry name" value="WDR74"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001732">
        <property type="entry name" value="WD repeat-containing protein 74"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037379">
        <property type="entry name" value="WDR74/Nsa1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16038">
        <property type="entry name" value="NOP SEVEN ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16038:SF4">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 74"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="298"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="360"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="372"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="388"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="420"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="453"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7727422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="485" mass="53379" checksum="6B03FACCC599374B" modified="2024-05-29" version="1">MPRTTTLETPGCPPLRAITFDVLGLIKVTEARSKERGVPKVVERWGEPDASRCVLAASINDRKADPLLAVARKTGVVELLSPINGDLRVSLATTNQTDAQPQEDNIVGLHLFKKQPSESSSRSCTLLTCTTKGVTTMRSVMIPKALGDSTGDDTPTTWNICAAGNVLCCKVDESENYALFGGKGVEVNIWDLATHTKIWTAKSPPKNSLGIFTPTWFTSATFLSKDDHRKFAAGTNSHQVRLYDMSAQRRPVMSFDFRETPIKAVTEDEDGNTIYIGNGSGDLASVDIRTGKLLGCFLGKCSGSIRSITRHPDLPVIASCGLDSYLRIWDIKSRQLLSAVFLKQHLTNVVFDSNFEDEEVAGSTAPLPPSEQEIDDEPVDRDEESPRVKQKKKKRSKSRSLDENEEVAVSSKHKKLKKSRNLDGEDEGPRSAKQKKAKKSQNVDEDEELISVEQEKSKKSKVKSKDGKSKKSKKSKKSKAESIDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G5T6</accession>
    <name>A0AAD5G5T6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_018132</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010916">
        <property type="protein sequence ID" value="KAI7729804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G5T6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010597">
        <property type="term" value="P:green leaf volatile biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000762">
        <property type="term" value="P:regulation of phenylpropanoid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000011">
        <property type="entry name" value="Myb transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000358">
        <property type="entry name" value="Myb-related protein 305"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044676">
        <property type="entry name" value="EOBI/EOBII-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45675">
        <property type="entry name" value="MYB TRANSCRIPTION FACTOR-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45675:SF44">
        <property type="entry name" value="TRANSCRIPTION FACTOR MYB24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="8">
        <location>
            <begin position="11"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="7">
        <location>
            <begin position="11"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="8">
        <location>
            <begin position="64"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="7">
        <location>
            <begin position="64"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7729804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="190" mass="22038" checksum="B76A9F3AC81EA13A" modified="2024-05-29" version="1">MEKKPCNSSQDVEVRKGPWTMEEDLILINYIANHGEGVWNSLARSAGLKRTGKSCRLRWLNYLRPDVRRGNITPDEQLLIMELHAKWGNRWSKIAKHLPGRTDNEIKNYWRTRIQKHIKQAENFSARTSSGHTDQATASSTQTCNAINPMDTYSPPYENFQNFSSSFPTETNENMWSMEDLWSMQLLNSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G6U8</accession>
    <name>A0AAD5G6U8_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_017494</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010659">
        <property type="protein sequence ID" value="KAI7730749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G6U8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7730749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="71" mass="8701" checksum="7F3FF27D25995334" modified="2024-05-29" version="1" fragment="single">MYIWPFGQFTIFTLGKSLLQQRKTMDEKFVCLRQQHPLKHQMCRPTMRSQMSFLNLLLRIIIAFWLQKRKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G7I1</accession>
    <name>A0AAD5G7I1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_011520</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010453">
        <property type="protein sequence ID" value="KAI7731409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G7I1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7731409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="117" mass="12824" checksum="45D21E65DD2F2A9B" modified="2024-05-29" version="1">MSAIEVKMRSKMIVYLFLIVLCNHVILSSADSNNPSLQQELHGVPALTGLYSNKNDPEGSKYILKQGQKGRRGGAHGGGGSANMDRSRHGPRNTVQSKHAKSFSVIYVSFFLLLFAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G7S2</accession>
    <name>A0AAD5G7S2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_005784</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010727">
        <property type="protein sequence ID" value="KAI7730571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G7S2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042252630" evidence="1">
        <location>
            <begin position="23"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7730571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="80" mass="9053" checksum="B95041D06A85591D" modified="2024-05-29" version="1" fragment="single">MKKLLFFSFSLILYSNVAFIQSANLHTQEVKVLKQIWGKLGILGKEWDFDKDPCSGEGKWGTSVLCDCSFDSNTTCRVTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G8J6</accession>
    <name>A0AAD5G8J6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein DETOXIFICATION</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Multidrug and toxic compound extrusion protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_012515</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010213">
        <property type="protein sequence ID" value="KAI7732459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G8J6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13132">
        <property type="entry name" value="MATE_eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045069">
        <property type="entry name" value="MATE_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11206">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="54"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="87"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="136"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="202"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="238"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="268"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="351"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="395"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="424"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="454"/>
            <end position="474"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7732459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="490" mass="53441" checksum="3AA5D4445A15FE49" modified="2024-05-29" version="1">MAINGTGGRVDEHEPTLVPLLDHSSSNNDLTSKTTDQELELSFQQRFLIESKKLWHIVGPAIFSRIASYSMFVITQAFAGHLGDLELAAISIATSVIVGFDFGLLLGMASALETLCGQAYGAKNYRMLGVYLQRSWIVLFVCCIALLPLYIFASPVLKLLGQPADIAELSGLVSMSLIPLHFSLCFQFPLQRFLQSQLKNYVIAWVSLVALVVHSAMSWLVVARFQLGLVGTVVTLNISWWLIVLGLFVYVVFGGCPESWGGFSMEAFSGLWAFVKLSVASGIMLCLENWYYRILIVMTGNLENAKIAVDALSICMSINGFELMIPLGFFAGTGVRVANELGAGNGKGARFATIVAVTTSTMIGVVFWILIMFFHNELALIFTNSQPVLDAVSQLSLLLAFTILLNSVQPVLSGVAVGSGWQSYVAYINLGCYYLIGVPMGLAMGWVFHLGVMGIWAGMIFGGTAFQTVVLAIITSRCNWELEVSYFKLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G8W5</accession>
    <name>A0AAD5G8W5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Importin subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_033720</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the importin alpha family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009970">
        <property type="protein sequence ID" value="KAI7733480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G8W5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061608">
        <property type="term" value="F:nuclear import signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.690:FF:000002">
        <property type="entry name" value="Importin subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000040">
        <property type="entry name" value="Importin subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.690">
        <property type="entry name" value="Importin-alpha, importin-beta-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032413">
        <property type="entry name" value="Arm_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002652">
        <property type="entry name" value="Importin-a_IBB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036975">
        <property type="entry name" value="Importin-a_IBB_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024931">
        <property type="entry name" value="Importin_alpha"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23316">
        <property type="entry name" value="IMPORTIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00514">
        <property type="entry name" value="Arm"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16186">
        <property type="entry name" value="Arm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01749">
        <property type="entry name" value="IBB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005673">
        <property type="entry name" value="Importin_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51214">
        <property type="entry name" value="IBB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5 7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="IBB" evidence="8">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="6">
        <location>
            <begin position="116"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="6">
        <location>
            <begin position="159"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="6">
        <location>
            <begin position="327"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR005673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7733480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="531" mass="58853" checksum="338C9C5C233EBCE2" modified="2024-05-29" version="1">MSLRPSDRTDLRRNRYKVAVDADEGRRRREDNMVEIRKNRREESLQKKRREGLAANQFPVPVQSASVGEKKLENLPAMVAGLYSDDSSMQLEATTQFRKLLSIERSPPIEEVIKTGVVPRFVQFLMREEYAQLQFESAWALTNIASGTSEHTKVVIDHGAVPIFVKLLASSNDDVREQAVWALGNVAGDSPKCRDLVLAEGALQSLLLLLNANAKLSMLRNATWTLSNFCRGKPQPAFEQTKPALPALQQLIHSNDEEVLTDACWALSYLSDGTNDKIQAVIESNVCPRLVELLNHPSPTVLIPALRTVGNIVTGDDMQTQYIIDHQALPCLLNLLTNNHKKSIKKEACWTISNITAGNKEQIQTVIAANLIGPLVQLLQSAEFDIKKEAAWAISNATSGGSHDQIKYLVSQGCIKPLCDLLVCPDPRIVTVCLEGLENILKVGESEKNLGRSGDTNLYAQMIDDAEGLEKIENLQSHDNNEIYEKAVKLLETYWVEEEEDDVMPPGEIAQPDFNFGGGDVTVPNGGFNFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GAS2</accession>
    <name>A0AAD5GAS2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_028589</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Plastid</location>
            <location evidence="2">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the major facilitator superfamily. Phosphate:H(+) symporter (TC 2.A.1.9) family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the major facilitator superfamily. Sodium/anion cotransporter (TC 2.A.1.14) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009933">
        <property type="protein sequence ID" value="KAI7733646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GAS2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042170">
        <property type="term" value="C:plastid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17380">
        <property type="entry name" value="MFS_SLC17A9_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000058">
        <property type="entry name" value="ascorbate transporter, chloroplastic isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000086">
        <property type="entry name" value="ascorbate transporter, chloroplastic isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050382">
        <property type="entry name" value="MFS_Na/Anion_cotransporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044777">
        <property type="entry name" value="SLC17A9-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11662:SF255">
        <property type="entry name" value="ASCORBATE TRANSPORTER, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11662">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0934">Plastid</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="199"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="259"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="286"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="351"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="392"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="425"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="479"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="12">
        <location>
            <begin position="110"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="508"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7733646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="508" mass="55692" checksum="31619E1358B6DDC6" modified="2024-05-29" version="1" fragment="single">MAIGAVISHRNFTSLISSSGKIYQPEQVTAHQRAENLSFSSAQYVHRNTYSENMYSLKKIYWNTISSHGLDPVASNMLRSKAMILYDGENKKFHMTNHRQRAKGICRCFVSLPSESYNWIKPVQLKTISISNNQTLQPRVSSEGGASEVNMSIAILPMSKEFNWNSATVGLIQSSFFWGYLLTQIVGGIWADKIGGKQVLGFGVVWWSIATVLTPIAAKIGLPFLLVMRAFMGIGEGVAMPAMNNILSKWIPVSERSRSLALVYSGMYLGSVIGLAFSPILIQKFAWPSVFYSFGSLGSLWFAFWLSKAHSSPGDDPEVSAEEKEYILGGSISKEPVTSIPWKLILSKAPVWALIVSHFCHNWGTFILLTWMPTYYNQVLKFNLTESGLLCVLPWLTMAVFANIGGWIADTLVSRGVSVTNVRKIMQSIGFLGPAFFLTQLSSVKTPAMAVLCMACSQGSDAFSQSGLYSNHQDIGPRYAGVLLGLSNTAGVLAGVFGTAATGYILQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GAY6</accession>
    <name>A0AAD5GAY6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Replication protein A 70 kDa DNA-binding subunit B/D first OB fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_023796</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009968">
        <property type="protein sequence ID" value="KAI7733498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GAY6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd04481">
        <property type="entry name" value="RPA1_DBD_B_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003871">
        <property type="entry name" value="RFA1B/D_OB_1st"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47165">
        <property type="entry name" value="OS03G0429900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47165:SF4">
        <property type="entry name" value="OS03G0429900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02721">
        <property type="entry name" value="DUF223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Replication protein A 70 kDa DNA-binding subunit B/D first OB fold" evidence="2">
        <location>
            <begin position="8"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="370"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="370"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7733498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="423" mass="48555" checksum="F014E3A1A6FA0BE1" modified="2024-05-29" version="1">MATNAIVFIKDVKARKRNTSLRVRVANLWRKKMNGAENKTYRLDMILMDEMGDFIYANCMHTMLERFDSMLHIDETLYINNPFISKDDTKQHVDYDKKMSLNDKTTIIKCQNWEGPKFAFNFLCFKTLNQKTMKHNTKTDFIGYVKVCYNLDFVKKKNGIKAPIIKFKLRNLEGIEIDGSLWEDKAIALSSFMSNPDRDPHVFLLVQFASVNNYQGNMSVKNSYEASRVFINEDIEELRTFKQQYLAKFVDKESSESSNSTVGSYMISNETKVIIVGKISEIGTDLQWYYKGCNYFRAKVEKKFVSYEKDDGTVGGKEVKARFLVNRPAVDEVADINEDPVAQLEEVIEQDTMSSTNADESEMSRMKKIVAEDESGSSKRPVDDVYEVDGPTATSITKRKKVTAKEGGTMNNKMNLLIPKLEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GFA2</accession>
    <name>A0AAD5GFA2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_015146</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008920">
        <property type="protein sequence ID" value="KAI7737953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GFA2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14798">
        <property type="entry name" value="RX-CC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000322">
        <property type="entry name" value="Probable disease resistance protein At1g63360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056789">
        <property type="entry name" value="LRR_R13L1-DRL21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038005">
        <property type="entry name" value="RX-like_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766:SF47">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766">
        <property type="entry name" value="PLANT BROAD-SPECTRUM MILDEW RESISTANCE PROTEIN RPW8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25019">
        <property type="entry name" value="LRR_R13L1-DRL21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0611">Plant defense</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="9">
        <location>
            <begin position="13"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="8">
        <location>
            <begin position="200"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="10">
        <location>
            <begin position="444"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="domain" description="R13L1/DRL21-like LRR repeat region" evidence="11">
        <location>
            <begin position="689"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="891"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="911"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="929"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF25019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7737953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="929" mass="105871" checksum="8D40C74DBC49E4B9" modified="2024-05-29" version="1" fragment="single">ILIMGDAAVSALVKEVVGRITSELIKEFRLFRGFQKDILTLKNEFLLNEVVLEDADEKQNKDKAVQVWLKSLKSATMEAENVLDEVSTQALLQRLQKKRGRKYRVRAFFSSDHNTHMFRVRIAHKVKAIRAKLDDIASKRLKLGLNPGATSHVDEDSAGEMPNRETSSLVIRDSASIFGRDEEREMVVENICNSGIGKYENGEILVYGIWGMGGIGKTTLAKLVYDHEKVKQNFPLRCWVYVSEKFEVNEIIKKIIESIDRGECKLTQLDTLQVEVQGRLTGKKFLIVLDDVWIEDKEKAKWDELSKTLSCGEEGSIVVMTTRSSKTSQMMAKVDELQHKLGCLSKVDSWLLFSKFAFAQGRKGDAISVLEPIGREIVEKCSGLPLALKTLGSLMWSKKSTSDWEHVKGSIISKLQENNVLSVLKLSYDNLPLHFKRCLAYCCLFPKGYEMRKDELIQLWTANGFIPPKEDGEEILNCLVWRSFFQLVDTESYYENDPYEYKMHDLMHDMALHVMGDNCLVIESTGDKIPNEVFHLSSSCPEFVFSPDDLRNLASLRSILFFGKRNESRLSQIVNHMYLRVLYLCNLRLETLPESIGKLKHLRYLNLSNSRIRVLPESIIHLQNLQMLILFCCNFLVELPKGLRHMVNFQSLDIGGCYALRRLPVGIKELTRLRILSKFPLVKEGGAKIGELGDLVLLEGELEIEGLENVEGFSEAKSANLKCKRNLSVLKLSWTYAKKVGGWYKCDVIKEVLEGLEPNPGLKVLKISNYKDKIISPSWMVNLKNLIKISFSSCEKFKHIPPLGRLPNLRVIEMENMDCLGFHEDDTNMSGDNPIIFPSLQTLHISYCFRLVSLPSNLPKLRELRYKHEMGEDWPKVSHIPDVEIFFFESSDSKDEGDVDNDEFVPAPSSDSEDEDEDDVDNVEFVPAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GFS5</accession>
    <name>A0AAD5GFS5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cyclin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_006502</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclin family. Cyclin AB subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008329">
        <property type="protein sequence ID" value="KAI7740860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GFS5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044772">
        <property type="term" value="P:mitotic cell cycle phase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20562">
        <property type="entry name" value="CYCLIN_AtCycA_like_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000167">
        <property type="entry name" value="Mitotic cyclin 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039361">
        <property type="entry name" value="Cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046965">
        <property type="entry name" value="Cyclin_A/B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004367">
        <property type="entry name" value="Cyclin_C-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048258">
        <property type="entry name" value="Cyclins_cyclin-box"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10177">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02984">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001771">
        <property type="entry name" value="Cyclin_A_B_D_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00292">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="3 5" id="KW-0195">Cyclin</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="7">
        <location>
            <begin position="237"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="104"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="36"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="108"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7740860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="414" mass="46354" checksum="EC14AF7195E27514" modified="2024-05-29" version="1">MSTVSNNRKLNGTTSSLAKRQAAPPLMAKKRPALANVTNQRPAARNGSNLMVPKKGSSVTNSFGISSASVHLNPSAAVSKITSITRKDATVSKINIVPPVQTKMEVSPSKSDGLSVSMDETMSTCDSLNSPDVEYIDSNDIAAIESIERKTSSKLNISDNVEPTANVCKREILVEIGSNDKIVDLDVDFMDPQLCATMACDIYQHLRASEVKKRPAVDFMEKVQKDINASMRAILIDWLVEVAEEYRLVPDTLYLTINYIDRYLSGNLMDRQRLQLLGVACMMIASKYEEICAPQVEEFCYITDNTYFKDEVLQMESSVLNFLKFELTAPTVRCFLRRFVRAAHGVNENSTLKHYTQYQPSELFECVKSLHTLVTECPNSSLPAIREKYSQHKYKFVAKKHCPASIPMEYFLNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GGC0</accession>
    <name>A0AAD5GGC0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_010455</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008688">
        <property type="protein sequence ID" value="KAI7738988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GGC0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000050">
        <property type="entry name" value="Calcium-dependent protein kinase 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="171"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="207"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="243"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="278"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7738988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="351" mass="40214" checksum="6C50B3EA5BC39482" modified="2024-05-29" version="1" fragment="single">TPDSKHYQPPPTPQDKKRPTNHNNKQRRPNPYAETPVPIKVLTDFSQLTTHRTRISDKYILGRELGRGEFGVTYLCTDRETKQAFACKSISKKKLRTAVDVEDVRREVAIMSTVWKIWSRCVAEHPWIQNEKKASNVPLGDIVRTRLKQFSVMNRFKKKALRVIAEHLSLEEVEVIKDMFTLMDSDGDGKVTFDELKAGLKKVGSQLAEPEIRLLMDVADVDGNGVLEYGEFVAVTIHLQKMENDEHLRRAFMFFDRDGSGFIELDELEQVLYEPGQADIQVLNEIMKEVDTDKDGQISFEEFVAMMKAGTDWRKASRQYSRERFKSLSVNLMKDGSLRLEDGLTGQSVVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GI50</accession>
    <name>A0AAD5GI50_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C-CAP/cofactor C-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_003538</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CAP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008069">
        <property type="protein sequence ID" value="KAI7742134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GI50"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008179">
        <property type="term" value="F:adenylate cyclase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019933">
        <property type="term" value="P:cAMP-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.70:FF:000006">
        <property type="entry name" value="Adenylyl cyclase-associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001837">
        <property type="entry name" value="Adenylate_cyclase-assoc_CAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013912">
        <property type="entry name" value="Adenylate_cyclase-assoc_CAP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017901">
        <property type="entry name" value="C-CAP_CF_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016098">
        <property type="entry name" value="CAP/MinC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036223">
        <property type="entry name" value="CAP_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006599">
        <property type="entry name" value="CARP_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10652">
        <property type="entry name" value="ADENYLYL CYCLASE-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10652:SF0">
        <property type="entry name" value="ADENYLYL CYCLASE-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08603">
        <property type="entry name" value="CAP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00673">
        <property type="entry name" value="CARP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69340">
        <property type="entry name" value="C-terminal domain of adenylylcyclase associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51329">
        <property type="entry name" value="C_CAP_COFACTOR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C-CAP/cofactor C-like" evidence="2">
        <location>
            <begin position="1"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7742134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="154" mass="16615" checksum="3321B64C06B82AB5" modified="2024-05-29" version="1">MMYRWVVENQIGKKDLLIDKCDSKQTVYVFGCKDLVLQIKGKVNNITVDKCTKMGVVFTDVVAACEIVNCNSVEVQCQGTAPTISVDNSAGCQLYLSKGSLDASITTAKSSEINVLVPAAGPDNDLVEHALPQQYIHTYKNGQFVTTPVSHSGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GJ37</accession>
    <name>A0AAD5GJ37_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Nuclear pore complex protein NUP98A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_032699</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleoporin GLFG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007835">
        <property type="protein sequence ID" value="KAI7743034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GJ37"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044614">
        <property type="term" value="C:nuclear pore cytoplasmic filaments"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008139">
        <property type="term" value="F:nuclear localization sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000973">
        <property type="term" value="P:post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006405">
        <property type="term" value="P:RNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034398">
        <property type="term" value="P:telomere tethering at nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.2360:FF:000001">
        <property type="entry name" value="Nuclear pore complex protein Nup98-Nup96"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2360">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037665">
        <property type="entry name" value="Nucleoporin_S59-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23198">
        <property type="entry name" value="NUCLEOPORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23198:SF32">
        <property type="entry name" value="NUCLEOPORIN AUTOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21240">
        <property type="entry name" value="Nup98_GLEBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="7" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0811">Translocation</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="62"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="640"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="746"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="62"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="646"/>
            <end position="661"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7743034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="853" mass="86600" checksum="6A3A1E6356DAF6E6" modified="2024-05-29" version="1">MSTFGLKTGSSFFAGTSTGVFGTSLASSPFGGMSTSAAPAFASSMPAFGASSSPAFGPSSSSSDGSSVFGQMPAPSGFGSTTHSSSFESSCQQTQPAFGAPSTPAFSFSSNPAFGASSTAACGASSTPAFGSSTRAFGSSPAPVSSATSSHFSFGSSPAFGQSTSAFGSNPFGATSTSAFSSTSTPTFGNTESAFGVSSTSVFGSSTPAIGASSTPVFSASSTSACGVSSTPAFGVSSTPAFGSSPAPVSSATSSHFSFGSSPAFGQSTSAFGATSTPTFGSTSTPTFGNTGSAFGVSSSSVFGSSCPAFGSFSTPAFVASSTPDFVASSTPAFGASSSPAFGSSPASSAFGATSSSFSFWPSPTFCQSTSAFGSSPSGATSTAFGAQSYPFGGQTTTPAFGSPWFGPPSFGCQKQGGTRVTPYAQTPEADSGSWPQPARKLESISAMPAYKEKSHEELRWEDYQLGDKVGLNPAAKSSGRMSFNMNNTQQTNPFVSSSPACGQSSANMFSSTTSTNPFPPMTSTSGTLWFKSSTPSLGSPSPSFGAATSNPFWSTPSPAPSAFGSTPAFGSTSAFASSTVFPSPHLFWSAAPTFVQTSSPFGQSSSACGQAAPSFWQSSPAFVQSNAFGGNTFSSTPSLQSTSSMGFNQTTPSLSTPFQSAQPTQQHTCGFCSPVPLGGTSSIFYQNNIGQSSASAASQVPFTNPFGTLPTTPHIFGRVTDKRAPMKISRAFPQHLSQRRITSPARTYHPKNEGPKVSFYSDEEKSTSTTPIFGQPSFRVPQRGLTRVIPYAPTPVADGGNRTQAADKVESISAMAAYKEKSHEELRWEDYKLGDKGGPILLLNLYVEWALV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GKQ6</accession>
    <name>A0AAD5GKQ6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">4-coumarate--CoA ligase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_025480</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Phytoalexin biosynthesis; 3,4',5-trihydroxystilbene biosynthesis; 3,4',5-trihydroxystilbene from trans-4-coumarate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007779">
        <property type="protein sequence ID" value="KAI7743173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GKQ6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106286">
        <property type="term" value="F:(E)-caffeate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009698">
        <property type="term" value="P:phenylpropanoid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12118">
        <property type="entry name" value="ttLC_FACS_AEE21_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.30:FF:000008">
        <property type="entry name" value="2,3-dihydroxybenzoate-AMP ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000003">
        <property type="entry name" value="Long-chain-fatty-acid--CoA ligase FadD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006020">
        <property type="entry name" value="PRK08162.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43859:SF62">
        <property type="entry name" value="4-COUMARATE--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43859">
        <property type="entry name" value="ACYL-ACTIVATING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0587">Phenylpropanoid metabolism</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="5">
        <location>
            <begin position="25"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="6">
        <location>
            <begin position="459"/>
            <end position="532"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7743173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="579" mass="64321" checksum="CBFE9BB69FEBE248" modified="2024-05-29" version="1">MDRLAKCPSNYVPLTPLTFIKRAFMVYANRTSVIYAGVRFTWRETYERCCRLAYSLRSLNVVKNDVVSVLAPNVPALYEMHFAVPMAGAVLNAINTRLDAKNIATILLHSEAKVFFIDYQYVPLASEALRLLVAGSKGGSSAQYAMPLVIVIDDIDKPTGVRLGNLEYEQLIHHGNPGYPGEDLEDEWDAIALNYTSGTTSAPKGVVYSHRGAFLSTMSLIQGWEMSTEAVYMWSLPMFHCNGWTFTWGVAARGGTNVCIRNTTADEMYKGISKHKVTHMCCAPIVFNILLEAKPHERCEIKNKVNILTGGAPPPAPLLEKMETLGFHIMHAYGLTEATGPALVCEWQTKWNQLPQEQQARLKARQGVGILTLADVDVKNKDTMESVPHDGMTMGEIVLRGSSVMKGYLKDEKATAKAFQNGWFLTGDVGVIHPDGYLEIKDRSKDIIISGGENISSVELESILFKHPAILEAAVVAMPHPRWGESPCAFVVLKKTATEADILAYCRKKMSKFMVPKKVEFVKELPKTGTGKIQKVELRKIAQTLKISENTSTNNKVSEKEIHQDEVDDQHKLLAMSRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GKW4</accession>
    <name>A0AAD5GKW4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein transport protein SEC23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">M8C21_018591</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">AA19_3_7</strain>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasmic vesicle</location>
            <location evidence="9">COPII-coated vesicle membrane</location>
            <topology evidence="9">Peripheral membrane protein</topology>
            <orientation evidence="9">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Endoplasmic reticulum membrane</location>
            <topology evidence="9">Peripheral membrane protein</topology>
            <orientation evidence="9">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the SEC23/SEC24 family. SEC23 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007449">
        <property type="protein sequence ID" value="KAI7744729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GKW4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030127">
        <property type="term" value="C:COPII vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070971">
        <property type="term" value="C:endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090110">
        <property type="term" value="P:COPII-coated vesicle cargo loading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11280">
        <property type="entry name" value="gelsolin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01468">
        <property type="entry name" value="trunk_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000036">
        <property type="entry name" value="Protein transport protein SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.730">
        <property type="entry name" value="Sec23/Sec24 helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.380">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012990">
        <property type="entry name" value="Beta-sandwich_Sec23_24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036180">
        <property type="entry name" value="Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037364">
        <property type="entry name" value="Sec23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006900">
        <property type="entry name" value="Sec23/24_helical_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036175">
        <property type="entry name" value="Sec23/24_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006896">
        <property type="entry name" value="Sec23/24_trunk_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006895">
        <property type="entry name" value="Znf_Sec23_Sec24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036174">
        <property type="entry name" value="Znf_Sec23_Sec24_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11141">
        <property type="entry name" value="PROTEIN TRANSPORT PROTEIN SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11141:SF6">
        <property type="entry name" value="PROTEIN TRANSPORT PROTEIN SEC23 A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08033">
        <property type="entry name" value="Sec23_BS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04815">
        <property type="entry name" value="Sec23_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04811">
        <property type="entry name" value="Sec23_trunk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04810">
        <property type="entry name" value="zf-Sec23_Sec24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82754">
        <property type="entry name" value="C-terminal, gelsolin-like domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81811">
        <property type="entry name" value="Helical domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82919">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 9" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="3 9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 9" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 9" id="KW-0813">Transport</keyword>
    <keyword evidence="4 9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zinc finger Sec23/Sec24-type" evidence="11">
        <location>
            <begin position="188"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 trunk" evidence="12">
        <location>
            <begin position="270"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 beta-sandwich" evidence="14">
        <location>
            <begin position="513"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 helical" evidence="13">
        <location>
            <begin position="619"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="34"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF04810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF04811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF04815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAI7744729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="867" mass="94026" checksum="8A209421EB1B005B" modified="2024-05-29" version="1">MADQQVNFPPGFAVGINPSTPEPTQSRRPDTKLSPPPFTPPTAPRFAPSTVQPPVKGSQLPLSSNGVRTVSPLPHLSTPPGPPVFSSPVQPAAGPFRTSPVTPQPVAFSAAPATPPTGYSDGTNEYQHQVQSNAEDVVSLSDAPNVLLSARKVLKQKKLMNVPSLGFGALVCPGREVLQGPQIIQQDPQRCQNCGAYANLYCNILLGSGQWQCVICRNLNGSEGRYIAPTKEDLLNLPELAFPMVDFVQTGNRRPGFIPVSDSRMSAPVVLVVDDCLDEPHLQHLQSSLHAFVDSLPPTTRIGVVSYGKMVSVYDLSEGSIASADLLPGNVSPSQESLKQLIYGAGIYLSPIHASLPVVHSIFSSLRPYNSNVPEASRDRCMGAAVEVALGIIQGPSAEMSQGVVKKPGGSSRIIVCAGGPNTYGPGSVPHSFSHPNYPHMEKTALKWMEHLGREAHKRNTQIDILCAGTCPVRVPVLQPLAKASGGILILHDDFGEAFGVNLQRASTRAAGSHGLMEIRCSDDVTVSQVIGPGEEAHTDNHEAFKNDNSVSIQMLSVEETQSFAVSMETRGNIKTDFVYLQFGILFSNLYQADITRVITVRLPTVDSVSAYLDSVHDEVAAVLIAKRSLLRAKTGPDAIDVRTTIDERIKDITSRFGSQMPNSKLYQFPKELSHIPEILFHLRRGPLLGSIVGHEDERSVLRDIFLNASFDLSLRMVAPRCLMHREGGTFEELPAHDLAMQSDAAVVLDHGTDVFIWLGAELAAQEGKSAAALAACRTLAEELTEMRFPAPRILAFKEGSSQARYFVSRLIPAHKDPPYEQEARFPQLRTLSAEQRAKLKSSFIHFDEPSFCEWMRGLKVLAPEAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GMC1</accession>
    <name>A0AAD5GMC1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phytocyanin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_019599</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007241">
        <property type="protein sequence ID" value="KAI7745451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GMC1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13920">
        <property type="entry name" value="Stellacyanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000034">
        <property type="entry name" value="Cupredoxin superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039391">
        <property type="entry name" value="Phytocyanin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003245">
        <property type="entry name" value="Phytocyanin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33021">
        <property type="entry name" value="BLUE COPPER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33021:SF543">
        <property type="entry name" value="PHYTOCYANIN DOMAIN, CUPREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02298">
        <property type="entry name" value="Cu_bind_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51485">
        <property type="entry name" value="PHYTOCYANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042214332" description="Phytocyanin domain-containing protein" evidence="3">
        <location>
            <begin position="27"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="Phytocyanin" evidence="4">
        <location>
            <begin position="28"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7745451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="191" mass="19823" checksum="654A24B219F2EB8F" modified="2024-05-29" version="1">MAGLKSTMVVLVIMMIASFQVRSTMAQTRHIVGDSLGWTIPSGGAATYATWASQQTFTVGDTLFFNFTSGMHNVAEVSQTAYSPCASTNTLSLNPTGPATVTLTRPGPHYYICTIVSHCQIGQKLTINVSPLSVATPESTPLSPQSIIATPPSAFELSPTGSAIPPQSSASPPFAAAVPATLLAVALAAFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GMU6</accession>
    <name>A0AAD5GMU6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">ADP-ribosylation factor 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_014989</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006467">
        <property type="protein sequence ID" value="KAI7748767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GMU6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016004">
        <property type="term" value="F:phospholipase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003500">
        <property type="entry name" value="ADP-ribosylation factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11711">
        <property type="entry name" value="ADP RIBOSYLATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 12" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0519">Myristate</keyword>
    <keyword evidence="5 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="24"/>
            <end position="31"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="101"/>
            <end position="104"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00040200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7748767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="157" mass="17410" checksum="62F264749A51D512" modified="2024-05-29" version="1">MGLVFTRLFSSLFGNKEARILVLGLDNAGKTTILYRLQMGEVVSTIPTIGPYWRCYFPNTQAIIYVVDSSDTERLVIAKEEFHAILEEEELKGAVVLIFANKQDLPGALDDAAVTESLELHKIKNRQWSIFKTSAIKGEGLFEGLDWLSNTLKSGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GNU2</accession>
    <name>A0AAD5GNU2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_022023</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006957">
        <property type="protein sequence ID" value="KAI7746518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GNU2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="167"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="197"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7746518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="235" mass="27066" checksum="6B3CFB5442663500" modified="2024-05-29" version="1" fragment="single">MEFRIDLERKRREIILDLGLNTVDNLILILRMTRNAEGSNKRTGDDSAVEAAGLANEPSGALVLAGSRTKRPDILNRHSENRFFYAEARFYMKRVKAALHSWFFLPQTMLHVVDQITPPNSVDYKSQLLDVLKTIKYLVEKSHSKHPTPFVLISSDRRIILSETNSVCTFVLNLAVFLLVAKTCALTMNVAGVVKNWLLIAFSWPVIKDIVKPINLFGHLRQKKQRRWRHRLMMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GVC1</accession>
    <name>A0AAD5GVC1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">WAT1-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_028477</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the drug/metabolite transporter (DMT) superfamily. Plant drug/metabolite exporter (P-DME) (TC 2.A.7.4) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000185">
        <property type="protein sequence ID" value="KAI7757077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GVC1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030184">
        <property type="entry name" value="WAT1-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31218">
        <property type="entry name" value="WAT1-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="48"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="79"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="108"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="7">
        <location>
            <begin position="19"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7757077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="129" mass="14692" checksum="0CDEFFB8D09CE54A" modified="2024-05-29" version="1">MRRTDAWSWMEDVLPFVAMLMLTCLDMVVLTIVKAAMNDGMGSIPYVVYHNALGTLILLPFFVVHIIRKVDRPPLTFRILFRFFILGLLRVCLYEILGYVGVFYSSPTMASVVGNMAPGFTFLIAVFFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GVM1</accession>
    <name>A0AAD5GVM1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Developmentally-regulated G-protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_017333</name>
        <name evidence="7" type="ORF">M8C21_033141</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007305">
        <property type="protein sequence ID" value="KAI7745271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAMZMK010005069">
        <property type="protein sequence ID" value="KAI7753941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GVM1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019003">
        <property type="term" value="F:GDP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01896">
        <property type="entry name" value="DRG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17230">
        <property type="entry name" value="TGS_DRG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000003">
        <property type="entry name" value="Developmentally-regulated GTP-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000740">
        <property type="entry name" value="Putative GTP-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1070">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045001">
        <property type="entry name" value="DRG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031662">
        <property type="entry name" value="GTP-binding_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006074">
        <property type="entry name" value="GTP1-OBG_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43127">
        <property type="entry name" value="DEVELOPMENTALLY-REGULATED GTP-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16897">
        <property type="entry name" value="MMR_HSR1_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02824">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="TGS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00905">
        <property type="entry name" value="GTP1_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51880">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OBG-type G" evidence="4">
        <location>
            <begin position="63"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="TGS" evidence="5">
        <location>
            <begin position="288"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="367"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="367"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7745271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7753941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="399" mass="44449" checksum="92CEB5F11E267702" modified="2024-05-29" version="1">MGIIERIKEIEAEMARTQKNKATEYHLGQLKAKIAKLRTQLLEPPKGSSGAGEGFEVTKYGHGRVALIGFPSVGKSTLLTMLTGTQSEAAAYEFTTLTCIPGIIHYNGTKIQLLDLPGIIEGASEGKGRGRQVIAVSKSSDIVLMVLDASKSEGHRQILTKELEAVGLRLNKKPPQIYFKKKKTGGISFNSTVQLTHVDEKLCYQILHEYKIHNAELLFREDATVDDLIDVIEGNRKYMKCIYVYNKIDVVGIDDVDKLARQPNSIVISCNLKLNLDRLLAKMWEAMGLVRVYTKPQGQQPDFSDPVVLSADRGGCTVEDFCNHIHRSLVKEVKYVLVWGISARHSPQHCGLSHVLHDEDVVQIVKKKEKESEGRGRFKSHTTGPDRIADRVKKAPLKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GVW3</accession>
    <name>A0AAD5GVW3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">LOB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_019299</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LOB domain-containing protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000093">
        <property type="protein sequence ID" value="KAI7757647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GVW3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004883">
        <property type="entry name" value="LOB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31304">
        <property type="entry name" value="LOB DOMAIN-CONTAINING PROTEIN 38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31304:SF9">
        <property type="entry name" value="LOB DOMAIN-CONTAINING PROTEIN 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03195">
        <property type="entry name" value="LOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50891">
        <property type="entry name" value="LOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LOB" evidence="3">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="129"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="156"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7757647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="178" mass="19332" checksum="716C6DA532E7390D" modified="2024-05-29" version="1" fragment="single">VLRKGCEDDCIIRPCLSWITSPKSQANATLFLSKFYGRTGLLNLIGAGPPHLRPDIFKSLLYEACGRIMNPTYGSVGLMWSGNWEQCQAAVDSVLQGSTIMEFTTDSNHDVVMPLDGCDIRHLSKHADGEHNHVKISNNSKRPRGGSGFGSGSGSAFKQVTSTRSGAEPTTCKIMRKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GXU6</accession>
    <name>A0AAD5GXU6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_016681</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000976">
        <property type="protein sequence ID" value="KAI7755608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GXU6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071818">
        <property type="term" value="C:BAT3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051787">
        <property type="term" value="F:misfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031593">
        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000154">
        <property type="entry name" value="Large proline-rich protein BAG6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019956">
        <property type="entry name" value="Ubiquitin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15204">
        <property type="entry name" value="LARGE PROLINE-RICH PROTEIN BAG6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15204:SF0">
        <property type="entry name" value="LARGE PROLINE-RICH PROTEIN BAG6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00348">
        <property type="entry name" value="UBIQUITIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="2">
        <location>
            <begin position="38"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="111"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="191"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="352"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="438"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="519"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="197"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="358"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="519"/>
            <end position="539"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7755608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="582" mass="60478" checksum="71C4E2749AF05784" modified="2024-05-29" version="1">MFTFSSFKQASDTMASNHGGKGVMDSGSSEGNNSETTVEIKIKTLDSQTYTLRVDKCVPVPALKDQIATVTGVLSDQQRLICRGKVLKDDQLLSAYHVEDGHTLHLVARQPTNPSLSSFPDHTAGDPASSTRHGPSVMVGSFNISDQGDGSLPDFSRIVSAVLGSFGAANAGSGGDAVDLQQHLSERLSRIPGLNEIRNSTAQQPDDASGRSQPNSQNAAFTIPAGVSMDPLQLPVIPDSLSTLSQYLRCLGQEFNENARIQGSSTVEESYAAPRSAAGQGGLPTPAALSEVTQSTRQLLIEQAGECLMPLPFQPGTSFGTTPTSAGTSFSLGTGLRPRNIDIRIRTGSVLSSAVNQREASGGERSAAESAASEGGTSNHQAAPGNMPTVAGSDVRMLPIRISTLPPGLAPSGSSIGSISVLYPVVERSGINGQVLNGIHPHNGENRPHLAPNHTAQQQPPEVPGTTHGLLHNGRTFSTEVPSGLGQLLSNLFPSGQNNDNGFIFQGTSVETAHTAVNSQEVPAASSQETPAASSQGTPAASDEGTFVANLLQQIMPIVTQHINSGADVQDGAAQPPSSVRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-10-08" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GY69</accession>
    <name>A0AAD5GY69_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_023582</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000677">
        <property type="protein sequence ID" value="KAI7755953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009914">
        <property type="term" value="P:hormone transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000032">
        <property type="term" value="P:regulation of secondary shoot formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03232">
        <property type="entry name" value="ABCG_PDR_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000179">
        <property type="entry name" value="ABC transporter G family member 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000059">
        <property type="entry name" value="ABC transporter G family member 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029481">
        <property type="entry name" value="ABC_trans_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034003">
        <property type="entry name" value="ABCG_PDR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013581">
        <property type="entry name" value="PDR_assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48040">
        <property type="entry name" value="PLEIOTROPIC DRUG RESISTANCE PROTEIN 1-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48040:SF44">
        <property type="entry name" value="SULFATE-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19055">
        <property type="entry name" value="ABC2_membrane_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14510">
        <property type="entry name" value="ABC_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08370">
        <property type="entry name" value="PDR_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="547"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="584"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="667"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="693"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="773"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1188"/>
            <end position="1206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1226"/>
            <end position="1246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1266"/>
            <end position="1292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1304"/>
            <end position="1325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1331"/>
            <end position="1351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1363"/>
            <end position="1382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1412"/>
            <end position="1435"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="160"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="845"/>
            <end position="1094"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7755953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="1443" mass="163595" checksum="DEAF0450387DECB2" modified="2024-05-29" version="1" fragment="single">VLMASALTGDDLMRTMSRNMSRNMSRNMSRNMSRNMSRGGSLASGSTRGWGAASIREVFTTPGTDVFQKSGREDDEDELMWAAIERLPTYDRLRKGFLTNILDDGKVVREEVDVSNLGPEERHNLMKNMQKAIDDDNERLLEQLRDRIDRVGIDIPRIEIRYQNLSIQGDVFVGSRALPTLFNSTINSIEGVLGFLRLFPSKKRVVNILHEVSGIVAPSSFVSALAGKLENDLRVMGKVTYCGHEMNEFVPQRTCAYISQHDLHHGELTVRETLDFSGRCAGVGTRYEMLAELSRREKAEGIKPDPELDAFMKAIAVSGQKSSLVTEYVLRLLGLDICSDTMVGDDMRRGISGGQKKRVTTGEMLVGPAKVFLMDEISTGLDSSTTFQIIKYMRQMVHIMDITMIISLLQPAPETFELFDDIVLLSEGQVVYQGPRTNVLEFFEHVGFKCPERKGVADFLQEVTSKKDQEQYWFNKSIAYRYVSVDDFVHHFQNYHIGRKIAGILHVPYDKSKAHPAALVTNKYGISNKELFKACLSREWLLMKRNAFVYIFKTTQITIMALFTFTVFFRTTMKYGDLDDGGKYYGALFFSLINVMFNGMAELALTIFRLPVFYKQRDSLFYPAWAFALPVWLLRLPLSLLESGIWILLTYYTVGFAPSASCFFRQLLAYLGIHQMALGLFRFIAALGRTQVVANTLGTFTLLLVFVLGGFIIAKDDLQPWMKWAYYLSPMSYGQNAIVLVEFLDKRWSAPIIGSTVGKELLKARGMFTEGHWYWTCVLALFGFSLIFNLCFMLALTYLTPMVDSKSIVDDNDEKNKKLAKIESESEPNGLNKKGMVLPFKPLSLVFDHVNYYVDMPASQGVEEDRLQLLRDVSGAFRPGVLTALVGVSGAGKTTLMDVLAGRKTGGYIEGSISISGYPKNQETFARVSGYCEQNDIHSPHVTVYESLLYSAWLRLASDVDEATRKMFVEEVMELVELGPIRNALVGLPGVDGLSTEQRKRVTIAVELVANPSIIFMDEPTSGLDARAAAIVMRAVRNTVDTGRTVVCTIHQPSIDIFEAFDELFLMKRGGQAIYAGPLGRYSHLLVEYFESIDGVSKIKEGYNPATWMLEVTSTAVESQLGVDFAEIYANSDLYRRNEELIKELSTPPPDSQDLHFATQYSQPSFEQFKACFWKQHKSYWRNPQYNVVRFFMTTVIGLIFGIIFWNKGTKISKQQDLSNLMGAMYAAVLFLGGTNTSAVQSVVAIERTVFYREKAAGMYSALPYAFAQALVETIYVGIQTFLYSLLLYYMIGFEWQLGKFLWFYYYVFMCFVYFTLYGMMLVALTPSYQIAAIVMSFFLSFWNLFSGFLIPRGQIPIWWRWYYWGSPVAWTIYGLITSQLGDMSTRVFVPKHGLMEVKVYLKEFLGYEYDFLGYVALAHVGWVLLFFGVFVYGIKALNFQRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5H0R4</accession>
    <name>A0AAD5H0R4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SP-RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_013086</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000003">
        <property type="protein sequence ID" value="KAI7758398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5H0R4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061665">
        <property type="term" value="F:SUMO ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16650">
        <property type="entry name" value="SP-RING_PIAS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004181">
        <property type="entry name" value="Znf_MIZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10782">
        <property type="entry name" value="ZINC FINGER MIZ DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10782:SF82">
        <property type="entry name" value="ZINC FINGER, MIZ-TYPE, ZINC FINGER, RING_FYVE_PHD-TYPE, E3 SUMO PROTEIN LIGASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02891">
        <property type="entry name" value="zf-MIZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51044">
        <property type="entry name" value="ZF_SP_RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SP-RING-type" evidence="6">
        <location>
            <begin position="339"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="463"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="617"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="463"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="643"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="656"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7758398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="656" mass="71377" checksum="A4DA4B60C91B4A73" modified="2024-05-29" version="1" fragment="single">MYYACTFQHPLVGSSFNTPSPLCYLLSTAFFHTLCKQATEEEDLSMATAAAVAVAVACLRRRVASTKETALKRMESSIDQASLSIHNGDIHNPTVYSQTCIKLGSSIDLAIAMNASPSSFHMPQLVLIVKEVYRLKTELLQPSLMLLLLPIKAACKIGWFPDGDKDCLLMIAKEVSYGFCSTDKMNIEPSNAHSSVSNIVSRFYPMMKVEHILTSFDVKAGYGTFAVDFHISLGATPRHCRNLWLLVAQLDNMETAACITNPLKVDFLINGCEVPNRTRNNMGNGPQLPSNITKMVKHGVNLIQAFGNFDGRYIIALVFMNSISSRDPSQLQDYLRPMSDCAIIEMSSRISLYCPISRHRIKTPVKGHLCKHSQCFDYNCFIEVNSTKPTWECPVCNNPILNDVAEDVHDVIISEDGSWMVAETNGDTMSHTTETTPAVSLGHTRVNLGTNKQAQNTTVFTPTAGSLGQAQGNTCTTNQGQSVTMFTPPAAETKPEVPPSQTQVDGCTTMQVKSTMMLTPPEGSNQIRVNVCTSKQGQNTTFTPPATVKAEVSPGEAQADICTTTQAKGTTMLTPPEGSPAQIQFDVCKSNQGQRTAFTQPATRETETEVNVCSPMFTPPAFTTPRSFAPASNELRKGGKIGSHTTPGESQPAPNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BKA6</accession>
    <name>A0AAD5BKA6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Core-2/I-branching beta-1,6-N-acetylglucosaminyltransferase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_031225</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012160">
        <property type="protein sequence ID" value="KAI7724800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BKA6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044174">
        <property type="entry name" value="BC10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003406">
        <property type="entry name" value="Glyco_trans_14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31042">
        <property type="entry name" value="CORE-2/I-BRANCHING BETA-1,6-N-ACETYLGLUCOSAMINYLTRANSFERASE FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31042:SF8">
        <property type="entry name" value="CORE-2_I-BRANCHING BETA-1,6-N-ACETYLGLUCOSAMINYLTRANSFERASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02485">
        <property type="entry name" value="Branch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7724800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="311" mass="36006" checksum="91B52BCF54DAAFF7" modified="2024-05-29" version="1" fragment="single">GRRGIYMIRKTGVVTTVTSAIPILQPCIQQQPNNALDYWITPPSNLMHTMSDKELFWRASFVPQVKKYPFARVPKIAFMFLTKGPLPLAPIWERQIPSQIAEWGRMSMCDAERRLLANALLDISNEYFILVSESCIPIYNFTTIYRYINRSKYSFMGAFDDPGPFGRGRYNPNMLPEVNISQWRKGSQWFEVNRKLASIIISDVTYYPKFLNFCRPACYVDEHYFPTLLTIQAPNLLANRSLTWVDWSRGGPHPATFGATDITERFMMKLHEMKECVYNDKPSSVCYLFARKFAPSTLQRLLLLAPDFLGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BLZ3</accession>
    <name>A0AAD5BLZ3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_014583</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011864">
        <property type="protein sequence ID" value="KAI7725761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BLZ3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7725761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="67" mass="7122" checksum="CA3AC3FCECF223F9" modified="2024-05-29" version="1" fragment="single">GGSDPLHLLCRLVGFGGSEVRGGHRLRVTLELITDFVCVRVLSVGYGSFCSISLISISSFGSNCRYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BMK9</accession>
    <name>A0AAD5BMK9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_010947</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011838">
        <property type="protein sequence ID" value="KAI7725879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BMK9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080043">
        <property type="term" value="F:quercetin 3-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080044">
        <property type="term" value="F:quercetin 7-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926:SF1361">
        <property type="entry name" value="CROCETIN GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926">
        <property type="entry name" value="GLUCOSYL/GLUCURONOSYL TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7725879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="278" mass="31219" checksum="D29A4A92EDC6923F" modified="2024-05-29" version="1" fragment="single">FISNFATNGAHSVAQTITFTVAADQPFHHLVYTSGIPWAAKVAHSYKHKSSLLWCQPATVLNIYYYYFNGYEDLISNNKNNSSFLIKLPGLPPLTMDDLPSFLSPSRLKEHDFMMQVLKDHVDALKTAPRIPELEVESIKAIEQVEFLPIGPLIRSDGKEKDGCIRWLDTNPEASVVYVAFGSQAILSMEQVEEIAIGLLASGRPFLWVIRDRVRVQRRQGDGVVERKEIDSCVKMVMAGDDGGDIRRNAYKWRDLATEALNTNRSSTVNLQAFLDDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BMM2</accession>
    <name>A0AAD5BMM2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_016639</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012013">
        <property type="protein sequence ID" value="KAI7725196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BMM2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7725196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="109" mass="12594" checksum="C9B12A552263938C" modified="2024-05-29" version="1" fragment="single">IFFVGLLKVVLPIAEDVIDFKSRFHNNKEELGRVRYWRKLPSFVDAYLCFEVDAIRCAFGILWTGLITRPKKHTKIEVDDHSQLAVSTALWCNFLVFSLKFGVWIATSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BPR6</accession>
    <name>A0AAD5BPR6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ALBINO3-like protein 2, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_006599</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the OXA1/ALB3/YidC (TC 2.A.9.2) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011772">
        <property type="protein sequence ID" value="KAI7726034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BPR6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032977">
        <property type="term" value="F:membrane insertase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032979">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane from matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20069">
        <property type="entry name" value="5TM_Oxa1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001708">
        <property type="entry name" value="YidC/ALB3/OXA1/COX18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12428:SF65">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX18, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12428">
        <property type="entry name" value="OXA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="155"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="218"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="273"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="313"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7726034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="535" mass="59573" checksum="C89B154DBDFBA05F" modified="2024-05-29" version="1">MAMRKQLSRFNLVHLRHHTSRILASSSIPSSSIEPYSPSSSIEPQHFTNTSNRYLSTLGFYRHIQFIKPHNFLLLSNYRSFSTNESKATDAEQLSDPGFFDYDYGGGGGGGEVIGAIGSGDEVIGALGSGDEAILPIRCLIWFLDTVHDLTGMPWWMVIVISTLALRVVILPVLLVQLRLLKKNAELAPKLPPPFPPPFSGKSWIEQYKRFRDKRKEIGCPSFLWILAYPSVQIPCFILGTASVREMALDHHPGFECGGTLWFQNLIELPHGATGYIFPLLLASLHLANVQVSFDKSSLTTLPNLIGVLAKYYSYYLQLLTLPILFAGFYLPQGSLIYWTANSSITLFQQLAMKNPHVREKLGLPDKLAAVDSGKSHELDGLESTFTVPLVKKPIQELSALELYNLSIKHLSKGERDRAVQLIRLILQKDPEYVSALIVMGQILIKEEKLAAATEYLERAIAKLLLVGHPTEVEEIDHLILASLWSGVALAKQGKVAEGITHLERIANMKEPEDAKTKTHYCDGLLVLSSNRYEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BQ76</accession>
    <name>A0AAD5BQ76_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_016221</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protease inhibitor I13 (potato type I serine protease inhibitor) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011642">
        <property type="protein sequence ID" value="KAI7726531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BQ76"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009611">
        <property type="term" value="P:response to wounding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.10.10">
        <property type="entry name" value="Trypsin Inhibitor V, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000864">
        <property type="entry name" value="Prot_inh_pot1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036354">
        <property type="entry name" value="Prot_inh_pot1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33091">
        <property type="entry name" value="PROTEIN, PUTATIVE, EXPRESSED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33091:SF83">
        <property type="entry name" value="SERINE PROTEASE INHIBITOR, POTATO INHIBITOR I-TYPE FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00280">
        <property type="entry name" value="potato_inhibit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00292">
        <property type="entry name" value="POTATOINHBTR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54654">
        <property type="entry name" value="CI-2 family of serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00285">
        <property type="entry name" value="POTATO_INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0722">Serine protease inhibitor</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7726531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="65" mass="6978" checksum="1E3F4908AD191BE8" modified="2024-05-29" version="1">MGKSSWPELVGEKGEVAAAIIEKENPLVNAIVILDGTAVTMDFRTDRVRVVVNTEGVVIQTPVIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BQN5</accession>
    <name>A0AAD5BQN5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Heat shock protein 83</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_021807</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the heat shock protein 90 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011335">
        <property type="protein sequence ID" value="KAI7727695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BQN5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16927">
        <property type="entry name" value="HATPase_Hsp90-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000005">
        <property type="entry name" value="Heat shock protein 90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.790:FF:000001">
        <property type="entry name" value="Heat shock protein 90 alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.80:FF:000001">
        <property type="entry name" value="Heat shock protein 90 alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11260:FF:000001">
        <property type="entry name" value="Heat shock protein 90 alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.790">
        <property type="entry name" value="Heat shock protein 90, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00505">
        <property type="entry name" value="HSP90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037196">
        <property type="entry name" value="HSP90_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001404">
        <property type="entry name" value="Hsp90_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020575">
        <property type="entry name" value="Hsp90_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003555">
        <property type="entry name" value="PRK05218.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11528">
        <property type="entry name" value="HEAT SHOCK PROTEIN 90 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13589">
        <property type="entry name" value="HATPase_c_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00183">
        <property type="entry name" value="HSP90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002583">
        <property type="entry name" value="Hsp90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00775">
        <property type="entry name" value="HEATSHOCK90"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110942">
        <property type="entry name" value="HSP90 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="144"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="600"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="600"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="29"/>
            <end position="30"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="49"/>
            <end position="54"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="300"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR002583-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7727695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="626" mass="72019" checksum="2EF6E355036641A9" modified="2024-05-29" version="1" fragment="single">ANKTLSIIDSGVGMTKADLVNNLGTIARSGTKEFMEALQAGADVSMIGQFGVGFYSAYLVAEKVIVTTKHNDDEQYIWESQAGGSFTVTRDVDGEALGRGTKITLFLKEDQMEYLEERRIKDLVKKHSEFISYPIYLWTEKTTEKEISDDEDEEPKKEEEGDIEEVDEEKEKEKGKKKKIKEVSHEWELINKQKPIWLRKPEEITKEEYAAFYKSLTNDWEEHLAVKHFSVEGQLEFKAILFVPKRAPFDLFDTRKKMNNIKLYVRRVFIMDNCEELIPEYLGFVKGVVDSDDLPLNISREMLQQNKILKVIRKNLVKKCIEMFSEIAENKEDYNKFYEAFSKNLKLGIHEDSQNRSKLADLLRYHSTKSGDELTSLKDYVTRMKEGQKDIYYITGESKKAVENSPFLERLKKKGYEVLFMVDAIDEYAVGQLKEYDGKKLVSATKEGLKLEDETEEEKQKREEKKKSFENLCKTIKDILGDKVEKVVVSDRIVDSPCCLVTGEYGWTANMERIMKAQALRDSSMGAYMSSKKTMEINPDNPIMDELRKRAEADKNDKSVKDLVLLLFETALLTSGFSLEDPNTFGGRIHRMLKLGLSIDEEEGEDAEMPALEEEGAEESKMEEVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BR40</accession>
    <name>A0AAD5BR40_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 15">Pyruvate kinase</fullName>
            <ecNumber evidence="5 15">2.7.1.40</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">M8C21_002802</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">AA19_3_7</strain>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>pyruvate + ATP = phosphoenolpyruvate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18157"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.40"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 15">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 15">Belongs to the pyruvate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.40" evidence="5 15"/>
    <dbReference type="EMBL" id="JAMZMK010011282">
        <property type="protein sequence ID" value="KAI7727957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009570">
        <property type="term" value="C:chloroplast stroma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030955">
        <property type="term" value="F:potassium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004743">
        <property type="term" value="F:pyruvate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.60:FF:000025">
        <property type="entry name" value="Pyruvate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="entry name" value="Phosphoenolpyruvate-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.33.10">
        <property type="entry name" value="PK beta-barrel domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1380.20">
        <property type="entry name" value="Pyruvate kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001697">
        <property type="entry name" value="Pyr_Knase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011037">
        <property type="entry name" value="Pyrv_Knase-like_insert_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018209">
        <property type="entry name" value="Pyrv_Knase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015793">
        <property type="entry name" value="Pyrv_Knase_brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015795">
        <property type="entry name" value="Pyrv_Knase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036918">
        <property type="entry name" value="Pyrv_Knase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015806">
        <property type="entry name" value="Pyrv_Knase_insert_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11817">
        <property type="entry name" value="PYRUVATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00224">
        <property type="entry name" value="PK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02887">
        <property type="entry name" value="PK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01050">
        <property type="entry name" value="PYRUVTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50800">
        <property type="entry name" value="PK beta-barrel domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52935">
        <property type="entry name" value="PK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00110">
        <property type="entry name" value="PYRUVATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12 15" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="9 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 15" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyruvate kinase barrel" evidence="16">
        <location>
            <begin position="35"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate kinase C-terminal" evidence="17">
        <location>
            <begin position="295"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF02887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAI7727957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="413" mass="45643" checksum="14153A486D460590" modified="2024-05-29" version="1">MSSMAAEFALLSSGLDLKKREGSIWLFTTEKFDGGRPFTVQANYEGFNEGVKEGDELVVDGGMATFEVVERVGNDLRCKCTDPGLLLPRAKFSFWRDGKLVEKHHELPTFSEKDWSDIEFGISEGVDFIALSFVKDAAAVKHLKDYLLTKNKSVKVLAKIESLESLKNLEEIVEASDGIMXXXXIPLEQVPAVQEEIIDLCRKLNKPVIIASQLLESMIEYPTPTRAEVADVSEAVRQRADALMLSGESAMGSYGQKAISVLRMTSARMELWGREENQQSFLPQIGESLPDQVAEQICSCACQMANKLGVDAIFVYTTHGQMASLLCRNRPNPPIFAFTNKKSTQMALTLEWGVVPIAFDLSEDMDANVSTTTHLMKAKGVMRQGDVILVVSDVIPMSVTPTVYQSLQVVVIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BRF4</accession>
    <name>A0AAD5BRF4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">DEAD-box ATP-dependent RNA helicase 7</fullName>
            <ecNumber evidence="3">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">M8C21_028624</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">AA19_3_7</strain>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DEAD box helicase family. DDX21/DDX50 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010011269">
        <property type="protein sequence ID" value="KAI7728122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BRF4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00268">
        <property type="entry name" value="DEADc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12937">
        <property type="entry name" value="GUCT_RH7_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2280:FF:000003">
        <property type="entry name" value="DEAD-box ATP-dependent RNA helicase 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059027">
        <property type="entry name" value="DD_DDX21-DDX50"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044742">
        <property type="entry name" value="DEAD/DEAH_RhlB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050547">
        <property type="entry name" value="DEAD_box_RNA_helicases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012562">
        <property type="entry name" value="GUCT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47963:SF8">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47963">
        <property type="entry name" value="DEAD-BOX ATP-DEPENDENT RNA HELICASE 47, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26142">
        <property type="entry name" value="DD_DDX21-DDX50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08152">
        <property type="entry name" value="GUCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="13">
        <location>
            <begin position="148"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="14">
        <location>
            <begin position="301"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="588"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="41"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="596"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="620"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="631"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="15">
        <location>
            <position position="643"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00070889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAI7728122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="643" mass="69358" checksum="20A89B50057C7195" modified="2024-05-29" version="1" fragment="single">MPSLANLSETQSIETKVKKMKSETAALESANKKKEKKSKKNKTEISSDSEKSDKKIKKKRKASSDSDNDEGKSDTSSELCEPVNLKIEESVKKSKKKKMKTGEVVEKAVEIKEENPNAVSNFKISEPLKNALKAKGIEALFPIQARTFESIYDGLDLIGKAKTGQGKTLAFILPILESLTSGIEKASRRTGYGRSPTILVLLPTRELAKQVYTDFKYYGEAVGLSSCCLYGGGGASISPQTVQLKRGVDIVVGAVGRVKIASKFLKPNKKIVDLVGVQVMKASESVRHIIMPCSWTARSQVIPDIIRHHSSGGRTIVFTETKDYCSELSGVLTGARPLHGDIQQSVREATLAGFRTGKFMTLVATNVAARGLDINDVQLIIQCEPPRDVEDYIHRSGRTGRAGKSGVAITLYEPRKSNISKLEREAGVKFEHLSAPQPADIAKAAGEDAAEAIIQVADSVIPVFKSAAEELLNSSGLTPVELLAKALAKSMGYTEIKKRSLLTSMENYVTLLLEAGRPVYTPSFAYGVLRRFLPEEKVESIQGLALTADQRGAVFDVAAEDLDTFLAGQENAAGVSLEVVKELPQLQEKEQSRGRFGSGGGFSRGRGGGGGFSRGGRGGFSDRRNDRFSRGSGGSGGRGGYKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BT08</accession>
    <name>A0AAD5BT08_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_015525</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
            <orientation evidence="1">Nucleoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NEMP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011144">
        <property type="protein sequence ID" value="KAI7728789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BT08"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005637">
        <property type="term" value="C:nuclear inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019358">
        <property type="entry name" value="NEMP_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31587">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN (DUF2215)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31587:SF4">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN (DUF2215)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10225">
        <property type="entry name" value="NEMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="165"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="192"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="224"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="264"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="296"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="323"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7728789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="451" mass="49047" checksum="4E37C1904B98E1B3" modified="2024-05-29" version="1" fragment="single">MGGGETVAYVSFPAILLFLLTVVNSAGVGVSGELPSLTVAKFASLQLSPSLAVKNSPGSRPGSQVACERLEIIGLPRRRNLTSFFSSVKVKVTNVNSTGRPPSITVCFHRNASLGVGMCPEDRWEKLTNGSWVRSMSPFDHKLLDIRSVGPSVGTVEVALNEEFYLYRVIFLILGITMMTFATSLSNSLATYYGGAVTLGVLLVILVILFQGMKILPSGRKSSLALVLYGSIVGVGSFLFSYLPALLRSLLVEMGISEDLHSPVAAFLLAFVVLCGAWLGFWAVRKLVLTEDGSIDTGVAQFVAWSIRIVASCMILQCSVDPLLAVAALVCGVVIPSAAKGLLNYYAPKSLRVKHIRSHKPVSSSPIENASHYKNKDDLKLSDSNTFYSTFHNTPSRKQYSKEEWDKFTKDSTKKALESLVWSPDFNRWAVANADRLALLPKKDTSPRRKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BTQ7</accession>
    <name>A0AAD5BTQ7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_033183</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011062">
        <property type="protein sequence ID" value="KAI7729134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BTQ7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009943">
        <property type="entry name" value="DUF1475"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36318">
        <property type="entry name" value="OS06G0581300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36318:SF5">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="102"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7729134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="137" mass="15184" checksum="F8029547E57BDD94" modified="2024-05-29" version="1">MAASTTSVSMSVALKTFFIVSAWVMAAVLVYSYAADGEASCYDPRAWWMQVAETDFMVLILPIIALFFTKESSWLKRIVFMIVSLFGGRDVNMGRARRSSLVIAKVIVTAIGCYSLGLIVYAYITDGFPFHMKVLTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BWR3</accession>
    <name>A0AAD5BWR3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="5">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">M8C21_013481</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="25">AA19_3_7</strain>
            <tissue evidence="25">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the C-terminal section; belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the leguminous lectin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="25">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="5"/>
    <dbReference type="EMBL" id="JAMZMK010010608">
        <property type="protein sequence ID" value="KAI7730867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BWR3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002229">
        <property type="term" value="P:defense response to oomycetes"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06899">
        <property type="entry name" value="lectin_legume_LecRK_Arcelin_ConA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000108">
        <property type="entry name" value="L-type lectin-domain containing receptor kinase S.4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000086">
        <property type="entry name" value="L-type lectin-domain containing receptor kinase S.4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000178">
        <property type="entry name" value="serine/threonine-protein kinase PBS1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050528">
        <property type="entry name" value="L-type_Lectin-RKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001220">
        <property type="entry name" value="Legume_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27007">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00139">
        <property type="entry name" value="Lectin_legB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0430">Lectin</keyword>
    <keyword evidence="16 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="12 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-0675">Receptor</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10 23" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 22" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="23">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042157308" description="non-specific serine/threonine protein kinase" evidence="23">
        <location>
            <begin position="29"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="293"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="24">
        <location>
            <begin position="350"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="379"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="KAI7730867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="639" mass="71492" checksum="5AD16C7B5E01B397" modified="2024-05-29" version="1">MLLPPIPWLHLLLSLSLSLSLAIQLALSQSFIYNTFKPFHPSNLTTNGITTITNNGILQLTNVSSRQIGRGFYPDPIRFKNPNTQTALSFSTAFVFSIVPKHPKLGGHGLAFTISPTKDFIGAQHSQYLGLVNVTHNGNTSNHLFAVEFDTVQDLDFFDINDNHVGVNINGMKSVNSTKSGFFIDGNVTKQDLCLHCGKKIQAWVDYDGDHHQLNITLSLFSNKPTTPIMSVKVDLSSVFQDFMYVGFSSSTGLLASSHYVFGWSFNMTGYAQSLDLDKFPSLKPLEKNKQGFVIRFAASMFLAISVMIISGVFYVKKKFRNIEVVEDWELDLGPNPYNYKVLKEATKGFNDQQLLGFGGSGKVYKGVLADSKTEIAVKRISEQSKQGQKEFLSEVSTIGRLRHRNLVQLLGWSRNKGELLLIYDYMANGSLDKYIHSGNTPNMILSWEQRFNIIKDVSNGLLYLHEEWEQTVLHRDIKAGNVLLDSQLKGKLGDFGLAKLYEHGSNPTTTKVVGTLGYLAPELTRTGKPTTRSDMYAYGALLLEVVCGRKPIELKASPEELILVDWVWDKWREGRLLEVVDSRLKGEFNEVEVMVVLKVGLMCSSDEHSCRPSIRQVIRYLEGEVSLPKSFGQSCAEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BYN1</accession>
    <name>A0AAD5BYN1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NADH-ubiquinone oxidoreductase chain 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">NADH dehydrogenase subunit 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_010695</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a ubiquinone + NADH + 5 H(+)(in) = a ubiquinol + NAD(+) + 4 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29091"/>
            <dbReference type="Rhea" id="RHEA-COMP:9565"/>
            <dbReference type="Rhea" id="RHEA-COMP:9566"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <dbReference type="ChEBI" id="CHEBI:17976"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="7.1.1.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complex I subunit 3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010620">
        <property type="protein sequence ID" value="KAI7730821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BYN1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030964">
        <property type="term" value="C:NADH dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1610">
        <property type="entry name" value="NADH:ubiquinone/plastoquinone oxidoreductase, chain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000440">
        <property type="entry name" value="NADH_UbQ/plastoQ_OxRdtase_su3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038430">
        <property type="entry name" value="NDAH_ubi_oxred_su3_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11058">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11058:SF9">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00507">
        <property type="entry name" value="Oxidored_q4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-1278">Translocase</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="48"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="72"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7730821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="106" mass="12275" checksum="E9A49EEA8B351DC8" modified="2024-05-29" version="1" fragment="single">SASIFLLYEYDIFFGISNNIKFSSYFVPSSKGPEKLSGDAWLQFRIRYYMFALVFVVFDVETVFLYPWAMSFDVLCVIVFVEDLTFVLISIVALVYAWRKGALECS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-10-08" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BYQ9</accession>
    <name>A0AAD5BYQ9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CBS domain-containing protein CBSX5-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_022437</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010401">
        <property type="protein sequence ID" value="KAI7731684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050511">
        <property type="entry name" value="AMPK_gamma/SDS23_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13780">
        <property type="entry name" value="AMP-ACTIVATED PROTEIN KINASE, GAMMA REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13780:SF132">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0129">CBS domain</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7731684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="405" mass="43725" checksum="0BC3C56792F7D316" modified="2024-05-29" version="1">MAARLLAHEVADLCLGKPPLKSLPVTATVRHALTALKATDDTHISVWNCCNHHHPISDQSDCRCVGKICMVDIICYLCKDDNITSPSSVLDSPVSVLLSPDSNVVRHVDPSTCLVEAIDMIINGAQNLVVPISSRTTIKSKRKQQQPIHDGLTIAPTTHAGGVEFCWLTQEDVIRFLLNSIALFSIATYSVNSLGIIDSDILTVNYHSPAATALESIITSLNDQTSVAVVDEFGILIGEISPFTLSYCDETAAAAITTXXXXSGDLMAYIDCGGPPEDIIKLVEMRLKQRNLKGMLEGFSVYSSGIPLNNANSCSSSSDDELVNSPVSVKSGRLSRSSSYSARITRMAEAIVCYPGSSLVAVMIQAIAHRVSYVWVIEEDCSVVGIVRFASMLEVFRSHLESMMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BZ69</accession>
    <name>A0AAD5BZ69_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">M8C21_002579</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">AA19_3_7</strain>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Secondary metabolite biosynthesis; terpenoid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010483">
        <property type="protein sequence ID" value="KAI7731303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BZ69"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016712">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051762">
        <property type="term" value="P:sesquiterpene biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11072">
        <property type="entry name" value="CYP71-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000011">
        <property type="entry name" value="Cytochrome P450 83B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47955:SF22">
        <property type="entry name" value="CYTOCHROME P450 83B1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47955">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 71 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13 14" id="KW-0349">Heme</keyword>
    <keyword evidence="10 13 14" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 13 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 14" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="9 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042223807" description="Cytochrome P450" evidence="15">
        <location>
            <begin position="28"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="13">
        <location>
            <position position="443"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAI7731303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="501" mass="57589" checksum="4204F3F47BD11A07" modified="2024-05-29" version="1">MMWNIMSFLFLFLILSLFYILPKKSKFNPPGPVGLPFIGNLHQLDQSSLHTSLWNLTKPYGPIVSLRFGFNSAVVISSASLAKEVLKTQDLSFCDRPLFIGPRKLTYDGLDMAFSPYNENWREMRKIFTLHLFSPKRVQSFRYIREDEVSITMKTIHDLALSSKTVNLSEMMKKLTSIIMMRVCFGNKYNCRDGHEINKVLGLLNEVQAYLVDLYVSDIWPGLPFVGLVDRLMGKMDRQDKCFRDLDSFYQQLIDERLNTQKQESYEEDEDDLIDILLQLVKDKHFSLTHDHIKAMLMDVLIAGTDTGAAVVVWAMTSLIRNPKVMHLAQEEVRNVIGNNGKITEDDLPKLAYLKSVIKETLRLYPPAPLLVPRETRKDAIVHGYQIKQKTLVYVNAWAIARDPKSWENQEEFLPERFMGSSEIDFKGNDFELIPFGAGRRICPGMSIGVVMVELLLANLLYLFDWGLPDGVRKEDIDFDATPGITMHKRNELCLLAYVHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C0G0</accession>
    <name>A0AAD5C0G0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Receptor-like serine/threonine-protein kinase</fullName>
            <ecNumber evidence="18">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">M8C21_033486</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="24">AA19_3_7</strain>
            <tissue evidence="24">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="18"/>
    <dbReference type="EMBL" id="JAMZMK010010092">
        <property type="protein sequence ID" value="KAI7733081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01098">
        <property type="entry name" value="PAN_AP_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000237">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.90.10.10:FF:000013">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase LECRK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000059">
        <property type="entry name" value="S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.90.10.10">
        <property type="entry name" value="Bulb-type lectin domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001480">
        <property type="entry name" value="Bulb-type_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036426">
        <property type="entry name" value="Bulb-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051343">
        <property type="entry name" value="G-type_lectin_kinases/EP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024171">
        <property type="entry name" value="SRK-like_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47976">
        <property type="entry name" value="G-TYPE LECTIN S-RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE SD2-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47976:SF15">
        <property type="entry name" value="G-TYPE LECTIN S-RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE RLK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01453">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000641">
        <property type="entry name" value="SRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00108">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51110">
        <property type="entry name" value="alpha-D-mannose-specific plant lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50927">
        <property type="entry name" value="BULB_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 18 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 18" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0430">Lectin</keyword>
    <keyword evidence="12 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 18 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0675">Receptor</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 18" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6 21" id="KW-0732">Signal</keyword>
    <keyword evidence="4 18" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042124340" description="Receptor-like serine/threonine-protein kinase" evidence="21">
        <location>
            <begin position="21"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="441"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="domain" description="Bulb-type lectin" evidence="23">
        <location>
            <begin position="18"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="22">
        <location>
            <begin position="499"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="527"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRNR" id="PIRNR000641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="KAI7733081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="784" mass="87806" checksum="9913824BE11BABA8" modified="2024-05-29" version="1">MLSVSCLILLTIFIPCLVSAQQTDGSVRVGASLTAAPNARPWLSSSGEFAFGFQQVQGDDRFLLSIWYDKIPEKTIIWYPTSPTVSAGSKVELTNGSGLLLSDPRGRNVWTSGPVSVVAYAVMNDTGNFMIFGNGSGKIWESFSFPADTMLPTQVMERNGVIYSKMSKRNFSRGRFQLRLLQDGNLVLNTRDVASGYAYTDYYATKTNDPVNESNAGERLFXXXXLRRNGKRFDLTPKQELPFGDYYHRVTLDFDGVFTQYYHPKDPTDSSGWGIVWSCPENICLSFHGSEDSGACGFNNVCSLSHGNRPKCECPKGFSLVDPSNPYGDCKPYFFPYCQEGGPKRDALDFIELYDIDWPYADYVIMNPTSETDCKSSCLNDCLCGVAIYRGNKCWKKKFPLSNGKVDYSLGVKAFVKFPKGDINPPGSPQSSRANNDRQSLIIVGSTLLGTSVFVNLLLIVAICAGMFLFYHNKTRRLDPGVETNVVRFTYKELVKATNGFKDELGKGAFGIVYKGVIRTNTVAVKKLYTMVQDGEKEFRTEVNAIARTHHKNLVQLLGFCDDGDERLLVYEYMSNGTLASFLFGDMRPGWNPRCNIALGTAKGLSYLHEECSTQIIHCDIKPQNILLDEYYNARISDFGLAKLLTMNQSQTNTGIRGTKGYVAPEWFRNTPVTVMVDVYSFGVLLLEIISCRKSVKENESGDEYAAILTDWAWDCYQEKRLDLFVENDSEALDNIKKLRGFLMVGLWCVQENPSLRPTMRKVIQMLEGVVEVNEPPCPFPFVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C234</accession>
    <name>A0AAD5C234_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_025690</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">High-conductance voltage-dependent solute channel with a slight selectivity for cations transporting triosephosphates, dicarboxylic acids, ATP, inorganic phosphate (Pi), sugars, and positively or negatively charged amino acids.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homooligomers form large rather nonselective pores in plastidial outer membranes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Plastid</location>
            <location evidence="2">Chloroplast outer membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Plastid</location>
            <location evidence="3">Etioplast membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010107">
        <property type="protein sequence ID" value="KAI7732938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C234"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009707">
        <property type="term" value="C:chloroplast outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034426">
        <property type="term" value="C:etioplast membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046930">
        <property type="term" value="C:pore complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015288">
        <property type="term" value="F:porin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022843">
        <property type="term" value="F:voltage-gated monoatomic cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034765">
        <property type="term" value="P:regulation of monoatomic ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034626">
        <property type="entry name" value="OEP24"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35284">
        <property type="entry name" value="OUTER ENVELOPE PORE PROTEIN 24A, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35284:SF1">
        <property type="entry name" value="OUTER ENVELOPE PORE PROTEIN 24A, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0934">Plastid</keyword>
    <keyword evidence="10" id="KW-1002">Plastid outer membrane</keyword>
    <keyword evidence="12" id="KW-0626">Porin</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1134">Transmembrane beta strand</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7732938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="214" mass="23418" checksum="4DDC5E9953B6195E" modified="2024-05-29" version="1">MKASLKAKYDADKATAASAVTLAFNAGDVKLRASMTDATVVNGPSLNGLALAVDKPGSFSIDYNVPKKDFRFMFMNTIKVSDKPLNLMYSHSLGDQKTMLDGTLVIDSDNKVSVHHVLGSGNCKLKYTYVHGGLTTFEPSYDLAKDSWDFAVSRKVYADHVLRAAYQTSSQNLAVDWLTKSKLIGSYKVSAHFNLEGGIKVPKVTAESTWDFEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C268</accession>
    <name>A0AAD5C268_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_024989</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the iron/ascorbate-dependent oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010099">
        <property type="protein sequence ID" value="KAI7732998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C268"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031418">
        <property type="term" value="F:L-ascorbic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009813">
        <property type="term" value="P:flavonoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046148">
        <property type="term" value="P:pigment biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.330:FF:000009">
        <property type="entry name" value="Flavonol synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050295">
        <property type="entry name" value="Plant_2OG-oxidoreductases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47991">
        <property type="entry name" value="OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="8" id="KW-0284">Flavonoid biosynthesis</keyword>
    <keyword evidence="7 9" id="KW-0408">Iron</keyword>
    <keyword evidence="3 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0847">Vitamin C</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="10">
        <location>
            <begin position="210"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7732998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="356" mass="40583" checksum="8A0003D163B206B6" modified="2024-05-29" version="1">MVLSANTRVESLSKSGIQHIPKEYIRTQDELTTITDIFREENNQQCPQVPTIDLNNISSNDPKAREMCREEIVKASTEWGVMHLVNHGISLDLINRVKAAGESFFDQPVEEKEKYSNDIVSGKIQGYGSKLANNACGQLEWEDYFFHLVFPEEKRDLTIWPSTPKDYIPATTEYARQVRSLATKILAMLSLGLGLEEGRLEKEVGGMEELLLQLKINYYPKCPQPELALGVEAHTDVSALTFILHNMVPGLQLFYDGQWVTAKCVPDSIIMHIGDTIEILSNGKYKSILHRGLVNKEKVRISWAVFCEPPKEKIILKPLPETVSEEEPPLFPPRTFHQHMEHKLFRKNNDTLVQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C3A8</accession>
    <name>A0AAD5C3A8_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_017032</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009756">
        <property type="protein sequence ID" value="KAI7734327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C3A8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009723">
        <property type="term" value="P:response to ethylene"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009739">
        <property type="term" value="P:response to gibberellin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000009">
        <property type="entry name" value="transcription factor MYB1R1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006447">
        <property type="entry name" value="Myb_dom_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052245">
        <property type="entry name" value="Plant_Stress_Dev_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01557">
        <property type="entry name" value="myb_SHAQKYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44191:SF4">
        <property type="entry name" value="OS01G0187900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44191">
        <property type="entry name" value="TRANSCRIPTION FACTOR KUA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="103"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="7">
        <location>
            <begin position="103"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="8">
        <location>
            <begin position="106"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="71"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="337"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="7"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="71"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="337"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7734327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="356" mass="38437" checksum="78F2EFF3C3DEF6DD" modified="2024-05-29" version="1">MTRRCSHCSNNGHNSRTCPTRTTTTTTTTGGGVVKLFGVRLTDGSIMKKSASMGNLSALYHPSSPASAAAAAAVSPNPNSPATSDPIDGYLSDDPAHASCSAKRRADRTPWTEEEHRLFLLGLQKLGKGDWRGISRNYVLSRTPTQVASHAQKYFIRQCSATRRKRRSSLFDMAPDMAADTPEDHFMLPPAAGTDHSDNCSLKLSLKPDSDQCVEASTSEVIKENIKEETSKPVNELPPIIPAFYPAYIPLPFPVWPSTMAQFDESANYDNNIGPDASHHRVVKPIPILSKEPMNLDELVGMSKQLSLKDPEHPHSIEPSPLSLKLLAEPSRQSAFHASTPVIGSSSVNKGGGGAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C3U5</accession>
    <name>A0AAD5C3U5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_026330</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009675">
        <property type="protein sequence ID" value="KAI7734642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C3U5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7734642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="48" mass="5488" checksum="61345A9EF77202EA" modified="2024-05-29" version="1">MEKKIISAVVMIPAFHCNPTSPNMMTMKESSRRIPRPLMVIMGKFSHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C706</accession>
    <name>A0AAD5C706_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_016070</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009276">
        <property type="protein sequence ID" value="KAI7736397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C706"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37706">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37706:SF2">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="116"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="97"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7736397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="151" mass="17136" checksum="81323505B52944F9" modified="2024-05-29" version="1">MHHFLTTENIRYPRMAPTTLLSPATVNHHHHHSKPPILTTTTHLVPSVHCSCSTSKTTPSHRWSLLPPRTSKLRRRRCRAASPHPPSPPESEPPPGDDQSSSSGSIASLSRFQESVQIFCAVFFWMSLFFWSSVWDGRNNGRSDKGPRFWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C8I3</accession>
    <name>A0AAD5C8I3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_032336</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009168">
        <property type="protein sequence ID" value="KAI7736905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C8I3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006869">
        <property type="entry name" value="DUF547"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025757">
        <property type="entry name" value="MIP1_Leuzipper"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23054">
        <property type="entry name" value="TERNARY COMPLEX FACTOR MIP1, LEUCINE-ZIPPER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23054:SF83">
        <property type="entry name" value="TERNARY COMPLEX FACTOR MIP1, LEUCINE-ZIPPER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04784">
        <property type="entry name" value="DUF547"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14389">
        <property type="entry name" value="Lzipper-MIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ternary complex factor MIP1 leucine-zipper" evidence="3">
        <location>
            <begin position="12"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="DUF547" evidence="2">
        <location>
            <begin position="317"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="6"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7736905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="530" mass="60235" checksum="96F6A2D4647F9970" modified="2024-05-29" version="1">MVQERRFESIEVKKSLKQEIEQLQKQLEDQLTIRSELEKATTSQPFFQDTVDEASLTKSSKDLIKEISILEFEVKHLEKYLLSLYRKTFQKKEQPVSATDTKSRLNSALKEQQLLSGNSANFMPARTSVDNPPKECFPILESQPMEDSNVNRSHSSLSYRTPPLYMAANQAVESYHSLPLGMLEFAKDDHSSVSLAEHLGGCITDNVRMSANCLSEEMIKCISGIYGQVADPPLFNHEFPSSPISFPSPPSDSSPIDQFSMWSPHCEGSMEFSGPYFTTLEVQGLCKSTQRLSSVEHKQQNFRQVSLIVQLEHVDPRKLKHEEKLAFWINIHNALVMHAYLVHGTPRGALKRISLVQKAAYNIGGHNLSVGDIQSTILGCRLPHPGQWFQSLLFPSPKYKSRDARKAYAMKHPQPLAYFALCSGSHSDPMVRVYTPKSVFQELEIAKEEYVHNNFNIQKGQKIFLPKLVDLYAKESGLCHSGLMDMIEYSVPDCYQDSFKSIKKGKSLKKFEWVPHDFTFRYLLSPDLAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C8J3</accession>
    <name>A0AAD5C8J3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_003523</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010009253">
        <property type="protein sequence ID" value="KAI7736523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C8J3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16664">
        <property type="entry name" value="RING-Ubox_PUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000442">
        <property type="entry name" value="RING-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058678">
        <property type="entry name" value="ARM_PUB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057623">
        <property type="entry name" value="PUB12-19-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045210">
        <property type="entry name" value="RING-Ubox_PUB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003613">
        <property type="entry name" value="Ubox_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23315:SF342">
        <property type="entry name" value="RING-TYPE E3 UBIQUITIN TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23315">
        <property type="entry name" value="U BOX DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25598">
        <property type="entry name" value="ARM_PUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25368">
        <property type="entry name" value="PUB10_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04564">
        <property type="entry name" value="U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00504">
        <property type="entry name" value="Ubox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51698">
        <property type="entry name" value="U_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="U-box" evidence="8">
        <location>
            <begin position="270"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="7">
        <location>
            <begin position="405"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="7">
        <location>
            <begin position="446"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="7">
        <location>
            <begin position="487"/>
            <end position="522"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7736523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="585" mass="64347" checksum="2CAF6A07D0E68B21" modified="2024-05-29" version="1">MISHPFYKPTTITQSITTTTMPGDDSTAVVAAIQSLLRLVRDIARRSATGFTGAFKKDCSDMCRRVALLSHLFEEIRDYQGDLRPLDMSASSSSSSNSSSSLSELTQALKGAKKLVLFAGSFDNNNNNMSTDGTAQQIVFQFQCITWKLEKALASLPYDSFDISEEVKEQVDLVRVQLKRAAERYGRSQDLTVLSGGVSESLAKESDILHGVCYDHDEVESKVGNVSNESIVNDVDEMMYEPATGSSEASCLLKPSDDAVNSVEETKKPVIPDDFLCPISLELIRDPVIVSTGQTYERSYIQRWINCGNTTCPKTRQKLQNLILTPNYVLRSLITQWCKLHNVDHPTLLTNSRLKNVDKALLDVIDAIVRNLSSQSIEEQRAAVSEIRSLSKRSTDNRILIAEAGAIPILVNLILSKDNITQQHAVTSILNLSIYEDNRELIMLANAVPSIVQVLRTGAMETRENAAATLFSLSLADENKMIIGASGAIPLLVHLLENGSKRGKNDAATALFNLSANSRNQENAISILLSLCKRDDENLGIISRLGVLTPLMELANNGTERAKRKANLLLGHLQRSQQQLQHVDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C8P0</accession>
    <name>A0AAD5C8P0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_007375</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009072">
        <property type="protein sequence ID" value="KAI7737282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C8P0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7737282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="190" mass="22228" checksum="E865C7827836E6CF" modified="2024-05-29" version="1" fragment="single">AFYLPTHGFIGLQLQVTTSNTWILLKVHHLHCVLSIYRLPLPTNPPFTVPLLTRIDATGVYIPHKRDHQPKSPTNCRPCNEFYVVVQYLTFKWVNTYILKFKVPHLHPYLLISNNEICWLKALPAMQITEPTDPQVTVQELDLAKQQRNKTLYPEEDESLTFVCKDGGRRRAKLQRLHKKHKRHLFLTKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C9L7</accession>
    <name>A0AAD5C9L7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Dynein light chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_002843</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cytoplasm</location>
            <location evidence="1 8">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the dynein light chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009024">
        <property type="protein sequence ID" value="KAI7737445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C9L7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005868">
        <property type="term" value="C:cytoplasmic dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21452">
        <property type="entry name" value="DLC-like_DYNLL1_DYNLL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.740.10:FF:000001">
        <property type="entry name" value="Dynein light chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.740.10">
        <property type="entry name" value="Protein Inhibitor Of Neuronal Nitric Oxide Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037177">
        <property type="entry name" value="DLC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019763">
        <property type="entry name" value="Dynein_light_1/2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001372">
        <property type="entry name" value="Dynein_light_chain_typ-1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11886">
        <property type="entry name" value="DYNEIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11886:SF35">
        <property type="entry name" value="DYNEIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01221">
        <property type="entry name" value="Dynein_light"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01375">
        <property type="entry name" value="Dynein_light"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54648">
        <property type="entry name" value="DLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01239">
        <property type="entry name" value="DYNEIN_LIGHT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5 8" id="KW-0243">Dynein</keyword>
    <keyword evidence="4 8" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6 8" id="KW-0505">Motor protein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="39"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU365010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7737445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="138" mass="15521" checksum="EAFE248450B226D5" modified="2024-05-29" version="1">MPFFTPHPNQMMSEDRNRSLAGVMPPAKPMPSVDDRKTSASPPSISSSTSINRKIVIKSADMKEEMQTEAVNIAIKAFENLTVEKDVAEQIKKEFDKNHGPTWHCIVGKNFGSYVTHETNHFVYFYLDSKAVLLFKSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C9P2</accession>
    <name>A0AAD5C9P2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_000490</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009257">
        <property type="protein sequence ID" value="KAI7736506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C9P2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7736506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="81" mass="8828" checksum="7927350E6D525B52" modified="2024-05-29" version="1">METSLKGLASVTVVTVGVIIEDNLGFHHEPLAATMVFQGSRKVQGIVYTGNGKGMMSATPWFYLQRDSKVGHEKDPEGLYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CA63</accession>
    <name>A0AAD5CA63_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_007549</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008860">
        <property type="protein sequence ID" value="KAI7738166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CA63"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043136">
        <property type="term" value="F:sn-glycerol 3-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006114">
        <property type="term" value="P:glycerol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07505">
        <property type="entry name" value="HAD_BPGM-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000119">
        <property type="entry name" value="Bifunctional riboflavin kinase/FMN phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="entry name" value="Putative phosphatase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01549">
        <property type="entry name" value="HAD-SF-IA-v1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01509">
        <property type="entry name" value="HAD-SF-IA-v3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18901">
        <property type="entry name" value="2-DEOXYGLUCOSE-6-PHOSPHATE PHOSPHATASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18901:SF44">
        <property type="entry name" value="OS01G0757900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00413">
        <property type="entry name" value="HADHALOGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7738166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="245" mass="27271" checksum="657AA813363D4755" modified="2024-05-29" version="1" fragment="single">GLVSEVLKVYLVKHKKKWDGREVHKIVGKTPLEAAAVIVEDYALPLSKEDFLSEFSPIWCKLKAQPGAYRLIKHLRGHGVKMALASNSPRANIDIKLSYHLTLKESFAAIVASDEVKEGKPSPEIYLEAAKRLNIHPSKCLVIEDSQPGICAAKAAKMEVVAVPSIPNQPHLYTAADEVIISLLDLRLETWGLPPFEDIANFPSLIHEDGELAEKTLELPLYAKYNDDEYFKSIPAPDHNNHLSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CAN2</accession>
    <name>A0AAD5CAN2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ribulose bisphosphate carboxylase small subunit, chloroplastic</fullName>
            <shortName evidence="10">RuBisCO small subunit</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">RBCS</name>
        <name evidence="14" type="ORF">M8C21_025154</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10 12">RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate. Both reactions occur simultaneously and in competition at the same active site. Although the small subunit is not catalytic it is essential for maximal activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10 12">Heterohexadecamer of 8 large and 8 small subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell junction</location>
            <location evidence="9">Plasmodesma</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Plastid</location>
            <location evidence="10">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="10">The basic functional RuBisCO is composed of a large chain homodimer in a 'head-to-tail' conformation. In form I RuBisCO this homodimer is arranged in a barrel-like tetramer with the small subunits forming a tetrameric 'cap' on each end of the 'barrel'.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10 12">Belongs to the RuBisCO small chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008967">
        <property type="protein sequence ID" value="KAI7737720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CAN2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009506">
        <property type="term" value="C:plasmodesma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016984">
        <property type="term" value="F:ribulose-bisphosphate carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009853">
        <property type="term" value="P:photorespiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019253">
        <property type="term" value="P:reductive pentose-phosphate cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03527">
        <property type="entry name" value="RuBisCO_small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000184">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.190.10:FF:000001">
        <property type="entry name" value="Ribulose bisphosphate carboxylase small chain, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.190.10">
        <property type="entry name" value="Ribulose bisphosphate carboxylase, small subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00859">
        <property type="entry name" value="RuBisCO_S_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024681">
        <property type="entry name" value="RuBisCO_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000894">
        <property type="entry name" value="RuBisCO_ssu_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036385">
        <property type="entry name" value="RuBisCO_ssu_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF350">
        <property type="entry name" value="(WILD MALAYSIAN BANANA) HYPOTHETICAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00101">
        <property type="entry name" value="RuBisCO_small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00152">
        <property type="entry name" value="RUBISCOSMALL"/>
    </dbReference>
    <dbReference type="SMART" id="SM00961">
        <property type="entry name" value="RuBisCO_small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55239">
        <property type="entry name" value="RuBisCO, small subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0113">Calvin cycle</keyword>
    <keyword evidence="8 10 12" id="KW-0120">Carbon dioxide fixation</keyword>
    <keyword evidence="1 10" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="7 10 12" id="KW-0601">Photorespiration</keyword>
    <keyword evidence="2 10 12" id="KW-0602">Photosynthesis</keyword>
    <keyword evidence="4 10 12" id="KW-0934">Plastid</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="repeat" description="PPR" evidence="11">
        <location>
            <begin position="56"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="11">
        <location>
            <begin position="154"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="11">
        <location>
            <begin position="185"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="11">
        <location>
            <begin position="287"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="Ribulose bisphosphate carboxylase small subunit" evidence="13">
        <location>
            <begin position="563"/>
            <end position="671"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7737720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="674" mass="75472" checksum="199DD2D06ADCE48B" modified="2024-05-29" version="1">MLEKCSTMDQLKQIHAQTITAGFARFTYITSKLLAFSAIYDVHYAHTVLNRISMPTIFDFNTVILGYSNTSNQEMGILLYTKMRNKGIKPNDRTFPVLIKTCDCLRSLVQVHGQVVKHGNVSDVYLTSSLICMYSDYKAIELAKQVFEESSYRNVVCCTSLITGCFSNRLVDDACKIFEEMPDRNDVSYSAMISGYVKNELFNEAIQLYRQMMNCGLGSANRSLLLSILNACGAVGAFDLGSSIHCQLVDDNSSVDLDFGTTLIDFYAKCGYIEKAEDIFNAMPRKDVAAWSAMILGLATNGKSEAAINLFEEMEQKGPVPNGITFVAVLVACNHGTLLKKPWSILGKMSKVYNVELTIEHYGCMIDLLARSGQLKEAEALIKLMPMTPDGAIWGSFLHGCLTHNEIHLAEKAGKHLLELEPKHSGRYVGLANMYANNASWDGVIKLRKMMTERKVAITPGWSFIEVNGIVNKFFVDDQCHPQAREIHDVLSSIVIPFSTTIGSRMSSATFAAPVTGSMYVGLNPNGSKLFQTANSVPWSRKTVSNGSRIHCMKVWNPINNKKFETLSYLPPLTDESIAKEIDYMIKKGWVPCLEFDLVGHVFRENSQIPNYYDGRYWTLWKLPMFGCSDSSQVLNEIHECKKTYPNAYIRCLAFDNVRQAQCMSFVIQKPTDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CAZ1</accession>
    <name>A0AAD5CAZ1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CRAL-TRIO domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_008415</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008822">
        <property type="protein sequence ID" value="KAI7738352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CAZ1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48411">
        <property type="entry name" value="OS01G0948300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48411:SF1">
        <property type="entry name" value="OS01G0948300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13716">
        <property type="entry name" value="CRAL_TRIO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="1">
        <location>
            <begin position="13"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7738352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="197" mass="23228" checksum="25D2527CD8B6B532" modified="2024-05-29" version="1">MAEKPQQPDHIQLIHNLNLFKIKGRDKRGRKILKIIGKHFPAKSLNADMLKKYLEVKIFPKVERPFVVVYIHTDVHKSYNFPGFSVLRCVYDAIPINVKQNLESVYFIHPDLQSRVFLATFGRFIFTGGLYGKVKYVSRLSYLWDHVKRNEIEIPEFVYDHDDDLDYRPMMDYGLESDHPRVYDAPPVSMYSMRCIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CB02</accession>
    <name>A0AAD5CB02_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_014514</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008871">
        <property type="protein sequence ID" value="KAI7738105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CB02"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009395">
        <property type="term" value="P:phospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007312">
        <property type="entry name" value="Phosphoesterase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31956">
        <property type="entry name" value="NON-SPECIFIC PHOSPHOLIPASE C4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31956:SF28">
        <property type="entry name" value="NON-SPECIFIC PHOSPHOLIPASE C4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04185">
        <property type="entry name" value="Phosphoesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7738105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="330" mass="37730" checksum="6894AD0E6F7383D4" modified="2024-05-29" version="1" fragment="single">QPDPGHSFQAIYQQVFGVPWTEESSSSTMTPTMEGFAEQAESVEEGLGQVVMSGFKPDDVPVYRDLVKEFAVCDRWFASVPASTQPNRLYVHSATSHGLISNDTLKLIEGFPQKTIFESLEESGFSFGIYYQYPPTTLFYRNLRKLKYISKFHQFDLDFKRHCKEGKLPNYVVVEQRYFDLKILPANDDHPSHDVSEGQKFVKEVYEALRSSPQWNEILFLVIYDEHGGFYDHVPTPTVGVPSPDGIVGPEPYKFKFDRLGVRVPAILISPWIERGTVLHEPAGPFPTSQYEHSSISATVKKIFNLKEFLTKRDEWAGTFEGLHYLNQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CBH3</accession>
    <name>A0AAD5CBH3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_008452</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008822">
        <property type="protein sequence ID" value="KAI7738389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CBH3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019341">
        <property type="entry name" value="Alpha/Gamma-adaptin-bd_p34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14659">
        <property type="entry name" value="ALPHA- AND GAMMA-ADAPTIN-BINDING PROTEIN P34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14659:SF1">
        <property type="entry name" value="ALPHA- AND GAMMA-ADAPTIN-BINDING PROTEIN P34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10199">
        <property type="entry name" value="Adaptin_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="222"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="236"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="269"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="280"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="293"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7738389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="381" mass="42600" checksum="B78804C3BAA7D891" modified="2024-05-29" version="1">MDDRDTLSKRPGVLLLGSSGVGKRTLLSRLLSVDFEDEADSSSDIHAYGWTINTKYYAADISVWMAHLNNEFQITVAPMFDNLAALVMVFDLNNLSSFSELKKWVSCNNIENFDILLCIGNKADLVPHHSGHVEYRRRLLNLDESADYGISETEGSYLLGDEESSSEIKISCMEWCLEHNIEYIEACASNPRFDKCLSVDGDSQGVDRLLGALSAHMWPGMVLKSGDNTNQPSLPQEEDTSDEEEPNYELEYDILSGGSVDPWDDTDVSWVSATNNNTEPDIKNDRLAGEDMQPSTSQSQSQENEIKRERAITPVAHDEANDEGGMHFELEDMEQLMSEIGSMRDSLRLMPGFQRRKMAATLAMKMASMFGDTSGDDEEEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CBQ2</accession>
    <name>A0AAD5CBQ2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_009776</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008846">
        <property type="protein sequence ID" value="KAI7738245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CBQ2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009506">
        <property type="term" value="C:plasmodesma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004714">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.1190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045272">
        <property type="entry name" value="ANXUR1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27003">
        <property type="entry name" value="OS07G0166700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27003:SF383">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE, NON-RECEPTOR JAK_TYK2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="28"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="73"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="4">
        <location>
            <begin position="28"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7738245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="418" mass="47787" checksum="77D36D95511132F1" modified="2024-05-29" version="1" fragment="single">MSQSDPVTDQTGLEEPGMGSGSGSYPQRPDEPDCSYYIRTGFCRFGSRCRFNHPPGRALRYPLKDILSATSNFSRENLIAECALGRVYKGQFQRTKLVVQRLDCKNGQGDKLQAEILMIKSLKHKNIVSVYGYCDENNEKIIIYKTIYGTLSQHLSDQILTWSQRLQICLGVARGLNYIHYDVIHCDINSSKIILEENWEPKIYGFELSTKYPQSWRHRLLFSRYFDTNNLTPKYDVYSFGVLLLEVICGRKPLITNDGIQEELDEIIDPNLRKQMDSQTLAHFTNIAYKCLSQQHVQRPTMDQVVKDLEEVMELHWEHADLKEQHSKAADEATSSNNLKGETVVLLRFRLSDIVFATENFAETYRIGLDNIGTVYKAELDHFDSNSSLATEGRCDGEPSKNRISVAIKRITSREGGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CC98</accession>
    <name>A0AAD5CC98_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_030545</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008687">
        <property type="protein sequence ID" value="KAI7739012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CC98"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046956">
        <property type="entry name" value="RLP23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063:SF103">
        <property type="entry name" value="LEUCINE-RICH RECEPTOR-LIKE KINASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063">
        <property type="entry name" value="LRR RECEPTOR-LIKE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7739012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="260" mass="29526" checksum="5408C3BC6B3A84BE" modified="2024-05-29" version="1" fragment="single">ITSATSLGHQLAAANGDGNYECIDKERRVLHFKADICQDCFGLLSSWTREKKQQLIVVIGKESHATVATPKQTIPLEFGNITNIQDLYLEDLGGCTLENLDWLSRLSQLEKLCMSDLYNNHKGNQFKGSLLDDIQKFSSLVYLDLSNNQLNGTISEKVWELSDLQLLYLSSNFLRCVISKNIGKSKTWSLHLSNNSFEGVPFDVHISNHSYIEYISLRSCKLGPSFSKWIWKLKELAYLDISDTSILDTIPQEFWNMSTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CCJ1</accession>
    <name>A0AAD5CCJ1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_026363</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008875">
        <property type="protein sequence ID" value="KAI7738094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CCJ1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000001">
        <property type="entry name" value="Calmodulin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039647">
        <property type="entry name" value="EF_hand_pair_protein_CML-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10891">
        <property type="entry name" value="EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="36"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="74"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7738094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="108" mass="12677" checksum="BCD30EA2295074C3" modified="2024-05-29" version="1">MVMGNLGIFYNSKGENNLPRSLSSDDLFNIFEQEHPRLDEVREAFDVFDENKDGYIDARELKRVFSALGLRDKVNMDDCKKMIRVYDDNNDGRIDFDEFVKFMEGTFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CD54</accession>
    <name>A0AAD5CD54_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">M8C21_000493</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">AA19_3_7</strain>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010008574">
        <property type="protein sequence ID" value="KAI7739727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CD54"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12122">
        <property type="entry name" value="AMPKA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14079">
        <property type="entry name" value="STKc_AMPK_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000003">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001222">
        <property type="entry name" value="Serine/threonine-protein kinase ppk25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.80">
        <property type="entry name" value="Kinase associated domain 1, KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028375">
        <property type="entry name" value="KA1/Ssp2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001772">
        <property type="entry name" value="KA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF82">
        <property type="entry name" value="KP78A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02149">
        <property type="entry name" value="KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103243">
        <property type="entry name" value="KA1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50032">
        <property type="entry name" value="KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 11 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="15"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="KA1" evidence="14">
        <location>
            <begin position="442"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAI7739727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="490" mass="55588" checksum="6A26BCFF14581A2D" modified="2024-05-29" version="1">MDDNGSGSLPPLSNYRLGKTLGFGAFGKVKVAQHVLTGIKVAIKILDRQSINDSDGERVRREIKIMRLLSHPHIVRLYEVIETRSTIYVVMEYMNSGELFDYITENRRLEENHARHFFQQIISGVESCHLHMVVHRDLKPENLLLDSKGNVKVADFGLANVMRDGHLLKTSCGSPNYASPEVISGHLYAGPEVDVWSCGVILYALLSGGLPFDDEIMTVLLAKIKSGIFTCPRYFSHGARDLIRRMLNVDPVNRITIPEIYKHPWFQINLPRYIGDHSINTSWSARKVDVRVLEEMDILGFNVQEVIGSLNNLWQNQATVSYSILLHRRLNNLSRQEDNHLGSLELEGTDRRDIYVRPTFPVQGKWVLGFKSCSSAHETMRDVLTVFKRLNVQWKKIGPYNVKCLWKPAVQRCSLPMGINGITLREDTNGVSAPLNTSNNETSSFCDDVKFEIQLYKGSAETYVLDWQRVYGAPFLFIEVCAAFRACVIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CDW0</accession>
    <name>A0AAD5CDW0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_006261</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008719">
        <property type="protein sequence ID" value="KAI7738814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CDW0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7738814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="62" mass="6985" checksum="80F43E27EDD602C0" modified="2024-05-29" version="1" fragment="single">VHQFVTRRLEQFLIGDGSDEIKVRRAVVEVLITRKCSCGEDAVGYSITVMVMQQWNGSEEIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CEF8</accession>
    <name>A0AAD5CEF8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_003686</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the major facilitator superfamily. Phosphate:H(+) symporter (TC 2.A.1.9) family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008424">
        <property type="protein sequence ID" value="KAI7740462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CEF8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015145">
        <property type="term" value="F:monosaccharide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17361">
        <property type="entry name" value="MFS_STP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000002">
        <property type="entry name" value="Sugar transport protein 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044778">
        <property type="entry name" value="MFS_STP/MST-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045262">
        <property type="entry name" value="STP/PLT_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23500">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 2, FACILITATED GLUCOSE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23500:SF30">
        <property type="entry name" value="SUGAR TRANSPORT PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="6" id="KW-0769">Symport</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="111"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="136"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="169"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="199"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="282"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="316"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="347"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="381"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="419"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="451"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="12">
        <location>
            <begin position="25"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7740462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="515" mass="56453" checksum="E77AE271611321BB" modified="2024-05-29" version="1">MAVGSEVSSEVVKYNGHITSFVVLSCMVAATGGIIFGYDIGISGGVTSMEPFLKKFFPEVYTKMKKDTNISNYCKFDSQLLTSFTSSLYVAGLVSTFFASPVTRAFGRRPSILIGGAAFLAGAALGGAAYNIYMLIIGRVLLGVGVGFANQSVPLYLSEMAPSQYRGAFNMCFQLCVGIGVLAANLLNYGTEKIEGGWGWRISLAMAAVPASILTLGTFFLPETPNSLIQHNPDKAKQMLQKIRGTDNIQAEFNDLTTANETSKHIKHPFKNILRRKHRPQLVMAILIPFFQQVTGINVISFYAPVLFRTIGFGESASLMSAIVTGLVGLCMTFLSLLIVDKLGRRTIFTIGGVQMFVSQILVGTIMAAKLGDHGGLSKGYGYLVLILICSYVAGFGLSWGPLGWLIPSEIFPLEIRSAGQSITVAVGFLFTFIVAQTFLTMLCHMKSGTFFFFGGWVAVMTVFVYLFLPETKNVPIETMDRIWKQHWYWKRYVSEEDDMEIDSDPTGLVKQRKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CGN0</accession>
    <name>A0AAD5CGN0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_023923</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PspA/Vipp/IM30 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008404">
        <property type="protein sequence ID" value="KAI7740555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CGN0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007157">
        <property type="entry name" value="PspA_VIPP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31088">
        <property type="entry name" value="MEMBRANE-ASSOCIATED PROTEIN VIPP1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31088:SF12">
        <property type="entry name" value="MEMBRANE-ASSOCIATED PROTEIN VIPP1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="46"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00043985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7740555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="87" mass="9655" checksum="0B0923D7F714FBC2" modified="2024-05-29" version="1">MEEKVMSMEFQAEALNQLSTDDLDGKFAMLESSSVDDDLASLKKELSGSTKKGDLPPGRTSSKAAYPFPDADIENELNELRQRTRDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CHY8</accession>
    <name>A0AAD5CHY8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UDENN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_018599</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007993">
        <property type="protein sequence ID" value="KAI7742398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CHY8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11500">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001194">
        <property type="entry name" value="cDENN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043153">
        <property type="entry name" value="DENN_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051942">
        <property type="entry name" value="DENN_domain_containing_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037516">
        <property type="entry name" value="Tripartite_DENN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005113">
        <property type="entry name" value="uDENN_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15288:SF30">
        <property type="entry name" value="CDENN DOMAIN, UDENN DOMAIN, TRIPARTITE DENN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15288">
        <property type="entry name" value="DENN DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02141">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03456">
        <property type="entry name" value="uDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00799">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00800">
        <property type="entry name" value="uDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50211">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDENN" evidence="2">
        <location>
            <begin position="192"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="418"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="461"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="479"/>
            <end position="489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7742398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="782" mass="88140" checksum="3B9C9780F30B367D" modified="2024-05-29" version="1">MVKDKEENNESDRDRERWMQVFLILMERKGRDVGVGGSIAWLFKEDRALMDKEDPNTSSPGWGASFLVHHTTTTDDPPSPAPRPSVVFSSKGGDSSGSPLQKLQSQVFRVLKGFSPPPEDKTRTYNPEVLTTQKRQWASFQLQALHQRILKEPSRLFESIVVVGLHPNCDIQALQRQYFGRRSELPGRLRTALSVQNQFRVEPNLEPQVLFVYPPDKQLPLRSKDLLSFCFPGGLEVHTIERTPSMSELNEILLGQEHLKQNDLSFVFRLQGADDSILYGCCVLVDELVQQPSRLISMMGDRQLSSPPMSRHILTTRRCYCILTRLPFFELHFGVLNSIFIEERLERLTKSISDLDVELVYDNEVYSEDKVSGGSAEYGSQELLNGTTETVQLIRSNSITERVGDCVIPVRPEIEIDSSKKECESPPVDDMVSNKHNTQKQIPNAILPLLRYQQYDSSDSSSRYFHLGQPKIGRSRGSPSEDRSFRSDVDSPETEEASSSGQEDNEHNEILNWAKTSNHGSLQIICEYNRLCVPARGSTIKFHPLDHLHPLEFHRPDETVLHIAGSKVDLMSCSTSFELAEAHKALAVEEEATALSIWAISSICGSLRLEHILTIFAGALLEKQIVFVCSNLGILSASVLSIIPLIRPYQWQSFFMPVLPNDMLDFLDAPVPFIVGVKSKTAEVQSKSGNVILVDANKNQVKSLSIPHFPRYKELYAALTPYHAQLVGESYLGKRRPVYDCTDVQVEAAKGFLRVSLLLKESFIESFPSRDRPFMKHPVCGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-02-05" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CJV7</accession>
    <name>A0AAD5CJV7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_030668</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. BUD32 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010008061">
        <property type="protein sequence ID" value="KAI7742175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000408">
        <property type="term" value="C:EKC/KEOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070525">
        <property type="term" value="P:tRNA threonylcarbamoyladenosine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001708">
        <property type="entry name" value="Protein kinase superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000201">
        <property type="entry name" value="TP53-regulating kinase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022495">
        <property type="entry name" value="Bud32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03724">
        <property type="entry name" value="arch_bud32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12209:SF0">
        <property type="entry name" value="EKC_KEOPS COMPLEX SUBUNIT TP53RK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12209">
        <property type="entry name" value="NON-SPECIFIC SERINE/THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="33"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7742175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="250" mass="28033" checksum="B016A4D443B567A7" modified="2024-05-29" version="1">MLHYYSIDDAMLGQHKHQEVEXXXXDTETNGNNTSLVLIKQGAEARVFESTFAGRKSIVKERFSKKYRHPTLDSKLTLKRLNAEARCMTKARRLGVSTPVLYAVDPISHTLTFEYIEGPSIKDIFLEFGSEGVVEEKMDDIAMQIGDTIGKLHDGGVIHGDLTTSNMLWRSGSNQLVLIDFGLSFVSTLPEDKAVDLYVLERALLSMHSSCGNVMDKILAAYRKSSKQWSATFNKLGQVRLRGRKRTMIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CKG3</accession>
    <name>A0AAD5CKG3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_011246</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007732">
        <property type="protein sequence ID" value="KAI7743483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CKG3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36376">
        <property type="entry name" value="OS09G0514700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36376:SF1">
        <property type="entry name" value="OS09G0514700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7743483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="322" mass="36062" checksum="242B07A506FB17CF" modified="2024-05-29" version="1">MYIQAPGAELVNSSIRKSLRLQSKKGLSSYSKYVFKSYHSNSKCTNDSSKTSLCKENSVSEGKIQEEKKHQFLHRNEVNGAFSANTVSMKSSTVVPSFEYHVQAKDGIRLVVDLNMKRSDWLKSMGKTVCVCQDHSKPAFESFRKEVEGLRDRNNLKVSSPDKNAASDASMSSCVENKFSVESTSRETEKTLPSDVEKEVITLSGSKQCWSKVDQNSELVPGSFENSEEVQLSDFSSPQKALLHVQERIKSAEGIEGSGREENAFVTSASDDEPKRKKRYYKSDHIHGQSHERILRSTLGGKVLEHDGVSVWRSSRLHSKVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CL37</accession>
    <name>A0AAD5CL37_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein bL28m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_012054</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL28 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007707">
        <property type="protein sequence ID" value="KAI7743632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CL37"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.170.40:FF:000003">
        <property type="entry name" value="54S ribosomal protein L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.170.40">
        <property type="entry name" value="Ribosomal protein L28/L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00373">
        <property type="entry name" value="Ribosomal_bL28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026569">
        <property type="entry name" value="Ribosomal_bL28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034704">
        <property type="entry name" value="Ribosomal_bL28/bL31-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037147">
        <property type="entry name" value="Ribosomal_bL28_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13528">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L28, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13528:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN BL28M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00830">
        <property type="entry name" value="Ribosomal_L28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143800">
        <property type="entry name" value="L28p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="186"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="195"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7743632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="206" mass="23856" checksum="FDF06C0B592759F6" modified="2024-05-29" version="1">MAFRSKEMVKKIMKKIGGEKNMGPGVKESLKKSLPDSKVVMGRAHRGIYAGRHIQYGNQVSEKGGNKTRRNWKPNVQDKRLFSYILDRHIRVKVTTHALRCIDKAGGIDEYLLKTPYKKMDTEMGLLWKSKIEKMYQELGNMEVLFFPQEEENKLAEEFKEMRIEQRVARRDARRKAYGWSPKAEEIEGVSEDGEASHHDELVANS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CL90</accession>
    <name>A0AAD5CL90_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Inactive TPR repeat-containing thioredoxin TTL3-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_014973</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007699">
        <property type="protein sequence ID" value="KAI7743707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CL90"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02947">
        <property type="entry name" value="TRX_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044534">
        <property type="entry name" value="TTL1-4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46050:SF30">
        <property type="entry name" value="TETRATRICOPEPTIDE-LIKE HELICAL DOMAIN, THIOREDOXIN-LIKE FOLD PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46050">
        <property type="entry name" value="TPR REPEAT-CONTAINING THIOREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13414">
        <property type="entry name" value="TPR_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="214"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="411"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="23"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="23"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="57"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="95"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="167"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7743707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="679" mass="73725" checksum="ED5AE9A1A74593EC" modified="2024-05-29" version="1" fragment="single">MSHPINSISDRFKHSVTCHGDDSNKPDFHELDLDPGHLPGHVSVSGPGSCSPISPLRVGPTISSSSSSSGSVSGRIGGTSNNNNTNNHHSGELTVDSSPTFSVNGSKPKPGHTHTHTRSESSWSSATSPPPVNVLPAGNICPSGRVLKTGMMTNRSSKPEVLSLGSGNYGHGSIMRGGSGSGSGSGSGSGKVETRVPVGSGGSGLGSRRGLMDPEEVKRLGNEEYKKGHFLEALRLYDKAIVLSPANAAYHCNRAAALIGLKRLSEAVKECYEAIRLDDGYVRAHHRLGSLLVSLGQVENARRHLYFKEFQPDPNEAQKLQLVEKHLNKCTDSRRVRDWGNVLKETDSAIVSGADACAQLFACKAEALLKLHKLDDADSVISHLPKVETCSSSSSSLSCSQVKFFGMQSEAYVLFVRAQIDMSLGRFENALTSIEKAGQIDPRSVEVALLLQNIRSLCRSRARGNELFKSERFTEACSAYGDGLKCDPSNPILYCNRAACWFKLGQFERSLDDCNHALLVHPSYTKALLRRAATFSKLERWAESVRDYEVLRRELPNDNDIAESLFHAQVALKKSRGEEVYNLKFGGEVELINSLEQFKAAVASTGASVVLFKTASDLQCKQISPFLDTLCKRYPSINFLKVDIEESQAIASAENRWLSVDEGQNCNFSTLQSSMEGVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CLR9</accession>
    <name>A0AAD5CLR9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CUE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_010410</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007852">
        <property type="protein sequence ID" value="KAI7742939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CLR9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038981">
        <property type="entry name" value="CID5/CID6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003892">
        <property type="entry name" value="CUE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37252">
        <property type="entry name" value="POLYADENYLATE-BINDING PROTEIN-INTERACTING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37252:SF3">
        <property type="entry name" value="POLYADENYLATE-BINDING PROTEIN-INTERACTING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02845">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51140">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CUE" evidence="2">
        <location>
            <begin position="86"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="136"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="148"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7742939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="179" mass="19015" checksum="33F9B181E0F41571" modified="2024-05-29" version="1">MKARSSSLNPYATSYIPLSRRGATDEGKSNDHGSTTLNQSPKYSENHDAVSESPVSLKLNNQSGLGSYGSSSHSAELAGKQAMDIDHDMNLAYLQMVFPGVSDESLSSVYTACNGDIEAAVEMLSQLELHSGDFTENLPDSLDIGDVSEAGSSSEGGSQKLKKVVVAEGSWWNQTPPPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CN71</accession>
    <name>A0AAD5CN71_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">SANT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_017454</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007425">
        <property type="protein sequence ID" value="KAI7744857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CN71"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056067">
        <property type="entry name" value="DUF7650"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057712">
        <property type="entry name" value="DUF7952"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13859">
        <property type="entry name" value="ATROPHIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13859:SF11">
        <property type="entry name" value="GRUNGE, ISOFORM J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24662">
        <property type="entry name" value="DUF7650"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25826">
        <property type="entry name" value="DUF7952"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="DUF7952" evidence="7">
        <location>
            <begin position="87"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7650" evidence="6">
        <location>
            <begin position="255"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="470"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="572"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="510"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="577"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="603"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="671"/>
            <end position="681"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF24662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF25826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7744857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="681" mass="76096" checksum="60FF1E61A8021DCC" modified="2024-05-29" version="1">MDPNDDTDNSPLETYAYGLYGPIGTLYHIHVGDEYQAIIPSLMTHSEQNTAVSINFSIGLDIPVVWNNKHNMKNGNIPVPGHVLESWSEVEKGSFVLGLYIFRKDFVRLSRFIESKTVGDILAYYYGSFYRSDEYKRWTGCKKLRGKRCIMGQRIFRGVRQQEFLARLVPKVSQECQNDLLEVSRTFGDAKISLERYVFAIKALIGIENFIDAVAIGKGKQDLTGNVIEPTKKNQAIRIRPETPVGKACSKLTSAEIIQVLTGVYRLSKNRSYELFWEAVWPRLLARGWHSEQPSGYNYAANGKDHLVYLMPGVDKFSRKLVKGEAYFDSLTDVLNKVALDPQLIELDNEENGENETRLEDEEENGLVEKRKSRYLQPRAANVNRGNGVVKFTVVDTSLRSGKIFSVRELDAVKLSLASSSDESRSEMVSSDVSDCENICFVDQKSAKKQKTTDEIKNLEKKDVKTANGLDSIVKKEKSSRKPSQGKIAPSAKKRRRLTACSRVDSKAGSSLDSHVGSSSQANNNNNNHKLSSNLSFTSRCSSIDTVDEQNQPQPKTFIDLNYPHVTPEFENSELVTKVKDEKVELDPPRQSETIAAPKEQENGGSRRQSTRNRPPTARALEAIANGFLTVNSRKGKKNKQELNSRNDQNTPGDVAVVSESSTGDASSAVKGEDENGEIEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CQA2</accession>
    <name>A0AAD5CQA2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_020954</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily. Type-7 methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007435">
        <property type="protein sequence ID" value="KAI7744814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CQA2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005299">
        <property type="entry name" value="MeTrfase_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31009">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE:CARBOXYL METHYLTRANSFERASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03492">
        <property type="entry name" value="Methyltransf_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="147"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7744814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="294" mass="32728" checksum="A8C3DB7C76891201" modified="2024-05-29" version="1" fragment="single">MTTGHEDSSYAKNSLLQKIAIQKAIPALRRTIQSINSHDIFLDSCVTIADLGCSSGPNTLLVVSCIIDIVIEVCKEDNHKPRQFQVCLNDLFGNDFNTLFKLVSDFYAKLKKEKGENFGPCFVSAVPGSFYGRLFPDLSLHLVHSSFSVSMIALLKANIGISLLLFCILTLYNLYLDATRFIILKVPEGIENNALNIYTAKTSPPNVFQAYAYQFRTDFTCFLKMRSKEIVSGGGMVLTFLGRSIDDPTSDDGCSYLEQLALSLRDLVKEGMVRESDINSFNVPTYHPCVNEVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CRP3</accession>
    <name>A0AAD5CRP3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Sugar phosphate transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_033455</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007186">
        <property type="protein sequence ID" value="KAI7745609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CRP3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004853">
        <property type="entry name" value="Sugar_P_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050186">
        <property type="entry name" value="TPT_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11132">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03151">
        <property type="entry name" value="TPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="42"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="74"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="194"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="224"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="256"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="283"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="Sugar phosphate transporter" evidence="6">
        <location>
            <begin position="24"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7745609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="351" mass="38241" checksum="6E5F0C54BD47D786" modified="2024-05-29" version="1">MEMEKRSNGGVSDVGAWAMNVVSSVGIIMANKQLMSSTGYAFTFATTLTGFHFAVTALVGFVSNSTGYTTSKHVPLWELLWFSIVANMSITGMNLSLMLNSVGFYQITKLSMIPVVCVMEWILHNKQYSKEVKTSVLVVVIGVGVCTVTDVKVNAKGFICACVAVFATSLQQISIGSLQTKYSIGSFELLSKTAPIQALSLLIFGPFIDFYLSGNLVSNYMKTISWGAVLFILLSCSLAVFCNISQYLCIGRFSAVSFQVLGHMKTVCVLTLGWILFDSELTFKNIMGMLVAVGGMVIYSWAVEVEKAVMKATSVGKNSLTEEELKLLRDGMDNHPIKDDKLGESKEVLDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CRU2</accession>
    <name>A0AAD5CRU2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">WRKY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_033317</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006825">
        <property type="protein sequence ID" value="KAI7747138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CRU2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.80">
        <property type="entry name" value="WRKY domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003657">
        <property type="entry name" value="WRKY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036576">
        <property type="entry name" value="WRKY_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044810">
        <property type="entry name" value="WRKY_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31282">
        <property type="entry name" value="WRKY TRANSCRIPTION FACTOR 21-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03106">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00774">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118290">
        <property type="entry name" value="WRKY DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50811">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="WRKY" evidence="7">
        <location>
            <begin position="111"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="222"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="224"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7747138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="293" mass="33168" checksum="7EAF704655707FDE" modified="2024-05-29" version="1">MLLDMESNKERLIQTLITGRDSAQKLQTFLQRRLNVDGSVPVKDLLTEILESFSGGLSMLNSSDSGEISRVPASHPVIQIPDVCTGKKPAPVVKERRGCYKRRRTIDSRVKISSTMEDGYAWRKYGQKEILNSKFPRCYFRCTHKHFHGCKAVKQVQKLEGESNMFHITYFGQHTCPPSDSLSHKHEVVLDFEDTKNHNLFPKSPSTITNIHIDPYVKQEADSKTQSTVSDNISSGNDDQSSSALRWNEVLRADLGSFMRFDHEDSSASTMSHGYVDMDLLNNGDFLSDLYSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CTM1</accession>
    <name>A0AAD5CTM1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Polygalacturonase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_028540</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the glycosyl hydrolase 28 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006614">
        <property type="protein sequence ID" value="KAI7748036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CTM1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004650">
        <property type="term" value="F:polygalacturonase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.10:FF:000004">
        <property type="entry name" value="Pectin lyase-like superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000743">
        <property type="entry name" value="Glyco_hydro_28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31375">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00295">
        <property type="entry name" value="Glyco_hydro_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00710">
        <property type="entry name" value="PbH1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00502">
        <property type="entry name" value="POLYGALACTURONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0134">Cell wall</keyword>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6 9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042208758" description="Polygalacturonase" evidence="10">
        <location>
            <begin position="25"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7748036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="394" mass="41705" checksum="657CF9A8E870490B" modified="2024-05-29" version="1">MTNHLLLLITIITTTTIIITETAAAATYNVNKLGAKPDGKTDSTKAFKAAWSRACGSGKPATMYVPQGTYMVGGMQFSGPCKNDNVTIQIDGALMAPSNYLVLAKAQYWLRLQHVQGVNVVGGVMDAQGAGLWDCKSSGSKNCPDGATTLAIFNSKNVNVYGLTSVNSQMFHIVVNGCSNVRVVGVTVEAPRDSPNTDGIHVQLSTGVTILDSKIGTGDDCVSIGPGAMNLWVENVGCGPGHGISIGSLGKDFNEQGVKNVTVKRVNFKNTDNGFRIKSWARSSKGFVDGVLFQNAVMTNVQNPIIIDQNYCPSHKNCPKQVSGVKISNVKYQDVHGTSATKVAVKFDCSKKYPCKGITMQDVNLSFTEEAPASSYCANAGGQTSGVIKPTSCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CTQ8</accession>
    <name>A0AAD5CTQ8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Bidirectional sugar transporter SWEET</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_016029</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Mediates both low-affinity uptake and efflux of sugar across the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the SWEET sugar transporter family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006990">
        <property type="protein sequence ID" value="KAI7746376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CTQ8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051119">
        <property type="term" value="F:sugar transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.290:FF:000001">
        <property type="entry name" value="Bidirectional sugar transporter SWEET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.290:FF:000002">
        <property type="entry name" value="Bidirectional sugar transporter SWEET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.290">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047664">
        <property type="entry name" value="SWEET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004316">
        <property type="entry name" value="SWEET_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10791:SF111">
        <property type="entry name" value="BIDIRECTIONAL SUGAR TRANSPORTER SWEET2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10791">
        <property type="entry name" value="RAG1-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03083">
        <property type="entry name" value="MtN3_slv"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="76"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="107"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="133"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="169"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="194"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU910715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7746376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="232" mass="25765" checksum="FDD9D1C68196DE84" modified="2024-05-29" version="1">MVSPQTFEALKQAAGIAGNIFAFGLFVSPIPTFRRIIRNQSTEQFSGIPYVYALLNCLICAWYGCPFISYDNLLVTTVNSVGAVFQLTYIIIFITHADKNKKFKMSGLLLAVFGLFAAIAIGSLLITDLEVRHLLIGFLSCATLISMFASPLSVMNVVIQTRSVEFMPFYLSLSTFLMSTSFLLYGVFNFDPFIYVPNGIGTILGIAQLALYFYYNNQSKQESREPLIESVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CUH7</accession>
    <name>A0AAD5CUH7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Solanesyl diphosphate synthase 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_018287</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the FPP/GGPP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006920">
        <property type="protein sequence ID" value="KAI7746675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CUH7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004659">
        <property type="term" value="F:prenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010236">
        <property type="term" value="P:plastoquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00685">
        <property type="entry name" value="Trans_IPPS_HT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000092">
        <property type="entry name" value="Polyprenyl_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033749">
        <property type="entry name" value="Polyprenyl_synt_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02749">
        <property type="entry name" value="prenyl_cyano"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001:SF69">
        <property type="entry name" value="ALL TRANS-POLYPRENYL-DIPHOSPHATE SYNTHASE PDSS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001">
        <property type="entry name" value="GERANYLGERANYL PYROPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00348">
        <property type="entry name" value="polyprenyl_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00723">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00444">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7746675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="415" mass="45949" checksum="8CE0DB169D06EE03" modified="2024-05-29" version="1">MMMMLATCHNLEFGSCGCYFNSLSNRLKFYKRKVIRSVNKGYCGGVGSRRLIYRRSDGNVAKCGVFSPEARETLFNGDASGLPSRLSEKKESTTSLFEAVSDDLLTLNKNLQSIVGAENPVLMSAAEQIFSAGGKRMRPALVFLVSRATLELTGLEELTREHRRLAEIIEMIHTASLIHDDVLDESDMRRGKETVHQLYGTRVAVLAGDFMFAQSSWYLANLENLEVIKLISQVIKDFASGEIKQASSLFDCDVKLDEYLIKSYYKTASLIAASTKGAAIFSAVDNDISEQMYQYGKNLGLSFQVVDDILDFTQSAEQLGKPAGSDLAKGNLTAPVIFALEKQSKLREIIETEFCETGSLNEAIELVMNSDGIERARVLAKQKAEVAIQCLQCLPPSSFRLALEGMVNFNLERID</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CVM1</accession>
    <name>A0AAD5CVM1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Stomatal closure-related actin-binding protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_003133</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006472">
        <property type="protein sequence ID" value="KAI7748754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CVM1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010119">
        <property type="term" value="P:regulation of stomatal movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.440">
        <property type="entry name" value="ATP synthase delta/epsilon subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039640">
        <property type="entry name" value="SCAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032012">
        <property type="entry name" value="SCAB-ABD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032015">
        <property type="entry name" value="SCAB-Ig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041144">
        <property type="entry name" value="SCAB-PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032009">
        <property type="entry name" value="SCAB_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31172">
        <property type="entry name" value="STOMATAL CLOSURE-RELATED ACTIN-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31172:SF7">
        <property type="entry name" value="STOMATAL CLOSURE-RELATED ACTIN-BINDING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16711">
        <property type="entry name" value="SCAB-ABD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16709">
        <property type="entry name" value="SCAB-Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17684">
        <property type="entry name" value="SCAB-PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16712">
        <property type="entry name" value="SCAB_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Stomatal closure-related actin-binding protein actin-binding" evidence="2">
        <location>
            <begin position="55"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="Stomatal closure-related actin-binding protein coiled-coil" evidence="3">
        <location>
            <begin position="101"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="Stomatal closure-related actin-binding protein Ig" evidence="1">
        <location>
            <begin position="282"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="Stomatal closure-related actin-binding protein PH" evidence="4">
        <location>
            <begin position="382"/>
            <end position="490"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF17684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7748754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="497" mass="55662" checksum="3ACA84B8B992ACC1" modified="2024-05-29" version="1">MTKVSPEIFDDMQMQAVLSVSADVSFKSNKFPKYKLGPDNQILDEAAVDDTGPPLKDIVEQETAALSDQHKRLSVRDLASKFDKNLSAAAKLSDEAKLKEVASLEGHVLLKKLRDALEYLRRRLVGRNKEDVEKAISMVEALAVKLTQNEGELIQEKSEVKKLANFLKQASEDAKKLVNQERSFACAEIESAKAVVRRIGEALDEQEKMSLSSGKQEMEEMVEEIQQARRIRLLHHPCKVMDMEHELRALRLQIQEKSAFSVKLQQELLLKKLVEENKLSLYALEGSVSLGSILRLQPCSEEAMELSKCSIQWYRLSSQCSRRELISGANKSVYAPEPYDVGRILQVDIISNGQKSTVTTACPVQPAVGLESYVETLSRKGNSEFSVVISQMNGRNYTTHSAHLLRIGKMRMKLSKGWITKARDAYNSTSMQLCGFRGGGNLAAKSIFWQSRKGQSFVLVFESERERNAAVVLARRYALDCNVVLAGPDDQASLYLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CYY1</accession>
    <name>A0AAD5CYY1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Dof zinc finger protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_026919</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Transcription factor that binds specifically to a 5'-AA[AG]G-3' consensus core sequence.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006056">
        <property type="protein sequence ID" value="KAI7750938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CYY1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045174">
        <property type="entry name" value="Dof"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003851">
        <property type="entry name" value="Znf_Dof"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31992:SF159">
        <property type="entry name" value="DOF ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31992">
        <property type="entry name" value="DOF ZINC FINGER PROTEIN DOF1.4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02701">
        <property type="entry name" value="Zn_ribbon_Dof"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01361">
        <property type="entry name" value="ZF_DOF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50884">
        <property type="entry name" value="ZF_DOF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 8 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3 9" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 8 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Dof-type" evidence="10">
        <location>
            <begin position="58"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU369094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7750938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="305" mass="33368" checksum="1670DFAB04CB49AA" modified="2024-05-29" version="1">MNITSLEVCMDSSDWFQGTVPEELKIDSMSPSGLSDSMLTCSQPLMDRRLRPPQDHHLKCPRCDSTHTKFCYYNNYSLTQPRYFCKTCRRYWTKGGTLRNIPVGGGCRKPKKVVSTNKKTTIHNHKNSHLVDINPNPNYNHDTNNNNELVMSSRDLQLSYPEAKMKYSGLNMYGNANSGDVRSFMFENMSSGYYGGVNVMGQSYRGGICSPFGGMTLDSINGSLVERGLIGYTREGNGVNDDGIGGFVDVKPNVLALEWQDDDQNGASATSSGAGGGDSGYLTGFGSSWGGLVHGYGPPTTNPLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CZM8</accession>
    <name>A0AAD5CZM8_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_012227</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006162">
        <property type="protein sequence ID" value="KAI7750352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CZM8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009658">
        <property type="term" value="P:chloroplast organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901259">
        <property type="term" value="P:chloroplast rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017126">
        <property type="term" value="P:nucleologenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.40:FF:000016">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044673">
        <property type="entry name" value="DCL-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33415">
        <property type="entry name" value="PROTEIN EMBRYO DEFECTIVE 514"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33415:SF12">
        <property type="entry name" value="PROTEIN EMBRYO DEFECTIVE 514"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11523">
        <property type="entry name" value="DUF3223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="181"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="181"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="194"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7750352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="205" mass="23034" checksum="3B2F8EAEB6B3E215" modified="2024-05-29" version="1">MAEEQTDVVQAPADDQSMDLETVGKRGREEDVTTENGNEAKKPKVDDKSVEEQSVEKQITITKSGPVSVGYKEFNESVEMFDYFFKLLHYWPPNLNLNKYEHTMLLDLLKKGHLEAEKKMGTGVGAFQVRYHPQWKSRCFFLVRVDGSADDFSFRKCVDHILPLPENMVVKSDVNKALGGGHTGCGRGGGRGNWRGRGRGGKPRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D054</accession>
    <name>A0AAD5D054_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_021887</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006324">
        <property type="protein sequence ID" value="KAI7749585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D054"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050220">
        <property type="term" value="F:prostaglandin-E synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12782">
        <property type="entry name" value="MICROSOMAL PROSTAGLANDIN E SYNTHASE-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12782:SF5">
        <property type="entry name" value="PROSTAGLANDIN E SYNTHASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7749585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="90" mass="10590" checksum="E0460C228BD0025A" modified="2024-05-29" version="1">MFYHRIFTGTLLRLWNPSTISQAMVRHKYGNFGFTEKYTVKYAGAAAMYFVSKKLKKKYNITDERKALYEAAETWVDALDGRDFLGQFSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D2X7</accession>
    <name>A0AAD5D2X7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Branched-chain-amino-acid aminotransferase</fullName>
            <ecNumber evidence="9">2.6.1.42</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_027362</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-isoleucine + 2-oxoglutarate = (S)-3-methyl-2-oxopentanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24801"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:35146"/>
            <dbReference type="ChEBI" id="CHEBI:58045"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:18321"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:17865"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-valine + 2-oxoglutarate = 3-methyl-2-oxobutanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24813"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57762"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.42" evidence="9"/>
    <dbReference type="EMBL" id="JAMZMK010006047">
        <property type="protein sequence ID" value="KAI7750950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D2X7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004084">
        <property type="term" value="F:branched-chain-amino-acid transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009082">
        <property type="term" value="P:branched-chain amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01557">
        <property type="entry name" value="BCAT_beta_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.10.10:FF:000003">
        <property type="entry name" value="Branched-chain-amino-acid aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.10:FF:000003">
        <property type="entry name" value="Branched-chain-amino-acid aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.10.10">
        <property type="entry name" value="D-amino Acid Aminotransferase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001544">
        <property type="entry name" value="Aminotrans_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018300">
        <property type="entry name" value="Aminotrans_IV_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036038">
        <property type="entry name" value="Aminotransferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005786">
        <property type="entry name" value="B_amino_transII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043132">
        <property type="entry name" value="BCAT-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043131">
        <property type="entry name" value="BCAT-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033939">
        <property type="entry name" value="BCAT_family"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01123">
        <property type="entry name" value="ilvE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009897">
        <property type="entry name" value="PRK13357.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42825">
        <property type="entry name" value="AMINO ACID AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42825:SF29">
        <property type="entry name" value="BRANCHED-CHAIN-AMINO-ACID AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01063">
        <property type="entry name" value="Aminotran_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006468">
        <property type="entry name" value="BCAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56752">
        <property type="entry name" value="D-aminoacid aminotransferase-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00770">
        <property type="entry name" value="AA_TRANSFER_CLASS_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="3 9" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="9" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="5 8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="6">
        <location>
            <position position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR006468-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7750950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="379" mass="41990" checksum="A82AB2D0A687201E" modified="2024-05-29" version="1">MIRQSPFLRRLVQISSSKVGARCLTAQAASALRESEPMVRYEEYADIDWNNLGFGLTRTDYMYKAKCTLDNNFEQGQVTDYGNVELSPAAGVLNYGQGIFEGTKAFRGEDGRIFLFRPDQNAIRMQIGAERMCMQSPSIQQFVDAVKQTTIANKRWIPPPGKGSLYLRPLLIGTGPTLGLSPAPEYTFLVYASPVGNYFKEGTAPLNLYVNNEFHRATRGGAGGVKSITNYAPVIKAMLKAKEQGYSDVLYLDSVHKKYIEEVSSCNIFLVKGNVISTPATVGTILEGITRKSIIDIARDLGYKVEERLVAIDELMEADEVFTTGTAVSVASVGSVTYNDQRLEYKTGDESVSQKLYKTLVGIQLGKIEDKYNWVVEIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D5N7</accession>
    <name>A0AAD5D5N7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_031978</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005258">
        <property type="protein sequence ID" value="KAI7753824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D5N7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041928405" description="Transmembrane protein" evidence="2">
        <location>
            <begin position="24"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7753824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="81" mass="9248" checksum="6D04CEEE2E0DEDF5" modified="2024-05-29" version="1">MDLKNCLTTVCILFLLLAVPCFSKERPNSEVYDIDYRGPETHSHRPPPNKTGSGFPHSNLKKHPRFKRQATRNATKKGRKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D8X8</accession>
    <name>A0AAD5D8X8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Nop domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_021389</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NOP5/NOP56 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000329">
        <property type="protein sequence ID" value="KAI7756638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D8X8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031428">
        <property type="term" value="C:box C/D methylation guide snoRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.246.90:FF:000004">
        <property type="entry name" value="Nucleolar protein 58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.4070">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.90">
        <property type="entry name" value="Nop domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045056">
        <property type="entry name" value="Nop56/Nop58"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012974">
        <property type="entry name" value="NOP58/56_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042239">
        <property type="entry name" value="Nop_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002687">
        <property type="entry name" value="Nop_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036070">
        <property type="entry name" value="Nop_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012976">
        <property type="entry name" value="NOSIC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10894">
        <property type="entry name" value="NUCLEOLAR PROTEIN 5 NUCLEOLAR PROTEIN NOP5 NOP58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10894:SF1">
        <property type="entry name" value="NUCLEOLAR PROTEIN 58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01798">
        <property type="entry name" value="Nop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08156">
        <property type="entry name" value="NOP5NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00931">
        <property type="entry name" value="NOSIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89124">
        <property type="entry name" value="Nop domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51358">
        <property type="entry name" value="NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Nop" evidence="6">
        <location>
            <begin position="262"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="432"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="432"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="483"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="519"/>
            <end position="532"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7756638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="532" mass="59052" checksum="BE2F7396EB8C838B" modified="2024-05-29" version="1">MLVLFETPAGFALFKVLDEGKLSKADDLWKEFSSADTARQFVKLKAFSKFENTSEALSAATLLLESKPSKGLRKFLRAHCDGESLAVADSKLGNVIKEKLQIDCVHTQAVMELMRGVRSQLTELITGLGAQDLAPMSLGLSHSLSRYKLKFSPDKLNTYAMRVREWYGWHFPEFAKIVQDNILYAKAVMLMGYRTNAAKLDFSEILSEEIEAELKEAAVVSMGTEVSDLDLVNIKDLCDQVLSLSKYRAQLYDYLKSRMNTIAPNLTAIVGELVGARLIAHGGSLLNLAKQPGSTVQILGAEKALFRALKTKHATPKYGLIYHASLIGQAAPKHKGKISRSLAAKTALAIRCDALGDNQDNSMGVENRLKLEARLRNLEGRELGRSAGSTKGKPKIEAYNKDFKKGDGAMITPAKTYNAAADSVLGRIEAEAEKDIEMAPSDEGKKDKKKKKKKTVADEDEEEEAEEGKKKDKKKKKHAAVEETVEVQNEEENVEKKKKKKRKHAEADDEDEDEAEKSSKKKKDKKKKKTEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D9C7</accession>
    <name>A0AAD5D9C7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Excitatory amino acid transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_007099</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010001297">
        <property type="protein sequence ID" value="KAI7755299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D9C7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36052">
        <property type="entry name" value="EXCITATORY AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36052:SF1">
        <property type="entry name" value="EXCITATORY AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7755299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="78" mass="8763" checksum="946DD6CE36A10DF5" modified="2024-05-29" version="1">MPASATMIGALLGLGTQMYSNALRKLPYMRHPWEHVLGMGLGAVFANQLVKWDAKLQHDLDDMLDKAKAANERRYFGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DBP7</accession>
    <name>A0AAD5DBP7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Reticulon-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_032574</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Endoplasmic reticulum membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000020">
        <property type="protein sequence ID" value="KAI7758176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DBP7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009617">
        <property type="term" value="P:response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003388">
        <property type="entry name" value="Reticulon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045064">
        <property type="entry name" value="Reticulon-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10994">
        <property type="entry name" value="RETICULON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10994:SF164">
        <property type="entry name" value="RETICULON-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02453">
        <property type="entry name" value="Reticulon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50845">
        <property type="entry name" value="RETICULON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="51"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="75"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="Reticulon" evidence="7">
        <location>
            <begin position="42"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7758176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="139" mass="16632" checksum="B101D5CE2014CDB8" modified="2024-05-29" version="1" fragment="single">MAIPYSDLDNDNKRYENHDNKSYQNMANFKRYFHSFIGNGQVSDVLLWRNTRLSASILFIVTAVWYYFEVYEHNFVSLVCYAAMVAMFFICVTYTMAKYTQWDLPDFEEITIPESKFKWLYKKTNELLLKFYHTASGED</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DCY6</accession>
    <name>A0AAD5DCY6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_014111</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010001017">
        <property type="protein sequence ID" value="KAI7755570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DCY6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10567">
        <property type="entry name" value="SWIB-MDM2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.200">
        <property type="entry name" value="Plus-3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.245.10">
        <property type="entry name" value="SWIB/MDM2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045894">
        <property type="entry name" value="At5g08430-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003169">
        <property type="entry name" value="GYF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035445">
        <property type="entry name" value="GYF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058668">
        <property type="entry name" value="NERD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004343">
        <property type="entry name" value="Plus-3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036128">
        <property type="entry name" value="Plus3-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036885">
        <property type="entry name" value="SWIB_MDM2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003121">
        <property type="entry name" value="SWIB_MDM2_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46851:SF11">
        <property type="entry name" value="GYF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46851">
        <property type="entry name" value="OS01G0884500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02213">
        <property type="entry name" value="GYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25980">
        <property type="entry name" value="NERD_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03126">
        <property type="entry name" value="Plus-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02201">
        <property type="entry name" value="SWIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00444">
        <property type="entry name" value="GYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00719">
        <property type="entry name" value="Plus3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55277">
        <property type="entry name" value="GYF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159042">
        <property type="entry name" value="Plus3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47592">
        <property type="entry name" value="SWIB/MDM2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50829">
        <property type="entry name" value="GYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51360">
        <property type="entry name" value="PLUS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51925">
        <property type="entry name" value="SWIB_MDM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DM2" evidence="4">
        <location>
            <begin position="32"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="Plus3" evidence="3">
        <location>
            <begin position="167"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="GYF" evidence="2">
        <location>
            <begin position="531"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="353"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7755570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="584" mass="66170" checksum="BFFB1C30E4B4D39B" modified="2024-05-29" version="1">MGDRNGTFYWVGDADDLTLSPVKQRRKSKSKKLEFVGWGSRSLIEFLQSIGKDTGKQLSQYDVTAIINEYVHTYNLFNPMKKKRVVCDERLRSLFSKNSVPRNKIYELLNSHFAANNDSSEDDDLYNSEEVEDVNVPYKKPKVSSSDKIAADQKKKASQARLSCFAAIIPENIKLVYLKRSLVQELVKHPESFEGKLLGSYIRTKSDPLDFSQKNSHQLHPVTAVKNVPGINGVHEVLLQVPNVVKDIPIRLLSDDGFSTEEVEDLCQRVKDGLLKRPTVVEFEQKARILHEDITKHWLPRELAHLQNLIDRANEKGWPLTLFEYLERKKLLQTPSEQSKLLTEVPTVTADTLESGADIEQPNASPVSIHGSDAAEDKLVDDDAVDEGKAEPIKNSGSEEQLASFQWVEAVQMPNKRSFSQTIDFSKTKNSGSAEHLAGFQWLESVQMPKTQSLGQTVEFSKIKSAGSGENLTSFQWVEAVQMPKTRSVGQTVDLSKMSNVIDLCDDEEEAHDAQANKETHEQEDEHEFSCRKWFYRDPQGETQGPFSKFELKSWRDAGYFLPDFKVWKEGQTPEYAILLTAIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DES0</accession>
    <name>A0AAD5DES0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_032577</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000020">
        <property type="protein sequence ID" value="KAI7758179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DES0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7758179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="110" mass="12438" checksum="56069351B4F1BF29" modified="2024-05-29" version="1">MDSLHLRSLRSSLAQIWIGVESICAFKIPFSNLLLVIKSKNTVNRTLPKAQVQQLNNNVLVLLSFVKQTLCLAANQQPFGCPWLQHTLILFLIIIDICAKMCLRFKGEQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G325</accession>
    <name>A0AAD5G325_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_024776</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the PP2C family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010011844">
        <property type="protein sequence ID" value="KAI7725846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G325"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000222">
        <property type="entry name" value="PP2C_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832:SF810">
        <property type="entry name" value="PROTEIN-SERINE_THREONINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01032">
        <property type="entry name" value="PPM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0464">Manganese</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="10">
        <location>
            <begin position="23"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7725846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="277" mass="30323" checksum="B1FFFBEA66B3BF06" modified="2024-05-29" version="1">MGIYLSKPKTEKISEDGENEKLRYGLSSMQGWRTSMEDAHAAYPDLDCSTSFFGVYDGHGGQAVSKFCAKYLHQQVIKHEAYASGDIGTAVQKSFLRLDEMMCGQRGWRELAILGDKKDQFSGMMEGLIWSPRSVETKGQTDDWPVEEGPHSNYEGPTCGSTACVAIIRNNQLIVANSGDSRCVISRKGQAYNLSKDHKPDLESEKERISKAGGFIHCGRVNGTLNLTRAIGDMELKKDKSLPAEKQILTANPDINTGLHVKPTAGGLCTGANKNCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G438</accession>
    <name>A0AAD5G438_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Amino acid transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_002805</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011282">
        <property type="protein sequence ID" value="KAI7727960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G438"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48017">
        <property type="entry name" value="OS05G0424000 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="34"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="58"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="184"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="233"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="275"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="316"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="8">
        <location>
            <begin position="28"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7727960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="378" mass="41505" checksum="010E922C70541CA2" modified="2024-05-29" version="1" fragment="single">MQKPSSSSFENMEVMNGDFDDDGRIRRTGTLMTTSAHVMAVVIGSGVLSLAWCLAQLGWIIGVPLLGAFAVITWYTCILLTDCYRSPDPVTGTRNYNYMQAVKVNLGGFKYKLCGISQYSTQIGTSIGYAITSAISMAAVKRSNCFHMYGHNNGCHTLNNTYVLIFGAIEIVLSQIPNFHELSLVSVVATIMSFAYAGIGVALSVAKIAGGTSSKTSLMGIAVGPNMTRMEKMWNAFSAIGDIAFAYSFSFVLLEIQDTLKQSPPENKVMKRSCTIGIGASSLLYMICAILGYLAFGNDSPGNYLTGFGFYEPFWLVNIGNICVVIHLLGAFQAIIQPFFAVVENWSRKKWPGCKFIEREYIIGKYSINLFRLTWRTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G4W4</accession>
    <name>A0AAD5G4W4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_003437</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011339">
        <property type="protein sequence ID" value="KAI7727646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G4W4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01814">
        <property type="entry name" value="Ubl_MUBs_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040015">
        <property type="entry name" value="UBL3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039540">
        <property type="entry name" value="UBL3-like_ubiquitin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13169:SF0">
        <property type="entry name" value="UBIQUITIN-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13169">
        <property type="entry name" value="UBIQUITIN-LIKE PROTEIN 3 HCG-1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13881">
        <property type="entry name" value="Rad60-SLD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="1">
        <location>
            <begin position="7"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7727646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="129" mass="14417" checksum="AD9F1B12B24D6816" modified="2024-05-29" version="1">MATDELIEVKFRLADGTDIGPSKYSSITTVGSLKEKVISQWPKDKENGPKTINDMKLINAGKILENNKTLAESRSPVSEIGGIITMLVVVRPPMAEKNSGRLPEKEQLFLHNPLKFIRRSQTTFQDFSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G6Y8</accession>
    <name>A0AAD5G6Y8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Centromere/kinetochore protein zw10-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">M8C21_015751</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">AA19_3_7</strain>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Centromere</location>
            <location evidence="2">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ZW10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010948">
        <property type="protein sequence ID" value="KAI7729658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G6Y8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990423">
        <property type="term" value="C:RZZ complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007094">
        <property type="term" value="P:mitotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046362">
        <property type="entry name" value="Zw10/DSL1_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048343">
        <property type="entry name" value="ZW10_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055148">
        <property type="entry name" value="ZW10_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048344">
        <property type="entry name" value="Zw10_middle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009361">
        <property type="entry name" value="Zw10_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12205">
        <property type="entry name" value="CENTROMERE/KINETOCHORE PROTEIN ZW10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12205:SF0">
        <property type="entry name" value="CENTROMERE_KINETOCHORE PROTEIN ZW10 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20666">
        <property type="entry name" value="ZW10_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22766">
        <property type="entry name" value="ZW10_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20665">
        <property type="entry name" value="Zw10_middle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06248">
        <property type="entry name" value="Zw10_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0132">Cell division</keyword>
    <keyword evidence="10" id="KW-0137">Centromere</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="7" id="KW-0498">Mitosis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Centromere/kinetochore protein zw10 N-terminal" evidence="11">
        <location>
            <begin position="39"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="Centromere/kinetochore protein zw10 middle" evidence="12">
        <location>
            <begin position="270"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="Centromere/kinetochore protein zw10 C-terminal" evidence="13">
        <location>
            <begin position="364"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="domain" description="ZW10 C-terminal helical" evidence="14">
        <location>
            <begin position="515"/>
            <end position="671"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF06248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF20665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF20666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF22766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAI7729658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="676" mass="76103" checksum="F1130C1AF264DD54" modified="2024-05-29" version="1">MDVLFNSINVRDLLSSHDLDDSSPLSTPDLRLLIDRLNIRSLDIKSKLHTYILSHHHDFSDLFINSSNAVVLSDQLFTHVSDLNKLVSKPIDVEIKQTVDQIRLKHVELKEKKDALGLVVVVLELSKELGLVENDLVVWKVVEAAERLMELKLALRVTEECENEGEGEPVVFGLLRKKWTDSFEEIQKVLVKFIEKAVVGILDYGLAKVADLMTRHVITPAVGSISHSFSVEEQRLDSGDITEAVLRITPLSGTQVENMDAENIYSGISIVPDDASKLSDFQTIRQLTSEFETSLKEVMFISSEKDERLTNFTDNVEVHFATRKKVGILATARDLLLHSDFKLPKEYSRKSEDMKNGGNVDLLFSSERCAISEAASQLLELVHQTLKDVCLSSPTVALEFCHAARDALLLYEAVIPVKLERQLDGINQVAVLIHNDCLFLSQEILGLKFQYQSDFPTSMKGAVFIDLGIRYKLMAEEILQRQIKLVKHNLMEAIDGADGFQNTHQPKRYESAKFSIDQVAFIIEKVHIIWEPLLIPLTYKRSMCNVLEAVCSRMTKDILQLDDIAADETLQIQRLIHMLLENLSSLLASLVGINQTTKPQEALSGSIDDLIPSLPKLRVLADMLDMPLKSITASWESGELANCGFTASEVKDFIRAIFTDSPLRKECLWRIENIKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G730</accession>
    <name>A0AAD5G730_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Mitochondrial substrate carrier family protein ucpB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_030841</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010578">
        <property type="protein sequence ID" value="KAI7730989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G730"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050391">
        <property type="entry name" value="Mito_Metabolite_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45618">
        <property type="entry name" value="MITOCHONDRIAL DICARBOXYLATE CARRIER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="8">
        <location>
            <begin position="18"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="8">
        <location>
            <begin position="110"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7730989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="210" mass="22286" checksum="974D62B4D10A3F32" modified="2024-05-29" version="1" fragment="single">MGVKNNQSWSASPSNVASHFGTSGVAVATATGITHPLDVLKVRLQMQLVGQRGPLTGMGQTFVQVVKKEGPQALYNGLTPALTRSVVYGGLRLGLYEPSKNLCELAFESTNIFMKIASGVVSGAFATLLTNPMEVVKVRLQMNPNVHIGPIGELQKIAAKEGVTALWKGVGPAMARAGALTASQMATYDEFKRVLVTEGPRGLYKGFPNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G7W9</accession>
    <name>A0AAD5G7W9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_009179</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010387">
        <property type="protein sequence ID" value="KAI7731784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G7W9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766:SF70">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN RGA4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766">
        <property type="entry name" value="PLANT BROAD-SPECTRUM MILDEW RESISTANCE PROTEIN RPW8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0611">Plant defense</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7731784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="550" mass="62340" checksum="C9821BB3DA85FD10" modified="2024-05-29" version="1">MLGWKRWSTKDAVFPCLRELYIESCPQLVEVSAESLPLLRVLKISKSGHHVLRSLVQAASSITMLEILDISGLTYEVWSGVMEHIGAVEEVSIEECNEIRYLWESEEEASKVLVNIKKLVVYNCSNLVSLGKKEEEDKDNFGSSLLSSLRALDVCECNKMERCCCPNSIESLHIKWCRSLTHVSFPTAATGGGGGGQKLKSLEIYGCSKLMEKINNISMGVLERIDIGVWINLKSLMQLGNFIYLTQLYLYKCPSLESFPDIPLPVLTCLEIEDCERMESFSAHQMSNNLTSLKELVIRDCPSIDASSHVGVWPPNLCSLEIGRLKNPMTEWGPQSFSSSLVDLKLYYDADVKNFRQLSHLLLPSSLTKLRIYNFEKLESLSMGLQHLTSLQHLTIKYCPKMKHLPKHLTSLQHLTIWGCPKIKHLPKQLLPSLLSLEIVYCPKLRKNPLISHIPDIIMNELKEASAVATFSWVSKKELGILAQHLRTEAVKKRNSSKRNGRTKLDWAVAIATFNWVSKEEVGMLAQQTQWQNKIGTPLKRNLEHNNEYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-04-02" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G8F3</accession>
    <name>A0AAD5G8F3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cytochrome P450 94B3-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_010051</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010260">
        <property type="protein sequence ID" value="KAI7732312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11064">
        <property type="entry name" value="CYP86A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24296">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7732312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="417" mass="47709" checksum="D5ACA2564DB2CFA4" modified="2024-05-29" version="1">MSSTQTIVLTRIGGRRNQMIVTANPANVEHILKTKFDNYPKGKPFTDLLGNFLGMGIFNVDGELWSTQRKLASHEFSAKSLREFVVSVLEEEVTKRLFPLLENAVQNGDVLDMQDVLRRLAFDTICKISLGWDPCCLDYSRCVPPLAPAFDAAAEASAMRGRALATWVWKVKRFFNVGSERKLKEAVGVVHRAVNEIIRERREQMDGSDNHRDLLSRFISAGHNEDLVRDMVISFLMAGRDTTSAAMTWLFWLLQRHPAIEKEVLKDVTIKQNDKLNFDDLKQMDYLKACLCESMRLYPPVVWDSKHAASDDVLPDGTRVYKGNRVMYFPYGMGRMEALWGKDCLEFRPDRWFVEPGVLKIESPYKFPVFQGGPRVCLGKEMXXXXXXXXXXXXXXXXXXXXSVRMNRFLCHCLRLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G8F7</accession>
    <name>A0AAD5G8F7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Actin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_011349</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the actin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010541">
        <property type="protein sequence ID" value="KAI7731113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G8F7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd10224">
        <property type="entry name" value="ASKHA_NBD_actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000131">
        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000291">
        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000001">
        <property type="entry name" value="Actin, muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020902">
        <property type="entry name" value="Actin/actin-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004001">
        <property type="entry name" value="Actin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00190">
        <property type="entry name" value="ACTIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00406">
        <property type="entry name" value="ACTINS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01132">
        <property type="entry name" value="ACTINS_ACT_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7731113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="356" mass="39266" checksum="8694B87A2013B2D2" modified="2024-05-29" version="1" fragment="single">MADSEDIQPLVCDNGTGMVKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDAYVGDEAQSKRGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNAPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDSLMKILTERGYSFTTSAEREIVRDMKEKLAYIALDYEQELETSKTSSAVEKSYELPDGQVITIGAERFRCPEVLFQPSMIGMESAGIHETTYNSIMKCDVDIRKDLYGNIVLSGGTTMFPGIADRMSKEITALAPSSMKIKVVAPPERKYSVWIGGSILASLSTFQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G8J7</accession>
    <name>A0AAD5G8J7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_028873</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010499">
        <property type="protein sequence ID" value="KAI7731233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G8J7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043067">
        <property type="term" value="P:regulation of programmed cell death"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42647">
        <property type="entry name" value="SBP (S-RIBONUCLEASE BINDING PROTEIN) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42647:SF45">
        <property type="entry name" value="TRANSCRIPTION FACTOR C2H2 FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7731233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="371" mass="42490" checksum="704496278B194FE0" modified="2024-05-29" version="1">MSLISMAVDASHRDMIYQGDASRLDYPFHTAYTNKTEEIHPMYGSGMTDLFSAKAESGDILAYTFPCSRKRLRESSFCIPKVPIVHPNQYQFGSLEYLSDDIASQIYQQQLEIDSFVAQHTEKVKTSIKEIRRRNTIKLTAAYEEMANCLKTKEEEIAKIAQLNRSLEEKVKSITIENQIWRELAQTNEATANTLRNNLQQVLIEIQLQQGHQNCLESNCNVDAESHSGSNFDEVNEKDARGLGTINNEFQYFNTSGTNNIRWCRICGKEESCVLRAHGSRLLMSEGTRAYLVSLRKKERGTVSTLNEWVHYIICTLRLDGCTKSKEMAFADVEEREMGGEDVEERVAFEREMGGEDVEERVAFEIVLRWM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2024-10-02" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G9B5</accession>
    <name>A0AAD5G9B5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_004474</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010021">
        <property type="protein sequence ID" value="KAI7733347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7733347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="38" mass="4542" checksum="A087837FD63487B5" modified="2024-05-29" version="1">MYXXXXQFGSLMVWNLKPAPNHHSTYIAHFFRNLQLCM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G9M9</accession>
    <name>A0AAD5G9M9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tRNA-intron lyase</fullName>
            <ecNumber evidence="3">4.6.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_020690</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>pretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.</text>
            <dbReference type="EC" id="4.6.1.16"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VAMP-associated protein (VAP) (TC 9.B.17) family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the tRNA-intron endonuclease family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.16" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010009928">
        <property type="protein sequence ID" value="KAI7733707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G9M9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000213">
        <property type="term" value="F:tRNA-intron lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090158">
        <property type="term" value="P:endoplasmic reticulum membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061817">
        <property type="term" value="P:endoplasmic reticulum-plasma membrane tethering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006388">
        <property type="term" value="P:tRNA splicing, via endonucleolytic cleavage and ligation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22363">
        <property type="entry name" value="tRNA-intron_lyase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000813">
        <property type="entry name" value="Vesicle-associated protein 1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000535">
        <property type="entry name" value="MSP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008962">
        <property type="entry name" value="PapD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036167">
        <property type="entry name" value="tRNA_intron_Endo_cat-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006677">
        <property type="entry name" value="tRNA_intron_Endonuc_cat-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016763">
        <property type="entry name" value="VAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10809:SF156">
        <property type="entry name" value="MAJOR SPERM PROTEIN (MSP)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10809">
        <property type="entry name" value="VESICLE-ASSOCIATED MEMBRANE PROTEIN-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00635">
        <property type="entry name" value="Motile_Sperm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01974">
        <property type="entry name" value="tRNA_int_endo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49354">
        <property type="entry name" value="PapD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53032">
        <property type="entry name" value="tRNA-intron endonuclease catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50202">
        <property type="entry name" value="MSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MSP" evidence="5">
        <location>
            <begin position="8"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00034031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7733707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="311" mass="34757" checksum="E8A748A20C5BF8B1" modified="2024-05-29" version="1">MKKMKPQDLEVLPKQLNFIYEPKKQSSCSVQIVNRSDHHVAFEIKTANSENYCVQPNTGVIDPNSTCEFLVTMQAQKVAPLDMTCRDTFLLQSTVVPAGTKQEDVASNTFAKEHGRVVEEKMLDVIIIPPTSKLVQNNEDRVLKGDLLQKNSFHAKILMGPRWKGKGSEVKALANPISKSIAGGDHGIKTDNELWEYMSSRKASFPNFFAAYSHLRVKNWVVRSGCQYGVDFVAYRHHPSLVHSEYGVLVLSQGNGNDRLRVWSDIQCTLRLCGSVAKTLLILHVNKKDENMVIVWKSIQSQDGVQSAAGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G9T6</accession>
    <name>A0AAD5G9T6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Inositol polyphosphate-related phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_025400</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the inositol polyphosphate 5-phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009686">
        <property type="protein sequence ID" value="KAI7734605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G9T6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004445">
        <property type="term" value="F:inositol-polyphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034485">
        <property type="term" value="F:phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004439">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045849">
        <property type="entry name" value="IP5P_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000300">
        <property type="entry name" value="IPPc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45666:SF34">
        <property type="entry name" value="TYPE IV INOSITOL POLYPHOSPHATE 5-PHOSPHATASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45666">
        <property type="entry name" value="TYPE IV INOSITOL POLYPHOSPHATE 5-PHOSPHATASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22669">
        <property type="entry name" value="Exo_endo_phos2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Inositol polyphosphate-related phosphatase" evidence="3">
        <location>
            <begin position="4"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7734605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="99" mass="11813" checksum="D41080B587F8D683" modified="2024-05-29" version="1" fragment="single">FGSQLRIEQRRGRVFQGWSEGKIYFPPTYKYLTNSDRYTGDSLHHKEKRRTPAWCDRILWYGAGLHQLSYVRGESRSRQQTRVCNGYARSFICWFIISS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GAQ0</accession>
    <name>A0AAD5GAQ0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RHOMBOID-like protein</fullName>
            <ecNumber evidence="9">3.4.21.105</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_007248</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Serine protease involved in intramembrane proteolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>Cleaves type-1 transmembrane domains using a catalytic dyad composed of serine and histidine that are contributed by different transmembrane domains.</text>
            <dbReference type="EC" id="3.4.21.105"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Membrane</location>
            <topology evidence="2 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the peptidase S54 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.105" evidence="9"/>
    <dbReference type="EMBL" id="JAMZMK010010049">
        <property type="protein sequence ID" value="KAI7733273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GAQ0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1540.10:FF:000019">
        <property type="entry name" value="RHOMBOID-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1540.10">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002610">
        <property type="entry name" value="Peptidase_S54_rhomboid-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022764">
        <property type="entry name" value="Peptidase_S54_rhomboid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22936:SF79">
        <property type="entry name" value="RHOMBOID-LIKE PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22936">
        <property type="entry name" value="RHOMBOID-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01694">
        <property type="entry name" value="Rhomboid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0720">Serine protease</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="61"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="143"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="176"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="200"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="230"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="253"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="301"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S54 rhomboid" evidence="11">
        <location>
            <begin position="134"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="30"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="39"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7733273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="411" mass="45642" checksum="D40774C4BDA722EC" modified="2024-05-29" version="1" fragment="single">MEETHRPEIRLKVNSRRGFGGGNIIHPVEVESSPPTPFPATTTTQSQTPSPFREVKHFKKWVPWLIPTFVIADTVLFIITMYVNDCPKNSVSCIATFLKRLSFQPFKENPLLGPSSNTLHKMGALDVSKVVDDHQGWRLITCIWLHGGLFHLLANMLSLLVIGIRLEQEFGFIRIGLLYVISGFGGSLLSALFLQSNISVGASGAVFGLLGAMLSELITNWTIYANKMSALLTLVIIIVINLAVGILPHVDNFAHLGGFFSGFLLGFVFLMRPQFGWVNQRYTPAYPRTGAKPKYKKYQQVLWVLSLVLVVAGMIAGLVSLLRGVNLNDHCSWCHYMSCVPTSRWSCNTDPVSCLSEQTGNQYTLTCSNSGKNGTYLLDNPNQSKIRGLCIPEHFEGLITVYSSCLYLCYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GBE7</accession>
    <name>A0AAD5GBE7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_017909</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009853">
        <property type="protein sequence ID" value="KAI7734008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GBE7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01837">
        <property type="entry name" value="SGNH_plant_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050592">
        <property type="entry name" value="GDSL_lipolytic_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035669">
        <property type="entry name" value="SGNH_plant_lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45642">
        <property type="entry name" value="GDSL ESTERASE/LIPASE EXL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45642:SF101">
        <property type="entry name" value="GDSL-LIKE LIPASE_ACYLHYDROLASE SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7734008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="343" mass="37683" checksum="7777FF905621706A" modified="2024-05-29" version="1">MAVLVVHAKAIDVAQLRRIAAKNNVSCIYVFGDSSVDPGNNNNLMTDLKGNFLPYGKDFYNGQPTGRFTNGRLATDLIADALGHTKAIPAYLDPNLTNAKLANGVSFASGGSGYDDLTAQLSNVISLSKQLEYFRECKARLGRVVGKKRSREIVTNAVFLLSMGTNDFLQNYYVEPTRSKQFTIDKYQDFLISAMTKYIKAIHAEGAMRMAVVGMEPFGCSPLVRALRGTVACDDEYNKAALSFNSKIKSLVATLGPSLPIKIFYIDIYGLIQGTIQNPKQYGFVETSKGCCGSGLELGQSCKGLPTCANRREYIYWDAVHFTEEMYKIISEEALKSLMKSLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GBK5</accession>
    <name>A0AAD5GBK5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_008661</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009392">
        <property type="protein sequence ID" value="KAI7736032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GBK5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7736032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="41" mass="4869" checksum="06AE9981D8DC0969" modified="2024-05-29" version="1">MMSFLRVLKGGHASGSPSTSQRHQRHRFPVFQFTHLYLIRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GC23</accession>
    <name>A0AAD5GC23_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_002730</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009630">
        <property type="protein sequence ID" value="KAI7734876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GC23"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034005">
        <property type="term" value="F:germacrene-A synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016102">
        <property type="term" value="P:diterpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046246">
        <property type="term" value="P:terpene biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00684">
        <property type="entry name" value="Terpene_cyclase_plant_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.600.10:FF:000007">
        <property type="entry name" value="Isoprene synthase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.130:FF:000001">
        <property type="entry name" value="Isoprene synthase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.130">
        <property type="entry name" value="Terpene synthase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034741">
        <property type="entry name" value="Terpene_cyclase-like_1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044814">
        <property type="entry name" value="Terpene_cyclase_plant_C1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001906">
        <property type="entry name" value="Terpene_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036965">
        <property type="entry name" value="Terpene_synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050148">
        <property type="entry name" value="Terpene_synthase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005630">
        <property type="entry name" value="Terpene_synthase_metal-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31225">
        <property type="entry name" value="OS04G0344100 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31225:SF9">
        <property type="entry name" value="TERPENE SYNTHASE 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01397">
        <property type="entry name" value="Terpene_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03936">
        <property type="entry name" value="Terpene_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01019">
        <property type="entry name" value="Terpene_Cyclase_Like_1_C_Termi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Terpene synthase N-terminal" evidence="4">
        <location>
            <begin position="36"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="Terpene synthase metal-binding" evidence="5">
        <location>
            <begin position="260"/>
            <end position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7734876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="554" mass="64443" checksum="176445BAB348324B" modified="2024-05-29" version="1">MASILLALQSTPIALLNQNAVEPLTRRSGNYAPTLWSFDFIQSLSSEYAGEAYKARADTLMEALKMMITNVGNPLSSLELVDDLQRLGISYHFEYEISNLLEKIYCNNYKSHDEWRKMGLNLKALGFRLLRQRGYRVPQEIFQNFKDEVEDTTGVLNLYEASYHSFEDESILDDAREFATKYLKENQYNMNESMSILVSHALEVPQHWSVSRVEAKWFIPVYENRSDMNPLLLELAKLDYNIVQAGYIEDVKHASRWWKNTSWDKKLTFARDRLVECFLWTVGFTYQPKFSPGRRNVARVNAMITTIDDVYDVYGTVEELEQFTNIITRWDINAIEELPDYMKICFLGFYNTINEIAYNTMIESGVLILPYLKKAWAELCKSYLVEAKWYYSGHTPTLQEYLDNAWVSISSPLVFMHIRFSSSISSTEEILQWFEESKNIIYLASFICRLADDLGTSSDEMARGDVPKAMQCYMNESGATEDEARRYIKNLMLKTWKKLNKERESARSQHLREFIEYAMDGVRMTQFMYGKGDGHGNPNMTESHLQSLLFNPIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GC95</accession>
    <name>A0AAD5GC95_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_009041</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009134">
        <property type="protein sequence ID" value="KAI7737050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GC95"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034098">
        <property type="term" value="C:VCP-NPL4-UFD1 AAA ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031593">
        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097352">
        <property type="term" value="P:autophagosome maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051228">
        <property type="term" value="P:mitotic spindle disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030970">
        <property type="term" value="P:retrograde protein transport, ER to cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.20.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015415">
        <property type="entry name" value="Spast_Vps4_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF200">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 48 HOMOLOG E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09336">
        <property type="entry name" value="Vps4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA ATPase AAA+ lid" evidence="5">
        <location>
            <begin position="11"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="Spastin/Vps4 C-terminal" evidence="4">
        <location>
            <begin position="70"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="123"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="47"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="123"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF09336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF17862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7737050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="144" mass="15894" checksum="A3F3B2FDCAC10147" modified="2024-05-29" version="1" fragment="single">AAMRKSPVSKDVDLRALARYTQGFSGADITEICQRACKYAIRENIEKDIERERKRSENPDSMDEDEDDVAEIKAAHFEESMKYARRSVSDADIRKYQAFAQTLQQSRGFGSEFRFSETSGGAAAAASDPFAASGGGADDDDLYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GCB3</accession>
    <name>A0AAD5GCB3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_033599</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009557">
        <property type="protein sequence ID" value="KAI7735131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GCB3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.455.10">
        <property type="entry name" value="Ribosomal protein S7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000235">
        <property type="entry name" value="Ribosomal_uS7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036823">
        <property type="entry name" value="Ribosomal_uS7_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11205">
        <property type="entry name" value="RIBOSOMAL PROTEIN S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47973">
        <property type="entry name" value="Ribosomal protein S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7735131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="177" mass="20224" checksum="0E35F7A4B75C47FF" modified="2024-05-29" version="1">MQVLYSDGDEEVLDLHTEKWQMVDEISPDQVSDLSAEDYITATASKNPIYMPHTAGSGPREDATRIGSTGVVRRHTIDISPLRRVNQAIYLLTTQERISENRVSNQPFEAILELARLKDDDLYRSIDIFLMALIYAKPFDMEGEKNIEVATPQIVEKRKISGQVVVYRDWLLELNRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GCB9</accession>
    <name>A0AAD5GCB9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">UDP-glycosyltransferases domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_021660</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009611">
        <property type="protein sequence ID" value="KAI7734938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GCB9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080043">
        <property type="term" value="F:quercetin 3-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080044">
        <property type="term" value="F:quercetin 7-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926">
        <property type="entry name" value="GLUCOSYL/GLUCURONOSYL TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926:SF1392">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7734938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="298" mass="33964" checksum="BB8E0F14216ECB92" modified="2024-05-29" version="1" fragment="single">TNIRTRERDVKTITITDGLPDDHPCSGDRILDVFEGMRTMTGPVFKEMMECECVLLRLLYQMECFLLWLMWRKRLGYRAHGLILNTFQGLDESILVHMQTLCPNIYCIGPIHTLYNSRVTNEPKVTTQTPQSIVSNSLWEENPFLWVQRPGSIIGEYDVNQVPKHLADETKQRGFITTWVPQEAVLAHTAIGVFLTHSGWNSTIESIMEGVPMICWPQNVDQQVNSRFVSEVWKIGIDIKDTCDRAIVEKAVRDVIDARRSEFIESTRMLGELGGQSVADDGASVMDLYRLIKTISSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GDK1</accession>
    <name>A0AAD5GDK1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_008435</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008822">
        <property type="protein sequence ID" value="KAI7738372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001680">
        <property type="term" value="P:tRNA 3'-terminal CCA addition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05398">
        <property type="entry name" value="NT_ClassII-CCAase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3090.10">
        <property type="entry name" value="cca-adding enzyme, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002646">
        <property type="entry name" value="PolA_pol_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032828">
        <property type="entry name" value="PolyA_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052191">
        <property type="entry name" value="tRNA_ntf/polyA_polymerase_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43051">
        <property type="entry name" value="POLYNUCLEOTIDE ADENYLYLTRANSFERASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43051:SF1">
        <property type="entry name" value="POLYNUCLEOTIDE ADENYLYLTRANSFERASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01743">
        <property type="entry name" value="PolyA_pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12627">
        <property type="entry name" value="PolyA_pol_RNAbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81891">
        <property type="entry name" value="Poly A polymerase C-terminal region-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Poly A polymerase head" evidence="6">
        <location>
            <begin position="87"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA nucleotidyltransferase/poly(A) polymerase RNA and SrmB- binding" evidence="7">
        <location>
            <begin position="235"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="551"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="663"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="590"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="663"/>
            <end position="672"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF12627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7738372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="688" mass="77752" checksum="0F2F55B6A795F2BD" modified="2024-05-29" version="1">MAATNIKRDSFIARLKPRLSQLFYHKLVKHGNKPRPRSDKDSVSEPGKIDISKWRKMDSRALGITRVMVPSSPYTLLKILRSRGFEAYLVGGCVRDLLLNRPPKDFDVITTADLHXXXXSPKFPICRVHIKGSVIEVSSFKTLAKDSEEKEKFLVSQMPRGCDKFDLNLWKNSMRRDFTVNSLFFDPLNYKIYDYNNGMKDLLELKLRTLVPAHVSFTEDCARILRGLRIAARLGLSFSKDIEAAIHKHASSILSLSASRIMMEMDYMLSFGAAESSLRLLHRFHILEILLPFQAAYISRQPTGPEQHTMMLMKLFSHMDELVSCDQPSACCLWIGLLAFHQALLSRPQHPFVVFTFGCVLYHQSWEDGLKFARKYCQTLISFDPETSDPYEFIFDDEVAKKVTILAKRVIDSIDVLVDTDTLHKTMSKFPGFPCPGMVFVSKNAAHSAAQLFHVLAHKVETYNKGRSSFEMNHQLLGKGDALETRFALGKIIMNTMGCRVNLDNHPGPPEYIQSTLSDSWLQKPNAKSSTSQKQIVEDIEILVTSGLKIEGSTMEKPEEISASRSPTQKSSNSKKQKLNTKSSNSKNAFSDKEIKDIAATDHSIGKPEEMCASDLPKQERMTTPEPCLHNGNNNRNEKLPSQLEAQNSKEKVNVVAEVKVDESTTKEEGVSRKQKRKQVLPLSSLFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GE14</accession>
    <name>A0AAD5GE14_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">mitogen-activated protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_033570</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 16">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.24" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010009209">
        <property type="protein sequence ID" value="KAI7736708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GE14"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004707">
        <property type="term" value="F:MAP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07859">
        <property type="entry name" value="STKc_TDY_MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000017">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000046">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000578">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003527">
        <property type="entry name" value="MAP_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01351">
        <property type="entry name" value="MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="21"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="415"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="471"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="425"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7736708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="497" mass="57127" checksum="9732CF3AFB96AFD7" modified="2024-05-29" version="1">MDPHKRLEREFFTEYGEAGRYQVHEVVGKGSYGVVGSATDTHTGEKVAIKKINDVFEHVSDATRILREVKLLRLLRHPDIVEIRHIMLPPSRREFRDIYVVFELMESDLHQVIRANDDLTPEHHQFFLYQLLRGLKYIHSANVYHRDLKPKNILANADCKLKICDFGLARVSFNDAPTAVFWTDYVATRWYRAPELCGSFFSKYTPAIDIWSIGCIFAEMLMGKPLFPGKNVVHQLDLMTDLLGTPPPETIARIRNEKARRYLNNMRKKQPVPFTQKFPNVDPLALKLLERLLAFDPKDRPSAEESLADPYFNGLSNVDREPATQAIPKLEFEFERRKLGKDDVRELIYREILEYHPQMLQEYLRGGEQTSFLYPSGVDRFKRQFAHLEEGLGKGGNGKTTPLLRHHASLPRQVTEFPSVWERVPAPKEDGDSQENDSEKETSDSVEDPENSNETVKGYYTARSLLKSASISASKCIGGNGTRPTEEELDENKLYEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GE26</accession>
    <name>A0AAD5GE26_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribosome maturation factor RimM</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_031813</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008695">
        <property type="protein sequence ID" value="KAI7738927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GE26"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003977">
        <property type="term" value="F:UDP-N-acetylglucosamine diphosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006048">
        <property type="term" value="P:UDP-N-acetylglucosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.240:FF:000002">
        <property type="entry name" value="Ribosome maturation factor rimM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.240">
        <property type="entry name" value="PRC-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.60">
        <property type="entry name" value="RimM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00014">
        <property type="entry name" value="Ribosome_mat_RimM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011033">
        <property type="entry name" value="PRC_barrel-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056792">
        <property type="entry name" value="PRC_RimM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011961">
        <property type="entry name" value="RimM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002676">
        <property type="entry name" value="RimM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036976">
        <property type="entry name" value="RimM_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039741">
        <property type="entry name" value="UDP-sugar_pyrophosphorylase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02273">
        <property type="entry name" value="16S_RimM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11952:SF10">
        <property type="entry name" value="16S RRNA PROCESSING PROTEIN RIMM FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11952">
        <property type="entry name" value="UDP- GLUCOSE PYROPHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24986">
        <property type="entry name" value="PRC_RimM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01782">
        <property type="entry name" value="RimM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50346">
        <property type="entry name" value="PRC-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RimM N-terminal" evidence="1">
        <location>
            <begin position="78"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosome maturation factor RimM PRC barrel" evidence="2">
        <location>
            <begin position="179"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7738927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="639" mass="71711" checksum="9ABD689767B22EC0" modified="2024-05-29" version="1">MEGCSLLCGSTSLPTSLPLLLHYPVKFLTPFKPSKCFSRRTLTCIKPHPIPISSSLHPTTATQETVEASEIESQYVEIGYISSVHGLQGEVRVKPSTDFPELRFSEPGKRWLKQQLLGREIIQEVELLEGRGHHGQKSWIVKFSNVDKVEQAQQLVGSTILVLDEDRPELEEGDFYARDLAGMRVMLKETGEPVGTVVNVFDVGGNDLLQVKLDSSLEKIDKNGNLKSEAPLVWIPFVEAIVPHVDLNAREMIITPPKGLLELNVRSDERSKKERRQLEWKERKKFQKQLIAAKKKLCEMEQKHVFDGLRHGEKAQRNLLADQILDVNSQLLQVALQTIETPSERWQFSKFLTAFDTEKTKDVFKVSKGCLDSEGVKPTISKIAERRSALVSSGKVANILVIEGDMLKTSDSEGTDSLIQRLVEMENCHTTPLILICPDNTIETFQNLMSNNDYFGFDPEKVWILEEEKLPVVNSSPGENKKHKILMKSPWEILQTPVGSGGVISLLSSHNILESLAAMGVEYIEISSVDQRHIGGENLLGLVDSSEAHVGIKTFNGIDGVDNDFYLVFSINFLTQLTKRASKLTFHALLRSNQHVEMADKEWVDITPSSHNSYEFRSSIYSCLEGCSLDKVCVMEIVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GEN1</accession>
    <name>A0AAD5GEN1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_026747</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008612">
        <property type="protein sequence ID" value="KAI7739527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GEN1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="126"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7739527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="192" mass="21999" checksum="0E0AA31BE36D57E7" modified="2024-05-29" version="1">MKKRVYDYEGFSSLYKQKCLMEEPVATKEGYPSRILRDVCWDYPLRYAVSLLKLDNGATIEARFKDFCKVKLSMDENDLEEAMRLFIQSKHLFWTNAFTLGSGTVMRLIEPGQELRTCKDHDFGRRSESAHLRGDSKGSARGGDGSGRKDNIESQLASNAQLAHQGYFVNSQVWISRFFPSPSLLRLHFSIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GEU5</accession>
    <name>A0AAD5GEU5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">30S ribosomal protein S8, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_001020</name>
        <name evidence="5" type="ORF">M8C21_002157</name>
        <name evidence="6" type="ORF">M8C21_002158</name>
        <name evidence="7" type="ORF">M8C21_011730</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uS8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011535">
        <property type="protein sequence ID" value="KAI7726781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAMZMK010011535">
        <property type="protein sequence ID" value="KAI7726782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAMZMK010008714">
        <property type="protein sequence ID" value="KAI7738849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAMZMK010008619">
        <property type="protein sequence ID" value="KAI7739478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GEU5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.30:FF:000001">
        <property type="entry name" value="40S ribosomal protein S15a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.10:FF:000002">
        <property type="entry name" value="40S ribosomal protein S15a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01302_A">
        <property type="entry name" value="Ribosomal_uS8_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000630">
        <property type="entry name" value="Ribosomal_uS8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047863">
        <property type="entry name" value="Ribosomal_uS8_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035987">
        <property type="entry name" value="Ribosomal_uS8_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003115">
        <property type="entry name" value="PRK04034.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11758">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S15A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00410">
        <property type="entry name" value="Ribosomal_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56047">
        <property type="entry name" value="Ribosomal protein S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00053">
        <property type="entry name" value="RIBOSOMAL_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7726781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7726782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7738849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7739478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="130" mass="14848" checksum="08F0C9858EFA4A13" modified="2024-05-29" version="1">MVRVSVLNDALKSMYNAEKRGKRQVMIRPSSKVIIKFLMVMQKHGYIGEFEYVDDHRSGKIVVELNGRLNKCGVISPRFDVGVKEIEPWTARLLPSRQFGYIVLTTSAGIMDHEEARRKNVGGKVLGFFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GHY6</accession>
    <name>A0AAD5GHY6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_028790</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008324">
        <property type="protein sequence ID" value="KAI7740906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GHY6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7740906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="70" mass="7374" checksum="F2BB99C429CBF836" modified="2024-05-29" version="1" fragment="single">FPPLFSPSSFFNTHNYPVYGSVGQRRINSGTNVSSEINGGGFEAVVVSDMNSGSGGVLCSTVPTTDFLLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GJF8</accession>
    <name>A0AAD5GJF8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_003547</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="JAMZMK010007738">
        <property type="protein sequence ID" value="KAI7743364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GJF8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050684">
        <property type="term" value="P:regulation of mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000245">
        <property type="term" value="P:spliceosomal complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000339">
        <property type="entry name" value="Serine/threonine-protein kinase SRPK-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051334">
        <property type="entry name" value="SRPK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47634:SF21">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47634">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="1"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="143"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="144"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="177"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="204"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7743364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="458" mass="51321" checksum="AD0B9873F31B9D0D" modified="2024-05-29" version="1">MDEITILKQIAEGDPDDKKCVVKLLDNFKHSGPNGQHVCMVFEYLGDNLLTLIKYSDYRGVPLHKVKEICFHILGGLDYLHRQLSIIHTDLKPENILLLSMIDPERDRTKAGAPIIPSSSKDNNVTNTSVSKEVKILNSDLTKNQKKKIKKKAKKAAQNVSGKEDNEEAEADNETTSNGAESFPSQKSNGGSLEEHATSSVVKSESRNLSEARDSRQGNQLRGRGSRSTRQRLLAEVDVKCKLVDFGNACWTYKQFTGDIQTRQYRCPEVLLGSKYSTPADLWSFACICFELATGDVLFDPHSGDNYDRDEDHLALMMELLGTMPRKIALGGRYSREFFNRHGDLRHIRRLRFWPLHKVLKEKYEFSEQDATDLADFLVPLLDFAPEKRPTAAELLTHRWLTGGPPYDLTPAVNSTTKASDIKSCEKTREIDEQEAMEAGVGNIAIGGVSKPLPTHAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GK87</accession>
    <name>A0AAD5GK87_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_019000</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007063">
        <property type="protein sequence ID" value="KAI7746005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GK87"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009909">
        <property type="term" value="P:regulation of flower development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010402">
        <property type="entry name" value="CCT_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045281">
        <property type="entry name" value="CONSTANS-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31319:SF110">
        <property type="entry name" value="CCT MOTIF FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31319">
        <property type="entry name" value="ZINC FINGER PROTEIN CONSTANS-LIKE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06203">
        <property type="entry name" value="CCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51017">
        <property type="entry name" value="CCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCT" evidence="5">
        <location>
            <begin position="296"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="335"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="336"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="363"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7746005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="372" mass="42108" checksum="8802B8375B81729E" modified="2024-05-29" version="1">MLHDVVHPPNQPPMEQVSSPLSAQILEFCESDLFQETIQNSDVASASNCYEEQSSYVNGISFPPDMTKYTTPTTTTTTTATTTDGGNDNYTPLFEEKNIENDISAPQEYTNIHEYPFSNQDQFDLSLLQNQLQFAMDGPIMPYPHPNDQATDVLSMMGVCESECMSMPPSKCMRLNNNNNPSSPNHCFLDHPYFPSRNSNSMLPIESCGIFNGSLSFANEIQAHELDFLGDNGGFFFPDPLPRPYNSNELQALSNESQHLVNNGNGSCNHPLAPPEITSLESESFRVANKLTSEERKEKIHRYMKKRNERNFSKKIKYACRKTLADSRPRVRGRFAKNDEFGEHHRNSCNTHEEDTDEDVKSMHVRTKGQQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GLE3</accession>
    <name>A0AAD5GLE3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">S-acyltransferase</fullName>
            <ecNumber evidence="8">2.3.1.225</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Palmitoyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_018090</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="8">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="8"/>
    <dbReference type="EMBL" id="JAMZMK010007655">
        <property type="protein sequence ID" value="KAI7743848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GLE3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12246">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="51"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="149"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="9">
        <location>
            <begin position="103"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7743848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="279" mass="31168" checksum="E6168B87E0FC0E51" modified="2024-05-29" version="1">MGMGMGKVKGWKKMRWSGHVIVVSLAIGYIYLSSVLVFIKAWFGLGSSPGIINALLFTALALLCISNYALAMFTDPGSVPPSFAPDIEDPLHPIHEIKRKGGDLRYCKKCCLYKPPRSHHCRICKRCVLRMDHHCVWMNNCVGHANYKLFFVFVAYALLACLYSLILLLGSLTVDTQQSEGPYRSVYVHHFRAVANSTEHGFRCFSGLAYLPNLTKQNHNRDQGGNVYSHPYDLGVYENLITVLGPNVLCWVCPVSSYIGSGLHFRTAYDRHATATTTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GNU8</accession>
    <name>A0AAD5GNU8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">M8C21_010526</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">AA19_3_7</strain>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the leguminous lectin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006316">
        <property type="protein sequence ID" value="KAI7749650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GNU8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002229">
        <property type="term" value="P:defense response to oomycetes"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06899">
        <property type="entry name" value="lectin_legume_LecRK_Arcelin_ConA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000168">
        <property type="entry name" value="L-type lectin-domain containing receptor kinase IX.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000240">
        <property type="entry name" value="Lectin-domain containing receptor kinase A4.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050528">
        <property type="entry name" value="L-type_Lectin-RKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000985">
        <property type="entry name" value="Lectin_LegA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001220">
        <property type="entry name" value="Legume_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27007">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00139">
        <property type="entry name" value="Lectin_legB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00308">
        <property type="entry name" value="LECTIN_LEGUME_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0430">Lectin</keyword>
    <keyword evidence="13 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 18" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042106904" description="Protein kinase domain-containing protein" evidence="18">
        <location>
            <begin position="19"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="267"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="334"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="364"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAI7749650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="645" mass="71900" checksum="2FE9558B4CDEE361" modified="2024-05-29" version="1">MAMIFLVCSFSLLHFSESVDFKIDRFDVDATNILYHGDATPSVGMIQFTKDNYLTRVGQAVYHDTVPIWDSKSGKLTDFNTHFTFMIDTQGRSDYGHGLTFFLAPVTFEIPPNSAGGFLGLFNTTYIDSPRNQMIVIEFDSFVNSDWDPPFEHVGINKNSISSVAYTAWNASLHSGDTADVWVTYNAPTQLLNMRWSYGDGNGTRANENTSLSYQVDLREVLPERVMVGFSAATSANVERNFLKSWEFNSSLNLVDQDENNSKAWKMAVGIGVPLGVLALVVMAACVLFWRKQRKLVEEPVEEVALTSMNEDLERGAGPKRFSYNELVLATNNFSDDRKLGKGGFGCVYKGYLTRENMDVAVKKISQGSKQGKKEYITEVKVISSLRHRNLVRLIGWCHDQTNFLLVYEFMPNGSLDSHLFGNKSPLEWSVRYKIATGLASALLYLHEEWEQCVVHRDIKTSNIMLDSGFNVKVGDFGLARLMDHELGPQTTGLAGTLGYMAPEYVTTGKASKDSDVYSFGVVVIEIASGRKAIDNVDPDSDLGLVNWVWGLLGKGELLFGVDQRLNDAFDAKQVECLMMVGLWCAHPNRSLRPSIRQALQVLKFEGALPNLRMKMPMPMYCANPDVPQGSGGGSITYTDIDLVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-06-18" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GRU4</accession>
    <name>A0AAD5GRU4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">ABC transporter B family member 20</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">M8C21_029213</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">AA19_3_7</strain>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005635">
        <property type="protein sequence ID" value="KAI7752680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd18577">
        <property type="entry name" value="ABC_6TM_Pgp_ABCB1_D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18578">
        <property type="entry name" value="ABC_6TM_Pgp_ABCB1_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000028">
        <property type="entry name" value="ABC transporter B family member 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000240">
        <property type="entry name" value="ABC transporter B family member 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001683">
        <property type="entry name" value="ABC transporter B family member 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000021">
        <property type="entry name" value="ABC transporter B family member 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24222">
        <property type="entry name" value="ABC TRANSPORTER B FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24222:SF52">
        <property type="entry name" value="ABC TRANSPORTER B FAMILY MEMBER 20-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="664"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="722"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="762"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="839"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="864"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="938"/>
            <end position="965"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1458"/>
            <end position="1489"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1509"/>
            <end position="1531"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1585"/>
            <end position="1605"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1611"/>
            <end position="1634"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="14">
        <location>
            <begin position="24"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="16">
        <location>
            <begin position="750"/>
            <end position="1006"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="15">
        <location>
            <begin position="1039"/>
            <end position="1274"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="16">
        <location>
            <begin position="1462"/>
            <end position="1664"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="15">
        <location>
            <begin position="1685"/>
            <end position="1922"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="607"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1298"/>
            <end position="1438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1926"/>
            <end position="1954"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1319"/>
            <end position="1328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1337"/>
            <end position="1386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1389"/>
            <end position="1409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1416"/>
            <end position="1427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1429"/>
            <end position="1438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="1931"/>
            <end position="1941"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="17">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAI7752680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="19">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1954" mass="216530" checksum="15F3489CDBF3C829" modified="2024-05-29" version="1" fragment="single">MEWETQRRRKKKQQRQPNKDEETTTFYISNLPSYCSLGMLWKEFRVCGKMSNAYLPRRRDKAGRFFAFIRFDGVKDVEEMTKALTRVRAGEWNIHANVAKFSKVEDNSPHGFRRDDVSLQQGFEKYEPVVNDKGKQPICDDVNQFPPIQGCPKMNIVSVPNKTYQIQPVLGRCSLIGEVIDVTRLCRMEDFLTALGLKDVSVRLSEREGGGVEELVFTVWIGGVTTWLKISGVPVQLWDKEVIDRIAKSFGTVVQGSRASERDGNFSFENVAVLVKDGKRISSGVELRWRNVRINVWVEEMEETWCPDFVLAKAYPNRKIELNVENADYVFEKSMEEGPSNNQASNLAEDAIEECEFVIENEPGSPVIAPVVCTSPVTEKSKDHTEGERSLHGEMVDINDVLGNGDTCMLDEDVAQVPSDLQQIGCFNLEGGSSRSNIISNNNKSNGVPGENNDGVTPIPPSMGKMSRNLRSPPSLGSIQAPPTRACYGDISPGSKRNEIKSIENLPVESGVEMARSHDAQVISGTSRDRIYILVSKFLNPNCPIQDHYEMKQIKNSRTILDNNQVLKRIRTTMCTTNKPEKCSNLGFYKSKGLFGWSPPHVQPLTPVSEVSEPPESPSPYMDPTNDALPVEAEDEMEETEEIEPPPAAVPFSKLFVCADRFDWVLMVVGSVAAAAHGTALVIYLHYFAKIVQLLGRGGDEADELFDRFKEYGLERNQYRSLAHVLLMLPLGQDIVTSHWLISAGDSCNVELQKNNNDTYGLALILVYIAGGVFAAGWIEVSCWILTGERQTAVIRSKYVQVLLNQDMSFFDTYGNNGDIVSQVLSDVLLIQSALSEKVGNYIHNMATFFSGLAIGFINCWQIALITLATGPFIVAAGGISNIFLHRLAENIQDAYAEAASVAEQAISYVRTLYAFTNETLAKYSYANSLQATLRYGILISLVQGLGLGFTYGLAICSCALQLYVGRFLVTHRKAHGGEIVTALFSIILSGLGLNQAATNFYSFEQGRIAAYRLFEMISRSSSAVDHNGNTLATVQGNIEFRNVYFSYLSRPEIPILSGFYLTVPAKKTVALVGRNGSGKSSIIPLMERFYDPTLGEVLLDGENIKNLKLEWLRSQIGLVTQEPALLSLSIRDNIAYGRDSTSLQIEDAAKIAHAHTFITSLEKGYDTQVGRAGISLTEEQKIRLSVARAVLSNPSILLLDEVTGGLDFEAEKSVQEALDLLMLGRSTIIIARRLSLIKNADFIAVMEDGQLMEIGTHDELIASDGLYAELLRCEEAAKLPRRMPARTYDETTALQIEKDSSESQNFQEPSSPKFAKSPSLQRTSNVHASRPQDSAYESHESPRNPSPTEKENGLSLDGTDKEPSIKRQDSFEKRLPELPKLDIHAIRRQTSNGSNPESPISPLLTSDPQSERSHSQTFSRPNSQLTDKPIRVQPLKEKQSKKEPSVWRLIELSLAEWLYAVLGSTGAAIFGSFNPLLAYVIALIVTAYYRNEIDRHINIRDDVDKWCLIIACMGFVTVIANFLQHFYFGIMGEKMTERVRRMMFSAMLRNEVGWFDDEENSADTLSMRLANDATFVRAAFSNRLSILIQDSSAVIVAILIGMLLHWRLALVALATLPVLTLSAIAQKLWLAGFSKGIQEMHRKASLVLEDAVRNIYTVVAFCAVPKIEPDDAAGLKPPNVYGSIELKNVDFSYPTRPEILVLSNFSLKVSGGQTVAVVGVSGSGKSTIISLIERFYDPVAGQVMLDGRDLKQFNLRWLRNHLGLVQQEPIIFSTTIKENIIYARHNASDAEIKEAARIANAHHFISSLPHGYDTHVGMRGVDLTPGQKQRIAIARVVLKNAPILLLDEASSSIESESSRVVQEALDTLVMGNKTTILIAHRAAMMRHVDNIVVLNGGRIVEDGTHDSLMAKNVISPVVLKELIDGDKPTNGGGDGGSGGGYRHRVTRLKKDQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GTJ1</accession>
    <name>A0AAD5GTJ1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">M8C21_031096</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">AA19_3_7</strain>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010002238">
        <property type="protein sequence ID" value="KAI7754947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GTJ1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090404">
        <property type="term" value="C:pollen tube tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010183">
        <property type="term" value="P:pollen tube guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000266">
        <property type="entry name" value="probable serine/threonine-protein kinase RLCKVII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000032">
        <property type="entry name" value="Serine/threonine-protein kinase PBS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47985">
        <property type="entry name" value="OS07G0668900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47985:SF41">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE PBL5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 13 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 13 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 14" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="130"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="415"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="46"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="95"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="430"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="11">
        <location>
            <position position="257"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="262"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000615-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000615-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAI7754947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="439" mass="48686" checksum="D564BFE874A7C73E" modified="2024-05-29" version="1">MGCFPCAGGDSSAKKTSKNKRIEHTHNDQSLSKPSDTIKVEQCNQVKKENAPNVKKENAPNVKKENAPIVKKENAPNVKKENAPNVVQTSVSSEVVEKSNEKNKPTPEGGPVNKATTFTYAQLVNATDNFKGAYFLGEGGFGKVYKGKLEDSDQIVAIKQLDPNGLQGIREFVVEVLTLGLADHPNLVKLIGYCAEGEQRLLVYEYMPLGSLEDHLHGFRANSKRLDWNTRMKIAAGAARGLEYLHDKMNPPVIYRDLKASNILLGEDYHAKLSDFGLAKVGPLGDKTHVSTRVMGTYGYCAPDYAMTGQLTFKSDIYSFGVVLLELITGRKAIDNTRSAAEQNLVAWARPLFKDRRKFWQMADPVLEGEYPVRGLYQALAIAAMCVQEQPNMRPLIADVVTALNYLASQKYDPSVNPVQTRRSSHRQQRSIDEKKPDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GTZ6</accession>
    <name>A0AAD5GTZ6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_031691</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010001074">
        <property type="protein sequence ID" value="KAI7755517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GTZ6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7755517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="177" mass="20003" checksum="BAE49EE77BCB58BC" modified="2024-05-29" version="1" fragment="single">HHRTTYSNGTWTSTIPTTTPAAKRIKRDTPIIINNARYRKSTGVKYNRTTGFQVQQALQLYATERKRNCVVNEIIRSSFQELNMFGIGRRTAAAISLLQVFKRCCEWVGTCSEIKKLWTAASNFAPWPSKSGSSLELFDIDPHMSYKDGLQQAIQATFDVSCDLVAMPYPCATEKQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-06-18" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GUD7</accession>
    <name>A0AAD5GUD7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_023581</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000677">
        <property type="protein sequence ID" value="KAI7755952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03233">
        <property type="entry name" value="ABCG_PDR_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03232">
        <property type="entry name" value="ABCG_PDR_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002615">
        <property type="entry name" value="ABC transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000179">
        <property type="entry name" value="ABC transporter G family member 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003489">
        <property type="entry name" value="ABC transporter G family member 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029481">
        <property type="entry name" value="ABC_trans_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034001">
        <property type="entry name" value="ABCG_PDR_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034003">
        <property type="entry name" value="ABCG_PDR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013581">
        <property type="entry name" value="PDR_assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48040">
        <property type="entry name" value="PLEIOTROPIC DRUG RESISTANCE PROTEIN 1-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48040:SF44">
        <property type="entry name" value="SULFATE-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19055">
        <property type="entry name" value="ABC2_membrane_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14510">
        <property type="entry name" value="ABC_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08370">
        <property type="entry name" value="PDR_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="559"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="596"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="679"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="705"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="785"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1240"/>
            <end position="1258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1278"/>
            <end position="1298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1318"/>
            <end position="1344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1356"/>
            <end position="1377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1383"/>
            <end position="1403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1415"/>
            <end position="1434"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1464"/>
            <end position="1487"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="191"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="857"/>
            <end position="1146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7755952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="1495" mass="169606" checksum="8E432AAC397E57D5" modified="2024-05-29" version="1">MASALTGDDLMRTMSRNMSRNMSRNMSRNMSRNMSRGGSLASGSTRGWGAASIREVFTTPGTDVFQKSGREDDEDELMWAAIERLPTYDRLRKGFLTNILDDGKVVREEVDVSNLGPEERHNLMKNMQKAIDDDNERLLERLRDRIDRVGIDIPRIEIRYQNLSIQGDVFVGSRALPTLFNSTINSIEGVLGFLRLFPSKKRVVNILHEVSGIVAPSRMTLLLGPPGSGKTTLLKALAGKLENDLRVMGKVTYCGHEMNEFVPQRTCAYISQHDLHHGELTVRETLDFSGRCAGVGTRYEMLAELSRREKAEGIKPDPELDAFMKAIAVSGQKSSLVTEYVLRLLGLDICSDTMVGDDMRRGISGGQKKRVTTGEMLVGPAKVFLMDEISTGLDSSTTFQIIKYMRQMVHIMDITMIISLLQPAPETFELFDDIVLLSEGQVVYQGPRTNVLEFFEHVGFKCPERKGVADFLQEVTSKKDQEQYWFNKSIAYRYVSVDDFVHHFQNYHIGRKIAGILHVPYDKSKAHPAALVTNKYGISNKELFKACLSREWLLMKRNAFVYIFKTTQITIMALFTFTVFFRTTMKYGDLDDGGKYYGALFFSLINVMFNGMAELALTIFRLPVFYKQRDSLFYPAWAFALPVWLLRLPLSLLESGIWIVLTYYTVGFAPSASCFFRQLLAYLGIHQMALGLFRFIAALGRTQVVANTLGTFTLLLVFVLGGFIIAKDDLQPWMKWAYYLSPMSYGQNAIVLVEFLDKRWSAPIIGSTVGKELLKARGMFTEGHWYWTCVLALFGFSLIFNLCFMLALTYLTPMVDSKSIVDDNDEKNKKLAKIESESEPNGLNKKGMVLPFKPLSLVFDHVNYYVDMPAEMKSQGVEEDRLQLLRDVSGAFRPGVLTALVGVSGAGKTTLMDVLAGRKTGGYIEGSISISGYPKNQETFARVSGYCEQNDIHSPHVTVYESLLYSAWLRLASDVDEATRKVCTKMLFSRTTKTQSYKFKEILLREIGTEIKHINSTFMFVEEVMELVELGPIRNALVGLPGVDGLSTEQRKRVTIAVELVANPSIIFMDEPTSGLDARAAAIVMRAVRNTVDTGRTVVCTIHQPSIDIFEAFDELFLMKRGGQAIYAGPLGRYSHLLVEYFESIDGVSKIKEGYNPATWMLEVTSTAVESQLGVDFAEIYANSDLYRRNEELIKELSTPPPDSQDLHFATQYSQPSFEQFKACFWKQHKSYWRNPQYNVVRFFMTTVIGLIFGIIFWNKGTKISKQQDLSNLMGAMYAAVLFLGGTNTSAVQSVVAIERTVFYREKAAGMYSALPYAFAQALVETIYVGIQTFLYSLLLYYMIGFEWQLGKFLWFYYYVFMCFVYFTLYGMMLVALTPSYQIAAIVMSFFLSFWNLFSGFLIPRGQIPIWWRWYYWGSPVAWTIYGLITSQLGDMSTRVFVPKHGLMEVKVYLKEFLGYEYDFLGYVALAHVGWVLLFFGVFVYGIKALNFQRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GUL3</accession>
    <name>A0AAD5GUL3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_023613</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000768">
        <property type="protein sequence ID" value="KAI7755845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GUL3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008833">
        <property type="entry name" value="Surf2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47854">
        <property type="entry name" value="SURFEIT LOCUS PROTEIN 2 (SURF2)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47854:SF1">
        <property type="entry name" value="SURFEIT LOCUS PROTEIN 2 (SURF2)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05477">
        <property type="entry name" value="SURF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7755845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="266" mass="29886" checksum="A1C8CC6888D0A312" modified="2024-05-29" version="1" fragment="single">LTNPVFSNPNLRSSPNCVLQESILSNLSNRSKMMNLRSSHRNEAALHFVWSTIGSHDSPEEDDSLSACSLELGDILKPELILPLMETLSLENAASHLQEVGMDQWSVTPGHQSLRCIGCYNWEQSWAQVMQDFCAAVSCSLTVSTQPPSRVYDAAVRKGLLIYHIVQRYKANWLQTSVGCGVCFCLVAQMGPSVTLYREQWMGQPLCCIRVRFHYAAYGSAYAHKLGPKVLSWLSSKLICKLTGHTVNKTEEHIWKHINGKRFLNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GUT5</accession>
    <name>A0AAD5GUT5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Sugar phosphate transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_023408</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005538">
        <property type="protein sequence ID" value="KAI7753131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GUT5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004853">
        <property type="entry name" value="Sugar_P_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050186">
        <property type="entry name" value="TPT_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11132">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03151">
        <property type="entry name" value="TPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="18"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="87"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="132"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="159"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="190"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="Sugar phosphate transporter" evidence="6">
        <location>
            <begin position="17"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7753131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="273" mass="29871" checksum="8570C012B51AD6A5" modified="2024-05-29" version="1" fragment="single">DKSDGVVEKIEAAKRVKIGVYFATWWFLNVIFNIYNKKVLNAFPYPWLTSTLSLAAGSAIMLISWATKVAEAPNTDLEFWKSLFPVALAHTIGHVAATVSMSKVAVSFTHIIKSGEPAFSVLVSKFILGETFPTSVYLSLLPIIGGCGLAALTELNFNMIGFMGAMISNLAFVFRNIFSKRGMKGKSVSGMNYYACLSLLSLLILTPFAIAVEGPKVWAAGWQNAVSEIGPHFIWRSNEERRTKIQEFRGCSVDVGVFTSRIAEHEGGIAACR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GV32</accession>
    <name>A0AAD5GV32_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ubiquitin-like protease family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_002758</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C48 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000267">
        <property type="protein sequence ID" value="KAI7756762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GV32"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.395.10">
        <property type="entry name" value="Adenoviral Proteinase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003653">
        <property type="entry name" value="Peptidase_C48_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02902">
        <property type="entry name" value="Peptidase_C48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50600">
        <property type="entry name" value="ULP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like protease family profile" evidence="4">
        <location>
            <begin position="1"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7756762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="124" mass="14183" checksum="4A659214936E4773" modified="2024-05-29" version="1" fragment="single">MQFDCFKVKWVMLLDLQIKNGQKRDHGICFANPAIISPADRKPKSQHNIENHWVLAVLDMKAATCYYLDSLCPGSVFEPLRQIIDTATTVYSAQTSTGKRFKLRWVNTKCPCQPGFTECGYYVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GX24</accession>
    <name>A0AAD5GX24_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PGG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_005579</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010001144">
        <property type="protein sequence ID" value="KAI7755411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GX24"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026961">
        <property type="entry name" value="PGG_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24177:SF474">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING DOMAIN, PGG DOMAIN, ANKYRIN REPEAT-CONTAINING DOMAIN SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24177">
        <property type="entry name" value="CASKIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13962">
        <property type="entry name" value="PGG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="403"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="441"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="482"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="514"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="69"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="PGG" evidence="3">
        <location>
            <begin position="393"/>
            <end position="506"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7755411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="560" mass="62865" checksum="BAFDF6771A61FE72" modified="2024-05-29" version="1">MERLYEATLQHKFQEIKEILRQGVVTLTDKITMNGNTALHVAVGTTKERKFLKDMLNLATPPLDMQNSEGSTLLHVAAIVGNTEAVELLVEKDSDLLFAMDNEGQTPLARALSNMHTDTYAYLLKNKHSNQDIEAGNGTSWGLLVNIISSKDYGAAKSFFYDNLHMPLTDIDTVLMAIAQNFPSKLNSWERTFVDSTCLYTFLGYQFEALPKFVEAIKRKQEDYRLAKHLLRELCNFIKSKTDSGSHHEYYNNAILEATRQNADEFVRIVVSEFPDAISSTNEHGHNIIQYAVINRSENVYNLLYQMSEHKNMYKTIKDSFGNNLLHLAARLAPPDKLNHISGAALQIQSELQWFKEVQGFVCALNIIQKNSERQTPQMVFTREHKDLVIEGEKWMKETAQSYTITAALITTIVFAAAITVPGGNKQDNGLPEFTSKSAFTVFAISDAISLFTSATSLLMFLSILTARFSEKDFLLKLPTKLMIGLTTLFISTTAMLVAFGATLYLMFCQNNPQILVPVAALTCLPIACFVTLQFPLIKDLMTSTYGHSIFGKKSSIPFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GZT9</accession>
    <name>A0AAD5GZT9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transcription initiation factor TFIID subunit 12 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_018440</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000118">
        <property type="protein sequence ID" value="KAI7757453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GZT9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07981">
        <property type="entry name" value="HFD_TAF12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000011">
        <property type="entry name" value="Transcription initiation factor TFIID subunit 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037794">
        <property type="entry name" value="TAF12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003228">
        <property type="entry name" value="TFIID_TAF12_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12264">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12264:SF21">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03847">
        <property type="entry name" value="TFIID_20kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription initiation factor TFIID subunit 12" evidence="7">
        <location>
            <begin position="302"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="75"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="409"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="54"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="80"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="109"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="161"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="188"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="210"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7757453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="445" mass="47365" checksum="588CBD8E72146A33" modified="2024-05-29" version="1">MSRPPFNRPYPPFPHFNHPTSSPSSSSSITSIPPSQRGGMALGVPAHPSPQPPTSFSSLTSPSFAPQFAALPRSVVPDSLPSTSSPQVRSITGMIGSLGSSSSLRASGVTPSLPQRPLQSSLRPQTSPNNQSAATQNFQDHSHLRHPSVGSAGSPAPPTPQNTQSHNQPWLSSGTQGKPPLPPASFRPQMNPQTLQQRSHIPSQQQTAMSASTPQPQASSSLQPQASSLSQQQQEQQHYSLPPSRVPQALTHQQQMARNRGLGNQRPFAPATGQSSPVTQPPAFNKPPPALEPPETCNRIISKRSIQEIVAQIDPAERLDPEVEDILVDIADDFVESVTTFACSLAKHRKSNTLESKDILLHLEKNWNMSLPGFSGDEIKCYKKPFGNDVHRERLAAIKKSIAAAEAPNLKSSGGQAAGGGAKGHPAKTPGLVIGSPNPKVKETA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BKN3</accession>
    <name>A0AAD5BKN3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_019453</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012117">
        <property type="protein sequence ID" value="KAI7724951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BKN3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7724951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="68" mass="7609" checksum="F39D0062046EC7AB" modified="2024-05-29" version="1" fragment="single">NQRFELGPPGTVYAAAKVTVAMRTATMDEFIIIAVFPFDPNCDPNRNRPIDFTSVFEGSRAFQQSLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BL73</accession>
    <name>A0AAD5BL73_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">WRKY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_020239</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the WRKY group II-b family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011941">
        <property type="protein sequence ID" value="KAI7725418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BL73"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.80:FF:000002">
        <property type="entry name" value="probable WRKY transcription factor 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.80">
        <property type="entry name" value="WRKY domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003657">
        <property type="entry name" value="WRKY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036576">
        <property type="entry name" value="WRKY_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044810">
        <property type="entry name" value="WRKY_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31429:SF106">
        <property type="entry name" value="WRKY TRANSCRIPTION FACTOR 31-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31429">
        <property type="entry name" value="WRKY TRANSCRIPTION FACTOR 36-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03106">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00774">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118290">
        <property type="entry name" value="WRKY DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50811">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="WRKY" evidence="9">
        <location>
            <begin position="244"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="85"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="169"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="109"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="87"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="172"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="190"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00061007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7725418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="504" mass="54655" checksum="55E356E2A9497BCC" modified="2024-05-29" version="1">MGKTEWGLTLESSEPIDMLSPVKTSMYSATVSPPSPPPDHRNDVSEVDFFSTCKSHDDQNIVVKKEINSHVNELDVNTGLHLLTTNSDQSSVDDGGSSSSEDKQGKNKLKILQLELEKMNTENQRLRGMLSQVTNNYTALQIHVANEIQKQQNQKVDHDPEIVAKQLIELRPNSHDSSSDERTQSGSIKNGDHKAAAKRNGRENTPDSDAWVSNKVPKLNGSKIVEQPNESTMRKARVSVRARSEAPMITDGCQWRKYGQKMAKGNPCPRAYYRCTMAVGCPVRKQVQRCVDDQTILITTYEGTHSHPLPPAAIAMASTTSAAATMLLSGSMPSTDGLMNQNILARSILPNSSSIATISASAPFPTVTLDLTHNPNQYQRNPNPNQFVPTMAMPIQAIAADEGVARYNQSRFSGLHLSHDIESRQATPPQYNKGQPQTSFADSVTAATAAITADPNFTAALAAAISTIMNGGNTAATGTSHATTNTATLNHIVNNHKTSNFQGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BLD5</accession>
    <name>A0AAD5BLD5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_016869</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012225">
        <property type="protein sequence ID" value="KAI7724418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BLD5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22160">
        <property type="entry name" value="F-box_AtFBL13-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053781">
        <property type="entry name" value="F-box_AtFBL13-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006566">
        <property type="entry name" value="FBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055411">
        <property type="entry name" value="LRR_FXL15/At3g58940/PEG3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31639">
        <property type="entry name" value="F-BOX PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31639:SF315">
        <property type="entry name" value="LEUCINE-RICH REPEAT DOMAIN SUPERFAMILY, F-BOX-LIKE DOMAIN SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24758">
        <property type="entry name" value="LRR_At5g56370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00579">
        <property type="entry name" value="FBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="44"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="FBD" evidence="2">
        <location>
            <begin position="335"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7724418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="413" mass="48154" checksum="38938BFAC74D2958" modified="2024-05-29" version="1">MCFGNYYVSRNQLLQHYIQQEHTEMKSKRMSKAERSSLDIISTLPQNILETILCRLPTKEAARTSILSREWRYTWTKISKLEFYVLTRIAGQDVGIRLIPFYDILQVLLWHQGLIHEFSLFMYHSTTDQDSFDFDQVVLHLTKNHAVKKLVLQGLNKLSWHKLPKSVFSLHHLTDLDLTYFVLDYQPIFNGFGSRLRSLSLSNVEISTKTLLHLLSNCPSLKTLALFIGESHLGGKDCTVIELFKCLPMIEHLSTFGDVFEWLVLDSVPQGLPTLLLHLKYFCFKNMWFDCGNGLAFLLVLTKCSPNLKKIKLEINFHETCYKEHPAVVWEEYSDVWLEHLNELEIISFGHLKQEIEFVKFILARSPKLSKVSLRSMRCEKERLEMLQALLRAPRASHNLCLTVPLKYARSFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BLL3</accession>
    <name>A0AAD5BLL3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">MYB transcription factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_001672</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011887">
        <property type="protein sequence ID" value="KAI7725647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BLL3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003691">
        <property type="term" value="F:double-stranded telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11660">
        <property type="entry name" value="SANT_TRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000168">
        <property type="entry name" value="Telomere repeat-binding factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005818">
        <property type="entry name" value="Histone_H1/H5_H15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044597">
        <property type="entry name" value="SMH1-6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46267">
        <property type="entry name" value="SINGLE MYB HISTONE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46267:SF15">
        <property type="entry name" value="WINGED HELIX-TURN-HELIX TRANSCRIPTION REPRESSOR DNA-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00538">
        <property type="entry name" value="Linker_histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00526">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51504">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="12">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="11">
        <location>
            <begin position="5"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="H15" evidence="13">
        <location>
            <begin position="112"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="57"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="65"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7725647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="273" mass="30042" checksum="4C7DAA25260FDD38" modified="2024-05-29" version="1">MGNPKQKWTAEEEEALRAGVAKHGTGKWKNIQKDPEFNPYLYSRSNIDLKDKWRNMSVSANGQDSPAATPTPSPMSQAPAGPSALPLLNPVSTDVHMDAAAASKSLLVDGKTASKYDAMIYEALSALKDPNGSDTSAIVSFIEKRHEVPQNVRRHISSRLRRLVSHEKLEKVQNCYRLRRLVVNGTKGPIPKPKEIRPRLSGPINSHLGETLEEAAVAAAYKVAEAENKSFVAAEAVKEAERVSRMAEEAEAFLQLAKEIYERCSRGEVVMLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BMS5</accession>
    <name>A0AAD5BMS5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Strictosidine synthase conserved region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_013899</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the strictosidine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012039">
        <property type="protein sequence ID" value="KAI7725153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BMS5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018119">
        <property type="entry name" value="Strictosidine_synth_cons-reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10426:SF136">
        <property type="entry name" value="PROTEIN STRICTOSIDINE SYNTHASE-LIKE 9-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10426">
        <property type="entry name" value="STRICTOSIDINE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20067">
        <property type="entry name" value="SSL_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03088">
        <property type="entry name" value="Str_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63829">
        <property type="entry name" value="Calcium-dependent phosphotriesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0926">Vacuole</keyword>
    <feature type="domain" description="Strictosidine synthase conserved region" evidence="5">
        <location>
            <begin position="115"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7725153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="287" mass="31155" checksum="7F1218055740C6A8" modified="2024-05-29" version="1" fragment="single">KLVLPPSATGPESAAFDRAGEGPYVTVADGRILKWKGPTVGFVDFAYTSPNRTKKLCDGTNDLEMGPTCGRPIALSFNYKTSDLYITDAFFGLLVVGFNGGLATQLSSGYKYLSGIDVESYTGNVYMTDASMTYNISNMAQPGFTPDSTGRLLKYDPRTQWVTVLMSGLAGAGGPSVSSDRKYVLVPDYVNKKILRYWLQGPKHGTNEVFMTNFGSPNNIKRAVNDGEFWIAVEKQGQGLRVNGSAAVLQTVPFTQFLDTNVAVVQVFNDALYIGSSQTDFVGVYMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BN14</accession>
    <name>A0AAD5BN14_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_031234</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011752">
        <property type="protein sequence ID" value="KAI7726104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BN14"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010052">
        <property type="term" value="P:guard cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000064">
        <property type="entry name" value="Transcription factor MUTE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044283">
        <property type="entry name" value="FAMA/SPEECHLESS/MUTE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46684">
        <property type="entry name" value="TRANSCRIPTION FACTOR FAMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46684:SF1">
        <property type="entry name" value="TRANSCRIPTION FACTOR MUTE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="7">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="62"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7726104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="184" mass="20527" checksum="462E2891D9DF66B7" modified="2024-05-29" version="1">MSHITVERNRRRQMNEHLKVLRSMTPCFYIKRGDQASIIGGVIEFIKEMQLVLELLESKKRRRSISPSPGPSPKPVLGPSTPQSEHSTILHENIKELGASCNSPVADVEAKISGSNVVLRTVSRRIPGQVVKIISLLENLSLEILHLNISSMEDTVLYSFVIKIGLECQLSVEELAIEVQKSFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BQE7</accession>
    <name>A0AAD5BQE7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PPM-type phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_003428</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011339">
        <property type="protein sequence ID" value="KAI7727637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BQE7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="1">
        <location>
            <begin position="29"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7727637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="189" mass="20757" checksum="5606B268E3943DAB" modified="2024-05-29" version="1">MDSLCCLQLRGGPSSYSSGKGKNHEGDIKYGFELVKGKANHPMEDYHVARFVEIDDHELGLFAIYDGHSGDLVPSYLQKHLFDNILNESEFWVDPKRSISKAYEKTDAAILTDDSDLGRGGSTSVTAILMNGQRLWVANLGDSRAVISKGGEAIQLSTDHEPSTERVSIEHKGGFVSNMPGLNCFFQFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BRZ4</accession>
    <name>A0AAD5BRZ4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">E3 ubiquitin-protein ligase FANCL</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_003502</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011396">
        <property type="protein sequence ID" value="KAI7727356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BRZ4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043240">
        <property type="term" value="C:Fanconi anaemia nuclear complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006513">
        <property type="term" value="P:protein monoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23831">
        <property type="entry name" value="DRWD-N_FANCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16490">
        <property type="entry name" value="RING-CH-C4HC3_FANCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.20">
        <property type="entry name" value="RWD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026848">
        <property type="entry name" value="Fancl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026850">
        <property type="entry name" value="FANCL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043898">
        <property type="entry name" value="FANCL_d2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044037">
        <property type="entry name" value="FANCL_d3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043003">
        <property type="entry name" value="FANCL_d3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13206:SF0">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE FANCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13206">
        <property type="entry name" value="UBIQUITIN LIGASE PROTEIN PHF9 FANCONI ANEMIA GROUP L PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11793">
        <property type="entry name" value="FANCL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18890">
        <property type="entry name" value="FANCL_d2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18891">
        <property type="entry name" value="FANCL_d3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01197">
        <property type="entry name" value="FANCL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FANCL UBC-like" evidence="2">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="FANCL UBC-like" evidence="3">
        <location>
            <begin position="75"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="FANCL C-terminal" evidence="1">
        <location>
            <begin position="155"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF11793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF18891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7727356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="235" mass="26683" checksum="8B8FDBCA6891202D" modified="2024-05-29" version="1" fragment="single">IEEIGWEHLLKLAEDLSDKKERVHVLEILLDKSYPNCPPSVSADVPYLFDLEWSRNSRLNDVVKQFRQHLEKLQQFWSAVEDIDQSLWVSDSAQLHRATSFRHINIGNDCSIMLHIRANEPRSLPESKDKLFAENLANLLDFQLPGPPGVEKNDQQLDCGICYSQFLPVDDELGPKSGGTTDYVCENTTCSKAFHSVCLGDWLRSITTTRQSFDVLFGNCPYCSQPVAVKIGTRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BSM2</accession>
    <name>A0AAD5BSM2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP-dependent DNA helicase</fullName>
            <ecNumber evidence="1">5.6.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_025563</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.3" evidence="1"/>
    <dbReference type="EMBL" id="JAMZMK010011385">
        <property type="protein sequence ID" value="KAI7727414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BSM2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010285">
        <property type="entry name" value="DNA_helicase_pif1-like_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025476">
        <property type="entry name" value="Helitron_helicase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049163">
        <property type="entry name" value="Pif1-like_2B_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10492">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10492:SF94">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14214">
        <property type="entry name" value="Helitron_like_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05970">
        <property type="entry name" value="PIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21530">
        <property type="entry name" value="Pif1_2B_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0227">DNA damage</keyword>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="1" id="KW-0234">DNA repair</keyword>
    <keyword evidence="1" id="KW-0347">Helicase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helitron helicase-like" evidence="4">
        <location>
            <begin position="336"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="domain" description="Helitron helicase-like" evidence="4">
        <location>
            <begin position="404"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="domain" description="DNA helicase Pif1-like DEAD-box helicase" evidence="3">
        <location>
            <begin position="897"/>
            <end position="1103"/>
        </location>
    </feature>
    <feature type="domain" description="DNA helicase Pif1-like 2B" evidence="5">
        <location>
            <begin position="1194"/>
            <end position="1237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="305"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="36"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="49"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="310"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF21530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7727414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="1264" mass="145047" checksum="063856D52E44C19B" modified="2024-05-29" version="1" fragment="single">CDPYLEPHLHLDMDMPPREHKDVAGTSSHKQHYPGPSTSSALQTPYNKRCNDSHDATRRLRTTSRRILVEQGNNTNSHLDESILTPTSTLHLDIGIPQIDHNIVPDFLPDRQSLPPDYVPISDDTPIRQDNNITQAGTSYLDIDLNMPPPDHDDAPEDSEDNELDARLLWPNLDRNITEVLTRVLSNNPYVKTFRSLADLGPLDNYRVTLNASVELDQRVYNRPTTSEVAGIWVEGNDDITSYKRSIVVYGRSGQPQTIQPYLSCYDPLSYPLFFPHGEPGWHSGIPRQGVEANATFEANVTFEANATDNEQESDEDNTEEETKRRNRTTVSMREYYCYRFQIRPTQNILLLGGRLLQQFTVDTYIKLETARLQYYHHNQTKIRADLYQGIVDCINAGDAQPNRIDLVARVFRAKLEELKKQLFQKHILGEVKAYVYVIEFQKRGLPHAHFLLIMYPHHKVINPDQYDQFVSAEIPSMERFPQLHEIVVKHMIHGPCGPLRTNSPCMQGTPKLCRFRYPRQFNDQTRQGEDAYPLYRRRNNGIRVDVRGKTLDNRWVVPYNPTLLMLFNCHINVEICSSIKSVKYLFKYIYKGHDKQVIQVEPDAENVIIDEIRRYQDARYVSPPEAMWRIFSFTLSQIQPTVLTLQLHLPDKQMVTFTEKSNMRNVVKKERNKKTMLTAFFEENRLHPEARNHLYKDFPKHYTWSTKKRCWKPRARIPQKGRIVTANPAEGERYYLRLLLTSVRGPTSFEHLRTVNDVLHATFRKAAQELGLIENDDILSQCLEEASIFQFPNALRRLFATILIFCEPGDVRQLWSSHFDALSEDHRLHSQNMTHVQNLVLTEIETHLQSMGKSLNDYDLPRPSQPLHNQHTTHRDIQEECSIVVHPTDLQAKNLLNCDQKRAFDEIMRHINHAIPAVFFIDGPGGTGKTFLYKALLAEVRSHGHVALATASSGAAANNMPGGRTAHSRFKIPTKLDNNSLCGIEKQSGRAQLIRLAKLIIWDEASMARRQSIEAVDRTFQDITGINLPFGGKIMVMGGDFRQVLPVIKRGTRAQIVDASLRMSPIWSVTKKVRLSINMRALTDPWFSEFLLRVGNGTEETTDGNQIRIPDDMTIPFTTKENSIKQLIKSIFPSLNSGAQSSDYITSRAILTTRNESVDEINNQMIDIFQGEERHYYSFDEVDDDQHHLYPIEFLNSLNVSGIPPHKLRLKVGCPIILLRNIDPSNGLCNGTRLICKRFERNVIDAEIAVGEHKDLQGMVDLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BT71</accession>
    <name>A0AAD5BT71_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Mediator of RNA polymerase II transcription subunit 19a</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_015003</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011275">
        <property type="protein sequence ID" value="KAI7728048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BT71"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019403">
        <property type="entry name" value="Mediator_Med19_met"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22536">
        <property type="entry name" value="LUNG CANCER METASTASIS-RELATED LCMR1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22536:SF12">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 19A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="101"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="127"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="150"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="181"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7728048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="199" mass="23056" checksum="41CDCE5ED5B059CF" modified="2024-05-29" version="1" fragment="single">MDPESKTFGKGPRELTGAVDLISYYKLLPHHEFFCKKSLPLSISDTHYLHNVVGDSEIRKGEGMQLDQLISNDSFSRDTNTRIQPFDLDILREAFQLRESAPVDLPSSEKGTPTVAGKSKSESKDKEKKHKKHRDKDKEHKKHKHRHKDRSKDKDKEKKKDKSSHDKKRKHDGDEDLNDIHRHKKSKHKGSKMDEMGTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BTH6</accession>
    <name>A0AAD5BTH6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">PGG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_018639</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011012">
        <property type="protein sequence ID" value="KAI7729378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BTH6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026961">
        <property type="entry name" value="PGG_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24186:SF37">
        <property type="entry name" value="PGG DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24186">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13962">
        <property type="entry name" value="PGG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="285"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="330"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="358"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="396"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="70"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="105"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="PGG" evidence="9">
        <location>
            <begin position="278"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7729378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="444" mass="49199" checksum="3390E18B921CD6E0" modified="2024-05-29" version="1" fragment="single">METKLYEASLTGDVQTLNALLHQDQLILDRLSLTIFNESPLHIAAMRGHHQFVIALLTQNPKLALALDTQRRTPLHLASANGYLETVQELIRAGGRDVCCFRDQDGLTPLHLAAMNEHVEVVKALVQANADAAKVIAATGETILHMCVSYNRLESLKVLMQLWTEEELAKIIDNGGNTLLHVAAINKQTQILNYLLGIPSIKDNGNAVNKHGLTALDVLDQCPRDLKSLETREILMEAGVLRASDLGLPSLPKPSTKSSHNRRKGLGIWARYVNDDHHWIEQQRGILIVAALLVAGISFYSGINPPNNILSNNTNGRFLFQSEMDDFNQFVVLNSLTMATSLAIVLVLISGLSLGNKFWIWVLTLGTLFIMGAMVSNYWAQLSNFGPDGYVDDASLWILLVFLFGCGLFGLIHTIFFVVWVVMKLLKMGKMPETNRKRNQDDAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BTN3</accession>
    <name>A0AAD5BTN3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosomal protein L19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_010260</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011006">
        <property type="protein sequence ID" value="KAI7729422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BTN3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1650.10:FF:000001">
        <property type="entry name" value="Ribosomal protein L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1650.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035970">
        <property type="entry name" value="60S_ribosomal_eL19_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039547">
        <property type="entry name" value="Ribosomal_eL19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023638">
        <property type="entry name" value="Ribosomal_eL19_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000196">
        <property type="entry name" value="Ribosomal_eL19_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015972">
        <property type="entry name" value="Ribosomal_eL19_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057259">
        <property type="entry name" value="Ribosomal_L19e"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10722">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01280">
        <property type="entry name" value="Ribosomal_L19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01416">
        <property type="entry name" value="Ribosomal_L19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48140">
        <property type="entry name" value="Ribosomal protein L19 (L19e)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00526">
        <property type="entry name" value="RIBOSOMAL_L19E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein eL19" evidence="5">
        <location>
            <begin position="3"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7729422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="88" mass="10131" checksum="0AB05BF357B3E31C" modified="2024-05-29" version="1">MVSLKLQKRLAASVLKCGRGKVWLDPNEGNEISMANSRQNIRKLVKDGFIIRKPTKIHSRSRARRMKEAKRKGRHSGYGILRFLRISS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BTP0</accession>
    <name>A0AAD5BTP0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_019442</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011071">
        <property type="protein sequence ID" value="KAI7729104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BTP0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02947">
        <property type="entry name" value="TRX_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000001">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45663">
        <property type="entry name" value="GEO12009P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45663:SF21">
        <property type="entry name" value="THIOREDOXIN M3, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Thioredoxin" evidence="6">
        <location>
            <begin position="41"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7729104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="163" mass="18338" checksum="5FABA4F37F496FC4" modified="2024-05-29" version="1">MAAASCTYSLVTRRYCSIPQLKPSIAQDQLKTSNDLSFRVPKNRSCLPVLSMRQESAVLEVTPKSWNPSILKSETPVLVEFYTSWCEPCQMVHQMINEIATDYAGRLECVAVDVEQEPRVAEQYDIKAVPIVVLFKNGEKCESVVGTMPKDFFVDAIERVLDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BUU3</accession>
    <name>A0AAD5BUU3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_023805</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011132">
        <property type="protein sequence ID" value="KAI7728826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BUU3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031519">
        <property type="term" value="C:PcG protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000071">
        <property type="entry name" value="Putative zinc finger protein 143"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000221">
        <property type="entry name" value="Zinc finger protein 410"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000818">
        <property type="entry name" value="zinc finger transcription factor YY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001190">
        <property type="entry name" value="zinc finger transcription factor YY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR PROTEIN YY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003:SF1">
        <property type="entry name" value="ZINC FINGER TRANSCRIPTION FACTOR YY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="79"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="108"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="138"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="168"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="239"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="265"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="335"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="265"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="292"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="345"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="371"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7728826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="381" mass="43876" checksum="49482DC87D8ADD22" modified="2024-05-29" version="1">MEHMNISFSNPFERRTFTNPKTPAVKWFKEWVPQDVVTTGGKCLVLKWVTESQLKAIKEKPKEVEPVSSEPEPTTEVLFLCSYEGCGKTFIDAGALRKHSHIHGDRQYVCHYENCGKKFLDSSKLKRHFLIHTGERDFVCPHEGCGKAFSLDFNLRSHMKTHSQENYHLCPYPGCGKRYAHEYKLKNHIMSHHEKQNNNNMMVEAPVKYIHIQPAEKQPQKSTKASTTSYASASSVRPYACPYEGCDKAYIHEYKLNLHLKREHPGHFPEENTKNAHENEMDAGSDHDGHVIKRGNLKSHKHSRPKPDFKLPPAKVIKQQAPFVNQALTSVVNKQPWATGKDLHEEEDSEETEEDRDDGEAQWGYGNVNNYEDDEETEYED</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BWK6</accession>
    <name>A0AAD5BWK6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HTH myb-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_014320</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010922">
        <property type="protein sequence ID" value="KAI7729788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BWK6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH myb-type" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="48"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="82"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7729788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="363" mass="40427" checksum="13B63EC3EA18993A" modified="2024-05-29" version="1">MNLTDNAIKNHWNSSLKKKLDFYTATGNLSFLSKYDIDGVGDISKTPSTKPIAAPHTTAWESPAATEQCKEETDTTYDIECSTPSEDVDPSSSFIPSGSTDSEPIGTKSQRSKFDLSHLSRNLISKFDDCTTLQNGTNLQRLNIAPLQSNIRTYGSLCYEPPQSGIYTPWNSYEHNSNSFIMSPKCSPTPPYAEKCGSLYAQTPESILKLAAKSFPNTPSILRKRKVQSPMSFQKIVKGDEVKEANNDLKESEPCVGGDSIGGNGAEGGVTAVSAYNASPPYRLKYTRKSNLKSVEKQLKFRFDEETCKSETQSEDLNVKDNSESWIALRYMKLSIKDCCHVVPNRMRPKHMFHLMNKEIKDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BYG0</accession>
    <name>A0AAD5BYG0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="9">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_011212</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PPP phosphatase family. PP-1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="9"/>
    <dbReference type="EMBL" id="JAMZMK010010370">
        <property type="protein sequence ID" value="KAI7731837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BYG0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07414">
        <property type="entry name" value="MPP_PP1_PPKL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000026">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050341">
        <property type="entry name" value="PP1_catalytic_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031675">
        <property type="entry name" value="STPPase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668">
        <property type="entry name" value="SERINE/THREONINE PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668:SF463">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16891">
        <property type="entry name" value="STPPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0464">Manganese</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="10">
        <location>
            <begin position="117"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7731837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="373" mass="42165" checksum="800175C2E85C7BF6" modified="2024-05-29" version="1">MDQSVLDDIIQRLLAVKGRPGKQVQLTEQEIKQLCLVSKDIFLQQPNLLEIEAPVKICGDIHGQYSDLLRLFEYGGLPPQANYLFLGDYVDRGKQSLETICLLLAYKIKYPNNFFLLRGNHECASINRIYGFYDECKRRFNVKLWKTFSDCFNCLPVAALIDEKILCMHGGLSPDLNNLDQIKNLRRPTDVPDSGLLCDLLWSDPCNEVKGWGMNDRGVSYTFGADIVTEFLEKHDLDLVCRAHQVVEDGYEFFANRQLVTVFSAPNYCGEFDNAGAMMSVDETLMCSFQILKPAEKKLKANSGISLFGSTTTAKPGNTPAGIKATTNLNLCCCLFYGTLYVAFAAVSGWKIMRFGVEWRLKQMKLHWFLVHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C094</accession>
    <name>A0AAD5C094_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_012807</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010409">
        <property type="protein sequence ID" value="KAI7731661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C094"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="449"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="450"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7731661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="480" mass="54804" checksum="A9D676FE43D8D336" modified="2024-05-29" version="1">MCIPRTQKKGGDKQSVDFVKGLSNTFLPDLSTPYPNHSKKPPVHLFSSSFPYSSNVFVLSLGLLEHGHVMVAEVPGNRHHEWLHFEAHEEEYEKRLDALWPRRHLILAGRRLDFATMNILGQKKRWDDIVTGPWRKLFRIHQVQYVELVMEFFATYEFPNPNQDLQDSRAIRFRLGGRWMEISVARLGEILGLYTAEEIDSELFTAGIIDFPSERVKKEFWADQGVGNYTPGKTKASKFRDPLLRILHRCLVYSIAGRFKSETAINLRELFYLYCLVHPHTCNLAHVIAEYFLTSSGREATSAIFGGHLVTKIARSYELLTDEALKKLTPVAETQRMNIKTLHHMRLVKKTQMGKRLVDRWGRIWNPLRPGDDPDRRQEAQVQVVGEVAGQESHVGAAPVIPPPSPQQDEVIIPLSERRILAWMYETNLAWAAAAKAAGIHVQIPVPFFMHPPPQPPQPPVDREQHTQDGDLGGMDLDAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C0X2</accession>
    <name>A0AAD5C0X2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dienelactone hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_028420</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010267">
        <property type="protein sequence ID" value="KAI7732263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C0X2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002925">
        <property type="entry name" value="Dienelactn_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051049">
        <property type="entry name" value="Dienelactone_hydrolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46623:SF6">
        <property type="entry name" value="ALPHA_BETA-HYDROLASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46623">
        <property type="entry name" value="CARBOXYMETHYLENEBUTENOLIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01738">
        <property type="entry name" value="DLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dienelactone hydrolase" evidence="1">
        <location>
            <begin position="18"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7732263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="238" mass="25734" checksum="41D561E9A39BAF6C" modified="2024-05-29" version="1">MATSDLVKIQIQRENITFDAYVVGKDDAPGIVVIQEWWGVDFEIKNHALKISQLGYKALIPDLYRGKVGLDAAEAQHLMDGLDWPGAVKDIQASVNWLKANGSQKVGVTGYCMGGALTIASSVLVSGIDAGVAFYGVPPPQLADPAEAKLPIQAHFGELDNLVGFSDITAAKALEERLKASGTPYEVYVYPGVTHAFMNTSPEGNARRKGMGMIDDNEAAADLAWSRFQTWMNRYLSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C258</accession>
    <name>A0AAD5C258_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_031746</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009949">
        <property type="protein sequence ID" value="KAI7733535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C258"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7733535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="71" mass="7955" checksum="C0E3AE5E22E5AA97" modified="2024-05-29" version="1" fragment="single">SVVNCAIIKGLTRFRCVSAVHKLGVDNGCYDLRNNARKICAQLKTTTNTTDRTKYGVIWAKPRIRRSITST</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C338</accession>
    <name>A0AAD5C338_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_031937</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009888">
        <property type="protein sequence ID" value="KAI7733910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C338"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7733910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="132" mass="15242" checksum="E075144195A03951" modified="2024-05-29" version="1" fragment="single">MIGFSSFAKLKGLTVVFNNSAYDDEDDDDLPCDVVDFGKKEKEFGGSSCSSSYLNEEHITSSFMRCFLYPSKVRKDIWLQYDSLANPRTKTTDFWFRSEVTCPVPGRSWWLRTIVHINRFFGFAGTYVVLFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C5J1</accession>
    <name>A0AAD5C5J1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_033021</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009488">
        <property type="protein sequence ID" value="KAI7735420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C5J1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050869">
        <property type="entry name" value="H3K4_H4K5_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12197:SF298">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE ATXR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12197">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SMYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SET" evidence="1">
        <location>
            <begin position="36"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7735420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="300" mass="33176" checksum="2DA86CF246BC3B7D" modified="2024-05-29" version="1" fragment="single">RLAHQCFPQTNLHLASYFFSTTSATNHHRGSNPSPPPIQVLLTESAGRGVFATRSIQPGELIHTAKPLVSHPTISSIDKGFYEVEKEADWSAFVAHCRARSLKYPFLAKRLACMVISGVTAASSLDILQPARLSAEMISQMEEELCLLRGALEGSGMKDEQLTFLNIEWYAGVLARIRINAFRIELAGNIYEDLLALAAASVESEAAVGNAVYILPSFYNHDCDPNTHIVWVENVEARLKALREIEAGQVKNCGYVTLMQAWIMMLDKISCPVGLVLSVIVLDLEREHGAFLLKFEFKAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C5M8</accession>
    <name>A0AAD5C5M8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cullin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_021706</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cullin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009624">
        <property type="protein sequence ID" value="KAI7734903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C5M8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1310.10">
        <property type="entry name" value="Cullin Repeats"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045093">
        <property type="entry name" value="Cullin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001373">
        <property type="entry name" value="Cullin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016159">
        <property type="entry name" value="Cullin_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11932">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00888">
        <property type="entry name" value="Cullin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74788">
        <property type="entry name" value="Cullin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cullin N-terminal" evidence="2">
        <location>
            <begin position="51"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7734903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="298" mass="34368" checksum="58FF35BC92705D29" modified="2024-05-29" version="1">MKRRLTLEEGMSVLKEGISKAMMILDGHPTGSLFTCEEYMKLYDCSLSFTLASCVYHMCVQQPPYEYCAELYDIFKKALEESITSRVMPALKDKAEIILLYEVWNMWTKYKVMAKFLGGFFLYLDRHFTEDRNTASLNELSICCFRDLVFNELYSKILEAAISLINQDREDNSVCRDLLKNVSTLFIEIGSGKMDYYDNFETAMLASTANYYNHLLRQWLMHYSPMDIFLKVVHEQLLGQAALDQAKLYEKQKAEYGDCAIQYQACFILLLNFRTALCLSIRVLLLGGAVGVCGFESR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C6N8</accession>
    <name>A0AAD5C6N8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_024130</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase A1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009330">
        <property type="protein sequence ID" value="KAI7736217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C6N8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032799">
        <property type="entry name" value="TAXi_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032861">
        <property type="entry name" value="TAXi_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13683">
        <property type="entry name" value="ASPARTYL PROTEASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13683:SF810">
        <property type="entry name" value="NEPENTHESIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14541">
        <property type="entry name" value="TAXi_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14543">
        <property type="entry name" value="TAXi_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase A1" evidence="2">
        <location>
            <begin position="1"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7736217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="262" mass="27310" checksum="7A65F3BA970FD61F" modified="2024-05-29" version="1" fragment="single">CSTAQTGDLTKSDRAVNGIFGFGQSGLSIISQLSSQGVAPNAFSHCLIGSDDGGGILVIGQIVEPNMSISVNGQTLSIDPSIFAISDNQGGTIVDSGTTLSYLAEDALNPFVDAITQSVSQSVQPLISKKANKCYIITSSVSDVFPPVSFNFEGGASMHLRPQDYLLQQNSVGGAEVWCIGFQSISNQGITILGDLVLKDKIIVYDLGGSSSVNVSLASNGGRNEVVNAGQVGGSISLQITRYELIPIFILGFILHLAGTYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C6U4</accession>
    <name>A0AAD5C6U4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_011010</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009550">
        <property type="protein sequence ID" value="KAI7735139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C6U4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006461">
        <property type="entry name" value="PLAC_motif_containing"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01571">
        <property type="entry name" value="A_thal_Cys_rich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15907">
        <property type="entry name" value="DUF614 FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04749">
        <property type="entry name" value="PLAC8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7735139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="153" mass="17004" checksum="A42E33217138454E" modified="2024-05-29" version="1">MTTPSHWSTGLCDCGTDCSSCCLTCWCPCITFGRIAEIVDKGTTSCGVHATLYSLLCLLTGCATPYSLLCLLTGCECIYSCMFRSKLRQQYNLPEEPCNDLCVHWCCKPCAHCQEYRELKHRGFEPSLGWEGNLARQSQGVVMPPVGPNEMKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C761</accession>
    <name>A0AAD5C761_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">mitogen-activated protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_027195</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.24" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010009239">
        <property type="protein sequence ID" value="KAI7736563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C761"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004707">
        <property type="term" value="F:MAP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000017">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000046">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003527">
        <property type="entry name" value="MAP_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01351">
        <property type="entry name" value="MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="78"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7736563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="366" mass="41742" checksum="0AB10FB1C14F84EB" modified="2024-05-29" version="1">MGGGTFVDGVLRWFNHRRSSSSAAAAAVESKHVFDLSSLNLITVPKRTPFPSTMDHTTTNKQQLGAEFFTEYGEASRYQVQEVIGKGSYGVVASALDTHTAEKVAIKKINDVFEHVSDATRILREIKLLRLLKHPDIVQIRHIMLPPSRREFQDIYLVFELMESDLHQVIKANDDLTPQHHQFFLYQLLRGLKYIHTANVFHRDLKPKNILANSDCKLKICDFGLARVSFNDAPSTIFWTDYVATRWYRAPELCGSFFSKYTPGVDIWSIGCIFAEMLLRKPLFPGKNVVHQLDLMTDLLGTPSSESIARIRNEKARRYLSNMCKKPPVPFAHKFPNVDPLALRLLQRLLSFDPKDRPSAEEVPML</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C7T8</accession>
    <name>A0AAD5C7T8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">UGP3-like C-terminal hexapeptide repeats domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_006024</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009449">
        <property type="protein sequence ID" value="KAI7735694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C7T8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003977">
        <property type="term" value="F:UDP-N-acetylglucosamine diphosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006048">
        <property type="term" value="P:UDP-N-acetylglucosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057388">
        <property type="entry name" value="Hexapep_UGP3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039741">
        <property type="entry name" value="UDP-sugar_pyrophosphorylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002618">
        <property type="entry name" value="UDPGP_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11952">
        <property type="entry name" value="UDP- GLUCOSE PYROPHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11952:SF14">
        <property type="entry name" value="UTP--GLUCOSE-1-PHOSPHATE URIDYLYLTRANSFERASE 3, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25441">
        <property type="entry name" value="Hexapep_UGP3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01704">
        <property type="entry name" value="UDPGP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="UGP3-like C-terminal hexapeptide repeats" evidence="3">
        <location>
            <begin position="705"/>
            <end position="839"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7735694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="853" mass="95076" checksum="9E4A06CD197D1206" modified="2024-05-29" version="1">MAQATTSSLHHNGLYFTFSARDSHALFFNPHKSHRPSNKLPFTHLSFIPSPPSNRRTTLVSTVPVEHVPAPDFNFTEELTRLRTLRSKLYKTKTLKDKIHVLNSDSNVKRFFKSDYYYYYKKGFARVLEALGLDEYELFLLKCIVAAGQEHVLVSGYGTDLDVKDVKGEFVSARSSLKSALYSLVEMIEKWDGSDDGEVRVNEIDAVALNSLLKILRDVDEFYDCIGGIIGYQLTVLELLSQSPHGEKIANFQNLDNSNSMGMQFVEIHPPSVLDLSQDTEYAAQAALWGIEGLPSLGEIYPLGGSADRLGLVDSVTGECLPAAMLPFCGRTLLEGLIRDVQAREFLYFKLFGEQCITPVAMMTSSAKNNHEHITLLCERSKWFGRGRSNFRLFEQPLVPAVSAEDGQWLAKGQFLPVCKPGGHGVIWKLAYDKGVFQWFYDHGREGATVRQVSNVVAATDVTLLAMAGIGLHEGKKLGFASCKRNSGATEGINVLVEKGKPDGTWTYGVSCIEYTEFDKFGIADGSSSNSLQTEFPANTNILYVDLKAAEHIASSKDESSLPGMVLNVKKSINFVDHFGLQHGVPGGRLECTMQNIADHLVNGFSTRYTEGVEEMLDTFIVYNHRRKVTSSAKKKRKPAGMSLHQTPDGALLDTIRNAYDILSYCDIKLPEIESNDKYADSGPPYIILLHPALGPLWEVTRQKFHGGSISKGSELQIEVSEFLWRNVQLDGSLIVMAENVMGSHVDRNHESILQYGYRCARCKLVNVNIQNKGIDWSSDKNLYWKHDVLRFEALKVILHGNAEFEAVDVNFEGNHVFEVPDGYKMMVTSGSSGFLTASHQYFCASMFVFINT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C9E7</accession>
    <name>A0AAD5C9E7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_003959</name>
        <name evidence="10" type="ORF">M8C21_003961</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008974">
        <property type="protein sequence ID" value="KAI7737655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAMZMK010008974">
        <property type="protein sequence ID" value="KAI7737657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C9E7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000011">
        <property type="entry name" value="Myb transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000174">
        <property type="entry name" value="MYB-related transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044676">
        <property type="entry name" value="EOBI/EOBII-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45675:SF98">
        <property type="entry name" value="HOMEODOMAIN-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45675">
        <property type="entry name" value="MYB TRANSCRIPTION FACTOR-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Myb-like" evidence="7">
        <location>
            <begin position="8"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="8">
        <location>
            <begin position="12"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="8">
        <location>
            <begin position="61"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="7">
        <location>
            <begin position="61"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7737655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7737657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="228" mass="26928" checksum="B31B4416344DDD60" modified="2024-05-29" version="1">MYWGGVHEEKWRKGPWTTEEDRLLIHHVNLHGEGRWNSVSTLSGLKRNGKSCRLRWVNYLRPDLKRGRITPHEETIILDLHARWGNRWSTIARSLPGRTDNEIKNYWRTHFKKKSKVMDESTKIKTRLLKRQKFQQQQRQLQLQHLHMQQNGISLVEESENYKVGYMSPTMVVRQQDKVYDQEQQISLLLNGGGGCYVPQTPTNEDVGLWDGLWNLDDIHAPDFDQLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C9T7</accession>
    <name>A0AAD5C9T7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">M8C21_005885</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="23">AA19_3_7</strain>
            <tissue evidence="23">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010008962">
        <property type="protein sequence ID" value="KAI7737752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C9T7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048638">
        <property type="term" value="P:regulation of developmental growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000021">
        <property type="entry name" value="Putative LRR receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000015">
        <property type="entry name" value="Somatic embryogenesis receptor kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000016">
        <property type="entry name" value="Somatic embryogenesis receptor-like kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47988">
        <property type="entry name" value="SOMATIC EMBRYOGENESIS RECEPTOR KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="14 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-0675">Receptor</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="239"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="22">
        <location>
            <begin position="302"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="587"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="330"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="KAI7737752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="621" mass="68495" checksum="EA1F4679DAF9560C" modified="2024-05-29" version="1">MRVQANIHLFSFIFLWFLVCSNALLSPKGVNFEVQALMGIKASLLDPHNVLENWDADAVDPCSWTMVTCSSESLVIGLGTPSQNLSGTLSPSIGNLTNLQIVLLQNNNITGSIPKEVGKLKMLQTLDLSDNHFTSEIPPSLGHLTALQYMRLNNNSLSGTIPESVANMTQLAFVDLSFNNLSGPVPKFPSKTFNIVGNPLMCPKGFEQQCYGQTLMPISMPLNTTQTAKTSSRRKTHKVAIAIGTSLGCSFLLVFGLALLWWTRRHDQGPFFDVKDKHHEEVLLGNLRKFQFRELQIATHNFSNKNILGKGGFGHVYKGTLQDGTLVAVKRLKDGGAAGGERQFQTEVEMISLAVHKNLLRLYGFCMTPTEKLLVYPYMPNGSVASRLKAKPVLDWGIRKKIALGAGRGLLYLHEQCDPKIIHRDVKAANILLDDCCEAVVGDFGLAKLLDHQDSHVTTAVRGTVGHIAPEYLSTGQSSEKTDVFGFGILLLELITGQRALEFGKAANQKGAMLEWVKKINHEKKLDILVDKDLKNNYDMVELEELVKVALLCTQYLPGHRPKMSEVVRMLEGDGLAERWEASQGIESTGKYKMPETSSSERYSDDDSSLLGQAIELSGPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CAV0</accession>
    <name>A0AAD5CAV0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">GATA transcription factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">M8C21_007362</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">AA19_3_7</strain>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Transcriptional activator that specifically binds 5'-GATA-3' or 5'-GAT-3' motifs within gene promoters.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the type IV zinc-finger family. Class A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009072">
        <property type="protein sequence ID" value="KAI7737269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CAV0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00202">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000018">
        <property type="entry name" value="GATA transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051140">
        <property type="entry name" value="GATA_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016679">
        <property type="entry name" value="TF_GATA_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45658">
        <property type="entry name" value="GATA TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45658:SF41">
        <property type="entry name" value="GATA TRANSCRIPTION FACTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00320">
        <property type="entry name" value="GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016992">
        <property type="entry name" value="TF_GATA_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00401">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00344">
        <property type="entry name" value="GATA_ZN_FINGER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0010">Activator</keyword>
    <keyword evidence="7 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 11" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GATA-type" evidence="14">
        <location>
            <begin position="239"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="172"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="180"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR016992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAI7737269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="321" mass="35408" checksum="7AC7D3834649BB7B" modified="2024-05-29" version="1">MECIEARALKSSFLSKIIGMKKIQQASIEDFLCVAGINSVVNVSSDEFSIDDLLNLPDKDFSNPDTDSFEELSEEDFASVSSQDNDSGSMNSSMFSSTGDLVSLSVDQLSVPIDDMENLEWLSQIVDDSVPELPMLCPPVNRFEPVVNVSSRSFTVLGLPYPVPKKCRTDRSRKAGRVWSAGSLTESSSSSSSSHDSSITSPILFKIPFYSMEVFDKQPAAKKPRKSPACQLGRGSSDSLSQRRCTHCQVQKTPQWRTGPLGPKTLCNACGVRFKSGRLFPEYRPACSPTFSGDIHSNSHRKVLELRKKEKEVELVLNMTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CBN6</accession>
    <name>A0AAD5CBN6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_020981</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009005">
        <property type="protein sequence ID" value="KAI7737496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CBN6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36324">
        <property type="entry name" value="OS09G0460100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36324:SF1">
        <property type="entry name" value="OS09G0460100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7737496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="221" mass="25446" checksum="D7998FE527E99903" modified="2024-05-29" version="1">MIMGLFHHQEPASNPSKKYRFFKDALATCHTFCRKISSHSLQQHDDHGDDDEDVIDYDDEQEIFVSAVLSKYKESKSKRKVGLTVDSFIWELEFELGLGNLSPTEPRLTRKEIHHAYASDVEDHRPEEFYSPCSHLSRCSSDTTAAGAFVSVKTNLSRCSSMSSFEFPVFKRRRRSMLLELCHCEGWPFGLCRKALLVPPLPKSPSESWMWRKSSRLVKIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CCL0</accession>
    <name>A0AAD5CCL0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">BRO1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_002048</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008700">
        <property type="protein sequence ID" value="KAI7738885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CCL0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.280">
        <property type="entry name" value="alix/aip1 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004328">
        <property type="entry name" value="BRO1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038499">
        <property type="entry name" value="BRO1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23030">
        <property type="entry name" value="PCD6 INTERACTING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23030:SF30">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE NON-RECEPTOR TYPE 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03097">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01041">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51180">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BRO1" evidence="1">
        <location>
            <begin position="13"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7738885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="291" mass="32551" checksum="CDB7A0C3BFA3CE18" modified="2024-05-29" version="1" fragment="single">MSSPPSSSSSTNIMLAIYEKKTTTTDLYRPLRNYITFNYSEHEAQTLDDDLQTLNQMRTTIERSSPIDSLTSRRDLLQNYFKTLTLVESRFPISNNKEDINTVTFTWYDAFKSKQKAVQQNVHLEKAAVLFNLGAVYSQIGLGFDRMSVEGRREAVKAFVAAAGAFAFLKESEAGKASGGDLKTTVDVSVECAGMLERLMLAQAQECVFENSIAKGSSSGVCSKIARQVGLYYEEVLAALNVVPLTQHFDKAWLSHVQLKAALFYAEACYRYSLELHEKEEIAEEIARLKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CDH9</accession>
    <name>A0AAD5CDH9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Root phototropism protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_031600</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the NPH3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008811">
        <property type="protein sequence ID" value="KAI7738471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CDH9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000168">
        <property type="entry name" value="BTB/POZ domain-containing protein At1g03010"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043454">
        <property type="entry name" value="NPH3/RPT2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027356">
        <property type="entry name" value="NPH3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32370">
        <property type="entry name" value="OS12G0117600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03000">
        <property type="entry name" value="NPH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51649">
        <property type="entry name" value="NPH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="BTB" evidence="6">
        <location>
            <begin position="8"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="NPH3" evidence="7">
        <location>
            <begin position="163"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="527"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="533"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="550"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7738471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="559" mass="62490" checksum="7C0F9E69500E6D63" modified="2024-05-29" version="1" fragment="single">VFSQDIPADILIQVGEAKFPLHKFLLVAKSNYIRRLILESKEPDMTRLDLSNIPGGAEIFEKAVKFCYGVNFEITVHNVAALHCAAEYLEMTDEYCDGNLAGRTDDFLVQVALTSLSGAVVVLKSCEDLLPIAEQINIVQRCVELASAKACNEANFPSRSPPNWWTDELSIVHINFFKQIIASMKSRGAKPLTIACAIITYTERALPDLVRDRSGNTITTSVSIDSTTSKFQQRELLSAIVSLLPVETQRASFPINFLSCLLRAAIYLENDDVCKKQLEKRISAILEHVTVDDLLVLSYTFDGEQLFDLESVRRIISGFVEKEKSVSVFNGGDFREPSSSTAMLRVAKTVDAYLGEIATIPELSISKFNGIANLVPKNARKIDDDLYRAIDIYLKAHPNLDEIEREKVCSAMDPLKLSYEARVHASQNKRLPVQIVLHALYFDQLQLRSDKSERSTPTPNAISMRSQVQADVSLAKENEALRTELLQMKAYISDIQNKNQVGSTSARISNPKKPTFFSSVSKKLGKLNPFKHGSKDTSHIEDGNDIDHTKPRRRRFSIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CED2</accession>
    <name>A0AAD5CED2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">M8C21_000491</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">AA19_3_7</strain>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010008574">
        <property type="protein sequence ID" value="KAI7739725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CED2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12122">
        <property type="entry name" value="AMPKA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14079">
        <property type="entry name" value="STKc_AMPK_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000003">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001222">
        <property type="entry name" value="Serine/threonine-protein kinase ppk25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.80">
        <property type="entry name" value="Kinase associated domain 1, KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028375">
        <property type="entry name" value="KA1/Ssp2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001772">
        <property type="entry name" value="KA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF82">
        <property type="entry name" value="KP78A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02149">
        <property type="entry name" value="KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103243">
        <property type="entry name" value="KA1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50032">
        <property type="entry name" value="KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 11 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="15"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="KA1" evidence="14">
        <location>
            <begin position="442"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAI7739725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="490" mass="55516" checksum="6A65A718CF8C58E3" modified="2024-05-29" version="1">MDDNGSGSLPPLSNYRLGKTLGFGAFGKVKVAQHVLTGIKVAIKILDRQSINDSDGERVRREIKIMRLLSHPHIVRLYEVIETRSTIYVVMEYMNSGELFDYITENRRLEENHARHFFQQIISGVESCHLHMVVHRDLKPENLLLDSKGNVKVADFGLANVMRDGHLLKTSCGSPNYASPEVISGHLYAGPEVDVWSCGVILYALLSGGLPFDDEIMTVLLAKIKGGVFTCPRYFSHGARDLIRRMLNVDPVNRITIPEIYKHPWFQINLPRYIGDHSINTSWSSRKVDVRVLEEMDILGFNVQEVIGSLNNLWQNQATVSYSILLHRRLNNLSRQEDNHLGSLELEGTDLRDIYVRPTFPVQGKWVLGFKSCSSAHETMRDVLTVFKRLNVQWKKIGPYNVKCLWKPAAQRCSLPMGINGITLREDTNGVNAPLNTSNNETSSFCDDVKFEIQLYKGSAETYVLDWQRVYGAPFLFIEVCAAFRACVIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CHV5</accession>
    <name>A0AAD5CHV5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MAGE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_030079</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008083">
        <property type="protein sequence ID" value="KAI7742073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CHV5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1200">
        <property type="entry name" value="MAGE homology domain, winged helix WH1 motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1210">
        <property type="entry name" value="MAGE homology domain, winged helix WH2 motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037445">
        <property type="entry name" value="MAGE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041898">
        <property type="entry name" value="MAGE_WH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041899">
        <property type="entry name" value="MAGE_WH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002190">
        <property type="entry name" value="MHD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11736">
        <property type="entry name" value="MELANOMA-ASSOCIATED ANTIGEN MAGE ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11736:SF14">
        <property type="entry name" value="NSE3 HOMOLOG, SMC5-SMC6 COMPLEX COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01454">
        <property type="entry name" value="MAGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01373">
        <property type="entry name" value="MAGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50838">
        <property type="entry name" value="MAGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MAGE" evidence="1">
        <location>
            <begin position="18"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7742073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="234" mass="26111" checksum="778062F9DF309FE6" modified="2024-05-29" version="1">MDTAEPNFSQFGISEEEGEKLVAEVIRYILFKTHQNSGCPIKRDELTQLITKNYHNRSLPALVLSKATSKLSTVFGYDMKELQRARPSSTNQARSSQSSADSKSYIITSQLDSGVYRKHVDDPRKSHLTGLTFVVLGIVNLAGGKITEESLWHHLARLGLSQDDEKNTEFGSTKQALETLVQQRYLQKDKSNGPEGLTLYYELAERGLQATASSSFKESITQMVMSEATTIELD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CI92</accession>
    <name>A0AAD5CI92_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ATPase AAA-type core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_029240</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008208">
        <property type="protein sequence ID" value="KAI7741335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045037">
        <property type="term" value="P:protein import into chloroplast stroma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23076:SF56">
        <property type="entry name" value="INACTIVE ATP-DEPENDENT ZINC METALLOPROTEASE FTSHI 2, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23076">
        <property type="entry name" value="METALLOPROTEASE M41 FTSH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041953002" description="ATPase AAA-type core domain-containing protein" evidence="4">
        <location>
            <begin position="19"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="234"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="ATPase AAA-type core" evidence="5">
        <location>
            <begin position="380"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="278"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="183"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7741335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="449" mass="51345" checksum="FE08F34529DA5551" modified="2024-05-29" version="1">MSVTLTIISATLPQPVLAAKVTQKKRPVKKTESLTPQEWKTWSQGLPVVGDRIAYTDIIDLKREGKLKHVIKPSGKGLRERAVVVLAVLDXXXXVESDGRFWEMWDELGIDSVCVQAYTPPVKTPDVPTPFLGFLSRFPMWMYSFVKPKPVSKKVLELRRQREELKKSRMSEMLSAKKEMVQMEKAARAQRKMEERKRKMEEQKLNSERAMLAASDRYYQMSNTWKQLARDSNVTLAIGMMFFFVFYQTVVLSYKKQKKDYEDRLKIEKAEAEEKKKMRELERGMAGLDDDDDDDAEGKSGEENPYMKMAKQFMKSGARVRRAQNSKNTKLSQYMERGVDVKFSDVAGLGKIRLELEEIVRFFTHGEVYRRRGVKIPGGILLCGPPGVGKTLLAKAVAGEAGVNFFSISASQFVEIYVGVGASRVRALYQEARDSVRFFFIYFSVFTCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CJM9</accession>
    <name>A0AAD5CJM9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Vesicle-associated membrane protein 726</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_010650</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in the targeting and/or fusion of transport vesicles to their target membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Endomembrane system</location>
            <topology evidence="8">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the synaptobrevin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007883">
        <property type="protein sequence ID" value="KAI7742837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CJM9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14824">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15843">
        <property type="entry name" value="R-SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000004">
        <property type="entry name" value="Vesicle-associated membrane protein 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.50:FF:000009">
        <property type="entry name" value="Vesicle-associated membrane protein 724"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.50">
        <property type="entry name" value="Longin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010908">
        <property type="entry name" value="Longin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001388">
        <property type="entry name" value="Synaptobrevin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051097">
        <property type="entry name" value="Synaptobrevin-like_transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042855">
        <property type="entry name" value="V_SNARE_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21136">
        <property type="entry name" value="SNARE PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21136:SF176">
        <property type="entry name" value="VESICLE-ASSOCIATED MEMBRANE PROTEIN 721"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13774">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00957">
        <property type="entry name" value="Synaptobrevin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00219">
        <property type="entry name" value="SYNAPTOBREVN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01270">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50859">
        <property type="entry name" value="LONGIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00417">
        <property type="entry name" value="SYNAPTOBREVIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50892">
        <property type="entry name" value="V_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="195"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="Longin" evidence="11">
        <location>
            <begin position="11"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="V-SNARE coiled-coil homology" evidence="12">
        <location>
            <begin position="131"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7742837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="221" mass="24681" checksum="E4679159C61816C8" modified="2024-05-29" version="1">MAPQGSLIYSLVARGTVVLAEYTEFSGNFATLAVQCLQKLPSTTNRFTYNCDGHTFNYLVENGYAYCVVAVESAGRELPIVFLERIKDDFIKRYGAGKAKTAGAKGLNKEFGPKLKEHMRYCVSHPEEISKIAKVKAQVSEVKGVMMENIEKVLERGEKIDLLVDKTDNLRNQAQDFKKQGTKIKKKMWVENMKVKLIVFAIVAALALVVILSVCPGFKCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CK30</accession>
    <name>A0AAD5CK30_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phospholipase/carboxylesterase/thioesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_030711</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007749">
        <property type="protein sequence ID" value="KAI7743297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CK30"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003140">
        <property type="entry name" value="PLipase/COase/thioEstase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46234">
        <property type="entry name" value="ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02230">
        <property type="entry name" value="Abhydrolase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phospholipase/carboxylesterase/thioesterase" evidence="1">
        <location>
            <begin position="25"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7743297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="253" mass="27573" checksum="1286C7FDB975BE28" modified="2024-05-29" version="1">MSYNSSNRDSGSRNVRRNVHDFGRTFVVMPKETHTATIVWLHGIGEKGSSWTQILETFPLPNVKWICPSAPSRLLSLYGGYPCTAWFNITSMSEDACDDLEGLDASATHVANLLISEPDDVKLGVAGFSMGASVALYSAACRAIGQYGNGNRYPINLSAAVSLSGWLPCSRNLRNRVGASQEAVRRALSLPVLFCHGQVDDVVEYKLGEKSAQTMYSAGFQNLTFQTYNGLGHYTILEEINDVCCWLTACLGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CKB6</accession>
    <name>A0AAD5CKB6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">M8C21_011327</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">AA19_3_7</strain>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010007733">
        <property type="protein sequence ID" value="KAI7743476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CKB6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000004">
        <property type="entry name" value="Calcium-dependent protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000050">
        <property type="entry name" value="Calcium-dependent protein kinase 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01697">
        <property type="entry name" value="PARVALBUMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="56"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="17">
        <location>
            <begin position="236"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="17">
        <location>
            <begin position="321"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="17">
        <location>
            <begin position="359"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="18">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="18">
        <location>
            <position position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAI7743476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="411" mass="46815" checksum="BE09B7FD879BC450" modified="2024-05-29" version="1" fragment="single">NLKILSLKLWEIAVSHLKINKPNPYANAYRPKPESGSGFKLYVLKNPTGHDITQRYNLGRELGRGEFGVTYLCTDSETGERFACKSILKKKLRTSVDIEDVQREVEIMKHLPKHPNIVTIKDTYEDNNAVHIVMELCEGGELFDRIVARGHYTERAAAAIMRTIVEVNFRFTPNLIVTNATAYHIVITEHPWLFDAKAAPNVSLGEVVRSRLKQFSVMNKLKKQALIVIAEHLSVDEVSGIKEMFDMMDTSKRGKINLEELRIGLQKLGQLVNDADLQVLMESVSFVIIFTLLGSRVDLDGDGTLNYNEFVAVNVHLKRMANDEHLHKAFGFFDRNRSGYIEVDELRYVLSDDEENIDGIEEVIHAIMHDVDTDKDGRISYEEFAAMMKAGTDWRKASRQYSRERLNSLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CKY8</accession>
    <name>A0AAD5CKY8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Membralin-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_000756</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007713">
        <property type="protein sequence ID" value="KAI7743557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904294">
        <property type="term" value="P:positive regulation of ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034976">
        <property type="term" value="P:response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019144">
        <property type="entry name" value="Membralin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21650:SF4">
        <property type="entry name" value="MEMBRALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21650">
        <property type="entry name" value="MEMBRALIN/KINETOCHORE PROTEIN NUF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09746">
        <property type="entry name" value="Membralin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="315"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="362"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="391"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="420"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="443"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="139"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="514"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="153"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="514"/>
            <end position="535"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7743557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="581" mass="67075" checksum="EEB87ACA610F8BEC" modified="2024-05-29" version="1">MDPEQTFIRVQERFSHVLTPRIRASLEYIYLIIAISLFCVLVVMHANYVQQPGCSSELTGIATTEAQLIQIKITSAGLWSQSEAPQYTKNNEIDKESVANDEKDDAQIWSNWLISSSGWSGKSALRKLWRSDNEVLEPQPQICTSSSDNIEDAYSKKTKKDESRGRSNKESLKAAVFRKWHGRFSFIWRHTKRILTSIWGYFLLPERARSQHNIRTVNISISARDSCFGNSLVVGLQVFGSLFDKVINXXXXRWQQLLINRLVGYDTILMNSLLNSPGQGYLFNYQTSEFYNLTYAHEKPERSANFGDYFATKCGVLMMSLFVFFTTTMSVSFTLRETQTRMLKFTVQLQHHARHRLPTFQLIFVHVIESLVFVPIMIGILFFLFEFYDDQLLAFMVLILVWLCELFTLISVRTPISMKFFPRFFLLYFLVFHIYFFSYTHGFSYLALSTTSAFMLHLILYFWNHFEVPALQRFMQNRRSQFQQQPDFHITSSTILASTLHITRLNPLRNAINNATNPGPTQPQAENDNMNMNRVTDNEGGGAMNSFSSTLLWILGGASSEGLNSFFSMFRDQGQENQAIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CL81</accession>
    <name>A0AAD5CL81_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">lipid-A-disaccharide synthase</fullName>
            <ecNumber evidence="1">2.4.1.182</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_030373</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a lipid X + a UDP-2-N,3-O-bis[(3R)-3-hydroxyacyl]-alpha-D-glucosamine = a lipid A disaccharide + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:67828"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:137748"/>
            <dbReference type="ChEBI" id="CHEBI:176338"/>
            <dbReference type="ChEBI" id="CHEBI:176343"/>
            <dbReference type="EC" id="2.4.1.182"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.182" evidence="1"/>
    <dbReference type="EMBL" id="JAMZMK010007584">
        <property type="protein sequence ID" value="KAI7744123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CL81"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008915">
        <property type="term" value="F:lipid-A-disaccharide synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009245">
        <property type="term" value="P:lipid A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003835">
        <property type="entry name" value="Glyco_trans_19"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00215">
        <property type="entry name" value="lpxB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30372">
        <property type="entry name" value="LIPID-A-DISACCHARIDE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30372:SF4">
        <property type="entry name" value="LIPID-A-DISACCHARIDE SYNTHASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02684">
        <property type="entry name" value="LpxB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3" id="KW-0441">Lipid A biosynthesis</keyword>
    <keyword evidence="2" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="456"/>
            <end position="490"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7744123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="490" mass="54002" checksum="E8CE0ADB51F35C8D" modified="2024-05-29" version="1">MLLKRMIQNSNRRRPLFFLPSIMRKYFSTSSQAAVDLASKHGEMRIFLVAGEVSGDAIGSRLIAALKNLSPYPIHVAGVGGPMMAKQGLKSLFPMEDIAVMGIWELLPHLKKLQVRMKETVEAAISFRPHAVVTIDSKGFSFRLLKQLKARCCEQGYNAPLHFHYVAPSFWAWKGGEERLNGLSEFVDHVLCILPFEAEVCISHGVPATFVGHPVLEDIQELNLVQVTTEKGWKAQRNADLFRSEYGISSGSTIFTLLPGSRLQEVTRMLPIYSKTINLLKDSVRDPTVVIHVAPNQHVENYIKKAVNEWSVPIVLVPGGSAHTKYDAFSASTAALCTSGTAALELHLARLPCVVAYRAHLLTEWVIRYKAKIPYISLPNILLNSPVIPEALFRACTPTRLASLLMQLLSPPPARAGGSDLYCKESNFDNSTNIEAAAPPACDPTDPNCVITPSRKKPRIVEGSNEDSYAERIKSQTASDDTPEIVVVGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CLZ2</accession>
    <name>A0AAD5CLZ2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">M8C21_020566</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">AA19_3_7</strain>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007773">
        <property type="protein sequence ID" value="KAI7743210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CLZ2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048827">
        <property type="term" value="P:phyllome development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090567">
        <property type="term" value="P:reproductive shoot system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010103">
        <property type="term" value="P:stomatal complex morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000290">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000288">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000107">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000219">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27000">
        <property type="entry name" value="LEUCINE-RICH REPEAT RECEPTOR-LIKE PROTEIN KINASE FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27000:SF444">
        <property type="entry name" value="LRR RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE GSO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="463"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="530"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="559"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="17">
        <location>
            <position position="762"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAI7743210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="762" mass="84405" checksum="E7B103A473A2B179" modified="2024-05-29" version="1" fragment="single">CTLSFVALIFTVYTLVTFNDCIFPFVYRILKSNQLIGPIPSTLSQIPNLKILDLAQNQLSGEIPRLIYWNEVLQYLGLRSNNLVGELSPDICQLTGLWYFDVRNNSLSGTIPSNIGNCTGFQVLDLSYNRLTGEIPFNIGFLQVATLSLQGNQLSGEIPSVIGLMQALAVLDLSCNNLSGQIPPIIGNLTYTEKLYLHNNKLTGSIPPELGNMSKLHYLELNDNQLTGHIPPELGKLTDLFDLNVAGNKLEGHIPDNLSSCTNLNSLNVHGNKLKGTIPAQLQRLESMTYLNLSSNNLAGPIPIEISRIGNLDTLDLSNNKISGPIPSPLGDLEHLLKLNLSRNEFTGYLPAEFGNLRSVMDIDLSYNHLSGVLPQELGQLQNLFTLKLESNNFTGGLTSLVNCLSLSVLNVSYNNLIGDIPTGNNFSKFSPDSFLANPGLCSSSLSSCHASHSTERVTIPRAAILGIALGALILLLMILVAVCRPNNSKPFMDGSYDKPVHYSSPKLVILHMNLALHVYDDIMRMTENLSEKYIIGYGASSTVYKCVLKNCRPVAIKKLYTHYPEYLKEFETELETVGSIKHRNLVSLQGYSLSPSGNLLFYDYMENGSLWDLLHGPLKKKKLDWDTRIQIALGAAQGLAYLHHDCSPRIIHRDVKSSNILLDKDFEAHLTDFGIAKSLCTSKTFTSTYIMGTIGYIDPEYARTSRLTEKSDVYSYGVVLLELLTGRKAVDNESNLHHLQATRSWKPLIRKYLQHARTSAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CM58</accession>
    <name>A0AAD5CM58_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_004998</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007528">
        <property type="protein sequence ID" value="KAI7744481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CM58"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7744481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="117" mass="13748" checksum="C02DBE3B5F5B3CD7" modified="2024-05-29" version="1">MTFAAKRGAYLHFFHYIGKLFWFQAITQTEVYQSESWELSVLAGLFWVLLLFYTTRIECKHGKKVPATPFYVLLLPWVSEKQGLRLFFLFFFCSRKASHKDAAILDSQYTLLAVAFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CQ06</accession>
    <name>A0AAD5CQ06_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">COBRA C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_032057</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the COBRA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007029">
        <property type="protein sequence ID" value="KAI7746148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CQ06"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010215">
        <property type="term" value="P:cellulose microfibril organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052324">
        <property type="term" value="P:plant-type cell wall cellulose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056900">
        <property type="entry name" value="COB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006918">
        <property type="entry name" value="COBRA_pln"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31673">
        <property type="entry name" value="PROTEIN COBRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31673:SF61">
        <property type="entry name" value="PROTEIN COBRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25079">
        <property type="entry name" value="COB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04833">
        <property type="entry name" value="COBRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038122">
        <property type="entry name" value="COBRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="COBRA C-terminal" evidence="7">
        <location>
            <begin position="220"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF25079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7746148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="363" mass="41102" checksum="E7B4AA63F528FA95" modified="2024-05-29" version="1" fragment="single">MLIDDVAISVEKFCVFLDGLFIEPSSLFLAEAYDALDPNGNITIKWDIISWTPDGYVAVVTMFNFQQYRHISAPGWTLGWTWAKKEVIWSMMGGQATDQGDCSRYKTSPPHCCKKTPSIVDLLPGTPYNQQIANCCKGGVINSWAQEPNNYASSFQLSVGAAGTSNKTVRPPKNFTLLAPGPGYTCGPAVVGKPSKFISADGRRVTQAMSEYSFLKLIVEMCTRHMCPIRVHWHVKLNYKDYWRVKVTITNFNYRMNYSQWNLVVQHPNFDNLTQIFSFNYKPLTPYSSINDTAMLWGVKFYNDFLNQAGPMGNVQSELLFRKDKSTFTFEKGWAFPRRVYFNGDNCVMPPPDAYPHLPNASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CR46</accession>
    <name>A0AAD5CR46_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_022202</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007154">
        <property type="protein sequence ID" value="KAI7745725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CR46"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7745725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="69" mass="7582" checksum="B50D536ED3886D40" modified="2024-05-29" version="1" fragment="single">MRWNLSGEPCSGAAVDSTDIDSLEYNPGIKCDCSFPNSTCHITRLKVYAMDAEGPIPEGLWTLVYLTNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CS08</accession>
    <name>A0AAD5CS08_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Nucleobase-ascorbate transporter 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_029598</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006896">
        <property type="protein sequence ID" value="KAI7746774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006043">
        <property type="entry name" value="NCS2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF037981">
        <property type="entry name" value="NCS2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11119">
        <property type="entry name" value="XANTHINE-URACIL / VITAMIN C PERMEASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00860">
        <property type="entry name" value="Xan_ur_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="42"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="100"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="166"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="190"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="228"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="330"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="372"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="397"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="428"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="467"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7746774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="533" mass="58389" checksum="B3061556B745B641" modified="2024-05-29" version="1">MAEAPKAEDITHLPTDQLQGLEYCIDSNPSWGEAIALGFQHYILSLGTAVMIPSFLVPLMGGTSVSLFXXXXGDKVRVVQTLLFVQGINTFLQTLFGTRLPVVIGGSWAFMVPILSIIHDPSLAAIGDDHMRFLATMRAIQGALIVASSVQIILGYSQLWAICSRFFSPVGMVPVIALVGFGLFDRGFPVVGRCVEFGIPMLILFIAFSQYLKQFQPKHMPILERFALLLTIALIWAYAHLLTVSGAYKHHPEQTQMHCRTDKADLISAAPWIKIPYPLQWGAPTFEAGQAFGMMAAVLVSLVESTGAYQAAARLASATPPPAHVLSRGIGWQGIGILLSGLFGTATGSTVSVENVGMMGSTRVGSRRVIQISAGFMIFFSILGKFGALFASIPFPIFAAVYCVLFGFVASVGLSFLQFTNMNSMRNLFIVGVSFFLGLSIPEYFREYTAGALHGPSHTKAEWFNDFLNTVFFSSPTVALMVSVFLDNTLELKDSAKDRGMPWWARFRAFKGDSRNEEFYTLPFNLNRFFPPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CUW9</accession>
    <name>A0AAD5CUW9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Condensin complex subunit 1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_020216</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006473">
        <property type="protein sequence ID" value="KAI7748748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CUW9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000779">
        <property type="term" value="C:condensed chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000796">
        <property type="term" value="C:condensin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010032">
        <property type="term" value="P:meiotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007076">
        <property type="term" value="P:mitotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026971">
        <property type="entry name" value="CND1/NCAPD3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032682">
        <property type="entry name" value="Cnd1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14222">
        <property type="entry name" value="CONDENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14222:SF1">
        <property type="entry name" value="CONDENSIN-2 COMPLEX SUBUNIT D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12717">
        <property type="entry name" value="Cnd1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0226">DNA condensation</keyword>
    <keyword evidence="3" id="KW-0498">Mitosis</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Condensin complex subunit 1 C-terminal" evidence="9">
        <location>
            <begin position="694"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="569"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1044"/>
            <end position="1074"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="958"/>
            <end position="985"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="571"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF12717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7748748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="1090" mass="120422" checksum="47A3B75B2E8A81F7" modified="2024-05-29" version="1" fragment="single">FPDCLKGLVQTMGAIPAIALEACSGSGLGSFNRLCDLCGKILSELLKGEEHGDKGVTAGEVMKALSCSIILSKSQARTFGLGYVVNKMGVMARESDEIRKAMVNFPKYLIGKAPERAELRALAVESILEVVGCLEYEDQIGFSDYVVNMTKGKPQFRLLAVDLILNLIMSLDNPLGHESSWGVKCLRALLERCSDSTAGIRARAITNLAHLVGFLSGNNENKMELMKIMGFGEDGGLNDLLRKRCVDEKAAVRKSALVLISKLTALQGEGGGFDDVLLKIMGVACSDQLVSIRKAAVSALSETFRKFDDENVTKEWLHSVPRLITDNETSIQEECENLFLELVLDRISRAGYNVDDLSLSDGVLSLLKEICNGEVSPWVKKICTNLGKKKKLKPKVAVSLQNIIKKSESMWLTDSKPIEKWLAPPGAWFLLSEVSGFLLKAVDWEFLYHHWQLLDKHESSNAPLEEEDIESNSVDWAGDRVFLLQTISNVSVELPAEAAADLAHNLLKRIEDFSMHSMEVDAHVKALRTLCKRKAKDPQEADSLVIKWVHQLLSKASKTIQAYMTKDSENNKNNSLFTPPSGTRKGNRSSTTMSKLLAQATTAVYTIGSLVLVCPSADLKTITPILHNIITSGVSDPKNKKSPEVLVSFKQTAPSLYIQAWVAMGKICLADGKLAKQYIPLFVQEMEKSDIASLRNNIVVMMTDLCVRYTALVDCYIPNITKCLRDPCELVRRQTFTLLCRLLQRDYVKWRGVVFLRFLLSLVDESDKIRQLADFLFGNILKVKAPLLAYNSFVEAIFVLNDCNAHTGQSNPLSSQKESRLFSIRGNDEKSRSQRMHIYVSLLKQMAPEHLLATFAKVCAEILASASDGMLKIDDVTSQSVLQDAFQVLSCKEIKIPTNKGSSTESSEMEEDVAENAIKGRVITQVVKKSLIQNTIPIFIELKRLLESKNSPLTGSLMECLRILLKDYKNEIEEMLVADKQLQKELIYDMQKYEAQKARSTAAEAVDKGVNVNMHTCSKVSSAVAGALAEATAKSVLRDVNQGAGTPSLSAMNVPKLKSSTGTAAKHKGRSSAVLESLRRRQSFDLDDDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CXF6</accession>
    <name>A0AAD5CXF6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">PRA1 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_021234</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 7">May be involved in both secretory and endocytic intracellular trafficking in the endosomal/prevacuolar compartments.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 7">Membrane</location>
            <topology evidence="2 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the PRA1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006235">
        <property type="protein sequence ID" value="KAI7750016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CXF6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004895">
        <property type="entry name" value="Prenylated_rab_accept_PRA1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12859:SF0">
        <property type="entry name" value="PRA1 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12859">
        <property type="entry name" value="PRA1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03208">
        <property type="entry name" value="PRA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="52"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="77"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="165"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7750016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="218" mass="24817" checksum="56A89F1429253D5D" modified="2024-05-29" version="1">MDWATVSTEDLIDALKEVDWSSPPRPINEFFNKFTFPRSSNKWNSRLKCNLYYYRTNYFLMILFILGLGFLRRPLAIVAAVSTALTIAFLNDSFAGTFSEKLTRFVRQFSPHLAAKMRPPPMPVLRGRPSSKRAIYICGRPRWQFVIMSSAVSVFLWFVSCGLLILLWALTVGLLATIMHASFRTPNLKARLNTFREEFRASCEVAKWEIGSKGAGSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CYI1</accession>
    <name>A0AAD5CYI1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">WAT1-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_015419</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the drug/metabolite transporter (DMT) superfamily. Plant drug/metabolite exporter (P-DME) (TC 2.A.7.4) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006181">
        <property type="protein sequence ID" value="KAI7750242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CYI1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030184">
        <property type="entry name" value="WAT1-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31218">
        <property type="entry name" value="WAT1-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="30"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="66"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="96"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="129"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="180"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="7">
        <location>
            <begin position="5"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7750242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="206" mass="22359" checksum="314A2075B58BF674" modified="2024-05-29" version="1">MEKNKAYFVVILIQLIYTGMFLLSKAAFDVGMSTCIFVFYRQAAGTLILVPFAFITEGKDSSPLTFMTFCKIFGLSLLGITTSLNIHGIALKYTSASLAAAASNCLPAITFFMACLMRFESIKLRTSSGIAKFTGIVICMGGAATMAFFKGPELRLLLHHHLLPSHAQLNVAEGSGSGSIWVKGVFLMLLSVSCWGVWIVLQVYNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CYR4</accession>
    <name>A0AAD5CYR4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_008214</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006078">
        <property type="protein sequence ID" value="KAI7750833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CYR4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7750833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="144" mass="15818" checksum="0E35EFD4E0519F7C" modified="2024-05-29" version="1">MATSSSFSSTAAATATATDVLDSTQSLLKLMRGHEVSIAELSALSSSRTVYQKNGNIFFRTSVQQATASEKSKIVSNSLCSNLIRSKQSYRNRISLNETRPAHLFCICEATGFYSKCNCHNPTNSGHRGYEFIPSEVDNHQRSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CZR9</accession>
    <name>A0AAD5CZR9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_013024</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006093">
        <property type="protein sequence ID" value="KAI7750722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CZR9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46699">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE STN8, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46699:SF1">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE STN8, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="137"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7750722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="499" mass="56065" checksum="B7302F555AEBB40C" modified="2024-05-29" version="1">MAVATTTTTITMLSSTNLKLYSNSISFSPIIKPTHFKRHPSRLNVSFNQLSDTLLENTLHLDQYPVFKTGYDQFQQVTNDFPEVEKWGVFVFAGLLWVYLTARPGVLIGAIDAYLLGPVQKVVDGLTGRRSLKRNDFLIGDKLGEGSFGVVYSGVVVPKNMTVEERVRNSGSRRKQLEKDPRFKEKVILKQVKVGVQGAIECGDFEEWFNYRLSRAAPDTCAEFLGSFISDKTNSIFTKGEKWLVWKFEGDRDLGDYMKDRSFPLNLESLMFGRVLQGLESIERNALIIKQIMRQIISSLKKIHDTGIVHRDVKPANLVVTKKGRIKLIDFGAATDLRIGKNYVPNKGLLDPDYCPPELYVLPEETPSPPPEPVAALLSPILWQLNSPDLFDMYSAGIVLMQMAIPSLRSSAGLKNFNLELKAVNYDLKRWREKTRTRPDTSVLDLDSGRGWDLATKLISERGSLRRGRLSASDALRHPYFLLGGDQAASVLSKLSLSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D047</accession>
    <name>A0AAD5D047_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">E3 ubiquitin-protein ligase PRT1-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_014806</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006113">
        <property type="protein sequence ID" value="KAI7750622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D047"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000489">
        <property type="entry name" value="E3 ubiquitin-protein ligase PRT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.90:FF:000014">
        <property type="entry name" value="E3 ubiquitin-protein ligase PRT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000433">
        <property type="entry name" value="Znf_ZZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043145">
        <property type="entry name" value="Znf_ZZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15898">
        <property type="entry name" value="BIFUNCTIONAL APOPTOSIS REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15898:SF19">
        <property type="entry name" value="ZINC FINGER, ZZ-TYPE, ZINC FINGER, RING_FYVE_PHD-TYPE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13923">
        <property type="entry name" value="zf-C3HC4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00569">
        <property type="entry name" value="ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00291">
        <property type="entry name" value="ZnF_ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50135">
        <property type="entry name" value="ZF_ZZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="17"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="156"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="ZZ-type" evidence="7">
        <location>
            <begin position="270"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="371"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="393"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7750622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="402" mass="44708" checksum="30ADDC2DAEB0FF0B" modified="2024-05-29" version="1">MEEEEGQSEQVSDHFMCAVCLDLLYKPVVLVCGHVTCLWCCYQSMDKKGQSHCPLCRYPYRHFPAIFQMLDIFLKKLYPISYGRRKQQSLDDKQQSSLGFALDEELDHLSIVDQPIHFISEDNVAVVSSSSTDENKSNAGNSGRNCKQVTVADVLCSACKQLLFQPVVLNCGHAYCEACVTIPEDGVIKCQSCQCRHPSGFPKVCKELDYVLEEQFSSEYALRKSSAQLSQEQFQKRNLLEEANEQGSKFSYPPGENFLQWWAIHGSKYHPGVGCDMCGMCPIIGYRYQCKDCKEKNSYDLCGDCYKIGTKVPGRFNQKHTSQHRLELVKAVINQDVIYRLLSGQLEVLSASRNRTLASASYPLDVDVEIGGGGGDGGGDGDDGPTESAIDSRGQSSNETAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D0C5</accession>
    <name>A0AAD5D0C5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Pectinesterase inhibitor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_013044</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>[(1-&gt;4)-alpha-D-galacturonosyl methyl ester](n) + n H2O = [(1-&gt;4)-alpha-D-galacturonosyl](n) + n methanol + n H(+)</text>
            <dbReference type="Rhea" id="RHEA:22380"/>
            <dbReference type="Rhea" id="RHEA-COMP:14570"/>
            <dbReference type="Rhea" id="RHEA-COMP:14573"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17790"/>
            <dbReference type="ChEBI" id="CHEBI:140522"/>
            <dbReference type="ChEBI" id="CHEBI:140523"/>
            <dbReference type="EC" id="3.1.1.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the pectinesterase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the PMEI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006093">
        <property type="protein sequence ID" value="KAI7750742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D0C5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004857">
        <property type="term" value="F:enzyme inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030599">
        <property type="term" value="F:pectinesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042545">
        <property type="term" value="P:cell wall modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15798">
        <property type="entry name" value="PMEI-like_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.40">
        <property type="entry name" value="Invertase/pectin methylesterase inhibitor family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035513">
        <property type="entry name" value="Invertase/methylesterase_inhib"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000070">
        <property type="entry name" value="Pectinesterase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006501">
        <property type="entry name" value="Pectinesterase_inhib_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01614">
        <property type="entry name" value="PME_inhib"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31707">
        <property type="entry name" value="PECTINESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01095">
        <property type="entry name" value="Pectinesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04043">
        <property type="entry name" value="PMEI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00856">
        <property type="entry name" value="PMEI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101148">
        <property type="entry name" value="Plant invertase/pectin methylesterase inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0063">Aspartyl esterase</keyword>
    <keyword evidence="6" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pectinesterase inhibitor" evidence="9">
        <location>
            <begin position="38"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="241"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="258"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7750742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="567" mass="63476" checksum="C4F283F014455217" modified="2024-05-29" version="1">MAKELIASVLSLGLSIAFILVIVALVAKDANLEDIHLMNTKALKTMCKPTEYKEACYYGLTEVAKNTTATKKDYLFATFHSTIVELQKAAKKCNSKRNDLNGRTDEYAEHARDDLKNCEKLLGFGIDDLQHVLKVAHKTKTKTIPNQADQILVWLTAVRAYQTTCVNEIKDEKLQKEIAKMLEKANKHTYNSQKIIYNVAKILNDFGMDLSGDKGASGRRRLLEEGQVIIEQHGFPSWVPFGDRRLLGDDESNEDGGDDKRDEPKPKPKPDDDEDDKPHEPKLTGQEYFRSIEPGPMDKMIKPNVIVAQDGSGQFKTIKEALAADPLDHKGRYIIYIKSGVYNEGQIVVNRQQHNVYMYGDGCGKTIITGQLSNRITNIATSNTATFVAEGERFMARNIGFRNTIGPIGGQAVAFRSQSPHTVMVECSFEGYQSTLYYHTHDQFYKNCVVSGTKDFVFGRGRAFFQDTKFVIRNPENGQTNTIIADGRMKYEEAAGVVFQNCQIMAAPELAKATTKSYLGRPWAPRVAWSGFEVFEDEEEAAKYCADKFIDAGSWLPKAGVPVNLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D236</accession>
    <name>A0AAD5D236_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_019392</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005673">
        <property type="protein sequence ID" value="KAI7752463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D236"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF237">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7752463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="111" mass="12504" checksum="6A3284246B18470F" modified="2024-05-29" version="1">MAPEVLRNEPSDEKSDVYSFGVVLWELCTMQQPWNGMNPMQVVGAVGFQHRRLEIPNDMDPVIADIITKCWQTDPKVRPTFADIMAALKPLQKLNASSKTVKRVDQSDSTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D279</accession>
    <name>A0AAD5D279_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Chalcone synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_019795</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the thiolase-like superfamily. Chalcone/stilbene synthases family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005834">
        <property type="protein sequence ID" value="KAI7751725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D279"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030639">
        <property type="term" value="P:polyketide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00831">
        <property type="entry name" value="CHS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000014">
        <property type="entry name" value="Chalcone synthase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012328">
        <property type="entry name" value="Chalcone/stilbene_synt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001099">
        <property type="entry name" value="Chalcone/stilbene_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011141">
        <property type="entry name" value="Polyketide_synthase_type-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11877">
        <property type="entry name" value="HYDROXYMETHYLGLUTARYL-COA SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11877:SF10">
        <property type="entry name" value="TYPE III POLYKETIDE SYNTHASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02797">
        <property type="entry name" value="Chal_sti_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00195">
        <property type="entry name" value="Chal_sti_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Chalcone/stilbene synthase N-terminal" evidence="3">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="Chalcone/stilbene synthase C-terminal" evidence="4">
        <location>
            <begin position="80"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7751725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="236" mass="25722" checksum="EB399E5EC3E55261" modified="2024-05-29" version="1">MLYFSGCSGGVAGLRVAKDIAENNPGSRVLLATSETTIIGYKPPSVKRPYDLVGVVLFGDGAGAMIIGSEPTRSESPLFELHTAIQNFLPDTEKVIDGRLTEEGISFKLDRELPEIIVDNVKGFCDKLLGQFGLDDKIDIDYNDLFWAVHPGGPAILNRIEKKFDLWPEKLIASRRALADYGNASSNTIVYVLEYVIEESLKRKNEGKDGGPDLGLILAFGSGVTFEGILTRNLTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D2B7</accession>
    <name>A0AAD5D2B7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Desiccation-related protein PCC13-62-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_011790</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005916">
        <property type="protein sequence ID" value="KAI7751414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D2B7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052965">
        <property type="entry name" value="Pigment-catalase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31694">
        <property type="entry name" value="DESICCATION-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31694:SF12">
        <property type="entry name" value="DESICCATION-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13668">
        <property type="entry name" value="Ferritin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7751414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="180" mass="19640" checksum="571B3AE3B97F3D35" modified="2024-05-29" version="1">MASSSFTTTMILTKFLIFLFISTTVNSIFILPKTDVALLEFPLNLEYFEAEYFLFGSMGKGLDTIEPGLAAGGPPPIGAKQANLSSIIKDIITQFGYQEVGHLRAIKKRVRGFPRPLLNLSAESFGTVMNDAFGMPLSPPFDPYANDVNYLLSCYVIPYVGLTGYVGANPKLLSPISKKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D341</accession>
    <name>A0AAD5D341_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Exoribonuclease phosphorolytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_033270</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RNase PH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005690">
        <property type="protein sequence ID" value="KAI7752432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D341"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035925">
        <property type="term" value="F:mRNA 3'-UTR AU-rich region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000467">
        <property type="term" value="P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071028">
        <property type="term" value="P:nuclear mRNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071035">
        <property type="term" value="P:nuclear polyadenylation-dependent rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071038">
        <property type="term" value="P:TRAMP-dependent tRNA surveillance pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034473">
        <property type="term" value="P:U1 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034476">
        <property type="term" value="P:U5 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11368">
        <property type="entry name" value="RNase_PH_RRP45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="entry name" value="GHMP Kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001247">
        <property type="entry name" value="ExoRNase_PH_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036345">
        <property type="entry name" value="ExoRNase_PH_dom2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050590">
        <property type="entry name" value="Exosome_comp_Rrp42_subfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033100">
        <property type="entry name" value="Rrp45"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11097">
        <property type="entry name" value="EXOSOME COMPLEX EXONUCLEASE RIBOSOMAL RNA PROCESSING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11097:SF27">
        <property type="entry name" value="POLYRIBONUCLEOTIDE NUCLEOTIDYLTRANSFERASE, PNPASE_RNASE PH DOMAIN PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01138">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55666">
        <property type="entry name" value="Ribonuclease PH domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="6">
        <location>
            <begin position="37"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="260"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="279"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="307"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7752432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="367" mass="40132" checksum="C5FE43183679D926" modified="2024-05-29" version="1">MEQRLANTLIMTVNEKKFIENALVSDIRVDGRRPFDYRNLTIRFGSEDGSSEVQLGKTQVLAFVTGQLVQPYRDRPNEGSFAIYTEFSPMADPSFEPGRPGDSAIELGRIVDRGLRESRAVDTESLCVIAGKVVWSIRIDLHIIDNGGNLVDAANIAALAALSTFRRPECTVGGEDGQELTIHPPDVREPLPLILHHFPVAVTFGFIGKENIVKAGGDGVLQSVIMQCLRIASVKAGDITSKIKNAVESYNTQRSLRKIKRHNPSSAVSNVAKHHTVREPNSQSVDTNALRASQPSCATPVDKLNEESTQVNPETKSTQQSSDMNSSASTHEVTDIGSPEKQSNTLKDAVKPKHKRRKKSTSNVNAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D3K7</accession>
    <name>A0AAD5D3K7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_006930</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005644">
        <property type="protein sequence ID" value="KAI7752657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D3K7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7752657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="222" mass="26317" checksum="006EF7D2291B96CB" modified="2024-05-29" version="1" fragment="single">NTIQVQSQNKSQRSATRVIRSNLETLWSGPWDSWRDVPTEDRTRLFERFQMYFQWEKKHNASIYRCWENCIAGKIPNLLRGLRNAAINTAIQKHLLEEDEEDIDMSILIDFKPAWIKSEIWKQMLDNWNTPEWKAKSLRNKEIRRKATGGKHTLGYQTYVTAQRKAVKYNGYVVEKYGNERREHPKFDDDLWYRASGGMNKGKVYGLGNVRDSYVHDKEDPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D4E3</accession>
    <name>A0AAD5D4E3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_001167</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005543">
        <property type="protein sequence ID" value="KAI7753107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D4E3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080090">
        <property type="term" value="P:regulation of primary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000218">
        <property type="entry name" value="Myb transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015495">
        <property type="entry name" value="Myb_TF_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47999">
        <property type="entry name" value="TRANSCRIPTION FACTOR MYB8-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47999:SF24">
        <property type="entry name" value="TRANSCRIPTION FACTOR MYB90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="13"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="13"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="66"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="70"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7753107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="259" mass="29718" checksum="6251BC763E7B55AE" modified="2024-05-29" version="1">MATKRMRQSYDTSSVVRKGAWTAEEDTLLKNCIEKFGEGKWHLVPLRAGLNRCRKSCRLRWLNYLRPNIKRGDFGEDEVDLMLRLHKLLGNRWALIAGRIPGRTANDVKNYWNTHLKSRVKKPKEQHNDGEPSKHTMVTIIKPQPRTISKSLKLYSNIQSHATHNLTTSSNIGISQNLYNVSPDVPSPTLHVDKINEYLDELFDDRENEIHDEVGWPFGGFLAEDDGLDVVKHVDTDQNSLFAFPIDDVDMLDLLSIDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DAF0</accession>
    <name>A0AAD5DAF0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_014595</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the plant LTP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000209">
        <property type="protein sequence ID" value="KAI7756978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DAF0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00010">
        <property type="entry name" value="AAI_LTSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.110.10">
        <property type="entry name" value="Plant lipid-transfer and hydrophobic proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036312">
        <property type="entry name" value="Bifun_inhib/LTP/seed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016140">
        <property type="entry name" value="Bifunc_inhib/LTP/seed_store"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043325">
        <property type="entry name" value="LTSS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33044">
        <property type="entry name" value="BIFUNCTIONAL INHIBITOR/LIPID-TRANSFER PROTEIN/SEED STORAGE 2S ALBUMIN SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14368">
        <property type="entry name" value="LTP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00499">
        <property type="entry name" value="AAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47699">
        <property type="entry name" value="Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042198546" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein" evidence="11">
        <location>
            <begin position="28"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="189"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical" evidence="12">
        <location>
            <begin position="65"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="142"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="159"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7756978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="209" mass="21026" checksum="01C4E565A56DB0B9" modified="2024-05-29" version="1">MVRSNTTIMIVGILVGLIFTGLPRLEAQSPMGAPVMSPGIPMASPDSPTVMSPSSPTAMAPGPDCMTALTNVTDCLTFVQEGSNLTVPDKNCCPEFAGLLESNPICLCELIGQAATYSIDLKKAEMLPSVCKVQTPPLSACPGNSPTLAPTPGTASDMGPGSTIAASPGPGGAAASPSESGTNGASSTAVNNLSTIICLAVAIFVAYYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DBR0</accession>
    <name>A0AAD5DBR0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">U-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_006520</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000018">
        <property type="protein sequence ID" value="KAI7758191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DBR0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057649">
        <property type="entry name" value="PUB62-63_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003613">
        <property type="entry name" value="Ubox_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33644">
        <property type="entry name" value="U-BOX DOMAIN-CONTAINING PROTEIN 62-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33644:SF7">
        <property type="entry name" value="ZINC FINGER, RING_FYVE_PHD-TYPE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23112">
        <property type="entry name" value="PUB62-63_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51698">
        <property type="entry name" value="U_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="U-box" evidence="4">
        <location>
            <begin position="159"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="81"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="12"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="92"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7758191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="338" mass="36871" checksum="5DC14FC06BEAD3BC" modified="2024-05-29" version="1">MQNWNSRGSDGSDADGSDDDDDEDDVEGLVNNNNNNISDNKNNNNNRGQGHSSEKDGNVKLNPLSSFGSGREVLKVGSLMSSGSDLNDVKPSSSENHQQESSHYPNAVALVDRDGELYYSQYLHGTEGFGSGLKDTVVDNGCGFSGRKDVSYAGESGDSLRAILSDPLTGALMDDAMILPCGHSFGSGGMQHVMKMKACYTCSRLVTEDSVAPNLALRSAVLAFRREEESQVHHASKRRRERLDQDRINYNDLTDTRGKGLQFPFSVADRVIIKGNKRTPERFVGCEAVVTTQCLNGWYVVKTLDTAESIKVQYRSLAKVSDTSSSHPTSSKMAPNWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DCE5</accession>
    <name>A0AAD5DCE5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">S1 motif domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_004300</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eIF-2-alpha family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010001220">
        <property type="protein sequence ID" value="KAI7755360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DCE5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005850">
        <property type="term" value="C:eukaryotic translation initiation factor 2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04452">
        <property type="entry name" value="S1_IF2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1130:FF:000001">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.190:FF:000003">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000015">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1130">
        <property type="entry name" value="EIF_2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.190">
        <property type="entry name" value="Translation initiation factor 2, subunit 1, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044126">
        <property type="entry name" value="S1_IF2_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024055">
        <property type="entry name" value="TIF2_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024054">
        <property type="entry name" value="TIF2_asu_middle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011488">
        <property type="entry name" value="TIF_2_asu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10602">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10602:SF3">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT ALPHA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07541">
        <property type="entry name" value="EIF_2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110993">
        <property type="entry name" value="eIF-2-alpha, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116742">
        <property type="entry name" value="eIF2alpha middle domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S1 motif" evidence="5">
        <location>
            <begin position="10"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7755360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="357" mass="40050" checksum="6994BD84393B3AA1" modified="2024-05-29" version="1">MYEAPYPEVGTLVMIQVTEIGAVVSHVSLLEYNNIKGRIQLSDRRRTRSVGSVIKVGSIEVAMVLRVDAERGYIDLSRKRLSEQDVQICVDRYSKSKVVHSIMRHVAVTKFVDLEDLYIHVGWPLYRKYGHAFEAFKVIVNDPDSVLKPLTQELTEFAPDGRREVTKVVPAISGDVIEALVRIIKIRMIPHPVKIRADIEMECVHYDGILHIKEAMRKAEAAGTKDCPVKIRLVAPPSYVLDTQTLDKEQGIKVLSDAIAACAATMEHHKGKLTVKEPPRSVSKLEDKRLAELMTKLRLENAEIVALAPLSARVKAFGSLMMVTRGYWEFAGEIWSRSMEEHTLEQGGGGSLTIIVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DEK8</accession>
    <name>A0AAD5DEK8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">3-hydroxyisobutyryl-CoA hydrolase</fullName>
            <shortName evidence="2">HIB-CoA hydrolase</shortName>
            <shortName evidence="2">HIBYL-CoA-H</shortName>
            <ecNumber evidence="2">3.1.2.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">3-hydroxyisobutyryl-coenzyme A hydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_003138</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Hydrolyzes 3-hydroxyisobutyryl-CoA (HIBYL-CoA), a saline catabolite. Has high activity toward isobutyryl-CoA. Could be an isobutyryl-CoA dehydrogenase that functions in valine catabolism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>3-hydroxy-2-methylpropanoyl-CoA + H2O = 3-hydroxy-2-methylpropanoate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20888"/>
            <dbReference type="ChEBI" id="CHEBI:11805"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57340"/>
            <dbReference type="EC" id="3.1.2.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid degradation; L-valine degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.4" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010000037">
        <property type="protein sequence ID" value="KAI7757957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DEK8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003860">
        <property type="term" value="F:3-hydroxyisobutyryl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006574">
        <property type="term" value="P:L-valine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045004">
        <property type="entry name" value="ECH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032259">
        <property type="entry name" value="HIBYL-CoA-H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43176:SF6">
        <property type="entry name" value="3-HYDROXYISOBUTYRYL-COA HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43176">
        <property type="entry name" value="3-HYDROXYISOBUTYRYL-COA HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16113">
        <property type="entry name" value="ECH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl-CoA hydratase/isomerase" evidence="3">
        <location>
            <begin position="14"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU369070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7757957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="129" mass="14187" checksum="E53A697A1BC21802" modified="2024-05-29" version="1" fragment="single">MENHVVLFWSGHWSFGASYYRKEFCLDYLLATYKKPSLVILNGNVMGGGVGISIHSTFRIVTENTVFAMPEVLIGLFPDVGASYFLSRLPGFFGKLILIPGEYIGLTGARLNGVEMLAFGLGTHFISSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DEY5</accession>
    <name>A0AAD5DEY5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_013359</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Plays an important role in the elongation step of protein synthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">P1 and P2 exist as dimers at the large ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic ribosomal protein P1/P2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000355">
        <property type="protein sequence ID" value="KAI7756530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DEY5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002182">
        <property type="term" value="P:cytoplasmic translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05833">
        <property type="entry name" value="Ribosomal_P2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1410:FF:000002">
        <property type="entry name" value="60S acidic ribosomal protein P2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01478">
        <property type="entry name" value="Ribosomal_L12_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038716">
        <property type="entry name" value="P1/P2_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027534">
        <property type="entry name" value="Ribosomal_P1/P2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044076">
        <property type="entry name" value="Ribosomal_P2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21141">
        <property type="entry name" value="60S ACIDIC RIBOSOMAL PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21141:SF5">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN P2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00428">
        <property type="entry name" value="Ribosomal_60s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="86"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="91"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7756530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="114" mass="11382" checksum="ACC20DBCB097972A" modified="2024-05-29" version="1">MKVVAAYLLALLGGNTSPSAEDLKNILGSVGADCDDDKIELLLSEVKGKDITELIASGREKLASVPSGGGGGVAVAAAAGGGAAPAAAAAESKKEEKVEEKEESDDDMGFSLFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DGJ1</accession>
    <name>A0AAD5DGJ1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Thaumatin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_031438</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the thaumatin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000138">
        <property type="protein sequence ID" value="KAI7757310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DGJ1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09218">
        <property type="entry name" value="TLP-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.110.10:FF:000002">
        <property type="entry name" value="Thaumatin-like protein 1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.110.10">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037176">
        <property type="entry name" value="Osmotin/thaumatin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001938">
        <property type="entry name" value="Thaumatin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017949">
        <property type="entry name" value="Thaumatin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31048">
        <property type="entry name" value="OS03G0233200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00314">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002703">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00347">
        <property type="entry name" value="THAUMATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00205">
        <property type="entry name" value="THN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49870">
        <property type="entry name" value="Osmotin, thaumatin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00316">
        <property type="entry name" value="THAUMATIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51367">
        <property type="entry name" value="THAUMATIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042145902" description="Thaumatin-like protein" evidence="4">
        <location>
            <begin position="23"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="31"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="79"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="94"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="146"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="151"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="159"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="180"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="190"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR002703-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7757310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="243" mass="26262" checksum="9CC976F3E1CF0EC3" modified="2024-05-29" version="1">MKTMQLHTIFFSLLLSFHGISATTFIMLNKCTYPVWPGIQPGAGSPVLARGGFKLPPKKSYTLFLPPAWSGRLWGRVGCSFDSFGRGKCATGDCGGSLFCNGAGGTPPATLAEITLTAEQDFYDVSLVDGYNIPMAIKPFKGKGKCNYAGCISDLNVMCPVGLQVRSRDKRRVVACRSACSAFNSPKFCCTGRYGNPQTCKPTAYSRIFKVACPKAYSYAYDDPTSIATCKGASYLLTFCPHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G1R9</accession>
    <name>A0AAD5G1R9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">FAD-binding PCMH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_015477</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oxygen-dependent FAD-linked oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012184">
        <property type="protein sequence ID" value="KAI7724616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G1R9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.462.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.10">
        <property type="entry name" value="Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012951">
        <property type="entry name" value="BBE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016167">
        <property type="entry name" value="FAD-bd_PCMH_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32448">
        <property type="entry name" value="OS08G0158400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08031">
        <property type="entry name" value="BBE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="7">
        <location>
            <begin position="1"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7724616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="430" mass="48710" checksum="8A4F2FD6B7826A5C" modified="2024-05-29" version="1">MRIRSGGHDYEGLSYTADVPFVMLDLVNMRSIDINVENQTAWVQGGALLGELYYTISQKTDTLYFPAGMCPSVGVSGFLSGGGYGNLLRKYGTGADNVLDARFMDVNGNILDRESMGEDLFWAIRGGGASSFGIVLAWKLRLVPVPERVTLFIVSVTLEQGATEIFHKYQYVLPNFNRDLQMRVQINSEYIGNTTNKTIRILFHGIYQGNADTLLPLLNQSFPELNVTREICQEVRMVQSTLQFGGFSTSAHVEVLTNRSAIPKTNFKSKSDYVRTPIPKSGLIKIWEKMFENDMSEMLQMYTFGGKMEDYSDTATPYPHRAGVLYQVYKIVNLIDQPSDTTMISLKRIAWLRSFDETLESYVSSNPREAYLNYNDLDLGFGSGSYEEASVWGERYWKKENFQKLIQIKSIVDPGNFFRHPQSIPSFSYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G260</accession>
    <name>A0AAD5G260_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcription factor MYC/MYB N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_029700</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011975">
        <property type="protein sequence ID" value="KAI7725352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G260"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025610">
        <property type="entry name" value="MYC/MYB_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46633:SF3">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE WNK (WITH NO LYSINE)-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46633">
        <property type="entry name" value="TRANSCRIPTION FACTOR MYC/MYB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14215">
        <property type="entry name" value="bHLH-MYC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription factor MYC/MYB N-terminal" evidence="3">
        <location>
            <begin position="13"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7725352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="203" mass="23153" checksum="B6A13E84A5FB6A1A" modified="2024-05-29" version="1" fragment="single">MEDMQQVAVTHLLQHTLRSFCIHENSQWVYAVFWRILPRNYPPPKWDGVGGAYDRSRGNRRNWILVWEDGFCNFTASSSSSTSGRECSGYGDHEYQQSSHGLQPELFFKMSHEIYNYGEGLIGKVAADQSHKWIFKEPNDQEINFLSAWNNSADSHPRTWEAQFQSGIKTIALIAVREGVIQLGSIHKVASLSLSHTHTEAPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G3D9</accession>
    <name>A0AAD5G3D9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Universal stress protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_002435</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011399">
        <property type="protein sequence ID" value="KAI7727344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G3D9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36081">
        <property type="entry name" value="CELL WALL INTEGRITY/STRESS RESPONSE COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36081:SF1">
        <property type="entry name" value="CELL WALL INTEGRITY_STRESS RESPONSE COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7727344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="214" mass="23304" checksum="8F951F3E5F5B72F0" modified="2024-05-29" version="1">MAFTTCSSLAHVETSSKPSIIITSSFPISPRLSIFLSPSSFSSSSIYQESKKLSFPPNLLKTDHKAAIRPQAAAVDAVAEAFTEFKHLLLPITDRNPYLSEGTRQAAATTAALAKKYGADITVVVIDEKLKESIPEHDTQLSSIRWHLSEGGFQEFKLLERLGEGSKPTAIIGEIADDMNLDLVIMSMEAIHSKHVDANLLAEFIPCPVILLPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G427</accession>
    <name>A0AAD5G427_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_003346</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011497">
        <property type="protein sequence ID" value="KAI7726898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G427"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045735">
        <property type="entry name" value="Spore_III_AA_AAA+_ATPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20953:SF13">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20953">
        <property type="entry name" value="KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19568">
        <property type="entry name" value="Spore_III_AA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="4">
        <location>
            <begin position="172"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="312"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7726898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="384" mass="43082" checksum="5C1AC7CEB6F944D0" modified="2024-05-29" version="1">MSMCYSSTSPSFYKHHIPTLFKPVSISTRTIKPIIRSNCTPPPSSSSDDDDTNDIHQLLKILPHDLRHNLVMESKRDQLLEVILDLGRLPEARYLGDSGRRYLRDTEVSVEELEYAENAIGEIGGDNRAGITGTLHRISAVRNRKGVVVGLTCRVGRAVRGHIDMVRDLLQFGESILFIGRPGVGKTTVMREISRVLSDELQKRVVVVDTSNEIGGGGDIPHPAIGSTRRMQVSKPFMQHKVMIEAVENHMPEVIIIDEISTRNEVNACKSIAEGGVMLIGSAHGDRLENIIKNPVLSHLAGGVETVTLGDEEARKRNSRKSVSQRSGPPTFPFMIEMRDRHYWVVHKTERSVDALLRGEKPRVEVRKRDKQMKVIIEKWKIEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G4E6</accession>
    <name>A0AAD5G4E6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">M8C21_029986</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">AA19_3_7</strain>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010011429">
        <property type="protein sequence ID" value="KAI7727151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G4E6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001023">
        <property type="entry name" value="Os07g0541700 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000178">
        <property type="entry name" value="serine/threonine-protein kinase PBS1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045874">
        <property type="entry name" value="LRK10/LRL21-25-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27009">
        <property type="entry name" value="RUST RESISTANCE KINASE LR10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 15 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 15 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 16" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="6"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="18">
        <location>
            <begin position="61"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAI7727151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="223" mass="25004" checksum="636A98652C3D388D" modified="2024-05-29" version="1">MVAVLGVVSVTILILIILCFKRKTGWRKKAKYNAKVEIFLKNHEFLAPKRFSYAQVEKMTQSFKFKLGQGGFGSVYKGELGNQNLVAVKVLSGLKGDGEDFINEVASVGRTSHVNIVNLIGFCLEGRKSALIYEFMPNGSLERFIYNRGSSNSSQLGWEKLHQIAIGIARGLEYLYRGCSTRILHFDIKPHNILLDNDFCPKISDFGLAKCSGRKKARYQCPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G573</accession>
    <name>A0AAD5G573_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_002821</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011282">
        <property type="protein sequence ID" value="KAI7727976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G573"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7727976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="231" mass="26364" checksum="296AFCB516B0334A" modified="2024-05-29" version="1" fragment="single">MLKELYLDGNPIDSMPDCVRSLSRLERLSFNGCGNLKTVMCARIQLKHLGIYTCQSLEKVTFNPVISGVPLPVHGQKSKTYALTEIQHIFKIQALSEIDEEVLCSLGWINIAYLNHCQFSKIDWWGVYILERMILPAQMVYEHGIFSTYLQGKEVPEWFTQRSSGSSFTLQSPPENGKIKGINFCIVRTISSMKEAGPWRIKIRNLTKNSSWTYTPMMRMVPEEDAFEDGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G5H0</accession>
    <name>A0AAD5G5H0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_006360</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Phosphate:H(+) symporter (TC 2.A.1.9) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011258">
        <property type="protein sequence ID" value="KAI7728188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G5H0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009705">
        <property type="term" value="C:plant-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022821">
        <property type="term" value="F:solute:potassium antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090333">
        <property type="term" value="P:regulation of stomatal closure"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23504">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23504:SF114">
        <property type="entry name" value="PROTEIN ZINC INDUCED FACILITATOR-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="14"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="52"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7728188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="89" mass="9607" checksum="8BBECE71066224AF" modified="2024-05-29" version="1" fragment="single">MIRDFNIAETEEDISYYAGFVGAAYMIGRALTSVFWGMCADRYGRKPVSTSWGIGLIIGPALGGFLAQIFSLWAVSPKSLGGLSYTTVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G6X7</accession>
    <name>A0AAD5G6X7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_022754</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010687">
        <property type="protein sequence ID" value="KAI7730662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G6X7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35106">
        <property type="entry name" value="BNAA07G25190D PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35106:SF5">
        <property type="entry name" value="CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7730662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="140" mass="15825" checksum="B1CF0046DFD6F6B3" modified="2024-05-29" version="1" fragment="single">MATQICSTNSSISSHNTSFILHNPIKLHHSISFSTPIYRLSKYRVHRKHSIQCSLEETKPVTLRTCKQCKTQFDPSLNHPQACRFHTAHFGGETKRKFESVYSGGTMNTPESGKVFQYWHCCGSEDPFDPGCTASPHSSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G7B4</accession>
    <name>A0AAD5G7B4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PB1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_024637</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010861">
        <property type="protein sequence ID" value="KAI7730003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G7B4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05992">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045012">
        <property type="entry name" value="NLP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053793">
        <property type="entry name" value="PB1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000270">
        <property type="entry name" value="PB1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003035">
        <property type="entry name" value="RWP-RK_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32002:SF49">
        <property type="entry name" value="BILE ACID:SODIUM SYMPORTER_ARSENICAL RESISTANCE PROTEIN ACR3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32002">
        <property type="entry name" value="PROTEIN NLP8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00564">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02042">
        <property type="entry name" value="RWP-RK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00666">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51745">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="PB1" evidence="5">
        <location>
            <begin position="286"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7730003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="394" mass="45017" checksum="4EA02251590EA496" modified="2024-05-29" version="1" fragment="single">DHIEEALKVVGESYDLALAQVWIPYEDKTRVFLPSSLEDTQTKQLLAIKLTGCYYAMKDSNLFEPYFRFGDVTPRAIAGDFPLVTLQDYKSRYILDVRSQLVMWCGVGGFPPTSALVICLRSNDTSDCNYVFEFMWKKDSNFIHLEALLLTLKRCLPRFKFASGVEIGNELNVHIEGDATGAELGDKIDIHTEDDGNKKFKIFQGKRSVEHYEKQFGKTMKNAAKNLNVSLSTFKRKLKALGIKEWPGPNFLKRNKNDSSIIQFNTNEEDNGAIEDTSAINLNKNVLTVKAEYEDDIIKFNLPISQATFVHVENEIGKKFKLSAKTYKLKYLDEDGDWITLTSDEELTDCIKSSRKSNRIVVRMRVLPSRQPICSRVQEEKKDIPCYNQPSMAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G7I6</accession>
    <name>A0AAD5G7I6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">S-adenosyl-L-methionine-dependent methyltransferases superfamily protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_007794</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010452">
        <property type="protein sequence ID" value="KAI7731424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G7I6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.110">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37211">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37211:SF1">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7731424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="342" mass="39528" checksum="50AD2E00E9FDD42D" modified="2024-05-29" version="1" fragment="single">AKLEKQNNKHNQGNTNTCDSIEPPTETKNSNQRHKWGRTARNKETNISPKGDISYLQKFFLTYVGGRAPLHLQEDFCGTALLSTEWLRSDSRRTSIGLDLDVEALDYCMENNVNKIGADLSSRIFLYHGNVLQPQEAKLVKSNLQNIMHNVTLEENENDSEPITNPEALPPRDIVCAFNYSCCCLHSRHELVSYFKNALQALSKKGGIFVMDLYGGTSSECELRMQRKFPNFTYTWEQAGFDIVQRKTRISLHYTLHKHQNKKLRHAFSYSWRLWSLPEIKDCLEEAGFRSVHFWIRQMPDSENIKSIQGFGDGKDIKYEEVTSFQQKDSWNAYIVELCVKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GBJ6</accession>
    <name>A0AAD5GBJ6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_006302</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009734">
        <property type="protein sequence ID" value="KAI7734411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GBJ6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36800">
        <property type="entry name" value="POLYAMINE-MODULATED FACTOR 1-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36800:SF1">
        <property type="entry name" value="POLYAMINE-MODULATED FACTOR 1-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="54"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7734411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="95" mass="10656" checksum="3147C9E8CD0100EB" modified="2024-05-29" version="1">MASSPIDNSHLLPLSDGHSPLSSVIYELSQQVQAGMENMLKMISEIDQSSGGILEDIEKCKDSALERKRTLEEDKDRFQKAAYSVLDMLNNRENN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GBK3</accession>
    <name>A0AAD5GBK3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mitochondrial pyruvate carrier</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_017817</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Mediates the uptake of pyruvate into mitochondria.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Mitochondrion inner membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the mitochondrial pyruvate carrier (MPC) (TC 2.A.105) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009476">
        <property type="protein sequence ID" value="KAI7735459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GBK3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006850">
        <property type="term" value="P:pyruvate import into mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005336">
        <property type="entry name" value="MPC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14154">
        <property type="entry name" value="UPF0041 BRAIN PROTEIN 44-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03650">
        <property type="entry name" value="MPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7735459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="113" mass="12745" checksum="2C9ED019B3AF2858" modified="2024-05-29" version="1" fragment="single">RKRTCLQVVTNHNNNIPWLVVSNAANQRLLVLLSHRRRSVLIPRYYYMASFRAFLNSPVGPKTTHFWGPVANWGFVVAGLVDMQKPPEMISGNMTAALTTLIPIDGDTDDDHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GEE9</accession>
    <name>A0AAD5GEE9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">pyruvate decarboxylase</fullName>
            <ecNumber evidence="6">4.1.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">M8C21_026622</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">AA19_3_7</strain>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 2-oxocarboxylate + H(+) = an aldehyde + CO2</text>
            <dbReference type="Rhea" id="RHEA:11628"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17478"/>
            <dbReference type="ChEBI" id="CHEBI:35179"/>
            <dbReference type="EC" id="4.1.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="12">Binds 1 Mg(2+) per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>a metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:25213"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the TPP enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.1" evidence="6"/>
    <dbReference type="EMBL" id="JAMZMK010008639">
        <property type="protein sequence ID" value="KAI7739287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GEE9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004737">
        <property type="term" value="F:pyruvate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000949">
        <property type="term" value="P:aromatic amino acid family catabolic process to alcohol via Ehrlich pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036293">
        <property type="term" value="P:response to decreased oxygen levels"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02005">
        <property type="entry name" value="TPP_PDC_IPDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07038">
        <property type="entry name" value="TPP_PYR_PDC_IPDC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1220:FF:000009">
        <property type="entry name" value="Pyruvate decarboxylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000017">
        <property type="entry name" value="pyruvate decarboxylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000021">
        <property type="entry name" value="Pyruvate decarboxylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012110">
        <property type="entry name" value="PDC/IPDC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012000">
        <property type="entry name" value="Thiamin_PyroP_enz_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047214">
        <property type="entry name" value="TPP_PDC_IPDC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047213">
        <property type="entry name" value="TPP_PYR_PDC_IPDC-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43452">
        <property type="entry name" value="PYRUVATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43452:SF6">
        <property type="entry name" value="PYRUVATE DECARBOXYLASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00205">
        <property type="entry name" value="TPP_enzyme_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036565">
        <property type="entry name" value="Pyruvt_ip_decrb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="11" id="KW-0456">Lyase</keyword>
    <keyword evidence="9 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 13" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme N-terminal TPP-binding" evidence="16">
        <location>
            <begin position="42"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme central" evidence="14">
        <location>
            <begin position="239"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme TPP-binding" evidence="15">
        <location>
            <begin position="449"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="480"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="507"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="509"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR036565-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU362132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAI7739287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="603" mass="64937" checksum="906F36B1764FC998" modified="2024-05-29" version="1">MDTKIGSVQAVAPNGDVCCPPLHGGFSPVQSSSLPFNSSEATLGRHLARRLVQIGVSDVFSVPGDFNLTLLDHLLAEPGLNLVGCCNELNAGYAADGYARSRGVGACVVTFTVGGLSVVNAIAGAYSENLPLICIVGGPNSNDYGTNRILHHTIGLPDFSQELRCFQTVTCYQAVVNNLEDAHELIDTAISTALKESKPVYISISCNLPAIPHPTFSREPVPFSLSPKLSNQMGLEAAIETAADFLNKAVKPVMVGGPKMRVANACEAFVEMADSCGYAVAVMPSGKGLVPEHHPHFIGTYWGAVSTTFCAEIVESADAYLFAGPIFNDYSSVGYSLLVKKDKAIIVQPDRVVVGNGPAFGCVLMKDFLVGLAKKLKKNTTAYENYHRIYVPEGHPVKSDPKEALRVNVLFEHIQNMLSGDTAVIAETGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQAATGKRVIACIGDGSFQVTAQDVSTMIRCGQKTIIFLINNGGYTIEVEIHDGPYNVIKNWNYTGFVDAIHNGEGKCWTSKVFCEEELVKAIETATEEKKDSLCFIEVIVHKDDTSKELLEWGSRVSAANSRPPNPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GEH7</accession>
    <name>A0AAD5GEH7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_000208</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008802">
        <property type="protein sequence ID" value="KAI7738504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GEH7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7738504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="127" mass="15034" checksum="0C3BB08946D744AB" modified="2024-05-29" version="1" fragment="single">SLMLCRLPLDLTHPVIPPPSLRLSHFSYHYFFPYFRSLRLEKERHVRTKYSAVAGWCTVISNRFLRTEIDGNNIPRPLAMSVDNTSPDRISLFISCRFQQLMNLFWISRIRHHRLVLADLAFCLIFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GF44</accession>
    <name>A0AAD5GF44_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_015641</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008524">
        <property type="protein sequence ID" value="KAI7740080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GF44"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7740080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="32" mass="3455" checksum="0F2B03FBC4148B0D" modified="2024-05-29" version="1">MAMASRLWASKAASYLKISTFNRAFASGSKVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GFX1</accession>
    <name>A0AAD5GFX1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_018260</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008549">
        <property type="protein sequence ID" value="KAI7739884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GFX1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042082048" description="Secreted protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7739884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="80" mass="9182" checksum="3B0DEDA239E74A7D" modified="2024-05-29" version="1">MSTFSLSAHVFLSTQLSLSLLLQTLFSSCHRLHLRLLPPSSAFICYGHLLKTKKDRFDDGDEPNYHLRYRHCFVDSSPPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GGF8</accession>
    <name>A0AAD5GGF8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ras-related protein RABC1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_031496</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Endomembrane system</location>
            <topology evidence="4">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008676">
        <property type="protein sequence ID" value="KAI7739093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GGF8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00037868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7739093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="119" mass="13446" checksum="8EBC23D39FB1CD00" modified="2024-05-29" version="1">MVYDVTRRETFTNLSDIWAKEIDMHSTNQDCIKMLVGNKVDKESERVVTKKEGIEFAREYGCLFIECSAKTRVNVEQCFEELVLKILDTPSLTAEGSTAVKRNIFRQKPAADASTSACC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GJC7</accession>
    <name>A0AAD5GJC7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Reticulon-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_013485</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Endoplasmic reticulum membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007396">
        <property type="protein sequence ID" value="KAI7744985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GJC7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009617">
        <property type="term" value="P:response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003388">
        <property type="entry name" value="Reticulon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045064">
        <property type="entry name" value="Reticulon-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10994">
        <property type="entry name" value="RETICULON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10994:SF177">
        <property type="entry name" value="RETICULON-LIKE PROTEIN B15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02453">
        <property type="entry name" value="Reticulon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50845">
        <property type="entry name" value="RETICULON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="Reticulon" evidence="7">
        <location>
            <begin position="60"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7744985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="238" mass="27183" checksum="9C04B0C61CC00849" modified="2024-05-29" version="1">MSDAAVEDKIMAATEKKIEELSAEPKLVQEKNEENKSVSRKKHLFGRQKPLHAALGSGKTADILLWRQKQLSGAIFVSATVIWLLFERIGYHLLPFLCHFLILTLAILFLWSNLSSFVNRSPPKFPDIRLSQEMCDCIALLAMDKVNRVCLYLREMTTGRDLKRFMSVIFTLWVVSVIGGWFKFLTLVYILFVLMLSAPLLYERNEGLVDAYGEKAGNEIMAALEKLPLPFFKSNKQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GL37</accession>
    <name>A0AAD5GL37_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_020664</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006873">
        <property type="protein sequence ID" value="KAI7746935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GL37"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7746935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="88" mass="9780" checksum="9FC0706C53D42BF0" modified="2024-05-29" version="1" fragment="single">MGTDKQSVGILDTINMETVRTMLTHTYPYPHEHSRHAIIAVYSMDISGGFIPSSKLSVDGLFLGLWYIGVVSRMAGRRPNILTVLQNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GLH7</accession>
    <name>A0AAD5GLH7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_023677</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007287">
        <property type="protein sequence ID" value="KAI7745329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GLH7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7745329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="130" mass="14480" checksum="76B09AB958F85B37" modified="2024-05-29" version="1" fragment="single">LEVSNGTNTPVRNDASLPEYNRNDRGRVLEVPQRLWSSPSPQKGYDSGGGVNKWRPRAKLRIDLSAIKKVIVSELDFSELEGLRTYTFAAIEGSDIESEAYEISIRGKRKVHVQTSKTVTNTTCGPEDTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GLY1</accession>
    <name>A0AAD5GLY1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_017313</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007538">
        <property type="protein sequence ID" value="KAI7744433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GLY1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37213">
        <property type="entry name" value="SUBTILISIN-LIKE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37213:SF1">
        <property type="entry name" value="SUBTILISIN-LIKE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7744433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="81" mass="8947" checksum="02144BC08292E5D8" modified="2024-05-29" version="1">MATGWKKTLGNIRSAVGNAMGGLRGGSNLASWVVAGTLAYFLWIKPSQELKREQEERAAITAANDRYRYVEKIKPIPVLQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GMF4</accession>
    <name>A0AAD5GMF4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mediator complex subunit 15 KIX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_030585</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006994">
        <property type="protein sequence ID" value="KAI7746334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GMF4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.246.20:FF:000003">
        <property type="entry name" value="Mediator of RNA polymerase II transcription subunit 15a"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.20">
        <property type="entry name" value="Coactivator CBP, KIX domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036529">
        <property type="entry name" value="KIX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036546">
        <property type="entry name" value="MED15_KIX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044661">
        <property type="entry name" value="MED15a/b/c-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33137">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 15A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33137:SF4">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 15A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16987">
        <property type="entry name" value="KIX_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47040">
        <property type="entry name" value="Kix domain of CBP (creb binding protein)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mediator complex subunit 15 KIX" evidence="4">
        <location>
            <begin position="51"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="Mediator complex subunit 15 KIX" evidence="4">
        <location>
            <begin position="171"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="Mediator complex subunit 15 KIX" evidence="4">
        <location>
            <begin position="283"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="Mediator complex subunit 15 KIX" evidence="4">
        <location>
            <begin position="410"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="domain" description="Mediator complex subunit 15 KIX" evidence="4">
        <location>
            <begin position="783"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="721"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="985"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1040"/>
            <end position="1064"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="17"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="727"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="987"/>
            <end position="1001"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1013"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1044"/>
            <end position="1064"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7746334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="1064" mass="120981" checksum="486B6A1F9E2D2F42" modified="2024-05-29" version="1" fragment="single">VNSLAADHNLTKRRNNDYQNPNSNNWASHHLMDMSNGSPSVTVAVGDWREPLQRERIVNKIMDTLKKNLPFSRHDRLQELKEIAERVEHEIYTAATTQSEYSRKICLNMLTMETRLQNHMSISMHSNSSAISVNPSVPVDSTANGGDWQQEVYQKGSTACRVTAKDREQDKHLTYSGQEGLQELKKIAVRFEERIYTASTSQSDYLRKISLKMLTMENRSQNNAANDTVNGPNCTFILFDIWGILLKLVIVPDLTFAYSSENFLITYLNFHIGDVTMEFGDWRTPLQRERIVNKIMDTLKTNLPFSRYDRLQELKKIAHRVEQEIYTAVTSQSEYTRKICFMMLTMETRLQNPMSDSDPTSAPLDSTANCGDWQEEVYQKIKTMKDLYLLDSRLSHGASGAVGDPTLESGDWKAQLQADSRQRIVNKILDTLKMCLPFSGQEELQDIAVWFEEKTYNAATTQSDYLRKISLKMLKMESRFQDSMSDPMQSNSAVNSVNPSVPLASIAQTSNPNGEDWQEEVYQKILAMKDLYLLDLNNIQQKLLSKLQQHESLPQQIKNEQHEKLKTFKNMLERFMLFLKIPKHEILASYKDRLGTYETQIVKVINLYRQRPAASQLQAQALPPSHMKYLQQSQQTHSQSAQVESHENQVNLQGSMTHMLHSNKMPIKQENQQSSANLLPTEVQLHPHLQQQVGMQQRLPTAGYFQQQHVIDMQKRLCPQKRAMPEASSTSLDSTAQNGDPNSGESQKEVCQKIKTMKDMYLLDLRPTQGPSGAVGDPTMESSDWRAQLQVDSRQRIVNKIYATLKMHLPFSGHEGLQELNKIAVRFEEKIYSIATSQDDYLRKISLKMLNTENRSQNPVLYPMQSNSTVNGVYPSSPGFQVMQQVINQRQQLPIPVPSNNTQQMTSQSMHSNIGSTRIQGSSSFSSAFPPGRGLQQPTMGNVVGQNSNLHNIQIMSSVQGNQLIGQQYNYSNMQQQRLISEQNKISSLQQPSQPRLSPTQGASGAGPNDSRFTGKDTKQDDDYLRKISLKMLTMENRFPVSDPMQSNSAAPQNLMDSIQSCLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GNR3</accession>
    <name>A0AAD5GNR3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Cytochrome c domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">M8C21_033658</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">AA19_3_7</strain>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>heme c</name>
            <dbReference type="ChEBI" id="CHEBI:61717"/>
        </cofactor>
        <text evidence="15">Binds 1 heme c group covalently per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Component of the ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII), a multisubunit enzyme composed of 3 respiratory subunits cytochrome b, cytochrome c1 and Rieske protein, 2 core protein subunits, and additional low-molecular weight protein subunits. The complex exists as an obligatory dimer and forms supercomplexes (SCs) in the inner mitochondrial membrane with cytochrome c oxidase (complex IV, CIV).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytochrome c family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006985">
        <property type="protein sequence ID" value="KAI7746428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GNR3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006122">
        <property type="term" value="P:mitochondrial electron transport, ubiquinol to cytochrome c"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.760.10:FF:000002">
        <property type="entry name" value="Cytochrome c1, heme protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.100:FF:000003">
        <property type="entry name" value="Cytochrome c1, heme protein, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.760.10">
        <property type="entry name" value="Cytochrome c-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.100">
        <property type="entry name" value="Cytochrome c1, transmembrane anchor, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009056">
        <property type="entry name" value="Cyt_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036909">
        <property type="entry name" value="Cyt_c-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002326">
        <property type="entry name" value="Cyt_c1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021157">
        <property type="entry name" value="Cyt_c1_TM_anchor_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10266">
        <property type="entry name" value="CYTOCHROME C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10266:SF3">
        <property type="entry name" value="CYTOCHROME C1, HEME PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02167">
        <property type="entry name" value="Cytochrom_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00603">
        <property type="entry name" value="CYTOCHROMEC1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46626">
        <property type="entry name" value="Cytochrome c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81496">
        <property type="entry name" value="Cytochrome c1 subunit of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase), transmembrane anchor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51007">
        <property type="entry name" value="CYTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0249">Electron transport</keyword>
    <keyword evidence="4 15 16" id="KW-0349">Heme</keyword>
    <keyword evidence="11 15 16" id="KW-0408">Iron</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 15 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Cytochrome c" evidence="17">
        <location>
            <begin position="125"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="binding site" description="covalent" evidence="15">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>heme c</name>
            <dbReference type="ChEBI" id="CHEBI:61717"/>
        </ligand>
    </feature>
    <feature type="binding site" description="covalent" evidence="15">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>heme c</name>
            <dbReference type="ChEBI" id="CHEBI:61717"/>
        </ligand>
    </feature>
    <feature type="binding site" description="covalent" evidence="15">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>heme c</name>
            <dbReference type="ChEBI" id="CHEBI:61717"/>
        </ligand>
    </feature>
    <feature type="binding site" description="covalent" evidence="15">
        <location>
            <position position="261"/>
        </location>
        <ligand>
            <name>heme c</name>
            <dbReference type="ChEBI" id="CHEBI:61717"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00038521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR602326-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAI7746428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="342" mass="37777" checksum="1F2F642359EF79F4" modified="2024-05-29" version="1">MAGGRGLYHLLRGRLHSRIDFLIWMLCMAKYFDLSAYHFMSFCRSIDYIHNQAPPALSSLISKGDQDGAGSAGVKSLRAIALLGAGLSGILSFTTIAAADEAEHGLESPNYPWPHQGILSSYDHASIRRGHQVYTQVCASCHSMGLISYRDLVGVAYTEEETKAMAAEIEVVDGPNDEGEMFTRPGKLSDRFPQPYANEQAARFANGGAYPPDLSLITKARHNGQNYVFALLTGYRDPPAGVTIREGLYYNPYFPGGAIAMPQMLIDGAVEYEDGTPATEAQMGKDVVSFLSWAAEPEMEERKLMGFKWIFVLSLALLQAAYYRRLRWSVLKSRKLVLDVVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GPJ0</accession>
    <name>A0AAD5GPJ0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sulfotransferase</fullName>
            <ecNumber evidence="3">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_022491</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the sulfotransferase 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010006121">
        <property type="protein sequence ID" value="KAI7750582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GPJ0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11783">
        <property type="entry name" value="SULFOTRANSFERASE SULT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sulfotransferase" evidence="4">
        <location>
            <begin position="13"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7750582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="168" mass="19701" checksum="9976AD28D3235596" modified="2024-05-29" version="1">MSKLRSKDLPPLTLEEAFELFCLGVSEFGPFWEHVLSYWRASLESPDKILFLKYEEIKKQPEMVVRMLAAFLGKPMTLEEEEKGVVREIVKLCSFENLSNLEVNKNGVQKFVETLEVANRDYFRKGEIGDWKNYLSEEMKERIDGITYEKFHGSGRSTLERVMRSDYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GQ89</accession>
    <name>A0AAD5GQ89_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Inositol polyphosphate-related phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_000095</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the inositol polyphosphate 5-phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006501">
        <property type="protein sequence ID" value="KAI7748556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GQ89"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004445">
        <property type="term" value="F:inositol-polyphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034485">
        <property type="term" value="F:phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004439">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045849">
        <property type="entry name" value="IP5P_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000300">
        <property type="entry name" value="IPPc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45666:SF48">
        <property type="entry name" value="ENDONUCLEASE_EXONUCLEASE_PHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45666">
        <property type="entry name" value="TYPE IV INOSITOL POLYPHOSPHATE 5-PHOSPHATASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22669">
        <property type="entry name" value="Exo_endo_phos2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Inositol polyphosphate-related phosphatase" evidence="3">
        <location>
            <begin position="30"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7748556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="206" mass="22854" checksum="AA4408083D68E4EE" modified="2024-05-29" version="1" fragment="single">VMWPGLVSSTKILNKMLSSNVVTDFPTSPEQVNITQNYKFQEVVPLTTPNILGLKKKKVSKKWNSLIRKTLNRSSKITKPNSDNSDPDNKFRCLISKRMVGLLISVWLRSDLYQHVKNANVSCVGCGIMGRLGNKGSVSVRFQLGGTSFCFVCTHLASGGGEGDKQIRNSNAIEIFSRTSFPTTNGRSSVVLPKRILDHEYVYVLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GQM5</accession>
    <name>A0AAD5GQM5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_007565</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006526">
        <property type="protein sequence ID" value="KAI7748408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GQM5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040411">
        <property type="entry name" value="At5g23160-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34379">
        <property type="entry name" value="OS07G0553800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34379:SF6">
        <property type="entry name" value="PROTEIN 3F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="245"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="136"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="136"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="162"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7748408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="316" mass="35232" checksum="A5B4E8FC54D65AA4" modified="2024-05-29" version="1">MADIHKRKKKRGRSKLFKSCFRSFANDERPFLNNNNNSDSQSRLGEEDDVGVQDVNYERSSSNRSVPRLLKAVLFEPVMVKKMFSSSKSSTTSYTNNTIRKQLNDSNASCIESTDIEDSSSSRCSTLLSSSSRATSSLYSSSSSSSSLSSTNSTSRWLSEPKTLTSTDTANFNHQMSSNSSLPKPNLINSSSQTDSITLPRSSSTLSHNKALSQLDSIDSKTSVLSNPDKLKNNGNNWSTTRLCLFLLLSFIVLVVYGRIYAILCTSIWFYMVPGRRVKRVNSVINSSKVIDTESEQYKKRVIMAGLLDRTRNPLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GQU1</accession>
    <name>A0AAD5GQU1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_004500</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006394">
        <property type="protein sequence ID" value="KAI7749216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GQU1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7749216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="93" mass="10184" checksum="16741B4A0932F526" modified="2024-05-29" version="1" fragment="single">GNDDEYLSSCLSESVVEIYLILAGGNKDEIVLGLAMALLGCVIARTILRLLVNWRLPVFDPSALVTSLTTDGLGCNNENMFCWMLEARGLFDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GR67</accession>
    <name>A0AAD5GR67_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_007084</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005755">
        <property type="protein sequence ID" value="KAI7752110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GR67"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7752110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="62" mass="7319" checksum="AB66121C8DC3D8B7" modified="2024-05-29" version="1" fragment="single">VVFWKLITPKMLGMVMDDWPCKSDLKQEMRVMAVACASRKGSQEMSVRLRLIVERYRRQNDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GSC8</accession>
    <name>A0AAD5GSC8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">MI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_022333</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CWC22 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005505">
        <property type="protein sequence ID" value="KAI7753220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GSC8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.180:FF:000004">
        <property type="entry name" value="pre-mRNA-splicing factor CWC22 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050781">
        <property type="entry name" value="CWC22_splicing_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003891">
        <property type="entry name" value="Initiation_fac_eIF4g_MI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003890">
        <property type="entry name" value="MIF4G-like_typ-3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18034">
        <property type="entry name" value="CELL CYCLE CONTROL PROTEIN CWF22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18034:SF3">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR CWC22 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02847">
        <property type="entry name" value="MA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02854">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00544">
        <property type="entry name" value="MA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00543">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51366">
        <property type="entry name" value="MI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="MI" evidence="8">
        <location>
            <begin position="579"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="530"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="782"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="71"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="106"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="126"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="136"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="154"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="548"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="787"/>
            <end position="812"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7753220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="828" mass="96093" checksum="13BDFA64C9E85FA3" modified="2024-05-29" version="1">MERHRGRSEDRSDDEDKYRRRRNDEDGEVRKRGEKGKGRSDDEDDYKDRKRRDMNRNMSDEEDDKYKKRRGNDSRRRDEDDGRRNRKKSEGSKRRARYDESDDESGEIRKSGRKHEEESDDEDSKIRKRGRKGRDRRSDDEGDRKDRRRRDRYKHGSDDEDDKYKRRDDDGRRRDTKRDKRSNGDHKDRRRGDDVANKKSDKNGDTENLDEGPRFQKLAEMQEGMTNLGKTGGVYIPPFKLARMMSGVQDKSSMEYQRMTWDALRKSINGLVNKVNATNIKNIIPELFAENLIRGRGLFCRSCMKSQMASPGFTDVFAALVAVVNTKFPEVGDLLLRRIILQLQRAYKRNDKHQLLAAVKFIAHLVNQQVVHELIALELLTLLLENPTDDSVEVAVGFVTECGSILQDLSPKGLHGIFERFRGILHEGEIDKRVQFLIEGLFALRKAKFQGHSAVRPELDLVELEDQLTHEVSLLDKIDPEISLVTPPPSSHLHEPTAATTTTRTVDEYDIFKMDPDFLENEKRYEDLKKTILGDEEEPSDDDKGGEDSDDDEEEDESSEEEDEEQMRIRDETETNLVNLRRTIYLTIMSSVDFEEAGHKLLKIKLEPGQEMELCIMLLECCSQERTYLRYYGLLGQRFCMINKVHQENFEKCFVQQYSMIHRLETNKLRNVAKFFAHLLGTDALPWHVLAYIRLTEEDTTSSSRIFIKILFQELSEHLGIRLLNERLSDPSMQADFESIFPRDNPKNTRFSINFFTSIGLGGITENLREYLKNMPRLIMQQKPASESDGSDSSSSGSDSSSSGESESSSESSDSESDHKSSKRRRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GSR7</accession>
    <name>A0AAD5GSR7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Small EDRK-rich factor-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_025504</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005318">
        <property type="protein sequence ID" value="KAI7753700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GSR7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1050.10">
        <property type="entry name" value="At2g23090-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039713">
        <property type="entry name" value="At2g23090-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007513">
        <property type="entry name" value="SERF-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026939">
        <property type="entry name" value="ZNF706/At2g23090_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33788:SF20">
        <property type="entry name" value="GENOME ASSEMBLY, CHROMOSOME: A03"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33788">
        <property type="entry name" value="OS07G0114300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04419">
        <property type="entry name" value="SERF-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118359">
        <property type="entry name" value="Expressed protein At2g23090/F21P24.15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Small EDRK-rich factor-like N-terminal" evidence="2">
        <location>
            <begin position="3"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7753700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="40" mass="4233" checksum="B52332256ABB5A0D" modified="2024-05-29" version="1">MGGGNAQKSKTARERNLEKAKAASKGSQLEANKKAMNIQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GUP0</accession>
    <name>A0AAD5GUP0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_032827</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000479">
        <property type="protein sequence ID" value="KAI7756282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GUP0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040278">
        <property type="entry name" value="UPF0426"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35996:SF1">
        <property type="entry name" value="OS04G0528100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35996">
        <property type="entry name" value="OSJNBA0038O10.25 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26369">
        <property type="entry name" value="UPF0426"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="104"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7756282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="114" mass="12907" checksum="FA54E6578ACA5FB3" modified="2024-05-29" version="1">MASLCLISIPSWKSVGRKQTRNTSVRCANNNNNNNKCEANSFFLSPSDERILKQAFKEPVAFMGGMFAGVLRLDLNEDPLKEWVKRTVETSGITTNDEEDEQQLDTQPQEIEIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GVM4</accession>
    <name>A0AAD5GVM4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_029088</name>
        <name evidence="10" type="ORF">M8C21_029096</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005009">
        <property type="protein sequence ID" value="KAI7753956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAMZMK010005009">
        <property type="protein sequence ID" value="KAI7753964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GVM4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007623">
        <property type="term" value="P:circadian rhythm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009637">
        <property type="term" value="P:response to blue light"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22154">
        <property type="entry name" value="F-box_AtADO-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.50:FF:000021">
        <property type="entry name" value="Adagio protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000005">
        <property type="entry name" value="Putative LOV domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000050">
        <property type="entry name" value="Putative LOV domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011498">
        <property type="entry name" value="Kelch_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46175:SF5">
        <property type="entry name" value="ADAGIO PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46175">
        <property type="entry name" value="BACTERIOOPSIN TRANSCRIPTIONAL ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07646">
        <property type="entry name" value="Kelch_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0288">FMN</keyword>
    <keyword evidence="2" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="F-box" evidence="8">
        <location>
            <begin position="125"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="561"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7753956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7753964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="561" mass="61053" checksum="652999A995A23D63" modified="2024-05-29" version="1" fragment="single">RFLQCRGPYAKRRHPLVDSTVVSEIRRCLEDGVEFKGELLNFTKDGTPLMNRLRMTPIYGDDEFFTEVDLDLGPLTGTTAKGTVKSSDPFRSSLLSCRLTPDGTRTSSRGLSGILQLSDEVISLKILSRLTPRDIASVGSVCRRFYEVAKNEDLWRMVCQNAWGSETTRVLETVPGAKRLGWGRLARELTTLEAAAWRKLSVGGAVEPSRCNFSACAVGNRVVLFGGEGVNMQPMNDTFVLDLNSSKPEWQHVKVASPPPGRWGHTLSCVNGSNLVVFGGCGTQGLLNDVFLLDLDAKHPTWREISGLAPPLPRSWHSSCTLDGTKLIVSGGCADSGVLLSDTFLLDLSMEKPIWREIPVTWTPPSRLGHTLSVYGGRKILMFGGLAKSGPLRFRSSDVFTMDLSEDEPCWRSVTGSGMPGAGNPGGIAPPPRLDHVAVSLPGGRILVFGGSVAGLHSASQLYILDPTEEKPTWRMLNVPGLPPRFAWGHSTCVVGGTRAIVLGGQTGEEWMLRNDKFVLNSVSSSSTMFLGSHRRHLFDRGPASCLAVDDDFLFGAARWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GVP4</accession>
    <name>A0AAD5GVP4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_021056</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000179">
        <property type="protein sequence ID" value="KAI7757120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GVP4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="115"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="132"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="143"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="154"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7757120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="206" mass="23546" checksum="EF54F712EF137F19" modified="2024-05-29" version="1" fragment="single">MKKKKVKPTYNNAKDVAYIPDNDNDDNSEEDQEVNISVEVSKKALKENQSRSTVTMGELLSKTQRGVLSKKAGRPNSIKDRSKEQLNLVDEDDDWIFQDGLENDENMLMNSTKLDGLENDENEGDVMRSQADQDEEDEDIEEIIAANNNMNMDDSSKDSENEDDVHRLEIEQIQRPELGKAIEIPKSKKRGPTMLHVVHTRTVKER</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GVZ5</accession>
    <name>A0AAD5GVZ5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_025509</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010003932">
        <property type="protein sequence ID" value="KAI7754271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GVZ5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043312">
        <property type="entry name" value="AtBBR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47530">
        <property type="entry name" value="E3 UBIQUITIN LIGASE BIG BROTHER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47530:SF4">
        <property type="entry name" value="E3 UBIQUITIN LIGASE BIG BROTHER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17123">
        <property type="entry name" value="zf-RING_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="3">
        <location>
            <begin position="244"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="73"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="24"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="104"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7754271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="337" mass="37150" checksum="818A0A452F4BBEAE" modified="2024-05-29" version="1">MDNSKDQPTNTDENPTNITTTDSPPVIAADDNNNNNPNRPVTRTPFTNLSQVDADLALARTLQDQERAYMMLRMNNGGGGSDNESWETGSYVDDELGEHNSEYDGSEGDEEEEDAFDVHAHLDDVSGEEDRGGRGVELDPANFPNDEAYARALQDAEDQEMAARLLALTGINGMVTEEEDSMEDDIGNNSQDSYDDVDPDELSYEELIALGEVVGTESRGLSADSIASLRSIVYKTQSGSSETCVICRLDYEDGDNLILLSCKHSYHSECITNWCALFVAPRCRHLAALVDIDDGLFYVAARTNRQLLYCAKRPLVTFVWNVVLGRFLCSGLSWSTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GXM7</accession>
    <name>A0AAD5GXM7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Geranylgeranyl diphosphate reductase, chloroplastic</fullName>
            <ecNumber evidence="3">1.3.1.83</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Geranylgeranyl reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_023307</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the reduction of geranylgeranyl diphosphate to phytyl diphosphate, providing phytol for both tocopherol and chlorophyll synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>phytyl diphosphate + 3 NADP(+) = geranylgeranyl diphosphate + 3 NADPH + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:26229"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57533"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:75434"/>
            <dbReference type="EC" id="1.3.1.83"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">Cofactor biosynthesis; tocopherol biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Porphyrin-containing compound metabolism; chlorophyll biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the geranylgeranyl reductase family. ChlP subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.83" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010000694">
        <property type="protein sequence ID" value="KAI7755936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GXM7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102067">
        <property type="term" value="F:geranylgeranyl diphosphate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045550">
        <property type="term" value="F:geranylgeranyl reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015995">
        <property type="term" value="P:chlorophyll biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015979">
        <property type="term" value="P:photosynthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000083">
        <property type="entry name" value="Geranylgeranyl diphosphate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010253">
        <property type="entry name" value="BchP_ChlP_pln/prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011777">
        <property type="entry name" value="Geranylgeranyl_Rdtase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011774">
        <property type="entry name" value="Geranylgeranyl_Rdtase_pln/cyn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050407">
        <property type="entry name" value="Geranylgeranyl_reductase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02023">
        <property type="entry name" value="BchP-ChlP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02028">
        <property type="entry name" value="ChlP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02032">
        <property type="entry name" value="GG-red-SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42685">
        <property type="entry name" value="GERANYLGERANYL DIPHOSPHATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42685:SF13">
        <property type="entry name" value="GERANYLGERANYL DIPHOSPHATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0149">Chlorophyll biosynthesis</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0602">Photosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding" evidence="13">
        <location>
            <begin position="44"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00033069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00058147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00067953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7755936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="419" mass="45831" checksum="441C91D24687F42A" modified="2024-05-29" version="1">MAVITHPLHSLNIPFKAPNPRFTPQPKLTISATSNKHISGRKLTAAVIGGGPAGSSAAEALASGGVETYLFERSPSGAKPCGGAIPLCMLEEFSIPPDLIDRKVTHMKIISPSNLTVDFGKTLKSHEYISMLRREVLDSYLRSRAESKGVTLVKALVTNLDVPNNPNQPYIVNYTVNNRMKYLPVDVVVGADGANSRVAKAIGAGEYACAIAFQERIKLPDDKMEYYENLAEMYVGNDVSPDFYAWVFPKCDHVAVGTGTVCSKQYIKQYQQGVRSRVHPKISGGKVIKVEAHPIPEHPRPVRVRGRVALVGDAAGYVTKCSGEGIYFAAKSGRMCGEAIVKASEGGHRMIGESDLKREYLKQWDSKMTFESYLYKKLAKGNTWEDFKMVSNTVGSLIRCKLVGKGMETFDPNVLAKLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GXQ7</accession>
    <name>A0AAD5GXQ7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SPX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_025049</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000244">
        <property type="protein sequence ID" value="KAI7756864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GXQ7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016036">
        <property type="term" value="P:cellular response to phosphate starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052486">
        <property type="entry name" value="PHO1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004331">
        <property type="entry name" value="SPX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48477">
        <property type="entry name" value="PHOSPHATE TRANSPORTER PHO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48477:SF1">
        <property type="entry name" value="PHOSPHATE TRANSPORTER PHO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03105">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51382">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SPX" evidence="2">
        <location>
            <begin position="2"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7756864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="225" mass="26144" checksum="9B0A7F5562C91FFF" modified="2024-05-29" version="1">MVKFSKELEAQLIPEWKDAFVNYWVLKKHVKKVKLSRISKPNINDHDYGVSIFDPVRSFIRRLSSCNATNNDPPDHMLQVRNTSSEVQSEEGEDEEDDETELVDHLYSEEDEVKEFFEKLDEELDKVNQFYMNKETEFLERGDMLKKQLQILLDLQQLVDCRRRSNSFNSTASSGFLRSYSSGRTSDSGIYIITFTLCFLLVDVDDATKVCKMVLYPVSGVQIIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BJY2</accession>
    <name>A0AAD5BJY2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ribosome biogenesis protein BMS1/TSR1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_023976</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012205">
        <property type="protein sequence ID" value="KAI7724522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BJY2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034511">
        <property type="term" value="F:U3 snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000479">
        <property type="term" value="P:endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039761">
        <property type="entry name" value="Bms1/Tsr1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007034">
        <property type="entry name" value="BMS1_TSR1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12858:SF1">
        <property type="entry name" value="PRE-RRNA-PROCESSING PROTEIN TSR1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12858">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04950">
        <property type="entry name" value="RIBIOP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01362">
        <property type="entry name" value="DUF663"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribosome biogenesis protein BMS1/TSR1 C-terminal" evidence="2">
        <location>
            <begin position="340"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="250"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="250"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7724522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="648" mass="73317" checksum="35B82EFE4B306E1B" modified="2024-05-29" version="1">MAGGSRSQVNKSHKTRFASKSSRNIHKTSVKDKSKISKPDRNVMKGAKAARLQRNKMMREQKRATLLKEKRASSGTASAPRVIVSILHDFCLTKFQSVSVNQDLPSDLKRKNELKKTCISSLSSEFPEDCKFYPADSKDDLHKVHVSGAGDFQLGKIELLKDPCSLSVKKGDFMDADEVNDSQVIRTLLPDLMKQEPLLVENIPDPLEGEQTWPTEAEMAEADRLHKEKKQKKRTLPRGTSEYQAAWIVDESDVSGSDSDDGSDNGMVVDEGEYDYPGQGDGGKSDFEDDHASLKLRDSDDETEADDMMMDNENLTKEQIEEDIRKIKEAHAEDEEFPDEVDAPVDVPARKRFAKYRGLKSFRTSSWDPKESLPPEYARIFAFDNFTRTHKHVLSKALDMEQGSLDECVPANSYVTLHIREVPIDIASKLCILAKTMPVIACGLLQHESKISVLHFRLKKHETYSEPIKAKEELIFHVGFRQFITRPTFASDNFNSDKHKMERFLHAGRFSIASVYAPISFPPLPLIVMKSRGDSFAPALAAVGSLKCIDPDKIILKKIILTGYPQRVSKSKAAVRYMFHNPEDVRWFKPVEVWTKLGRRGRIKEPVGTHGSMKCILNGVLQQNDTVCMSLFKRAYPKWPQHWFPLST</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BK57</accession>
    <name>A0AAD5BK57_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_004564</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012171">
        <property type="protein sequence ID" value="KAI7724687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BK57"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000290">
        <property type="term" value="P:deadenylation-dependent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033962">
        <property type="term" value="P:P-body assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039900">
        <property type="entry name" value="Pat1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21551:SF24">
        <property type="entry name" value="PROTEIN PAT1 HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21551">
        <property type="entry name" value="TOPOISOMERASE II-ASSOCIATED PROTEIN PAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="309"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="397"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="108"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="317"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="414"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7724687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="771" mass="84680" checksum="8CC74C6B223D93E5" modified="2024-05-29" version="1">MERSVTNSNTILGPSLSSDKLFDASQYAFFGQDSLDKADFGSLEVEEEDKPLNGAGDDEYRLFDRDEESGVGSLSEMDDLSTIFSKLNRTVSGPRHPGVIGDRGSGSGSISRESSSASEWLQERELTDWMDQHISNTDNYQGNRRWSSQPHLHSDSKPLYRATSYPEEQHQFFSEPFLVPDPSFPSFPSISQPDISSPHQHSHLLNLSPTITNSQLPFLENNGSMHLSGGLHRSRGYGGSGSHLIPPGLSRYSQPANNWANQMLHVDHAGLLSNTFQQKLLHNGSLPPHFMSPHHHSVGPFSPLQLYPLPSRPLHLSNHKTKTAQKGSGRHGSGRLSRQGSDGSNKKSDKFRVQFSSKYMNSEEIESILKVQHAATHSNDPYIDDYYHQARVAKGSDSKSKTRFCPAHLKDSPSKSSRNSTEPKNSFNIDAHGRISLPFIRRPQPLLEVDPPAGTEGKSSVRSLEQEPMFAARITIEDCLCLLLDVDDIDRLLQFNPPQDNGAQIRQKRKILLEGLATSLQLVDPLGKKSISVKLTPKDDIVFLRIASLPKGQKLISKYLQLLSPKSELARIVCMTIFRHLRYLFGGLPSDHEASLAVSGLAKTVSTCIGSMDLNSLSACLAAVVCSSEQPPLRPLGSPSGDGASVILKAVLDRATQLLSNPQGLADPGLQNPKLWQASFDAFFGLLTKYCLSKYDSLVQAMCIQTPPSAEMSVSEAAGAISREMPVELLRASLPHTDNNQRKMLVNFSQRSMQVAGHGEAGGQVKPESVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BL10</accession>
    <name>A0AAD5BL10_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_029630</name>
        <name evidence="3" type="ORF">M8C21_031627</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011984">
        <property type="protein sequence ID" value="KAI7725287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAMZMK010010204">
        <property type="protein sequence ID" value="KAI7732591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BL10"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45688">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45688:SF13">
        <property type="entry name" value="ALANINE--GLYOXYLATE AMINOTRANSFERASE 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7725287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7732591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="159" mass="17321" checksum="7CDFF3FCEAFE2473" modified="2024-05-29" version="1">MAKGIGNGIPLGAVVTTPEVAKVLTRRSYFNTFGGNPVCTAAGHAVLKVIERENLQENAHVVGSYLKERLTALKDKHEIIGDVRGRGLILGVELVTDRKLKTPAKTEILEIMETMKDLGVLVGKGGFYGNVFRITPPLCFTKEDADFLVDAMDYSMSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BQJ2</accession>
    <name>A0AAD5BQJ2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_016228</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011317">
        <property type="protein sequence ID" value="KAI7727788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BQJ2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042282696" evidence="1">
        <location>
            <begin position="26"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7727788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="167" mass="18314" checksum="DCA92B6D283FF4AE" modified="2024-05-29" version="1" fragment="single">PHPFIYVPSVLSSLFFLLSSPSLNNRPPLPGKKKTCPRRKVVGSLLLTSGHDLSSVVTCRLHRLHGVDGDGPPYVVCGGGLTVPVRRWYRALIATTKMQKTRFAALINGMILAHSSPAISISAQENRLLAIFSGSGHLRGEELMDCMEFKSFVRSRCDLVKNDDGKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BSE9</accession>
    <name>A0AAD5BSE9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_033787</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011157">
        <property type="protein sequence ID" value="KAI7728732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BSE9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47542">
        <property type="entry name" value="ACYL-COA N-ACYLTRANSFERASES (NAT) SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47542:SF2">
        <property type="entry name" value="ACYL-COA N-ACYLTRANSFERASES (NAT) SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7728732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="154" mass="17831" checksum="0C99DD1235590607" modified="2024-05-29" version="1">MELTELNRKSTNWAKVVDEIVKLEKKIFPKHESLARSFDDELKKKNSGLLYLQINEEIAGYVMYSSPSSLCSVITKLAVKEKYRRQGHGEALMKAAIEKCRSKRINRISLHVDPLRSSAVNLYKKLGFQIDTLVTAYYSSDRDAYRMFIDFDNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BV55</accession>
    <name>A0AAD5BV55_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_004507</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010809">
        <property type="protein sequence ID" value="KAI7730293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BV55"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47950:SF49">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47950">
        <property type="entry name" value="CYTOCHROME P450, FAMILY 76, SUBFAMILY C, POLYPEPTIDE 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0349">Heme</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7730293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="391" mass="44724" checksum="232414BF60A42A7A" modified="2024-05-29" version="1" fragment="single">FLVDNNLSFPLFVLSTISFIFLIVKQFKSFSLKSIKHLPPSPPRLPIIGNLHQVGSKPHISFANIAKEYGPLIYVRLGNQPVVIASTPETAMEILKTQDRFLSSRDVPNAFKQPSLLPHSLIWSECNQTWKKLRSLCRTKMFSTKALEAQSILREEKIGHMLDFLHSKQGEVTNIEDVVFTTLFNTLSSIIFAKDLIDLTDDYGSSDELKATLHKIIEFGGRVIDFGSFFPNLERFDLHGIRKGTMKHFLKAFSYWEYIIEERRSQVNSSSWSSNQAQTFVDRLLENGFSNNQIHQLVMELFVAGTNTTTSTVVWVMSELVRHQEVMFKIEDEMKKEIQSEKINPTHISKLSYLQACIKETMRLHPPVPLLLPHMAAETCEVMGYTIPKNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BW58</accession>
    <name>A0AAD5BW58_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_006243</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010754">
        <property type="protein sequence ID" value="KAI7730530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BW58"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968">
        <property type="entry name" value="CENTROMERE PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968:SF35">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIN-7D, MITOCHONDRIAL ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="3 5" id="KW-0505">Motor protein</keyword>
    <keyword evidence="1 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="8">
        <location>
            <begin position="1"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="214"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="126"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="314"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="383"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="488"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="215"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="243"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7730530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="653" mass="73092" checksum="B64FC17D9CC85A9E" modified="2024-05-29" version="1" fragment="single">MIESSAHGDEYDGVIFSQLNLIDLAGSESSKTETTGLRRKEGSYINKSLLTLGTVIGKLSEGKASHVPYRDSKLTRLLQSSLSGHGHVSLICTITPASSNMEETHNTLKFASRAKHVEIYASRNKIIDEKSLIKKYQREISVLKQELDQLRSGMLVGVNPEEIMSLKQKLEEGQVKMQSRLEEEEEAKAALMSRIQRLTKLILVSSKNAIPGLTDNGSMLVQSEDPSGTPSEISYELKHRRSSSNLSAAGSTITESTQAGDLNNGSGDGAKLPTGVISIDQMDLLVEQVKMLAGEIAFSSSTMKRLVEQSANDPESSKTQVENLEREIEEKRRQMRVLEKQIIESNEASISNTSLADMQQTMMRLMTQCDEKDFELEIKTADNRILQEQLQNKCSENKELQERNTLLEQQLAAAQSEKAMQSSRHHGSEENIDELRKKIKIQEVKSEKLKREHVKILEENSGLSVQNQKLSEEASYAKELASAAAVELKNLAAEVTKLSLQNAKLEKELVAARGGNRPKPGRNGRLSGWVNDEFDLDLEDLRRELHTMKQREVNLEAALAEKERSENEYMKKAEESKKKEAILENDLANMWVLVAQLKKEVNEGHVERNEAVNDVRLDNNGDVDNSVKETKVLDVQQVVHDVPKEENLVARLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BX09</accession>
    <name>A0AAD5BX09_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Mediator of RNA polymerase II transcription subunit 6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Mediator complex subunit 6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">MED6</name>
        <name evidence="8" type="ORF">M8C21_020575</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the Mediator complex subunit 6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010871">
        <property type="protein sequence ID" value="KAI7729981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BX09"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.580:FF:000002">
        <property type="entry name" value="Mediator of RNA polymerase II transcription subunit 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.580">
        <property type="entry name" value="Mediator complex, subunit Med6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007018">
        <property type="entry name" value="Mediator_Med6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038566">
        <property type="entry name" value="Mediator_Med6_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13104">
        <property type="entry name" value="MED-6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04934">
        <property type="entry name" value="Med6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 6" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="201"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="217"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU364143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7729981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="254" mass="28075" checksum="0032E20B51CAE35E" modified="2024-05-29" version="1">MAGTPMLPANLAAGGGNMDGNAAAAPPTPGTDMTGICFRDQLWLNTYPLDRNLVFDYFALSPFYDYTCNNEQLRMRSIHPLDISHLSKMTGIEFMVSEVMEPHLFVMRKQKRDAPEKVTPMLTYYVLDGSIYQAPQLCNVFAARVGRALYYISKAFTTAASKLEKVGYVDSEDDSTSHEPKATKETIDFKEVKRVDHILASLQRKLPAAPPPPPFPEGYTPPTAEGEQGPEAEQADPSVDPILDQGPAKRIKYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BXN3</accession>
    <name>A0AAD5BXN3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">GTD-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_022085</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010415">
        <property type="protein sequence ID" value="KAI7731643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080115">
        <property type="term" value="F:myosin XI tail binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007656">
        <property type="entry name" value="GTD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039306">
        <property type="entry name" value="MYOB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31448">
        <property type="entry name" value="MYOSIN-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31448:SF39">
        <property type="entry name" value="MYOSIN-BINDING PROTEIN 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04576">
        <property type="entry name" value="Zein-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51775">
        <property type="entry name" value="GTD_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="GTD-binding" evidence="7">
        <location>
            <begin position="1074"/>
            <end position="1172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="156"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="217"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="407"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="662"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="751"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="834"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="942"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1033"/>
            <end position="1053"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="1080"/>
            <end position="1174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="177"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="223"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="243"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="407"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="662"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="780"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="834"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="858"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="949"/>
            <end position="980"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="981"/>
            <end position="990"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="995"/>
            <end position="1009"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7731643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="1308" mass="147657" checksum="D7E00C17626AA065" modified="2024-05-29" version="1">MVFDVLDVRRRRVGLFAYQIDISHLLYCNLHDALVDVREIHWKEKSTGGQQPVWSNAVGSSGSKVTTKPPLPRVARHGRSKRLKNKVKHRHRDEFLFSDNDDCGLKVVGISERSLKSDADGVVNQYKVLGRHSHSTPDLKSAFLLEAHLDECSRTRSRTRSASLTPSKIGKDINPLSAMNSKTDGSLDSQNRYNIFSRNSFSASDLGSAVRLEMQDNNRLRQRSASLTPNTSSGYGLGEHQRPKNVHRKVKKHKKISSTNMYGCLDDSVVSSNTRKHHNRSKIPRRHSYSVLQLGGDILLNMPTDESLNGSVFYGLEDDKKAKVHSPEPERINVTQFPQRTDEDSSEYDLPIAFIDASKWYGSVDDSAVMRNTSIKYQNSNPRRHSHSIFDLGCEILLNMPVNEQPNNLKFQTRPNRSANSAKFCRRKTKRGKKSFSCSKKKSGYESLDDLVVTFNSDINDRNKDPRRYSYSAFELGDALLSRAQPIDRSSTNHVASATQNGAYNHGFGEPKPSGFQTVHNKNKGYESMDDLVVHEKYNDHRRHSYSAFEFGDVPMLNAQPIDKSSAYQSATMTQNGAYNHGFGEPKQSGFEMMNSYSHPIVFLNKNHGYGFMDDSVVTRNTGIDNHDHYHNQNRIPRRHSYSILQLGGDIILNMPNESISRSFGDSQKSTLQKVEKDKNSLPPKGSVKRFSPSYDDGSDLSIDKRNWHGSFDDSLIYRSVGSESQSNNPRRHSHSAFDLGRALLLEINESTGHGSTGHGSAGHRSTTHGSTGHGSGEHNRHHYPHHRKAKKNNSHLPPPCPMNALGNHNQYHNSNPRRHSYSAFELGSALLQNMQQSDTSSTRRSASVTPKIQIVNESKRPKLKKDEPHGYQSCDESGVSNSRDGRKRSTNLTRSYFSVSDLTSPLLLQMNLDKGLTRRSASLTPDCSLGQGFGDLNSLKFEAGSSSSHRKQKKNDRSKARRSKRSKAKKSKKHLRKVNNVKNTSASANAASDESYESFDDSDAYESLDDSDSQKRFKIFSRNSQMLSMIKKVQEKDPADSSSTNRPDLYPSGLEKIDLRSLDGSIASEIDYNGVEKVKQQAEDDVRSMTLLQSELEAERNAATVAANHAMNMITRLQQEKASLQMEALQYLRMMEEQAEYDMDALQKANELVEEKEIEIQDLLDELEQYRSKYGYDLMNTNMNTIKSFDDEKSYILESLSTLEEKIHQLSNGVYDKSTESENHVEFSKLEHQVLDLKEKMEALQADLDLIRHICNTLHANEGIEFIQEIANQLQDLRSVIFQQLRIHPSTKPSLDGIKSSDLHCEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BXU6</accession>
    <name>A0AAD5BXU6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">M8C21_021624</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">AA19_3_7</strain>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the leguminous lectin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010398">
        <property type="protein sequence ID" value="KAI7731733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BXU6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002229">
        <property type="term" value="P:defense response to oomycetes"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06899">
        <property type="entry name" value="lectin_legume_LecRK_Arcelin_ConA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000168">
        <property type="entry name" value="L-type lectin-domain containing receptor kinase IX.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000240">
        <property type="entry name" value="Lectin-domain containing receptor kinase A4.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050528">
        <property type="entry name" value="L-type_Lectin-RKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001220">
        <property type="entry name" value="Legume_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27007">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00139">
        <property type="entry name" value="Lectin_legB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0430">Lectin</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="221"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="260"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="289"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="17">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAI7731733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="530" mass="59494" checksum="23823F4E0B1FE678" modified="2024-05-29" version="1" fragment="single">GAYISEDAIHVTPENKSGTVIGRVTYIERLHLWDKSSRGLASFSTNFSFVKLSDPTNLVRQDDGFIFFLAQSSSMIHGATDPQLDTAADDSADQFVWVEFDPYCNTWDREAINVNSQSSIKQAEWFNDVSSERECQVWINYDSASRNLSVSFTSFRDNRVEVGGVAHTVDLRDELPELVVFGFSAATGEFSDKNTVKSWIFNSSDLQFDANKGLSPHKLRLVVGLVVGKSILVTLLVILAYVLWKKKKKKKNNARATCDFAEKEKLGEGSFGGVYKGFLKDLRTYVAVKRVSKTSQQGIKEYASEVRIISRLRHRNLVQLTGWCHEKGELLLVYEYMENGSLDSHLFKANSLLTWGTRYQIAIDFNAKLGDFGLAKFVDHEKGSQTTSFAGTLGYMAPECVTTGKTSKQSDVFSFGVVALEIVCGRKSINRKAQEKQIWLPGWVWELYGTHTLLEAVDPRLSLEFDEDEVKRLMIVGLWCVHPDSRLRPSMRQVIQVLNSKASLPILPSKMPVASYFTTQRPSLCDGSST</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BYZ0</accession>
    <name>A0AAD5BYZ0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ubiquitin-like protease family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_010072</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010260">
        <property type="protein sequence ID" value="KAI7732333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BYZ0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.395.10">
        <property type="entry name" value="Adenoviral Proteinase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7732333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="166" mass="19384" checksum="B6F1DB8EF933CB00" modified="2024-05-29" version="1" fragment="single">MAEKRTVSENNVSTYLFTGNGNLRDALFLISDVILVSRVKLESLIPGVEIHEDVINVWSHILNKEEKKKSRESDYRLFCTAPMIEKDTYDKDEQARVDEFTKNMTDVLRKENLKTIKKFDLVFVPIIQSKHYYLMCFNIKEPKTILIDNIASGNVEDMYNGWPEKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-04-02" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BZT0</accession>
    <name>A0AAD5BZT0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Exocyst component Exo84 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_001982</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the EXO84 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010214">
        <property type="protein sequence ID" value="KAI7732452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1210:FF:000002">
        <property type="entry name" value="Exocyst complex component EXO84B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1220:FF:000001">
        <property type="entry name" value="Exocyst complex component EXO84B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1220">
        <property type="entry name" value="Exo84p, C-terminal helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1210">
        <property type="entry name" value="Exo84p, N-terminal helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016159">
        <property type="entry name" value="Cullin_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033961">
        <property type="entry name" value="Exo84"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032403">
        <property type="entry name" value="Exo84_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042561">
        <property type="entry name" value="Exo84_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042560">
        <property type="entry name" value="Exo84_C_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21426">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21426:SF12">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16528">
        <property type="entry name" value="Exo84_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08700">
        <property type="entry name" value="VPS51_Exo84_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74788">
        <property type="entry name" value="Cullin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst component Exo84 C-terminal" evidence="6">
        <location>
            <begin position="164"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="562"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="732"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="565"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="743"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF16528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7732452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="783" mass="85973" checksum="3C3A1C37CCEE5215" modified="2024-05-29" version="1" fragment="single">IDTITAGGFPAKPDAAVDPSRGNNTPAKSNSKETGPKLEENLIIFKSDTFDADAFVNSKCNSLNEKEIRQLCSYLLDLKKASAEEMRRSVYANYTAFIRTSKEISDLEGELLSIRNLLSTQATLIHGLAEGIHVESLSVTVPGGSLSNGVSADMDRDPSDLENWLIELPDILDVLLAERRVEEALATLDEGEHVASEAKRKQSLSPALLASLQTAITECRQKLANQLADAASQPTTRGGELRAAISALKKLGDGPRAHSLLLQAYSLRLQSDMQNLRPSSTSYGGAYTAAVSQLVFSVIAQTATDANAIFGKEPTYSSELVMWATKQTEDFALLVKRHALASSAAAGGLRAAAECVQIALGHCSLLEARGLTLCPVLLRLFRPSVEQALDANLKRIEESTAALAAADDWDLSSSPTVTRLSGRPSSTSLNTITHQHKLSSSAQRFNSMVQDFFEDVGPLLSMQLGGKMLEGLFHVFNSYVSMLIRALPGSMEEEANYEGSGXXXXXXALPGSMEEEANYEGSGNTIVRMAETESQQMALLANASLLADELLPRAAMKLTPIMYNDDPRGRPSDRQNRNPEQREWKRRLASIVDRLKDSFCRQHALDLIFTEDGDTHLASEMYINMDESVEEIEWFPSPIFQELYAKLYRMSAIAAEMFAGRERFSTLLLMRLTETVILWLSEDQTFWEDILEGPRPLGPLGLQQFYLDMNVLPEDEWFGDICQDAMDKLSGKPRLANGERDPSSPTASVSAQSISSIRSHGSSYVVTTLEILSVLQYREHLSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C0K1</accession>
    <name>A0AAD5C0K1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">phosphopyruvate hydratase</fullName>
            <ecNumber evidence="3">4.2.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_013294</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="8">Mg(2+) is required for catalysis and for stabilizing the dimer.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the enolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.11" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010010077">
        <property type="protein sequence ID" value="KAI7733141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000015">
        <property type="term" value="C:phosphopyruvate hydratase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004634">
        <property type="term" value="F:phosphopyruvate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03313">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.390.10:FF:000001">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.120">
        <property type="entry name" value="Enolase-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.10">
        <property type="entry name" value="Enolase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00318">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000941">
        <property type="entry name" value="Enolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036849">
        <property type="entry name" value="Enolase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020810">
        <property type="entry name" value="Enolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020809">
        <property type="entry name" value="Enolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020811">
        <property type="entry name" value="Enolase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902:SF46">
        <property type="entry name" value="ENOLASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00113">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001400">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00148">
        <property type="entry name" value="ENOLASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01192">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01193">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
        <property type="entry name" value="Enolase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="Enolase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00164">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enolase N-terminal" evidence="10">
        <location>
            <begin position="4"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="Enolase C-terminal TIM barrel" evidence="9">
        <location>
            <begin position="118"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="154"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="292"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="267"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001400-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001400-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7733141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="384" mass="41368" checksum="6FCC59479F6355CC" modified="2024-05-29" version="1">MATIKSVKARQIFDSRGNPTVEVDVTLSDGTLARAAVPSGASTGIYEALELRDGGSDYLGKGVLKAVENVNSIIGPALIGKNPTEQTKIDNFMVQELDGTVNEWGWCKQKLGANAILAVSLAVCKAGASVKKIPLYKSVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKTAISKAGYSGKVVIGMDVAASEFYEGKDKTYDLNFKEENNDGSQKISGDSLKNVYKSXXXXXXXXXXXXXXDQDDWEHYAKMTAEIGEQVQIVGDDLLVTNPKRVEKAINEKTCNALLLKVNQIGSVTESIEAVKMSKRAGWGVMASHRSGETEDTFIADLSVGLATGQIKTGAPCRSERLAKYNQLLRIEEELGAAAVYAGSKFRAPVEPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C1G4</accession>
    <name>A0AAD5C1G4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Exostosin GT47 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_021266</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 47 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010254">
        <property type="protein sequence ID" value="KAI7732349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C1G4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004263">
        <property type="entry name" value="Exostosin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040911">
        <property type="entry name" value="Exostosin_GT47"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11062:SF217">
        <property type="entry name" value="EXOSTOSIN FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11062">
        <property type="entry name" value="EXOSTOSIN HEPARAN SULFATE GLYCOSYLTRANSFERASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03016">
        <property type="entry name" value="Exostosin_GT47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="Exostosin GT47" evidence="7">
        <location>
            <begin position="179"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7732349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="510" mass="58764" checksum="2996DBF8B1CF0C54" modified="2024-05-29" version="1">MLICRSNNLSSTLISVTLLAIVIVVAVSYASNGLSLLSLPASPLSWRWPSSTLSFPSSTNLDHSPPPAPPRLNPKTALYSMFNCDSDSCKKQNGKMNREVNKLIKELKDETMLRRNKKRDKKLEKVEAELAGARVLMRDAATRKHNNSSSSPVDDPDYVPNGNIYRNPYLFHRSYLLMEKLLKVYIYEEGEPPLFHKGPTFDIYSTEGVFLNLIEKDPHFRIFDPDRAHVFFLPFSVTMILNYLFDPIIRDKAVLERVIGDYVHIVSSRYPYWNQSLGSNHFMIACHDWGPRASWSVHSLYFTSIRLLCNANTSEYFNPRKDASIPQINLHRDEPTMVPGGLPASNRTTLAFFAGNEFHGKIRPLVFQYWKDKDEDILVYDRVPKNVSYHEMMHTSRFCLCPSGYEVASPRIVEAIYAECVPVLISQNYVLPFSDVLNWDAFSVRVPVSEIPNLKRILLGISDDEYTRLHENVKRAQRHFLVNNPAKRYDIYHMILHSLWLRRLNLNIYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C1V8</accession>
    <name>A0AAD5C1V8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein LURP-one-related 15-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_017001</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LOR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010026">
        <property type="protein sequence ID" value="KAI7733334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C1V8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.200">
        <property type="entry name" value="LURP1-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007612">
        <property type="entry name" value="LOR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038595">
        <property type="entry name" value="LOR_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025659">
        <property type="entry name" value="Tubby-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31087">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31087:SF122">
        <property type="entry name" value="TUBBY-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04525">
        <property type="entry name" value="LOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54518">
        <property type="entry name" value="Tubby C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="22"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7733334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="223" mass="24610" checksum="00715F6503BC01E9" modified="2024-05-29" version="1">MMAQPSNAPESPPVSVISPQFIVPYQFNIIVYSFSIGNFVITDMNHRILIMVKPCDTTLHSQRLLFDTNETSIALLREKSMSAHDRWNVFRGESNADSDMIFSTVSSHMIQFKTHLNVFLANKASRKDKFDFKIKGSWSKRNCTIYMGDSSTIIAQMHKNDGNHTLFKDKFTVTIYPNVDYSFVVALIAIVDAMENPISSGVVEAAGGGVGGAAGEAVGSFVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C2C5</accession>
    <name>A0AAD5C2C5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_000945</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009939">
        <property type="protein sequence ID" value="KAI7733589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C2C5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7733589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="70" mass="8120" checksum="1BCCEFA353CB797D" modified="2024-05-29" version="1">MYCLYSQVPSHSPLFFFPHVSFPALSRSTTCLSTHTLSPPPPPPPSPLFRLQSLRFRKQYLWLRSELKDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C2K4</accession>
    <name>A0AAD5C2K4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Actin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_005584</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the actin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009786">
        <property type="protein sequence ID" value="KAI7734191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C2K4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000148">
        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000205">
        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000047">
        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020902">
        <property type="entry name" value="Actin/actin-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004001">
        <property type="entry name" value="Actin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00190">
        <property type="entry name" value="ACTIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00432">
        <property type="entry name" value="ACTINS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01132">
        <property type="entry name" value="ACTINS_ACT_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7734191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="392" mass="43373" checksum="29CA8ED674D823F7" modified="2024-05-29" version="1">MDEFENHNPPVVLDSGTAVMKAGFTGDDSPRTIPSIVGKPHHTGVHVGSGRKDVYVGDDAQYRRGMLTLEYPMERGIVKNWDDMEKIWHSTFNNVLRVLSEEHPVLLTEAALNPKANREKMTEIMFEAFNVPAMYVANPAILSLYANGRTTGIVLDSGEGVSQVVPVFEGYGIPKSILRLDLAGADITDLLMMFLKGRGYELTTSADRDIVRDIKETLAYVALDYEQESKSAKNSSSDNKDYKLPDGHVITIGSERFRCAEVLFQPQLIEMEAAGIHETTYNGIMKCDPDLWEYLYGSIVLSGGSTMFPGMAERMRKEITTLAPSNMKIEVVEAPERNNSAWIGGSILSSLSTFQQVTSSTTASDDWVGLVMWISKADYNESGPAIVLEKCY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-06-18" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C2V0</accession>
    <name>A0AAD5C2V0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_017046</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009756">
        <property type="protein sequence ID" value="KAI7734341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03233">
        <property type="entry name" value="ABCG_PDR_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03232">
        <property type="entry name" value="ABCG_PDR_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000839">
        <property type="entry name" value="ABC transporter G family member 32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000059">
        <property type="entry name" value="ABC transporter G family member 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029481">
        <property type="entry name" value="ABC_trans_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034001">
        <property type="entry name" value="ABCG_PDR_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034003">
        <property type="entry name" value="ABCG_PDR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013581">
        <property type="entry name" value="PDR_assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48040:SF66">
        <property type="entry name" value="PLANT PDR ABC TRANSPORTER ASSOCIATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48040">
        <property type="entry name" value="PLEIOTROPIC DRUG RESISTANCE PROTEIN 1-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19055">
        <property type="entry name" value="ABC2_membrane_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14510">
        <property type="entry name" value="ABC_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08370">
        <property type="entry name" value="PDR_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="525"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="564"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="607"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="641"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="667"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="752"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1194"/>
            <end position="1212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1224"/>
            <end position="1240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1280"/>
            <end position="1300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1307"/>
            <end position="1325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1337"/>
            <end position="1356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1368"/>
            <end position="1387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1419"/>
            <end position="1441"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="176"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="852"/>
            <end position="1101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7734341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="1450" mass="163533" checksum="E4513608914F1F9A" modified="2024-05-29" version="1">MDDDRKMSISQSLGRSISRGISRAAGSWRMDDVFAPGGGGSHDGRSSRHSMEDEEALRWAALEKLPTYNRLRTTIFKSYIPADQQQQVPTEQMLLDVRDLDPQARQSFIDQIFKVAEEDNEIFLRKFRDRVDKVGISLPTVEVRYQNLSVEADCHVGDRALPTLPNTARNIAESLLASIGISFSEKAKLRILKNISGIIKPARMTLLLGPPSSGKTTLLLALAGRLDPSLRVEGEITYNGHKLSEFEPRRTAAYISQNDIHAGEMTVKETLDFSARCQGIGSRLEMLTELARREKEAGIIPEAEVDLFMKATAIEGDDSNQMRRGISGGQKKRVTTGEMLVGPAKTLFMDEISTGLDSSTTFQIVKCMQQVAHLTDSTIFMSLLQPAPETFDLFDDIILLSEGQIVYQGPREHTVEFFESCGFKCPERKGTADFLQEVTSKKDQEQYWADRNKAYRYIPVSEFCERFKSFHVGEKLRQELSVPYDKANSHKAALVYKKYLVPKMELLKASWDKEVLLMKRNAPVFVFKTIQILFMSFICTTLYLRTTMHQRNEIDGSLYVGALLNSMLINMFNGLADLSLIVMRLPVVYKQRDLMFHPPWAFTLPAFLLRIPISMIESVMWTVILYWGVDLAPDAGRFFKHLLLVFLIQNVAAGLFRLIAGVCKTMNIANTGGSVVLLLIFLLGGFILPKDSIPDWWQWAYWLSPLSYIFKSITINEFLDPRWTDQRSSDNSTNLGFAVLKNMNVPTTESSYWIGAAALLGFVFLFNILFTVALMYLEAPGKPQAIISKEEAAAMDGHATEGASSKGNKEMRQIRSAITDANGASKKGMVLPFIPLAMSFDDMNYYVDMPGVKSYDYLLKQEMREQGVTETRLQLLRDVTGAFRPGVLTALMGVSGAGKTTLMDVLAGRKTGGYIEGDIRISGFPKKQETFARISGYCEQTDIHTPTITVHESLIYSAFLRLSKEVSKEDKMLFVSEVMELVELDNIKNAIVGLPGVSGLSTEQRKRLTIAVELVANPSIIFMDEPTSGLDARAAAIVMRAVRNTVDTGRTVVCTIHQPSIDIFESFDELLLLKRGGQVIYSGPLGRHSQQIIDYFEAVNGVPKIPEKYNPATWMLEVSSGAAEMRLGIDFAEHFTASSLHQRNKALVKELSVPPPGATDLHFDTQYAQSSWGQFKSCIWKMWWSYWRNPDYNLVRFCFTLLCALMVGTVFWKIGNKKSSSNDLYTVIGAMYSAVFFVGINNSQTVQPVVATERTVFYRERAAGMYSSLPYAMAQVFVEIPYVLLQTIYYTAIVYTMVSFEWTAAKFFWSFFINFFSFLYFTYYGMLTVSVTPNEQVAAIFAAGFYLLFNIFSGFYIPRPKIPGWWIWYYWICPMAWTVYGCIVSQYHDANHQINVPGMSPSPTMTAYIKDYYGFELDFMGPVAAVLVGFCVFFALLYATFLRTLNFQMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C3H4</accession>
    <name>A0AAD5C3H4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Elongator complex protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_032107</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="3">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the WD repeat ELP2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009661">
        <property type="protein sequence ID" value="KAI7734688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C3H4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033588">
        <property type="term" value="C:elongator holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037289">
        <property type="entry name" value="Elp2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44111">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44111:SF1">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="7 11" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="216"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="550"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="697"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7734688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="697" mass="77034" checksum="8814FFE052405D6E" modified="2024-05-29" version="1" fragment="single">MVENRAVDVDRVFIGAGCNRIVNNVSWGACGLISFGSQNAVSIFCPQTAKILTTLPGHKASVNCTHWLPSSKFAFKATGWEKHYLLSGDAEGVIILWEFSLAENKWKYVSQLPKSHKKGVTCITAIVTSQTEAIFASTSSDCVVNVWKVDLPASGGDCTVTCLDTLSAGIKPMVTLSLAELPGSTTRLILAMGGLDNKIHLYVGETKGQFARTCELKGHTDWIRSLDFSLPVTINEEPHNLLLVSSSQDKGIRIWKMALCDSSSNFDKKKAANTLAYYIKGPVFIAGSFSYQVTLESLVIGHEDWVYSVEWQPPSLLENSSCFQPQSILSASIDKTMMIWKPERTTGIWVNVVTVGELSHSALGFYGGHWSPNGDSILAHGYGGSFHLWKNVGNEYDNWKPQKVPSGHFAAVTDIAWGKYGEYLMSVSDDQTTRIFASWFDEAKLKEDCDTWYEIARPQVHGHDINCLAIIQGKGNHRFVSGADEKVARVFEAPLSFLKTLSHATSHQHDDLQVDVQVLGANMSALGLSQKPIYAQEGSDQISYQLITRQEAHKRIVWACSWNPSGHQFATGSRDKTVKIWEVQKEKSNVQLLMTLPAFKSSVTALSWTGSHRQNDDGVLAIGLETGLIELWGLSLNKHEQDSSFSGPKAHLLIQFDPFMCHVSSVNRLAWRNTEKNDESDDMQLASCGADNCVRIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C527</accession>
    <name>A0AAD5C527_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_030852</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009535">
        <property type="protein sequence ID" value="KAI7735195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C527"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="138"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="280"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="156"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="188"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="202"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="234"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7735195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="383" mass="42015" checksum="E6E5793D796C4B0F" modified="2024-05-29" version="1">MSSDGDEQETDYQIRGPPGGLYLGDRGRQGPPWEAGSSGGWVGLYTNHYGTYNPPGPHYSFGTDFCGPAWVGPKETDFSKKNGPRFEGQRVNNFVEDCPETQRERVPEGYGLKVVNADVTGRVRKKIQYDIVKGLNYSGEKGVKTGQDGSFKESSNKPQAQVVNKGSNEGRPKTRRGKRKANKADVNGTKNSCTGSLGKQVVKTDQDDKVADNTVAGEEDIKDDNTDVLLQDSSKPDQWAKSESDSWGKAIENETNFILRSSDKSLDWSKLVEAKVASQHGSGALWGKKGDNKSQAKAVTDGSNEKGNVENQKPGSWWSKEEGCGSKRKHYDSLVGGPNDGPISSGLFTTSKRRADMFTTDEQEIHLNEIEPIVQSIRRIMNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C6N4</accession>
    <name>A0AAD5C6N4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Small nuclear ribonucleoprotein-associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_022402</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the snRNP SmB/SmN family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009398">
        <property type="protein sequence ID" value="KAI7735990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C6N4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005687">
        <property type="term" value="C:U4 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070990">
        <property type="term" value="F:snRNP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01717">
        <property type="entry name" value="Sm_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000004">
        <property type="entry name" value="Small nuclear ribonucleoprotein-associated proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017131">
        <property type="entry name" value="snRNP-assoc_SmB/SmN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050914">
        <property type="entry name" value="snRNP_SmB/NAA38-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10701:SF0">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN-ASSOCIATED PROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10701">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN-ASSOCIATED PROTEIN B AND N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037187">
        <property type="entry name" value="snRNP_SmB/SmN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="9 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="10 11" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="7 11" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Sm" evidence="13">
        <location>
            <begin position="4"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="159"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="159"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="174"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="213"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR037187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7735990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="274" mass="29120" checksum="D7BDA7929BC1B384" modified="2024-05-29" version="1">MSMSKGSKMLQFINYRMRITIQDGRQLVGKFMAFDRHMNLVIGDCEEFRKLPPAKGSKKHEEREDRRTLGLVLLRGEEVISMTVEGPPPPDENRAKAVGAAALAGPGLGRAAGRGIPTAPLVQAQPGLAGPVRGIGGPAPGMMQPQISRPPVPNMAAPPVNYPVIRPGQMPYPGQGPPQMPRGPPPQMPPQFAQRPPGQYPVPPPGQYGQRPMAPPPQMMRGPVPPGGAPRPGMPGPPPPARPGMPPPPGGQMQYPGPPRPGMPPPPNSQQQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C6Q5</accession>
    <name>A0AAD5C6Q5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_019804</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Mediator complex subunit 23 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009594">
        <property type="protein sequence ID" value="KAI7735006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C6Q5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010628">
        <property type="term" value="P:positive regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021629">
        <property type="entry name" value="Mediator_Med23"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12691">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12691:SF10">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7735006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="315" mass="35507" checksum="884546C8369C70A6" modified="2024-05-29" version="1" fragment="single">MSDTGDQNHHHSTASRTTLFHPYRPAIIDLFNLYLGDHEQLYTVVESVLVSLVIQCCNHGPRSKFILFAIRSLYSIGYIYWDSFLPSLLSSVTSAEMSVNIFSTTSPQSDVATSAAGPNSSNIQSPNPVSSLVSANEQSCTAISSLRKLCCEIFISAMELNLKPSTYAAIFNQILSWLINWDSSQQGRDEVDGVKLYKLDKALFEWLHKCLGLVRMLVEDNKCRVPFYELLRSRLHFLENLPDDVALFTLVFEVHRRRDMMALHMQMLDQHLHCPTFGNQRLLNQAAVNTLSESNTNLIHSPITYPSVLGEPLHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C7G9</accession>
    <name>A0AAD5C7G9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glycoside hydrolase family 38 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_012082</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009258">
        <property type="protein sequence ID" value="KAI7736477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C7G9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004559">
        <property type="term" value="F:alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006013">
        <property type="term" value="P:mannose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.110.10">
        <property type="entry name" value="Glycoside hydrolase 38, N terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000602">
        <property type="entry name" value="Glyco_hydro_38_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027291">
        <property type="entry name" value="Glyco_hydro_38_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050843">
        <property type="entry name" value="Glycosyl_Hydrlase_38"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11607">
        <property type="entry name" value="ALPHA-MANNOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11607:SF3">
        <property type="entry name" value="LYSOSOMAL ALPHA-MANNOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01074">
        <property type="entry name" value="Glyco_hydro_38N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042133071" description="Glycoside hydrolase family 38 N-terminal domain-containing protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 38 N-terminal" evidence="2">
        <location>
            <begin position="37"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7736477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="123" mass="14218" checksum="046C65C9C7A96362" modified="2024-05-29" version="1" fragment="single">MDFRVLVMLILSVVYVCVESKYMVYDTAQGVVKGKVNVHLVAHTHDDVGWLKTVDQYYVGSNNSIQGACVRNVLDSVVQALLKDKNRKFIYVEQAFFQRWWRDQSEETQDIVKRLVSSGQLEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C7M8</accession>
    <name>A0AAD5C7M8_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_021517</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 32 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009183">
        <property type="protein sequence ID" value="KAI7736833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18624">
        <property type="entry name" value="GH32_Fruct1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.560:FF:000002">
        <property type="entry name" value="Beta-fructofuranosidase, insoluble isoenzyme CWINV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.560">
        <property type="entry name" value="Exo-inulinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.115.10.20">
        <property type="entry name" value="Glycosyl hydrolase domain, family 43"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050551">
        <property type="entry name" value="Fructan_Metab_Enzymes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001362">
        <property type="entry name" value="Glyco_hydro_32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013189">
        <property type="entry name" value="Glyco_hydro_32_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013148">
        <property type="entry name" value="Glyco_hydro_32_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023296">
        <property type="entry name" value="Glyco_hydro_beta-prop_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31953">
        <property type="entry name" value="BETA-FRUCTOFURANOSIDASE, INSOLUBLE ISOENZYME CWINV1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08244">
        <property type="entry name" value="Glyco_hydro_32C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00251">
        <property type="entry name" value="Glyco_hydro_32N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00640">
        <property type="entry name" value="Glyco_32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75005">
        <property type="entry name" value="Arabinanase/levansucrase/invertase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyl hydrolase family 32 N-terminal" evidence="5">
        <location>
            <begin position="4"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 32 N-terminal" evidence="5">
        <location>
            <begin position="276"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 32 C-terminal" evidence="6">
        <location>
            <begin position="342"/>
            <end position="532"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7736833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="543" mass="61308" checksum="05AFD927A61D5643" modified="2024-05-29" version="1">MYVGPLYHMGWYHLFYQYNPYSAIWGNITWGHAVSKDLINWFKLPLAMVPDHWYDQKGVMTGSATILPDGRIMMYYTGNAQDLSQLQCLAFAVNSSDPLLVEWVKYKGNPILGPPPGVGRKDFRDPSTLWLGPDGKYRMVMGSKHNNTIGCALIYHTTNFTHFELLDEVLHAVPHTGMWECVDLYPVSTTKTYGLDMSSYEADAKYVLKQSGDEDRHDWYAIGAYDVVKDKWYPDDPEIDVGIGLRYDYGKFYASKTFYDPSKKRXXXXXXXXXXYGKFYASKTFYDPSKKRRVLWGYVGEMDLKRDDLKKGWANMLNIPRTVVLDPKTQSNLIQWPVEEAETLRSKKYDEFKDVELAPGSLVPLDIGSTTQLDISASFEVDEALLEATLEADTVFNCTTSNGSAMRGALGPFGVVVLADAGLSEQTPIYFYIAKNTDGTSRTYFCADESRSSEILGIRKFVYGSTVPVLHGENYNMRLLVDHSIVESFAQGGRTVITSRVYPTKAIYDAAKVFLFNNGTGITVKASLKIWKMGAAKLKQFPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C870</accession>
    <name>A0AAD5C870_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_012095</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009258">
        <property type="protein sequence ID" value="KAI7736490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C870"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34044">
        <property type="entry name" value="NUCLEAR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34044:SF1">
        <property type="entry name" value="NUCLEAR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7736490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="304" mass="33333" checksum="2E719ED2892E60E2" modified="2024-05-29" version="1">MANVTTTLFSSKPYSLGNLNFKLPSTTSKHRTITSMVSVPTKVIPSIIVGGDISLGKALKQMGTGQDLVLKTGEPVPVDFPGPILVCTKNDDLDAVLESTPQSRWSDLVFFQDGMLMPWFESKGLSDPNQVLPFFDVSRPGKPPVDGITDVTPEGLTTAFGKWAPAVASRLHAGGLSCKILEKQAFQNRMIERLLWISAFMLTGARVPGGTMGDVASENHLYEVVMLMGEMAQATYEETGLAPEVHFETRLLAYSKAVSHIPAVVEDFKWRNGWFYSISQKAIAQGKEDPMSLHTRFLKEVNII</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C8E6</accession>
    <name>A0AAD5C8E6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Fungal lipase-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_008477</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009179">
        <property type="protein sequence ID" value="KAI7736845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C8E6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00519">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002921">
        <property type="entry name" value="Fungal_lipase-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044819">
        <property type="entry name" value="OBL-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46086">
        <property type="entry name" value="ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46086:SF19">
        <property type="entry name" value="TRIACYLGLYCEROL LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01764">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fungal lipase-type" evidence="2">
        <location>
            <begin position="212"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7736845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="454" mass="51738" checksum="5381C0016B93D9F9" modified="2024-05-29" version="1">MAIEGTSTRPCDNSFCEDRLLLSPKDVGLWDLMKLLFSKNIGNRKFIDCPKGTTEESFSRRFVIFISIVAQKVLHLVNKPLNWVGSAVEFMPNFMDVNGGFFKLLGNIASGNMPVLPNNESPDYLTAIGLWDIRRDLDNRIKHDDPRYTSALAIMAAKSAYENEAYIKETVEKHWKMEFLEFFNCWNDYEEDYTTQGFMWSDKTGNSELIGVSFRGTSPYNAKDWSSDVDLSWYKLPSIGKVHAGFLKALGLRKSQGWPNDIQSENSEKPYAYYTIRKKLKERLQNNPNAKFLVTGHSLGAALSILFPAVLAHHNENDLLSKLEGVYTFGQPRVGDREFGEYMTKVLVEHGLRYRSFYKGQMLPEEPNKNYFSILAIIPMFANAFWELLRSFIIYYQNGPEYCETFTCRGWRVIGLLIAGLPAHGPQDYVNLTRLGSPELFATSSADQLTNGVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C8R3</accession>
    <name>A0AAD5C8R3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">inositol-1,3,4-trisphosphate 5/6-kinase</fullName>
            <ecNumber evidence="4">2.7.1.159</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_009021</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ITPK1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.159" evidence="4"/>
    <dbReference type="EMBL" id="JAMZMK010009096">
        <property type="protein sequence ID" value="KAI7737182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C8R3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052726">
        <property type="term" value="F:inositol-1,3,4-trisphosphate 5-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052725">
        <property type="term" value="F:inositol-1,3,4-trisphosphate 6-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047325">
        <property type="term" value="F:inositol-3,4,5,6-tetrakisphosphate 1-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032957">
        <property type="term" value="P:inositol trisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000047">
        <property type="entry name" value="Inositol-tetrakisphosphate 1-kinase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008656">
        <property type="entry name" value="Inositol_tetrakis-P_1-kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040464">
        <property type="entry name" value="InsP(3)kin_ATP-grasp"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14217">
        <property type="entry name" value="INOSITOL-TETRAKISPHOSPHATE 1-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14217:SF1">
        <property type="entry name" value="INOSITOL-TETRAKISPHOSPHATE 1-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05770">
        <property type="entry name" value="Ins134_P3_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038163">
        <property type="entry name" value="ITPK_uncN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Inositol 1,3,4-trisphosphate 5/6-kinase ATP-grasp" evidence="11">
        <location>
            <begin position="277"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF05770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7737182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="477" mass="53081" checksum="00A9199B141BE04B" modified="2024-05-29" version="1">MAGGGGVRGIVLDSSVILNSDTLCPGADYLLRKLRYSNIPTGLSYAADLSEAKVSLLEKLACEYSLERFIYNPSCMDDTVNAVSLAWKNKGATILHVVSNYNEGIVPKSINSGWINVVVNVDGDSASKNPNGILIEKLEELPLTICELNRKPSREEDFAKRGAFPLNPTENGLIFMPLTFDLPMLYQLKKVDIVIHKATDEISSIERSNSCDGSSNIIYTTRMQELQRYIGDLPDCCVIDPFDNIFPVVDRLKIQEILLGLTNLKTESQHKIRGAYFLKVDNFEDVQLEQRLHEAKLSLPSIVKPQVACGVAEAHSMAIVFKASDFVGLSVPLPAVVQEYVDHSSTLFKMYVLGEKVFYAVKNSTPNADILKNSSEKNGFKPLLFDSLKSLPIDGSKMKEQPELDIQLVTDAATYLRRVLDITIFGFDVVLQEGTRDHVIVDVNYLPSFKEVPNEIAIPAFWDAIRMKFHTRKGVLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CAU7</accession>
    <name>A0AAD5CAU7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein RER1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_032889</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RER1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008799">
        <property type="protein sequence ID" value="KAI7738541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CAU7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006621">
        <property type="term" value="P:protein retention in ER lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004932">
        <property type="entry name" value="Rer1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10743">
        <property type="entry name" value="PROTEIN RER1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10743:SF28">
        <property type="entry name" value="PROTEIN RER1C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03248">
        <property type="entry name" value="Rer1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="38"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7738541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="121" mass="13989" checksum="77E04FD15BEA9577" modified="2024-05-29" version="1">MNVGGIDTDTDLKPSPLSQHTHNLSRKYQHLLDKSTPLVLHRWIGFSLVILIYALRVYFVQGFYIVTYGLGIYILQLFLAFLSPQVDPELHNDDGPSLPTLGSEEFRPFVRRLPEFKFWLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CB04</accession>
    <name>A0AAD5CB04_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">inositol-1,3,4-trisphosphate 5/6-kinase</fullName>
            <ecNumber evidence="4">2.7.1.159</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_009020</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ITPK1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.159" evidence="4"/>
    <dbReference type="EMBL" id="JAMZMK010009096">
        <property type="protein sequence ID" value="KAI7737181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CB04"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052726">
        <property type="term" value="F:inositol-1,3,4-trisphosphate 5-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052725">
        <property type="term" value="F:inositol-1,3,4-trisphosphate 6-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047325">
        <property type="term" value="F:inositol-3,4,5,6-tetrakisphosphate 1-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032957">
        <property type="term" value="P:inositol trisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000047">
        <property type="entry name" value="Inositol-tetrakisphosphate 1-kinase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008656">
        <property type="entry name" value="Inositol_tetrakis-P_1-kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040464">
        <property type="entry name" value="InsP(3)kin_ATP-grasp"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14217">
        <property type="entry name" value="INOSITOL-TETRAKISPHOSPHATE 1-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14217:SF1">
        <property type="entry name" value="INOSITOL-TETRAKISPHOSPHATE 1-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05770">
        <property type="entry name" value="Ins134_P3_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Inositol 1,3,4-trisphosphate 5/6-kinase ATP-grasp" evidence="11">
        <location>
            <begin position="193"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF05770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7737181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="393" mass="43975" checksum="191C711389FEB175" modified="2024-05-29" version="1">MDDTVNAVSLAWKNKGATILHVVSNCNERLIPKSINSGWINVVVNVDGDSASKNPSGILIEKLEELPLTICELNRKRGAFPLNPTENGLIFMPLTFDLPMLYQLKKVDIVIHKATDEISSIERSNSCDGSSNIIYTTRMQELQRYIGDLPDCCVIDPFDNIFPVVDRLKIQEILLGLANLKTESQHKIRGAYFLKVDNFEDVQLEQRLHEAKLSLPSIVKPQVACGVAEAHSMAIVFKASDFVGLSVPLPAVVQEYVDHSSTLFKMYVLGEKVFYAVKNSTPNADILKNSSEKNGFKPLLFDSLKSLPIDGSKMKEQPELDIQLVTDAATYLRRVLDLTIFGFDVVLQEGTRDHVIVDVNYLPSFKEVPNEIAIPAFWDAIRMKFHTRKGVLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CBA2</accession>
    <name>A0AAD5CBA2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Beta-fructofuranosidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_030794</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the glycosyl hydrolase 32 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008750">
        <property type="protein sequence ID" value="KAI7738692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CBA2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18624">
        <property type="entry name" value="GH32_Fruct1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.115.10.20:FF:000001">
        <property type="entry name" value="Beta-fructofuranosidase, insoluble isoenzyme CWINV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.560:FF:000002">
        <property type="entry name" value="Beta-fructofuranosidase, insoluble isoenzyme CWINV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.560">
        <property type="entry name" value="Exo-inulinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.115.10.20">
        <property type="entry name" value="Glycosyl hydrolase domain, family 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050551">
        <property type="entry name" value="Fructan_Metab_Enzymes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001362">
        <property type="entry name" value="Glyco_hydro_32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013189">
        <property type="entry name" value="Glyco_hydro_32_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013148">
        <property type="entry name" value="Glyco_hydro_32_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023296">
        <property type="entry name" value="Glyco_hydro_beta-prop_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31953">
        <property type="entry name" value="BETA-FRUCTOFURANOSIDASE, INSOLUBLE ISOENZYME CWINV1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08244">
        <property type="entry name" value="Glyco_hydro_32C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00251">
        <property type="entry name" value="Glyco_hydro_32N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00640">
        <property type="entry name" value="Glyco_32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75005">
        <property type="entry name" value="Arabinanase/levansucrase/invertase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0865">Zymogen</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="36"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 32 N-terminal" evidence="8">
        <location>
            <begin position="107"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 32 C-terminal" evidence="9">
        <location>
            <begin position="426"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="11"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7738692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="634" mass="71369" checksum="8F24EF0A13901F37" modified="2024-05-29" version="1">MASSTTTTPLIHHDDPENLHELTGSSAARRPSIAKAVFMTLVSVMVICALVAVVSNYSNQTREPRVASNHEGAATTFTTQLPKTDVIRVPGKLDASADVEWQRSAYHFQPDKNFISGPMYHMGWYHLFYQYNPESAIWGNITWGHSVSKDMINWFHLPFAMVPDHWYDIEGVMTGSATVLPNGQIIMLYTGNAYDLSQVQCLAYAVNSSDPLLIEWKKYEGNPILFPPPGVGYKDFRDPSTLWLGPDGEYRMVMGSKHNETIGCALIYHTTNFTHFELKEEVLHAVPHTGMWECVDLYPVSTTHTNGLDMVDNGPNVKYVLKQSGDEDRHDWYAIGSYDVVNDKWYPDDPENDVGIGLRYDFGKFYASKTFYDQHKKRRVLWGYVGETDPQKYDLSKGWANILNIPRTVVLDTKTKTNLIQWPIEETEKLRSKKYDEFKDVELRPGSLVPLDIGTTTQLDIVATFEVDQKMLESTLEADVLFNCTTSEGSVARGVLGPFGVVVLADAQRTEQLPVYFYIAKDIDGTSRTYFCADETRSSTDIDVGKWVYGSSVPVLPGEKYNMRLLVDHSIVEGFAQNGRTVVTSRVYPTKAIYGAAKLFLFNNATGISVKASMKIWKMAEAELNPYPLTGWTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CBB9</accession>
    <name>A0AAD5CBB9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_003494</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ARG7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008745">
        <property type="protein sequence ID" value="KAI7738722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CBB9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009733">
        <property type="term" value="P:response to auxin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003676">
        <property type="entry name" value="SAUR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31374">
        <property type="entry name" value="AUXIN-INDUCED PROTEIN-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31374:SF16">
        <property type="entry name" value="AUXIN-RESPONSIVE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02519">
        <property type="entry name" value="Auxin_inducible"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7738722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="134" mass="15432" checksum="EAFE861BE5E14FBC" modified="2024-05-29" version="1">MAKVKNISNGKKNNGILKLKFVLERLQKGLFLAKKRQESYSGQEMVPKDVKEGHFAVIASDDYVERRFVIPITYLRHPSFLRLLERAAEEYGFDHEGALMIPCRPSELEWMLEEQIGSQHGADWNSCKTMVESC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CEW5</accession>
    <name>A0AAD5CEW5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RRP12-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_031103</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RRP12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008490">
        <property type="protein sequence ID" value="KAI7740189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CEW5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012978">
        <property type="entry name" value="HEAT_RRP12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057860">
        <property type="entry name" value="HEAT_RRP12_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48412">
        <property type="entry name" value="ARM REPEAT SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48412:SF1">
        <property type="entry name" value="ARM REPEAT SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25772">
        <property type="entry name" value="HEAT_RRP12_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08161">
        <property type="entry name" value="RRP12_HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RRP12 N-terminal HEAT" evidence="4">
        <location>
            <begin position="17"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="RRP12 HEAT" evidence="3">
        <location>
            <begin position="150"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="786"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="8"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="786"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="822"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="857"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="891"/>
            <end position="906"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7740189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="906" mass="100011" checksum="B920AFB211C0AF00" modified="2024-05-29" version="1">MKSAVKTVDSRSVDGSKDENTSKGEHLEVLHMLNLLKFLVPCLPSKVISKVVIELQTAISASFSALTRHVFDVMDEILRFLEAGSTIPDTEKIVTILSTYISKKQNPVDTIFSAAALLDTFLTKFQIGDPTKWNDHYSLIIGSIAGLLTSESTAMKASNILKEMINHHIDVEILLSSETMLADENNVESKESRLLKSLCDALLKVISAHKGIPNEHILAVISALFLKLGKSSHIYMGSVLLKLAAFMTATSGNTYDVKHLQECMGSAVIAMGPEKLLDILPISLVANDQTCSNSWLIPILKEYVAGSSLGFFIENVVPLADSFQEESQKVKKSVSGEELQAYASGCWGLLPAFCRYPSDTHKKFQSLERLLIPCIRKDAFMLEDIAIAIQLLVKQNRSFLGAEQCDDEALKFPKLSYSKKAATKNIKALASCSEELLKAFTKVFFKVSKKKRVFVKDAIKCLTLITEPATIKKIFISSLKRLEQKVSADKVNAKRCLILDLASSVVGAASLDLIELIYNFIKQSLQEEDENIQREAYATLHKILEQSSAFCSSKFEELTDLVLGLKSPDNVTSLKWRLSCFRNLIIHSVEITLDGENTCGFRMLNEVIVTLKDGKEESRKVAYDVLLGISSTLQKTASTLEKGPYYEFVSMIMGYLSGSSPHIKSGAVSALSLVVYNDSKICTLMPDLVPSILELLHSKAIEVIKAVLGFLKVLVLSLQVSELQNFLSDILSGLLPWSSVSRHHFKSKVTVILEIMMRKCGSASVKSLVPAKYLDFVKDVLENRQGKTNSQETATTGTDAGLSETTTNSKPKFKSKTAGSFTKKDHSTETRKRKRDDKNCPDKPSKFSPRGNNNTTKDVKHSGFAKSSDHLSKGGFKRKNPSFKNDTSSGKRQKQWTKGSKKNASG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CFR0</accession>
    <name>A0AAD5CFR0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Prefoldin subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_030105</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the prefoldin subunit beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008374">
        <property type="protein sequence ID" value="KAI7740669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CFR0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009409">
        <property type="term" value="P:response to cold"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd23163">
        <property type="entry name" value="Prefoldin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.370:FF:000002">
        <property type="entry name" value="Prefoldin subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027235">
        <property type="entry name" value="PFD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002777">
        <property type="entry name" value="PFD_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13303">
        <property type="entry name" value="PREFOLDIN SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01920">
        <property type="entry name" value="Prefoldin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="121"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="86"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="121"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7740669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="146" mass="16295" checksum="3901E046D4469FF2" modified="2024-05-29" version="1">MAGVSGSEHKEPINEQEVAAKYGSIRAEMNQIYSKITELEMEVSEHSLVINAIQPLDPTRRCYRMIGGVLVERTIKEVLPAVQQNKEGIEVVIARLNEALERKKKELTDFETKYKIRIRKNDGESVDDNSKKEGSAQGVLVGPANA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CH18</accession>
    <name>A0AAD5CH18_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_003986</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008189">
        <property type="protein sequence ID" value="KAI7741472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CH18"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47344">
        <property type="entry name" value="RING ZINC FINGER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47344:SF1">
        <property type="entry name" value="RING ZINC FINGER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="11"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="169"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="176"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7741472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="218" mass="23728" checksum="150E575A95FBE4BF" modified="2024-05-29" version="1">MKDNEVLRALKASKQTKYPKRSLNGVDPALNDTCTPQDTTKEPDIHLGKTKRLRVPENINDSVPQPTKRASNDTSNLSKNLHKTSEHNGFPWHDCKDDDVVFLYESGHIAKDSSTPVYNPQEAAADFFFSNGLLDATNRHPSKWCKKGQNKAASGDLIAVGADGRAGTTKVLKSPNLPSLNSRGSSNSAKSRKLGAKASSLQSQGSLQINHFFSKVGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CKF4</accession>
    <name>A0AAD5CKF4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Epidermal patterning factor-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_000735</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Controls stomatal patterning.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the plant cysteine rich small secretory peptide family. Epidermal patterning factor subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007713">
        <property type="protein sequence ID" value="KAI7743536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CKF4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010052">
        <property type="term" value="P:guard cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039455">
        <property type="entry name" value="EPFL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33109:SF102">
        <property type="entry name" value="EPIDERMAL PATTERNING FACTOR-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33109">
        <property type="entry name" value="EPIDERMAL PATTERNING FACTOR-LIKE PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17181">
        <property type="entry name" value="EPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041770802" description="Epidermal patterning factor-like protein" evidence="7">
        <location>
            <begin position="29"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="79"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="86"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7743536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="127" mass="14105" checksum="7756A5707FBF596C" modified="2024-05-29" version="1">MAMNSLQSFTLSLLFTLQFLSLTSNCLLLHITPSTTTSTTTIRGLLYEEKARLGSTPPSCHNKCNLCHPCMAVQVPTMPGHNRVKPGQDRVKPTDYYDQPPSGGSNRYSNYKPLGWKCRCGGHLYNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CLQ2</accession>
    <name>A0AAD5CLQ2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_024735</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007557">
        <property type="protein sequence ID" value="KAI7744256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CLQ2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7744256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="68" mass="8059" checksum="466546975A0C8BA9" modified="2024-05-29" version="1" fragment="single">VIQSRRWSRGLRLRSELRGIDKQVSFWNSTNHPYLQQKKSIWVHHFTKTTIQDVSDSLYFVVNIAGKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CLU6</accession>
    <name>A0AAD5CLU6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">THH1/TOM1/TOM3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_019465</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the plant tobamovirus multiplication TOM1 protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007570">
        <property type="protein sequence ID" value="KAI7744218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CLU6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040226">
        <property type="entry name" value="THH1/TOM1/TOM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009457">
        <property type="entry name" value="THH1/TOM1/TOM3_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31142:SF43">
        <property type="entry name" value="THH1_TOM1_TOM3 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31142">
        <property type="entry name" value="TOBAMOVIRUS MULTIPLICATION PROTEIN 1-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06454">
        <property type="entry name" value="THH1_TOM1-3_dom"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="141"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="180"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="THH1/TOM1/TOM3" evidence="8">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="THH1/TOM1/TOM3" evidence="8">
        <location>
            <begin position="119"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF06454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7744218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="214" mass="25056" checksum="CAF21DDBA0139BB9" modified="2024-05-29" version="1" fragment="single">VQLVRIQMRVPEFGWTTQKVFHFMNFVVNGCKVLDMILLDLPGLLFFSTYTLLVLFWAEIYHQVFTFMAFCTLPYLQIIQARSLPTDKLRPAYFIINGIVYFLQQYFCCRGNCRTLLFRLFVMLRHFPVESRGRQKKLQEVGLVTGVCCTCFLVRCVMVAIAAFNKNADIHVLNHPILDFAYYMIVEILPSALVLFILRKLPPRRVSDQYHPIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CMQ9</accession>
    <name>A0AAD5CMQ9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">60S ribosomal protein L37a</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_011285</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL43 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007733">
        <property type="protein sequence ID" value="KAI7743434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CMQ9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.30:FF:000002">
        <property type="entry name" value="60S ribosomal protein L37a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00327">
        <property type="entry name" value="Ribosomal_eL43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011331">
        <property type="entry name" value="Ribosomal_eL37/eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002674">
        <property type="entry name" value="Ribosomal_eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00280">
        <property type="entry name" value="eL43_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48149">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L37A-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48149:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL43Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01780">
        <property type="entry name" value="Ribosomal_L37ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0934">Plastid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7743434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="106" mass="11909" checksum="CA75C4E8AC05E072" modified="2024-05-29" version="1">MLNKDCLFPCKLYTQTKRTKKAGIVGKYGTRYGASLRKQIKKMEVSQHSKFFCEFCGKYAVKRKAVGIWGCKDCGKVKAGGAYTLNTASAVTVRSTIRRLREQTES</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CNJ8</accession>
    <name>A0AAD5CNJ8_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">M8C21_007765</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">AA19_3_7</strain>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Voltage-dependent rectifying anion channel that facilitates the translocation between chloroplast and cytoplasm of phosphorylated carbohydrates such as triosephosphate, 3-phosphoglycerate and inorganic phosphate (Pi) depending of ATP to triosephosphate ratio in the plastidial intermembrane space; in high triosephosphate/ATP conditions (e.g. photosynthesis), export of triosphosphate from chloroplast (outward rectifying channels), but in high ATP/triosephosphate conditions (e.g. dark phase), import of phosphosolutes (inward rectifying channels).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast outer membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Plastid</location>
            <location evidence="2">Etioplast membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the plastid outer envelope porin OEP21 (TC 1.B.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007693">
        <property type="protein sequence ID" value="KAI7743751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CNJ8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009707">
        <property type="term" value="C:chloroplast outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034426">
        <property type="term" value="C:etioplast membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046930">
        <property type="term" value="C:pore complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015288">
        <property type="term" value="F:porin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008308">
        <property type="term" value="F:voltage-gated monoatomic anion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044070">
        <property type="term" value="P:regulation of monoatomic anion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034575">
        <property type="entry name" value="OEP21"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35993:SF3">
        <property type="entry name" value="OUTER ENVELOPE PORE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35993">
        <property type="entry name" value="OUTER ENVELOPE PORE PROTEIN 21B, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0934">Plastid</keyword>
    <keyword evidence="9" id="KW-1002">Plastid outer membrane</keyword>
    <keyword evidence="11" id="KW-0626">Porin</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1134">Transmembrane beta strand</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="17"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAI7743751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="192" mass="21765" checksum="7E9561C2D9B5CA12" modified="2024-05-29" version="1">METSVRYGGESSALRISNNNNNPPNSSPRVKYAGDVSSIKIHAKQKIQFDSDTLLQLNGELDTRTGAPTFLCALVRHFYPNLSASVGAGLQYDRNDKLVCTLRGKKAYPVASNRFVNVLLKGRCDIDKELNQPTPHGALELVWNILDFQKDQDVRLKVGYEIVDKIPYVQIRENNWTFNADINGRWNVRYHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CR18</accession>
    <name>A0AAD5CR18_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Terpene synthase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_005067</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007317">
        <property type="protein sequence ID" value="KAI7745250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CR18"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010333">
        <property type="term" value="F:terpene synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.130:FF:000001">
        <property type="entry name" value="Isoprene synthase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.130">
        <property type="entry name" value="Terpene synthase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001906">
        <property type="entry name" value="Terpene_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036965">
        <property type="entry name" value="Terpene_synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050148">
        <property type="entry name" value="Terpene_synthase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31225">
        <property type="entry name" value="OS04G0344100 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31225:SF235">
        <property type="entry name" value="TERPENOID CYCLASES_PROTEIN PRENYLTRANSFERASE ALPHA-ALPHA TOROID-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01397">
        <property type="entry name" value="Terpene_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Terpene synthase N-terminal" evidence="1">
        <location>
            <begin position="19"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7745250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="309" mass="35842" checksum="B606F6169B8E8A2F" modified="2024-05-29" version="1">MSLQKNFVIRPIANFPLEIWGDQFLVLNQPPVQDGVEQVIEDLKDELKKEILTTLEIPSQHTDMLKLIDAIQRLGIAYYFEDEINKALQHIFFAYGDKWTCTNTSLWFRLLRQEGYFVSSDLFNKYKDETGSFKESLKDDVHLLLELYEATNMRVPGEVILDEALAFTRTCLVDLANDPVLRNSNVSTFIQDALNQPLLKRLRRVEALRYIPFYEQQPMHNESLLKLAKLGFNLLQSLHKKELSQICNAWTELNGEFIACKDATLPIKMRVIDFARSMEVLYKNKDHFTNVGDELKNHIKSLLVDAIIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CRV9</accession>
    <name>A0AAD5CRV9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ATP-dependent RNA helicase</fullName>
            <ecNumber evidence="10">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">M8C21_029979</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">AA19_3_7</strain>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">RNA helicase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="domain">
        <text evidence="10">The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the DEAD box helicase family. DDX18/HAS1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="10"/>
    <dbReference type="EMBL" id="JAMZMK010006856">
        <property type="protein sequence ID" value="KAI7747038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CRV9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd17942">
        <property type="entry name" value="DEADc_DDX18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000379">
        <property type="entry name" value="RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044773">
        <property type="entry name" value="DDX18/Has1_DEADc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025313">
        <property type="entry name" value="SPB4-like_CTE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24031">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13959">
        <property type="entry name" value="CTE_SPB4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01178">
        <property type="entry name" value="DUF4217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 9" id="KW-0347">Helicase</keyword>
    <keyword evidence="2 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="14">
        <location>
            <begin position="121"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="12">
        <location>
            <begin position="152"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="13">
        <location>
            <begin position="354"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="568"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="8">
        <location>
            <begin position="121"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="56"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="66"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="77"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="581"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="595"/>
            <end position="606"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU365068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAI7747038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="606" mass="68308" checksum="54FCC84E870179DA" modified="2024-05-29" version="1">MAVLENNPIHSKLENNKKKRKRNKNNNKSTTADEQSDLAAQEVAHVNGGDEEKVKQKTKKKKKKTKVVEDNNNKTETDEEEEKGLNTEEEADEDQHEEEEEEEEEEKKSSGGNGNGIMSNDPFTALPLSEATMKAILDMGFKYMTQIQARSIPPLLEGRDVLGAARTGSGKTLAFLVPAVELLYHARFAPRNGTGVVVICPTRELAIQTHAVAKDLLKYHSQTLGLVIGGAARKGEAERIVKGVNLLVATPGRLLDHLQNTKGFIYKNLKCFVIDEADRILEANFEEEMKQIIKILPKTRQTALFSATQTKKVEDLARLSFQTTPVYIDVDTGRSRVTNEGLQQGYCVVPSAKRFILLYSFLKRNLSKKVMVFFSSCNSVKFHSELLKYIQVDCFDIHGKQKQQKRTSTFFDFCKAEKGILLCTDVAARGLDIPAVDWIVQYDPPDEPKEYIHRVGRTARGEGAKGNALLFLIPEELQFLRYLKAAKVPVKEYEFDEKKMANVQSHLEKLVGTNYYLNKSAKEAYRSYLLAYNSHSMKDIFNVHRLDTQAIAASFCFSDPPKVNLNIDSNASKFRQKSRSRNGFSESNPYGRNRGGGDDKTQVVRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CSF4</accession>
    <name>A0AAD5CSF4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Exocyst complex component SEC5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_025233</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Component of the exocyst complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SEC5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006853">
        <property type="protein sequence ID" value="KAI7747047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CSF4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029175">
        <property type="entry name" value="EXOC2/Sec5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039481">
        <property type="entry name" value="EXOC2/Sec5_N_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13043">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT SEC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13043:SF4">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT SEC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15469">
        <property type="entry name" value="Sec5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="2" id="KW-0653">Protein transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst complex component EXOC2/Sec5 N-terminal" evidence="3">
        <location>
            <begin position="1"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU365069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF15469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7747047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="411" mass="47372" checksum="37C14706318E3EEB" modified="2024-05-29" version="1" fragment="single">MLKRVLEEVENVMHDFKRMLYESMEDPHIDLTNLENIVRLLLELEPESDPVRRYFGIQNRRIQILLDDCTFDHESRMENLQNKLHEKALSDENWKKIQQDLHESVDGSLVNSQPIDSGAEELDALRARYIRRLTAVIMHLIPPFWKVALSVSSGKFAKNKGRYFGHSLEVVSGMIHEIISAYDSKVQNTFLELEETNVICPYMSDAIMDISKACQAFDAKEAAPTIAVESLRALLFESTKLYIMKLCSWMRASMEEESRNETWVPVTVLERNKSPYAISSLPLAFRSVMVAAMDRIDMMIKFIRSEAASEDSLMMLQEIQESVTLSFFNCLLGFAGQMERIGGELGQIKQHLPFENGYYPDVVDEFSFHHRPGSISDQHQQLLMVLSNIGFCKDDLFGEMYNKYKHVFLKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CTE7</accession>
    <name>A0AAD5CTE7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_004681</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006949">
        <property type="protein sequence ID" value="KAI7746539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CTE7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47951:SF7">
        <property type="entry name" value="FLAVONOID 3',5'-HYDROXYLASE-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47951">
        <property type="entry name" value="OS08G0547900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0349">Heme</keyword>
    <keyword evidence="2 3" id="KW-0408">Iron</keyword>
    <keyword evidence="2 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="1 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="2">
        <location>
            <position position="397"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7746539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="459" mass="52814" checksum="4E5D2EAC1ABCD95D" modified="2024-05-29" version="1">MISEVAYEIYHTIITFWSWWWEVQNEQDELARAILTISVMILLLVCYQWKQSYSRNCTTPLPPGPYGLPVVGYLPFIGSNLHERFTKLAKTYGPIFSLRLGSKLHVVVNSMDLVKVVAHDMDQTFANRSPPLTALIMSYGGLDIVFSNNNAHWRNMRKVDMNEIAFNTSLNVVTSMLWGRSKSPEGKDSGYLGDRFREIEFKIVELLGAPNISDYFPMLKWFDLQRRQRDMQGLVEYVDRIFESIIEKRIKINSSAMDEVVEEDGRKDILQILLELTRDQKDAPTQSNGIHIKAFLLDLIIAATDTTSTVVEWVMAEILNNQGVMRKVQDELTYVIVGSYNIPKGTIVFMNIWAIQRDPKNWTNPLEFKPERFLKGNWDYKGNSLKFLPFGIGRRICAGIPLAEKMSMYIVASLLHSFDWMLPKDEDFELSDKFGIVTKKKKPLIAIPSQRLSDSNLFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CU80</accession>
    <name>A0AAD5CU80_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Scarecrow-like protein 23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_034019</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the GRAS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006537">
        <property type="protein sequence ID" value="KAI7748381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CU80"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005202">
        <property type="entry name" value="TF_GRAS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31636">
        <property type="entry name" value="OSJNBA0084A10.13 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03514">
        <property type="entry name" value="GRAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50985">
        <property type="entry name" value="GRAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="VHIID" evidence="3">
        <location>
            <begin position="156"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Leucine repeat II (LRII)" evidence="3">
        <location>
            <begin position="231"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="SAW" evidence="3">
        <location>
            <begin position="365"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="LxCxE motif" evidence="3">
        <location>
            <begin position="80"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="8"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="41"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7748381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="444" mass="49336" checksum="69DB647C704DF46E" modified="2024-05-29" version="1">MLQSLHLNHPPITTTTTTSPSSPNPMNPNPSKRPITDSTDEPTKRRNIDDDHKDKELDYTFDDQQAAAESSGLRLLGLLLQCAEHVAVDNLDQANNLLPEISELSSPFGSSPERVAAYFAEALQARIITSYLGTYTPLNNIKTLTLSQNHKIFNALQSYNSITPFIKFSHFTANQAIFQALEDKETAHIIDLDIMQGLQWPGLFHILASRPRKIKSIRLTGIGSSIELLEATGLRLHEFANSLNLPFEFVPLEHKIGNVSSDFVDQLGIRANETVVVHWLHHCLYDVTGSDYGTLRLLKTLKPKLVTIVEQDLSHAGSFLNRFVEALHYYSALFDAVGDGVEPDNVERFTVERQLFGSEIKNIVAVGGPKRTGEVKVERWGEELGRVGFRPVSLAGNPAAQASLLLGMFPWKGYTLVEETGRLKLGWKDLSLLTASAWEPSDLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CUH2</accession>
    <name>A0AAD5CUH2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_020731</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006512">
        <property type="protein sequence ID" value="KAI7748516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CUH2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000336">
        <property type="entry name" value="Cysteine-rich receptor-like protein kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000421">
        <property type="entry name" value="Serine/threonine-protein kinase receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052059">
        <property type="entry name" value="CR_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47973">
        <property type="entry name" value="CYSTEINE-RICH RECEPTOR-LIKE PROTEIN KINASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042245194" description="Protein kinase domain-containing protein" evidence="7">
        <location>
            <begin position="20"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="53"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7748516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="360" mass="40467" checksum="919D668A7FA31B05" modified="2024-05-29" version="1">MTIGVQLKALMKFWLPCFCSISDDKPANDKGQESATNLRLFSYSELNVACNGFGSRNKVGEGGFGTVYKGRLSDGTIVAIKVLSIELESMRGEREFISEIAALCDVQHENLVNLHGCCVEGAKRYLVYDYMENNNLAYQFLGGEQNRNSFSWTRRKNVAIGVAKALAYLHEEKTPHIVHRDIKASNVLLDHDFNPKVADFGLARLFYDDTSHISTRVAGTLGYLSPEYAITGRLTRKSDVYSFGVLLLEIITGRSIVEFDMERGEHFLVDKAWDMYTCDRLVELVDPLLLKCDDERLICEAVWFMKVGLLCVQEATKLRPRMSAVINMLSVETSVEGIKISKPGFLADLMDVKIDKKRSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CVB9</accession>
    <name>A0AAD5CVB9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_009902</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006417">
        <property type="protein sequence ID" value="KAI7748996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CVB9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37383">
        <property type="entry name" value="OS01G0694200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37383:SF1">
        <property type="entry name" value="OS01G0694200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7748996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="463" mass="50671" checksum="1CB70AFF8F78080C" modified="2024-05-29" version="1">MVILTHARQLRLSPPPPPPLTTIIFDPTSLSLALMHSDSSFSLYPHFSPFSPPINIIIPPSTPTFVSPPSSAATFLHIRSNDVVRLIFLVSSPHLAGSSILLRFYMLRTDNQFVRVRVVCNQSDLMFDEGKVGVIVKGNHGVSVKLASAINVFAMYSVSDRKVRVFAVKVVGDVVKLMRTAVIDCDLPVFSIGVSNGFLMLGEEGGVRVFNLRLLVKGRVVKSEKTKGRKVSLVNGMVVGSNGSSVLYAKKVNGSLDEKIGKHYDNSGLWIPVTIDIGSYIIIAKLKPVKMRQNSQEGGVRFVSFKGKELDDCKASKVPLEMSIKAISIQFLGHNKFLILNSVGELYLLSLSNYVSGSESVHEMKKLTLSMKVQNLAVLPDDSTSPQIVWVSDGQYTIHAIIVTDTTDSTANENDTKDIEEKIQRPATQVIFTSEKIQEIIPLSANAILLLGQANNIFAYAIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CVD1</accession>
    <name>A0AAD5CVD1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Selenoprotein F</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_028524</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the selenoprotein M/F family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006614">
        <property type="protein sequence ID" value="KAI7748020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CVD1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.50">
        <property type="entry name" value="Sep15/SelM thioredoxin-like domain, active-site redox motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038219">
        <property type="entry name" value="Sep15/SelM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039992">
        <property type="entry name" value="Sep15_SelM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014912">
        <property type="entry name" value="Sep15_SelM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13077">
        <property type="entry name" value="SELENOPROTEIN F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13077:SF6">
        <property type="entry name" value="SELENOPROTEIN F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08806">
        <property type="entry name" value="Sep15_SelM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0712">Selenocysteine</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Selenoprotein F/M" evidence="7">
        <location>
            <begin position="25"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00040775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7748020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="104" mass="12041" checksum="50B6AC619C509F82" modified="2024-05-29" version="1" fragment="single">ELLSDCRKCCTEDSDDSTSKIIYSGAVLEVCMRKLVFYPEVVSFIEDEKEKFPSVKVQYAFNAPPKLIMLDDAGQHKETIRIDNWKREHILDFLREKLKPTSAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CVQ1</accession>
    <name>A0AAD5CVQ1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Angiotensin-converting enzyme 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_028253</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006396">
        <property type="protein sequence ID" value="KAI7749198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CVQ1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006476">
        <property type="entry name" value="CHP01589_pln"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01589">
        <property type="entry name" value="A_thal_3526"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31871:SF62">
        <property type="entry name" value="ANGIOTENSIN-CONVERTING ENZYME 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31871">
        <property type="entry name" value="OS02G0137100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09713">
        <property type="entry name" value="A_thal_3526"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7749198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="279" mass="31117" checksum="D307208D56C3E02D" modified="2024-05-29" version="1" fragment="single">MSGGEVRNISPDDIQMVQNLIEQCLPYYMTLKQVIDILYQQEKIEPSFTELVWQRLEEQNHDFFKGYHLRLMVKDQIMEFNKLLDRQAALMQQLGSTGVGLQPKSNGSHLQPMHHNSTCYAPENTSIPLKTENMTTFSRGFNSCGLSIPSGDPQPHSRRIDIPPNMLLAPNSNVGITNSVSVKTEPNYLGNSRFIYGTDDNVLEARPSIGDTPVSSFSCLESDPHRLNGTLLNDGSLFGMLGPMPQNFGLSDSTPDFTNSSGENKRLDIPENLGFEDFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CVZ8</accession>
    <name>A0AAD5CVZ8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">14-3-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_009900</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 14-3-3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006417">
        <property type="protein sequence ID" value="KAI7748994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CVZ8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.190.20">
        <property type="entry name" value="14-3-3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000308">
        <property type="entry name" value="14-3-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023409">
        <property type="entry name" value="14-3-3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036815">
        <property type="entry name" value="14-3-3_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023410">
        <property type="entry name" value="14-3-3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18860">
        <property type="entry name" value="14-3-3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00244">
        <property type="entry name" value="14-3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000868">
        <property type="entry name" value="14-3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00305">
        <property type="entry name" value="1433ZETA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00101">
        <property type="entry name" value="14_3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48445">
        <property type="entry name" value="14-3-3 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00796">
        <property type="entry name" value="1433_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="14-3-3" evidence="3">
        <location>
            <begin position="8"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with phosphoserine on interacting protein" evidence="2">
        <location>
            <position position="63"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with phosphoserine on interacting protein" evidence="2">
        <location>
            <position position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000868-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7748994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="220" mass="24908" checksum="581614CBE82A97FE" modified="2024-05-29" version="1">MASTEASREENVYMAKLAEQAERYEEMVEFMEKVAKTVDVEELTVEERNLLSVAYKNVIGARRASWRIISSIEQKEESRGNEDHVKLIKDYRATIETELSKICDGILGLLESHLVPSASSAESKIFYLKMKGDYYRYLAEFKTGADRKEAAESTLLAYKSAQDIALSDLAPTHPIRLGLALNFSVFYYEILNSPDRACNLAKQDETGDEIKESSKPEETQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CW69</accession>
    <name>A0AAD5CW69_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_027620</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the kiwellin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006377">
        <property type="protein sequence ID" value="KAI7749386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CW69"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd22270">
        <property type="entry name" value="DPBB_kiwellin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="entry name" value="RlpA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039271">
        <property type="entry name" value="Kiwellin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33191:SF77">
        <property type="entry name" value="RIPENING-RELATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33191">
        <property type="entry name" value="RIPENING-RELATED PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24300">
        <property type="entry name" value="KWL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="Barwin-like endoglucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7749386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="112" mass="12115" checksum="900B3FBD84FC6429" modified="2024-05-29" version="1">MTINNFEKGGDGGGPSECDGNYHSNDTPIVALSTGWYNKGKRCFKFINIHYKGKSVKAKVMDECDSTLGCDAEHGFQPPCPNNVVDGSKAVWEALGVPKSDWGEAEVTWSDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CXD0</accession>
    <name>A0AAD5CXD0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Acyl carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_033733</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Carrier of the growing fatty acid chain in fatty acid biosynthesis. May be involved in the synthesis of short and medium chain fatty acids. Accessory and non-catalytic subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), which functions in the transfer of electrons from NADH to the respiratory chain.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Complex I is composed of at least 49 different subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acyl carrier protein (ACP) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006308">
        <property type="protein sequence ID" value="KAI7749672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CXD0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000035">
        <property type="term" value="F:acyl binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000036">
        <property type="term" value="F:acyl carrier activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1200.10:FF:000003">
        <property type="entry name" value="Acyl carrier protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01217">
        <property type="entry name" value="Acyl_carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003231">
        <property type="entry name" value="ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00517">
        <property type="entry name" value="acyl_carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002148">
        <property type="entry name" value="PRK00982.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20863">
        <property type="entry name" value="ACYL CARRIER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20863:SF60">
        <property type="entry name" value="ACYL CARRIER PROTEIN 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="9 12" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="4 12" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="3 12" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Carrier" evidence="13">
        <location>
            <begin position="43"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00057783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00063067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7749672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="123" mass="13787" checksum="261E4708193DAF8E" modified="2024-05-29" version="1">MQSLRDSVLAYLRVGVFRRHVVLMQHVSGLNRLLCTSTGSGREQVIDRVIGLVKKFDKIDAAKVTESADFQKDLSLDSLDRVELVMAFEQEFSIEIPDEHADKLKCCADVANYIQSAPKSEDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CZY1</accession>
    <name>A0AAD5CZY1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_002883</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005932">
        <property type="protein sequence ID" value="KAI7751323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CZY1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018790">
        <property type="entry name" value="DUF2358"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36334">
        <property type="entry name" value="PROTEIN, PUTATIVE (DUF2358)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36334:SF1">
        <property type="entry name" value="PROTEIN, PUTATIVE (DUF2358)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10184">
        <property type="entry name" value="DUF2358"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7751323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="278" mass="31177" checksum="C7A4E3A9EE3F6D4F" modified="2024-05-29" version="1" fragment="single">MTAVTASVIPNLSVNPSVSSLRSITCHVTFRRRLHTVRVANDSKATEVSPDLIIEKSEGDRIVDGMEFSELCNEFECISSPAVEATARQLVRDILELREGNRALGTYAVSVKYKDPVRSFTGREKYKRLLWSTGALDKPNVDMVMLSTSVLNIKWTLKGTPKNPLASVGGPLILKVDSRFTLNQISGQVIEHEEIWDLSSSSIIAQAFFWASRRLFSTTQSAKDFSDSLKNFSSRFSTEKENVDYYPDPSGDPTKFFQTEDDFQRDAIQLALFLALLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D0E6</accession>
    <name>A0AAD5D0E6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Zeta toxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_012815</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005829">
        <property type="protein sequence ID" value="KAI7751733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D0E6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044802">
        <property type="entry name" value="NADKc-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010488">
        <property type="entry name" value="Zeta_toxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31153">
        <property type="entry name" value="CALMODULIN CALCIUM-DEPENDENT NAD KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31153:SF22">
        <property type="entry name" value="P-LOOP CONTAINING NUCLEOSIDE TRIPHOSPHATE HYDROLASES SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06414">
        <property type="entry name" value="Zeta_toxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zeta toxin" evidence="3">
        <location>
            <begin position="228"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF06414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7751733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="537" mass="61189" checksum="3AEF14C554110C74" modified="2024-05-29" version="1">MLMICEGIAFEIKEFLSSNILNLFDDDNIPTYTQIILASSIGLIIAAAMHIRFTKVIRSNKNIVPWIRVSKTRKPLKLEKFPDYVARQMGFHDTRECPYLCKLASDYIRKEEGCEEDMYSFFADDLQADSLFIKLVEEFERCILSYFAFHWTHASFMITQVLGPDGHEPKKKLKNIVMQATREQRFERVTKNLKVARLFTTLVEEMKAIGLASADDTECTDVMVPMAHKDRSPVLLFMGGGMGAGKSTVLKDLLKEPFWAGASANAVVIEADAFKESDVIYRALSSRGHHDMLHTAELARPVHQSSTDAASSLLVTALNEGRDVIMDGTLSWVPFVVQTITMARSVHRKRYRMGPGYKKNSDGSVTENYWEVEKKCDQIDGNQKRRPYRIELVGVVCDPYLAVIRGIRRAIMCRRAVRVRSQLTSHKRFANAFVTYCQLVDNARLYLTNALEGPPKLIGWKDKDKTLLVDPDEINILKTIERLNESANSIYELYKQTNPANEHGSVWNDIVMSPSRLIIQRELKNSIQKSEAIKHKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D178</accession>
    <name>A0AAD5D178_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Homeobox-leucine zipper protein GLABRA 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_009209</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HD-ZIP homeobox family. Class IV subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005766">
        <property type="protein sequence ID" value="KAI7752028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D178"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08875">
        <property type="entry name" value="START_ArGLABRA2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.530.20:FF:000026">
        <property type="entry name" value="Homeobox-leucine zipper protein GLABRA 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000229">
        <property type="entry name" value="Homeobox-leucine zipper protein HDG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042160">
        <property type="entry name" value="HD-Zip_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057993">
        <property type="entry name" value="HD-Zip_IV_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002913">
        <property type="entry name" value="START_lipid-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45654:SF24">
        <property type="entry name" value="HOMEOBOX-LEUCINE ZIPPER PROTEIN GLABRA 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45654">
        <property type="entry name" value="HOMEOBOX-LEUCINE ZIPPER PROTEIN MERISTEM L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25797">
        <property type="entry name" value="PDF2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01852">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00234">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50848">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 9 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 9 10" id="KW-0371">Homeobox</keyword>
    <keyword evidence="8 9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="12">
        <location>
            <begin position="85"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="START" evidence="13">
        <location>
            <begin position="248"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="9">
        <location>
            <begin position="87"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="45"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="47"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="68"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7752028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="741" mass="82930" checksum="432C0F1C57702D13" modified="2024-05-29" version="1">MSSSNDNNNNIINNNINPPTSTKHLFPSTSLSLTLGFSHKDDAAAAANTSVTEISSETSGPLRSRSDDDFDPDPDVDDGDDANKTKKRKKYHRHTAEQIREMEALFKESPHPDEKQRQQLSKRLGLHPRQVKFWFQNRRTQIKAIQERHENSLLKSELDKLGEENKLLRETIKKGICTNCGFGSSSKDVTSYVDEQQLRVENAKLRAEIEKLRTSVGKYPQGASPTNSCSVGNDHENKSSLDMCSGVFGLEKSRVMEIVNLAVEELVQMASAGEPLWIKSLETGREILNYDEYLSKFPSLDSSNVQRLRFIEASRDSGFVFVDLPRLVRSFMDVKEFEEMFPCMISKAATLDVICNGEGANRKGAVQLMFAELQMLTPLVVTREVYFVRYSKQLNIDKWAVVDISIDNVEKNIDASLARCRKRPSGCIIEEKSNGHCKVTWIEHWECQKNISHSMFRAIINSGLAFGARHWMATLQQQCERLVFFLATNVPTKDSSGIGTLAGRKSILKLAQRMTWSFSRAIGTSSYHTWKKIPSKTGYDIRVASRKNLNDPGEPLGVILCAVSSIWLPVSHTLLFDFLRDETRRNEWDIMSNGGPVQSIANLAKGQDRGNTVSIHTMKWKENMWMIQDSCTNTYESMVVCAPVAVNTMQSIMAGCDSSSIAILPSGFSILPDGIETRPLVISSKADDQSWEHGSLLTVGFQILTSDSPVSKLSMESVDSVNTLISSTLHNIKTGLQCEDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D330</accession>
    <name>A0AAD5D330_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Altered inheritance of mitochondria protein 32</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_028337</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005481">
        <property type="protein sequence ID" value="KAI7753293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D330"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03062">
        <property type="entry name" value="TRX_Fd_Sucrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000213">
        <property type="entry name" value="APD1p protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009737">
        <property type="entry name" value="Aim32/Apd1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31902">
        <property type="entry name" value="ACTIN PATCHES DISTAL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31902:SF21">
        <property type="entry name" value="SUCRASE_FERREDOXIN-LIKE FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06999">
        <property type="entry name" value="Suc_Fer-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="323"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7753293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="347" mass="38295" checksum="D0A0F6893F621CBD" modified="2024-05-29" version="1">MATTPENTTTDNISTESVIVDDDVTYGFQRPEMFTEKLTGSVNPYGRHVFLSYKSHEEWPARVESSESDPLPKRLAGAIKNRKNDLAVKTMLTVCESGDGTGLSDGDVLVFPDLVKYRGLEESNIDSFVEDVLVNSKPWSVGTEEAVTGSYVFVCAHNSRDKRCGVCGPVLIEKFNEEIGVRGLKDEVFVGACSHVGGHKYAGNLIIYSKVQDEKVSGHWYGYVTPSDVVDLLDQHIKKGEIIERIWRGEMGVPPVEKAEKAVEQTVPNGNGLKENTKDEKENTGGCCQGANGFSCCRDDNSEAKKSTYMFDFFTKKWEQHEIYTAAAVVGAVATVAVAYSLYKRSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D4B5</accession>
    <name>A0AAD5D4B5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_016966</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005657">
        <property type="protein sequence ID" value="KAI7752554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D4B5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7752554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="71" mass="8593" checksum="A114D2C210F3BDF7" modified="2024-05-29" version="1">MKAQVWGRGNEMNSHRPLDFRRLVQVRFLVNCLRDDIGHEYLQRKIFQRQGLRGVLIQSQEKKRFPDIITR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D4T5</accession>
    <name>A0AAD5D4T5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_023397</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005538">
        <property type="protein sequence ID" value="KAI7753120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D4T5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37204">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37204:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7753120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="328" mass="36662" checksum="32F14A7D1CF791C9" modified="2024-05-29" version="1">MARVRNWYNKSKAATVIWVISLITFYSIFRMASKYSSSSKVSVVSIKLYKRITLTRPNLAALPVSNGERSKLYEKMSRDLDEHGALFLKRGETSQSLLLSDLFDIKNGSVTPALKTANPPVRANVLHLSTEYSLPIAKAVRDIFSPTLNEVIWFQNSELYHFSMFHASHHISPVPASEEEIEAEVDAVKSVADQLCPVTILLDRVVLTSTGVLLGCWQVISGTDPVNIRSKLRNALPRAPEKQLYAPAILHTSLARILGHPKNLSEDTNAGSELQHFHELVARLNNRIVGTKVATISELWYVEEYDVLALALDGKMKTRRFQFGCSKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D7A7</accession>
    <name>A0AAD5D7A7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_018196</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010002997">
        <property type="protein sequence ID" value="KAI7754607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D7A7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034005">
        <property type="term" value="F:germacrene-A synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016102">
        <property type="term" value="P:diterpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046246">
        <property type="term" value="P:terpene biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00684">
        <property type="entry name" value="Terpene_cyclase_plant_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.600.10:FF:000007">
        <property type="entry name" value="Isoprene synthase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.130:FF:000001">
        <property type="entry name" value="Isoprene synthase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.130">
        <property type="entry name" value="Terpene synthase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034741">
        <property type="entry name" value="Terpene_cyclase-like_1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044814">
        <property type="entry name" value="Terpene_cyclase_plant_C1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001906">
        <property type="entry name" value="Terpene_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036965">
        <property type="entry name" value="Terpene_synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050148">
        <property type="entry name" value="Terpene_synthase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005630">
        <property type="entry name" value="Terpene_synthase_metal-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31225:SF187">
        <property type="entry name" value="LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31225">
        <property type="entry name" value="OS04G0344100 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01397">
        <property type="entry name" value="Terpene_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03936">
        <property type="entry name" value="Terpene_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01019">
        <property type="entry name" value="Terpene_Cyclase_Like_1_C_Termi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Terpene synthase N-terminal" evidence="3">
        <location>
            <begin position="60"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="Terpene synthase metal-binding" evidence="4">
        <location>
            <begin position="299"/>
            <end position="531"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7754607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="590" mass="68903" checksum="CBD23F5D842CCBDB" modified="2024-05-29" version="1">MTSTWTLSSPFLFCTSSIPRRNINFTYNKRLCSPRSKLANATTSMETSIRRSANYAPSLWSYDFVQSLSSKYTGEKCMTRSSTLKEVVRGMIRNEVGENPLALLNLVDDLQRLGLSYHFVDEISNVLEIIYINCYKSDKKWSKMDLNLKSLGFRLLRQHGYHIPQEIFKDVIDENGNVKEHFYKDVVGMLNLYEASYHSVEDESILDDARISTEKYLKEVIENITDETMLSLIRHALNFPLHWIPPRVETMWFLQMCERRSDMNPEVLELAKLDFNMVQAVHQEDLRYASSWWKETCWERFGFGRDRIVENFMWSVAENYLPHFKGRRDLTKVNAMITTIDDVYDVYGTLQELEQFTNVMNSWDVNAVKQLPHYMQICFHAFYNTINEMSYNVLTKEEAFILPYFQKAWQDLSNSYIIEARWFDSGYTPTFDEFLNNAYMSIGIVPIIKHAYLLTLTNVSEYALNRIGIAENMIRNACLIVRLTNDMGTSSDELERGDVPKSIQCYMHETGASEVEARHYIKTLILDTWKKLNKERRAIASEFSHEFLDCVVNLARMGHFMYTDGDKHGKPDMFKPYALSLFVNPIEGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D921</accession>
    <name>A0AAD5D921_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_008764</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the chalcone isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000578">
        <property type="protein sequence ID" value="KAI7756108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D921"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009570">
        <property type="term" value="C:chloroplast stroma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005504">
        <property type="term" value="F:fatty acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016872">
        <property type="term" value="F:intramolecular lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.890.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016088">
        <property type="entry name" value="Chalcone_isomerase_3-sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016089">
        <property type="entry name" value="Chalcone_isomerase_bundle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036298">
        <property type="entry name" value="Chalcone_isomerase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47698">
        <property type="entry name" value="FATTY-ACID-BINDING PROTEIN 3, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47698:SF2">
        <property type="entry name" value="FATTY-ACID-BINDING PROTEIN 3, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54626">
        <property type="entry name" value="Chalcone isomerase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7756108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="166" mass="17452" checksum="4EA2E34324D37E75" modified="2024-05-29" version="1">MIGEVAAVPRSLWLHVHPHIKTASLHSIHLSIPTNGTQFSEKIRIFSTKLSLQKLTIVTAAAAAASSVGNTDYAEEPATSVKFPTSLDLPGCSTSLSLIGTGFREKVFAIIGVKVHISTDGTPSAAEATIESANVTSSLFDVFFGRVPVSPTLKSSISNSLASFLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D9I1</accession>
    <name>A0AAD5D9I1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 6">Inositol-pentakisphosphate 2-kinase</fullName>
            <ecNumber evidence="1 6">2.7.1.158</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_027729</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Phosphorylates Ins(1,3,4,5,6)P5 at position 2 to form Ins(1,2,3,4,5,6)P6 (InsP6 or phytate).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>1D-myo-inositol 1,3,4,5,6-pentakisphosphate + ATP = 1D-myo-inositol hexakisphosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20313"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57733"/>
            <dbReference type="ChEBI" id="CHEBI:58130"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.158"/>
        </reaction>
    </comment>
    <comment type="domain">
        <text evidence="6">The EXKPK motif is conserved in inositol-pentakisphosphate 2-kinases of both family 1 and 2.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.158" evidence="1 6"/>
    <dbReference type="EMBL" id="JAMZMK010001136">
        <property type="protein sequence ID" value="KAI7755422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D9I1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035299">
        <property type="term" value="F:inositol-1,3,4,5,6-pentakisphosphate 2-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032958">
        <property type="term" value="P:inositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.110">
        <property type="entry name" value="Inositol-pentakisphosphate 2-kinase, N-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009286">
        <property type="entry name" value="Ins_P5_2-kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043001">
        <property type="entry name" value="IP5_2-K_N_lobe"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14456">
        <property type="entry name" value="INOSITOL POLYPHOSPHATE KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14456:SF2">
        <property type="entry name" value="INOSITOL-PENTAKISPHOSPHATE 2-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06090">
        <property type="entry name" value="Ins_P5_2-kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 6" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="513"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU364126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7755422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="533" mass="59492" checksum="0CF5CA226ABA30BF" modified="2024-05-29" version="1" fragment="single">EAKKHPPVLEIPEAVKTISAPGWLIYTGIPTPTSHGLGLELNAPLLIASNLKQQQEEMVELVLQPKDASDWVNYRGEGGLNIVLAYNGSSPNFIGKVLRVRKIRKKQSEGPSASAVALSEHERLVWKDVGHLLSAPTKDVANHAYAQFVLCPLLGSHHVDAGVRVLVTKEFLELIDNGVLLERPSWRVDNGGINTLLDSAILMSDHSMFPPHVCLLFSHPMTIKAVPEEEVCISVEIKPKCGFLPTSRFIREENAAKRRISRFRLHQILKFHQKKISQISEYDPLDMFSGSKGRVVKSVKDLFLTPQNNFRVFLNGRLVFGSLGGEVDDTNTQIAEAFEDTLKVFFKADDDGGMHIPEFLQLVAGAVSESGILDRLLQVQKLDAIDIEGAIHAYYDVMSQPCAVCKELQPLATDGFHSIPFDQSLKIVRDFLISLTAKDLSLMISFRPTPKGNSQSPLNGGTIYLESLNRSFDYKASFIDLDMKPLEKMPYYYKLDQEIVSCYTRMMKTVNHPEKSESLHQDESSAIEDKSHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DAS4</accession>
    <name>A0AAD5DAS4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_016497</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000097">
        <property type="protein sequence ID" value="KAI7757606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DAS4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051564">
        <property type="entry name" value="LRR_receptor-like_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48055">
        <property type="entry name" value="LEUCINE-RICH REPEAT RECEPTOR PROTEIN KINASE EMS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48055:SF27">
        <property type="entry name" value="LRR RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE FEI 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7757606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="116" mass="12919" checksum="F053F919DADC4C3B" modified="2024-05-29" version="1" fragment="single">YMQSGRATEKTDVYSFGVLMLEVVSRKRPTDASFIEKGLNIVGWLNYLVIEGRQHEIIDSDCEGVGQETLDPLLSVAIQCVSSNPEDRPTMHKVVKTLESDVMTPCPSDFYDSSSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DAY4</accession>
    <name>A0AAD5DAY4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_008866</name>
        <name evidence="1" type="ORF">M8C21_023267</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000152">
        <property type="protein sequence ID" value="KAI7757224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAMZMK010000070">
        <property type="protein sequence ID" value="KAI7757733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DAY4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47689">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT (TPR)-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47689:SF2">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT (TPR)-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7757224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7757733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="87" mass="9651" checksum="24934575787545D5" modified="2024-05-29" version="1">MLRRSEAEKALLDCISAYKQYGIQRSLYNLGDIKSEYLSCLKHLSALLTHSDSNGLKQSGRPSLQEVLNEIKRVEGGSSSSIPNKKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DBY3</accession>
    <name>A0AAD5DBY3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_009988</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000214">
        <property type="protein sequence ID" value="KAI7756944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DBY3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7756944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="104" mass="11218" checksum="C7339D35B50A22B8" modified="2024-05-29" version="1" fragment="single">GNSYNHSTGTDSGVPPLTATRLLFLAREQPKPHNQSKAHLRVVRGETVMLGFRVGGGGCSVREGHRAAHGDSPLKGGRKKQGFTAASHLSRRLYLNCKFEALLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DC92</accession>
    <name>A0AAD5DC92_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_021560</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000188">
        <property type="protein sequence ID" value="KAI7757052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DC92"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47989:SF43">
        <property type="entry name" value="CALCIUM_CALMODULIN-REGULATED RECEPTOR-LIKE KINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47989">
        <property type="entry name" value="OS01G0750732 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="92"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7757052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="328" mass="36558" checksum="ABE890D68923143F" modified="2024-05-29" version="1">MSQKTDLVVVGVCVGVAVGIFLASLVFFGVRWYKRRARLRRFANDRGTSTRPIRENGVNTSNALSESFSNSLTVNVSTYPAKTSPLYKWKKNRNEPVVSASDYQDTLTIYKATMPGGEVIAVKALAANSKQGDKEFQTEVSLLGRLHHRNLVNLVGYCVDKGQRMLIYEYMSNGSLASEEKDSLNWQQRLQIALDISHGIEYLHDGAVPPVIHRDLKSANVLLDHLMRAKAAMNSDGIDEILDKELVGDCDPEDVRSLARIAHRCLHKTPRKRPLIGEVSQAITKLKQRRLVREDSTIAFAGEEFSGVVGCIELQENQLKKMTSIAES</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DCI6</accession>
    <name>A0AAD5DCI6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_020626</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000518">
        <property type="protein sequence ID" value="KAI7756216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DCI6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010179">
        <property type="term" value="F:IAA-Ala conjugate hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009850">
        <property type="term" value="P:auxin metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017439">
        <property type="entry name" value="Amidohydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11014:SF55">
        <property type="entry name" value="IAA-AMINO ACID HYDROLASE ILR1-LIKE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11014">
        <property type="entry name" value="PEPTIDASE M20 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="163"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="175"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7756216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="424" mass="48109" checksum="16CCF9BE7F16E721" modified="2024-05-29" version="1" fragment="single">MKIRNDIRMVRQARRLVSKEVNHKFFVGRTKPKTRWKIYGIVAHDKENVLDTKLTQETLDYRLRERRQRDKEMKNNIRTSTPKKGIDNIFIYKVTYGSTTKFKTPKKCIKPYNVEKTSFSLVVSPVGSLTPLIQLALRLSWAKYKLFFLPHYRLHQHLFLQPQTTPASPPPSPLSFPQSPSAASSPSPSTPNTIPPLNCFTGFLMMRFLIGWWRSEGRYMRTLNWVRENLRHVGEELDKLGIGYKYPVAVTGVVGFIGSGQPSFVAIRADMDALPMQGFDFLCYGLEVISDNGADMDALPMQIRMLRQLGYCYKGLGLLLGCGIISNPLSSPAKSYFKCCSTTGATVTIFSIQQNGSIWVEVELETISRIGKGSRKFHCNSDLALNKMGQFGFKLNWKPFQGLKGRQKAQENSIATQEAVLEMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DID8</accession>
    <name>A0AAD5DID8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">shikimate kinase</fullName>
            <ecNumber evidence="5">2.7.1.71</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_010582</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>shikimate + ATP = 3-phosphoshikimate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13121"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.71"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 5/7.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Plastid</location>
            <location evidence="2">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the shikimate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.71" evidence="5"/>
    <dbReference type="EMBL" id="JAMZMK010000026">
        <property type="protein sequence ID" value="KAI7758135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DID8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004765">
        <property type="term" value="F:shikimate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00464">
        <property type="entry name" value="SK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001033">
        <property type="entry name" value="Shikimate kinase 2, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00109">
        <property type="entry name" value="Shikimate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031322">
        <property type="entry name" value="Shikimate/glucono_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000623">
        <property type="entry name" value="Shikimate_kinase/TSH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023000">
        <property type="entry name" value="Shikimate_kinase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21087">
        <property type="entry name" value="SHIKIMATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21087:SF16">
        <property type="entry name" value="SHIKIMATE KINASE 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01202">
        <property type="entry name" value="SKI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01100">
        <property type="entry name" value="SHIKIMTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01128">
        <property type="entry name" value="SHIKIMATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7758135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="266" mass="29276" checksum="F9B83EEB806D1269" modified="2024-05-29" version="1">MFPIPNNSMGLCTRFEKKRRVNMLKCNLERSIKPVIGHRAAGLKVSCTSQNPPASVLESGTVTTEEDLVLKKKSEEIEPCLRMMGSGKTTIGQILAEVLGYSFFDSDKLIEQSVGGTAVADIFKLRGEAFFRHNETEVLHKLSSMRRLVVSTGGGAVVRPINWKYMHKGISVFIDVPLDALAQRLTAVGTASRPLLHHESGDAYTQRLSKLWEDRSEAYGNAHVRVSLERIAAKLGHGDVCCINSLPNLAGAHPNRGLLEEMKPKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G3M8</accession>
    <name>A0AAD5G3M8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ENTH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_007025</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Clathrin-coated vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <location evidence="3">Clathrin-coated pit</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011343">
        <property type="protein sequence ID" value="KAI7727599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G3M8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030136">
        <property type="term" value="C:clathrin-coated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005545">
        <property type="term" value="F:1-phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032050">
        <property type="term" value="F:clathrin heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048268">
        <property type="term" value="P:clathrin coat assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072583">
        <property type="term" value="P:clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006900">
        <property type="term" value="P:vesicle budding from membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16987">
        <property type="entry name" value="ANTH_N_AP180_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.150:FF:000005">
        <property type="entry name" value="putative clathrin assembly protein At2g25430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.150">
        <property type="entry name" value="ANTH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011417">
        <property type="entry name" value="ANTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014712">
        <property type="entry name" value="ANTH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048050">
        <property type="entry name" value="ANTH_N_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045192">
        <property type="entry name" value="AP180-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22951">
        <property type="entry name" value="CLATHRIN ASSEMBLY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22951:SF12">
        <property type="entry name" value="OS05G0426100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07651">
        <property type="entry name" value="ANTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00273">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89009">
        <property type="entry name" value="GAT-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50942">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0168">Coated pit</keyword>
    <keyword evidence="8" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="4" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENTH" evidence="10">
        <location>
            <begin position="22"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="331"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7727599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="557" mass="63465" checksum="797BB84C9D787114" modified="2024-05-29" version="1">MESSNFRKAIGIFKDHTSRTLAKVATFSDLRVAIVKATDHKACLAKEHHIRQIINLTSYSRCHTASCVATISQRLCKTKDWVVAIKALILIQRLIQEGSPLFIQEVYITTNNGTHILDMSNFRDTAWANSWDFSAFVRAYALYLREQVDSRMEVYQGRYGVFSDTEGEDEQLSPGTIVVRSTLVWKMQNKQIYSRIQYLMKQLDFILGCQPTGAANNNRIVALALYPLVKQSFQLYYEITEIVFILLDRFEELDVIDSMSVFEIFCQVSKQFEELDIFYYWCIDACITRTSDYPEINKISQAKLDEMDQFIKQKIEIKQQELIHEPVLKLEPEPNHEPEPEPEPEQEPQTTTHLISIEPLPLIEGFKEKNKETKTEHVGDLLCFEDDAPTTQDHSDKLALALFGVGLATEASDKTAIQPWEAFNEPADWETALVQTSSKLSKEQPSLPGRFDTLVLYGMYQQGATQAAIDSRIMSIGSASSVAFGTTGMPTPQALLPPGEDPFAASFAIPPPSYVQISDMEKKQRLLMEEQMMWRQYQGQLRMTNMYMNQSVDNMVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G581</accession>
    <name>A0AAD5G581_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_018515</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011021">
        <property type="protein sequence ID" value="KAI7729295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G581"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.10:FF:000016">
        <property type="entry name" value="Heat shock protein 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="65"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="65"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="82"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7729295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="98" mass="10295" checksum="D479C7156EF832A1" modified="2024-05-29" version="1">MRNTIKDEKIGSKLPEADKKKVEDAVEEAIKWLDGNQLAEAEEFEDKMKELEGVCNPIIARMYQGGAGGPDMGGGMDDDGPSAGGASGGAGPKIEEVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G5N8</accession>
    <name>A0AAD5G5N8_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_014322</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010922">
        <property type="protein sequence ID" value="KAI7729790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G5N8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7729790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="78" mass="8281" checksum="C959E927505A0A0C" modified="2024-05-29" version="1" fragment="single">MAGKKPSSSASPITIGNCKVVIDANNFTTHSNQNTLRISLSRNSNILISVVEDALGKERNDVQRSSLGENGNILLTGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G6I6</accession>
    <name>A0AAD5G6I6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Costars domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_025349</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the costars family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010782">
        <property type="protein sequence ID" value="KAI7730404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G6I6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032970">
        <property type="term" value="P:regulation of actin filament-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1540:FF:000002">
        <property type="entry name" value="costars family protein ABRACL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1540">
        <property type="entry name" value="Costar domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044302">
        <property type="entry name" value="Costars"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027817">
        <property type="entry name" value="Costars_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038095">
        <property type="entry name" value="Costars_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46334">
        <property type="entry name" value="COSTARS FAMILY PROTEIN ABRACL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46334:SF1">
        <property type="entry name" value="COSTARS FAMILY PROTEIN ABRACL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14705">
        <property type="entry name" value="Costars"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01283">
        <property type="entry name" value="Costars"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Costars" evidence="2">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7730404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="91" mass="10192" checksum="EBEF4424142869CD" modified="2024-05-29" version="1">MNVEEEVEKLKVEIQRLGTKQEDGSYKVTFGVLFNDDRCANIFEALVGTLRAAKRRKLLTYDGELLLQGVHDNVLIILKPPTPHSPTTPAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G6K0</accession>
    <name>A0AAD5G6K0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_003029</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010818">
        <property type="protein sequence ID" value="KAI7730287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G6K0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046958">
        <property type="entry name" value="RBK1/2/STUNTED"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47987">
        <property type="entry name" value="OS08G0249100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47987:SF5">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7730287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="168" mass="18811" checksum="3227CC014A25E07A" modified="2024-05-29" version="1">MYGKVTDKIDVYAFGVVLLELLSGRKPISSEYPKGEESLVIWAKPILSSGKYSQLLDQTFGNDFDADQMERMALAANLCIRRAPRARPHMNSVLKVLHGDAEVTKWARLEVGSDARVQVSEEESNIRSHLNLALLDVEDSSLSMSSVEESVSLEDYLRGRWSRSSSFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G6M5</accession>
    <name>A0AAD5G6M5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_028831</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SCO1/2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010758">
        <property type="protein sequence ID" value="KAI7730509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G6M5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02968">
        <property type="entry name" value="SCO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000013">
        <property type="entry name" value="Blast:Protein SCO1 homolog, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003782">
        <property type="entry name" value="SCO1/SenC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12151:SF5">
        <property type="entry name" value="AT19154P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12151">
        <property type="entry name" value="ELECTRON TRANSPORT PROTIN SCO1/SENC FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02630">
        <property type="entry name" value="SCO1-SenC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0186">Copper</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="103"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="7">
        <location>
            <begin position="144"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="60"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="66"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="273"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="4">
        <location>
            <begin position="184"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR603782-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR603782-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7730509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="315" mass="35032" checksum="C2E7BB5B8142B4FC" modified="2024-05-29" version="1">MAAVLSKIAHLRRTYRLISSYTSSHLIPHHSNPIPIHRPLHQSDLVGSFPRLPRFLSTNANAHADSPVKEAASDSKSDQGKQNQSDDSHQSSEQGKPVRGGPISWLSFLLLVATGAGVVFYYDQEKKRHIEDINASSSAVKQGPSAGKAAIGGPFNLIDHNGRSVTENDFKGKWTLIYFGFTHCPDICPDELQKLAAAIDKIKAKAGYEIVPVFISVDPERDTVEQVGEYVKEFHPKLIGLTGDPDEIKKAARAYRVYYMKTEEEGSDYLVDHSIIMYLMDPNMDFVKFFGKNNDVDALSEGIINEIKQYKKVKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G6X1</accession>
    <name>A0AAD5G6X1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Pectate lyase</fullName>
            <ecNumber evidence="4 9">4.2.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_007645</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>Eliminative cleavage of (1-&gt;4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.</text>
            <dbReference type="EC" id="4.2.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="9">Binds 1 Ca(2+) ion. Required for its activity.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 2/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the polysaccharide lyase 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.2.2" evidence="4 9"/>
    <dbReference type="EMBL" id="JAMZMK010010493">
        <property type="protein sequence ID" value="KAI7731245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G6X1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030570">
        <property type="term" value="F:pectate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.10:FF:000009">
        <property type="entry name" value="Pectate lyase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018082">
        <property type="entry name" value="AmbAllergen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002022">
        <property type="entry name" value="Pec_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045032">
        <property type="entry name" value="PEL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31683">
        <property type="entry name" value="PECTATE LYASE 18-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31683:SF187">
        <property type="entry name" value="PECTATE LYASE 18-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00544">
        <property type="entry name" value="Pectate_lyase_4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00807">
        <property type="entry name" value="AMBALLERGEN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00656">
        <property type="entry name" value="Amb_all"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00710">
        <property type="entry name" value="PbH1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0106">Calcium</keyword>
    <keyword evidence="8 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Pectate lyase" evidence="10">
        <location>
            <begin position="213"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="domain" description="Pectate lyase" evidence="10">
        <location>
            <begin position="534"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7731245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="804" mass="88996" checksum="E135F66B180D1497" modified="2024-05-29" version="1" fragment="single">VLRKKYPQFGQLHNGSFMQQALRSPSARKYLIDHKCRDFTVRRHPRRAALDYFPARHGAAGASSSYSRASSLGARPSSIVSTLTSNAGVLRLVCKKHDIKSDQKIYLNQIPSKPINNYKDSSLNDSRRNLGFLSCGTGNPIDDCWRCDPNWEKNRQRLADCAIGFGKGAVGGRDGKIYVVTDSSDNDAVNPKPGTLRHAVIQDEPLWIIFQRDMVIKLKEELIMNSFKTIDGRGASVHIAGGPCITIQYVTNIIIHGINIHDCKPGGNAMVRSSPSHFGWRTISDGDGVSIFGGSHVWVDHCSLSNCADGLIDAIMGSTAITLSNNYMTHHDKVMLLGHSDTYEQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRFLAPDRRFSKESLSSSPPSSLAPLYNILSTSYNKCKGTSLNESRRNLGFFASCGTGNPIDDCWRCDPNWENNRQRLADCAIGFGKEAVGGRDGRIYVVTDSSDNDAVNPKPGTLRYAVIQDEPLWIIFQRDMVIKLKEELIMNSFKTIDGRGASVHIAGGPCITIQYVTNIIIHGINIHDCKPGGNAMVRSSPSHFGWRTISDGDGVSIFGGSHVWVDHCTLSNCADGLIDAIMGSTAITLSNNYMSHHDKAILLGHNDAYQHDKIMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWQMYAIGGSANPTILSQGNRFLAPDNRFSKEVTKHDNAPESEWRNWNWRSEGDLMLNGAYFVQSGVGANYPRTSSLVARPSSIVSRLTSNAGALSFDFGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G797</accession>
    <name>A0AAD5G797_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_028726</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010876">
        <property type="protein sequence ID" value="KAI7729958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G797"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7729958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="47" mass="5284" checksum="242BD8CAFE860281" modified="2024-05-29" version="1">MSSSSSQPISPIVHKERNRKKGVRRRRVGWPLWWSHGGLAAAAAFNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G983</accession>
    <name>A0AAD5G983_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Metal-nicotianamine transporter YSL6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_004002</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the YSL (TC 2.A.67.2) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009876">
        <property type="protein sequence ID" value="KAI7733930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G983"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035673">
        <property type="term" value="F:oligopeptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004813">
        <property type="entry name" value="OPT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045035">
        <property type="entry name" value="YSL-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00728">
        <property type="entry name" value="OPT_sfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31645">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER YGL114W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31645:SF0">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER YGL114W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03169">
        <property type="entry name" value="OPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="61"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="103"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="152"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="273"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="313"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="384"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="413"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="463"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="505"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="557"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="598"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="632"/>
            <end position="656"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7733930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="672" mass="73677" checksum="0A3995BAF0F36571" modified="2024-05-29" version="1">MGTPTRPSTVDITDPLLQEPTHIDSETIPEWKEQITLRGLVASALLGTLFCIITHKLNLTVGIIPSLNVAAGLLGFFFIKSWTTFLEKFGFSVRPFTRQENTVIQTCVVACYGLAFSGGFGSYLLSMDERTYNLIGADYPGNRAEDVKNPGLLWMIGFIFVVSFLGLFSLVPLRKVMVMDYKLTYPSGTATAMLINSFHTTSGAELARKQVSCLGKYLSISFVWSFFKWFFSGIGDSCGFDNFPSLGLTLFKNTFYFDFSPTYVGCGLICPHIVNCSVLFGAIISWGFLWPFITTRAGDWYPADLGSNDFKGLYGYKVFVAIALILGDGLYNLVKIIAISVKAVLNNSTRRQDLPVIKEIMDAEATKAQLEQQKRDEVFLKDRIPTWFAASGYVGLAAISTATMPLIFPPLKWYLVLCSYIIAPAFAFCNSYGTGLTDWSLASTYGKIGLFIISSLVGSNGGVLAGLAACGVMMSIVSTAADLMQDFKTGYLTLSSAKSMFVSQLVGTAMGCIIAPLTFWMFWTAFEIGTPDSPYKAPYAVIYREMAILAVEGFSELPKHCLALCCGFFVAALVLNLLRDWTPSKVARFIPIPMAMAVPFYIGAYFAIDMFVGTVILFVWERVNKKDSEDYAGAVASGLICGDGIWTIPSAILSIFRIDPPICMYFGPSVSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G9J2</accession>
    <name>A0AAD5G9J2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Altered inheritance of mitochondria protein 24, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_028917</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010296">
        <property type="protein sequence ID" value="KAI7732163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G9J2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.160.10">
        <property type="entry name" value="Mitochondrial biogenesis AIM24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002838">
        <property type="entry name" value="AIM24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036983">
        <property type="entry name" value="AIM24_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016031">
        <property type="entry name" value="Trp_RNA-bd_attenuator-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43657:SF3">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 24, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43657">
        <property type="entry name" value="TRYPTOPHAN RNA-BINDING ATTENUATOR PROTEIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01987">
        <property type="entry name" value="AIM24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51219">
        <property type="entry name" value="TRAP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7732163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="253" mass="27206" checksum="1628D49C33D9D7B7" modified="2024-05-29" version="1" fragment="single">MAAPFFSTPFQPFVYQSPEDAVTPFQILGSEAQIVQIMLKPEDKVSSRPGCMCYMSGSIQMENVYAPENEAGVWQWLFGKNVTKTYFLNSGSTDGFVGIAAPSLGRILPIDLAKFGGEILCQPDAFLCSIGDVKVSNTFDQRARNVVAGVEGFLRQKLSGQGLAFVVGGGSVVQKNLEVGETLSVDISSIIALSSTLNVQIKYNGPMRRAVFGGNNLVTAMVTGPGIVFIQSLPFPRLSQRIARTVTSPNNPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G9R0</accession>
    <name>A0AAD5G9R0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_012548</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009905">
        <property type="protein sequence ID" value="KAI7733797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G9R0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7733797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="83" mass="9343" checksum="8FAE9F2EBEAF5F7D" modified="2024-05-29" version="1" fragment="single">MHSKSHPYLLHSHSSLSAPFTTILICSIHLLGFQSKKLNGAGCRQIRRRISGRRQRAWDRVLLLIMYPDSVDDLLGTFGGRGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GAX6</accession>
    <name>A0AAD5GAX6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">XPG N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_031734</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the XPG/RAD2 endonuclease family. XPG subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009650">
        <property type="protein sequence ID" value="KAI7734754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GAX6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004520">
        <property type="term" value="F:DNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09868">
        <property type="entry name" value="PIN_XPG_RAD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006084">
        <property type="entry name" value="XPG/Rad2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001044">
        <property type="entry name" value="XPG/Rad2_eukaryotes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006085">
        <property type="entry name" value="XPG_DNA_repair_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16171">
        <property type="entry name" value="DNA REPAIR PROTEIN COMPLEMENTING XP-G CELLS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16171:SF7">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00752">
        <property type="entry name" value="XPG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00853">
        <property type="entry name" value="XPGRADSUPER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00066">
        <property type="entry name" value="XRODRMPGMNTG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00485">
        <property type="entry name" value="XPGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="XPG N-terminal" evidence="5">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7734754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="132" mass="15170" checksum="45D6C44DD6797DBA" modified="2024-05-29" version="1" fragment="single">MGVHGLWDILSPIGHRVSVETLAGNRVAIDASIWMVQFMKAMKDEHGEMVRNAHILGFFRRICKLLFLRTKLVFVFDGATPALKQRIVIARRRQRENAQAKIRKTAEKLLLNHLKAMRLKELGLIKMDLGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GD58</accession>
    <name>A0AAD5GD58_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="26" type="ORF">M8C21_017107</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="26">AA19_3_7</strain>
            <tissue evidence="26">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RLP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="26">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="EMBL" id="JAMZMK010009434">
        <property type="protein sequence ID" value="KAI7735823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GD58"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000309">
        <property type="entry name" value="Leucine-rich repeat receptor protein kinase MSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000213">
        <property type="entry name" value="Tyrosine-sulfated glycopeptide receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051824">
        <property type="entry name" value="LRR_Rcpt-Like_S/T_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48006">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN DDB_G0281931-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48006:SF102">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN DDB_G0281931-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 22" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="17 24" id="KW-0472">Membrane</keyword>
    <keyword evidence="13 22" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-0675">Receptor</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="10 24" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16 24" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="506"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="25">
        <location>
            <begin position="591"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="480"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="483"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="620"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="EMBL" id="KAI7735823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="842" mass="91908" checksum="218A2D33B10C5C84" modified="2024-05-29" version="1">MGWWSDTGSVVDCCSWVGVSCDESGDRVVGLSIPGRGLRGSIPPSLHNLTSLSFLNLSSNFLSGPLSSFPDSNSSNLISLDLSYNRLSGSLPNTFPPSLRFLNLSSNLFTGPLSLELHINRLNGTLPPSLTNCTSLHLLNLRVNSLEGTLSDFDFSRFRQLTILDLGENHFSGLLPKSIFSCKSLTAIRLATNKLKGEVPPEVLQLPSLSFLSLSNNSLTNISQAFTILQTHQKLTTLILSKNFFNETLPPAAVSGFHNLKIMGLGGCKLYGQIPTWLTSLINLEVIDLSQNNINGTIPGWLQTLPNLFYLDLSNNSLSGGFPVELTRLPALASQQVLGHVNSSYLELPVFVAPQNASYLQYNQLATLPPALYLASNNLSGDIPVEVGNMQSIHTLDLSQNNFTGTIPASISNLTNLEKLDLSHNLLSGEIPDSLSSLNFLSSFNVAYNNLTGSIPTGRQFDTFSNQNYEGNAWLCGPPMQKSCSSSSQSPTTDSSGHKKGPNKKMIVGLILGICFGVGITLTCLAIWILSKRRILPRGDPEVFHMDIASFNSTSAGDVPKDTSGVILFPNNAKDIKHLTITDILKATEDFSQANIIGCGGFGLVYKATLANGTKLAVKKLSGDMGLIAREFKAEVEALSTAQHKNLVSLQGYCVHEGSQLLIYSYMENGSLDYWLHEKADGAARLDWPTRLKIAQGASCGLGYMHQPYNTHVTTELVGTLGYIPPEYSQSWIATLRGDIYSFGVVMLELLTGKRPMEVFRPKESRELVVWVQQLKREGKHDEIFDPILRGKGFEEEMLQVLDVACMCVNVNPVKRPGIDEVVDWLHNINVGSNSSNHRIPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GD81</accession>
    <name>A0AAD5GD81_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_013976</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009085">
        <property type="protein sequence ID" value="KAI7737214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GD81"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="96"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="45"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="69"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7737214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="194" mass="19269" checksum="329F349CB5E20C8D" modified="2024-05-29" version="1">MLSESRVARKDLGLDLGGIGLGVGVGIGIGGSGSGSGAGAGSGSGSSSSSSSSSSSSSSGGGGGGAGSEAGSSAGSHAGSVPNWLREEIIKKKAVIGSSATEVSRQDTESNEDEVMDRKGDHVGGKSMDSNQSAEAEGDDEDYIEAAKTAAVNQEIKRILTEVLLKVAAELFDEIATNVLEEDDLNVEGCCQLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GDG7</accession>
    <name>A0AAD5GDG7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Lysine-specific demethylase JMJ25-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_007558</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the JARID1 histone demethylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008860">
        <property type="protein sequence ID" value="KAI7738175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GDG7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000118">
        <property type="term" value="C:histone deacetylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032454">
        <property type="term" value="F:histone H3K9 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045109">
        <property type="entry name" value="LSDs-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014977">
        <property type="entry name" value="WRC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12549">
        <property type="entry name" value="JMJC DOMAIN-CONTAINING HISTONE DEMETHYLATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12549:SF67">
        <property type="entry name" value="TRANSCRIPTION FACTOR JUMONJI_ASPARTYL BETA-HYDROXYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02373">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08879">
        <property type="entry name" value="WRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51667">
        <property type="entry name" value="WRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="WRC" evidence="10">
        <location>
            <begin position="8"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="8">
        <location>
            <begin position="261"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="JmjC" evidence="9">
        <location>
            <begin position="614"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="46"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="157"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="722"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="936"/>
            <end position="960"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="46"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="157"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="171"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="753"/>
            <end position="772"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7738175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="960" mass="109221" checksum="5C50C4EDD1FCD69A" modified="2024-05-29" version="1">MAENSGDLPDDQRCKRNDGRQWRCRRPVVEGRTMCEAHFLQGKLRQNKEPVPDSLKIERQRTRKSRKNQENTSGKSEIRGNENMGCSNLPRKRGGIEGEMSRSNSIRKRRKRSVKEENLKSCVDMSEDLDDALKKMNLKKGDLQLDLIRGYLNRQIEKKKKKKKKGKQPHNDNNNNNNNNNNKGDIIKELKYGRLEISQASTSTPPVVTVNNVKVGASGSGLLPTRFFRSKNIDRVPMATMQNLPNIKATAKAATKKCHWCRMCSYRVLVKCLNCKKHLFCEDCIRARSRDKAEVKKQCPVCNGTCSCRGCERGRSKEVKTKDLVVYSGEGKFDKTQQLLHVMHLLLPLIEQINLEKYDEMDIEAKIKGKDHSELQIQEALGSPKQQCCGFCKACIVDVHRSCGNCSEDGSIECPPKNLGGCGDGILGLVCHPSFDWTKDLEKSAKEIVSNHKFTNFFDFDTLYCGLCDGNDKIESENKGEVLTYNKSLYFSIKQDLGDKNLEHFTKHWVKGQPLIIRDAIKIDTELSWDPIFTFCMYLDRSVKSRNDQDVKLKNCSDWCEVETGRQQIFMGGKTHANVWNEKLKFKVWLSSGTFQEHFPRHYAAVMHALPLQEYTNPLTGVLNLAANVLPESQNVDLGPFVYISYGRPEDLVGSDFLTKLCYHAYDMVNILVHATERPISEKKLNKVKILMSKYSSQDRNESLRKNKNKYKIDEACRKSSFSSEVTQQSELEDNANGELTQTPNDADPCVSSDDDSSNCDSDDEDLCDDEYGNSSGNDGKVVDTCGAQWDVFRREDVEKLVEFLKKYSNELNSSYRSPKKVVHPILDESFYLDAFHKKYLKEEFDVEPWTFEQRIGEAVIIPAGCPYQVKKIKSCVNVVFESISPESASMCIKLSDEIRQLPVNHKAKGKMLDVKKMIIQSVNAAVEEIHKIPQAEINNPKSEQEDDIQCVVGDKQTKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GDR8</accession>
    <name>A0AAD5GDR8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RPW8 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_018314</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009275">
        <property type="protein sequence ID" value="KAI7736438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GDR8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008808">
        <property type="entry name" value="Powdery_mildew-R_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766">
        <property type="entry name" value="PLANT BROAD-SPECTRUM MILDEW RESISTANCE PROTEIN RPW8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766:SF32">
        <property type="entry name" value="POWDERY MILDEW RESISTANCE PROTEIN, RPW8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05659">
        <property type="entry name" value="RPW8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51153">
        <property type="entry name" value="RPW8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0611">Plant defense</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="RPW8" evidence="5">
        <location>
            <begin position="6"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7736438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="463" mass="52493" checksum="0039E6A6A61519FC" modified="2024-05-29" version="1">MSENLKMVTKNMYELPLQSVLLDLHKSVASQAKTTIKFRTLLKRLDKTLSSIELVMCECGRLARVLDRPEKETKMFIYYLENGKLTVLKCSKIKCWNVYKKFVHANKLIRLDRELVRFFQVELQDKLSVRFRGLDLVDEVGQVLSVAKRAGGFQGLPDVIVGLDFHVKEVKCMLLKEENKVVVVSAPGGCGKTTLAKMLCQDDEIKGIFGDNIFYVTVSRTSSVKTVVQKLFTHHLNVNNCEFRTDEEAKNELENMMRQMGSQKILLVLDDVWSESESLIQDLKFSIPGYSILVTSRFRFSSIGSTYELSMLNDEDARTLLCRSAFPCEGNRMDVPDDLVSKMVKCCKGFPLALMVIGASLRDQGVLKWKTTLQKLSEGQSIFELNSSLLLSLRASVDALEDSPIARDCFLDLGSFPEDEKISASALMDLWVELYNLDKEGMYTIEYLLELSSRNLLCLVLAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GEY8</accession>
    <name>A0AAD5GEY8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">S-acyltransferase</fullName>
            <ecNumber evidence="15">2.3.1.225</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Palmitoyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">M8C21_018836</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">AA19_3_7</strain>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13 15">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="15">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="15"/>
    <dbReference type="EMBL" id="JAMZMK010008689">
        <property type="protein sequence ID" value="KAI7738969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GEY8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000300">
        <property type="entry name" value="S-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000334">
        <property type="entry name" value="S-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24161">
        <property type="entry name" value="ANK_REP_REGION DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24161:SF17">
        <property type="entry name" value="PALMITOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9 14" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0564">Palmitate</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="350"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="393"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="14">
        <location>
            <begin position="63"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="14">
        <location>
            <begin position="96"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="14">
        <location>
            <begin position="163"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="14">
        <location>
            <begin position="196"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="17">
        <location>
            <begin position="312"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="14"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAI7738969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="545" mass="59696" checksum="9A37AEA379F1CE04" modified="2024-05-29" version="1">MSSEIEVVDEVQQSRDLNTPTNETTDEEILKDDVYTAAAYGDLEKLQRLVESEGCSVSQPDSLGYYALQWAALNNRAAAAQYIIQHGGDINATDLTGQTALHWSAVRGAVQVADLLLQEGAHIQAADVYGYQTTHVAAQYGQTALLYHIVTKWNADPDVPDNDGRSPLHWAAYKGFADCIRLLLFLDAYRGRQDKEGCTPLHWAAIRGNLEACTVLVQAGKKEDLMATDNTGLTPAQLASDKNHRQVAFFLGNARRVLDKRWDGNGILGRFSKLGLAPALIFKFAKDDSWLGVVCMDWCSIGKLGNGTILPIVRPIRSKHCSTCDRCVEQFDHHCPWVSNCIGKRNKRDFLGFLVVEVFAMLITGIVALTRILTDPSAPSSFGAWLNHAGNQHTGALLFLIADAFLFMGVGRNITTNEMANVNRYSYLRGPGGRFRNPYDHGCKKNCSDLFINGYNEDIELIEDAAQSSEIDMMPMSRNMNQLQNGSNGNDTHAIDVNNNISKAHNNHVHSSSCSHGHGNKTDSAPFGLGLGLGLGRNSRSVAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GH28</accession>
    <name>A0AAD5GH28_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_000702</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008597">
        <property type="protein sequence ID" value="KAI7739633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GH28"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="151"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="169"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7739633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="179" mass="20715" checksum="CCE1D8B8D6E8E812" modified="2024-05-29" version="1" fragment="single">EALSSRKEELPKESDANSADENDDDEASFDVRMRQQILKKKMELGDVPSKQKGHKESSKPRTREKSPPRSKAESPDDGPKVNKLLLKKKGIGSEVRAERMANADADLQLLNPHERERQLMKQKKRRRQGHEEDDNMSRRNDPNDYVVVDPLLEKGKEKFNKMQAKQKKREREWAGKSLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GIX7</accession>
    <name>A0AAD5GIX7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ubiquitin-like protease family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_010394</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Protease that catalyzes two essential functions in the SUMO pathway: processing of full-length SUMOs to their mature forms and deconjugation of SUMO from targeted proteins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C48 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007525">
        <property type="protein sequence ID" value="KAI7744490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GIX7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.130:FF:000006">
        <property type="entry name" value="Probable ubiquitin-like-specific protease 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.130">
        <property type="entry name" value="Ubiquitin-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003653">
        <property type="entry name" value="Peptidase_C48_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057375">
        <property type="entry name" value="ULP2A/B_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47764:SF2">
        <property type="entry name" value="UBIQUITIN-LIKE PROTEASE FAMILY PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47764">
        <property type="entry name" value="UBIQUITIN-LIKE-SPECIFIC PROTEASE 2B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02902">
        <property type="entry name" value="Peptidase_C48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25352">
        <property type="entry name" value="PH_ULP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50600">
        <property type="entry name" value="ULP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin-like protease family profile" evidence="7">
        <location>
            <begin position="269"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="59"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="680"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="67"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00057729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7744490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="711" mass="80521" checksum="6A663F9C779911D6" modified="2024-05-29" version="1">MQGMILLEARHTTPRPVSSTELRIDLSESEVNVAVMSASPSNNRLTSAIQDLLSSHDQLDEAGGMDTNENGSMCNRSSSSSDVADDDGAVAEPSSDHCIAGWEMDSEDTTTVVLYPDHVLYRGSCYTDCVISFTGGFIEMEGSEVDEDEKTFKCKWRIEDILHITSHWHERVEMAMVNIHVLAKHTKHAEIVECASGTELKFAVIGSSWHERFEAIVSLNTTYKALWTSMLDSEGHMVGNSGVYSLKYLPVFDRPFEEVVYPKGDADAVSISKRDFDLLQPDTFVNDTIIDFYIKYLKNKIEPDERRKLHFFNSFFFRKLADPDKDPIDASRGGVAYQRVKKWTRKVNLFEKDYVFIPVNYNYHWSVIVMCHLGEVATYEDEDVSESMKVPCVLHMDSLRGSHTGLQGFVQSYLKEEWKARQPVASEDIASRFDNLRFISLELPQQQNMFDCGLFLLHYVELFLEQAPLHFNPFKISKSFDFLNVDWFPPAEASLKRIVIQKLICDLLENRPQEPNPTADDDQRCHLSSLTTDAHKGNILNLSLMHRQSSQDHQQVEMPSSLIQFADDRNITVKPSFELEFNCATTFNECGSGSLLPIEKDMEQENGIMPDDSAIPCSSPDYHETVNDNHELLPEIPISGCDDSMEIVNGSSQLDSETDQPTFMDILMPGPSFKFPDMQIAGSENSSGLELDAQHPTKKMRMTQPRKISLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GKG4</accession>
    <name>A0AAD5GKG4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Metallo-beta-lactamase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_026157</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007017">
        <property type="protein sequence ID" value="KAI7746217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GKG4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd16272">
        <property type="entry name" value="RNaseZ_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46504">
        <property type="entry name" value="TRNASE Z TRZ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46504:SF2">
        <property type="entry name" value="TRNASE Z TRZ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12706">
        <property type="entry name" value="Lactamase_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="1">
        <location>
            <begin position="50"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7746217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="282" mass="31597" checksum="470C5C31633979C1" modified="2024-05-29" version="1">MEAKKKKGLVIEDYPIEGLSIAGHETCVILPTLSLAFDIGKCPQRAISQQFLFISHGHMDHIGGLPMYVATRGLYRMSPPTIIVPKVIKENVEKIFEAHRAMDQSELKHTLIGLDVGEEFYLRKDVKVKAFKTYHVIPSQGYVIYSVRQKLKPEYVDLPGNEIVKLKFSGVEITYTATSPEIAFTGDTTSDFIVDDNNTDVLKAKVLIMESTYVENTMTIENARDYGHTHLSEIVNYASRFENKAIVLIHFSARYQLNVIQEAVSALGPPLAGRVFALTEGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GKV2</accession>
    <name>A0AAD5GKV2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_033101</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007464">
        <property type="protein sequence ID" value="KAI7744699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GKV2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7744699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="136" mass="16169" checksum="9AAC5F2015559510" modified="2024-05-29" version="1">MIDQKQCSIQEFVNEYQHNQLRWISSPQQYLEAQLQMSEMCSLLNVTVLTSRVDKWRWDCSNDDEFWRVLCFWCDFISSKIQYRSLANTKDKKEDSCTVVKNVSLRRYWSLVELCLAELILGNAIETTPNSDLEER</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GNW4</accession>
    <name>A0AAD5GNW4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_027527</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006268">
        <property type="protein sequence ID" value="KAI7749862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GNW4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="127"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="127"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="154"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="164"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="217"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7749862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="229" mass="20899" checksum="13986C905EDC82A9" modified="2024-05-29" version="1" fragment="single">NVPKAAVAVVVAAETATSVVKAVICLENAPKVVGVVAVVAEAATNVVRVAICLENVLRAGEAAAVVGAVTSAVRVDICPGNVLRAGEVVAVEEVVTSVARVGICLVNAPKVGAVEGGSGGGGGGGYGGGGGGGGQSGGWSSGNQAGGWGSTKASDADAGKKDGGGGGGWGSSSGGGGDGWGSSSGGGGSGWGSSSGGGGSGWGSGGGDETGKKQGGDETGGWGSGKKSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GQI0</accession>
    <name>A0AAD5GQI0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">phenylalanine ammonia-lyase</fullName>
            <ecNumber evidence="4">4.3.1.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_023442</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This is a key enzyme of plant metabolism catalyzing the first reaction in the biosynthesis from L-phenylalanine of a wide variety of natural products based on the phenylpropane skeleton.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PAL/histidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.3.1.24" evidence="4"/>
    <dbReference type="EMBL" id="JAMZMK010005931">
        <property type="protein sequence ID" value="KAI7751345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GQI0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045548">
        <property type="term" value="F:phenylalanine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006559">
        <property type="term" value="P:L-phenylalanine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="entry name" value="Fumarase/aspartase (N-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001106">
        <property type="entry name" value="Aromatic_Lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10362">
        <property type="entry name" value="HISTIDINE AMMONIA-LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00221">
        <property type="entry name" value="Lyase_aromatic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="L-aspartase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0585">Phenylalanine catabolism</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7751345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="162" mass="17139" checksum="51B3D3DA185B36D4" modified="2024-05-29" version="1" fragment="single">MENGSHVHELCMKDPLNWGVAAEALTGSHLDEVKKMVAEFRKPVVKLGGETLTVSQVAGIAAANDGAAVKVELSETARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKNGGALQKELIRFLNAGIFGNGTESSHTLPHSATRAAMLVRINTLLQGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GRJ5</accession>
    <name>A0AAD5GRJ5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_027555</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005640">
        <property type="protein sequence ID" value="KAI7752667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GRJ5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7752667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="34" mass="3637" checksum="5C72FD6022C29866" modified="2024-05-29" version="1">MIKKLEKEIDDVDAKIGINGIGVGVAPVVMRDRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GRP1</accession>
    <name>A0AAD5GRP1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Acid phosphatase/vanadium-dependent haloperoxidase-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_024015</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006230">
        <property type="protein sequence ID" value="KAI7750041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GRP1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003832">
        <property type="entry name" value="DUF212"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31446">
        <property type="entry name" value="ACID PHOSPHATASE/VANADIUM-DEPENDENT HALOPEROXIDASE-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31446:SF29">
        <property type="entry name" value="ACID PHOSPHATASE_VANADIUM-DEPENDENT HALOPEROXIDASE-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02681">
        <property type="entry name" value="DUF212"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7750041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="138" mass="15261" checksum="4CC104BE394F8C9C" modified="2024-05-29" version="1">MPCMYKEKKWDTRRMLSSGGMPSSHSATVTSLAACIGLQEGVGSSSFAIAVVLACVVMYDATGVRLHAGRQAELLNQIVCELPPEHPLSNVRPLRDSLGHTPFQVWSQSSYLFRIINLYGSREKLDVLAIPFFESQFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GRQ5</accession>
    <name>A0AAD5GRQ5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Non-specific lipid-transfer protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_021165</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Plant non-specific lipid-transfer proteins transfer phospholipids as well as galactolipids across membranes. May play a role in wax or cutin deposition in the cell walls of expanding epidermal cells and certain secretory tissues.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the plant LTP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006218">
        <property type="protein sequence ID" value="KAI7750086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GRQ5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01960">
        <property type="entry name" value="nsLTP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.110.10">
        <property type="entry name" value="Plant lipid-transfer and hydrophobic proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036312">
        <property type="entry name" value="Bifun_inhib/LTP/seed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016140">
        <property type="entry name" value="Bifunc_inhib/LTP/seed_store"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000528">
        <property type="entry name" value="Plant_nsLTP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33076">
        <property type="entry name" value="NON-SPECIFIC LIPID-TRANSFER PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00234">
        <property type="entry name" value="Tryp_alpha_amyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00382">
        <property type="entry name" value="LIPIDTRNSFER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00499">
        <property type="entry name" value="AAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47699">
        <property type="entry name" value="Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="2 4" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042141663" description="Non-specific lipid-transfer protein" evidence="5">
        <location>
            <begin position="25"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical" evidence="6">
        <location>
            <begin position="28"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7750086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="116" mass="12582" checksum="85174D44C0C266F2" modified="2024-05-29" version="1">MVRMMKALFVMVACMVVTTPYTEAAINCRDLVTKLLSCLPYMQNGGNPSLSCCLGVKSINDAAGTAPDRRAACYCMKEASGSEPSINRNNSASLPDKCRISLPYMISPFTECSRVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GRZ8</accession>
    <name>A0AAD5GRZ8_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_032788</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006263">
        <property type="protein sequence ID" value="KAI7749908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GRZ8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7749908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="46" mass="5206" checksum="4182F51A770BC2B6" modified="2024-05-29" version="1">MTVDYSNTAGDRFVEMALLAFKLIDGGKNLDEFARDAHKKMKIAWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GT40</accession>
    <name>A0AAD5GT40_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Calmodulin-binding protein 60 B-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_015065</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the plant ACBP60 protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005706">
        <property type="protein sequence ID" value="KAI7752319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GT40"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080142">
        <property type="term" value="P:regulation of salicylic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046829">
        <property type="entry name" value="Calmod_bind_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046830">
        <property type="entry name" value="Calmod_bind_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046831">
        <property type="entry name" value="Calmodulin_bind_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012416">
        <property type="entry name" value="CBP60"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31713:SF100">
        <property type="entry name" value="CALMODULIN-BINDING PROTEIN 60 B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31713">
        <property type="entry name" value="OS02G0177800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20452">
        <property type="entry name" value="Calmod_bind_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20451">
        <property type="entry name" value="Calmod_bind_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07887">
        <property type="entry name" value="Calmodulin_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Calmodulin binding protein-like N-terminal" evidence="9">
        <location>
            <begin position="2"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="Calmodulin binding protein central" evidence="10">
        <location>
            <begin position="153"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="Calmodulin binding protein C-terminal" evidence="11">
        <location>
            <begin position="223"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="296"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="383"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="383"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="406"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF20451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF20452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7752319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="544" mass="61005" checksum="A19E4553067CA313" modified="2024-05-29" version="1">MSLPLYTGGKVEGEHGAAIHIVLVDANSGHVVTSGPESSIKLDVVVLEGDFNNEDDEGWTQEEFESHVVTEREGKRPLLTGDVQVTLKEGVGTLGELTFTDNSSWIRSRKFRLGLKVASGFCEGIRVREAKTEAFAVKDHRGELYKKHYPPTLNDEVWRLEKIGKDGSFHKRLNKEGIFKVKDFLRLVVRDSQKLRNILGSGMSNKMWEALLEHAKTCVLSGKFYVYYPEDNKNVGVIFNNIYELSGLIAGDQYQPADSLSDAQKVYVDTLVKKAYDNWNQVVEYDGKSLVSFKQPKRSGVSRNDYSMGSIEPPRSSNNHLLPPRHPVVIPSESLSVDSNLLLGGYNDSMDNVYQTGTQLVNTNPHVQGQYDINFASNGRHVSNSQQIQNNGYNDRSELALGPPQSSSSSSFHSSNASVQQQPNMNMFDDWSQNSRDKGLGDFMSEEEIRMRSHEMLENEDMQHLLRLFSMGGGHSGNVAEDGFSFPSFMPSPAPNFDFDDDRSRSGKAVVGWLKIKAAMRWGFFVRKKAAERRAQIVELDEDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GUK0</accession>
    <name>A0AAD5GUK0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_011592</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005586">
        <property type="protein sequence ID" value="KAI7752876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GUK0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47818:SF2">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47818">
        <property type="entry name" value="RNI-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7752876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="164" mass="17730" checksum="3A82A6CE9701813C" modified="2024-05-29" version="1">MRSFCNLCLKFVCLTSFPCSKFGASLGRFLRSGIQILDVKGVGLGLNGFLDAQNEISQDLSIVRINISGNAGSFRSAGFLSKVISHAPKLVSVDASSNWIPIESMPTICAFLKAGKGKLEHFNMRQNPLWNNPAIASLVAEYQVNGKPNILLSPPVATLYDNDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-04-02" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GUY1</accession>
    <name>A0AAD5GUY1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mediator complex subunit 15 KIX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_009892</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000341">
        <property type="protein sequence ID" value="KAI7756582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.246.20:FF:000003">
        <property type="entry name" value="Mediator of RNA polymerase II transcription subunit 15a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.20">
        <property type="entry name" value="Coactivator CBP, KIX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036529">
        <property type="entry name" value="KIX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036546">
        <property type="entry name" value="MED15_KIX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044661">
        <property type="entry name" value="MED15a/b/c-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33137">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 15A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33137:SF4">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 15A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16987">
        <property type="entry name" value="KIX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47040">
        <property type="entry name" value="Kix domain of CBP (creb binding protein)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mediator complex subunit 15 KIX" evidence="4">
        <location>
            <begin position="24"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="96"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="199"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="276"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="327"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="487"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="96"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="140"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="151"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="284"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="332"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="421"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7756582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="644" mass="71772" checksum="FE235B20584A7132" modified="2024-05-29" version="1">MESNNWRPTQGSSGAVVDPSMESVDWRAQLQADSRQRIVNKIMDTLKRHLPFSGNEGLQELKKIAVRFEEKIYTAATSQSDYLRKISLKMLTMENRSQNPMPDGMQSSSAANSVNPSDPGSQVIQQGQQLPISAPSNNIQSGQQMMSQGMHSNIGSAGMQGSSNLPSALPPGSGLQQSTMANVAGQNSNLQNMQNMSGVQQNNVGNTMGPGNFANQRQQMQGRQQLAXXXXKFTLSLSAEVASNGKADTPAAFNAITPTATESVTARSSSTIRYATHNDDDQSVLRQRPQQVQPTQQQVQSTQQNLMGQQANATNMQQNQLIGQQNNYSDMQQQQQQQQQQQRLMGQQNKLSSVQQQPQQQLIGQHNNLSSMQQQQSGQQQQLHGSQSGNSNMQTNQHSMQQMLQSSMPMQQQNHQSSAPLLPTQAQSSLTQLQQHQGMPHMQSQSGQLQQQVGMQQQPNLLQRDMQQRLPTSGVFQQQNVIDQQKQLFQQQRAMPEASSTSLDSTAQTGNSNGGDWQEEVYQKIKAMKDLYLLDLTDMHQKILGKLQQYDSLPQQPKNDQLDKLKMFKSMLERYIQLLQLPNKHNILANYKDKLGTYEKQIIQVINSHRRKPGGPQQQXXXXXXXXXXXXXXXXXSIDASSIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GX49</accession>
    <name>A0AAD5GX49_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosome-recycling factor, chloroplastic</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Ribosome-releasing factor, chloroplastic</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_031660</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Responsible for the release of ribosomes from messenger RNA at the termination of chloroplastic protein biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Plastid</location>
            <location evidence="2">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RRF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010001074">
        <property type="protein sequence ID" value="KAI7755486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GX49"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032544">
        <property type="term" value="P:plastid translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00520">
        <property type="entry name" value="RRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.40:FF:000001">
        <property type="entry name" value="Ribosome-recycling factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.132.20:FF:000017">
        <property type="entry name" value="Ribosome-recycling factor chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.20">
        <property type="entry name" value="Ribosome-recycling factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00040">
        <property type="entry name" value="RRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002661">
        <property type="entry name" value="Ribosome_recyc_fac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023584">
        <property type="entry name" value="Ribosome_recyc_fac_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036191">
        <property type="entry name" value="RRF_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00496">
        <property type="entry name" value="frr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20982:SF3">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOME RECYCLING FACTOR PSEUDO 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20982">
        <property type="entry name" value="RIBOSOME RECYCLING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01765">
        <property type="entry name" value="RRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55194">
        <property type="entry name" value="Ribosome recycling factor, RRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="9 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0934">Plastid</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Ribosome recycling factor" evidence="13">
        <location>
            <begin position="100"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="209"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7755486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="262" mass="29449" checksum="88D2FFAEF0ADC0B4" modified="2024-05-29" version="1">MYSLSPATSSVRSVNERPRISPKLRNRPTTLVSIQGNLLSWSSSVSCVKLTAVLVDKRVSQKRISTIRCATLEEIEAEKTLIEKNAKERMEKAIDSMLSSFNSIRTGRSNPAMLDKIEVEYYGSPVNLKTIAQISTPDSSSLLVQPYDKSSLKSIEKAIVNSDLGMTPNNDGEVIRLSLPQLTSERRKELSKVVAKQAEEGKVAIRNIRRDAIKAYDKLEKEKKLSEDNIKDLSADLQKVTDEYMKKIDTIFKQKEKELLTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GYD2</accession>
    <name>A0AAD5GYD2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Bidirectional sugar transporter SWEET</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_009653</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Mediates both low-affinity uptake and efflux of sugar across the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the SWEET sugar transporter family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000312">
        <property type="protein sequence ID" value="KAI7756671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GYD2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051119">
        <property type="term" value="F:sugar transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.290:FF:000001">
        <property type="entry name" value="Bidirectional sugar transporter SWEET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.290:FF:000003">
        <property type="entry name" value="Bidirectional sugar transporter SWEET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.290">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047664">
        <property type="entry name" value="SWEET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004316">
        <property type="entry name" value="SWEET_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10791:SF157">
        <property type="entry name" value="BIDIRECTIONAL SUGAR TRANSPORTER SWEET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10791">
        <property type="entry name" value="RAG1-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03083">
        <property type="entry name" value="MtN3_slv"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 10" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="6 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="30"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="54"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="115"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="149"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="177"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU910715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7756671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="284" mass="31199" checksum="CF3CA8DF845C0332" modified="2024-05-29" version="1" fragment="single">NIISFCVFLAPMPTFYGIYKKKSTEGFQSVPYSVALFSCMLLLFYGYLKTENGMMIITINSIGCAIETAYLVVFLIYATRKSLISTVKLLVLFNVLAFGVIVFTTRFATNPGPKRVAVVGWICAVFSVCVFAAPLSIMRLVIRTKSVDYMPFSLSLCLTLCAVVWFFYGLLIKDYYVATPNVLGFIFGVAQMILYVIYKDKNKQVRPMGQPKGEPVVATVVDLGAILEMQEKVGMEIGVVLEMQEKPMVAGGEADGGGEKKEEVAVKVICLDLDGDKTVIPSIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GZ02</accession>
    <name>A0AAD5GZ02_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Knottins-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_027664</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000017">
        <property type="protein sequence ID" value="KAI7758199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GZ02"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00107">
        <property type="entry name" value="Knot1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.10">
        <property type="entry name" value="Knottin, scorpion toxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008176">
        <property type="entry name" value="Defensin_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003614">
        <property type="entry name" value="Knottins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036574">
        <property type="entry name" value="Scorpion_toxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33147">
        <property type="entry name" value="DEFENSIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33147:SF129">
        <property type="entry name" value="DEFENSIN-LIKE PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00304">
        <property type="entry name" value="Gamma-thionin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00288">
        <property type="entry name" value="PUROTHIONIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00505">
        <property type="entry name" value="Knot1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57095">
        <property type="entry name" value="Scorpion toxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00940">
        <property type="entry name" value="GAMMA_THIONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042203759" description="Knottins-like domain-containing protein" evidence="5">
        <location>
            <begin position="21"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="Knottins-like" evidence="6">
        <location>
            <begin position="33"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7758199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="78" mass="8589" checksum="A49D83F000638075" modified="2024-05-29" version="1">MKSSMKLIATLLLVVMCLMANEMGGPIVVEARTCLSQSHKFKGACLSDTNCGNVCKSEGFPRGKCRGFRRRCFCVKHC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5H086</accession>
    <name>A0AAD5H086_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DELLA protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_025120</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Transcriptional regulator that acts as a repressor of the gibberellin (GA) signaling pathway. Probably acts by participating in large multiprotein complexes that repress transcription of GA-inducible genes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">The DELLA motif is required for its GA-induced degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the GRAS family. DELLA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000044">
        <property type="protein sequence ID" value="KAI7757903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5H086"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009740">
        <property type="term" value="P:gibberellic acid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1290">
        <property type="entry name" value="Transcriptional regulator DELLA, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038088">
        <property type="entry name" value="DELLA_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021914">
        <property type="entry name" value="TF_DELLA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005202">
        <property type="entry name" value="TF_GRAS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31636">
        <property type="entry name" value="OSJNBA0084A10.13 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12041">
        <property type="entry name" value="DELLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03514">
        <property type="entry name" value="GRAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01129">
        <property type="entry name" value="DELLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50985">
        <property type="entry name" value="GRAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0939">Gibberellin signaling pathway</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Transcriptional factor DELLA N-terminal" evidence="9">
        <location>
            <begin position="13"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="106"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="VHIID" evidence="6">
        <location>
            <begin position="231"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Leucine repeat II (LRII)" evidence="6">
        <location>
            <begin position="317"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="SAW" evidence="6">
        <location>
            <begin position="459"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="VHIID" evidence="6">
        <location>
            <begin position="262"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="112"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF12041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7757903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="540" mass="59777" checksum="C90EBF55E54C46C2" modified="2024-05-29" version="1">MWDDFTQPDTAVDELLAELGYQVKSSDMNDVAQKIEHLEGVFENDVALSQLVSDSVHYNPSDLSSYLESMISELNHNNNNINNSFVNDVVSSDDLQSIIYPPPSKKQKMLLSSSSSSSPSPTTVVSSSYNPNVNPTHNPTQIQNPNQIIIMDTQENGIRLVHTLMACAESVQKHDLKLAETLVNRACILAASQTGAMKKVALFFTQALACRIYNLNPNTPHDSPAFDDLLHMHFYETCPYIKFAHFTANQAILEAFAGQKKVHVIDFSIKQGLQWPALLQALALRPGGPPKFRLTGIGPPVHDNNNNINNNTDYLQEVGWKLAQLADNINVEFEYRGYVCESLADIEPGMLDLRDDEVVAVNSVFEMHKLLARPGSVEKVLGSVKEMNPVIVTVVEQEANHNGPVFMERFTESLHYYSTLFDSLESTVVNDGGVTVDNGGGDMVMSEMYLGKQICNVVACEGVERVERHMTLTQWKSWFDSAGLESVHLGSNAYKQASMLLALFGGGDGYRVEESNGCLMLGWHTRPLITTSAWKIGATR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BK39</accession>
    <name>A0AAD5BK39_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_021476</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012172">
        <property type="protein sequence ID" value="KAI7724665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BK39"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004525">
        <property type="term" value="F:ribonuclease III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030422">
        <property type="term" value="P:siRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00593">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1520.10">
        <property type="entry name" value="Ribonuclease III domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014720">
        <property type="entry name" value="dsRBD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000999">
        <property type="entry name" value="RNase_III_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036389">
        <property type="entry name" value="RNase_III_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14950">
        <property type="entry name" value="DICER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14950:SF49">
        <property type="entry name" value="RIBONUCLEASE 3-LIKE PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00035">
        <property type="entry name" value="dsrm"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14622">
        <property type="entry name" value="Ribonucleas_3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00358">
        <property type="entry name" value="DSRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00535">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69065">
        <property type="entry name" value="RNase III domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50137">
        <property type="entry name" value="DS_RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50142">
        <property type="entry name" value="RNASE_3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RNase III" evidence="5">
        <location>
            <begin position="5"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM" evidence="4">
        <location>
            <begin position="145"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM" evidence="4">
        <location>
            <begin position="230"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7724665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="307" mass="34244" checksum="FA6659572D37CCCD" modified="2024-05-29" version="1">MEDSIKAVEALLMYRFKNKKLLEEALTHSSCTDGVSYQRLEFLGDNVIALAISNFFYLNYPEVDAGVLSRLLAANVFEFLIEVDEEDMVVYGGQMKAPKVLADIVESVAAALYIDLGFNLQDMWMIFRHLLEPLVMLDVIMAEPQPITALYEACQKNGKQVDIQYRRNGDRTMASVFVDGAFVASRSSDTKENAKLHAAEAALAKITKFKSCDTGPQAIVDFNAAMEIDGAKHKLNELCNKKRWPKPTYKLEQESGPSHDRRYVSSVQLELPDATLFAKGNERSKVKDSENSAASVMFGDLQKSGYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BKH6</accession>
    <name>A0AAD5BKH6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_005493</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012129">
        <property type="protein sequence ID" value="KAI7724940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BKH6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055411">
        <property type="entry name" value="LRR_FXL15/At3g58940/PEG3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31639">
        <property type="entry name" value="F-BOX PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31639:SF315">
        <property type="entry name" value="LEUCINE-RICH REPEAT DOMAIN SUPERFAMILY, F-BOX-LIKE DOMAIN SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24758">
        <property type="entry name" value="LRR_At5g56370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="48"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7724940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="387" mass="45393" checksum="B16B7FC0433E7C2B" modified="2024-05-29" version="1" fragment="single">QSFAKHTFNYYILYLNEYNSRHCIVSVQQKEPLKMNTRSMYKSERMSLDRITTLPQPILETILCHLSTKEAVRTSILSREWRYNWTKIPKLELSPCMGVAVYEQTSDIANTRRCKPCYDIYQILLRHQGPIHELTLTLPEDNCLELDQIILHLSRNYNFKKLKLYGSDLLSWYKLPICLFSFHHIRDLVIYSLDLDLPPTFNGFSCLESLCLRGIGISIKSLLRLLSNSPSLKSFNLFVSEAYDTCTVNELLECLPMIEHLSLTMSPYISECVVLDSVPQELPTSLIHLRYLCFEDMCFDGGCHLPFLLAFIKRSPNLEKIKLENDWCEKRHEEYSVVWDEYSNVWLEHLNELKIVRFNNLKPEMDFVKFILARSPKLKNVIEETPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BKY5</accession>
    <name>A0AAD5BKY5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosomal protein L15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_003645</name>
        <name evidence="5" type="ORF">M8C21_018883</name>
    </gene>
    <organism evidence="5 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL15 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012115">
        <property type="protein sequence ID" value="KAI7724964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAMZMK010010197">
        <property type="protein sequence ID" value="KAI7732627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BKY5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1120.10:FF:000001">
        <property type="entry name" value="Ribosomal protein L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1120.10">
        <property type="entry name" value="Ribosomal protein l15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024794">
        <property type="entry name" value="Rbsml_eL15_core_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000439">
        <property type="entry name" value="Ribosomal_eL15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_uL23/eL15/eS24_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003269">
        <property type="entry name" value="PRK04243.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11847:SF4">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11847">
        <property type="entry name" value="RIBOSOMAL PROTEIN L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00827">
        <property type="entry name" value="Ribosomal_L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01384">
        <property type="entry name" value="Ribosomal_L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="Ribosomal proteins S24e, L23 and L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7724964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7732627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="204" mass="24208" checksum="7BC45A1E64246268" modified="2024-05-29" version="1">MGAYTYVSELWRKKQSDVMRFMQRVRCWEYRQLPSIVRVTHPTRPDKARRMGYKAKQGYVIYRVRVRRGGRKRPVPKGIVYGKPTNQGVTQLKFQRSKRSVAEERAGRKLGGLKVLNSYWLNEDSTYKYFEVILVDPAHAAIRNDPRINWICNPVHKHRELRGLTSAGKKYRGLRGKGHLNHKMRPSRRATWKRNNTLSLRRYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BMA2</accession>
    <name>A0AAD5BMA2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Core-2/I-branching beta-1,6-N-acetylglucosaminyltransferase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_025809</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012146">
        <property type="protein sequence ID" value="KAI7724883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BMA2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044174">
        <property type="entry name" value="BC10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003406">
        <property type="entry name" value="Glyco_trans_14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31042">
        <property type="entry name" value="CORE-2/I-BRANCHING BETA-1,6-N-ACETYLGLUCOSAMINYLTRANSFERASE FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31042:SF2">
        <property type="entry name" value="GLYCOSYLTRANSFERASE BC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02485">
        <property type="entry name" value="Branch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7724883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="415" mass="48407" checksum="165FB5E58962CC8C" modified="2024-05-29" version="1">MKRKLISSKLLLRNNNKWKTKIFALLLVGFCFSTFLLMEAQYSRIGVSSSSSSMFPTFVVQKPKIAFLFLARNRLPLDLVWDQFFQGENENRFSIYVHSRPGFLFNRLTTRSRYFLDRQVNNSIQIYWGEASMIQAEIILLQHALMDPFNERFAFLSDSCIPLYNFSYTYDYIMSASTSFVDSFADTKEGRYNPKMDPVIPVHDWRKGSQWVILTRKHAEIIVKDSVVFPMFQQHCQASRVYGVLFRKSLPEFWRDRPVPADNSKEHNCIPDEHYVATLLAHKGLEGELTRRSLTHTSWDISSSKGRERQGWHPVTYKLADATPMLIQSIKDIDNIYYETEYRREWCTSKGKPSPCFLFARKFTRPAAMRLLNMSALGVSEEASSNSLMRKEKEYPQLLSYFDVVGFIPPVLKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BMY7</accession>
    <name>A0AAD5BMY7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">HTH myb-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_025952</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MYB-CC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011698">
        <property type="protein sequence ID" value="KAI7726362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BMY7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000002">
        <property type="entry name" value="Myb family transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025756">
        <property type="entry name" value="Myb_CC_LHEQLE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006447">
        <property type="entry name" value="Myb_dom_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046955">
        <property type="entry name" value="PHR1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01557">
        <property type="entry name" value="myb_SHAQKYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31499:SF80">
        <property type="entry name" value="HTH MYB-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31499">
        <property type="entry name" value="MYB FAMILY TRANSCRIPTION FACTOR PHL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14379">
        <property type="entry name" value="Myb_CC_LHEQLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="8">
        <location>
            <begin position="169"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="155"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="225"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="318"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="162"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="229"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="318"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7726362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="378" mass="42078" checksum="B3E56D12490E7EDD" modified="2024-05-29" version="1">MSSIDGTVGHIVSSSSGMSTNLHISSEETCSKKVPFVNQSSEVLRSTAFRDFMKENNTSSWCTDSLPEFLDYPENIPIQSSNLQIMPSEDVGKPNDWQDWADQLITEDDGVTPNWNEILVDTAVPDQTPKMEFHVGKSSRTSLSLQVNSLVPASPGEACTPVTPSSCGSQSKPRMRWTPELHEAFVEAVNKLGGSERATPKGVLKLMKVEGLTIYHVKSHLQKYRTARYKPEPSAEGPSEKKPTPVQDLSSLDLKTSSEITEALRLQVEVQKRLHEQLEIQKNLQMRIEEQGKYLQMIFEKQCKFGIDAFQPTSSTLENTETELNNEISSPPAQTKPKPKPIKLGSPSSEKQEEEREPSESQPSKRAKLNELSSTESS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BMZ7</accession>
    <name>A0AAD5BMZ7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_021775</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011762">
        <property type="protein sequence ID" value="KAI7726074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BMZ7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7726074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="75" mass="8584" checksum="E5669F4B1FA212BB" modified="2024-05-29" version="1">MIFFNPLLPFHLPSPPRHQTNHEPTLLLESSAHVSPFDAVVITALHKEHGSNMYRWLQFQFEIIRALIGFTTSAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BNG3</accession>
    <name>A0AAD5BNG3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Disease resistance protein At4g27190-like leucine-rich repeats domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_014186</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011624">
        <property type="protein sequence ID" value="KAI7726632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BNG3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057135">
        <property type="entry name" value="At4g27190-like_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050905">
        <property type="entry name" value="Plant_NBS-LRR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463:SF198">
        <property type="entry name" value="RPP4C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23247">
        <property type="entry name" value="LRR_RPS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0611">Plant defense</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Disease resistance protein At4g27190-like leucine-rich repeats" evidence="2">
        <location>
            <begin position="2"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7726632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="153" mass="17533" checksum="11308EBD38FEB04C" modified="2024-05-29" version="1">MENLKQIWPCHLPTVEKNNVSMLRVISVRSCDSLTNIFPDNPLPMLNNLEVIKVYYCGSIESIFNIDFETVSEMDGYISRLRSITVDYLSNLRELWRMSGVNNSNILINGFQGVQSITISGCKRFKDIFTPVTTSFDLYALINYTADEVFRVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BP26</accession>
    <name>A0AAD5BP26_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">M8C21_007917</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="23">AA19_3_7</strain>
            <tissue evidence="23">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010011836">
        <property type="protein sequence ID" value="KAI7725901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BP26"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000217">
        <property type="entry name" value="probable LRR receptor-like serine/threonine-protein kinase At1g53430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.430:FF:000004">
        <property type="entry name" value="Putative leucine-rich repeat receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000044">
        <property type="entry name" value="Putative LRR receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000433">
        <property type="entry name" value="Putative LRR receptor-like serine/threonine-protein kinase isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.430">
        <property type="entry name" value="Galactose-binding lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051824">
        <property type="entry name" value="LRR_Rcpt-Like_S/T_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021720">
        <property type="entry name" value="Malectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48006">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN DDB_G0281931-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48006:SF81">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11721">
        <property type="entry name" value="Malectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="14 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-0675">Receptor</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="427"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="22">
        <location>
            <begin position="484"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="797"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="813"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="512"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="KAI7725901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="826" mass="91236" checksum="7E5850AA971528A2" modified="2024-05-29" version="1">MMSGIITEELGDLSSIERLFLNSNFFMGELPASFAKLTTMKEFRIGANNFSGKIPDFIGQWTSLQSLRIQASGMEGPIPSSITLLQNLTDLRISDLRGPDSLCPPFSNTTLFRNLILRSCNLIGRLPEYLAPQNLNVLDFSFNKLSGSIPTTYSELNLTDFIYLTGNLLSGRVPDWILNSGDDIDLSYNNFTYVDSTCIKRGTNLFASFSDDTISVGENSYEHDLQPGGPSYFDAAENRWGFSNTGHFLDDDNRDSYIVDNTSRLLMNNSELYMQARTSALSLTYYGFCLLSGSYNVSLHFAEISFTDDETYRSLGRRVFDIYIQGKLVEKNFDISERAGGIGKAIVIPYTVNVTNALDIRLYWAGNGTIDIPSRGTYGPLISAISVDPMSGGGTTSASGGAQEVVGRMCTDYFTVPKDGDGVSGGAVAGIVVGTLSLIILVLGVLWWQGYLRRGDSMELELNGLELNTGSYTLKQIKTATNNFDVANKIGEGGFGSVYKGVLPDGTLIAVKQLSSKSKQGNREFLNELGVISALQHPHLVKLYGCCIEGNQLLIAYEYMENNSLARALFGPKEWQLELDWPTRYRICIGIAKGLAFLHEESRLKIVHRDIKATNVLLDKNLNAKISDFGLAKLDEEDNTHISTRVAGTYGYMAPEYALRGYLTDKADVYSYGIVLLEIVSGRGNIADREKENNFVLLDQAISLKTSGNLMELVDPKLDNTYDIQEMTVVINLALACTAISPANRPAMSSVVSMLERRIVPQEFPVEQSRLMAEIDRDKILKQLEDMNESYDEEMSIPYTDSSTDPIDGLSDYVQNGPNDTNKLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BQB6</accession>
    <name>A0AAD5BQB6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TF-B3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_000240</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011626">
        <property type="protein sequence ID" value="KAI7726591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BQB6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd10017">
        <property type="entry name" value="B3_DNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.330.10">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003340">
        <property type="entry name" value="B3_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015300">
        <property type="entry name" value="DNA-bd_pseudobarrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044837">
        <property type="entry name" value="REM16-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31391:SF99">
        <property type="entry name" value="B3 DOMAIN-CONTAINING PROTEIN OS06G0194400"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31391">
        <property type="entry name" value="B3 DOMAIN-CONTAINING PROTEIN OS11G0197600-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02362">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01019">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101936">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50863">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TF-B3" evidence="6">
        <location>
            <begin position="163"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7726591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="314" mass="36069" checksum="090C3F39A4FA0CF3" modified="2024-05-29" version="1" fragment="single">QDTHLKIQTITPPPNHNTLLVHPATKSSFNFTTMVARKKPNYEDSRQKRLEENKKRMEELKLTVLAQSLRTISTPKPSPMKKVNRTPRKPVDLTVVRRSNRVADKPPANYKEVPVEPLGFRRSYGKSVRDLSNRVYASYEDREYATGRADELHSTLQSDFPSFVKPMLQSHVSGGFWLGLPRHFCKSYLPKHDEIMTLVDEDEAEWQTRYLPRKTGLSGGWKGFAEDHKLADGDALVFQLIKRTVFKLTIARRLEKLQQLVMRCIIQSTRIGISVIFSFLKCLACGEEQWRRGGTKLGGVAQGGEDTWQFPKEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BQG4</accession>
    <name>A0AAD5BQG4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_025439</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011328">
        <property type="protein sequence ID" value="KAI7727741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BQG4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7727741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="35" mass="3765" checksum="60798EF18D993752" modified="2024-05-29" version="1">MVNLKWLVIRVGDYVGYPLAGDLQSASIGSGMLIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BT16</accession>
    <name>A0AAD5BT16_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_002803</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011282">
        <property type="protein sequence ID" value="KAI7727958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BT16"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34775">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34775:SF4">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="546"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="217"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="439"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="579"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="277"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="579"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="592"/>
            <end position="602"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7727958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="664" mass="75183" checksum="12AD6081CD36D2FC" modified="2024-05-29" version="1">MASNRSPSPLSSRNCRNSEANSTTRKSFSGNPFPRPNVIITPKSSNPYTPSNTPTATDLTKRNSIARKSINNGSMFQDGKENRKDALRSPARSVSKNFMSPTISAASKFTPSPRKKILGEKNDVTRASFEFFGKESDLNSEPLVLEQMVRKNDELNESKIVEQTVEKMVEQQEIVVQNVSVDEVNPGLTNDDTEIVKNRSYYCCSPITSSPVIAADHDQQLPPYDPKKNFLSPRPQFLRYKPNPRIEFLLNKEEKDDEYEESDVTRLEDTFNLESDSSSETEEKEQESDKEAESGDDSVDSVNGSVGDLSETVLDEKPIDSKVEKVSKPRFFTLTCFSLSFTENPPVNLPIYEDVGFSEMYHESLRYAAFAKERFDDLVENVKVWSMDLVSYLYEQRSLLIPTQKIESLQFFNLTTSEIQEEFRFNRHIGTDYIQEFEEETERLDEEEEEEEEEVVSEEIDEESDLVEVVSEEPIFEQIHDDLDEVKEKSVNLDEGFVGKSEVVTEEQPINEQIQTNIDEAESEMVQSDVEIVSNVADSSSFSTQVIATVCVAAFSIVAMAVSSVFYMKKERLNESKTAKIRVNDDKMPEESGSSESISVQKGNKKKSSNKRESLASSTSEFSMSESLGSITTFERIAMKHKDEVMLTPIRRSSRLLKNQAICS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BU51</accession>
    <name>A0AAD5BU51_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Tubulin alpha chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_018506</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the tubulin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011014">
        <property type="protein sequence ID" value="KAI7729359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BU51"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.20:FF:000022">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002452">
        <property type="entry name" value="Alpha_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01162">
        <property type="entry name" value="ALPHATUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0493">Microtubule</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="9">
        <location>
            <begin position="5"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="10">
        <location>
            <begin position="143"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7729359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="237" mass="26111" checksum="6A44B61789A1DB2A" modified="2024-05-29" version="1">MARCQVTKLLGEPTVIDEVRTGSYRQLFHPEQLISGKEDAANNFARGHYTTGKEIVDLCLDRIRKLADNFTGLQGFLVFNAVGGGTGFGLGSLLLERLSVDYGKKSKLGRSLDIERPTYTNLNRLISQVISSLTASLRFDGALNVDVIEFQTNLVPYPRIHFMLSSYAPAISAEKACHEQFSVAEITNTAFEPSSMMAKCDPRHGKYMACCLMYRGDVVPKDVNAAVAMAFGQKLTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BX85</accession>
    <name>A0AAD5BX85_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_001269</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010553">
        <property type="protein sequence ID" value="KAI7731090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BX85"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7731090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="52" mass="6159" checksum="65DE36FEAB7CEEAA" modified="2024-05-29" version="1">MIWTINGSRRMQQQRITDAQVWANMQQLNKWVWTVDGCSNRYNRCRILCAGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BYD6</accession>
    <name>A0AAD5BYD6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TF-B3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_032432</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010442">
        <property type="protein sequence ID" value="KAI7731504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BYD6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10017">
        <property type="entry name" value="B3_DNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.330.10">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003340">
        <property type="entry name" value="B3_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015300">
        <property type="entry name" value="DNA-bd_pseudobarrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044800">
        <property type="entry name" value="LEC2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31140:SF70">
        <property type="entry name" value="B3 DOMAIN-CONTAINING PROTEIN OS11G0156000"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31140">
        <property type="entry name" value="B3 DOMAIN-CONTAINING TRANSCRIPTION FACTOR ABI3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02362">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01019">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101936">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50863">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TF-B3" evidence="7">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="156"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="161"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7731504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="199" mass="22932" checksum="09A4E24674D7B2C1" modified="2024-05-29" version="1" fragment="single">KLNRLVIPKQYAERYFPLSGVDQQKELLLCFEDEAGKEWKFRYSYWNSSQSYVLTKGWSRYVKEKKLDAGDVVLFERHRFDGWRRRHAGGAQDSYPPHHAYHLPNASLPCQYQHGSIHAGGVHGNVENNQRYVVASGNVPRRVRLFGVNLECPNECEPSDQDGSSQGGQSHIQYSSSSSNLTHNMNINLSQLRYHQHEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C1W7</accession>
    <name>A0AAD5C1W7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">E3 ubiquitin-protein ligase RNF123/RKP TPR repeat domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_008400</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009971">
        <property type="protein sequence ID" value="KAI7733475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C1W7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045129">
        <property type="entry name" value="RNF123/RKP/RSPRY1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057987">
        <property type="entry name" value="TPR_RNF123/RKP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13363:SF5">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13363">
        <property type="entry name" value="RING FINGER AND SRY DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25576">
        <property type="entry name" value="TPR_RNF123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="E3 ubiquitin-protein ligase RNF123/RKP TPR repeat" evidence="4">
        <location>
            <begin position="233"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7733475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="328" mass="37742" checksum="B31A6F4EA465B7D0" modified="2024-05-29" version="1" fragment="single">MSHQSHSISQLEETDRQLRERCSGEQLRRLKEARSVYREEVIDSVRHCAWYRVSLFARWKQRGMYAACMWTVQLLLVLSKNDLVFSYVPESYLETLVDCFHVLRKSDPPFVPAGMFIKQGLTSFVTFVVTHFSDPRILSAELRDLLLQSISVLVQYKEFLATFECNQAAIHSLSTSLLSSFDNRSWISVTNILIRLCKGCGFGLSKHGESSSSSCVFQNLLREACLKDEELFSAFLNRLFNTLSWAMTEFSVSIREMQEKGQMIEFQQRKCSVIFDLSSNLARVLEFCTCEIPQAFLLGADTNLRRLVELVVFVLNHLTSVTDPEFFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C2D0</accession>
    <name>A0AAD5C2D0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_028723</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010161">
        <property type="protein sequence ID" value="KAI7732786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C2D0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016760">
        <property type="term" value="F:cellulose synthase (UDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030244">
        <property type="term" value="P:cellulose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005150">
        <property type="entry name" value="Cellulose_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13301">
        <property type="entry name" value="X-BOX TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03552">
        <property type="entry name" value="Cellulose_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="190"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="217"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="731"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="764"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="800"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="843"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="884"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="914"/>
            <end position="932"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="576"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="576"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="311"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="654"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="281"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="282"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="311"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR605150-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR605150-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7732786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="953" mass="107409" checksum="84400D1419924F0E" modified="2024-05-29" version="1">MDTKGRLVAGSHNRNEFVLINADEVGSPRVDGDEDEEGFDDLDNEFDMAHGGSESVLSAHRPYNASGFANSEVDGATLKPEIPLLTYGQEGQKIYEGDAKMLKSFIIHTKTILWCIGQSQDLMEEWRKRQNEKLQMVKHQGDGGHNDGDVDDPDMPNLQDSVFVIVKGMDEGRQPLSRKLPISSSKINPYRMVILIRMAILGLFFHYRILHPVNDAYALWLISVICEIWFAVSWIFDQFPKWFPIERETYLDRLSLRYEKEGKPSELAPVDVFVSTVDPMKEPPLITANTVLSILAVDYPVDKVACYVSDDGAAMLTFEALSETSEFARKWVPFCKKFNIEPRAPEWYFAEKVDYLKDKVHPSFVRERRAMKREYEDFKVRINGLVTMAQKVPEDGWTMQDGTPWPGNDVRDHPGMIQVFLGNNGVHDIEGNELPRLVYIEIRVSAVISNAPYMLNVDCDHYINNSKALRESMCFLMDPTSGKKICYVQFPQRFDGIDRHDRYSNRNIVFFDINMKGLDGIQGPIYVGTGCVFRRQALYGYDAPPKKKAPGKTCNCLPKWVLCCCCLSRKKKAKGKSKEKSKEKSTKGKKSKDPSTQIHSLENIEEGIEDGGVPPGASSASLLKEAIHVISCGYEDKTEWGKEVGWIYGSVTEDILTGFKMHCHGWRSVYCIPKRPAFKGSAPINLSDRLHQVLRWALGSVEILFSRHCPIWYGYGCGLKPLERFSYINSVVYPLTSVPLLAYCTLPAVCLLTGKFIVPEISNYASILFMLMFLSIAVTSILEIQWGGVGIDDLWRNEQFWVIGGVSSHLFALFQGLLKVIAGVNTNFTVTSKGGDDGEFSELYIFKWTTLLIPPLTLLIMNIIGVIVGISDAISNGYESWGPLFGRLFFAIWVILHLYPFLKGMMGKQSGVPTILIVWSILLASIFSLLWVRVNPFLDRGGIVLEVCGLDCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C2H5</accession>
    <name>A0AAD5C2H5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Eukaryotic translation initiation factor 3 subunit J</fullName>
            <shortName evidence="4">eIF3j</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_018657</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the eIF-3 subunit J family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009929">
        <property type="protein sequence ID" value="KAI7733679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C2H5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005852">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.246.60:FF:000002">
        <property type="entry name" value="Eukaryotic translation initiation factor 3 subunit J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.60">
        <property type="entry name" value="Eukaryotic translation initiation factor 3 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03009">
        <property type="entry name" value="eIF3j"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023194">
        <property type="entry name" value="eIF3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013906">
        <property type="entry name" value="eIF3j"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21681">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21681:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08597">
        <property type="entry name" value="eIF3_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3 4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="42"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="64"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7733679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="225" mass="25123" checksum="D47F820D5C9361AA" modified="2024-05-29" version="1">MEDWEDEPAPIVLKKEPKSMWDDEDVDEDGIKDSWEDEDEPAPPPVVAPPPVVKAPKKSTVKPAQEKGKAVVEASKEVPLDPLEEKLRQQRLVEEADYKNTAELFASKKGDDKTIDTFIPKSENDFIEYAELISNKLRPFEKSFHYIGLLKHVMRLSMTSLKAADAKEVASSVSAIANEKLKAEKEAAAGKKKTGGKKKQLLVDKPDDEAVVNAGYDGYDDYDFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C2U3</accession>
    <name>A0AAD5C2U3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_031323</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009892">
        <property type="protein sequence ID" value="KAI7733874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C2U3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000554">
        <property type="entry name" value="protein indeterminate-domain 12-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000131">
        <property type="entry name" value="protein indeterminate-domain 5, chloroplastic-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055187">
        <property type="entry name" value="C2CH-3rd_BIRD-IDD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055185">
        <property type="entry name" value="C2CH-4th_BIRD-IDD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055186">
        <property type="entry name" value="C2H2-2nd_BIRD-IDD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031140">
        <property type="entry name" value="IDD1-16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10593:SF206">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10593">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE RIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22995">
        <property type="entry name" value="C2CH-3rd_BIRD-IDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22992">
        <property type="entry name" value="C2CH-4th_BIRD-IDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22996">
        <property type="entry name" value="C2H2-2nd_BIRD-IDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="97"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="20"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="267"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="333"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="372"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="55"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="267"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="286"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="381"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7733874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="413" mass="46835" checksum="26116222FDFD9CD5" modified="2024-05-29" version="1">MKGIFLDDNMSNLTSASNEAISLSSSSNRNDIGTMYPPPQMQQQQQSFNSVPMITTNNNNNNQTQTNKKKRNLPGNPDPEAEVVALSPKSLMATNRFMCEICNKGFQRDQNLQLHRRGHNLPWKLKQKSKLEVVRKKVYVCPEPSCVHHDPSRALGDLTGIKKHYSRKHGEKKWKCDKCSKRYAVQSDWKAHSKICGTREYRCDCGTLFSRRDSFITHRAFCDALAEETGRFSSSTLNNLPLHLPINFPLKTEPQLILQHPQLSFNVGSSTTTSHHNNHQLPSWLNPNHHQQHQPHQPQQQQQENPNLDHNNIHLPSPSSHMSATALLQKAAQMGVTMSSNPTPPSTASPPTIILNGSHQTLQHDHMCAPILSSHHHHHPNSGLSSLSSSDHNHHHFVQLNKERQQCKWKQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C6G8</accession>
    <name>A0AAD5C6G8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">Laccase</fullName>
            <ecNumber evidence="4 13">1.10.3.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Benzenediol:oxygen oxidoreductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Diphenol oxidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Urishiol oxidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">M8C21_008323</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">AA19_3_7</strain>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Lignin degradation and detoxification of lignin-derived products.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>4 hydroquinone + O2 = 4 benzosemiquinone + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:11276"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:17594"/>
            <dbReference type="ChEBI" id="CHEBI:17977"/>
            <dbReference type="EC" id="1.10.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
        <text evidence="13">Binds 4 Cu cations per monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Secreted</location>
            <location evidence="2 13">Extracellular space</location>
            <location evidence="2 13">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the multicopper oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.10.3.2" evidence="4 13"/>
    <dbReference type="EMBL" id="JAMZMK010009492">
        <property type="protein sequence ID" value="KAI7735383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C6G8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052716">
        <property type="term" value="F:hydroquinone:oxygen oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046274">
        <property type="term" value="P:lignin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13849">
        <property type="entry name" value="CuRO_1_LCC_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13875">
        <property type="entry name" value="CuRO_2_LCC_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13897">
        <property type="entry name" value="CuRO_3_LCC_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000053">
        <property type="entry name" value="Laccase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000062">
        <property type="entry name" value="Laccase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001117">
        <property type="entry name" value="Cu-oxidase_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033138">
        <property type="entry name" value="Cu_oxidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002355">
        <property type="entry name" value="Cu_oxidase_Cu_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034288">
        <property type="entry name" value="CuRO_1_LCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034285">
        <property type="entry name" value="CuRO_2_LCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034289">
        <property type="entry name" value="CuRO_3_LCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017761">
        <property type="entry name" value="Laccase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03389">
        <property type="entry name" value="laccase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF364">
        <property type="entry name" value="LACCASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00394">
        <property type="entry name" value="Cu-oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00079">
        <property type="entry name" value="MULTICOPPER_OXIDASE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00080">
        <property type="entry name" value="MULTICOPPER_OXIDASE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13" id="KW-0052">Apoplast</keyword>
    <keyword evidence="10 13" id="KW-0186">Copper</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 13" id="KW-0439">Lignin degradation</keyword>
    <keyword evidence="7 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 13" id="KW-0964">Secreted</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041779791" description="Laccase" evidence="13">
        <location>
            <begin position="27"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="16">
        <location>
            <begin position="37"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="14">
        <location>
            <begin position="170"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="15">
        <location>
            <begin position="407"/>
            <end position="539"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAI7735383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="556" mass="60732" checksum="5F7B6E8AB0D7E939" modified="2024-05-29" version="1">MAATLFRVVAVMVVLVTCAFPTTVECRIRNYKFNVIMTNTTKLCKSKQIVTVNGRFPGPTIVAREDDIVTIKVVNHVKYNVSIHWHGVRQLRTGWADGPAYITQCPIQPGGTYVYNFTLTGQRGTLWWHAHVLWLRATVHGAIVILPKLGVPYPFPKPNKEVVVVLGNSDTEAVINQAQKLGQAPNVSDAHTINGNPGPIASCLANGGFKLHVDQGKTYMLRIVNAALNEELFFRIANHKLKVVEVDAVYVKPFTTDTILIAPGQTTNAIVTATQKAGKYLVAVAPFMDAPIAVDNFTATASLHYSGTLTSTAIKLVAPPPQNSTPVANTFINSLRSLNSATYPAKVPLTIDHSLFFTIGLGINPCKTCVNGSRVVADINNITFVMPTTALLQAHYFNISGVFTDDFPSKPLIPYNYTGTQPTNFATNKGTKLYRLPYNSTVQLVLQDTGMIAPESHPIHLHGFNFFVVGRGIGNFNPNKDPKNFNLVDPVERNTVGVPAGGWSAIRFRADNPGVWFMHCHLEVHTTWGLKMAFIVDNGKGPNESVIPPPSDLPKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C7L7</accession>
    <name>A0AAD5C7L7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_015822</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009187">
        <property type="protein sequence ID" value="KAI7736816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C7L7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00265">
        <property type="entry name" value="MADS_MEF2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1810.10">
        <property type="entry name" value="Transcription factor, MADS-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050142">
        <property type="entry name" value="MADS-box/MEF2_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033896">
        <property type="entry name" value="MEF2-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002487">
        <property type="entry name" value="TF_Kbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002100">
        <property type="entry name" value="TF_MADSbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036879">
        <property type="entry name" value="TF_MADSbox_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48019">
        <property type="entry name" value="SERUM RESPONSE FACTOR HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01486">
        <property type="entry name" value="K-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00319">
        <property type="entry name" value="SRF-TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00404">
        <property type="entry name" value="MADSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00432">
        <property type="entry name" value="MADS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55455">
        <property type="entry name" value="SRF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51297">
        <property type="entry name" value="K_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50066">
        <property type="entry name" value="MADS_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MADS-box" evidence="7">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="K-box" evidence="8">
        <location>
            <begin position="86"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="86"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7736816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="205" mass="23681" checksum="0E0C11F1EFE798AB" modified="2024-05-29" version="1">MGRGKVELKRIENPTNRQVTFSKRRDGLFKKAIELSILCEAEVALLVFSSSGKAYQFSSDDMDRTIRRYRNEKGLHKMNSHGVRTIEVWKNEMDEMKKAIDTLETKHKHLAGEDLTTLGMKELKQLERQLKIGVDRVRSRKWRLLSEHHKSMQEENSILQKKIKLHELLSEGDENSGLDSSDHVIQRFIPDGQPLNMNQPGFPGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C8T8</accession>
    <name>A0AAD5C8T8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">pectinesterase</fullName>
            <ecNumber evidence="3">3.1.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_001256</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>[(1-&gt;4)-alpha-D-galacturonosyl methyl ester](n) + n H2O = [(1-&gt;4)-alpha-D-galacturonosyl](n) + n methanol + n H(+)</text>
            <dbReference type="Rhea" id="RHEA:22380"/>
            <dbReference type="Rhea" id="RHEA-COMP:14570"/>
            <dbReference type="Rhea" id="RHEA-COMP:14573"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17790"/>
            <dbReference type="ChEBI" id="CHEBI:140522"/>
            <dbReference type="ChEBI" id="CHEBI:140523"/>
            <dbReference type="EC" id="3.1.1.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the pectinesterase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.11" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010009079">
        <property type="protein sequence ID" value="KAI7737227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C8T8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030599">
        <property type="term" value="F:pectinesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042545">
        <property type="term" value="P:cell wall modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045490">
        <property type="term" value="P:pectin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000070">
        <property type="entry name" value="Pectinesterase_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31321">
        <property type="entry name" value="ACYL-COA THIOESTER HYDROLASE YBHC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31321:SF98">
        <property type="entry name" value="PECTINESTERASE 67-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01095">
        <property type="entry name" value="Pectinesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0063">Aspartyl esterase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pectinesterase catalytic" evidence="7">
        <location>
            <begin position="126"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7737227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="348" mass="38170" checksum="8DA2E1701D8A13BD" modified="2024-05-29" version="1" fragment="single">GDTVEVQPMDVRSPSENIQVLISLDSGNPEPLEVECGRSNGFNVGGEKLHGVVVNNNDGGHELHGKGRCVLFEVDINANSNSSGMYVDVKGSEKFTSIQDAIDSVPDGNQDWVLIHVNKGTYSSENNYDSSTFKVDADDFIAFGITFKNEAPTGIANTSHNQSVAAYVGGDKVAFYSCGFSSSHNTLLDNEGRHYYDQCYIQGAVDVIFGQARSVFHECEIFVVADKRIEIEGSVTAHTRTSVNENTGFVFLKGRIYGVGHVFLGRPKGEHSRVVFAKTYLSKTVSPQGWTDWNHHGSLENVYHAEYNCHGPGSSTEGRVRWLKKLSSEEAAPFLSTDFIDGKQWLKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CAT6</accession>
    <name>A0AAD5CAT6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_000968</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008930">
        <property type="protein sequence ID" value="KAI7737873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CAT6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000480">
        <property type="entry name" value="Pollen receptor-like kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046959">
        <property type="entry name" value="PRK1-6/SRF4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48007">
        <property type="entry name" value="LEUCINE-RICH REPEAT RECEPTOR-LIKE PROTEIN KINASE PXC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48007:SF67">
        <property type="entry name" value="POLLEN RECEPTOR-LIKE KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="232"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="317"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="264"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="597"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="266"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="623"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="346"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7737873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="632" mass="70852" checksum="34050EFED01AF53D" modified="2024-05-29" version="1">MLHMLQTCKHVLSDDSDITKLITFKSSLNNTEALANWDPNVAPCNMDKENWTGIICGKDGSVFGLRLENMGLTGTIDMDILSKLSGLRTISFANNSFAGLIPDVSKISLLRSVYLSNNNFSGAIRDDMFSGMSGMRRVEVQNNKFSGRVPASLTGLSILVDLQMQDNEFEGKIPNFEQKDLKVNFANNRLSGRVPSGLRNQDPNSFAGNNVCGPPVTTKCKSSNNKHTRKTLIIAIIGVLAILFIILLIFRKRKTPKNAYKNHQTMKLDRNNPYKTSSKELKKENDEENDAKRVEKSGNIEFVRSDIEVFELQDLLRASAEVLGGGSFGSTYKAVLLGGQAAVVVKRFREMNNVRKEEFYVHMTRLGRFSHPNLLPLVAFHYKKDEKLLITDFAENGSLASHLHVNRKPNEPGLDWPTRLKIIKGVTQGLAYLYNELPHLSLPHGHLKSSNVLLDNAFNPLLADYALSPVVSKRHAQECMVAYKSPEYTYHNRTTNKTDVWCLGILILEILTGKFPANYLKQGKGGSSDLETWVNSVVREEWTGEVFDKDMKGTKNSEGEMLKLLKIGMCCCEWDVDRRWDMTVAVRRIEELMERGGDDEYSSCTSDGDAYSSKGMTDDDFSFSKSVVDPKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CAW3</accession>
    <name>A0AAD5CAW3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ribose-phosphate diphosphokinase</fullName>
            <ecNumber evidence="2">2.7.6.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_011464</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>D-ribose 5-phosphate + ATP = 5-phospho-alpha-D-ribose 1-diphosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15609"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.6.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ribose-phosphate pyrophosphokinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.6.1" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010008779">
        <property type="protein sequence ID" value="KAI7738603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CAW3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002189">
        <property type="term" value="C:ribose phosphate diphosphokinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004749">
        <property type="term" value="F:ribose phosphate diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006015">
        <property type="term" value="P:5-phosphoribose 1-diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009156">
        <property type="term" value="P:ribonucleoside monophosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000007">
        <property type="entry name" value="Ribose-phosphate pyrophosphokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00583_B">
        <property type="entry name" value="RibP_PPkinase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000842">
        <property type="entry name" value="PRib_PP_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029099">
        <property type="entry name" value="Pribosyltran_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005946">
        <property type="entry name" value="Rib-P_diPkinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037515">
        <property type="entry name" value="Rib-P_diPkinase_bac"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002320">
        <property type="entry name" value="PRK01259.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002758">
        <property type="entry name" value="PRK02812.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01251">
        <property type="entry name" value="ribP_PPkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210">
        <property type="entry name" value="RIBOSE-PHOSPHATE DIPHOSPHOKINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210:SF41">
        <property type="entry name" value="RIBOSE-PHOSPHATE PYROPHOSPHOKINASE 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14572">
        <property type="entry name" value="Pribosyl_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13793">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01400">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00114">
        <property type="entry name" value="PRPP_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ribose-phosphate pyrophosphokinase N-terminal" evidence="12">
        <location>
            <begin position="84"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF13793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7738603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="388" mass="42034" checksum="30560E2D331B3796" modified="2024-05-29" version="1" fragment="single">MASLVLPSPSSCSTSSSSSLLCPRSFTRRLIHDCSRVPASVNNIRCEISKSLSGKPLVPILNDGMLPNFMQSGRVNNAVINGRLKIFSGTANSELSHEIARYMGLDLGMIKIKRFADGEIYVQLQESVRGCDVYLVQPTSPPANENLMELLVMIDACRRASAKNITAVIPYFGYARADRKTQGRESIAAKLVANLITEAGADRVLACDLHSGQSMGYFDIPVDHVYCQPVILDYLASKSICSNDLVVVSPDVGGVARARAFAKKLSDAPLAIVDKRRQGHNVAEVMNLIGDVKGKVAVMLDDMIDTAGTISKGAALLHEEGAREVYACCTHGVFSPPAIERLSSGLFQEVIVTNTIPLKEQHYFPQLTVLSVANLLGETIWRVHDDSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CCA9</accession>
    <name>A0AAD5CCA9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">alpha-1,2-Mannosidase</fullName>
            <ecNumber evidence="8">3.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_004921</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the glycosyl hydrolase 47 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.-" evidence="8"/>
    <dbReference type="EMBL" id="JAMZMK010008917">
        <property type="protein sequence ID" value="KAI7737959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CCA9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004571">
        <property type="term" value="F:mannosyl-oligosaccharide 1,2-alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000102">
        <property type="entry name" value="Mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001382">
        <property type="entry name" value="Glyco_hydro_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050749">
        <property type="entry name" value="Glycosyl_Hydrolase_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036026">
        <property type="entry name" value="Seven-hairpin_glycosidases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11742:SF6">
        <property type="entry name" value="MANNOSYL-OLIGOSACCHARIDE ALPHA-1,2-MANNOSIDASE IA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11742">
        <property type="entry name" value="MANNOSYL-OLIGOSACCHARIDE ALPHA-1,2-MANNOSIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01532">
        <property type="entry name" value="Glyco_hydro_47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00747">
        <property type="entry name" value="GLYHDRLASE47"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48225">
        <property type="entry name" value="Seven-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="48"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="516"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="364"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7737959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="553" mass="62828" checksum="830F7BDAEC613F58" modified="2024-05-29" version="1">MARTRSSSSIRWRYVNPAYYLKRPKRLALLFVAFVSISFFVWDRQTLVREHEEEILTLKEEVERLQIELGELKHEDVVSVKKTNTTTKKIDMKKMDGLATDPIEAERREKVKDAMLHAWTSYEKYAWGHDELQPQSKNGVDSFGGLGATLIDSLDTLYIMGLDEQFQRAREVVGGLLSAYDLSGDSVFLEKAKDIADRLLPAWDTPSGIPYNIINLAHGNAHNPGWTGGDSILADSGTEQLEFIALSQRTGDPKYQLKVENVILVLNKTFPDDGLLPIYINPHRGTHSHSTITFGAMGDSFYEYLLKVWIQGNKTAEVKHYRDMWETSMKGLLSLVRKTAPSSFTYISEKIGNSLIDKMDELACFAPGMIALGSSGYGPDESTKFLNLAEELAWTCYNFYQSTPTKLAGENYFFHSGQDMTVGTSWNILRPETVESLFYLWRLTGNKTYQEWGWNIFQAFEKNSRVESGYVGLKDVNTGVKDNMMQSFFLAETLKYLYLLFSPPSVISLDEWVFNTEAHPLKIVPRIVPGEERKPSNINIRARTRKEGRFGGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CCE6</accession>
    <name>A0AAD5CCE6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Chloramphenicol acetyltransferase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_003864</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008891">
        <property type="protein sequence ID" value="KAI7738019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CCE6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051504">
        <property type="entry name" value="Plant_metabolite_acyltrans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31625">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02458">
        <property type="entry name" value="Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7738019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="451" mass="50906" checksum="ED58BAFB25B36F3B" modified="2024-05-29" version="1">MGSFPILTVTEHAQVSPPPATIGDKLLPLTFFDFWWLTQPPVHYLFFYEMSVTQTQFAETIIPNLKQSLSITLQHFFPFVGNLVVYPTCTKNPEIRYVEGDSVAVTFAECDLDFNKLTGNNPRDCEMFYHLIPLLERVEKTPDYTKIPVFSIQVTLFPNFGISIGMTNHHCLGDASTRFNFLKAWTSIARCGTDESFLANETLPFYDRLVKNLKLDESYLKFAKVEKFKEEYQPSKLCGPTDKVRGTFILPRTTLNRLKTLVSTQLPTLTYVSSFTVACAYIWSCIAKTRDDGLQMFGFTIDCRARMNPPFPAAYFGNCLGGCMAMEDTKQLTGKEGFVTAAKLIGESLHNTLTDKDGLIKDIESFGDLFSSGVPTTVIGVAGTPKLKFYDLDFGWGRPKKIETVSIDYNGSISMNAYRDNYEDLEIGVCLQTTEMESFVRIFEDGLENYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CCW7</accession>
    <name>A0AAD5CCW7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">arginase</fullName>
            <ecNumber evidence="2">3.5.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_014535</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-arginine + H2O = urea + L-ornithine</text>
            <dbReference type="Rhea" id="RHEA:20569"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16199"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="EC" id="3.5.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="8">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Nitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the arginase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.3.1" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010008871">
        <property type="protein sequence ID" value="KAI7738126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CCW7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008783">
        <property type="term" value="F:agmatinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004053">
        <property type="term" value="F:arginase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033389">
        <property type="term" value="P:putrescine biosynthetic process from arginine, via agmatine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11593">
        <property type="entry name" value="Agmatinase-like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.800.10:FF:000007">
        <property type="entry name" value="Arginase 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.10">
        <property type="entry name" value="Ureohydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006035">
        <property type="entry name" value="Ureohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11358">
        <property type="entry name" value="ARGINASE/AGMATINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11358:SF26">
        <property type="entry name" value="GUANIDINO ACID HYDROLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00491">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036979">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51409">
        <property type="entry name" value="ARGINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0056">Arginine metabolism</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 8" id="KW-0464">Manganese</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="8">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="264"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="266"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR036979-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7738126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="320" mass="35259" checksum="0043C51331133A6A" modified="2024-05-29" version="1">MMKAVRRGFHYLHTLNNVPKNLIERGQSRVVEASLTLIRERAKLKGELVRALGGAKASSSLLGVPLGHNSSFLQGPAFAPPRIREAIWCGSTNSTTEEGKELDDPRVLTDVGDVPVQELRDCGIDDDRLMNIISESVKLVMEEDPLRPLVLGGDHSISYPVIRAVSEKLGGPVDVLHLDAHPDIYDRFEGNKYSHASSFARIMEGGYARRLLQVGLRSINKEGREQGKRFGVEQYEMRTFSKDRQFLENLKLGEGVKGVYISIDVDCLDPAFAPGVSHIEPADVVGADVVEFNPQRDTVDGMTAMVAAKLVRELTAKISK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CE25</accession>
    <name>A0AAD5CE25_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_031105</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008490">
        <property type="protein sequence ID" value="KAI7740191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CE25"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.730.10">
        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001471">
        <property type="entry name" value="AP2/ERF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32467">
        <property type="entry name" value="AP2-LIKE ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32467:SF32">
        <property type="entry name" value="AP2-LIKE ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR SMOS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00367">
        <property type="entry name" value="ETHRSPELEMNT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00380">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51032">
        <property type="entry name" value="AP2_ERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="8">
        <location>
            <begin position="82"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="262"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="332"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="262"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="272"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7740191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="440" mass="48537" checksum="C4072EEB0E67764D" modified="2024-05-29" version="1">MATASSSDPVMKSEGNSGGGEVPEAAATTTVAMVGKGSGGTYQQQLMVKYKGLKKSKKERGCTAKERISKMPPCAAGKRSSIYRGVTRHRWTGRYEAHLWDKNTWNQNQNKKGKQGSILSLIVCIHFCYPMLNVSLGAYDDEEAAARAYDLAALKYWGPGTLINFPVTDYTRDLEEMQNLSREDYLASLRRKGSGFSRGTSKCHGVSSTRWDSPLGRIAGADYHSRMHHGDDATTESEYVGGFCMDRKIDLTPYIKWWGPNKSCQTESQGKSSDETNHGSSEDTGSEHKTQEWVTQPTEPYQLPRLGVSPEGKQHKKSSVSAMSILSRSAAYKSLQEKAAKKEAEKDVENDENENKININKMDYGKAVEKSSDDAAPEGFGVGLGIELERNTFPLAPFLSAPLLTNYNTIDPLTDPILWSSLVPSLRTRPSQPVEVSTCY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CET8</accession>
    <name>A0AAD5CET8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein-synthesizing GTPase</fullName>
            <ecNumber evidence="3">3.6.5.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_000027</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.3"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.3" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010008479">
        <property type="protein sequence ID" value="KAI7740258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CET8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005850">
        <property type="term" value="C:eukaryotic translation initiation factor 2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01888">
        <property type="entry name" value="eIF2_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03688">
        <property type="entry name" value="eIF2_gamma_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15490">
        <property type="entry name" value="eIF2_gamma_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000009">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000065">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000075">
        <property type="entry name" value="Translation initiation factor 2 subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050543">
        <property type="entry name" value="eIF2G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015256">
        <property type="entry name" value="eIF2g_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044127">
        <property type="entry name" value="eIF2g_dom_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044128">
        <property type="entry name" value="eIF2g_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003077">
        <property type="entry name" value="PRK04000.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42854">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42854:SF3">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09173">
        <property type="entry name" value="eIF2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="10">
        <location>
            <begin position="42"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7740258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="482" mass="52441" checksum="84C9FB4545F41924" modified="2024-05-29" version="1">MVQNPSATVNMARKAKGLMEQDLSKLDVATLHPLSPEIISRQATINIGTIGHVAHGKSTLVKAISGVQTVRFKNELERNITIKLGYANAKIYKCEDERCPRPMCYKAYGSEKEDSPACDVPGFENSKMKLMRHVSFVDCPGHDILMATMLNGAAIMDGALLLIAANESSLQPQTFEHLAAMEIMRLKHIIILQNKVDLVKENVAINQHETIQKFIQGTVAEGAPVIPVSAQLGANIDVVCEYIIKRIPIPKRNFISPPNMIVIRSFDVNKPGAEFDKIKGGIAGGSILKGVLKVNQHIEVRPGIVVKDESGNIKCSPIYSTIVSLCAEQNELQFAVPGGLIGVGTTMDPTLTRADRLVGQVLGEVGSLPDVYVELEVEFCLLRQLLGVKTNETEKQVRVSKLAKAEVLMLNIGSMSIGARVLAVKSDLAAKKNVAKLQLTSPVCTSKGEKIVLSRRVEKHWRLIGWGTILAGLTLEIPPCPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CEW6</accession>
    <name>A0AAD5CEW6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NADH:ubiquinone reductase (non-electrogenic)</fullName>
            <ecNumber evidence="4">1.6.5.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">M8C21_033515</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">AA19_3_7</strain>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a quinone + NADH + H(+) = a quinol + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:46160"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.6.5.9"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a ubiquinone + NADH + H(+) = a ubiquinol + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:23152"/>
            <dbReference type="Rhea" id="RHEA-COMP:9565"/>
            <dbReference type="Rhea" id="RHEA-COMP:9566"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <dbReference type="ChEBI" id="CHEBI:17976"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Intermembrane side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NADH dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.6.5.9" evidence="4"/>
    <dbReference type="EMBL" id="JAMZMK010008468">
        <property type="protein sequence ID" value="KAI7740303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CEW6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050136">
        <property type="term" value="F:NADH dehydrogenase (quinone) (non-electrogenic) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.100:FF:000002">
        <property type="entry name" value="External alternative NAD(P)H-ubiquinone oxidoreductase B1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045024">
        <property type="entry name" value="NDH-2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43706:SF47">
        <property type="entry name" value="EXTERNAL NADH-UBIQUINONE OXIDOREDUCTASE 1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43706">
        <property type="entry name" value="NADH DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-0274">FAD</keyword>
    <keyword evidence="5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-0520">NAD</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="EF-hand" evidence="16">
        <location>
            <begin position="405"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="17">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="17">
        <location>
            <position position="575"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAI7740303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="575" mass="64700" checksum="003FE88AD255FC60" modified="2024-05-29" version="1" fragment="single">FGISLSLSLSLSKKNPHCESCFSKLSVFACFEAEMKSLNMYERFSNTFRQHPSLSKFLVVFAISGGGVVAYSVANSENYDVVGNQKKKVVVLGTGWAGTSFLRNLKNPSYDVQVISPRNYFAFTPLLPSVTVGTVEARSVVEPIRNIVKKKKVNIDFCEAECYKIDAKNKKVYCRSTKNEKEEFVVDYDYLVVAMGARVNTFNTPGVEENCLYLKEVEDAQKIRRKVIDCFEKASLPNVSDEERKRMLQFVVVGGGPTGVEFAAELHDFVSEDLVKLYPTVKDFVKISLLEATNHILNMFDKRITAFAEEKFHRDGIDLKTGSMVVNVSDKEISTKEIKTGKISTMPYGMAVWSTGIATRPVVLDFMKEIGQANRRVLATDEWLRVEGTNSIYALGDCATINQRKVMEDISSIFEKADKDRSGTLTVKEFQDAVDDICDRYPQVRLYLKNKKMSSLVDLLKESKGDAAKEVNVDELKSVLSQVDSQVKNFPATAQVAAQQGSYLADCFNRMDKCEKNPEGPIRFRESGRHRFRPFRYKHLGQFAPLGGEQTAAQLPGDWVSIGHSSQWLWYSVYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CFK2</accession>
    <name>A0AAD5CFK2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_025778</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008531">
        <property type="protein sequence ID" value="KAI7740030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CFK2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080030">
        <property type="term" value="F:methyl indole-3-acetate esterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080032">
        <property type="term" value="F:methyl jasmonate esterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080031">
        <property type="term" value="F:methyl salicylate esterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009694">
        <property type="term" value="P:jasmonic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009696">
        <property type="term" value="P:salicylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000025">
        <property type="entry name" value="putative methylesterase 11, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045889">
        <property type="entry name" value="MES/HNL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10992:SF1034">
        <property type="entry name" value="ALPHA_BETA HYDROLASE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10992">
        <property type="entry name" value="METHYLESTERASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="3">
        <location>
            <begin position="131"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="74"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="30"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7740030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="380" mass="42119" checksum="53F7F35085B65191" modified="2024-05-29" version="1">MGICLSTKSSPSNKTTRSKRLTNQSTNTHSSTRWSRVRSSSIKREKFDDAVIHEHALAAALLFQQQNGGVLPFDRSSSLRHVPGGSHSKRQQPLLPRSSSTRARSLTDPLLHPQQLLNQDVNVDELEVSHIVLVHGGGFGAWCWYKTIALLEECKFKVTAIDLTGSGIDLFDANSIKSLSQYVTPLTDFLEKLADGEKVILVGHDFGGACISYAMELFPSKIAKAIFIAASMLKSGQSTLDMFSQKENTNDLMRQAQKFLYTNGNNLPPTAIDLDKMLLKDLLFNHSPAKDVALATVSMRPIPFPPVLEKLSLTDANYGSVRRFYIQTPDDNAIPITLQESMINESPPEKVFRLKGSDHSPFFSKPQALHKLLVEITKIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CFM4</accession>
    <name>A0AAD5CFM4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Carotenoid cleavage dioxygenase 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_019502</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="5">Binds 1 Fe(2+) ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the carotenoid oxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008390">
        <property type="protein sequence ID" value="KAI7740624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CFM4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009570">
        <property type="term" value="C:chloroplast stroma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045549">
        <property type="term" value="F:9-cis-epoxycarotenoid dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016121">
        <property type="term" value="P:carotene catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004294">
        <property type="entry name" value="Carotenoid_Oase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10543">
        <property type="entry name" value="BETA-CAROTENE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10543:SF37">
        <property type="entry name" value="CAROTENOID CLEAVAGE DIOXYGENASE 7, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03055">
        <property type="entry name" value="RPE65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="4 5" id="KW-0408">Iron</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="5">
        <location>
            <position position="321"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="381"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="591"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR604294-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7740624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="599" mass="67765" checksum="38106995522A3199" modified="2024-05-29" version="1">MQAKAIFEFIPLKLPSPAIKLPPAQAISFPDHVVRRDISIAIPNQEVSTSVEEEKIAAYWDYQFLFVSQRSETISPISLRVIEGSIPSDFPLGTYYLTGPGLFRDDHGSTVHPLDGHGYLRAFTIDGVKGEVKFMAKYIETAAQVEEHDRETGEWRFTHRGPFSVLKNGEKIGNTKVMKNVANTSVLRWGGRLFCLWEGGAPYEIDSRSLDTIGKFDLINGGDQSSTSSISDRDSRGGGDNMLDLAANILKPILYGVFKMPSKRLLSHYKIDASRNRLLMMSCNAEDMLLPRSNFTFYEFDCNFKILNKQEFTIPEHLMIHDWAFTDSHYILFGNRIKLDILVNPSKSTSPIYLLPRVPEHEGGRDWKVPIEAPSQMWVLHVGNAFQETDHNGNKQIQIQASGCSYKWFNFQKMFGYDWQSGKLDPSMMNVGHSGNKLVPHLIQVSINLDASGNCEECHVNPLNDQWNKATDFPVINNDVGGRNNKYVYAATSSGFRRELPHFPFDTVVKFDTVKNSTHTWSVGTRRFIGEPIFVSKGRDEDDGYLLVVEYAVSKQMCYLVILDAKKVGEKNALVARLEVPKHLNFPLGFHGFWAPMDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-10-08" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CG02</accession>
    <name>A0AAD5CG02_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_029253</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008208">
        <property type="protein sequence ID" value="KAI7741348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009914">
        <property type="term" value="P:hormone transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000032">
        <property type="term" value="P:regulation of secondary shoot formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03233">
        <property type="entry name" value="ABCG_PDR_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03232">
        <property type="entry name" value="ABCG_PDR_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000179">
        <property type="entry name" value="ABC transporter G family member 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000059">
        <property type="entry name" value="ABC transporter G family member 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034001">
        <property type="entry name" value="ABCG_PDR_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034003">
        <property type="entry name" value="ABCG_PDR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013581">
        <property type="entry name" value="PDR_assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48040:SF66">
        <property type="entry name" value="PLANT PDR ABC TRANSPORTER ASSOCIATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48040">
        <property type="entry name" value="PLEIOTROPIC DRUG RESISTANCE PROTEIN 1-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19055">
        <property type="entry name" value="ABC2_membrane_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08370">
        <property type="entry name" value="PDR_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="492"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="523"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="574"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="608"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="633"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="719"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1151"/>
            <end position="1169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1181"/>
            <end position="1199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1220"/>
            <end position="1241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1261"/>
            <end position="1282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1294"/>
            <end position="1313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1325"/>
            <end position="1344"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="121"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="805"/>
            <end position="1058"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="1381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7741348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="1381" mass="155178" checksum="A33B8115F39A05C6" modified="2024-05-29" version="1" fragment="single">MEDAFAMGGGGSHDGRSSRHSMEDEEALRWAALEKLPTYNRLRTTIFKSYVPADDQKHMPTEQMLLDVRELDPQARQGVGITLPTVEVRFQNLSVEADCYVGDRALPTLINAARNIIESLLASVGISFAEKAKLGILKDVTGMIKPGRMTLLLGPPSSGKTTLLLALAGRLDPALRVDGEISYNGHKLNEFEPRRTAAYISQMDIHAGEMTVKETLDFSARCQGVGSRLEMLTELARREKEAGIFPEAEVDLFMKATAIEGDESSLITYYTLRILGLDVCRDTFVGDQMRRGISGGQKKRVTTGEMLVGPAKTLFMDEISTGLDSSTTFQIVKCMQQIAHFSDNTILMSLLQPAPETFDLFDDIILLSEGQIVYLGPREHIVEFFESCGFKCPERKGTADFLQEVTSKKDQEQYWADRHRAYRFIPVSEFSQRFKNFHVGAKLQRELSVPYDKNQSHKAALVYKKYLVPKMELLKASWDKEILLMKRNAPVYVFRTIQIVFISFICTTLYLRTTMHERNEIDGSLYVGALLDSMLINMFNGLADLSFTVMRLPVVYKQRDLMFHPPWAFTLPAFLLRIPMSMIESVMWTVLLYWGVGFAPDASRFFKHLLLVFLIQNVAAGLFRLIAGVCKTMSMANTGGSVVLLLIFLLGGFILPKTQLPNWWEWAYWLSPLSYIFKSITINEFLDSRWTGKNSTDGTTNLGFAVLKALDVPTSESSYWIGAGALVGFIVLFNILFTVSLMYLEAPGKPQAIISKAEAAAMNGHAAEGDDSRGNKKMRQFSSSISNTNGASKKGMVLPFTPLAMSFNDMNYYVEMPSEMREEGVTDNRLQLLRDVTGAFRPGVLTALMGVSGAGKTTLMDVLAGRKTGGYIEGDIRISGFPKKQETFARISGYCEQTDIHSPTITIHESLIYSAFLRLSKDVSKEDKMLFVSEVMELVELDNIKDAIVGLPGVSGLSTEQRKRLTIAVELVANPSIIFMDEPTSGLDARAAAIVMRAVRNTVDTGRTVVCTIHQPSIDIFESFDELLLLKRGGQVIYSGPLGRHSQQIIEYFEAIDGVPKIPEKYNPATWMLEVSSGAAEMRLGIDFAEHFSASSMQQRNKALVKELSVPPPGATDLHFDTQYAQSSWGQFKSCIWKMWWSYWRNPDYNLVRFAFTFLCAIMVGTVFWKIGNKRSNGSDLNAVIGAMYSAIFFVGINNSQTVQPVVATERTVFYRERAAGMYSSLPYAMAQVLVEIPYAFAQTVYYSLIVYSMVSFEWTAAKFFWSFFLNFFSFLYFTYFGMMTVSITPNEQIAAIFAAGFYLLFNIFSGFYIPQPKIPGWWIWYYWICPMAWTVYGCIVSQYHDADNQIFVLGQTGNQTMAEYINDYYGFELDFMGPVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CGC6</accession>
    <name>A0AAD5CGC6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_014906</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="5">Protein modification.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008276">
        <property type="protein sequence ID" value="KAI7741097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CGC6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd23792">
        <property type="entry name" value="UBCc_UBE2D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000001">
        <property type="entry name" value="Ubiquitin-conjugating enzyme 28, E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="9">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="6">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00043952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7741097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="148" mass="16703" checksum="C7B928B43B9BC8B0" modified="2024-05-29" version="1">MASRRISKELKDLQRDPPASCSAGPVAQDMFHWQATIIGPNDSPYAGGVFQVTIHFPPDYPFKPPKVAFRTKVFHPNINNNGNICLDILKDQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKIDKVKYEAMARSWTQKYAMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CJ15</accession>
    <name>A0AAD5CJ15_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">WAT1-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_019427</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007971">
        <property type="protein sequence ID" value="KAI7742507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CJ15"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030184">
        <property type="entry name" value="WAT1-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31218">
        <property type="entry name" value="WAT1-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="79"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="107"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="136"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="181"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="213"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="249"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7742507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="278" mass="31155" checksum="8922FEAD01757694" modified="2024-05-29" version="1" fragment="single">VQRRKYSTLFNIGDDGSIPSLTFSLTLEKTNMATERSDETWSWMKEVMPFVAMLMVTCLDMSVLTIVKAAMNDGMSSIVYIVYHDVLAILILLPFFIIGFFRNGDRPPLSLHLLFRLFVLGFLGMERIEIKSSSTIAKLSGTIIAIFGAMLFTLYQGPEIFHMSPFSHSSNQVHLSRPSNWIFGGLILIVGGIFNAIWKTLQSATAREYPDQRTMVFFYCLFGTIQCIAISPFLEPNPQAWVLQPGTEIIAIILGAVFSVVFRSTVITWCLEKKGPGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CKR9</accession>
    <name>A0AAD5CKR9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein IQ-DOMAIN 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_002222</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the IQD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007698">
        <property type="protein sequence ID" value="KAI7743716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CKR9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32295">
        <property type="entry name" value="IQ-DOMAIN 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32295:SF134">
        <property type="entry name" value="PROTEIN IQ-DOMAIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="18"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="37"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7743716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="271" mass="31193" checksum="EA45BACC31EFA095" modified="2024-05-29" version="1">MGSCGWVKRLFGVKRKKENRPEIGEIQQDSRNSNGSRGRDSSVRESSFRVGSTKVIGRSTGTKEFAAIRIQTAYRSYRARKALRCLKGTSRFQEIIEADALTKQASSALNKIHFWSKIQTEIRNRRHWMVAEGRMKQKKFENQVKFESKLHELEVEWCGGPETMEEIVSRMQQREEATNKRERAMAYAFYHQWRANSNRYFGQAYYDLSKESWGWSWKERWIAVCPWEARVVVQAKPGKKTKHGVGGAPKIVVAVKPHVSTGKGTTIKGTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CMJ2</accession>
    <name>A0AAD5CMJ2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_030863</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007762">
        <property type="protein sequence ID" value="KAI7743256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CMJ2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048188">
        <property type="term" value="C:Set1C/COMPASS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042800">
        <property type="term" value="F:histone H3K4 methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044570">
        <property type="entry name" value="Set1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45814">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SETD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45814:SF2">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SETD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7743256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="103" mass="11500" checksum="41E9FD2B7FAB1FF0" modified="2024-05-29" version="1" fragment="single">PRIALPISSRNWVTDPSSPFTSRVTLTQPSFTASVTITQSPFSTPVTVTQPSFSALYLTQRFFSADYQAEDAHVARIFSGEEITYNYKFPLEDNKIPCNCGSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CQ40</accession>
    <name>A0AAD5CQ40_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Sm domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_031837</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of LSM protein complexes, which are involved in RNA processing. Component of the cytoplasmic LSM1-LSM7 complex which is involved in mRNA degradation by promoting decapping and leading to accurate 5'-3' mRNA decay. The cytoplasmic LSM1-LSM7 complex regulates developmental gene expression by the decapping of specific development-related transcripts. Component of the nuclear LSM2-LSM8 complex which is involved splicing nuclear mRNAs. LSM2-LSM8 binds directly to the U6 small nuclear RNAs (snRNAs) and is essential for accurate splicing of selected development-related mRNAs through the stabilization of the spliceosomal U6 snRNA. Plays a critical role in the regulation of development-related gene expression.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the snRNP Sm proteins family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007044">
        <property type="protein sequence ID" value="KAI7746116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CQ40"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120115">
        <property type="term" value="C:Lsm2-8 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01730">
        <property type="entry name" value="LSm3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000007">
        <property type="entry name" value="U6 snRNA-associated Sm-like protein LSm3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034105">
        <property type="entry name" value="Lsm3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040002">
        <property type="entry name" value="Sm-like_LSM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13110">
        <property type="entry name" value="U6 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Sm" evidence="10">
        <location>
            <begin position="13"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00054684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7746116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="99" mass="11355" checksum="E953CA4E5756DEDB" modified="2024-05-29" version="1">MATATEEESAVKEPLDLIRLSLDERIYVKLRSDRELRGKLHAYDQHLNMILGDVEEIVTTVEIDDETYEEIVRSTRRTVPFLFVRGDGVILVSPPLRTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CQ78</accession>
    <name>A0AAD5CQ78_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_006209</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007515">
        <property type="protein sequence ID" value="KAI7744516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CQ78"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7744516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="85" mass="10437" checksum="C1EA367C8B776EC1" modified="2024-05-29" version="1" fragment="single">LDVYVFLSIWDLKTYLIYYQIRYLVTQSRRWSRGLRLRTELRGINKQISFWNSTHHPYLQQKSQLTEFDQVMFDAGLLMKLLNDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CQA1</accession>
    <name>A0AAD5CQA1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Serine/threonine/dual specificity protein kinase, catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_017607</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007012">
        <property type="protein sequence ID" value="KAI7746283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CQA1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009506">
        <property type="term" value="C:plasmodesma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004714">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000158">
        <property type="entry name" value="Calcium-dependent protein kinase 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000039">
        <property type="entry name" value="receptor-like protein kinase FERONIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045272">
        <property type="entry name" value="ANXUR1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27003">
        <property type="entry name" value="OS07G0166700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27003:SF361">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="22"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="374"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="407"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="455"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7746283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="510" mass="57352" checksum="CA33D1069AA51E9D" modified="2024-05-29" version="1">MSALKESQHLKIPLKDIEIATNNFQHCIGIGGYGNVYKGELLIHGKSTIVAVKRLNEQFGQGLKEFLTEIQLLSGQNHPNLISLLGYCDEGKEKIIVYEYAANGSLDQYLRRGSNVPALTWMERLIICVDAARGLNHLHHEVGKHQTIIHRDIKSSNILIDNNWVAKISDLGLSKLGLSGLNRSAVISRVCGTPGYCEPEYFSTGIVTKESDVYSFGIVLFEVLCGKLCCTVDHDGFQLSATVVKDYYKKKKVSEIVDPILKEQMSSELIRDFSKIAYRCLHEDRDQRPPMTLVVKKLERLLAIQDVLLKAAESTLSDVIKPAHPLVREGGNASEIPLDISVLSNMRDFVKYNRLKQFALRALASTLDEDELWHLKDQFYAIDVDKNGAISLEEMRDAFAKDLPWKLKETRVLEILKAIDSNADGLVDFTDFVAATLNVYQLEGQDSERWQILSQAAFEKFDVDRDGYITPEDIRMHTGLKGSIDLLLKEADIDKDCKISLSDFCRLLGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CQI9</accession>
    <name>A0AAD5CQI9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">ADP-ribosylation factor 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_027070</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007403">
        <property type="protein sequence ID" value="KAI7744949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CQI9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016004">
        <property type="term" value="F:phospholipase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003500">
        <property type="entry name" value="ADP-ribosylation factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11711">
        <property type="entry name" value="ADP RIBOSYLATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 12" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0519">Myristate</keyword>
    <keyword evidence="5 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="3"/>
            <end position="10"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="106"/>
            <end position="109"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00040200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7744949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="161" mass="17964" checksum="FCC162A5CF11AE08" modified="2024-05-29" version="1">MAGLGGAGKTTILYSLKFGKVVSTTVTIGFNIETLEYKNIRFEVWDIGAYNMIHLRLRRHFTQNVQGLIFVVDSVDRDHVNEAKEELHLLFKEDGLRDALLLVFANKQDLPGAMNATEIVDKLDLHSLGLQHWHILSGSATTGEGLYEGLDWLANNIANKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CR21</accession>
    <name>A0AAD5CR21_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Actin-related protein 2/3 complex subunit 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_011901</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Functions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the Arp2/3 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cytoplasm</location>
            <location evidence="1 6">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the ARPC3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007156">
        <property type="protein sequence ID" value="KAI7745695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CR21"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005885">
        <property type="term" value="C:Arp2/3 protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034314">
        <property type="term" value="P:Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030833">
        <property type="term" value="P:regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1760.10">
        <property type="entry name" value="Actin-related protein 2/3 complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007204">
        <property type="entry name" value="ARPC3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036753">
        <property type="entry name" value="ARPC3_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12391">
        <property type="entry name" value="ARP2/3 COMPLEX 21 KD SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04062">
        <property type="entry name" value="P21-Arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016315">
        <property type="entry name" value="ARP2/3_P21-Arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69060">
        <property type="entry name" value="Arp2/3 complex 21 kDa subunit ARPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR016315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7745695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="174" mass="19447" checksum="3499A1F2FCEC657C" modified="2024-05-29" version="1">MVYHSTFVDDEGITKACGCPLLPLKSHIRGPAPVSDQATTDIIDEAITFFRANVFFKNFDIQSPADKLLIYLTFYINVALKRLEGCRTLAEGTKAVINLGLEDVPVPGESGFPFPGLFPLPVSQQEAELLRNYLKQIREETSGRLLSVAYRPNGTPNKWWLAFAKRKFINTIAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CSE5</accession>
    <name>A0AAD5CSE5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">MORF/ORRM1/DAG-like MORF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_020292</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006857">
        <property type="protein sequence ID" value="KAI7747032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CSE5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016554">
        <property type="term" value="P:cytidine to uridine editing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080156">
        <property type="term" value="P:mitochondrial mRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039206">
        <property type="entry name" value="MORF/ORRM1/DAG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054059">
        <property type="entry name" value="MORF/ORRM1/DAG-like_MORF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31346">
        <property type="entry name" value="MULTIPLE ORGANELLAR RNA EDITING FACTOR 2, CHLOROPLASTIC-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31346:SF4">
        <property type="entry name" value="MULTIPLE ORGANELLAR RNA EDITING FACTOR 8, CHLOROPLASTIC_MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21864">
        <property type="entry name" value="MORF_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="MORF/ORRM1/DAG-like MORF" evidence="3">
        <location>
            <begin position="55"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="13"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="169"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="13"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="169"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="204"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="259"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="286"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF21864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7747032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="327" mass="36642" checksum="C95F1301AEF2D1C2" modified="2024-05-29" version="1" fragment="single">AAIASAATNIRHSPVPTTDVRTFSSRPTTSSLNDSNPNWSNRPPKETILLDGCDFEHWLVVVEKPEGDPTRDEIIESYINTLAKVVGSYDEARMKIYSVSTRCYYAFGALVSEELSYKLKELPGVRWVLPDSYLDVKNKDYGGEPFIDGKAVPYDPKYHEEWIRNNARANERNRRNDRPRSNDRSRNFDRRRENMQNNRGPPNMDGPPPNMRPQGPPSPPYNRGPQGPPPPPPPYYAGPQGPPPPPPPYNAGPPNTRPQNTGEGMPNYQQQGQYGPNSGWMPNNAPSYQQQGQYQSGPGPNSYPPNQNPYQPQDVPGRDMPAAANYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CT33</accession>
    <name>A0AAD5CT33_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Trafficking protein particle complex subunit 2-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_030595</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAPP small subunits family. Sedlin subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006994">
        <property type="protein sequence ID" value="KAI7746344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CT33"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14854">
        <property type="entry name" value="TRAPPC2L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006722">
        <property type="entry name" value="Sedlin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044760">
        <property type="entry name" value="TRAPPC2L"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12403">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04628">
        <property type="entry name" value="Sedlin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7746344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="101" mass="11427" checksum="3C7A9157D1460995" modified="2024-05-29" version="1">MIVCIAVVGHQNNPLYIQSFTEADDALKLHHIVHCSLDVVDERVNNPKKSGPMLNETFLGLLYPTENYKVYGYLTNTKVKFILVTTDLDVRDADVRNVSAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CT44</accession>
    <name>A0AAD5CT44_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_007712</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006894">
        <property type="protein sequence ID" value="KAI7746805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CT44"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027949">
        <property type="entry name" value="Chloroplast_duf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33358">
        <property type="entry name" value="F-BOX PROTEIN WITH A DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33358:SF12">
        <property type="entry name" value="F-BOX PROTEIN WITH A DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14476">
        <property type="entry name" value="Chloroplast_duf"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7746805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="136" mass="15333" checksum="282B03FB6A5AB962" modified="2024-05-29" version="1" fragment="single">MLEKFPAKVEPAVWWPEKRRPSAKRQQDKNGWSVELEEEMREIIKVLEVKDKDDYLRLGEKALKLNKGLAIAGPLLTGLGAIGSGESIESNLDERDVERRENGEVFEMKVALQLGRSLSELRDVAASSSRKGKDIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CTH6</accession>
    <name>A0AAD5CTH6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_027892</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006619">
        <property type="protein sequence ID" value="KAI7747968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CTH6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="2">
        <location>
            <begin position="7"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7747968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="190" mass="22431" checksum="748472B69B8CB0BD" modified="2024-05-29" version="1">MFLQHKYYVFVKNLPFFSSVTSLEQYFTSFGDVEKCILIDNGRACYTRTAKVKMVSKKDVKKILKQEKHYLDGKQIQVMKYFGMATHKAEDVEGKYHLLFDKLIDPNLVSISRWEENNDQGEVMTRELEDAFHQFEYTRIHPFRNDDFLYTGYAQVTFKDSGNASQILAVAQSEFGDRFRRAWDEDEDLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CUM8</accession>
    <name>A0AAD5CUM8_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_012408</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006496">
        <property type="protein sequence ID" value="KAI7748608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CUM8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030267">
        <property type="term" value="F:glyoxylate reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016618">
        <property type="term" value="F:hydroxypyruvate reductase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12156">
        <property type="entry name" value="HPPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000213">
        <property type="entry name" value="Putative 2-hydroxyacid dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050223">
        <property type="entry name" value="D-isomer_2-hydroxyacid_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029752">
        <property type="entry name" value="D-isomer_DH_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10996">
        <property type="entry name" value="2-HYDROXYACID DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10996:SF270">
        <property type="entry name" value="GLYOXYLATE_HYDROXYPYRUVATE REDUCTASE HPR3-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00065">
        <property type="entry name" value="D_2_HYDROXYACID_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase catalytic" evidence="5">
        <location>
            <begin position="5"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding" evidence="6">
        <location>
            <begin position="31"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7748608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="238" mass="26182" checksum="DA9AF0B825796343" modified="2024-05-29" version="1">MADNLPNCKQRGVKVANAGSIFSEDVADAAVGLLIDVLRRVSAANRFVKAGLWPQNGDYPLGHKLSGRRVGIVGMGSIGLDVAKRLDAFGCIILYTSRNNKPHVKFPFYPNIQELAAKCEILVICCALTDQTRHMVDKSVMLALGRDGIIVNIARGAIINEKDLVECLLKQEIAGVGLDVFEDEPNVPEELFELDNVVMTPHHAVMTEESMRNLYELVVGNLDAFFSNKPLMFEVMQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CW84</accession>
    <name>A0AAD5CW84_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_001858</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006348">
        <property type="protein sequence ID" value="KAI7749493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CW84"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042158617" evidence="1">
        <location>
            <begin position="19"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7749493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="99" mass="11758" checksum="EADDE8B2409A2B74" modified="2024-05-29" version="1">MANLMLLFHLCPLPGTFGRFGWRQNARGEVMRIQSAMAICRLGCNARARKELGEYGFILPLIMMWKVDRLKRKRRLQRRCQRSWFIKGIKGFTGWKREV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CYA8</accession>
    <name>A0AAD5CYA8_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_030145</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006321">
        <property type="protein sequence ID" value="KAI7749632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CYA8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7749632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="99" mass="10879" checksum="05C8E399CB4439DD" modified="2024-05-29" version="1" fragment="single">KIQAIMDLITIVAYMWNGLGGQGRMDDEIPLKCINGSPDLRPGISQSTNRISDIDDDDAKHDEMLMDAILDLLPAFAKVMGSHFAPIFAALFVPLMKFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CZ92</accession>
    <name>A0AAD5CZ92_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Protein CHROMATIN REMODELING 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">M8C21_020508</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">AA19_3_7</strain>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005982">
        <property type="protein sequence ID" value="KAI7751103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140658">
        <property type="term" value="F:ATP-dependent chromatin remodeler activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18660">
        <property type="entry name" value="CD1_tandem"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18659">
        <property type="entry name" value="CD2_tandem"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15539">
        <property type="entry name" value="PHD1_AIRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11660">
        <property type="entry name" value="SANT_TRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000607">
        <property type="entry name" value="chromodomain-helicase-DNA-binding protein 1-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009463">
        <property type="entry name" value="DUF1087"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45623">
        <property type="entry name" value="CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45623:SF32">
        <property type="entry name" value="DNA HELICASE CHROMATIN REGULATOR PHD FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06465">
        <property type="entry name" value="DUF1087"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01147">
        <property type="entry name" value="DUF1087"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="15">
        <location>
            <begin position="72"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="Chromo" evidence="14">
        <location>
            <begin position="435"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="domain" description="Chromo" evidence="14">
        <location>
            <begin position="507"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="16">
        <location>
            <begin position="607"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="17">
        <location>
            <begin position="912"/>
            <end position="1071"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="129"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="378"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1080"/>
            <end position="1118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1316"/>
            <end position="1335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1349"/>
            <end position="1370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1402"/>
            <end position="1422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1604"/>
            <end position="1624"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1798"/>
            <end position="1954"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="37"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="51"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="146"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="195"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="237"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="269"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="283"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
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            <begin position="317"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="378"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="398"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1088"/>
            <end position="1105"/>
        </location>
    </feature>
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        <location>
            <begin position="1317"/>
            <end position="1333"/>
        </location>
    </feature>
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        <location>
            <begin position="1608"/>
            <end position="1618"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="1876"/>
            <end position="1885"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1930"/>
            <end position="1941"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1943"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAI7751103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="20">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1954" mass="218266" checksum="CF07D5FA6C5259D2" modified="2024-05-29" version="1">MKNNETSASDVINGNWTLKRKRKKIGAAKSNGKKNESTPSDSHTSTSSKCKIKEESSSDRSPSKKKRKDGSYHECVICDLGGDLLFCDGCSKTYHIACLDPPLKQRPSDTWQCPSCCSESNVVEAVDKVESTSKRARTKVTVGKPKSKDKSAKTDKVSRILRNTNLGTKKSTRKTKSLFASPKGDGSPERSSSFSDHHNEKQTDLGEKEVIKNKKKRDRDQSKETATGKSEKKRKNISSEDVQKRNRTDSARKRDKVSLEVNETSKSQQQKHKSVKHAKTKSLSKSGMGSKNSDIRSKDKKKIGAKPSESQEEEKDVELLKNSDKTQQVDRVLGCRIQVTEKQSAVSVIEADKSDNTNGEPEKNTTIFSIRDVVNECTEGNATGSTGKPTSDPNSLETDERDKDDSALKSNDLTKPCKEIVNGDSTDVIIDDTKTDLEKSANCSKSLSNNEKTTVSYEFLVKWVGKSNLHNTWVSESQLKILAKRKLDNYKGKYGTMVINICEERWKVPQRIIAQRSSADGSTEVFVKWTGLPYDECTWEKTDEPAIEKSSYLIDLFKRFETQAVEKDNFKDEVLRGKGEVTTLTEQPEELGGSLFPHQLEALNWLRKCWHRGKNVILADEMGLGKTISACAFLSSLYFEFKARLPSLVLVPLSTMPNWMSEFNMWAPNLNVVEYHGCARARALMRQYEWHANGTKNKKTSSYKFNVLLTTYEMVLADSSHLSGVPWEVLVVDEGHRLKNASSKLFSLLNTFSFQHRVLLTGTPLQNNIGEMYNLLNFLQPSSFPSLSAFEDRFNDLSTAEKVDELKKLVAPHMLRRLKKDAMQNIPPKTERIVPVELSSIQAEYYRAMLTKNYQVLRNIGKGIPQQSMLNIVMQLRKVCNHPYLITGTEPETGTVEFLHDMRIKASAKLTLLHSMLKILKKEGHRVLIFSQMTKLLDILEDYLNIEFGPKTFERVDGSVSIADRQMAIQRFNTDKSRFVFLLSTRSCGLGINLATADTVIIYDSDFNPHADIQAMNRAHRIGQSNRLLVYRLVVRASVEERILQLAKKKLMLDQLFVNKSGSQKEVEDILRWGTEQLFNDSSTQSGKDGENNENKGDESVITEHKSKRRTGGLGDVYQDKCTDGNSTIVWDESAILKLLDRSNLEHISSDNGEGDQENDMLGSVKSVDWNDESTEEQGGSEALVDVTDDATAQNLEKKDDHDSSGNVAEENEWDRLLRVRWEKYQSEEEAALGRGKRQRKAVSYREAYAPRPIETFENGGSEEPEPQPMREYTPAGLALKTKYSKLRARQKLRLAQMKAFKESLLNGGSDLMWISDQPTKENPSTEDNQTSKVQKRNFPTVLGLCAPNSTLMESSQKNASKSNKKNKHGKSLEFPFHLAPCSEAGTSTDVDAKPNGAIANRTSVPPFNPHVASESSGDGLPHSHEKMVLPRLPFDESFLSKLPFPVKNFGQHQVDYFPRLSLGRSTGGESSASLQDFLAIPFLPNFRLPQQEMEALTLPKMGLDQMPPSSSSLSENHRKVLENIMMRAGSGPGLGNAVNKRKPVQDFWSEEELDFLWIGVRRHGRGSWETMLRDPRLKFSRFRTWEDLAARWEEEQVKVLDIRPQKKSNGSKSSLKSPGFPNISDGMMKRALNRSRFAVGPPSFPPHLMDMKLALDGPSASMESPDQHGFQHDGFPPFPMRIPDRFRAGESSTERVIPNPFSIGSLDLNGLGGLDLQQKEVIHVNDRAKIPNFVDNASRSKNEVGASSASDNNKLPHWLREAVSVPAHQPQEPQLPPTVSAIEESVRLLYGDEKRTIPPFIAPGSLPLPPKDPRQNLKKKKKSSSSSNSSSHGVLHDQPSLEDKQVASTSGSKAAPPPEHLQSTSEPSWTEPDLNAPPPPPSPPVNQDMAVSPQLPSLNSDPDIATPMEMEPKQKESVSEGSGRGKSKLNPTTQDASSEETALDHGEGDGESC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CZZ5</accession>
    <name>A0AAD5CZZ5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_012564</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005989">
        <property type="protein sequence ID" value="KAI7751079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CZZ5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000028">
        <property type="term" value="P:regulation of photoperiodism, flowering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039319">
        <property type="entry name" value="ELF3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34281">
        <property type="entry name" value="PROTEIN EARLY FLOWERING 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34281:SF2">
        <property type="entry name" value="PROTEIN EARLY FLOWERING 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="167"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="288"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="167"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="288"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="311"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="324"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="354"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7751079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="428" mass="47287" checksum="9CB4B7B0DB21ED93" modified="2024-05-29" version="1">MCHDYNIYRLADEGDRDTRRQKTMIILAHRQPQATIAVIYFLILRIQNPMRLEQLKVTDLLIAKHAKMKVVGVVRVTSIEISERDYASRVDLIAKVQRLIAEPPQLLVDENGYLAKSPKVSTIKKLPFEYVMKLTVNIPKHKNDIEKLNDDMEFSYENAVGKASLSTVQNGSQTTNCRPFGGTPLPPSDQNMGSWNFNAPPGYQWLIPVMSPSEGLVYKPYPASWFLSPAYGGCGPPGSMPVIGPNFANYGAPPSHYEGFTGVNPFNPPLLSSHGYFPPYGVQMANPSVSSSARFEPSNPTNQGSTNILVEETRPTVPKVDRLNNNNNKNNNNNDNNNNTASKDTEVQASTARSRSDRSKDRNALPRFPTSPPPAPAPATALEPVRVKAACPSRVIKVVPHNAWSATASVARIFKSIQEERKQQYGSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D179</accession>
    <name>A0AAD5D179_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_018767</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005951">
        <property type="protein sequence ID" value="KAI7751200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D179"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31818">
        <property type="entry name" value="O-FUCOSYLTRANSFERASE 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31818:SF3">
        <property type="entry name" value="O-FUCOSYLTRANSFERASE 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="103"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7751200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="112" mass="12852" checksum="5AA85BCE6E7BFF88" modified="2024-05-29" version="1">MGHKRTIRPNARKLNALFSQREKMPWTTFSRKVKSAQREFMGEPDDIKPGRTEFHEYPSSCICKKPLNFSLPDNKRITTNASSYGHNEGVNSRDNDASSSLEEKEDDDFLAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D2F0</accession>
    <name>A0AAD5D2F0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">H15 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_003043</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006077">
        <property type="protein sequence ID" value="KAI7750846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D2F0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031492">
        <property type="term" value="F:nucleosomal DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030261">
        <property type="term" value="P:chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045910">
        <property type="term" value="P:negative regulation of DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005818">
        <property type="entry name" value="Histone_H1/H5_H15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11467:SF109">
        <property type="entry name" value="H15 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11467">
        <property type="entry name" value="HISTONE H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00538">
        <property type="entry name" value="Linker_histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00526">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51504">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="H15" evidence="5">
        <location>
            <begin position="77"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="153"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="263"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="395"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="466"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="595"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="670"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="775"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="9"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="156"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="195"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="223"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="395"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="483"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="517"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="675"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="686"/>
            <end position="704"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7750846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="807" mass="91107" checksum="F51F393C182B30A6" modified="2024-05-29" version="1">MDPTTVQPPQSPPPPPLNPDTPTDAVIEPSDAPSSRRNMNNFKALVFKLAGRFSPVNQSLIQRRFDDIIPQELPVPDHPPYDDMILTALWELKAEGGSSEEDISHFIVKEYHELPWAHATILNYHLRDLCKKRKITMTNDRYHLSCLSINKPEPISSPSPCSTPTSTSCSSSSTNICTSPSPSSSSSSLSWGGSNRRRKRENIKGRRGKIVQVSSSDSDWQRSRGRGGGRGKGNGRGRVRGRGTEVGQRRNVKDQFVVEVSDEVEETNHETEDQNEVTSMEVEDDSIKDGDLPDVIAKVSEEENLPCVQNNKEQSRPKKMYNVTFIGRRSRKLTKEEIDFLNGKFDDEVTELEADVGDAEVDEHAEQPEQKEAEIDTLNLQSDHEVTVLEVDVGDAKVDEHAENLEQKEEKGAIDMEVEGERQEENQQSDTVERQTLGEERVMDDENPSEEQSSVMIQCEAQVTVASVEKHHGDAFEEQIQPEENKEERVEKTEESDKQLDCLTKEQVETKAQSEDLHDDDDDVTEEPILPQELCVGKAEEFDKQLDHLSKEQVETKAQSADLHDDDIVAEEPIHPHEECVEMTVESDMQLDFSTKEVETKAQSADLHDDVTEEPIHRQEQRVEKTEEYLRACEENQLKNVEAESAEREHQTEVNQKEIVLAEVQQEPFVEITEQHNQPCIQQTETSEKDSLQEKTNPVRKEENTTSSGSTSEGSRRPLRQGLRSSSKKEDAVPKSNSKKQLELVTPVRRSLRSNTTISEPAVVTEVLDQSKELVANSGRTRSSKRKTKIAEAEQVSNTRKTRRTGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D2F2</accession>
    <name>A0AAD5D2F2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Hexosyltransferase</fullName>
            <ecNumber evidence="13">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_004160</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2 13">Glycan metabolism; pectin biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Golgi apparatus membrane</location>
            <topology evidence="1 13">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the glycosyltransferase 8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="13"/>
    <dbReference type="EMBL" id="JAMZMK010005582">
        <property type="protein sequence ID" value="KAI7752926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D2F2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047262">
        <property type="term" value="F:polygalacturonate 4-alpha-galacturonosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06429">
        <property type="entry name" value="GT8_like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000056">
        <property type="entry name" value="Hexosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029993">
        <property type="entry name" value="GAUT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002495">
        <property type="entry name" value="Glyco_trans_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32116">
        <property type="entry name" value="GALACTURONOSYLTRANSFERASE 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32116:SF104">
        <property type="entry name" value="HEXOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25557">
        <property type="entry name" value="GAUT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01501">
        <property type="entry name" value="Glyco_transf_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 13" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 13" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9 13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="16"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU362027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7752926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="544" mass="62487" checksum="57C39BD2DFA81CFC" modified="2024-05-29" version="1">MRRRAADYRRRVRRRLSIWIWLFLVLFSVAGCVLIFVQHNFQDHDHGQVQFQDQDRQTVLDRDARNIQVTHNRLNLTKEIGSANSYARQLLEQMTLAKAYVVIAKEHNNLHLAWQLSSKIRNCQSLLSKASMRADPITQEEAEPLIISLSSLILKSQDAHYDLATTMMTMKSHIQALEARANAATVQSAYFGNLAAGSLPKNHHCVNVKLVADWITNKRIKELADENNNSPRLSDNNLYHFCLFSDNLMAISAVVNSTISNADHPKQLVFHIVTNGANYAAMQAWFVTNDFKGSMIEIQKVEDFTWLNASYSPVVKNFKKVDFFGYPYEDSQDSEHDPKFLNEKYESLLNHLRFYIPEIYPHLEKVVFLDDDVIVQKDLTPLFSLDLHGNVNGAVETCLEAHHRLYKYLNFSNPVLSSKIDPQGCGWAFGLNVFDLVAWRNANVTDMYHYWEEQNADGNLWKLGTLPAGLLAFYGRTEPLDRRWHVLGLGFDVNIDSRLIESAAVVHFNGNMKPWLKIGIGRYKPLWQRYVDRNHPYILACATS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D2U8</accession>
    <name>A0AAD5D2U8_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_008707</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006005">
        <property type="protein sequence ID" value="KAI7751056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D2U8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7751056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="98" mass="11083" checksum="7C64954B072CFF8C" modified="2024-05-29" version="1" fragment="single">VANIGTMNRSRESPTPRLQPRPDSSQSHIRAQLSFYPDFVSVHRLASLIRSILSSEMTITISIEDVRTEGGRYIEVDAKLIITQILSVEKARDKEEND</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D357</accession>
    <name>A0AAD5D357_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ankyrin repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_015120</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005722">
        <property type="protein sequence ID" value="KAI7752212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D357"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24121">
        <property type="entry name" value="NO MECHANORECEPTOR POTENTIAL C, ISOFORM D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24121:SF16">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7752212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="260" mass="29072" checksum="75FDCC93316F01B2" modified="2024-05-29" version="1">MNKVRINSFNNRQQQPQQKNHTVVSIPTLPVAPPKPNLPRSDLFEGSREDYVKLGIPLYNAAIKGDWKAAKCILEKQPDLTSFAITENHETLLHIAAGAECTKSVVEFVTKLVQLMECKDLELQNKNYNTAFSIAAAAGNVKTAMIMVNKNPAVAEIPGNNRTMPLYMAALFAKPEMARYLYSISKKMGGDYWSHDNRGWVLQKCVEAEIFDVAIKIVNDRPELLDKKGLLTDVLIALALKTQINVFHSITESSNFDPRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D4H2</accession>
    <name>A0AAD5D4H2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_029222</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the major facilitator superfamily. Phosphate:H(+) symporter (TC 2.A.1.9) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005635">
        <property type="protein sequence ID" value="KAI7752689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D4H2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11654">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="43"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="80"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="104"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00044504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7752689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="121" mass="13266" checksum="4C38B4B2DB2DC19E" modified="2024-05-29" version="1">MEFNEMQKDVKEENGSQHMAIEGRNNGFIKHSKRGGGWTTFPFILGGMLSLTLTAGGWIGNLIVYLITKYNVKSIDATQVFNVVVGCMSLFPIVAAVLSDSFCGSFTVVTIFSFVSLLVYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D4S0</accession>
    <name>A0AAD5D4S0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_006446</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010004110">
        <property type="protein sequence ID" value="KAI7754216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D4S0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="16"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7754216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="80" mass="8080" checksum="514C3A117A2F15B9" modified="2024-05-29" version="1" fragment="single">TIAVSPVPSIPLVTSSAVEEAANPAEREGIGGDGGGSSGGAAFVTSHSYGGIRVMGLKELLEAGNRERVVVGGGAWWWWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D5I2</accession>
    <name>A0AAD5D5I2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">gibberellin 3beta-dioxygenase</fullName>
            <ecNumber evidence="9">1.14.11.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_020632</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Hormone biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7">Plant hormone biosynthesis; gibberellin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the iron/ascorbate-dependent oxidoreductase family. GA3OX subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.15" evidence="9"/>
    <dbReference type="EMBL" id="JAMZMK010005508">
        <property type="protein sequence ID" value="KAI7753214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D5I2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016707">
        <property type="term" value="F:gibberellin 3-beta-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009686">
        <property type="term" value="P:gibberellin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.330:FF:000013">
        <property type="entry name" value="Gibberellin 3-beta-dioxygenase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050231">
        <property type="entry name" value="Iron_ascorbate_oxido_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47990">
        <property type="entry name" value="2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="6 10" id="KW-0408">Iron</keyword>
    <keyword evidence="3 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="12">
        <location>
            <begin position="191"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="148"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00061560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00066695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7753214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="357" mass="39618" checksum="91EAE52D8F3F4891" modified="2024-05-29" version="1">MHKRYPMINHKYLDLTSMKELPESHAWLSLDHELPSHNSSTPDQVPVINLEDPNAMKLVGHACKTWGVFQVTNHGVPTNLLQQMEAAGRKLFALPMQQKLKAARSPNGVSGYGVARISAFFPKLMWSEGFTIVGSPYEHARKLWPNRYSRFCDVIEEYKEEMNKLAQKLMSLMLGSLGVTMEDVKWATTQRSCPALQLNSYPACPDPDRAMGLAAHTDSTLLTILHQNNTSGLQAHKEGTGWVTVPPIPGALVINVGDLLHILSNGLYPSVLHRAMVNRTQHRLSVAYLYGPPSNVEISPLSKLTDQVHPPLYRAVTWSEYLGTKAKHFNKALSSVRLCGPLNGFADTNDHNGVQVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D6A2</accession>
    <name>A0AAD5D6A2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Proteasome inhibitor PI31 subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_025986</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Plays an important role in control of proteasome function. Inhibits the hydrolysis of protein and peptide substrates by the 20S proteasome. Also inhibits the activation of the proteasome by the proteasome regulatory proteins PA700 and PA28.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the proteasome inhibitor PI31 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010001697">
        <property type="protein sequence ID" value="KAI7755116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D6A2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000502">
        <property type="term" value="C:proteasome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004866">
        <property type="term" value="F:endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070628">
        <property type="term" value="F:proteasome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1000.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045128">
        <property type="entry name" value="PI31-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013886">
        <property type="entry name" value="PI31_Prot_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021625">
        <property type="entry name" value="PI31_Prot_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13266">
        <property type="entry name" value="PROTEASOME INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13266:SF1">
        <property type="entry name" value="PROTEASOME INHIBITOR PI31 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08577">
        <property type="entry name" value="PI31_Prot_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11566">
        <property type="entry name" value="PI31_Prot_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-0647">Proteasome</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PI31 proteasome regulator N-terminal" evidence="13">
        <location>
            <begin position="15"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="PI31 proteasome regulator C-terminal" evidence="12">
        <location>
            <begin position="206"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="149"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="269"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="153"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="168"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF08577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF11566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7755116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="305" mass="32348" checksum="E4417B0DB182871D" modified="2024-05-29" version="1">MATEQSVLAVIRASRPNFRNSADKLVFAIHSIFLASGFNLNATGPPAFTDDALSASFSDEAGIEGWNEVEDNYAFVYSSPNGLKKVLVKCLVMNKQLFVDALVSGSSDPLHLEINIDDFVGETDDTKYSSQYKDLGKLVDSVNKGVLNKLNGSSTASTSNTASASSEQKMEDTDRDQPRGAPFGLEDPYVPYHPSGVGMPPVIPGIGGGDLYPQPPAGVYPTRGGFGDGGMLVGPHDPRFFGGGVGGRMGFPGGQPGVPPGARFDPFGPPDVPGFEPNRFTRNPRRPPGGTHPDLEHFNNGSDFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D771</accession>
    <name>A0AAD5D771_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_033544</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010003942">
        <property type="protein sequence ID" value="KAI7754267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D771"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051026">
        <property type="entry name" value="PI/PC_transfer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45657">
        <property type="entry name" value="CRAL-TRIO DOMAIN-CONTAINING PROTEIN YKL091C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45657:SF5">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL_PHOSPHATIDYLCHOLINE TRANSFER PROTEIN SFH6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7754267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="117" mass="13553" checksum="AA23B53E096B4AC4" modified="2024-05-29" version="1">MAFFMSLVAFFRSTMTRLTKKLHDVHVTFEETKVVSSVLERLGELENKVDTLKAKPSEMPYEKVALLNAAGFPIDALKAELIVTKKALHEALMRQEELLAYIDTQEEKMMRKKKFCW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D7N4</accession>
    <name>A0AAD5D7N4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_018813</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010002455">
        <property type="protein sequence ID" value="KAI7754814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D7N4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7754814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="66" mass="7457" checksum="E1B5AD97EB76DE7F" modified="2024-05-29" version="1" fragment="single">LLATKQESRRETTNKPAGTSIDDDKDDDDDEKMDSDLDSEQFTPAKRSFFSLPNAYLAVVSRLVDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D916</accession>
    <name>A0AAD5D916_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">S-formylglutathione hydrolase</fullName>
            <ecNumber evidence="2 7">3.1.2.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_026004</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Serine hydrolase involved in the detoxification of formaldehyde.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>S-formylglutathione + H2O = formate + glutathione + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14961"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:57688"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="EC" id="3.1.2.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the esterase D family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.12" evidence="2 7"/>
    <dbReference type="EMBL" id="JAMZMK010001697">
        <property type="protein sequence ID" value="KAI7755134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D916"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018738">
        <property type="term" value="F:S-formylglutathione hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046294">
        <property type="term" value="P:formaldehyde catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000002">
        <property type="entry name" value="S-formylglutathione hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000801">
        <property type="entry name" value="Esterase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014186">
        <property type="entry name" value="S-formylglutathione_hydrol"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02821">
        <property type="entry name" value="fghA_ester_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10061">
        <property type="entry name" value="S-FORMYLGLUTATHIONE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10061:SF0">
        <property type="entry name" value="S-FORMYLGLUTATHIONE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00756">
        <property type="entry name" value="Esterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0719">Serine esterase</keyword>
    <feature type="active site" description="Charge relay system" evidence="6">
        <location>
            <position position="153"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6">
        <location>
            <position position="230"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6">
        <location>
            <position position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR614186-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7755134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="286" mass="32216" checksum="233E0E4D9743EE13" modified="2024-05-29" version="1">MENTKPTEISSSKMFEGFNKRYKHYSPSLNCSMNFHIYFPPSSSPSQKFPVLYWLSGLTCTDENFIIKSGAQRVASTEGIALVVPDTSPRGLNVEGESDSYDFGVGAGFYLNATVEKWKNWQMYDYVVKELPKLLSDNFPQLDTSRASISGHSMGGHGALTIYLKNLDKYKSVSAFAPIVNPINCPWGQKAFTNYLGDDKSAWEEYDATCLISKFNDVAATILIDQGDDDKFLHENQLLPNKFDEACRNAKAPLLLRMQPGYDHSYYFVSSFIDDHIRHHAQALNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D9V3</accession>
    <name>A0AAD5D9V3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_029479</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000178">
        <property type="protein sequence ID" value="KAI7757126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D9V3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36038">
        <property type="entry name" value="OS06G0102750 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36038:SF3">
        <property type="entry name" value="OVATE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="195"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="204"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7757126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="338" mass="36718" checksum="B58EC3324C2511F0" modified="2024-05-29" version="1" fragment="single">EDILRDVWFPATIVEYMGKGLYLVEYNCSGSNDESALQRDSDSDDDVHDELRENATLNVTNPTGDTLASIDKQTCVTRSTKRSKATNPGSNDKLITPCKKLKSNGVPSKEVNNQSVDKTKRPLRGKKTPAKQDSSVDLKRPMITSARKKGKLSANSRGLKALVKAAGDLAPTTTPNTVKGKRVIKKSKQLIALSPQNVGEPEEPQSSVPLASKTTDDQGGGRGVVVGWFEAVLDDGFRGWTGWFDGDDDEDDGGRLDDDVNDGVAGMAVGSCRVADQVLKHVPNVEVVETISRMKELLRWVAAAKSEKGGKYIARKGSQFRNKVATKTIQDDDQLTQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DB56</accession>
    <name>A0AAD5DB56_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Importin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_023021</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010001055">
        <property type="protein sequence ID" value="KAI7755526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DB56"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040122">
        <property type="entry name" value="Importin_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057600">
        <property type="entry name" value="TORTIFOLIA1/SINE1-2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057672">
        <property type="entry name" value="TPR_IPO4/5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10527">
        <property type="entry name" value="IMPORTIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24714">
        <property type="entry name" value="TOR1L1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25780">
        <property type="entry name" value="TPR_IPO5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="9">
        <location>
            <begin position="23"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="8">
        <location>
            <begin position="378"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7755526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="1049" mass="116146" checksum="55803E801BBB9DE1" modified="2024-05-29" version="1">MSQSLELLLLQFLMPDNEARQKAEDQIKRLVRDLDVVPHLIDHIRSATTPNVRQLSAVLLRKKITGYWPMLTDEIRLLVKQSLIESIALECSPSVRRASANVVSIIAKHTVPGGEWPDLLPFIFQCSKSAQEDLRELGLLLFSSLIETIGDSFQPYFADLKLLLLNCLQDEISAVRVASLKAIGAFLESTHDESQVKKFCEYIPSILNYSRKCLSSGDDDNVIIAFEIFDELIESPAPLPGESIKAIAQFSLEVCSSSNTEISIRHQAIQIISWLAKYRSNSLMKHGLIIPILQVMCPLLTEATGSDFDDDLSADRAAAEVLDTISVKLSNHVFPTVFQFASEYSQNVDPKYREASIMVLGLISEGCSELMKEKLEHILHIVIDGLRDPEQVVRGSASFALGQFAEYLQPEIISHYEKILPRILRSLLQDASDDVKEKSYYALAAFSENMHEEIVPFLDTLLGRLFASLQNNPRILQETCLSAIGSVASAADKAFIPYAERVLELMKYFMVLKDEEDLRSRARATELVGIVAVIVGRQKMEPVLPPFIEAAVSGYGLECSELRKHTHGFFSNVTEVLEDGMIQYIPRVVPLAFSSCYLDDGPAVDISDSDEDEDVVGLGAFSSDDEAQDEPRLGDVSIRTDVLDEKAAATQALGLFALHTKSAYAPYLEESLMIMIKHSISFHEDVRLQAVTGLKHILTAAHVFLQGQNDGTSKMKQILDSVMAIYIKTMNQDDDKEVVAQTCMSVAQIITDFGYVAMESYMARLVESTLVLLHQMSACQQVEFESDIKVYDPGQNEVLMDAVTDLLPAFAKAMGSNFAPIFAILFNPLMEFAKGSSSPKDCTMVVACLAEVARNMRAPISGYISPLMPFVLKELASSSATNRRNAAFCVGELCKNGGDYSLKYFDDTLQCLYPLFEESEPDHAVRDNAAGAVARMIMAHQDYVPLNQVLPMLLKVLPIKEDHEESVPVYTCICNLVLSSNSQILQLVSDLVIVFVQVAISPLETREVKLQIGGAVSHLISLYGHQMKPILRSLPPAYARALAAIVPKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DD84</accession>
    <name>A0AAD5DD84_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_023881</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000338">
        <property type="protein sequence ID" value="KAI7756591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DD84"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042104093" evidence="1">
        <location>
            <begin position="17"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7756591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="87" mass="9635" checksum="5D62EBBC7B925386" modified="2024-05-29" version="1">MCVLRLLGALFADVYGSFPCYDFPVSVVMGFNCFDKASNNAEESGNHATTEEAAEDESKAARPLWCCSRLSLFTSFIFIAYIPSGFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G2B3</accession>
    <name>A0AAD5G2B3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RNase III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_004155</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011811">
        <property type="protein sequence ID" value="KAI7725950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G2B3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004525">
        <property type="term" value="F:ribonuclease III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030422">
        <property type="term" value="P:siRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00593">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1520.10:FF:000004">
        <property type="entry name" value="Endoribonuclease dicer-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1520.10">
        <property type="entry name" value="Ribonuclease III domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014720">
        <property type="entry name" value="dsRBD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000999">
        <property type="entry name" value="RNase_III_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036389">
        <property type="entry name" value="RNase_III_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14950">
        <property type="entry name" value="DICER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14950:SF53">
        <property type="entry name" value="RIBONUCLEASE 3-LIKE PROTEIN 3 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00035">
        <property type="entry name" value="dsrm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00636">
        <property type="entry name" value="Ribonuclease_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00535">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69065">
        <property type="entry name" value="RNase III domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00517">
        <property type="entry name" value="RNASE_3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50142">
        <property type="entry name" value="RNASE_3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RNase III" evidence="9">
        <location>
            <begin position="71"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7725950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="308" mass="35637" checksum="76BF471D38B4C178" modified="2024-05-29" version="1">MEVHLHLQKKMLCKVYGTEPKYSMASSSWWRSLFSPSNNHFTLLHCFKRLQDYANLKLVTSSQEKKALASLKEMEKIIGYKFNKKLLLQQALTHPSYQESESYERLEYVGDSILNFLISKQQFFMYPNLSPGSLTALRAANVDTEKLARVAVKYNFHKYLRHENPVLSKQIQVFIKAIEKYPFHSYGLIDAPKTLADVVESTIGAIYVDSNSSIDTTWEVAKILLEPMITPEMLQQNPVRKLNELCHKRKLKIRLRDKWLKQGIYEVFIDNQLIGKGVYKAKKEIALNRAAEDACNNILNNISESLDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G2N9</accession>
    <name>A0AAD5G2N9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_025461</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011936">
        <property type="protein sequence ID" value="KAI7725456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G2N9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11064">
        <property type="entry name" value="CYP86A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24296">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0349">Heme</keyword>
    <keyword evidence="5 6 7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 6 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="6">
        <location>
            <position position="454"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7725456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="510" mass="58496" checksum="60AD45A1F8183821" modified="2024-05-29" version="1">MINMEMTSFLLIFFIFLLIYYFFFFNTTSSKSITQFGFKNYPIFGTLPEFLLNRHRFLDWSTQVLSQCPTNTAVFGRPGKVNGVITANPANVEHMLKTNFENYPKGTRFIALLEDFLGRGIFNSDGETWRAQRKTASYEFNTRSLRNFVMETAAVELHTRFVPVLERAAALNKVVDLQDLLERYSFDNICKVAFNFDPGCLAGDGTSGSEFMKAFEEAATLSSARFMYILPGLYKIKKLLNFGSESKLRKSIATVHKFADDIIQSRITESTKEPNDDLLSRFMSISEYSPEFLRDIVISFILAGRDTTSSALTWFFWLLSSHPQVKQKILNELSTLRLSSNKSDHNSYVLEDLRQMHYLHAAISEAMRLYPPVPVDTKACLKPDILPDGTFIGAGWFVTYHTYAMGRMESVWGTDCNEFRPERWLDEENGDGIVVYRQENPFKYPVFHGGLRVCLGKEMAYTQMKLLAATIMEAFEVEVVVEGEEKKVPEHVLSLTMRMKDGLKVRVRKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G3I3</accession>
    <name>A0AAD5G3I3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_008595</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TIM16/PAM16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011726">
        <property type="protein sequence ID" value="KAI7726251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G3I3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005744">
        <property type="term" value="C:TIM23 mitochondrial import inner membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005341">
        <property type="entry name" value="Tim16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12388">
        <property type="entry name" value="MITOCHONDRIA ASSOCIATED GRANULOCYTE MACROPHAGE CSF SIGNALING MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12388:SF0">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03656">
        <property type="entry name" value="Pam16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0811">Translocation</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042241239" evidence="9">
        <location>
            <begin position="25"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7726251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="119" mass="13313" checksum="75A349E6EDCAEE5D" modified="2024-05-29" version="1">MAAKILANLIVMGSGILIRGMVQAYRQALQNASKTGVAQETLQNAVRRGSKAMTEQEARQILGVTEQSSWEEIAKASFMHCICCFQLYLFDHMESKVHRAKECLETVYQQKHQPGDPSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G4A4</accession>
    <name>A0AAD5G4A4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_031630</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011337">
        <property type="protein sequence ID" value="KAI7727677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G4A4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33564:SF29">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33564">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7727677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="142" mass="15790" checksum="B3E2BA479962D0D7" modified="2024-05-29" version="1">MSSILASNGVVLATAMAAGTAIFIAFCLQKPPTTTVSAAPFAVVHRPRPCINSDGKKKVKKKMKKVRFAEDVMEPRGNGDEFRRRLKSKRIHQNRNFLTMFENEGGQSKHLRNSKGLPANRMALYSGILRDRGVHRVAYSCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G4F7</accession>
    <name>A0AAD5G4F7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_005857</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011303">
        <property type="protein sequence ID" value="KAI7727872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G4F7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008143">
        <property type="term" value="F:poly(A) binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12306">
        <property type="entry name" value="RRM_II_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4B/4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236:SF65">
        <property type="entry name" value="NUCLEOTIDE-BINDING ALPHA-BETA PLAIT DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="96"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="18"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="29"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7727872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="224" mass="25757" checksum="F518DD461E36A7B9" modified="2024-05-29" version="1">MDLTTEQDQEHEVYGGEIPDEAEMDADFEVSRDETEDTQHNNSKDMEDMKKRLKEIEEEAGALREMQAKVEKEMGSVQEDPSASATQAEKEEADARSIYVGNVDYACTPEEVQQHFQSCGTVNRVTILTDKFGQPKGFAYVEFVEPESVQNSLLLNESELHGRQLKVAAKRTNVPGMKQFRGRRPNPYLGSYPRRPYMPGPSYYSPYGYGRFPRSRRPMWYMPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G600</accession>
    <name>A0AAD5G600_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidase C14 caspase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_003921</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C14B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010958">
        <property type="protein sequence ID" value="KAI7729627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G600"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12660">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029030">
        <property type="entry name" value="Caspase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050452">
        <property type="entry name" value="Metacaspase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011600">
        <property type="entry name" value="Pept_C14_caspase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48104:SF2">
        <property type="entry name" value="METACASPASE-1-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48104">
        <property type="entry name" value="METACASPASE-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00656">
        <property type="entry name" value="Peptidase_C14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52129">
        <property type="entry name" value="Caspase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase C14 caspase" evidence="2">
        <location>
            <begin position="92"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7729627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="258" mass="29434" checksum="4A6E7639916912B9" modified="2024-05-29" version="1">MSSSYRTVPMSSSYRRGMLSMNERGEFVSDDGDEDGETCLQPNKGGVKFFSDTEGLFQKMFYRRDVSDFKLLDSYKKSTLESIRNWETTKGKRALLCGVTYNKQKYKLKGANFDVINMRQLLISRFQFPSSSIHILAEIDTCPPPTKRNIQEALRWLVKDNQPGDSLVFYFSGHGSQILDFFDDELDGYDETICPVDFKTEGMITDNEINDTIVRPLKRGVKLHAIIDASHSGTILDLPHVYSTKMLSQSQESKCKVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G729</accession>
    <name>A0AAD5G729_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_010021</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010895">
        <property type="protein sequence ID" value="KAI7729881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G729"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7729881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="140" mass="16456" checksum="B982416FFD5BC0B4" modified="2024-05-29" version="1" fragment="single">RGDGDEEVETKSTSVLRHYPNGATETSVKNTITQKDKELIYGFGEVPIKIRMVSMLPYRLTYLRVVEKRKKKYQSYTHRWPDRRNGFEKMGLKNYEEKKDQAWGHSNCVDEKNSFGYMVYDLAHMVLTVLRIYNPAALEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G771</accession>
    <name>A0AAD5G771_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_006826</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010579">
        <property type="protein sequence ID" value="KAI7730977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G771"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015369">
        <property type="term" value="F:calcium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004713">
        <property type="entry name" value="CaH_exchang"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31503:SF83">
        <property type="entry name" value="GUANYLATE CYCLASE ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31503">
        <property type="entry name" value="VACUOLAR CALCIUM ION TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0050">Antiport</keyword>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="110"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="282"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="319"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="360"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="393"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="163"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="203"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7730977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="474" mass="52607" checksum="B530ED63A07C6E6A" modified="2024-05-29" version="1">MGVSASDDTGAYFSAAMGMNVLAGSAVLSLTLIWPSVIVFGSYDLADDDDETGLSQLFEEEPSFIKKLTAYGLTTDTETSYTARLMLVSMIPFLILQLPQIINSASVTRVIVLLTLIISLGFFIAYAVYQIFQPWIQNRRFDYVRQKFVKNKLLKLLSTNGKANVQLIKELHKELDKNHDGKVTNAELKTLLLGIQLQADGKVSEDLVERVMDQLDISGDESIQEDEFVRILTKWLKDARKSLSKNDYNPLSFFTKPQADADEEEQAALIPKKENLNDQSSIWDYLEALALVLLGTAITALVALPLIMNVSGFATEANVPSFLIPYIIIPCAINIPRLLSTINSASQKTQRAASLTLSQIYSGVFMSNMSSLTTFLLTVYIRDLPWDVSAEVLVVLIICGVMGVFTSTRTIYPLWTGTFLKSVIKFVSYLVISCNHVIDVAYSNNLDTIRALNPISCPKHQKTRALNTKMVQGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G7L1</accession>
    <name>A0AAD5G7L1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Diphosphomevalonate decarboxylase</fullName>
            <ecNumber evidence="10">4.1.1.33</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_023095</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(R)-5-diphosphomevalonate + ATP = isopentenyl diphosphate + ADP + phosphate + CO2</text>
            <dbReference type="Rhea" id="RHEA:23732"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57557"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.1.1.33"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the diphosphomevalonate decarboxylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.33" evidence="10"/>
    <dbReference type="EMBL" id="JAMZMK010010821">
        <property type="protein sequence ID" value="KAI7730258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G7L1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004163">
        <property type="term" value="F:diphosphomevalonate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019287">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016126">
        <property type="term" value="P:sterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="entry name" value="GHMP kinase, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005935">
        <property type="entry name" value="Mev_decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053859">
        <property type="entry name" value="MVD-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041431">
        <property type="entry name" value="Mvd1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10977">
        <property type="entry name" value="DIPHOSPHOMEVALONATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10977:SF3">
        <property type="entry name" value="DIPHOSPHOMEVALONATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18376">
        <property type="entry name" value="MDD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22700">
        <property type="entry name" value="MVD-like_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015950">
        <property type="entry name" value="Mev_P_decrbx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="GHMP Kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6 10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9 10" id="KW-0456">Lyase</keyword>
    <keyword evidence="2 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="5" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="7" id="KW-1207">Sterol metabolism</keyword>
    <feature type="domain" description="Diphosphomevalonate decarboxylase-like N-terminal" evidence="12">
        <location>
            <begin position="17"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="Diphosphomevalonate decarboxylase-like N-terminal" evidence="12">
        <location>
            <begin position="68"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="Mvd1 C-terminal" evidence="11">
        <location>
            <begin position="111"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR015950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF18376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF22700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7730258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="308" mass="33858" checksum="CA9BB7E60E062ACB" modified="2024-05-29" version="1">MGGEQEKWILMVTARTPTNIAVIKYWGKRDETLILPINDSISVTLDPSHLCTTTTVSVSPTFDQDRMQGSGSACRSLYGGFVKWDMGKEDNGSDSLAIQLADEKHWEELVIIIAVVSARQKETSSTSGMRDTVETSALIQHRAKEVVPKRVVQMEDAIQKRDFPSFARLACTDSNQIISCVEKWNRAEGTPQVAYTFDAGPNAVMIARNRQTAALLLQRLLYNFPPQSDTDLDSYIIGDKSILKDAGIKDMKDVEALTPPPEVKDNIPPAQKSKGDVSYFICTRPGGGPVVLSDETQALLDPLTGLPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G9B4</accession>
    <name>A0AAD5G9B4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FAD-binding PCMH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_014277</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010300">
        <property type="protein sequence ID" value="KAI7732146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G9B4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.462.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32448">
        <property type="entry name" value="OS08G0158400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="1">
        <location>
            <begin position="5"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7732146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="358" mass="40690" checksum="3DC663C4D63B11CB" modified="2024-05-29" version="1" fragment="single">FNNPSTPKPSVIVTPTDETHIQAALLCAKKHGYEMRIRGGGHDYEGLSYTADVPFVMVDLVNMRSIDINVENRTAWVQGGALLGELYYTISQKTDTLYFPAGVCPSVGLRLVPVPERVTIFMVNITIEQGATDVFHKYQYVLPKFDRELQMRVQINSENIDNSTKKTIRILFHGIYQGNTDTLLPLLNQSFPELNVTREVCQEVRMVQSTLQFGGFTTSTPVEVLANRSAIPKTNFKGKSDYVRTPIPKRGLRKIWRKMFENDNSEMLQLYTFGGKMEEYSDTATPYPHRAGVLYQIFKLVNLIDQPSDMTTISLRRIAWLRSFDKTLKPYVSSNPREAYLNYIDLDLGVGKGSYEEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GBJ8</accession>
    <name>A0AAD5GBJ8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Expansin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_019963</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Causes loosening and extension of plant cell walls by disrupting non-covalent bonding between cellulose microfibrils and matrix glucans. No enzymatic activity has been found.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Secreted</location>
            <location evidence="7">Cell wall</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Membrane</location>
            <topology evidence="7">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the expansin family. Expansin A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009485">
        <property type="protein sequence ID" value="KAI7735444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009664">
        <property type="term" value="P:plant-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22274">
        <property type="entry name" value="DPBB_EXPA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.10:FF:000001">
        <property type="entry name" value="Expansin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.760:FF:000001">
        <property type="entry name" value="Expansin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.760">
        <property type="entry name" value="Expansin, cellulose-binding-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="entry name" value="RlpA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007118">
        <property type="entry name" value="Expan_Lol_pI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002963">
        <property type="entry name" value="Expansin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007112">
        <property type="entry name" value="Expansin/allergen_DPBB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007117">
        <property type="entry name" value="Expansin_CBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036749">
        <property type="entry name" value="Expansin_CBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009009">
        <property type="entry name" value="RlpA-like_DPBB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31867">
        <property type="entry name" value="EXPANSIN-A15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03330">
        <property type="entry name" value="DPBB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01357">
        <property type="entry name" value="Expansin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01226">
        <property type="entry name" value="EXPANSIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01225">
        <property type="entry name" value="EXPANSNFAMLY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00837">
        <property type="entry name" value="DPBB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="Barwin-like endoglucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49590">
        <property type="entry name" value="PHL pollen allergen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50843">
        <property type="entry name" value="EXPANSIN_CBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50842">
        <property type="entry name" value="EXPANSIN_EG45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0134">Cell wall</keyword>
    <keyword evidence="6 7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041779604" description="Expansin" evidence="7">
        <location>
            <begin position="21"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="Expansin-like EG45" evidence="8">
        <location>
            <begin position="49"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="Expansin-like CBD" evidence="9">
        <location>
            <begin position="174"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7735444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="258" mass="28213" checksum="C30F5BD7B5D822C3" modified="2024-05-29" version="1">MGILVGILLIIISLFLVADARIPGNYAGGSWESAHATFYGGSDASGTMGGACGYGNLYSQGYGVNTAALSTALFNKGFSCGACFEIKCAQDPRWCHPGSPSIFITATNFCPPNYALPNXXXXGWCNPPRTHFDLAMPMFLKIAEYRAGIVPVSYRRVPCRKKGGIRFTINGFRYFNLVLISNVAGAGDIQKVWVKGTKTSWMSMSRNWGQNWQSNAVLVGQSLSFRVTASDRRTSTSWNIVPAHWQFGQTFTGKNFRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GCY4</accession>
    <name>A0AAD5GCY4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">ABC transporter D family member 2, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_030761</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily. ABCD family. Peroxisomal fatty acyl CoA transporter (TC 3.A.1.203) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009463">
        <property type="protein sequence ID" value="KAI7735598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GCY4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03223">
        <property type="entry name" value="ABCD_peroxisomal_ALDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050835">
        <property type="entry name" value="ABC_transporter_sub-D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11384">
        <property type="entry name" value="ATP-BINDING CASSETTE, SUB-FAMILY D MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11384:SF59">
        <property type="entry name" value="LYSOSOMAL COBALAMIN TRANSPORTER ABCD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06472">
        <property type="entry name" value="ABC_membrane_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="136"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="11">
        <location>
            <begin position="136"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="341"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="50"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="51"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7735598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="613" mass="69284" checksum="86B943D689B8F535" modified="2024-05-29" version="1">MTQSNTLVFCNVNVSAIRYNNHHNTTNNNNNNNRISFTIPFKSHRMKRRNNFTPLSSSPTTPADPSVTPQQQPVKTDENVPRKGPDLPTLFRRFYKVAAPYWFSDDKVFVIRDYARETLALRWRSWMTNFYMERNILFGIYPPLFVVLLVYSLGGTAISIFLGKGLVNLNFMQEKMEADFRYGLVRIRENAESIAFYGGEENEIKLLLQRFRNAFENLTQLLISSRNLEFFTNGYRYLIQILPAAVVAPMYFSGKIEFGVINQSVSAFNHILGDFSLIVYQFQAISAFSAVIDRLGEFDDILDTTSSDDPVEALDEIQLTYINVKDATFQPKNSLDAQKLLEIENLTVLTPTTKALLINDLSLTVCNNEHLLITGPSGSGKTSLLRAIAGLWRTGRGKITFCAKYTPEMASNGVAMVNEINNVDKKSKYRDFKGVFFLPQRPYMVLGTLRQQLLYPTWPDVSDSTSHNSKSVGLFPFLEDKSGPEVGSAQPLQPDTDELVKALEDVHLGYLLSRFSGLDTTNEWSSVLSLGEQQRLAFARLLLSRPDLVLLDESTSALDEANEASLYNKINAAGITYISIGHRTSLRKFHKKALRISPMDLDSNQLNWCIEPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GDH2</accession>
    <name>A0AAD5GDH2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Condensin-2 complex subunit D3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_016094</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009276">
        <property type="protein sequence ID" value="KAI7736421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GDH2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000779">
        <property type="term" value="C:condensed chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000796">
        <property type="term" value="C:condensin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010032">
        <property type="term" value="P:meiotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007076">
        <property type="term" value="P:mitotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026971">
        <property type="entry name" value="CND1/NCAPD3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14222">
        <property type="entry name" value="CONDENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14222:SF1">
        <property type="entry name" value="CONDENSIN-2 COMPLEX SUBUNIT D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0226">DNA condensation</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="720"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1149"/>
            <end position="1179"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="1063"/>
            <end position="1090"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="722"/>
            <end position="741"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7736421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1195" mass="131906" checksum="60B0104C0ABB9A78" modified="2024-05-29" version="1">MEKHINKIVTELYHHHTNPNPQQPITQSTLQSLQTLLDYTLNKDDPIELENLSDELSSHNLSLSTSLLTPLTAAMDSGGPHHSLLASKCSQVGLVNSHVGRKKKGGRLKGRGKNVIFSGYEDESIESDVVREFDVRVLFPVLERLHLVLGLIHLDRFPDCLKGLVQTMGAIPAIALEACSGSGLGSFNRLCDLCGEILSELLKGEEHGDKGVTAGEVMKALSCSIILSKSQARTFGLGYVVNKMGVMARESDEIRKAMVNFPKYLIGKAPERAELRALAVESILEVVGCLEYEDQIGFSEYVVNMTKGKPQFRLLAVDLILNLIMSLDNPLGHESSWGVKCLRALLERCSDSTAGIRARAITNLAQLVGFLSGNNENKMELMKIMGFGEDGGLNDLLRKRCVDEKAAVRKSALVLISKLTALQGEGGGFDDVLLKIMGVACSDQLVSIRKAAVSALSETFRKFDDENVTKEWLHSVPRLITDNETSIQEECENLFLELVLDRISRAGYNVDDLSLSDGVLSLLKEICNGEVSPWVKKICTNLGKKKKLKPKVAVCLQNIIKKSESMWLTDSKPLEKWLAPPGAWFLLSEVSGFLLKAVDWEFLYHHWQLLDKHESSNAPLEEEDIESNSVDWAGDRTISNVSVELPAEAAADLAHNLLKRIEDFSMHSMEVDAHVKALRTLCKRKAKDPQEADSLVIKWVHQLLSKASKTIQAYMTKDSENNKNNSLFTPQSGTRKGNRSSTTMSKLLAQATTAVYTIGSLVLVCPSADLKTITPILHNIITSGVSDPKHKKSPETAPSLYIQAWVAMGKICLADGKLAKQYIPLFVQCIRLLQEMEKSDIASLRNNIVRDYVKWRGVVFLRFLLSLVDESEKIRQLADFLFGNILKVKAPLLAYNSFVEAIFVLNDCNAHTGQSNPLSSQKESRLFSIRGNDEKSKSQRMHIYVSLLKQMAPEHLLATFAKVCAEILASASDGMLKIDDVTSQSVLQDAFQVLSCKEIKIPTNKGSSTESSEMEEDVAENAIKGRVITQVVKKSLIQNTIPIFIELKRLLESNNSPLTGSLMECLRILLKDYKNEIEEMLVADKQLQKELIYDMQKYEAQKARSTAAEAVDKGINVNMHTCSKVSSAVAGALAEATAKSVLRDVNQGAGTPSLSAMNVPKLKSSTGTAAKHKGRSSAVLESLRRRQSFDLDDDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GDM6</accession>
    <name>A0AAD5GDM6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">glucan endo-1,3-beta-D-glucosidase</fullName>
            <ecNumber evidence="4">3.2.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">M8C21_002014</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">AA19_3_7</strain>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of (1-&gt;3)-beta-D-glucosidic linkages in (1-&gt;3)-beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.39"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <topology evidence="2">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the glycosyl hydrolase 17 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.39" evidence="4"/>
    <dbReference type="EMBL" id="JAMZMK010009299">
        <property type="protein sequence ID" value="KAI7736333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GDM6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042973">
        <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000008">
        <property type="entry name" value="Glucan endo-1,3-beta-glucosidase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1040:FF:000002">
        <property type="entry name" value="Glucan endo-1,3-beta-glucosidase 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000490">
        <property type="entry name" value="Glyco_hydro_17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044965">
        <property type="entry name" value="Glyco_hydro_17_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012946">
        <property type="entry name" value="X8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32227">
        <property type="entry name" value="GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00332">
        <property type="entry name" value="Glyco_hydro_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07983">
        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00768">
        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0611">Plant defense</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="domain" description="X8" evidence="16">
        <location>
            <begin position="354"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="17">
        <location>
            <position position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAI7736333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="470" mass="52520" checksum="89286F7CEEDB6710" modified="2024-05-29" version="1" fragment="single">TVLRPEADRVQSIGVNWGTSASHPLPPSKVVELLKLNKITKVKLFDSNPQVLEALSNSVIDVTVGVPNSMLRSLNTSVKAAESWVHDNLTRYLTTGRTRIEYITIGDEPFLQSYGSRFYPFVVGAAINIEAALMRAKLSKKVKVVIPCSFDAFGSESGQPSKGHFRPDINKTMIQVLNFLTKTRSPFFVNISPFLSYYQNKNISLDFALFKEETARPHNDTHRSYKNSFELSYDTLITALTVAGFPNIDIVISQIGWPTDGAPNATVSNAEIFIKGILDHFRNRKGTPLRPRVMPVEIYIHALLDEDQRNITAGNFERHWGVFTFDGQAKYQVDFSQSTKSLVNAQSVEYFASRWCVVNNNLDMSNATAHAMEACTNADCTALSPGSSCFNLSWPGNVSYAFNSYYQQHDQRADSCDFGGLGMITTVDPSVGNCRFNVELKASFSVSLRWSKFDYPLILIVLAVWLLDRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GEB4</accession>
    <name>A0AAD5GEB4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_012972</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the alkB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008835">
        <property type="protein sequence ID" value="KAI7738309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GEB4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032451">
        <property type="term" value="F:demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006402">
        <property type="term" value="P:mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.590">
        <property type="entry name" value="Alpha-ketoglutarate-dependent dioxygenase AlkB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027450">
        <property type="entry name" value="AlkB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037151">
        <property type="entry name" value="AlkB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044842">
        <property type="entry name" value="ALKBH9B/ALKBH10B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31447">
        <property type="entry name" value="HYDROXYPROLINE-RICH GLYCOPROTEIN FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31447:SF1">
        <property type="entry name" value="OS06G0138200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13532">
        <property type="entry name" value="2OG-FeII_Oxy_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="3">
        <location>
            <begin position="290"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="98"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="440"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="461"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7738309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="471" mass="52858" checksum="E40E01762DA5E537" modified="2024-05-29" version="1">MNGIHSVVEVPNPEEMSTKKDWLSIIKGFEREEVDELLSQTFCGHCERLLHDRVLSIANRKRAKKLSISSVEATQLDDIPDRCLESCIQESLNKSDLVESPGNTHFGGQKRYVDKFSPTSMDSKRVTSSSSSKSGTRFDNGVHDTSSMGDELQSEEQKERLRFVNVGRKTNFTHFEKVNGKNINVVQGLELHTRVFSDEEQKKIVESVYEFQRMGQKGRLRERTYSEPSKWMRGKGRITIQFGCCYNYAVDKNGNPPGIIRDEEVDPLPPIFIDMIKRLVRWHVLPPTCVPNSCIVNIYEEGDCIPPHIDHHDFVRPFCTISFLTQCNILFGSNLKIIGPGEFAGPFSIPLPVGSVLVLKGNGADVAKHCVPAVPAKRISITFRKMDESKIPYSYSPDPKLQGVWPLTITPHKSPSQQKEISTKETSHVFVPRAVLKQSNPSLFNGEDDFPPLGAAVGSRHSGHKKNVSKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GEJ7</accession>
    <name>A0AAD5GEJ7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_011588</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SNF2/RAD54 helicase family. RAD16 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009004">
        <property type="protein sequence ID" value="KAI7737516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GEJ7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008094">
        <property type="term" value="F:ATP-dependent activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18008">
        <property type="entry name" value="DEXDc_SHPRH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050628">
        <property type="entry name" value="SNF2_RAD54_helicase_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626:SF12">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="12">
        <location>
            <begin position="140"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="11">
        <location>
            <begin position="493"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="27"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="297"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="41"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="306"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="329"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7737516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="750" mass="84724" checksum="0288CC11260917CA" modified="2024-05-29" version="1">MYLMCFYGVHICIDNDEEVDVSDSEVDERKRRKTSSWSGRRKVEQRDERSNEGLAVEDDLDKQIDLSVKKKTKKKVKKTKPILMWEVWEEENEKWLDQHMTADVDLDNLNEIVAETVDPSPDLIMPLLRYQKEWLAWGLKQEESASRGGILADEMGMGKTVQAIALVLAKRALCRELGDPAELSTGSSTGLPCVKTTLVICPLIAVMQWVNEIDRFTTKGSNKVLVYHGPNRGKSLIEFSEYDFIITTYSIVEAEYRRNVMPPKDRCQWCGKLFYAKKLKIHLKYFCGPDAVKTAKQSKQSKKKGKLGDKSSLSKEDLSDEEDDDDRKSSKKNTKGKRKKENIETGGFPDISSGSGCKSSVKSFLHSVKWDRIILDEAHYIKDRRCNTTRAVFALESSYKWALSGTPLQNRVGELYSLVRFLQITPYSYYMCKDCDCKVLDYSSNSSCPGCPHKSVRHFCWWNKAVDHPYLVVYSKSAVARRSETKSTDEQFCGLCHDVAEDPVVTSCEHLFCKSCLIEFSASYGQPACPSCSKPLTVDFGANNHDQEVQKPKSSFKGFKSTSIINRIRLENFQTSTKIDALKEEIRFMVERDGSAKGIVFSQFTSFLDLIHYSLQKCGVKCVQLDGSMTMAAREAAITRFTDDRECKVFLMSLKAGGVALNLTVASHKLPVAAVLELPTAIAAVLRSSSPPPPTTVYRWVVRFVIENTIEERILNLQEKKKLVFEGTVGGCNEALGRLTEADMRFLFAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GIF3</accession>
    <name>A0AAD5GIF3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_009282</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007630">
        <property type="protein sequence ID" value="KAI7743920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GIF3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7743920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="103" mass="11670" checksum="7975762E02675A4E" modified="2024-05-29" version="1" fragment="single">LLSCRQTDWWSIQSPPSTKSEEIGVVLRSLQQTKTPARSEWSLGIITKVSILTPPKLSLANITFLACQDYIRCQKLPFQTYRRLGEILSTRCPEYTAILSALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GIP6</accession>
    <name>A0AAD5GIP6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_023962</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008140">
        <property type="protein sequence ID" value="KAI7741731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GIP6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7741731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="146" mass="16456" checksum="F2D49D41F7FBFEE9" modified="2024-05-29" version="1">MLGMSAMQLLEISKQLLSGEDKKHPAEFDVFEEKKFAFKIRVGQFNIDKHLDGYGIRKLTNDSSIVASLEERFPHIQDSVGDNTTQESSARVSFISDVQEVKCSLDKVYELDDDAAASTTKILKSDEKCYSNKSSETPFLIPKQEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GK56</accession>
    <name>A0AAD5GK56_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_024697</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007094">
        <property type="protein sequence ID" value="KAI7745857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GK56"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7745857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="59" mass="6913" checksum="963CE8FC5C46C5D0" modified="2024-05-29" version="1">MNILDVMVEEELDKSRWKMPDEETEDQDDLWGFEDLEFEFYESSTSSPSLQDSVSTKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GKB6</accession>
    <name>A0AAD5GKB6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">1-phosphatidylinositol 4-kinase</fullName>
            <ecNumber evidence="2">2.7.1.67</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_020807</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PI3/PI4-kinase family. Type II PI4K subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.67" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010007572">
        <property type="protein sequence ID" value="KAI7744189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GKB6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004430">
        <property type="term" value="F:1-phosphatidylinositol 4-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044571">
        <property type="entry name" value="P4KG1-8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45800:SF11">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3-KINASE-RELATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45800">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4-KINASE GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7744189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="210" mass="24137" checksum="E12367D933EE3ED3" modified="2024-05-29" version="1" fragment="single">VVKKMAVGLRDVSDTRSRTHNEGRILSYFGGISNSRNTSPIRRVLGFRNTSQCRPKEIKTKLSGLDENNKPLTQSWRILPSKHYNQHTQLIMSFKVIEASDIYVDNKPLPTELWDCMFVQINNIFHFQSVLVFEEPFLHTTIQKGLKPSVRVGETWFREVTTFLLDHDQSAEVPPTTLVKTNHSIFNVNHEVGEIMLQYTIHACKVASLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GL71</accession>
    <name>A0AAD5GL71_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Caffeoyl-CoA O-methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_005262</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-dependent O-methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007392">
        <property type="protein sequence ID" value="KAI7745014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GL71"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009809">
        <property type="term" value="P:lignin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050362">
        <property type="entry name" value="Cation-dep_OMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002935">
        <property type="entry name" value="SAM_O-MeTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10509:SF64">
        <property type="entry name" value="CAFFEOYL-COA O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10509">
        <property type="entry name" value="O-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01596">
        <property type="entry name" value="Methyltransf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51682">
        <property type="entry name" value="SAM_OMT_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0438">Lignin biosynthesis</keyword>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7745014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="115" mass="12921" checksum="011DF70EBB07A591" modified="2024-05-29" version="1" fragment="single">MAATGETQPAKHQEVGHKSLLQSDALYQYILETSVYPREPQPMKELREVTAKHPWNLMTTSADEGQFLNLLLKLINAKNTMEIGVYTGYSLLSTALALPEDGKILALDINRENYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GLV9</accession>
    <name>A0AAD5GLV9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">M8C21_022267</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">AA19_3_7</strain>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HD-ZIP homeobox family. Class IV subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007379">
        <property type="protein sequence ID" value="KAI7745031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GLV9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08875">
        <property type="entry name" value="START_ArGLABRA2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000229">
        <property type="entry name" value="Homeobox-leucine zipper protein HDG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042160">
        <property type="entry name" value="HD-Zip_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057993">
        <property type="entry name" value="HD-Zip_IV_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002913">
        <property type="entry name" value="START_lipid-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45654:SF69">
        <property type="entry name" value="HOMEOBOX-LEUCINE ZIPPER PROTEIN ANTHOCYANINLESS 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45654">
        <property type="entry name" value="HOMEOBOX-LEUCINE ZIPPER PROTEIN MERISTEM L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25797">
        <property type="entry name" value="PDF2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01852">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00234">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50848">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 9 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 9 10" id="KW-0371">Homeobox</keyword>
    <keyword evidence="8 9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="13">
        <location>
            <begin position="115"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="START" evidence="14">
        <location>
            <begin position="321"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="9">
        <location>
            <begin position="117"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="76"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="169"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAI7745031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="814" mass="88477" checksum="831066FE9D64390D" modified="2024-05-29" version="1">MSFGGCFGGSGDSSMLMADTSFNNNVPFHRAPYSQQQLITSTIPQPIYNSQSLSLALKPKMEGVGDHVGLIGENFEGNIGRVRGDGSDSKSGGSDNVEGASGDEHDIPVGPSSNTRRRQKYHRHTAYQIQELEASFKDNPHPDEKERLALGKKLNLSNKQVKFWFQNRRTQMKTQMERHENAILKQENDKLRIENVAMKEAIRTPVCNNCGGQAMVGDISIDEHHIRIENARLRDELNRVSILANKFLGRPLSSFGGSLPQGMGSSYLELGIGRNGYGLNPMDVGLPMGLDFTNGFSNGLPLMSPSRPGIMAANGGGGMDLSYEKSMFLDLAMSAMDELMKLGQPNNLLWIGNIEGGGEVLDLNEYLRNFPLCLGMKPHDYVSDASRASGVVMINSLGLVDSLLNVNRWRDTFLGLIGSCSIIEVISDGNGDSRNGVVQLMEAEIQLVSPQVPARQLRFIRYCRQHGEGLWAVVDVSVDAAGREGFISRKLPSGCIVRNMPNGFSTVTWIEHTEYNESVIPHQYRPLLRSGLSFGAQKWISTLQRHFECVATIMSPDRTIEDGPVLSLGCKRSLTSLAQRMTANFCAGVCATGGHKWERVSDTPEPARILIRKSLNNLGDPSGGVLSASMSVWMPMQHQRLFALMLNEDMRSKWDVLSPGSAMQNMISLSKSQDNIGNLNTISLRANATGPNANQNSVLVLQDSATDVTGSLMVYAAVDVEVVTIVMNGGDSSCVALLPSGFAIVPDCVAGSGAKAEGEAGSLLTVGFQILVNDLPSSNIKKESVNTVINLITRTIQGIQDVVRADQQGRQITN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GM48</accession>
    <name>A0AAD5GM48_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_024467</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007303">
        <property type="protein sequence ID" value="KAI7745286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GM48"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016760">
        <property type="term" value="F:cellulose synthase (UDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030244">
        <property type="term" value="P:cellulose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005150">
        <property type="entry name" value="Cellulose_synth"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13301">
        <property type="entry name" value="X-BOX TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03552">
        <property type="entry name" value="Cellulose_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="binding site" evidence="8">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR605150-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7745286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="117" mass="13241" checksum="D7FF50794EAE2BEC" modified="2024-05-29" version="1" fragment="single">MVSSCIPWHHVSLFFVPYQEPPLVTTNTVLSNLAVDYPFDKVSCYVSDDGAVMLSFESLSETSEFARKWVLFYKKYNIEPRAPELSFDPLDFSTKKGESSTWMIGSCLGNLLTLGDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GM53</accession>
    <name>A0AAD5GM53_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_017396</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the caleosin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006617">
        <property type="protein sequence ID" value="KAI7748000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GM53"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007736">
        <property type="entry name" value="Caleosin-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31495:SF50">
        <property type="entry name" value="PEROXYGENASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31495">
        <property type="entry name" value="PEROXYGENASE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05042">
        <property type="entry name" value="Caleosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="90"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7748000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="238" mass="27103" checksum="D1C5F661414AD58B" modified="2024-05-29" version="1">MAMADGNALTPEADCQPLTRERKVRTDLETHLPKPYLARGLVAPDTEHPNGTLGHRHYDMTVLQQHVSFFDHNNDGIIYPWDTYNGMRELGFNMLISLLAAMFINIGMSYPTLPGWIPSPFFPIYIYNIHRAKHGSDSATYDTEGRYMPVNFENIFSKYARTVPDKLTLGEIWGMTEGNRIAFDPFGWTAGKMEWGILYALAKDEEGMLSKEAVRRCFDGSLFDYCAKMNRAAHGKNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GMP8</accession>
    <name>A0AAD5GMP8_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_006420</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007296">
        <property type="protein sequence ID" value="KAI7745318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GMP8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038974">
        <property type="entry name" value="CIF1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35290:SF2">
        <property type="entry name" value="PROTEIN CASPARIAN STRIP INTEGRITY FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35290">
        <property type="entry name" value="PROTEIN CASPARIAN STRIP INTEGRITY FACTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042295970" evidence="2">
        <location>
            <begin position="24"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7745318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="90" mass="10301" checksum="CC5F1AAD28993781" modified="2024-05-29" version="1">MMVFKTCLLCYLIVTSLVSSSFSARCESSIPMMKVDASDQNKESLYSEDDQSLKRDDIIHERLLRANTRDYGRSDPAPTFHKPPFKLIPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GR41</accession>
    <name>A0AAD5GR41_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_022864</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006444">
        <property type="protein sequence ID" value="KAI7748918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GR41"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.110.10">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037176">
        <property type="entry name" value="Osmotin/thaumatin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001938">
        <property type="entry name" value="Thaumatin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31048">
        <property type="entry name" value="OS03G0233200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00314">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002703">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00205">
        <property type="entry name" value="THN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49870">
        <property type="entry name" value="Osmotin, thaumatin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51367">
        <property type="entry name" value="THAUMATIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="75"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="80"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="88"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="104"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="114"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR002703-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7748918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="169" mass="17617" checksum="DDC285130459A024" modified="2024-05-29" version="1">MVWPGILSSAGSTPLDSTGFELSPGELRSFQASPGWSAEFTIGSGSNPQDFYDVSLVDGYNLPIAVDASGGTGVCGTTGCVTDLNRMCPAELRAGDGQACKSACEAFGNPEYCCSGSFGSPDTCHPSVYSQVFKSACPRSYSYAYDDATSTFTCTGADYTITFCPTSTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GRH7</accession>
    <name>A0AAD5GRH7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_011785</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006272">
        <property type="protein sequence ID" value="KAI7749831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GRH7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7749831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="62" mass="6949" checksum="36CCBA35FDFF0292" modified="2024-05-29" version="1">MGHASGFYTYTISKGDSSLLLCFTRVVIRGFHHWFLSSTYHPYPLSTISSSPFSPSLHLQPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GRV1</accession>
    <name>A0AAD5GRV1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">THH1/TOM1/TOM3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_032064</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the plant tobamovirus multiplication TOM1 protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006011">
        <property type="protein sequence ID" value="KAI7751044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GRV1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009705">
        <property type="term" value="C:plant-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040226">
        <property type="entry name" value="THH1/TOM1/TOM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009457">
        <property type="entry name" value="THH1/TOM1/TOM3_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31142:SF1">
        <property type="entry name" value="TOBAMOVIRUS MULTIPLICATION PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31142">
        <property type="entry name" value="TOBAMOVIRUS MULTIPLICATION PROTEIN 1-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06454">
        <property type="entry name" value="THH1_TOM1-3_dom"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="120"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="158"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="198"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="THH1/TOM1/TOM3" evidence="8">
        <location>
            <begin position="19"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="THH1/TOM1/TOM3" evidence="8">
        <location>
            <begin position="76"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF06454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7751044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="228" mass="26199" checksum="54F7CC37E88D6CA0" modified="2024-05-29" version="1">MIRTLLSIPSMKNSLWSSSSWSEEINKSQDGIFFTLCGFYALVSAIALIQLIRIEIRLPEYGWTIQKVFHLMNFIARNLPTDTLKATYTRVNVGVYVIQACVWIYRWLDDRSMVHFVGKLFLAVVSFMAALGFLLYGVRLFFMLRRYPVESEGKRRKLHEVGSVATICFTFFVVRYFVDVLSAFDSEVSLDVLGRPVLNLIFYMLAEVLPSALVLFILRKLPPKRASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GS18</accession>
    <name>A0AAD5GS18_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Mitochondrial adenine nucleotide transporter BTL1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_028947</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006150">
        <property type="protein sequence ID" value="KAI7750476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GS18"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24089">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 6 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="238"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="281"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="6">
        <location>
            <begin position="71"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="6">
        <location>
            <begin position="182"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="6">
        <location>
            <begin position="280"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7750476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="371" mass="40496" checksum="D7E0E5DF07EC11C0" modified="2024-05-29" version="1">MTPKSTSHPQQRKGFCAMDDVYGSIVESVPLHKNLDLVFLGQNNSTTKDDFFTMDFQLPDFRPPIAKFVKTREVGEFLSGALAGAMTKAVLAPLETIRTRMVVGVGSKTISGSFIEVMEKQGWQGLWAGNAINMIRIIPTQAIELGTFECVKRVMTTAKEKWSQEECPSVQLGPVKLSFSLSWLSPVAVGGAAAGIAGTLACHPLEVIKDRLTVSPDVYPNLSIAVQKMFKSGGIGSFYSGLSPTLMGMLPYSTCYYFMYDTIKKSYCTAQKKKSLSRPEMLLIGALSGLTASTISFPLEVARKRLMVGALQGKCPPHMAAALAEVVREEGLIGLYRGWGASCLKVMPSSGITWMFYEAWKDILIGDKDKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GST3</accession>
    <name>A0AAD5GST3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">M8C21_013050</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">AA19_3_7</strain>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006093">
        <property type="protein sequence ID" value="KAI7750748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GST3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015421">
        <property type="term" value="F:ABC-type oligopeptide transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010329">
        <property type="term" value="F:auxin efflux transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010328">
        <property type="term" value="F:auxin influx transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090374">
        <property type="term" value="P:oligopeptide export from mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18578">
        <property type="entry name" value="ABC_6TM_Pgp_ABCB1_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03249">
        <property type="entry name" value="ABC_MTABC3_MDL1_MDL2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000009">
        <property type="entry name" value="ABC transporter B family member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000066">
        <property type="entry name" value="ABC transporter B family member 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394:SF16">
        <property type="entry name" value="ABC TRANSPORTER B FAMILY MEMBER 4-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394">
        <property type="entry name" value="ATP-DEPENDENT PERMEASE MDL1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="65"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="491"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="535"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="624"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="14">
        <location>
            <begin position="36"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="13">
        <location>
            <begin position="164"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="14">
        <location>
            <begin position="495"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="13">
        <location>
            <begin position="764"/>
            <end position="1000"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="404"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="408"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="447"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="463"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAI7750748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="1007" mass="109802" checksum="2DE0460B73DC0818" modified="2024-05-29" version="1">MASYTCNVNLNSSNGNFCFGYVSSDNEDDITGPNCVASFTGEKKAVADYNKSLVVAYRSSVHEGLVAGIGLGSTTFILFCSYALAVWFGAKMILERGYTGGDVLAVIFSVLTGSMSLGQTSPCLSAFAAGQAAAFKMFDTINRKPEIDTYDKKGRVLSDIHGDIELKDVYFRYPARPDEEIFSGFSLCVSSGTTAALVGQSGSGKSTVISLIERFYDPQQGEILIDNINLKEFQLKWIREKIGLVSQEPVLFTSSIKDNILYGKDGASMDEIRVAAELANAAKFIDKLPQGLDTMVGEHGTQLSGGQKQRIAIARAILKDPRILLLDEATSALDAESERVVQEALDRIMDNRTTVIVAHRLSTVRNADMIAVIHKGKMVEKGSHSELVQDPEGAYSRLIKLQEVNSGSEHHNSEDQDKSETEKELTKISSRKICRQRSPSSRDHSKSGSFGLSPQFNDPPPESMEKDIDEPSKQLPKVPLRRLAYLNKPEMLVLIMGSIVSIINGAIYPTFSILLSSMIKTFYEPPNKMKSDSKFWALMFVVLGVVSFVAYPGRSYLFAVAGSKLIKRIRSLCFEKVVNMEVGWFDKPENTSGAIGARLSADAANVRGLIGDSLAQIVQDSSSAAAGLVIAFAACWQLAFIILALFPLMTVNTYVQMQYLKGFSNAKCMYEEASQVANDAVGSIRTVASFCAEERVMELYRQKCECPKKTGIKQGLISGIGSIAPDTTKAKSSAVSVFAILDRESEIDPSDVSGLTLETVKGEIEIRHVSFKYPTRPDVEIFRDLCLTIRSGKIVALVGESGSGKSTVIQLLQRFYNPDSGCITLDGTELHKFQLKWLRLQIGLVSQEPVLFNDTIRANIAYGKDDATEVEILAASELANAHNFISGLHQGYNTIVGERGVQMSGGQKQRIAIARAIVKSPKILLLDEATSALDAESERVVQEALDRVMVNRSTVVVAHRLSTIKGADVIAVVNNGVIVEKGKHEELISINDGFYASLVALHVNSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GSX9</accession>
    <name>A0AAD5GSX9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Amine oxidase</fullName>
            <ecNumber evidence="8">1.4.3.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_019722</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>L-topaquinone</name>
            <dbReference type="ChEBI" id="CHEBI:79027"/>
        </cofactor>
        <cofactor evidence="8">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
        <text evidence="8">Contains 1 topaquinone per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>L-topaquinone</name>
            <dbReference type="ChEBI" id="CHEBI:79027"/>
        </cofactor>
        <text evidence="7">Contains 1 topaquinone per subunit.</text>
    </comment>
    <comment type="PTM">
        <text evidence="7 8">Topaquinone (TPQ) is generated by copper-dependent autoxidation of a specific tyrosyl residue.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the copper/topaquinone oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.4.3.-" evidence="8"/>
    <dbReference type="EMBL" id="JAMZMK010004125">
        <property type="protein sequence ID" value="KAI7754197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GSX9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008131">
        <property type="term" value="F:primary methylamine oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009308">
        <property type="term" value="P:amine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.20">
        <property type="entry name" value="Copper amine oxidase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000269">
        <property type="entry name" value="Cu_amine_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015798">
        <property type="entry name" value="Cu_amine_oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036460">
        <property type="entry name" value="Cu_amine_oxidase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016182">
        <property type="entry name" value="Cu_amine_oxidase_N-reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015800">
        <property type="entry name" value="Cu_amine_oxidase_N2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015802">
        <property type="entry name" value="Cu_amine_oxidase_N3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10638:SF69">
        <property type="entry name" value="AMINE OXIDASE [COPPER-CONTAINING] GAMMA 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10638">
        <property type="entry name" value="COPPER AMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01179">
        <property type="entry name" value="Cu_amine_oxid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02727">
        <property type="entry name" value="Cu_amine_oxidN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02728">
        <property type="entry name" value="Cu_amine_oxidN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49998">
        <property type="entry name" value="Amine oxidase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54416">
        <property type="entry name" value="Amine oxidase N-terminal region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0186">Copper</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 8" id="KW-0801">TPQ</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="5"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="domain" description="Copper amine oxidase N2-terminal" evidence="11">
        <location>
            <begin position="64"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="Copper amine oxidase N3-terminal" evidence="12">
        <location>
            <begin position="155"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="Copper amine oxidase catalytic" evidence="10">
        <location>
            <begin position="279"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="357"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate; via topaquinone" evidence="6">
        <location>
            <position position="445"/>
        </location>
    </feature>
    <feature type="modified residue" description="2',4',5'-topaquinone" evidence="7">
        <location>
            <position position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR600269-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR600269-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7754197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="614" mass="68780" checksum="8F9D45E76EAFEA38" modified="2024-05-29" version="1">MEKPYCIQIFIIFISIAVFIFGCSTSTTRQAFANTHRNTNIQSSPIIDTPVHNSNHHLTNTPLHPLDPLTVPEINKIRSILAAYEPFSLSFPSINTLSLDEPEKSQVLSWKKGDPLPPRKASITSVLDGQTHLLVVDLDLNVVTGHVVSIGSGYPMLTTEDQTTALEVLYKDLEFNKSIIARGVDFNDLVCGSSSPGWFGPDEEGKRIVKVQCFSGQNTPNYYMRPIEGLTATVDVDKREVVKITDTGRDIPIPKETNTDYLYKTQSQFPYNSETNPMSMEQPKGPSFTVENGHTVKWANWVFHIKPDIRAGMIISQAMIRAENGEYRSVMYKGFASEVFVPYMDPDQAWYFKTYMDVGEYGLGATAMPLLELNDCPRYSYYMDGVFASADGRPFIRPNIICIFERYAGDIGWRHSEIPAMGFDLRESRPKVTLVARMVASVGNYDYIFDWEFQTDGVIRIKVGLSGMLLVKGTPYENIYNISNTEDMTGTLVSENVIGVVHDHYISFYLDMDIDGPNNSFAEVNLVKEETSPGQSPRKSYLKAKRKVAKTENDAKIKLSLYDPSEFHVFNPARRSRLGNPTGYKIVPGGTAASLLDCDDTPQIRAAVTNNQES</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GSZ5</accession>
    <name>A0AAD5GSZ5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cyclin C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_008071</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005730">
        <property type="protein sequence ID" value="KAI7752199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GSZ5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004367">
        <property type="entry name" value="Cyclin_C-dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02984">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin C-terminal" evidence="2">
        <location>
            <begin position="16"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7752199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="116" mass="13693" checksum="9E440050788E72FA" modified="2024-05-29" version="1">MEADMLKALNYELGYPTVISFLRRYTKVTQEDYKTHNLWLEFMGYYLAELSLLEYGCLNFYPIWLAASVPFLQDLLSNQDHIRGQVVYTLVIFILLCSIKTPSLILSYCYRICLWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GT49</accession>
    <name>A0AAD5GT49_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_032507</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010003518">
        <property type="protein sequence ID" value="KAI7754437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GT49"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="261"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="261"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7754437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="418" mass="44775" checksum="DE68F4E491229078" modified="2024-05-29" version="1">MVIGTRSGKRQHVELSAAGALVCLPYDVRATPIDVGSSTPVFKAIKKRKRNFEINSDSDGDNFGDSDVAGSKLKSRPTQSWAIPLPDHLLTLLVFDYVCTLYNAIVLCNCVKTMVIGTPSGKRQHLELSAVGALVCLPYDVRVFKAIKKRKRNFEINSDSDGDNVGDSEVAGSKLKSRPTQSWLIYVDTFSVNGMSTKPAEYKKSLSFADEDAAETSKTLDTPTKRIMDVVTELGELYGVRAVSNVSVDVEGVNGHVESADVDSHEIRLGRDGSGEEDDSSDSGGENEWLLTRVGPVGKCYGGQPENEQSVPLTGIRSNLSAGVSKQSDPVPVDDAPNTKASKNVAMNTESASSFSLGLTQLGDNSPVEGKKHNEAVAEDLVVNVAKDVAGEKLSGDQMLRIMVRGALLATKILTPQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GTH0</accession>
    <name>A0AAD5GTH0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_029212</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005635">
        <property type="protein sequence ID" value="KAI7752679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GTH0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48183">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48183:SF1">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="17"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7752679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="65" mass="7669" checksum="2D9C8581B9A2598A" modified="2024-05-29" version="1">MGTRNFDVKKLLNDRKFWFASFLIAWAAALQGHMMWLQRQDTFKQKFGTLDDQNTDNQQDSNEGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GV22</accession>
    <name>A0AAD5GV22_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_020038</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005444">
        <property type="protein sequence ID" value="KAI7753386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GV22"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22160">
        <property type="entry name" value="F-box_AtFBL13-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053781">
        <property type="entry name" value="F-box_AtFBL13-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006566">
        <property type="entry name" value="FBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31639:SF333">
        <property type="entry name" value="F-BOX DOMAIN, FBD DOMAIN, LEUCINE-RICH REPEAT DOMAIN, L DOMAIN-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31639">
        <property type="entry name" value="F-BOX PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00579">
        <property type="entry name" value="FBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="6"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7753386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="354" mass="40393" checksum="94848F9E611E60A7" modified="2024-05-29" version="1">MVSAEEDILTNFPQDLINPILERLPVKEAVRTSVLSKKWRYRWTTMKTLDLYNQFSQQFLEIGGYQHNGFIRVISQIMTYHHGPILKFHLHIPKEINLDSFQEVDQWIFILRILVSRVESVDPPFEFQGFPNLQHINLLYVNFGGGTVINLPQLKILSLVSCRSVNKFNIKAEKLQALWVTGCPDANAMVLQLLHSEHLNVILAAEKFPKWLPHAVNCLEKLKFISFSFGDLNQLQGALCMLRNSPNLEVLRVTPTQMGPEADLGLTSTFLESPDCLDQTSLMLNYVEMTSLEGSRPELLFVKLLLHCSPHLEKMIIRPAATLDAEKRLNITKDVLMFSRASTKAKVAFLDPQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BKX2</accession>
    <name>A0AAD5BKX2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_016638</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ARG7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012013">
        <property type="protein sequence ID" value="KAI7725195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BKX2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009733">
        <property type="term" value="P:response to auxin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003676">
        <property type="entry name" value="SAUR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31929">
        <property type="entry name" value="SAUR-LIKE AUXIN-RESPONSIVE PROTEIN FAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02519">
        <property type="entry name" value="Auxin_inducible"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7725195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="99" mass="11077" checksum="AEA3A98B8A0FE969" modified="2024-05-29" version="1">MAIRMPRIVQARQILKRPLSNGSSTPESMGIPKGYFAVYVGEQEKKRFVVSVSLLSEPTFQDLLHRAEEEFGYNHPMGGLTIPCSEDVFMDLASRLGAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BL50</accession>
    <name>A0AAD5BL50_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Sec-independent protein translocase protein TATC, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_015943</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TatC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011971">
        <property type="protein sequence ID" value="KAI7725373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BL50"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033281">
        <property type="term" value="C:TAT protein transport complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009977">
        <property type="term" value="F:proton motive force dependent protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043953">
        <property type="term" value="P:protein transport by the Tat complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00902">
        <property type="entry name" value="TatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019820">
        <property type="entry name" value="Sec-indep_translocase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002033">
        <property type="entry name" value="TatC"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00945">
        <property type="entry name" value="tatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30371">
        <property type="entry name" value="SEC-INDEPENDENT PROTEIN TRANSLOCASE PROTEIN TATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30371:SF0">
        <property type="entry name" value="SEC-INDEPENDENT PROTEIN TRANSLOCASE PROTEIN TATC, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00902">
        <property type="entry name" value="TatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01840">
        <property type="entry name" value="TATCFAMILY"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01218">
        <property type="entry name" value="TATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="135"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="186"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="218"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="266"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="302"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="326"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7725373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="351" mass="38546" checksum="280AC918169ADF34" modified="2024-05-29" version="1">MGSTNTLIHNLNLNTTRSFNCVNSTTNHSNSLQINNRKHPLSFSCRRQRKISKVVCFALEDVRDMQTRLGIGGSGPVVEDRPEVVDDIQEDGLANTDSSANGSALYNFLYPDKELLPEDKEMTIFDHLEELRDRIFVSVLAVGAAIMGCFVYSKDLIMILEAPVRTQGVRFLQLAPGEFFFTTIKVSGYCGLLLGSPVILYEIIAFVLPGLTRSERKFLAPIVLGSSILFYTGIAFSYVVLTPAALNFFVSYAEGVVESLWSIDQYFEFVLVLMFSTGLSFQVPVIQLLLGQVGLVSGDQMLSIWRYVVVGAVVAAAIVTPSTDPLTQMLLAGPLLGLYFGGAWTVKLIGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BL77</accession>
    <name>A0AAD5BL77_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Flavin-containing monooxygenase</fullName>
            <ecNumber evidence="11">1.-.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_020254</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>indole-3-pyruvate + NADPH + O2 + H(+) = (indol-3-yl)acetate + CO2 + NADP(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:34331"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17640"/>
            <dbReference type="ChEBI" id="CHEBI:30854"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.14.13.168"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Plant hormone metabolism; auxin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the FMO family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.-.-.-" evidence="11"/>
    <dbReference type="EMBL" id="JAMZMK010011941">
        <property type="protein sequence ID" value="KAI7725432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BL77"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0103075">
        <property type="term" value="F:indole-3-pyruvate monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004499">
        <property type="term" value="F:N,N-dimethylaniline monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009851">
        <property type="term" value="P:auxin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000100">
        <property type="entry name" value="Flavin-containing monooxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050982">
        <property type="entry name" value="Auxin_biosynth/cation_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020946">
        <property type="entry name" value="Flavin_mOase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43539">
        <property type="entry name" value="FLAVIN-BINDING MONOOXYGENASE-LIKE PROTEIN (AFU_ORTHOLOGUE AFUA_4G09220)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43539:SF49">
        <property type="entry name" value="INDOLE-3-PYRUVATE MONOOXYGENASE YUCCA7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00743">
        <property type="entry name" value="FMO-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00469">
        <property type="entry name" value="PNDRDTASEII"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0073">Auxin biosynthesis</keyword>
    <keyword evidence="5 11" id="KW-0274">FAD</keyword>
    <keyword evidence="4 11" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8 11" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="7 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7725432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="404" mass="44908" checksum="AD57DCEA3DBBE3A6" modified="2024-05-29" version="1">MVQQTLDQSPLFSRRCIVVNGPVIIGAGPSGLAVGAGLKIQGVPFVILDRADCIASLWKNKTYDRLKLHLPKQFCQLPFFPFPPNFPDYPSKYQFIDYLEAYTKKFEINPRFNESVESAKYDKVCGLWRVQTVVHDCEIEYICRWLVVATGENAEKVIPQFEGLDEFGGHVMHACDYRSGESFQGKKVLVIGCGNSGMEVSLDLCHHNAFPSMVVRSSVHVLPREIAGKSTFELATSLMKWLPVKIVDNILLILARFSLGSLDKYGIKRPLIGPLELKNTKGKTPVLDIGTLQKIKSGNIQIVPGIKKFSRGHVELVNGTNLDIDSVILATGYCSNVPSWLKESDLFSREGMPTTSFTEGKCGLYAVGFTRRGLSGASLDAIRVSQDIAKIWNEETRPLCHSFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BL92</accession>
    <name>A0AAD5BL92_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_025414</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012015">
        <property type="protein sequence ID" value="KAI7725189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BL92"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.70:FF:000001">
        <property type="entry name" value="Cleavage stimulation factor subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.70">
        <property type="entry name" value="Transcription termination and cleavage factor, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025742">
        <property type="entry name" value="CSTF2_hinge"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026896">
        <property type="entry name" value="CSTF_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038192">
        <property type="entry name" value="CSTF_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47866">
        <property type="entry name" value="HYDROXYPROLINE-RICH GLYCOPROTEIN FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47866:SF2">
        <property type="entry name" value="HYDROXYPROLINE-RICH GLYCOPROTEIN FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14327">
        <property type="entry name" value="CSTF2_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14304">
        <property type="entry name" value="CSTF_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Cleavage stimulation factor subunit 2 hinge" evidence="6">
        <location>
            <begin position="6"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription termination and cleavage factor C-terminal" evidence="5">
        <location>
            <begin position="334"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="67"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="72"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="93"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="106"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="164"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="194"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="245"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="266"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF14304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF14327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7725189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="375" mass="40552" checksum="FDA8EF682B1015DB" modified="2024-05-29" version="1">MAGSGDGLSMNLAGMSKNQLYDIMSQMKTLIEQNQDQAKQILIQNPPLTKALFQAQIMLGMLQPPQEVPKVQPDLPQHAQQAAPPVQQPNAQTNPLPGQSSLRDQTSVPVPSSSISPSNPTLPSLPLQPPIQHSRSHINAQLAPPMPSPQSSNMAALPPQYSTQPPPSIIQPTMPPASVPLQQPLHISSTPHLPLQPPLPSQPRPPSMPAFPHQSYPHMGPNAGFQPQGASQLHHSQPVFHSGSRPPTSMGTPFSQGQPPLPNQPPSQTMYQGGSSHLGMDFNQMGTQPERGPNWMPENAAHLPGGPSQPFLPPHMSSASQPPRPASLTSDMEKALLQQVMSLTPEQINQLPPDQRNQVLQLQQMLRIPEKLDQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BMA1</accession>
    <name>A0AAD5BMA1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_029377</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PP2C family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="4"/>
    <dbReference type="EMBL" id="JAMZMK010011824">
        <property type="protein sequence ID" value="KAI7725925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BMA1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045926">
        <property type="term" value="P:negative regulation of growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009414">
        <property type="term" value="P:response to water deprivation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000038">
        <property type="entry name" value="Probable protein phosphatase 2C 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="13">
        <location>
            <begin position="58"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="18"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="23"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7725925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="360" mass="39924" checksum="80421A2CBD03EFD2" modified="2024-05-29" version="1">MGRFASMFYGLTKSFSPVRRSKGSAEDSGKETAEAMAKDAKKNDMMLRSSGSINVNKLVSVCSKRGEKGVNQDCCIVWEEFGCQKDMMFCGIFDGHGPWGHYVAKRVCDLMPSSLLSNWQEVLLEASLDPKKVDGFHLWKNSFIKTCASVDQDLQHSRKFDSFHSGTTALTVVRQGELLVIANVGDSRAILATTSEDGCLVPVQLTVDFKPNLPYEAKRIRERKGRVFCMEDEPGVHRVWLPHEDSPGLAMSRAFGDFCIKDFGLISVPEVTQRLITCKDQFLVLASDGVWDVISNKEAVEIVSSTVDKSKSAKRLVECAAHAWKRKRKGIAVDDISAVCLFFHSGSHSSRQIQLVATSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BMI5</accession>
    <name>A0AAD5BMI5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_022012</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011756">
        <property type="protein sequence ID" value="KAI7726095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BMI5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7726095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="253" mass="28848" checksum="EF6717A8827B2D3F" modified="2024-05-29" version="1" fragment="single">RTVLFQLSSNNPSLSKFKFISPNKICKTNSKTSQSQNRILSHLLHGFQVRESRDERRISGGDRLLHAEVSDCEETGRLAFIPESLHAPSGDWNENKYGCSIYLPFSDLEASINRIMMVNIPYHSEIVAGGNSKKTELNDCNTSCDQLRQRDRLLSTENEMFKEHVVRLEVKPCVQIASTVKYRHLYVEIKMAVEDYITNLLSMHWTKRRLLESFMLASGVDVSPLKLVKAIAEFLADDLINVANGSSNKYLFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BMJ4</accession>
    <name>A0AAD5BMJ4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_031007</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011744">
        <property type="protein sequence ID" value="KAI7726127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BMJ4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043226">
        <property type="term" value="C:organelle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000178">
        <property type="entry name" value="Calmodulin-2 A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003299">
        <property type="entry name" value="Calflagin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050145">
        <property type="entry name" value="Centrin_CML-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23050">
        <property type="entry name" value="CALCIUM BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01362">
        <property type="entry name" value="CALFLAGIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="22"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="58"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="95"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7726127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="161" mass="18283" checksum="7205F557A1EE5848" modified="2024-05-29" version="1" fragment="single">DCFGSSSRRNTPSGSHHGLTRQMKQELQEAFDMFDADSNGTIDAKELRNATRALGFEMTKEQIDEMMAEVDRNGSGAIDFDEFVYMMSDKICDRNNKHELTKAFNIIDHDKNGKISIPDIKNIAKELGVYLTDAQIQSMVEEADRDGEFLRVGYNMFKTIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BQN1</accession>
    <name>A0AAD5BQN1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_021349</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011667">
        <property type="protein sequence ID" value="KAI7726439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BQN1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000914">
        <property type="term" value="P:phragmoplast assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14010">
        <property type="entry name" value="STKc_ULK4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056981">
        <property type="entry name" value="HEAT_ULK4_RUNKEL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044591">
        <property type="entry name" value="RUK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46562">
        <property type="entry name" value="SERINE/THREONINE-KINASE ULK4-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46562:SF1">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE ULK4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23606">
        <property type="entry name" value="HEAT_ULK4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="4"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="288"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="384"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="306"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="349"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="384"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7726439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="1078" mass="120401" checksum="C201BA043FFC28B0" modified="2024-05-29" version="1">MNNYHIYEAIGQGKYSTVYKGRMKKSIEYFALKSVDKSHKSKVLQEVRILHSSDHPNVLKFHAWYETSAHLWLILEYCVGGDLKTLLEQDSKLPEDSVHDLARDLVRGLRYLHSKGVIYCDLKPSNILLDENGRTKLCDFGLSRKLSDISKTPSSLLPQAKRGTPCYMAPELFHDGGVHSYASDFWALGCVLYECYAGRPPFIGKEFTQLVKSILTDPTPSLPGNPSRPFANLVSSLLIKDPAERIQWSEICGHSYWNSKLTPPALPPQPAFDNMIELCYKPCLTERNLNKTPPRQRDNLKATVGVTENSNHNTPNRGVASGRKIQSKGSSNTKGVNLLRLSRIAKSNLQRENEKENYRRPLPNNNSEHEADVQIENTDMELDFNENNEDDTQDEPEMVTSTQSVDDENSSAPDNDLIASPVVTTPATDDTRRNDEECSLDHIEVPATPPSASPQPKIKKVIEGSDSVVNVSTVLWHASDLTVRPVMPSKKIDKGPGSEMALSLPFDAVPASDFAKLPKDQLEVVTKCIISILNGNTSVGEKQNVIKYLELLSCSVDAANILTNGQIMLVLVKMLRQSKASALRVQLVSLLGLLIRHSTEIDDDLANSGILGSLNEGLVDRQEKVRRFSMAALGELLFYISTKNDQPKAVIPPESPLKENRTSSGWQVPNTLISLVTSLLRKGEDDITQLYALRTIENISSQGGYWATRFTSPDVINNLCYIFRAPAKQETLRLTAGSCLVRLTRFNPPTIQQVMEKLTFKETANAISKGSPREQQICLNLLNMAMLGSNLFANIGRHLLPLIEDKSLVPNLISLIEHGGEVLRGKTLVFITLLCKNRKRWLAHFFFNVKLLTTVDRLVKEKCVYLQQCLEAFINGVVSIIPGLLDMIIADIKQLHGGKRHVQGGGLTGKAGGSKTNLPLFLVVLNLLCSSSFKRRVVDSQILQQLKVLIKLVESPFQGRDDFLLTLLRVLEAITEEGSIIEENHTTFVIEILPSLSVICKGSKDGDARFLCLKIWFEVVVNMLNEAPKDTRRLEVLKSVCRDHFVPMFPMLMEDEDPTPIYAQKLYDMLLEFNVIVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BRH2</accession>
    <name>A0AAD5BRH2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_021785</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MLO family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011506">
        <property type="protein sequence ID" value="KAI7726859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BRH2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004326">
        <property type="entry name" value="Mlo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31942:SF82">
        <property type="entry name" value="MLO PROTEIN HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31942">
        <property type="entry name" value="MLO-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03094">
        <property type="entry name" value="Mlo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0568">Pathogenesis-related protein</keyword>
    <keyword evidence="4" id="KW-0611">Plant defense</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="36"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="36"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="116"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7726859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="135" mass="15025" checksum="3D545DAD60C3D768" modified="2024-05-29" version="1">MCSYITFPLYALVTQMGTHMKKSIFEEQTTKALKKWQKAAKERKRLRDLGGGGGVGDTSKSGLMSPEHTPSRGSSPIHLLHGQNHRSSMAESEIDIPPSPRAYASETELSDMEASYHTQDQPKNLHQDFSFSRSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BS76</accession>
    <name>A0AAD5BS76_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="2">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_001045</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the peptidase C19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010011193">
        <property type="protein sequence ID" value="KAI7728527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BS76"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02661">
        <property type="entry name" value="Peptidase_C19E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000078">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF663">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="4">
        <location>
            <begin position="57"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="18"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="368"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="424"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="531"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="595"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="715"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="865"/>
            <end position="912"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="369"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="542"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="567"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="595"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="605"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="625"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="720"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="737"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="879"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="892"/>
            <end position="904"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7728527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="912" mass="100446" checksum="894FF3F7191CFC93" modified="2024-05-29" version="1">MESLAPFRRIEFNPAESPLTKNTNRVGSSGKKRDASELENGLDQELSFGITFRRIGAGLENLGNTCFLNSVLQCLTYTEPLAAYLQSGKHQVTCRKSGFCALCAIQKHVSRALQLSGRSLAPKDLVSNLRSISRTFRNSRQEDAHEYMVNLLESMHKCCLPNGVPIESQSAYDKSLVHKIFGGQLRSQVKCMQCNYCSNKFDPFLDLSLEILKADTLYKAFAQFTAKEQLDGGAKQYQCQQCKQKVKALKQLTVYKAPNVLTVHLKRFGCHMSGQKIDKKVHFGPTLDLKPFVTGPYDGDLKYTLYGVLVHAGWSTHSGHYYCFVRTSSGMWYSLDDNRVYQVSEKKVFEQKAYMLFYFKDTRNFPSKKTTDVHHKEKTVMNGAPSGSAAPRDTQMKTGLSNWSLNAQTPLKKTADVAQQKDNSIVMSGTPNGPDAPREAQVNTGLSNGSLNADISLATVGTERNCLSSVASKETQTNTILSNSSLNDQKSLVSGSTLSCSSAPKEAQMKTGLSDGSVNGQKSLAAVGTKENGLDPVAPNETQTNSSLSNGSLGGQKSSPITGATELEQTQTKETSESSAIAGTMIKAFTTGATELEQTQMKETSVSKRISEPSKTEDHTKEIGGGESSGGHSGGDGYVGATTALLKVSGLPQQERSMENKALGGITHNVELSDLPQKESSMEKKAIMGKPRKKVKNFLKCKLLSMKLSSNALLGTSLNQRKKKKHKRNKRVKNLAPENSVNEGSSSLVKSEAQETVKPLVDDGFKPRVIEDGSVLATSDFNSRSQSNSTESHQLENCRRSLSQKALIHMLTRGIDERTVPQWDEDESASSQIFREERPNFAIGYTGDKWDEEYDRGKRKKVRISKTEFDGKNPFQDIANERLKSHSEKQNVSKRKNKHFKKPRSGNGPYRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BSB2</accession>
    <name>A0AAD5BSB2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_002212</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011186">
        <property type="protein sequence ID" value="KAI7728615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BSB2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7728615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="64" mass="7183" checksum="ACE4B6C49582FBD2" modified="2024-05-29" version="1" fragment="single">WVCILRGELAKGLCGFERRGYADLGTDRLQSDELMMKGIVSKCFCCLKVARLAIHSPLENWGHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BTI3</accession>
    <name>A0AAD5BTI3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NPH3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_020484</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NPH3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011263">
        <property type="protein sequence ID" value="KAI7728151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BTI3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043454">
        <property type="entry name" value="NPH3/RPT2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027356">
        <property type="entry name" value="NPH3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32370">
        <property type="entry name" value="OS12G0117600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03000">
        <property type="entry name" value="NPH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51649">
        <property type="entry name" value="NPH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="NPH3" evidence="5">
        <location>
            <begin position="205"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="358"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="529"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="365"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="533"/>
            <end position="557"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7728151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="616" mass="68694" checksum="DB060AA3788F6D41" modified="2024-05-29" version="1">MKFMKLGSRPDTFYNSESVRSVSSEVSSDLVVQVNGTRYLLHKFPLLSKCLRLQKLCSESIESTQHQIIQLPDFPGGTESFELCAKFCYGITITLSAYNIVSARCAAEYLQMTEEVEKGNLVYKLDVFLNSCILNGWKDSIITLQTTKSFQLWSEDLGITSRCIEAIAAKVLSNPLKVSLSHSYSRRGRDRDDISVNGSKTTSKGWWAEDLSELGIDLYWRTMIALKSGGKVPSNLIGDALRIYASKWLPNISRNPENGETITNSNSKSRLLLESIISLLPTERASVSCSFLLKLLKAANILRASSSSKSELARRIGIQLDEATVSDLLIPNSSNDMTYDVEVVFTILEQFMLQSQSPPTSPVHIKGRFERHRRSRSANNGDFELQESRRSSSASHSSKIKVAKLIDGYLKEIGKDVNLPLSKFTALAEAIPDFARLDHDDLYKAIDIYLKSHPNLNKTERKRLCRTLDCKKLSMEVCMHAAQNELLPLRVVVQVLFFEQARAAMAGGQLTDLPSNIKALLAAQDDASRAPGSLSTSRSMVQPEDQWSVSGLKSPSSNISTLRMKLAENDDLDENFHDGISKAHSSKVKQLCSLPTRPKRMFSKLWLTNRSASEKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BTR8</accession>
    <name>A0AAD5BTR8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">AUGMIN subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_030608</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Spindle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HAUS1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011274">
        <property type="protein sequence ID" value="KAI7728059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BTR8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070652">
        <property type="term" value="C:HAUS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051225">
        <property type="term" value="P:spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026243">
        <property type="entry name" value="HAUS1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31570">
        <property type="entry name" value="HAUS AUGMIN-LIKE COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31570:SF1">
        <property type="entry name" value="HAUS AUGMIN-LIKE COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25762">
        <property type="entry name" value="HAUS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02087">
        <property type="entry name" value="HAUSAUGMINL1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7728059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="299" mass="33465" checksum="D4706B095F6ECBE8" modified="2024-05-29" version="1">MSDTPANELSKTINGSQFDANTIADIKSWLSSQFDAAGKDHVPDFEYTSRTIQHLHNLATVSQSKTQAASIIANDFRLKATEYRAQAARIREILENVGLAQEGLPANVVASSQVLANVANLLNIRDTELSSFLVAMGDVSLRKTGVEDKRAKVQKESKILLDYTRKAIARLTYLKRTLAQLEDDVAPCETQMDGWKTNLAILVSKERQYLQQYSNYKAMLNRVGYTPEISHGMLVEMAEHRKDLEKKTKPILDTLRSYQDLPPDKALAALAIEDKKRQYNAAEKYLEDVLQSALATSED</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BUR3</accession>
    <name>A0AAD5BUR3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_029428</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010909">
        <property type="protein sequence ID" value="KAI7729825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BUR3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016125">
        <property type="term" value="P:sterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11043">
        <property type="entry name" value="CYP90-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000022">
        <property type="entry name" value="Taxadiene 5-alpha hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24286">
        <property type="entry name" value="CYTOCHROME P450 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24286:SF347">
        <property type="entry name" value="CYTOCHROME P450-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0349">Heme</keyword>
    <keyword evidence="8 10 11" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="7 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="binding site" description="axial binding residue" evidence="10">
        <location>
            <position position="424"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7729825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="478" mass="54266" checksum="F783D62EEF411A2F" modified="2024-05-29" version="1">MDITLSISLSVLVVLSFSLFSFLLVFLKRASSSPNTPPGSIGWPIVGESIEFGLSGPPKFIKTRMEKFSANVFKTSLLGEKMAFFCGPQGNKFVFANENKVLASWWPKSATKVLLFTEVIDENQKVVAPLLANSIHDILKPEALKQYVPLMDSMARKHMETEWIPYKEVKVYHLSQKYTFALSCKLFMDEDDEDKVAKILENFNTVTNGMISIPIDFPGTAYNRAVKGGKILRDGLMKIISTRRKELTENKAIVRDDILSKMLLVTNEHGRLMSEKEISNNIIGLLVASYETSSVAITFVLKHLAENPHVYEQVREEQMMIAKSKQDELLTWEDIEKMKYSWNVARESIRLAPPGQGAFREALTDFKYAGYTIPKGWKIFWNVNTTHSDPKYFPDPQKFDPSRFEGSGPVPYSFVPFGGGPKMCPGKEYARLEILVFMHNFVKNFKLKTLIPNEKIIYRSTPVPEKGLPVHLYPHGKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BUT0</accession>
    <name>A0AAD5BUT0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ion transport domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_022051</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010844">
        <property type="protein sequence ID" value="KAI7730083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BUT0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034702">
        <property type="term" value="C:monoatomic ion channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045319">
        <property type="entry name" value="KAT/AKT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45743">
        <property type="entry name" value="POTASSIUM CHANNEL AKT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45743:SF21">
        <property type="entry name" value="POTASSIUM CHANNEL AKT2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0407">Ion channel</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0630">Potassium</keyword>
    <keyword evidence="4" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="2" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <keyword evidence="5" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="29"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="88"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="11">
        <location>
            <begin position="1"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7730083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="122" mass="14321" checksum="2001F67C072615AA" modified="2024-05-29" version="1" fragment="single">MVVMVVYSAWTYPFEVAFLKTSTQSHTQLYIADSIVDLFFVVDIVLTFFVAYFDSTTHLLVRDHKSIATRYLSTWFVMDFASTLPFELISYLFTGKHGMGLSYTVIGLLRFWRLRRVKKFFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BX57</accession>
    <name>A0AAD5BX57_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_020007</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in the regulation of shoot development and salicylic acid (SA) homeostasis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the iron/ascorbate-dependent oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010852">
        <property type="protein sequence ID" value="KAI7730056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BX57"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.330:FF:000015">
        <property type="entry name" value="Protein DMR6-LIKE OXYGENASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050295">
        <property type="entry name" value="Plant_2OG-oxidoreductases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47991">
        <property type="entry name" value="OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00682">
        <property type="entry name" value="IPNSYNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9" id="KW-0408">Iron</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="10">
        <location>
            <begin position="214"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00059922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7730056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="352" mass="40392" checksum="94700211B30FBFE4" modified="2024-05-29" version="1" fragment="single">MSMQAEQHMYQQGVQHLCDNGLTQVPNKYILPEPERPLNVVDDGCINLPIIDFAQLQGENRPRVLASLSKACQEFGFFQFKVEVKLCELWMWMQVINHGLGDEAIGKMIDVSKRFFELPFEERQRYMSSDLHAPVRYGTSFNQNNDKVFCWRDFLKLSCHSMQDYSSLWPSSPLDLREAMGVYSTKTQHLYERVMEAILESLGVTNETAIFEQGSQLMMVNWYPACPQPQLTLGLPPHSDYGLLTLLLQDQVEGLQIQHNDKWVTVKPIPNSFVVNIGDQFEIFSNGRYKSVVHRVAVNSMRSRLSVASLHSLNANYNVTPSPILIDDSNPRLYQDTNYADFVDYLSSSDFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BXM4</accession>
    <name>A0AAD5BXM4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Post-GPI attachment to proteins factor 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_011350</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in the lipid remodeling steps of GPI-anchor maturation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Golgi apparatus membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the PGAP3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010541">
        <property type="protein sequence ID" value="KAI7731114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BXM4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007217">
        <property type="entry name" value="Per1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13148">
        <property type="entry name" value="PER1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13148:SF0">
        <property type="entry name" value="POST-GPI ATTACHMENT TO PROTEINS FACTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04080">
        <property type="entry name" value="Per1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="2 7" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="156"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="191"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="225"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="255"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="279"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="312"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="340"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7731114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="374" mass="43044" checksum="76FED90F09D5C378" modified="2024-05-29" version="1">MLLLDGLGALPAVLTDFEEKFVIREKQLPDQQMVRCYWISLFLTFSCCFGVLHASIGDADPSYRSCINDCEITGCVGDICFPHCNFSSDGASVDGPWYMQEPLYLRWKQWDCHSDCKYHCMVNREKDRAASGYEPVKYHGKWPFKRVFGIQEPASVVFSALNLAMHFHGWLSFFILLHYKLPVKPDKKPYYDYAGLWHLYGLLALNSWFWSAVFHSRDVELTERLDYFSAIALLGYSLIVAILRSFNVRLEAARVMVSAPLLAFVTTHILYLNNYKLDYGWNMKVCVAMGVAQLLIWAIWGGVSGHPSRWKLWFVIVTGALAMLLEIYDFPPYEGFVDAHAAWHATTIPLTYLWWSFVKDDAEFRTSVLLKKAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BYE0</accession>
    <name>A0AAD5BYE0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_023050</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010682">
        <property type="protein sequence ID" value="KAI7730686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BYE0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000554">
        <property type="entry name" value="protein indeterminate-domain 12-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000131">
        <property type="entry name" value="protein indeterminate-domain 5, chloroplastic-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055187">
        <property type="entry name" value="C2CH-3rd_BIRD-IDD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055185">
        <property type="entry name" value="C2CH-4th_BIRD-IDD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055186">
        <property type="entry name" value="C2H2-2nd_BIRD-IDD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031140">
        <property type="entry name" value="IDD1-16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10593">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE RIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10593:SF163">
        <property type="entry name" value="ZINC FINGER C2H2-TYPE_INTEGRASE DNA-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22995">
        <property type="entry name" value="C2CH-3rd_BIRD-IDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22992">
        <property type="entry name" value="C2CH-4th_BIRD-IDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22996">
        <property type="entry name" value="C2H2-2nd_BIRD-IDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="62"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7730686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="451" mass="49869" checksum="BF396687E829AA76" modified="2024-05-29" version="1">MSGEDLISSPSSLSAFIQDPIANPNPNSTNSSKRKRNLPGTPDPEAEVIALSPRSLMATNQFVCEICHKGFQRDQNLQLHRRGHNLPWKLKQRNKLEVVKKKVYICPEVSCVHHDPSRALGDLTGVKKHFSRKHGEKKWKCEKCSKKYAVQSDWKAHSKICGTREYKCDCGTIFSRKDSFITHRAFCDALTEENSRMASFPIMSSSANLNFRHDLMTMNGGGSGNNGMRFPGMFGSGMLGVNLDPNSDKPRLPIWLDQNVNEPHLENPSSASFLGPSSSTNNNNNNNGGMLPLDMVQWLNGSQEPVAGPYSGLYLKEEEGGKGEMQFNSMYNYTTSTAATLQPPGHMSATALLQKAAQMGSTRSSTNLDDTTGFGLMGTSLSNFDSSTHDHNGDGLMMMMNSTTSKRINGNGVNESHDSDLTRDFLGVGENERRSFNLQQKMFKFNSSMEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BZV0</accession>
    <name>A0AAD5BZV0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_024201</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010212">
        <property type="protein sequence ID" value="KAI7732482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BZV0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019396">
        <property type="entry name" value="TM_Fragile-X-F-assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46859:SF3">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46859">
        <property type="entry name" value="TRANSMEMBRANE FRAGILE-X-F-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10269">
        <property type="entry name" value="Tmemb_185A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7732482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="105" mass="11971" checksum="F78B4BBE66D2B3D8" modified="2024-05-29" version="1">MLVRNNMSWRRVANSLQAFSAHTALFCFTLLLVLKLDHLVSFSWWVVFFPLFLFHAVVARGRFSLPAPSVPHGREWAPCHAVVATPLLIAFELLLCIFLENTYGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C1P6</accession>
    <name>A0AAD5C1P6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Germin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">M8C21_003581</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">AA19_3_7</strain>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May play a role in plant defense. Probably has no oxalate oxidase activity even if the active site is conserved.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Oligomer (believed to be a pentamer but probably hexamer).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Secreted</location>
            <location evidence="2 13">Extracellular space</location>
            <location evidence="2 13">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the germin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010004">
        <property type="protein sequence ID" value="KAI7733427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C1P6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02241">
        <property type="entry name" value="cupin_OxOx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000098">
        <property type="entry name" value="Germin-like protein 9-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006045">
        <property type="entry name" value="Cupin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001929">
        <property type="entry name" value="Germin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31238">
        <property type="entry name" value="GERMIN-LIKE PROTEIN SUBFAMILY 3 MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00190">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00325">
        <property type="entry name" value="GERMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00835">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13" id="KW-0052">Apoplast</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 11 13" id="KW-0464">Manganese</keyword>
    <keyword evidence="7 11 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041781993" description="Germin-like protein" evidence="13">
        <location>
            <begin position="17"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="Cupin type-1" evidence="14">
        <location>
            <begin position="53"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU366015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAI7733427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="194" mass="21114" checksum="048CF1B9EC771D82" modified="2024-05-29" version="1">MVYMFAFAILCITTSGDPNILTDFVTLNNTIDANYFTFIGMRQLVGGEYPTTFKALKVSMNEFPTLNGQSVSYAILEFPVGSINPVHIHPRASELMFLVSGSLQVGFVDTSNKLYKQSLQVGDIFVFPKGLLHFQYNNDTKEPALAISAFGSANAGTQSIPASVFNTSIFGGILDASFHATRATIRKIEHGLEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C2U6</accession>
    <name>A0AAD5C2U6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_021693</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009908">
        <property type="protein sequence ID" value="KAI7733775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C2U6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080030">
        <property type="term" value="F:methyl indole-3-acetate esterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080032">
        <property type="term" value="F:methyl jasmonate esterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080031">
        <property type="term" value="F:methyl salicylate esterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009694">
        <property type="term" value="P:jasmonic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009696">
        <property type="term" value="P:salicylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000051">
        <property type="entry name" value="(S)-hydroxynitrile lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045889">
        <property type="entry name" value="MES/HNL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10992:SF943">
        <property type="entry name" value="METHYLESTERASE 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10992">
        <property type="entry name" value="METHYLESTERASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="21"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7733775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="271" mass="30706" checksum="6C9293FB4807E0AF" modified="2024-05-29" version="1" fragment="single">IKSLFLFEHMPTAKNNPRKHLVLAHGLGHGAWCWYKVVTQLKSVGHKVSAVDLGGSGVHPSRLEEIATFSDYIQPLLQFLESLSADERVVLVGHSYGGLAISVAMERFPHIVSAAVFITAYMPNCRDPPALQMTQYFKNLKPEMYMDCRFTFKDGSLTSAELGENYMANMMYQKCQPEDMALAKMLIRPSQLFLEDMSKDSLLTKEKYGSISRVYVVCEGDRVMDEEFQRFVIKDSPPDEVKSFPGVGHMIMLSNSKDISLYLQEILDRYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C427</accession>
    <name>A0AAD5C427_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FBD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_015429</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009660">
        <property type="protein sequence ID" value="KAI7734722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C427"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006566">
        <property type="entry name" value="FBD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08387">
        <property type="entry name" value="FBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00579">
        <property type="entry name" value="FBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FBD" evidence="1">
        <location>
            <begin position="51"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7734722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="117" mass="13692" checksum="14408A387F671669" modified="2024-05-29" version="1" fragment="single">MCFADDHGLTFLAVLIKCSPNLEKIELEINTGHSCCYENEICCGKLEEYPDLWLESLKELEIRFFRNLKREMEFVKFILARSPKLMKVNIRSYVEKNEESDMLKDLLQAPRASLQSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C4I1</accession>
    <name>A0AAD5C4I1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">60S acidic ribosomal protein P1-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_004962</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Plays an important role in the elongation step of protein synthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">P1 and P2 exist as dimers at the large ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic ribosomal protein P1/P2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009569">
        <property type="protein sequence ID" value="KAI7735093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C4I1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030295">
        <property type="term" value="F:protein kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043021">
        <property type="term" value="F:ribonucleoprotein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006414">
        <property type="term" value="P:translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05831">
        <property type="entry name" value="Ribosomal_P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1410:FF:000001">
        <property type="entry name" value="60S acidic ribosomal protein P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01478">
        <property type="entry name" value="Ribosomal_L12_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038716">
        <property type="entry name" value="P1/P2_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027534">
        <property type="entry name" value="Ribosomal_P1/P2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45696">
        <property type="entry name" value="60S ACIDIC RIBOSOMAL PROTEIN P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45696:SF10">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00428">
        <property type="entry name" value="Ribosomal_60s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="76"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="76"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7735093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="110" mass="11500" checksum="D8877E0FDFB0BF79" modified="2024-05-29" version="1">MSIGELACTYACLILTDEGIPITAEKIATLLKASNVECESYWPTLFSKLAEKKNINDLILNVGAGGGVGGAPAAAAPTVRGATEAAPPAEEKKEEIEEESEEEFGLGLFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C5T1</accession>
    <name>A0AAD5C5T1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Hydroxyproline-rich glycoprotein family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_015409</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009439">
        <property type="protein sequence ID" value="KAI7735752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C5T1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040420">
        <property type="entry name" value="At1g76660-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31798">
        <property type="entry name" value="HYDROXYPROLINE-RICH GLYCOPROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31798:SF10">
        <property type="entry name" value="OS02G0822000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="162"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="222"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="233"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7735752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="444" mass="47404" checksum="CE6B244054F22FDC" modified="2024-05-29" version="1">MNMSSINNNNNINSVETVNAAATAIVSAESRVQPTSVQKRRWGGCWSLYSCFGSYKHSKQISHALLAPESAPSGPAPSSLTQNPNNSNAIIFPFIAPPSSPASFLQSDPPSASYSPVGLLSFKSLSQNPNSQGVPSSIFTVGPYAYETQLVSPPVFSTYTTEPSTASFTPPPESVHATTPSSPEVPFAQLLTSSLARARRHSMGSNQKFPFSQYEFQPYTGSPGGSVLSPGSTIPNSGTSSPFPDKQPRVLKLRVGDDPKFIGYEYFSTRKWGSRLGSESATPNGVSGSATPHGYGLGSRVGSGSLTPNGGGEMYLMESQISEVASLANSATSSPKGETLVDHRVSFDLTHLDVMSGAANNLEASFRTSSGRKTVSNKTDNLCEYCVNKKNSSEILEAPKEKECQCKRASVSRDFNFDNTNGVELGSQNNWTFFPMLQPEVKPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C5Y7</accession>
    <name>A0AAD5C5Y7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Carboxypeptidase</fullName>
            <ecNumber evidence="10">3.4.16.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_022373</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Probable carboxypeptidase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the peptidase S10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.-" evidence="10"/>
    <dbReference type="EMBL" id="JAMZMK010009398">
        <property type="protein sequence ID" value="KAI7735961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C5Y7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11320:FF:000003">
        <property type="entry name" value="Carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000013">
        <property type="entry name" value="Carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11320">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033124">
        <property type="entry name" value="Ser_caboxypep_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018202">
        <property type="entry name" value="Ser_caboxypep_ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF198">
        <property type="entry name" value="SERINE CARBOXYPEPTIDASE-LIKE 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00560">
        <property type="entry name" value="CARBOXYPEPT_SER_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00131">
        <property type="entry name" value="CARBOXYPEPT_SER_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 10" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041773851" description="Carboxypeptidase" evidence="10">
        <location>
            <begin position="21"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7735961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="460" mass="52034" checksum="24148C20AC6D4FCB" modified="2024-05-29" version="1">MAYNSFFLFMFCFTFAIVIGACNSLSDQEKDRIVELPGQPSNVDFAQYSGYVTVNQESGRALFYWLTESPESRDPKSRPLLLWLNGGPGCSSVAYGAAEEIGPLHINSDGKSLFTNPYSWNKLANLLFLESPAGVGYSYSNTTSDLYTFGDAKTAEDSYAFLVNWFERFPQYKHRDFYIAGESYAGHYVPQLSQLIYERNKGVKNPVINFKGFMVGNAVTDDYNDYVGTFEYWWTHGLISDTTYKSLQKSCEGSSSEHPPIECIRALSTAEMEQGRIDPYSIYTKPCGYTSSLKRRGRYPWMRGAYDPCTENYANQYFNLPEVQKAFHANITKVSYPWKTCSDIVGGYWTDSPLSMLPIYKELIAAGLRVWVFSGDTDSVVPLTATRYSIDALNLPTIANWYPWYDNGKVAGWSQIYKGLTFVTVTGAGHEVPLHRPRQSFILFRSFLENKAMEGSQNSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C823</accession>
    <name>A0AAD5C823_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Lysine-specific demethylase JMJ25-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_022733</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the JARID1 histone demethylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009328">
        <property type="protein sequence ID" value="KAI7736283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C823"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000118">
        <property type="term" value="C:histone deacetylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032454">
        <property type="term" value="F:histone H3K9 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045109">
        <property type="entry name" value="LSDs-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014977">
        <property type="entry name" value="WRC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12549">
        <property type="entry name" value="JMJC DOMAIN-CONTAINING HISTONE DEMETHYLATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12549:SF67">
        <property type="entry name" value="TRANSCRIPTION FACTOR JUMONJI_ASPARTYL BETA-HYDROXYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02373">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08879">
        <property type="entry name" value="WRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51667">
        <property type="entry name" value="WRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="WRC" evidence="10">
        <location>
            <begin position="8"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="8">
        <location>
            <begin position="233"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="JmjC" evidence="9">
        <location>
            <begin position="602"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="693"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="10"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="46"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="96"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="712"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7736283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="940" mass="106016" checksum="8FE3824005B904A2" modified="2024-05-29" version="1" fragment="single">MQQNSAELPDDQRCKRNDGRQWRCRRPVVPGRTLCDAHFRQGRLRQNKEPVPDNLKLDRAKTRKHQQNPNDNLGSSRKRANTEEDSSDKTSNLSTKRPKRGEKKAKLKAKSSVDISEDLDDALKKMNLKKGDLQLDLIRGCLNRQVEKKKGKQPHKEDIVKELKYGRLEISQASPSTTPVAVSKVKVGAPVSGSAPTRFFRSKNIDPVPIATMQVLPNIKANVKASTKKCHWCRMCSYRVLIKCLTCKKHFFCEDCIRERAYDKAEVKKQCPICCGTCSCKACLRGKSKEVKTKDLLVYSGEEKFDKSQQLLYMISLLLPLLEQINQEKDIEMDIEAKIKGKDHSELQIQEVTGSPKQQCCGFCKACIVDVHRSCGNCSYVLCLSCCRDFREERLHGGLLWNWKTNEDGGIECPPKNLGGCGDGVLGLFCHPPINWTKDIEKSAKEIVCNPKYTTFFDFDSSHCGLCDGNDKNEGESEINKGLYFSIKEDLGDKNLGHFTKHWVRGQPLIIQDVIKSDVELSWDPLFMFFTYLDKSVKSRNDKEVKLKNCLDWCDVETGRQQIFMGGKTHANVWHEKLKFKVWLSSGIFQEHFPSHYAAVMNALPLQQYVNPLTGVLNLAANVPPESQNVDLGPFVYISYGRPEDVTGGDFLTKLCYHAYDTVNILVHATELHISEKRLNKVKILLNKYSSPDHNESLKKSKNKNKIVEASGKSSFSSEVTQQSDLADTNTNGELTQTPNDGDACVFSDDSSNCDSDDEDLCHDEYGSSSGSEGKVVDTHGAQWDVFRREDVEKLVEFLRKYSSELNSSYCSPKKVVHPALDEIFYLDAFHKKYLKEEFGVEPWTFEQNIGEAVIIPAGCPYQVKKTKSCVNVVLESVSPESASVGIKLSDEIRQLPVNHKAKGKMLDIKKMTIHSVNAAVEEFNKVSQGVEETDKVLQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C840</accession>
    <name>A0AAD5C840_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF4005 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_027243</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the IQD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009151">
        <property type="protein sequence ID" value="KAI7736967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C840"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025064">
        <property type="entry name" value="DUF4005"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32295">
        <property type="entry name" value="IQ-DOMAIN 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32295:SF292">
        <property type="entry name" value="PROTEIN IQ-DOMAIN 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13178">
        <property type="entry name" value="DUF4005"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4005" evidence="4">
        <location>
            <begin position="273"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="218"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="324"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="222"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="241"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7736967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="419" mass="48022" checksum="3C7A935A328B0CC6" modified="2024-05-29" version="1" fragment="single">KWLNVVKRAFSPHKSKTKTQDDDDDNKREKRRWLFRKSSTTLVNINISTNDNTPQEENHAIFTAEEAATTANASVSVKHHFAATLIQTYFRGYLALLRVQSRVHDQRSRLSQDGRRRSMMSGNTSFCESRYLEDIRRRKSMSRDGTCIPDDWSDRPRNLEELDAKIHNRKMSNSNLSAMDEKELEETASWLDQWIRAKQWENQRTSSRASFERRDSIKTIEIDSSTPSSRSGVHKPPIPNSPSRRSSYSPSPGHQPITPSPVKTIPLQIRSASPRCFKEERSYLNANIQSLRLTPRVMESVCRYSTCANDMTVPNYMAATESAKARIRSQSTPRHRPSTPERDRLGSVKKRLAYPVPDPCDNSCDTYNYCDYGYNLRSPSFKSVQVGHVGMGQQWYSADSTNGGEISPCSTTDLRRRLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C851</accession>
    <name>A0AAD5C851_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">S1 motif domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_005395</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the crooked-neck family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009150">
        <property type="protein sequence ID" value="KAI7736982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C851"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000065">
        <property type="entry name" value="Programmed cell death 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000155">
        <property type="entry name" value="rRNA biogenesis protein RRP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000159">
        <property type="entry name" value="rRNA biogenesis protein rrp5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055433">
        <property type="entry name" value="HAT_Syf1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057300">
        <property type="entry name" value="OB_Rrp5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045209">
        <property type="entry name" value="Rrp5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057301">
        <property type="entry name" value="Rrp5_OB_4th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057302">
        <property type="entry name" value="Rrp5_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008847">
        <property type="entry name" value="Suf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23270">
        <property type="entry name" value="PROGRAMMED CELL DEATH PROTEIN 11 PRE-RRNA PROCESSING PROTEIN RRP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23270:SF10">
        <property type="entry name" value="PROTEIN RRP5 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23233">
        <property type="entry name" value="HAT_Syf1_CNRKL1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24682">
        <property type="entry name" value="OB_RRP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24685">
        <property type="entry name" value="OB_RRP5_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23459">
        <property type="entry name" value="S1_RRP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05843">
        <property type="entry name" value="Suf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="5" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="S1 motif" evidence="10">
        <location>
            <begin position="59"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="10">
        <location>
            <begin position="156"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="10">
        <location>
            <begin position="334"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="10">
        <location>
            <begin position="441"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="10">
        <location>
            <begin position="539"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="10">
        <location>
            <begin position="628"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="702"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="720"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="732"/>
            <end position="742"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7736982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="1090" mass="121832" checksum="625D983E123A5593" modified="2024-05-29" version="1">MMETSILSLSVMPKSKLNCSSTLVNARILFVDPSTRAVGLTLNPHLVHNNAPPSLVKIGDIFDDSKVVRVDRGSGLLLEIPSAPIPTPAYVNVSDLSDKEIKKWEKSFKEGSLVRVRVSGFRLLEGLATGVLKIAKLHYSGMKSSSLKWVKDFPIGNVIEGTVHEVKDSGVVISFQKYNDVFGFVTHHQCGGSTMDIGSTIKAAILDLAKMDRLVDLSLKAEFVNRNTEDTNSRTPKKMRKRPAHKDLEVNQTVNAVVEIVLSILDAKYALGYASLYDYNTQAFQPKQFVNGQRVTASVMALPDSSTADRLLLLLKSNSEATETSSAKRAKKKSSYDVGSSVQAEAKIKPLELKLKFGSGLHGRIHITEASDGNISEKPFSNYKIGQTLTAKIVSKSKAENSSNLRWELSIRPSVLAGGLLANGASMTDDSEILTSHICEGAIVGGRISKILPGVSGLIVQIDPRLSGKVHFTELTDSWISNPLSGYHEGQFVKCKVLDIGRSGTGAVHVDLSLRSSLVELNGGSGSNRYEKIEDLNPDMVIEGYVKNITPKGCFIMLSRKLDAKILISNLSDDFVSNPEEEFPIGKLVTGRVLSLEPLSKRIEVSLRKTSGSKEPKADGEFSSLHVGDIISGRVKRIESYGLFITIDQSKSVGLCHVSELPEDNTDNLETKYKVHERVKAKVLKIDEERRRISLGMKESYFDTNTDIGSPADHGSDLSNESDSESDDGTPSEFQKSLILSGNGNHPVLAEVESRASVLPLDVPLDETEDSPMDDDAGAQNLEVPDNVTKTSENKVRRTKKKEMEEREREIRAAEERLLQKDVPRNADDYEKLIRSSPNSSFIWLKYMAFLLSLNEVDKARSTAERALRTINIREESEKLNVWIAYFNLENEFGNPPEDAVVKVFQRALQVCDPKKIHFALLGMYERTNQDKLADELLEKMIRKFKHSCKVWLRRIQRVLKQNEDMVQSIVKRALICLPKHKHIKFITQTAISEFKSGVPDRGRSLFEGMLREYPKRTDLWSVYLDQEIRLDDEAVIRSLFERAICLELPLKKMKFLFKKYLEFEKSKGDEERIEYVKAEALKYMEKTRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C931</accession>
    <name>A0AAD5C931_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Disease resistance R13L4/SHOC-2-like LRR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">M8C21_028825</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">AA19_3_7</strain>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RLP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009060">
        <property type="protein sequence ID" value="KAI7737362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C931"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000041">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000213">
        <property type="entry name" value="Tyrosine-sulfated glycopeptide receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046956">
        <property type="entry name" value="RLP23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48061">
        <property type="entry name" value="LEUCINE-RICH REPEAT RECEPTOR PROTEIN KINASE EMS1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48061:SF2">
        <property type="entry name" value="RECEPTOR LIKE PROTEIN 30-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 13" id="KW-0732">Signal</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041959183" description="Disease resistance R13L4/SHOC-2-like LRR domain-containing protein" evidence="13">
        <location>
            <begin position="22"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="845"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="14">
        <location>
            <begin position="374"/>
            <end position="600"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAI7737362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="889" mass="98564" checksum="4DCA526B1919B08A" modified="2024-05-29" version="1">MGNSILLLFLLSLFFISSTSSSSIKTTPKCSVQQTHALLLFKQNLSSINHTCYDDTCEYWLGSGYKPIMMNWNTSTDCCDWNGVTCDHSTGDVISLDLSCGMLQGTIHPNTTLLHLPHLQRLNLAFNDFINSQLPPDIGKLSNSLTHLNISYSRFTGQVPTDISYLHKLVSLDLSSNYIDLEPHVFINLLQNSTFMKQLSLAYVYISSVLPTNLNISSSSSLKLLNLHSTGLQGNLPHNIFSLQSLENIDLSYNSLTGHIPSEISLLPKLVSLDLSWNTGIDNLLIQHHIFHTLLTNFTFLRDLRLDGVNIGLVLPTYLNISSSLISLHLSSTNLQGKLPDNIFNLEYLEELDLSWNNNLSGPLPKVNTSSSIPLSWLGISDTSLSGEIPTSIGHHKSLEHLILSNCGLNGSLPKSLGNLKYLESLDLSNNKFQGNMPLELFSLQSLKGLSLASNQLTGQIDVLDNGPSLQTLQRLSNLTYIDLSHNNFRGDWELDALLSSLPNLYTLIISDSGVSITTSNASNHYVKTGFRRLSLASCSLKVFPVFIKAMTNLEHLDLSNSAIQGHIPDWIGEIGNQLSILNLTNNSLTGTIPNLSDTFPGWLGGLPNIQALILKSNKFYGGIVTSTKDKFPFQRLQVLDISQNGFVGQLPTKYFQNFNAMKNVVKKNTKPEYVSTVYTYVSIILAVKGVDLYFSKLLVDYTIVDLSNNKFEGEIPNNIGNLKSLIVLNLSHNNLTGQIPHSLAKISEIESLDLSWNQLIGEIPQSLADLTSLEFLNLSQNRLVGRIPQGKQFNTFNGYSFGGNPKLCGLPLPKKCGEHLQKPQLEGDGDGEEEIGFTWKEVVMGYWCGALLGIVLGYLMLSTEKPKWFNAFVDETEHLILKRQNKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CAF2</accession>
    <name>A0AAD5CAF2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_002898</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0496 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008942">
        <property type="protein sequence ID" value="KAI7737835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CAF2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007749">
        <property type="entry name" value="DUF677"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31113:SF3">
        <property type="entry name" value="UPF0496 PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31113">
        <property type="entry name" value="UPF0496 PROTEIN 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05055">
        <property type="entry name" value="DUF677"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="binding site" evidence="6">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7737835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="393" mass="45855" checksum="7FEA8A7E958BCE3D" modified="2024-05-29" version="1" fragment="single">MSVLKDSKHLKIPLKEIEIATKNFQECIGKGGYGMVYKGELLVDGKSTTVAVKRLNEQFGQGLKEFLMEIQLLTGERPSMDLVTKELEELLQIQLSSYALESPRENSFSHEASYGSVFKMQGFHKTLQIRADNVINNLDTGIEDRSLSFDIEKEVNLFVMEMNQDERKVALNLKLVKLVDDYQEINTQTSGLYDSLSTCLRRVRGTRLLILEALRHVDDEDHGNEENRYERALDHLKSFKAAGDPFTQDFFRIFNFVYRRQIAFLEKLQRKNVKLSKKLDQTWLRKRFSSVLKHYKNNDIKWQKEVISFMQVVSYIEIKDTGRIQFLVNKLQMDIENFMRNAEFAIKMKDAVKYVIENMTEKLNTFLKNVDELEEMANICTVNIQKAKTVNRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CBE0</accession>
    <name>A0AAD5CBE0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein root UVB sensitive 1, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_018603</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RUS1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008730">
        <property type="protein sequence ID" value="KAI7738752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CBE0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009941">
        <property type="term" value="C:chloroplast envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032502">
        <property type="term" value="P:developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010224">
        <property type="term" value="P:response to UV-B"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006968">
        <property type="entry name" value="RUS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055412">
        <property type="entry name" value="UVB_sens_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054549">
        <property type="entry name" value="UVB_sens_RUS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12770:SF22">
        <property type="entry name" value="PROTEIN ROOT UVB SENSITIVE 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12770">
        <property type="entry name" value="RUS1 FAMILY PROTEIN C16ORF58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24160">
        <property type="entry name" value="UVB_sens_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04884">
        <property type="entry name" value="UVB_sens_prot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein root UVB sensitive/RUS" evidence="2">
        <location>
            <begin position="106"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="Root UVB sensitive protein C-terminal" evidence="3">
        <location>
            <begin position="388"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7738752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="514" mass="56741" checksum="0C68931E4A31487F" modified="2024-05-29" version="1" fragment="single">NNNNNNNNNNNNKKHVLYCLILALIIQFAYRAYAIDTGNEIGTNNDDEVIVWEVKGGKRTKLVGDNVLDMFVVPSVRWAWWDLQSGTKEDSSTTVSLGNVVPTMWVQCRELFMRLMLPEGYPESVTSDYMEYSCWRGVQGVAAQVSGVLATQSLLYAVGLGKGAIPTAAAVNWVLKDGIGYLSKIFLSKFGRHFDVNPKGWRLFADFMENAAFGMEILTPAFPHLFVPIGAAAGAGRSAATLIQAATRSCFFAGFAAQRNFAEVIAKGEAQGMVSKSIGIALGIALANCVQASTPLAMASFGVITWIHMFCNVKSYQSIQLRTLNPYRASLVFGEYLLSGLVPSVKEVNDEEPFFPALPLLSLKPASKVEHSLLSPEAKEAAAIIDQRLELGSKLSEIVKTKTDAHALLDLYKNQGYILTEQNGKFCVILKDTCSPQDMLKSMFHVSYLYWLEKNVGLKPVSIYDDCRPGGMLQISLEYVEREFNHAKHDGELAGWVTDSLVARPLPHRIRPDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CEC7</accession>
    <name>A0AAD5CEC7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Aminotransferase class I/classII large domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_009414</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008454">
        <property type="protein sequence ID" value="KAI7740341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CEC7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016847">
        <property type="term" value="F:1-aminocyclopropane-1-carboxylate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050478">
        <property type="entry name" value="Ethylene_sulfur-biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43795:SF39">
        <property type="entry name" value="AMINOTRANSFERASE CLASS I_CLASSII DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43795">
        <property type="entry name" value="BIFUNCTIONAL ASPARTATE AMINOTRANSFERASE AND GLUTAMATE/ASPARTATE-PREPHENATE AMINOTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00753">
        <property type="entry name" value="ACCSYNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="3">
        <location>
            <begin position="2"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7740341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="338" mass="38085" checksum="3059E2ACC4111E71" modified="2024-05-29" version="1">MASFMEQVRGGKAKFNPDRVVLTAGATAANELLTFILANPGDALLVPTPYYPGFDRDLRWRTGVQIVPIHCDSSNGFQITPEALEAAYDHATKMNIKVKGVLITNPSNPLGATIQRKVLEEILEFVTRKNIHLVSDEIYSGSAFHADEFVSIAEVLESRNYKDSERCHIVYSLSKDLGLPGFRVGTVYSYNDQVVTTARRMSSFTLISSQTQFLLASMLSDTKFTQKYIKINRERLKKRYEMIVNGLKKAGIECLKGKAGLFCWMNLSPYLEEPTIKGELAIWNTIMHEVRLNISPGSSCHCSEPGWFRVCFANMSEETLEVALSRIHEFIKERKQVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CH00</accession>
    <name>A0AAD5CH00_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Transmembrane 9 superfamily member</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_019446</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the nonaspanin (TM9SF) (TC 9.A.2) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008213">
        <property type="protein sequence ID" value="KAI7741329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CH00"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072657">
        <property type="term" value="P:protein localization to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004240">
        <property type="entry name" value="EMP70"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10766:SF180">
        <property type="entry name" value="TRANSMEMBRANE 9 SUPERFAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10766">
        <property type="entry name" value="TRANSMEMBRANE 9 SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02990">
        <property type="entry name" value="EMP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="84"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="119"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="154"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="185"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="245"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="280"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7741329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="321" mass="36438" checksum="89BEFC65AB40E185" modified="2024-05-29" version="1" fragment="single">MEKYTNYSSVPQDNNIRGYSIANSSLTILIFIICLLIFYIRVLRKDISRYACDVEEAEMAGNQEETGWKKIHGDVFRYPKHKSLFAAALGSGTQLLVLTVAILVLGVTDPFHLHRPGVFVYALAIVYALTSASSGYTSTSFYQQLEGRRWMKNVLLTGGLYFAPLFLTFIVNNTVAFFYEITTTLPLGAIINLSLLWVFLALPLLILGAVIGKNSVLDFEAPCRTAKCPKEVPRLRWYRRVLPQMVLAGFLSFGVILIHTIEILNAVWRYKIYTSYGSMLIMLFLLLIMTALVSIVMTYFQLAAENHEWWWRSFFCGGSTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CHL6</accession>
    <name>A0AAD5CHL6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ATP-dependent zinc metalloprotease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_017774</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008050">
        <property type="protein sequence ID" value="KAI7742218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CHL6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.760:FF:000007">
        <property type="entry name" value="ATP-dependent zinc metalloprotease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.760">
        <property type="entry name" value="Peptidase M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037219">
        <property type="entry name" value="Peptidase_M41-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33471">
        <property type="entry name" value="ATP-DEPENDENT ZINC METALLOPROTEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33471:SF7">
        <property type="entry name" value="ATP-DEPENDENT ZINC METALLOPROTEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140990">
        <property type="entry name" value="FtsH protease domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7742218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="367" mass="40725" checksum="250B79731FC8B79F" modified="2024-05-29" version="1">MAIYLRCIPPSPFLPSRPLQLRQLSRNGMTPKAITEWKEYEQAVKDKDLGKALRFLQESPQLDDYYSETDNGLLDLKRDWDVLDTCLNADDMRLVASAYSFLKDKGFLNYFGKYRSIVLEGPRDVTPTVLKSSTGLEVSKLSPKKFGLSTSSSVVLVAFFAGLSYLVDHGVDLRPQLAAVLGLSMVDSIFLGGTCLAQISSYWPPYKRRILVHEAGHLLVAYLMGCPIRGVILDPIVAMQMGIQGQAGTQFWDENLQNELAEGRLTGVAFDRYCMVLFGGIAAEALVYGEAEGGENDENLFRSICLLLEPPFSVAQMSNQARWSVLQSYNLLKWHKQAHRAAVKALENGGSLSVVIRSIEEAMAANR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CHV6</accession>
    <name>A0AAD5CHV6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Phosphoglycerate kinase</fullName>
            <ecNumber evidence="12">2.7.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_006318</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>(2R)-3-phosphoglycerate + ATP = (2R)-3-phospho-glyceroyl phosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:14801"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57604"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>guanosine(46) in tRNA + S-adenosyl-L-methionine = N(7)-methylguanosine(46) in tRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42708"/>
            <dbReference type="Rhea" id="RHEA-COMP:10188"/>
            <dbReference type="Rhea" id="RHEA-COMP:10189"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74480"/>
            <dbReference type="EC" id="2.1.1.33"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="13">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the phosphoglycerate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.3" evidence="12"/>
    <dbReference type="EMBL" id="JAMZMK010008270">
        <property type="protein sequence ID" value="KAI7741125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004618">
        <property type="term" value="F:phosphoglycerate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008176">
        <property type="term" value="F:tRNA (guanine(46)-N7)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000194">
        <property type="entry name" value="Phosphoglycerate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1260">
        <property type="entry name" value="Phosphoglycerate kinase, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001576">
        <property type="entry name" value="Phosphoglycerate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015824">
        <property type="entry name" value="Phosphoglycerate_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036043">
        <property type="entry name" value="Phosphoglycerate_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003358">
        <property type="entry name" value="tRNA_(Gua-N-7)_MeTrfase_Trmb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11406">
        <property type="entry name" value="PHOSPHOGLYCERATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11406:SF32">
        <property type="entry name" value="PHOSPHOGLYCERATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02390">
        <property type="entry name" value="Methyltransf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00162">
        <property type="entry name" value="PGK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00477">
        <property type="entry name" value="PHGLYCKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53748">
        <property type="entry name" value="Phosphoglycerate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51625">
        <property type="entry name" value="SAM_MT_TRMB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7741125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="695" mass="77994" checksum="A6EC2DB2FD06E8DC" modified="2024-05-29" version="1">MSRILDIHYCPPTYSFMFFTSKSQYFITNHVHFKKQQAVPSSLQAFSSEMGLDHLTERCSFSNKVKGEEGNVYDTLPHVRYLREFQKDELIGKVVMVRFDSNVLLGEKQDRQAKLFDSAVLTVKYLHEAGATVILVGSWSVKTYSNFRSADSVSAYLSSILELRVVPVKSFLGCKQPTMENSRTQSIFLLENLSKVKEDAANCSRFSEQLCAGVDIFVNDAFSQSHKVLGSTVGVTSFCYASVAGFQFEESLVQLKNAFGTKKNPYIAMVFYXXXXXXXXXXDILVQIGGGNLVEKAAAVHYLVSSADGLVFVGNMAFQIMHALGLPVPRRLAEVGAFTEANRIMKFANSRNIPILFPKDFWCRNDHLKKPELVPVHGILEGWSPIALGPNSLEEITTLLSKSKKVVWIGPVKFDSAIQDSSGTSILVKMLRKLSQRNCDVTVVGNTACKALMGESAVLSSCNIIENASVVWEFLKGRKLPGLVALDRGYPFSIDWHAIYADPTQPLLVDIGSGNGLFLFGMAKKRKDMNFLGLEMNGKLVKRCLDAVHQSGLKNGHFIETNATSTFRSIVSGYPGELVLASIQCPNPDFNKPEHRWKMVQRSLIEAIRDRLSSKGKVFLQSDIEAVALRMKQQFLKYGNGEFTIDHQEEWLRENPFGVQSDWEQHVLRRGVPMYRLMLSKCREEPKFKHYLHIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CI39</accession>
    <name>A0AAD5CI39_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_023855</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008234">
        <property type="protein sequence ID" value="KAI7741245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CI39"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7741245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="54" mass="5942" checksum="4EC4395E7846A15F" modified="2024-05-29" version="1">MNNNKEPRSVRKQGCGGFELCVGEIVALWWVRMVVRRETVVVEGDDGGGFGFGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CK69</accession>
    <name>A0AAD5CK69_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Outer envelope pore protein 16-3, chloroplastic/mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_018494</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007925">
        <property type="protein sequence ID" value="KAI7742710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CK69"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042721">
        <property type="term" value="C:TIM22 mitochondrial import inner membrane insertion complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045039">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039175">
        <property type="entry name" value="TIM22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14110">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14110:SF18">
        <property type="entry name" value="OUTER ENVELOPE PORE PROTEIN 16-3, CHLOROPLASTIC_MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02466">
        <property type="entry name" value="Tim17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="61"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="91"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7742710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="159" mass="16725" checksum="457BEA70153D84E6" modified="2024-05-29" version="1">MDATDRRYFEEEDSSLTKTMKGAATGLAAGTIWGTIVATWLDVPRVERNVALPGLIRTLKMMGNHGLTFAAIGGVYIGVEQLTQNYRQKRDFVNGAVGGFVAGAAILGFKGKSISTAISAGAALAATSAAIDVGGQTTRVDTGKEYYPYTTKKRADAAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CK92</accession>
    <name>A0AAD5CK92_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cycloidea-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_007827</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007711">
        <property type="protein sequence ID" value="KAI7743613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CK92"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000032">
        <property type="term" value="P:regulation of secondary shoot formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017888">
        <property type="entry name" value="CYC/TB1_R_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017887">
        <property type="entry name" value="TF_TCP_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005333">
        <property type="entry name" value="Transcription_factor_TCP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31072:SF224">
        <property type="entry name" value="TRANSCRIPTION FACTOR TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31072">
        <property type="entry name" value="TRANSCRIPTION FACTOR TCP4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03634">
        <property type="entry name" value="TCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51370">
        <property type="entry name" value="R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51369">
        <property type="entry name" value="TCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TCP" evidence="8">
        <location>
            <begin position="64"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="R" evidence="9">
        <location>
            <begin position="177"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="147"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="147"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7743613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="228" mass="26292" checksum="DF9963929A3ACF79" modified="2024-05-29" version="1" fragment="single">IMSSTNPPSNFFLEHEKDDVYNNSLVSSDCFPVKENITTRKQDLGLEECDENENVCWSVVKTSNKDHHSKIQTAQGPRDRRVRFSIEVAKRFFYLQELLGFDKGSKTLDWLLKKSKNSIDELIKRKKESSSSSSFTDQSEVVFLETGSEKAKARKKKGDEEKTKKKTRRYKSLVNVNQSRVEARARARERTKEKLNVKKLDEESKNIHMFMSIGLVTMQPFRSFGGPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CLG7</accession>
    <name>A0AAD5CLG7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_030356</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007584">
        <property type="protein sequence ID" value="KAI7744106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CLG7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12325">
        <property type="entry name" value="RRM1_hnRNPA_hnRNPD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12330">
        <property type="entry name" value="RRM2_Hrp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000051">
        <property type="entry name" value="Heterogeneous nuclear ribonucleoprotein 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000102">
        <property type="entry name" value="Heterogeneous nuclear ribonucleoprotein 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48032:SF16">
        <property type="entry name" value="RNA-BINDING (RRM_RBD_RNP MOTIFS) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48032">
        <property type="entry name" value="RNA-BINDING PROTEIN MUSASHI HOMOLOG RBP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="6"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="106"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="493"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="593"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="425"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="568"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="892"/>
            <end position="962"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="439"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="574"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="892"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="924"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="962"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7744106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="962" mass="100837" checksum="D289FB338C775BA6" modified="2024-05-29" version="1" fragment="single">MQSDLGKLFIGGIAWDTNEERLHEYFSSFGEVLEAVIMKDHTTGRARGFGFVVFADPAVAERVIKEKHHIDGRMVEAKKAVPREDQSIVSRNSGSIQSSPGLNRTRKIFVGGLASTVTESDFRRYFEQFGTITDVVVMYDHNTQRPRGFGFITYDSEDAVDTVLLKTFHELNGKMVEVKRAVPKELSPSPSRTMLGVYPYGMGRASGILNGYAQGPYSPGAVGGYGVRMAGRGGFGPFGSGYGMGLNFEPGMTSPSYNRSLSYGRGMSPSPYFVGNTNRFAGPVGFDGGNGGGNGGNASFFSSNPRNLWGNTGVNYGSNSTGSSAIGGGIFGNAGLSWGSNPITNQGSGNASSQSGNLGYGGENGYGIGVGAYGRNVTTSEVQTSSYAAANGGYEGPFSDFYVGGSGSGGGGGGGGVSNSLYGVDPSWRSGNSEQDGSGSFGGYGVGGGGSDVHTRSPPGYVDGYSVAKRQTNRGGSQLCFFIESEKMQSDLGKLFIGGISWDTNEERLQEYFSSFGEVLEAVIMKDHTTGRARGFGFVVFADPAVAERVIKEKHHIDGRMVEAKKAVPREEQSTISRNSGSLQSSPGLNRTRKIFVGGLASTVTENDFRRYFEQFGTITDVVVMYDHNTQRPRGFGFITYDSEDAVDKVLLKTFHELNGKMVEVKRAVPKELSPSPSRTMLGVYPYGMGRTSGILNSYTQGPYSPGAVGGYGVRMAGRGGFDPFGSAYGMGLNFEPGMTNPNYNRSLSYGRGMSPSPYFVGNTNRFAGPVGFDGGNGGGNGGNASFFSSNPRNLWGNSGFNYGSNTTRSSAIGGGIFGNAGVNWGSNPVTNQVGRNASTQSGNLGFGGDNGYGLGVGEYGRNVTTSQVRTSPYAAANGGYEEPFSDFYAGGSGGGGGGGGGGSSVYGGDPTWRSRNSEQEGSGSFGGYGIGGSSDGGGSDVHTKSPGYGDGYSVAKRQTNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CMV9</accession>
    <name>A0AAD5CMV9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">dolichyl-P-Man:Man5GlcNAc2-PP-dolichol alpha-1,3-mannosyltransferase</fullName>
            <ecNumber evidence="3">2.4.1.258</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_001801</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>an alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate = an alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:29527"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="Rhea" id="RHEA-COMP:19516"/>
            <dbReference type="Rhea" id="RHEA-COMP:19517"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:132515"/>
            <dbReference type="ChEBI" id="CHEBI:132516"/>
            <dbReference type="EC" id="2.4.1.258"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:29528"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.258" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010007609">
        <property type="protein sequence ID" value="KAI7744015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CMV9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052925">
        <property type="term" value="F:dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007873">
        <property type="entry name" value="Glycosyltransferase_ALG3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12646:SF0">
        <property type="entry name" value="DOL-P-MAN:MAN(5)GLCNAC(2)-PP-DOL ALPHA-1,3-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12646">
        <property type="entry name" value="NOT56 - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05208">
        <property type="entry name" value="ALG3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="110"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="151"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="180"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="211"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="272"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="402"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7744015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="440" mass="50408" checksum="9A98A42270291B9A" modified="2024-05-29" version="1">MAISAARNRKPPKRRSDNIIQKIYKNPKTPFAFSLLLADALLTALIIAYVPYTKIDWDAYMSQVDGFIEGERDYSNLKGDTGPLVYPAGFLYIYSAIQYVTGGEVYPAQILFGFLYIVNLGIVLFIYVKTDVLPWWALTLLSLSKRLHSIFVLRLFNDCFAMTLFHASLVPLLYHKWHLSLIIFSAAVSVKMNVLLYAPPLLLLMVKAMDFIGVISALTGAALVQILLGLPFLWSYPIEYLSKAFNLGRVFIHFWSVNFKFVPEPVFVSKEFALVLLVIHLGLLLIFVQYRWCKHEGGLVSFLRSRFVQMRLKTSITSSFPLKKSHRSSSGTKILKNEHIVTTMFTGNFIGIVCARSLHYQFYSWYFYSLPYLLWRTPFPTVVRLLLFAGVEFCWNVFPSTVYSSLVLLCVHIIILWGLWRAPPEYPYENDRSQAGMKAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CPA1</accession>
    <name>A0AAD5CPA1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_017225</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007300">
        <property type="protein sequence ID" value="KAI7745304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CPA1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009809">
        <property type="term" value="P:lignin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05283">
        <property type="entry name" value="CAD1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000022">
        <property type="entry name" value="Cinnamyl alcohol dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.180.10:FF:000004">
        <property type="entry name" value="probable cinnamyl alcohol dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.180.10:FF:000100">
        <property type="entry name" value="Putative cinnamyl alcohol dehydrogenase 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047109">
        <property type="entry name" value="CAD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029752">
        <property type="entry name" value="D-isomer_DH_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42683">
        <property type="entry name" value="ALDEHYDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00065">
        <property type="entry name" value="D_2_HYDROXYACID_DH_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="7">
        <location>
            <begin position="23"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7745304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="673" mass="72354" checksum="1456B985752E05CC" modified="2024-05-29" version="1">MATSPESEHPIKAYGYAARDTSGSLSPLTFSRRATGEKDVRFKVLYCGICHSDLHFAKNEWGITKYPVTPGHEIVGVVTEVGSKVEKFKIGDKVGVGCLVGSCRSCQGCATDYEQYCPKQVATYGMRNFDGTQTYGGYSDHMVSDEHFVLRWPVNLPLDSGAPLLCAGITTYSPLRYFGLDKPGTKVGVVGLGGLGHVAVKMAKAFGAEVTVFSTTPAKEQEAIEGLKADHFINSKDERQMQAARGTLDGIIDTVSGTHPIAPLLNALTPHGKLVLVGLPEKPLDVSAFSLIMGRKTLAGSSIGGIKETQEMLDFAAKHGITADIEVIPIDYVNTAMERLLKSDVRATGEKDVRFKVLYCGICHSDLHFVKNEWGITKYPVTPGHEIVGVVTEVGSKVEKFKIGDKVGVGCMVGSCRSCQSCASDYEQYCAKQVSTYGGRSFDGTQTYGGYSDHMVSDEHFVLRWPDNLPLDSGAPLLCAGITTYSPLKYFGLDKPGTKVGVVGLGGLGHVAVKMAKAFGAEVTVFSTTPAKEQEAIEGLKADHFINSKDENQMQAARGTLDGIIDTVSANHPIVPLLNTLTPHGKLVLVGAPEKPLEVAAFSLIMGRKLVAGSNIGGIKETQEMLDFAAKHGITADIEVIPIDYVNTAMERLLKSDVRYRFVIDVANSIKAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CRR7</accession>
    <name>A0AAD5CRR7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_006307</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006866">
        <property type="protein sequence ID" value="KAI7746963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CRR7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039923">
        <property type="entry name" value="Protodermal_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33210:SF24">
        <property type="entry name" value="POLLEN OLE E 1 ALLERGEN AND EXTENSIN FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33210">
        <property type="entry name" value="PROTODERMAL FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01190">
        <property type="entry name" value="Pollen_Ole_e_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042117159" evidence="2">
        <location>
            <begin position="28"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="141"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="141"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="151"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7746963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="315" mass="33983" checksum="6168269A466A7A2C" modified="2024-05-29" version="1">MGVVTTMGVCLLTTIVILGAVIGGANGDAMVSGSVFCDRCKDGQLSLFDFPLAGIKVSIACPGQDGQMKVISEETTNWIGNYVMRFDGAPDMSACRAQVFGDGQGCRVVSGPTQSLNLVFQMFDTEIYTVNHLLSQPAQPMSNCPLSSSPLPKPVTPTVPPPTNRAPAPQPPKLPPQPRLPPMPPVPFLEASACSYRMWMMPEHECYWRVLSPDLKVAFVFGPVAGQKYGNDITLRGSMSGRGDPYKTLLRESTTALLNSYNSIQFPYHPLDVVGHFNSALMGGSTRQVLMTALRFLRANSGNPGNVTCKFTTCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CSQ2</accession>
    <name>A0AAD5CSQ2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_024611</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006717">
        <property type="protein sequence ID" value="KAI7747616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CSQ2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7747616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="117" mass="12674" checksum="E39AC1DC1F25C9DA" modified="2024-05-29" version="1" fragment="single">SNDSSLRQPQISPDAQKKTKIVCTIGPSTSSQYNAQFNEKVKAIMLDTKPLCDALGVGTWKFAKSLRRMEELISNEGGLSDGVDNLDQGDQEGNPSQAKIECVVNDQEGEASQAKDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CWG0</accession>
    <name>A0AAD5CWG0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Vesicle-associated membrane protein 726</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_012405</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in the targeting and/or fusion of transport vesicles to their target membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Endomembrane system</location>
            <topology evidence="8">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the synaptobrevin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006496">
        <property type="protein sequence ID" value="KAI7748605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CWG0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14824">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15843">
        <property type="entry name" value="R-SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000109">
        <property type="entry name" value="Vesicle-associated membrane protein 722"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.50:FF:000002">
        <property type="entry name" value="Vesicle-associated membrane protein 722"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.50">
        <property type="entry name" value="Longin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010908">
        <property type="entry name" value="Longin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001388">
        <property type="entry name" value="Synaptobrevin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051097">
        <property type="entry name" value="Synaptobrevin-like_transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042855">
        <property type="entry name" value="V_SNARE_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21136:SF203">
        <property type="entry name" value="LONGIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21136">
        <property type="entry name" value="SNARE PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13774">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00957">
        <property type="entry name" value="Synaptobrevin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00219">
        <property type="entry name" value="SYNAPTOBREVN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01270">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50859">
        <property type="entry name" value="LONGIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00417">
        <property type="entry name" value="SYNAPTOBREVIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50892">
        <property type="entry name" value="V_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Longin" evidence="10">
        <location>
            <begin position="10"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="V-SNARE coiled-coil homology" evidence="11">
        <location>
            <begin position="130"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7748605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="181" mass="20364" checksum="D9F4BF0E014AE96F" modified="2024-05-29" version="1">MGQQSLIYSFVSRGTVILAEYTEFTGNFTSIASQCLQKLPTTNNKFTYNCDGHTFNYLVEDGFTYCVVAAESAGRQVPMAFLERIKDDFNKKYGGGKAATAIANSLNKEFAPKLKEQMQYCVDHPEEISKLSKVKAQVSEVKGVMMENIEKVLDRGEKIELLVDKTENLRSQVREFKFLSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CWT7</accession>
    <name>A0AAD5CWT7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA (cytosine-5-)-methyltransferase</fullName>
            <ecNumber evidence="2">2.1.1.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_011767</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a 2'-deoxycytidine in DNA + S-adenosyl-L-methionine = a 5-methyl-2'-deoxycytidine in DNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13681"/>
            <dbReference type="Rhea" id="RHEA-COMP:11369"/>
            <dbReference type="Rhea" id="RHEA-COMP:11370"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:85452"/>
            <dbReference type="ChEBI" id="CHEBI:85454"/>
            <dbReference type="EC" id="2.1.1.37"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.37" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010006272">
        <property type="protein sequence ID" value="KAI7749813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CWT7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003886">
        <property type="term" value="F:DNA (cytosine-5-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044027">
        <property type="term" value="P:negative regulation of gene expression via chromosomal CpG island methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18635">
        <property type="entry name" value="CD_CMT3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.120.10">
        <property type="entry name" value="DNA Methylase, subunit A, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001025">
        <property type="entry name" value="BAH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043151">
        <property type="entry name" value="BAH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050390">
        <property type="entry name" value="C5-Methyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001525">
        <property type="entry name" value="C5_MeTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031303">
        <property type="entry name" value="C5_meth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023779">
        <property type="entry name" value="Chromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10629">
        <property type="entry name" value="CYTOSINE-SPECIFIC METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10629:SF44">
        <property type="entry name" value="DNA (CYTOSINE-5-)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01426">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00145">
        <property type="entry name" value="DNA_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00439">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51038">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00095">
        <property type="entry name" value="C5_MTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00598">
        <property type="entry name" value="CHROMO_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51679">
        <property type="entry name" value="SAM_MT_C5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="BAH" evidence="12">
        <location>
            <begin position="152"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="Chromo" evidence="11">
        <location>
            <begin position="417"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="17"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="28"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="49"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="495"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7749813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="886" mass="101028" checksum="7441CAF804201422" modified="2024-05-29" version="1">MVADNVDGADGFTFRPPSNPSLSPPPSITATQEGQFGQISRRQSPRFYNCKNNNNNNNNKDSSDDHNKPPMKKKHKVASASESFALSSNDSSFFIGDPVPDEEARRRWPWRYDCMGLKSDGRGHKTKNNDDDDDMLVLNVKCHYLQAKVETHVFELGDCATVKGEGDHDHVGRILEFFKTNDNQDFFRVQWFFRAEDTVIKDASSSNDKRRLFYSTLMNDNALDCIISKVKVVKIQPGANLKSIPTCDYYYDMKYNIDYSTFSTIKDEVSSNNWCLFSCDNKEEINSNGSIDNKSNENITNFEPKKLELTVLDMYSGCGGMSTGLHFGAKLSGVDLSTKWAVDANESACESLKLNHPETQIRNESASDFLDLIKEWDKLCKKYPVKKERQRHDSMVDGPPDDVIPSKKHNIVPKDEYEVEKLVDICYSELDGTCKRGLKFKVRWAGYSPSDDTWEPIEELSKCKDKICEFVQKGIKANILPRPADVGLICGGPPCQGISGYNRHRNLKSPLEDEKNYQIVNLPPFPLPTHDVVFKHGGANGFERNIVAYDEGKHPKLEKCVLLKDAISDLPPVLNSEIRDKMKYKSPPETEFQKFIRATKSDMLGIASGSLRKAQKPLLHDHRPLPLNEDDYLRVCRVPKRKGANFRDLPGVVFDDDNLVSRASEPELMPSGKHWVPDYALNFLHKKNTRHEILSTFCFYFYKILFFFSPFMFFNEMSDLLHAYGGMRRFQQYFVHQIFVLRVLTIRENARLQGFPDFYALSGSVKERYRQVGNAVAIPVGRVLGYTLGMALQKNISKIVLDVVENSMDKADKNAVKMKLRKPWKIRGWIVKIIEANEVNPIVADEILSIDVRDLALTEKLGAVEKQDNLEQECNENDVAIKRYYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CXZ0</accession>
    <name>A0AAD5CXZ0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">SP-RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_013410</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein sumoylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NSE2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006158">
        <property type="protein sequence ID" value="KAI7750393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CXZ0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030915">
        <property type="term" value="C:Smc5-Smc6 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061665">
        <property type="term" value="F:SUMO ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16651">
        <property type="entry name" value="SPL-RING_NSE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026846">
        <property type="entry name" value="Nse2(Mms21)"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004181">
        <property type="entry name" value="Znf_MIZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21330">
        <property type="entry name" value="E3 SUMO-PROTEIN LIGASE NSE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21330:SF1">
        <property type="entry name" value="E3 SUMO-PROTEIN LIGASE NSE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11789">
        <property type="entry name" value="zf-Nse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51044">
        <property type="entry name" value="ZF_SP_RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SP-RING-type" evidence="12">
        <location>
            <begin position="99"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="188"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="197"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7750393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="211" mass="23762" checksum="14B765766D19B456" modified="2024-05-29" version="1" fragment="single">DIRKAMTTMKEIGVKELETEFMELLKAYEECANLSTAIDSVGNMYRPTEQLTDFKKLIDNEMAKTTRRSSAPQVAQLLRQFKEAIWNVHNSGLPMPGEEQEDIIVTSTQSILLNNTCPLSGKPVTELSEPVRSMDCNHIYEKSVIMQYIRSKHGKPKCPVAACPKIITAERVVCDPLLHVEIEESRAMNKRTSNPDIIEDFTGDSENEGSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CY24</accession>
    <name>A0AAD5CY24_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_028922</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006150">
        <property type="protein sequence ID" value="KAI7750451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CY24"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7750451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="113" mass="12744" checksum="2D977EE1BCE4B692" modified="2024-05-29" version="1" fragment="single">VCNLEASLYQKILHIILHHRHTRDVRLHLFTATAASPSDLIPLSGDLCYNDRRCQSTSAGGGCVVSGSDRWRTCLVSHPPATLLRMVRTHFILSFWDVIGKQFHLESMFLKNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CZ66</accession>
    <name>A0AAD5CZ66_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_013196</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005988">
        <property type="protein sequence ID" value="KAI7751081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CZ66"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33400:SF2">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33400">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="4">
        <location>
            <begin position="297"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="2">
        <location>
            <begin position="297"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="329"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="340"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7751081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="354" mass="39142" checksum="721F4C9441E3F1D4" modified="2024-05-29" version="1">MLGKRGSELRIGCSRTYIHNIRQIDWACPPRFLVDPDWKMEAGSESQETHIQLQRESRIADVIYYHKSDIPPNPYVAPNAFDLNDDSGIPVIPLEVINDETSPNDPLIPPEPTVTSTRPLLPSDEVIGTAVALVALIKSKEPGSQIDLELLIQLLCNPGILEKLTKEHGLSANQSSVITRAQPRECSSVPLPVPMPAVGGYSTWFTSTPFSRSLPQGVRNMIEQIHSNSESQNPSSNRKVDVSKLRKLVNEYGLGDDLVKRGPAVVKDADYYRRLISQHGMSQENDKPQESVKLTASQEQRPCVYFNSPKGCRRGSGCRFQHVKGGFGGGGQAVEEQHGAKRMKLSGRNFGRHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CZI6</accession>
    <name>A0AAD5CZI6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_024879</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006253">
        <property type="protein sequence ID" value="KAI7749929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CZI6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000001">
        <property type="entry name" value="Calmodulin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039647">
        <property type="entry name" value="EF_hand_pair_protein_CML-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10891">
        <property type="entry name" value="EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="42"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="80"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="123"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="160"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7749929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="194" mass="21460" checksum="4D2CB6CF220870C1" modified="2024-05-29" version="1">MPSSLQTHIKNFLKKLSLLTKLNKSSKKLCALDVISSFIAMDVSNQLKQVFKFFDNDGDGKISQMELTNVLLTFGHEKSKATIEAQGILKEVDFNGDGFIDLDEFMAIMDGSKPVFACSKEDDGDDDLRNAFLVFDSDKNGLISAMELQRVLISLGCSNSKLGKCRKMIKGVDTNGDGFVDFEEFKSMMSIGIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D0W6</accession>
    <name>A0AAD5D0W6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Selenoprotein O</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_001571</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SELO family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005788">
        <property type="protein sequence ID" value="KAI7751928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D0W6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009534">
        <property type="term" value="C:chloroplast thylakoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070733">
        <property type="term" value="F:AMPylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00692">
        <property type="entry name" value="SelO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003846">
        <property type="entry name" value="SelO"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000658">
        <property type="entry name" value="PRK00029.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32057">
        <property type="entry name" value="PROTEIN ADENYLYLTRANSFERASE SELO, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32057:SF14">
        <property type="entry name" value="PROTEIN ADENYLYLTRANSFERASE SELO, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02696">
        <property type="entry name" value="SelO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="9"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7751928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="578" mass="64468" checksum="D9A064D23D5DFF31" modified="2024-05-29" version="1">MSTEPPPTAAVAPPLDPVAGEPDNKTSTENPNVKLKLEDLNWDHSFVRELPADPLTDISSREVLHACYSRVSPSAEVENPQLVVWSESVADILDLDPKEFERPDFPYLFSGASPLKGGLSYAQCYGGHQFGVWADQLGDGRAITLGELINSKSQRWELQLKGAGKTPYSRNADGLAVLRSSIREFLCSEAMHNLGIPTTRALCLVTTGKLVSRDMFYDGNPKDEPGAIVCRVAQSFLRFGSFQIHAKRGNEDLEIVKTLADYTIKHHFPHLESMSKSDSLSFSTGEGNSDVVDLTSNKYAAWLVEVAERTASLIAKWQGVGFTHGVLNTDNMSVLGLTIDYGPFGFLDAFDPSFTPNTTDLPGRRYCFAEQPDIGSWNIGQFGLALSTAQLINKKELNYALERYGSKFMDEYQAIMTKKVGLSRYNKELIGELLNNMAVDKVDYTNFFRLLSNIKADSDVPDKDLLVPLKAALLDIGRERKEAWTGWVKRYRAELLSSGVSDEERKVSMNSVNPRYILRNYLCQSAIDMAEQGDYEEVRRLLKVMEKPFDDQPGMERYARLPPAWAYRPGVCMLSCSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D0X2</accession>
    <name>A0AAD5D0X2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_031129</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005996">
        <property type="protein sequence ID" value="KAI7751072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D0X2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024097">
        <property type="entry name" value="bHLH_ZIP_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12565">
        <property type="entry name" value="STEROL REGULATORY ELEMENT-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12565:SF312">
        <property type="entry name" value="TRANSCRIPTION FACTOR BHLH74"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7751072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="127" mass="13877" checksum="D62F15FF8B91D29E" modified="2024-05-29" version="1" fragment="single">ITGKAVMLDEIINYVQSLQQQVEFLSMKLATVNPEVNVDIDRLVFKDMLHSRAGTSNPAYGFGPTLSPSHSYPHGSLPGIPTTAATMHPIHTQPVWDNDLHNLLQMGFDANPGINNLGPNGRSKMDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D4K3</accession>
    <name>A0AAD5D4K3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">monogalactosyldiacylglycerol synthase</fullName>
            <ecNumber evidence="2">2.4.1.46</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_026726</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Plastid</location>
            <location evidence="5">Chloroplast membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 28 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.46" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010005567">
        <property type="protein sequence ID" value="KAI7752985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D4K3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031969">
        <property type="term" value="C:chloroplast membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046509">
        <property type="term" value="F:1,2-diacylglycerol 3-beta-galactosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009247">
        <property type="term" value="P:glycolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17507">
        <property type="entry name" value="GT28_Beta-DGS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009695">
        <property type="entry name" value="Diacylglyc_glucosyltr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007235">
        <property type="entry name" value="Glyco_trans_28_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050519">
        <property type="entry name" value="Glycosyltransf_28_UgtP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43025">
        <property type="entry name" value="MONOGALACTOSYLDIACYLGLYCEROL SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43025:SF3">
        <property type="entry name" value="MONOGALACTOSYLDIACYLGLYCEROL SYNTHASE 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04101">
        <property type="entry name" value="Glyco_tran_28_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06925">
        <property type="entry name" value="MGDG_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Diacylglycerol glucosyltransferase N-terminal" evidence="7">
        <location>
            <begin position="144"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 28 C-terminal" evidence="6">
        <location>
            <begin position="365"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00046299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF06925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7752985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="522" mass="57369" checksum="DF18C85782F739EE" modified="2024-05-29" version="1" fragment="single">ALETNNAFKILSKLSNFAFELNSNKLSLDGYSSVLSNVNSDRKYVNFWAGGTSKSSKIVGSLSSGGKGGSSVSKFFNEFNKFIRFHCDKIPLGFASVKVNSGSECRLGGENEGVLEDGTLPLSGYESNGKKKVLILMSDTGGGHRASAEAIKWAFNEKFGDDYEVFVTDLWTDHTPWPFNQLPRSYNFLVKHGSLWRMTYYASAPRLVHQSNFAATSTFIAREVAKGLMKYQPDIIISVHPLMQHVPLRILRSKGLLDKIVFTTVVTDLSTCHPTWFHKLVTRCYCPTEEVAKRALKAGLQSSQIKVYGLPVRPSFVKPLRPKIELRRELGMDEGLPAVLLMGGGEGMGPIEATAKALGNALYDENLGEPIGQVLVICGRNKKLANKLLSAEWKIPIQVKGFVTNMEEYMGACDCIITKAGPGTIAEAMICGLPIILNDYIAGQEVGNVPYVVENGCGKFSKSPKEIANIVGEWFGPKAHELQAMSQNALRLARPDSVFKIVQDLHELVKQRSLIMQYSAAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D4S6</accession>
    <name>A0AAD5D4S6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_033117</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005069">
        <property type="protein sequence ID" value="KAI7753918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D4S6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7753918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="27" mass="3215" checksum="A120B2BFB9BC7764" modified="2024-05-29" version="1">MALMALKRLYSLKLKAVVWRCICMELM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D712</accession>
    <name>A0AAD5D712_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_023540</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010001854">
        <property type="protein sequence ID" value="KAI7755058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D712"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11073">
        <property type="entry name" value="CYP76-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000207">
        <property type="entry name" value="Putative cytochrome P450 superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47951:SF7">
        <property type="entry name" value="FLAVONOID 3',5'-HYDROXYLASE-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47951">
        <property type="entry name" value="OS08G0547900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0349">Heme</keyword>
    <keyword evidence="2 3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="1 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="23"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="2">
        <location>
            <position position="465"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7755058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="514" mass="58788" checksum="22F35D22E549A931" modified="2024-05-29" version="1">MAQKNNHGSWCWEVMSSSNKDEFTLPILVTIAAVMLAISWYKFTQSSTKPPLPPGPRSIPIVGYLPFLGHDLHKQFTNMAHTYGPVFKLHLGSKLYIVINNIELAKAVVRDQDEIFANRDLSIAASVISYGRQDLVWSMNNSNWRNLRKIFVHEALSNKNLEACSSFRRDEVRNTIKKVYSKIGTPIDISDVTFMTQTNVITSLIYGNTSDNHLGSELQMVAWNIVDIIGRPNLADFFPILARFDLQRVERDMKKLQRLDRIIETMIENRIKSNTEKSHEGVIDHERKKDFLQILLDLRDQEDVNSLSITNMKALLQDIMVAGTDTTTKVIEWVIAEMLKNSHVMKKAQEELADVVGLNNIVEETHLAKLKYLDAVIKETLRLHPVVPFLIPRSPSQDCVVGGYTIPKGSTVFLNVWSIQRDPRYWDNPLEFNPERFLTYEGNKKCDYKGNNLKFIPFGSGRRLCAGLPLAEKMMMFTLASLLHSFNWRLPEGEELDLSEEFGITLKKCIINIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D7C5</accession>
    <name>A0AAD5D7C5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Protein PIN-LIKES 7-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_032753</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in cellular auxin homeostasis by regulating auxin metabolism. Regulates intracellular auxin accumulation at the endoplasmic reticulum and thus auxin availability for nuclear auxin signaling.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the auxin efflux carrier (TC 2.A.69.2) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000835">
        <property type="protein sequence ID" value="KAI7755746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D7C5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009734">
        <property type="term" value="P:auxin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080162">
        <property type="term" value="P:endoplasmic reticulum to cytosol auxin transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004776">
        <property type="entry name" value="Mem_transp_PIN-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045033">
        <property type="entry name" value="PILS1/3/4/5/7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31651">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31651:SF27">
        <property type="entry name" value="MEMBRANE TRANSPORT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03547">
        <property type="entry name" value="Mem_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0927">Auxin signaling pathway</keyword>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="32"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="73"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="251"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="285"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="316"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7755746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="335" mass="36317" checksum="E7B552DFAE420399" modified="2024-05-29" version="1" fragment="single">MPINIGVTFLCGGILGWVAVKLIKPKPHLEGLIIAMCSTGNYANLLLIMVPAICTDDGSPFGDHNVCKAKGLSYASFSMALGSFYTWTCTYQMIQGSALRYNALKETGKLSNEANKDLAANQNTRLLNKEYHDHIDQIMPLPNSTNDDTENQCIVYQGSAENEEKKVGSFSDQSVDMLKKILEQLFAPPTLGLVFGAIPWVKNLLVGEKAPLRVIQDSMTLLGDGTIPCLTLILGGNLTQGLRKASVGPTIIITVIIVRYLILPVIGIGVINLAAMMGLLPPDPLFKFVLLIMFALPPGVNISTMAQLFSVGQEECAMLMMWTYLAAAFALTAWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D7P3</accession>
    <name>A0AAD5D7P3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">VOC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_014397</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010002368">
        <property type="protein sequence ID" value="KAI7754862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D7P3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07246">
        <property type="entry name" value="VOC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="entry name" value="2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054575">
        <property type="entry name" value="At5g48480-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054576">
        <property type="entry name" value="At5g48480-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC_core"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34109">
        <property type="entry name" value="BNAUNNG04460D PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34109:SF1">
        <property type="entry name" value="VOC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22650">
        <property type="entry name" value="At5g48480-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22656">
        <property type="entry name" value="At5g48480-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="1">
        <location>
            <begin position="16"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7754862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="158" mass="16614" checksum="309AE43F5B044187" modified="2024-05-29" version="1">MATEATNGASQVTFTALKPQLFVESSKVNDAVAFYKAAFGAEEVNRVSNPKRKADQELPLLLSVEIKLGSSTILVSDLSDDSTAPVKAIGTGLVFCLETEENIESVVEKAVKAGAVVDSEVTEGDGACFTGRVGKVKDPYGIVWLICSPAKKCADVEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D8C7</accession>
    <name>A0AAD5D8C7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Importin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_007220</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000501">
        <property type="protein sequence ID" value="KAI7756241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D8C7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997">
        <property type="entry name" value="IMPORTIN-7, 8, 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997:SF9">
        <property type="entry name" value="IMPORTIN-9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="4">
        <location>
            <begin position="30"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7756241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="200" mass="22119" checksum="D10E0227067DDFD2" modified="2024-05-29" version="1" fragment="single">MANIIDQDQQWLISSLNATLDTNQQARSFAEASLNQASLQPGFGSALSKIAANRDLPLGSRQISFCQYLTIFYLAAVLLKQFIKKHWQEDEDGFEPPVVSNEEKAIIRGLLLLSLDDPHRKICTAIGMAVASVAHHDWPDEWPDLLSYLMKLINDQSNTNAVNGALKCLALISADLDDKLVPRIIPVLFPCLHAIVSSPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D9K7</accession>
    <name>A0AAD5D9K7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">WAT1-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_001528</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000210">
        <property type="protein sequence ID" value="KAI7756976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D9K7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030184">
        <property type="entry name" value="WAT1-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31218">
        <property type="entry name" value="WAT1-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="42"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7756976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="127" mass="14061" checksum="F37272B96DA3743A" modified="2024-05-29" version="1" fragment="single">IAFTLYDWATRVAAKPLRVPSPFPQGERERKKEIPILMERKLVGEVVPCMLMALMEFCTIGLTILASTVLSKGLSPFVFVVYTNALASIFLLPYNTFIISRNKVTMAQNLAFVGLTYSSPIVAIGMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D9P9</accession>
    <name>A0AAD5D9P9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Interactor of constitutive active ROPs 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_031686</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ICR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010001074">
        <property type="protein sequence ID" value="KAI7755512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D9P9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029688">
        <property type="entry name" value="ICR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34224:SF17">
        <property type="entry name" value="INTERACTOR OF CONSTITUTIVE ACTIVE ROPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34224">
        <property type="entry name" value="INTERACTOR OF CONSTITUTIVE ACTIVE ROPS 2, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="121"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="182"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="25"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7755512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="314" mass="35023" checksum="B8F88A38A8EFF4F5" modified="2024-05-29" version="1" fragment="single">MQHPKTRGGSTGSGTKPKSPVPKAVDNRWQRSPTSEKKRVTKAPELETQIARLQEELKKTKSQLIEAESCTNQARLEANEAKKELAAMSAKLNEMWACEESKIQELRKISQDRDRAWEAELKAVQKHLATAINENQKLNARLEKAAEAEAEAVQAKQAGLVNEVMSLRSQLSESVNAVEEVKIRLNESKEAESRALEQLESVKSSMEEEASKAEAVWVEELGTTRAANTEMENELRRLKVQMEQWRKAAEVAAAMVLGEVGGGRKFEESSESFDFNAIGEKSNSGYLEYTEDEASIKKTSNMLKKFGVLLKIGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DAN7</accession>
    <name>A0AAD5DAN7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_016459</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000097">
        <property type="protein sequence ID" value="KAI7757568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DAN7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7757568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="221" mass="25236" checksum="DE93ED82CE216C34" modified="2024-05-29" version="1">MEVVAAKEEQEGSLFEQQPCLLSSFEQGHTELEQQWQACLASAFSVLDKLEYIGCNLTTKVEVLIISGMDVQVSYAFRCELKLQQEAVDYLDQHFNRLKHTESQKARSGQTSNMRGSSAMPQSSLSIKWKSIVCLCKEGRLVCFKVGDSVIYKRLCGGIDVINKITMVHIEQYEKQDSEDGNQHHFCLVYADVEGRRVDTWLVKDSQSHQCKQRFIDRVNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DC32</accession>
    <name>A0AAD5DC32_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">HTH myb-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_022845</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000041">
        <property type="protein sequence ID" value="KAI7757923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DC32"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051953">
        <property type="entry name" value="Plant_SW-associated_TFs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47997">
        <property type="entry name" value="MYB DOMAIN PROTEIN 55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47997:SF75">
        <property type="entry name" value="MYB DOMAIN PROTEIN 55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="7">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7757923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="170" mass="19471" checksum="97CA3015AA4DBC1B" modified="2024-05-29" version="1">MMAAKLPGRSDNEIKNRWNTHLKKRADDQDHIMLEMDHRGTNMEPIDQANLTQNPELVQQNENEGELNSTEASSTSPSSCTRTELSSCLFSNVDSQTSSQLAKDFWTEPFLEDIPFSVDSILSPLNVVDNLIAQSSCNDMMSNYDEYSWSMLDSYHEYNNEFINCWESIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DCA1</accession>
    <name>A0AAD5DCA1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">WAT1-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_028467</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the drug/metabolite transporter (DMT) superfamily. Plant drug/metabolite exporter (P-DME) (TC 2.A.7.4) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000185">
        <property type="protein sequence ID" value="KAI7757067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DCA1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030184">
        <property type="entry name" value="WAT1-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31218">
        <property type="entry name" value="WAT1-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="76"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="101"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="7">
        <location>
            <begin position="10"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7757067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="159" mass="17475" checksum="AEBD3154DA1400CB" modified="2024-05-29" version="1" fragment="single">TITVKEYPDRQTIVFFYGLFGTIQCIALSPFLERNRRAWLLQPGIRVIAVVLGAVYSSAVRNSILTWCFEKKGPVYAAMFSPLSIVLAVILGVTFLGDTLYLGSVIGAAIVAVGFYMVLWGQTKEKNKILVVKDDNMDLSGSSADQTTLLLSRVNESEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DCV2</accession>
    <name>A0AAD5DCV2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_003148</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="JAMZMK010000192">
        <property type="protein sequence ID" value="KAI7757035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DCV2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000195">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001023">
        <property type="entry name" value="Os07g0541700 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002:SF1095">
        <property type="entry name" value="G-TYPE LECTIN S-RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE RKS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002">
        <property type="entry name" value="RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE SD1-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="56"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7757035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="221" mass="25197" checksum="8E73AA4BBEF5B2CB" modified="2024-05-29" version="1">MKFGRNLKRGSVTNMTSWKSVDDPSKGEKRKCKAQKNFLDLPLFSLSKLLSATNNFSDENMIGQGGYGPVYKGYLEDGREVAVKRLSQCSTQGVEEFKNEVIFISKLQHRNLVKILGCCIEKQEKLLIYEYMPSNSLDMYIYDEGQSKKLDWSHKYQILNGIAGGLLYLHEDSRLCIIHRDLKAANILLDVDMNPKISDFGTARHFIEDETESNTKRVIGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DEI1</accession>
    <name>A0AAD5DEI1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_022842</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000041">
        <property type="protein sequence ID" value="KAI7757920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DEI1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042019530" evidence="1">
        <location>
            <begin position="23"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7757920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="98" mass="11406" checksum="78CC9D54C4F7B019" modified="2024-05-29" version="1">MQSLFLIFITFLSINFIKHCCATVFTFKMHHRFSDPVKKWSESVNNKLFANGFPDIGSVEYYSQLAEYDRVFRGRRLLPDQRLTFSDGNSSLQLTSLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G171</accession>
    <name>A0AAD5G171_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">phosphatidylinositol 3-kinase</fullName>
            <ecNumber evidence="1">2.7.1.137</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_031219</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6 7">Belongs to the PI3/PI4-kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.137" evidence="1"/>
    <dbReference type="EMBL" id="JAMZMK010012160">
        <property type="protein sequence ID" value="KAI7724794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G171"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034271">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class III, type I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034272">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class III, type II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016303">
        <property type="term" value="F:1-phosphatidylinositol-3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08397">
        <property type="entry name" value="C2_PI3K_class_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00870">
        <property type="entry name" value="PI3Ka_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00896">
        <property type="entry name" value="PI3Kc_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1010.10:FF:000002">
        <property type="entry name" value="Phosphatidylinositol 3-kinase catalytic subunit type 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1070.11:FF:000014">
        <property type="entry name" value="Phosphatidylinositol 3-kinase, root isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.70:FF:000012">
        <property type="entry name" value="phosphatidylinositol 3-kinase, root isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1010.10">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.70">
        <property type="entry name" value="Phosphatidylinositol 3-kinase, accessory domain (PIK)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011162">
        <property type="entry name" value="MHC_I/II-like_Ag-recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057756">
        <property type="entry name" value="PI3-kinase_type3/VPS34_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015433">
        <property type="entry name" value="PI3/4_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002420">
        <property type="entry name" value="PI3K-type_C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001263">
        <property type="entry name" value="PI3K_accessory_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042236">
        <property type="entry name" value="PI3K_accessory_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008290">
        <property type="entry name" value="PI3K_Vps34"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048:SF7">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3-KINASE CATALYTIC SUBUNIT TYPE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00792">
        <property type="entry name" value="PI3K_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00613">
        <property type="entry name" value="PI3Ka"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000587">
        <property type="entry name" value="PI3K_Vps34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00142">
        <property type="entry name" value="PI3K_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00145">
        <property type="entry name" value="PI3Ka"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54452">
        <property type="entry name" value="MHC antigen-recognition domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51547">
        <property type="entry name" value="C2_PI3K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00915">
        <property type="entry name" value="PI3_4_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00916">
        <property type="entry name" value="PI3_4_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51545">
        <property type="entry name" value="PIK_HELICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 6" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="C2 PI3K-type" evidence="10">
        <location>
            <begin position="14"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="PIK helical" evidence="9">
        <location>
            <begin position="275"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="8">
        <location>
            <begin position="539"/>
            <end position="805"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR000587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7724794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="820" mass="93992" checksum="3005BB5EF9EEF302" modified="2024-05-29" version="1">MTGNEFRFFLSCDINLPVTFKIERLEGRLPSTNTSTPADIDRGVEDKKPELYVECALYIDGAPFGLPMRTRLESSGPPFCWGELITLSTKYRDLTANSQLAVTVWDVSCGKNEGVVGGATIRLFNVKKQLRTGKHKLRLWQGKEADGSIHSTTPGKVPKEERGELERLEKLVNKYERGLIQRVDWLDRLAFKAMEKIKDVESSKNGSSHLYVIIDFCSFEHRVVLQESGPNLLIPASITSTNELVTVWDPEVGKINPSEHKQLKLARSLNRGIIDRDLKPSSTERKSIQRILKYPPTRTLSGDERQLLWKFRFSLMSEKRALTKFLRCVEWSDVQEAKQAIELMGRWEMIDVCDALELLSPVFESEEVRAHAVRVLERADDEELQCYLLQLVQALRFERSDKSRLSQFLVQRSLSNIEIASFLRWYVAVELRDPAYAKRFYSTYEMLEENMIKVVFCLLGMGARDGEDVFKLWQSLVRQTELTAQLCSIMRDVRNVRGGTQKKIEKLRRLLSGLLSELTYFEEPIRSPLAPIVLITGIIPSDSSIFKSALHPLRLAFRTSDGGCLKIIFKKGDDLRQDQLVVQMVSLMDRLLKLENLDLHLTPYRVLATGHDEGMMEFIPSKTLAQILSEHRSITSYLQKFHPDEEGPFGITATCLETFIKSCAGYSVITYILGIGDRHLDNLLLRDDGRLFHVDFGFILGRDPKPFPPPMKLCKEMVEAMGGAESQYYRRFKSYCCEAYNILRKSSNLILNLFHLMAGSNIPDIASDPEKGILKLQEKFRLDLDDEECVHFFQDLINESVGALFPQMVETIHRWAQYWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G1I3</accession>
    <name>A0AAD5G1I3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="5">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">M8C21_015448</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">AA19_3_7</strain>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="15">Interacts with RAP74.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="5"/>
    <dbReference type="EMBL" id="JAMZMK010012184">
        <property type="protein sequence ID" value="KAI7724587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G1I3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008420">
        <property type="term" value="F:RNA polymerase II CTD heptapeptide repeat phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009651">
        <property type="term" value="P:response to salt stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17729">
        <property type="entry name" value="BRCT_CTDP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07521">
        <property type="entry name" value="HAD_FCP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000098">
        <property type="entry name" value="RNA polymerase II C-terminal domain phosphatase-like 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10190:FF:000014">
        <property type="entry name" value="RNA polymerase II C-terminal domain phosphatase-like 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057473">
        <property type="entry name" value="ARM_CPL3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039189">
        <property type="entry name" value="Fcp1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011947">
        <property type="entry name" value="FCP1_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02250">
        <property type="entry name" value="FCP1_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23081:SF2">
        <property type="entry name" value="RNA POLYMERASE II C-TERMINAL DOMAIN PHOSPHATASE-LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23081">
        <property type="entry name" value="RNA POLYMERASE II CTD PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25505">
        <property type="entry name" value="ARM_CPL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00533">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0678">Repressor</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="FCP1 homology" evidence="18">
        <location>
            <begin position="853"/>
            <end position="1046"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="17">
        <location>
            <begin position="1089"/>
            <end position="1182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="405"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="564"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="654"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="789"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="431"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="656"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="690"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="736"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="797"/>
            <end position="815"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00063107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAI7724587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="1184" mass="130964" checksum="65374C043ECB821B" modified="2024-05-29" version="1">MGNDLLVSVVDAEEGEISDSDSVEEISEEAFAAKNPAVIRSVAESNKRPRVWTMQDLVKYQNKYQMARNYAPGLYNFAWAQAVQNKPLDHYLTNLNTDTDTTTVADNGNAKIVVDLSGDDSEKEEGELEEGEIDLVTTEVVEIGKEGEDDNDDDDVEKWIDVIRIGLEGITLNDANKSFNGVCSRLDDMLDSLSTLLSENSVSNKDDLVQLAFSAIQLVFCSMSQNQKEVSRHIISRMLSNVKSHVSVMFSPESLKEVESMFLSCNSNDACSSTKANDNEGDLPNHDQEVVSINEEPELDVMLIGSSDQNSTQSESLKIQTSTSKNRVLLPLPLLDLHKDHDVDSLPSPTRETTNVLPLGKALLVGDAEIRPEWPTPTPRVVLQHYETDALNAVSTYQQKFSRSTFLSNDLPSPTPSEESDDASGDCGGEVSSSSNVNSSFVSSATLEKRNVSSVPEMHNVQLQANGSYVAPVYSTSSTVTRVPNKTRDPRLRLANPSSSLEVSQFRVPLSSKQPIIEPLDGLQGSRKQKTFDEPVFVDGPAPKRKKIEFLESRDPRSIMAVSQTRSLENVPPTSALPSTSSGSVHIPVTSINPTSASLQSILKDIAANPTQWLHLISSIEQQRLVDPRNQVTQTSQNSLLGSTPVATVASQSLMGEKKSTDKMQKPCTSSTDDPGNLKMKPRDPRRALHSNSLQKQEPITNLSTVATNKGSSKRTDPRVLSQANKRNSKQADPRVSSQPISPPDSSYQFTKNLKNIVDVVSTTANASVQMPSSQTSPVYSSYVNIKGPSSHPGHLQNGTRSTSREVASGLGPSQSHNVWGDVEQLFGRFDDQQRAAIQRERSKRMEEQKKMFADRKLCLVLDLDHTLLNSAKIICFENPVLKICQCFQFSEIEPVHDEMLKKKEEQDRDKPHRHLFRFPHMGMWTKLRPGVWNFLEKASKLYELHLYTMGNKVYATEMAKVLDPKGVLFNGRVISRGGHGDSVDGDPKSKDLEGVLGMESAVVIIDDSIRVWPHNKPNLIVVERYIYFPCSRRQFGLSGPSLLEIDHDERPEDGSLASSLAVIEKIHQLFFLEKSLDEADARNILASEQRKILGGCRILFSRVFPVGEASPHLHPLWQTAEQFGAVCTNQIDEQVTHVVANSLGTDKVNWALATGRPVVHPGWVEASALLYRRANEHDFAVKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G218</accession>
    <name>A0AAD5G218_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein RADIALIS-like 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_016129</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012150">
        <property type="protein sequence ID" value="KAI7724828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G218"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048262">
        <property type="term" value="P:determination of dorsal/ventral asymmetry"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009908">
        <property type="term" value="P:flower development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000154">
        <property type="entry name" value="Transcription factor SRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044636">
        <property type="entry name" value="RADIALIS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43952">
        <property type="entry name" value="MYB FAMILY TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43952:SF72">
        <property type="entry name" value="MYB-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Myb-like" evidence="5">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="6">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7724828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="81" mass="9606" checksum="E7E397B99F5CBC10" modified="2024-05-29" version="1">MASWTPRQNKQFEDALAYYNKDSPDRWHKIARAVGGKSVEEVKRHYDILVRDIMRIEADQVPLPNYRDIERNGRGYGNDQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G3J3</accession>
    <name>A0AAD5G3J3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_011204</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011710">
        <property type="protein sequence ID" value="KAI7726328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G3J3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042162">
        <property type="term" value="F:telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11660">
        <property type="entry name" value="SANT_TRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057625">
        <property type="entry name" value="TPR1-6-like_ubiquitin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031105">
        <property type="entry name" value="TRP_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21717:SF86">
        <property type="entry name" value="HOMEODOMAIN-LIKE, UBIQUITIN-RELATED DOMAIN PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21717">
        <property type="entry name" value="TELOMERIC REPEAT BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23603">
        <property type="entry name" value="Ubiquitin_TPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH myb-type" evidence="6">
        <location>
            <begin position="398"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="5">
        <location>
            <begin position="401"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="17"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="95"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="109"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7726328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="509" mass="56772" checksum="6C5522093C27C3F9" modified="2024-05-29" version="1">MMSKKSICHGRCNGFEWSNVPKAPRSLRRSSHKRSSDGSKLCAFELLAAVADKLLQESESSTSSISQEPKEQAAFPIVKQEELEVKGNDVKSEIVDHGSSAESKSNLARKLDPPLKESLESDNDSGLEHVSEVTIVKNVENNVKLEGDAPRQGGLRDVNLVNHVLGITDDDENSFRCDCRGARMRAFRSRSHGGYRRVRKMLTYRYRKVTLMKDYELTNPTKLGQIEAASKRRKLFHQSGKENKTDKVKISIKSIKVPELYIEMPENASIASLKRTVMEAVRTFLGGKLHVGMSLEGKKVKDNNRTLKQIGISMNRDLDTLGFTLEPSLPYDTSRQPSSCTASPIVGVGSSNGLLNEEIVPLETEVLTEEKVAASNALVPAVPINYKQINKAEVTQCRRIRRPFSVMEVEALVEAVETLGTGRWRQVKLRAFDGADHRTYVDLKDKWKTLVHTASISPQQRRGEPMPQDLLDRVLAAHRCWSIHQVKPRKHQTAPLGILGGSSVEFVGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G492</accession>
    <name>A0AAD5G492_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">14-3-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_003423</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 14-3-3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011339">
        <property type="protein sequence ID" value="KAI7727632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G492"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.190.20">
        <property type="entry name" value="14-3-3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000308">
        <property type="entry name" value="14-3-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036815">
        <property type="entry name" value="14-3-3_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023410">
        <property type="entry name" value="14-3-3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18860">
        <property type="entry name" value="14-3-3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00244">
        <property type="entry name" value="14-3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00305">
        <property type="entry name" value="1433ZETA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00101">
        <property type="entry name" value="14_3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48445">
        <property type="entry name" value="14-3-3 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="14-3-3" evidence="3">
        <location>
            <begin position="1"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="117"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="132"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7727632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="142" mass="15747" checksum="B52063AEF9E40E00" modified="2024-05-29" version="1" fragment="single">KGDYFRYLAEYKSGEDCKDVTLQSLKAYEAATAIATSDLPPTHPIRLGLALNFSVFYYEIMASPERACSIAKQAFDDAVPELDSLDEEAYKDSSLIMQLIKDNLTLWTSDIAEEGGTPKTHTHTTHFINSCEHPKAGEPQGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G4A1</accession>
    <name>A0AAD5G4A1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycine-rich protein A3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_006577</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011243">
        <property type="protein sequence ID" value="KAI7728274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G4A1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31248">
        <property type="entry name" value="DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_5G04290)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31248:SF2">
        <property type="entry name" value="DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_5G04290)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="139"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="40"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7728274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="177" mass="18115" checksum="BDB3553F3B31F6DB" modified="2024-05-29" version="1">MSGEEHHHNNNNNTQDKGLLSNLGHAAAGYAQNHHGGGAYPPQHGYPPQQGYPPQGYPPQQGYPPQQGYPPAGYPGHQAPHSSGGHMPGMGTVLAGGAAAAAAAYGAHHIASSHGHHGGHNVSHGVQGMMPGMGHFSGGKHGKHSGGKFKAGKHGKGGKHGKFGKGKGKHSGFKKWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G567</accession>
    <name>A0AAD5G567_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_002177</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011069">
        <property type="protein sequence ID" value="KAI7729114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G567"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7729114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="84" mass="8894" checksum="81401CFF57EBAE8C" modified="2024-05-29" version="1">MEEDESEEEKDVKPVKRPAETTPKSPASAKKGKPNTPQNAKPNTPQNAKPNTPQKSGGKKGGVVTPQANSKPSFKKGKKGGNRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G5S0</accession>
    <name>A0AAD5G5S0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">9-cis-epoxycarotenoid dioxygenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_011441</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="5">Binds 1 Fe(2+) ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the carotenoid oxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011202">
        <property type="protein sequence ID" value="KAI7728488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G5S0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009570">
        <property type="term" value="C:chloroplast stroma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010436">
        <property type="term" value="F:carotenoid dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016121">
        <property type="term" value="P:carotene catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004294">
        <property type="entry name" value="Carotenoid_Oase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10543:SF123">
        <property type="entry name" value="9-CIS-EPOXYCAROTENOID DIOXYGENASE NCED5, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10543">
        <property type="entry name" value="BETA-CAROTENE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03055">
        <property type="entry name" value="RPE65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="4 5" id="KW-0408">Iron</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="5">
        <location>
            <position position="286"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="335"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="400"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="575"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR604294-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7728488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="589" mass="65530" checksum="F6608E69273077F1" modified="2024-05-29" version="1">MATNPYTSTLLQSSFSSITKPQTTQFTSIKPSKPIKFTINCAALQSSSLSSSLLHFPPSITTPQQQKQPQQPLQWNFLQRAAATALDMFENALITRETNVPLPKTSDPSVQISGNFAPVPEQSVKHNLTVSGTIPDCVKGVYVRNGANPLFEPVAGHHLFDGDGMVHAVTFDGDSVSYSCKLTKTNRLVQEKELGRPVFPKAIGELHGHSGVARLALFYARSLLGLVDHSKGMGVANAGLVYFNNRLLAMSEDDLPYHIKITPNSDIETVGRYDFSEQLASTMIAHPKIDPVTGEMFALSYDVVQKPYLKYFKFSKDGEKSPDVEIPLPVPTMMHDFAITENFVVVPDQQVVFKLQEMIKGGSPVVYDDNKMSRFGILSKTATTSDNMIWVDSPETFCFHLWNAWEEPETNEIVVIGSCMTPPDSIFNESDENLESILSEIRINLITRKTTKRRILKQSEQVNLEAGMVNRDKLGRKTRFAYLAIAEPWPKVSGFAKVDLVSGELKKYMYGDNRYGGEPFFLPNGGNSEVEDDGYVLTFVHDEKTWKSELQIIDAVTMKMEASVKLPSRVPYGFHGTFVSSKDLAQQAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G761</accession>
    <name>A0AAD5G761_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF7148 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_006141</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010431">
        <property type="protein sequence ID" value="KAI7731560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G761"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009570">
        <property type="term" value="C:chloroplast stroma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055572">
        <property type="entry name" value="DUF7148"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36352">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36352:SF1">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23650">
        <property type="entry name" value="DUF7148"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7148" evidence="1">
        <location>
            <begin position="59"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF23650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7731560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="180" mass="19594" checksum="168E46DF198CF014" modified="2024-05-29" version="1" fragment="single">LKEPVSPKPAFSYDRVSHRFRLSKSDKKWGSRLVLGGQFSGCAVVRAKPNNSITPAGDDDEGVSLGTMKLPMNTDLDRFETLLFQWANSMNQGAQIPLPMPLKVDKIKGGIRLGFITIGDGVTEVPVYIDCLVFPAADGSPPIFRAIREGPMKDRSPPGEPRIMKSLLAALQKSVQLAAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G7J0</accession>
    <name>A0AAD5G7J0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cathepsin propeptide inhibitor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_011508</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010453">
        <property type="protein sequence ID" value="KAI7731397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G7J0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2250">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013201">
        <property type="entry name" value="Prot_inhib_I29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35462">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35462:SF2">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08246">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00848">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="16"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="Cathepsin propeptide inhibitor" evidence="3">
        <location>
            <begin position="155"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="Cathepsin propeptide inhibitor" evidence="3">
        <location>
            <begin position="223"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="290"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7731397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="319" mass="36142" checksum="D355DBBD59DD930A" modified="2024-05-29" version="1" fragment="single">SPEMENGDDWLAHDKLQHFLLCFSITIIVSFLASRTKYSLLRHRSTSLASLLSLTAGAAKEFADELGFFQSAGASVKDAVADIIGVVVAVLLFQDEPALLRLADRGRAGSIVHWTQARAWGLPHNRAQYLKDIYKMEGQFEVIAAASVKSEEERFEEWMKKYNKKYESDEEKAARFKSFLANLRVVDAQCARGFNSMALNQYSDLTIDEMEGQYGAVAASDRFHDWMKKYNKIYQSHEEKAARFNSFLGNLRLVDDHYARGFNSMALDQYSDLNIDEYFREVFGCEPAPPSFTFSSSDSDDDLAEREGEARGRRLGRGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G8X4</accession>
    <name>A0AAD5G8X4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Eukaryotic translation initiation factor 3 subunit L</fullName>
            <shortName evidence="4">eIF3l</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_030694</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the eIF-3 subunit L family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009961">
        <property type="protein sequence ID" value="KAI7733510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G8X4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005852">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03011">
        <property type="entry name" value="eIF3l"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019382">
        <property type="entry name" value="eIF3l"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13242">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13242:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10255">
        <property type="entry name" value="Paf67"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3 4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="5">
        <location>
            <begin position="252"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7733510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="453" mass="53193" checksum="759E1050EC0089A3" modified="2024-05-29" version="1">MEYSEEDEGEGYDPSYVPEPIHVRDIVQDAERAFFKEKAWPSVEAVAEYVDNDHVFCLLYREMWFRHLYARLNPTLNHKIHSWDNYCTLFQLPNQWLWDMVVEFVYQFQSFSQYRAKMKTKTNHEIALLTQHDQAWNVYGVLNFLQALMEKSSIVDILQQEKEGHQQFTATDGYDYSGGSNVLKVLGYFSMVGLLRVHCLLGDYQTGLNSLLPIDITQQGVYTSVIGSHIATIYHYGFAKLMLRRYVEAIHEFNKILVYIYKTKQYHQKSPQYEQILKKNEQMYALLTICLSICPQHKLVDETVNSQLREKYGEKMLRMQRYDDESFALYDELFSYACPKFITPSAPSYDEPLVNYNQDAYRLQLKLFLYEVKQQQQLSVVRTFLKVYSTISVAKLASYMEVDEFTLRTILMTYKHKTHSVGADGKTISNPDIDFYIDDLEAVIGNMDRVKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GAJ9</accession>
    <name>A0AAD5GAJ9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_005071</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009647">
        <property type="protein sequence ID" value="KAI7734757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GAJ9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012507">
        <property type="term" value="C:ER to Golgi transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005795">
        <property type="term" value="C:Golgi stack"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048211">
        <property type="term" value="P:Golgi vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061025">
        <property type="term" value="P:membrane fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024095">
        <property type="entry name" value="Vesicle_P115"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10013">
        <property type="entry name" value="GENERAL VESICULAR TRANSPORT FACTOR P115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10013:SF0">
        <property type="entry name" value="GENERAL VESICULAR TRANSPORT FACTOR P115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7734757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="81" mass="9453" checksum="0B993D4786EC9FC1" modified="2024-05-29" version="1">MQKNKRRFGAINLMEKINSNLVCGCRKSFCIAGFWDNRLQEAILTIPHAITRLVDMLMDREVIRNEALLLLTYLTREAEEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GB94</accession>
    <name>A0AAD5GB94_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Serpin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_024394</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the serpin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009826">
        <property type="protein sequence ID" value="KAI7734111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GB94"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.497.10">
        <property type="entry name" value="Antithrombin, subunit I, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023796">
        <property type="entry name" value="Serpin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000215">
        <property type="entry name" value="Serpin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036186">
        <property type="entry name" value="Serpin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042178">
        <property type="entry name" value="Serpin_sf_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461:SF211">
        <property type="entry name" value="GH10112P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461">
        <property type="entry name" value="SERINE PROTEASE INHIBITOR, SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00079">
        <property type="entry name" value="Serpin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56574">
        <property type="entry name" value="Serpins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="domain" description="Serpin" evidence="3">
        <location>
            <begin position="61"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7734111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="185" mass="20240" checksum="97D924ED01C011DE" modified="2024-05-29" version="1">MAASWIPKRQKTLYTLLFLSNIILLAIFNGSLLGLNSKDDSYIFPSQDDLVVIRSLEQSSNQTDVAITLAAHLLSKTHDCNIVFSPLSIRVVLNLIAAGSSGQTLDQLLAFLKANTTDDLNSYSWLVSKILADGGPNGGPRLSFANGVWLDNMLSLKPSFKTVLDTVYKASSKQVNFQNKVSVKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GCU7</accession>
    <name>A0AAD5GCU7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Autophagy-related protein 11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_015534</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008989">
        <property type="protein sequence ID" value="KAI7737605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GCU7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990316">
        <property type="term" value="C:Atg1/ULK1 kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034045">
        <property type="term" value="C:phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061709">
        <property type="term" value="P:reticulophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034517">
        <property type="term" value="P:ribophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040040">
        <property type="entry name" value="ATG11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019460">
        <property type="entry name" value="Atg11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045326">
        <property type="entry name" value="ATG17-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13222">
        <property type="entry name" value="RB1-INDUCIBLE COILED-COIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13222:SF1">
        <property type="entry name" value="RB1-INDUCIBLE COILED-COIL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10377">
        <property type="entry name" value="ATG11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04108">
        <property type="entry name" value="ATG17_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0072">Autophagy</keyword>
    <keyword evidence="4 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Autophagy protein ATG17-like" evidence="7">
        <location>
            <begin position="7"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="Autophagy-related protein 11 C-terminal" evidence="8">
        <location>
            <begin position="751"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="377"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="533"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="477"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="598"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="748"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="386"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF10377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7737605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="857" mass="97175" checksum="6467CC76B89B1545" modified="2024-05-29" version="1">MKHVSNLREQRVQEKALEITRGNLDHFYKMIHQNYTDFVKCYSQQQRTHSNLLVNFGRDLERLRSIKLLPVLQTAERKCLLDFVKEDNLQKMVDDCSNSHRQFESKVGDFKQEFGELKRSTEHLFSSKASILSRDLERAVKEHEHHINEQKSILQALSKDVGLVKKLVDDCLTSQLSNSLRPHDAVSALGLMYEGHDKSYLPKMQACELAISNLLVFCKNKKTEMNIFVHKYMQKIAYIQYTIKDVRYKFSVFTEALKRQNDQFEHLKVVRGIGPAYRACLAEIVRRKASMKLYMGMAGQLAERLASRRESEIRRREEFLKVNSSFIPRDILASMGLYDASTTCDVNVAPFDTNLLDIDISDLDRFAPEHLVGLSFKKGPSSMSQDAEESSESGTENDNDFVEASELVEIAGTSKLEVENAKLKAELASTIAILCSFSPETEFEDSEVGNAPKVSAEKTAEALRLKDEHEKHLVSMLKVKHMQCESYEKRIKELENRLSDQYSLDQKHNKDPSSELMDEASYASSSLLLKSGGIDSSGTMNPNLDSSMVEPNRDEKDEKDTMVADVGMVLASSSTADYMPPPLKSLTSDDEVKASDLVVELQNLLTEKTNQLNETETKLREAMEEVTRLGSELEISRSLLEESQMNCAHLENCLHEAREEAQTHLCAADRRASEYSTLRASAIKIRSLFERLKTCVSSSGVAGFADSLPALAQSLANSANENGDDGINEFRECVRVLAEKVGVLTRQRAELIDRYTKTEASHEQLTKELEEKKDLVNTLYMKLQSEKQVNKEKISFGRLELHEIAAFVLNAAGHYEAINRNRTHYYLSSESVTLFTDHLPNRPTHIVDRLCLSRDKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GDR6</accession>
    <name>A0AAD5GDR6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Amino acid transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_013264</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008958">
        <property type="protein sequence ID" value="KAI7737769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GDR6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF698">
        <property type="entry name" value="AMINO ACID TRANSPORTER TRANSMEMBRANE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="24"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="59"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="92"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="132"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="166"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="210"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="246"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="274"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="309"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="7">
        <location>
            <begin position="4"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7737769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="338" mass="37163" checksum="499A6989BE867109" modified="2024-05-29" version="1">MDSDPLIKSYPDIGQVAFGRKGRAIVSTFLYLELFFVAVEFLIMEGDNLHKLFPKELEFVVFGTRVGGKQVFVLLTALVVLPTTWLRSLGVLAYVSAGGVLASLVLVLAVFWCGAFDGIGFHERGDVWNMSGLSTAISLFTFCYCGHAVFPTICNSMKDKSQFPKVLFVCFVLSTISYGSMAIFGYLMFGEHLTSQITLNLPTKNISSKIAIYTTLINPISKYALVISPIATAVEETVPFRESKMMSCLIRTCIVTSTVFVALVVPYFGYVMAFIGAFLSITVSILLPCLCYCKIIIGFKKFGVELMMIYMILLFGTCVAVVGTYTALTDIVKQVTPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GE34</accession>
    <name>A0AAD5GE34_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF547 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_009042</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009134">
        <property type="protein sequence ID" value="KAI7737051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GE34"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006869">
        <property type="entry name" value="DUF547"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46248">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46248:SF6">
        <property type="entry name" value="OS03G0859900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04784">
        <property type="entry name" value="DUF547"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF547" evidence="1">
        <location>
            <begin position="2"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7737051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="120" mass="13497" checksum="BEF0525AE1A3B7B5" modified="2024-05-29" version="1">MNPNGIFALCCGTRSSPAVRVYTSDGVVNELERAKLEYLQASIIVTSAKKIGLPELLLRHMHDFAQDLESLVEWLCQQLPTSGSLRKSMVDCFRGINNANVSSIVEKLPYEFEFQYLLPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GEI6</accession>
    <name>A0AAD5GEI6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">G-box binding protein multifunctional mosaic region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_010733</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008628">
        <property type="protein sequence ID" value="KAI7739390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GEI6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044827">
        <property type="entry name" value="GBF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012900">
        <property type="entry name" value="MFMR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45967">
        <property type="entry name" value="G-BOX-BINDING FACTOR 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45967:SF40">
        <property type="entry name" value="HOMOSERINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07777">
        <property type="entry name" value="MFMR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G-box binding protein multifunctional mosaic region" evidence="2">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="139"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7739390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="153" mass="16573" checksum="10B97A1FD0A68218" modified="2024-05-29" version="1">MGTGEKRSPAKQSKPTSSQETPPSSYLDWSSSMQTLNFGSEAVPSFFASSTVTSPVPHPYMWGGQHPMMPPYGAPVPYPYVYPPPGVYGHPNMPLIPGAVQPNTEMETKASTGKDEVKKKSKETCGNVNDIGVGANKKRKEDQVDQDEDNKIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-10-08" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GHP7</accession>
    <name>A0AAD5GHP7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_021715</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007823">
        <property type="protein sequence ID" value="KAI7743065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046394">
        <property type="term" value="P:carboxylic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.10.10:FF:000002">
        <property type="entry name" value="D-alanine aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.10:FF:000008">
        <property type="entry name" value="D-amino-acid transaminase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.10.10">
        <property type="entry name" value="D-amino Acid Aminotransferase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001544">
        <property type="entry name" value="Aminotrans_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036038">
        <property type="entry name" value="Aminotransferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043132">
        <property type="entry name" value="BCAT-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043131">
        <property type="entry name" value="BCAT-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050571">
        <property type="entry name" value="Class-IV_PLP-Dep_Aminotrnsfr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42743">
        <property type="entry name" value="AMINO-ACID AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42743:SF22">
        <property type="entry name" value="D-AMINO-ACID TRANSAMINASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01063">
        <property type="entry name" value="Aminotran_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56752">
        <property type="entry name" value="D-aminoacid aminotransferase-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7743065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="370" mass="40613" checksum="E081125B9243998A" modified="2024-05-29" version="1">MAFAFMKKPISGSGRGASLQFRFPNPPNLPLQFPQFRFRLFPASFLLNTSSLIQTSSHAHDLPILSAPQAIERLRTSRESGERTQRYLAMYSSVFGGIILDEGAMVIPIDDHMVHRGHGVFDTAAIVDGYLYELEQHLDRFLGSASKAKINPPFDKETTKRILVQTASASSCKNGSLRYWLSSGPGDFQLSPSGCHKSSLYAVVIQDQSPPSFKGINVITSSVPIKPPQFATVKSVNYLPNVLSKMEAEENGAYAAIWLDEGGFVAEGPNMNVGFVTRENELLMPRFEKILSGCTAKRVLELVEDLVQKGKLRGVQVRDVSVEEGKEAQEMMLIGSGVVVRPVLQWDXXXXAMVSSAFAFETQLVFGSGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GIW8</accession>
    <name>A0AAD5GIW8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glycolipid transfer protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_016591</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007534">
        <property type="protein sequence ID" value="KAI7744460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GIW8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902387">
        <property type="term" value="F:ceramide 1-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902388">
        <property type="term" value="F:ceramide 1-phosphate transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3520.10:FF:000004">
        <property type="entry name" value="Glycolipid transfer protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3520.10">
        <property type="entry name" value="Glycolipid transfer protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036497">
        <property type="entry name" value="GLTP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014830">
        <property type="entry name" value="Glycolipid_transfer_prot_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10219:SF84">
        <property type="entry name" value="GLYCOLIPID TRANSFER PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10219">
        <property type="entry name" value="GLYCOLIPID TRANSFER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08718">
        <property type="entry name" value="GLTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110004">
        <property type="entry name" value="Glycolipid transfer protein, GLTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycolipid transfer protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7744460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="201" mass="22895" checksum="696484F24CE6CF90" modified="2024-05-29" version="1" fragment="single">MEETVFTSAIEGMKHMKSEHDEILTKPFLDVCKMLLPILDKFGAAMVVVKSDINGNIARLDSKYTESPTRFNYLYALVQPELETKTAKNSSSCTNGLLWLTRAMDFLVELFRNLYQHRDWTMQQACIDSYGKTLKKWHGWIASSSFTVAIKLVPDRKKFMEVIASKGDTYADMVIFCESFSPVLAQIHKFLHSCGVDSMKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GJI1</accession>
    <name>A0AAD5GJI1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">1-aminocyclopropane-1-carboxylate synthase</fullName>
            <ecNumber evidence="8">4.4.1.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_033940</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>S-adenosyl-L-methionine = 1-aminocyclopropane-1-carboxylate + S-methyl-5'-thioadenosine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21744"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:58360"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="4.4.1.14"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="7">Alkene biosynthesis; ethylene biosynthesis via S-adenosyl-L-methionine; ethylene from S-adenosyl-L-methionine: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.4.1.14" evidence="8"/>
    <dbReference type="EMBL" id="JAMZMK010007337">
        <property type="protein sequence ID" value="KAI7745165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GJI1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016847">
        <property type="term" value="F:1-aminocyclopropane-1-carboxylate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009693">
        <property type="term" value="P:ethylene biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050478">
        <property type="entry name" value="Ethylene_sulfur-biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43795:SF6">
        <property type="entry name" value="1-AMINOCYCLOPROPANE-1-CARBOXYLATE SYNTHASE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43795">
        <property type="entry name" value="BIFUNCTIONAL ASPARTATE AMINOTRANSFERASE AND GLUTAMATE/ASPARTATE-PREPHENATE AMINOTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00753">
        <property type="entry name" value="ACCSYNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0266">Ethylene biosynthesis</keyword>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="10">
        <location>
            <begin position="2"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7745165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="332" mass="37981" checksum="1755A8F9DA4ECBCA" modified="2024-05-29" version="1" fragment="single">FDRDLKWRTGVELIPVVCESSNNFKVTIEALEDAYEKAKESNIKVKGLLITNPSNPLGTFFDKETLKTLVRFINEKNIHLICDEIYAGTVTNGSEFVSIAEILEEVPTICDHSLIHIVYSLSKDMGFPGFRVGIIYSYNDTVVNISRKMSSFGLVSTQTQHMIASMLSQNDFVDNFIQESRTRLAHRHDMFTRELAEVGIGSLESNAGLFFWMDLRRFLKEPTFESEMTFWRIIINEIKLNVSPGSSFHCNEPGWFRVCFANMDDATVMVAIERIKSFVLETRMLEIKTKALAKAKKQCWQNNLTLKLSSRRLEDIMSPHSPLNSPMLRAQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GMY1</accession>
    <name>A0AAD5GMY1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glycoside hydrolase family 5 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_032793</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 5 (cellulase A) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006451">
        <property type="protein sequence ID" value="KAI7748872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GMY1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001547">
        <property type="entry name" value="Glyco_hydro_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31263">
        <property type="entry name" value="CELLULASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_5G14560)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31263:SF44">
        <property type="entry name" value="OS04G0481200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00150">
        <property type="entry name" value="Cellulase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="Ricin B-like lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycoside hydrolase family 5" evidence="5">
        <location>
            <begin position="98"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7748872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="580" mass="64725" checksum="21EBA533E386F0B4" modified="2024-05-29" version="1">MYIICILYFSTSKYHTTKPINMGWNISFSSTLPLLLLLLATTLNHHHLTAALPLSTNSRWIVDDGEGGERVKLSCVNWVSHLDAVVAEGLSKQPVDVISKKILAMGFNCVRLTYPLFLFTNESLASVTVRQSLKKLGLIESVAGFQANNPSFIDLPLIKAFQEVVASLDRNNVMMVLDNQISKPGWCCSDFDGNGFFGDRYFDPDVWLKGLTKVATMFNSTKNVVGMSLRNELRGPKQNISLWYRYMQKGAEVVHAANPNVLVIFSGLSYDKDLSFIQTQDVSLTFSNKLVFEAHWYGFSNSEEWETGNPNQVCGRIVDNITGKSGFLLEKGYPLFISEWGVDQRGTNEDDNRYLNCFIAWAADNDLDWAIWTLAGSYYFREGVVGMEEFYGVLNWDWCEPRNSSFLNKISSIQSPFQGPGLSNTRPHKIIFHPATGLCVRRQSVFKPLTLGPCAEPEHWDYTSKQTLTIKGTYYCLQTDGVGKTAKRGIICTDSSSKWEAISASKLHLSSKTNNGTVVCLDIDSENNIVTNNCRCLTNDNGCDPASQWFKIINSTIGAATESYSRLDAILQSLGANLLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GNA7</accession>
    <name>A0AAD5GNA7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Amino acid transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_033844</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the amino acid/polyamine transporter 2 family. Amino acid/auxin permease (AAAP) (TC 2.A.18.5) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006412">
        <property type="protein sequence ID" value="KAI7749065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GNA7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF692">
        <property type="entry name" value="TRANSMEMBRANE AMINO ACID TRANSPORTER FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042092008" description="Amino acid transporter transmembrane domain-containing protein" evidence="9">
        <location>
            <begin position="22"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="69"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="113"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="149"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="176"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="210"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="10">
        <location>
            <begin position="8"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7749065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="237" mass="25525" checksum="8442AD88D9AD2746" modified="2024-05-29" version="1" fragment="single">GGVVTLAAVVVCLLWLGVVDGIEYHPSRTALNFGNLPVAVGLIGFCYGSHSVFPNIYTSMKEPSRYPSALLISFSVSFVMYTAVGVYGYLMFGDSVKSQFTLNMPTHYVSSKVAAWTVVVAPVTKFALTLTPIAYGIEELLPPAQQNSYVVSMMIRTTLMILILLVALTLPYFGVVMALTGSVLEMLVSIIFPCACYLRLLHGHTTTTEITLCSLMILLGLVCAIVGTYTSLTSIPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GPW2</accession>
    <name>A0AAD5GPW2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_006312</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006450">
        <property type="protein sequence ID" value="KAI7748899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GPW2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7748899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="54" mass="5667" checksum="59F7A2FEB3AEA84F" modified="2024-05-29" version="1" fragment="single">MAIANVVLMKNNCTSSFDDIQANFGSEVGSDSKGVTYSAHRSIGIALFSLATVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GQG8</accession>
    <name>A0AAD5GQG8_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_000357</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006571">
        <property type="protein sequence ID" value="KAI7748243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GQG8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000118">
        <property type="term" value="C:histone deacetylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.960.30:FF:000001">
        <property type="entry name" value="F-box-like/WD repeat-containing protein TBL1XR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000218">
        <property type="entry name" value="WD40 repeat-containing protein HOS15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.960.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045183">
        <property type="entry name" value="Ebi-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22846:SF2">
        <property type="entry name" value="F-BOX-LIKE_WD REPEAT-CONTAINING PROTEIN EBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22846">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08513">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00667">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2 7" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="262"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="321"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="362"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="445"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="487"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="538"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="100"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="197"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="113"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7748243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="626" mass="71604" checksum="FCEA82A60DE8AC54" modified="2024-05-29" version="1">MINMNSHDLNYLVYRYLHESGFTHTAFTLGYEAALNKSTIDGNVVPPGALVTFVQKGIQYLELEANLTCNDSDIDEDFSFIQPIDLITKNVYELQQMIKEKKQNLPKTKHRERNRDNKEHEQEHSREKDREHSREKEKEHAKGREKEIEQEHAREREKERQHVREREKKMEIEIYREREQEKEKHIRDKERELEKEKIEREKDKAKQKEKKNEDVADVNRDEDKIRVQHEENVTPEGADPMEVDNNSAPMSALIPNSDVTVLKGHTSEVFVCAWSPTESLLASGSGDSTARIWTIRDGPCRSDSQYGPSNVSVLKHYRGRTNDKSKDVTTLDWNGEGTLLATGSYDGQARIWSKDGELVSTLTKHKGPIFSLKWNKKGDYLLSGSVDKTAIVWDIKTGEWKQQFEFHAAPTLDVDWRNNVSFATCSTDNMIYVCKVGDTRPVKTFAGHQGEVNAIKWDPTGTLLASCSDDSTAKIWSLKQDTCLHDFKEHTKEIYTIRWSPTGPGTNNPNQPLVLASASFDSSIKLWDVETGRLLHSLVAHGDPVYSVAFSPNGEYLASGSLDKCMHIWSVKEARVVKTYEGSGGIFEVCWNKQGDKIGACFSNNVVCILDFRMRLDAFRFRTKRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GRI6</accession>
    <name>A0AAD5GRI6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_029513</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006098">
        <property type="protein sequence ID" value="KAI7750669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GRI6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35277">
        <property type="entry name" value="OS09G0363700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35277:SF10">
        <property type="entry name" value="OS09G0363700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7750669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="111" mass="12151" checksum="E448971828DFD956" modified="2024-05-29" version="1" fragment="single">LIGPVINDSPSKEVQLSGSSVKAHKERKLQIAMADIRSEPTKSQEEEAKAPNLIEKGKEEVKKSASHDKETHGTSNDIDEDTPVEKVKGPGILERTKEEIEAVVEAINPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GRM3</accession>
    <name>A0AAD5GRM3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_017181</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006244">
        <property type="protein sequence ID" value="KAI7749966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GRM3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31482">
        <property type="entry name" value="ESTS AU081301(E20138)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31482:SF2">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="4"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7749966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="329" mass="38203" checksum="9AF927BD685B8585" modified="2024-05-29" version="1">MNEGMSVLDLPNLALECILERLEPAGLCKMACVCSYLRDMCSSDHLWENHMKKRWGRIIGYAAVKEWQLQVATNKESNYFLAGDKGRGILMSYLCKLWPVMLIRPNSTSYIDDHDHDHDVTEMKVSSQPSVDSIVSCYRALETGKFWFPAQVFNRENGHVGFVLSCYDADLSYDSHTDTFQARYPPHGRRAAAIEIGVSWDRLRAPPVETSPHDLHISDCLNDLRPRDHIEIQWRRNKQFPYGWWYGVVGHLEACDGNISYCHCHESDTVVLEFNQYTPGSRWRHTMINRAYHREEGNEADGFYGGIRKLYNKDEISMWQQFWPAEILE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GRZ3</accession>
    <name>A0AAD5GRZ3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Sister chromatid cohesion 1 protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_019778</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the rad21 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006265">
        <property type="protein sequence ID" value="KAI7749893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GRZ3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008278">
        <property type="term" value="C:cohesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051754">
        <property type="term" value="P:meiotic sister chromatid cohesion, centromeric"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21793">
        <property type="entry name" value="Rad21_Rec8_M_AtSYN1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.580">
        <property type="entry name" value="Structural maintenance of chromosome 1. Chain E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039781">
        <property type="entry name" value="Rad21/Rec8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006909">
        <property type="entry name" value="Rad21/Rec8_C_eu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006910">
        <property type="entry name" value="Rad21_Rec8_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023093">
        <property type="entry name" value="ScpA-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12585">
        <property type="entry name" value="SCC1 / RAD21 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12585:SF64">
        <property type="entry name" value="SISTER CHROMATID COHESION 1 PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04824">
        <property type="entry name" value="Rad21_Rec8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04825">
        <property type="entry name" value="Rad21_Rec8_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rad21/Rec8-like protein N-terminal" evidence="6">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="Rad21/Rec8-like protein C-terminal eukaryotic" evidence="5">
        <location>
            <begin position="532"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="146"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="318"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="417"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="154"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="165"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="189"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7749893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="583" mass="64620" checksum="820ABCAD3B8EA3C9" modified="2024-05-29" version="1">MALRLSGILMGGVVIVYERKVKLLYEDVTRLLVEINEAWKVKAKRDPTVLPKGKSQAKYEAVTLPDNKPADLGDFEQLLQFSNSDTLMGMHKSAYIDMNLDNIDESYISHNQGDANLPHDHHQADIENITLFDRFDSHQTDAGRFNQFERFDIEGDDMDRDDETLEKNPSHNHSHDLPNTLVPSPPLQDEPKIPDEIHEQHPGYDINHQSDEDKAQHIPARQKPSRKRARRVPSVIMDYEQTMIPGHMYQSWLQDASDLVSRRGRKIKHSGSIRSIKTERLMDLPPVALVDGLFTNRNGDHYYPLPLIKQWTKLTQPVHDLPSGETSPPHGPHPPAPSSSSPPEANNYHDPTTIPEDHHSGVGSGSLGISIEKQMPDVNNHEIPEIISTLMNNGINASGANGGPVAGADMAVTPANSGGGVKSMSSESGPGFVPEASDTTSGRSNKKRLFSSSRKSGSGLEPVAEEFAFNHTDLNFKLAQLSENELSPEKLIVESGPTQTQKVPPPEQPMEQLTDSIRKQLKAHFDTPGCAQTESLNQLAMGLDRKKAACLFYQTCVLATRDIIRVQQKEPYGNIIISRGAKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GT99</accession>
    <name>A0AAD5GT99_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Purple acid phosphatase</fullName>
            <ecNumber evidence="9">3.1.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_004093</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a phosphate monoester + H2O = an alcohol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15017"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:67140"/>
            <dbReference type="EC" id="3.1.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4 9">Belongs to the metallophosphoesterase superfamily. Purple acid phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.2" evidence="9"/>
    <dbReference type="EMBL" id="JAMZMK010005859">
        <property type="protein sequence ID" value="KAI7751661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GT99"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003993">
        <property type="term" value="F:acid phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00839">
        <property type="entry name" value="MPP_PAPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.380">
        <property type="entry name" value="Purple acid phosphatase-like, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041792">
        <property type="entry name" value="MPP_PAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039331">
        <property type="entry name" value="PAPs-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025733">
        <property type="entry name" value="PAPs_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015914">
        <property type="entry name" value="PAPs_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008963">
        <property type="entry name" value="Purple_acid_Pase-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22953">
        <property type="entry name" value="ACID PHOSPHATASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22953:SF7">
        <property type="entry name" value="PURPLE ACID PHOSPHATASE 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14008">
        <property type="entry name" value="Metallophos_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16656">
        <property type="entry name" value="Pur_ac_phosph_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49363">
        <property type="entry name" value="Purple acid phosphatase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Purple acid phosphatase N-terminal" evidence="12">
        <location>
            <begin position="50"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="10">
        <location>
            <begin position="146"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="Purple acid phosphatase C-terminal" evidence="11">
        <location>
            <begin position="351"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF14008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF16656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7751661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="444" mass="50706" checksum="E196B948A3729232" modified="2024-05-29" version="1">MQKLQLLFDYHIFLISILFLVRPHYASVDFVRQPPRPIVFTPHNRPESHPQQVHVSLVGNNYMKVSWVTDEHRVPSMVDYGTTPGKYEYSATGEHTSYRYFFYSSGKIHHVTIGPLSPGTTYYYRCGGSGPEFNFRTPPATFPLEFAVAGDLGQTGWTTTTLKHINSKDYDVLLLPGDLSYADTQQPLWDSFGRLVEPHASRRPWMVTQGNHELETFPIIDPNGFKAYNTRWPMPYEQSGSKSNLYYSFDVVGTHIIMLGSYADFTPGSDQYKWLANDVAKIDRARTPWVVVIIHAPWYNSNLAHQGEGESMRVAMEEMLYNSRVDIVFAGHVHAYERFTRIYDNNADPCGPIYITIGDGGNREGLAMLYKEPKPSFSIYREASFGHGRLRILNNTHAHWSWHRNNDTTAVVADEAWFQSLTSSSSCISKIQPRVQLTKINDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GTB4</accession>
    <name>A0AAD5GTB4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_020084</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010003756">
        <property type="protein sequence ID" value="KAI7754405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GTB4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37731">
        <property type="entry name" value="PEPTIDE TRANSPORTER FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37731:SF1">
        <property type="entry name" value="PEPTIDE TRANSPORTER FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="117"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="39"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="67"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="118"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7754405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="282" mass="31564" checksum="19ADCC2EA7E69F2A" modified="2024-05-29" version="1">MLTQTLDDKDLDDDALWAAIDSAASSALSSVTRPLKPSNNNNHNNTTTNNNRFHSPKVTFPVISKFTSPSPSPTITNPRKHSLSNSEESPNSKKIARFSLAEATSPPAPLAIVKHVQRTPTTPSSYRSDYQMQSPVNSPSDCSVMTHSLSGRFPTVDLFKEYQNAAMAILEKGDYTMISGNQFIKKSGWRKISFYFNISYEIKDKTIEFDDNRNVQRAEFIVRAHMQGGRFSDGWGSCDRREKRFLKPNHDIPSTAETRAKNKACQDLLGIGEYRPGVNAQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GUP3</accession>
    <name>A0AAD5GUP3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cytochrome P450 78A5-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_011985</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005090">
        <property type="protein sequence ID" value="KAI7753909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GUP3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11076">
        <property type="entry name" value="CYP78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000016">
        <property type="entry name" value="Cytochrome P450 78A5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051996">
        <property type="entry name" value="Cytochrome_P450_78A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47946">
        <property type="entry name" value="CYTOCHROME P450 78A7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47946:SF21">
        <property type="entry name" value="CYTOCHROME P450-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8 9" id="KW-0349">Heme</keyword>
    <keyword evidence="6 8 9" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="8">
        <location>
            <position position="465"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7753909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="518" mass="57656" checksum="C5CF98853DCDD602" modified="2024-05-29" version="1">MSSSIPSLCIPNIADSSSVISFSLFLSIVVFVSVFAFVLAPGGLSWALSKPRPKTAIPGPSGVPVLGLVFAFTSSLTHRTLANLSNAFNAKPLMAFSVGLTRFVISSHPDTAKEILNSSDFADRPVKESAYELLFHRAMGFAPYGDYWRNLRRISSTHLFNPKRVSGFGVFRENIGLKMMEQVSFSMKQNGVVEVKKILHYASLNNVMKTVFGQCYDEFGQGGDGYKLEKLVSEGYVLLGIFNWSDHFPVVRWFDVQGVRRRCRDLVSKVNVFVGEIIQQHRERRSCGGDVGVHDGDFVDVLLDLESDNKFSDADMIAVLWEMIFRGTDTVAILLEWILARMVLHPDIQARAQSEIARVVGNRPVTDSDLDNLPYLQAIVKETLRVHPPGPLLSWARLSTQDTHVGPHFVPAGTTAMVNMWAITHDERVWSEPNRFNPDRFLKEDVAIMGSDLRLAPFGAGRRVCPGKVMGLATVHLWLAQLLQKFNWVEAGSVDLSECLKLSLEMKKPLVCKAVSRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GVJ8</accession>
    <name>A0AAD5GVJ8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_001522</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000210">
        <property type="protein sequence ID" value="KAI7756970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GVJ8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000097">
        <property type="entry name" value="Chaperone protein dnaJ 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052812">
        <property type="entry name" value="Plant_DnaJ_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44272">
        <property type="entry name" value="DNAJ DOMAIN (PROKARYOTIC HEAT SHOCK PROTEIN)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44272:SF10">
        <property type="entry name" value="DNAJ DOMAIN, CHAPERONE J-DOMAIN SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="6">
        <location>
            <begin position="15"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="348"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="348"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7756970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="388" mass="43879" checksum="9D3C86FA2D6C1687" modified="2024-05-29" version="1">MPGQRSKSEKNNRRDPYEVLGVSVTSTDQQIKTAYRKLALKYHPDKNANDPKAADMFNEITYSYNILSDPDKRYQYDSAGFEAVEAETQDLELDLSSLGAMSTMVAAFFSKLGVPIKTLVSATVLEEAINGTVPTLPLALGKPVSRKVEKQCAHFYSVRITEKEAQAGIVCQVQSAEKSKFKLLYFERDENGGLNLALQEDSAKKGKVTTAGMYFLGFPVYKLDQQSSIMAKKDPDTAFFRKLEGFQPCEITELKPGVHTFAVYGDNFFKSASYTIEMLSTASFAKEKENLRAVEAHISTKRTEISKFETEYRQVLAQFTDMTSRYKQEMQTIDELLKQRNEIQATYTTAPQINSSSSSNKKRVILQDDKKAKKKWYNLHVKVDKRKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GW31</accession>
    <name>A0AAD5GW31_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein FAR1-RELATED SEQUENCE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_025608</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Putative transcription activator involved in regulating light control of development.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the FHY3/FAR1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010003837">
        <property type="protein sequence ID" value="KAI7754376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GW31"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004330">
        <property type="entry name" value="FAR1_DNA_bnd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031052">
        <property type="entry name" value="FHY3/FAR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018289">
        <property type="entry name" value="MULE_transposase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006564">
        <property type="entry name" value="Znf_PMZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31669">
        <property type="entry name" value="PROTEIN FAR1-RELATED SEQUENCE 10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31669:SF218">
        <property type="entry name" value="PROTEIN FAR1-RELATED SEQUENCE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03101">
        <property type="entry name" value="FAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10551">
        <property type="entry name" value="MULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04434">
        <property type="entry name" value="SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00575">
        <property type="entry name" value="ZnF_PMZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50966">
        <property type="entry name" value="ZF_SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SWIM-type" evidence="7">
        <location>
            <begin position="532"/>
            <end position="568"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7754376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="727" mass="82457" checksum="09BBA1F0B0653985" modified="2024-05-29" version="1">MDVGLMSLEQDSMGQHGVDFDRFDEFTNGADPAGISPSQNKISETDPHVGLEFAYVDAAKSFYNEYAKRSGFTILVNQNTGSTPDVTIATFDFLCSASGNSCNAMLRIEAKDQNSWVVTKFIKEHNHPLTNTRKMLNHHRQKRHFPSATKNNTSESYPIAGVVPNGVMYLSRSASMESNHGAISDSVTNSIRHAISKRSLGRDAQNLLDYFRKMQAENPGFYYAIQLDDDNRMANVFWADARSRTAYSHFGDTVTFDTVYRVKRYKVPFAPFTGVNHHGQTILFGCALLLDESECTFTWLFKTFLAAMNDRAPVSIITDPEKSIQSAVSHVFPGTRHCFNKWDVLREGHKRLAYVCHMHPNFQVELYNCINSTDTIDEFETMWDCILDRYDLKRNDWLQLLYSTRQQWVPAYFRDTFFAGIILNQSFESSFFDGYLNEHTTVPLFFRQYERALENSFEKEIEADFDTISTTPTLRTPSPMEKQAASLYTKTVFLKFQEELVETFVYTANRIDGDGTVSTFRVAKFEDDQKAYVVTLNAGDLRANCSCQMFEYSGILCRHILTVFTVTNILTLPSHYILKRWTKNAKSNISLDLYVNGPESLNIRYNQLIREAIKFAEEGAVAPDTFTVAMAALREGVKNVSAAKLNVANFAPPPSSRVNVIGYDNRKLGSSAPDMTPLLWPRQDEVSRRFNLNDGGLLAAPATDLNLPRMAPVSLRRDDGHPDNMVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GWH7</accession>
    <name>A0AAD5GWH7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF4408 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_007414</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000033">
        <property type="protein sequence ID" value="KAI7758050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GWH7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025520">
        <property type="entry name" value="DUF4408"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008480">
        <property type="entry name" value="DUF761_pln"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33098">
        <property type="entry name" value="COTTON FIBER (DUF761)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33098:SF84">
        <property type="entry name" value="DUF4408 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14364">
        <property type="entry name" value="DUF4408"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05553">
        <property type="entry name" value="DUF761"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="16"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="53"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4408" evidence="3">
        <location>
            <begin position="48"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="224"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="224"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="242"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7758050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="315" mass="36675" checksum="F7F3056CFA5EFABF" modified="2024-05-29" version="1">MMASSSTNSWLNSLKLLFITTGVISLAITAKLTVPLMLNFAVNDLPTMMKPPYLCLIINCIILTIVATSRFQTNHRHENQSPPLDSTPDAVQMSFHVMEPPRVGYDIIKPCVTVEHNNNKCYEKDVETVVVEDQEDELITTKDIWHPTINDQPEIKFQVEEKQLVTSSHRRKPAKHMQEGERALRVLKPKKHETLDNTWKMITESRNKPSARQFRKSDTFVNLHHDRPSHESTKTVNTKAVRKSETVKERKAYENQNHHPQSQAAEEKLKRYLSPSHEELNRRVEEFIKKFNEDMRLQRQESLNQYMEMINRGGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GWW2</accession>
    <name>A0AAD5GWW2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_021488</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 2 3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010001391">
        <property type="protein sequence ID" value="KAI7755246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GWW2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044559">
        <property type="entry name" value="WOX13-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46777">
        <property type="entry name" value="WUSCHEL-RELATED HOMEOBOX 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46777:SF5">
        <property type="entry name" value="WUSCHEL-RELATED HOMEOBOX 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 3" id="KW-0371">Homeobox</keyword>
    <keyword evidence="2 3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="5">
        <location>
            <begin position="80"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="2">
        <location>
            <begin position="82"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="142"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="147"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="167"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7755246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="255" mass="28571" checksum="B765CF2886CA6D87" modified="2024-05-29" version="1">MEQDEGNNNNTNNNNNNGGMFVKVMTDEQMEVLRKQIAIYATICEQLVDLHKSLTSHHDLAGVRLGNIYNDPLVTAGGHKFSGRQRWTPTPVQLQILERLFEQGNGTPSKQKIKEITSELSQHGQISETNVYNWFQNRRARSKRKQQVSAPNNNGESEVETEVESPKPQQNTASTADDNMCFQNPNACSVDQRAKKVEPVFPSDASSKSAGNVGQMSYYGSMLSNPRMDRIIGKMEVPGSYQQSYMQGDEYNMTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GX83</accession>
    <name>A0AAD5GX83_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">TOM1-like protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_033814</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TOM1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010001014">
        <property type="protein sequence ID" value="KAI7755576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GX83"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14231">
        <property type="entry name" value="GAT_GGA-like_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03561">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004152">
        <property type="entry name" value="GAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038425">
        <property type="entry name" value="GAT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044836">
        <property type="entry name" value="TOL_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002014">
        <property type="entry name" value="VHS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45898:SF4">
        <property type="entry name" value="TARGET OF MYB PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45898">
        <property type="entry name" value="TOM1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03127">
        <property type="entry name" value="GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00790">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89009">
        <property type="entry name" value="GAT-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50909">
        <property type="entry name" value="GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50179">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="VHS" evidence="7">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="GAT" evidence="8">
        <location>
            <begin position="121"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="89"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="353"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="353"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7755576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="569" mass="61879" checksum="BDF1112530513C81" modified="2024-05-29" version="1" fragment="single">FTVFKLLETMMKNCGDFVHLHVAERDVLHEMVKIVKKKPDFHVKEKILILIDTWQEAFGGARARDPQYYAAYHELLRLGAVFPQKADRPAPVLTNPQTQSSSSSSYANNPENGNEAAESSEFPSLSLTELQNARGIMDVLAEMLTAIDPEKKEGLRQEVIIDLVEQCRTYKQRVVHLVNSTSDESLLCQGLALNDDLQRVLAKHEALISGTSQKAKPETSSQALVPVEASLIDTGDTKQSPAAVGSSSSTGTGVEADLLAITTVTTNAAAEVNPKFDLLSGDDFSSENSLAIVPMPMGEPESTTTASQQHDALALVDILSQNNNVPSAPVLAGQTAHQFQPSQSLDSLYTNAYTSNTPYDPQTPFNLGSTSSWNGHLSQQQPPSPPAAFGSEGLPPPPWEAQSMDDNKLGSDQGMSMGMGMGMYNQQPMSATHIPPLNNQFVYPQQQFQGQQIMGGMGMIPQQQIQPGQAHMYPQQLAQQMYANQMPQPPPPPYGYGYGYGYAQQPNTQQFIDQRMSGLSLNNNATTVYKGPSMANASYVHVPSGKPTKAEDKLFGDLVDFSKVKPNKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5H0B3</accession>
    <name>A0AAD5H0B3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_018699</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000036">
        <property type="protein sequence ID" value="KAI7757978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5H0B3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7757978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="71" mass="8183" checksum="9184BD6089E77B3D" modified="2024-05-29" version="1" fragment="single">IQHPHATQQSKTAHKKLIRFSDRNDRNQLQPYEIPEKLLKTRNNTKTMQTTVGRKGATQQAFWSHLAATGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-07-31" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4P8PS26</accession>
    <name>A0A4P8PS26_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative flowering locus T</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="4">Putative terminal flower 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">TFL1</name>
        <name evidence="3" type="synonym">FT</name>
        <name evidence="2" type="ORF">M8C21_011452</name>
    </gene>
    <organism evidence="4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2018-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Virag E."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the phosphatidylethanolamine-binding protein family.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005992">
        <property type="protein sequence ID" value="KAI7751077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="MK098073">
        <property type="protein sequence ID" value="QCQ83094.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="MK098078">
        <property type="protein sequence ID" value="QCQ83099.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4P8PS26"/>
    <dbReference type="SMR" id="A0A4P8PS26"/>
    <dbReference type="OrthoDB" id="2506647at2759"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00866">
        <property type="entry name" value="PEBP_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.280.10:FF:000001">
        <property type="entry name" value="Terminal flower 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.280.10">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008914">
        <property type="entry name" value="PEBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036610">
        <property type="entry name" value="PEBP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035810">
        <property type="entry name" value="PEBP_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11362">
        <property type="entry name" value="PHOSPHATIDYLETHANOLAMINE-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11362:SF9">
        <property type="entry name" value="PROTEIN FLOWERING LOCUS T-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01161">
        <property type="entry name" value="PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49777">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7751077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="QCQ83094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="QCQ83099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="175" mass="19733" checksum="9830717C14D950CC" modified="2019-07-31" version="1">MSRRERDPLVVGRVIGDVIDNFTRTINLTVSYNDREVNNGCTLKPSQVVNQPRVDIGGDDLRAFHTLVMVDPDAPSPSDPNLREYLHWLVTDIPATTGARFGQEVVCYESPRPTMGIHRMVFVLFRQLGRQTVYAPGWRQNFNTRDFAELYNLGSPVAAAYFNCQRESGSGGRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BKA2</accession>
    <name>A0AAD5BKA2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_021015</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012152">
        <property type="protein sequence ID" value="KAI7724817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BKA2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035266">
        <property type="term" value="P:meristem growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010073">
        <property type="term" value="P:meristem maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044622">
        <property type="entry name" value="PCN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45086">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN PCN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45086:SF1">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN PCN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7724817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="135" mass="14910" checksum="081A4FCFACCDA49E" modified="2024-05-29" version="1">MFELHKCSSSSIEWKPSPIVALATSLDDSHVATAREDASLEIWLVSAASVGWHCQLRIEGDGNMRVSSLVWCGSGRLFSSCIDGSVLEWDLFNLTQKNAFGCCIFILIKVMEVSSLDACCYDGLPMDLSFKPLWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BLR8</accession>
    <name>A0AAD5BLR8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">TATA element modulatory factor 1 TATA binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_016611</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011872">
        <property type="protein sequence ID" value="KAI7725732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BLR8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022092">
        <property type="entry name" value="TMF_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022091">
        <property type="entry name" value="TMF_TATA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47347">
        <property type="entry name" value="GOLGIN CANDIDATE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47347:SF2">
        <property type="entry name" value="GOLGIN CANDIDATE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12329">
        <property type="entry name" value="TMF_DNA_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12325">
        <property type="entry name" value="TMF_TATA_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TATA element modulatory factor 1 TATA binding" evidence="5">
        <location>
            <begin position="486"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="50"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="109"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="206"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="295"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="351"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="510"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="562"/>
            <end position="589"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF12325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7725732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="602" mass="68786" checksum="FD9B73E99B0DC020" modified="2024-05-29" version="1">MVVVVVAEGVGAVAMAAEVMDWFSGKVSLGNFPDLSEARKSNEAEVESLREEYHQRVSTLERKVYALTRERDTLRREQNKKSDAAALLKEKDEIITQVMAEGEELSKKQAAQEATIRKLRAQIRELEEEKKGLITKLQVEESKVESIKRDKAATEKLLQETIEKNHAELATQKEYYTNALSAAKEAEALAESRANDEARVELEIRIKESEEREAMLVQTLEELRQTLSRKEQQAVFREDMLRRDIEDLQKRYQESERRCEELVTQVPESTRPLLRQIEAIQETSARKAEAWSAVERSLNSRLQEAEAKAAGAEERERSVNERLSQTLSRINVLEAQISCLRTEQTQLTKSLEKERQRAAESRQDYLALKEEADTNEGHVGQLQQEISQLKQKHKQELHEALTHRELLQQDIEREKNARLELEKAVLEQNKIARTKSSTENGLTRRLSSASSLSSMEESFYLQASLDSSDTLSERRNTGETSYYLKSKTPNAFEAALRQKEGELASYMSRLASMEAIRDSLAEELVKMTAECEKLRSEVSQLPGMKAEMEALRRRHSAALELMGERDEELEELRADIVDLKEMYREQVNILVNKVLSSSIGAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BLY6</accession>
    <name>A0AAD5BLY6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">alpha-mannosidase</fullName>
            <ecNumber evidence="4">3.2.1.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_022276</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides.</text>
            <dbReference type="EC" id="3.2.1.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyl hydrolase 38 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.24" evidence="4"/>
    <dbReference type="EMBL" id="JAMZMK010011843">
        <property type="protein sequence ID" value="KAI7725853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BLY6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004559">
        <property type="term" value="F:alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006013">
        <property type="term" value="P:mannose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10810">
        <property type="entry name" value="GH38N_AMII_LAM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.50:FF:000003">
        <property type="entry name" value="Alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1180:FF:000015">
        <property type="entry name" value="Alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1360:FF:000001">
        <property type="entry name" value="Alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.110.10:FF:000001">
        <property type="entry name" value="Alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.110.10">
        <property type="entry name" value="Glycoside hydrolase 38, N terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.50">
        <property type="entry name" value="Glycoside hydrolase family 38, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.30">
        <property type="entry name" value="Golgi alpha-mannosidase II, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041147">
        <property type="entry name" value="GH38_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015341">
        <property type="entry name" value="Glyco_hydro_38_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037094">
        <property type="entry name" value="Glyco_hydro_38_cen_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000602">
        <property type="entry name" value="Glyco_hydro_38_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027291">
        <property type="entry name" value="Glyco_hydro_38_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028995">
        <property type="entry name" value="Glyco_hydro_57/38_cen_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050843">
        <property type="entry name" value="Glycosyl_Hydrlase_38"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048534">
        <property type="entry name" value="Man2a1-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11607">
        <property type="entry name" value="ALPHA-MANNOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11607:SF61">
        <property type="entry name" value="ALPHA-MANNOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17677">
        <property type="entry name" value="Glyco_hydro38C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01074">
        <property type="entry name" value="Glyco_hydro_38N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21260">
        <property type="entry name" value="Laman-like_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00872">
        <property type="entry name" value="Alpha-mann_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88688">
        <property type="entry name" value="Families 57/38 glycoside transferase middle domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Glycoside hydrolase family 38 central" evidence="12">
        <location>
            <begin position="279"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7725853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="734" mass="82644" checksum="D07C55945DC04076" modified="2024-05-29" version="1" fragment="single">CVNYVCCGYDEYETGGGIVEGKLNVHLVPHSHDDVGWLKTVDQYFVGSNNSIQGACVENVLDSVVMSLLRDPNRKFIFAEMAFFRRWWLLQSKKIKDQVKKLVSAGQNGGWCMHDEAATHYIDMIDQTTLGHSLIKSNFNVTPRAGWQIDPFGHSAVQAYLLGAELGFDSIHFARIDYQDRSKRKDDKSLEVIWRGSETFGSSSQIFTNVFPRHYSAPDGFNFEVSGDFEPVQDSPFLFDMNVKKRVNDFIDAALTQANVTRTNHIMWTMGDDFQYQYAESWFKQMDKLIHYVNKASRQLEYLAGRKNKGPNTFSLGDALGIAQHHDAVTGTAKQHTTDDYEKRLAIGASEAEAVVNSALSCLTSSTSNCTLSTTKSTFTQVIVAYNPLGWKRTDIIRLPVKDANFVVQDNQGNTIEAQFVEFDEVTRNLRSFYTEAYLGFSPHDVPKYWLLFQAVVPPLGWNTYFINQKSGKGGHISMLDTPQVDTVVIGQGSLKMLIYQYNKVISGMVAQLITRLQEHTFSIPMDHLQLLFQDQRMLYDDGRGVGEPLDESVCNKTTCRGLTIRGNYYMSVNKVGSGSRWRRTTGQEIYSPLLLAFAHENQQHYKASHSTISTTMDPNYSLPPNVALITLQELEDGSVLLRLAHLYEAAEDVAYSTLAKVELKKLFAHKTIKKIKETSLSANQDKSAIKRMPWKVEGRTETGPPTVRGGPVDPLALVVELGPMEIRTFILQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BMC2</accession>
    <name>A0AAD5BMC2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Importin subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_006948</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the importin alpha family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012149">
        <property type="protein sequence ID" value="KAI7724834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BMC2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061608">
        <property type="term" value="F:nuclear import signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000222">
        <property type="entry name" value="Importin subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024931">
        <property type="entry name" value="Importin_alpha"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23316">
        <property type="entry name" value="IMPORTIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00514">
        <property type="entry name" value="Arm"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005673">
        <property type="entry name" value="Importin_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR005673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7724834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="455" mass="49386" checksum="11B9FC011E7B4FCF" modified="2024-05-29" version="1">MMIEEEQSILEAQTASAVEELKAAIAFQGKGAVQKKVNALRELRRLLSRSEFPPIETAIASGAIPLLAQCLSFGSQDEQLLEAAWCLTNIAAGKPEETRSLLPALPLLIAHIGEKSSMLVAEQCAWALGNVAGEGEDLRHILISQGALLPLAKMMFPNKGSTVRTAGWALSNLIKGPNPKAATELIKIDGVVEAILRHLKKSDEEIATEIAWVVVYLSALSPVATSVLVKTDLLQLLVERLVTSDSLQLLIPVLRSLGNLIAGDTYTTNAVLVPAHEITDNIIRALVKCLKSEHRVLKKEAAWVLSNIAAGSIGHKQLIYKSEAMPLLIQLLATSTFDIKKEVAYVVGNLCVAPAEGSGRSSLIFDHLGSFVRSGCLRGFIDLVRSADIEAARLGLQFMELVLRGLPNGEGQKLVEKEDGIDAMERFQFHENEDLRNMANQLVDKYFGADYGLDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BP34</accession>
    <name>A0AAD5BP34_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Asparagine synthetase [glutamine-hydrolyzing]</fullName>
            <ecNumber evidence="9">6.3.5.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_024773</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>L-aspartate + L-glutamine + ATP + H2O = L-asparagine + L-glutamate + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12228"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58048"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.5.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (L-Gln route): step 1/1.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.4" evidence="9"/>
    <dbReference type="EMBL" id="JAMZMK010011844">
        <property type="protein sequence ID" value="KAI7725843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BP34"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004066">
        <property type="term" value="F:asparagine synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006529">
        <property type="term" value="P:asparagine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01991">
        <property type="entry name" value="Asn_synthase_B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00712">
        <property type="entry name" value="AsnB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000055">
        <property type="entry name" value="Asparagine synthetase [glutamine-hydrolyzing]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000024">
        <property type="entry name" value="Asparagine synthetase [glutamine-hydrolyzing]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006426">
        <property type="entry name" value="Asn_synth_AEB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001962">
        <property type="entry name" value="Asn_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050795">
        <property type="entry name" value="Asn_Synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033738">
        <property type="entry name" value="AsnB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01536">
        <property type="entry name" value="asn_synth_AEB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006949">
        <property type="entry name" value="PRK09431.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11772">
        <property type="entry name" value="ASPARAGINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11772:SF48">
        <property type="entry name" value="ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING] 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00733">
        <property type="entry name" value="Asn_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13537">
        <property type="entry name" value="GATase_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001589">
        <property type="entry name" value="Asn_synthetase_glu-h"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 10" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 10" id="KW-0061">Asparagine biosynthesis</keyword>
    <keyword evidence="5 9 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 10" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 9 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="13">
        <location>
            <begin position="2"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="active site" description="For GATase activity" evidence="10">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="269"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="343"/>
            <end position="344"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for beta-aspartyl-AMP intermediate formation" evidence="12">
        <location>
            <position position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR001589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001589-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR001589-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR001589-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7725843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="591" mass="66408" checksum="5EC03CF27FED4561" modified="2024-05-29" version="1">MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQHGDNYLAHQRLAIIDPASGDQPLFNEDETVVVTVNGEIYNHEELRKSLTLTGHKFRTGSDCDVIAHLYEEHGEDFVDMLDGMFSFVLLDTRNNTYIAARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFEVFPPGHLYSSKTGGFKRWYNPRWFAETIPSTPYDPLVLRRAFENAVIKRLMTDVPFGVLLSGGLDSSLVASITARYLAGTKAAKQWGAQLHSFCVGLEGSPDLKAAREVADYLGTVHHEFHFTVQDGIDAIEDVIYHIETYDVTTIRASTPMFLMSRKIKSLGVKMVISGEGSDEIFGGYLYFHKAPNKEEFHEETCRKIKALHQYDCLRANKSTSAWGLEARVPFLDKEFINVAMSIDPEAKMINMDEKRIEKWILRRAFDDEEHPYLPKHILYRQKEQFSDGVGYSWIDGLKAHAELHVTDRMMLHAGNIFPHNTPVTKEAYYYRMIFERFFPQNSAKLTVPGGASIACSTAKAIEWDASWSNNLDPSGRAALGVHNAAYKKNTGPVGSVKLAPNIIDNVPRMMDISASELVIQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BP71</accession>
    <name>A0AAD5BP71_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_013965</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HscB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011856">
        <property type="protein sequence ID" value="KAI7725794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BP71"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044571">
        <property type="term" value="P:[2Fe-2S] cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051259">
        <property type="term" value="P:protein complex oligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.20:FF:000002">
        <property type="entry name" value="HscB mitochondrial iron-sulfur cluster co-chaperone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000082">
        <property type="entry name" value="Iron-sulfur cluster co-chaperone protein HscB, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.20">
        <property type="entry name" value="HscB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004640">
        <property type="entry name" value="HscB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036386">
        <property type="entry name" value="HscB_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009073">
        <property type="entry name" value="HscB_oligo_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00714">
        <property type="entry name" value="hscB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14021">
        <property type="entry name" value="IRON-SULFUR CLUSTER CO-CHAPERONE PROTEIN HSCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14021:SF15">
        <property type="entry name" value="IRON-SULFUR CLUSTER CO-CHAPERONE PROTEIN HSCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07743">
        <property type="entry name" value="HSCB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47144">
        <property type="entry name" value="HSC20 (HSCB), C-terminal oligomerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="7">
        <location>
            <begin position="119"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7725794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="278" mass="32303" checksum="5C3A63EDF81F6AC1" modified="2024-05-29" version="1">MNLSMNKMWKKTLLKTPLSTVNRRTLTQSIPLQQNIIQSHNQPPFSTLCTLNTTKQTIISTNPNTHFIWSSLHLGNSSRFCFSQSFNEPSLCCWNCKTQKPFLICESCGCVQPVDQKVDYFKIFGLERKFDIEDGSLEGMYKDWQKKLHPDLVHTKSKEEREYAADQSARVIDAYTTLRKPLLRAIYLMRLEGVEVDEEQTVSEPELLGEIMEIREAVEEAADSQALNKIQSQMEEKLRHWSKSFAYAFGGKNYDEAIKSIQRMTYYHRVNEEIVKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BPG1</accession>
    <name>A0AAD5BPG1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_033500</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011411">
        <property type="protein sequence ID" value="KAI7727201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BPG1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08771">
        <property type="entry name" value="DLP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1240">
        <property type="entry name" value="Dynamin, middle domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022812">
        <property type="entry name" value="Dynamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001401">
        <property type="entry name" value="Dynamin_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045063">
        <property type="entry name" value="Dynamin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000375">
        <property type="entry name" value="Dynamin_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030381">
        <property type="entry name" value="G_DYNAMIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003130">
        <property type="entry name" value="GED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020850">
        <property type="entry name" value="GED_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566">
        <property type="entry name" value="DYNAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566:SF186">
        <property type="entry name" value="DYNAMIN CENTRAL DOMAIN, GTPASE EFFECTOR DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01031">
        <property type="entry name" value="Dynamin_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00350">
        <property type="entry name" value="Dynamin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02212">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00053">
        <property type="entry name" value="DYNc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00302">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51718">
        <property type="entry name" value="G_DYNAMIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51388">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0505">Motor protein</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynamin-type G" evidence="6">
        <location>
            <begin position="1"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="GED" evidence="5">
        <location>
            <begin position="548"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7727201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="644" mass="72693" checksum="94915E9C8C402F79" modified="2024-05-29" version="1">MANTDPNQSNNGEVIHPFTDDDHNQKRSSLAVSYNDKIRPILDAVDKLRRLNVTQEGIPLPTIVVIGDQSSGKSSVLESLAEYQKKSVKIVEESKICEEISRATVEIAGNSKGISNVPLTLVVKKKGVPDLTMVDLPGITRVPVGDQPKDIHEQISGMIMEYIKPEESIILNVLSASDDFTTCESICMSRLVDNSGQRTLAVVTKCDQSPNGLFEKVTKNEVNIGLGYICVRNRINDETYEEARNQEAALFETHPLLAKIDKSMVGIPVLAHRLVQIQSVIISKCLPDIVKKINERLNASVLDLNKLPRVLKSEADAMATFIQISGSLKETLQKIIIQGEIDEYEDDKQMHCDARLAEMLDKLSKDLKASVKFCESFLVEEIQFLEEVNGIRLPHFIPHSVFLRLLKKKVNSVSDLPINFVNKVWDFLEIVCAKVLMDRCGNYPQLLPSMKKATQYVMSKMKAKFVERVTEMIEMEKITDYTCDPNFITSYNKLMSCRDTFQDSFVSINSVSGPSFGRSPSYVFHVEFYGKIVTKHLTDVPEKTREQAFDLKMRMTAYWNIVLQRMVDSLALQLRFFIQKVVNKEMEMEIVNEVLVHGGGIEKMLNEPPSVAKKRERLENSISLLQESKETMEQVMDGLVVSSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BPH5</accession>
    <name>A0AAD5BPH5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DUF3444 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_004524</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011438">
        <property type="protein sequence ID" value="KAI7727115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BPH5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024593">
        <property type="entry name" value="DUF3444"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056988">
        <property type="entry name" value="Zn_ribbon_pln"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45089">
        <property type="entry name" value="DNAJ HEAT SHOCK AMINO-TERMINAL DOMAIN PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45089:SF57">
        <property type="entry name" value="DNAJ HEAT SHOCK N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11926">
        <property type="entry name" value="DUF3444"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23551">
        <property type="entry name" value="Zn_ribbon_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zinc beta-ribbon" evidence="3">
        <location>
            <begin position="52"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="DUF3444" evidence="2">
        <location>
            <begin position="258"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="domain" description="DUF3444" evidence="2">
        <location>
            <begin position="571"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="93"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="503"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="133"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="209"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="514"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="526"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="548"/>
            <end position="558"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7727115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="776" mass="87735" checksum="11951E1313B7476C" modified="2024-05-29" version="1">MILSDNGKRSIYDYMSRDPAKFTFGKPQGTHTSHSGAQNNNSNAHHQPQPLFWTFCPFCKANYEFYRDQVNKRIKCPECSKLFIAIDIGKSTKAPHAGKQNVNPRTNPSFSHPAYQCAKTGDAKVDTQRAEPSFSSSECQPPQCTKTGDAEVGGTVENGDIKTKGGENKGTGGNPSDASDSNGKRTECSKQSRKRARVSNSEETVADFSTEKRYRLRKKSSDVKDKQNEPKEVPADAELKEENNKSDSDSEDELEPVYVDVPDLEFSNFDEDKEEHCFAVDQIWAVYDTIDCMPRFYAQIRKVYSSGFRLRITWLEADPEDPLEKKWAEAGLPVTCGKFKHGDSEETKDRLMFSHQMVFEKGSKRSTFVIYPKKGEIWALFKDWDIAKWSSDPGNHMKYKFEIVEILSDFDKDNGVLVAYMVKVEGFVSLFQKTSRVKLAEYRVPSSDLYRFSHRVPSLKLTGNERADVPVGSWELDTASLPDDLDQFYFSINVKVKLESVSSGQMGASFPQPPEKKVRSKHDLDQSTGVQNTSNIIAMPAKGNNPSRQDDDVTECTKTHPVSSSSDDKEPKISIHDFNLDKQNWIFKTGQIWALGRSKNVNLRGYAQIKKIESSPLRLHVDLLESCDDTAGPYGCGIYKASTGERHIIQQDLFLYLVKAQLNGRNYFNIYPSKNEIWVLCKEWDAEYTFADVDAGNYDIVEVIENNGEIIKVLLLTHVGGYKSVFKGVDRVVDIPIDESHRFFYQVSAVVLTDEQDGQLRGFWELDLAEFPGTKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BPI3</accession>
    <name>A0AAD5BPI3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_004221</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011408">
        <property type="protein sequence ID" value="KAI7727233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BPI3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22160">
        <property type="entry name" value="F-box_AtFBL13-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053781">
        <property type="entry name" value="F-box_AtFBL13-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006566">
        <property type="entry name" value="FBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050232">
        <property type="entry name" value="FBL13/AtMIF1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055411">
        <property type="entry name" value="LRR_FXL15/At3g58940/PEG3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31900">
        <property type="entry name" value="F-BOX/RNI SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31900:SF30">
        <property type="entry name" value="SUPERFAMILY PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08387">
        <property type="entry name" value="FBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24758">
        <property type="entry name" value="LRR_At5g56370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="19"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7727233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="461" mass="52947" checksum="535DF9E62D45D177" modified="2024-05-29" version="1">MSESRHHKQLEIEHTDDMIDYISNLPDCVLHRILSFLPTKEVVKASVSSKRWKNMWTSVPSLDFDDTLLYTSEVDCQHSRESTSFINFVERILRLRDASTIEKFRLSCRVWFNSPRICSWISSAIMHNVRELDLCIFAEDSSIIPQSLFDCKSLVILKIEMDCVIEIPPRVSFPCLKTLHLSLVTFPNDDSTEKLFSSCVVLEELVLLDCDCANLKNFTLSSLTLQRLTIDDLPVYEPNGCKIKIEAKNLTYLEYIGYLSNDIFLSNVLSLVKASIHIPILHGRQKEVGCRAVDLLKGLQNVVFLKVSNRTMECLIFAGGMLDRFPVFPKLTHLVLTMELGNYTFKAFIDFLCSCPNLQSINLFEGFDRCMRLGASDLIWLLVPICISQSLKALTFKNFHADDSEICFLKCVLKYARVLEKMDVWWCKTESRDLKKRKDVRKELELEIIEKGSAACIINFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BQ00</accession>
    <name>A0AAD5BQ00_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">CASP-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_006198</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3 8">Homodimer and heterodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Casparian strip membrane proteins (CASP) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011367">
        <property type="protein sequence ID" value="KAI7727471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BQ00"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006702">
        <property type="entry name" value="CASP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33573">
        <property type="entry name" value="CASP-LIKE PROTEIN 4A4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33573:SF57">
        <property type="entry name" value="CASP-LIKE PROTEIN 4B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04535">
        <property type="entry name" value="CASP_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="91"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="Casparian strip membrane protein" evidence="10">
        <location>
            <begin position="52"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="12"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7727471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="140" mass="15635" checksum="FE5BBCF79A895C5B" modified="2024-05-29" version="1">MSSFPSSPPQEKMSQPTPPSASVESQPQTPLPPPPGYGVTGVLRRWRHEDVLKRGSLGLRVLGFLFSLLAFIIMASNKHGRGRNFDEYEEYRYALAIAILSTVYTGLQSWRQIHEMSTGKFLFSGLNLALIDFFGDQVCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BR62</accession>
    <name>A0AAD5BR62_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cathepsin propeptide inhibitor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_028297</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011284">
        <property type="protein sequence ID" value="KAI7727950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BR62"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2250">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013201">
        <property type="entry name" value="Prot_inhib_I29"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08246">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00848">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cathepsin propeptide inhibitor" evidence="2">
        <location>
            <begin position="15"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="123"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="137"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7727950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="174" mass="20316" checksum="CE8955D40BEFBD7B" modified="2024-05-29" version="1">MSESKAQEDRDRVAFEHWKLKWNKTYATSEEENYRFQNYLRTLRRVGGDSANDDDDDKSSPSWWALSRYADLTTDEFKFEMFGFNSVTDHFSSDSDSDDEMLAKTHIKAYQLVRLEDGTSRIEISYPSTTSPPHKHQRDDEKDTNKKEEKEPPLKLQKIGCDGKNNKLPGCFFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BS17</accession>
    <name>A0AAD5BS17_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_002210</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010011186">
        <property type="protein sequence ID" value="KAI7728613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BS17"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056760">
        <property type="entry name" value="RING_XB3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24128:SF14">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE XBAT31-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24128">
        <property type="entry name" value="HOMEOBOX PROTEIN WARIAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24921">
        <property type="entry name" value="RING_XB3-XBAT31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10 11" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="repeat" description="ANK" evidence="11">
        <location>
            <begin position="46"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="11">
        <location>
            <begin position="80"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="11">
        <location>
            <begin position="114"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="11">
        <location>
            <begin position="159"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="11">
        <location>
            <begin position="196"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="13">
        <location>
            <begin position="324"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7728613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="447" mass="48227" checksum="3FF526AB39B21902" modified="2024-05-29" version="1">MGQGLSCKVVQDEHGLFTAVQYGHLDIVNNLLLKDPSLIHQTTVYDRHSALHIAAANAQIEIVCLLLKNGSVNPDCLNRHKQTPLMLAAMHGKIACVEKLIEAKANILMFDSLNGRTCLHYAAYYGHSDCLEAILSAARTSHVAASWGFSRFVNIRDGKGATPLHLASRQRRSQCVHMLLDNGALVSASTGGYGFSGSTPLHLAARGGSIDCIRELLAWGADRLHRDASGRIPYLVALKHKNEACAALLNPSSAAPLVWPSPLKFISELNQDAKALLEQALMEANRAREKTILKGAGYSLQSPSHSDTGTVDDNTSEASETELCYICFDQVCTIEVQDCGHQMCAQCTLALCCHNKPDPTTASVTAPVCPFCRSNIARLVVAKVKVNDFDNDLDLYPSCKLPKLRKARNFSEGSSSFKGISGVSSFGKMVGRGSGKVSVDSECIDKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BSS1</accession>
    <name>A0AAD5BSS1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_020061</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011172">
        <property type="protein sequence ID" value="KAI7728654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BSS1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7728654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="26" mass="2905" checksum="338EFE46C1E4435B" modified="2024-05-29" version="1">MGMFDQFHKVPETGYGSLQTCQEPCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BSY7</accession>
    <name>A0AAD5BSY7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein mago nashi-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_005866</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mago nashi family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011303">
        <property type="protein sequence ID" value="KAI7727881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BSY7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035145">
        <property type="term" value="C:exon-exon junction complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11295">
        <property type="entry name" value="Mago_nashi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1560.10:FF:000001">
        <property type="entry name" value="Protein mago nashi homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1560.10">
        <property type="entry name" value="Mago nashi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004023">
        <property type="entry name" value="Mago_nashi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036605">
        <property type="entry name" value="Mago_nashi_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12638:SF0">
        <property type="entry name" value="MAGO HOMOLOG, EXON JUNCTION COMPLEX SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12638">
        <property type="entry name" value="PROTEIN MAGO NASHI HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02792">
        <property type="entry name" value="Mago_nashi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89817">
        <property type="entry name" value="Mago nashi protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7727881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="156" mass="18100" checksum="E42B06374CEFA7D8" modified="2024-05-29" version="1">MAEEENGGGAAAGEFYLRYYVGHKGKFGHEFLEFEFRPDGKLRYANNSNYKNDTMIRKEVFLTPSVLKECRRIVSDSEIMKEDDNNWPEPDRVGRQELEIVMGNEHISFTTSKIGSLMDVQTSKDPEGLRIFYYLVQDLKCFVFSLISLHFKIKPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BUM7</accession>
    <name>A0AAD5BUM7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_029879</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010862">
        <property type="protein sequence ID" value="KAI7729993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BUM7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7729993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="52" mass="5899" checksum="DD7D95CE62043275" modified="2024-05-29" version="1" fragment="single">SLKVDAFPITVMFGMCSAFLFLASMLQRRLLGVAERSRQDKVRELEAEPLNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BW39</accession>
    <name>A0AAD5BW39_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_016660</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010978">
        <property type="protein sequence ID" value="KAI7729548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BW39"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009506">
        <property type="term" value="C:plasmodesma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004714">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045272">
        <property type="entry name" value="ANXUR1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27003">
        <property type="entry name" value="OS07G0166700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27003:SF475">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="16"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7729548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="230" mass="26305" checksum="337DB7C6DE613D56" modified="2024-05-29" version="1" fragment="single">LKHLRIPFEDIKEATQNFTTLIGRGGYGPVYRGQLSLSGEHMVVAVKRLQSDHRSGQGLKEFLTEIQLLSRYKHPNIEEAPELLELEGALEVRKLVETLQIQQFEEACEVQELEEALGLHKLVETLQLQQFTEACEMNELEEALGLRKLVETLQQHEFEETLKLQEEARKFQASYDYKLVGDLESEIGASRIDYEVLKAIYDMPDTPLSYIASDQIYSHLSRGILVDNGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BWF9</accession>
    <name>A0AAD5BWF9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">HSF-type DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_009843</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Homotrimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the HSF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010942">
        <property type="protein sequence ID" value="KAI7729681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BWF9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000037">
        <property type="entry name" value="Heat stress transcription factor B-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000232">
        <property type="entry name" value="HSF_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015">
        <property type="entry name" value="HEAT SHOCK TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015:SF329">
        <property type="entry name" value="HEAT STRESS TRANSCRIPTION FACTOR B-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00447">
        <property type="entry name" value="HSF_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00056">
        <property type="entry name" value="HSFDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00415">
        <property type="entry name" value="HSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0346">Stress response</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HSF-type DNA-binding" evidence="12">
        <location>
            <begin position="6"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="100"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="150"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="102"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="117"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7729681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="275" mass="30637" checksum="6BD1AA059676899F" modified="2024-05-29" version="1">MIHRSNPAPFLTKTYLMVDDPETDDVISWNDIGTSFIVWKTADFARDLLPNSFKHNNFSSFVRQLNTYGFRKTVPDKWEFANENFKRGHKELLIEIRRRKTATASHANTQDKPAGEDGPTSSSDDHGSSSTSSPDSKNPGSIDTPTVEELETLSDENNKLKQEKQVLKTELEQMKKQCDDLVAFLTKNVKVAPDQIDRIMAGEAVTGEMAMQDGGVRNGDQEVEGNGFKLFGVVLKEENKKRGRDEINVCSGAQRKNMKGVNMCAATGQSTKVCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BWV4</accession>
    <name>A0AAD5BWV4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DEUBAD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_032303</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010681">
        <property type="protein sequence ID" value="KAI7730694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BWV4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21865">
        <property type="entry name" value="DEUBAD_NFRKB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044867">
        <property type="entry name" value="DEUBAD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024867">
        <property type="entry name" value="NFRKB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13052">
        <property type="entry name" value="NFRKB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13052:SF2">
        <property type="entry name" value="NUCLEAR FACTOR KAPPA-B-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51916">
        <property type="entry name" value="DEUBAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEUBAD" evidence="4">
        <location>
            <begin position="87"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="207"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="250"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="471"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="220"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="265"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="507"/>
            <end position="518"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7730694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="831" mass="93334" checksum="8665C6CE0CECAF35" modified="2024-05-29" version="1">MAADKLNKRSKVSNGICHTSWEIQKVKKKYPHYDLNTKSRICLEWDDKTKSVVSKKEQISIAQRELTPFLPFVAHHQNAIGDVFAAPPELFELNNLTGLLSYEVWQTHLSVQEREFLTQFLPEGDEPHAVVHELLAGNNLCFGNPFQKWGASICSGDYHPDAILRQEQRNKASKVAYYSELQEYHTKMIGSLQSWKETWTRSMNPENIMQKTPRPKRDFRKSGSSHEIDVQNGPQHDFCVTSGSCSWNADDKSCNSDSPEAPDLSASNGETLVSASRTDLGNRSVARPRKGDKLRKLNVESVDGAKYMSYIKVSKEQHERVKSSMKQSNTSVQPRSLNLVLGNLDGFCVQPYELFEEEERQKLHDYWLHLAKKDLPAGFENWKSWRSAKWQLTMSLRKEMEDKWKSNDRPVLNLHNNQDDEFITDGQAEELEHSSEEQHDGEETNDAPLIALEENMEQNDISVFRSEEDINAENHDATNKSSQNYTENHDATNKSSPDYAENHDATNKSSQDSGQHQNFCPVPINTHSDAITESDAFPSSLVDYPESINHADASVGEQFPLPSAAAEIWPLAPLPNVYYHQPTSVSQDYPSVSQPSFLNRRSDSGDSFFNPYASQAQDRNELLLHSLYKDPGNSHYLGTSQFSRNLSLPLFSDPRLNIQQNIYTDGPGRFPIQREEPLLPLDQVQGWPGTGVVNLPMSAPSHHRSSQNWFSDDEVVRDGWSGGVVPNHDISSGGQVVDENLYSVLSECNGLRSGVHYGSTEIMQPGNYVEVGRESVLPSTANGVPPRAARGPGLNYMSGNEAQLGWMNLPGSLQDSGGKPFARLWNGRSLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BYJ0</accession>
    <name>A0AAD5BYJ0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Thaumatin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_001591</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the thaumatin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010400">
        <property type="protein sequence ID" value="KAI7731700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BYJ0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09218">
        <property type="entry name" value="TLP-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.110.10:FF:000002">
        <property type="entry name" value="Thaumatin-like protein 1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.110.10">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037176">
        <property type="entry name" value="Osmotin/thaumatin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001938">
        <property type="entry name" value="Thaumatin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017949">
        <property type="entry name" value="Thaumatin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31048">
        <property type="entry name" value="OS03G0233200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00314">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002703">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00347">
        <property type="entry name" value="THAUMATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00205">
        <property type="entry name" value="THN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49870">
        <property type="entry name" value="Osmotin, thaumatin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00316">
        <property type="entry name" value="THAUMATIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51367">
        <property type="entry name" value="THAUMATIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042206626" description="Thaumatin-like protein" evidence="4">
        <location>
            <begin position="23"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="31"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="79"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="94"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="146"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="151"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="159"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="180"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="190"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR002703-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7731700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="243" mass="26045" checksum="EA09078B59C4649D" modified="2024-05-29" version="1">MKPMHLPIFLLLITTVTTIVSATTIMMVNKCNHPVWPGIQPGAGHPVLARGGFKLPPKKSYTLFLPPNWSGRLWGREGCTFDHSGHGKCATGDCGGTLFCNGAGGAPPATLAEITLTAEQDFYDVSLVDGYNLPIAITPFKGTGKCSYAGCVSDLNVMCPVGLQVRSRDKRRVVACKSACSAFNSPRFCCTGSYGNPQTCKPTAYSKIFKVACPRAYSYAYDDPTSIATCTGANYLLTFCPHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BYS5</accession>
    <name>A0AAD5BYS5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Shikimate O-hydroxycinnamoyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_032219</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the plant acyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010364">
        <property type="protein sequence ID" value="KAI7731855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BYS5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050266">
        <property type="term" value="F:rosmarinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.10:FF:000015">
        <property type="entry name" value="Spermidine hydroxycinnamoyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.10:FF:000008">
        <property type="entry name" value="Tryptamine hydroxycinnamoyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050317">
        <property type="entry name" value="Plant_Fungal_Acyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31642:SF11">
        <property type="entry name" value="SHIKIMATE O-HYDROXYCINNAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31642">
        <property type="entry name" value="TRICHOTHECENE 3-O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02458">
        <property type="entry name" value="Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7731855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="416" mass="46635" checksum="B9508BFC18770CC9" modified="2024-05-29" version="1">MVRPAEETPTRKLWISSLDLMAPNYHTPVVYFYRPNGAANFFDTKVMKDALSRVLVAFYPMAGRFIHDQHGRVEIDCQGQGVLFLEAESDGMVDDFGDFAPQMEFLKLVPAVDYSLGIESFPLLVLQVTYFKCGGVSLTVAMHHYLADGPSALHFINTWADMAHGLDITLPPFIDRTLLRALDPPQPVFEHVEYHPYPTMPLQVPSDETNTVYSIFKLTRDQLNLLKAKSKEDGNTISYSSFEILSGHVWKCVCKARGLLDNQGTKLDIPTDGRARLQPPLPPGYFGNAIFTTAVITTAGEIQSKPTWYAASIIHNALVTMNSDYLKSALGYLEQRLDHKPKESYKYTNLRIISWARLLIHEADFGWGRPIFMGPTSIPLGCCYFLPSPINDGSLSIIIGLEAEQMKLFSNLLYAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BYY1</accession>
    <name>A0AAD5BYY1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_017006</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010361">
        <property type="protein sequence ID" value="KAI7731895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BYY1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14686">
        <property type="entry name" value="bZIP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031106">
        <property type="entry name" value="C/EBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23334:SF49">
        <property type="entry name" value="BASIC LEUCINE ZIPPER 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23334">
        <property type="entry name" value="CCAAT/ENHANCER BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07716">
        <property type="entry name" value="bZIP_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BZIP" evidence="2">
        <location>
            <begin position="89"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="370"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7731895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="568" mass="62801" checksum="7896C80913A20157" modified="2024-05-29" version="1">MEDGELDFSTNEMFADANIGDIPSNDSLDSFFDEVFKDSHACTHTHTCNPPGPDSSHTHTCYHVHTKIVPAMSGDDDKTPTDDTAESADKKGKKRPLGNREAVRKYREKKKARAASLEDEVVRLRALNQQLMKRIQSQAGLEAEVARLKCLLVDIRGRIEGEIGSFPYQKQVPANHNIGGYVMNPCNMQCGDQVYCLHPEVDGKNVENASIHDPVINGCEFESLQCLGNQNSLLSQFPGCTTDDGILVANQSGGRAKRKGSRTAAEGLLLDVSRKGVVIGSWGGRVSKGNGGVDGVVMEDGELEFSNNEMFTDPNMADISSNDITNSFFDDVFMDSHVCTHTHICNPPGPDSSHTHTCYHVHTKLVPTISADDDTAESSDKKGKKQRPLGNREAVRKYREKKKARTASLEEEAVRLRAVNQQLMTRIQSQAGLEAEVLRLKCLLADIRGRIEGEIGSFPYQKQPPANHNIGNPNLTCGYVMNPCNMQCGDQVHCIHPEKTRRYMIQLSMVVKFENSQRLGNKILCSNEFQDCATQDGFLVPINNVYNVMLINNAFGDGIWAWAERTVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C0I8</accession>
    <name>A0AAD5C0I8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">2-phosphoglycerate kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_003647</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010197">
        <property type="protein sequence ID" value="KAI7732629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C0I8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33477:SF2">
        <property type="entry name" value="2-PHOSPHOGLYCERATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33477">
        <property type="entry name" value="P-LOOP NTPASE DOMAIN-CONTAINING PROTEIN LPA1 HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13238">
        <property type="entry name" value="AAA_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="246"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="11"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7732629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="450" mass="50401" checksum="11FBCF168EA7D38B" modified="2024-05-29" version="1">MKKEKSVSIASPSGTTSQPKSSSSSSSSNIPHHHHQQQEEEEEEEQSNKQQQQQKTPTIRTGTRNGSSKYDFVKIPNHVALKIALELKKLLIDNSLLDVSQSDLEANLFKLMERRGYGQEYINRYKMMTRFHHQRVPLVILVCGTACVGKSTIATQLAQRLNLPNVLQTDMVYELLRTSTDAPLTSSPIWARDFSSSEELITEFCRECRIVRKGLAGDLKKAMKDGKPIIIEGMHVDPSIYLMDEENKSPHNVPANSQESKTSKTDENEANNLENNASTFSAHGENIPQSDENSKTDVISTQELDKISDDLKSVHIVDHATNSQNDITKDQVADKKPTLEREKSGVTDPVIIPIVLKMADFDHKALLEEWISSRKFSDRYPIQEKDKLISNLKTIQDYLCSFKSQGLTVVNISTTTFPQTLDWLHNHLLQCIEQGITSASKGSNGHTGGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C1E9</accession>
    <name>A0AAD5C1E9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Kinesin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_024154</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Responsible for microtubule translocation. May be important for the organization of phragmoplast-specific arrays of microtubules. Plays an essential role in stabilizing the mitotic spindle. Required during mitotic cytokinesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Spindle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. KIN-5/BimC subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010205">
        <property type="protein sequence ID" value="KAI7732548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C1E9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005876">
        <property type="term" value="C:spindle microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008574">
        <property type="term" value="F:plus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090307">
        <property type="term" value="P:mitotic spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051231">
        <property type="term" value="P:spindle elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01364">
        <property type="entry name" value="KISc_BimC_Eg5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000019">
        <property type="entry name" value="Kinesin-like protein KIN-5D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047149">
        <property type="entry name" value="KIF11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047241">
        <property type="entry name" value="KIF11-like_kin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47970:SF12">
        <property type="entry name" value="KINESIN FAMILY MEMBER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47970">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIF11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6 10" id="KW-0505">Motor protein</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="13">
        <location>
            <begin position="10"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="981"/>
            <end position="1007"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="410"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="981"/>
            <end position="992"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="96"/>
            <end position="103"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00046159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7732548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="1007" mass="112698" checksum="3506C6DA15799C5D" modified="2024-05-29" version="1">MSARHEKGVNVQVLLRCRPFSNEELKNNAPQVVTCNEFQREVAVSQSIAGKQIDRVFTFDKVFGPTAQQKDLYEQAVIPIVNEVLEGFNCTIFAYGQTGTGKTYTMEGECKRAKSGPNGELPLEAGVIPRSVKQIFDTLEGQNAEYSVKVTFLELYNEEITDLLAPEEISKIGIEDKQKKLLPLMEDGKGGVLVRGLEEEIVTSASEIFTLLERGSAKRRTAETLLNKQSSRSHSLFSITIHIKESTPEGEELIKCGKLNLVDLAGSENISRSGAREGRAREAGEINKSLLTLGRVINALVEHLGHIPYRDSKLTRLLRDSLGGRTKTCIIATVSPAVHCLEETLSTLDYAHRAKNIKNKPEVNQKMMKTTLIKDLYGEIERLKAEVYASREKSGVYLPKERYYQEEMERKSMADQIEQMGIKIENQQKQFELLQAQFNARVEECSDLSCKLESTQNNLNETNNVLANTEETLKKCQYALKERDFIIQEQKKAENALAHQACVLRSDLEKSIQDNASLFMKIAREDKLSAGNRSVVNKFESELAQDVGSLCNMVAASVSQQNEQLQCIEKFCDTFISISDKAITDLKKKVSASRNLYISHIEEMENVVRLHKASANGSLEDISAQSSSNASSVEKLLAEEATKGHSIFDELHSTLSTQQGEIALFARELRKRFNDSIHHTTNISEFINGVFDKLMEESKEIGNHANQVNEIQTKNIDEFKKAYEEQSRSDAEKLIADVTSLVSSHLSKQKEMVDARLAGIRETCSGSKTVLDGHVSSIENITSDAKRKWQEFSLQAENNAKDSAEFSAAKHCRFELLLQKCVDATDMALNHSKKTHESVTDMGIKHVSAIEELVRTASESNEHHDAEISSARKTAEEDVMKNIEEIIKHIEGTSQEELEAISGILETTKAQAMNLETLQNDHSTQSGAIEQKAHDTFQQKYMDYEPTGNTPVRCDTDIPSKVAIESLRATPMETLMEEFRENNSAESLEIKKTKISHTPRSPLAEIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C3R7</accession>
    <name>A0AAD5C3R7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Thiaminase-2/PQQC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_027460</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009640">
        <property type="protein sequence ID" value="KAI7734823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C3R7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006772">
        <property type="term" value="P:thiamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.910.10">
        <property type="entry name" value="Heme oxygenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016084">
        <property type="entry name" value="Haem_Oase-like_multi-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004305">
        <property type="entry name" value="Thiaminase-2/PQQC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050967">
        <property type="entry name" value="Thiamine_Salvage_TenA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43198">
        <property type="entry name" value="BIFUNCTIONAL TH2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43198:SF2">
        <property type="entry name" value="SI:CH1073-67J19.1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03070">
        <property type="entry name" value="TENA_THI-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48613">
        <property type="entry name" value="Heme oxygenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thiaminase-2/PQQC" evidence="1">
        <location>
            <begin position="68"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7734823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="150" mass="17443" checksum="E995AFA68A37228B" modified="2024-05-29" version="1" fragment="single">MPSFFTKLSIQIRPTIRSAIFNTIPSNPFLKVKFLRVSSMAKPTEEEGTAKRFWDKFSKESVLTLYTPFLVSLASGNLKLDSFRHYVAQDVYFYKSFAQAYELAEDCADDDDAKVSIIELRKRVGHAIGKFSHRIPFYWLPLWVYRVTEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C410</accession>
    <name>A0AAD5C410_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_003000</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009653">
        <property type="protein sequence ID" value="KAI7734747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C410"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7734747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="187" mass="21175" checksum="A2065C8D008BE792" modified="2024-05-29" version="1" fragment="single">MASSLHWLPKLRNTIITPILLKRALTVSTSYTTTAVASNPDTSQTVSTLKSVRHEEYGRLLPCPSENTLPRNIEHLVVREEGPILDFIAKALDLPPLYVADLIHFGAVHYALVCPKPPLTATSDEIKLYKEFTKPSVLRKRPSIKGKTVREAQNTFRVTQPDQLVEAGTYVRVHVRPKRFPRYCKIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C564</accession>
    <name>A0AAD5C564_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-CH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_000520</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009505">
        <property type="protein sequence ID" value="KAI7735307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C564"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16495">
        <property type="entry name" value="RING_CH-C4HC3_MARCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46214:SF30">
        <property type="entry name" value="OS01G0850200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46214">
        <property type="entry name" value="ZINC FINGER, RING-CH-TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12906">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51292">
        <property type="entry name" value="ZF_RING_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="203"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="RING-CH-type" evidence="6">
        <location>
            <begin position="104"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="43"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="54"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="70"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7735307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="227" mass="25455" checksum="7DE01F0956714CC9" modified="2024-05-29" version="1" fragment="single">KPNSTSSSPSHCLSSSQFLLQQHSPLHQHMSTLHHPLPNDVVSPPPPPPPPPPNRRRRRRNRHRRRRPPTTENNILQSSVSVSSEEIDLESGELELEMEMKVHCLGKIEKQCRICHLGFDGDVIELGCDCKGDLGAAHKQCAETWFKIKGNMTCEICGATAQNVGGEQIIETSHTRSETEPIATGTMVIPTEPARTCWRGRRIMNILLGCMVFAFIISWLFHFNVLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C7C4</accession>
    <name>A0AAD5C7C4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">GDSL esterase/lipase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_009131</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009227">
        <property type="protein sequence ID" value="KAI7736666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C7C4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01837">
        <property type="entry name" value="SGNH_plant_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035669">
        <property type="entry name" value="SGNH_plant_lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22835:SF536">
        <property type="entry name" value="OS05G0401000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22835">
        <property type="entry name" value="ZINC FINGER FYVE DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7736666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="430" mass="47048" checksum="ECC332028929E731" modified="2024-05-29" version="1" fragment="single">TSKQTSLTNNSIPTLLQISPPKKPCLVTSVSYTTTTTTTTVTATATAATYQHDPLADMKYHGLLLIMFVITYVTNAIDFNYPAVFNFGDSNSDTGNLVAGIGEHLDPPNGQTYFKGPSGRFCDGRLIIDFLMDAMDLPFLNAYLEAIGVPNFRKGCNFAAAGSTIMPPGLNFVSPFSFGVQVAQFIRFKSHVLQLQAKYNKYIPDEDYFSKGLYMFDIGQNDLAGAFYSKSYDQVIASIPFVLIEFETGIKLLYDQGARNFWIHNTGPLGCLPQNIAKFGTDPTKLDAQGCVSAHNQAANLFNLQLHALVAKLQAQLSDANITHVDVFTIKSNLIANYSRYGFEQPTAACCGYGGPPLNYNSQLSCGQVKTINGSSITTTVCNDTTEYVSWDGIHYTEAANQHIASQILTGKFSDPPFADKMPFLLNLKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C8S8</accession>
    <name>A0AAD5C8S8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">M8C21_012312</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">AA19_3_7</strain>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">ATP-dependent RNA helicase involved in mRNA turnover, and more specifically in mRNA decapping.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">P-body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the DEAD box helicase family. DDX6/DHH1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010009090">
        <property type="protein sequence ID" value="KAI7737212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C8S8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd17940">
        <property type="entry name" value="DEADc_DDX6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000114">
        <property type="entry name" value="ATP-dependent RNA helicase DDX6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000364">
        <property type="entry name" value="ATP-dependent RNA helicase DDX6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47960">
        <property type="entry name" value="DEAD-BOX ATP-DEPENDENT RNA HELICASE 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 13" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="17">
        <location>
            <begin position="143"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="15">
        <location>
            <begin position="174"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="16">
        <location>
            <begin position="354"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="12">
        <location>
            <begin position="143"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="14">
        <location>
            <begin position="23"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="39"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAI7737212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="517" mass="58739" checksum="97F19F0621FE9D2D" modified="2024-05-29" version="1">MNNNNNSSVNDGNNRRYPPGIGNNRGGGGGWYGSGGGNNNNNIQTNPNYQHQPRNPNQIQQNQSQYVQRQQQYNQKQQWLKLNNPNNVSGSSTLNNNNNYEVEKAVQSEGIADSSSQDWKAQLNLPAADSRYKTEDVTATKGNEFEDYFLKRELLMGIYEKGFERPSPIQEESIPIALTGSDILARAKNGTGKTAAFCIPALEKIDTDNNVIQVVILVPTRELALQTSQVCKELGKHLKIQVMVTTGGTSLKDDIMRLYQPVHLLVGTPGRILDLTKKGICKLDNCTMLVMDEADKLLSPEFQPSVEQLISFLPENRQILMFSATFPVTVKDFKDRYLRKPYVVNLMDELTLKGITQYYAFVEERQKVHCLNTLFSKLQINQSIIFCNSVNRVELLAKKITELGYSCFYIHAKMLQDHRNRVFHDFRNGACRNLVCTDLFTRGIDIQAVNVVINFDFPRNAETYLHRVGRSGRFGHLGLAVNLITYEDRFNLYRIEQELGTEIKQIPPHIDQAIYCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C967</accession>
    <name>A0AAD5C967_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">40S ribosomal protein S19-3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_008840</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008996">
        <property type="protein sequence ID" value="KAI7737537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C967"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000118">
        <property type="entry name" value="40S ribosomal protein S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001266">
        <property type="entry name" value="Ribosomal_eS19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018277">
        <property type="entry name" value="Ribosomal_eS19_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11710">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11710:SF0">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01090">
        <property type="entry name" value="Ribosomal_S19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01413">
        <property type="entry name" value="Ribosomal_S19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00628">
        <property type="entry name" value="RIBOSOMAL_S19E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7737537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="143" mass="15877" checksum="3C7B608927BF3FDA" modified="2024-05-29" version="1">MEAARTVKDVSPHEFVKAYAAHLKRSGKMELPHWTDIVKTATFKELAPYDPDWYYIRAASMARKIYLRGGLGVGAFQRIYGGRKRNGSAPPHFCKSSGGVARHILQQLQTMNIVDFDSKGGRKITSNGRRDLDQVAGRVGVTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C975</accession>
    <name>A0AAD5C975_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Histone-lysine N-methyltransferase family member SUVH9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_003962</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008974">
        <property type="protein sequence ID" value="KAI7737658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C975"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042054">
        <property type="term" value="F:histone methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.280.10:FF:000003">
        <property type="entry name" value="Histone-lysine N-methyltransferase, H3 lysine-9 specific SUVH5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.280.10">
        <property type="entry name" value="SRA-YDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025794">
        <property type="entry name" value="H3-K9-MeTrfase_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051357">
        <property type="entry name" value="H3K9_HMTase_SUVAR3-9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007728">
        <property type="entry name" value="Pre-SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036987">
        <property type="entry name" value="SRA-YDG_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003105">
        <property type="entry name" value="SRA_YDG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45660:SF3">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE FAMILY MEMBER SUVH9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45660">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SETMAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05033">
        <property type="entry name" value="Pre-SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02182">
        <property type="entry name" value="SAD_SRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00468">
        <property type="entry name" value="PreSET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00466">
        <property type="entry name" value="SRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50867">
        <property type="entry name" value="PRE_SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51575">
        <property type="entry name" value="SAM_MT43_SUVAR39_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51015">
        <property type="entry name" value="YDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="2" id="KW-0158">Chromosome</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YDG" evidence="9">
        <location>
            <begin position="232"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="Pre-SET" evidence="8">
        <location>
            <begin position="458"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="domain" description="SET" evidence="7">
        <location>
            <begin position="519"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="63"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="72"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="679"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7737658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="679" mass="76386" checksum="E20FC35CE2ED8E26" modified="2024-05-29" version="1" fragment="single">ERVSLCATVSVSSPRISFFFLVYSKNKIKEKGMGSFIDLNLSPHPPNTTSTPTTLITPKIEPKLEPLDHQPPLHPPSPNFPPPNPNPNSNQLIPVDENNVYSEFNRISEMFRTAFANTTNTLQDFHATDPDAQALVPVPDDTQIATSLRRKDPPRSSELVRVTNLGLEDERYFREVMRKTRMIYDSLRVLVVAEDEKRKSFEFVRSPRGDLKAASVMKERGLWLNRDKRIVGAIPGVHVGDVFFFRMELCVVGMHGQAQAGIDYLTSTQSSNGEPIATSVIVSGGYEDDEDAGDVIIYTGHGGQDKHSRQVLHQKLEGGNLAMERSMHYGIEVRVIRGFRYEGSASGKVYVYDGLYKIIEAWFDVGKSGFGVFKFKLVRMEGQIDMGSSVLKFAENLRTKPLEVRPTGYVSFDISMKKETIPVFLFNDIDNNHEPMYYDYAATTVFPPFVYHLGGKDGGCNCVSGCSQDCFCAKKNGGDFAYDSNGLLVRGKPLIFECGPHCHCPASCQNRVSQKGVRNRFEVFRSRETGWGVRSLDLIQAGSFICEYTGVVLTREQAQLFAMNGDTLVYPNRFGERWAEWGDLSQIFSDYVRPSYPSVPPLDFGMDVSRMRNLACYMSHSSCPNVLVQLVLYDHSNIAFPHLMLFAMENIPPLRELSLDYGAADEWMGKFWSRMHDAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CA45</accession>
    <name>A0AAD5CA45_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_027838</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008972">
        <property type="protein sequence ID" value="KAI7737669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CA45"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000529">
        <property type="entry name" value="UBP1-associated protein 2C isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050886">
        <property type="entry name" value="RNA-binding_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48024">
        <property type="entry name" value="GEO13361P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48024:SF25">
        <property type="entry name" value="UBP1-ASSOCIATED PROTEIN 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="22"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="110"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="186"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="304"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="193"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="252"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="304"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="314"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="325"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="336"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7737669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="357" mass="36910" checksum="073BA7164EEB4AF0" modified="2024-05-29" version="1">MLRDVAVLDAVRSIADSDPTQRKLFIRGIGWETTTEKLRSVFSAYGELDEAVVITDKTTGKSKGYGFVTFKHIDGAIMALKDPSKKIDGRITVAQLAAARDSGNVDVSMRKIYVGNVPFEISSERLLSHFSSYGEIEEGPLGFDKQSGKTKGFAFFVYKTEEGARNSLVDPIKNIDGNQVMCKMATDGKKGKVGGPQGLPGMGGMPGDAPPPMPGSMNTGYGGLPPYGGYSSGRPMPHQNPPMNSSVPPAAAGGGGQGYGNQGPPSYGSGGYGGGGYNSGGYSSGSHYGGGAGSGDYPGVNSYGSSMSRMPPSSGGGYPDGGSYGVSSGYPTQMHQPPPPGPRGPPGPMYHGGPPYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CBN7</accession>
    <name>A0AAD5CBN7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_021528</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008818">
        <property type="protein sequence ID" value="KAI7738398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CBN7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7738398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="76" mass="8372" checksum="825A2246AB02FA2E" modified="2024-05-29" version="1" fragment="single">REKGRIWNRPWPPASHTNPAETLGIVDVDGPTREDLAIILEDLGINVDVVLKPNNIDIAPDFGRIGLQHRVAQIAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CE42</accession>
    <name>A0AAD5CE42_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Gamma-soluble NSF attachment protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">N-ethylmaleimide-sensitive factor attachment protein gamma</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_031820</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNAP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008695">
        <property type="protein sequence ID" value="KAI7738934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CE42"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005483">
        <property type="term" value="F:soluble NSF attachment protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000744">
        <property type="entry name" value="NSF_attach"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13768:SF2">
        <property type="entry name" value="GAMMA-SOLUBLE NSF ATTACHMENT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13768">
        <property type="entry name" value="SOLUBLE NSF ATTACHMENT PROTEIN SNAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14938">
        <property type="entry name" value="SNAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00042485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7738934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="268" mass="29935" checksum="6888D7B5EE3DD751" modified="2024-05-29" version="1">MATPDPEKLIAKADKLTKLTLTRWSADWKNATQYYEQAVKPWDAAKHMESAAALAVELGIWSEVADFYRRASELYNECGRSQPASDALAKGARALEDAKPEDAIAMYTDACSLLEEDGKENMAFDLYRAITNVYIKLEKYTDAASFLLRWAVAANKCNALHSQCKAYLSAIIVYLYAHDFKQAEQCYNDCCQIDTFLSSDQSHCASKLLSAYREADAEEIKRVAKSSTISNLDHMIIRLARKLPTGELVAMEVNSLDNGEALDEDDLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CFR4</accession>
    <name>A0AAD5CFR4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AT3G52170-like helix-turn-helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_000695</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008597">
        <property type="protein sequence ID" value="KAI7739626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CFR4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058942">
        <property type="entry name" value="AT3G52170-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058941">
        <property type="entry name" value="HTH_AT3G52170-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34568:SF4">
        <property type="entry name" value="OS02G0638000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34568">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25896">
        <property type="entry name" value="HTH_AT3G52170"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AT3G52170-like helix-turn-helix" evidence="2">
        <location>
            <begin position="64"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="167"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="168"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="188"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7739626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="262" mass="29527" checksum="C80D681F4407A770" modified="2024-05-29" version="1">MKSVGFLMPIVKGFRRFHSNILSSRQAATEISNLVSGSNVEQSGRASAASLTSNRPIRKRINIAERRERLESFVNKYRAMNLGKFPSPTTAKKEVGGGYYSVKKMLQEMEYNFKMSSVEKGPTTESLNHVEKGPTTENLNHVEKGLNMENINQVKDDDFLSQEIAMASSASQEKSNTDSGNNNITSDDTQKIATESDEKPEQNPRDDNFALKDPEPQVEKQPDGIRNVMRDKGEEDNELREKASLWGNLKSMANEFINMWKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CGB4</accession>
    <name>A0AAD5CGB4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein prune homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_022147</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008442">
        <property type="protein sequence ID" value="KAI7740405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CGB4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004309">
        <property type="term" value="F:exopolyphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.310.20">
        <property type="entry name" value="DHHA2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1640.10">
        <property type="entry name" value="inorganic pyrophosphatase (n-terminal core)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038763">
        <property type="entry name" value="DHH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038222">
        <property type="entry name" value="DHHA2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12112">
        <property type="entry name" value="BNIP - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12112:SF52">
        <property type="entry name" value="DHHA2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64182">
        <property type="entry name" value="DHH phosphoesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7740405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="495" mass="55191" checksum="144C833BCC574F37" modified="2024-05-29" version="1">MGRFCIYDSFMGSPSDSMLQNQQGKYIRARENPFFDGLKRGNASMNNRKSLFDFMGRTSKQKVTRSSSAIGHTSDLPQLHIRKEPFDMSLSGSVDLSSPQIADDIQSHRDVQKSFISFSKREQVSLPPCAASFYYGSSPQIDAVESCESIQKLNMYLKASKAAVNVGVPGQFLRVVLGPISDAGSFISIIMYSLYLHETDKTSQFCTVPVINLKRTDINSHGELDWLLSSCNIDLSSILFIDEVDLSYYDLFGSLKIVLLNADMLQEKHVALKDSVVEAFNCQKEGSCCALIAEKFASISPGILTGKGFSRLLLAGILLDTENLTSSRCTCVDRYMSTLLLNGAVRSKMHDVSQFSVGQILQKDFRKWSTIGKTDIDGPRLVVGMSAVGISISQLLSHNETSVQEIILFQQLEKLRLLIIISSYNHHKNFKEMLISAESEDLMKDLLHFLDTHATHLPLHALYHPGLGDEMRAFTIDSVPSRTTMEQLLQDFCNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CGP0</accession>
    <name>A0AAD5CGP0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RRP12-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_031108</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RRP12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008490">
        <property type="protein sequence ID" value="KAI7740194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CGP0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012978">
        <property type="entry name" value="HEAT_RRP12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057860">
        <property type="entry name" value="HEAT_RRP12_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48412">
        <property type="entry name" value="ARM REPEAT SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48412:SF1">
        <property type="entry name" value="ARM REPEAT SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25772">
        <property type="entry name" value="HEAT_RRP12_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08161">
        <property type="entry name" value="RRP12_HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RRP12 N-terminal HEAT" evidence="4">
        <location>
            <begin position="3"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="RRP12 N-terminal HEAT" evidence="4">
        <location>
            <begin position="96"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="RRP12 HEAT" evidence="3">
        <location>
            <begin position="229"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="826"/>
            <end position="947"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="826"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="862"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="898"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="932"/>
            <end position="947"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="947"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7740194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="947" mass="104792" checksum="951EFD38AE5D4CF3" modified="2024-05-29" version="1" fragment="single">KAVAKDKAAEAAEIVVKMMERECEGLPVSSVRALVKCLGVLVEFCELEDWDEVRFGFGMLFKYSVDKRPKDIKENELSCISQVVKTVDSRSVDGSKDENISKNEHLEVLHMLNLLKFLVPCLPSKVISKVVIELQMAISASFSALTRHVFDVMDEILRFLEAGSTIPDTEKIVTILSTYISKKQNPVDTIFSAAALLDTFLTKFQIGDPTKWNDHYSLIIGSIAGLLTSESTAMKASNILKEMINHHIDVEILLSSETMLADENNVESKESRLLKSLCDALLKVISAHKGIPNEHILAVISALFLKLGKSSHIYMGSVLLKLAVFMTATSGNTSDVKHLQECMGSAVIAMGPEKLLDILPISLVANDQTCSNSWLIPILKEYVAGSSLEFFIENVVPLAGSFQEESQNVKKSVSGEELQAYASGCWGLLPAFCRYPSDTHKKFQSLERLLIPCIRKDAFMLEDIAIAIQLLVKQNRSFLGAEQCDDEALKFPKLSYSKKAATKNIKALASCSEELLKAFTKVFFKVSKKKRVFVKDAIKCLTLITEPATIKKIFILSLKRLEQKVSADKVNAKRCLILDLASSVVGAASLDLIELIYNFIKQSLQEEDENIQREAYATLHKILEVHVELETTLDGENTCGFRMLNEVIVTLKDGKEESRKVAYDVLLGISSTLQKTDSTLEKGPYYEFVSMIMGYLSGSSPHIKSGAVSALSLVVYNDSKICTLMPDLVPSILELLHSKAIEVIKAVLGFLKVLVLSLQVSELQNFLSDILSGLLPWSSVSRHHFKSKVTVILEIMMRKCGSASVKSLVPAKYLDFVKDVLENRQGKTNSQETATTGTDAGLSETTTNSKPKFKSKSAGSFTKKDHSTETRKRKRDDKNRPDKPSKFSPRGNNNNTTKDVKHSGFAKSSDRPSKGGFKRKNPSFKNDTSGGKRQKQWTKGSKKNASG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CGZ3</accession>
    <name>A0AAD5CGZ3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Gelsolin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_026521</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008144">
        <property type="protein sequence ID" value="KAI7741721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CGZ3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051014">
        <property type="term" value="P:actin filament severing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007123">
        <property type="entry name" value="Gelsolin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007122">
        <property type="entry name" value="Villin/Gelsolin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11977">
        <property type="entry name" value="VILLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11977:SF107">
        <property type="entry name" value="VILLIN HEADPIECE, VILLIN_GELSOLIN, ADF-H_GELSOLIN-LIKE DOMAIN PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00626">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gelsolin-like" evidence="1">
        <location>
            <begin position="81"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7741721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="184" mass="20709" checksum="2BE826C4DACF8DC7" modified="2024-05-29" version="1">MSMVIRETQLDIVALDQSLLLGFWYTSFLKNRLQNGDESMQEIWLGLKPKSLDFVQVTAPPLAIETAYGGLERVSSGEGKFEVEEVNKFDQDDLLPVDISILDTHVEVFVWIGQAVDPKEKKSTLDYGQVNHREKFGACECGKWEGGLELSELVKEYDGFEGALVWDALKPDGTPRKLRITQPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CH89</accession>
    <name>A0AAD5CH89_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_005069</name>
        <name evidence="2" type="ORF">M8C21_005070</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008122">
        <property type="protein sequence ID" value="KAI7741871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAMZMK010008122">
        <property type="protein sequence ID" value="KAI7741872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CH89"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7741871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7741872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="43" mass="4872" checksum="31CCC6470EB4A536" modified="2024-05-29" version="1">MVDEEAVRVAGMPWLLLLLYLNAYSPDIKCYCSFPNSLATSLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CHP1</accession>
    <name>A0AAD5CHP1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_025445</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008351">
        <property type="protein sequence ID" value="KAI7740734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CHP1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7740734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="37" mass="3818" checksum="97B15D0505A84839" modified="2024-05-29" version="1">MVRFVSLTVENVFLTLLARISAGVSPQVAASIAEAGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CHV4</accession>
    <name>A0AAD5CHV4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">PROP1-like PPR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_020144</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008341">
        <property type="protein sequence ID" value="KAI7740824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CHV4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000394">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033443">
        <property type="entry name" value="PROP1-like_PPR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45717:SF15">
        <property type="entry name" value="AGL218WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45717">
        <property type="entry name" value="OS12G0527900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17177">
        <property type="entry name" value="PPR_long"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="PROP1-like PPR" evidence="8">
        <location>
            <begin position="227"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="PROP1-like PPR" evidence="8">
        <location>
            <begin position="402"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="6">
        <location>
            <begin position="429"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="6">
        <location>
            <begin position="500"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="80"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="81"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="92"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7740824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="591" mass="66980" checksum="07F0C9115757B674" modified="2024-05-29" version="1">MWAIRRASVRIRSQPLPSITIHACDAKFDTSSSYTGNIGFGGPSPPISDGSLLLRNFSFQARGSQSLFIGYHSFSSQAGSESEKELIKEADMSESESESENELGLLDTEEGIAETKGRERKGLSGLCKVVVESSSAPSNVKNALDKWVEEGHELSRSEISHAMFELRRRRMYGKALQLSEWLESQESIEISERDYASRVDLIAKVRGMQKAESYIEKIPESFKGEIVYRTLLANCVLNNDTKKAENVFNKMKDLNLPVTVFACNQLLLLYKRTDKKKIADVLLLMEKENVKPSLFTYRLLIETKGMVHDITGMEQVLETMKAEGLEPDLRLQSALARQYVYAGMNEKARDVLKEMEGSNLNENRGVCSSLLQIYASLGSVDDVKRVWEACNNPRLEECMNAIEAFGKLKKVEEAEAVFDLMTKKWNRLSAKYYTSMLKVYANNKMLVKGKEIVKQMSESGCRLGPWTWDALVKLYIESGEIEKADSILNKASKQLSMKPLFSTYMLLLDQYAKKGDVHNAEKIFYKMRQVGYVSRYRQYDSLLQAYINAKAPVYGFRERLRADNIFPSKTLAERLVLVDAFKKTAVSDLLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CHZ8</accession>
    <name>A0AAD5CHZ8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_024771</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the PP2C family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="4"/>
    <dbReference type="EMBL" id="JAMZMK010008036">
        <property type="protein sequence ID" value="KAI7742262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CHZ8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009738">
        <property type="term" value="P:abscisic acid-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000044">
        <property type="entry name" value="probable protein phosphatase 2C 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000222">
        <property type="entry name" value="PP2C_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00331">
        <property type="entry name" value="PP2C_SIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01032">
        <property type="entry name" value="PPM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 12" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="13">
        <location>
            <begin position="96"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7742262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="352" mass="38659" checksum="85A35F20DFEC2B6A" modified="2024-05-29" version="1">MCTVAILNSPVFSPESCSSSPSFKFRLQKPSVTGIFRASNDDVSNCLSNSPTQLKRKRPTRLNIPIAPLSFSADQLVTVETAVEDQWKEVEVEEEEYSVYCKRGRREKMEDRFKADVDFSGKNKVAFFGVFDGHGGSKAAEYASENLQKNIRDEVEKRGEMEIAEAIKQGYLNTDSEFLKEDQRGGACCVTAIISSGNLVVSNAGDCRAVVSRGGDAEALTSDHRPSRSDEKERIESLGGYVDCSRGVSRVLGSLAVSRGIGDRSLKQWITAEPETKMFKIIPEFEFLIMASDGLWEKVTNQEAVDLARPFCASGDKSETISACKKLVELSTSRGSIDDTSVMIVQLGCFCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CKJ5</accession>
    <name>A0AAD5CKJ5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_014291</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007681">
        <property type="protein sequence ID" value="KAI7743778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CKJ5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052799">
        <property type="entry name" value="Rho_GAP_Regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46265">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46265:SF2">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7743778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="54" mass="5847" checksum="9ACA976031EEF930" modified="2024-05-29" version="1" fragment="single">MEEEMSAIPQRGGEVNLTLGGIDLNNSGSVVVREDKKLLTVLFPDGRDGRAFTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-06-18" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CMP5</accession>
    <name>A0AAD5CMP5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Prolyl endopeptidase</fullName>
            <ecNumber evidence="6">3.4.21.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_032516</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of Pro-|-Xaa &gt;&gt; Ala-|-Xaa in oligopeptides.</text>
            <dbReference type="EC" id="3.4.21.26"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the peptidase S9A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.-" evidence="6"/>
    <dbReference type="EMBL" id="JAMZMK010007743">
        <property type="protein sequence ID" value="KAI7743331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070012">
        <property type="term" value="F:oligopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000005">
        <property type="entry name" value="Prolyl endopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.120">
        <property type="entry name" value="Prolyl oligopeptidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023302">
        <property type="entry name" value="Pept_S9A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002470">
        <property type="entry name" value="Peptidase_S9A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051167">
        <property type="entry name" value="Prolyl_oligopep/macrocyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42881">
        <property type="entry name" value="PROLYL ENDOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42881:SF2">
        <property type="entry name" value="PROLYL ENDOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02897">
        <property type="entry name" value="Peptidase_S9_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00862">
        <property type="entry name" value="PROLIGOPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50993">
        <property type="entry name" value="Peptidase/esterase 'gauge' domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 6" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Peptidase S9A N-terminal" evidence="8">
        <location>
            <begin position="14"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S9A N-terminal" evidence="8">
        <location>
            <begin position="227"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="7">
        <location>
            <begin position="455"/>
            <end position="684"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU368024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7743331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="689" mass="77312" checksum="6AB3535963986CC4" modified="2024-05-29" version="1">MGSLSLSDVPLQYPSFRRDESVVDNYHGVPVPDPYRWLEDPDAEEVKEFVKKQVELTDSVLKKCETREKLHEKLTKFYDYPKYGAPFREGDKYFYFHNTGLQPQKVLYMQDSLDGEAEVLLDPNGLSEDGTVALSTYAVSEDAKYLAYALXXXXXXXXXXXXXXXXXXSLIRCHGLNFRVLVGQMTVKVSSTAVIQLQIVNKGRLCCIIWSQVLAQSGKEGENLDAGTETNANLDHQLYYHFLGTDQSEDILCWETPDNPQYTLGASVTEDGKFVLLYINEGCDPVNKFYYCDLSTLPGGIKGCKENKRLPFIKLIDNFEAMYHAIANDDTVFTFLTNKNAPRYKLVRVDLKEPNTWTEVIPQSESDVLDTAVAVNGSQIVCDLESGVPDLKIFREIVVPGFDQTEFHVTQVFVPSKDGTKIPMFVVAKKDIVLDGSHPCLLYGYGGFNISLTPYFSVSRTVLMRHLGLVFCLANIRGGGEYGEEWHKDGSLAKKQNCFDDFISCGEYLISLGYTQSKKLAIEGGSNGGTLVGACNNQRPDLFGCVLAHVGVMDMLRFHKFTIGHAWTSDYGCSDKEEEFQWLIKYSPLHNVRRPWEQSNDISRQYPPTLLLTADHDDRVVPLHTLKLLATMQYVLCTSLENSPQTNPILGRIDCKAGHGAGRPTQKVIDEWADSYSFMAKAVGATWID</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CPD7</accession>
    <name>A0AAD5CPD7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Serine aminopeptidase S33 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_020474</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007134">
        <property type="protein sequence ID" value="KAI7745788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CPD7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051044">
        <property type="entry name" value="MAG_DAG_Lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11614">
        <property type="entry name" value="PHOSPHOLIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine aminopeptidase S33" evidence="1">
        <location>
            <begin position="53"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7745788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="314" mass="35523" checksum="C8E1B5CB886B026D" modified="2024-05-29" version="1">MEETQTKNRKYYWGNTPEEQDYYKLHNITSTSSIFTSPRGFSLFTRSWLPLSPPRGIICMVHGYGNDISWTFQATAIFLASNGFACFAADMEGHGKSQGLKAFVPNVNDVVEDYLSFFTSLLDSNDYYYKVPKFLFGESMGGAICLLIQFSKRLFDGAVLVAPMCRISDKVRPKWPIPEILMFVSRFAPTLAIVPTADLVDKSVKVAEKRLIGAMNPMRYTGKPRLGTVMELLRVTDYLSGRLIEVELPFIVLHGDADAVTDPEVSKELYEKAKSEDKRLKIYEGMMHSLLFGETDENVEVVRGDILAWLNERC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CQX7</accession>
    <name>A0AAD5CQX7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_027092</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007190">
        <property type="protein sequence ID" value="KAI7745605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CQX7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7745605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="108" mass="12185" checksum="E1E215B5246A2AC3" modified="2024-05-29" version="1" fragment="single">MQVNEYEKIKQLNVLENQKRLEELGIKSILNSMTSLAQSQKAKRKTLKQNTNVGDVECVVDVGNATKKQRTPRYIAPMSTNGVANLSRLRRVMAPSEGNKGRAKRRMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CSU6</accession>
    <name>A0AAD5CSU6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ARM repeat superfamily protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_023939</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006814">
        <property type="protein sequence ID" value="KAI7747224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CSU6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47673">
        <property type="entry name" value="ARM REPEAT SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47673:SF1">
        <property type="entry name" value="ARM REPEAT SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7747224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="195" mass="21917" checksum="575F64AC83DFDE4C" modified="2024-05-29" version="1">MRILAAQLSSYLCRRRPVNVRSRQYSSSNYERGFPDEREMEEEAERKIGWLFKLIFAGTATIIGYNIFPYLGDNLIQQSVSLLNVKDPLFKRMGASRLARFATDDERRMKIVEMGGATQLVEMLGAANDEATRKEALNAIIAIARSDEAVGALHGAGAISVIKATPQSTEDAEIEKYKAKLLKRFKDMKYDENSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CU02</accession>
    <name>A0AAD5CU02_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_031524</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006576">
        <property type="protein sequence ID" value="KAI7748238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CU02"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051106">
        <property type="entry name" value="RNA-bind/splicing_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48028">
        <property type="entry name" value="GLYCINE-RICH RNA-BINDING PROTEIN RZ1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48028:SF4">
        <property type="entry name" value="SC35-LIKE SPLICING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="16"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="86"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="86"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="107"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="137"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="150"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7748238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="203" mass="22957" checksum="9ACF3A93E7445F9B" modified="2024-05-29" version="1">MSHFGRAGPPDIRDTYSLLVLNITFRTTADDLFPLFDKYGKVVDVFIPRDRRTGDSRGFAFVRYKYADEAQKAFAKYGPDAERIHKGRIVEPVDKLKGRSRSRSPRPRSVSPPARGSVSPRKSPSPRRTPSRSRSPAARDEKERSPTPKSVSPRGRSASRSPSPQRSDADLLYAVGENRCSLQQPLSCWVDVFSWWLNPILRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CV03</accession>
    <name>A0AAD5CV03_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">tRNA (guanine(37)-N1)-methyltransferase</fullName>
            <ecNumber evidence="10">2.1.1.228</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">M1G-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">tRNA [GM37] methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">tRNA methyltransferase 5 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_007566</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>guanosine(37) in tRNA + S-adenosyl-L-methionine = N(1)-methylguanosine(37) in tRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36899"/>
            <dbReference type="Rhea" id="RHEA-COMP:10145"/>
            <dbReference type="Rhea" id="RHEA-COMP:10147"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73542"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="EC" id="2.1.1.228"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="10">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Mitochondrion matrix</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="10">Predominantly in the mitochondria and in the nucleus.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the TRM5 / TYW2 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM5/TYW2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.228" evidence="10"/>
    <dbReference type="EMBL" id="JAMZMK010006526">
        <property type="protein sequence ID" value="KAI7748409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CV03"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052906">
        <property type="term" value="F:tRNA (guanine(37)-N1)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070901">
        <property type="term" value="P:mitochondrial tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002939">
        <property type="term" value="P:tRNA N1-guanine methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.110:FF:000001">
        <property type="entry name" value="tRNA (guanine(37)-N1)-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000225">
        <property type="entry name" value="tRNA (guanine(37)-N1)-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.110">
        <property type="entry name" value="Met-10+ protein-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03152">
        <property type="entry name" value="TRM5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030382">
        <property type="entry name" value="MeTrfase_TRM5/TYW2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056743">
        <property type="entry name" value="TRM5-TYW2-like_MTfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056744">
        <property type="entry name" value="TRM5/TYW2-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025792">
        <property type="entry name" value="tRNA_Gua_MeTrfase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23245:SF43">
        <property type="entry name" value="TRNA (GUANINE(37)-N1)-METHYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23245">
        <property type="entry name" value="TRNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02475">
        <property type="entry name" value="TRM5-TYW2_MTfase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25133">
        <property type="entry name" value="TYW2_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51684">
        <property type="entry name" value="SAM_MT_TRM5_TYW2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 10" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 10" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="SAM-dependent methyltransferase TRM5/TYW2-type" evidence="11">
        <location>
            <begin position="117"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="245"/>
            <end position="246"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="273"/>
            <end position="274"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="296"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7748409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="390" mass="44497" checksum="C9EDA88BD2D0402F" modified="2024-05-29" version="1">METERGEVRDGKGNGCYLVAEVVGEEREGEEEDLSRLIGDTAGYRLGKWKGSTRLLLLDERYADKLYDELPEAVKACLKGSVFELVRCKLTLYYSYWQMNEVLEALLPEHMIVPSSFETVGHIIHLNLRDEHLPYKNLIAKVVLDKNKPKIQTVVNKIDAINNDFRTMQLEVLAGNHSLVTRLVENGLHFHVDLASVYWSSKLSTERQRLLTCFTRSDVVCDVFAGVGPLAIFAAKKVKYVYANDLNPHAVDYLERNCVLNKLERKIEVFNMDGRRFIDAIFKSQSTRPITQVVMNLPKDAAEYLDAFKGIFRDVERSEGLTLPTIHVYGFSKAEDPEFDFHERIRIALSEVAFEVKMHKVRLVAPGKWMLCASFVLPERVALAKPISEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CWA7</accession>
    <name>A0AAD5CWA7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">E2 ubiquitin-conjugating enzyme</fullName>
            <ecNumber evidence="1">2.3.2.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_029127</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="1"/>
    <dbReference type="EMBL" id="JAMZMK010006360">
        <property type="protein sequence ID" value="KAI7749446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CWA7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd23837">
        <property type="entry name" value="UBCc_UBE2O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000028">
        <property type="entry name" value="Probable ubiquitin-conjugating enzyme E2 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057733">
        <property type="entry name" value="UBE2O-like_SH3-B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057734">
        <property type="entry name" value="UBE2O-like_SH3-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057735">
        <property type="entry name" value="UBE2O-like_tSH3-B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46116">
        <property type="entry name" value="(E3-INDEPENDENT) E2 UBIQUITIN-CONJUGATING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46116:SF15">
        <property type="entry name" value="(E3-INDEPENDENT) E2 UBIQUITIN-CONJUGATING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23043">
        <property type="entry name" value="SH3-B_UBE2O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23044">
        <property type="entry name" value="SH3-C_UBE2O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23046">
        <property type="entry name" value="tSH3-B_UBE2O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="7">
        <location>
            <begin position="587"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="437"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="520"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7749446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="837" mass="93188" checksum="516DFBA08EFACB64" modified="2024-05-29" version="1">MDVFLSDFDSYSESSSSDDQEDDEFMYSGQATSILSNLQETIGKIDDFLLFERRYVHGDIVYSGVDRDSQMGKVTNVEIVVDLENLNEKKVKDISSKKLGRIRSISTGEFVISGPWVGKAENITDSVTVLFDDGTKCEFTVTGLETEKLLPISPDLIDDSQYPYYPGQRVKAVNLTSSKSNRWFCGSTNKNQDEGTVCSVDSGLVHVNWLGCVLLGSESIPVPSSLQSAKDLIPVSCFSHSNWQIGDWCTLPKAEHKSFTDHLVHKQSKICSDFQEMFVIAKMKCKVDVLWQDGSESCGLCSTSLGPINTLDAHDFWPHQFVLEKGTSDDQQDRKWGVVKAVDPNEKTVKVKWESEIEETVSAYELIEHPDYSYNQGDLVLRLNKDQLVEDVEKCNSDLYLAHIGIVIGLKHGLVEVKWAAGFTSKVAPHEVLRVEKSEGPSATPLLNNGSIQDDQEKSGLSPDPRDKSFSGSDDGDDCLKTLYESGTFNVPRVSIGLLSDVVKSLFGFPFYASLSDAKDHVPENGSKSGTDEEEDPVYVETSSKVNCKDVEELNEIKQFDMVNDCSDHHFVDSPGKSSISSQVKKCWLKKVQQEWSILENNLPETIYVRVFEERMDLIRAAVVGAPGTPYHDCLFFFDIFLPPEYPHEPPMVHYDSGGLRVNPNLYESGRVCLSLLNTWTGTGSETWNPNSSTILQVLLSLQALVLNKNPYFNEAGYDQQIGSPEGEKNSGSYNENAFLMSCKSMLYVLRKPPKHFEALVEEHFSKRCVHILMACKAYLEGVPVGCGFGSPYLEPEGHGHTGNSTGFKIMLSKLVPKLVEAFTAKGFDCGEFSETR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CWQ0</accession>
    <name>A0AAD5CWQ0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Quinolinate synthase, chloroplastic</fullName>
            <ecNumber evidence="4">2.5.1.72</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">M8C21_024939</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">AA19_3_7</strain>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Involved in the biosynthesis of pyridine alkaloid natural products, leading mainly to the production of anabasine, anatabine, nicotine and nornicotine, effective deterrents against herbivores with antiparasitic and pesticide properties (neurotoxins); nornicotine serves as the precursor in the synthesis of the carcinogen compound N'-nitrosonornicotine (NNN). Catalyzes the condensation of iminoaspartate with dihydroxyacetone phosphate to form quinolinate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>iminosuccinate + dihydroxyacetone phosphate = quinolinate + phosphate + 2 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25888"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29959"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
            <dbReference type="EC" id="2.5.1.72"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; NAD(+) biosynthesis; quinolinate from iminoaspartate: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Plastid</location>
            <location evidence="2">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the quinolinate synthase family. Type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.72" evidence="4"/>
    <dbReference type="EMBL" id="JAMZMK010006453">
        <property type="protein sequence ID" value="KAI7748852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CWQ0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008987">
        <property type="term" value="F:quinolinate synthetase A activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034628">
        <property type="term" value="P:'de novo' NAD+ biosynthetic process from L-aspartate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10800:FF:000008">
        <property type="entry name" value="Quinolinate synthase chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10800:FF:000006">
        <property type="entry name" value="Quinolinate synthase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1010.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10800">
        <property type="entry name" value="NadA-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003808">
        <property type="entry name" value="Fe-S_metab-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003473">
        <property type="entry name" value="NadA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036094">
        <property type="entry name" value="NadA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30573:SF0">
        <property type="entry name" value="QUINOLINATE SYNTHASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30573">
        <property type="entry name" value="QUINOLINATE SYNTHETASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02445">
        <property type="entry name" value="NadA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02657">
        <property type="entry name" value="SufE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142754">
        <property type="entry name" value="NadA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82649">
        <property type="entry name" value="SufE/NifU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="12" id="KW-0408">Iron</keyword>
    <keyword evidence="13" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0934">Plastid</keyword>
    <keyword evidence="8" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Fe-S metabolism associated" evidence="18">
        <location>
            <begin position="89"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00052166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00053152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00061471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00073351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF02657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAI7748852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="724" mass="78875" checksum="1D44712F7A7062C2" modified="2024-05-29" version="1">MDTATFSITTYSSPSFNTTKLPLSSFTLFKTFKPFNNNNNNNNNNPSTPIISISRNPRLTLSCSAVTVTPSVTSDGHISALSKLQTLTSEFNSIPDPIDRVKRLIHYANRLPELDGSVRVSSNRVMGCTAQVWLDVRMDGDGTVRFMVDSDSEVIKGFCSCLVWVLDGARPEEVLGMRMEDFEGLNVGGGLYGGKVESRVNSWYNVLVSMQKRTKALVAQREGKAVGEAFPSLVITADGVGAKGSFAEAQARFLFPDDAKVQELANLLKERQVGVVAHFYMDPEVQGVLTSAQKLWPHIHISDSLVMADSAVSMAKAGCKFIAVLGVDFMSENVRAILDQAGFPEVGVYRMSDEQIGCSLADAASSPAYTSYLSEASNSSPSLHVVYINTSLETKAHAHELVPTITCTSSNVVQTILQAFAEVPNLNVWYGPDSYMGANIVELFQQMTFMTDEEIAKIHPEHTRNSINSLLPRLHYFQDGTCIVHHLFGHEVVEKIKDMYCDAFLTAHFEVPGEMFSLAMEAKRRGMGVVGSTQNILDFIKSRVQEALDRNIDDHLQFVLGTESGMVTSIVAAVRQLLCSSESSSRTGKVSVEIVFPVSSDSVTRVSQKSSTGPKFDDGLNVSVIPGVASGEGCSLHGGCASCPYMKMNSLTSLIKVLTSLPHGKDGLTRYEAGRFSLLTPKGNLIADVGCEPILHMRHYQATKKLPEKLIYQILGANNNGKAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CZM1</accession>
    <name>A0AAD5CZM1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein ORANGE, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_023665</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006388">
        <property type="protein sequence ID" value="KAI7749330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CZM1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15852:SF24">
        <property type="entry name" value="OS02G0651300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15852">
        <property type="entry name" value="PLASTID TRANSCRIPTIONALLY ACTIVE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="157"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="209"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7749330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="317" mass="34480" checksum="0BCF0117BD765126" modified="2024-05-29" version="1">MVCSARILDVSYPLTPSFCSYVPPAHHNSRYLYRNLKSTVRWRLMSSSDQDASMYASASSIDADSAAKNAAGFCIIEGPETVQDFDKMDIQEIQDNIRSRRNKIFLHMEEVRRLRIQQRIKSAELGIVNDEQDSELPAFPSFIPFLPPLSSANLKQYYATCFSLISGIIIFGGLLAPTLELKLGIGGTSYADFISSMHLPMQLSDVDPIVASFSGGAVGVISALMVVEINNVKQQEHKRCKYCLGTGYLACARCASTGAIVLIDPVASANGGNQPLSPPKTERCSNCSGAGKVMCPTCLCTGMAMASEHDPRIDPFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-10-08" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D1N8</accession>
    <name>A0AAD5D1N8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Auxin response factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_029052</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Auxin response factors (ARFs) are transcriptional factors that bind specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs). Could act as transcriptional activator or repressor. Formation of heterodimers with Aux/IAA proteins may alter their ability to modulate early auxin response genes expression.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 10">Homodimers and heterodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the ARF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006166">
        <property type="protein sequence ID" value="KAI7750335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009734">
        <property type="term" value="P:auxin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007389">
        <property type="term" value="P:pattern specification process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048829">
        <property type="term" value="P:root cap development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd10017">
        <property type="entry name" value="B3_DNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.1040:FF:000002">
        <property type="entry name" value="Auxin response factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.330.10:FF:000001">
        <property type="entry name" value="Auxin response factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.330.10">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010525">
        <property type="entry name" value="ARF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044835">
        <property type="entry name" value="ARF_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003340">
        <property type="entry name" value="B3_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015300">
        <property type="entry name" value="DNA-bd_pseudobarrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053793">
        <property type="entry name" value="PB1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31384:SF39">
        <property type="entry name" value="AUXIN RESPONSE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31384">
        <property type="entry name" value="AUXIN RESPONSE FACTOR 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06507">
        <property type="entry name" value="ARF_AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02362">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01019">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101936">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50863">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51745">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0927">Auxin signaling pathway</keyword>
    <keyword evidence="5 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 10" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TF-B3" evidence="12">
        <location>
            <begin position="129"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="PB1" evidence="13">
        <location>
            <begin position="572"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="547"/>
            <end position="566"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7750335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="636" mass="70030" checksum="16260421AC5DE93F" modified="2024-05-29" version="1">MITFMDSKEKLQQTERCLDSQLWHACAGSMIQMPPVNTKVYYFPQGHAEHAADVVEFRDQTGRSRIPAWVPCRVGSVRFMADVDTDEVYARIRLIPVTGTGEFSSLEDEDTGLGSGSGSLEGQVKPTSFAKTLTQSDANNGGGFSVPRYCAETIFPRLDYSADPPVQTILAKDVHGDTWKFRHIYRGTPRRHLLTTGWSTFVNHKKLVAGDSIVFLRAENGDLCVGIRRAKRGIGAGQETSSGWNPGGGNCVGPYGGYSSFIRNGNNGGAGGNSMEKVKVEPDSVVQAVKQAMSGQAFEVVFYPRASTPEFCVKASLVKNAMQIRWSPGMRFKMPFETEDSSRISWFMGTISSVQVADPIRWPDSPWRLLQVTWDEPDLLQNVKRVNPWLVELVSTMPAIHMSPFSPPRKKCRPSQHPDYPMESQIPIPALCGPHSHLFGGPTTGPFGYQLPENLNLHHPASIQGARHTRYGLSLSDIQLNNKLNSGFYPTPMKHSHSSPSVNRPAQSEPVSCLLTIGNSTISSKEPETPKSSQFLLFGQPILTEQQISQRSGSGSGSGSGSGREMDLNLETGHCKVFMESEDVGRTLDLSLLDSYEELCRKLANMFKTENSIMLDRVXXXLGCNRNIKADWGGTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D3B8</accession>
    <name>A0AAD5D3B8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">FMN hydroxy acid dehydrogenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_015056</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005706">
        <property type="protein sequence ID" value="KAI7752310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D3B8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003973">
        <property type="term" value="F:(S)-2-hydroxy-acid oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000262">
        <property type="entry name" value="FMN-dep_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037396">
        <property type="entry name" value="FMN_HAD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10578:SF70">
        <property type="entry name" value="GLYCOLATE OXIDASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10578">
        <property type="entry name" value="S -2-HYDROXY-ACID OXIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01070">
        <property type="entry name" value="FMN_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51349">
        <property type="entry name" value="FMN_HYDROXY_ACID_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FMN hydroxy acid dehydrogenase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7752310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="103" mass="11552" checksum="26D09A40BD91C43F" modified="2024-05-29" version="1" fragment="single">MEVTNVTEYQAIAKEKLPKMVYDYYASGAEDQWTLEENRNAFSRILFRPRILIDVSKIDMTTTILGFKLSMPIMIAPTAMQKMAHPDGEYATARAASAAGTIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D4U1</accession>
    <name>A0AAD5D4U1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methyltransferase type 11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_009055</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010003880">
        <property type="protein sequence ID" value="KAI7754313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D4U1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009820">
        <property type="term" value="P:alkaloid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45180">
        <property type="entry name" value="OS01G0307686 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45180:SF1">
        <property type="entry name" value="OS01G0307686 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="1">
        <location>
            <begin position="39"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7754313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="268" mass="30646" checksum="5AA2514F98CFAAF8" modified="2024-05-29" version="1">MADLFLKQAKQYWKTRPGYPQQLFDFIASKTPTHDVVWDVGTGSGQAAVSLASIYKSVIGTDTSKNQLEFAPKLPNIRYECTPPNMSMSELEEKIGTESSIDLVTVAQALHWFDHDAFYNQVKWILKKPNGVFAAWCYTTPKIDDEFDRVFQKFYSESKPYWEAERGLVDDKYTGVKFPFDPVDGCDHTGPFEFETKQVMSLKELFTYVRSWSAYQTAKGKGVDLLNDDVIEEFRKAWKEDENGEKSVTYPVYLRIGKAGGMVEQQAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D553</accession>
    <name>A0AAD5D553_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_002637</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005794">
        <property type="protein sequence ID" value="KAI7751910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D553"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="92"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7751910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="136" mass="15976" checksum="E1A372C1FD11495D" modified="2024-05-29" version="1">MYSPRPPNIADQLFDYSLTASPMSIIPHTLSFGFSFNEQDDSVEHHARSDSYGDPNPHHSSVGLLNIYHMVLRTVITYILIWSYSCDERISFNYYYVWFCNSVTYILTFWQHGFVIHMPSCFSYACNRILERIVEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D5N1</accession>
    <name>A0AAD5D5N1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">glutathione-specific gamma-glutamylcyclotransferase</fullName>
            <ecNumber evidence="4">4.3.2.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_015862</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the gamma-glutamylcyclotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.7" evidence="4"/>
    <dbReference type="EMBL" id="JAMZMK010005665">
        <property type="protein sequence ID" value="KAI7752511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D5N1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061928">
        <property type="term" value="F:glutathione specific gamma-glutamylcyclotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006751">
        <property type="term" value="P:glutathione catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06661">
        <property type="entry name" value="GGCT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.490.10:FF:000002">
        <property type="entry name" value="Gamma-glutamylcyclotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.490.10">
        <property type="entry name" value="Gamma-glutamyl cyclotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006840">
        <property type="entry name" value="ChaC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013024">
        <property type="entry name" value="GGCT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036568">
        <property type="entry name" value="GGCT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12192">
        <property type="entry name" value="CATION TRANSPORT PROTEIN CHAC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12192:SF19">
        <property type="entry name" value="GAMMA-GLUTAMYLCYCLOTRANSFERASE 2-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04752">
        <property type="entry name" value="ChaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110857">
        <property type="entry name" value="Gamma-glutamyl cyclotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7752511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="225" mass="25581" checksum="B75C84F6CBC8C94F" modified="2024-05-29" version="1">MVFWVFGYGSLVWNPGFEYDEKIIGYIKNYRRVFDLACIDHRGTPEHPARTCTLEYKEGAICWGAVYCVRGGEEKERLALKYLENRECEYDQKTTIDFFKEGETDEPTLSGVIVFTSTPDKESNKYYLGPAPLEDMARQIATAFGPCGNNRDYIFLLEKAMFDIGHEDDMVIELANEVRKVLGIMGLGVRKETSTNRLTGPSLKPKSPMSPRKLLHLPEAIAMDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D845</accession>
    <name>A0AAD5D845_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_013438</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000569">
        <property type="protein sequence ID" value="KAI7756128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D845"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7756128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="82" mass="9258" checksum="7AFAC2D2F27F677A" modified="2024-05-29" version="1" fragment="single">IFKWHAPPKNKDVVIGHDCLIEQDSSDTIVFMIASRMELGTQKECIWNIIVGIGNVFHGKRSRSNVPCCTQTHMDAHLLLVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D9A5</accession>
    <name>A0AAD5D9A5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_029303</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000980">
        <property type="protein sequence ID" value="KAI7755597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D9A5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.1150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47162">
        <property type="entry name" value="OS02G0192300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47162:SF9">
        <property type="entry name" value="PHD FINGER PROTEIN EHD3-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="6">
        <location>
            <begin position="367"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="54"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="54"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="73"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="83"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7755597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="480" mass="52447" checksum="1E0425603D91139D" modified="2024-05-29" version="1">MTNLDEKMKTADDSAPDQQAQDNHILSFDAFTNGPGECSRRPCSNGNENGLSGCIQNTSVPNQESGFMPLSKKSVDDAERSDNQTNSSAPTPSHVTSSGLFKQSDGSTSPDSCLGALSDILKSDEFSEVCGLLLKNFGVVNVNQVLHIDSISSKMKNGAYETSPMLYLKDIQQVWTKLQQAGNEMVTLANNLSDISRAHYDQCFRKPPATEAGGCQGCGEQAEVSNCLVCDSCEDIYHLSCTELAGTEIPPCWYCASCVSNGAGSPHDNCVVCEKLKASASIPNKVSTNDQSQDVPNGVEAKVNNNLHNVNDENQSSNICFMCKSEVKIGDEYKKCGHSLCVHKFYHSKCLTSKQSGVCGSCWYCPSCLCRRCLVDKDDDQIVLCDGCDEAYHIYCTSPQLLSIPKGSWFCRKCDRKVKGINTMKRVYESLQKKVKVEDGSEKAEHKEANDEREGLDMLVTAAKTLSHPGAFWNTRSNIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DGK8</accession>
    <name>A0AAD5DGK8_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_014663</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000008">
        <property type="protein sequence ID" value="KAI7758301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DGK8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7758301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="112" mass="12589" checksum="B0277F6873A5037D" modified="2024-05-29" version="1">MENPAMCEVELTTAPSIEDGKHWGYSELNPSCAASFDKPVSRSDVDTLCIIRNDVQLECNKVEGGCTMPFIAWNHTSCYSGGLLTIIKTKRIWRRLFCPCLEWWLDADDGVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G136</accession>
    <name>A0AAD5G136_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_028990</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012208">
        <property type="protein sequence ID" value="KAI7724509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G136"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7724509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="74" mass="8658" checksum="304A11049DC37DD5" modified="2024-05-29" version="1">MSSKELKRPVGCKYEMDPPTTLTCDLRPCQKQALFWMTESEKAVDVEKAKETLHPCWAAYRICDEYDIQLFINL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G1L2</accession>
    <name>A0AAD5G1L2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_013901</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012039">
        <property type="protein sequence ID" value="KAI7725155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G1L2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7725155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="80" mass="8925" checksum="593EDC1C4B4C99C6" modified="2024-05-29" version="1" fragment="single">IRSSNILVRNHANAKIIVVAKNNKVKTSRVQTVNKNTINDLTILYLNITPTKHSVNPSVQTPSTRKLIGFNYNGNPRDLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-02-05" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G262</accession>
    <name>A0AAD5G262_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Magnesium transporter CorA-like family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_018568</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CorA metal ion transporter (MIT) (TC 1.A.35) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012125">
        <property type="protein sequence ID" value="KAI7724948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046873">
        <property type="term" value="F:metal ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.340">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045861">
        <property type="entry name" value="CorA_cytoplasmic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045863">
        <property type="entry name" value="CorA_TM1_TM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002523">
        <property type="entry name" value="MgTranspt_CorA/ZnTranspt_ZntB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46950">
        <property type="entry name" value="MAGNESIUM TRANSPORTER CORA-LIKE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46950:SF7">
        <property type="entry name" value="MG2+ TRANSPORTER PROTEIN, CORA-LIKE_ZINC TRANSPORT PROTEIN ZNTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01544">
        <property type="entry name" value="CorA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143865">
        <property type="entry name" value="CorA soluble domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144083">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="390"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="422"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7724948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="482" mass="54657" checksum="36B159F0EF2FDF94" modified="2024-05-29" version="1">MSNMKKFNNQKNQAGKDAMVDCAMWTDGLICAFEAKRSHKKCRPQDIIGWNKQGFKTGNDNLDDRVSDNIYTSCHWVPIGWSRINQLVETIQVDGSWEAEHTEYQINDNEVTVADIAAPYWERPGGPTWWCHVDAGHPSVNTWLRNSKWLHPAISIALRDESKLISERMKHLLYEVPVRVAGGLLFELLGQSAGDPYVEEDDIPVVLRSWQAQNFLVTALHVKGYAANINVLGVSEVQELLAAGGSSLPQSVHEVIALLACRLARWDDRLFRKYIFGAADEVELKFMNRRNHEDLHLFSIILNQEIRRLSAQVIRVKWSLHAREEIVFELLQHLRGNAAKRLLEGITKSTRQMIEEQEAVRGRLFTVQDVMQSTVRAWLQDKSLRVTHNLGVFGGCGLVLSIITGLFGINVDGIPGSENSPYAFLLFSCLLVLLGIILIFIGLLYLGLKNPTMEEEVEVRKLELQEIVKMFQQEAESXXXKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G2N5</accession>
    <name>A0AAD5G2N5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">40S ribosomal protein S30</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_010937</name>
        <name evidence="6" type="ORF">M8C21_010945</name>
        <name evidence="8" type="ORF">M8C21_025852</name>
        <name evidence="7" type="ORF">M8C21_026106</name>
    </gene>
    <organism evidence="6 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the eukaryotic ribosomal protein eS30 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011838">
        <property type="protein sequence ID" value="KAI7725869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAMZMK010011838">
        <property type="protein sequence ID" value="KAI7725877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAMZMK010006604">
        <property type="protein sequence ID" value="KAI7748096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAMZMK010006375">
        <property type="protein sequence ID" value="KAI7749391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G2N5"/>
    <dbReference type="SMR" id="A0AAD5G2N5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006846">
        <property type="entry name" value="Ribosomal_eS30"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12650">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S30/UBIQUITIN-LIKE PROTEIN FUBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12650:SF33">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN ES30Z_ES30Y_ES30X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04758">
        <property type="entry name" value="Ribosomal_S30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1 3" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="25"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU364011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7725869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7725877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7748096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7749391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="62" mass="6913" checksum="D19C19A092F5235F" modified="2024-05-29" version="1">MGKVHGSLARAGKVRGQTPKVAKQDKKKQPRGRAHKRIQYNRRFVTAVVGFGKKRGPNSSEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G4Q3</accession>
    <name>A0AAD5G4Q3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pectin acetylesterase</fullName>
            <ecNumber evidence="6">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_019030</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 6">Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 6">Secreted</location>
            <location evidence="2 6">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the pectinacetylesterase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="6"/>
    <dbReference type="EMBL" id="JAMZMK010011249">
        <property type="protein sequence ID" value="KAI7728247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G4Q3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009505">
        <property type="term" value="C:plant-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052793">
        <property type="term" value="F:pectin acetylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004963">
        <property type="entry name" value="PAE/NOTUM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21562">
        <property type="entry name" value="NOTUM-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21562:SF5">
        <property type="entry name" value="PECTIN ACETYLESTERASE 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03283">
        <property type="entry name" value="PAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0134">Cell wall</keyword>
    <keyword evidence="5 6" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7728247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="343" mass="37866" checksum="D76259E22B60ADBE" modified="2024-05-29" version="1">MNNTISECSDVGYNNPLYVGLTLIHGAAAKGAVCLDGTLPGYHLHRGYGSGANSWIVHLEGGGWCNSVRTCVYRKTTRRGSSNYFEKQLAFTGILSNKAQENPDFFNWNRVKIRYCDGASFTGDSEDKVHNLQFRGQKIWLAAIQELMSKGMRYANQALLSGCSAGGLASILHCDEFRGFFSRRTKVKCFADAGMFLDARDVAGGHTLRNMYNGVVTLQGAGRNLPRTCTNHLDPTSCFFPQNIISNLRTPMFLLNTAYDSWQIIASLAPPSADRRGAWKACQKNPANCSPSQMNFLQDNSPLLGNKAIALAVGDWFFDRAAVKKIDCPYPCDKTCHNLLFRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G4R1</accession>
    <name>A0AAD5G4R1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_033795</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011157">
        <property type="protein sequence ID" value="KAI7728740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G4R1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7728740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="148" mass="17772" checksum="A467B2960D78FDDF" modified="2024-05-29" version="1" fragment="single">MSSSEMGNHNHQFYIVKFLPGREEHEKEEADKQCHLMNRARFSITEEIDNLKAEEQHINRKITEFSLLDKELKWKMQEIEDLQHCERGIKHILPFMLEPVTTGFFRLPEIDAWTMIRQGQKRCQKRLIKKGRMDMEYLQQVKEQLIGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G5S2</accession>
    <name>A0AAD5G5S2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_003065</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010925">
        <property type="protein sequence ID" value="KAI7729759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G5S2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34121:SF9">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34121">
        <property type="entry name" value="MYOSIN-11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7729759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="81" mass="9338" checksum="9071441B3885C3A1" modified="2024-05-29" version="1" fragment="single">MNFLESTWDFQSLFVNQRDKQVNDQLQNHEAYIVNVVASFLSTYKDQLEPAIANLRKLLQSLKGYIVIGSSNSMYPLIVLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G805</accession>
    <name>A0AAD5G805_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">REF/SRPP-like protein At3g05500</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_010511</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the REF/SRPP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010362">
        <property type="protein sequence ID" value="KAI7731892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G805"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008802">
        <property type="entry name" value="REF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33732">
        <property type="entry name" value="REF/SRPP-LIKE PROTEIN OS05G0151300/LOC_OS05G05940"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33732:SF9">
        <property type="entry name" value="REF_SRPP-LIKE PROTEIN OS05G0151300_LOC_OS05G05940"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05755">
        <property type="entry name" value="REF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7731892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="241" mass="26655" checksum="3C58AB6483F1FE77" modified="2024-05-29" version="1">MAQNNVPTVTNQSQVEREEERLKYLQFVQMASLHALLYASKAYTYAKNNSGPLKPRVETVEGTLKTVVGPAYDKYHHVPVDVLKFVDRKVDESVNKFDSQVKPFVKQVSVKTKNLSAEVKTTGVVETASGLAKTAYTKIEPTAKGLYTKYEPVAEQYAASGWQSLNQLPLFPKVAKVVVPTAAYCSEKYNQTVQQTAEKGYKVSSYLPLVPTERIAKVFNPVEPQAETVVHTAGEGVVMAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G9W5</accession>
    <name>A0AAD5G9W5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_024227</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010212">
        <property type="protein sequence ID" value="KAI7732508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G9W5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="14"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7732508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="107" mass="11889" checksum="E60A5A8C00CBF6A2" modified="2024-05-29" version="1" fragment="single">SEELKPFENHHCDSLSTTTTTTVPLSPTTDEAATTNNNDPNSNPRQKTHLSQMIVRFWVSKGSRGVLGSKRNLGSGFRVKDMVYDGLVWEDDVWQGEDGEEDDSACD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GB67</accession>
    <name>A0AAD5GB67_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Stalled ribosome sensor GCN1-like HEAT repeats region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_026553</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009848">
        <property type="protein sequence ID" value="KAI7734036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GB67"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019887">
        <property type="term" value="F:protein kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034198">
        <property type="term" value="P:cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000357">
        <property type="entry name" value="HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057546">
        <property type="entry name" value="HEAT_GCN1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23346:SF7">
        <property type="entry name" value="STALLED RIBOSOME SENSOR GCN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23346">
        <property type="entry name" value="TRANSLATIONAL ACTIVATOR GCN1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02985">
        <property type="entry name" value="HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13513">
        <property type="entry name" value="HEAT_EZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23271">
        <property type="entry name" value="HEAT_GCN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25786">
        <property type="entry name" value="HEAT_GCN1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Stalled ribosome sensor GCN1-like HEAT repeats region" evidence="3">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="2">
        <location>
            <begin position="59"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7734036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="458" mass="49287" checksum="0A3B98CBE10C4D60" modified="2024-05-29" version="1" fragment="single">AAWEALSRVVGSVPKENLPSYIKLVRDAVSTCRDKERRKKKGGPIVIPGFCLPKALQPLLPIFLQGLISGSAELREQAAQGLGELIEVTSEKALKEFVIPITGPLIRIIGDRFPWQVKSAILSTLTIIIGKGGIALKPFLPQLQTTFVKCLQDSTRTVRSSAASALGKLSALSTRVDPLVGDLLSNLQASEGGVREAILAALKGVIKHAGKSVSAPVKTRVFDYLKELIYNDDDQIRGSSARILGILSEHLEDDQIFELLEELTDNASSASWFARHGALLTISSMLRHIPTMISASSSVTVVADCLKNNLKDEKFPVRETSTKALGRLLLHQVQADPSNANAHKGTIVSIASAMQDESSEVRRRALFALKAAAKENPTAIMVHVTVYGPVLAECLKDGSTPVRLAAERCALHALQLTKGTENVQAAQKYITGLDARRISKLSEFSDASDDSEDDSASG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GBD8</accession>
    <name>A0AAD5GBD8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Deoxyhypusine synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_030823</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the deoxyhypusine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009524">
        <property type="protein sequence ID" value="KAI7735249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GBD8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034038">
        <property type="term" value="F:deoxyhypusine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.910.10:FF:000002">
        <property type="entry name" value="Deoxyhypusine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.910.10">
        <property type="entry name" value="Deoxyhypusine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002773">
        <property type="entry name" value="Deoxyhypusine_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036982">
        <property type="entry name" value="Deoxyhypusine_synthase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00321">
        <property type="entry name" value="dhys"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11703">
        <property type="entry name" value="DEOXYHYPUSINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11703:SF0">
        <property type="entry name" value="DEOXYHYPUSINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01916">
        <property type="entry name" value="DS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7735249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="369" mass="40955" checksum="064AB595378E97BA" modified="2024-05-29" version="1">MEDAKVIENLRSVVLKESASLEGSCDRINGYDFNQGVNYSQLLKSFAFTGFQASNFGDAIQTVNQMLEWRLSHEQVTEDCSEEEKNPTYRESVKCKIFLGFTSNLISSGVRDVIRYLVQHHMVEVIVTTTGGIEEDIIKCLGDTYKGDFSLPGAALRSKGLNRIGNLLVPNDNYCKFEDWIIPIFDQMLVEQNTQHVLWTPSKLIARLGKEINNESSYLYWAYKNDIPVFCPGLTDGSLGDMLYFHSFRSPGLVVDVVQDIRAMNGEAVHANPRKTGMIILGGGLPKHHICNANMMRNGADYAVFINTAQEFDGSDSGARPDEAVSWGKIRASAKSVKVHCDATIAFPLLVAETFAATREKTTNPNSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GBM9</accession>
    <name>A0AAD5GBM9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_012100</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009258">
        <property type="protein sequence ID" value="KAI7736495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GBM9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00018">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.730.10:FF:000002">
        <property type="entry name" value="AP2-like ethylene-responsive transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.730.10">
        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001471">
        <property type="entry name" value="AP2/ERF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32467">
        <property type="entry name" value="AP2-LIKE ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32467:SF122">
        <property type="entry name" value="AP2-TYPE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00847">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00380">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51032">
        <property type="entry name" value="AP2_ERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="8">
        <location>
            <begin position="38"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="106"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="180"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="106"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="141"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7736495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="218" mass="24583" checksum="BEBC4E6ACB8782BF" modified="2024-05-29" version="1" fragment="single">MQLGTYKEELREMESLSKEEYIGSIRRKSSGFSRGVSKYRGVARHHHNGRWEARIGRVFGNKYLYLGTYATQEEAATAYDMAAIEYRGLNAVTNFDLSRYIKWLRPNQTDPNNSNGIPTNSDPNLTQDPEPTFSYNNHQPETSSSGETTTSVLPPLRTTATSSAVGLLFKSSKFKEMLELTSAVDSPSPSPESDLPRRNFPDDIQTYFDCHESNSYAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GC70</accession>
    <name>A0AAD5GC70_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">(+)-abscisic acid 8'-hydroxylase</fullName>
            <ecNumber evidence="14">1.14.14.137</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">M8C21_010464</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">AA19_3_7</strain>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>2-cis-(+)-abscisate + reduced [NADPH--hemoprotein reductase] + O2 = (+)-8'-hydroxyabscisate + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12897"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:37569"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:58490"/>
            <dbReference type="EC" id="1.14.14.137"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 15">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="13">Plant hormone degradation; abscisic acid degradation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.14.137" evidence="14"/>
    <dbReference type="EMBL" id="JAMZMK010009645">
        <property type="protein sequence ID" value="KAI7734773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GC70"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010295">
        <property type="term" value="F:(+)-abscisic acid 8'-hydroxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046345">
        <property type="term" value="P:abscisic acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009737">
        <property type="term" value="P:response to abscisic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009414">
        <property type="term" value="P:response to water deprivation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016125">
        <property type="term" value="P:sterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11043">
        <property type="entry name" value="CYP90-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000014">
        <property type="entry name" value="Abscisic acid 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24286:SF375">
        <property type="entry name" value="ABSCISIC ACID 8'-HYDROXYLASE 4-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24286">
        <property type="entry name" value="CYTOCHROME P450 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 15 16" id="KW-0349">Heme</keyword>
    <keyword evidence="9 15 16" id="KW-0408">Iron</keyword>
    <keyword evidence="11 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 15 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 16" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="8 16" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="15">
        <location>
            <position position="417"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00050609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00060633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00066338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAI7734773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="476" mass="55089" checksum="41EAE47CB8C3CAFF" modified="2024-05-29" version="1">MKLLATIFYSIIFLSSLFSYYFFRKEKRLHKTRAKLPPGSMGWPCIGETLQMYTQDPNVFFASKQKRYGDIFKTQILGYPCVMLASPEAARFVLVSQAHLFKPTYPKSKEKLIGPSALFFHTGDYHARMRKLVQSSFSPEVTRKLIPEIEKIALSSLESWANGQVINTFHEMKKFAFEVGVLSIFGQLNKTYTNKLKENYSILEKGYNCFPTKLPGTAYHRSLMARRRLNQILSEIMNERKEKKLVTNNLLRHMLNFKDEKGNTLSDEQIADNIIGVLFAAQDTTASVLTWILKYLSDDPKLLDMVKGEQKAICESEYGENRSLTWSMTRKMPLTYRVILESLRKASVISFTFREAVVDIEYEGYLIPKGWKVMPLFRNIHHNPEFFAEPENFDPFRFENPPKPNTYIPFGNGIHACPGNELAKLEMLVLLHHMLTKFRWELVGPSDLVQYSPFPVPEQGLRARFIKEPTANKCYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GCT7</accession>
    <name>A0AAD5GCT7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_017821</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009476">
        <property type="protein sequence ID" value="KAI7735463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GCT7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05121">
        <property type="entry name" value="ABC1_ADCK3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004147">
        <property type="entry name" value="ABC1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051130">
        <property type="entry name" value="Mito_struct-func_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43173">
        <property type="entry name" value="ABC1 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43173:SF12">
        <property type="entry name" value="PROTEIN KINASE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03109">
        <property type="entry name" value="ABC1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="132"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7735463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="443" mass="49943" checksum="081206DE684F04AD" modified="2024-05-29" version="1" fragment="single">MPLDFKDIQEKISVGFKPWHRSFQFWVRALDIYTDYKVFQVRVNFEKDEKKAEAMWERQHEHAADKVYSMCSDMGGFFLKLAQILGKPDLAPAAWVRRLVTLCDQAPATPSNMVQLVIEEELCKNVDEMFERFEWSPIGSASIAQVHRARLKGDKNDVVVKVQHPGVQDLMMTDLRNMKAFALFIQKTDVKFDFFSKWRNRRVLVMEYIDGVPILNLGDEIKKRGINPDGKMAAAAKQNILKSLTLAYGQMILKSGFFHADPHPGNILICNGSGVALLDYGQVKDLPNALRLGYANLVLAIADNDHARASEGFRELGIDTLSLCSNEQAELFKLAQVMFDTKLPPGVKVLQPFSDDSSIKKVAVQAFPEELFSVLRTVQLLRGLSVGLGINYSCAEQWRPIAEEALCHAGRLKVEDMKTREGSKHGIVVSTRHTTCHSCNKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GDN5</accession>
    <name>A0AAD5GDN5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_030391</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008809">
        <property type="protein sequence ID" value="KAI7738477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GDN5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37610">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37610:SF103">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 6 REGULATORY ANKYRIN REPEAT SUBUNIT C-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7738477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="265" mass="30195" checksum="F9A0FD7883021F4F" modified="2024-05-29" version="1" fragment="single">MEIGGSTNAPDVSATTQDLKFMMESSVNVSNFVSVKLSGHRNYKIWKAQLLCLIKSQALLHFIDAEHPFPGDNGMTAQYDELVKGWIFGSVKEKVLKDLVDLGTAQEVWMKLGSLFNQPTSNTEGTSSNALDVSAPDAIAPVVYAMREDLKYLQALNVDVSNFVSEKLVGKINYHTWRTEMYHLIESHELLHIIHAEEARFPWDEGDPMTQKYDSLVRGWILNTMNINRHDEYRIYRSVQLMWRKLESTFTYQPEISNGNTGDTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GE03</accession>
    <name>A0AAD5GE03_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 18">Peroxidase</fullName>
            <ecNumber evidence="4 18">1.11.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">M8C21_018600</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">AA19_3_7</strain>
            <tissue evidence="21">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 18">
            <text>2 a phenolic donor + H2O2 = 2 a phenolic radical donor + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:56136"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:139520"/>
            <dbReference type="ChEBI" id="CHEBI:139521"/>
            <dbReference type="EC" id="1.11.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15 18">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="15 18">Binds 2 calcium ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15 18">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
        <text evidence="15 18">Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="18">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peroxidase family. Ascorbate peroxidase subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.7" evidence="4 18"/>
    <dbReference type="EMBL" id="JAMZMK010008713">
        <property type="protein sequence ID" value="KAI7738852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GE03"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140825">
        <property type="term" value="F:lactoperoxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00693">
        <property type="entry name" value="secretory_peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.420.10:FF:000006">
        <property type="entry name" value="Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.420.10">
        <property type="entry name" value="Peroxidase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002016">
        <property type="entry name" value="Haem_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000823">
        <property type="entry name" value="Peroxidase_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019794">
        <property type="entry name" value="Peroxidases_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019793">
        <property type="entry name" value="Peroxidases_heam-ligand_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033905">
        <property type="entry name" value="Secretory_peroxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31388:SF204">
        <property type="entry name" value="PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31388">
        <property type="entry name" value="PEROXIDASE 72-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00141">
        <property type="entry name" value="peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00458">
        <property type="entry name" value="PEROXIDASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00461">
        <property type="entry name" value="PLPEROXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00435">
        <property type="entry name" value="PEROXIDASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00436">
        <property type="entry name" value="PEROXIDASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50873">
        <property type="entry name" value="PEROXIDASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 15 18" id="KW-0106">Calcium</keyword>
    <keyword evidence="11 17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 18" id="KW-0349">Heme</keyword>
    <keyword evidence="18" id="KW-0376">Hydrogen peroxide</keyword>
    <keyword evidence="10 15 18" id="KW-0408">Iron</keyword>
    <keyword evidence="19" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 15 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 18" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5 18" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="18" id="KW-0964">Secreted</keyword>
    <keyword evidence="18" id="KW-0732">Signal</keyword>
    <keyword evidence="19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041774737" description="Peroxidase" evidence="18">
        <location>
            <begin position="29"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="89"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="Plant heme peroxidase family profile" evidence="20">
        <location>
            <begin position="29"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="13">
        <location>
            <position position="70"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="15">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="16">
        <location>
            <position position="66"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="39"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="191"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU362060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAI7738852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="308" mass="34030" checksum="0CEE7F52321D96FB" modified="2024-05-29" version="1">MALSTFSSFFKLSTFTLCLLVMFAPSYGQLTANFYARTCPNFRSIITRAVNSAVANEARMGASLLRLHFHDCFVNASILLLNINLPFQLTFLCFYFEPCHVIILVSFYIILPFSQSHYNYYFSHLQLRGPGWNVLFGRRDSTTASLSAANSNLPSPASSLSTLISSFSNQGFTANEMVALSGSHTIGQARCTTFRNRLYNDNNINASFATALRPNCPSSGGDNNLSPLDSTATSFDNRYFNDLINQRGLLHSDQELFNGGSTDAQVRTYSSNPATFNRDFASAMVKMSNLSPLTGSSGQVRTNCRRTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GFN5</accession>
    <name>A0AAD5GFN5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_012260</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="5">Interacts with RBR1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008828">
        <property type="protein sequence ID" value="KAI7738328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GFN5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010052">
        <property type="term" value="P:guard cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010235">
        <property type="term" value="P:guard mother cell cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010444">
        <property type="term" value="P:guard mother cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009554">
        <property type="term" value="P:megasporogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050891">
        <property type="term" value="P:multicellular organismal-level water homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901333">
        <property type="term" value="P:positive regulation of lateral root development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901002">
        <property type="term" value="P:positive regulation of response to salt stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902584">
        <property type="term" value="P:positive regulation of response to water deprivation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902806">
        <property type="term" value="P:regulation of cell cycle G1/S phase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032875">
        <property type="term" value="P:regulation of DNA endoreduplication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000037">
        <property type="term" value="P:regulation of stomatal complex patterning"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009629">
        <property type="term" value="P:response to gravity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009725">
        <property type="term" value="P:response to hormone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033993">
        <property type="term" value="P:response to lipid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048364">
        <property type="term" value="P:root development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010376">
        <property type="term" value="P:stomatal complex formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000355">
        <property type="entry name" value="Transcription factor MYB124"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050560">
        <property type="entry name" value="MYB_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614">
        <property type="entry name" value="MYB PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614:SF76">
        <property type="entry name" value="TRANSCRIPTION FACTOR MYB124"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13921">
        <property type="entry name" value="Myb_DNA-bind_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="HTH myb-type" evidence="7">
        <location>
            <begin position="7"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="6">
        <location>
            <begin position="14"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="7">
        <location>
            <begin position="59"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="6">
        <location>
            <begin position="59"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00063045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7738328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="389" mass="43877" checksum="3820F27B72FFE113" modified="2024-05-29" version="1" fragment="single">NGEGQKQKERHIVSWSQEEDDILREQIRMHGTDNWAIVASKFKDKSTRQCRRRWFTYLNSDFKKGGWSQEEDMLLCEAQKIFGNRWTEIAKVVSGRTDNAVKNRFSTLCKKRAKNEALAKENNKFYTATSNKRNRLNNVRDSETVLETSMSPSKIRRKHIPDTTEGYQQPRPPFAVILQKCHNINASTTQLHPKSEEVSKDGTFLKKDDPKIIALLQQAELLSSLAVKVNTEKTNESYEDACKVVQDFLKQSKGSDALGLSASDIDIRSCDEGSQQSWRQLDLYQGSPDGSEYSTGSTVLPQAVEKMEPAQDNIVDGKSNIMPDSTINEDILGLCDGFDNDVDVVCPLPNSEFNSPIQVTPMFKSMAAGIPSPQFSESVSFVYGLRVWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GG34</accession>
    <name>A0AAD5GG34_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycosylphosphatidylinositol anchor attachment 1 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_021147</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008265">
        <property type="protein sequence ID" value="KAI7741132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GG34"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042765">
        <property type="term" value="C:GPI-anchor transamidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016255">
        <property type="term" value="P:attachment of GPI anchor to protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007246">
        <property type="entry name" value="Gaa1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13304">
        <property type="entry name" value="GLYCOSYLPHOSPHATIDYLINOSITOL ANCHOR ATTACHMENT 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13304:SF0">
        <property type="entry name" value="GLYCOSYLPHOSPHATIDYLINOSITOL ANCHOR ATTACHMENT 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04114">
        <property type="entry name" value="Gaa1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036762">
        <property type="entry name" value="GAA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="462"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="505"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="541"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="573"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="618"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="660"/>
            <end position="685"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7741132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="688" mass="76139" checksum="832523506CB5FC3D" modified="2024-05-29" version="1">MTSTPETEAQKPKHRPIIRIGIFIIAHSFIVSVVCCTAGVLALLLLPVLAKKTYVSENALMPGSAIPMISEQDVSGANKFINELTSLKLKSQRTGIEIPRLIALHMANLGGEVSYHKFQNQPNKFHPLHFFTSTELEANHVNQTCESYATNTVGIIRAPRGDGKEAIVLVTPYNPVNFSQNEALSLGIAYSVFSLLNQVTWLAKDIIWLAADSRYGQYNSVAAWLKDYHTPSFGGFGKLHGVGCHNGNDVPESNLDMIIETEVSDTLRRAGTMAAALVIKVAKTGEAGDQDALNIYAEASNGQMPNLDLINVVSYLAVHGQGLRVQVDKLVSFLDSKWLKFIGGVLETVGNIARTLNPQWKFGIPVKEYVEGSATLVSSLYNQALGVPTGSHGAFRDYQVDAITIEISPKLFSTSKGRQTELLFRGGRLVEGVIRSVNNLLEKFHQSFFLYLLTSSNKFVSVGVYMIAFLLLVAPLPVLAASLFSDANKSNLESEKNKPFKSWRWLYPTKTVFLVHLWGAIVSLLPYFIYQIPDSQPTSRLLIWASLSLFILIVLGSFFRVRSTQWALLKSVTVSAAFIGLCLMSVINFATAEIGALLLVPMCLMIKPWRVAGKFQSALNLVLVIIGFPGATFFVVKSAFGFQNESSLVEFWYWVESLWAWNSATYIYICMVHLPCWVLCIYILLHQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GI76</accession>
    <name>A0AAD5GI76_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Mechanosensitive ion channel MscS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_018479</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MscS (TC 1.A.23) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008255">
        <property type="protein sequence ID" value="KAI7741161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GI76"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008381">
        <property type="term" value="F:mechanosensitive monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050982">
        <property type="term" value="P:detection of mechanical stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006820">
        <property type="term" value="P:monoatomic anion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.60:FF:000003">
        <property type="entry name" value="Predicted mechanosensitive ion channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016688">
        <property type="entry name" value="MscS-like_plants/fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023408">
        <property type="entry name" value="MscS_beta-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006685">
        <property type="entry name" value="MscS_channel_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31618:SF1">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31618">
        <property type="entry name" value="MECHANOSENSITIVE ION CHANNEL PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00924">
        <property type="entry name" value="MS_channel_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017209">
        <property type="entry name" value="Memb_At2g17000_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0407">Ion channel</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="186"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="225"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="258"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="300"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="570"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="602"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="domain" description="Mechanosensitive ion channel MscS" evidence="11">
        <location>
            <begin position="621"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="91"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="136"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="20"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="39"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="103"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="139"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="782"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7741161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="782" mass="89111" checksum="BD2D0199A6B6FB88" modified="2024-05-29" version="1" fragment="single">NQLQTQTPPIPLNDVVNSFPIPPPPPSPPCVRHRLRRTPATTQTTSFDFKTSDTPSPQQSPALSRIPESPNNYGNLTPKEVRVSFHEEVVEPAPVRQRSNGSGRTGAGTGGSGEEVLVCSGNGSFKAKSSLLRTKTKSRLMDPPEADQRSGRTPKGGGSEFDEDDPFLDDDLPDEYKELRYSKWTLLQLFSLVLIVGGLVCSLTIDYLKHKYLFDLELWKWGVMVLVLICGRLVSGWGIRVIVVLIERSFVLRKKVLYFVYGLRKAVQNCVWLTLVLIAWQCIFDKKVQRVTKGSDVLPYVTRIWVCLLVATIFWLLKTLLVKVLASSFHVSTFFDRIQESLFNQYVIESLSGPPLIEIQHEREEEDRMMEEVQKLQKAGATLPADLKANIFKKSGRFIGTSRTNTPKVGKSGKFSEAATPRKVDEGITIDHLHRLNQKNISAWNMKRLMNIVRTGVLSTLDEQLEGTTRGEDENAGEITNEKQAKAAAKKIFRNVAKPGSKRIYLEDLMRFLREDEALKAIRLFDAESETKGISKGALKNWVVNVFRERRALALSLDDTKTAVNKLHQMMNVAVGILIIMIWLLILKVATTKNFIFLSSQLLLVVFVFGNTCKTTFEAIIFLFVMHPFDVGDRCEIDSVQMVVEEMNILTTVFLRYDNQKIIYPNSVLATKPIANYYRSPDMGDAIDYCIHVSTPVEKVALMKERITSYIENKSDHWYPAPMIIVRDVEDMNRLKISIWLNHRMNFQDMGERWQRRALLVEEMIKIFRELDIEYRMLPLDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GIH4</accession>
    <name>A0AAD5GIH4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PGG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_022472</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008281">
        <property type="protein sequence ID" value="KAI7741043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GIH4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026961">
        <property type="entry name" value="PGG_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24177">
        <property type="entry name" value="CASKIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24177:SF472">
        <property type="entry name" value="PGG DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13962">
        <property type="entry name" value="PGG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="603"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="641"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="domain" description="PGG" evidence="3">
        <location>
            <begin position="593"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="273"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7741043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="667" mass="74997" checksum="5CA51ECFCF782DA7" modified="2024-05-29" version="1" fragment="single">MMNQAGAQLPIIDEHKPLSQRQQQQVNIPSVSHANPHPPPKLPRSDLINGSREDYLKIGVPLYEASIKCDWKAAKAIFDTNLELVRCSITENGETSLHVAASAKDPKDVEKFVKNLMDMMAKEDLELQNKNHNTAMYLAAVAGNIKAVKIMVEKNESLLIIAGGNGIMMPLYAAALFGNEDVVKYLYNKSKRLGDDGWTSQNRGWLLDKCVENDMFDVALEIVKTYPELCTGSVLRLLARKPEAFPETKFNIISRTISWGKYLFSKMLISQHPSQSEDAGSGSGPPESSQTHEPFPEIESNNIIRIIKSVFIRFKWGSHEKEAQALQLLRIIWGNIAKKSKKEIDDIIRGPKDKNQDDKPASGTMDKTLELQKLVSEHIANMHVEIQNNIIKRLPTTEMDDQTLQLQNLISERIAKMHVETQSLLKGPTKTLNNPNVASSGKTTKTTKTYSSRILFIAAEMGNTRFVVELIRQYPDLIWKVNDNNQSIFHIAVKHRHEGIYNLLYEIGSMKDLITPLKDEKENNMLHLVGKSAKKKRLEDVSGVALQMQRELLWFKEVESMIPPSYGERKNKDGLTPYELFTKEHKDLVTQGEKWMKDTASQCMVVAALIATMVFAAAFTVPGGYDQNDGIPIFYPKATFMVFVMADAISLFASSASILMFLSILTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GIJ0</accession>
    <name>A0AAD5GIJ0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Non-haem dioxygenase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_011800</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008170">
        <property type="protein sequence ID" value="KAI7741566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GIJ0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050295">
        <property type="entry name" value="Plant_2OG-oxidoreductases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47991">
        <property type="entry name" value="OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Non-haem dioxygenase N-terminal" evidence="3">
        <location>
            <begin position="38"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7741566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="149" mass="17646" checksum="3897A46BE2A062D5" modified="2024-05-29" version="1">MANLVSTWSKTTQILPENYIFPVDQRPGSVEIPSCQNIPLIDLSASQCQTVQQIMYACQEFGFFQVINHGVSEYLIDEAMEVVKEFFNMAEEDKEKYYSIDPIKKCKVYTSGYDYVNEEIHFWRDNLRHPCHPLDEWVHLWPERPTNYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GIT4</accession>
    <name>A0AAD5GIT4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_022027</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007917">
        <property type="protein sequence ID" value="KAI7742749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GIT4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048440">
        <property type="term" value="P:carpel development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00265">
        <property type="entry name" value="MADS_MEF2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1810.10:FF:000004">
        <property type="entry name" value="MADS-box transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1810.10">
        <property type="entry name" value="Transcription factor, MADS-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050142">
        <property type="entry name" value="MADS-box/MEF2_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033896">
        <property type="entry name" value="MEF2-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002487">
        <property type="entry name" value="TF_Kbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002100">
        <property type="entry name" value="TF_MADSbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036879">
        <property type="entry name" value="TF_MADSbox_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48019">
        <property type="entry name" value="SERUM RESPONSE FACTOR HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01486">
        <property type="entry name" value="K-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00319">
        <property type="entry name" value="SRF-TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00404">
        <property type="entry name" value="MADSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00432">
        <property type="entry name" value="MADS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55455">
        <property type="entry name" value="SRF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51297">
        <property type="entry name" value="K_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00350">
        <property type="entry name" value="MADS_BOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50066">
        <property type="entry name" value="MADS_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MADS-box" evidence="7">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="K-box" evidence="8">
        <location>
            <begin position="88"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="183"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="199"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7742749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="251" mass="28872" checksum="1F22410EB9C52925" modified="2024-05-29" version="1">MGRGRVELKRIENKINRQVTFAKRRNGLLKKAYELSVLCDAEVALIIFSNRGKLYEFCSTSNMLKMLERYQNCTYSSLEVNRSANDAEQSSYKEYIKLKAKYESLQQYQRQIFGEDLSPLNLKELEQLERQLDSTLKQIRSIRTQSMLDQLSDLQAKERMWLEANKGLQYKLEEYYAENQAGPSWAGGENGNSFGQHHQNQEHHPQHHEGFFQPLDCTNLQIGYNAIGSSSQMTASTSGQNVSGIIPGWML</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GJX8</accession>
    <name>A0AAD5GJX8_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_003368</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007991">
        <property type="protein sequence ID" value="KAI7742438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GJX8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="201"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="208"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7742438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="244" mass="27195" checksum="5D9641160E1EF554" modified="2024-05-29" version="1">MNGIWGEVLLDYHVEGCWLKAVGGEGVEQAKAGGGVGRQELMTAKRCSLQHMMELFRTDQISWHRLPQNSLESMQMKELTDPLNSVILTDGSDKWKWEGDKDGEFSVADCKKQTLKGLYLRYRIPITIDDKTDFLDATVFDKAGIQLLEIPCKEMFAKPDPRTLIREKITAKPIQFTIRAVKDDQTALTTYSVNNVVRLQPNKNTDTSSSSSVQISATPAPLTPAPANPSINEKKGCYCKAQTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GK75</accession>
    <name>A0AAD5GK75_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pectin acetylesterase</fullName>
            <ecNumber evidence="6">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_005545</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 6">Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 6">Secreted</location>
            <location evidence="2 6">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the pectinacetylesterase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="6"/>
    <dbReference type="EMBL" id="JAMZMK010007092">
        <property type="protein sequence ID" value="KAI7745917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GK75"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007142">
        <property type="term" value="P:male meiosis II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039300">
        <property type="entry name" value="JASON"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004963">
        <property type="entry name" value="PAE/NOTUM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33318">
        <property type="entry name" value="ASPARTYL/GLUTAMYL-TRNA(ASN/GLN) AMIDOTRANSFERASE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33318:SF7">
        <property type="entry name" value="PROTEIN JASON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03283">
        <property type="entry name" value="PAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0134">Cell wall</keyword>
    <keyword evidence="5 6" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="7"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="44"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7745917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="425" mass="47304" checksum="2DDD05EEF498F61D" modified="2024-05-29" version="1">MWGYAEGSERVKAGEEKSEPLPATKIRKEGRGNKMGEFVGEEGGGNDDVDMEDGLFKTPGNRANVDQPEVTPVNVSDGQAVQKETVDEFNAFDGVSKVDLELSNSEMEGSGNVIDEVSKSELEFANSNLEGGSNAGENCKEYVTVGPTVGVKRKKVKNHIVLEQLNSGGPHSSNDRPMKEPKKGVLIDEAEDVECEPSTVHVEDLVSKGEDLDTDEHSDKAIELEVANTKNLGVKMGVQLEDEVTDRSYTTRTPVDMPILGMVAPHWNADETVDPPAPPKWWDGNGFPNSTNKYKEDQKVSWHATPFEERLEKALSEDRLIAERYAWMELYLGIIYIADMVPGANSWIVHLEGRGWCNSVRTCVYRKTTRRGSSNYFEKQLAFTGIMNNKAQENPDFFNWNRVKIHYCDGVSFTGDSEDKVDTRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GL05</accession>
    <name>A0AAD5GL05_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Benzyl alcohol O-benzoyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_023750</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the plant acyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007427">
        <property type="protein sequence ID" value="KAI7744849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GL05"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050898">
        <property type="entry name" value="Plant_acyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31147">
        <property type="entry name" value="ACYL TRANSFERASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31147:SF66">
        <property type="entry name" value="OS05G0315700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02458">
        <property type="entry name" value="Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7744849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="442" mass="49103" checksum="3D8BAD1A1F6C340C" modified="2024-05-29" version="1">MTNKYCTALTFNVRRHTPELIAPATSTPREVKPLSDIDDQAGLQHLIPTIHFHRRNPNMGNKDPASVIRDALAKVLVFYYPLAGRIKEGHGKKLMVDCTGEGVLFIEAEADVTLEEFGKTLHPPFPCTEKLLCDVPGSGGIIGSPLVQIQVTRLLCGGFIFTARIYHTMCDGTGAVQFLMALGEMAQGALEPSVLPVWQRELLNARDPPRVTFPHPEYDDVVDMEDNVMAIGEMADTSFFFGPAEMSALRRLVPKNLKTCTTFEVLTACLWRCRTIALQPDPEHDVRLMFFVNVRGMFDPPIPVGYYGNCIVIPCVVSKAKDLCNKPFSHALELVMEGKSRVTKEYVRSTMDLMVVKGRPSFKTSSTYIVSNLTRVGFNKVNFGWGEPLYAGLPNDDEDLPGVYSSYMPSTNDNGEFGIVARIALPISTMEKFVNELNIMLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GL52</accession>
    <name>A0AAD5GL52_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_008250</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007560">
        <property type="protein sequence ID" value="KAI7744251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GL52"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7744251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="38" mass="4578" checksum="2211BD45CD20D0CF" modified="2024-05-29" version="1">MRTTLSYFFLELLFKCSFGIINRKSRTGLYKRASQSWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GLA4</accession>
    <name>A0AAD5GLA4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_033324</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006825">
        <property type="protein sequence ID" value="KAI7747145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GLA4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7747145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="120" mass="13310" checksum="5B98DB6A2C7D6ED7" modified="2024-05-29" version="1">MTPVDQWGIQLSIITPVICVVYHLPHPYLAHSSNLHLPTAALIYIQIPIFVSVSVMLSVTMSVVAGFLCETLGGGGYRRRGFLLRGFFKSKTGLWCCWTTMMGARVRWEKAGKLSSPLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GLE4</accession>
    <name>A0AAD5GLE4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ribosomal protein L18e/L15P domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_012840</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007497">
        <property type="protein sequence ID" value="KAI7744566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GLE4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.100.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7744566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="31" mass="3431" checksum="F244D76A9AC8134F" modified="2024-05-29" version="1" fragment="single">MGIDLVAGGKSKRTKRTAPRSDDIYLKLLVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GLY4</accession>
    <name>A0AAD5GLY4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Multiple inositol polyphosphate phosphatase 1</fullName>
            <ecNumber evidence="4">3.1.3.62</ecNumber>
            <ecNumber evidence="3">3.1.3.80</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">2,3-bisphosphoglycerate 3-phosphatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">M8C21_010609</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">AA19_3_7</strain>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>(2R)-2,3-bisphosphoglycerate + H2O = (2R)-2-phosphoglycerate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:27381"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58248"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="EC" id="3.1.3.80"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:27382"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>1D-myo-inositol 1,2,4,5,6-pentakisphosphate + H2O = 1D-myo-inositol 1,2,5,6-tetrakisphosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:77115"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57798"/>
            <dbReference type="ChEBI" id="CHEBI:195535"/>
            <dbReference type="EC" id="3.1.3.62"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:77116"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>1D-myo-inositol 1,2,5,6-tetrakisphosphate + H2O = 1D-myo-inositol 1,2,6-trisphosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:77119"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:195535"/>
            <dbReference type="ChEBI" id="CHEBI:195537"/>
            <dbReference type="EC" id="3.1.3.62"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:77120"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>1D-myo-inositol hexakisphosphate + H2O = 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:16989"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57798"/>
            <dbReference type="ChEBI" id="CHEBI:58130"/>
            <dbReference type="EC" id="3.1.3.62"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:16990"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the histidine acid phosphatase family. MINPP1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.62" evidence="4"/>
    <dbReference type="EC" id="3.1.3.80" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010007141">
        <property type="protein sequence ID" value="KAI7745779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GLY4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003993">
        <property type="term" value="F:acid phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034417">
        <property type="term" value="F:bisphosphoglycerate 3-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052745">
        <property type="term" value="F:inositol phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07061">
        <property type="entry name" value="HP_HAP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1240:FF:000017">
        <property type="entry name" value="Histidine acid phosphatase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000560">
        <property type="entry name" value="His_Pase_clade-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20963:SF8">
        <property type="entry name" value="MULTIPLE INOSITOL POLYPHOSPHATE PHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20963">
        <property type="entry name" value="MULTIPLE INOSITOL POLYPHOSPHATE PHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00328">
        <property type="entry name" value="His_Phos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="197"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="289"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="210"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="15">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00043668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00043671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00043691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00043832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAI7745779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="927" mass="103698" checksum="EF38F1411884AE31" modified="2024-05-29" version="1" fragment="single">SFSFREALTGERDYNRSNAKVLQFGDRDSKLLELSGRSLVVTVASFNLLKSFKVELEEMGLKEGVIRYLGGLKFLVSFLNAEHAIMAKDELLGRRDFFDHVEVWEGQLLGFERIAWVQICGMPVQLFSDENFEEVASLFGKVVHKCQRDDSGYDVSEFFAGIIVDKGDSIKGEVSILWKDKMFSVWVKEIAEDKVPFPVDNDSEVNKEDKDDDESSESDEDSMSEDQRSEDQDVGDSETVQPVNAPASVNDEINAPSEDCDNQGGNNVEEFTTPIISEVIIDQVKGNNKRKKNKGLKPLGLESGSYTSSNDRPTKESRLEEDDPFGLAPIILGRNITFRRSGPDPTEASLDPLFGDLEKQTTNSSAPVDLDNAVACEIVCPSPGNKLEEEIAGTSKLGFKKDPYSSWRRILEAIHGGKGKWDFLPVNRRLTGCWKSIVSCVSKVRLGGKDTSGGKRLAENAPNLQYEVSKDIKENAFDSSKIPDQCSPIHLNLVARHGTRAPTKKRMRDLEILSVQLKSLLQNAKEKGASLEKLPSWLWGWSSPWNGKLKGGELIIHGEDEMYHLGIRIRERFQELFSDDYHPDVYQIRATQIPRASASAVAFGMGMFSGRGKLGAGNHRAFAVLSESRASDIVLRFHDCCQNYKVYRKQQEPAVYKLKEPVLDEITRSLAARYELNFTIKDTSTLWFLCKQEASLLDITDQACGLFSNDELSLLEWTDDLEMFKLKGYGNSLNYRMGVPLLEDVILSMDTAIKAKEEGHAPGSYEKARLRFAHAETLVPFSCLIGLFLDGSEFDQIQREEALESPPKPPQKRTWRGSTVAPFAGNNALVLYSCLENDTSKYFVQVLHNEQPIPMAGCGGTDFCPFQVFKEKIAAAHLKHSYDNLCNVCTEKGPGTKSYTSQLFEMLSWILSGKNKDKVKASPKLEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GMJ9</accession>
    <name>A0AAD5GMJ9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phosphoglycerate mutase-like protein AT74H</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_018134</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007333">
        <property type="protein sequence ID" value="KAI7745168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GMJ9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001345">
        <property type="entry name" value="PG/BPGM_mutase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052765">
        <property type="entry name" value="PGM-Related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46192">
        <property type="entry name" value="BROAD-RANGE ACID PHOSPHATASE DET1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00175">
        <property type="entry name" value="PG_MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="1">
        <location>
            <position position="74"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="1">
        <location>
            <position position="162"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="73"/>
            <end position="80"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7745168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="290" mass="33535" checksum="E3BDA858AA0FE999" modified="2024-05-29" version="1">MNIPQLTPQTPTSPCTAMSITASSSIKCCEDTVGRRKRLSIDDTGLLTFPEKNLILHVPTAKPPRPRRIILVRHGQSEGNVDEGVYTRVADPKIRLTERGKREAEECGVRIREMIEKDGADDWKVYFYVSPYERTIETLRGLGKAFQRSRIAGVREEPRLREQDFGNFQDREQMKIQKAVRVQYGRFFYRFPNGESAADVYDRITVEQFERLNNMRNGNEIVMQTGQGGRYSLLVHHTQQQMIDFGLTQQMLDDQIWQKTAKPGELNYDYLTNGPSFFTHFDDENEAIFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GN41</accession>
    <name>A0AAD5GN41_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">O-fucosyltransferase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_032791</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase GT106 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006451">
        <property type="protein sequence ID" value="KAI7748870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GN41"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006004">
        <property type="term" value="P:fucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019378">
        <property type="entry name" value="GDP-Fuc_O-FucTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052272">
        <property type="entry name" value="GT106_glycosyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31933">
        <property type="entry name" value="O-FUCOSYLTRANSFERASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31933:SF5">
        <property type="entry name" value="O-FUCOSYLTRANSFERASE 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10250">
        <property type="entry name" value="O-FucT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-0294">Fucose metabolism</keyword>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00030350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7748870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="155" mass="17191" checksum="61C074EAB52B52C5" modified="2024-05-29" version="1">MNSRFTDEELRNQGRCSLTPEEIGLLLAALGFDNTTRLYLASHKVYGGEARIAALRQLFPYMEGKKSLASSAERAKIKGKASLSAAVDYYVSMHSDIFISAKYAQCYGRTPDVPQLEDNKTKHGVIGSAFHEQKHELVRFSRSGCGRATEQAWGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GNQ5</accession>
    <name>A0AAD5GNQ5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PRONE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_006272</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006344">
        <property type="protein sequence ID" value="KAI7749530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GNQ5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.2010:FF:000004">
        <property type="entry name" value="Rop guanine nucleotide exchange factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.2010:FF:000001">
        <property type="entry name" value="Rop guanine nucleotide exchange factor 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2010">
        <property type="entry name" value="PRONE domain, subdomain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005512">
        <property type="entry name" value="PRONE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038937">
        <property type="entry name" value="RopGEF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33101">
        <property type="entry name" value="ROP GUANINE NUCLEOTIDE EXCHANGE FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33101:SF1">
        <property type="entry name" value="ROP GUANINE NUCLEOTIDE EXCHANGE FACTOR 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03759">
        <property type="entry name" value="PRONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51334">
        <property type="entry name" value="PRONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRONE" evidence="4">
        <location>
            <begin position="62"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="431"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="519"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="16"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="447"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="484"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="525"/>
            <end position="537"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7749530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="563" mass="62697" checksum="0A8A740A853E288A" modified="2024-05-29" version="1">MENNSGDGFEFESFDSRTNSTSSKESCSSPASLGWPIRKAQMMTNSQEKLQSDTCLNDELKSKKTGAQVSEMEMMKERFSKLLLGEDMSGSGKGVCTALAISNAITNLYATAFGQLWRLEPLPSEKKHMWRREMECLLCVSDHIVEFKPSWQTLADGSKLEIMTCRPRSDIFVNLPALRKLDNMLLGILDSFTNTEFWYVDKGITTPEPDSPASFHKPLQRQQDKWWLPVPRVPAGGLDETARKRLNRKRESANQILKAAMAINSVSLSEMEVPESYLESLPKNGRACLGDVIYQYITSEQFSAECLLDCLDLSSEHVALEIANRVEAAIYMWRRKPPPAKTSWDMVKDLMADAHKRDLLADRAESLLLCLKHRFPCLTQTSLDTTKIQCNKDVGKAILESYSRVLESLAYNIVARIDDLLYVDDLSKQSESRPSRVSTSSHKRVSVSMSTTSGSPFRSGFGTPKFSPAPLVTPSPARGDRTPFLTNNGNSNKTTSRGCGVRRALTSYLGGETRVKSIEGPGCLSTRNSDTPSSRTSVDGALSQKENRTPVKLQKRIAELQVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GQF7</accession>
    <name>A0AAD5GQF7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_016017</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005943">
        <property type="protein sequence ID" value="KAI7751255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GQF7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7751255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="130" mass="15638" checksum="A9A23288DA85CFFD" modified="2024-05-29" version="1">MRTRVIALNLQQEFRNLINQLKHDYENRIKELLVQINSTKISCAHRNHVEALAKRLDQRIRDTRFRRLSYDGTWRENDLNRKLQSIEKKLPISRIKLAENKKDLSSNRHVETLKQKVMKLQKENEIRIPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GQS6</accession>
    <name>A0AAD5GQS6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_028226</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006396">
        <property type="protein sequence ID" value="KAI7749171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GQS6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7749171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="111" mass="12631" checksum="9CF847794CCB0BA5" modified="2024-05-29" version="1">MLDGRSDNSTRLLQPDRSRSLRHGSREMPEGLITHKYHMGCLHLSTFNCQDLTSLKVVEIEVLTTTQIQRNKIGTRLVQNRPTDGGIISILVPPKSRVTKLSMFDWISGNW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GRD2</accession>
    <name>A0AAD5GRD2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="9">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_015853</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the peptidase C19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="9"/>
    <dbReference type="EMBL" id="JAMZMK010005665">
        <property type="protein sequence ID" value="KAI7752502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GRD2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01795">
        <property type="entry name" value="Ubl_USP48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2230.10:FF:000005">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2230.10">
        <property type="entry name" value="DUSP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035927">
        <property type="entry name" value="DUSP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006615">
        <property type="entry name" value="Pept_C19_DUSP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044743">
        <property type="entry name" value="Ubl_USP48"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF722">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00695">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143791">
        <property type="entry name" value="DUSP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51283">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4 9" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="5 9" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="10">
        <location>
            <begin position="1"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="DUSP" evidence="11">
        <location>
            <begin position="326"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="domain" description="DUSP" evidence="11">
        <location>
            <begin position="555"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="913"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7752502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="913" mass="101852" checksum="47E1DB78F11EBBFE" modified="2024-05-29" version="1" fragment="single">MFEKPVCYPGCRVNTKDNPNCFCGLIPPPNGNRKTGLWQKTSEIVSSLGADPSKDLRDSLDTPAGLTNLGATCYANSILQFLYMNKSFREGVLSVEPEALEQQPVLNQIARLFAQLHASKMAFFLRPEALFKISFVETTTKKKITSAFSFPGQVDMARWLSEQSDSEMLYDLSAVLIHKGSAVNSGHYVAHIKDQDTGLWWEFDDELVSDLGQHPFGGSSSNMSTKPVQSCSAEPNSVVNGNHMDASSSGTNAHVQTFSSTDAYMLMYSLRNQTSGDRKPQMGSAECKLKDDAYLPPHLLKEVSELNKSYVESCEQYKIKKDAKLTLINERRQEVRSVLSEAVVQSPVESFWWISVDWLRQWADSIIPTQIDNTPIQCLHGKVPVSKISSMKRLSAKAWNTIFSKYEGGPALGEDDCCIECLMETARTTVSADSYRDGRTSMRELAELALAGKCSDGQLLSQWVRRRNVDLPCEADTGPTASIRCPHGSLMPEQAPGAKRALVPENLWLFIYKSANEVKPDDMIGCSVFPSDSDVCPQCCVELSEVTCMEDSLRDFKLKQRQSHEKLALGKTVSLSSENKYYLLPTSWLSKWRCYVTAVGKNASSTEEPEDLNAAIESLKCEQHSRLLHRPPSLVRKRGLILQKAPNVDELTIITEDDWTSFCKEWNCNEEKGIFAEIGLSNSVEDNLLGMSEDTPITEDNVNSLDDANGETESQGPTVKTYPEVCEDCIGERESCELVRKLNYCNEDICICFVRGKEPPKYILEGSGNLLEPNNRRISKRSRRTSYGNSVNLNVSGTTTLYELKMMIWQSFGIVKENQILHKGSKIVDGETATLADVCIFPGDVLWVTDSEIHENRDIAEELSDTKMEVQQSEGGDCKFMRIQGRLLMMFLPFLKSSTYDIPISGCVVVVAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GRE7</accession>
    <name>A0AAD5GRE7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Storage protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_030892</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006391">
        <property type="protein sequence ID" value="KAI7749248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GRE7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007877">
        <property type="entry name" value="DUF707"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31210:SF86">
        <property type="entry name" value="LYSINE KETOGLUTARATE REDUCTASE TRANS-SPLICING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31210">
        <property type="entry name" value="OS06G0731900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05212">
        <property type="entry name" value="DUF707"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7749248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="362" mass="42178" checksum="9855AF7B088AB846" modified="2024-05-29" version="1">MKIDEYKRRGSSIRRKPNESMKAIVTIAVGIVFGFYIGVSFPAFAPSKIFVSTNPRGAERLPPAIIASESDFYPRRLYGRPNEDLTLKPKYLVTFTVGYNQKENIDQTVKKFSDNFTILLFHYDGRTTEWDEFEWSKRAIHISVPKQTKWWYAKRFLHPDIIAPYEYIFIWDEDLGVENFDAEEYIKLVRKHKLEISQPGLDPSSKGLTWQMTMRREDLEVHKEAEEKPGWCNDRHLPPCAAFVEIMAPVFSREAWRCVWHLIQNDLVHGWGLDFALRKCIEPAYERIGVVDAQWIVHLGIPSLGNQGNEENGKTPGVRDRCKNEWKMFQERVANAEKDYYKSVGVGSMDHEANWVTPDRIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GVC2</accession>
    <name>A0AAD5GVC2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tubby-like F-box protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_007944</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the TUB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005546">
        <property type="protein sequence ID" value="KAI7753103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GVC2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22153">
        <property type="entry name" value="F-box_AtTLP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.50:FF:000047">
        <property type="entry name" value="Tubby-like F-box protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.90.10">
        <property type="entry name" value="Tubby Protein, Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025659">
        <property type="entry name" value="Tubby-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000007">
        <property type="entry name" value="Tubby_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018066">
        <property type="entry name" value="Tubby_C_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16517:SF86">
        <property type="entry name" value="TUBBY-LIKE F-BOX PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16517">
        <property type="entry name" value="TUBBY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01167">
        <property type="entry name" value="Tub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01573">
        <property type="entry name" value="SUPERTUBBY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54518">
        <property type="entry name" value="Tubby C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01200">
        <property type="entry name" value="TUB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01201">
        <property type="entry name" value="TUB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="5">
        <location>
            <begin position="53"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="Tubby C-terminal" evidence="4">
        <location>
            <begin position="114"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="305"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="376"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="315"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="376"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU361125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF12937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7753103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="443" mass="49250" checksum="19B291AC7A641AA9" modified="2024-05-29" version="1">MSFRSIVRDLRDGIGSLSRRGFEVRLSSLNKSKSQSAAHEIDDHLDFVTNSPWVGLPPELLSDVIKRLEASESSWPARKHVVACASVCRSWRNMCKEIVSSLESSGKITFPISLKQPGPRDPTIQCFIKRDKSNSTYYLYLCLSSALTVENGKFLLAAKRNRRSTCTEYIISMDADNISRSNNSYIGKVRSNFLGTKFIIYDSQPPHSTVAPPVRLGSSRRFSRKVSPKVPLGSYNIAQIAYELNVLGTRGPRRMQCVMNSIPMSALEPGGAIIPDHPNLIPPSLEDSFRSISFSKAIDNSSEFGSSRFPDMTDQPASTVNLQNGQKSMPLILKNKQPRWHEQLQCWCLNFKGRVTVASVKNFQLIAAIPPQVAVDPPLPPQTSQAQPEQTAQAQPPSSHDKVILQFGKVGKDIFTMDYRYPLSAFQAFAICLSSFDTKLACE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GW85</accession>
    <name>A0AAD5GW85_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_024583</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010003072">
        <property type="protein sequence ID" value="KAI7754541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GW85"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7754541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="92" mass="9958" checksum="229524D92BA85DBB" modified="2024-05-29" version="1" fragment="single">DISCINDMTMLYGAATIEDVYLAYEQLKIDEQSLYCCIGIAGAQAGDDDDDSGPLQEEEEYGGLPGVIPVGRGAWMKKTDHEIHSGDDSNDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GWU4</accession>
    <name>A0AAD5GWU4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_010371</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000671">
        <property type="protein sequence ID" value="KAI7755964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GWU4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7755964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="95" mass="10120" checksum="E17896A69E6DB991" modified="2024-05-29" version="1" fragment="single">REKGRIWNRPWPPASHTNPAYTTSLAVAKAVSGGGIAAKETLGIVDVDGPTREDLAIILEDLGINVDVVLKPNNIDIAPDFGRIGLQHRVAQIAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GZ29</accession>
    <name>A0AAD5GZ29_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_014449</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ARG7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000259">
        <property type="protein sequence ID" value="KAI7756778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GZ29"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009733">
        <property type="term" value="P:response to auxin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003676">
        <property type="entry name" value="SAUR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31929">
        <property type="entry name" value="SAUR-LIKE AUXIN-RESPONSIVE PROTEIN FAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02519">
        <property type="entry name" value="Auxin_inducible"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="9"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="24"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7756778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="133" mass="15009" checksum="330C3844A3BEC54C" modified="2024-05-29" version="1" fragment="single">PARISSRKTQTLETTTTTATQPTSLKDTLSSTLEKQEADTLKSHNKLTQILALKKNSNFRDDNAYPNDVPKGHFVVYVGETRSRYVIPISWLHHKGFQSLLQQAEEEFGFDHGMGLIIPCHEDDFLSLFSLMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BKM5</accession>
    <name>A0AAD5BKM5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Amino acid transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_026379</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the amino acid/polyamine transporter 2 family. Amino acid/auxin permease (AAAP) (TC 2.A.18.5) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012102">
        <property type="protein sequence ID" value="KAI7725010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BKM5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF692">
        <property type="entry name" value="TRANSMEMBRANE AMINO ACID TRANSPORTER FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="73"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="99"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="132"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="9">
        <location>
            <begin position="1"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7725010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="159" mass="17495" checksum="DB09F9030CEB2792" modified="2024-05-29" version="1" fragment="single">AVLGYMMFGESTESQFTLNLPTNLVASKIAVWATVISPLTKYPFYCETMIKPLALSLEELIPLKHQTTRIYSILIRTALVISTLLVALSIPFFGLLLSLIGSLLSMLVSLIFPCACFLRLLRGNITRFQGSLCWLTIAIGILSSAFGSYTALCKIIQLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BKV2</accession>
    <name>A0AAD5BKV2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_023953</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012030">
        <property type="protein sequence ID" value="KAI7725167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BKV2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000300">
        <property type="entry name" value="Myb transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015495">
        <property type="entry name" value="Myb_TF_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47994">
        <property type="entry name" value="F14D16.11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47994:SF5">
        <property type="entry name" value="F14D16.11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="HTH myb-type" evidence="7">
        <location>
            <begin position="17"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="6">
        <location>
            <begin position="17"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="77"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="149"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="85"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7725167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="171" mass="19592" checksum="77CD9B7B65131086" modified="2024-05-29" version="1" fragment="single">LLRCGKSCRLRWTNYLRPDLKRGLLSEYEEKMVIDLHAQLGNRWSKIASNLPGRTDNEIKNHWNTHIKKKLKKMGIDPVTHKSFAPSTLSTNHQSPPPPPTTPPLLAQPLHEAPQAEKVMVPQPMEYRNNEIKETYNDAISIQSSTITELSKDDYEEGEESKRSVVEFCTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BLS1</accession>
    <name>A0AAD5BLS1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cellulose synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_002217</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012180">
        <property type="protein sequence ID" value="KAI7724628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BLS1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016760">
        <property type="term" value="F:cellulose synthase (UDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030244">
        <property type="term" value="P:cellulose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005150">
        <property type="entry name" value="Cellulose_synth"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13301">
        <property type="entry name" value="X-BOX TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03552">
        <property type="entry name" value="Cellulose_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="88"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="121"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="157"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="202"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="247"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7724628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="292" mass="32433" checksum="BCBDFCD0D2FF8F33" modified="2024-05-29" version="1" fragment="single">VGWIYGSVTEDILTGFKMHCHGWRSVYCIPKRPAFKGSAPINLSDRLHQVLRWALGSVEILLSRHCPIWYGYGCGLKPLERLSYINSVVYPLTSLPLVAYCTLPAVCLLTGKFIVPEISNYASIIFLLMFLSIAVTGILEIQWGGVGIDDFWRNEQFWVIGGVSAHLFALFQGLLKVLAGVNTNFTVTSKGGDDGEFSELYVLKWTTLLIPPVTLLSLNIVGVVVGISDAISNGYESWGPLFGRLFFAIWVILHLYPFLKASVLTLLWVRLNPFVSKDGIVLEIETGRSNFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BM52</accession>
    <name>A0AAD5BM52_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Isopropylmalate dehydrogenase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_030515</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Performs an essential role in the oxidative function of the citric acid cycle.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011828">
        <property type="protein sequence ID" value="KAI7725917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BM52"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004449">
        <property type="term" value="F:isocitrate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006102">
        <property type="term" value="P:isocitrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.718.10:FF:000009">
        <property type="entry name" value="Isocitrate dehydrogenase [NAD] regulatory subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.718.10">
        <property type="entry name" value="Isopropylmalate Dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004434">
        <property type="entry name" value="Isocitrate_DH_NAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00175">
        <property type="entry name" value="mito_nad_idh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835">
        <property type="entry name" value="DECARBOXYLATING DEHYDROGENASES-ISOCITRATE, ISOPROPYLMALATE, TARTRATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835:SF42">
        <property type="entry name" value="ISOCITRATE DEHYDROGENASE [NAD] SUBUNIT BETA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53659">
        <property type="entry name" value="Isocitrate/Isopropylmalate dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0520">NAD</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Isopropylmalate dehydrogenase-like" evidence="12">
        <location>
            <begin position="87"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7725917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="415" mass="45435" checksum="31251FB7CF0870C4" modified="2024-05-29" version="1" fragment="single">FQLLKSQSPLHLCNYYSFPTCKHYSLNQNPNNPLSSFIFFTSLIKSNQTPMARRSLPILKQLLHGQHPPLSRSVTYMPRPGDGAPRTVTLIPGDGIGPLVTGAVEQVMEAMHAPIFFEKFEVHGDMKTIPTEVLESIRKNKVCLKGGLNTPMGGGVSSLNVQLRKELDLYASLVNCINFPGLVTRHENVDIVVIRENTEGEYAGLEHEVVPGVVESLKVITKFCSERIAKYAFEYAYLNNRKTVTAVHKANIMKLADGLFLESCREIASKYPSIKYNEIIVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGVMPGGNVGADHAVFEQGASAGNVGKQKFVERKTANPVALLLSSAMMLRHLQFPSFADRLENSVKHVILEGKYRTKDLGGNSTTQEVVDAVIANLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BNC0</accession>
    <name>A0AAD5BNC0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Phosphatidylinositol N-acetylglucosaminyltransferase subunit C</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_023730</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PIGC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011648">
        <property type="protein sequence ID" value="KAI7726500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BNC0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000506">
        <property type="term" value="C:glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009450">
        <property type="entry name" value="Plno_GlcNAc_GPI2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12982">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL GLYCAN, CLASS C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12982:SF0">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL N-ACETYLGLUCOSAMINYLTRANSFERASE SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06432">
        <property type="entry name" value="GPI2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016104">
        <property type="entry name" value="GPI2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="47"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="107"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="198"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="228"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="253"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7726500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="295" mass="33515" checksum="73B284D7B7D8E473" modified="2024-05-29" version="1">METTQRRWRKVAYGGMQPGFDDNHTDDTFLQEMVMNANVVKRDMLKVMLDSVSISQYLCIVALVVLVWAHTLNSTITENFLIILDITLVGSGFFILLLTAKMLSFKLLLSYAIKISFFTTGLYVLSPIYHTLTRSISSDSIWALTTSLVILHLFLHNYSGSTIKAPGALENPNLTSNISLNASIVASLLIASRLPSRLLVFAIILFSLQVFLFAPLVTYCLKKYSFRLHLMFSFVLMAITLTCVYRLHKLLFVVLLGLLVFVNLVCPYWLIRIQEYKFEINGPWDEAKLCFNITD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BNL7</accession>
    <name>A0AAD5BNL7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_000277</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011881">
        <property type="protein sequence ID" value="KAI7725676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BNL7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7725676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="86" mass="9830" checksum="B193AD016EF3D9C0" modified="2024-05-29" version="1">MPEGKRRRDLRGTYQEDVRFNPDHNMLIVHSVRTSIPLTSHCTCKASVQQKQASMVAFTGTSLLKKAIRKGQMTYENNPFFDLNMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BPS4</accession>
    <name>A0AAD5BPS4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">thioredoxin-disulfide reductase (NADPH)</fullName>
            <ecNumber evidence="3">1.8.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_023595</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.9" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010011395">
        <property type="protein sequence ID" value="KAI7727366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BPS4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004791">
        <property type="term" value="F:thioredoxin-disulfide reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd02949">
        <property type="entry name" value="TRX_NTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000064">
        <property type="entry name" value="Thioredoxin reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050097">
        <property type="entry name" value="Ferredoxin-NADP_redctase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008255">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_2_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48105">
        <property type="entry name" value="THIOREDOXIN REDUCTASE 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00469">
        <property type="entry name" value="PNDRDTASEII"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00573">
        <property type="entry name" value="PYRIDINE_REDOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="11">
        <location>
            <begin position="401"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="57"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="63"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7727366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="516" mass="56639" checksum="070883F30219F5D0" modified="2024-05-29" version="1">MVRNQPMASTPHITTGYPIRIGIPNSGRPSSSLLLRPRAAAFHSLLNFSAYRRSPPSCSLRASASSTEQPSTTTTTSSGQDVENLVIVGSGPAGYTAAIYAARANLKPVVFEGYQVGGVPGGQLMTTTEVENFPGFPDGITGPDLMDRMKRQAERWGAELHQEDVEFIDVNNRPFTVQSTDRATAKRLNLPREEEFWSRGISACAICDGASPLFKSQVLAVVGGGDTATEEAIYLTKYARHVHLLVRSGQMRASKAMQDRVLDNPNITVHYNTETVDVVSNTKGQMSGIKVKKLDTGEESVLEAKGLFYGIGHTPNSQLLQGQINLDSAGYILVKEGTAETSLEGVFAAGDVQDHEWRQAITAAGSGCIAALSVERYLVSNDLLIEFHQPHTEEVKKELTPRDVQEGFDITLTKHRGQYALRKLYHESPRLICVLYTSPTCGPCRTLKPILGKVIDEFDQNVHFVEIDIEEDPEVAEAANVMGTPCVQFFKNKEMIRSVSGVKMKKEYREFIEANK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BQ34</accession>
    <name>A0AAD5BQ34_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">type I protein arginine methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.319</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_018346</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-arginyl-[protein] + 2 S-adenosyl-L-methionine = N(omega),N(omega)-dimethyl-L-arginyl-[protein] + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:48096"/>
            <dbReference type="Rhea" id="RHEA-COMP:10532"/>
            <dbReference type="Rhea" id="RHEA-COMP:11991"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29965"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61897"/>
            <dbReference type="EC" id="2.1.1.319"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.319" evidence="1"/>
    <dbReference type="EMBL" id="JAMZMK010011666">
        <property type="protein sequence ID" value="KAI7726456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BQ34"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070611">
        <property type="term" value="F:histone H3R2 methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035242">
        <property type="term" value="F:protein-arginine omega-N asymmetric methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035241">
        <property type="term" value="F:protein-arginine omega-N monomethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000052">
        <property type="entry name" value="Probable histone-arginine methyltransferase CARM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.160.11">
        <property type="entry name" value="Hnrnp arginine n-methyltransferase1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025799">
        <property type="entry name" value="Arg_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057622">
        <property type="entry name" value="CARM1-like_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11006:SF10">
        <property type="entry name" value="HISTONE-ARGININE METHYLTRANSFERASE CARMER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11006">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25350">
        <property type="entry name" value="PH_PRMT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06325">
        <property type="entry name" value="PrmA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51678">
        <property type="entry name" value="SAM_MT_PRMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Probable histone-arginine methyltransferase CARM1-like N-terminal PH" evidence="7">
        <location>
            <begin position="10"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF25350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7726456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="370" mass="40686" checksum="3A0B2198527F0AB9" modified="2024-05-29" version="1">MNNTPPVENHSFTVASITLLESNPPTVVSGPLSAVFGSGSGFPELQFVVESVGQQFLGFDLRTTQLFRLGECQSLCVSEGSETVKERTYSKGITIQFKDEEESSSFHGAFEQWKSKVVIQGSRLPNGTVSSSKSKFDDKIEASSAKMYFDYYGQLLHQQNMMQDYVRTGSYYAAVMENQADFAGRVVVDVGAGSGILSLFAAQAGAKHVYAVEASEMADYAQKLIAGNPSLGQRITVIKGKVEDVELPEKADILISEPMGNLLINERMLEIHMAPFSDEYLYNELAKMSLFWQQQNYYGVDLTALYGTAFQGNFSQPVVDAFDPKLLVAPSVSHVINFTTVKEEDLYEINIPLKFTASVGTKIHALYKGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BQT2</accession>
    <name>A0AAD5BQT2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">phospholipase A2</fullName>
            <ecNumber evidence="5">3.1.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">M8C21_003601</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">AA19_3_7</strain>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1-acyl-sn-glycero-3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15801"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the phospholipase A2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.4" evidence="5"/>
    <dbReference type="EMBL" id="JAMZMK010011294">
        <property type="protein sequence ID" value="KAI7727923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BQT2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004623">
        <property type="term" value="F:phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050482">
        <property type="term" value="P:arachidonate secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04706">
        <property type="entry name" value="PLA2_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.90.10:FF:000005">
        <property type="entry name" value="Secretory phospholipase A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.90.10">
        <property type="entry name" value="Phospholipase A2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033113">
        <property type="entry name" value="PLA2_histidine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036444">
        <property type="entry name" value="PLipase_A2_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48619">
        <property type="entry name" value="Phospholipase A2, PLA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00118">
        <property type="entry name" value="PA2_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0106">Calcium</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 14" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041971667" description="phospholipase A2" evidence="14">
        <location>
            <begin position="18"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAI7727923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="154" mass="16632" checksum="605DE4635B76773F" modified="2024-05-29" version="1">MASFFLLLISILTLNYSSISLFALKVDLRADPGLSLSKECSKKCESSFCNGLLTVAPLLRYGKYCGLLYSGCPGEKPCDELDACCMKHDSCISANNNNYLSEVCNKGLLGCVERFKKAGSKTFKGNTCDVNDVTNTISAVMNAALLAGKYIQKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BRS2</accession>
    <name>A0AAD5BRS2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Polyadenylate-binding protein</fullName>
            <shortName evidence="11">PABP</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">M8C21_030015</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">AA19_3_7</strain>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Binds the poly(A) tail of mRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the polyadenylate-binding protein type-1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011211">
        <property type="protein sequence ID" value="KAI7728463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BRS2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12379">
        <property type="entry name" value="RRM2_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12380">
        <property type="entry name" value="RRM3_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12381">
        <property type="entry name" value="RRM4_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1900.10:FF:000003">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000003">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000217">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000239">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1900.10">
        <property type="entry name" value="c-terminal domain of poly(a) binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036053">
        <property type="entry name" value="PABP-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006515">
        <property type="entry name" value="PABP_1234"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002004">
        <property type="entry name" value="PABP_HYD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045305">
        <property type="entry name" value="RRM2_I_PABPs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01628">
        <property type="entry name" value="PABP-1234"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24012">
        <property type="entry name" value="RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00658">
        <property type="entry name" value="MLLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00517">
        <property type="entry name" value="PolyA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63570">
        <property type="entry name" value="PABC (PABP) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51309">
        <property type="entry name" value="PABC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0945">Host-virus interaction</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 10 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="RRM" evidence="13">
        <location>
            <begin position="15"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="13">
        <location>
            <begin position="106"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="13">
        <location>
            <begin position="209"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="PABC" evidence="14">
        <location>
            <begin position="456"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="373"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="378"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAI7728463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="553" mass="60282" checksum="F2E08820C906D794" modified="2024-05-29" version="1">MYSHRDPSVRKSGTANIFIKNLDKSIDNKALHDTFSSFGTILSCKIATDGTGQSKGYGFVQFETEEAAQTAIDKLNGMLMNDKQVYVGHFLRKQERDSSLSNAKFNNVYVKNLSESTTDEDLKKAFSEYGAITSAVVMRDADGKSKCFGFVNFENPDDAANAVEALNGKKIDEKEWYVGKAKKKSERELELKSRFEQTAKEAVDKFQGLNLYVKNLDDSVDDDKLKELFSEYGTITSCKVMRDPSGVSRGSGFVAFSSPEEASRALSEMNGKMIVSKPLYVALAQRKEERKAMLQAQFSQMRPIAMAPSMMAPRMPMYPPGAPGMGQQLFYGQPPPAMIPPQAGFGYQQQLVPGMRPGGGPMPNFFVPVVQQGQQGQRLGGRRGTGPGQQNQQSVPMMRPQMVPRGGMYRYPGRNVADANMAAGAGGGMLPVPYDMAGVGGMLPRDAAAPIPQPMPITALASALANASPEQQRTMLGENLYPLVDQLEHEHAAKVTGMLLEMDQTEVLHLLESPDALKAKVSEAMEVLKTVQQQANSPSAQMAALSLNENLVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BSC6</accession>
    <name>A0AAD5BSC6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glycosyltransferase</fullName>
            <ecNumber evidence="5">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_003855</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="5"/>
    <dbReference type="EMBL" id="JAMZMK010011356">
        <property type="protein sequence ID" value="KAI7727551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BSC6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008194">
        <property type="term" value="F:UDP-glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016138">
        <property type="term" value="P:glycoside biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000060">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008480">
        <property type="entry name" value="DUF761_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058980">
        <property type="entry name" value="Glyco_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48044">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48044:SF29">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05553">
        <property type="entry name" value="DUF761"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26168">
        <property type="entry name" value="Glyco_transf_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyltransferase N-terminal" evidence="6">
        <location>
            <begin position="9"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF26168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7727551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="460" mass="51501" checksum="E202F5320759EE04" modified="2024-05-29" version="1">MMNNHGRKLTILMFPWLGHGHISPFLELAKKLSNTNMFTIFLCSTPANLDFVKKTLKTEGGLCLSSVQLIELHLPTLPDLPSHLHTTNGLPIHLMPTLKQAFDMASQGFSKILKTLMPDLLIYDILQPWAPLAALALGIPSVVFITTSVAAAINTYHVECTEENCKDLGRVIACYNNSSSVILVKSFEEIEGKYFDYFSVLSSKKVVPVGPLVVDQCMDTTQNSVIEWLDRKAVRSTVFVSFGSEYFLSSDDLEEIAYGLEMSEVNFIWVLRFAKGQKEINVLEALPLGFVERVKNRGLIVEEWAPQTRILAHKNIGGFVSHCGWSSVMEAMMFGVPIIAMPMQFDQPVNARLVVEVGVGVEVSRDGNGRFKRENVAAFVQNVLVNKLGESVREKTKNMSVVLSVKGDKEIDVVVQELLRLCKRGGDDCQGEEEKEEEDSIDKRAERFIKKTYEEMRMQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BSK5</accession>
    <name>A0AAD5BSK5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_022644</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PHAF1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011133">
        <property type="protein sequence ID" value="KAI7728822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BSK5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005373">
        <property type="entry name" value="PHAF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039156">
        <property type="entry name" value="PHAF1/BROMI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13465:SF2">
        <property type="entry name" value="PHAGOSOME ASSEMBLY FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13465">
        <property type="entry name" value="UPF0183 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03676">
        <property type="entry name" value="PHAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00024339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7728822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="418" mass="47303" checksum="8964D341A8E21B2F" modified="2024-05-29" version="1">MGVIVLDLRPELRNDVLVAGMPICEAFAQIEQQPNIYDVVHVKYFDEDPLKLDVVISFPDHGFHLRFDPWSQRLRLIEIFDVKRLQMRYATSLIGGPSTPATFVAVYAVFGPTFPGIYDKDRGVYTLFYPGLSFAFPIPSQYTDCCHNGEAELPLEFPDGTTPVTCRVCIYDSSTDSKVGVGSLMNKASAPPLPAGSLYMEEVHVKLGEELWFTVGGQHIPFGASPQDVWTELGRPDGIHQKQVDQMVIHSASDPRPKTTLCGDYFYNYFGRGFDILFDGQTHKIKKFVLHTNYPGHADFNSYIKCNFVIHATESDSAYHQDMNSSKSSITPGTKWELVKFSHPVPLYRRFLEIVDELLFKHKALQAIHLARHLSTVTKMLRLRIALNNIADNGYVAINVEGKQWIYALYSSKMQHRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BWB5</accession>
    <name>A0AAD5BWB5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ion transport domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_015746</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010948">
        <property type="protein sequence ID" value="KAI7729653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005216">
        <property type="term" value="F:monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.630">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45651">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-GATED ION CHANNEL 15-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45651:SF11">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-GATED ION CHANNEL 20, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0407">Ion channel</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="74"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="159"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="237"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="366"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="8">
        <location>
            <begin position="74"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7729653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="592" mass="68056" checksum="CA3874B5DC2AEB7D" modified="2024-05-29" version="1">MKSGPLGMCSDPYCTTCPSSYYNKGRLVSRVPHGVDYHLHAYGGANRWVREKLSLLMQFTLGVMNPHAKFVQKWNQFVIISCLFAIFIDPVFFYLLSVKQEQKCIFINWPMTKTIVIFRSMTDFIYLLHMLVQFRLAFISPESRVVGAGDLVDDPKKIALHYLSGYFFLDLFVVLPLPQIIILLVLPNSISFGATHAQNLLRSAVLVQYIPRLYRFLPLLAGQSPTNFIFESAWANFVINLLTYVLAGHVVGSCWYLFGLQRVNLCLRDACRKSGFGNCTMFIDCGHGNDFGRFTADPTWKNWKQNVHASACFNEDDFQYGIYVKAVNLTAEKSITTRYVYSFFWGFQQISTLAGNQNPSYYVSEVLFTLGIIALGLLLFALLIGNMQNFLQGLGRRRLEKSLRRRDVEQWMSHRLLPEELKRKVRESERYNWAATQGVEEEKLMENLPEDXXXXAATFLRKMESIGEDGTQVPLSEGDVCGEELLTWLDHSSFHGDTREQRKAGQVASKRTVTCLTNVEAFILKAADLEEVTTLFATFLSNHRVQMAIKNESPYWRSRAATTIQVAWRYWKKRRNRAHSGYTHPSNRLRYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BWD7</accession>
    <name>A0AAD5BWD7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_023036</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010682">
        <property type="protein sequence ID" value="KAI7730672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BWD7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032436">
        <property type="term" value="P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14939">
        <property type="entry name" value="F-BOX ONLY PROTEIN 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14939:SF5">
        <property type="entry name" value="F-BOX ONLY PROTEIN 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="10"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7730672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="266" mass="28936" checksum="B37A7C7AFB1EDF7A" modified="2024-05-29" version="1" fragment="single">MATTIDIIGEDLLHNIVSRLPATSFAYAACVSRSWNLVCERVLSRPKLASACSFNPNFDDVVIEVLNKVLSQPIRPHFAIVSLGGSYEPDDDSDDEFEVLEEALDLITAALGSNVQVVANRPSGIIGRDEFSDEIKKIELGFGEENDSILLIVGFVPGLKVTTIPLAKPFEVFMHQGPETAMIDEFITNIREFSTSVSGCNSPAAIIMFADIDACMIAVMEKMDYAMSPETVIVGDALSRFRCNNEAQDGISAAVALVFAVDRNKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BYX0</accession>
    <name>A0AAD5BYX0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_002502</name>
        <name evidence="9" type="ORF">M8C21_008103</name>
    </gene>
    <organism evidence="9 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Phosphate:H(+) symporter (TC 2.A.1.9) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010261">
        <property type="protein sequence ID" value="KAI7732303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAMZMK010006846">
        <property type="protein sequence ID" value="KAI7747066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BYX0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511:SF44">
        <property type="entry name" value="MAJOR FACILITATOR, SUGAR TRANSPORTER, MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511">
        <property type="entry name" value="SYNAPTIC VESICLE GLYCOPROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="26"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="26"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7732303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7747066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="91" mass="9974" checksum="C09D5247CE9771E1" modified="2024-05-29" version="1">MTDQTSTYTLDEALVAIGFGKFQGWLIVYAGLGSIAEAMEVMILSFIGPSVKSEWNLSSTQESLITTVVFAGMLIGAYSWGFISDNYGRRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C1D3</accession>
    <name>A0AAD5C1D3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_022151</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010075">
        <property type="protein sequence ID" value="KAI7733155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C1D3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010333">
        <property type="term" value="F:terpene synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.130">
        <property type="entry name" value="Terpene synthase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001906">
        <property type="entry name" value="Terpene_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036965">
        <property type="entry name" value="Terpene_synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050148">
        <property type="entry name" value="Terpene_synthase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005630">
        <property type="entry name" value="Terpene_synthase_metal-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31225">
        <property type="entry name" value="OS04G0344100 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31225:SF196">
        <property type="entry name" value="TERPENOID CYCLASES_PROTEIN PRENYLTRANSFERASE ALPHA-ALPHA TOROID-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01397">
        <property type="entry name" value="Terpene_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03936">
        <property type="entry name" value="Terpene_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Terpene synthase N-terminal" evidence="5">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="Terpene synthase metal-binding" evidence="6">
        <location>
            <begin position="181"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7733155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="257" mass="30390" checksum="05136A0E17D713B5" modified="2024-05-29" version="1" fragment="single">LINDIQRLGIAYCFEQEIVKDLGHIYTIYGDDWTDDSNSLWFRLLRQQGFYVSCDIFNRYKDNEGSFKEYVSRDVQGMLELYEAAYLRVRGEVVLDDALVYTKSQLEKITKGPLQWYCSLSLLKGGSRAAYMEKVSMGRRSAIHNFYEQQDSHNESLLRLAKWWKAIDPPKNLHYIRARIPRYSRSRIFLTKIIKMATLLDDTYDFYGTYEELEIITEAPTYTWSITCIDTLPLPDYMKLTYKTIRLLRRNRVNLGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C2M9</accession>
    <name>A0AAD5C2M9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Angiotensin-converting enzyme 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_018663</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009929">
        <property type="protein sequence ID" value="KAI7733685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C2M9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006476">
        <property type="entry name" value="CHP01589_pln"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01589">
        <property type="entry name" value="A_thal_3526"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31871">
        <property type="entry name" value="OS02G0137100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31871:SF61">
        <property type="entry name" value="OS06G0705300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09713">
        <property type="entry name" value="A_thal_3526"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7733685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="81" mass="9258" checksum="7ACF99ACD932F7AE" modified="2024-05-29" version="1">MADSSASYIHMVHHLIEKCLIFNMSKEECMEALSKHANIMPAITSTVWSELEKENKDFFESYNNTRRDHQTQSPSSSSSSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C3V7</accession>
    <name>A0AAD5C3V7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_012188</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009766">
        <property type="protein sequence ID" value="KAI7734285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C3V7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7734285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="242" mass="27418" checksum="0AE8C60CEB0970D3" modified="2024-05-29" version="1" fragment="single">KIDKISLCKAKLRLFLTKKNGFKVLNLYISAIRTQKFTDIEKVKAIELPPYELEHVELQLDTLEESSAHIAFVDAVLWCCHPLSLTLRSSVPFEEHGDLVKFTYKKLLEQEDHTSIQIVGPSSSEAQKQLRDLKSLSVGLSRDGNAISFIKEEVVQEEALSQRSLVMLSVKTKFTYKKLLEQEDHTSIQIVGPSSSEAQKQLRDLKSLSVGLPRDGKAISFIKERVVQEEALGQRFADMEIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C534</accession>
    <name>A0AAD5C534_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">glycerophosphodiester phosphodiesterase</fullName>
            <ecNumber evidence="1">3.1.4.46</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_017884</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a sn-glycero-3-phosphodiester + H2O = an alcohol + sn-glycerol 3-phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12969"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:83408"/>
            <dbReference type="EC" id="3.1.4.46"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.46" evidence="1"/>
    <dbReference type="EMBL" id="JAMZMK010009476">
        <property type="protein sequence ID" value="KAI7735526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C534"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008889">
        <property type="term" value="F:glycerophosphodiester phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006071">
        <property type="term" value="P:glycerol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046475">
        <property type="term" value="P:glycerophospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051578">
        <property type="entry name" value="GDPD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030395">
        <property type="entry name" value="GP_PDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22958:SF34">
        <property type="entry name" value="GLYCEROPHOSPHODIESTER PHOSPHODIESTERASE GDPD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22958">
        <property type="entry name" value="GLYCEROPHOSPHORYL DIESTER PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03009">
        <property type="entry name" value="GDPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51704">
        <property type="entry name" value="GP_PDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0319">Glycerol metabolism</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GP-PDE" evidence="5">
        <location>
            <begin position="1"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7735526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="309" mass="35102" checksum="F574C72C7B25329E" modified="2024-05-29" version="1" fragment="single">HVNSDDLNHTTNVAALNPSHHHNYNPHDHDDIGDTVVVNNTDKKFMGVISEKRVTDMNLDEFLSYGPQRDPAKVGKPLFRKTKDGRIFEWKVEKDDHLCTLEEMFQKINHSKGFNIEFKFDDNIVYKEEELVHVIQVVLCIVFKYARNRSIFFSSFQPDVALLTRKLQSTYPVFFLTNGGVETYTDVRRNSLDEAMKLCLAGGLNGIVCEVRSILRNPGVIKRIKDLKLSLISYGQLNNVGEVVNAQILMGVDGVIVDLVQEIIEVVADFGKEVEKKSIVEEENDDGFKCSEKQLSSLMKVIGMKYICS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C5P1</accession>
    <name>A0AAD5C5P1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_017110</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009434">
        <property type="protein sequence ID" value="KAI7735826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C5P1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000095">
        <property type="entry name" value="protein STRUBBELIG-RECEPTOR FAMILY 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000125">
        <property type="entry name" value="Protein STRUBBELIG-RECEPTOR FAMILY 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47989:SF45">
        <property type="entry name" value="OS01G0709500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47989">
        <property type="entry name" value="OS01G0750732 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="127"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7735826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="418" mass="45636" checksum="5637B5A32DA3C4BF" modified="2024-05-29" version="1" fragment="single">DGGKKSGVNGGAIAGIVISVLVVGALIAFFLLKKKSRKSSHTDIEKMENQPFASLASQPQELKSVQAFQIPPAVNLKPPPMERHKSFVDDDDDNISAKPVVPKKISVAPTNVISYSIADLQIATNSFSADNLIGEGSTGRVFRAQFEDGKVVAVKKIKPSALGAGENFIDIVSDISRLRHPNVTELVGYCCEHGQRLLVYEFLKNGSLYDFLHLSDEYSCKPLTWNNRVKIALGAARALEYLHEVCSPSVIHKSINSTNILLDSELNPHLSECGLASHVPDAEQELDSSAPEVSMSGVYTVKSDVYGFGVTMLELLTGRKPFDSSRTRSEQSLVRWATPQLHDIDALAKMVDPALKGLYPVKSLSRFADVIALCVQPEPEFRPPMSEVVEALVRLVQRTNMSKRTVGNDVRPDSEGEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C7P0</accession>
    <name>A0AAD5C7P0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 15">Glucose-1-phosphate adenylyltransferase</fullName>
            <ecNumber evidence="7 15">2.7.7.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">ADP-glucose pyrophosphorylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">M8C21_025518</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">AA19_3_7</strain>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 15">This protein plays a role in synthesis of starch. It catalyzes the synthesis of the activated glycosyl donor, ADP-glucose from Glc-1-P and ATP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>alpha-D-glucose 1-phosphate + ATP + H(+) = ADP-alpha-D-glucose + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:12120"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57498"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="EC" id="2.7.7.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4 15">Glycan biosynthesis; starch biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6 15">Heterotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 15">Plastid</location>
            <location evidence="3 15">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 15">Belongs to the bacterial/plant glucose-1-phosphate adenylyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.27" evidence="7 15"/>
    <dbReference type="EMBL" id="JAMZMK010009279">
        <property type="protein sequence ID" value="KAI7736394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C7P0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008878">
        <property type="term" value="F:glucose-1-phosphate adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005978">
        <property type="term" value="P:glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019252">
        <property type="term" value="P:starch biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02508">
        <property type="entry name" value="ADP_Glucose_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04651">
        <property type="entry name" value="LbH_G1P_AT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000030">
        <property type="entry name" value="Glucose-1-phosphate adenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011831">
        <property type="entry name" value="ADP-Glc_PPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005836">
        <property type="entry name" value="ADP_Glu_pyroP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02091">
        <property type="entry name" value="glgC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002772">
        <property type="entry name" value="PRK02862.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43523:SF12">
        <property type="entry name" value="GLUCOSE-1-PHOSPHATE ADENYLYLTRANSFERASE LARGE SUBUNIT 1, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43523">
        <property type="entry name" value="GLUCOSE-1-PHOSPHATE ADENYLYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25247">
        <property type="entry name" value="LbH_GLGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="Trimeric LpxA-like enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00808">
        <property type="entry name" value="ADP_GLC_PYROPHOSPH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00809">
        <property type="entry name" value="ADP_GLC_PYROPHOSPH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00810">
        <property type="entry name" value="ADP_GLC_PYROPHOSPH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="13 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 15" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="12 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11 15" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="15" id="KW-0934">Plastid</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14 15" id="KW-0750">Starch biosynthesis</keyword>
    <keyword evidence="10 15" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleotidyl transferase" evidence="17">
        <location>
            <begin position="81"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU362093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAI7736394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="512" mass="56626" checksum="1CCA50B4C8DE6147" modified="2024-05-29" version="1">MAVSLSTTLVRQPHGGSGLASRDWRPVNGELMGNKLSFSQKTQKKNNVKTSICMTLTGIATGEAKLRDLDMERRDPRTVVAVILGGGAGTRLFPLTKRRAKPAVPIGGAYRLIDVPMSNCINSGINKVYILTQFNSASLNRHLARAYNFGNGVTFGDGFVEALSATQTPGEEGKRWFQGTADAVRQFHWLFEDARSKDIEDVLILSGDHLYRMDYMDFLQSHRQSGADITISSLPMDDSRASDFGLMKIDGKGRVVSFSEKPKGNELKAMAVDTTVLGLSKEEAVKKPYIASMGVYIFKKEILLNLLRWRFPTANDFGSEIIPASAQEYHIKAFLFNDYWEDIGTIRSFFEANLALTEHPSRFSFYDAAKPMYTSRRNLPPSNVDNCKITDSILSHGSYLTNSFIEHSVVGIRSRIGSNVHLKDTVMLGADYYETDAEMKSLLAEGRVPIGIGENTKITTCIIDKNARIGKNVIIANSEGIQEADRSSEGFYIRSGVTIILKNSTIKDGLVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C952</accession>
    <name>A0AAD5C952_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">MLO-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">MLO</name>
        <name evidence="10" type="ORF">M8C21_008998</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">May be involved in modulation of pathogen defense and leaf cell death.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="8">The C-terminus contains a calmodulin-binding domain, which binds calmodulin in a calcium-dependent fashion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the MLO family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009096">
        <property type="protein sequence ID" value="KAI7737159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C952"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004326">
        <property type="entry name" value="Mlo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31942">
        <property type="entry name" value="MLO-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31942:SF49">
        <property type="entry name" value="MLO-LIKE PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03094">
        <property type="entry name" value="Mlo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="6 8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 8" id="KW-0568">Pathogenesis-related protein</keyword>
    <keyword evidence="4 8" id="KW-0611">Plant defense</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="18"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="60"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="150"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="273"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="301"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="357"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="398"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU280816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7737159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="562" mass="64036" checksum="8F867741760A1F7D" modified="2024-05-29" version="1">MAGDTSEGTRQLDQTPTWAVAGVCALFIIVSIALEKIMCKVGKFLTDKHKKALFEALEKVKAELMILGFISLMLTFSPYYIAKICIPVDVADTMLPCAKRHKAEKEEEEEKARRLLLWYEEHRRSLAGASKTICKKGKVPIITVDGMHQLHILVFFLAVIHVVYSVITMALGKLKARGWKQWEEETLSPDYEFTNDPSRFRLTHQTSFVRAHTSFWTRLPFFFYIGCFFRQFFRSVSKSDYMTLRNGFINLHLAPGTRFNFQKYLKRSLEDDFRVVVGVSPVLWASFVIFLLLNVNGWQTLFWASLLPLVIILAVGTKLQAILTKMALEITETYAVVQGIPLVQASDKYFWFSRPRLMLHLIHFALFQNAFQLTYFFWICYEYSINSCFHDNIRLVIVKLAIGVGVLILCSYTTLPLYALLSQMGSHLKKSIFDEQIAKALKKWRMAVKKKRGGKSLGRSTTSSVTASPVHSMASTIYPAVGATLHRFKTTGHSTRSFTYEDTDASDLEAEPLTPKDPSAHLLDVQVDYHSDNEIELAESSQQVVTRNEDEFSFVKTAAPME</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C9E5</accession>
    <name>A0AAD5C9E5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Biotin carboxyl carrier protein of acetyl-CoA carboxylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_008845</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">This protein is a component of the acetyl coenzyme A carboxylase complex; first, biotin carboxylase catalyzes the carboxylation of the carrier protein and then the transcarboxylase transfers the carboxyl group to form malonyl-CoA.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Plastid</location>
            <location evidence="7">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008996">
        <property type="protein sequence ID" value="KAI7737542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C9E5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009317">
        <property type="term" value="C:acetyl-CoA carboxylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003989">
        <property type="term" value="F:acetyl-CoA carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06850">
        <property type="entry name" value="biotinyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000003">
        <property type="entry name" value="Acetyl-CoA carboxylase biotin carboxyl carrier protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050537">
        <property type="entry name" value="2-oxoacid_dehydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001249">
        <property type="entry name" value="AcCoA_biotinCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001882">
        <property type="entry name" value="Biotin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00531">
        <property type="entry name" value="BCCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43416:SF35">
        <property type="entry name" value="BIOTIN CARBOXYL CARRIER PROTEIN OF ACETYL-COA CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43416">
        <property type="entry name" value="DIHYDROLIPOYLLYSINE-RESIDUE SUCCINYLTRANSFERASE COMPONENT OF 2-OXOGLUTARATE DEHYDROGENASE COMPLEX, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01071">
        <property type="entry name" value="ACOABIOTINCC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00188">
        <property type="entry name" value="BIOTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0092">Biotin</keyword>
    <keyword evidence="7" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="5 7" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="3 7" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2 7" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="4 7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-0934">Plastid</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="9">
        <location>
            <begin position="203"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="78"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="166"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="186"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7737542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="273" mass="28916" checksum="C08B20F129C629CF" modified="2024-05-29" version="1" fragment="single">MASFTVNCPKTSPFFTAQPTPNQNPNHRNNSVVAFQSGFKSNRALIAGSSSSLGFQSSKRGQNDVTRVSAQLNQTALEQSLNSAPASKKSEESTKISSPDATVPDAASITAFMNQVAELVQLVDSRDIMELELKQQNCEVVIRKKEALPQPPPGPMVMMQSPQPQAIYQSLPPPPQSAPASSGPAPSAPALPSPTPAKPKSSHPPMKCPMAGTFYRSPAPGAPPFVKVGDKVQKGQVICIIEAMKLMNEIEADQSGTIVDILAEDGKPVSLDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CAJ7</accession>
    <name>A0AAD5CAJ7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">LIM zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_032159</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008927">
        <property type="protein sequence ID" value="KAI7737895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CAJ7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051017">
        <property type="term" value="P:actin filament bundle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24206">
        <property type="entry name" value="OS06G0237300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0440">LIM domain</keyword>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="5">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7737895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="64" mass="7468" checksum="B27C55DB710BA765" modified="2024-05-29" version="1" fragment="single">MLVTRLVIYHKRCFKCSHCKGTLVMSNYSSMDGVLYCMPHFEQLFKETGNFSKNFPSKPNRDNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CBN4</accession>
    <name>A0AAD5CBN4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PGG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_030393</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008809">
        <property type="protein sequence ID" value="KAI7738479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CBN4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026961">
        <property type="entry name" value="PGG_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24177:SF475">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING DOMAIN, PGG DOMAIN PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24177">
        <property type="entry name" value="CASKIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13962">
        <property type="entry name" value="PGG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="794"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="835"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="423"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="459"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="domain" description="PGG" evidence="3">
        <location>
            <begin position="783"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="859"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7738479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="859" mass="98807" checksum="F03EFDFEEE03E951" modified="2024-05-29" version="1" fragment="single">MEYMQLKSNVNLSNFVSVKLSGHNNYRIWKDQMRHLLQSHSLLHIIDEEARSPQDKDDPMTKKYNRLVRDETEDLKYMEASNVNVSNFVSVNLSNDSNYSIWRAQMLYLLESHSLLHIITDIHGFEKVKYHKLVNGWILRTMNDELLKEFPAEDYIYIDYSANYLWEELESRFTKGYSRPEPIVSASTQDLKYTQALNVNVSNFVTAKLSGRSNYRIWKGQMLCLIESQRLLHIIDKEGGYEVGKYDTLVKGWIFSTMNEQLLSDLTFTDDLVGGYNTAKDVWEKLTSAFDRQDAQEVEFGIEFVKDDTKSENDLLESDNTDKKRWYKAVAEGCWKKARSIFKVNKNVATEATTANENTNFHVAVERESSGTEVVRDTPDVETGNSRIEVKELYEAAAKGRWRKAKSILRNSETASIEAITTNGNTILHVAVERGQNHFVEELLGFLKNGEDIEKKNEKGRTALHIAAMVGNTNATQLLVQKREELLGIEDDDKNLPLHLASEYMNLNIYAYLVKQSLRPSYSDFRTYLSHRATIVPAIFSKQYDLAETLLKDLEEIMLKRFPMLPNSKRFRPYEEILMAITISYPTDIGFMESFIYPSFQNVCQKTIVRVRPIKNIEKKKKEYKDAKRILSLMRKSNIVHNDSLFEAVRLDIHEVVDEILFTSPATINCKDEEGYNIIQSSILNRAEKVYNLIYHIIERTKSHREMTDSFSNNLGHLCGKLAPSFVLGRTTGAALQLQRELIWFKWRSSCYLYNLRKKNIYKETPAMVFTREHRDLLKQGETWMKTTAESCSITAALIVTVVFAAAITVPGGSNQESGIPLFKKDIAFTIFARPWMLAPISAFACLPISVIATIKLPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CDD9</accession>
    <name>A0AAD5CDD9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_010761</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in the transport of sterols.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the OSBP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008628">
        <property type="protein sequence ID" value="KAI7739418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CDD9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13294">
        <property type="entry name" value="PH_ORP_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.3490:FF:000013">
        <property type="entry name" value="Oxysterol-binding protein-related protein 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037239">
        <property type="entry name" value="OSBP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000648">
        <property type="entry name" value="Oxysterol-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972:SF96">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN-RELATED PROTEIN 1A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01237">
        <property type="entry name" value="Oxysterol_BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144000">
        <property type="entry name" value="Oxysterol-binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="6" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PH" evidence="8">
        <location>
            <begin position="78"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="295"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="321"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="351"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="369"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="392"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7739418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="487" mass="55117" checksum="E5E843C14B3C6169" modified="2024-05-29" version="1">MHPFCCVNADHNNNSMPATPSSSAVIRSEFNNHHIIHPNPNPNVNSYNNNHHVAFNTVSGGDQRDVLITSTVNDIVGNNGISGVLNKWVNYGKGWRPRWFVLQDDGVLSYYKFHGPHKIVFSGEDVKGARVIGDESFRRLSRMSSGSHVVGSQHRKPVGEVHLKVSSIRESSSDDKRFSIFTGTKRLHLRADTREDRIAWMEALQAVKGMFPRMSNSELMASISGITVTTEKLRQRLLEEGVSEAAIQDSENIMRSEFSALQDQLVLLKQKHLLLMDTLRMLETEKVDLENTVVDESQRQSKEVGATSRLRQEKCSASPSESEDDNERGDAAEEESDDEDNAFFDTRDFLSSGSFKSSGSDMMSPSFTSDDEDLQTNEPEIDASIRSVGTDFPREKLPPTDSRLRPDQRCLENGEYEMANAEKLRLEQRQRQAKKMQERGWKPRWFAKDKASDTFVYTGGYWEAREQAKWDSCPDIFGQFSTDQTSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CFU6</accession>
    <name>A0AAD5CFU6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Actin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_020147</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the actin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008341">
        <property type="protein sequence ID" value="KAI7740827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CFU6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051286">
        <property type="term" value="C:cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030427">
        <property type="term" value="C:site of polarized growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048767">
        <property type="term" value="P:root hair elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd10224">
        <property type="entry name" value="ASKHA_NBD_actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000131">
        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000291">
        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000001">
        <property type="entry name" value="Actin, muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000058">
        <property type="entry name" value="Putative actin-related protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020902">
        <property type="entry name" value="Actin/actin-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004001">
        <property type="entry name" value="Actin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00190">
        <property type="entry name" value="ACTIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00406">
        <property type="entry name" value="ACTINS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00432">
        <property type="entry name" value="ACTINS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01132">
        <property type="entry name" value="ACTINS_ACT_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7740827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="376" mass="41627" checksum="F26CF2B127F516C7" modified="2024-05-29" version="1">MAEEDIQPLVCDNGTGMVKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDAYVGDEAQSKRGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNAPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDSLMKILTERGYSFTTSAEREIVRDMKEKLAYIALDFEQELETSKTSSSVEKSFELPDGQVITIGAERFRCPEVLFQPSMIGMESAGIHETTYNSIMKCDVDIRKDLYGNIVLSGGTTMFPGIADRMSKEITALAPSSMKIKVVAPPERKYSVWIGGSILASLSTFQQMWIAKAEYDESGPSIVHRKCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CFY9</accession>
    <name>A0AAD5CFY9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_000512</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008574">
        <property type="protein sequence ID" value="KAI7739746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CFY9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005504">
        <property type="term" value="F:fatty acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009627">
        <property type="term" value="P:systemic acquired resistance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00010">
        <property type="entry name" value="AAI_LTSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.110.10">
        <property type="entry name" value="Plant lipid-transfer and hydrophobic proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036312">
        <property type="entry name" value="Bifun_inhib/LTP/seed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016140">
        <property type="entry name" value="Bifunc_inhib/LTP/seed_store"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039265">
        <property type="entry name" value="DIR1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33122">
        <property type="entry name" value="LIPID BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33122:SF4">
        <property type="entry name" value="OS04G0415800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14368">
        <property type="entry name" value="LTP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47699">
        <property type="entry name" value="Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042066106" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein" evidence="1">
        <location>
            <begin position="24"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical" evidence="2">
        <location>
            <begin position="40"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7739746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="118" mass="12173" checksum="35552CADD6200E13" modified="2024-05-29" version="1">MEGSMKNMCLLGLLLALVIAGLGEVNGAHECGRADPNMEAFKLAPCASAAQDENASVSGSCCAAVRKLGQNPKCLCAVMLSNTAKASGVKPEIAMTIPKRCNIADRPVGYQCGAYTLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CGF1</accession>
    <name>A0AAD5CGF1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Bifunctional aspartate aminotransferase and glutamate/aspartate-prephenate aminotransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_023858</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="7">Amino-acid biosynthesis; L-phenylalanine biosynthesis; L-arogenate from prephenate (L-Asp route): step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Amino-acid biosynthesis; L-phenylalanine biosynthesis; L-arogenate from prephenate (L-Glu route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008234">
        <property type="protein sequence ID" value="KAI7741248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CGF1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033853">
        <property type="term" value="F:aspartate-prephenate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033854">
        <property type="term" value="F:glutamate-prephenate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004069">
        <property type="term" value="F:L-aspartate:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009095">
        <property type="term" value="P:aromatic amino acid family biosynthetic process, prephenate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000033">
        <property type="entry name" value="Aspartate aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050596">
        <property type="entry name" value="AspAT/PAT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46383">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46383:SF1">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="9">
        <location>
            <begin position="107"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00060544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00060601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00074369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7741248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="482" mass="51888" checksum="A4BF3B7A77DD9C5D" modified="2024-05-29" version="1">MASHLSGISFTYPTHSSITDQSSGTTSFRSTTLSFSSTLNSTLSISRSLETRRHHKSSTVCSAVILDSHSEPMEVDITLSPRVNSVKPSKTVAITDQATALVEAGVPVIRLAAGEPDFDTPAVIAEAGINAIREGYTRYTPNAGTLELRTAICKKLKEENDLSYTPDQIVVSNGAKQSILQAVLAVCSPGDEVIIPAPFWVSYPEMARLADATPVILPASISENFLLDPKLLESKINEKSRLLILCSPSNPTGSVYPKQLLEEIARVVAKHPRLLVLSDEIYEHIIYAPAVHTSFASLPGMWERTLTVNGFSKAFAMTGWRLGYIAGPKHFVAACNKIQSQSTSGASSIAQKAAVAALGMGHAGGEAVSQMVKAFRERRDFLVSAFGELDGVKMSEPQGAFYLFLDFSYYYGTEVEGFGLINDSESLCRYFLEQGQVALVPGDAFGDDTCIRISYAASLSTLQAAAERIKKAILTLKSPASV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CGS8</accession>
    <name>A0AAD5CGS8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">LYR motif containing domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_000184</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008186">
        <property type="protein sequence ID" value="KAI7741488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CGS8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056185">
        <property type="entry name" value="LYRM_2_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36724">
        <property type="entry name" value="COMPLEX 1 LYR-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36724:SF1">
        <property type="entry name" value="COMPLEX 1 LYR-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23655">
        <property type="entry name" value="LYRM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LYR motif containing" evidence="1">
        <location>
            <begin position="12"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF23655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7741488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="90" mass="10269" checksum="86D768B78C7800D0" modified="2024-05-29" version="1">MSKGLIWATAEDLSRNRGRILSLYRQVLRALNSPDLPLNLAARLHRKAQARAIFMVAAEERSIHNIKDLIDVAEYSLSLLRKGEIPKYIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CHY4</accession>
    <name>A0AAD5CHY4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Auxin response factor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_021969</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ARF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008327">
        <property type="protein sequence ID" value="KAI7740869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CHY4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009734">
        <property type="term" value="P:auxin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.1040:FF:000001">
        <property type="entry name" value="Auxin response factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010525">
        <property type="entry name" value="ARF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044835">
        <property type="entry name" value="ARF_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31384:SF5">
        <property type="entry name" value="AUXIN RESPONSE FACTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31384">
        <property type="entry name" value="AUXIN RESPONSE FACTOR 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06507">
        <property type="entry name" value="ARF_AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0927">Auxin signaling pathway</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Auxin response factor" evidence="8">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF06507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7740869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="260" mass="29315" checksum="60B0FD32A4664510" modified="2024-05-29" version="1">MRFNLRFETEDATERRCRGIITGISDVDPVNWPGSKWKSLMVKWDGVEAEAARRNRVSPWEIELQNSASGSGFDSILSPVSKRIKTDFRPEFQVPEDGPAGPLDFNKSLRFQKVLQGQEIFSHDHCPYGIIGSRNSMISLLRNDLRNRAIQASEVLQGQEIVCTKSMHPPSVIRFPNVILQPTYPRPINVANDSRHPDFSNGYLGQHNSHNQQETVSSLLTKKSLCLNPNPTLKQSHIGPQESVSSCRSSCRLFGFTLRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CIN3</accession>
    <name>A0AAD5CIN3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PAP/OAS1 substrate-binding-related domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_002477</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008033">
        <property type="protein sequence ID" value="KAI7742289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CIN3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05402">
        <property type="entry name" value="NT_PAP_TUTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1410.10:FF:000013">
        <property type="entry name" value="PAP/OAS1 substrate-binding domain superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058920">
        <property type="entry name" value="PAP-OAS1-bd-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058921">
        <property type="entry name" value="PAP/OAS1-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45979:SF30">
        <property type="entry name" value="NUCLEOTIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45979">
        <property type="entry name" value="PAP/OAS1 SUBSTRATE-BINDING DOMAIN SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26180">
        <property type="entry name" value="PAP-OAS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81631">
        <property type="entry name" value="PAP/OAS1 substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PAP/OAS1 substrate-binding-related" evidence="2">
        <location>
            <begin position="100"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="295"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="396"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="536"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="645"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="792"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="935"/>
            <end position="1080"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="305"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="405"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="558"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="573"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="614"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="646"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="708"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="832"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="949"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="994"/>
            <end position="1033"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF26180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7742289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="1080" mass="119973" checksum="C964E2E87985BD54" modified="2024-05-29" version="1">MVFTFGSVPLKTYLPDGDIDLTAFSSNSNLKDSWATEVCDLLQAEEKNENAEFHVKEVQYIQAEVKIIKCLVENIVVDISFNQLGGLCTLCFLEEVDNLINQNHLFKRSIILIKAWCYYESRILGAHHGLISTYALETLVLYIFHLFNNSFAGPLEVLYRFLEFFSKFDWDNFCVSLWGPVPVDSLPDVTAEPPRKDSEELLLNKVFLDACSSVYAVFPNGQDNHGQPFLSKHFNVVDPLRVSNNLGRSVSKGNFFRIRSAFALGAKRLAKFLDCPKENLVAEVNKFFTNTWDRHGSGIRPDAPVTNSTALKIDTKMSTSGGKKSESVGQESDFDHTHSTDNQSESSPKARVATPGHANQGQRNLKSGLLANDIQRSPFARTRSSPELTDTYKYVPFQGTINKPPENEKTRNRRKNVETEVEKNEASSLHHVPHKSFEPSTESSIYHLEDMQQQEQDLVNMMASSAFDGFNGPVNLASGHLPFPFSPSFLASLGYTQRNMTGMIPSNMPFIDPQFAGMHFPHGLVPHYFPGVNVTSNSEDPVNEGRLGSAETNSGDENYNHEIWREADRVSSEIVSQDENKSESSSPRMNYIPPPRRVGGSGALVRSQHKNNKEKRDRLRENNHLDYSGSLEYKLNDAYSDERATSSRFSSAAHSSSARSKTSSESSWDETSTVNQSTKEKKGKKTVNPIDLSLSDRFQSEDDDMDRVQSTSLRSESESQAVAPMLTSRSHVPGIEPTQTSGSVIPMAPVIIGPNPRHRVMDNTAGTPLTFYPTGPPVPFVTMLPYYNVPSENGSSDASASHFKGTENGDVGQNIHPEGVNQSEDFSTRGVGHHETPDEHRSDILNSDFASHWQNLQFGRFCQGPHHHGPTVYPSPVMVPPGRVPWDGPGRPLANMNLVTQLMNYGPRLVPVPSLQSVPNRPPNVFQHYVEDMPRYRNGTGTYLPNPKVDVRDRPRKENFNHDRSDNHGHRDSNSKSRGSGRNHNRNQTDKSTAKSSGSWSMNSRRHGSFSTYQTQNGILHPGSSQSGPTNVNYGMYPLPGVPPNGSDGPVVELGSPGPAGFSGVNEQSPRGEGSRYRRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CJF4</accession>
    <name>A0AAD5CJF4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CENP-V/GFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_004528</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Gfa family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007776">
        <property type="protein sequence ID" value="KAI7743178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CJF4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016846">
        <property type="term" value="F:carbon-sulfur lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052355">
        <property type="entry name" value="CENP-V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006913">
        <property type="entry name" value="CENP-V/GFA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011057">
        <property type="entry name" value="Mss4-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28620:SF1">
        <property type="entry name" value="CENP-V_GFA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28620">
        <property type="entry name" value="CENTROMERE PROTEIN V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04828">
        <property type="entry name" value="GFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51316">
        <property type="entry name" value="Mss4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51891">
        <property type="entry name" value="CENP_V_GFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CENP-V/GFA" evidence="4">
        <location>
            <begin position="8"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7743178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="135" mass="14987" checksum="D77B98E6446FC7D8" modified="2024-05-29" version="1">MDTELVIHNGGCHCGRVRWRVQAPASVTAWQCNCSDCDMRGNTHVIVPSQRFELLEGSKEFLTTYTFGTHTAKHTFCKVCGITSFYTPKSNPDGIAITYKCVDSGTLTHVEIKHFDGLNWEKSHKSTGIASCSKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CJY6</accession>
    <name>A0AAD5CJY6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ATP synthase epsilon subunit C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_005811</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008041">
        <property type="protein sequence ID" value="KAI7742220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CJY6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001469">
        <property type="entry name" value="ATP_synth_F1_dsu/esu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020547">
        <property type="entry name" value="ATP_synth_F1_esu_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13822">
        <property type="entry name" value="ATP SYNTHASE DELTA/EPSILON CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13822:SF10">
        <property type="entry name" value="ATP SYNTHASE EPSILON CHAIN, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00401">
        <property type="entry name" value="ATP-synt_DE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP synthase epsilon subunit C-terminal" evidence="2">
        <location>
            <begin position="27"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="30"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7742220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="74" mass="8196" checksum="7CD6B11F7F4C46B2" modified="2024-05-29" version="1">MALMGNFARIGNNEIIVLVNDAEKSSDIDLQEAQQTLEIVEAALRKAKGKRKTIEANLAFRRARTRVEAINAIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CKC1</accession>
    <name>A0AAD5CKC1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 14">Pyrroline-5-carboxylate reductase</fullName>
            <ecNumber evidence="4 14">1.5.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">M8C21_007257</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">AA19_3_7</strain>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-proline + NAD(+) = (S)-1-pyrroline-5-carboxylate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:14105"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17388"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="EC" id="1.5.1.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 14">
            <text>L-proline + NADP(+) = (S)-1-pyrroline-5-carboxylate + NADPH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:14109"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17388"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="EC" id="1.5.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Amino-acid biosynthesis; L-proline biosynthesis; L-proline from L-glutamate 5-semialdehyde: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the pyrroline-5-carboxylate reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.2" evidence="4 14"/>
    <dbReference type="EMBL" id="JAMZMK010008074">
        <property type="protein sequence ID" value="KAI7742116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CKC1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004735">
        <property type="term" value="F:pyrroline-5-carboxylate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055129">
        <property type="term" value="P:L-proline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3730.10:FF:000001">
        <property type="entry name" value="Pyrroline-5-carboxylate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000190">
        <property type="entry name" value="Pyrroline-5-carboxylate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3730.10">
        <property type="entry name" value="ProC C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01925">
        <property type="entry name" value="P5C_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028939">
        <property type="entry name" value="P5C_Rdtase_cat_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053790">
        <property type="entry name" value="P5CR-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029036">
        <property type="entry name" value="P5CR_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000304">
        <property type="entry name" value="Pyrroline-COOH_reductase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00112">
        <property type="entry name" value="proC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11645">
        <property type="entry name" value="PYRROLINE-5-CARBOXYLATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11645:SF0">
        <property type="entry name" value="PYRROLINE-5-CARBOXYLATE REDUCTASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03807">
        <property type="entry name" value="F420_oxidored"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14748">
        <property type="entry name" value="P5CR_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000193">
        <property type="entry name" value="Pyrrol-5-carb_rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00521">
        <property type="entry name" value="P5CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 13 14" id="KW-0521">NADP</keyword>
    <keyword evidence="10 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8 14" id="KW-0641">Proline biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyrroline-5-carboxylate reductase catalytic N-terminal" evidence="15">
        <location>
            <begin position="13"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="Pyrroline-5-carboxylate reductase dimerisation" evidence="16">
        <location>
            <begin position="168"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="17"/>
            <end position="22"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>NADPH</name>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00050547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00052690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000193-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF03807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF14748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAI7742116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="275" mass="28580" checksum="6B7495F33DD071DE" modified="2024-05-29" version="1">MSTVQPIPTDTYKLGFIGAGKMAESIAKGVVKSGVLPASRIQTAHKGARRAAFESIGVSVAEHNNQVVENCDVVIFSLKPQVVKDAIMQLKPLLSEKKLLVSVAAGIKLKDLQEWAGHDRFIRVMPNTPSAVGEGASVMSLGAGATQEDGEIIAKLFGAVGKVWKAEEKLFDAVTGLSGSGPAYIFLVIEALADGAVAAGLPRELALGLASQTVLGAASMATQTGKHPGQLKDDVTSPGGTTIAGVHELEKSGFRGILMNAVVAATKRSRELSQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CLC7</accession>
    <name>A0AAD5CLC7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GB1/RHD3-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_030252</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRAFAC class dynamin-like GTPase superfamily. GB1/RHD3 GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007851">
        <property type="protein sequence ID" value="KAI7742970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CLC7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030386">
        <property type="entry name" value="G_GB1_RHD3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015894">
        <property type="entry name" value="Guanylate-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10751">
        <property type="entry name" value="GUANYLATE BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02263">
        <property type="entry name" value="GBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51715">
        <property type="entry name" value="G_GB1_RHD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GB1/RHD3-type G" evidence="4">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7742970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="123" mass="13479" checksum="F4F677F293EA55CC" modified="2024-05-29" version="1" fragment="single">MVTNTKLLGRSSGFQVASTHRPCTKGLWLWSTPLRRTALDGTEYNLLLIDSEGIDAYDQTGTYSTQIFSLAVLLSSMFIYNQMGGIDEAALDRLSLVTEMTKHIRVRASGGKTTASELGQFSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CNC0</accession>
    <name>A0AAD5CNC0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_029659</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VPS35 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007642">
        <property type="protein sequence ID" value="KAI7743885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CNC0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030906">
        <property type="term" value="C:retromer, cargo-selective complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.660">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 35, helical subcomplex Vps35-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005378">
        <property type="entry name" value="Vps35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042491">
        <property type="entry name" value="Vps35_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11099:SF0">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11099">
        <property type="entry name" value="VACUOLAR SORTING PROTEIN 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03635">
        <property type="entry name" value="Vps35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7743885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="122" mass="13714" checksum="916C99D1D901BBD1" modified="2024-05-29" version="1" fragment="single">TIEVLSMVPAPEFALRLHLECAEAANDCDLEPVAYEFFTQAYILYEEEISDSKAKVTKLYLIIGTLQRMHTFGVENRDTLTHKATGYSEKLLKKPDQCRVDGIKDGESCVEMGHLSFLKGPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CNN0</accession>
    <name>A0AAD5CNN0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Serine aminopeptidase S33 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_017330</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the diacylglycerol acyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007305">
        <property type="protein sequence ID" value="KAI7745268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CNN0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004144">
        <property type="term" value="F:diacylglycerol O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019432">
        <property type="term" value="P:triglyceride biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07987">
        <property type="entry name" value="LPLAT_MGAT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007130">
        <property type="entry name" value="DAGAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22753:SF24">
        <property type="entry name" value="ESTERASE_LIPASE_THIOESTERASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22753">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 68"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03982">
        <property type="entry name" value="DAGAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Serine aminopeptidase S33" evidence="4">
        <location>
            <begin position="147"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7745268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="685" mass="76641" checksum="7A1DA553948B21E1" modified="2024-05-29" version="1">MTITLPSICSIPTVKLTPSIFTMTRKVKPLTSVRPSVASTEQPLTSVKNIEADNINLKRRRLETEVERFSVKDYLEQSREVLMTSDGGPPRWFSPLECGSGSRLDNCPLLISLPGVDGTGLSLLLHHQRLGEIFDIWCLHIPTTDRTSFTDLVKLIETTVTSEHEQYPGRPIYLVGESFGGCLALAVAARVPHIDLVLVLANPATSFGKSPLQPLLPILKIVPDHIRPGLPYIMSLMTGVPSSMLASTAKKGLPTQQTITDLSETVASLFSYLSDVGDALTVETILWKLKMLESACAYSNSCLHSVSAQTLILSSGNDQLLPSLQEGERLKKTLPKCQIRTFSDSGHALFLEENFDLVTVIKGAGFYRRAKHTDYASDFVPPSSHEVKDILDSSRWIDVATSPVMLSTLENGKIVRGLDGIPSEGPVLFVGCHNMLGFEVSPMIRRFIIEKNIILRGVAHPMLFRQLKENRMPDLATYDVTRLLGAVPVSPSNLYKLFSLKSHVLLYPGGVREALHRKGEEYTLFWPEHSEFVRMAARFGATIIPFGAVGEDDYAQLVLDYDDQMKIPFMKDYVKKQTDESIKLRNDIKGEVANQDLHMPVILPKVPGRYYYLFGKAIKTEGRREELRDRERAHQVYVEVKGEVEKCLSYCKTKRENDAYRNIISRLMYQATHGLESQVPTFDLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CQG7</accession>
    <name>A0AAD5CQG7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Sucrose-phosphatase</fullName>
            <ecNumber evidence="9">3.1.3.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_000408</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 9">Catalyzes the final step of sucrose synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>sucrose 6(F)-phosphate + H2O = sucrose + phosphate</text>
            <dbReference type="Rhea" id="RHEA:19289"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17992"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57723"/>
            <dbReference type="EC" id="3.1.3.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 9">Glycan biosynthesis; sucrose biosynthesis; sucrose from D-fructose 6-phosphate and UDP-alpha-D-glucose: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 9">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 9">Belongs to the sucrose phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.24" evidence="9"/>
    <dbReference type="EMBL" id="JAMZMK010007014">
        <property type="protein sequence ID" value="KAI7746243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CQG7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050307">
        <property type="term" value="F:sucrose-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005986">
        <property type="term" value="P:sucrose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02605">
        <property type="entry name" value="HAD_SPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1070.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006379">
        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006380">
        <property type="entry name" value="SPP-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013679">
        <property type="entry name" value="SPP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051518">
        <property type="entry name" value="Sucrose_Phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012847">
        <property type="entry name" value="Sucrose_phosphatase_pln/cyn"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01484">
        <property type="entry name" value="HAD-SF-IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01482">
        <property type="entry name" value="SPP-subfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01485">
        <property type="entry name" value="SPP_plant-cyano"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46521:SF15">
        <property type="entry name" value="SUCROSE-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46521">
        <property type="entry name" value="SUCROSE-PHOSPHATASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05116">
        <property type="entry name" value="S6PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08472">
        <property type="entry name" value="S6PP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01141">
        <property type="entry name" value="C2.B.1:_Sucrose_Phosphatase_Li"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01140">
        <property type="entry name" value="C2.B:_Phosphomannomutase_and_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00043">
        <property type="entry name" value="sucrose-phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sucrose phosphatase-like" evidence="10">
        <location>
            <begin position="9"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="Sucrose-phosphatase C-terminal" evidence="11">
        <location>
            <begin position="283"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU368007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF05116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7746243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="424" mass="48301" checksum="A8AE0605BAD346B9" modified="2024-05-29" version="1">MDRLKSSARLMIVSDLDHTMVDHHDPENFSILRFNALWEAEYRHDSLLVFSTGRSPTLYKQLRKEKPMLTPDITIMSVGTEITYGNEMVPDEGWVEVLNHKWDKKIVTEETSKIPELTLQSETEQRPHKASFYVKKEKAQEIMKSLSESLIKRGLDVKIIYSGGMDLDILPQGAGKGQALAYLHTKLKAEGKLPQNTLACGDSGNDAELFTIPDVHGVMVKNAQEELLQWHAENAKNNPKIIHADERCAAGIIQAIGHFNLGPNTSPRDLPDLLDAKLDHFDPAYEVVKFYLFLERWRRAELETPERYLNNLKAVCSPAGLYVNPLGIEQSFDDVIGKLKDCYGDKKGKQYRVWVDQVFPSQIDSYTWLVKFKKLDQTGEEQQCCYTTAILSSKDVEPSQGLTWVHVHQTWMDASTSDDQSWFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CQV9</accession>
    <name>A0AAD5CQV9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_001582</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007343">
        <property type="protein sequence ID" value="KAI7745160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CQV9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35759">
        <property type="entry name" value="BNAA09G03860D PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35759:SF1">
        <property type="entry name" value="OS07G0673000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042009375" evidence="1">
        <location>
            <begin position="26"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7745160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="334" mass="37472" checksum="F1B08BB50A866616" modified="2024-05-29" version="1">MVNPLLSSLPFKFAFILSFSLTASSSSSSTKPFPKKPSATAGDILALLGTPHQASSVDPHLASQLKSCFKFLVPFTPSTELTPPRSLSDLDIKFPRRMLIPSKHRLCDSNSVTRRQNELVWSPPPSVLDIARLAFDSGADPSSIQRALDPAIIYVPDVEGSNEDRCQLTRTPYGRRFINEELNSYIAFLFKLIADRGPEVGLNVSLNRFDFFHGHLFIAADGRVGILFHAKEYPAYDKEVFPYNMGYCQKGSNVTYDESMNLRNILWLAPLPSNSTSDWSAPGVLVVLDAHPGGIIYRDIIPDYVDYARTIYEGRIRFDHFRIPDYAMSWKDEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CUB2</accession>
    <name>A0AAD5CUB2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_018288</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006920">
        <property type="protein sequence ID" value="KAI7746676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CUB2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34541">
        <property type="entry name" value="OS01G0729900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34541:SF2">
        <property type="entry name" value="OS01G0729900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7746676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="475" mass="52138" checksum="28EFFEF3B3DB4A3D" modified="2024-05-29" version="1">MSVERSFEAWEEVQRHGLDLADRLTHGFTDLIHSHITPPSFTWPNPNPPKLFDVEFAERDFRLLIDNSSNSINYGINGVSAIFDIGNKLGQVGADFGASLNGVVQQFVRRLPVPFRHDEEDTVMMRVDDGVQRAADLGISMQVNEDLGTLAKRFKEFGFDETDKAKDESGGDEVAGVNLKAAGFSGRSQGVLNFSTMFDSRTCGLESSLAARGDIWRIEASNGSMTSGNVNSSLFLVQLGPVLFIRDSTLLLPVHLSKKHLLWYGYDRKNGIHSLCPAVWSKHRRWLLMSMLCLNPMACTFMDLQFPNGQLTYVAGEGISTSAFLPLFGGLLQAQGQYPGEMKFSFSCKNKWGTRVTPMVQWPAKSFTLGFEQALAWKRSGLMVRPTVQFSLHPTIGGNNPGLKAELVHSLNEELSLIGGCSLASHPTAFASLSLGRSKWNGNVGKTGVVLRAETPLGNVGRPSFSVQLNSGIEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CUT2</accession>
    <name>A0AAD5CUT2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_024751</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006699">
        <property type="protein sequence ID" value="KAI7747735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CUT2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7747735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="85" mass="9337" checksum="7C431AC4374C2806" modified="2024-05-29" version="1">MPHCVFFALVSPSPSDNTLHELLPKPRWSSTVGRLFKGGFGRFSLGQAKPSLSDQNVLLVLSMTLRYAAMRACLPARVMGSTEQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CWF8</accession>
    <name>A0AAD5CWF8_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_023389</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006580">
        <property type="protein sequence ID" value="KAI7748189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CWF8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007623">
        <property type="term" value="P:circadian rhythm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039928">
        <property type="entry name" value="LNK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33334">
        <property type="entry name" value="PROTEIN LNK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33334:SF8">
        <property type="entry name" value="PROTEIN LNK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="236"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="269"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="374"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="506"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="564"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="237"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="375"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="511"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7748189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="608" mass="67438" checksum="6F8614F638DB14A8" modified="2024-05-29" version="1">MSDLSMYELEDIIWDEFDRGDDHIVPHPTNARSSRNSSEGDSFKKHRRETTPVLSNTRNFDASHTICQEKEEKDYKPLDKKKKMMEKDSPSHASHGVFNGSCDGEIVKDMPNLASDDIRMPDDCFKSSYATSATGDNNSYSYPLTQMSQAGDLCFVDNNGQDKESGDLLYYSWPDIGNFDDVDKMLRSCDSSFGLGVTDNNDELGWFTSAQIEGTGEALKMDFKFTCPEPSGLKNILHPDHKSPESNNHRSSCISESKDEINFKDQINMQKKKSKHHNQTEGKKAGQGVGNVNGSFYQISDLRSNDSGLSSNNKSDQVFTSIGNRQQHGNLELDYFGHMQSDTCYISPDYLHQTAAVPMLSGIRSEQKGLKSLSPIGSSYASNQSSGGPTFEGNAAEVDEKKPSQGFQPSFIGSPRQSMMFQSSKGVLEQDKLSVKEHDQRNTEGDQKGVDIGLLNVQESSSVSSRLDEISLEATSYRQLRQVTEQLDMRTKLCIRDSLYRLARSAEQRHNNPSLSSGATDVNDLGGPLMSERTNNGFMDMETDTNPIDRSIAHLLFHRPSESLKLPTPFPPNPNLNHGSLPGQSMLAEKRVCEEEAAGESNDTMGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CXR3</accession>
    <name>A0AAD5CXR3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="1">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_016951</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="1"/>
    <dbReference type="EMBL" id="JAMZMK010006381">
        <property type="protein sequence ID" value="KAI7749355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CXR3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047129">
        <property type="entry name" value="PPA2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45619">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE PP2A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="18"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="6">
        <location>
            <begin position="1"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7749355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="238" mass="27211" checksum="0E9F97282635E2B7" modified="2024-05-29" version="1" fragment="single">VRVLCEKAKEILIVESNVHVYEITLIVLLVALKVRYPQRETIKVVRLLKFMDFMMSAYGSEYSMVCVLWFPIYSFIVTNRVLESVKYGNASVWKTFTDLFDYYPLTALVESEIFCLHGGLSPSIETLDNIRNFDRVQEVPHEGPMCDLLWSDPDDRCGWGISPRGAGYTFGQDISEQFNHTNGLKLIARAHQLVMEGFNLGHEQKVVTIFSAPNYCYRCGNMASILEVDDCKGHTFIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CY47</accession>
    <name>A0AAD5CY47_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ras-related protein RABB1c</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_005056</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Intracellular vesicle trafficking and protein transport.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006525">
        <property type="protein sequence ID" value="KAI7748460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CY47"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01866">
        <property type="entry name" value="Rab2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000263">
        <property type="entry name" value="Ras-related protein RABB1c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0636">Prenylation</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00060176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7748460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="177" mass="20093" checksum="A45FA17D63AE9BBA" modified="2024-05-29" version="1">MSYAYLFKYIIIGDTGVGKSCLLLQFTDKRFQPVHDLTIGVEFGARMITIDNKPIKLQIWDTAGQESFRSITRSYYRGAAGALLVYDITRRETFNHLASWLEDAQQHANANMTIMLIGNKCDLAHRRAVSTEEGEQFAKEHGLIFMEASAKTAQNVEEVDSCLLYYHTSLLPLYNNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CZA9</accession>
    <name>A0AAD5CZA9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Glycosyltransferase 2-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_031125</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the glycosyltransferase 2 family. Plant cellulose synthase-like C subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005996">
        <property type="protein sequence ID" value="KAI7751068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CZA9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099402">
        <property type="term" value="P:plant organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048868">
        <property type="term" value="P:pollen tube development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000007">
        <property type="entry name" value="probable xyloglucan glycosyltransferase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32044">
        <property type="entry name" value="GLUCOMANNAN 4-BETA-MANNOSYLTRANSFERASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32044:SF44">
        <property type="entry name" value="XYLOGLUCAN GLYCOSYLTRANSFERASE 12-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13632">
        <property type="entry name" value="Glyco_trans_2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="405"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="552"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="577"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="11">
        <location>
            <begin position="239"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00061151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF13632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7751068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="603" mass="69113" checksum="8AA34795397BCF93" modified="2024-05-29" version="1">MTSSFTWWRSTTTHHHGTPVVVKMENPNNWSMVELQSPTEDDFLNDTVSSKGRNKNAKQLTWVLLLKAHRAAGCLTAIAPATFFLASAVRRRLYAESCAYYKGWHFGAPDLDFGYLYSLRNGIKPVVKDGGLPDVESGDNEGFFPMVLVQIPMCNEKEVYQQSIGAVCNLNWPKSKILIQVLDDSDDPATQLLIKDEVHKWKNEGVNIVYRHRVIRDGYKAGNLKSAMNCGYVKDYEFVAIFDADFQPNQDFLIKTVPHFKDNEELGLVQARWSFVNRDENLLTRLQHVNLAFHFEVEQQVNGIFLNFFGFNGTAGVWRIKALEESGGWLERTTVEDMDIAVRAHLHGWKMIFLNDVECQCELPESYEAYRKQQHRWHSGPMQLFRLCLPDIIKSKISIWKKANIIFLFFLLRKLILPFYSFTLFCIILPMTMFVPEATLPAWVVCYIPATMSFLNILPSPKSFPFLVPYLLFENTMSVTKFNAMISGLFQLGSAYEWVVTKKSGRSSEGDLVSLVESKEIQRAGSEPDLEKLEAELKEKEKKKKHKKHNRIYTKELMLAFLLLTASLRSLLSAQGIHFYFLLFQGLSFLLVGFDLIGEQIAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D088</accession>
    <name>A0AAD5D088_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">endo-polygalacturonase</fullName>
            <ecNumber evidence="3">3.2.1.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">M8C21_014765</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">AA19_3_7</strain>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>(1,4-alpha-D-galacturonosyl)n+m + H2O = (1,4-alpha-D-galacturonosyl)n + (1,4-alpha-D-galacturonosyl)m.</text>
            <dbReference type="EC" id="3.2.1.15"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the glycosyl hydrolase 28 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.15" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010005867">
        <property type="protein sequence ID" value="KAI7751621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D088"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004650">
        <property type="term" value="F:polygalacturonase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.10:FF:000032">
        <property type="entry name" value="Pectin lyase-like superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000743">
        <property type="entry name" value="Glyco_hydro_28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31375">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00295">
        <property type="entry name" value="Glyco_hydro_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00710">
        <property type="entry name" value="PbH1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00502">
        <property type="entry name" value="POLYGALACTURONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0134">Cell wall</keyword>
    <keyword evidence="10" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="9 13" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="8 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 14" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041979037" description="endo-polygalacturonase" evidence="14">
        <location>
            <begin position="31"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAI7751621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="449" mass="48740" checksum="43158BC7D9E62535" modified="2024-05-29" version="1">MKNQNMKNICMFGILTCVLVLFLQHQGVDATREMRHHHDSRLLKETFYPRYGETNPSSLPYMKTSTIFNVLAFGAKGDGTTDDTNAFEAAWLAACKLEASTMVVPKGSVFLIKAISFSGLNCESNIVFQLDGNIIAPKRSGSWESGLLQWLEFTKLNGITIRGTGVIDGQGAGWWSESSDTKPTAIRFYGSSDVTVTGITIQNSQQTHLKFDNCQSVQVFEVTISSPGDSPNTDGIHLQNSQDVTIHSTKLACGDDCVSIQTGCSAVNIQNVDCGPGHGISIGSLGKDNTKACVSNITVRDTKIHDTENGVRIKTWQGGYGSVQGVTFSNIQVSKVETPIIIEEYYCDGGKCQNKGSSVSISDISYENIRGTYTKNPVRFACSDGTPCTDVNLDTIQLQPVQQNSDTGKPFCSNAYGELKTTTSPPIDCLMAAKLSKKQSQNNYDSCQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D114</accession>
    <name>A0AAD5D114_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_013374</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006202">
        <property type="protein sequence ID" value="KAI7750126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D114"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003908">
        <property type="term" value="F:methylated-DNA-[protein]-cysteine S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001497">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_AS"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00374">
        <property type="entry name" value="MGMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7750126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="165" mass="18748" checksum="8C063F78D07350FA" modified="2024-05-29" version="1" fragment="single">TACTNADVDIGPLSSRSKQKVQATRQRSVLMLLRLLGAKGSQVRFSTPKTTYRRLSSRGIRLFGFLYTLDRRLEVSTRMLIRLRCVPCHRIMADPTCNTFLGSSPSTNKSWNIEYAAVLDSSPSSNNSWNMMKEILQGGVLISRPKVIYCMLNSRYKYAHNEELH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D1H1</accession>
    <name>A0AAD5D1H1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Senescence domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_008065</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005730">
        <property type="protein sequence ID" value="KAI7752193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D1H1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009686">
        <property type="entry name" value="Senescence/spartin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045036">
        <property type="entry name" value="Spartin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21068">
        <property type="entry name" value="SPARTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21068:SF43">
        <property type="entry name" value="SPARTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06911">
        <property type="entry name" value="Senescence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Senescence" evidence="2">
        <location>
            <begin position="249"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="25"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="59"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7752193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="475" mass="51975" checksum="599E995F12A36559" modified="2024-05-29" version="1" fragment="single">NPNTTPPYHNNINTNLIPSPPPLPSSSNPKNPPSSSSSSPSSSPPHYPTIDQNDFVEYPSPDSPCSPNSPHDAFESSEDTIMSIPGAYLHLIDTYHSVELAIGYFSIMQLRQGNTVIAIFARVGNDIQWPINRDGACVKLDRTHYFFSIRAPKEHENDDDMLNYGLTFALKGQEDALKDLDELLEIYSRFSVHKMEEKDGVLDVTMAESMSPSDLKSDSKKRVMEQSCRAYWTTLAPNVEDYSGTAAKLIAVGSGHLVKGILWCGDVTVDRLKRGNEVLKMKIGPGTDRAVSPKTMKNIRRVKKVTKMTEKVAGGVLSGVLKITGYFSTSVANSKLGKKFFKHVHGETALATLDGFSKVCDAFEVSGRHVMSTSSTVTTELVTHKYGEETAKATNEALHAGGHAIGTAWTVFKIRTAVMNPRSAMAPVLLGKGGIRNATEEMKAKAIKDMKAKAIKDGKAKESPKKHKSRRCICM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D2F9</accession>
    <name>A0AAD5D2F9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Armadillo-like repeats domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_029524</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006098">
        <property type="protein sequence ID" value="KAI7750680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D2F9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009941">
        <property type="term" value="C:chloroplast envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055241">
        <property type="entry name" value="Armadillo_rpt_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36793">
        <property type="entry name" value="RIBOSOMAL RNA SMALL SUBUNIT METHYLTRANSFERASE J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36793:SF1">
        <property type="entry name" value="RIBOSOMAL RNA SMALL SUBUNIT METHYLTRANSFERASE J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22915">
        <property type="entry name" value="ARMH5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="135"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="Armadillo-like repeats" evidence="3">
        <location>
            <begin position="205"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="69"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7750680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="356" mass="39462" checksum="E68EB0E866DD8E77" modified="2024-05-29" version="1">MVMSITSCGHHYLFCNASSSSSSSSTKRHPPSLSQLTITNKSTQLNTIITTTTFNIPSLILLSSSPSPLRPTRLLSSTVPSASTNNNTNPNDSDKKEEVVEEDLPWIQEKALDIVEFSGSVTQTLPGPRVGRSSFPWLLALPLAYVGVSFVIALLNTLRKFTSPKHARRRLVSKNAELCRSIDDLLDKETNGLQQQVLKELMQKTGFSMVEILRKYIRYSLNEKPFDPKLVAALIQLRKATMLDDSQVAEILNEVSRRIVKDFGPVVMNTSGYSEKGLRRKLAVQALFGKILYLSELHEFCARDSSLIVKEIFGVADEDAERLRLHTVAEAGDMDSLEKMVVGVDSEPDSEDSGED</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D2X2</accession>
    <name>A0AAD5D2X2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_008378</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the iron/ascorbate-dependent oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005762">
        <property type="protein sequence ID" value="KAI7752077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D2X2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.330:FF:000001">
        <property type="entry name" value="Protein SRG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050295">
        <property type="entry name" value="Plant_2OG-oxidoreductases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47991">
        <property type="entry name" value="OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0408">Iron</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="6">
        <location>
            <begin position="207"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7752077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="372" mass="42272" checksum="17F4E662A3A619E9" modified="2024-05-29" version="1">MEPKGTSFGGSLLVPSVQELAKEPLLEIPSRYIRTDQDPIVKSSVTSLPTTELPIIDMQLLMSDDSAAHELERLHIACKEWGFFKLINHGLSSALLEKVKKETQEFFNLPMDVKKKFWQHEGDMEGFGQGFVMSEEQKLDWADMFYIVTLPHDMRKPHLFPKLPLPFRDTLETYSSDVKKISLSTLMFIAKASKMKAEDMKVLFEEGLQSMRMNYYPPCPQPEKVIGFGQHSDSFGITFLLQINEVEGLEIKKDGNWTPVKPLPNSFIVNVGDTLEVISLSQPPVMFHYQIWIVTNGLYKSIEHRAMVNSNKERLSIATFLAPNLDGKIGPAPSLITPETPSRFLSVSVMDYIKDLFSGRIHGKSNIEKYRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D4A2</accession>
    <name>A0AAD5D4A2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_016787</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalytic subunit of the peripheral V1 complex of vacuolar ATPase (V-ATPase). V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the V-ATPase G subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005560">
        <property type="protein sequence ID" value="KAI7753032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D4A2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000221">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase, V1 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.2950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005124">
        <property type="entry name" value="V-ATPase_G"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12713:SF11">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12713">
        <property type="entry name" value="VACUOLAR ATP SYNTHASE SUBUNIT G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7753032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="61" mass="6997" checksum="9ABE41D0B36E8784" modified="2024-05-29" version="1">MTLVVRKLLYTCQESSVDSGANVTRLEKETEGKIQHVRIEADRIAHDVVEMLLKHVTSVRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D6Z3</accession>
    <name>A0AAD5D6Z3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Integrator complex subunit 3 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_030962</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010002083">
        <property type="protein sequence ID" value="KAI7755028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D6Z3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045334">
        <property type="entry name" value="INTS3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019333">
        <property type="entry name" value="INTS3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13587">
        <property type="entry name" value="INTEGRATOR COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13587:SF7">
        <property type="entry name" value="INTEGRATOR COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10189">
        <property type="entry name" value="Ints3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrator complex subunit 3 N-terminal" evidence="1">
        <location>
            <begin position="45"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7755028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="452" mass="52375" checksum="83BB22C0C64EEAE2" modified="2024-05-29" version="1">MAFSLIHKSPYEADNPLESSLTEAFRLLEPELRPPFPLTICTEAEYFNLNRAILYGILSEAHYAKVHFKHLHGIVTDGYCFFTSLVCKVVDELYAKVVESVKVQLVYVCLELVDVSAVGVDGLLVALLRQIRGGDFSESNLWLCSRLVDVFTGKWECLVEEQPMVLSSALYVFLRVLSDHCRVCLNPKLNVLKEKEIEFCVRVFRDQFHLCLRIGRDLIRLLQDLVHVPEFQDIWKDLLSRSKDVSQLYRTRTCSRYFLLRITPEMETHLRFLLTQVKLGSQTRYQIWFGRKFLNVSEKETVVIDIVRFICCAHHPSNDVLLSNVTPRWVIVGWLLKCCTKNHVEANAKLALFYDWLFFDEGVDNIMNIEPAILLMVNSVPKYIHMTHNLLEFLFLLVDHYDIDRKDLILKGVSCALDVLKRKGVVQSFDVLTCCDALSPFLKERLVKMLSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D810</accession>
    <name>A0AAD5D810_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_015515</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000605">
        <property type="protein sequence ID" value="KAI7756076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D810"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006566">
        <property type="entry name" value="FBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31639">
        <property type="entry name" value="F-BOX PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31639:SF315">
        <property type="entry name" value="LEUCINE-RICH REPEAT DOMAIN SUPERFAMILY, F-BOX-LIKE DOMAIN SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08387">
        <property type="entry name" value="FBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="165"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="FBD" evidence="2">
        <location>
            <begin position="378"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7756076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="528" mass="61338" checksum="7CB2C010E6C0A800" modified="2024-05-29" version="1" fragment="single">MKAKRDARGGRKRVLSIFKSSLVPNIQVRNFTFFRLGDLAKMNFMMGETLLAPSLELTTSTVDQKNVSFCFHPKFVKNHSIFFIHLRQDDEDDVKNAFTGKGASFHKNLDQKLGLKNSASEATEMFTVTQVSLHFNSVSASVQRKQPEMKAKRLSRAQRLDIITTLPQNIIEIILCLLPIEEAARTSILSREWRFKWTKIPKLVFDNYTVKRSTEKWNLSNWNQRSDLERKRRDKEMRCKLFYAIHQVLLQHQGPIQEFSLSMQVDETCFEIDQIILHLARNHTLKRLTLAHCDYSAQDWGPGEFPPTLINLKYFCIEELCFGPSNGFAFLGVLFKCSPNLEKIRLEIETDDGYDEIESTEIEEEFSLILEKYSNVWLEHLSELEILHYRNEKLELDLLKFILARSPNLKKVKFGTWMFDLRKDLKMLKTLLRPPRASPLKTYLMLKESQTLFLDSVVATIVYLTQVSLHFNSVSASVQQKQPEMKAKRSSRARRLSKARQLDIITTLPQNIIEIILCLLPIEEAART</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D8M4</accession>
    <name>A0AAD5D8M4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="3 8">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_030521</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FKBP-type PPIase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="3 8"/>
    <dbReference type="EMBL" id="JAMZMK010001857">
        <property type="protein sequence ID" value="KAI7755055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D8M4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070370">
        <property type="term" value="P:cellular heat acclimation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000008">
        <property type="entry name" value="Peptidylprolyl isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.50.40:FF:000012">
        <property type="entry name" value="Peptidylprolyl isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.50.40:FF:000017">
        <property type="entry name" value="Peptidylprolyl isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.50.40:FF:000022">
        <property type="entry name" value="Peptidylprolyl isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050754">
        <property type="entry name" value="FKBP4/5/8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46512">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46512:SF11">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="7 8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0697">Rotamase</keyword>
    <keyword evidence="5 9" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="PPIase FKBP-type" evidence="10">
        <location>
            <begin position="61"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase FKBP-type" evidence="10">
        <location>
            <begin position="177"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase FKBP-type" evidence="10">
        <location>
            <begin position="294"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="487"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7755055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="570" mass="63493" checksum="DE8CDBF03C71DEA9" modified="2024-05-29" version="1">MEDDFDFNAAGAMNDEMMDDVAYDDDAPVTLKVGEEKEIGKEGLKKKLLKEGEGWDTPDNGDEVEVHYTGTLLDGTKFDSSRDRGTPFKFTLGQGQVIKGWDQGIKTMKKGENALFTIPAELAYGESGSPPTIPPNATLQFDVQLLSWLSVKDICKDGGIFKKILNKGENWENPKDLDEVLVNYEVRLEDGTVVAKSDGIEFTVEDGHFCPALSKAVKTMKKGEKVLLTVKPQYGFGEKGKQASGDEGAVPANATLQITLELVSWKTVSDVTKDRRVMKKILKEGEGYERPNEGAVVQVKLIGKLQDGTIFTKKGYDDGAELFEFKTDEEQVIEGLDRAVMSMKKGEVATLTVAPEYAFGSTESKQELATIPPNSTVIYELELVSFVKEKESWDMNTQEKIEAAGKKKEEGNSLFKAGKYLKASKRYEKAVKYIEYDTSFGDEEKKQAKALKVSCNLNNAACKLKLKEYKQAEKLCTKVLENDSRNVKALYRRAQAYINLADLDLAELDIKKALEIDPDNRDVKLEYKVLKEKMREYNKKDAKFYGNIFAKLGALDSKKTAAPMAIDSKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D951</accession>
    <name>A0AAD5D951_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF4378 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_005707</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010001684">
        <property type="protein sequence ID" value="KAI7755194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D951"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33623:SF4">
        <property type="entry name" value="DUF4378 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33623">
        <property type="entry name" value="OS04G0572500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="139"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="139"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7755194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="435" mass="49218" checksum="92CC1E46FED5F059" modified="2024-05-29" version="1">MSSCSSNGFKSYPRRQCCTTVRFLMETDLKGNPTRFPSKRLRRSLADSKLTPKQPSMMQKASDVMIKALKRLQFAGVGVSTPAKLLPRSFSRKLLKHGFWKKNDYQNNNEVKRLTTFGDLIKLEEVATLSSVVNTTTSTDITNSISSSSNNNSSSSSSNSSSNSNSNNNNSNNDVTDDNLEVKLDQDVLLEKEKTNGNRVGVTTVTAIVNNYSDANEDAKKWQVNQDEQFSPVSVMDFPSDNDVDEEDEVSSISPCKHLNVKGTKISTHKTRISDRIPQLEPVKLEDRIAQSVLESSTTPFLDFAQEENQIDKKAMALLLLLKSMVPFHHLLKYDAEESLLLDFFKESTHEENVSDYDMLQRAKNWMDGQEQDISIDWQCENNRQTYLRGIEKGVKWSNYDHQVEKVNVGLEVECEIFASLVNDVLTEFSLQEFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D985</accession>
    <name>A0AAD5D985_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PGR5-like protein 1B, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_007499</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000250">
        <property type="protein sequence ID" value="KAI7756818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D985"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016730">
        <property type="term" value="F:oxidoreductase activity, acting on iron-sulfur proteins as donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009773">
        <property type="term" value="P:photosynthetic electron transport in photosystem I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039987">
        <property type="entry name" value="PGRL1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31032:SF2">
        <property type="entry name" value="PGR5-LIKE A PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31032">
        <property type="entry name" value="PGR5-LIKE PROTEIN 1B, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="140"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="187"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7756818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="302" mass="33273" checksum="5442AE70FFF47E88" modified="2024-05-29" version="1">MAAGTSSSNVIGSAVVDLSRTSRTLPPSQLVRFSCKNSTNDGLSIRTDKVRQPSSQVLVEGPSCLFVGPIESASQETLEALYRQARDAYYSGKPLIVDDMFDRVELKLRRYGSKYVVKYPRCSLRQQSTYADAEEDPAQVFALASVWLLFLGIGSSACLVPVIYTFGQAFKDALDSGFSATGSQAPILEFIAILNGMLVMMLGSMIGYPVASASVGALQGLWKSDLVALKGVCPNCGEEVFAFVKSNRFNYSPHKAECHVCECLLEFRTKVERSPASPGKRWVYGRIYLIRRRRMNRGGRWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DB36</accession>
    <name>A0AAD5DB36_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_015496</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000484">
        <property type="protein sequence ID" value="KAI7756262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DB36"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34121:SF1">
        <property type="entry name" value="FILAMIN-A-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34121">
        <property type="entry name" value="MYOSIN-11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="156"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="570"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="656"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="427"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="166"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="662"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="684"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7756262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="754" mass="84989" checksum="AED1D610F62BCB12" modified="2024-05-29" version="1" fragment="single">QLAKILDFHRQSGKESHISRVQTQLSQTSQNQNLPHPINQSTHHHFNKQISASHTRSSSLQMSWLRSAVNKAVEVGNKNNLTHIVKNYADTVVQHAGQAVAGGAKMFHDRLGARSFKSFKQTVKRLEEASISARGAERILLMRRWLAALKETEKLSGGSVEDDEKNQEPHPPSEELSDNPKKPSLRSWQVLYYDSEMVGDPMNFRDVFLYSQALEGITISMILEPPSEEEVSLLLEFFGQIILVFYSCCLTGGKEVHNAIVSSIQDLAKAFSGYEEEVLVKREELLQFAQGAITGLKVNAELKRIDAEASVLKEKLDGMESSGEIIGDGYEKASKEATIATIEALKKALAHIRVCSKLEGLLLKKKRLYGGETPEIHAQKVDKLKVLSESLVSSATKAEKRISDNRVQKEEALNFRVSKAGEVGELEKELAAEVAGLEKQRNDLEAELKRVNISLSAANGRLQNVREERDQFYEANDQLVAHLKTKEDELVKSISSCKQEVNVLNTWVNFLEDTWVLQRANTEIKEKQVSDELAKHESYFVNLVAKLLSAYEEGLKPSVDRIEKYVENLKSLSGGSDPPSNVEDKEASKVINPRRSLEEEYLDYEAKIITTFSVVDNMKEQFYSQHGKASRKEDTRIKELFENIEKLRVKFDSIERPNLEMENPDEVEETADAETSTKPADKPQVQVQTQAQTQTETRTSEQHVKSPTAKGDQGLDPEAEIAKLESEFGKVNREYTEEEIGDWEFDELEKELSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DCI9</accession>
    <name>A0AAD5DCI9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Wall-associated receptor kinase galacturonan-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_031432</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000138">
        <property type="protein sequence ID" value="KAI7757304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DCI9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46008">
        <property type="entry name" value="LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-LIKE 1.4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46008:SF25">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042185706" description="Wall-associated receptor kinase galacturonan-binding domain-containing protein" evidence="4">
        <location>
            <begin position="20"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="244"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7757304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="267" mass="30286" checksum="727C8E316AEA050E" modified="2024-05-29" version="1" fragment="single">MIIVFCFISLFLLCHSAAADTNLSMPISCPERFECPGFVPFGYPFYDVTDTRCGLIKVNCTSKEIQFGGRSYEFIGKFDLSPNSDSIVIYNRTFEQLVRNKSSACDALMYNFTSPSPSPLLYSVSIFPTITLFKCPKNLTYFEKHKYNSYNSCKDHTFYYNYFNGTIPTYLPITCQVVQLPQKISTMPGIDETNIFSLISPVAGIVFKLSDSCKECRKKGVQTGGEQTPITNRGHRKTRHRLDIMLVIAGAFTALILSFVIFIIWRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DCU3</accession>
    <name>A0AAD5DCU3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Autophagy-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_004042</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the ATG8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000194">
        <property type="protein sequence ID" value="KAI7757020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DCU3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004241">
        <property type="entry name" value="Atg8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10969">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEINS 1A/1B LIGHT CHAIN 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02991">
        <property type="entry name" value="ATG8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0072">Autophagy</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="24"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="99"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="24"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7757020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="274" mass="29848" checksum="4084EA59635B7145" modified="2024-05-29" version="1">MEDEATVASLTIIGGMPDVSTMLCTRNESPYSHSSRSQLGDPNAAGGSGKVGEKLVGGTEGGETVGKVFAGTGLLKPSGSSTHDPKHIYEGLTEDSSLDLNVKARKRKSKPTKEEKKNPKKVKILKSNASVSASVSPHGHIMENLDMHLIGGQSSREVVVMLLKCLKFSESSNNNKSAGIGMVFKLQHPPEKRRAESTCIRHKYPDRLPVIVERAERSHIADIDKNKYLVPADLTVGQFVYVVRKRIKLSPEKAIFVFANNMFTTNCCLDVCNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DDX2</accession>
    <name>A0AAD5DDX2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF4228 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_001518</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000210">
        <property type="protein sequence ID" value="KAI7756966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DDX2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025322">
        <property type="entry name" value="PADRE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33413:SF4">
        <property type="entry name" value="D-RIBOSE-BINDING PERIPLASMIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33413">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14009">
        <property type="entry name" value="PADRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="104"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="132"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7756966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="159" mass="17916" checksum="EC8A33A81787A120" modified="2024-05-29" version="1">MGNCQAVDAAALVIQHPNGRIQRMYWSITASQVMKMNPGHYVSLIIPLPEDDASQTVRFTRVKLLRPTDTLVLGRAYRLITTNEVMKVVRAKKHAKMKNKPPEDNQSCSNETVEDNTNNQVEGHERMFRQRSGSSSSILAASRSKSWRPSLQSIYEATT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DE35</accession>
    <name>A0AAD5DE35_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Sulfhydryl oxidase</fullName>
            <ecNumber evidence="11">1.8.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_010579</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>2 R'C(R)SH + O2 = R'C(R)S-S(R)CR' + H2O2</text>
            <dbReference type="Rhea" id="RHEA:17357"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:16520"/>
            <dbReference type="ChEBI" id="CHEBI:17412"/>
            <dbReference type="EC" id="1.8.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.8.3.2" evidence="11"/>
    <dbReference type="EMBL" id="JAMZMK010000026">
        <property type="protein sequence ID" value="KAI7758132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DE35"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016971">
        <property type="term" value="F:flavin-dependent sulfhydryl oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003756">
        <property type="term" value="F:protein disulfide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.310:FF:000004">
        <property type="entry name" value="Sulfhydryl oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000244">
        <property type="entry name" value="Sulfhydryl oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.310">
        <property type="entry name" value="ERV/ALR sulfhydryl oxidase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036774">
        <property type="entry name" value="ERV/ALR_sulphydryl_oxid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017905">
        <property type="entry name" value="ERV/ALR_sulphydryl_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039798">
        <property type="entry name" value="Sulfhydryl_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22897">
        <property type="entry name" value="QUIESCIN Q6-RELATED SULFHYDRYL OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22897:SF24">
        <property type="entry name" value="SULFHYDRYL OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04777">
        <property type="entry name" value="Evr1_Alr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69000">
        <property type="entry name" value="FAD-dependent thiol oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51324">
        <property type="entry name" value="ERV_ALR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 11" id="KW-0274">FAD</keyword>
    <keyword evidence="4 11" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="405"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="ERV/ALR sulfhydryl oxidase" evidence="12">
        <location>
            <begin position="232"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU371123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7758132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="448" mass="51172" checksum="03FF7EFD4F359EFC" modified="2024-05-29" version="1" fragment="single">CPACRNYKPQYEKVARLFNGANAVHPGIILMTRVDCANKINTKLCDKFSVTHYPALYWGPPSKFVGGGWNGKNEKGDIRPISVDDARTADRLLKWINTQLGSSYSLDDDKYENDHLPHSNVSDPGQIARAIYDVEEATSFAFDVIVEHKMIKPNTRATLIKFMQLMVAHHPSRRCRRGTADILVNFDDLYPSSKWLADKHEPEDSTGHGPLGKFDICGKEVPRGYWMFCRGSRNDTRGFSCGLWVLLHSLSVRVEDGESQIAFTTTCDFIHQFFICEECRQHFYDMCSRVSTPFSNARDFVLWLWTSHNEVNKRLKKTEESLKTADPKFPKTIWPSKRLCPSCYNDPSQNEESSSIDWDSDEVYKFITSYYGNKLVSLYKEKDKRNIQMKRSAVSEEVLQSTNALVVPAGAALAIAVASCLFGALACFWRSRQKNRKYFHKPQLLKIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DE76</accession>
    <name>A0AAD5DE76_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_027946</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000061">
        <property type="protein sequence ID" value="KAI7757777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DE76"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052462">
        <property type="entry name" value="SLIRP/GR-RBP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48027">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN 87F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="172"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="234"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7757777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="302" mass="32380" checksum="5E7CB631DAB712FE" modified="2024-05-29" version="1" fragment="single">TVARVIRPARTAPELTKRRTGSFVEMMVSVKTESDGVFSECFGMIYGRFGLLIVAVEVEAGCHVEARRPFECYSSSPSMSKQVVDACRLLRPKATTDLAFGVSSSAGLSDSVDDDGGGEVIRVTEEARTPATGCAVVRDPNCNDPERMSFLVPGTDYSRNDSTILQWPCLDFVGGLDPSVSDEALRQVFGQFGEIVHVKIPAGKRCGFVQFADRASFINHSILNGTKLGGQSVRLSWGRSPSSKQGQPDQAQYDGGGGGGPYYGYAQGYEAYGYAPPPQDPNAYYGVYAGAGGYGGYQQPQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DFA9</accession>
    <name>A0AAD5DFA9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DEK-C domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_013693</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000005">
        <property type="protein sequence ID" value="KAI7758332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DFA9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000779">
        <property type="term" value="P:regulation of double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000220">
        <property type="entry name" value="DEK domain-containing chromatin associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044198">
        <property type="entry name" value="DEK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014876">
        <property type="entry name" value="DEK_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13468:SF22">
        <property type="entry name" value="DEK DOMAIN-CONTAINING CHROMATIN-ASSOCIATED PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13468">
        <property type="entry name" value="DEK PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08766">
        <property type="entry name" value="DEK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109715">
        <property type="entry name" value="DEK C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51998">
        <property type="entry name" value="DEK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="DEK-C" evidence="8">
        <location>
            <begin position="534"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="36"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="326"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="592"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="46"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="62"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="90"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="163"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="347"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="369"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="381"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="406"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="429"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="441"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="455"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="473"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="498"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="594"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="610"/>
            <end position="619"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7758332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="619" mass="69126" checksum="8B58FE79B7DB28AE" modified="2024-05-29" version="1">MYAGDFLYGKDGDFMYAGDFSEMVMGKKRASSVVAETVENGAVADKAGDDVSEKNEEKQEGVEEMEEEVEVDKEVPEVNKNAQDEEAEVKDDKEGEEEKGNEEPKSESMEVDDKKVPDEETENKDMEVDKSKEEEENTKEEKESKKGAKKKGTEGKSNSKTKASVEKKVKEKKEKEPTTPVAPTIERPVRQRKSVERLVAVIENDSAKEFHILKGSGTALKDIPNVAYKLSKRKVNDDTLKLLHTILFGRRGKALQVKSNILQFSGFVWPDNEEKHKLKVKEKLDRLNKEKLSEFCDIFDMTVSRANKEDIVVKLIDFMSSPHATTSELLAEKDQSTKGKKRKSASKKSTSTSEATPSKSSSKKQKTKSISEEAKKKVAETDDSEEESDEEEKDNEEHEDVNGAPEKSEDVASEEERVSEPEPEEESSKKRKRGSTKSKASKNESSVKPKEKKVVTTPKKTSSSPQKKKLVESLTSGSKVADQSDSGKKTSSRKKKKVDAVEEKPVTPKKSTPKEKSGKKTVKEKGKPESEKQKISDNDLKTAICEILKEVDFNTATFTDILKLLAKRFNTDLTSKKASIKFMIQEELTKLADEAEAEEEDKEEPEKTGKQASGQGVKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G1Q2</accession>
    <name>A0AAD5G1Q2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_006956</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012149">
        <property type="protein sequence ID" value="KAI7724842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G1Q2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd11660">
        <property type="entry name" value="SANT_TRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47122:SF13">
        <property type="entry name" value="HOMEODOMAIN-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47122">
        <property type="entry name" value="MYB-LIKE DNA-BINDING DOMAIN CONTAINING PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH myb-type" evidence="5">
        <location>
            <begin position="368"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="4">
        <location>
            <begin position="376"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="195"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="242"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="467"/>
            <end position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7724842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="497" mass="54843" checksum="B5A2F8779B9BDE9B" modified="2024-05-29" version="1">MNNNNNNQAEEESLYHSLEEEAIDVDCLLAEPRSDQVSVSGVWCYEEDNLGKCIKTESASYNLGGQGSNINMEGVDATNLNALDVLPPEVGAENFLTSNVSPCDDYLLDFGFDHGACIGYASNERLHVGNSFEIQNSLSENITGTECQNYFLSNCISGTSTNSSEPKSLDRFVEFSKSFIKQDILPPHFSHNGLDITPDCKDGSTSTGSAQDHDGVGAVTQKRSRKPTKRYIDESSILSMKNSKKRREASGCKGKLPGVRRVKSQSEAKPKNKMASSHFDKAIQVPFISQAPTKCQKSDLPAPTECQKSDLPAKPAKASNQFISQAPTKCQKSNSLVKPAKIESRIEVYSSGSEDDSEAIMTNMTSGNQRKLHRLWSVSEVKKLIDGVAHFGVGKWTHIKKLLFSSSVHRTPVDLKDKWRNLLKASHALKGSRIEDDHKRSQPWRPLPKSILCRVRELASVYPYPRENSSKISKSKVPLARHVSSPARIKSNKVPVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G4Z8</accession>
    <name>A0AAD5G4Z8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">eRF1/Pelota-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_003439</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Directs the termination of nascent peptide synthesis (translation) in response to the termination codons UAA, UAG and UGA. Modulates plant growth and development.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic release factor 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011339">
        <property type="protein sequence ID" value="KAI7727648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G4Z8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003747">
        <property type="term" value="F:translation release factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.60:FF:000001">
        <property type="entry name" value="Eukaryotic peptide chain release factor subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000006">
        <property type="entry name" value="Peptide chain release factor subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.960.10">
        <property type="entry name" value="eRF1 domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.60">
        <property type="entry name" value="eRF1 domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042226">
        <property type="entry name" value="eFR1_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024049">
        <property type="entry name" value="eRF1_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005141">
        <property type="entry name" value="eRF1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005142">
        <property type="entry name" value="eRF1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005140">
        <property type="entry name" value="eRF1_Pelota-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004403">
        <property type="entry name" value="Peptide_chain-rel_eRF1/aRF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03676">
        <property type="entry name" value="aRF1_eRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10113">
        <property type="entry name" value="PEPTIDE CHAIN RELEASE FACTOR SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03463">
        <property type="entry name" value="eRF1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03464">
        <property type="entry name" value="eRF1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03465">
        <property type="entry name" value="eRF1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01194">
        <property type="entry name" value="eRF1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55481">
        <property type="entry name" value="N-terminal domain of eukaryotic peptide chain release factor subunit 1, ERF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0341">Growth regulation</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="eRF1/Pelota-like N-terminal" evidence="7">
        <location>
            <begin position="6"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00045523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7727648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="489" mass="55239" checksum="FADC23B8BF90C6BC" modified="2024-05-29" version="1" fragment="single">NLSQCQRETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASNIKTQQRLKLYNKVPPNGLVLYTGTIVTDDGKEKKVTIDFEPFKPINASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFTVDLPKKHGRGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVSGLILAGSADFKTELSQSDMFDPRLQAKILNVVDVSYGGENGFNQAIELSSEILANVKFIQEKRLIGKYFEEISQDTGKYVFGVDDTLKALEMGAVEILIVWENLDYNRYVLKNSVTGEIIIKHLNKDQESDQSNFRDSETNAELEVQDKTSLLEWFANEYKKFGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDIRSFDELSDDVGINSMDDKSSYTPYPHLKRLHCILRHVLNASNNIRGFKLPYQMTNIQGIVLIYELIGLVTLCQISSQARLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G5I6</accession>
    <name>A0AAD5G5I6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">Laccase</fullName>
            <ecNumber evidence="4 13">1.10.3.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Benzenediol:oxygen oxidoreductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Diphenol oxidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Urishiol oxidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">M8C21_004978</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">AA19_3_7</strain>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Lignin degradation and detoxification of lignin-derived products.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>4 hydroquinone + O2 = 4 benzosemiquinone + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:11276"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:17594"/>
            <dbReference type="ChEBI" id="CHEBI:17977"/>
            <dbReference type="EC" id="1.10.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
        <text evidence="13">Binds 4 Cu cations per monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Secreted</location>
            <location evidence="2 13">Extracellular space</location>
            <location evidence="2 13">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the multicopper oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.10.3.2" evidence="4 13"/>
    <dbReference type="EMBL" id="JAMZMK010011240">
        <property type="protein sequence ID" value="KAI7728291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G5I6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052716">
        <property type="term" value="F:hydroquinone:oxygen oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046274">
        <property type="term" value="P:lignin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13849">
        <property type="entry name" value="CuRO_1_LCC_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13875">
        <property type="entry name" value="CuRO_2_LCC_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13897">
        <property type="entry name" value="CuRO_3_LCC_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000049">
        <property type="entry name" value="Laccase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000062">
        <property type="entry name" value="Laccase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001117">
        <property type="entry name" value="Cu-oxidase_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033138">
        <property type="entry name" value="Cu_oxidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002355">
        <property type="entry name" value="Cu_oxidase_Cu_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034288">
        <property type="entry name" value="CuRO_1_LCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034285">
        <property type="entry name" value="CuRO_2_LCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034289">
        <property type="entry name" value="CuRO_3_LCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017761">
        <property type="entry name" value="Laccase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03389">
        <property type="entry name" value="laccase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF400">
        <property type="entry name" value="LACCASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00394">
        <property type="entry name" value="Cu-oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00079">
        <property type="entry name" value="MULTICOPPER_OXIDASE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00080">
        <property type="entry name" value="MULTICOPPER_OXIDASE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13" id="KW-0052">Apoplast</keyword>
    <keyword evidence="10 13" id="KW-0186">Copper</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 13" id="KW-0439">Lignin degradation</keyword>
    <keyword evidence="7 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 13" id="KW-0964">Secreted</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041784053" description="Laccase" evidence="13">
        <location>
            <begin position="28"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="16">
        <location>
            <begin position="42"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="14">
        <location>
            <begin position="168"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="15">
        <location>
            <begin position="437"/>
            <end position="564"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAI7728291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="582" mass="64587" checksum="0561D6A443202A1D" modified="2024-05-29" version="1">MGVPSSFLATFLFLSCFICFYPDLAIAKHRVTGITRHYKFNIRMHNVTRLCQTKSIITVNEKFPGPRIIAREGDRLVIKVVNHVPNNITIHWHGIRQLRSGWADGPAYITQCPIQTGQSYIYNFTITGQRGTLWYHAHVSWIRATLYGPIVILPRRNTSYPFVKPYKEVPIIFGEWWKADTEAVINQALQTGAGPNNSDAYTINGLPGPLYNCSSPKETFRLKVKPGKTYLLRLINAALNDELFFKITNHTFTVVDADASYVKPFETDTIFITPGQTSNVLLKTKNITSNTRFIMAARPYSTAASGTFDNTTVAGVLEYNSNNMPLSNTSIKGIALPPLPAINATSYVANWTNKFRSLGNSQFPVNVPQTVENNYFFTVGLGSDPCPKNQTCQGPNGTKFAASVNNVSFTLPTTALLQARYFGKSNRVFSTNFPSSPLNKFNYTGTPPNNTMVSHGTKVVVLPYNTTVELVMQGTSILGAENHPLHLHGFNFYVVGQGTGNFNSSTDPANYNLVDPVERNTVGVPSGGWVAVRFRADNPGVWFMHCHIEIHLSWGLRLAWVVLDGKLPNQKLLPPPSDLPKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G6C1</accession>
    <name>A0AAD5G6C1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">asparagine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_023061</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.22" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010010821">
        <property type="protein sequence ID" value="KAI7730224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G6C1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004816">
        <property type="term" value="F:asparagine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006421">
        <property type="term" value="P:asparaginyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00776">
        <property type="entry name" value="AsxRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04318">
        <property type="entry name" value="EcAsnRS_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00534">
        <property type="entry name" value="Asn_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004522">
        <property type="entry name" value="Asn-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002312">
        <property type="entry name" value="Asp/Asn-tRNA-synth_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00457">
        <property type="entry name" value="asnS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594:SF34">
        <property type="entry name" value="ASPARAGINE--TRNA LIGASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594">
        <property type="entry name" value="ASPARTYL/LYSYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01042">
        <property type="entry name" value="TRNASYNTHASP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="8">
        <location>
            <begin position="240"/>
            <end position="536"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7730224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="546" mass="61207" checksum="FC8F084D06215FF7" modified="2024-05-29" version="1">MATAALSPATASFRLKPFSTTVRLLSFYNHQLKPNKLKHQTIIITPFFHLPKPLISFNHSNGYITNPRYYSSIVSATVSSGEVTEKSKTESIEGLVGEFRKRLRIADIKGGESEGLDKLGQTLTVRGWVRTLRVQSSVTFIEVNDGSCISNMQCVIDSDAEGYDQVESGLITTGASVCVQGVVVPSQGKKQKIELKISKLVTVGKSDPSFPIQKKKVTREFLRSKAHLRPRTNTFGAVARVRNALAYATHKFFQENGFVWVASPIITASDCEGAGEQFCVTTLIPSTKEAIDSPVDAIPRTKNGLIDWSQDFFEKPAFLTVSGQLNGETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLNDDMACATAYLQYVVKHVLENCKEDMDFFNNWIEKGIIDRLSDVVEKSFVRMTYTDAVELLLKSKKKFEFPDIKAFYMRLNDDGKTVAAMDMLVPRVGELIGGSQREERLDYLETRLDELKLNKESFWWYLDLRRYGTVPHAGFGLGFERLVQFATGIDNIRDTIPFPRAPGSAEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G6K5</accession>
    <name>A0AAD5G6K5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_019895</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010806">
        <property type="protein sequence ID" value="KAI7730302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G6K5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7730302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="37" mass="4134" checksum="4474EFEFDCEDFABD" modified="2024-05-29" version="1">MNQHLGDTLDSAKYHQHHARYTSNLAGLTVVCCGEFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G8C1</accession>
    <name>A0AAD5G8C1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">3-oxo-5-alpha-steroid 4-dehydrogenase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_031249</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the steroid 5-alpha reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010576">
        <property type="protein sequence ID" value="KAI7731008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G8C1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016627">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01801">
        <property type="entry name" value="Ubl_TECR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000269">
        <property type="entry name" value="Very-long-chain enoyl-CoA reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001104">
        <property type="entry name" value="3-oxo-5_a-steroid_4-DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039357">
        <property type="entry name" value="SRD5A/TECR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10556">
        <property type="entry name" value="3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10556:SF28">
        <property type="entry name" value="VERY-LONG-CHAIN ENOYL-COA REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02544">
        <property type="entry name" value="Steroid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50244">
        <property type="entry name" value="S5A_REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="100"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="125"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="164"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="198"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="229"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="262"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="3-oxo-5-alpha-steroid 4-dehydrogenase C-terminal" evidence="11">
        <location>
            <begin position="159"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7731008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="310" mass="36218" checksum="728BCBCF31A4E2AF" modified="2024-05-29" version="1">MKVTVTTRSGRELINGGLDIDTSATVADLEDAIHQRIKKYYPSRQRLTLPHHPQSKEKPIVLQYKKVLKDYTNGNSDTITIVFKDLGPQVSYRTLFFFEYLGPLLLYPVFYCFPVYQYVGYQTKHVIYPVQTYALYYWCLHYFKRIMETFFVHRFSHATSPVSNVFRNCAYYWTFACFIAYYVNHPLYTPVSDFQMKIGFGFGLIMQVANLYCHIILRNLRGRNGNGGYQIPSGFLFNFVTSANYTTEIYQWLGFNVATQTVAGYLFLVVAASIMTNWALAKHRRLKKLFDGKEGRPKYPQRWVILPPFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GB15</accession>
    <name>A0AAD5GB15_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Apyrase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_033033</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the GDA1/CD39 NTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009488">
        <property type="protein sequence ID" value="KAI7735432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GB15"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017110">
        <property type="term" value="F:nucleoside diphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009134">
        <property type="term" value="P:nucleoside diphosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd24041">
        <property type="entry name" value="ASKHA_NBD_AtAPY1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.150:FF:000008">
        <property type="entry name" value="Apyrase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.150">
        <property type="entry name" value="Exopolyphosphatase. Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000407">
        <property type="entry name" value="GDA1_CD39_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11782">
        <property type="entry name" value="ADENOSINE/GUANOSINE DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11782:SF83">
        <property type="entry name" value="GUANOSINE-DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01150">
        <property type="entry name" value="GDA1_CD39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01238">
        <property type="entry name" value="GDA1_CD39_NTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="199"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="229"/>
            <end position="233"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR600407-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR600407-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7735432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="477" mass="52232" checksum="15C3421E1662064E" modified="2024-05-29" version="1">MTKRRQSLSYNESVSDKINRHRGAILVISIPLVLISFVLFVMPGSSRDQSDGVLSGNRRFSPSYSLQSDPTSYRYAVVFDAGSSGSRVHVFCFDKNLDLVHIGNELELFEQLKPGLSAHAADPKEAADSLLPLLEKAEKVVPQSMREKTPVKVGATAGLRQLGVDASERILQAVKDVLKVKSSLKSNDDWVTVLDGTQEGAYQWVTINYLLKKLGKKYSQTVGVVDLGGGSVQMAYAISESDAAKAPRITNGEDTYVKEMFLMGTKYYLYVHSYLKYGLLAARAEILGVAKDSDNPCILAGYNGVYKYGGNEYKVSALPSGSDWNKCREEALKALKVNEPVCTHMKCTFGGVWNGGGGDGQKNLFVASFFFDRAAEAGFVDASKPVAKVTPSDFQKAAQRACQTTLEDAKSHYPKVEPSNLPYMCMDLVYQYTLLVDGFALQPRQQITLVKQVEYQNSLVEAAWPLGSAIEAVSAAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GBY5</accession>
    <name>A0AAD5GBY5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MAR-binding filament-like protein 1-1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_016095</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009276">
        <property type="protein sequence ID" value="KAI7736422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GBY5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000793">
        <property type="term" value="C:condensed chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000796">
        <property type="term" value="C:condensin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007076">
        <property type="term" value="P:mitotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43941:SF5">
        <property type="entry name" value="ELKS_RAB6-INTERACTING_CAST FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43941">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="691"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="162"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="317"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7736422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="748" mass="84171" checksum="E930E398B51634A4" modified="2024-05-29" version="1">MKMMIMMMGFAVGTSPYHHHHSPLFPSITSSSSSQPLFFYSCKRKPHASFNKTTKIYASSIQSNEPNDVVFRHRRAILFVGLSSLPFFNSKAQAFQNLAPPDEPENSARAQALDQIGEVPNVDAPSNPFLTLSSGLGIIAAGVLGALYALAQKEKSANIAAIESMKAELAEKEAAIASMQRSYDSKLQELKEEKNKQIKTSNQEKQSLVSELSSANNTVKGLGQELRKEKQIIQELENQIDRLKVDLKKASDDKQELKVQLKDKLFSIDVLQGRIKLLESETKDKEDNLANVLSALAVKESEFNKLSSMHKETLGELTESMSTIEQLKEEIFKLEKDLELKSSNVDDLNAEIRSLCNEKDVIFKELESYRSQYNELKISSENKADSDAKLLEESGKKLEELKEKLESTLTESAKKGELVNELTDERDGLRKTLDDELTKVMNLENELQVVQEILEDMRNEAAELDGLLEHSRDTCENLKAELSRVQTEFSESRTALQENIDSLKQTAEMLTNELGSTKDAYAKTMEELNRMSVEIQEIAESRDSLSKELVETYKKLENAINELKEEKENVGSLTKEVNSLKSKILEDKESRKSMENDLEEATKALDEMNQNAKILTRELEISKTKISGLEDEKEVLYKSLDEQKRAVQEARENIEDAHSMIMKLGKEREMLDKKGSKLEKELAAAKGEILRLRNEASSSKPAKKVEKDGVEENAEEVSSNESGSEDEKAPAVKKVGRRKKVVSQQKES</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GCM9</accession>
    <name>A0AAD5GCM9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FYVE zinc finger domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_007187</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009053">
        <property type="protein sequence ID" value="KAI7737367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GCM9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000813">
        <property type="term" value="C:ESCRT I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070676">
        <property type="term" value="P:intralumenal vesicle formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036258">
        <property type="term" value="P:multivesicular body assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045893">
        <property type="entry name" value="FREE1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46977">
        <property type="entry name" value="PROTEIN FREE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46977:SF1">
        <property type="entry name" value="PROTEIN FREE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FYVE zinc finger" evidence="5">
        <location>
            <begin position="307"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="14"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7737367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="454" mass="49084" checksum="12A3A9387BDF5BBB" modified="2024-05-29" version="1" fragment="single">YKYNGFVQTPVVKSISDSASDSVSTESVAESTTESISESWTESGTESGTESRTESVAKSGSGYGGNNYGRVGSDIGVGGVDYYGKRADTGVSRYESGGGGGGYGDGVYAYQGGKVEPYGARGTGSKSSTWKEQVMFDDFGRPIGLPGKETVSAPKVVKAVPKAETREDVKGGVQKFRVKLLAESGGQSTQDVLCQIGLDGIRMLDPSTSRTLRIYPLDTVTRCEVYDSSTFAFWSKSSVDIEPRRIRLQSNRYTTNTLLDTVTAATIQFKEIGGSSRPVEAAKFTEQPTEKKKGLGDWMNIIKPPNEEKDHWVPDEAVTKCTGCGSDFSAFNRKGRTALTSEENAPQVRVCDRCMLEVSHRLNSAKEAAASRSSGLQSHEDLAKKLQEELERNHRSSSGSKFEVSSRRMKEVACPTCTVHLQVQVPSSGSETIECGTRMRSISALGVCLKLKFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GDD6</accession>
    <name>A0AAD5GDD6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">ADP/ATP translocase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">ADP,ATP carrier protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_023695</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the exchange of ADP and ATP across the membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>ADP(in) + ATP(out) = ADP(out) + ATP(in)</text>
            <dbReference type="Rhea" id="RHEA:34999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:35000"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009381">
        <property type="protein sequence ID" value="KAI7736078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GDD6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005471">
        <property type="term" value="F:ATP:ADP antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140021">
        <property type="term" value="P:mitochondrial ADP transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990544">
        <property type="term" value="P:mitochondrial ATP transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000071">
        <property type="entry name" value="probable ADP,ATP carrier protein At5g56450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002113">
        <property type="entry name" value="ADT_euk_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45635">
        <property type="entry name" value="ADP,ATP CARRIER PROTEIN 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45635:SF31">
        <property type="entry name" value="ADP_ATP TRANSLOCASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00927">
        <property type="entry name" value="ADPTRNSLCASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="14"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="121"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="223"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU368008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7736078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="312" mass="34726" checksum="4BC71DF4F4D9DF03" modified="2024-05-29" version="1">MKMETVGMHTNCLTKFPRDLLAGAMMGGMVHTVVAPIERTKLLLQTQESNIVIVGGKHRRFNGMIDCIVRTVKEEGVLSLWRGNGSSVLRYYPSVALNFSLKDLYRSLLRSGNSQQSTFISGPSANFIAGAAAGCTTLVLIYPLDIAHTRLAADLGKTDSRQFQGIRHFLTTIWAKNGIRGVYKGLPASLQGMVVHRGLYFGGFDTLKEKMSKNAPSDVPLWKRWIVAQGVTTSAGLISYPMDTVRRRMMMQSGIERPMYKNTLDCWKTVYKTEGLRSFYRGALSNMFRSTGAAAILVLYDEVKNFMNWNGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GG82</accession>
    <name>A0AAD5GG82_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Germin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_016171</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Secreted</location>
            <location evidence="1 10">Extracellular space</location>
            <location evidence="1 10">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the germin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008660">
        <property type="protein sequence ID" value="KAI7739196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GG82"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02241">
        <property type="entry name" value="cupin_OxOx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000005">
        <property type="entry name" value="Germin-like protein subfamily 1 member 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006045">
        <property type="entry name" value="Cupin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001929">
        <property type="entry name" value="Germin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019780">
        <property type="entry name" value="Germin_Mn-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31238">
        <property type="entry name" value="GERMIN-LIKE PROTEIN SUBFAMILY 3 MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00190">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00325">
        <property type="entry name" value="GERMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00835">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00725">
        <property type="entry name" value="GERMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0052">Apoplast</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 8 10" id="KW-0464">Manganese</keyword>
    <keyword evidence="5 8 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Cupin type-1" evidence="11">
        <location>
            <begin position="10"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU366015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7739196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="152" mass="16128" checksum="0E91D52CEB845D18" modified="2024-05-29" version="1">MGNTSNDMGSNVTAVAVAELPGLNTLGISLARIDFAPRGINPPHTHPRATEILIVIEGRLLAGFVTSNPENRLITKVLVKGDVFVFPQGLIHFQKNLGKGHAIAIAGLSSQNPGVITIANAMFGSNPDIVGDVLEKAFQVDIKVVHQIQSKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GGZ1</accession>
    <name>A0AAD5GGZ1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_009545</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the aromatic acid exporter (TC 2.A.85) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008614">
        <property type="protein sequence ID" value="KAI7739498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GGZ1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015743">
        <property type="term" value="P:malate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034220">
        <property type="term" value="P:monoatomic ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020966">
        <property type="entry name" value="ALMT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31086">
        <property type="entry name" value="ALUMINUM-ACTIVATED MALATE TRANSPORTER 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11744">
        <property type="entry name" value="ALMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0407">Ion channel</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="75"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="100"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="125"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="155"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7739498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="387" mass="43228" checksum="955AED928822CDDF" modified="2024-05-29" version="1">MSTTIDIPNENEASHNHEKQNKLFFVSNVWYSIAKYHEQHERKVIHSIKVGTALVLVSLLYLLDPLFKHVGESSMWAIMTVVLIYDFFAGATLSKGILRGIGTILGGVVAQYCRMIPSIKRKCDYGIMIYILTFNLVAVSGLRADRIWELARLRLIAIAMGVAIVIITNLLIYPMWAGDELHNLTSSKFNKLACCIEECMEASFSVGEKKGHLSININGCKSVLNSKSNDESLANFAKWEPWHGKFGFSYPWEKYLKIGELLRELAAIILPLHACIALQLQASTTLQQPIKETCKNAGLSLGRTMRELGESVLKMQRGQEKVLKIPELQSFKLELTILSTSGLQAIENVEGLAIANILFLLMEIVDKVKVLAKEVEELGEIAGFESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GHB8</accession>
    <name>A0AAD5GHB8_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_024846</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008215">
        <property type="protein sequence ID" value="KAI7741324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GHB8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7741324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="73" mass="7672" checksum="094E94F2E3800697" modified="2024-05-29" version="1" fragment="single">MLRPGLGVATPVPARASFLEVICGHGSCIGAALMRYLLISATYIIGEIMGNMCSSTFGGYEDDPVEGSGRYAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GI98</accession>
    <name>A0AAD5GI98_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GDSL esterase/lipase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_005703</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008340">
        <property type="protein sequence ID" value="KAI7740848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GI98"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01837">
        <property type="entry name" value="SGNH_plant_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051058">
        <property type="entry name" value="GDSL_Est/Lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035669">
        <property type="entry name" value="SGNH_plant_lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45648">
        <property type="entry name" value="GDSL LIPASE/ACYLHYDROLASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_4G14700)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45648:SF169">
        <property type="entry name" value="TRIACYLGLYCEROL LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7740848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="443" mass="49570" checksum="E1D31B4269EC1E32" modified="2024-05-29" version="1" fragment="single">RGLDKPCLFSPFKIVTITIKYFFSSSPRISGMDILWLFLGLILVISVSGSPKVNQPRSFFVFGDSLVDNGNNNFLLTYARADMPPYGIDTPSHRPNGRFSNGLNIPDIISEHLGSEPVLPYLSPEMTDKKLLTGANFASAGIGILNDTGFQFATQGLTLANILRMSLQIEYFKEYQQRISEKQAEELVNKALVLVSLGGNDFVNNYYLTPFSARRQQFALPNYVALLISEYRKIMMTLYESGVRRAILLGSGPLGCAPAELAIHSSNGECAAELQAAAELFEPQLVQLVQEVNAELGDHVFVAANTKLMHHNIITDPQAFGFENSKTACCGQGPFNGLGLCTPWSSLCENRDKFVFWDAFHPTERASRYIVEQMMNGADEYMRPMNISTIMYLDYNIGDGKDLNGFWCVWRGGHLSVYGGSAIDRGSWRRRGFKDRWRLRWKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GJ32</accession>
    <name>A0AAD5GJ32_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CAAX prenyl protease 2/Lysostaphin resistance protein A-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_032684</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007835">
        <property type="protein sequence ID" value="KAI7743019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GJ32"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004175">
        <property type="term" value="F:endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080120">
        <property type="term" value="P:CAAX-box protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003675">
        <property type="entry name" value="Rce1/LyrA-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36736">
        <property type="entry name" value="OS03G0100030 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36736:SF1">
        <property type="entry name" value="OS03G0100030 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02517">
        <property type="entry name" value="Rce1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="239"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="321"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="CAAX prenyl protease 2/Lysostaphin resistance protein A-like" evidence="3">
        <location>
            <begin position="182"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7743019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="367" mass="40229" checksum="8A65CE75D3B00111" modified="2024-05-29" version="1">MEIPMCSRSPTNKLGVGLPDIFYKRRKVVGRKVVTVRATVERNGSDGIDGGGRKNEVAGGFSGSGSGSSSAMEVTTFNQRFPDTEFPVWDKIGAVVRLSYGIGIYGAMGLVGNFICSTTGIDSTGGYSPSLDAIVQGLGYAAPPIMALLFILDDEVVKVSPHARAIRDVEDEELRSFFYGMSPWQFIMIVAASSVGEELFYRAAVQGALAEVFLRWNDLVTNASGMAALTGVLPPFVPFAQAFAAVITAALTGSLYYVAASPKDPTYVVAPVLKSRSGRQEMKKLFAAWYERRQMKKIYSPLLEGLLALYLGFEWNQTDNILAPMITHGIYSATILGHGLWKIHDHRRRLRQRIQKLNLEENNSRNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GJ79</accession>
    <name>A0AAD5GJ79_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">SUN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_023601</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007799">
        <property type="protein sequence ID" value="KAI7743154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GJ79"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043495">
        <property type="term" value="F:protein-membrane adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045119">
        <property type="entry name" value="SUN1-5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012919">
        <property type="entry name" value="SUN_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12911:SF8">
        <property type="entry name" value="KLAROID PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12911">
        <property type="entry name" value="SAD1/UNC-84-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07738">
        <property type="entry name" value="Sad1_UNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51469">
        <property type="entry name" value="SUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="83"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="SUN" evidence="7">
        <location>
            <begin position="244"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="66"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7743154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="416" mass="46355" checksum="25C933D4E6C92A22" modified="2024-05-29" version="1">MSSSTVSRSNASLRSRPTTDKKPTQDFPTTDDISHSPLATDPTHPTKPIEPDTSKQPPIPTSSTTKLRKRTLVKKPTRPPWKTAASVVVKNLGVLLILLLLFQMVRRLGFNQAGIVSGVFDSDYERRIAEVEAFLKTTTKMMQLQVDVVDRKIETEVKGLKTELSKRIDDEKVAVGQKFGEFDRRVGSVEKMLSGSEWLSKEEFDRFVEEFGKGGGEVKLDEIRGLAKEIVEREIGKHAADGLGRVDYAVWTGGAKVLRHSEAFSASRVMGWFSGNVRSDAVKVLQPSFGQPGECFPLKGDNGFVDVKLRTAIVPEAVTLEHVSKSVAFDISSAPKHCKVWGWLQNDEDTQKEHLLTEFTYDLEKSIAQTFNVLDTKGEDQIVIDTIRLELLSNHGSPTHTCIYRVRVHGHQPNDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GJG0</accession>
    <name>A0AAD5GJG0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Formin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_017790</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the formin-like family. Class-II subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007362">
        <property type="protein sequence ID" value="KAI7745090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GJG0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.2220:FF:000020">
        <property type="entry name" value="Formin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2220">
        <property type="entry name" value="Formin, FH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015425">
        <property type="entry name" value="FH2_Formin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042201">
        <property type="entry name" value="FH2_Formin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051144">
        <property type="entry name" value="Formin_homology_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45733">
        <property type="entry name" value="FORMIN-J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45733:SF17">
        <property type="entry name" value="FORMIN-LIKE PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02181">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00498">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51444">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FH2" evidence="5">
        <location>
            <begin position="96"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="143"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="477"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="376"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="68"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="146"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="477"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="502"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU361260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7745090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="533" mass="57913" checksum="F50E2580A7DC7C17" modified="2024-05-29" version="1" fragment="single">APPPPPPPPGRNGPGAPPPPPPPSRNGPGAPPPPPPPGARPGVPGPPPPPGGSGGPAPPPPPGPKASNGLQQPTPSSGRFRASGPPSSGKGRGGVGSSIPPKKTSLKPLHWVKVTRAMQGSLWADNQKPEDQSRAPEIDISELESLFSTPSVSDNTNKGGARRGSKINKPEKVQLVDLRRAYNCEIMLTKIKIPLPDMLNAILALDASALDIDQVENLIKFCPTKEEMETLKNYTGNKEMLGKCELFFMELMKVPRVESKLRVFAFTITFTSQVSDLRRNLSAINDATKEVKESAKLRQIMQTILTLGNALNQGTARGSAIGFKLDSLLKLSDTRARNNKMTLMHYLCKLLAEKMPELLDFDKDLVHLEAASKIQLKTLAEEMQAVSKGLEKVEQELTASENDGAISAGFQKALKGFLDTAEAEVRALISLYTEVGRNADSLSQYFGEDPVRCPFEQVTQILAVFAKMFKKARDENEQQAEAEKKRLEKEALKEQSSVNHTSRKDGVDVDSQKDLRNMIRDRSLSIKKRDAGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GJG8</accession>
    <name>A0AAD5GJG8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Choline transporter-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_027093</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Choline transporter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the CTL (choline transporter-like) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007357">
        <property type="protein sequence ID" value="KAI7745120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GJG8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007603">
        <property type="entry name" value="Choline_transptr-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04515">
        <property type="entry name" value="Choline_transpo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU368066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7745120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="115" mass="13149" checksum="152CB3D398DE7F08" modified="2024-05-29" version="1" fragment="single">TRVIDLVRDFVHVANFPSKSETRENLPTDEDMRLNCRKLKELNAKKEKVQVKEEPQNEKSGVCWGLGYIIATLFLAVVEMSIDTIILSYCQDAEEHQGTAHYAPPPLIETFDEQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GKD9</accession>
    <name>A0AAD5GKD9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">3-oxoacyl-[acyl-carrier-protein] synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">M8C21_013546</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">AA19_3_7</strain>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Specific for elongation from C-10 to unsaturated C-16 and C-18 fatty acids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a fatty acyl-[ACP] + malonyl-[ACP] + H(+) = a 3-oxoacyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:22836"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:9916"/>
            <dbReference type="Rhea" id="RHEA-COMP:14125"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78776"/>
            <dbReference type="ChEBI" id="CHEBI:138651"/>
            <dbReference type="EC" id="2.3.1.41"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15 17">Belongs to the thiolase-like superfamily. Beta-ketoacyl-ACP synthases family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007561">
        <property type="protein sequence ID" value="KAI7744249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GKD9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004315">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00834">
        <property type="entry name" value="KAS_I_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000027">
        <property type="entry name" value="3-oxoacyl-[acyl-carrier-protein] synthase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017568">
        <property type="entry name" value="3-oxoacyl-ACP_synth-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000794">
        <property type="entry name" value="Beta-ketoacyl_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018201">
        <property type="entry name" value="Ketoacyl_synth_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014031">
        <property type="entry name" value="Ketoacyl_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014030">
        <property type="entry name" value="Ketoacyl_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020841">
        <property type="entry name" value="PKS_Beta-ketoAc_synthase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03150">
        <property type="entry name" value="fabF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004970">
        <property type="entry name" value="PRK06333.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005589">
        <property type="entry name" value="PRK07314.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11712:SF336">
        <property type="entry name" value="3-OXOACYL-[ACYL-CARRIER-PROTEIN] SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11712">
        <property type="entry name" value="POLYKETIDE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00109">
        <property type="entry name" value="ketoacyl-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02801">
        <property type="entry name" value="Ketoacyl-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000447">
        <property type="entry name" value="KAS_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00825">
        <property type="entry name" value="PKS_KS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00606">
        <property type="entry name" value="KS3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52004">
        <property type="entry name" value="KS3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="11 15" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="4 15" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-0934">Plastid</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 15 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Ketosynthase family 3 (KS3)" evidence="19">
        <location>
            <begin position="51"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="7"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="active site" description="For beta-ketoacyl synthase activity" evidence="16">
        <location>
            <position position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00058711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR000447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR000447-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS52004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAI7744249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="464" mass="49651" checksum="51F529DBC42206B1" modified="2024-05-29" version="1">MQAQALLQSTHLGFSKPNPFQTRPTTKINPPTRRPTIIMSATAPKREKDPKKRVVITGMGLVSVFGNDVDTYYDKLLAGESGISLIDRFDASKFPTRFGGQIRGFKSDGYIDGKNDRRLDDCLRYCIVAGKKALEDADLGGDKLSKIDKERAGVLVGTGMGGLTVFSDGVQNLIEKGYRKITPFFIPYAITNMGSALLAIDLGFMGPNYSISTACATSNYCFYAAANHIRRGEADMMIAGGTEAAIIPIGLGGFVACRALSQRNDDPQTASRPWDKDRDGFVMGEGAGVLVMESLEHAMKRGAPIIAEYLGGAVNCDAYHMTDPRSDGLGVSSCIQSSLEDAGVSPEEVNYINAHATSTLVGDLAEVNAVKKVFKSTDGIKMNSTKSMIGHCLGAAGGLEAIATVKAIQTGWLHPTINQFNPEPAVEFDTVANQKQQHEINVAISNSFGFGGHNSVVAFSAFKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GKY6</accession>
    <name>A0AAD5GKY6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cytochrome P450 704C1-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_032771</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006895">
        <property type="protein sequence ID" value="KAI7746785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GKY6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11064">
        <property type="entry name" value="CYP86A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24296">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0349">Heme</keyword>
    <keyword evidence="5 6 7" id="KW-0408">Iron</keyword>
    <keyword evidence="3 6 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042204576" description="Cytochrome P450 704C1-like protein" evidence="8">
        <location>
            <begin position="21"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="6">
        <location>
            <position position="443"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7746785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="499" mass="57176" checksum="49F1EA32E94EBF6B" modified="2024-05-29" version="1" fragment="single">TTLLLIVLAILSLILKIFIGKSIKSPQHAPVVGTVFDQLFYFSKLHDYHADVALKHPTFRLLAPDQSELYTTDTRNIEHILKTNFDNYSKGESNKDIITDLFGNGIFAVDGVMWKQQRKLASFEFSTRVLRDFSCSVFRKNAVKLVGVVSKFSEANKDFDVQNLLMQSALDSIFKVGFGVDLNCLEGSSKEGSAFINAFDDSNALTYWRYVDPIWKLKRFLNIGCEAALRNNIRLINNFVLDLISKRRKQLETNQHCNEREDILSRFLIESKKDAAMNDRYLMDIILNFILAGKDSSANTLSWFFYMLCKNPLVQDKVVSEIEKVVGQQEKGSRIEDFVNKIDDQVLEKMHYLHAALTETLRLYPAVPVDGRVADADDILPDGFKLKKGDGVYYISYAMGRMPYIWGDDAYDFKPERWLNDNGVFQPESPFKFIAFHAGPRICLGKDFAYRQMKIVSIALLHFFMFKLADESRKVTYRTMFTLHIDGGLHLLAFPRISA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GLG5</accession>
    <name>A0AAD5GLG5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UspA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_007990</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006771">
        <property type="protein sequence ID" value="KAI7747357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GLG5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23659">
        <property type="entry name" value="USP_At3g01520-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31964">
        <property type="entry name" value="ADENINE NUCLEOTIDE ALPHA HYDROLASES-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31964:SF131">
        <property type="entry name" value="UNIVERSAL STRESS PROTEIN A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="1">
        <location>
            <begin position="12"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7747357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="174" mass="19235" checksum="BD1F651578A9F455" modified="2024-05-29" version="1">MEPTIGGDKTMMKIWIAVDESEGSFYALEWALRHLFLRHDATDKVSALNVTVVHVQPPFQPTYTALSTGPVLFSTSEARESVMKAEEESAAKVIARASELCDQHKIKAEFLVLKGKPKEILVESVEQMDIDLLVVGSRGLGQIKRAVLGSISDYCAHHANCPVLIVRFPKESEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GLZ1</accession>
    <name>A0AAD5GLZ1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_032913</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007352">
        <property type="protein sequence ID" value="KAI7745136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GLZ1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="13"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7745136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="62" mass="6779" checksum="627BCEB45ABA275D" modified="2024-05-29" version="1" fragment="single">MNKRPPPDPVAVLRGHRDSVMDVCFHPSQNLVFSGSSDGELRIWDGVLRISRLCSGSVDSLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GME3</accession>
    <name>A0AAD5GME3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ankyrin repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_028959</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007225">
        <property type="protein sequence ID" value="KAI7745496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GME3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24177">
        <property type="entry name" value="CASKIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24177:SF443">
        <property type="entry name" value="PGG DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="393"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7745496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="421" mass="48217" checksum="7BFDDDE9AE2DA535" modified="2024-05-29" version="1">MGDWETAKLIFDKRPDMVRIGLSKSFATALHVAVTANETKQTLHFVKELVNMMTIEELEIQNKSSNTAFWLACASGNTEMAKILMEKNPNLLNIRGCEGDLPLAASAFTGRYNLVKHLYHHSHKMTGDYWTNDDRSFILLQCVERDFYDIALQIVNDRPELPLDVGRLLEVLARKPDALNNVQNDLITRIIKSTFRFFQMKLQPHVAEVDTDALKLLKHIWGHATRTMNLVEIQDMLKGPPRMLFVAAEMGNTRFIVELVRTYPDLMFNTNEDMLNYGLTIFHIAVMRRHQGIYNLLYEIGKSNNDVCIVLDMSTDNNMLHLVGKTSKEMAAKTPGASLLMQRELLWFKEVEKMMPPYMREETNNDGLTPYELFSKENVDQVSKELKWTKDCMVVTTLIVTVAFAAAFTMPFVCSLPQLHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GNE9</accession>
    <name>A0AAD5GNE9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="3">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_024663</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010007046">
        <property type="protein sequence ID" value="KAI7746098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GNE9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000050">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024729">
        <property type="entry name" value="USP7_ICP0-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029346">
        <property type="entry name" value="USP_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14533">
        <property type="entry name" value="USP7_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12436">
        <property type="entry name" value="USP7_ICP0_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin carboxyl-terminal hydrolase 7 ICP0-binding" evidence="8">
        <location>
            <begin position="21"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin carboxyl-terminal hydrolase C-terminal" evidence="9">
        <location>
            <begin position="353"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF14533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7746098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="486" mass="57206" checksum="C1E6E7D747BF4D68" modified="2024-05-29" version="1">MTFNCFKEEVAKAFGIPVQFQRFWLWAKRQNQTYRPNRPLTPDEETLTVGQLRDTFNKIQNAELRLFLETEFGPDLNPLPLPEKTKDEILLFFKLYDPEKLELRSFVKSSGKVIEIIPKLKEIAGFSSDEEIKLYEEIKFEPTIMCEPLDKGASFRSCQIEDGDIICFQKLQQTNHVEKYQYPDVPSFFNYVKNRQIVHFRSIDRPKEDDFCLELSKSHTYDDVVERVAYKLGLDDPSKIRLTPHNCYSQQPKPNPIKYRLADLLDMLVHHNQMTDILYYEVLDIPLPQLQCLKTLKIVFLPASKEEPVIHNIRLPKQSTVGDVLDELKTKVELSHRDAELRLLEVFVHKIYKIPEEEKNLGPQDRLIHVYHFNKEANQVQVQHFGDPFFLVIHDDETLADVKTRIQKKLQVPDDEFSKWKFAFLSIGRTEYLEDTDIVSSRFQSKNVYGAWENYLGLEHPDTTPKRSFTANQNRHTYERAVKIYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GNK9</accession>
    <name>A0AAD5GNK9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">RNA-binding protein 8A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">M8C21_021929</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">AA19_3_7</strain>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RBM8A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006341">
        <property type="protein sequence ID" value="KAI7749562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GNK9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12324">
        <property type="entry name" value="RRM_RBM8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000525">
        <property type="entry name" value="RNA-binding protein 8A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008111">
        <property type="entry name" value="RNA-bd_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033744">
        <property type="entry name" value="RRM_RBM8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45894">
        <property type="entry name" value="RNA-BINDING PROTEIN 8A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01738">
        <property type="entry name" value="RNABINDINGM8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="11" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="10" id="KW-0866">Nonsense-mediated mRNA decay</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 14" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="8" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="RRM" evidence="16">
        <location>
            <begin position="94"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="29"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="176"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="8"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="59"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00077711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAI7749562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="198" mass="22053" checksum="150697434B12A77E" modified="2024-05-29" version="1">MANAADVDALDFEPDDDDLMDEDATVDVDASSPTTAAAHIPKLKSAITSSAPKKTKGRGFREETDPGRTNRMSGRFDSLDSDGGPGPERSIEGWIILVTGVHEEAQEDDLQNAFGEFGEIKNLHLNLDRRTGFVKGYALIEYENYEEAEKAIASMDGGELLTQTVNVDWAFSRGPFKRRNDRRRSPHGHRSRSPRRRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GPP7</accession>
    <name>A0AAD5GPP7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_013064</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006093">
        <property type="protein sequence ID" value="KAI7750762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GPP7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.10">
        <property type="entry name" value="Hypothetical protein af1432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43061">
        <property type="entry name" value="GTP DIPHOSPHOKINASE RSH1, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43061:SF1">
        <property type="entry name" value="GTP DIPHOSPHOKINASE RSH1, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7750762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="170" mass="18487" checksum="63B3FFBE988E2FFE" modified="2024-05-29" version="1" fragment="single">MASAPFLSVSLECVSVCKYPKGDGSGSGSGRGRYDCSPVTCAWKAPRALTGSFASTANGQSERRIRHVHRCGALDHLPSRRPGRSKLIHLACKKWEVGSSSSFSSGYDEVSPEHLWEDLKPAIAYLTSDELKLVHDALNLAFEAHNGQKRRSGEPFIIHPVEVAKILGEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GPW9</accession>
    <name>A0AAD5GPW9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_030732</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006085">
        <property type="protein sequence ID" value="KAI7750790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GPW9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031956">
        <property type="term" value="F:medium-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05926">
        <property type="entry name" value="FACL_fum10p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.30:FF:000007">
        <property type="entry name" value="4-coumarate--CoA ligase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045310">
        <property type="entry name" value="Pcs60-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43201">
        <property type="entry name" value="ACYL-COA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43201:SF5">
        <property type="entry name" value="MEDIUM-CHAIN ACYL-COA LIGASE ACSF2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="5">
        <location>
            <begin position="171"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="6">
        <location>
            <begin position="367"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7750790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="466" mass="50418" checksum="B8F596FC4ADDE64B" modified="2024-05-29" version="1">MDTPTQTLTGLLKHVAGIFPDRRAISVSGKFDITHSRLNQLIDHAAGLLVAAGVKPHDVVALTFPNTVEYVIMFLAVIRARATAAPLNQAYTTDEFEFYLSDSESKLLLTAKEGNEAAEKAASKTGIRHITATLNDVASAIHLSTVTNTDTDTDTIVNEPSDVALFLHTSGGAVTLPSAGRFSATSFWTDMIKYNATWYTAVPTIHQIILDRHLSKPEPVYPKLRFIRSCSASLAPSILARLEEAFNAPVLEAYAMTEATHLMASNPLPENGPHVPGSVGKPVGQEMAILDENGVEQKAGANGEVCIRGPNVTSGYKNNPEANKSAFLFGWFHTGDIGYFDSDGYLHLVGRIKELINRGGEKISPIEVDAVLLSHPDVAQAVCFGVPDDKYGEEINCAVIPREGSGLDEEEVLRFCKKNLAAFKVPKKVFITDSVPKTATGKIQRRIVAEHFLTHISTAKVPKFGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GQC1</accession>
    <name>A0AAD5GQC1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">ATP-dependent 6-phosphofructokinase</fullName>
            <shortName evidence="14">ATP-PFK</shortName>
            <shortName evidence="14">Phosphofructokinase</shortName>
            <ecNumber evidence="14">2.7.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Phosphohexokinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">PFK</name>
        <name evidence="17" type="ORF">M8C21_007838</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">AA19_3_7</strain>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 14">Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>beta-D-fructose 6-phosphate + ATP = beta-D-fructose 1,6-bisphosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16109"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="14">Allosterically activated by AMP.</text>
    </comment>
    <comment type="pathway">
        <text evidence="14">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 14">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the phosphofructokinase type A (PFKA) family. PPi-dependent PFK group II subfamily. Atypical ATP-dependent clade 'X' sub-subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.11" evidence="14"/>
    <dbReference type="EMBL" id="JAMZMK010006603">
        <property type="protein sequence ID" value="KAI7748108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GQC1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003872">
        <property type="term" value="F:6-phosphofructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006002">
        <property type="term" value="P:fructose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.450:FF:000002">
        <property type="entry name" value="ATP-dependent 6-phosphofructokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.460:FF:000018">
        <property type="entry name" value="Phosphofructokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01981">
        <property type="entry name" value="Phosphofructokinase_II_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022953">
        <property type="entry name" value="ATP_PFK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050929">
        <property type="entry name" value="PFKA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000023">
        <property type="entry name" value="Phosphofructokinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035966">
        <property type="entry name" value="PKF_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012004">
        <property type="entry name" value="PyroP-dep_PFK_TP0108"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005301">
        <property type="entry name" value="PRK06830.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45770">
        <property type="entry name" value="ATP-DEPENDENT 6-PHOSPHOFRUCTOKINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00365">
        <property type="entry name" value="PFK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00476">
        <property type="entry name" value="PHFRCTKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53784">
        <property type="entry name" value="Phosphofructokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 14" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="10 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12 14" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="9 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphofructokinase" evidence="16">
        <location>
            <begin position="135"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="14">
        <location>
            <position position="260"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="204"/>
            <end position="205"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="229"/>
            <end position="232"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="258"/>
            <end position="260"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="303"/>
            <end position="305"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="359"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="416"/>
            <end position="419"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Important for substrate specificity; cannot use PPi as phosphoryl donor" evidence="14">
        <location>
            <position position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAI7748108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="529" mass="58214" checksum="972D236F1CA3845E" modified="2024-05-29" version="1">MHSVSNSGAIKLSSGNNINQKKDNRKHKVKITHQTPVMGQLPATTSSQPKVDTGDHGYILEDVPHLTDYIPHLPTYPNPLQDNPAYAVVKQYFVDTDDTVAQKIVVHANSPRGIHFRRAGPRQKVYFKSDDVHACIVTCGGLCPGLNTVIREIVGSLHDMYGVKRIEGIEGGYQGFYSRNTIQLTPKKVNGIHKRGGTIIGTSRGGYDNKKIVNSIQDRGINQVYIIGGDGTQKGASAIYEEVRKRGLQVAVVGIPKTIDNDIPVIDQSFGFDTAVEEAQRAINAAHVEAESNENCIGLVKLMGRNSGFIAMYATLASRDVDCCLIPESPFYLEGPGGLFEYIEKRIKENGHMVIVVAEGAGQDLVAESLRANDNKDASGNKLLDDVGLWLSQKIKDHFKKQTEMEINLKYIDPTYMIRAVPSNASDNVYCTLLAQSAVHGAMSGYTGFTVGPVHGRECYLPFYRITEKQNKVVITDRMWARMLSSTHQPSFLSTKDIIESFTDEAPPTQLLDDQVADNSLVTKETTIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GQL7</accession>
    <name>A0AAD5GQL7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Carbohydrate kinase PfkB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_015686</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the carbohydrate kinase PfkB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005827">
        <property type="protein sequence ID" value="KAI7751737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GQL7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01168">
        <property type="entry name" value="adenosine_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052700">
        <property type="entry name" value="Carb_kinase_PfkB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43320:SF1">
        <property type="entry name" value="OS01G0105900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43320">
        <property type="entry name" value="SUGAR KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carbohydrate kinase PfkB" evidence="4">
        <location>
            <begin position="77"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7751737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="367" mass="38674" checksum="E63058E3F73DC950" modified="2024-05-29" version="1">MVAEFLPPHNSGDSASAPVILGLLPAALVDHIARVDSSILSSIPGEPGGSFPVAAEELKHILKEVDTHILSSPNDLSPIKTIAGGSVTNTIRGLAAGFGISCGVIGACGDDEQGALFLENMSLCGVNISRLRVKDEHTGQCICLVDPLGNRTMRPCLATAAKVQAGDLKREDFKGSKWLVMRYSIYNLETINAAIKIAKQEGVLISLDLASFEMVRKFRAPLLQLLESGNIDLCFANEDEAAELLSGEQAAQPEAALDMLGKYCEWAVVTLGANGCIARHNKEVVRVPAVGQTKAIDATGAGDLFAGGFLYGLVKGLSLEECCIVGSCSGGSVVRSLGGEVSAENWQWMYKQLKTNGLSAPAVVHNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GR20</accession>
    <name>A0AAD5GR20_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_008337</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the peroxisomal membrane protein PXMP2/4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005738">
        <property type="protein sequence ID" value="KAI7752172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GR20"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007248">
        <property type="entry name" value="Mpv17_PMP22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266:SF80">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 2, PXMP2 MPV17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04117">
        <property type="entry name" value="Mpv17_PMP22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="108"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="141"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="208"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="35"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="37"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7752172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="231" mass="25040" checksum="081312607586C84B" modified="2024-05-29" version="1" fragment="single">ATASVTNNGLRPCLFQFNHRKIELGRKGFRVEATDSSGGGYGGSDSGGGGGGGGGDGAGGNKWSYLSLLEAHPVLTKAVTSALLTFVGDLICQVLIDKVPSLDLKRVSLFTFLGMALVGPTLHFWYLYLSKLVTMTGAPGAFLRLIIDQFIFAPAFIGVFLSTLVTLEGRPSQVLPKLQQEWVSSVVANWQLWIPFQFLNFLFVPQQFQVLAANFIALVWNVILSYKAHKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GUL5</accession>
    <name>A0AAD5GUL5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SKP1-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_000080</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in ubiquitination and subsequent proteasomal degradation of target proteins. Together with CUL1, RBX1 and a F-box protein, it forms a SCF E3 ubiquitin ligase complex. The functional specificity of this complex depends on the type of F-box protein. In the SCF complex, it serves as an adapter that links the F-box protein to CUL1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 6">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of a SCF (SKP1-cullin-F-box) protein ligase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the SKP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005584">
        <property type="protein sequence ID" value="KAI7752921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GUL5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009867">
        <property type="term" value="P:jasmonic acid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18322">
        <property type="entry name" value="BTB_POZ_SKP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000057">
        <property type="entry name" value="SKP1-like protein 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016897">
        <property type="entry name" value="SKP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001232">
        <property type="entry name" value="SKP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036296">
        <property type="entry name" value="SKP1-like_dim_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016072">
        <property type="entry name" value="Skp1_comp_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016073">
        <property type="entry name" value="Skp1_comp_POZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11165">
        <property type="entry name" value="SKP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01466">
        <property type="entry name" value="Skp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03931">
        <property type="entry name" value="Skp1_POZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028729">
        <property type="entry name" value="E3_ubiquit_lig_SCF_Skp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00512">
        <property type="entry name" value="Skp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81382">
        <property type="entry name" value="Skp1 dimerisation domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="SKP1 component POZ" evidence="8">
        <location>
            <begin position="5"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="SKP1 component dimerisation" evidence="7">
        <location>
            <begin position="106"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR028729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7752921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="155" mass="17466" checksum="2ACD2B68928F4644" modified="2024-05-29" version="1">MSSSKTIVLRSSDGETFEVDEAVAVESQTIKHMIEDGCADTSIPLPNVTSKILSKVIEYCKKHVETPNADEKVAEEDLKSFDADFVKVDQSTLFDLILAANYLNIKSLLDLTCQTVADMIKGKTPEEIRKTFNIKNDFTPEEEEEVRRENAWAFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GUN7</accession>
    <name>A0AAD5GUN7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Growth-regulating factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_026707</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Transcription activator.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="3">The QLQ domain and WRC domain may be involved in protein-protein interaction and DNA-binding, respectively.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the GRF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005567">
        <property type="protein sequence ID" value="KAI7752966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GUN7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032502">
        <property type="term" value="P:developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031137">
        <property type="entry name" value="GRF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014977">
        <property type="entry name" value="WRC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31602">
        <property type="entry name" value="GROWTH-REGULATING FACTOR 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31602:SF8">
        <property type="entry name" value="GROWTH-REGULATING FACTOR 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08879">
        <property type="entry name" value="WRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51667">
        <property type="entry name" value="WRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0010">Activator</keyword>
    <keyword evidence="1 2 3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="WRC" evidence="5">
        <location>
            <begin position="12"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="39"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="244"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Bipartite nuclear localization signal" evidence="2">
        <location>
            <begin position="17"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Bipartite nuclear localization signal" evidence="2">
        <location>
            <begin position="45"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="42"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="62"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="80"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="258"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU367127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7752966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="284" mass="31498" checksum="14E4EF3272B989F0" modified="2024-05-29" version="1">MGYCTYYGKKFDPEPGRCRRTDGKKWRCAKDAYPDSKYCERHMHRGRNRSRKPVESQSASQSLSTAVSRVTAVSSTGSGNRSYQNLSNGSFQNSQLYPTSNSGTIDFGSNASKLQVHASAYGINNSFRYDQGLTINVDDINYSSGASSARGVAMESNADSPWRLVPTQISSNSLMESRNDSYLQTKSPQLTMVNAFEPVIDATTPGSKPSQQHCLFGSKIESPVEVKHEQQHMMRPFFDEWPEQREPWSSLDGAGGKNSFSTTRLSMSTAHDYSARSDCTPNDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GW82</accession>
    <name>A0AAD5GW82_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">beta-aspartyl-peptidase</fullName>
            <ecNumber evidence="3">3.4.19.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_027731</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleavage of a beta-linked Asp residue from the N-terminus of a polypeptide.</text>
            <dbReference type="EC" id="3.4.19.5"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterotetramer of two alpha and two beta chains arranged as a dimer of alpha/beta heterodimers.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.5" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010001136">
        <property type="protein sequence ID" value="KAI7755424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GW82"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004067">
        <property type="term" value="F:asparaginase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008798">
        <property type="term" value="F:beta-aspartyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04701">
        <property type="entry name" value="Asparaginase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.30:FF:000001">
        <property type="entry name" value="Isoaspartyl peptidase/L-asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.30">
        <property type="entry name" value="(Glycosyl)asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000246">
        <property type="entry name" value="Peptidase_T2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10188:SF13">
        <property type="entry name" value="ISOASPARTYL PEPTIDASE_L-ASPARAGINASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10188">
        <property type="entry name" value="L-ASPARAGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01112">
        <property type="entry name" value="Asparaginase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="7">
        <location>
            <position position="196"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="224"/>
            <end position="227"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="246"/>
            <end position="249"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Cleavage; by autolysis" evidence="9">
        <location>
            <begin position="195"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR600246-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR600246-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR600246-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7755424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="326" mass="34419" checksum="F668BA9441924396" modified="2024-05-29" version="1">MVGWAIAVHGGAGVDPNLPTERQEQAKQLLTRCLNLGISALRSSASAIDVVELVVRELETDPIFNSGRGSALTEKGTVEMEASIMDGVGRRCGAVSGLSTVKNPVSLARLVMDKSPHSYLAFSGAEDFAKQMGVEREENDYFVTEDNVGMLKVAKEANSIVFDYRIPTTGYESCGAGVESPIRMNGLPISVYAPETVGCVVVDSQGRCAAATSTGGLMNKRIGRIGDSPLIGAGTYACDMCGVSCTGEGEAIIRGTLAREVAAVMEYKGLALQEAVDFVIKERLDEGQAGLIAVSKTGEVAYGFNCVGMFRGCATEDGFMEVGIWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GWQ2</accession>
    <name>A0AAD5GWQ2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">60S ribosomal protein L36</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_006533</name>
        <name evidence="7" type="ORF">M8C21_006534</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL36 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010001845">
        <property type="protein sequence ID" value="KAI7755066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAMZMK010001845">
        <property type="protein sequence ID" value="KAI7755067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GWQ2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1760:FF:000001">
        <property type="entry name" value="60S ribosomal protein L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1760">
        <property type="entry name" value="60S ribosomal protein L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000509">
        <property type="entry name" value="Ribosomal_eL36"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038097">
        <property type="entry name" value="Ribosomal_eL36_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10114">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01158">
        <property type="entry name" value="Ribosomal_L36e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01190">
        <property type="entry name" value="RIBOSOMAL_L36E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="79"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="79"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7755066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7755067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="110" mass="12432" checksum="FC5224C3D4BA9869" modified="2024-05-29" version="1">MAPKQPNTGLFVGLNKGHVVTKKELAPRPSDRKGKTSKRVHFVRSLIREVAGFAPYEKRITELLKVGKDKRALKVAKRKLGTHKRAKKKREEMSSVLRKMRSGGGTEKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GXG6</accession>
    <name>A0AAD5GXG6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cytochrome b5 heme-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_022182</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the cytochrome b5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000336">
        <property type="protein sequence ID" value="KAI7756594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GXG6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018506">
        <property type="entry name" value="Cyt_B5_heme-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050668">
        <property type="entry name" value="Cytochrome_b5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19359">
        <property type="entry name" value="CYTOCHROME B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19359:SF95">
        <property type="entry name" value="CYTOCHROME B5 TYPE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00191">
        <property type="entry name" value="CYTOCHROME_B5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0349">Heme</keyword>
    <keyword evidence="3 5" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="84"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="6">
        <location>
            <begin position="123"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7756594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="178" mass="19329" checksum="09E2FD7BA95F2135" modified="2024-05-29" version="1">MPVTATTHQTTTNTHTYTATTHPHMYNGSLLVSVSNGSLSSECTSFSIDSATLSSVYGLPSVCFSSFLSDSSFLTSSINVGMELIIMVTLIIGFFLSFLILLPRFIKSDQTKKVHSDADSKVSKAYSKADVSLHNKRTDCWIIIKEKVYDVTPYVEEHPGGDAILAHAGDDSTEGFFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GYC0</accession>
    <name>A0AAD5GYC0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Inositol-1-monophosphatase</fullName>
            <ecNumber evidence="11">3.1.3.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_014339</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Responsible for the provision of inositol required for synthesis of phosphatidylinositol and polyphosphoinositides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>a myo-inositol phosphate + H2O = myo-inositol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:24056"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17268"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:84139"/>
            <dbReference type="EC" id="3.1.3.25"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 10 11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 11">Polyol metabolism; myo-inositol biosynthesis; myo-inositol from D-glucose 6-phosphate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the inositol monophosphatase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.25" evidence="11"/>
    <dbReference type="EMBL" id="JAMZMK010000125">
        <property type="protein sequence ID" value="KAI7757434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GYC0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008934">
        <property type="term" value="F:inositol monophosphate 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006021">
        <property type="term" value="P:inositol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01639">
        <property type="entry name" value="IMPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.540.10:FF:000004">
        <property type="entry name" value="Inositol-1-monophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.80:FF:000002">
        <property type="entry name" value="Inositol-1-monophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.540.10">
        <property type="entry name" value="Fructose-1,6-Bisphosphatase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033942">
        <property type="entry name" value="IMPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020583">
        <property type="entry name" value="Inositol_monoP_metal-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020552">
        <property type="entry name" value="Inositol_monoPase_Li-sen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000760">
        <property type="entry name" value="Inositol_monophosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020550">
        <property type="entry name" value="Inositol_monophosphatase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20854">
        <property type="entry name" value="INOSITOL MONOPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20854:SF4">
        <property type="entry name" value="INOSITOL-1-MONOPHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00459">
        <property type="entry name" value="Inositol_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00377">
        <property type="entry name" value="IMPHPHTASES"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00378">
        <property type="entry name" value="LIIMPHPHTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56655">
        <property type="entry name" value="Carbohydrate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00629">
        <property type="entry name" value="IMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00630">
        <property type="entry name" value="IMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0452">Lithium</keyword>
    <keyword evidence="8 10 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="10">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00055312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR600760-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU364068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7757434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="270" mass="28977" checksum="29F70A016414121C" modified="2024-05-29" version="1">MAGNDSLNEFLTVAVDVAKQAGEVIREGFYKTKNVEHKGTVDLVTETDKACEDLIFDHLKQHFPGHKFIGEETTAAHGVSQLTDEPTWIVDPLDGTTNFVHGFPFVCVSIGLTIKKVPVVGVVYNPIMNELFTAIDGKGAFLNGNPIKVSSHSELVTCLLTTEAGTKRDKATLDATTNRINSLLYKVRSIRMEGSCALGLCGVACGRIDLFYELGFGGPWDVAAGVVIVKEAGGVIFDPSGSDFDITAQRIAASNPLVKEAFIEALQQSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BLT8</accession>
    <name>A0AAD5BLT8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_014584</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011864">
        <property type="protein sequence ID" value="KAI7725762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BLT8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.1190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045124">
        <property type="entry name" value="Su(sable)-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13119:SF12">
        <property type="entry name" value="PROTEIN SUPPRESSOR OF SABLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13119">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="344"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="373"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="398"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="344"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="373"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="398"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="237"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="424"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="433"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7725762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="643" mass="71640" checksum="D74DA7D893DACCC0" modified="2024-05-29" version="1">MIQMDTSSVNRRRQPLRSRTVDTLINILSICYDNSCHIIKNTGKGTEKDVSSDINDIIPKDMLFQEQMPMDETQVVFDSQKGGLCSKLMESAEHDHNLLGEKKDLFNKVNTGHLPTDKDMTSSSPHLPTNKDVEELEVLEDLIDFMPRVDAKSVDKTDSKPKDFIGTKTGPANDNNAIRLLRESVENSGKDMAGLVNKTTRNLVDYSEIGFGSDHVLPLQSNSKIDATKNRDRVYPLKESGAQNNKKRPGSCTKEERTIKKSKKSVDSTTRPASLTLLGEQLANASQEVVDASANKDATVEKKRKRVLTKERKAKRKIKDRIKRAEKNRKLGVKRLKLQPVIKEKKITYCRHYMMGRCHEGEKCKFSHDTTPLTKSKPCCHFARHACMKGDDCPFDHDLSKYPCNNYQTKGSCNRGSDCMFSHEAQPSEAPLNASNESKPEQKPSSNSGSKIVETKSCSVPKFVDVTPKSARPPKGINFLSLEKLPQESRELDKITKTPHVIQVSKEIAKTPHVQDPKEIAKTPNAVQDTSEITKVPPVVPRGINFLSFGKKPGMGHSNDGFPFKISNGTEKPRLSDVEGESLKTTPMVNVVNEVKVDFPTDRPNSSKKPMPIMSFMSSTSQKALHSTLAFASKFDSGVKSKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BMU4</accession>
    <name>A0AAD5BMU4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="1">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_026214</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="1"/>
    <dbReference type="EMBL" id="JAMZMK010012020">
        <property type="protein sequence ID" value="KAI7725183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BMU4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009543">
        <property type="term" value="C:chloroplast thylakoid lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044180">
        <property type="entry name" value="FKBP18-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47862">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FKBP18, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47862:SF2">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase FKBP-type" evidence="2">
        <location>
            <begin position="127"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7725183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="234" mass="24801" checksum="4C6171B683F19F3E" modified="2024-05-29" version="1">MELSLSPYKPSFLIPSSITRKALPHKIKFALACNNYNNNNISSSSEAPVPVVSNNNAPFSESDGRRALMTSFLAAAAAGLCVCDAAGAVSTSRRALKGAKVPESEYTTLPNGLKYYDLKVGNGAEAVKGSRVHYVAKWRNITFMTSRQGLGVGGGTPYGFDVGESERGNVLKGLDLGVQGMRVGGQRLLIVPPELAYGSKGVQEIPPNSTIEIDIELLSIKQSPFGTPVKIVEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BN18</accession>
    <name>A0AAD5BN18_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_016953</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011676">
        <property type="protein sequence ID" value="KAI7726407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BN18"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057075">
        <property type="entry name" value="bHLH_IRO3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47075">
        <property type="entry name" value="TRANSCRIPTION FACTOR BHLH47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47075:SF9">
        <property type="entry name" value="TRANSCRIPTION FACTOR BHLH47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23177">
        <property type="entry name" value="bHLH_IRO3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="8">
        <location>
            <begin position="29"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="149"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="69"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="24"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7726407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="187" mass="20771" checksum="AEFCF56AE0B989E6" modified="2024-05-29" version="1">MKGSVPTEGNVDKANKKSSCSKKNGAKVHRKIHKAEREKLKRDHMNDLFLDLTNALESANQNTGKSSALTDTIRIIQDLQAQVDSLKKENSIGVENDELKEENSVIEGHIKKLQSQIDERVHPQSLWASECNPVVGPVIVLPLQNDPKIPEAVTKQPGPNVSKPHARYPSPSDSWPFNILSERSRAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BNA9</accession>
    <name>A0AAD5BNA9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Gem-associated protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_008598</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the gemin-2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011726">
        <property type="protein sequence ID" value="KAI7726254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BNA9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032797">
        <property type="term" value="C:SMN complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000387">
        <property type="term" value="P:spliceosomal snRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1070">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035426">
        <property type="entry name" value="Gemin2/Brr1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12794:SF0">
        <property type="entry name" value="GEM-ASSOCIATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12794">
        <property type="entry name" value="GEMIN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04938">
        <property type="entry name" value="SIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="48"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="50"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="71"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00025758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7726254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="501" mass="56645" checksum="30A5982C5C1BD958" modified="2024-05-29" version="1">MALETNTSSDDSIINTLQETIFSDLGLTLETITDNQQPHELNPLIPLSQEPGQKNVTQITNSSTSPQNESFDERKPEQKVGSELGLKRDEKTEIKKEKRHRKRGKVLKKQVNDDFVRQSVCDCDKRGDKVRVVYTREELEGLRDVGIEQQKKMWFEVYCGLGSVVAKEYDNLTGCKHDGEGNYVSFDPRPQFANKKGVAADFLGDGSENIGCEMQNMNQVDSLSGVNVDDFDGEEDYYEDEDSDSEYVGIQRPALYVTGEPDFDSGPPQDGLEYLRRVRWEAKRIPKVKIAKVEKKVITKEQTVYMPNIPDIAACPEHLMPSKEWEDVFLADFSKLRLALSEFESSDSTFSDQVESIPLAQSIMDNVIQENLDFCQTDDETIIVQENLDVNQTEEKATICDWPSLRSIMEMEPRARVTILRKRIASMEQMSYLGRPDCAWLFALCAAIDTPLDADTSASLRCLLRKCATLRAEKASLDDEVIMLNILATISGKYFGQLEAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BR73</accession>
    <name>A0AAD5BR73_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_007128</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011267">
        <property type="protein sequence ID" value="KAI7728133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BR73"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032451">
        <property type="term" value="F:demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006402">
        <property type="term" value="P:mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044842">
        <property type="entry name" value="ALKBH9B/ALKBH10B-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31447:SF0">
        <property type="entry name" value="HYDROXYPROLINE-RICH GLYCOPROTEIN FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31447">
        <property type="entry name" value="HYDROXYPROLINE-RICH GLYCOPROTEIN FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7728133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="157" mass="17385" checksum="3ADB00A3C0411432" modified="2024-05-29" version="1">MFTSDFGLRIRMGSRMVRASTTICAFLLVFIIWGDMSFLSRVALATSSPLGVVGGALATHGLATLVKFTEGQPLMLQLVCYEGKKIFGLKEANMSERVEEKCTKFINTFVALKRPMKGHGREMIQFGSLIVDIPFDSEAINGTSKDWKIEPIPSLFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BRQ5</accession>
    <name>A0AAD5BRQ5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">CRAL-TRIO domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_027646</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SFH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011214">
        <property type="protein sequence ID" value="KAI7728433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BRQ5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011074">
        <property type="entry name" value="CRAL/TRIO_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051026">
        <property type="entry name" value="PI/PC_transfer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45657">
        <property type="entry name" value="CRAL-TRIO DOMAIN-CONTAINING PROTEIN YKL091C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45657:SF40">
        <property type="entry name" value="CRAL-TRIO LIPID BINDING DOMAIN, CRAL_TRIO DOMAIN, CRAL_TRIO DOMAIN SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03765">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01100">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46938">
        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="7">
        <location>
            <begin position="133"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="279"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="39"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="280"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="333"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="349"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7728433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="526" mass="60151" checksum="3F9BA947388893CF" modified="2024-05-29" version="1">MMSESGPERPIRPGSEKIELENSEEEKKARRNSFKQKAINASNKFRTSLSKKSRNSKVMSVVEDEHDAEDLKAVEAFRQVLIPEDLLPSKHDDYHMLLRFLKARNYDLEKAKKMWTDMINWRKEYGTETIIEERDDVLQYYPQGYHGIDKDGRPVYIERLGLVDATKLMQVTSLERYVRYHVREFERTFIDKFPACSIAAKTHIDQSTTILDVQGVGLKSMNKSARDLIQSLQSIDGNNYPETLCRMYIINAGSGFRLLWNTVKTFLDPKTTSKINMVRNGEHKCSSRSGIADERTISEDQSAHKVDAESSGMQKEQTKHSQLAPVHEEETRASTSKSTPTANEPRIRTQVSKKTVESSYAKQVQKVPGPQDYYHVTEAASDGLGNHIFTGMMTFMMGVMTMVRMTKAMPKRVTDTNNYAGPVYEESMRQRPGPYQMQSPGISTAEYLSMMKRLGDLEEKVIILTDRPVEMPPEKEEMLNNALNRIEYLEIELAATKKSIGDTIAQQQEFAAYLEKKKKKKGIFGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BS79</accession>
    <name>A0AAD5BS79_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HMA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_001509</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011360">
        <property type="protein sequence ID" value="KAI7727493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BS79"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7727493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="120" mass="13577" checksum="51869D76E50A0572" modified="2024-05-29" version="1">MSAFKHKEEEEQEKSNNLVKVPPATLASIESLTFPLVQEVVILADYRCKLCKDKVADIVSKLKGDMESMEMMITEKKVIITFAGRYSKAIKTHGKKLQVFGIYKNIVNKVSSVLRYSYAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BVC6</accession>
    <name>A0AAD5BVC6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mitochondrial inner membrane protease subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_001433</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010835">
        <property type="protein sequence ID" value="KAI7730145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BVC6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06530">
        <property type="entry name" value="S26_SPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.109.10">
        <property type="entry name" value="Umud Fragment, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036286">
        <property type="entry name" value="LexA/Signal_pep-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053307">
        <property type="entry name" value="Mitochondrial_IM_protease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019533">
        <property type="entry name" value="Peptidase_S26"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47040:SF1">
        <property type="entry name" value="MITOCHONDRIAL ATP-INDEPENDENT INNER MEMBRANE PROTEASE SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47040">
        <property type="entry name" value="OSJNBA0068L06.9 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51306">
        <property type="entry name" value="LexA/Signal peptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7730145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="205" mass="23509" checksum="814DEC5DB38BC860" modified="2024-05-29" version="1">MVSHTTWLRYLARRFEYSLALSVKSYEHGKITNREVGDNFWKTFFQGKLTFLHWNKGEEMAPTIAPEGGTLLVRKLPAADPQRVYVGDVVVMKDPEKSDSYLVRRLAAIEGYEMVSKDEKDEPFVLEKDQCWVLADNDKLKPKEAKDSRLFGPVSMDDIIGRVLYCLRTAVDHGPIQNSQFSMQKDLPVLEVELDVDEMAKNHKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BVU7</accession>
    <name>A0AAD5BVU7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Radical SAM core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_025340</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010782">
        <property type="protein sequence ID" value="KAI7730395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BVU7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000164">
        <property type="entry name" value="23S rRNA methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040072">
        <property type="entry name" value="Methyltransferase_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004383">
        <property type="entry name" value="rRNA_lsu_MTrfase_RlmN/Cfr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30544">
        <property type="entry name" value="23S RRNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30544:SF8">
        <property type="entry name" value="RADICAL SAM SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006004">
        <property type="entry name" value="CHP00048"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00275">
        <property type="entry name" value="adenosine_C2_methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01062">
        <property type="entry name" value="methyltransferase_(Class_A)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Radical SAM core" evidence="11">
        <location>
            <begin position="111"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7730395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="353" mass="38901" checksum="36F65B58BE88E06C" modified="2024-05-29" version="1">MTFRSVFDAPFIRSEFDKSGINTQFIPTIWKYVIQNPNCEWRDIPSLPSAAYSLLSSTFKPCTSSVDSVADSTDQLTTKLLVKLQNGASVETVIMRYDSSLGKYAGNPRSGGPRSTLCVSSQVGCKMGCKFCATGTMGFKSNLSTGEIVEQLVHASRLSPIRNIVFMGMGEPLNNYTALVEAIRVGIIHAINKFHDDLPNLNLAVSLHAPVQDIRCQIMPAARAFPLGKLMDALAEYQRKSGQKIFIEYIMLDGVNDEEQHAHQLGKLLDTFEVVINLIPFNPIGTLSKFGTTGDEKVTIFQRILRNTYNIRTTIRKQMGQDISGACGQLVINLPNKKSASVASLTDIEDLRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BWY7</accession>
    <name>A0AAD5BWY7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_006570</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010526">
        <property type="protein sequence ID" value="KAI7731162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BWY7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46284:SF1">
        <property type="entry name" value="PROTEIN KINESIN LIGHT CHAIN-RELATED 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46284">
        <property type="entry name" value="PROTEIN KINESIN LIGHT CHAIN-RELATED 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13374">
        <property type="entry name" value="TPR_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="349"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7731162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="631" mass="70104" checksum="222B80A8431634EF" modified="2024-05-29" version="1">MSKSGNYSLRHKHLFIVTPPRSPLSTRELDNNIIDLDQASPLAANSYQQLYHNSQTLNRSNTSSSQKYKLNTMQPSTCSNTKTSTHNKINQFSHVKTVSFPITRTKSLHDNNTQTYPRVTRNSTLKVSNAREEPAYLGPYLLKQTREMLSLGENPKKALEVGIRAMKAFESSRVQKPNLEYVMCLHIVAALYCSLGQYAEAIPLLERSIEIPNMGAGLKNSLAKFAGCMQLGDTYAMLGYIENSILYYKAGLGIQRQVLGETDPRYGETCRYVAEAHVRALEFDEAKKLCQMALDIHKVNGSTASVEEAADRRLMGLVCDAKGEYEAALEHYVLASIIMSAASQDSDVAAIDVCIGDAYLSLARYDEAVYTYQKALNVFKATRGDNHPSVASVFVRLAELHYKMGKFRESKSYCINALLIYENPMQGSPNDDIGNGFIEVAGIYESMNEFGAAITLLKKALKILGQDEGQLSTLAGVEAQMGVLYYMMGSYAESYDYLKAAVSKLRVIAEKKSALFGIVLNQLGLACVQMDLFDEAADVFEEAKGVLEVEYGSHHPNTVAVNSNLAGTYDAMGRWEDASEILEYVVRMRKEKLGTASSEVDDERTRLRQLLKDAGRDLSNKDMSLKFLLHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BZ37</accession>
    <name>A0AAD5BZ37_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PNPLA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_010095</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010253">
        <property type="protein sequence ID" value="KAI7732361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BZ37"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050301">
        <property type="entry name" value="NTE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021771">
        <property type="entry name" value="Triacylglycerol_lipase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14226">
        <property type="entry name" value="NEUROPATHY TARGET ESTERASE/SWISS CHEESE D.MELANOGASTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14226:SF10">
        <property type="entry name" value="TRIACYLGLYCEROL LIPASE 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11815">
        <property type="entry name" value="DUF3336"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 4" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="3 4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="74"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="PNPLA" evidence="6">
        <location>
            <begin position="236"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="4">
        <location>
            <begin position="267"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="269"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7732361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="484" mass="55625" checksum="321F5A8020AFDB08" modified="2024-05-29" version="1">MDISSEAAIDRFLIGPSTILGRTIAFRVLFCKSVDQLRHQIVHMLLYYFHRLRTYVSVIGTPVMSWLHPRNPQGILLLVTLIAFLLNRYTNVKTKAKMAYRRKFWRNMMRAAVTYEEWAHAAKMLDKENPKLNESDLYDEELVRNKLQELRHRREECSLRDIIFCMRADLIRNLGNMCNPELHKGRHQVPKLIKEYIDEVSMQLRIVCDFDSDELVLEEKLAFMHETRHAFGRTALLLSGGASLGAFHVGVVKTLVEHKLLPRIVAGSSVGSIMCSVVATKSWPELQSFFEDSWHSLQFFDQMGGIFNVFKRVMTQGALHDIRHLQVLLRNLTNNLTFQEAYDMTGRVLGITVCSPRKHEPPRCLNYLTSPHVVIWSAVTASCAFPGLYEAQELMAKDRSGEIVPYHPPFHWGPEEASGTSGRRWRDGSLEIDLPMIQLKELFNVNHFIVSQANPHIAPLLRLKEFIRAYGGNFAAKVYFLLST</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BZS9</accession>
    <name>A0AAD5BZS9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">60S ribosomal protein L3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_028720</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010161">
        <property type="protein sequence ID" value="KAI7732783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BZS9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000079">
        <property type="entry name" value="60S ribosomal protein L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1430.10:FF:000001">
        <property type="entry name" value="60S ribosomal protein L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.960.10:FF:000001">
        <property type="entry name" value="60S ribosomal protein L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.960.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000351">
        <property type="entry name" value="Ribosomal protein L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1430.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.960.10">
        <property type="entry name" value="Ribosomal protein L3, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045077">
        <property type="entry name" value="L3_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044892">
        <property type="entry name" value="Ribosomal_L3_dom_3_arc_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000597">
        <property type="entry name" value="Ribosomal_uL3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019926">
        <property type="entry name" value="Ribosomal_uL3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11363">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11363:SF5">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00297">
        <property type="entry name" value="Ribosomal_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00474">
        <property type="entry name" value="RIBOSOMAL_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="18"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7732783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="389" mass="44438" checksum="FFD43ADD6E44EEE3" modified="2024-05-29" version="1">MSHRKFEHPRHGSLGFLPRKRAARHRGKVKAFPKDDPTKPCKLTAFLGYKAGMTHIVREVEKPGSKLHKKETCEAVTIIETPPMVVVGVVAYVKTPRGLRSLNTVWAQHLSEDVKRRFYKNWCKSKKKAFIKYSKKFESDEGKKDIQSQLEKMKKYATVIRVLAHTQIRKMKGLKQKKAHLMEIQVNGGTIAQKVDFAYGFFEKQIPIDAVFQKDEMIDIIGVTKGKGYEGVVTRWGVTRLPRKTHRGLRKVACIGAWHPARVSFTVARAGQNGYHHRTEMNKKIYKLGKTGQESHTALTEFDRTEKDITPMGGFPHYGVVKDDYLLIKGCCVGPKKRVVTLRQSLLAQTSRLALEEIKLKFIDTSSKFGHGRFQTAEEKLKFYNRTKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C007</accession>
    <name>A0AAD5C007_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_032846</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase A1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010198">
        <property type="protein sequence ID" value="KAI7732622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C007"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05471">
        <property type="entry name" value="pepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034164">
        <property type="entry name" value="Pepsin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032799">
        <property type="entry name" value="TAXi_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032861">
        <property type="entry name" value="TAXi_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13683:SF743">
        <property type="entry name" value="ASPARTIC PROTEINASE-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13683">
        <property type="entry name" value="ASPARTYL PROTEASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14541">
        <property type="entry name" value="TAXi_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14543">
        <property type="entry name" value="TAXi_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase A1" evidence="3">
        <location>
            <begin position="136"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="154"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7732622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="443" mass="48082" checksum="CABDA4B4BB2A669C" modified="2024-05-29" version="1">MIFPVFNVSCWCWCAFPSPFSFTFSFCHSLAHSPPLYCSSSKSISTVDTNPRSHLFHSMEAARSLLLAVSVTAIVLLMECGLSVQSELMFSSRMIHRQLMDSGERQKMNVGSQHQLLFPSAGTKTLSGGNDLGWLHYAYIAVGVPSVSFLVALDTGSDLLWLPCDCVQCAPLSDNDENEYNPSHSSTSKSITCSHELCESSSTCKNPKQPCPYTVNYASTSTSSSGSLVEDILHLASDGVNGSNTYVRAPVIIGCGKKQSGGYLEGIAPDGVLGLGLSEVSVPSFLAKSGVTRNTFSLCFGESGSGVTYIIGVENFCIGTNCLDRTSFKAQFDSGTSFTFLPDGIYKAVVEEFDRQANATKKTFEDVPFDHCYKSSSQELPETPSVTIKLSVNNTFLVRDPLFIVNDSQALEIIQQPTGYHFIISKTLVLISRDDNECITSIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C1I4</accession>
    <name>A0AAD5C1I4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_010045</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the mTERF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010260">
        <property type="protein sequence ID" value="KAI7732306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C1I4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.70.10">
        <property type="entry name" value="Transcription termination factor 3, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003690">
        <property type="entry name" value="MTERF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038538">
        <property type="entry name" value="MTERF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13068">
        <property type="entry name" value="CGI-12 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13068:SF113">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR MTEF18, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02536">
        <property type="entry name" value="mTERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0806">Transcription termination</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="524"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7732306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="554" mass="64123" checksum="326F654E0C02CAB9" modified="2024-05-29" version="1" fragment="single">MLLRRTLRSLYPHATVRHGSSVAKPLSKIPYKHRAKAIQQAQEALTDYLHSVKTVPYTFAENISRYSIVSLSTVVSKVTFSASDFSKSLQKFFRYHPVNEFELFFESIGIDVNELDLVLPTGKFFLSEDRNVFNVACVLYEFGIPWNKLGILYKEEKTIFYKDPNELREIWKKYIEYGFTSSSLVGICLVFPRVLNGDSEVEAMVNDLKRVVVDFNLVNDVDGDVDTWIGLCRKIRLFYNLGCKKDGISEMIGRSKTILLEYSEEVFCVVGLLRHFGMDEQRLDIITEKYPYVFGKNRLANLPHVMRALRVNQWFFNNLKDGGLHLLKSYAVINSDEDHDKDFAESLVRIQSSRVTAHTLSKLQFLHSIGFGENGLSPKILNQHTKNLEKKVEFLCKEIEFSLDHLDVFPAYLCFDLEKRIRPRYRFHKWLMETGLCEKEYSLASIIATSERSFIARIYKIHPAAPKKYLEIFLSQDYDGCVEHILTSKFQTEMKQRHKGVQCDENVHPDRISSMLLESGGYNGVVYIGGVWVVVEHIFLLLLLFNLKMSNEDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C360</accession>
    <name>A0AAD5C360_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_009716</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the STXBP/unc-18/SEC1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010058">
        <property type="protein sequence ID" value="KAI7733249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C360"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043154">
        <property type="entry name" value="Sec-1-like_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001619">
        <property type="entry name" value="Sec1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027482">
        <property type="entry name" value="Sec1-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036045">
        <property type="entry name" value="Sec1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11679">
        <property type="entry name" value="VESICLE PROTEIN SORTING-ASSOCIATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00995">
        <property type="entry name" value="Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56815">
        <property type="entry name" value="Sec1/munc18-like (SM) proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7733249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="162" mass="18355" checksum="625BAAEECF6CF53A" modified="2024-05-29" version="1" fragment="single">MLLSDMAGNSSLYKDAYIFFSSSVPKELIAELKKEPTIKPRLRAVKEMNLEYFAIDSQCFITDHGKALEELYGDEEISRNGNECLTAMANRIATVFASMLEFPFVRYRAAKSLDPTTMTTFCDLIPTKLAAAVWNCLMKYKTVPNFPQIETCDLLILDRSID</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C4R1</accession>
    <name>A0AAD5C4R1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_002733</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009630">
        <property type="protein sequence ID" value="KAI7734879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C4R1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7734879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="74" mass="8173" checksum="3785FA7E36DE9147" modified="2024-05-29" version="1" fragment="single">MKNGQKPDNRICFVNPAIISPTDERKAKSKHIQDASRAIVDRSLKRKNMDIILLSYNPETIGAMVVYAAQSGTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C637</accession>
    <name>A0AAD5C637_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_007085</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009644">
        <property type="protein sequence ID" value="KAI7734779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C637"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003830">
        <property type="term" value="F:beta-1,4-mannosylglycoprotein 4-beta-N-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006044">
        <property type="term" value="P:N-acetylglucosamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006813">
        <property type="entry name" value="Glyco_trans_17"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12224">
        <property type="entry name" value="BETA-1,4-MANNOSYL-GLYCOPROTEIN BETA-1,4-N-ACETYLGLUCOSAMINYL-TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12224:SF22">
        <property type="entry name" value="GLYCOSYL TRANSFERASE, FAMILY 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04724">
        <property type="entry name" value="Glyco_transf_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7734779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="384" mass="44970" checksum="E5BAE42CF0704661" modified="2024-05-29" version="1">MPDASYSSSKKKDGFCDDVCGQEVSKTARIRCMMQTLDIRTFVVLFVVIPIGFMCVYFHGQKITYFLRPLWQSPPKPFITIPHYYHENVSMDSLCKLHGWKLRDYPRKVYDAVLFNNEIDMLTIRWKELHPYITQFVLLETNSTFTSIPKPHYFAINRERFDFVEPRLTYGTIGGRFKKGENPFVEEAYQRVALDQLLKIAGIEDGDLLIMSDVDEIPSAHTIDLLRWCDGPPPIVHLNLNNYLYSFEFNVDHSSWRASVHRYQKGKTRYAHYRQTDYLLADSGWHCSFCFRKIADFVFKMKAYSHTDRVRFSHFLDPKRIQNVICNGYDLYDMLPEEYTFKDIIAKMGPIPHSYSAVHLPSYLLNNADKYSYLLPGNCIREAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C6K5</accession>
    <name>A0AAD5C6K5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">tRNA(His) guanylyltransferase</fullName>
            <ecNumber evidence="5">2.7.7.79</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">M8C21_011077</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">AA19_3_7</strain>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Adds a GMP to the 5'-end of tRNA(His) after transcription and RNase P cleavage.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a 5'-end ribonucleotide-tRNA(His) + GTP + ATP + H2O = a 5'-end phospho-guanosine-ribonucleotide-tRNA(His) + AMP + 2 diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54564"/>
            <dbReference type="Rhea" id="RHEA-COMP:14193"/>
            <dbReference type="Rhea" id="RHEA-COMP:14917"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:138282"/>
            <dbReference type="ChEBI" id="CHEBI:141847"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.7.79"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the tRNA(His) guanylyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.79" evidence="5"/>
    <dbReference type="EMBL" id="JAMZMK010009387">
        <property type="protein sequence ID" value="KAI7736042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C6K5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008193">
        <property type="term" value="F:tRNA guanylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.3000:FF:000002">
        <property type="entry name" value="tRNA(His) guanylyltransferase 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3000">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025845">
        <property type="entry name" value="Thg1_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024956">
        <property type="entry name" value="tRNAHis_GuaTrfase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007537">
        <property type="entry name" value="tRNAHis_GuaTrfase_Thg1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038469">
        <property type="entry name" value="tRNAHis_GuaTrfase_Thg1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12729">
        <property type="entry name" value="TRNA(HIS) GUANYLYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12729:SF6">
        <property type="entry name" value="TRNA(HIS) GUANYLYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04446">
        <property type="entry name" value="Thg1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14413">
        <property type="entry name" value="Thg1C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNAHis guanylyltransferase catalytic" evidence="15">
        <location>
            <begin position="6"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="Thg1 C-terminal" evidence="16">
        <location>
            <begin position="139"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="tRNAHis guanylyltransferase catalytic" evidence="15">
        <location>
            <begin position="273"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="Thg1 C-terminal" evidence="16">
        <location>
            <begin position="405"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF04446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF14413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAI7736042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="619" mass="72044" checksum="7F395B5FBC8C1ED5" modified="2024-05-29" version="1">MANSKYEYVKSFETEDEVMYPNLIVLRIDGRNFARFSEANEFEKPNDERALNLMNCCATSVLEKYPDIILAYGFSDEYSFIFKKETKFYQRRASKILSLIVSFFTSLYVAKWREFFPREEIRFTPSFKSRIICCPSVEVLQAYLAWRQRECHSKNQYNTCFWMLVKSGKSEIEAHDILKGTQKQEKNEILFQQFSINYKNLPSMYRQGSCILKTEVDENVKIPDNESPVKRLRKMVKIVHSENIASRSFWNDYSYLCKELGCFEQNVNNIKHEYLKLFQSENKLLPFTWIVIRIDGSHFHRFSDVQGFEKPNDELALKLMNASAVAVLEEFNDIVFAYGVSDEYSFILKKNTELYQRRASEIVSAVVSFFSSTYVIKWKEFFPKKELKYPPNFDGRAVCYPSYEIIRDYLAWRQVDCHINNQYNTCFWMLVKSGKSTREAQSLLKGTQAQEKNQMLSQLFGIDYNTLPVIFRNGSSVFWDKVISLSSSGQEMMVTDNGVVAKSHKKVVVEHPNIIEDDFWGAHPLILEVMAMQTKEGDLRFLLQHEDKQLGKEYIMNFSLDSRISVASPLPNMSLTYYVNALSHHVCGGVSTLSFLTMKLTITTTPAFHPDLTGRAPWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C6W6</accession>
    <name>A0AAD5C6W6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">glucan endo-1,3-beta-D-glucosidase</fullName>
            <ecNumber evidence="4">3.2.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_014148</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of (1-&gt;3)-beta-D-glucosidic linkages in (1-&gt;3)-beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.39"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <topology evidence="2">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the glycosyl hydrolase 17 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.39" evidence="4"/>
    <dbReference type="EMBL" id="JAMZMK010009431">
        <property type="protein sequence ID" value="KAI7735845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C6W6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042973">
        <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000002">
        <property type="entry name" value="Glucan endo-1,3-beta-glucosidase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1040:FF:000003">
        <property type="entry name" value="glucan endo-1,3-beta-glucosidase 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000490">
        <property type="entry name" value="Glyco_hydro_17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044965">
        <property type="entry name" value="Glyco_hydro_17_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012946">
        <property type="entry name" value="X8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32227">
        <property type="entry name" value="GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00332">
        <property type="entry name" value="Glyco_hydro_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07983">
        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00768">
        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00587">
        <property type="entry name" value="GLYCOSYL_HYDROL_F17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 12" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="7 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0611">Plant defense</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="domain" description="X8" evidence="13">
        <location>
            <begin position="354"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7735845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="438" mass="48257" checksum="87B051C36EFF61C4" modified="2024-05-29" version="1" fragment="single">VVEGNIGVNYGTVADNLPPPSQVARFLLDNTIINRVKIYDANQEILQAFAHTGIEVTVAVPNNLIPHLAHLKYSQQWVKTNIAPNVPATNIVRILVGNEVLSTTDHSIASHLVLAMQTLHVALVGLDLDRRIKVSTPHSLGILASSSPPSSGRFKQENVALIKQLLGFLQATGSPFMINPYPFFGYSGDTLDYAMFRPNSGVFDDMSNQKYTNMLDAQLDATYSAMKQLGFEDIEIVIAETGWPSKGDPGQAHVDTESAREFNGNLIKHVMSGLGTPLMPNKTFETYIFALFNENLKPGPTSERNFGLFKPDMTPVYHVDILRHNVESTSPSNWNPDRGVPVRSPVPKPNGGKTWCIPKIGAIEKILQANIDYVCGLGFDCQPIREGGRCFYPNSVHAHATYAMNVYYQATGRHVYDCDFRGTGTITPSNPSYGRCKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C7A7</accession>
    <name>A0AAD5C7A7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_025721</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009232">
        <property type="protein sequence ID" value="KAI7736636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C7A7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1310.10">
        <property type="entry name" value="Cullin Repeats"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016159">
        <property type="entry name" value="Cullin_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74788">
        <property type="entry name" value="Cullin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7736636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="66" mass="7803" checksum="A365FC483FBCDB25" modified="2024-05-29" version="1">MGSQEYGDKTWKILEHAIHEIYHNNAIHTLSLEELYRRIKQEMDGTQQSITNDRRHINVHGQNICS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C9U6</accession>
    <name>A0AAD5C9U6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glycosyl transferase family 1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_031401</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009062">
        <property type="protein sequence ID" value="KAI7737303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C9U6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03801">
        <property type="entry name" value="GT4_PimA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000169">
        <property type="entry name" value="UDP-Glycosyltransferase superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041693">
        <property type="entry name" value="Glyco_trans_4_5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47252:SF5">
        <property type="entry name" value="GLYCOSYL TRANSFERASE FAMILY 1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47252">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16994">
        <property type="entry name" value="Glyco_trans_4_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041921938" description="Glycosyl transferase family 1 domain-containing protein" evidence="2">
        <location>
            <begin position="34"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="3">
        <location>
            <begin position="264"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7737303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="475" mass="52933" checksum="32CE9DE2FAF50E98" modified="2024-05-29" version="1">MAKPSTGLSTFFFHKKRWLLLLLAMLSVSTAVAFIIRAAYDSSCDHRQHFNDVPGVGNRIQKTKDTQIGVTSSPLSFMKSKLVLLVSHELSLSGGPLLLMELAFLLRGVGAEVCWITNQKPSGTDEVIYNLENKMLDRGVQVFSAKGQEAINTALKADLVILNTAVAGKWLDAVLTKNVPKVLPKVLWWIHEMRGHYFKLDYVKHLPYVAGSMIDSHVTAEYWENRTQERLKIKMPKTYVVHLGNSKELMDVAEDNVAKRVLREHVRESLGVRNDDILFAAINSVSRGKGQDLFLKSFYESLLLIKEKKLQVPSIHAVIVGSDMSVQTKFETELRDFVAQKKIQHRVHFVNKTLTVAPYLAAIDVLVQNSQGRGECFGRITIEAMAFQLPVLGTSAGGTAEIVVNGSTGFLHPAGKEGVTPLATNIVKLATHVEKRLTMGKRGYERVKQMFLERHMADRIAAVLKDVLHAANARQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CAW1</accession>
    <name>A0AAD5CAW1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidase S54 rhomboid domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_012969</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S54 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008835">
        <property type="protein sequence ID" value="KAI7738307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CAW1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031969">
        <property type="term" value="C:chloroplast membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1540.10:FF:000015">
        <property type="entry name" value="RHOMBOID-like protein 10 chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1540.10">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022764">
        <property type="entry name" value="Peptidase_S54_rhomboid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050925">
        <property type="entry name" value="Rhomboid_protease_S54"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43731">
        <property type="entry name" value="RHOMBOID PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43731:SF26">
        <property type="entry name" value="RHOMBOID-LIKE PROTEIN 10, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01694">
        <property type="entry name" value="Rhomboid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="128"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="166"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="205"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="229"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S54 rhomboid" evidence="7">
        <location>
            <begin position="163"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7738307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="333" mass="36428" checksum="ED6A1F3BE6AAE10A" modified="2024-05-29" version="1">MVGSFAPQPPEFSVSGHKPFHLITNAAALRLGHFLRHSFATHRHHLRSIIHSPIPHLSHVLRLNGLPFEGSQQFEWFKLGQLSHDAWATTFSRCLLFFSGEENKTNYGDQGKAYLRAPTRGPVNNRRLTNMLLAVNVLVYVAQIASQGQVLMWGAKINSLIDKGQLWRLVTSAFLHANVGHLMINCYSLNSIGPTMEHLSGPRRYLAIYMASAVTSSAMSYWFSKAPAVGASGAIFGLVGSFAIFVLRHNKMVKGGVGDLNHVARVIALNMAIGLLSQGIDNWGHLGGLIGGIATSWLIGPAWELKSTTRLGRKVLVDKAPIFSLPGTKRASH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CBF3</accession>
    <name>A0AAD5CBF3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PGG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_008422</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008822">
        <property type="protein sequence ID" value="KAI7738359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CBF3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026961">
        <property type="entry name" value="PGG_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24177">
        <property type="entry name" value="CASKIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24177:SF472">
        <property type="entry name" value="PGG DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13962">
        <property type="entry name" value="PGG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="672"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="714"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="759"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="786"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="domain" description="PGG" evidence="4">
        <location>
            <begin position="662"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="441"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="384"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="441"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7738359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="839" mass="95192" checksum="17B423A71D963DE7" modified="2024-05-29" version="1">MAEPGTELTEVLVDNHEPQDQDTITNDTQMMTKQPGVQLASIDELNPLSQQQQQQININPVAFQANSGPPPPPKLPRSDLFKGSREQYLKIGVPLYEASIKCNWKAAEAIFVKYPQMELERCSITENGETALHVAASAKGSNHVEEFVKKLVDKMQRGDLELQNNNHNTAFYLAAAAGNIKAVKVMLERNRTLLAIAGSNGTMMPLYTAALFGNEDVVKYLYNNSKKLRDDGWTPKNRCWLLQKCVENDMFDVALEIVKTYPELITGDVLGVLARKPEAFREPNINIISRTINWGKDLYSKMLIANPEVDQELSGTPTPFPEVKSDIIKRIIKPAPEKKNALPLLKFIWEDIAKRPKNEIDKILRGSPDSIRQDTTTVSGWAIAMQLQNLISEHVAKMKEETENIITMHPSSTNQDTVKVEQARKLQKLISERLVNLQAETQKITKGPNSMKQDNKSSSSKKAQDLELQNIILDYIANTYDKTQKIVKTIRRIDQAPLLQNFIFDQIEKLDKASQKISIELKPAKETYSSRVLFIAAEMGNTIFLVELIRKYPDLIWKVNDDNQTIFHIAIKHRHEGAMKDLITPLKDLKNNNMLHLVGKIAKQKRLEDVSGVALQMQRELLWFKEVKNMIPPSYRERVNEDGLTPHELFTKEHKDLVTHGEKWMKGTANQCMIVAALIATIVFAAAFTVPGGYDQSNDKTNGIPVFHSKATFMVFVVADAISLFLSCASILIFLSILTSRYAEDDFLDSVPKKLISGLLTLFMSITTMTIAFGVSFFVLYHKGLLWMPILICAFGLLPVLLYIWLQYRLFADVIRSTYRSRYLFKPHKHVLYYETPKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CDX3</accession>
    <name>A0AAD5CDX3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_001281</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008583">
        <property type="protein sequence ID" value="KAI7739664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CDX3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45000">
        <property type="entry name" value="CHAPERONE DNAJ-DOMAIN SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45000:SF5">
        <property type="entry name" value="CHAPERONE DNAJ-DOMAIN SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="2">
        <location>
            <begin position="159"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7739664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="224" mass="26642" checksum="3DEFF698AC15AD75" modified="2024-05-29" version="1">MSEEEEGRSNSRKEEEEIGIWGILIVGLIGATATTLAVGHMRQMTDWFSSKVTRSHSWKAGGSTFQQESWKRYNRRMQEAYEEEMERVERIRRMQSVFNREKSKQKRSYESWKENNAGTGRYHQHFQRDDWYWKTEASQGHRSNFKEPPRSPVNYSLSHHYAILGLSRSRTKPYTDDEIKTAFRSKAKQFHPDQNQDNKEVAEARFKEVMTSYEAIKTERKNMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CE29</accession>
    <name>A0AAD5CE29_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 12">Pectinesterase</fullName>
            <ecNumber evidence="5 12">3.1.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_024272</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Acts in the modification of cell walls via demethylesterification of cell wall pectin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 12">
            <text>[(1-&gt;4)-alpha-D-galacturonosyl methyl ester](n) + n H2O = [(1-&gt;4)-alpha-D-galacturonosyl](n) + n methanol + n H(+)</text>
            <dbReference type="Rhea" id="RHEA:22380"/>
            <dbReference type="Rhea" id="RHEA-COMP:14570"/>
            <dbReference type="Rhea" id="RHEA-COMP:14573"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17790"/>
            <dbReference type="ChEBI" id="CHEBI:140522"/>
            <dbReference type="ChEBI" id="CHEBI:140523"/>
            <dbReference type="EC" id="3.1.1.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Secreted</location>
            <location evidence="1 12">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the C-terminal section; belongs to the pectinesterase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the PMEI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.11" evidence="5 12"/>
    <dbReference type="EMBL" id="JAMZMK010008565">
        <property type="protein sequence ID" value="KAI7739808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CE29"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004857">
        <property type="term" value="F:enzyme inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030599">
        <property type="term" value="F:pectinesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042545">
        <property type="term" value="P:cell wall modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045490">
        <property type="term" value="P:pectin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.10:FF:000001">
        <property type="entry name" value="Pectinesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.40">
        <property type="entry name" value="Invertase/pectin methylesterase inhibitor family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035513">
        <property type="entry name" value="Invertase/methylesterase_inhib"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033131">
        <property type="entry name" value="Pectinesterase_Asp_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000070">
        <property type="entry name" value="Pectinesterase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006501">
        <property type="entry name" value="Pectinesterase_inhib_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018040">
        <property type="entry name" value="Pectinesterase_Tyr_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31707">
        <property type="entry name" value="PECTINESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01095">
        <property type="entry name" value="Pectinesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04043">
        <property type="entry name" value="PMEI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00856">
        <property type="entry name" value="PMEI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101148">
        <property type="entry name" value="Plant invertase/pectin methylesterase inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00800">
        <property type="entry name" value="PECTINESTERASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00503">
        <property type="entry name" value="PECTINESTERASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0063">Aspartyl esterase</keyword>
    <keyword evidence="6 12" id="KW-0134">Cell wall</keyword>
    <keyword evidence="9 12" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="7 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0964">Secreted</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041777220" description="Pectinesterase" evidence="12">
        <location>
            <begin position="18"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="domain" description="Pectinesterase inhibitor" evidence="13">
        <location>
            <begin position="2"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="309"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7739808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="468" mass="52328" checksum="6CFEF1173B114928" modified="2024-05-29" version="1" fragment="single">IIILIFLTSIICSEVLSCDQPPFIELCNLIIGHEFESHESGIKAINETREALERFLAMPHTMFDVQAKTSLIDCLELYGDVINHLNLSKRHTNVADVKRWLKTTIVNHQTCLNGFKYSNFSKLFKNMLVEHKVPASNSPTIHNHVFIRLSKGEAIQANFVVAQDGSGGFSTITNAIEAAENQRIGTDRVVIYVKKGIYKENIIIKKSMTNLLLYGDGIDATVITNDRNIYDGVLTSNTATVQVWGHGFIAIGITFENTAGPEKQQAIALLSASDLSIFYKCSFKGYQDTLCILENRQFYRECDIYGTVDFIFGDASAVLQNCNIYVRTPLQGQQNTITAQGRYHSSSNTGFVIENSRVSPAPDSNLADGLVMTYLGRPWRDYSRVVYVKCDFDNVINPAGWLPFQGDSAFNKVYYAEYMNTGQSADTSGRITWPGYHVLTTDEEAEQFSVRIFLTGESWIPKSGVPFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CEK7</accession>
    <name>A0AAD5CEK7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_022620</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008603">
        <property type="protein sequence ID" value="KAI7739595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CEK7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049198">
        <property type="entry name" value="DUF6865"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21737">
        <property type="entry name" value="DUF6865"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7739595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="141" mass="15806" checksum="A93B89007C1E9E30" modified="2024-05-29" version="1" fragment="single">LKGIENYTLERIVWVQKIQTKDCLQQTFSFPCPCFPSNTDEPTEPEIDAVGSPIIAISQGKNKHYMDYIPGLEDVSLENVRESLIAISYCEPERVSCVTEHEVLNHENNNVVVVSEETAIIDESRSKLMSIAASPTMDCND</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CGB9</accession>
    <name>A0AAD5CGB9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_006484</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Organophosphate:Pi antiporter (OPA) (TC 2.A.1.4) family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the major facilitator superfamily. Phosphate:H(+) symporter (TC 2.A.1.9) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008277">
        <property type="protein sequence ID" value="KAI7741082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CGB9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055062">
        <property type="term" value="P:phosphate ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000050">
        <property type="entry name" value="glucose-6-phosphate exchanger SLC37A2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000028">
        <property type="entry name" value="Sugar phosphate exchanger 3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000849">
        <property type="entry name" value="Sugar_P_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43184:SF15">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE TRANSPORTER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43184">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY TRANSPORTER 16, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002808">
        <property type="entry name" value="Hexose_phosphate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="130"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="158"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="198"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="223"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="298"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="332"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="367"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="396"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="433"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="464"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="11">
        <location>
            <begin position="44"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7741082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="513" mass="55296" checksum="DB04DCBA11053CE0" modified="2024-05-29" version="1">MGSISEPNEQSNRRNTKRPPGIRFIEHTKNSTISFKSHQAIVLIVTFLAYTSYHASRKTTSIIKSALDPQTPDESEQSNALWWVLGNGWAPFNGANGTVLLGDIDLAFLTVYAVGMFFSGHIGDRMNLRMFLTIGMVGTGLFTALFGVGYWANIHVFYYYLIVQMIAGLFQSTGWPSVVAVVGNWFGKTKRGLIMGVWNAHTSIGNISGSLIAAYFLKYGWGWSMVVPGLMIAVVGVVVFLFLPVDPESVGVVRDEDESGPPKNKEVTEPLLSSNTVQVDTEKGSAVGFFEAWRIPGVAPFAFCLFFAKLVAYTFLYWLPFYISHTAINGEYLSSTAAGNLSTLFDVGGVVGGILAGHISDRLDARAITAASFTYCAIPALYLYKSYGHISMSVNIILMLIIGMFVNGPYALITTAVSADLGTHRSLKGNSKALATVTAIIDGTGSIGAAIGPVLTGYISTHSWSAVFTMLMLSAFVAGLFLTKLVVAEVTAKCQDVQGESSDFTPSSVIEEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CGP7</accession>
    <name>A0AAD5CGP7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">glutathione transferase</fullName>
            <ecNumber evidence="1">2.5.1.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_031156</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="1"/>
    <dbReference type="EMBL" id="JAMZMK010008204">
        <property type="protein sequence ID" value="KAI7741428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03185">
        <property type="entry name" value="GST_C_Tau"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd03058">
        <property type="entry name" value="GST_N_Tau"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000018">
        <property type="entry name" value="Glutathione S-transferase U20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000014">
        <property type="entry name" value="Tau class glutathione S-transferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045074">
        <property type="entry name" value="GST_C_Tau"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045073">
        <property type="entry name" value="Omega/Tau-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11260:SF773">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE U26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11260">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE, GST, SUPERFAMILY, GST DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13410">
        <property type="entry name" value="GST_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01152">
        <property type="entry name" value="Main.3:_Omega-_and_Tau-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="4">
        <location>
            <begin position="22"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="5">
        <location>
            <begin position="107"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="GST N-terminal" evidence="4">
        <location>
            <begin position="267"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00047960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7741428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="453" mass="52659" checksum="5BE484435C0A74AA" modified="2024-05-29" version="1" fragment="single">SLTLHAYMQLTLTTTMGEQQEEEVILLDFWPSMFGMRVKIALAEKGIPYEFREEDTRNKSQLLLDMNPVHKKIPVLIHNGKPICESNIIVQYIDEAWNHKAPLLSSDPYANARARFWADFIDKKVYQAGRNLYTTKGEEHEASRAEFLECLKLLEGELGDKPYFGGETFGYLDVSLIPFYSWFQAYETYGNFNFEHECPKLFAWAKRCIQNKESVSKTLPDLPKLHVTIMSHSSTSFHTHILYNQILTPSSPTTTNQTKTRMGEQQEQLVLLDFWASMFGMRVRIALAEKGIPYEFREEDLRNKSQLLLDMNPIHKKIPVLIHNGKPICESSIIVQYIDEVWNHKAPLLPSDPYAKAHARFWADFIDKKLYQTGRNLYTTKGEEHEASRXXXXVKLLAYETYGNFNFEHECPKLFAWVKRCIQNKESVSKTLPELPKVFAFVQHLRKRFGIDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CH38</accession>
    <name>A0AAD5CH38_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">E3 ubiquitin-protein ligase listerin</fullName>
            <ecNumber evidence="1">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">RING-type E3 ubiquitin transferase listerin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_024418</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">E3 ubiquitin-protein ligase. Component of the ribosome quality control complex (RQC), a ribosome-associated complex that mediates ubiquitination and extraction of incompletely synthesized nascent chains for proteasomal degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the ribosome quality control complex (RQC).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the LTN1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="1"/>
    <dbReference type="EMBL" id="JAMZMK010008256">
        <property type="protein sequence ID" value="KAI7741150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CH38"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990112">
        <property type="term" value="C:RQC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072344">
        <property type="term" value="P:rescue of stalled ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990116">
        <property type="term" value="P:ribosome-associated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039795">
        <property type="entry name" value="LTN1/Rkr1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12389:SF0">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE LISTERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12389">
        <property type="entry name" value="ZINC FINGER PROTEIN 294"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU367090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7741150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="460" mass="51732" checksum="52016113B38F2198" modified="2024-05-29" version="1" fragment="single">DLHAVRKGDSWPLSYLVGPVLSKSFQLIQTIDSSNPVKFMVAAVSTFGPRKFIQEVMLEQNECSSRSEEKNRNLSLKRFLQHFNEIFVPWCLQTDSCSTSARLNLLFALLDDECFSEQCNSIILHAASSHDSYYTCILALVMEKTREECIKKKLHVDLSHHELLGSIALSVTCSFPPFGCSDARAALGGKTEEDPTCLLSENTSTVIYEELFEKLRSFIGTSNFTWVKDANKELSKAKEHLTIKGCESSVSLLEMANFAMEVLNGSFFRLINLSKNSTLLAHVLAALFVIDWEHSTSVVFYEGLDIEAYSEAKDRYNFCKSVHSFRRKINKFLKTISPDCRSTLGSTLVQAIRCVVFNEDKLEIDKVTSLGCLFFLDVMDNLCQSQVEEQTLMDQLLNNSASWPLWVTFDGQRSATLKTEIKFSDASENYRVATFVEKLMLKVGLARVIAGSISSEDSMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CH71</accession>
    <name>A0AAD5CH71_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_023959</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein disulfide isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008140">
        <property type="protein sequence ID" value="KAI7741728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CH71"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003756">
        <property type="term" value="F:protein disulfide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034976">
        <property type="term" value="P:response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18929">
        <property type="entry name" value="PROTEIN DISULFIDE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18929:SF246">
        <property type="entry name" value="PROTEIN DISULFIDE ISOMERASE-LIKE 1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="2">
        <location>
            <begin position="82"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7741728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="146" mass="16548" checksum="0D31701C8271454E" modified="2024-05-29" version="1" fragment="single">VSVFVLIHISNPRSGFLCFCVHRQSASSFGFLIFVFVDRDNEDVGKPVVDYFGITVDAPKVYGEDFLNYKLKPLYKSRSRAEIYAPWCGHYQALEPTYNKLAKHLPKMDGSTNEHPRAKADEYPTILLCPARNKSSDPVDKSDPMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CHQ5</accession>
    <name>A0AAD5CHQ5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_003265</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008133">
        <property type="protein sequence ID" value="KAI7741832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CHQ5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042258245" description="Secreted protein" evidence="1">
        <location>
            <begin position="17"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7741832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="68" mass="7659" checksum="CCE797ACABB9E1DD" modified="2024-05-29" version="1">MIILLCTFVCVCRIHNEGCYNVACLSIFDNIRVSFFGGFKLLGTCCPVFRDDNMVLLEMINQVGTSFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CHS2</accession>
    <name>A0AAD5CHS2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_032187</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008067">
        <property type="protein sequence ID" value="KAI7742142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CHS2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7742142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="69" mass="7391" checksum="60E19F060D70BBDF" modified="2024-05-29" version="1">MAATIRGIIPFLPAAAANKRAVSPSEFSSSKSSSVIKATRFCTNDATLDQLHLWVLIKDGFRSIPIASN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CI70</accession>
    <name>A0AAD5CI70_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_002471</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PYR/PYL/RCAR abscisic acid intracellular receptor family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008033">
        <property type="protein sequence ID" value="KAI7742283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CI70"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010427">
        <property type="term" value="F:abscisic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004864">
        <property type="term" value="F:protein phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009738">
        <property type="term" value="P:abscisic acid-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07821">
        <property type="entry name" value="PYR_PYL_RCAR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.530.20:FF:000039">
        <property type="entry name" value="Bet v I allergen-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050279">
        <property type="entry name" value="Plant_def-hormone_signal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019587">
        <property type="entry name" value="Polyketide_cyclase/dehydratase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31213">
        <property type="entry name" value="OS08G0374000 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31213:SF210">
        <property type="entry name" value="POLYKETIDE CYCLASE_DEHYDRASE, START-LIKE DOMAIN SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10604">
        <property type="entry name" value="Polyketide_cyc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0938">Abscisic acid signaling pathway</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0650">Protein phosphatase inhibitor</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7742283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="172" mass="19234" checksum="DF171B88F6ACF570" modified="2024-05-29" version="1">MDAAHYIRRHHRHNPGHQQCASAVVKHITAPVDIVWSLVRRFDQPQSYKPFVSRCTMRGGLNIGSVREVDVKSGLPATTSTERLELLDDTEHILGIKIVGGDHRLKNYSSILTVHPEVTEGRSGTLVIESFVVDIPNGNTQDETCYFVKALINCNLKSLSDISERMAVKDQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CKP9</accession>
    <name>A0AAD5CKP9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_012014</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008019">
        <property type="protein sequence ID" value="KAI7742324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CKP9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33494:SF1">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33494">
        <property type="entry name" value="OS02G0793800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="143"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7742324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="183" mass="20942" checksum="599AF162871C08B2" modified="2024-05-29" version="1" fragment="single">NNASRILTHPFYRVDSVSDYRLHHQKTDHKSTVKNTRHNFYSVDLKLLMVQLMKNSNNSPELQKHEKMTPVKREITEEHTQYDQQQERINKRPRHQEIRIGSFPVSPAQYNPLDEPSPLGLRLRKSPSLLELIQMKLSEGNAASKLGSDSKRGNKNTTASAATDKLKASNFPASLLKIGTWEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CKZ9</accession>
    <name>A0AAD5CKZ9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_018093</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007655">
        <property type="protein sequence ID" value="KAI7743851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CKZ9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7743851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="81" mass="8997" checksum="B2BAE457DB53E27A" modified="2024-05-29" version="1">MIFTHDDYNWSGVISSFLPNLQALSFKSCNLSGPLDSSLAKLKNLSVIRLDGNTFSSAIPDSYFFKALFPIRLGLLISLRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CL60</accession>
    <name>A0AAD5CL60_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RanBP2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_031030</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007701">
        <property type="protein sequence ID" value="KAI7743662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CL60"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="entry name" value="Histone deacetylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01358">
        <property type="entry name" value="ZF_RANBP2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RanBP2-type" evidence="5">
        <location>
            <begin position="118"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS01358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7743662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="246" mass="27383" checksum="1487CC155570C03C" modified="2024-05-29" version="1" fragment="single">KFIWLSSHDQNICTPKFLKINQAALTPFSAQSGMVVPGGHTDHKSVSSTMQINGLGNGEQENNVHDQIILTNVTAVKKNSRKHEDMTLEDMYGKDGDFDDEDDSDWEPVEKNLTVATWFCVNCTLLNLDDAIYCHICGEHKESGILRHGFFASRSHEADSVMNKSLERNGKSEASCSKSIAPHKLTAVGFDERMLLHSEVEMKSHPHPERPDRLRAIAASLATAGIFPGKCHPISAREITREELLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CL70</accession>
    <name>A0AAD5CL70_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">60S ribosomal protein L10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_006286</name>
        <name evidence="5" type="ORF">M8C21_011947</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012211">
        <property type="protein sequence ID" value="KAI7724477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAMZMK010007955">
        <property type="protein sequence ID" value="KAI7742609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CL70"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010224">
        <property type="term" value="P:response to UV-B"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01433">
        <property type="entry name" value="Ribosomal_L16_L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1170.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.10">
        <property type="entry name" value="Ribosomal protein L10e/L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047873">
        <property type="entry name" value="Ribosomal_uL16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018255">
        <property type="entry name" value="Ribosomal_uL16_CS_euk_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016180">
        <property type="entry name" value="Ribosomal_uL16_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001197">
        <property type="entry name" value="Ribosomal_uL16_euk_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036920">
        <property type="entry name" value="Ribosomal_uL16_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003239">
        <property type="entry name" value="PRK04199.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00279">
        <property type="entry name" value="uL16_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11726">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00252">
        <property type="entry name" value="Ribosomal_L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005590">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54686">
        <property type="entry name" value="Ribosomal protein L16p/L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01257">
        <property type="entry name" value="RIBOSOMAL_L10E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7724477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7742609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="220" mass="24951" checksum="60758DEB5F4849A6" modified="2024-05-29" version="1">MGRRPARCYRQIKNKPYPKSRYCRGVPDPKIRIYDVGMKKKGVDEFPFCVHLVSWEKENVSSEALEAARIACNKYMTKFAGKDAFHLRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGVCARVSIGQVLLSVRCKDGNSQHAQEALRRAKFKFPGRQKIIVSRKWGFTKFSRTDYLQWKSENRIVSDGVNAKLLGCHGPLANRQPGRAFLDAVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CL76</accession>
    <name>A0AAD5CL76_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_033148</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007959">
        <property type="protein sequence ID" value="KAI7742551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CL76"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7742551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="69" mass="7649" checksum="0C030B885480DB97" modified="2024-05-29" version="1" fragment="single">MFQIADILGELKGIEFSLDPLEKQIGDEIIGLLQEGQNLNNCENNELETFQQAATKLGITSSRGAVRER</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CR95</accession>
    <name>A0AAD5CR95_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_034022</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010007371">
        <property type="protein sequence ID" value="KAI7745056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CR95"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000258">
        <property type="entry name" value="Probable serine/threonine-protein kinase PBL8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000228">
        <property type="entry name" value="Serine/threonine-protein kinase BIK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050823">
        <property type="entry name" value="Plant_Ser_Thr_Prot_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45621">
        <property type="entry name" value="OS01G0588500 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0611">Plant defense</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="94"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="374"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="11"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="49"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="385"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7745056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="434" mass="47886" checksum="CB1F3856BE537C07" modified="2024-05-29" version="1">MGNCGTREESATASDANNNNNEGTVSDLGNQLQVTSGKSQGLEKKRSRSISGLSEPSTPRNDMNISEALKNNSMSYNSITSFTMFELETVTKSFRSDYILGGGGFGTVYKGYIDENVRVGLKSLPVAVKVLNKEGRQGHREWLTEVSLLGQLRHPNLVKLIGYCCEDDNRLLVYEFMFRGSLENHLFRKASVPLSWSKRMMIAYGAAKGLAFLHNSEWPVIYRDFKTSNILLDSDYTAKLSDFGLAKAGPRGDETHVSTRVMGTYGYAAPEYVMTGHLTVRSDVYSFGVVLLELLTGRKSVDKTRPNKEQSLVDWARPKLKDKKKMLLIIDPRLQDRYSLRAAQKACSLAYYCLSQNPKARPLMTDVVETLEPLQHGGSNDATMSSSVSPLASSGGNSDNRMGHRFVRTVGSAGGCRSRNVNCAQGTPAPCRVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CSM2</accession>
    <name>A0AAD5CSM2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Branched-chain-amino-acid aminotransferase</fullName>
            <ecNumber evidence="9">2.6.1.42</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_010287</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-isoleucine + 2-oxoglutarate = (S)-3-methyl-2-oxopentanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24801"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:35146"/>
            <dbReference type="ChEBI" id="CHEBI:58045"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:18321"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:17865"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-valine + 2-oxoglutarate = 3-methyl-2-oxobutanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24813"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57762"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.42" evidence="9"/>
    <dbReference type="EMBL" id="JAMZMK010006727">
        <property type="protein sequence ID" value="KAI7747573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CSM2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004084">
        <property type="term" value="F:branched-chain-amino-acid transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009082">
        <property type="term" value="P:branched-chain amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01557">
        <property type="entry name" value="BCAT_beta_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.10.10:FF:000003">
        <property type="entry name" value="Branched-chain-amino-acid aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.10:FF:000003">
        <property type="entry name" value="Branched-chain-amino-acid aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.10.10">
        <property type="entry name" value="D-amino Acid Aminotransferase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001544">
        <property type="entry name" value="Aminotrans_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018300">
        <property type="entry name" value="Aminotrans_IV_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036038">
        <property type="entry name" value="Aminotransferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005786">
        <property type="entry name" value="B_amino_transII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043132">
        <property type="entry name" value="BCAT-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043131">
        <property type="entry name" value="BCAT-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033939">
        <property type="entry name" value="BCAT_family"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01123">
        <property type="entry name" value="ilvE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009897">
        <property type="entry name" value="PRK13357.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42825">
        <property type="entry name" value="AMINO ACID AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42825:SF29">
        <property type="entry name" value="BRANCHED-CHAIN-AMINO-ACID AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01063">
        <property type="entry name" value="Aminotran_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006468">
        <property type="entry name" value="BCAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56752">
        <property type="entry name" value="D-aminoacid aminotransferase-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00770">
        <property type="entry name" value="AA_TRANSFER_CLASS_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="3 9" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="9" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="5 8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="6">
        <location>
            <position position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR006468-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7747573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="367" mass="40305" checksum="0A1DCE9A061C45AA" modified="2024-05-29" version="1">MVIFLKVKGRCLTTHVTSTLQETEPMGRYEEYADIDWNNLGYGLIKTDYIYKAKCTLNNTFEQGQVCDYGNIELSPGAGVLNYGQGIFEGTKAFRGEDGRILLFRPDQNAIRMQIGAERMCMQSPSVQQFVDAVKQTAIANKRWIPPPGKGSLYIRPLLIGTGPTLGVSPAPEYTFLVYASPVGNYFKAGTGPLNLYVNNEFHRATRGGAGCVKSITNYAPVLKAITGAKEQGFSDVVFLDSVHKKYIEEASCSNIFLVKGNVISTPATVGTILEGITRKSIIDIARDLGYKVEERLIALDELMEADEVFTTGTAVSVATVGSVTFNAQRIEYKTAGELVSQKLYKTLVGIQVGKIKNKYDWAVEIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CTP8</accession>
    <name>A0AAD5CTP8_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_031379</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006840">
        <property type="protein sequence ID" value="KAI7747120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CTP8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7747120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="86" mass="9832" checksum="B54437EF69462914" modified="2024-05-29" version="1">MGNLKVLILRSYYTRLLCALGNPKALRVSYDKDISTRSEGLALRFLCLGGWYVIDQLLSIPLSSCLKMLKHMQQRSTKEVMCPFFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CUG5</accession>
    <name>A0AAD5CUG5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_012259</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006516">
        <property type="protein sequence ID" value="KAI7748503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CUG5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36617:SF5">
        <property type="entry name" value="OS05G0421675 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36617">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="241"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7748503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="368" mass="41588" checksum="09F329B3B8051B2C" modified="2024-05-29" version="1">MFRRKSSLDMDVYKLHYIAKVVDNGLAEQGAKRLVPVGLGDGDQCMEDDFTAWLLDTAHMEYCLCMYITGCFQQESVISLALQLSCSETQPLKVEMEVENMKVEVGDGSNTRFWKDRWVRSVPLMATFPGLYRITTNKNGSVADHICYRGDVIFWVWSWVRNPVSRGEWTQIGRLMDLLHSKALKNGKDSWVEVGDGSNTRFWKDRRVVSLIMRSEVMFHLHFLAGVEADQSKEVGLTKSSRTKSFEENQGPVNEQPGLAGKQNVQPGLAGKQNAILDFPSGSTFFPGVVSLAKDRSQGLTAMEINGVHINIRKSRLLALVSPLRAELEKQHAELKLKSSEVINFMHSKLDKRTKTYPQTAIVLKWEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CUX8</accession>
    <name>A0AAD5CUX8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RanBP2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_005346</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006683">
        <property type="protein sequence ID" value="KAI7747810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CUX8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1060.10:FF:000014">
        <property type="entry name" value="Putative zinc finger, RanBP2-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1060.10">
        <property type="entry name" value="Zinc finger, RanBP2-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036443">
        <property type="entry name" value="Znf_RanBP2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23111:SF29">
        <property type="entry name" value="OS07G0404300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23111">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00641">
        <property type="entry name" value="Zn_ribbon_RanBP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00547">
        <property type="entry name" value="ZnF_RBZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90209">
        <property type="entry name" value="Ran binding protein zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01358">
        <property type="entry name" value="ZF_RANBP2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50199">
        <property type="entry name" value="ZF_RANBP2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RanBP2-type" evidence="6">
        <location>
            <begin position="343"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="RanBP2-type" evidence="6">
        <location>
            <begin position="376"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="314"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="410"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="463"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="413"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="466"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="500"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="529"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="550"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="564"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="606"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="636"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="689"/>
            <end position="699"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7747810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="734" mass="84114" checksum="972C8EACD9898476" modified="2024-05-29" version="1">MGLSHILRSQSHHLLFPIITHKPIIFTNPFSSNSPEPKPTSLSARMSFVFDQIDAIEKERTQKDQTLERIRAWRQSKKEKQQQPTIQQDVKETDDVVVKEKKGLGNKEVELVHPWPEWIELMERLVHHNYFDCRRRDEGKLIEESGFGDVGDGDSECGGVMDEVMEEGFDFTRDWKTVQTAGLSFGKDRFDIFRSLSRQDIQLLVGYGCPSPDKKVVFSSKLLRKHVHLDEGDVCSSCSLRNSCERAYLLTNKEDEARTIDIMRVLMTYGFDPVNGSVVNDSLLKKKTVKGAVRKVLHEVVKLSGIPIDPNLAPPIIKRPPPKVKQTPPPPKRRVGRDDVEMKKGDWLCPKCDFMNFAKNTVCLQCDAKRPKRQLLPGEWECPECQFLNYRRNMACFHCECKRPPDEFTENQMQDRQHGQRSRVDHGPSRKEVSDAWNFDFDENESDGAEVAAFEYADSRKLDQDFATTRPDNNSFSRRPVRPQESQYSGFVAKPGVGFDDFDDEEDDDVNSYELDTKPSSSSVNFSDIDVDSESEDENYNARRPVSSQRNKRASKSSKKSAVSEDDGVDFNSDDDLPIHPNWKSSHVADFRPKSRGNMRSKLGSDEDSDLDFGGEDLSDNDFSHKRKGRMNTRSNDYHKVKRDFSDEDGHFSRDKPRGRKAVSDRNKHPARSFRNDSYERGSNGFRNHKQESFSERRPRTSNRARGSSGSGSGAVSDSYLDHERFSRPRVNVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CVN0</accession>
    <name>A0AAD5CVN0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Serine aminopeptidase S33 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_024934</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006453">
        <property type="protein sequence ID" value="KAI7748847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CVN0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277">
        <property type="entry name" value="ALPHA/BETA HYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277:SF154">
        <property type="entry name" value="ALPHA_BETA-HYDROLASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine aminopeptidase S33" evidence="2">
        <location>
            <begin position="69"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="265"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7748847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="378" mass="43529" checksum="948747B8F5DB6575" modified="2024-05-29" version="1">MGGVTSSMAAKLAFFPPNPPFYKLITEKSSGLLLLDRFPHRENVDVLKIPTRRGNKIVAVYVRYPMATKTLLYSHGNAADIGQMYELFIDLSIHLKVNLLGYDYSGYGRSTGKPSEHNTYADIEAAYKCLEETYNTKQEDIILYGQSVGSGPTLHLAARLPRLKAVVLHSPILSGLRVMYPVKHTYWFDIYKNIDKIQFVKCPVLVIHGTSDEVVDCSHGKQLWELCEEKYEPLWIKGGKHCNLELFPEYIKHLKKFISAIDKPLSSRRNNNNSRKSVDQSTSTNHHHNSSRRKSTDCFEAPRKSTCKKEPLDFINELEPFALKFDMHDDKVGKLRIPYEHMERSRRSVEYFEKPRSSTDIKPEWPRMSVDCIDRIQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CWL9</accession>
    <name>A0AAD5CWL9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Response regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_022265</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006478">
        <property type="protein sequence ID" value="KAI7748725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CWL9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009736">
        <property type="term" value="P:cytokinin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000007">
        <property type="entry name" value="Two-component response regulator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045279">
        <property type="entry name" value="ARR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006447">
        <property type="entry name" value="Myb_dom_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01557">
        <property type="entry name" value="myb_SHAQKYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43874">
        <property type="entry name" value="TWO-COMPONENT RESPONSE REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43874:SF137">
        <property type="entry name" value="TWO-COMPONENT RESPONSE REGULATOR ARR11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="Response regulatory" evidence="8">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="211"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="223"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="235"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7748725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="437" mass="50058" checksum="1161B67472A0AEF8" modified="2024-05-29" version="1" fragment="single">INGFSSPRADVFPAGLRVLVVDDDPTWLKILEKMLKKCSYEVMSVDGETRRVMRGVQHGACDYLLKPIRMKELRNIWQHVYRKKIHEVRDTECHEGLEEILMMRNVSEQQSDDGYLYCGGDMTPGSKKRKDFEHKYEEKDFADHSSVKKARVVWTVDLHQKFVKAVNLIGFEKVGPKKILDLMNVPWLTRENVASHLQKYRLYLTRLQKDDLKTSSSGPKPDIPPRDAPDKCTESTKVQQNEVVSSHRTPNNLIIQNSSPTIHEVDIKGNASLQTTKPKKILTGYAAKTGMSHPFASTQYSSFNSVLPTQYSWGGFSKEIQFKQENNPPQFQVQDGLFPGLGLPAQQNIMPSFQPPDPVISPSWSLKDHPNNMELSPFLLQGQHFSSTNVDHEYKGVVEYNDQEFLYDPLRYDYEYPTDPLEFSVIDQGLLSRHVII</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CY86</accession>
    <name>A0AAD5CY86_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_016623</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006317">
        <property type="protein sequence ID" value="KAI7749640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CY86"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7749640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="90" mass="10284" checksum="F06D4FA94E17C30C" modified="2024-05-29" version="1" fragment="single">MYQEIGVGVLTGMRVWASLESDGRIMRQKDMKFERVFFKITVSFRLMGNNNNNNNNKNKKKMQQEGGGSQQHSLATVFVSNLPYSFSTSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CYM1</accession>
    <name>A0AAD5CYM1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF7880 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_027500</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006268">
        <property type="protein sequence ID" value="KAI7749835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CYM1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057202">
        <property type="entry name" value="DUF7880"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36014">
        <property type="entry name" value="OS03G0176600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36014:SF1">
        <property type="entry name" value="OS03G0176700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25306">
        <property type="entry name" value="DUF7880"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7880" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF25306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7749835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="57" mass="6211" checksum="3D8CD5F223E8A6A9" modified="2024-05-29" version="1">MKSNIGIAVNALNSLLQTVPTDVLEKGKAIDDAYNTPEEGYPLENLDPILQQLESIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CYY9</accession>
    <name>A0AAD5CYY9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Homeobox protein knotted-1-like 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_023783</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TALE/KNOX homeobox family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006069">
        <property type="protein sequence ID" value="KAI7750873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CYY9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000143">
        <property type="entry name" value="homeobox protein knotted-1-like 3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005539">
        <property type="entry name" value="ELK_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008422">
        <property type="entry name" value="KN_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005540">
        <property type="entry name" value="KNOX1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005541">
        <property type="entry name" value="KNOX2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050224">
        <property type="entry name" value="TALE_homeobox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11850">
        <property type="entry name" value="HOMEOBOX PROTEIN TRANSCRIPTION FACTORS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03789">
        <property type="entry name" value="ELK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05920">
        <property type="entry name" value="Homeobox_KN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03790">
        <property type="entry name" value="KNOX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03791">
        <property type="entry name" value="KNOX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01188">
        <property type="entry name" value="ELK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01255">
        <property type="entry name" value="KNOX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01256">
        <property type="entry name" value="KNOX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51213">
        <property type="entry name" value="ELK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 5" id="KW-0371">Homeobox</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ELK" evidence="9">
        <location>
            <begin position="325"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="8">
        <location>
            <begin position="345"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox; TALE-type" evidence="5">
        <location>
            <begin position="346"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="23"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="404"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="28"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="40"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7750873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="444" mass="50670" checksum="84377E3B2BB29E72" modified="2024-05-29" version="1">MAFNDNLSHEMALQHYENDDVLTGILPSDHHNHHDDHLTHSSSPKPSQSQPPTWLNSAILRQHNHHYSGEPTAASGGPGGSGSFLHLQTTISDSSNTNNHWLSPRPIDNSNENNININHNRETNMFVSGHNNDSNIDENEMKVISQRGNCNGNDERDWELEKCKADILSHPMYDQLLSAHVSCLRIATPVDQLPRIDAQLAQSQQVIDKYSVFGNQNQHMDDKELNQFMAHYVILLSSFKEQLQQHVRVHAMEAVMACWELEQSLQSLTGVSPGEGTGATMSDDDDEQVDGDTTMFDGGLDVSDSMGFGLPTESERSLMERVRQELKHELKQGYKEKIVDIREEILRKRRAGKLPGDTTSLLKAWWQSHSKWPYPTEEDKARLVQETGLQLKQINNWFINQRKRNWHSNPSSSTAAKSKRKRYQNAYTHIQRINLDRNDRMDIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CZX6</accession>
    <name>A0AAD5CZX6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Agenet domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_012365</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006144">
        <property type="protein sequence ID" value="KAI7750517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CZX6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd20405">
        <property type="entry name" value="Tudor_Agenet_AtDUF_rpt1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20406">
        <property type="entry name" value="Tudor_Agenet_AtDUF_rpt2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008395">
        <property type="entry name" value="Agenet-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014002">
        <property type="entry name" value="Agenet_dom_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007930">
        <property type="entry name" value="DUF724"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31917">
        <property type="entry name" value="AGENET DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31917:SF114">
        <property type="entry name" value="AGENET-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05641">
        <property type="entry name" value="Agenet"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05266">
        <property type="entry name" value="DUF724"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00743">
        <property type="entry name" value="Agenet"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0341">Growth regulation</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Agenet" evidence="5">
        <location>
            <begin position="5"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="Agenet" evidence="5">
        <location>
            <begin position="98"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="203"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="409"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7750517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="498" mass="56633" checksum="C86F288EB9F3CC08" modified="2024-05-29" version="1" fragment="single">MANSYSLTKGSTVEVRCEEVGLEGAWYVATILDQLLTTKLHTNHKKKKVSYLIKYHTLFFKQHDHDDDDLLLPLTDVVDPSFIRPSPPPNEDNNNISKEFEVGDVVDAYHRDGWWIGVVKTVIINQPQLNNYIVSFEDQELMFEKSNLRFHVDWVDSRWIIPPKQTLKQLAAGDGIATDNNVLSVAQMTEEALLPSSSAKIKAVSRKRSKSDSAAKTSRAKRIQPSPGGKGNTKVTVSVMETSRSDDIHQSSPPETIITTCELKDGMGHRNRKKGRPPKLVVEKPVGLLQGSSCFSPPDHNGNSLSQASAGTTSDYQQDWPFIKRFPIWDTIELYSQKPHFSPLKTRKEEFREGLAIGYMVTFWNLAQRLSDLQLNDPADELNNILETLCDLEIHGFDVGPIRTRLNDLLTLKSKVSQHEDTRKKVEKELQKCKHEKSQMEKEIDQYKTKTRVLKLKTKRAATLWKRKDGEVTRLRSKRILICNQISDWKLAFGELAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CZZ2</accession>
    <name>A0AAD5CZZ2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_026296</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006189">
        <property type="protein sequence ID" value="KAI7750199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CZZ2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7750199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="48" mass="5330" checksum="106B46002513EE9F" modified="2024-05-29" version="1">MFCRNVFAEDGSSKLDASALPAVMSFTRYIRRECICLSLTGTLHFLAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D0G4</accession>
    <name>A0AAD5D0G4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="2">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_021313</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010005949">
        <property type="protein sequence ID" value="KAI7751244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D0G4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16667">
        <property type="entry name" value="RING-H2_RNF126-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000022">
        <property type="entry name" value="E3 ubiquitin-protein ligase RING1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010543">
        <property type="entry name" value="DUF1117"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039525">
        <property type="entry name" value="RNF126-like_zinc-ribbon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15710">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE PRAJA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15710:SF146">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RING1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06547">
        <property type="entry name" value="DUF1117"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14369">
        <property type="entry name" value="Zn_ribbon_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="10">
        <location>
            <begin position="208"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="70"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="254"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="364"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="260"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="381"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="394"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7751244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="403" mass="43607" checksum="36BC2C8AE28060E3" modified="2024-05-29" version="1">MSTVGSLSSSYWCYRCNRSVRIRTHLQDSLLSCPDCNGGFIEEITTPTRLNGSGSDFYRSRFPAAAMYMGNGNGNGLQSPGPGRSPSPPVLRRGRRNGGDRSPFNPVIVLRGPTSTPEESAEEVGSGAGTGFELFYDDGAGSGLRPLPASMSEFLLGSGFDRLLDQLAQIEGNGLGRIDNNAPASKAAIEAMPTIEIQESHVSIESYCAVCKEPFDLGSEAKEMPCQHLYHPDCILPWLALRNSCPVCRHELPSDGPDASSQNGGQNGVNNQSGHEEEAAGLTIWRLPGGGFAVGRFTGGRRGGERELPVVFTEMDGGFNHNGAPRRIQWASRGNVARERNGLSRVLRNLFSCFGGGFSRNRRRALASSSSSSSDDRASQRSRSMSSRRMAVAFDEHNEPRRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D0H7</accession>
    <name>A0AAD5D0H7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">KRR-R motif-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_012745</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the KRR1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005820">
        <property type="protein sequence ID" value="KAI7751778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D0H7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22393">
        <property type="entry name" value="KH-I_KRR1_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22394">
        <property type="entry name" value="KH-I_KRR1_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000014">
        <property type="entry name" value="KRR1 small subunit processome component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000137">
        <property type="entry name" value="KRR1 small subunit processome component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041174">
        <property type="entry name" value="KRR1-like_KH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048550">
        <property type="entry name" value="KRR1-like_KH1_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048548">
        <property type="entry name" value="KRR1-like_KH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048549">
        <property type="entry name" value="KRR1-like_KH2_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024166">
        <property type="entry name" value="rRNA_assembly_KRR1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12581">
        <property type="entry name" value="HIV-1 REV BINDING PROTEIN 2, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12581:SF0">
        <property type="entry name" value="KRR1 SMALL SUBUNIT PROCESSOME COMPONENT HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17903">
        <property type="entry name" value="KH_KRR1_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21800">
        <property type="entry name" value="KH_KRR1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006515">
        <property type="entry name" value="KRR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="KRR1 small subunit processome component first KH" evidence="10">
        <location>
            <begin position="83"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="KRR1 small subunit processome component second KH" evidence="11">
        <location>
            <begin position="165"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="35"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="276"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="317"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="40"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="276"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF17903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF21800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7751778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="410" mass="47043" checksum="A79A09288C130158" modified="2024-05-29" version="1" fragment="single">PPSSSPTQVPLFLLSNHQFQQSEFVAMDTIEVNDDNNIGKGKRHKGKHDKPKPWDDDEVLKKQNIDRWAVIAAPDEPCLLEVSSFSTLFPQYRETYLQSAWPEVKSKLKEHGIACELDLVKGSMTVSTTRKTKDPYIIVKARDLIKLLSRSVPVAQAVKILNDEMQCDIIKIGGLVRKKEKFVKRRQRLIGPDSQTLKALEILTRCYILVQGNSVSAMGSFKGLKQVRRVVEDCMLNKMHPVYNIKVLMMKRDLANNPELANENWDRFLPKFKKKNVKQKKVKTKEKKEYTPFPPPQPPSKIDMQLDSGEYFLSDKKKSAKKWQERQEKQAEKTAENKRKREEAFKAPEEARVEDDDKNHKDDIAAMTASLKNKAKEFGNRKSASDEIDAEMYIAASGQLSSKKKRKNKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D1K8</accession>
    <name>A0AAD5D1K8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_013199</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005988">
        <property type="protein sequence ID" value="KAI7751084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D1K8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33400:SF2">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33400">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="4">
        <location>
            <begin position="297"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="2">
        <location>
            <begin position="297"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="329"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="340"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7751084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="354" mass="39291" checksum="406EDE9DE977826A" modified="2024-05-29" version="1">MLGKRGSELRIGCSRTYIHNIRQIDWACPPRFLVDPDWKMEAGSESQETHIQLQRESRIAEVIYYHKSDIPPNPYVAPNAFDLNDDSRILVIPLEVIKDETSPNDPLIPPEPTVTSTRPVLQSDEVIGTAVALVALIKSKEPGSQIDLELLVQLLCNPGMLEKLTKEHGLSANQSSVITRAQPRECSSVPLPVPMPAVGGCSTWFRSTPFSRSLPQGVRNMIVQIHSNSESQNPSSNRKVDVSKLRKLVNEYGLGDDLVKRGPAVVKDADYYRRLISQHGMSQENDKPQESVKLTASQEQRLCVYFNSPKGCRRGSGCRFQHVKGGFGGGGQTVEEQHGAKRMKLSGRSFGRHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D379</accession>
    <name>A0AAD5D379_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_027811</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005716">
        <property type="protein sequence ID" value="KAI7752250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D379"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35699">
        <property type="entry name" value="F2J10.10 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35699:SF1">
        <property type="entry name" value="F2J10.10 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="153"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7752250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="214" mass="24156" checksum="FA9D83AECC36BF68" modified="2024-05-29" version="1">MASIQILKSGLYPFSALQPSSRRNERLICYDSRRVVKFSSQVSVNRRFVVSCKGQGTDDNDIKGEEPTESLFMKELKRRGMTPTSLLEESWSTLKDENITYTEEDGGFSNTNSVSTDFEKALSNQRERSMALNSEGLEGLIPRAKVLLTLGGTYFLAFWPLILVTVVSFSAVYLYFGPKFVHDATTRQVNLPQYVDPYALLEDQRISETAPQLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D534</accession>
    <name>A0AAD5D534_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_000459</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cyclic nucleotide-gated cation channel (TC 1.A.1.5) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010003750">
        <property type="protein sequence ID" value="KAI7754411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D534"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005216">
        <property type="term" value="F:monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.630:FF:000003">
        <property type="entry name" value="Cyclic nucleotide-gated ion channel 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000024">
        <property type="entry name" value="Cyclic nucleotide-gated ion channel 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.630">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45651">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-GATED ION CHANNEL 15-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45651:SF114">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-GATED ION CHANNEL 16-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0407">Ion channel</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="106"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="138"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="176"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="306"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="12">
        <location>
            <begin position="410"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="611"/>
            <end position="638"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7754411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="638" mass="73457" checksum="D2BFBF187764387C" modified="2024-05-29" version="1">MNYIRLPSTTFLSRPISSSFRNNPNNSWWTQIFDPGGQFITRWNYMFLIICLNALFVDPLYFLVPEIARGKYCMRTAYVAPNSRVFGRGDLVMDPRKIALRYLRRGFAIDLAAALPLPQLFIWFVIPAVSTAARANHSISLIIIIQYIPRLFVIFPLNQRINKTTGVIAKTAWVGAAYYVMLYMLASHVIGASWYLMAIERQYSCWKEECAKEENVTYPCRRVYFDCDHVNDPGRDKWLKQTQVLTNCSSENSPVNFTFGIYAPAFSEGVTSATFFEKYFFCFWFGLKSLSSYGQNLETSTSVGETIFGCTVCLGGLVIFCLLIGNMQTYLQSTTARLEEWRVKRRDTEEWMRHRQLPPDLQDKIRRFVQYKWLATRGVDEEDILHALPVDLRRQIQRHLCLALVRRVPFFSQMDDDQLLDVICEHLVQSLSTKDQHLVREGDPVNEMTFVIRGELESSTTNGGRSGFFNSITLKHGDFCGEELLTWALTPESNVNLPSSTRTVKALTEVEAFALRAEDLRFVAKQFKRLHSKKLQHAFRYYSHQWRTWGACLVQNAWRKYKRKKLAEELARQEGLYQNNNDDNDGGENDQNAQHLGATILASRFAKNTRKGISHKEGVGTSSPSMPKLFKPDEPDFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D5A4</accession>
    <name>A0AAD5D5A4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein SET DOMAIN GROUP 41</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_017963</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010003114">
        <property type="protein sequence ID" value="KAI7754501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D5A4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd20071">
        <property type="entry name" value="SET_SMYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47780">
        <property type="entry name" value="PROTEIN SET DOMAIN GROUP 41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47780:SF1">
        <property type="entry name" value="PROTEIN SET DOMAIN GROUP 41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7754501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="570" mass="62469" checksum="E8D28AD8E1DE4748" modified="2024-05-29" version="1">MEMEMVAAESIGIGEDLTPPLSPISYSLHDSFLHSHCASCFSPLPSSSSSSFPNHHHHLNVNVNVNYCSPSCESFHSPLHISSSAHLLSPHPSPSLQPSDLRVALLLLLQLKRPPHSSSSSYQYRICGLMTNRHNLLTNHHDHDLITNITNGAKAMAAATSTSTTADGNFVLEEAVLCAVLTNAVEVQDTTGRSLGIALYHSSFSWINHSCSPNASYRFLFLPHTHQHKQQQQRFLIAPPSSQPFEFYHSHLLTGLGLGLGLAAGPKIVVRSISMINKGDQVTVSYTDLLQPKELRQSELWSKYRFTCCCHRCVAHPLSYVDQRLQDICGTDDFVTECIDDAIDDYLCSNDHESCCTKLEDALSNHRTRLHPQNHLSLKAYTALASAYSSASAIAMDGEDGLKMKRFSAAYSLLLALQTHDLFLHESSLIASVSTFWIGAGESLINVAARLVSGRKWGGCGCGSIDIREVNLEVLKKQVLKCMSDIIGAGKVWKFLAVGNSYLEVVPMDLVLRWVESKTVGDDDDDECVEGQQVFGLGAHCLLYGGILANICGGNDSHLSCHARRLLCEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D5E9</accession>
    <name>A0AAD5D5E9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GDSL esterase/lipase At5g62930</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_021554</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005524">
        <property type="protein sequence ID" value="KAI7753169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D5E9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01838">
        <property type="entry name" value="Isoamyl_acetate_hydrolase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1110:FF:000002">
        <property type="entry name" value="isoamyl acetate-hydrolyzing esterase 1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045136">
        <property type="entry name" value="Iah1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14209:SF36">
        <property type="entry name" value="GDSL-LIKE LIPASE_ACYLHYDROLASE FAMILY PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14209">
        <property type="entry name" value="ISOAMYL ACETATE-HYDROLYZING ESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7753169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="248" mass="27745" checksum="8BE0BF7606D56C1A" modified="2024-05-29" version="1">MRPQIILFGDSITELSFKYGGWGASLTDTYSRKADILVRGYGGYTTRWALFLLHHIFPLSSTTPPVATTVFFGANDAALPGRTSERQHVPLEEYKENLRKIVRHLKECSPTMLIVLITPPPIDEEGQQQYAISVHGEKAPERTNEVTGNYANGCVEVAKELGVSSVNLWSKMQATQGWQKKYISDGLHLTPDGNNVVYEEVVKAFTGAWLSANEMPADFPHHMEIDPQNPAKAFQERCLISTFTKKDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D761</accession>
    <name>A0AAD5D761_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">SEC12-like protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_018011</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010003968">
        <property type="protein sequence ID" value="KAI7754252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D761"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003400">
        <property type="term" value="P:regulation of COPII vesicle coating"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000612">
        <property type="entry name" value="SEC12-like protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045260">
        <property type="entry name" value="Sec12-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23284">
        <property type="entry name" value="PROLACTIN REGULATORY ELEMENT BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23284:SF0">
        <property type="entry name" value="PROLACTIN REGULATORY ELEMENT-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="3 11" id="KW-0853">WD repeat</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="345"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="170"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7754252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="370" mass="40689" checksum="3CB9F42637784F3F" modified="2024-05-29" version="1">MSNYKKYGVPFYGAAFVPRNAFGSEHKHEEEEEDDYVLFCGGGGEGRSGIPNALVISSFDRASDTLDDQPVSQLGTDDNIPYRMAVHPGGEGVICSLPKSCRWFEWETTTNENVSTLSLKQSEKVLYQLEHIGQQLAVTFSHDGSLLALGGEDGKLRAFKWPSMESLVDVPDAHTSIKNLDFSPDGKFLVSVGSGGPGRVWDLSSSTFKASLPKEKDEVFGFCRFSQNHVNDQVLYVTAMRDRGGSIVKWNTTTWKRISSKHIVRDPISAFDVSDDGKFLAIGTAEGDVFILNAISMKVHTAVKKAHLGLVTALAFSHDSRALASASWDSSGRVTQIKETKKNRLNIWIILFIILLAAALYYAKTEGLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D7C4</accession>
    <name>A0AAD5D7C4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_000059</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005584">
        <property type="protein sequence ID" value="KAI7752900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D7C4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7752900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="80" mass="9404" checksum="F6EEF5E88B673711" modified="2024-05-29" version="1" fragment="single">FRRQPNGWECGYCVMMAMYDFVIYNGEHMLVDKTAMVRQGEINEFVERTLKVEVEFWLVSAPEILWLSWWINKAAPSQGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D7X0</accession>
    <name>A0AAD5D7X0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Gnk2-homologous domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">M8C21_019912</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">AA19_3_7</strain>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010001130">
        <property type="protein sequence ID" value="KAI7755433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D7X0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23509">
        <property type="entry name" value="Gnk2-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.430.20:FF:000003">
        <property type="entry name" value="Cysteine-rich RLK (RECEPTOR-like protein kinase) 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.430.20">
        <property type="entry name" value="Gnk2 domain, C-X8-C-X2-C motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002902">
        <property type="entry name" value="GNK2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038408">
        <property type="entry name" value="GNK2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002:SF1073">
        <property type="entry name" value="CYSTEINE-RICH RECEPTOR-LIKE PROTEIN KINASE 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002">
        <property type="entry name" value="RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE SD1-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01657">
        <property type="entry name" value="Stress-antifung"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51473">
        <property type="entry name" value="GNK2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5 15" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042085197" description="Gnk2-homologous domain-containing protein" evidence="15">
        <location>
            <begin position="25"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="264"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="Gnk2-homologous" evidence="16">
        <location>
            <begin position="24"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="Gnk2-homologous" evidence="16">
        <location>
            <begin position="131"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="356"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAI7755433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="544" mass="59394" checksum="809EEC04D4FC447D" modified="2024-05-29" version="1">MSILIGKLLLRLSFIFMLSTKTTSFTYYYCEKDGNFTTNSTYRTNLYTALSTLPTTNTGLGFFNQSIGQGSDRVYTSALCRGDINPDACDSCLNNSLVNLPEMCPNQKGAIGYHDACLLYYSNKSIFGNTSGGGVILYNAQNASDRDRFTVAPGPLLNKLIVEAAGGGLLRKFSSSNTTEPGSNITIYGLVQCIPYLTGPQCSDCLKNALDVFVSQYSWSIGGRLLQPKCNFRYEIYPFFNSSTPVTSPPPPPPPQGKTSNSTVIIVVVVVTICVGVVTASIYIFLRSRKKKKQMLHTNSVDNEMLDDSIVESLQYNFNLVKAATNDFSIENKLGQGGFGAVHKGKLGDDQEVAVKRLASDSGQGDIEFKNEVFGYMAPEYAMHGQFSVKSDVFSYGVLVLEMITGQKNQCFKNGESIEDLLSFAWESWQNGTVTKMIDPILSIGSGSLHEIIRSIHIGLLCVQENVAERPTMDSVLRMLNSFSVTLRVPSEPAFFVHGNTSSKMTHLTEYGSSTGSHGLEKSKIAKSSQISIVGVSISETIPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D848</accession>
    <name>A0AAD5D848_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_014094</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010001017">
        <property type="protein sequence ID" value="KAI7755553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D848"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000178">
        <property type="entry name" value="serine/threonine-protein kinase PBS1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47985">
        <property type="entry name" value="OS07G0668900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47985:SF1">
        <property type="entry name" value="PROTEIN KINASE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="37"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="130"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7755553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="325" mass="36493" checksum="578DD9A3DF863F9F" modified="2024-05-29" version="1">METNVTKPPTRTHIKSNQHHFLHHNHRDHHIQPRTTILMVLVPVVIVILLLAISLIVAYLRRAKSPKSNGTAYANKSCMFNAHSIITFNGTKDMKRGGTYKGSPRSLRSAASKGVQVFTYKELQSATDNFNQTNLVSKGGFGYVYRGMLRDGTLSIIKMLHREGKQGERAFRSEVDLLTRLHSPYLVDLLGYCADNQHKLLVFEYMPNDVYSYGVVLLELLTGRVPIDTNRPPGEHVLVSWALPKLTNRAAVLEMVDPNLHGQFSRKDLVQVAAIAAMCVQTEADYRPLMTDVVQSLIPIVHSISFGCSTSSFRFSKRISPRSYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DAE3</accession>
    <name>A0AAD5DAE3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">LOB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_017805</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LOB domain-containing protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000591">
        <property type="protein sequence ID" value="KAI7756092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DAE3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004883">
        <property type="entry name" value="LOB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31304">
        <property type="entry name" value="LOB DOMAIN-CONTAINING PROTEIN 38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31304:SF43">
        <property type="entry name" value="LOB DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03195">
        <property type="entry name" value="LOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50891">
        <property type="entry name" value="LOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LOB" evidence="2">
        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7756092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="178" mass="19151" checksum="5F64819C1BDE73CA" modified="2024-05-29" version="1">MSCHGCRVLRKRCGENCVLRPCLDWIDSPEAKARATLFVSKFFGRGDLFSFISSVPVHKRNALFQSLLFEAVGRTVNPVNGAIGLLSTGNWHLCQAGVQTVLAGGTPAILSPQIPVTNRSSSVSQATGALSAMMIGNPAGDDEQCYSMMPESDVSNVPMSFRCSDGEDQEPKLLNLLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DBQ9</accession>
    <name>A0AAD5DBQ9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_014468</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. KIN-7 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000259">
        <property type="protein sequence ID" value="KAI7756797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DBQ9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021881">
        <property type="entry name" value="NACK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968">
        <property type="entry name" value="CENTROMERE PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968:SF55">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIN-7H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11995">
        <property type="entry name" value="DUF3490"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 7" id="KW-0493">Microtubule</keyword>
    <keyword evidence="5 7" id="KW-0505">Motor protein</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="10">
        <location>
            <begin position="1"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="450"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="579"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="229"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="450"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="579"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="784"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7756797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="784" mass="87959" checksum="DF3D5A279EEBC1D5" modified="2024-05-29" version="1" fragment="single">EMGENGDAEKIFVSVRLRPLNGREIVTNDVSDWECFDNNTIVYNNGNVYQDGAKDVVLSVVGGINASIFAYGQTSSGKTFTMTGITEYTLADIYDYVQKTIESSPNNYLGRDSASTLVSTVNFVDLAGSERASQSLTAGTRLKEGCHINRSLLTLGTVIRKLSKGRSGHIPFRDSKLTRILQSSLGGNSRTAIICTMSPARSHVEQSRNTLLFASCAKEVSTNAQVNTVISDKALVKHLQRELDRLENELRTPSSNRLSPDTSTLLREKDNQIEKMLKQIEDLTAERDQARSQVQDLLRVVEDNEISLIRLGPDNYPQLRVQMISETENYMPETSIVVDPRQSFDGSVRTLGSSRYSPRRSESSFEENYIPVTEFEENSPTDSTPIRPMLRIAQSSETDSCHDWNDVEEKSNGSSHELCKVVRCIESEDATPKAYVQSYRSSPDTKIRISSETVSRDDHNGSPLEHMSPESCVDEELNEHNKVVPLPLKEAQEVETPLKEDESGNKPPLNEQLELNGSIEDDYVLVVPQSFKLPKSRSCNDGIHATLLNKLEHDSTPQNGGTPHSCTFNYGVELEKGTIDHNSEEPEKKTLIEEEPVEAQDDKQVDDNQITTTEETKTVKSIGLDPIVNSFRATSWRSEFKRLQKEIVQRLAFVKDMFSRVNAAVDDGQTITRSTTEKALRREREMLTKQMHKKLTEQEREGLFLRWEIGLNTKYRRIQLANKLWSQVDDIDHVADSAVVVAKLVGLISDPGHAPKEMFGLNLTPPNSKKSYSFKRSSACICEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DC27</accession>
    <name>A0AAD5DC27_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Queuine tRNA-ribosyltransferase catalytic subunit 1</fullName>
            <ecNumber evidence="6">2.4.2.64</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Guanine insertion enzyme</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">tRNA-guanine transglycosylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_023173</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming queuine, allowing a nucleophilic attack on the C1' of the ribose to form the product.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>guanosine(34) in tRNA + queuine = queuosine(34) in tRNA + guanine</text>
            <dbReference type="Rhea" id="RHEA:16633"/>
            <dbReference type="Rhea" id="RHEA-COMP:10341"/>
            <dbReference type="Rhea" id="RHEA-COMP:18571"/>
            <dbReference type="ChEBI" id="CHEBI:16235"/>
            <dbReference type="ChEBI" id="CHEBI:17433"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:194431"/>
            <dbReference type="EC" id="2.4.2.64"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="6">Heterodimer of a catalytic subunit and an accessory subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the queuine tRNA-ribosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.64" evidence="6"/>
    <dbReference type="EMBL" id="JAMZMK010000286">
        <property type="protein sequence ID" value="KAI7756709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DC27"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008479">
        <property type="term" value="F:tRNA-guanosine(34) queuine transglycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.105">
        <property type="entry name" value="Queuine tRNA-ribosyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00168">
        <property type="entry name" value="Q_tRNA_Tgt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004803">
        <property type="entry name" value="TGT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036511">
        <property type="entry name" value="TGT-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002616">
        <property type="entry name" value="tRNA_ribo_trans-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00430">
        <property type="entry name" value="Q_tRNA_tgt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00449">
        <property type="entry name" value="tgt_general"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43530">
        <property type="entry name" value="QUEUINE TRNA-RIBOSYLTRANSFERASE CATALYTIC SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43530:SF1">
        <property type="entry name" value="QUEUINE TRNA-RIBOSYLTRANSFERASE CATALYTIC SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01702">
        <property type="entry name" value="TGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51713">
        <property type="entry name" value="tRNA-guanine transglycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 6" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="5 6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="tRNA-guanine(15) transglycosylase-like" evidence="7">
        <location>
            <begin position="8"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="RNA binding" evidence="6">
        <location>
            <begin position="241"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="RNA binding; important for wobble base 34 recognition" evidence="6">
        <location>
            <begin position="265"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="86"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="260"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="86"/>
            <end position="90"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="298"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="300"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="303"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="328"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7756709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="399" mass="44184" checksum="55617BECBA12D1E2" modified="2024-05-29" version="1">MVLGRFNRARAARLILPHFVCETPLFMPVGTQGTIKGLTTNQLEDIGCQIILGNTYHLALRPTSELIDELGGLHKFMNWPRALLTDSGGFQMVSLLHLADITEEGVTFQSPVDGKPMLLTPEESIQIQNRIGADIIMALDDVVKTTITGPRIEEAMYRTLRWIDRCIAAHKRPKEQNLFGIVQGGLDPVLRDICVQGLVERNLPGYAIGGLAGGEDKDSFWRVVAQCTASLPESKPRYVMGVGYPLDIVVCSALGADMYDCVYPTRTARFGTALVPEGTLRLKHQSMADDTRPIDSTCECMVCKNYTRAYIHCLVTKDAMGSQLLSYHNLYYMMQLSRNLHSSIIEGTFPKFVCDFLQKMFPEGDVPEWVCNAMEVAGIDISSCCTPSNTKLKIYASSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DFV5</accession>
    <name>A0AAD5DFV5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_014594</name>
        <name evidence="4" type="ORF">M8C21_014597</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LEA type 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000209">
        <property type="protein sequence ID" value="KAI7756977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAMZMK010000209">
        <property type="protein sequence ID" value="KAI7756980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DFV5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009793">
        <property type="term" value="P:embryo development ending in seed dormancy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005513">
        <property type="entry name" value="LEA_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33493:SF2">
        <property type="entry name" value="LATE EMBRYOGENESIS ABUNDANT PROTEIN 46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33493">
        <property type="entry name" value="LATE EMBRYOGENESIS ABUNDANT PROTEIN 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03760">
        <property type="entry name" value="LEA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="36"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7756977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7756980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="67" mass="7319" checksum="6D875778B9079E68" modified="2024-05-29" version="1">MTAHDPMQKEMAREKKEERKHEAEFEKQAAKEENAAQKQAGTMGTGTGTRTYTSTNVTGHHTGTGGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DGQ3</accession>
    <name>A0AAD5DGQ3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Two-component response regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">M8C21_007152</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">AA19_3_7</strain>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Transcriptional activator that binds specific DNA sequence.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ARR family. Type-B subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000128">
        <property type="protein sequence ID" value="KAI7757400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DGQ3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009736">
        <property type="term" value="P:cytokinin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17584">
        <property type="entry name" value="REC_typeB_ARR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000007">
        <property type="entry name" value="Two-component response regulator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045279">
        <property type="entry name" value="ARR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006447">
        <property type="entry name" value="Myb_dom_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017053">
        <property type="entry name" value="Response_reg_B-typ_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01557">
        <property type="entry name" value="myb_SHAQKYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43874:SF136">
        <property type="entry name" value="RESPONSE REGULATOR AND TRANSCRIPTION FACTOR RR-A-TYPE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43874">
        <property type="entry name" value="TWO-COMPONENT RESPONSE REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036392">
        <property type="entry name" value="RR_ARR_type-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0010">Activator</keyword>
    <keyword evidence="4" id="KW-0932">Cytokinin signaling pathway</keyword>
    <keyword evidence="7 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3 12" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 11" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 11" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="Response regulatory" evidence="14">
        <location>
            <begin position="21"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="15">
        <location>
            <begin position="186"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="140"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="515"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="579"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="140"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="172"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="595"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="12">
        <location>
            <position position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR036392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAI7757400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="605" mass="66965" checksum="9A496F62E4B5DB71" modified="2024-05-29" version="1">MTVEEIRGSDHEIDRFPIGMRVLAVDDNPTCLKLLEGLLRKCQYHVTTTNQAITALNMLRENRNRFDLVISDVYMPDMDGFKLLELVGLEMDLPVIMLSGNGDRNLVMKGVTHGACDYLVKPVRLEELRNIWQHVIRRKVESKSKNNHEKSDQGKEGGDQNEKLNRKRKDEDTDEENGNESDDPTTKKKARVVWSIDLHRKFVAAINQLGIEKAVPKRILDLMNVDGLTRENVASHLQKYRLYLKRISQQANMVVTFGGNNDASSYMRMSPLDGLGDFRTLSCSGRLPNASYAPPGMLGRLNSATGVALHSLTAPPLVQPNHTQTKLQPVVSSNHQNVNLFQGIPPSFDHDQRQYCNKPNTQLTDVGSFDESRIFTGTNLAGFSSIDEPRMFRGSSTFTDPISVLANQAGVKLTSTGTESFNHLSDSSGFLDQNGNQPPNFSSNLNLQPLAESYSYPAVINGGFSSNITTAAAPIEPHGGGLVGDIVQNVNRSLPNQKGGSRSKQDYSQYSQNALPTQATSALASYDSLSGPVSHSRPNGSRSIPFQTEQIKLQSGFVSNKSYGSLDDIIPGMMKQEQDTTMPMNGEFGYDPYIDPDREYNDESR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DHD9</accession>
    <name>A0AAD5DHD9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein LURP-one-related 8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_001633</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LOR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000071">
        <property type="protein sequence ID" value="KAI7757730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.200">
        <property type="entry name" value="LURP1-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007612">
        <property type="entry name" value="LOR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038595">
        <property type="entry name" value="LOR_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025659">
        <property type="entry name" value="Tubby-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31087">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31087:SF138">
        <property type="entry name" value="TUBBY-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04525">
        <property type="entry name" value="LOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54518">
        <property type="entry name" value="Tubby C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7757730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="197" mass="22057" checksum="9758E881805A2143" modified="2024-05-29" version="1">MHFTAIIVAAVMRQRCRGNASEGPLVLTVWKKSLLFNCYGFTVFDSNGDLVFRVDNYTAGSKAEIVLMDASGRSLLTIRRKVKFIGQLARIRRGYNRESXXXXTVTKNVNLLNTKSLAHVSSTGSKTKKVIYEIEGSYTQRCCSVYDDKRRRVAEIKRKEAVGGVTFGGDVFRLFVQPDIDPSVAMALVVVLDQMFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G185</accession>
    <name>A0AAD5G185_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ENTH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_011012</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Clathrin-coated vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <location evidence="3">Clathrin-coated pit</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012147">
        <property type="protein sequence ID" value="KAI7724869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G185"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030136">
        <property type="term" value="C:clathrin-coated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005545">
        <property type="term" value="F:1-phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032050">
        <property type="term" value="F:clathrin heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048268">
        <property type="term" value="P:clathrin coat assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072583">
        <property type="term" value="P:clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006900">
        <property type="term" value="P:vesicle budding from membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16987">
        <property type="entry name" value="ANTH_N_AP180_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000019">
        <property type="entry name" value="Clathrin coat assembly protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.150:FF:000005">
        <property type="entry name" value="putative clathrin assembly protein At2g25430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.150">
        <property type="entry name" value="ANTH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011417">
        <property type="entry name" value="ANTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014712">
        <property type="entry name" value="ANTH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048050">
        <property type="entry name" value="ANTH_N_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045192">
        <property type="entry name" value="AP180-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22951:SF62">
        <property type="entry name" value="ASSEMBLY PLANT-LIKE PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22951">
        <property type="entry name" value="CLATHRIN ASSEMBLY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07651">
        <property type="entry name" value="ANTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00273">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89009">
        <property type="entry name" value="GAT-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50942">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0168">Coated pit</keyword>
    <keyword evidence="8" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="4" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENTH" evidence="9">
        <location>
            <begin position="24"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7724869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="515" mass="57741" checksum="099CB245EF00D429" modified="2024-05-29" version="1">MSSSTIRKAIGVVKDQTSISIAKVAGNVAPDLEVLIVKATGHDKEPAEEKYIREILHLISQNRGYVSACVYNISKRLGKTHDWVVALKALMLVHRLLVDGNPVFGQEMMFASRKGARVLNMSDFRVEALSSYWDHSTFVKSYALYLDQKLEFIAFERKLRPTGFRDDHGYGIRQKSKSYGDLYESARGEKRETKVAITPVRDMKPDRVLERLNKLLRLIDRVLSCRPVGNAKTSKMVLAALYLVLNESFWIYADICEALGVLLDRFPEMEFGNCVRTFDQYVNAAKTIDELVDFYSWSKELGIARASEFPQVQKVTDKILGTLERFLKDKKKKLKKNMEDCCKENGPSPPALVAATENLLDLEDDIVPEAANDGKTLAISLFSGPKTIEADWELALVESTSNPSKLKTCGGIDPVMLNEMYDQGAVTQRISYSSAPAVLALPAPSETKVQTVVDPQDPFAASLMVPPPPYVQLADMEKRTRFLVQEQQQVWQPYGSNVGMMNGIVKQAGYNYAHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G198</accession>
    <name>A0AAD5G198_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="2">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_015461</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010012184">
        <property type="protein sequence ID" value="KAI7724600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G198"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23116">
        <property type="entry name" value="RING-H2_AIRP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46463:SF10">
        <property type="entry name" value="OS01G0926200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46463">
        <property type="entry name" value="ZINC FINGER, RING/FYVE/PHD-TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="10">
        <location>
            <begin position="165"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="102"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="102"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7724600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="244" mass="27401" checksum="D89EDDD160574A6A" modified="2024-05-29" version="1">MDTICCCQCGDDFEEFPTNAIYRHCICLRFFFHQLFAGYGALFQRIEGLPVSSPIQGTASSSAVALSLPTSLNVENHSATRPMPYDSDHRFTHLPRGLVSRREKSMTHFQEDSQSGMEFAKRKPGLDSADEDSKSGNLESLDKSLKVKASEGLLYEQSSDNEDVCPTCLDEYTLENPKIVTQCSHHFHLGCIYEWMERSDTCPMCGKVMEFCESPVPRMSNAVHEPMRDCELGLDFELSQLGSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G1C3</accession>
    <name>A0AAD5G1C3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Amino acid transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_004627</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the amino acid/polyamine transporter 2 family. Amino acid/auxin permease (AAAP) (TC 2.A.18.2) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012171">
        <property type="protein sequence ID" value="KAI7724750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G1C3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000033">
        <property type="entry name" value="Lysine histidine transporter 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48017">
        <property type="entry name" value="OS05G0424000 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="59"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="109"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="153"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="176"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="259"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="305"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="348"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="374"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="407"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="10">
        <location>
            <begin position="30"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00061463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7724750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="441" mass="49451" checksum="92D6708444221754" modified="2024-05-29" version="1">MESGSDSKFDTRTAEEKALDEWLPVTSSRNAKWWYSAFHNVTAMVGAGVLTLPFAMSELGWTFGITVLILSWVITLYTLWQMVEMHEMVPGKRFDRYHELGQHAFGEKLGLYIVVPQQLVVEVGTCIVYLVTGGKSLQKFEQLLCPDCKPMRTTYFILIFSSVHFVLAHLPNFNSISVVSLAAAVMSLSYSVIAWVAALDRGRNENVDYSLRSKTPVGQMFDMFTALGDVAFAYAGHNVVLEIQATIPSTPEKPSKGPMWLGCVVAYIIVAICYFPVAIIGYYVFGNKVDDNILITLDHPRWLIAMANMFVIIHVIGGYQIFSMPVYDMIETYLVKSKNFQPSTPLRFTVRTSFVAITTFIAITFPFFGGLLGFLGGFALAPTSYYLPCIIWLIVKKPRKWSLSWWINWICIVIGVLLTILAPIGGLRSIIKSAGTYKFYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G3A2</accession>
    <name>A0AAD5G3A2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_033382</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011407">
        <property type="protein sequence ID" value="KAI7727254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G3A2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22157">
        <property type="entry name" value="F-box_AtFBW1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013187">
        <property type="entry name" value="F-box-assoc_dom_typ3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017451">
        <property type="entry name" value="F-box-assoc_interact_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050796">
        <property type="entry name" value="SCF_F-box_component"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01640">
        <property type="entry name" value="F_box_assoc_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31672">
        <property type="entry name" value="BNACNNG10540D PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31672:SF13">
        <property type="entry name" value="F-BOX PROTEIN CPR30-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08268">
        <property type="entry name" value="FBA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="3"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7727254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="379" mass="43152" checksum="52A1CEC69169F86B" modified="2024-05-29" version="1">MSPSGSINIPLEITEAILRLLPAKSLGRFKSVSKTWNSLISDPNFIKTHLLHTHQTSKLIGLSTTGSLYSIDISETLDSDDDDDDDDDILATAKELSIRSPPIRWKEIFGSCNGLLLAKDENDTIFMINPTTQDLWKVPLCPFAPPPDEEYEEEVYVTYGFGYDSSTDDYKIVSISFWHHHSMQHDSSETFASVYSLRNNSWRKLRDYDYHEHDHTVLDVINGAVINQTLHWLARHSEIVGFNLANEEFNEMKFPDTIGMYTSTFSSMVDVGGKLGVFIWSDAGFECCVMEEYGVWESWTRVRIHGVERDRVPWPECLLDGRSREIVFQEKEGKVLVYNIDERRFRDVRIVGGPGGTVNVYGGTFVESLESPKRIMIAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G3B0</accession>
    <name>A0AAD5G3B0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_004727</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011765">
        <property type="protein sequence ID" value="KAI7726056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36807">
        <property type="entry name" value="PHOSPHOGLYCOLATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36807:SF2">
        <property type="entry name" value="PHOSPHOGLYCOLATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7726056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="529" mass="59658" checksum="DE3F6AB49A8FBA05" modified="2024-05-29" version="1">MTEYVAVRPFIKLPVLGTELNFMPNGLYAQNRSRKCYAGDGRGMQAVHKSGGFCFTNLKTDRKLKANHMKCYCLGTLMSTAGDSFGWIHITDRVLLMTSIFLAHMAVAVPADGPFLGSGRKKLSDDVAPDGTPYSGSAMKNDKDGEVALEFSWDIVKRKLTDSITAIENGTKLEKEVMESGQTANQPSSLSAIAVGPRFRLMWASFNWLRNEVENISRGASTTDLKNLQMVFSEILQRSSLSICTDWLKDELLLRNKKSIKEFTTPLVEKLKGDNSVLQNIRKSGKEDLYSELVYVLRFGSISFDSQECCYDYDFFASWGVYVLEDLVITLADGIASIYLELISVDSDMSNEVNNLGLNLCDLSTRALQRLRNEVSLNQWFVQNMETVASMYEDRFDLRILKSQPLTESTKGKTEKFGWLKNFSLGKSRPLGSPLCAAVISSVCVPVKRTKELRALVGWYALFLLYYVIGQVPSNGIXXXXCYQNQRCRLILFGLSDWAILRSHLHRYKAVIEVEVRSHLWFFLTIVMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G6P5</accession>
    <name>A0AAD5G6P5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_029264</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010982">
        <property type="protein sequence ID" value="KAI7729536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G6P5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="41"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="70"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7729536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="152" mass="16445" checksum="B43423DBE448206D" modified="2024-05-29" version="1" fragment="single">EQDRRDAKDEAERRKQQEKARAKGPGGGRNARKPVRKAAPKKPSAASAVVIEPMETSANSSMETGTVNNAPAAKPRGKAAGQKKAPAKSKGKATLVDEDDDEIPSLAERMAKQNLHSPQVDDEIVELEDRLAKHNIESSPDQEGKPCLYLIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G7S3</accession>
    <name>A0AAD5G7S3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Dicer-like protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_020331</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="6 7">Belongs to the helicase family. Dicer subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010412">
        <property type="protein sequence ID" value="KAI7731652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G7S3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004525">
        <property type="term" value="F:ribonuclease III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030422">
        <property type="term" value="P:siRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18034">
        <property type="entry name" value="DEXHc_dicer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000628">
        <property type="entry name" value="Endoribonuclease Dicer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.380:FF:000001">
        <property type="entry name" value="Endoribonuclease dicer-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000420">
        <property type="entry name" value="Endoribonuclease dicer-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.380">
        <property type="entry name" value="Dicer dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1520.10">
        <property type="entry name" value="Ribonuclease III domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038248">
        <property type="entry name" value="Dicer_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005034">
        <property type="entry name" value="Dicer_dimerisation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003100">
        <property type="entry name" value="PAZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036389">
        <property type="entry name" value="RNase_III_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14950:SF15">
        <property type="entry name" value="DICER-LIKE PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14950">
        <property type="entry name" value="DICER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03368">
        <property type="entry name" value="Dicer_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69065">
        <property type="entry name" value="RNase III domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51327">
        <property type="entry name" value="DICER_DSRBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50821">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="37"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="10">
        <location>
            <begin position="377"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="domain" description="Dicer dsRNA-binding fold" evidence="11">
        <location>
            <begin position="561"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="domain" description="PAZ" evidence="8">
        <location>
            <begin position="593"/>
            <end position="725"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7731652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="764" mass="86158" checksum="FB36BDF2253E4C30" modified="2024-05-29" version="1">MGSISHEISVLTAPFEALSITPDKDPRTIARGYQIELCQKALLENIIVYLETGCGKTHIAVLLIYEMICRTKKSKKDICVFLAPTVALVEQQAKVIQASIDVKVGIYCGSSNNLKGHRDWEKELEQFEVLVMTPQILLHNLSHCFIRIQHIALLIFDECHYAQFESNHPYAEIMKIFYKPDAPKLPRIFGMTASPIFGKGGSISSLETLLNAKVYTVDTREELDQFVSSPKVNCYYYGTDTHDSSDSHLTYVGKLETIKADCMLSLIKNIKDSSSLKNTKKALVRLHCNLEFCLQNLGLWGALQACRILLKGDLVERNAVIEANEQCGDDMLCGSYLSQVFAYFAAECKKDYSIEASDTEAILSSLDNLKFPFFSLKLLQLVNILSNFRAQSDMKCIIFVNRIITARSLAYILQQVTFLSAWRCSYLVGVHSGLKNMSRKTTNAILEKFRSGELNLLVATKVGEEGLDIQTCCLVIRFDLPETVASFIQSRGRARMPQSEYAFLVDRGNPKEMELIKNFHNDEAQMNNEISSRKSTSVYVDFEEKTYKVDSTGAIISSGSSVSLLHRYCSKLPHDEFFNPKPELSYSTHEADAVICYIKLPANAPIHQIRSSPQPSREAAKKDACLKACQRLHELGALTHYLLPDDEVDDENQDSSDSDCDDGTSYVNHYADKFGREFVHPKQPLLKAKQVFRLDNLLRKRGSGEAREKEEHFVELPPEICVLKILEALTTERCNEWFSLERLEVLGDAFLKFAVGRHVRSIGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G960</accession>
    <name>A0AAD5G960_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_028640</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009897">
        <property type="protein sequence ID" value="KAI7733855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G960"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7733855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="64" mass="7159" checksum="8BF91270333D4438" modified="2024-05-29" version="1" fragment="single">ETFHFEAGGGYILTCSCLIELLTLAKLASEPKLRGIRAGQFKRFFRESNKDPPSGVFMHTSLIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GAH6</accession>
    <name>A0AAD5GAH6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Germin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">M8C21_009100</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">AA19_3_7</strain>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May play a role in plant defense. Probably has no oxalate oxidase activity even if the active site is conserved.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Oligomer (believed to be a pentamer but probably hexamer).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Secreted</location>
            <location evidence="2 14">Extracellular space</location>
            <location evidence="2 14">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the germin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009671">
        <property type="protein sequence ID" value="KAI7734682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GAH6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02241">
        <property type="entry name" value="cupin_OxOx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000098">
        <property type="entry name" value="Germin-like protein 9-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006045">
        <property type="entry name" value="Cupin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001929">
        <property type="entry name" value="Germin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019780">
        <property type="entry name" value="Germin_Mn-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31238">
        <property type="entry name" value="GERMIN-LIKE PROTEIN SUBFAMILY 3 MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00190">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00325">
        <property type="entry name" value="GERMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00835">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00725">
        <property type="entry name" value="GERMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 14" id="KW-0052">Apoplast</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 12 14" id="KW-0464">Manganese</keyword>
    <keyword evidence="7 12 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Cupin type-1" evidence="15">
        <location>
            <begin position="35"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU366015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAI7734682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="186" mass="20047" checksum="D704F740E688CF34" modified="2024-05-29" version="1">MSITTQANDPDILTDFITPNSSAVDASFFTFSGLSGFYNPDPKNFTVIKATMNEFPALDGQSVSYAVLQFPPAGVNPPHTHPRAAELLVVLLGTLEVGFVDTTNVLYNQTLQEGDMFVFPKGLVHFQYNRNHNQSAIAISSFGSANAGLLSVPKSVFTTGIDENVLAMSFKTDVPTIQKIELGLKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GBL2</accession>
    <name>A0AAD5GBL2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">THH1/TOM1/TOM3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_017847</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the plant tobamovirus multiplication TOM1 protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009476">
        <property type="protein sequence ID" value="KAI7735489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GBL2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040226">
        <property type="entry name" value="THH1/TOM1/TOM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009457">
        <property type="entry name" value="THH1/TOM1/TOM3_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31142">
        <property type="entry name" value="TOBAMOVIRUS MULTIPLICATION PROTEIN 1-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31142:SF46">
        <property type="entry name" value="TOBAMOVIRUS MULTIPLICATION PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06454">
        <property type="entry name" value="THH1_TOM1-3_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="15"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="89"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="128"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="159"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="193"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="236"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="THH1/TOM1/TOM3" evidence="8">
        <location>
            <begin position="36"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF06454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7735489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="257" mass="30054" checksum="09742768B144ECA5" modified="2024-05-29" version="1" fragment="single">ITHSTVTDNRTTSNMYRAFLGPFTPTVIMMFTLKDASDWWNLLNDTPIWQDRIFRGIALLYGLVAIVAMMQLFRIQQRVPEYGWTTQKVFHFLNFVVNAVRCLIFVFRRDITHLKQEILQHILLDVPSLAFFTTYALLVLFWAEIYYQARAVSTDCLRPTFYTVNGIIYAIQIALWLVIWWKPVRVMVILSKMFFAGVCLFAALGFLLYGGRLFLMLQRFPVESKGRRKKLQEANICQTVGYVTSICFTCFLIRSFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GDU1</accession>
    <name>A0AAD5GDU1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Exostosin GT47 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_012088</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 47 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009258">
        <property type="protein sequence ID" value="KAI7736483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GDU1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047517">
        <property type="term" value="F:1,4-beta-D-xylan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080116">
        <property type="term" value="F:glucuronoxylan glucuronosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010417">
        <property type="term" value="P:glucuronoxylan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009834">
        <property type="term" value="P:plant-type secondary cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004263">
        <property type="entry name" value="Exostosin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040911">
        <property type="entry name" value="Exostosin_GT47"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11062:SF200">
        <property type="entry name" value="BETA-1,4-XYLOSYLTRANSFERASE IRX10L-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11062">
        <property type="entry name" value="EXOSTOSIN HEPARAN SULFATE GLYCOSYLTRANSFERASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03016">
        <property type="entry name" value="Exostosin_GT47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042202966" description="Exostosin GT47 domain-containing protein" evidence="6">
        <location>
            <begin position="23"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="domain" description="Exostosin GT47" evidence="7">
        <location>
            <begin position="49"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7736483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="396" mass="45233" checksum="BEF43E216347C55B" modified="2024-05-29" version="1" fragment="single">MSNSSWVFVLYVFFALFLNLDAFELRKGQKTERISGSAGDVLEDDPVGRLKVFVYELPSKYNKKILQKDPRCLNHMFAAEIYMHRFLLSSPVRTLNPEEADWFYTPVYTTCDLTPNGLPLPFKSPRMMRSAIQLISSNWPYWNRTEGADHFFIVPHDFGACFHYQEEKAIERGILPLLQRATLVQTFGQKNHVCLKDGSITVPPYAPPQKMQSHLIPPSIPRSIFVYFRGLFYDVGNDPEGGYYARGARAAVWENFKDNPLFDISTEHPTTYYEDMQRAVFCLCPLGWAPWSPRLVEAVIFGCIPVIIADDIVLPFADAIPWEDIGVYVDEKDVPNLDTILTSIPTEVILRKQRLLANPSMKQAMLFPHPAQPGDAFHQILNGLARKLPHDKSVYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GFL1</accession>
    <name>A0AAD5GFL1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase C1A papain C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_016146</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008604">
        <property type="protein sequence ID" value="KAI7739569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GFL1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02248">
        <property type="entry name" value="Peptidase_C1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039417">
        <property type="entry name" value="Peptidase_C1A_papain-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="3">
        <location>
            <begin position="21"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7739569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="229" mass="24834" checksum="71D264EB22B00DD6" modified="2024-05-29" version="1" fragment="single">VPSTLKSGQTTPFRYENVTAVPSSIDWRKKGAVTPIKNQGQCGSCWAFSTIAATEGITGITTGKLISLSEQELMDCDRSGEDQGCEGGYMDDGFDFIVKNKGINTEAAYPYKAVDATCNTKKEGVAAAKITGHEDVPANNEAALLKAVAMQPLYLAIWAGAGICFGVEMTWTMTQAKAGDFDGESRNMRLRVCFNFTKAPQFAIACVRRNSKLQALYFELVSMVLHCKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GHE0</accession>
    <name>A0AAD5GHE0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 13">Pectate lyase</fullName>
            <ecNumber evidence="6 13">4.2.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">M8C21_018026</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">AA19_3_7</strain>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Has pectate lyase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>Eliminative cleavage of (1-&gt;4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.</text>
            <dbReference type="EC" id="4.2.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="13">Binds 1 Ca(2+) ion. Required for its activity.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 13">Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 2/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the polysaccharide lyase 1 family. Amb a subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.2.2" evidence="6 13"/>
    <dbReference type="EMBL" id="JAMZMK010007923">
        <property type="protein sequence ID" value="KAI7742720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GHE0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030570">
        <property type="term" value="F:pectate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018082">
        <property type="entry name" value="AmbAllergen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002022">
        <property type="entry name" value="Pec_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045032">
        <property type="entry name" value="PEL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31683:SF159">
        <property type="entry name" value="PECTATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31683">
        <property type="entry name" value="PECTATE LYASE 18-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00544">
        <property type="entry name" value="Pectate_lyase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00807">
        <property type="entry name" value="AMBALLERGEN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00656">
        <property type="entry name" value="Amb_all"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13" id="KW-0106">Calcium</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 13" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Pectate lyase" evidence="15">
        <location>
            <begin position="1"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAI7742720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="229" mass="24255" checksum="88C43BE64F66F00F" modified="2024-05-29" version="1">MHDVKVNPGGLIKSNDGPAAPRAGSDGDAISISGSSQIWIDHCSLSKSVDGLVDAKLGTTRLTVSNSLFTQHQFVLLFGAGDENIEDRGMLATVAFNTFTDNVDQRMPRCRHGFFQVVNNNYDKWGSYAIGGSASPTILSQGNRFCAPDERSKKNVLGRHGEAAAESMKWNWRTNKDVLENGAIFVASGVDPVLTPEQSAGMIPAEPGESALSLTSSAGVLSCQPGAPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GJX6</accession>
    <name>A0AAD5GJX6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">EGF-like calcium-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">M8C21_033476</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">AA19_3_7</strain>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Cytoplasmic vesicle</location>
            <location evidence="16">Clathrin-coated vesicle membrane</location>
            <topology evidence="16">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="17">Prevacuolar compartment membrane</location>
            <topology evidence="17">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VSR (BP-80) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007186">
        <property type="protein sequence ID" value="KAI7745630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GJX6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030665">
        <property type="term" value="C:clathrin-coated vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000178">
        <property type="entry name" value="vacuolar-sorting receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026823">
        <property type="entry name" value="cEGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056858">
        <property type="entry name" value="VSR_TRX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22702">
        <property type="entry name" value="PROTEASE-ASSOCIATED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22702:SF1">
        <property type="entry name" value="PROTEASE-ASSOCIATED DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12662">
        <property type="entry name" value="cEGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25011">
        <property type="entry name" value="VSR_TRX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="15" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="462"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like calcium-binding" evidence="19">
        <location>
            <begin position="409"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="20">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00029430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00043947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAI7745630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="519" mass="58127" checksum="5C3B4556786A9A24" modified="2024-05-29" version="1" fragment="single">FVVENINLKVTSPDDSLKGSYKCALANFGIPRYGGTLRGLVKYPKENQDACNSFNDVSFKPDNPGGLPVFVLADRGGCYFSNKSWNAQNAGAAAILVTHETDDLLTMDLPEHDPTATYLENLTISSVFITNSLGISIKKALEDINPVLVTLDWTEALPHPDERVEYEFWTNSNDECGLKCDNQLKFVKTFKKVAQALEQNGHTQFTPHYITWYCPEEYTSSEKCKSQCINHGRYCAPDPEEDFSKGYTGKEVVVQNLIQACFYKAANESGKPWLWWDYVTDFSTHCQMKEQQYNKDCAHKVIASFEIETNECLVDNGGCWIDKVNNITACKDTFRGKVCECPIVNGVKYVGDGYTHCEPSAALRCQVNNGGCWEGSKEGRNYTACIGDHTKSCECPPGFKGDGVTSCEDINECKEKKACQCSKCKCKNTWGSFECSCAANLLYLKEHDICISTKDVSTRDGWGFGWVFFGLIAIGVVGYVFYKYRVRRYMDSEIRAIMAQYMPLDGDVDVPVTRSHGEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GJY1</accession>
    <name>A0AAD5GJY1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_001845</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007197">
        <property type="protein sequence ID" value="KAI7745572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GJY1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009742">
        <property type="term" value="P:brassinosteroid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000016">
        <property type="entry name" value="probable serine/threonine-protein kinase At4g35230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000069">
        <property type="entry name" value="probable serine/threonine-protein kinase At5g41260"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045845">
        <property type="entry name" value="BSK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058209">
        <property type="entry name" value="TPR_BSK1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45863">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE BSK5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45863:SF7">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE BSK5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25575">
        <property type="entry name" value="TPR_BSK1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0519">Myristate</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="1"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7745572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="355" mass="39756" checksum="156D8C1DB3B26ECE" modified="2024-05-29" version="1">MSNETLSKHLFHWESQPLKWAMRLRVALYLAQALEYCSSKGRSLYHDLNAYRILFDQDCNPRLSCFGLMKNSRDGKSYSTNLAFTPPEYMKTGRVIAESVIYSFGTILLDLLSGKHIPPSHALDLIREKNFQMLTDSCLEGDFSNDDGAELVKIASRCLQYEPRERPNAKSVVTSLTALQKQAEVSSLVLMEITQITPAESTKQTDLSPFGEACSRMDLTAIHEMLEKVGYKDDEGTATELSFQMWTNQLQDALNGRKRGDTAFKAKDYTAAIESYTSFIDNGTMISPTVYARRCLCYLMNNKAQEALGDAMQAQVASPNWSTALYLQAAALFSLGMENDAQEMLKDGSLLEVKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GL19</accession>
    <name>A0AAD5GL19_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Pre-mRNA-splicing factor SPF27 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_011251</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SPF27 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007732">
        <property type="protein sequence ID" value="KAI7743488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GL19"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008409">
        <property type="entry name" value="SPF27"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13296">
        <property type="entry name" value="BCAS2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13296:SF0">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR SPF27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05700">
        <property type="entry name" value="BCAS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="157"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="17"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7743488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="298" mass="34489" checksum="87BD6B9D5EB69506" modified="2024-05-29" version="1">MATNTANNGDTVMLLEAPPSSRPSWSSSAAQIDALPYIDDEYGDPKVKAEVDRLIEEEMRRSSKRPSDYLKDLPPVAKFNFENYPMLAREYERVRTGKPMVPLDMSRYGLDVPTRNDETAWKQALQKSQRLVQHQVIRLENLDLMSKYGAEVWKQHNQRLEAFLSRMQAQATELNEKIEIVNRERKYHQQNTAYELNALSAQWKELCEKNISIEAACVQIENHLEQLKSEAAERGILVFMPHGENMLQFIANTSQEWDDEMETLLHQAYISGKDFESVPKYTNRSLNGYDMFYMAPSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GLG1</accession>
    <name>A0AAD5GLG1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_019370</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006776">
        <property type="protein sequence ID" value="KAI7747342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GLG1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.34.10:FF:000001">
        <property type="entry name" value="leishmanolysin-like isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.132.10:FF:000001">
        <property type="entry name" value="leishmanolysin-like peptidase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.170.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.132.10">
        <property type="entry name" value="Leishmanolysin , domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.34.10">
        <property type="entry name" value="Leishmanolysin domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036790">
        <property type="entry name" value="Frizzled_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001577">
        <property type="entry name" value="Peptidase_M8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10942">
        <property type="entry name" value="LEISHMANOLYSIN-LIKE PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10942:SF0">
        <property type="entry name" value="LEISHMANOLYSIN-LIKE PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23106">
        <property type="entry name" value="EGF_Teneurin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01457">
        <property type="entry name" value="Peptidase_M8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00782">
        <property type="entry name" value="LSHMANOLYSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63501">
        <property type="entry name" value="Frizzled cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 8" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="EGF-like" evidence="10">
        <location>
            <begin position="580"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="284"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="283"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="287"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="363"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="584"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="602"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR601577-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR601577-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7747342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="767" mass="84637" checksum="806A3879CD0DA0CF" modified="2024-05-29" version="1" fragment="single">MLNGAPFLDITISGFELSGIRIMAKYDQDRLQRKGPETGSVNIASHSCIHDEIIEQRRKKSGHKVYSVTPQVYQAKGTEPLHRNERTLLEIPKVSKFPKNAKQPIRIYLNYDAVGHSPTRDCRSVGDVVKLGEPPEASNLETSSCNPHNDPPMFSDCWYNCTSDDIVEEDKRRRLHRALGRTTDWFRRALAVERVRGNLRLSGYSACGQDGGVQLPRAYVEEGVADADLVLLVTTRPTTGNTLAWAVACERDQWGRAIAGHVNVAPRYLTAEAETLLSVTLIHEVMHVLGFDPHAFTHFRDERKRRRSKVTEQTMYEKLGRMVTRVVLPHVIMHSRHHYGTYSESFTGLELEDGGGIGTSGSHWEKRLLMNEIMTGSVDTRSVVSKMTLALLEDSGWYEANYSMADRLDWGSNQGTEFVTGPCNLWKGAYHCGHSSLADFCTYFVAYSDGSCIDANSAREPDKMLGEVRGSDSRCMASSLVRNGFVRGSMTQGNGCYQRRCVNNTLEVAVDGIWKVCPEAGGPIQFPGFIGELICPAYHELCSVDSVSTSGQCPNSCNSNGDCVGGICHCFIGFSGDDCNERSCPSNCSGNGKCLKNGICECANGYTGIDCSTAVCDKQCSLHGGVCDNGVCEFRCSDYAGYTCRNISDVLDSLAFCKDVLINDAAGQHCVPSESSVLQQVEEVVVMPNYHRLYPSGARKILNMFRGRNCDKAAKRLACWISIQKCEKDGDNRLRVCHSACQAYNLACGASLDCSDQTNFSEEGDWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GP73</accession>
    <name>A0AAD5GP73_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Angio-associated migratory cell protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_031510</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006576">
        <property type="protein sequence ID" value="KAI7748224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GP73"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051179">
        <property type="entry name" value="WD_repeat_multifunction"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19857:SF8">
        <property type="entry name" value="ANGIO-ASSOCIATED MIGRATORY CELL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19857">
        <property type="entry name" value="MITOCHONDRIAL DIVISION PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="93"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="127"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="169"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="211"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="253"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7748224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="290" mass="31472" checksum="E5AED21C6AEFC913" modified="2024-05-29" version="1">MSANNDDEDNGEVFLNEDDDILEEIYNMDQQADLPKDADEAFNDEADDDDDSIHIFTGHMGELYTVACTRQLVATGAGDDKGFLWKIGQPDWAFELHGKTICTGSDDASLRVWNPSTGKNNHVVKGHYYHTEGLTCLTITADSSLVLTGSKDASVYIVNIATGKVVSSLASHSESVECIGLSAARSPWAATGSMDQKLIIWDLQHSLARCTCEHEEGVTCLTWLGVTRFVATGCVDGKVRIWDSLSGECVKTLRGHTDAIQSLATSSDGNHLVSVSLDGTARVFEINEFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GR79</accession>
    <name>A0AAD5GR79_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_021661</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006167">
        <property type="protein sequence ID" value="KAI7750324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GR79"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26312:SF168">
        <property type="entry name" value="OS06G0606700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26312">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7750324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="178" mass="19569" checksum="4A7911CB1A65659B" modified="2024-05-29" version="1" fragment="single">SSNKIVRSSSESDLASFLPSKRRNSLSGGASSLLSSVSVEEDVEAEEIECKGSLVLANYAKYLKEMYVQGDVLKAEEYCSRAILANPNDGTALSMYAELIWETHKDASRARSYFDQAVKASPDDCYVMASYARFLWDADEDEEEDEQEGSNMNLAAQCNYQNASQQFPIVPMAAASQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GRF2</accession>
    <name>A0AAD5GRF2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">4-coumarate--CoA ligase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_030907</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006391">
        <property type="protein sequence ID" value="KAI7749263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GRF2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016405">
        <property type="term" value="F:CoA-ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24096:SF362">
        <property type="entry name" value="4-COUMARATE--COA LIGASE-LIKE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24096">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="3">
        <location>
            <begin position="76"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="4">
        <location>
            <begin position="451"/>
            <end position="506"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7749263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="524" mass="56547" checksum="B66561E2A6CB5DB9" modified="2024-05-29" version="1">MPTSNSSTNPNNGFCSKTLTFHSLRPPSPLPSPSSSLSVTTFIFSLLQSYTTTTTTTATSSSPPSFIDSTPTSNLTIPYSTLPSLITNLSLSLRHLSLSNHHSALIISSNSSYVPVLYLSLFSIGVVVSPINPASSSAEISNVIRLCKPVVAFVTVDVVEKVKQTGFTNRIVVIESDEFVSMMSGVSNEEMSVGVSQLDTAAILYSSGGTTGKIKGVKLTHRNLISTIAGAMAGHQTRSSPAVYLSTVPYFHVYGFCLCLRTVALGDSLVSIGRFDMRLMLRSIVEFKVTHLPLAPPVVVALVDESNKDLVNGTNWRSVETVLCGGAPLTLAVINKFKRRFPNVSLVQAYGLTETTGGVARIAGPYESKVVGTVGRLISNCEAKIVDPNTGAALPPMSRGELWVRGPFVMKGYVDDKEVIDTMVDSDGWLRTGDLCYFDNEGFLFVVDRYPDELAGQVPMGFVVTRKGSTINETQVKDYVAKQVAPYKKLRRVCFIDSIPKNATGKILRKELIKMALSGITSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GRG0</accession>
    <name>A0AAD5GRG0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Branched-chain-amino-acid aminotransferase</fullName>
            <ecNumber evidence="9">2.6.1.42</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_018081</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-isoleucine + 2-oxoglutarate = (S)-3-methyl-2-oxopentanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24801"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:35146"/>
            <dbReference type="ChEBI" id="CHEBI:58045"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:18321"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:17865"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-valine + 2-oxoglutarate = 3-methyl-2-oxobutanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24813"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57762"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.42" evidence="9"/>
    <dbReference type="EMBL" id="JAMZMK010005699">
        <property type="protein sequence ID" value="KAI7752395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GRG0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004084">
        <property type="term" value="F:branched-chain-amino-acid transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009082">
        <property type="term" value="P:branched-chain amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01557">
        <property type="entry name" value="BCAT_beta_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.10.10:FF:000003">
        <property type="entry name" value="Branched-chain-amino-acid aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.10:FF:000003">
        <property type="entry name" value="Branched-chain-amino-acid aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.10.10">
        <property type="entry name" value="D-amino Acid Aminotransferase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001544">
        <property type="entry name" value="Aminotrans_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018300">
        <property type="entry name" value="Aminotrans_IV_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036038">
        <property type="entry name" value="Aminotransferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005786">
        <property type="entry name" value="B_amino_transII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043132">
        <property type="entry name" value="BCAT-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043131">
        <property type="entry name" value="BCAT-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033939">
        <property type="entry name" value="BCAT_family"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01123">
        <property type="entry name" value="ilvE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009897">
        <property type="entry name" value="PRK13357.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42825">
        <property type="entry name" value="AMINO ACID AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42825:SF29">
        <property type="entry name" value="BRANCHED-CHAIN-AMINO-ACID AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01063">
        <property type="entry name" value="Aminotran_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006468">
        <property type="entry name" value="BCAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56752">
        <property type="entry name" value="D-aminoacid aminotransferase-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00770">
        <property type="entry name" value="AA_TRANSFER_CLASS_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="3 9" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="9" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="5 8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="6">
        <location>
            <position position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR006468-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7752395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="369" mass="41002" checksum="5F253061678443A5" modified="2024-05-29" version="1">MIRQSPFLRRLIQISSSKASALRESEPMVRYEEYADIDWNNLGFGLTRTDYMYKAKCTLGDNFEQGQVTDYGNVELSPAAGVLNYGQGIFEGTKAFRGEDGRILLFRPDQNAIRMQIGAERMCMQSPSIQQFVDAVKQTTIANKRWIPPRGKGSLYLRPLLIGTGPTLGLSPAPEYTFLVYASPVGNYFKEGTAPLNLYVNNEFHRATRGGAGGVKSITNYAPVIKAMLKAKEQGYSDVLYLDSIHKKYIEEVSSCNIFLVKGNVISTPATVGTILQGITRKSIIDIARDLGYKVEERLVAIDELMEADEVFTTGTAVSVACVGSVTYNDQRVDYKTGDESVSQKLYKTLVGIQLGKIEDKYNWVVEIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GRH0</accession>
    <name>A0AAD5GRH0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_023658</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006388">
        <property type="protein sequence ID" value="KAI7749323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GRH0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34189">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34189:SF4">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7749323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="138" mass="15218" checksum="95D8F6EA8E268E31" modified="2024-05-29" version="1">MFRSVSTSKVSDDTYYSIYNHSPTSKAVSPALRALALEANELEESSSLLKKDKIGRTRFSEKSVHVIPLILLLCALLLWLSSNPEINMSVKNDIITGEIKRKTVDRGATIQIEVSHADADFGISSHEEGKKLETSIGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GT41</accession>
    <name>A0AAD5GT41_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">oligopeptidase A</fullName>
            <ecNumber evidence="8">3.4.24.70</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_005111</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>Hydrolysis of oligopeptides, with broad specificity. Gly or Ala commonly occur as P1 or P1' residues, but more distant residues are also important, as is shown by the fact that Z-Gly-Pro-Gly-|-Gly-Pro-Ala is cleaved, but not Z-(Gly)(5).</text>
            <dbReference type="EC" id="3.4.24.70"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="9">Binds 1 zinc ion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the peptidase M3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.70" evidence="8"/>
    <dbReference type="EMBL" id="JAMZMK010004265">
        <property type="protein sequence ID" value="KAI7754165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GT41"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006518">
        <property type="term" value="P:peptide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06456">
        <property type="entry name" value="M3A_DCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000009">
        <property type="entry name" value="Oligopeptidase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1370.40:FF:000006">
        <property type="entry name" value="Organellar oligopeptidase A, chloroplastic/mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1370.40:FF:000009">
        <property type="entry name" value="Zincin-like metalloproteases family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1370.10">
        <property type="entry name" value="Neurolysin, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034005">
        <property type="entry name" value="M3A_DCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024077">
        <property type="entry name" value="Neurolysin/TOP_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045666">
        <property type="entry name" value="OpdA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045090">
        <property type="entry name" value="Pept_M3A_M3B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001567">
        <property type="entry name" value="Pept_M3A_M3B_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11804:SF83">
        <property type="entry name" value="LD37516P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11804">
        <property type="entry name" value="PROTEASE M3 THIMET OLIGOPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01432">
        <property type="entry name" value="Peptidase_M3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19310">
        <property type="entry name" value="TOP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 9" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2 9" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Oligopeptidase A N-terminal" evidence="11">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M3A/M3B catalytic" evidence="10">
        <location>
            <begin position="140"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00026100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF19310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7754165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="606" mass="68477" checksum="479A5FFB6BF442DD" modified="2024-05-29" version="1" fragment="single">QPERITFGLKLDQSKPIYNAFKAMKESPDWATLSDARKRIVESSIKDAVLSGISLEDDEREEFNKIQQELAKLSKTFGENVLDATKKFEKLITDKKEIEGLPATALGLAAQIATSKGHENATAENGPWMITLDGPSFMSVMQHAKNRALREEIYRAYVTRASSGDLDNTPVIEQILKLRLAKAKLLGYNNYAELSMATKMATVDKAEELLEKLRSASWNAAVQDMEELKQFARSQGAPEADELAHWDISFWSERLRESKYEINEEELRPYFSLPKVMDGLFNLVKMLFDIDVVPADGLAPVWNSDVQFYCIKDSTGAPISYFYFDPYSRPSEKRGGAWMGEVFARTRVFSNDKTSVRLPVAHMVCNQMPPVGDKPSLMTFREVETVFHEFGHALQHMLTKQDEGLVAGIRGIEWDAVELPSQFMENWCYHRDTLMSIAKHYETGECLPEDIYQKLLAARTFRAGTMSLRQLKFATLDLELHSKYVPGGPESIYDVDQRVGKRTQLLPPLPEDRFLCGFSHIFAGGYAAGYYSYKWAEVLSADAFSAFEDAGLNDDKALRDTGYRFRDTVLALGGGKDPLEVFIEFRGREPSPEPLLRHNGLLQTTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GTC8</accession>
    <name>A0AAD5GTC8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein NRT1/ PTR FAMILY 1.2-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_016971</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Phosphate:H(+) symporter (TC 2.A.1.9) family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Proton-dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005657">
        <property type="protein sequence ID" value="KAI7752559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GTC8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006857">
        <property type="term" value="P:oligopeptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17416">
        <property type="entry name" value="MFS_NPF1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000109">
        <property type="entry name" value="POT_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018456">
        <property type="entry name" value="PTR2_symporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11654">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00854">
        <property type="entry name" value="PTR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01022">
        <property type="entry name" value="PTR2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="66"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="91"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="184"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="211"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="363"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="405"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="490"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="535"/>
            <end position="557"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7752559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="582" mass="65356" checksum="1E11ED73FEBE7A1D" modified="2024-05-29" version="1">MEIHSTQESEMKQERSSRIRKGGFRTMPFILANESFEKVASYGLLPNMIIYLMSDYHMSLAKGTNVILIWNAATNFLPIAGAFIADSFLGRFFTIFLGSIVSLMGMILLWLTTMIRDAKPATCDLRTFLTCKSPTPIQYTLLYSAFAFMSIGAGGVRPCSLAFGADQIDSKDNSKRERMLESFFGWYYSTAIMAVMIALTAIVYIQDHHGWRVGFGIPVILMFFSTFLFVVAYPLYYKIKVEKSLFTSFCQVILVAWKNRRLELPNSVNGSWYNKNDANITKPTDNLRFLNKACTINKAQNMTNAIAKDPWTVCTVDQVEELKTLIKVIPLWSSCIMLSVANSQTTFPVIQARAMDRHITSRFEIPAGSFSFFTIVTIMVWVILYDRVILPLLSKIQGKPAHISVKLRMGAGLVCSIIAMVISGIVEHVRKNKAIEAGFLNNPQGVINMSAMWLIPQHVLSGLADALNIIGQNEFFYSEFPKSMSSIAMALYMLGSAFASLLASLLLSIVNDLTKGHGKERWISTNINKGHYDKYYWLLGVMSCINFLYYVVCSWSYRSCVVKTAVEGDKTSDSGDEHETKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GTS0</accession>
    <name>A0AAD5GTS0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA (cytosine-5-)-methyltransferase</fullName>
            <ecNumber evidence="2">2.1.1.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_008123</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.37" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010002257">
        <property type="protein sequence ID" value="KAI7754930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003886">
        <property type="term" value="F:DNA (cytosine-5-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006346">
        <property type="term" value="P:DNA methylation-dependent constitutive heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044027">
        <property type="term" value="P:negative regulation of gene expression via chromosomal CpG island methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04708">
        <property type="entry name" value="BAH_plantDCM_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000128">
        <property type="entry name" value="DNA (cytosine-5)-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.120.10:FF:000002">
        <property type="entry name" value="DNA (cytosine-5)-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.120.10:FF:000004">
        <property type="entry name" value="DNA (cytosine-5)-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.490">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.120.10">
        <property type="entry name" value="DNA Methylase, subunit A, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001025">
        <property type="entry name" value="BAH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043151">
        <property type="entry name" value="BAH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050390">
        <property type="entry name" value="C5-Methyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018117">
        <property type="entry name" value="C5_DNA_meth_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001525">
        <property type="entry name" value="C5_MeTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022702">
        <property type="entry name" value="Cytosine_MeTrfase1_RFD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10629">
        <property type="entry name" value="CYTOSINE-SPECIFIC METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10629:SF52">
        <property type="entry name" value="DNA (CYTOSINE-5)-METHYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01426">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00145">
        <property type="entry name" value="DNA_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12047">
        <property type="entry name" value="DNMT1-RFD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037404">
        <property type="entry name" value="DNMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00105">
        <property type="entry name" value="C5METTRFRASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00439">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51038">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00094">
        <property type="entry name" value="C5_MTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51679">
        <property type="entry name" value="SAM_MT_C5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 10" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="BAH" evidence="12">
        <location>
            <begin position="760"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="domain" description="BAH" evidence="12">
        <location>
            <begin position="933"/>
            <end position="1073"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="683"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="683"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="693"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="723"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="active site" evidence="9 10">
        <location>
            <position position="1225"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="1511"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR037404-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7754930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="1511" mass="169803" checksum="D0425017F577EA0B" modified="2024-05-29" version="1" fragment="single">MGTARLLESEEGGAVKPSEMKKKGKQAKSTSEVSDITAEVKPKAATKLKQKRGRSDSSENAPAPRKMPKRAASCTSFKSKPVRLSDKHETIENKKVQVVEEEIAALSLIPGPDDPRPNRRLTDFIFHDADGNPQPVEMLEVDDLFISGLILPFEKVEKEKEAGVRCEGFGRIEDWAISGYEDGSPVIWVSTDLADYDCVKPAGSYKKLFGLFYEKARSCVEVYKRLSKSSGGDPDLSLDELLASLVRSMSGSKNFPHGASIRDLIISWGAFIYEQLVGLDDAPNSTDKLFVELPALAALRDESKKKEEFVGMNVSSNGSMNAPLKIGDGEKTEKVNMSKGKHVADEDEDTKLARVLQENENWQSMKQNKRPRHATTSSSKLYIQINEDEIANDYPLPAYYKTSIQETDEYLLFDDFFDTVDPDYLPKSMLHNWSLYNCDSRLISLELLPMKPCADIDVTVFGSGIMTTDDGSGFCFDNEPITASGSSSAAQNEDGIPIYLSAIKEWKIEFGSSMVIISIRTDMAWYRLGKPSKQYGPWYQTVLKTARLAIAIITLLKEQTRASKLSEFDKNHPAYISSNPVNVERYVVVHGQIILQQFAEFPDVTIRKCAFISGLESKMVERHHTKWTVKKKTIETKQANLNPRAAMGPVVSKRKAMPATTTRFINRIWGEFYSNYSPEEVKEGDSIDVKKDEEEEDEPEENEQEDCEEPEEENNTLPVQVPEKPHSAVKEKKPCGSKTGIKWVGKAVGNMANGNALYKEAVIRGEVVGLGSSVLVEDTIYYVEYLFEDSDSRKFVHGRLMLRGCETVLGNIANEREVFLTNDCVDFELDDVIQPVVVQIRTIAWGHEHRKANANFDKMDRAKAEDRKSKGLPVEYYCKSLYWPQRGAFFSLKTDKMGLGNGICYSCKITDDEKKKEIFEVNESKTGFTYMATEYRVGDFIYVGPHHFDSDERGNETHKGGRNVGLKAYVVCQLIQIESPKSLKRVDPESVSVQVRRFYRPEDLSSDKAYRSDIQEVYYSEETLKLSVSALEGKCEVKRKTDLSSLDSTYIVDHVFFCERLYDPDKGSLKQLPVNVKFSSPKEDLVDEAAIRKRKGKCKEGESDIEMTDHQQSSTVNNRLTTLDIFAGCGGLSEGLQKAGASVTKWAIEYEEPAGDAFRLNHPDALAFVHNCNVILRAIMTACGDTDDCISTSEAEEMAAKLEEDEIKNLPRPGQVDFINGGPPCQGFSGMNRFNQSTWSKVQCEMILAFLSFAEYFRPKYFLLENVRNFVSFNKGQTFRLTLASLLEMGYQVRFGILEAGAFGVSQSRKRAFIWAASPEEILPEWPEPMHVFASPELRVSLNSNTQYAAVRSTASGAPFRAITVRDTIGELPEVGNGASATTIEYKTEPISWFQKRIRGDASVLTDHISKEMNELNVIRCQRIPKRPGADWRDLPEEKVKLSTGQMVDLIPWCLPNTAKRHNQWKGLFGRLDWEGNFPTSITDPQPMGKVGMCFHPDQDRILTVRECARS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GU97</accession>
    <name>A0AAD5GU97_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glucan endo-1,3-beta-D-glucosidase</fullName>
            <ecNumber evidence="3">3.2.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_009249</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of (1-&gt;3)-beta-D-glucosidic linkages in (1-&gt;3)-beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.39"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the glycosyl hydrolase 17 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.39" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010005766">
        <property type="protein sequence ID" value="KAI7752068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GU97"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042973">
        <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000005">
        <property type="entry name" value="Glucan endo-1,3-beta-glucosidase 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000490">
        <property type="entry name" value="Glyco_hydro_17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044965">
        <property type="entry name" value="Glyco_hydro_17_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32227">
        <property type="entry name" value="GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00332">
        <property type="entry name" value="Glyco_hydro_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7752068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="316" mass="35045" checksum="D31250F5C6182644" modified="2024-05-29" version="1">MKASKIMNTRIFDADHEVLKAFKGSGIEITVGLGNEFLTDISVNEDRAMNWIKENVEPFLPGTRIVGIAVGNEILGGGNQELWEVLLPAAKNVYNALDRLHLADKVQVSSPHSAAVFATSYPPSAGAFKESVMPYMKPLLDFFSQIKSPFYINTYPFLAYISDPDHIDLNYALFKKNPGIYDQKTNLHYDNMFEAQIDASYAALEKAGFENMEVIVSETGWASHGDPNEAAATVSNARTYNFNLRKRLLKKKGTPYRPKRVVKAYVFAMFNENLKPGPTSERNFGLFKADGSVSYNIGFTGLVPSSAKSLIAMKVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GUA6</accession>
    <name>A0AAD5GUA6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Tonoplast dicarboxylate transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_023109</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005760">
        <property type="protein sequence ID" value="KAI7752098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GUA6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015140">
        <property type="term" value="F:malate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001898">
        <property type="entry name" value="SLC13A/DASS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10283">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 13 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10283:SF82">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 13 MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00939">
        <property type="entry name" value="Na_sulph_symp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="32"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="59"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="112"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="151"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="204"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="249"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="379"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="411"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="454"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="493"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="577"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="611"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="657"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="738"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="775"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="814"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="865"/>
            <end position="886"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7752098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="892" mass="96097" checksum="18FC7B42A9AFF18D" modified="2024-05-29" version="1">MDDENSNSSSSPLLPLHGESSSNIPTTLKSLFTIKNLFILSGPLLCTLICVCVNNKLDVSSRNMLGVLAWVFAWWLTEAVPMPITSMSPLFLFPLFGIAAADDVARSYMDDVISLVLGSFILALAVEHYNIHTRLALNITLVFCRDPVNPQLLLLGICGTTAFVSMWMHNTATTIMMMPVATGILQRLPSSSSGAESSSKFCKAVVLGVIYSAAVGGMSTLTGTGVNLILVGMWKTYFPQADPLSFSTWFFFGFPMALLLFFALWVILCLAYCPKAGSAHTLSAYLDTSHIKRELDLLGPMAFAEKMVLGVFSSAAGTETETENKLVTKKVILIDTDPDFAMDDENSNSSSSPLLPLHGESSSNIIPTTLKSLLTIKNLFILSGPLLCTLICVCVNNKLDVSSRNMLGVLAWVFAWWLTEAVPMPITSMSPLFLFPLFGIAAADDVARSYMDDVISLVLGSFILALAVEHYNIHTRLALNITLVFCRDPVNPQLLLLGICGTTAFVSMWMHNTATTIMMMPVATGILQRLPSSSSSGGESSSNFCKAVVLGILQRLPSSSSSGGESSSNFCNATTSVGGMSTLTGTGVNLILVGMWKSYFPQADPLSFSTWFFFGFPMALLLFFALWVILCLAYCPKAGSAHTLSAYLDISHIKRELDLLGPMAFAEKMVLGVFSALIVLWMTRSITDDIPGWGTLFNDRVGDGTVSVMMATLLFIIPNKKQQGEKLMDWNKCKKLPWNIVLLLGAGLAIAEGVRTSGVADVLSRTLDFLGQTPYLAIAPAVCVISGFMTELITSNNATTTILLPLLIEIAKNINVHPLLLMVPGAIGAQFAFLLPTATPSNVIGFTTGHIDVKDMVKAGLPLKFAGTLALSVLMPSLGALVFVSLKTGQQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GUJ4</accession>
    <name>A0AAD5GUJ4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glucan endo-1,3-beta-D-glucosidase</fullName>
            <ecNumber evidence="3">3.2.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_025938</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of (1-&gt;3)-beta-D-glucosidic linkages in (1-&gt;3)-beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.39"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the glycosyl hydrolase 17 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.39" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010005704">
        <property type="protein sequence ID" value="KAI7752353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GUJ4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042973">
        <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000005">
        <property type="entry name" value="Glucan endo-1,3-beta-glucosidase 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000490">
        <property type="entry name" value="Glyco_hydro_17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044965">
        <property type="entry name" value="Glyco_hydro_17_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32227">
        <property type="entry name" value="GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00332">
        <property type="entry name" value="Glyco_hydro_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041941926" description="glucan endo-1,3-beta-D-glucosidase" evidence="8">
        <location>
            <begin position="30"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7752353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="408" mass="44310" checksum="EB7DB5D81DF53A90" modified="2024-05-29" version="1">MDHFTTHLLHISAALLLLIFLCSTLSVSSIGINYGQIANNLPSPDKVVPLVKCIGADKVKLYDADPKVLKAFANTDIEFVVGIGNEYLSKLQDPDEAQSWVKSNVQRYLPATKITSIAVGNEVLTFNDTSLSDCLLPAMQNIHTALVNLKLDSQVSVTTAHSLAVLQTSYPPSFGAFRDDLKDCISPILDFLSKTCSPFLINAYPFFAYKGNSKEVSLDFVLFQPNAGIVDSGNNLHYDNMLFAQIDAVYAALDTLGYKNLPVQISETGWPSKGDEDEPGASPENAKKYNGNLFKVVNQTKGTPAKPDYDLNIFVFALFNENLKPGPTSERNYGLFKPDGSPAYNLGFSGITAGGNSGRNTSGTAPAPPYTLLPENPANGYMSISSAGRFEWKNSIRIIVMLMLIVVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GXD2</accession>
    <name>A0AAD5GXD2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UspA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_025983</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010001697">
        <property type="protein sequence ID" value="KAI7755113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GXD2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23659">
        <property type="entry name" value="USP_At3g01520-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000206">
        <property type="entry name" value="Universal stress protein family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46100:SF12">
        <property type="entry name" value="ADENINE NUCLEOTIDE ALPHA HYDROLASES-LIKE SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46100">
        <property type="entry name" value="IMP2'P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="1">
        <location>
            <begin position="5"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7755113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="159" mass="17786" checksum="72BFAEB160918883" modified="2024-05-29" version="1">MVKDRKVGVAMDFSKSSKAALQWAIDNLADKGDTFYIIHIKSSSSDESRNKLWETDGSPLIPLVEFREPEVMAKYGLKTDIDVLDILDTGARQKEIQVVTKLYWGDAREKLVEAIEDMKLDTLVMGSRGLSTLKRIILGSVTNYLVTHAPCPVTIVKDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GXP6</accession>
    <name>A0AAD5GXP6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DUF642 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_004655</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010001109">
        <property type="protein sequence ID" value="KAI7755458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GXP6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000031">
        <property type="entry name" value="DUF642 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006946">
        <property type="entry name" value="DGR2-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052437">
        <property type="entry name" value="Pectin_Meth_Modulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31265:SF2">
        <property type="entry name" value="F17A17.37 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31265">
        <property type="entry name" value="OS02G0527500 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04862">
        <property type="entry name" value="DUF642"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0134">Cell wall</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041934652" description="DUF642 domain-containing protein" evidence="6">
        <location>
            <begin position="21"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="DUF642" evidence="7">
        <location>
            <begin position="27"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="DUF642" evidence="7">
        <location>
            <begin position="195"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7755458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="366" mass="39644" checksum="032F96F6372AA233" modified="2024-05-29" version="1">MTHFLSLLFTLTIISTYVFAAQPPLEGLLPNGNFEEPPKPTDMKKTVLIGKHALPKWEITGLVEYIHGGPQPGGMYFPVAHGVHAVRLGNEATVSQTLSVKPGSLYAVTFGASRTCAQEEVLRVSVPPQSGDLPLQTLYSSDGGDVYAYGFRANSSSVKLTFHNPGVQEDPQCGPLIDAVAIKELLPPRPTRFNLVKNGGFEEGPHRLFNSSNGVLLPPRQEDITSPLPGWIIESLKAVKFIDNKHFNVPSGVAAIELVAGRESAIAQIIRTVPNKMYMLSFVIGDAKNGCHGDMMVEAFAAKETLKAPFKSVGQGKSKTVSLKFKAISARTRVTFYSSYYHTRADDFGSLCGPVIDEVKVSSVRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GYX1</accession>
    <name>A0AAD5GYX1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">OTU domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_002085</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C85 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000029">
        <property type="protein sequence ID" value="KAI7758094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GYX1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22797">
        <property type="entry name" value="OTU_plant_OTU5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.80:FF:000014">
        <property type="entry name" value="Cysteine proteinases superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003323">
        <property type="entry name" value="OTU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050704">
        <property type="entry name" value="Peptidase_C85-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12419:SF10">
        <property type="entry name" value="DEUBIQUITINASE OTUD6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12419">
        <property type="entry name" value="OTU DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02338">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50802">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OTU" evidence="4">
        <location>
            <begin position="169"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="112"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="19"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="121"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="131"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7758094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="317" mass="35659" checksum="0E830DF48DD844FF" modified="2024-05-29" version="1">MENTQDLEENQTKEVIVEKETREEMLSRHRKEITQLQNKEIALKKAAAKGSKAEQKAKKKQVEEQVSQLTTELKEKHATELASVGYANVNATGKDKASDIDILAKAIAGVSVTNQTEHSKPTKSAKRKEKRAQQEAAREQRIQEEQSNIVSDRMVESESLEKKLEPLGLVINEIKPDGHCLYRAVEDQLKVKGSTFYTFQELRKMVADYMRQNSSDFLPFFLSEAVTDGDYDDPAVEKRFESYCNEIESTAAWGGQLELGALTHCLKKHIMIFSGSFPDVEMGKEYKAGESLGSSIILSYHKHAFGLGEHYNSVVPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GZ11</accession>
    <name>A0AAD5GZ11_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Endopeptidase S2P</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_018751</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000016">
        <property type="protein sequence ID" value="KAI7758229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GZ11"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031293">
        <property type="term" value="P:membrane protein intracellular domain proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905897">
        <property type="term" value="P:regulation of response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001193">
        <property type="entry name" value="MBTPS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008915">
        <property type="entry name" value="Peptidase_M50"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13325:SF3">
        <property type="entry name" value="MEMBRANE-BOUND TRANSCRIPTION FACTOR SITE-2 PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13325">
        <property type="entry name" value="PROTEASE M50 MEMBRANE-BOUND TRANSCRIPTION FACTOR SITE 2 PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02163">
        <property type="entry name" value="Peptidase_M50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01000">
        <property type="entry name" value="SREBPS2PTASE"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="70"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="161"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="201"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="513"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M50" evidence="8">
        <location>
            <begin position="140"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00032658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7758229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="539" mass="60031" checksum="34E6BB9433FF58D1" modified="2024-05-29" version="1">MAEPNNIQLQPGRRPIRRPNTATPSHTLLPLRRSPHLSNSISCWYCDFKTSVFNEPIFHFGRKHFRWLRIWFSIGMGFSLTTLIGVTLIMLLESAKALNLYNGIPWLNNILDGSILGFYSSVFKYSISVADIGYMCVSSIISVAVHELGHALAATSEGIPIEYTALFLAVMFPGALVAFNYESLQIIPRAATLRIYCAGVWHNAAFCVVCALTVFLLPFILYPLYSHGESLMVLDVSAESPLYGYLSPGYLITKLDGNPVHTSKEWMETVYLLDKQISQNTNHYISNKGYCVPNLAIAESKNAEIVDNQFKCPNDLTLFTTISCVDSSTANDISSNETDHQLMMENVYCFPAKGLLGEKKCGNGWNNAVKNDTNCLCTEDESCSTPIHVPGVSWVEITFSRPYSSDCTQAGIEIGSSYNKSWSEESKCGGSFVFIGDMMSMSRSIWLTEYQPRWPYGFAYFPDLVEKLLVNSFHVSLMLALLNSLPVYYLDGESILEVALCFFGSLTPRIRDAVLRSSLVAGTLICCLLFSRILLSVMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5H039</accession>
    <name>A0AAD5H039_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_001687</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HD-ZIP homeobox family. Class II subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000067">
        <property type="protein sequence ID" value="KAI7757753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5H039"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000577">
        <property type="entry name" value="Homeobox-leucine zipper protein 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050762">
        <property type="entry name" value="HD-ZIP_Homeobox_LZ_Class_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003106">
        <property type="entry name" value="Leu_zip_homeo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45714">
        <property type="entry name" value="HOMEOBOX-LEUCINE ZIPPER PROTEIN HAT14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45714:SF39">
        <property type="entry name" value="HOMEOBOX-LEUCINE ZIPPER PROTEIN HAT14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02183">
        <property type="entry name" value="HALZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00340">
        <property type="entry name" value="HALZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 8 9" id="KW-0371">Homeobox</keyword>
    <keyword evidence="7 8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="11">
        <location>
            <begin position="210"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="8">
        <location>
            <begin position="212"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="122"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="329"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="18"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="138"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7757753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="353" mass="39482" checksum="20DB6D93A426A72C" modified="2024-05-29" version="1">MELALSLGDTPKPISIGDESRSQQQKNKELGFCMVLGSSSQNIKDHQQKNYEDDDKTNCSSSSSDPRPLQLDLRPFSPSLHQSSPPPPPSHRSFPWLSQILVIGEPSSQTKLGKGLDVKPLNAIYDPDNGEEPAPVQSSPNDSNSDTSSFQMDFSSIFKSSTGSSRFQSAKPDMEFVRGNMDFFADLSERDHRVSSRRVGYGGTSDDDENGLGRKKLRLTKEQSAFLEDSFKEHNTLNPKQKLILANQLNLRPRQVEVWFQNRRARTKLKQTEVDCEYLKTCCETLTEENKRLQKELQELRALKTSQPFYMQLPATTLTMCPSCERVVTTTSTNTTPPNTTTAAPQQPANGCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BMP1</accession>
    <name>A0AAD5BMP1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_020199</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the iron-sulfur cluster assembly SufBD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011749">
        <property type="protein sequence ID" value="KAI7726120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BMP1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055346">
        <property type="entry name" value="Fe-S_cluster_assembly_SufBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010231">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000825">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufBD_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037284">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045595">
        <property type="entry name" value="SufBD_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008773">
        <property type="entry name" value="PRK11814.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01980">
        <property type="entry name" value="sufB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30508">
        <property type="entry name" value="FES CLUSTER ASSEMBLY PROTEIN SUF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30508:SF1">
        <property type="entry name" value="UPF0051 PROTEIN ABCI8, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01458">
        <property type="entry name" value="SUFBD_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19295">
        <property type="entry name" value="SufBD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101960">
        <property type="entry name" value="Stabilizer of iron transporter SufD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SUF system FeS cluster assembly SufBD N-terminal" evidence="4">
        <location>
            <begin position="209"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="SUF system FeS cluster assembly SufBD core" evidence="3">
        <location>
            <begin position="281"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="16"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="54"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="25"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="54"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="64"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00043967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF19295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7726120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="552" mass="61961" checksum="4391D28E11DCDBB0" modified="2024-05-29" version="1">MASLVTNSLLPTFSSQPISDNPLLSKGSPSSFDFSSKSKSRNSRVLKLRADSKTIQSDTITQGNNNNNPSLSSNNDDPLQKFLKRDYKWGFNQEIDSFTIPKGLSEETIRLISSRKNEPDWMLEFRLNSYYKFCQMTEPKWSDNTYAKIDFQDICYYSEPKKKPTLNSLDEADPELIKYFDKLGITLNEKKRLANVAVDAVLDSVSIATTHRKTLEKSGVIFCSISEAIREYPDLVRKYLGKVVSTDDNYYAALNSAVFSDGSFVYIPKDTKCPMQISTYFRINAMETGQFERTLIVADDRSFVEYLEGCTAPSYDTNQLHAAVVELHCNEGAEIKYSTVQNWYAGDENGRGGIYNFVTKRGLCAGRKSKISWTQVETGSAITWKYPSVVLQGDDSVGEFYSVALTNNYQQADTGTKMIHKGKNTKSRIISKGISAGNSRNCYRGLVQVQSRAENARNSSQCDSMLIGDKAAANTYPYIQAKNPSARIEHEASTSKIGEDQLFYFQQRGIDYERAMAAMISGFCRDVFNELPDEFGAEVNQLMSLKLENSVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BMQ7</accession>
    <name>A0AAD5BMQ7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Annexin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_008571</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="domain">
        <text evidence="6">A pair of annexin repeats may form one binding site for calcium and phospholipid.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the annexin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011726">
        <property type="protein sequence ID" value="KAI7726227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BMQ7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001786">
        <property type="term" value="F:phosphatidylserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009409">
        <property type="term" value="P:response to cold"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009651">
        <property type="term" value="P:response to salt stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009414">
        <property type="term" value="P:response to water deprivation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000001">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000002">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.10">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001464">
        <property type="entry name" value="Annexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018502">
        <property type="entry name" value="Annexin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018252">
        <property type="entry name" value="Annexin_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037104">
        <property type="entry name" value="Annexin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10502">
        <property type="entry name" value="ANNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10502:SF102">
        <property type="entry name" value="ANNEXIN B11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00191">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00196">
        <property type="entry name" value="ANNEXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00335">
        <property type="entry name" value="ANX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47874">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00223">
        <property type="entry name" value="ANNEXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51897">
        <property type="entry name" value="ANNEXIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0041">Annexin</keyword>
    <keyword evidence="3 6" id="KW-0106">Calcium</keyword>
    <keyword evidence="5 6" id="KW-0111">Calcium/phospholipid-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7726227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="150" mass="16851" checksum="F30E55978D1915CD" modified="2024-05-29" version="1">MAAKDAKALYKAGEKKLGTDEKVFVKIFSERSGAHLLAVNTCYHDMYGGSLKKVIKKETSGLFELALLTILQCAENPAKYFAKVLYKSMQGLGTNDTTLIRVIVTRTEIDMQYIKAEYHKKYKKSLNDAVHSETSGHYRTFLLSLLGPNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BR68</accession>
    <name>A0AAD5BR68_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">HMG box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_013159</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HMGB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011326">
        <property type="protein sequence ID" value="KAI7727769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BR68"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd22005">
        <property type="entry name" value="HMG-box_AtHMGB1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031061">
        <property type="entry name" value="HMGB_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46261:SF32">
        <property type="entry name" value="HIGH MOBILITY GROUP B PROTEIN 3-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46261">
        <property type="entry name" value="HIGH MOBILITY GROUP B PROTEIN 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMG box" evidence="7">
        <location>
            <begin position="35"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="5">
        <location>
            <begin position="35"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="13"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="102"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7727769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="126" mass="14123" checksum="EA1146AD3EEC21C7" modified="2024-05-29" version="1">MRGPKNTVIIAEKKLHPNTTKKTKNKASSKNSVAPKRPPSAFFIFMEQFRRDNKENFPENKSASAIAKEGGVIWKSMTESKAPYVAKAARKKAEYEIAIKQHNDNLNDDAMEISSSSSINTHEDVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BSR3</accession>
    <name>A0AAD5BSR3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_025555</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011142">
        <property type="protein sequence ID" value="KAI7728797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BSR3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7728797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="61" mass="7501" checksum="99227D0C20598150" modified="2024-05-29" version="1" fragment="single">QDPVVWDLAGCRWHYLRSGDVYGLYIQDLVVRFVKADRLIKMRKRPWFWMFFFCCNILKIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BVG2</accession>
    <name>A0AAD5BVG2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_021857</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011090">
        <property type="protein sequence ID" value="KAI7729034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BVG2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7729034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="287" mass="33410" checksum="19F17CE14A9BA129" modified="2024-05-29" version="1" fragment="single">REIGERERSRDERQIRGDDRLAVVCAQLIGPHLSYTTRQAFLPSLYSPDDYRRTESVQAPHPYHYMYQHLSSGINYDSFVWEQMDFTLYARLTVMDSLLGILLHKGEGRMRMLGRLTWWSKTIFHRRQCSTISATVAGGGLRWRAEAAVVMATTDLSNYICVHINNRSWTIVCYKTYHGGPLISRWLLELFKNPPILNVHQTTGNRANTVRIRGFLQVPSSSNFNFVQRDYFRALGYSIYNFMLVDLFRHTVLPEDQVDEPRRYTRPLHISTRITTFATKTSHGVIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BWF3</accession>
    <name>A0AAD5BWF3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Peptidase S10, serine carboxypeptidase, Alpha/Beta hydrolase fold protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_022667</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010595">
        <property type="protein sequence ID" value="KAI7730892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BWF3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019748">
        <property type="term" value="P:secondary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12670:FF:000002">
        <property type="entry name" value="Carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12670">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033124">
        <property type="entry name" value="Ser_caboxypep_his_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF382">
        <property type="entry name" value="PEPTIDASE S10, SERINE CARBOXYPEPTIDASE, ALPHA_BETA HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00560">
        <property type="entry name" value="CARBOXYPEPT_SER_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042051611" description="Peptidase S10, serine carboxypeptidase, Alpha/Beta hydrolase fold protein" evidence="6">
        <location>
            <begin position="26"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7730892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="456" mass="51463" checksum="5E6F0769D1712FAD" modified="2024-05-29" version="1">MQNPKYMGLICMLVIVVSIANLVASQTIVKTLPGYPGPLPFKLETGYIGVGEDEAVQLFYYFVESEGKPEEDPLIIWLAGGPGCGTLRAFFFEIGPMQIQYGKYMDNVPALQLDPNSWTKVANVIYLDAPTLTGYSYTKTPEAVHSSDTASASQTAEFMRKFVRDHPKFLKNPMYITGISYSGIVIPMITEELYKGNDEGLQPTVNIQKTRKDCNGDYAEADDNNMQCMSDIDEVNKRVGDINIQQILDPDCDPATNLVRGGNPVVAKGNRKTLQANPIRMLIPSSRSTLVTDTTFCRGEYYDYATLWANDENVMKALNVRQGTVKEWLLCNLDMKYNYGKRSMPQYEFNIRSSVVYHQKLTQRNCRALIFSGDHDMMVPHVGTRNWINTLNLTITDSDWEPYYVNGQNGGYKTTYARQNYSLVFATVKGAGHTAPEFKPAECFEMVKRWFAHRPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BXS3</accession>
    <name>A0AAD5BXS3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">uridine/cytidine kinase</fullName>
            <ecNumber evidence="5">2.7.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_030260</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Pyrimidine metabolism; CTP biosynthesis via salvage pathway; CTP from cytidine: step 1/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; UMP biosynthesis via salvage pathway; UMP from uridine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the C-terminal section; belongs to the UPRTase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the uridine kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.48" evidence="5"/>
    <dbReference type="EMBL" id="JAMZMK010010516">
        <property type="protein sequence ID" value="KAI7731189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BXS3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004849">
        <property type="term" value="F:uridine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02023">
        <property type="entry name" value="UMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000015">
        <property type="entry name" value="Uridine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006083">
        <property type="entry name" value="PRK/URK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000764">
        <property type="entry name" value="Uridine_kinase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001097">
        <property type="entry name" value="PRK00129.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10285">
        <property type="entry name" value="URIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00485">
        <property type="entry name" value="PRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14681">
        <property type="entry name" value="UPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00988">
        <property type="entry name" value="URIDINKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribulokinase/uridine kinase" evidence="12">
        <location>
            <begin position="51"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="13">
        <location>
            <begin position="210"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF14681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7731189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="414" mass="46530" checksum="46FC4A4AEC66605D" modified="2024-05-29" version="1">MGSTPVEDMIEASSGAHFSGFHIDGMQPLNSLAGQLATSLSENVHKQPFIIDAFDNEKLLSTVEMLRHGKTVDIPKYDFRSYKNSISRRVNPSDVIILEGILIFHDPRIREIMNMKIFVDTDADVRLARRIRRDTGEKGRDIGMVLDQYSKFVKPAFDDFILPTKKYADIIIPRGGDNHVAIDLIVQHIRTKLGQHDLCKIYPNLYVIHSTFQIRGMHTLIRDAQTTKHDFVFYADRLIRLVVEHGLGHLPFTEKQVVTPTGSVYTGVDFCKRLCGVSVIRSGESMENALRACCKGIKIGKILIHREGDNGQQLIYEKLPQDISDRHVLLLDPILGTGNSAVQAISLLLKKGVPEANIIFLNLISAPQGVHVVCKRFPRIKIVTSEIETGLNQEFRVLPGMGEFGDRYFGTDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BYG9</accession>
    <name>A0AAD5BYG9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transcription factor bHLH143-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_030189</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010344">
        <property type="protein sequence ID" value="KAI7732001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BYG9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037546">
        <property type="entry name" value="SAC51-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36066">
        <property type="entry name" value="TRANSCRIPTION FACTOR BHLH145"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36066:SF2">
        <property type="entry name" value="TRANSCRIPTION FACTOR BHLH145"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="222"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="222"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7732001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="370" mass="40884" checksum="6FF4D781573EA32C" modified="2024-05-29" version="1" fragment="single">GKIKVVMVCQSAGQTRFRALKHLNGTAGRPTIILKLIACFQPLQHSQFSVIYTRPRNPSSPDQQTDWRSLNPNFSSTQFGLGPRVMFSSFGNMVSPPVYREPCNQFYGLPRYRQGIGPTVKSSAKEVNEKLPKASPVVARETNGVQKKFIVFDQSGDQTTLMYSLGNGPSSARYQFPSLNRPKPQFGSHTVCVDKLINENDGNSSQSEMREDTEELNALLSSDDDYDDEDEDNDDEMSTGHSPCLMAGIRGQKREVDSGEEEEVASCEVPLKRCKKDDASSLVNTLEDTACSGKSGVNCSGNGVLEEEYDNISCGQKKLRIEKMKETINVLQNLLPGDRSGKNAIMVIDEAIHYFTSLKVQVKALGLDSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BYH0</accession>
    <name>A0AAD5BYH0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">MIR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_032216</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010364">
        <property type="protein sequence ID" value="KAI7731852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BYH0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008725">
        <property type="term" value="F:DNA-3-methyladenine glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23294">
        <property type="entry name" value="beta-trefoil_MIR_AtSDF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000068">
        <property type="entry name" value="Stromal cell-derived factor 2-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.30">
        <property type="entry name" value="Hypothetical protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005019">
        <property type="entry name" value="Adenine_glyco"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036300">
        <property type="entry name" value="MIR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016093">
        <property type="entry name" value="MIR_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46809:SF2">
        <property type="entry name" value="GH21273P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46809">
        <property type="entry name" value="STROMAL CELL-DERIVED FACTOR 2-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03352">
        <property type="entry name" value="Adenine_glyco"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02815">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00472">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="DNA-glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82109">
        <property type="entry name" value="MIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50919">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="MIR" evidence="3">
        <location>
            <begin position="152"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="3">
        <location>
            <begin position="214"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="3">
        <location>
            <begin position="272"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7731852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="338" mass="37655" checksum="283A6CA5C84616B7" modified="2024-05-29" version="1">MLFFMMKNGECRKLFELLSLSTALAEHTWPSILNKRHLFREVFHGFDPVTVSKLNDKKISAPGNPATSLLSEVKLRGIIENARQVYVGPTKDISDNSGQKGRSDNGNGLGLTSVIDELRLSGFCMTSCFFSLAILLFLTLHSDTVYAATSEVIEITYGSVIKLMHERTKFRLHSHDVPYGSGSGQQSVTSFPNVDDANSYWIVKPVPDTSVKSGDNIKTGSVIRLQHLRTRKWLHSHLHASPISGNLEVSCFGDDGNSDTGDYWRIEIVGSGKTWRQDQRIRLLHVDTSGYLHSHDKKFNRIASGQQEVCGVREKRADNVWLAAEGVYLPTNESKAAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BYV5</accession>
    <name>A0AAD5BYV5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_006109</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SBNO family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010280">
        <property type="protein sequence ID" value="KAI7732226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BYV5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000342">
        <property type="entry name" value="Protein strawberry notch homolog 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026937">
        <property type="entry name" value="SBNO_Helicase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026741">
        <property type="entry name" value="SNO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039187">
        <property type="entry name" value="SNO_AAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057024">
        <property type="entry name" value="Znr_FGT1_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057025">
        <property type="entry name" value="Znr_FGT1_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12706:SF13">
        <property type="entry name" value="PROTEIN FORGETTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12706">
        <property type="entry name" value="STRAWBERRY NOTCH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13872">
        <property type="entry name" value="AAA_34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13871">
        <property type="entry name" value="Helicase_C_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23547">
        <property type="entry name" value="Zn_ribbon_FGT1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23548">
        <property type="entry name" value="Zn_ribbon_FGT1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="9">
        <location>
            <begin position="658"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="578"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="579"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1008"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7732226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="1008" mass="111274" checksum="3D5B0F6CACF69C57" modified="2024-05-29" version="1" fragment="single">MTQPPVSPPLPLPQPPPPQPRPPPFPNSAVGCQVRCAGCRMILTVAPGLTEFVCPTCQLPQMLPPELLRPPSQVPAHGIDPTKIQLPCANCKAILNVPHGLSRFACPQCKMELAVDLSRIKQFYPPPVEEVNEVAIEVEREEDGGGLVGETFTDYRPQKLSIGPPHPDPIVETSSLSAVQPPEPTYDLKTKDYLETSKALSCLQIETLVYASQRHLQHLENGSRAGFFLGDGAGVGKGRTIAGLIWENWQHGRRKALWISVGSDLKFDARRDLDDVGATSIEVHALNKLSYSKLDSKSVGIREGVVFLTYSSLIASSEKGRSRLQQLVQWCGSDYDGLIVFDECHKAKNLVPEAGGQPTRTGEAVLELQARLPEARVVYCSATGASEPRNLGYMVRLGLWGAGTSFEDFRQFLGALDKGGVGALELVAMDMKARGMYVCRTLSYKGVEFEVLEVPLEAKMMDIYKKAAEFWAELRVELLSASAFLGDDKPNSSQLWRLYWASHQRFFRYVCMSAKVPAVVSLSKQALMENKCVVIGLQSTGEARTEDAITKYGNDLDDFISGPRELLLKFVEENYPLPEKPEPLPEEDSVKELQRKRHSANPDVSFRGRVRKAAKWKPASDEDSAEESECAVTNGISNLLFNQVADSGNESVESDDEFQICNICNSEAERKKLLQCSCCGQLIHPACVVPPETEAISSDWSCHSCKEKTEEYLQARHIYLAELLKRYEAATERKSKILDIIRNFDLPNNPLDDIIDQLGGPGNVAEITGRRGMLVRASNGKGVTYQARNTKDITMEMVNMHEKQLFMDGKKLVAIISEAGSAGVSLQADRRALNQKRRVHVTLELPWSADRAIQQFGRTHRSNQASAPEYRLLFTNLGGERRFASIVAKRLESLGALTQGDRRAGPSLSAYNYDSSYGKKALLMLYRGIMEQDSLPVVPPNCSSDNPATIRDFIEKGKAALVSVGIVRDTVVGNGKDSGKFSGRIVDSDMHDVGRFLNRLLGLPPEIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C1K0</accession>
    <name>A0AAD5C1K0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">K-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_006197</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009924">
        <property type="protein sequence ID" value="KAI7733713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C1K0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002487">
        <property type="entry name" value="TF_Kbox"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01486">
        <property type="entry name" value="K-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51297">
        <property type="entry name" value="K_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="K-box" evidence="1">
        <location>
            <begin position="22"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7733713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="117" mass="13964" checksum="5A8C4ECA2703C336" modified="2024-05-29" version="1" fragment="single">MKNYREHVMANRSFTDEIQQENPHMQHNVTLIQKKIEQLEVSTRKLLGQSLGSCALDELLKLENKVESSLTTLRERKMHLFMEHIQELKEKERLLMKDNARLWQHYATLCKKRECGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C343</accession>
    <name>A0AAD5C343_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_011540</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009778">
        <property type="protein sequence ID" value="KAI7734227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C343"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35099:SF10">
        <property type="entry name" value="BZIP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35099">
        <property type="entry name" value="OS02G0182700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="29"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="118"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="54"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7734227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="196" mass="21739" checksum="4252BF7CD902D04F" modified="2024-05-29" version="1">MNTDDNWLQSAFDDVTLVAQVILNLRHSSPSSLPKTWTTRQRRSRPPPPATVKKPSSTRDSPTTPLSLSGGAATTSVSGGGVEETSRLILDGSDALRSKVIRANETTPTKRPRRKKTLAELKEDEIALLKERKQLKRQLASLQATCQKQRKENESLKKMENEMKSQQVLAVSNCTEEQKNDSFVIPDLNIPAGEEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C3D6</accession>
    <name>A0AAD5C3D6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glycosyl transferase, family 14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_006009</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009743">
        <property type="protein sequence ID" value="KAI7734372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C3D6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044174">
        <property type="entry name" value="BC10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003406">
        <property type="entry name" value="Glyco_trans_14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31042">
        <property type="entry name" value="CORE-2/I-BRANCHING BETA-1,6-N-ACETYLGLUCOSAMINYLTRANSFERASE FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31042:SF77">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02485">
        <property type="entry name" value="Branch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7734372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="224" mass="26115" checksum="7277250199BC6D60" modified="2024-05-29" version="1" fragment="single">MPPKVSITKEMPMMHNMSDEDLVKSVMEISKVTTTNTPYKVAFMFLTPGPLPLAPLWELFFKGHNGLFTIYVHPHPSYNDTIPHDSIFYGTRITSQPVYWGDISMVDAERRLLANALLDPSNQRFMLLSDSCIPLFNFTTTYNYLVNSNLSYISSYDEKRKSGRGRYNPQMSPNITIHDWRKGSQWFEVNRDLALEIVTDKKYYTLFKEYCHPPCYNDEHYLPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C4B5</accession>
    <name>A0AAD5C4B5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_021694</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009908">
        <property type="protein sequence ID" value="KAI7733776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C4B5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080030">
        <property type="term" value="F:methyl indole-3-acetate esterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080032">
        <property type="term" value="F:methyl jasmonate esterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080031">
        <property type="term" value="F:methyl salicylate esterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009694">
        <property type="term" value="P:jasmonic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009696">
        <property type="term" value="P:salicylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045889">
        <property type="entry name" value="MES/HNL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10992:SF943">
        <property type="entry name" value="METHYLESTERASE 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10992">
        <property type="entry name" value="METHYLESTERASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="11"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7733776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="218" mass="24412" checksum="02F0B40448C4A0C4" modified="2024-05-29" version="1">MPTTTESRKHLVLVHGLCHGAWCWYKVMTNLRSAGYHVSAVDLGGSGMHPNQLDEIITFSDYIHPLFQFLESLSADERVVLVGHSFGGLAISLAMERFPHIVSVGVFITAYMPNCKDTPALQMAQDLTLAKMLIRPSHFFLEDMSQDSLLTSDKYGSVSRVYVVCEGDRVVNEEFQRFVIEDSPPNEVKSFPGAGHMIMLSIPKDISLYLQEIATRYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-10-08" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C5L0</accession>
    <name>A0AAD5C5L0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_000543</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009435">
        <property type="protein sequence ID" value="KAI7735781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000051">
        <property type="entry name" value="Receptor-like serine/threonine-protein kinase ALE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000146">
        <property type="entry name" value="receptor-like serine/threonine-protein kinase ALE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057597">
        <property type="entry name" value="ALE2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47989:SF45">
        <property type="entry name" value="OS01G0709500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47989">
        <property type="entry name" value="OS01G0750732 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23180">
        <property type="entry name" value="ALE2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042108055" description="Protein kinase domain-containing protein" evidence="8">
        <location>
            <begin position="22"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="365"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="79"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="280"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="36"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="91"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="287"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="312"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="393"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7735781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="743" mass="82385" checksum="5CE8D2E7827A4E4B" modified="2024-05-29" version="1">MGFQVVXXLITFFILFATFDSLTFRSAEKVKLSTDHGVSQAQSPNNDQYLAPDHYENHLPSKHHATKWIKRSFHRHHHHESSKLNHVAPTPSYQVQPPTYMPQGSSGPAPSPVDLLSQFGPQISPSSSSLTKNTASQPSPVFMLPSPPPNEDCTTVTCTQPLTYTPAGSPCACVWPIAIKLRLMISPYAFFSLVSELAHEIATSLSLNVSQARFTGANAVGNQLDTTIVLINLVPLKQNFDPDTTFLIYQKFWNKQLAINQTLFGAYEVIDVKYPGLPPSPSSIPSNTDVTIDQSDSNKGIPSKPFGVDIPRNGRDDKRLGAGDSLIVGSRKSSETMSFSSSLAAYAGTAKIYSLSEIQRATCNFDSTRILGEGGFGVVYSGVLQDDRKVAVKVLKRDDRQGSREFLAEIEMLSRLHHRNLVKLFGICTDDHFRCLVYELVPNGSVESHLHGADKIAPLNWCPRMKIALGAARGLAYLHEDSSPRVIHRDFKSSNILLENDFTPKVSDFGLARTAMDGHMHVSTHVMGTFGYLAPEYAMTGHLLVKSDVYSYGVVLLELLTGRKPVDLLQPPGQENLVTWARPLLTNKEALESIIDPSIINSNTPFDSILKVAAIASMCVQPEVSHRPFMGEVVQALKLVCNEFDEIREMDSISCSRDEYLDFETFDQSEIHHETSGYDSKIGLSARIENIGSLESESFRRQFNSAPLKIGRKKQFWKRLRSLSRGSMSEHEHDHDHGFSSNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C5U2</accession>
    <name>A0AAD5C5U2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_011859</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009467">
        <property type="protein sequence ID" value="KAI7735570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C5U2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7735570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="80" mass="9485" checksum="60B6FCA0D45E598A" modified="2024-05-29" version="1" fragment="single">IEIEEVNNEIMTMLIFLQLKYHLEFQGLGICFYVVPKKVQQLQIILKLKHVEQLYIANDLRNLPVAIYRPHVSSIKETLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C5U7</accession>
    <name>A0AAD5C5U7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_033035</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009488">
        <property type="protein sequence ID" value="KAI7735434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C5U7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7735434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="65" mass="7050" checksum="0227C8F40B3A1D8A" modified="2024-05-29" version="1" fragment="single">MVALVKVRAATIDARLLTVGGLVLITIGNKTAGCYKGQKVGVLTNYTLKNLLMDVRDKLLLKHIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C8A2</accession>
    <name>A0AAD5C8A2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_018025</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009251">
        <property type="protein sequence ID" value="KAI7736550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C8A2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01959">
        <property type="entry name" value="nsLTP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.110.10">
        <property type="entry name" value="Plant lipid-transfer and hydrophobic proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036312">
        <property type="entry name" value="Bifun_inhib/LTP/seed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016140">
        <property type="entry name" value="Bifunc_inhib/LTP/seed_store"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033872">
        <property type="entry name" value="nsLTP2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33214">
        <property type="entry name" value="BIFUNCTIONAL INHIBITOR/LIPID-TRANSFER PROTEIN/SEED STORAGE 2S ALBUMIN SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33214:SF44">
        <property type="entry name" value="NON-SPECIFIC LIPID TRANSFER PROTEIN GPI-ANCHORED 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00234">
        <property type="entry name" value="Tryp_alpha_amyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00499">
        <property type="entry name" value="AAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47699">
        <property type="entry name" value="Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042242230" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein" evidence="3">
        <location>
            <begin position="24"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical" evidence="4">
        <location>
            <begin position="31"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7736550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="96" mass="10003" checksum="754FBC082E2532C0" modified="2024-05-29" version="1">MRSATCMAAYALLVLVVILAGSAVNTTAVTCSVSELSPCLPSFTSSVPPSSQCCSKLKEQKPCLCGYIKNPSLKQYITSPNAKKVSSTCGVPIPKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C8I0</accession>
    <name>A0AAD5C8I0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">acetolactate synthase</fullName>
            <ecNumber evidence="6">2.2.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">M8C21_015820</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">AA19_3_7</strain>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>2 pyruvate + H(+) = (2S)-2-acetolactate + CO2</text>
            <dbReference type="Rhea" id="RHEA:25249"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:58476"/>
            <dbReference type="EC" id="2.2.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 1/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 16">Belongs to the TPP enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.6" evidence="6"/>
    <dbReference type="EMBL" id="JAMZMK010009187">
        <property type="protein sequence ID" value="KAI7736814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C8I0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005948">
        <property type="term" value="C:acetolactate synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003984">
        <property type="term" value="F:acetolactate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:L-valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02015">
        <property type="entry name" value="TPP_AHAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07035">
        <property type="entry name" value="TPP_PYR_POX_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1220:FF:000008">
        <property type="entry name" value="Acetolactate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000053">
        <property type="entry name" value="Acetolactate synthase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039368">
        <property type="entry name" value="AHAS_TPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012000">
        <property type="entry name" value="Thiamin_PyroP_enz_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000399">
        <property type="entry name" value="TPP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045229">
        <property type="entry name" value="TPP_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968:SF13">
        <property type="entry name" value="ACETOLACTATE SYNTHASE CATALYTIC SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968">
        <property type="entry name" value="THIAMINE PYROPHOSPHATE ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00205">
        <property type="entry name" value="TPP_enzyme_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00187">
        <property type="entry name" value="TPP_ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="14" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0274">FAD</keyword>
    <keyword evidence="8" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13 16" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme N-terminal TPP-binding" evidence="19">
        <location>
            <begin position="26"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme central" evidence="17">
        <location>
            <begin position="124"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme TPP-binding" evidence="18">
        <location>
            <begin position="306"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="20">
        <location>
            <position position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU362132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAI7736814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="492" mass="53868" checksum="1FB574B7C9FA2783" modified="2024-05-29" version="1" fragment="single">QPRKGADVLVEALEREGVTDVFAYPATNLVSGLADALLDSVPMVAITGQVPRRMIGTDAFQETPIVEVTRSITKHNYLVLDVEDIPRVVREAFYLASSGRPGPVLIDVPKDIQQQLVVPKWDEPMRLPGGGCLNSADELRRFVELTGIPVASTLMGLGAYPASSDLSLHMLGMHGTVYANYAVDKSDLLLAFGVRFDDRVTGKLEAFASRAKIVHIDIDSAEIGKNKQPHVSICGDIKVALQGLNEILEEKNSVTNLDFSNWRKELDEQKVKFPLNFKTFGEAIPPQYAIQVLDELTGGNAIISTGVGQHQMWAAQFYKYNKPRQWLTSGGLGAMGFGLPAAIGAAVARPDAVVVDIDGDGSFIMNVQELATIRVENLPVKILLLNNQHLGMVVQWEDRFYKANRAHTYLGNPSKESEIFPNMLKFAEACDIPAARVTRKADLRAAIQKMLDTPGPYLLDVIVPHQEHVLPMIPAGGGFMDVITEGDGRTKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C8T6</accession>
    <name>A0AAD5C8T6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">B box-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_004276</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009212">
        <property type="protein sequence ID" value="KAI7736688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C8T6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009640">
        <property type="term" value="P:photomorphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19821">
        <property type="entry name" value="Bbox1_BBX-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000589">
        <property type="entry name" value="B-box zinc finger protein 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051979">
        <property type="entry name" value="B-box_zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049808">
        <property type="entry name" value="CONSTANS-like_Bbox1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31832">
        <property type="entry name" value="B-BOX ZINC FINGER PROTEIN 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31832:SF68">
        <property type="entry name" value="B-BOX ZINC FINGER PROTEIN 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="B box-type" evidence="11">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="11">
        <location>
            <begin position="52"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="113"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="228"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="114"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="233"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7736688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="295" mass="31794" checksum="07D52BA3D7097028" modified="2024-05-29" version="1">MKIQCNVCESSEAAVLCCADEAALCWSCDEKVHAANKLASKHQRVPLSTSTSQMPKCDICQEAAGYFFCLEDRALLCRNCDVAIHTVNSLVSSHQRFLLTGVKVGLEAADLGASSSTGKSTSGEKILEPEKPHSVPANQFNKVMPVEAGDFVPSKMPYTGGSSADSIQWQFDDFLALTDYNQNYNYLDNDSSKADSGKLGESDGSPILRAMDIELDDEECLGQVPDASWAVPQMSSPPTASGLSWPKDRPRYQHPPGSDTYVPDVCYLPMPNFYGSRQNAGASQSGVAGINECKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C9W6</accession>
    <name>A0AAD5C9W6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_027451</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008993">
        <property type="protein sequence ID" value="KAI7737580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C9W6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055336">
        <property type="entry name" value="At4g00755-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39741">
        <property type="entry name" value="F-BOX DOMAIN CONTAINING PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39741:SF14">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="14"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7737580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="315" mass="35768" checksum="37CFE6894E011058" modified="2024-05-29" version="1" fragment="single">MGTHVDFLEGLDPDMALKILTCLDDATDLVRASVVSRYWQKTVISNGLSKQLCVRTFPQLASVIRVVEPSHGNLGETERDHRVYASLFRALTAFPLANCIANPVSASSTDRYPEESIMNTVAPINTITLLGSYWSSIGTDDPDTPETLIYRLTANFCVITDIYLHPWQDPYTSCLCSSGFVRFQMGHPKSWKELNYDFIEAQECADDKFIWTYTSPVFPVAQENRLQRFKLPDPVLCIGGYLQIELLGRINKAIDDKYYLWRAWLNQSCTTSPLAWGIYKLQNCIHIEQAHGVGVECEWVDYDEPEMDGKDEIDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CA86</accession>
    <name>A0AAD5CA86_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SBP-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_016757</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008848">
        <property type="protein sequence ID" value="KAI7738228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CA86"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1100.10">
        <property type="entry name" value="Transcription factor, SBP-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044817">
        <property type="entry name" value="SBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004333">
        <property type="entry name" value="SBP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036893">
        <property type="entry name" value="SBP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31251">
        <property type="entry name" value="SQUAMOSA PROMOTER-BINDING-LIKE PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31251:SF227">
        <property type="entry name" value="TRANSCRIPTION FACTOR, SBP-BOX-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03110">
        <property type="entry name" value="SBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103612">
        <property type="entry name" value="SBT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51141">
        <property type="entry name" value="ZF_SBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SBP-type" evidence="6">
        <location>
            <begin position="7"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="73"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7738228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="219" mass="24449" checksum="FF8D7142CDB60708" modified="2024-05-29" version="1">MADVNNAVMCLVDGCAADLSDCRKYYQRHKVCENHSKSPQVSINGQILRFCQQCSRFHSLGEFDEGKRSCRKRLDGHNRRRRKPRPQQPFPLNHGTNMLQFADQQVQVHPHLHPTLDFNNPLWSGVVDAPAWVDHSSYAPSLLSSSVAPPQASVSTLSHVMYPPSESGSVTLAASPLDQGPSYGGSENDYEVGWGGVTDMDPHYHESSDPQTLPFYWQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CB50</accession>
    <name>A0AAD5CB50_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_030793</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 32 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008750">
        <property type="protein sequence ID" value="KAI7738691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CB50"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18624">
        <property type="entry name" value="GH32_Fruct1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.560:FF:000002">
        <property type="entry name" value="Beta-fructofuranosidase, insoluble isoenzyme CWINV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.560">
        <property type="entry name" value="Exo-inulinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.115.10.20">
        <property type="entry name" value="Glycosyl hydrolase domain, family 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050551">
        <property type="entry name" value="Fructan_Metab_Enzymes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001362">
        <property type="entry name" value="Glyco_hydro_32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013189">
        <property type="entry name" value="Glyco_hydro_32_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013148">
        <property type="entry name" value="Glyco_hydro_32_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023296">
        <property type="entry name" value="Glyco_hydro_beta-prop_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31953">
        <property type="entry name" value="BETA-FRUCTOFURANOSIDASE, INSOLUBLE ISOENZYME CWINV1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08244">
        <property type="entry name" value="Glyco_hydro_32C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00251">
        <property type="entry name" value="Glyco_hydro_32N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00640">
        <property type="entry name" value="Glyco_32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75005">
        <property type="entry name" value="Arabinanase/levansucrase/invertase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyl hydrolase family 32 N-terminal" evidence="5">
        <location>
            <begin position="13"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 32 C-terminal" evidence="6">
        <location>
            <begin position="321"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7738691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="523" mass="58404" checksum="10BBE20215F39269" modified="2024-05-29" version="1">MLICNQCSCSILGPLFHMGWYHLFYQYNPYAPVWGNMSWGHSVSKDMINWYELPVALVPTEWYDIEGVLSGSTTVLPNGQIFALYTGNANDFSQLQCKAVPVNVSDPLLVEWVKYNDNPILYTPPGIGLKDYRDPSTVWTGPDGKHRMIMGSKRGNTGMVLVYHTTDYTNYELLDEPMHSVPNTDMWECVDFYPVSLTNDSALDMAAYGSGIKHVIKESWEGHGMDFYSIGTYDAINDKWTPDNPELDVGIGLRCDYGRFFASKSIYDPLKKRRITWAYVGESDSPDEDLSRGWATIYNVGRTIVLDRKTGTHLLHWPVEEIESLRYNGREFKEIKLEPGSIVPLDIGTATQLDIVATFKVDQDALNATSGTDDKFGCTTSSGAAERGSLGPFGFAVLADGTLSELTPVYFYIAKKADGGVSTHFCTDKLRSSLDYDKERVVYGSTVPVLDDEELTMRILVDHSIVEGFAQGGRTVITSRVYPTKAIYEGAKLFVFNNATGTSVKASLKIWQMASAQIHQYPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CB67</accession>
    <name>A0AAD5CB67_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Sm protein E</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_004922</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the snRNP Sm proteins family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008917">
        <property type="protein sequence ID" value="KAI7737960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CB67"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027078">
        <property type="entry name" value="snRNP-E"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11193">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Sm" evidence="10">
        <location>
            <begin position="23"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7737960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="62" mass="7310" checksum="6BD547C543615E7E" modified="2024-05-29" version="1">MATKVQRIMTQPINLIFRFLQSGFDEYMNLVLDEAEEVSIKKKTRKALGESKKEHLHEDDRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CCV4</accession>
    <name>A0AAD5CCV4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Auxin response factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_032589</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Auxin response factors (ARFs) are transcriptional factors that bind specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs).</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimers and heterodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the ARF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008673">
        <property type="protein sequence ID" value="KAI7739109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CCV4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009734">
        <property type="term" value="P:auxin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10017">
        <property type="entry name" value="B3_DNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.1040:FF:000001">
        <property type="entry name" value="Auxin response factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.330.10:FF:000001">
        <property type="entry name" value="Auxin response factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000047">
        <property type="entry name" value="Auxin response factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.330.10">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010525">
        <property type="entry name" value="ARF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044835">
        <property type="entry name" value="ARF_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033389">
        <property type="entry name" value="AUX/IAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003340">
        <property type="entry name" value="B3_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015300">
        <property type="entry name" value="DNA-bd_pseudobarrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053793">
        <property type="entry name" value="PB1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31384:SF9">
        <property type="entry name" value="AUXIN RESPONSE FACTOR 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31384">
        <property type="entry name" value="AUXIN RESPONSE FACTOR 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06507">
        <property type="entry name" value="ARF_AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02309">
        <property type="entry name" value="AUX_IAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02362">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01019">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101936">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50863">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51745">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0927">Auxin signaling pathway</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TF-B3" evidence="11">
        <location>
            <begin position="128"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="PB1" evidence="12">
        <location>
            <begin position="887"/>
            <end position="971"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="485"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="541"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="600"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="787"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="489"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="542"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="600"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="620"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="796"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7739109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="990" mass="110347" checksum="615BF828E4938B30" modified="2024-05-29" version="1" fragment="single">MKMAVTADNSQSEGGEKKNIINPELWQTCAGPLVDLPAAGTHVVYFPQGHSEQVAASMKKDVDAQIPNYSNLQSKLLCIVHSVTLHADPETDEVYAQMTLQPVPSFDKEALLRSDLSMKINKPQTQFFCKTLTASDTSTHGGFSVPRRAAEKIFPPLDFSMQPPVQELVARDLHDTQWTFRHIYRGQPKRHLLTTGWSLFVSGKKLFAGDAVLFIRDENQQLLLGIRRANRQPANLSSSVLSSDSMHIGILAAAAHAAANNSPFTVFYNPRASPSEFVIPLAKYYKAVCSNQISLGMRFRMMFETEESGTRRYMGTVTGISDLDPVNWKNSQWRNLQVGWDESTGGERRTRVSVWEIEPVTAPFFICPTPPFYRPKRPRQPGMLDDESADIDNLFKRTMPWLGEDMSTKDSGLSLVQWMNMQQSSSLSNSVQPSSLNPLSGSLLQNYGAAQMLTQNNLQFNSQRSTMPLDPLQKLPQAASMSPLASMMQPHQQLTDPSQQLNRQNSINQSQPQNHIQSQFHPQSLLQQQSSMHNNNPYRSLQQQQQVTTVSQSQSQSQSQSQQNSLLLPQYSDQQISQQLQMPNNQIPLQLLQKLRQQQQSLFGQQSAPQQDHEKPLVNVGQSQSQSFTRSLSTSQTTDMPLETHNTPLSQQMSRNNSQANLRFSHLSPQQKIHPTNQTWTAGIGGQSGITDDVPSCSTSPSTNNCPTMMSSMINSRSYASTTIGGEEIMINNGIESQHVSENVNFVKDSPSVNTSKTQNQGFLTPLTYLNNNINGAQMDFLDSSSSATSVSIPQSDHHLQSDPLRDTIQDDPRTEIPTGASMDNNFGMPSMILDPLTTKGSNGGMISQSFGVPDMDFNPIDSTLNDSNLLDSDVWAAPPQQFQRKRTYTKVYKRGAVGRSIDISAYSGYEELKQDLARRFGIEGQLEDRQRVGWKLVYVDHETDVLLVGDDPWEEFVSCVRCIKILSPQEVQQMSLDGDFGGNNVVQNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CD05</accession>
    <name>A0AAD5CD05_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_018890</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008662">
        <property type="protein sequence ID" value="KAI7739184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CD05"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04051">
        <property type="entry name" value="C2_SRC2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044750">
        <property type="entry name" value="C2_SRC2/BAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32246:SF22">
        <property type="entry name" value="C2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32246">
        <property type="entry name" value="INGRESSION PROTEIN FIC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="1">
        <location>
            <begin position="1"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7739184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="161" mass="18306" checksum="3C4B7C95D5CB70BA" modified="2024-05-29" version="1">MECRILELTINSANDLREVRRMFKMKVYAKVFIGGNQEMEKRTPVDRHGQANPAWNYTMKYSISESWLQHHGTMLVIKLYCKRKLGDRYIGEVHQPLKQLYDYAYPLGGSAVVRFPVQMGSAESQGQLCFSYRFGEKVAIDKLMLAESIASFLVTGSGGAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CDD5</accession>
    <name>A0AAD5CDD5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Rho termination factor N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_017348</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008607">
        <property type="protein sequence ID" value="KAI7739537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CDD5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="435"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="442"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="551"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7739537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="551" mass="62167" checksum="F35ED482E45A3214" modified="2024-05-29" version="1" fragment="single">SLLPLKTHFILYLNPTNPYISPTPIIHTILPSNSQVILSPGTMDGLWDPPHYQDVIEETAINEKSCIEVLEILTSKADADIAEHEELLMDLLSQLACTDEQFSDMFVRCLTAKIDFLDSSIRKLKDDDEEFLSTRREPAESIYEILKSLFHLYSQNKDLQGCLFMFSLQNQKCAENNGTASTSCCPVPADNQKKQVGSSSKANQMEKIKTVNFTSVQKQTIIPTKVKVEEENTDYPHTSPVRSLYWGSKNKAGREVHKFQIKSPIIMKKDILQNRTPRCSSICPKKYSETQGKQKHQLSIVKSQPYDESMSLATVPYDESISLPTVPCSESMSLATVPYDESISLSTVPCSESMSLATVPYDESMSLATVPYVESMSLETVSYDENMSLATVPYGESFEEINAMSLTTIPYLESHEEANVKQLIIQELDFPPGYQPQHHQKQSISRSRNLSSTRTYTRRKKPNSPLRTNSNSHFALAVASTFDVEMNYTVNDLRAIAKERNFKRFYKLRKAELAQLLGIKLSEGRGKQLKKADTQLVSLIKREPEFAAIGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CE98</accession>
    <name>A0AAD5CE98_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">M8C21_011737</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">AA19_3_7</strain>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010008451">
        <property type="protein sequence ID" value="KAI7740372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CE98"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000400">
        <property type="entry name" value="Nuclear pore complex protein NUP107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051420">
        <property type="entry name" value="Ser_Thr_Kinases_DiverseReg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48005">
        <property type="entry name" value="LEUCINE RICH REPEAT KINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48005:SF95">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="1"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="17">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAI7740372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="278" mass="30753" checksum="491920FAF6EE3076" modified="2024-05-29" version="1" fragment="single">SSEEVNALLKWKATLHSENSNTLLPTWTPDLNPNISAPKIVSPCNWYGVSCNEVGNINRLNLSSSGLNGTLDHLSLSSFPNLEYFDLSLNNFSGIIPSEIRNLSQLVLLDFSSNQFTGIIPLEIGELRKLATLYLFQNQLNGSIPQSICQMTFLSRLALNENNLYGSIPTCFGTFGYLAPELAYTMKVTEKCDVYSFGVLVLEIFKGQHPGDIITSLASTTTEAVKLTDLVDHRLRDQLPEIKEVLTSILILAIKCINSNPEIRPTMQEISQKIASIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CEU5</accession>
    <name>A0AAD5CEU5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_013859</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008502">
        <property type="protein sequence ID" value="KAI7740159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CEU5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000129">
        <property type="entry name" value="Leucine-rich repeat receptor-like kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053213">
        <property type="entry name" value="RLP29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48009">
        <property type="entry name" value="LEUCINE-RICH REPEAT (LRR) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48009:SF9">
        <property type="entry name" value="LRR RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE GSO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042170107" evidence="8">
        <location>
            <begin position="25"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7740159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="383" mass="41248" checksum="AF4566E3D7A972E6" modified="2024-05-29" version="1">MNPITFSISTTLSLLLLLTTTTLSTTLTTDITALKSILSSINPTTIPPNSCLSTWNFTSNPCTTPHLTHFLCGLSCTNNHITHLTFDRAGYSGTLSPLVSQLVQLIIIDLSDNKLSGPIPISIFSLPNLQTLNLRSNSFSGTIPPLISALKSIETIDVSHNVISGELPDSLSGLGLTRLDLSFNKIVGRIPKLPSMLIELALRDNLLSGDLLKTSFGELTQLEVVELGGNSLTGTIPGWFFLLPALQQVNLANNGFTGIEVLKPVNSNLIAFDAGYNKITGYLSSNFSNYPMLSSLTLRYNKLHGPIPTGYSQSTTLKRLFLDGNYLNGMPPKEFFTGKSSVSGSLGDNCLKSCPVSSQLCSKSQKPSAICQQAYRGKVKQKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CH95</accession>
    <name>A0AAD5CH95_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">M8C21_019869</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">AA19_3_7</strain>
            <tissue evidence="21">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010008163">
        <property type="protein sequence ID" value="KAI7741592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CH95"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000309">
        <property type="entry name" value="Leucine-rich repeat receptor protein kinase MSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000041">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000400">
        <property type="entry name" value="Nuclear pore complex protein NUP107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051420">
        <property type="entry name" value="Ser_Thr_Kinases_DiverseReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48005">
        <property type="entry name" value="LEUCINE RICH REPEAT KINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48005:SF16">
        <property type="entry name" value="MDIS1-INTERACTING RECEPTOR LIKE KINASE 2-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="14 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="296"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="20">
        <location>
            <begin position="358"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="21">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAI7741592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="627" mass="69884" checksum="CF9207A83B1D9EC8" modified="2024-05-29" version="1" fragment="single">TANDFNISLEAKASLADPHCKRYGIICNEAGSAIAINLYNDNLEGQGDLLGSLDFASFPNLERLSIQNCKLEGSIPEQIGLLSNLTELSLPGNLLTGNLPVSLTNLTRLKYLNLSKNQLTGPIPPSYGSMLSLTVLDMGTNQLNASIPEEIGNLQNLETLNLAHNNFDGPIPSNFGNLSQLKILNISNNFLSGNIPLSIANLVNLKQLDLNNNGLVGSVHGYLSRLSYLDVSSNRLSGNMSFQNPCNLKHLDLSMNQMTGAITSLQVCSDLEYLDLTSNHFIGEEHKRKNELVKHLTIFLPVIVGLCFLVLVCIYCKNRKATEKQIQPITKRHGNVCSVLNYDGRIAYEDFITATRDFNLKYCIGTCSYGRVYKAKLPDGKIFALKILHHFESQQSTLISQAFKNEIQMLTNLRHKNIVRLYGFCSHNKCNFLVYEYIEKGSLYCALTNRELATELDWLKRVNIIKGVAHALAYMHHDCSPPIVHRDISSKNILLNSKLEGLVAHFGAARLLDPDSCKQTAIVGTLGYTAPELGYDVVTEKCDVYSFGVVALETIRAKHPRDPFSSMNNLNRHVTTLENILDRRLPYPTDKLIEKEIMRVYNVALSCILTDPKCRPTMRNVCKELSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CIB7</accession>
    <name>A0AAD5CIB7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TATA box binding protein associated factor (TAF) histone-like fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_000624</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008134">
        <property type="protein sequence ID" value="KAI7741780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CIB7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046695">
        <property type="term" value="C:SLIK (SAGA-like) complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22931">
        <property type="entry name" value="HFD_TAF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08050">
        <property type="entry name" value="TAF6C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000046">
        <property type="entry name" value="transcription initiation factor TFIID subunit 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.770:FF:000001">
        <property type="entry name" value="Transcription initiation factor TFIID subunit 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.770">
        <property type="entry name" value="TAF6, C-terminal HEAT repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037796">
        <property type="entry name" value="TAF6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011442">
        <property type="entry name" value="TAF6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046344">
        <property type="entry name" value="TAF6_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004823">
        <property type="entry name" value="TAF_TATA-bd_Histone-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10221">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10221:SF13">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02969">
        <property type="entry name" value="TAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07571">
        <property type="entry name" value="TAF6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00803">
        <property type="entry name" value="TAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TATA box binding protein associated factor (TAF) histone-like fold" evidence="6">
        <location>
            <begin position="2"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7741780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="339" mass="37989" checksum="9900D524038D3FDE" modified="2024-05-29" version="1" fragment="single">MSIVPKETVEVIAQSIGINNLSPDAALALAPDVEYRMREIIQEGIKCMHHSKRTTLTTDDVDSALGMRNVEPIYGFASGDPLKFKRALGHNDLFYVDDKDVDFKDVIEAPLPKAPLDTSFFCHWLAIEGVQPAIPENAPVEVIAALPETKKSEQKSEELPVDIRLPVKHVLSRELQLYFNKVTELAVSMPDSVLFKEALASLATDSGLHPLVPYFTCFIEDEVSRGLNDFQLLFALMRLVCSLLQNPHIHVEPYLHQLMPPVVTCIVTKRLGNRIADNHWELRDFTAKLAATICKRFGHNYSSLQTRLTKTLLKSFLDRKRTLTQHYGAVQGLAALGPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CJH3</accession>
    <name>A0AAD5CJH3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_002748</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008148">
        <property type="protein sequence ID" value="KAI7741681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CJH3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045167">
        <property type="entry name" value="Hobbit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15678">
        <property type="entry name" value="ANTIGEN MLAA-22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15678:SF11">
        <property type="entry name" value="FMP27, GFWDK DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10344">
        <property type="entry name" value="Hobbit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7741681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="190" mass="20946" checksum="FA30F39DFB6E1479" modified="2024-05-29" version="1" fragment="single">IHLGEPQVTGDAPTIEKSSAHFICEEFGLLFEFGSHSEMGVSVKNIDISLGEISVDLNEGLISKKKKPSGTHVDEPVQPATESDLIAKKEQKKQATLAAITKHTPFFPEKFSLTLPKLDVKFVHKEHHVVMENNITAVQFKSIKSQSIEDESTRIDLQLDFSEIHILKEGGSSIISLQKLAVLSFVCIPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CMW7</accession>
    <name>A0AAD5CMW7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Poly [ADP-ribose] polymerase</fullName>
            <shortName evidence="18">PARP</shortName>
            <ecNumber evidence="18">2.4.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">M8C21_012836</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="25">AA19_3_7</strain>
            <tissue evidence="25">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-aspartyl-[protein] + NAD(+) = 4-O-(ADP-D-ribosyl)-L-aspartyl-[protein] + nicotinamide</text>
            <dbReference type="Rhea" id="RHEA:54424"/>
            <dbReference type="Rhea" id="RHEA-COMP:9867"/>
            <dbReference type="Rhea" id="RHEA-COMP:13832"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29961"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:138102"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>L-glutamyl-[protein] + NAD(+) = 5-O-(ADP-D-ribosyl)-L-glutamyl-[protein] + nicotinamide</text>
            <dbReference type="Rhea" id="RHEA:58224"/>
            <dbReference type="Rhea" id="RHEA-COMP:10208"/>
            <dbReference type="Rhea" id="RHEA-COMP:15089"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29973"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:142540"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>NAD(+) + (ADP-D-ribosyl)n-acceptor = nicotinamide + (ADP-D-ribosyl)n+1-acceptor + H(+).</text>
            <dbReference type="EC" id="2.4.2.30"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the ARTD/PARP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="25">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.-" evidence="18"/>
    <dbReference type="EMBL" id="JAMZMK010007497">
        <property type="protein sequence ID" value="KAI7744562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CMW7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003950">
        <property type="term" value="F:NAD+ poly-ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990404">
        <property type="term" value="F:NAD+-protein mono-ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070212">
        <property type="term" value="P:protein poly-ADP-ribosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01437">
        <property type="entry name" value="parp_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08002">
        <property type="entry name" value="WGR_PARP3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.142.10:FF:000002">
        <property type="entry name" value="Poly [ADP-ribose] polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.140.10:FF:000001">
        <property type="entry name" value="Poly [ADP-ribose] polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.228.10:FF:000002">
        <property type="entry name" value="Poly [ADP-ribose] polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.228.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.142.10">
        <property type="entry name" value="Poly(ADP-ribose) polymerase, regulatory domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.30">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.140.10">
        <property type="entry name" value="WGR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050800">
        <property type="entry name" value="ARTD/PARP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012317">
        <property type="entry name" value="Poly(ADP-ribose)pol_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004102">
        <property type="entry name" value="Poly(ADP-ribose)pol_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036616">
        <property type="entry name" value="Poly(ADP-ribose)pol_reg_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003034">
        <property type="entry name" value="SAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036361">
        <property type="entry name" value="SAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036930">
        <property type="entry name" value="WGR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008893">
        <property type="entry name" value="WGR_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10459">
        <property type="entry name" value="DNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10459:SF60">
        <property type="entry name" value="POLY [ADP-RIBOSE] POLYMERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00644">
        <property type="entry name" value="PARP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02877">
        <property type="entry name" value="PARP_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02037">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05406">
        <property type="entry name" value="WGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00513">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00773">
        <property type="entry name" value="WGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56399">
        <property type="entry name" value="ADP-ribosylation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47587">
        <property type="entry name" value="Domain of poly(ADP-ribose) polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68906">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142921">
        <property type="entry name" value="WGR domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51060">
        <property type="entry name" value="PARP_ALPHA_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51059">
        <property type="entry name" value="PARP_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50800">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51977">
        <property type="entry name" value="WGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0013">ADP-ribosylation</keyword>
    <keyword evidence="19" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 18" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 18" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 18" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SAP" evidence="21">
        <location>
            <begin position="2"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="domain" description="SAP" evidence="21">
        <location>
            <begin position="71"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="WGR" evidence="24">
        <location>
            <begin position="152"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="PARP alpha-helical" evidence="23">
        <location>
            <begin position="279"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="domain" description="PARP catalytic" evidence="22">
        <location>
            <begin position="405"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="19">
        <location>
            <begin position="109"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00024347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00024945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00033987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU362114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS51977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="KAI7744562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="630" mass="71345" checksum="1D9593C33C8C59ED" modified="2024-05-29" version="1">MASNLKVDELRDHLSKRGLSTTGTKPTLVKRLESAIAKESVPVTGSDTRLAKRVREESEDGSLKIRAVDDLRGLSVKQLRQEAVLRGVDARGSKKELIERLCGDSQSNNKAVGEEIEEEKDTNENKKEKLVKATKKGSAVLDQWIPDHVKSHYHVLQQGDDIYDATLNQTNVGDNNNKFYIIQALESDDGSTYLVYNRWGRVGVKGQDKLHGPYTCVQPALDEFEQKFYAKTKNYWSDRKGFVSHPKCYTWLEMDYNEAGKENVAQEKFNSKLEIQPRETKLEARVAKFISLICNVSMMKQQMMEIGYNAEKLPLGKLSKATILKGYEVLKRVADVIGQYDRKKLEQLSGEFYTVIPHDFGFKKMREFVIDTPQKLKRKLEMVEALGEIEVATKLLEENAGAQEDPLFSCYERLSCELTPVEVDSKEYHMIAKYMKNTHAKTHTNYSVEIVQIFRTSRHGETDRFSKFSNTRNRMLLWHGSRLSNWTGILSQGLRIAPPEAPVTGYMFGKGVYFADMFSKSANYCCASQQSSSGVLLLCEVALGEMAELLEAKYDADKLPQGKLSTKGVGATAPDMSESESLEDGVVVPLGRPKKQLSSKASLLYNEYIAYNVDQIRMRYVVHVNFNFKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CN63</accession>
    <name>A0AAD5CN63_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_031027</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007701">
        <property type="protein sequence ID" value="KAI7743659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CN63"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019396">
        <property type="entry name" value="TM_Fragile-X-F-assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46859:SF3">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46859">
        <property type="entry name" value="TRANSMEMBRANE FRAGILE-X-F-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10269">
        <property type="entry name" value="Tmemb_185A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="93"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="175"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7743659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="306" mass="34899" checksum="D3DB7B3788EF6570" modified="2024-05-29" version="1" fragment="single">MRWNRIAESSEDLPAHTSLFCFTLLLVLKLHHLLSYSWRSVFIPLFVFHLLPEEGLQHLNNQYPMAFMGWGNLGKYVNQVAPNRVPWLIAFELLLLAYLSGAYVSLAIVFLPLLAFEVAIFVDNFRMCKALLQEPDDAIWLALPILLCMRLEGYPSRFANVLLLPPLGKPAAARYIPLPVVFSPIFLVQGLGVFFGTIDLVEKFGITLPTGIGTRIYFAFATRARDSFGFFLRGYRRLGRWSTEVLRRCSTKVLRRWSTEEASRDDQAPKYHDGSSGQLITILYNTFSSDPPEIVKTFPEKDLTEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CPI4</accession>
    <name>A0AAD5CPI4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein IQ-DOMAIN 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_006703</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the IQD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007564">
        <property type="protein sequence ID" value="KAI7744231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CPI4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1190">
        <property type="entry name" value="iswi atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32295">
        <property type="entry name" value="IQ-DOMAIN 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32295:SF216">
        <property type="entry name" value="PROTEIN IQ-DOMAIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="21"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7744231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="347" mass="39223" checksum="A18BD5D478F10716" modified="2024-05-29" version="1">MGKKSWFSAMKKALSPSTSKSKTKKEKRSNKSKSNPKNSCHVSLDACSSQTEAALANPSHHYHSTKETVFIASENEQIKHVDSITYTTTVAQDAVSHAAAAKVNRTRSNSRFLGRTREEVGAIKIQAAYRGYMATRAFRSLRAMRRLNMWLQGQAVKRQTTFALMRIQTMGRVQSQVRVRRIRMAEVNEALQRQLIQRRLKILEKQAFDLSPRSKEQVEASLRSKKEAAERRLKALAYAYSRQQMWRNAQSPKSGVVDPNHFDWGWSWSNRWDVIGPRETETTSNVGPMIRQQSLPSKPLVLPKSSRAQILKAELSRINTGCLAGIANKRHLVTSSSFSGAPRIAVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-04-02" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CQ26</accession>
    <name>A0AAD5CQ26_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Clp ATPase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_016256</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007018">
        <property type="protein sequence ID" value="KAI7746178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019489">
        <property type="entry name" value="Clp_ATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050130">
        <property type="entry name" value="ClpA_ClpB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11638">
        <property type="entry name" value="ATP-DEPENDENT CLP PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11638:SF18">
        <property type="entry name" value="HEAT SHOCK PROTEIN 104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10431">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01086">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Clp ATPase C-terminal" evidence="3">
        <location>
            <begin position="107"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7746178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="253" mass="28358" checksum="D551BEF87190D79F" modified="2024-05-29" version="1" fragment="single">EEILKHRTRGLGSKIAELIICPIYANLPTELQAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGLSGKTTMDKAREMVMQEVRKHFKPELLNRLDEIVVFDPLSHDQLRKVARLQMKDVAIRLADRGVALGVTEAALDVILSESYDPVYGARPIRRWLERRVVTELSKMLIREEIDENSTVYIDAGMDGNELSYRVEKNGGLVNAATGQKSDILIEVPNANGGTRSDAAQAVKRMKIEEIDDDDEVMDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CQB0</accession>
    <name>A0AAD5CQB0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Serine aminopeptidase S33 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_012835</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007497">
        <property type="protein sequence ID" value="KAI7744561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CQB0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000036">
        <property type="entry name" value="Alpha/beta-Hydrolases superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051044">
        <property type="entry name" value="MAG_DAG_Lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11614">
        <property type="entry name" value="PHOSPHOLIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine aminopeptidase S33" evidence="1">
        <location>
            <begin position="28"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7744561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="334" mass="37487" checksum="9615818BB11E3CD9" modified="2024-05-29" version="1">MEVEYQEEYIKNSRGVQLFTCRWLPLCSPKALVFLCHGYGMECGDFMKGCGTKLASYGYAVFGIDYEGHGRSMGARCYIKKFDDIVDDCSNYFKLVSGKEEYKNKKRFLYGESMGGAVALLVHKKDVHFWHGAILVAPMCKISEKVKPHPLVINILTKVEDLIPTWKIVPTKDVIEAAFKDPLKRQEIRGNKLIYQEKPRLKTALELLRTSMSLEDTLSEVTIPFFVLHGEADTVTDPEVSKALYTQASSKDKTIKLYPGMWHGLTAGETDHNIDIVFGDIISWLDKRSSDECTTTSTCSNISATASRTSQGKASLSPVQQTCGWKTPRLHSAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CSB0</accession>
    <name>A0AAD5CSB0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_024350</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SPIRAL1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006796">
        <property type="protein sequence ID" value="KAI7747308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CSB0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010005">
        <property type="term" value="C:cortical microtubule, transverse to long axis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043622">
        <property type="term" value="P:cortical microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039613">
        <property type="entry name" value="SPR1/2/3/4/5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33403:SF45">
        <property type="entry name" value="SPIRAL1-LIKE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33403">
        <property type="entry name" value="SPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0493">Microtubule</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7747308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="143" mass="15735" checksum="3476106E52FC83D9" modified="2024-05-29" version="1" fragment="single">AITQFNTIQIRDRRMRRVVTLQGIGAFGHGRTGIRRKYESRSEQWWWLEFFELLVWGEEAPKPAQKATQSETPAAKYVTTAKPDVSKQTPVGIHGNPSNGYFSADGQNTCNLIMERPSTKVHSAPGGGSSLGYLFGGGAHLGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CSS1</accession>
    <name>A0AAD5CSS1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_016257</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007018">
        <property type="protein sequence ID" value="KAI7746179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CSS1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33673:SF38">
        <property type="entry name" value="CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 7-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33673">
        <property type="entry name" value="SUPPRESSOR SRP40-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="191"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7746179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="252" mass="27588" checksum="E4E14DC3327828DE" modified="2024-05-29" version="1">MESVNATTNNGNEEILLHPDIAPDESDQIGSTKNLISLSSNTSSTSSVLSLEDLRIVDDEKHNENLSESGKTAIEVMERPETIQSHVVAKKMFTGPGNWSMASNESLFSLRMDSTNFCHLDSFSPLFDLPYDKQCVDGKILETDEGNYQGSSVLDEPAYIEDEGSRFSASTSSSSNSLVFPILLGETENDGSPYPHGSLIEPVPQSPPKPEPALDRRSKPKASGTEAEAKETTTYAPWSEWFPCFRYCACCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CUJ4</accession>
    <name>A0AAD5CUJ4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Aminotransferase class I/classII large domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_018552</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006649">
        <property type="protein sequence ID" value="KAI7747877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CUJ4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017059">
        <property type="term" value="C:serine palmitoyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004758">
        <property type="term" value="F:serine C-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046512">
        <property type="term" value="P:sphingosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050087">
        <property type="entry name" value="AON_synthase_class-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13693">
        <property type="entry name" value="CLASS II AMINOTRANSFERASE/8-AMINO-7-OXONONANOATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13693:SF3">
        <property type="entry name" value="LD36009P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="4">
        <location>
            <begin position="103"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7747877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="184" mass="20813" checksum="F8ACC332E4EA0F76" modified="2024-05-29" version="1" fragment="single">MITIPYITALTTYFSYGLLFAFGQLRDFFRNLIDWWWKDNSLQGYAPICLGLEDFYIRRLYRRIQDCFGRPISSPPDAWFDVLERVSTDNNKTLKRTTKVSRCLNLGSYNYLGFAAADEYCTPHVIQSLKKYFASTCSTRADGGTTKLHSELEEAVANFIGKPAAIVTGMGYVTNSAILPVLIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CUS7</accession>
    <name>A0AAD5CUS7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_004755</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006482">
        <property type="protein sequence ID" value="KAI7748698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CUS7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46278:SF2">
        <property type="entry name" value="ASPARTATE-SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46278">
        <property type="entry name" value="DEHYDROGENASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7748698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="122" mass="13833" checksum="9596E4640B2E3E5C" modified="2024-05-29" version="1">MTEKGTEDAGSDVTLERRKRYDFKLQTECERKGEVSEYTTAFITPPPLYHHHPHTTTNDKDVKVTAACIRVPVMRAHAESVNLQLASPLDEGPSETPYEGGVFYLDFSVPETLYSLLRFIQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CUV4</accession>
    <name>A0AAD5CUV4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">RanBP2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_007702</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">PML body</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006894">
        <property type="protein sequence ID" value="KAI7746795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CUV4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070260">
        <property type="term" value="F:5'-tyrosyl-DNA phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09080">
        <property type="entry name" value="TDP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.10.10:FF:000058">
        <property type="entry name" value="Tyrosyl-DNA phosphodiesterase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.380">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051547">
        <property type="entry name" value="TDP2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036443">
        <property type="entry name" value="Znf_RanBP2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15822">
        <property type="entry name" value="TRAF AND TNF RECEPTOR-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15822:SF4">
        <property type="entry name" value="TYROSYL-DNA PHOSPHODIESTERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00547">
        <property type="entry name" value="ZnF_RBZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90209">
        <property type="entry name" value="Ran binding protein zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01358">
        <property type="entry name" value="ZF_RANBP2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RanBP2-type" evidence="13">
        <location>
            <begin position="48"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS01358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7746795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="423" mass="47749" checksum="5C96D29F95950394" modified="2024-05-29" version="1">MSSSSSSSSWTCSRCTFINTNYSQTPTCQICLSSKPNSPSSSSNQEKWSCKACTFLNPYKLSTCEICGTKNSLVSSSLCLDDEELQVGVGNVFLPLLQRCNNNNNNNNNSKRKIGDGDDPVEVFDDSVGFSVSRGLKSANRKMVDSDEVQPKEDLRKLKILSYNVWFAEDIELRIRMRAIGDIIQLHNPDVICLQEVTPNIYAILQRYNWWKSYKCSLSFDKANARPYFCMQLSKLPVKSFSCKQFSYSAMGRELCVAEVLAQGDTPLVVATSHLESPCPGPPKWDQMYSKERVKQANEAVAFLKQNPNVIFCGDMNWDDKLDGEFPLPNGWIDAWTELHPKEIGWTYDTKSNLMLSANRKLQKRLDRFLISLHDFKVECIEMVGTEAIPEVTYMKQKKGGKELELPVLPSDHFGLLLTMAAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CVC3</accession>
    <name>A0AAD5CVC3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_033905</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010006419">
        <property type="protein sequence ID" value="KAI7748988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CVC3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022170">
        <property type="entry name" value="MUL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044231">
        <property type="entry name" value="SP1/SPL1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47568">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47568:SF2">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SP1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12483">
        <property type="entry name" value="GIDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="E3 Ubiquitin ligase MUL1-like" evidence="12">
        <location>
            <begin position="93"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF12483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7748988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="215" mass="23772" checksum="5DAA2535E0AC9290" modified="2024-05-29" version="1">MAAFAGVLCGSAAAVCYLIRLSREEAAEYYKSVAQVNQLKDLGELLKTANKVLVKVSGNVSSDTPINCKLSGLRGVIVEEKAYQNFLMQNDGGKRIQDETLMLSTNREVPWYLDDGSTRVHIVGAYNSADLLKVTCEAYEGPGQSCVCGTCDCVQSPKIFGVRRIERVLPTDTQLSVVGEVWCFYTVEKDHSLLIYFYTIYSRDGKMDRLGQLLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CVF8</accession>
    <name>A0AAD5CVF8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Methionyl-tRNA formyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_009934</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006417">
        <property type="protein sequence ID" value="KAI7749028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CVF8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004479">
        <property type="term" value="F:methionyl-tRNA formyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.170">
        <property type="entry name" value="Formyl transferase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036477">
        <property type="entry name" value="Formyl_transf_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11138">
        <property type="entry name" value="METHIONYL-TRNA FORMYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11138:SF5">
        <property type="entry name" value="METHIONYL-TRNA FORMYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53328">
        <property type="entry name" value="Formyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041953692" description="Methionyl-tRNA formyltransferase" evidence="1">
        <location>
            <begin position="26"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7749028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="111" mass="11738" checksum="E21617E33BD4B7D4" modified="2024-05-29" version="1" fragment="single">MHHLSSSSLMLRRLCCLATPSFSSSAAVFPPSSKKKKKLVFLGSPSVSASVLETLLDASSASTSLFEVAAIVTQPPSGRDRGRKVLPSPVAQHALDRGFPEDLIFTPVKAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CWR4</accession>
    <name>A0AAD5CWR4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_025911</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006279">
        <property type="protein sequence ID" value="KAI7749768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CWR4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7749768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="198" mass="22276" checksum="577759C5BB1DCF25" modified="2024-05-29" version="1" fragment="single">TSLRLPHHLPFNSLPFTNPGPYKSHKNTTVQHPNGYTCLPIIYTSGSMAIARSMCTVLLISSWAKKTVSYRQIYPTVFSDKYAFHLSKHKVDNPHAADTAWPEDVLEQLSSDNFLEQQGFCSAQLSSHPKLYKGQLENGIRYLILPTKVPQNSSSMILAELAMEADLPNDVLSIVNVTNFSWYHPTQVSSINDNDMYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CXT9</accession>
    <name>A0AAD5CXT9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_005814</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006168">
        <property type="protein sequence ID" value="KAI7750293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CXT9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7750293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="17" mass="1943" checksum="42157FCFBD5CA3C8" modified="2024-05-29" version="1">MVGSIDVMLHELKSRLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CXU9</accession>
    <name>A0AAD5CXU9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_009788</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the major facilitator superfamily. Phosphate:H(+) symporter (TC 2.A.1.9) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006171">
        <property type="protein sequence ID" value="KAI7750286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CXU9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015112">
        <property type="term" value="F:nitrate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042128">
        <property type="term" value="P:nitrate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17341">
        <property type="entry name" value="MFS_NRT2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000053">
        <property type="entry name" value="Nitrate transporter 2.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044772">
        <property type="entry name" value="NO3_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23515">
        <property type="entry name" value="HIGH-AFFINITY NITRATE TRANSPORTER 2.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0534">Nitrate assimilation</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="117"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="143"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="174"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="213"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="271"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="303"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="341"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="366"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="396"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="431"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7750286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="521" mass="56881" checksum="BEC318D83CC18A4D" modified="2024-05-29" version="1">MASSAIIPEHEEVISIPHTTNTHHHTFPLPVDSEHKATKMKIFSVAQPHMRSFHLSWLSFCTCVISTFAAAPLVPIIRDNLDLTRADISNAGIASVSGSILSRLVMGVLCDLIGPRYGCSVINLLAAPVVFSVSFIADAGGYVAVRFMIGFSLATFVSCQYWTSVMFNGKIIGVVNGVSAGWGDAGGGLTQLLMPVLFHVISQMLKTTPFTAWRIAFFVPGWFHLIVGVLVLIYGQDLPDGNFAQVRKEGGGTRDKFSKVYKNAVTNYRTWVFFFIYGYSMGVQLCLNNVISEYFYDRFSLKLHTAGVVAASFGVANFITRPFGGYLSDISARKFGMRGRLWTLWITQTLGGVFCIWLGLADSLPIAILSMMLMASGAQAACGATYGIIPFVSRRSLGILSGLTGAGGNVGGGLTQLIFFSGGRFSTAWGLTWMGVATVVFTVPVAFIHFPQWGSMFLPASKDEKYNEEYYYCSEYSEEEREMGMHIGSMKFAENSRAERGRRSVAAVAESTPDTTPNHHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CYR5</accession>
    <name>A0AAD5CYR5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">plant cystathionine gamma-synthase</fullName>
            <ecNumber evidence="7">2.5.1.160</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_009922</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>O-phospho-L-homoserine + L-cysteine = L,L-cystathionine + phosphate</text>
            <dbReference type="Rhea" id="RHEA:80891"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57590"/>
            <dbReference type="ChEBI" id="CHEBI:58161"/>
            <dbReference type="EC" id="2.5.1.160"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>O-succinyl-L-homoserine + L-cysteine = L,L-cystathionine + succinate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20397"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:57661"/>
            <dbReference type="ChEBI" id="CHEBI:58161"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-cystathionine from O-succinyl-L-homoserine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the trans-sulfuration enzymes family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.160" evidence="7"/>
    <dbReference type="EMBL" id="JAMZMK010006417">
        <property type="protein sequence ID" value="KAI7749016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CYR5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003962">
        <property type="term" value="F:cystathionine gamma-synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019346">
        <property type="term" value="P:transsulfuration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00614">
        <property type="entry name" value="CGS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000046">
        <property type="entry name" value="Cystathionine gamma-lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000033">
        <property type="entry name" value="Cystathionine gamma-synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044639">
        <property type="entry name" value="CGS1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000277">
        <property type="entry name" value="Cys/Met-Metab_PyrdxlP-dep_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054542">
        <property type="entry name" value="Cys_met_metab_PP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43379">
        <property type="entry name" value="CYSTATHIONINE GAMMA-SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43379:SF1">
        <property type="entry name" value="CYSTATHIONINE GAMMA-SYNTHASE 1, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01053">
        <property type="entry name" value="Cys_Met_Meta_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00868">
        <property type="entry name" value="CYS_MET_METAB_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00060510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00093222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00093261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00093596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7749016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="533" mass="57168" checksum="B189B5BC4B2EE5B3" modified="2024-05-29" version="1">MPILSNPSSSFQCRSHPASSGPRFSCNPRAGSLSGSTNGPFGISSLILRFPPNFVRQLSNKARRNCSNIGVAQIVAASWSDNQPLSSAAAAEAKAASSSAVSAPISAGEDVVDFNNNNNNNKLGFCNGSSVQLDDGRKISFLNGDGSVAIHAGERLGRGIVTDAITTPVVNTSAYFFKKTAELIDFKEKRHVSFEYGRYGNPTTEVLEEKISQLEGAESTLILASGMCASTVMMLALVPAGGHIITTTDCYRKTRIFIETFLPKMGITASVIDPADIAGLKSALDNHKVSLFFTESPTNPFLRCVDIELVSKLCHAKGALVCIDGTFATPLNQKALALGADIVVHSATKYLGGHNDVLGGCVSGSTEIVSQVRVVHHVLGGTINPNASYLLIRGMKTLKLRIQQQNSTAQRMAEVLEAHPKVKHVYYPGLKSHPEHELAKKQMTGFGGVVSFEVDGDITTTIKFVDSLKIPYIAPSFGGCESIVDQPAIMSYWDLSQSERGKYGIKDNLVRFSFGVEDYEDLEADVLQALEAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CZC5</accession>
    <name>A0AAD5CZC5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">WRKY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_028220</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006396">
        <property type="protein sequence ID" value="KAI7749165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CZC5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.80:FF:000003">
        <property type="entry name" value="WRKY transcription factor 57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.80">
        <property type="entry name" value="WRKY domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003657">
        <property type="entry name" value="WRKY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036576">
        <property type="entry name" value="WRKY_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044810">
        <property type="entry name" value="WRKY_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31221:SF83">
        <property type="entry name" value="WRKY TRANSCRIPTION FACTOR 75-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31221">
        <property type="entry name" value="WRKY TRANSCRIPTION FACTOR PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03106">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00774">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118290">
        <property type="entry name" value="WRKY DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50811">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="WRKY" evidence="7">
        <location>
            <begin position="120"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="73"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="73"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7749165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="204" mass="22702" checksum="4B19AE14B6C958BC" modified="2024-05-29" version="1">MDNFAPVNFPYNNSSSSTSPTAVSSHLSLNLMTDYSDHNSSYDQLEDDHDHNIGFVGKNVNCSSEELGLDHVSSATSELGNSEGNDLNSASGSGSGSGKIHVKKGDKKIRKPKCAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTYQGCNVKKQVQRLSKDEGVVVTTYEGTHSHPIEKSTDNFEHILTQMQIYSSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D036</accession>
    <name>A0AAD5D036_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">O-fucosyltransferase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_011140</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase GT106 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006280">
        <property type="protein sequence ID" value="KAI7749766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D036"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006004">
        <property type="term" value="P:fucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11299">
        <property type="entry name" value="O-FucT_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024709">
        <property type="entry name" value="FucosylTrfase_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019378">
        <property type="entry name" value="GDP-Fuc_O-FucTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31818">
        <property type="entry name" value="O-FUCOSYLTRANSFERASE 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31818:SF29">
        <property type="entry name" value="O-FUCOSYLTRANSFERASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10250">
        <property type="entry name" value="O-FucT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009360">
        <property type="entry name" value="UCP009360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-0294">Fucose metabolism</keyword>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="537"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="539"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="577"/>
            <end position="596"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00030350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7749766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="596" mass="68015" checksum="0D0DDC65658596D2" modified="2024-05-29" version="1">MAAHRRRNRHHHSHNRFRLLIPAISAISGSILIVYAIISLLAPSPVHHHHHLLRRSTTLNENVVDEDSPAVRSLFKVPLNENVVVDDLIPVHSLFKVPISGGMMSDRSLWSTRMSKYYFGCSNASNKFPKVETVTYPNRYLLIATSGGLNQQRTGITDAVVAARILNATLVVPKLDQKSFWKDSSTFSEIFNVDWFISHLSRDVKVIKELPRKGGKSLVPYRMRVPRKCNERCYQIRVLPVIMKKKAVQLGKFDYRLSNRLDTDLQKLRCRVNYHALKFTDPIIKMGQKLVNRMRKMGKHYVALHLRFEPDMLAFSGCYYGGGEKERRELGKIRKRWKTLHSNNPDKERRQGRCPLTPEEVGLMLRALGYDKDVHLYVASGEVYGGDDTLAPLRALFPNIHSKDTIATKNELEPFSAFSSRMAALDFIVCDESDVFVTNNNGNMAKILAGRRRYFGHKPTIRPNAKKLYRLFLNRENMTWGEFASGVRTHQRGFMGEPKEVRPGRGEFHENPAACICEDTEAKSKLDALPRKIGKNDADFDEVAVDQDTENEPELSDQDDEDDLIGPQFQRLVNDTSVDDDPLISESPELEEMLSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D177</accession>
    <name>A0AAD5D177_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_012779</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005711">
        <property type="protein sequence ID" value="KAI7752266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D177"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7752266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="26" mass="3094" checksum="D3C57E712233488E" modified="2024-05-29" version="1">MLEAFHTTQLWMIFEVSLKDVEALLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D2A9</accession>
    <name>A0AAD5D2A9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Shugoshin C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_028749</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the shugoshin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005918">
        <property type="protein sequence ID" value="KAI7751399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D2A9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000775">
        <property type="term" value="C:chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034090">
        <property type="term" value="P:maintenance of meiotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045144">
        <property type="term" value="P:meiotic sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044693">
        <property type="entry name" value="SGO_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011515">
        <property type="entry name" value="Shugoshin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34373:SF8">
        <property type="entry name" value="SHUGOSHIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34373">
        <property type="entry name" value="SHUGOSHIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07557">
        <property type="entry name" value="Shugoshin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Shugoshin C-terminal" evidence="4">
        <location>
            <begin position="249"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="137"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="228"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="137"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="228"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7751399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="275" mass="31762" checksum="57BEFF3570639D14" modified="2024-05-29" version="1">MGEEKMAKGSSFGKSIRKRFSDITNHDSQNKSALFFDRSSYLGKEQIDHLVKENAGLAKLITEKNKVIEMNGIELQKVRVVLQKLQSQNWNLAQSNSHMMAEINLGKKKVKELEHQLACKDALMKTMNTEYQVKRATNDQKCESKDGEKSLTNQQNASRRIRPGRSRSIGQLTTVEVSDKEMVENKRRRVRRQSARFTSQHEPDENLFEIKDLIKHENYGPTSKVDIEQEHDKCGSELEPQKSQRMSFGRPSRRAAEKVQSYKEVPVNVKMRRPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D309</accession>
    <name>A0AAD5D309_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNase H type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_001178</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005492">
        <property type="protein sequence ID" value="KAI7753263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D309"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09279">
        <property type="entry name" value="RNase_HI_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000076">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.970.10">
        <property type="entry name" value="Ribonuclease H1, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009027">
        <property type="entry name" value="Ribosomal_bL9/RNase_H1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011320">
        <property type="entry name" value="RNase_H1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037056">
        <property type="entry name" value="RNase_H1_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002156">
        <property type="entry name" value="RNaseH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46387">
        <property type="entry name" value="POLYNUCLEOTIDYL TRANSFERASE, RIBONUCLEASE H-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46387:SF2">
        <property type="entry name" value="RIBONUCLEASE HI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01693">
        <property type="entry name" value="Cauli_VI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13456">
        <property type="entry name" value="RVT_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55658">
        <property type="entry name" value="L9 N-domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50879">
        <property type="entry name" value="RNASE_H_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase H type-1" evidence="1">
        <location>
            <begin position="223"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7753263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="365" mass="39720" checksum="48E2B5E271C454D2" modified="2024-05-29" version="1">MNSLFNVCSSALFARATGNLVARCLSSGVIGLSFRRRIDHHAAIVTAGDGFLLTRFRACCYSTRKATGAGKPKTKKSDPKSKKMLGEEKNAFFVVRKGDLVGVYKNLNECQAQVGSSVCDPPVSVYKGYTMPKEAEEYMISCGLKNALYSIKAADLTEELFGNLVACPYQQPHFALGETSSDHLSKRKFPEVLGAQTGEAFGLTSISSDPARKLVKSEHHATQARTCILEFDGASKGNPGQAGAGAVLRTNDGNLICRLREGLGIATNNFAEYRALILGLRYALSKGFTTIRVLGDSKLVCMQVQGLWKVKNQNISKLYEEAKKLKDKFNCFQIDHVLRDLNSDADTQANLAVDLAVGQIQEVDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-04-02" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D496</accession>
    <name>A0AAD5D496_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GPI inositol-deacylase</fullName>
            <ecNumber evidence="1">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_010166</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in inositol deacylation of GPI-anchored proteins which plays important roles in the quality control and ER-associated degradation of GPI-anchored proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GPI inositol-deacylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="1"/>
    <dbReference type="EMBL" id="JAMZMK010005361">
        <property type="protein sequence ID" value="KAI7753648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012908">
        <property type="entry name" value="PGAP1-ab_dom-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47346:SF1">
        <property type="entry name" value="GPI INOSITOL-DEACYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47346">
        <property type="entry name" value="HYDROLASES, ACTING ON ESTER BOND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07819">
        <property type="entry name" value="PGAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0653">Protein transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="domain" description="GPI inositol-deacylase PGAP1-like alpha/beta" evidence="3">
        <location>
            <begin position="79"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="379"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="381"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU365011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7753648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="735" mass="81776" checksum="8C06D652AC81760D" modified="2024-05-29" version="1">MEGFKAKFRLVTAVVLSIGIALVAIYGLLKPISNGCTMTYMYPTYIPISATDSLSSSKYGLYLYHEGWKQIDFNEHLKQLSGVPVLFIPGNGGSYKQVRSLAAESDRAFEGGPPEPMLYQEASLTFEEGVETDETDVPIFNQYKHRLNWFAVDLEGEHSAMDGQILEEHTEYVVYAIHRILDQYKESQDARVKEGAVDTSSLPNSVILVGHSMGGFVARAAVVHPNLRKSAVETILTLSAPHQSPPLALQPSLGHYYEYINQKWRKGYEVQTSRAAPQLSRVIVISISGGGNDYQIRSKLESIDGIVPSTNGFMISSMGVKNVWLSMEHQVILWCNQLVVQVSHTLLSLVDPETGHPISGTRKRLTIFTKMLQSGMPGSLLRQSDNHQQSPHHPLQKKRNFPGSEKKDFPACPNKIHWSVEGLESDLYIKTPTVTILAMDGRRRWLDIKELGSNGRTNFVLVTNLLPCYGVRLHLWPEKGTSDSDLPLNKRVVEVTSKIVQIPSGSAPRQIEPGSQTEQPPPSAVFWLDTKDMTGFRFLTISVAPSPTVSGRPPPAASXXXXSFSTQRKGVKCFLLNRCCCLCILRRSYRWQVKWVVSRLVRSTGGMRSNGWDIFIKEDHPIVMDFMFSISLGLLPAAVSLVTTGCGIKKSGLPVEEAGDMDNGRLCKLRCFPPVALARDPTSGLHIFPNLYSRTIEVDSSPALWTSTQGSEQTNVLLLFSTFLHICYFDFSYTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D4I4</accession>
    <name>A0AAD5D4I4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_015733</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005790">
        <property type="protein sequence ID" value="KAI7751926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D4I4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7751926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="69" mass="8122" checksum="F990C0A5D2751430" modified="2024-05-29" version="1" fragment="single">IPRHVGFHEMRYHVINFFALSILDGTPDDLKSMIVETLRKECNTNLSNNNQQAVMERLKRNDSNYEIML</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D542</accession>
    <name>A0AAD5D542_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NusB/RsmB/TIM44 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_023162</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NusB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005717">
        <property type="protein sequence ID" value="KAI7752241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D542"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031564">
        <property type="term" value="P:transcription antitermination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.940.10">
        <property type="entry name" value="NusB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035926">
        <property type="entry name" value="NusB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011605">
        <property type="entry name" value="NusB_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006027">
        <property type="entry name" value="NusB_RsmB_TIM44"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11078:SF3">
        <property type="entry name" value="ANTITERMINATION NUSB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11078">
        <property type="entry name" value="N UTILIZATION SUBSTANCE PROTEIN B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01029">
        <property type="entry name" value="NusB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48013">
        <property type="entry name" value="NusB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0889">Transcription antitermination</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NusB/RsmB/TIM44" evidence="6">
        <location>
            <begin position="201"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7752241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="311" mass="34477" checksum="CE685CD18E1226D6" modified="2024-05-29" version="1">MDTSSLLIYAPLSLSSSSSSKSKITLNSSSFINYPFGKCLRWNFLPPTTTVMLSSPQASLFRTSALAVGEILEKTTLEDSSVTSLAKEQMPKVDKSGRFCSPRAARELALSIIYAACLEGSDPVRLFERRVNARREIGYEFDKETLMEYNHMSFGGPPVKTETAEEADELMRVDEKASEVEAEVLSAPPKLVYSKLILRFTRKLLVAVADKWDSHVLVIDKVAPQNWKNEPAGRILELSILHLAMSEIDVLGTRHQIVINEAVDLAKRFCDGSAPRIVNGCLRTFIKDFKGNSAAQTPDPKTQKETISSEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D6H9</accession>
    <name>A0AAD5D6H9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Non-haem dioxygenase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_021577</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010001351">
        <property type="protein sequence ID" value="KAI7755258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D6H9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.330:FF:000019">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10209:SF874">
        <property type="entry name" value="2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10209">
        <property type="entry name" value="OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7755258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="357" mass="39910" checksum="20244771C43EA2EC" modified="2024-05-29" version="1">MELPVIDLEPYLNRNTEVPTLTPQLITICSEVSTTLKETGALLVKDPRCPAQDNDRFIDMMERYFEKPDDFKRLQERPNLHYQVGVTPEGVEVPRSLVDEEMQKKLKSLPEDCQPLTPTGPDPKWRYMWRIGSRPSTTKFQELNSEPVIPEGFPEWKETMDSWGSKMVSAIEVVAEMAAIGFGLPNDAFTSLMKQGPHLLAPTGSDLKRHGKEGTVFAGYHYDLNFLTIHGRSRFPGLSIWLRNGKKVEVKVPVGCLLIQTGKQIEWLTAGECIAGMHEVVVNKRTLDAIKAASQENRSLWRVSSTLFSHIASDAVLKPLGHFAQSPLADNYPPICAGDFVEKELAVINLKGNKGAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D766</accession>
    <name>A0AAD5D766_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_024589</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010003072">
        <property type="protein sequence ID" value="KAI7754547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D766"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032039">
        <property type="term" value="C:integrator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034472">
        <property type="term" value="P:snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033060">
        <property type="entry name" value="INTS7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13322">
        <property type="entry name" value="C1ORF73 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13322:SF2">
        <property type="entry name" value="INTEGRATOR COMPLEX SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7754547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="179" mass="20006" checksum="5669A4FBC49831EC" modified="2024-05-29" version="1" fragment="single">TTLGCRCWSSGVSLPEVESCSCVDLFGEETEEEKKATEDHAAAAVNLTKSIEDADELSMAEYGLFGLVAGQDKLFANAILLRLVDMFMSVDKQTKLCVVKMFLSELKRRKNKSSNSKNRYCFSKYNVPNQLELLRRIKLCFNSGDEEVRAIGDRSDFGTTILISLSIIASRSTLRISRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D8A8</accession>
    <name>A0AAD5D8A8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Chitin-binding type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_006663</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Defense against chitin-containing fungal pathogens.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 19 family. Chitinase class I subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000962">
        <property type="protein sequence ID" value="KAI7755643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D8A8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004568">
        <property type="term" value="F:chitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016998">
        <property type="term" value="P:cell wall macromolecule catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00325">
        <property type="entry name" value="chitinase_GH19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00035">
        <property type="entry name" value="ChtBD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.20.10:FF:000001">
        <property type="entry name" value="Endochitinase (Chitinase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.530.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.20.10">
        <property type="entry name" value="Endochitinase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.10">
        <property type="entry name" value="Endochitinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001002">
        <property type="entry name" value="Chitin-bd_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018371">
        <property type="entry name" value="Chitin-binding_1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036861">
        <property type="entry name" value="Endochitinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016283">
        <property type="entry name" value="Glyco_hydro_19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000726">
        <property type="entry name" value="Glyco_hydro_19_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22595">
        <property type="entry name" value="CHITINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22595:SF193">
        <property type="entry name" value="ENDOCHITINASE EP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00187">
        <property type="entry name" value="Chitin_bind_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00182">
        <property type="entry name" value="Glyco_hydro_19"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001060">
        <property type="entry name" value="Endochitinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00270">
        <property type="entry name" value="ChtBD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="Lysozyme-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57016">
        <property type="entry name" value="Plant lectins/antimicrobial peptides"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00026">
        <property type="entry name" value="CHIT_BIND_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50941">
        <property type="entry name" value="CHIT_BIND_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00773">
        <property type="entry name" value="CHITINASE_19_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00774">
        <property type="entry name" value="CHITINASE_19_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5" id="KW-0146">Chitin degradation</keyword>
    <keyword evidence="3 9" id="KW-0147">Chitin-binding</keyword>
    <keyword evidence="6 8 9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0611">Plant defense</keyword>
    <keyword evidence="5" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042255133" description="Chitin-binding type-1 domain-containing protein" evidence="10">
        <location>
            <begin position="23"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-1" evidence="11">
        <location>
            <begin position="22"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="126"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8 9">
        <location>
            <begin position="27"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8 9">
        <location>
            <begin position="32"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="82"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="144"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="230"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001060-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001060-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7755643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="262" mass="28416" checksum="0A4E8CCB5C0FE11F" modified="2024-05-29" version="1">MKTYGMLIFSGVLLAGLLNSAAQNCGCSPDLCCSQWGYCGTGDPYCGPGCREGPCYTSDVDVGSIVTDAFFNGIVDQSDSSCEGRGFYTRATFLEALGNYAEFGTASTEGGSRREIAAFFAHVTHETGHFCYINEINGASRDYCDERYPQYPCNPNKYYYGRGPLQLSWNYNYGAAGNAIGFDGLNNPEIVGTDPLISFQTALWFWMNNVHSIIGQGFGATIRAINGMECNGGNPGTVSARVQYYTQYCSELGVTPGDNLWC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D903</accession>
    <name>A0AAD5D903_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_031984</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005258">
        <property type="protein sequence ID" value="KAI7753830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D903"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7753830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="126" mass="14192" checksum="5E8D8BEA0F5A6B9B" modified="2024-05-29" version="1">MAAANIEHDLSRLCMCTLGRDKGYKCYGYLKAAATWEVDGTQSSTGAPSSMYLLSDNRISWEVNYYIFMAWGDYGHVELLLATNAVMNPVYALSKPFWKQMDPHSGFVSDASVSYLKQEVSDYFQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D9C6</accession>
    <name>A0AAD5D9C6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_015910</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010001117">
        <property type="protein sequence ID" value="KAI7755445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D9C6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766:SF61">
        <property type="entry name" value="NB-ARC DOMAIN DISEASE RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766">
        <property type="entry name" value="PLANT BROAD-SPECTRUM MILDEW RESISTANCE PROTEIN RPW8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0611">Plant defense</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7755445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="648" mass="73211" checksum="978EC9737AE5CB2A" modified="2024-05-29" version="1">MDDVEVIGSELTGTTTVVAFPSLKILKFENMCAWKAWSTNTMVLDVVFPCLQELHIKDCPNLIDISLKALPSVRVLQIYTCSVNALRSIVQAASLVNELGIASIVGLTDEAWRGVIMYLKEVENLSIWHCDEIRYLWELEDMNKVLDVRRCKNLVSLGEKEEDNFESTLLLSLTYLEIYYCESMKHCYCPNSIEYFGVEGCTSLTHVSFPRAIGGGMEKLKTLVISDCKKLTEEINNVSMPMLDFLYISDQWTGWKSITQFSNLNHLTVLYIANCPSIETFQLSNLTSLTYLRITNCTSFCLSFDGGFWPPNLVFLDIGGLKTPISEWGPPNFPSSLVDLTLSRDLDVSDFRQLSHLFPSSLTSLKIEIFLKLESLSTGLQHLSSLQHLKIEKCLMMKHLPVSLLPSLSSLKIALCPYLTLRCNGRGSHYWSRISHIPCIQIDHSKDMEELMENFRNMSKRQEWVDPHSYKCDLTQSTDTKLAGLSSNVFVSSAKPPRKNSANIFSNRKMNGKAVSFIGRSGSREAISQLSNSTHLTRLDISDCPHILSLFDLHLSNLTFLSLCGLWPPKLCSLEIGKLKKAISEWGDLSYGMNPIKITMTESANNNQSIDTSLLWELLLTACEVKKVLDLIMFLVMQVHWSLKRFGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D9S0</accession>
    <name>A0AAD5D9S0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Expansin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_009868</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Causes loosening and extension of plant cell walls by disrupting non-covalent bonding between cellulose microfibrils and matrix glucans. No enzymatic activity has been found.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Secreted</location>
            <location evidence="7">Cell wall</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Membrane</location>
            <topology evidence="7">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the expansin family. Expansin A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000341">
        <property type="protein sequence ID" value="KAI7756558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D9S0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009664">
        <property type="term" value="P:plant-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22274">
        <property type="entry name" value="DPBB_EXPA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.10:FF:000001">
        <property type="entry name" value="Expansin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.760:FF:000001">
        <property type="entry name" value="Expansin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.760">
        <property type="entry name" value="Expansin, cellulose-binding-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="entry name" value="RlpA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007118">
        <property type="entry name" value="Expan_Lol_pI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002963">
        <property type="entry name" value="Expansin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007112">
        <property type="entry name" value="Expansin/allergen_DPBB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007117">
        <property type="entry name" value="Expansin_CBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036749">
        <property type="entry name" value="Expansin_CBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009009">
        <property type="entry name" value="RlpA-like_DPBB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31867">
        <property type="entry name" value="EXPANSIN-A15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03330">
        <property type="entry name" value="DPBB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01357">
        <property type="entry name" value="Expansin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01226">
        <property type="entry name" value="EXPANSIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01225">
        <property type="entry name" value="EXPANSNFAMLY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00837">
        <property type="entry name" value="DPBB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="Barwin-like endoglucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49590">
        <property type="entry name" value="PHL pollen allergen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50843">
        <property type="entry name" value="EXPANSIN_CBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50842">
        <property type="entry name" value="EXPANSIN_EG45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0134">Cell wall</keyword>
    <keyword evidence="6 7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041769032" description="Expansin" evidence="7">
        <location>
            <begin position="22"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="Expansin-like EG45" evidence="8">
        <location>
            <begin position="50"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="Expansin-like CBD" evidence="9">
        <location>
            <begin position="174"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7756558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="258" mass="28141" checksum="56EAA6E44A35A881" modified="2024-05-29" version="1">MEVMTLAYFLLLSTILTSINARIPGVYIGGQWESAHATFYGGSDASGTMGGACGYGNLYSQGYGVNTAALSTALFNNGLSCGACFEIKCANDPQWCHPGSPSIFITATNFCPPNFAQASDNGGWCNPPRTHFDLAMPMFLKIAQYRAGIVPVSYRRVPCRKQGGVRFTINGFRYFNLVLITNVAGAGDITQAWVKGSRTGWMSLSRNWGQNWQSNAVLVGQSLSFRVRGSDRRTSTSYNIVPANWKFGQTFVGKNFRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DB77</accession>
    <name>A0AAD5DB77_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_018679</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000036">
        <property type="protein sequence ID" value="KAI7757958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DB77"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007930">
        <property type="entry name" value="DUF724"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05266">
        <property type="entry name" value="DUF724"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0341">Growth regulation</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="89"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="214"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="103"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7757958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="310" mass="34835" checksum="A4CD48B1E09243C3" modified="2024-05-29" version="1" fragment="single">GSKGNTKAKVSVTEKRGSDANNQDSPVVEHEMIIAETCEAADGKSHPKKKRGRSSTLLAKGPINLQEGEHEITVDLDEQPLSQLAGLKRSSCHRPEQRDEPPNQATVTSAGTTDYEQNWPFIKRSPIWATIASLEVYQTPPQRPHFSPLKEIKEDHREGLAIAHMVTFGNQVQMFSEIKLTDPVNVINNSLEILGDLETHGFNVGAIRGRLNKLLSLKSKVSQHEIELKKVEAELEKRNGEKRAEEEEIKEMEKKMQELQEKMVRSATMMKVKEEETTRLQSDMHNLSEQITDTELAFKKLAATPLQGTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DC21</accession>
    <name>A0AAD5DC21_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_017552</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000310">
        <property type="protein sequence ID" value="KAI7756675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DC21"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030247">
        <property type="term" value="F:polysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013695">
        <property type="entry name" value="WAK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025287">
        <property type="entry name" value="WAK_GUB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33491">
        <property type="entry name" value="OSJNBA0016N04.9 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13947">
        <property type="entry name" value="GUB_WAK_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08488">
        <property type="entry name" value="WAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Wall-associated receptor kinase" evidence="7">
        <location>
            <begin position="86"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="Wall-associated receptor kinase galacturonan-binding" evidence="8">
        <location>
            <begin position="275"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7756675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="600" mass="66074" checksum="5DF6899303345285" modified="2024-05-29" version="1">MEVLDISLKEQTVTVNISVISRCRSSVLNSNHIQSVDLGDSPFVFSASHNILNVEGCGYAAILNYGEIISGCSTTCNSDISNETDLCIGLGCCQTKIPGNLKSYTVDVTGLKLLGEDGACGSAFLGDESWLPSYNTNGYSLQTRNKSFFPVSLMWLLTENEYDEMPNCRRRKSRNPFVDLGNGSYIDLKKCSCGIGHFGNPYLPYLCEDSGQCTSCLDSGGTCHFEETYPDDLDDTWSFYCQPKIYYPASSSKSTLGIILAKSSAADPDYAKKGCNDTCGGVRIPYPFGIGANCSVNKWYVIDCNSSTPYLSAFNNMEVVKVSLEEQTVTVNVSVISRCQNSDDHIPSMDLGESPFVYSGSHNVLTVEGCGYAAILDYRPEVIAGCSTTCTNDGTSNGSDLCFGLGCCRITIPYNLKSYSLDLTGLKRLGEDGACGSAFLADERWLPYSFRFSDGNNLPEIPKGKTRNTSFSQITLMWFLTNDDFLEIPNCWEEKTGESSLYLGNRSYINQRMCSCGVGRVGNPYLHYQCEESEDCRRCRDSGRICLYEQIYLGNSDDSWWLNCSEPAVYNPISSSSKSPITGVIIGKLYIISFNRTFRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DCB6</accession>
    <name>A0AAD5DCB6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_008767</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000578">
        <property type="protein sequence ID" value="KAI7756111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DCB6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7756111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="111" mass="12595" checksum="F3FD786C2D16892D" modified="2024-05-29" version="1">MKDEYDPMVKVRHTNTNTVGLSPHYAFRSNSWDHARSQQMLMDRELAAPGLVNSSLCHKQAHGQQKSSELIAIDANFMQSQSHREGSTSFAIKKLRRETAVLRENRNPCGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DCW5</accession>
    <name>A0AAD5DCW5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Hydrophobic seed protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_023773</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000147">
        <property type="protein sequence ID" value="KAI7757252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DCW5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01958">
        <property type="entry name" value="HPS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.110.10">
        <property type="entry name" value="Plant lipid-transfer and hydrophobic proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036312">
        <property type="entry name" value="Bifun_inhib/LTP/seed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027923">
        <property type="entry name" value="Hydrophob_seed_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051636">
        <property type="entry name" value="Plant_LTP/defense-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31731">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14547">
        <property type="entry name" value="Hydrophob_seed"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47699">
        <property type="entry name" value="Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042221939" description="Hydrophobic seed protein domain-containing protein" evidence="2">
        <location>
            <begin position="27"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="Hydrophobic seed protein" evidence="3">
        <location>
            <begin position="203"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="165"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="173"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7757252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="288" mass="29369" checksum="5CC7A11E485C8E30" modified="2024-05-29" version="1">MGSKISAMFYIFLICMSSTSLPLVFGCKHCPPCSPPHHQPHPPSTKPPHHGGHGGKGSPPKTRPPKTRPPIVLPPIVCPPPVTRPPKGPPIVLPPIVMPPPVTRPPKTPPIVLPPIVMPPPVTRPPKTPPIVLPPIVMPPPVIVPPPVLPPPILPPPIVMPPPPYSSPPYTPPGGGKGGGGKGGGGKGGGGKGGGGGGVMPKCPINALKLGACVDVLGGLVHIGLGDPVENVCCPLLKGLLELEAAVCLCTTIRLKLLNLNIFVPLALQALVTCGMTPPPGFVCPPLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DD48</accession>
    <name>A0AAD5DD48_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_031437</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000138">
        <property type="protein sequence ID" value="KAI7757309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46008">
        <property type="entry name" value="LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-LIKE 1.4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46008:SF25">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="241"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="308"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="405"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="405"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="337"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7757309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="434" mass="49156" checksum="90E8AEA2E469AF7D" modified="2024-05-29" version="1" fragment="single">MPVCAESFSCPGLGQLKYPFYNGTDRKCGLIKINCTSKEVQFGGWSYEFFGKFDSGQKYDNIIIHNKTFEQLLKTNSCEALMDNFTSPRPLLYSISIFPFITLFKCTKNRTYAQETDVYFERQNHFNSYNKCKYHNFYYNYFNGTVPSGLPHTCQVVKLPAQLSSSPGLNETDIVSLLSSYTSVTFNLSHSCSECRKKGRLCDTENERDVYCFDIEKAVTPGREPTPSPNQVPAGKQVRELINILVIVGSALIIILFVVVFIIWRHCKSNPFSYASAKNKSRNLEDISFSSGVSVFSYEELEDATQNFDPSHELGDGGFGAVYYGKLHDGRKVAVKKLHEHTYNRIHQFRNEVXXXYEHDHISNVGRLDTYGSTRDLQTANVLPLSETNDVVVLLKDFQSSPFSVSNELQSNNSASTTLRSNEARLSMKNNIDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DDI2</accession>
    <name>A0AAD5DDI2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_023608</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in the transport of sterols.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000768">
        <property type="protein sequence ID" value="KAI7755840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DDI2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13294">
        <property type="entry name" value="PH_ORP_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.120:FF:000012">
        <property type="entry name" value="Oxysterol-binding protein-related protein 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037239">
        <property type="entry name" value="OSBP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000648">
        <property type="entry name" value="Oxysterol-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972:SF96">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN-RELATED PROTEIN 1A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01237">
        <property type="entry name" value="Oxysterol_BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15413">
        <property type="entry name" value="PH_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144000">
        <property type="entry name" value="Oxysterol-binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="4" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PH" evidence="6">
        <location>
            <begin position="5"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="69"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="241"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="77"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="259"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="287"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="307"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="502"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7755840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="502" mass="56900" checksum="62737A474622A206" modified="2024-05-29" version="1" fragment="single">NDIVGDGISGVLNKWVNYGKGWRPRWFVLQDGVLSYYKIHGPHKIVVSKETEKGARVIGDESFRRLSRHRGSGVNGIGNNNNNSSSSHRPYRKPVGEVHLKVSSVRESRSDDRRFSIFTGTKRLHLRADTRDDRIAWMEALQAVKGMFPRMSNSELMAPIGGVTLSTEKLRQRLLEEGVSEAAIQDSETIMMTEFSAMQDQLVLLKQKHLLLMDTLRMLETEKVDLENTVVDESQRLSKEVGATSRFRQEKYSDASPSESEDDNDRGDAAEEESDDEDNAFFDTRDFLSSGSFKSSGSDMRSSSFTSDDEDSQFNEPEIDASIRSVGTSFPRVKRRKKLPDPVEKEKGGNSLMRILNVAAFAVSGYSSTEERSCKPFNPLLGETYEADYPDKGLRFFSEKVSHHPMIVACHCEGKGWKFWGDSNLKSKFWGRSIQLDPVGVLTLEFDDGEIFQWSKVTTSIYNLILGKLYCDHYGTMRIQGNQNYSCKLKFKEQSIIERNPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DDP1</accession>
    <name>A0AAD5DDP1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_002763</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000115">
        <property type="protein sequence ID" value="KAI7757470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DDP1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044274">
        <property type="entry name" value="RFI2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46798">
        <property type="entry name" value="OS09G0511500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46798:SF3">
        <property type="entry name" value="RING FINGER FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="212"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="80"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="226"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="254"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="311"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7757470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="320" mass="34286" checksum="860784C2370BD99B" modified="2024-05-29" version="1" fragment="single">SMWCPISGFTRLTASFDEGDFPTTAYHDFLGQHAIFTEHAATVSSAGHLCPYFAYVQPVHPSSSSSTSNANVADGPTPTPTSTQYNNNTSNSSSNNQWNSIQSASSEMPNSYALPTMDVRYHTSLFPPSNNRVGVVGADQPSMPSVMPRRPVRNNADIPRSASYAHPFIVGQNSAARVPSSVGSSMIHPHPGSAARARERTQALQAYFQQPSSLPGVRTPMGPGSRRSNSQAQMGQVGSSSDQIHGGGVYYLPTSGSSTTRSGFQEAESSMPNPFHHTWERDQHLGPGWGFQHSGGASGGAVFRVRHGSDRTPSQGRYRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DE30</accession>
    <name>A0AAD5DE30_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_008766</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000578">
        <property type="protein sequence ID" value="KAI7756110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DE30"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7756110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="96" mass="10985" checksum="071EEDBDD336C289" modified="2024-05-29" version="1">MKDEYDPMVKVRHTNTNTVGLSPHYAFRSNSWDHARSQQMLMDRELAPGLVNSSLCHKQAHGQQKSSELIAIDANFMQSQSHREVRLGQHLSLLRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DF16</accession>
    <name>A0AAD5DF16_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_027662</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000017">
        <property type="protein sequence ID" value="KAI7758197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DF16"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15725:SF18">
        <property type="entry name" value="ZINC FINGER, CCCH-TYPE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15725">
        <property type="entry name" value="ZN-FINGER, C-X8-C-X5-C-X3-H TYPE-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="75"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="179"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="250"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="139"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="202"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="259"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7758197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="368" mass="42438" checksum="8DAE55C8962D33C9" modified="2024-05-29" version="1">MVTNQQPQYSQLEELLKTNTDCLYFLASPLTCIKISAFSTLLYNLTKLRLHYHLLTGKWRVACIFFQKGPEKSVQEQKFPPQENVQKPTEFPPQEKQVPRAQQRNGGAVMEKKVAPPPEEPPRYKPAGVVLPPVYDSNHVSDNEEQFGRSRGQDFDLGRSVDYDVNHYNKEGYAWEDHRESNGFGRTQYPRDESPDQLNSSDLRHRLSKQRRDNNVGGLRREAHYNQRHDCCLDSGPLSNRLRGRIKIPGRAMSPGYEHGSRVKRDIDMGTQQRSRYSPGRSPHVSSNNSRVKADYEKSTNDQALLNLEVSDVANKVGSIDEDAMLDQELEAYDHREDFNLEEGEEYMEEGYETGKKQSEMYSWIQSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G3X3</accession>
    <name>A0AAD5G3X3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">WEB family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_014892</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WEB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011406">
        <property type="protein sequence ID" value="KAI7727257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G3X3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009904">
        <property type="term" value="P:chloroplast accumulation movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009903">
        <property type="term" value="P:chloroplast avoidance movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32054">
        <property type="entry name" value="HEAVY CHAIN, PUTATIVE, EXPRESSED-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32054:SF31">
        <property type="entry name" value="PROTEIN WEAK CHLOROPLAST MOVEMENT UNDER BLUE LIGHT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7727257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="167" mass="19115" checksum="F63557F70E447B03" modified="2024-05-29" version="1" fragment="single">MSRSEIDTSAPFKSVREAVALFGERVLAGEVYGNKRKEIKNEESENVPAPVIIELEETKQRLEEAREDRMLMATCLSSLQRELERTKCELLQLKESSKVDEEDVKFIQDVEVNTSHSGVEFQKKRYVTFAKPPSVEHVIIPSCDATVLERQPSMKKKKKKKPLIPLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G578</accession>
    <name>A0AAD5G578_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Response regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_010192</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011301">
        <property type="protein sequence ID" value="KAI7727903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G578"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009736">
        <property type="term" value="P:cytokinin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045279">
        <property type="entry name" value="ARR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43874:SF214">
        <property type="entry name" value="RESPONSE REGULATORY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43874">
        <property type="entry name" value="TWO-COMPONENT RESPONSE REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="1" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="Response regulatory" evidence="5">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7727903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="78" mass="8871" checksum="5E4045CD4EDA1E3A" modified="2024-05-29" version="1">MPGMTGYDLLKKIKASCDIKEIPVIIVSSENVPTRIEKCLEEGAQEFILKPLKQSDVKKLRCQMQFTQPMKGRLCMGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G5H4</accession>
    <name>A0AAD5G5H4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GED domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_011072</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010968">
        <property type="protein sequence ID" value="KAI7729595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G5H4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1240:FF:000018">
        <property type="entry name" value="Dynamin-related protein 3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1240">
        <property type="entry name" value="Dynamin, middle domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022812">
        <property type="entry name" value="Dynamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000375">
        <property type="entry name" value="Dynamin_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003130">
        <property type="entry name" value="GED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020850">
        <property type="entry name" value="GED_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566">
        <property type="entry name" value="DYNAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566:SF21">
        <property type="entry name" value="DYNAMIN RELATED PROTEIN 1, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01031">
        <property type="entry name" value="Dynamin_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02212">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00302">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51388">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0505">Motor protein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GED" evidence="3">
        <location>
            <begin position="202"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="77"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="299"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="82"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="111"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="316"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7729595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="348" mass="39171" checksum="2CB3152194600D83" modified="2024-05-29" version="1" fragment="single">MSHRCMVNELQRFPVLRKRMDDVTGNFLRDGLQPSETMIGHIVEMEMDYINTSHPNFIGGSKAVELAMQQVKSSRIASAVSRQKDAAEPDKAPQSERSIKSRTFLGRQGNGIVTDQHNQPGSRPAGEAEKQPSGSTSWGISSIFGGSDSRMSNKDNSANKPFSEPVSNVDHLDYSSSMIHLREPPTVLRPSDANSDQEAIEIHVTKLLLRSYYDIVRKNIEDYVPKAIMHFLVNHTKRELHNVFIKKLYRDDLFEQMLQEPDDISIKRKRTRETLRVLQQAFRTLDELPLEAETVERGYSLTNNDSTGLPKIHGLPTSSMFPSGSGSMDPYNTASPKNQRSSRKSSHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G6D2</accession>
    <name>A0AAD5G6D2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">M8C21_033298</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="24">AA19_3_7</strain>
            <tissue evidence="24">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RLP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010010842">
        <property type="protein sequence ID" value="KAI7730092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G6D2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033612">
        <property type="term" value="F:receptor serine/threonine kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000317">
        <property type="entry name" value="Inactive leucine-rich repeat receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000388">
        <property type="entry name" value="Leucine-rich repeat receptor-like tyrosine-protein kinase PXC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000111">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.310:FF:000001">
        <property type="entry name" value="Serine/threonine-protein kinase BRI1-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000150">
        <property type="entry name" value="serine/threonine-protein kinase BRI1-like 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045381">
        <property type="entry name" value="BRI1_island_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050647">
        <property type="entry name" value="Plant_LRR-RLKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48056">
        <property type="entry name" value="LRR RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48056:SF18">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20141">
        <property type="entry name" value="Island"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="15 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-0675">Receptor</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9 22" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="22">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042289792" description="non-specific serine/threonine protein kinase" evidence="22">
        <location>
            <begin position="23"/>
            <end position="1037"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="663"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="758"/>
            <end position="1015"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="787"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="KAI7730092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="1037" mass="113111" checksum="433BDD746EFA1CDD" modified="2024-05-29" version="1">MKHLTNFFVFLTLLHCIISTYGDDTQQQFTILKSSLKNTALLPDWLPSNSVCNYTGVHCRNSRVSAIDLSNIDLSVEFGLISSTLLTIPTLESFVGKNCNVTGTVLVRSQCSKVLKVLDLSNNRVSGSVADVSVLATCTSLKILNFSRNSFEFNGAEKPLGLLVSTQVVDFSYNRISGPDVVQWMLSDGCGELLEFNVSGNNISGTVPDSLKGCSLLQVFDISHNNFTGELPMDLFMGLSSLKSLGLGFNNFKGTLPDSLSEMTNLERFDVSSNQISGEIPPGICGLGFGSGLKVLYLQNNRLSGSIPESIGNCSELVSLDLSFNSLIGNIPKSFRNLSKLQDLIIWMNLLTGEIPEELMYLSNLENLILDFNYLTGSIPASLSNCTNLNWISLSNNNLFGEIPAALGGLSNLAILKLGNNSFSGKIPPELGDCKSLVWLDLNTNQLSGTIPPALFKQSGFIAAAYLTGKPYLYIKNDGSKQCHGAGNLLEFGGIRQEDLDRISSRHPCNFTRVYLGITQPNFNHNGSMIFFDLSYNKLEGGIPKELGTMYYLNILNLGHNDLTGPIPEELSGLKNVAILDLSHNRLNGSIPNSLTSLGLGDADLSFNNLSGSIPVSAPFDTFPADRFSNNSGLCGYPLPKCGSDKVDKPNGHRKSNKREASLAGSVAMGLLFSLFCIFGVIIILVEMRKRRRKKAAAALEAYTDGGGHSYSGGDRANASTAWKLTTTREALSINLAAFEKPLRKLTFADLLEATNGFDNNSLIGSGGFGDVYRAQLKDNTVVAIKKLIHVSGQGDREFTAEMETIGKIKHRNLVPLLGYCKVGDERLLVYEYMKFGSLEDVLHDRKKTGIKLNWAKRRKIAIGSARGLAFLHHNCIPHIIHRDMKSSNVLLDENLEARVSDFGMARHMSAMDTHLSVSTLAGTPGYVPPEYYQSFRCSTKGDVYSYGVVLLELLTGKQPTDSPDFGDNNLVGWVKLHAKTCITDVFDPELLNEDPTLKVELLQHLKVACLSAFRVCGLELGNVLEVSRFAPMGYGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G6S0</accession>
    <name>A0AAD5G6S0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Dynamin-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_011073</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010968">
        <property type="protein sequence ID" value="KAI7729596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G6S0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08771">
        <property type="entry name" value="DLP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022812">
        <property type="entry name" value="Dynamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001401">
        <property type="entry name" value="Dynamin_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019762">
        <property type="entry name" value="Dynamin_GTPase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045063">
        <property type="entry name" value="Dynamin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030381">
        <property type="entry name" value="G_DYNAMIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566">
        <property type="entry name" value="DYNAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566:SF21">
        <property type="entry name" value="DYNAMIN RELATED PROTEIN 1, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00350">
        <property type="entry name" value="Dynamin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00195">
        <property type="entry name" value="DYNAMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00053">
        <property type="entry name" value="DYNc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00410">
        <property type="entry name" value="G_DYNAMIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51718">
        <property type="entry name" value="G_DYNAMIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynamin-type G" evidence="2">
        <location>
            <begin position="1"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7729596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="197" mass="21807" checksum="3C0807B48A649642" modified="2024-05-29" version="1" fragment="single">ALVGRDFLPRGSDICTRRPLVLQLVQIKRKPDGTEEEYGEFLHKVGKRFYDFNDIRREIQAETDKEAGGNKGVSDKQIRLKIFSPNVLDITLVDLPGITKVPVGDQPSDIEARIRTMIMSYIKLPSCLILAVTPANSDLANSDALQIAGNADPDGYRTIGVITKLDIMDRGTDARNFLLGKVIPLRLGYVGVVNRSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G8B2</accession>
    <name>A0AAD5G8B2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_012239</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010287">
        <property type="protein sequence ID" value="KAI7732204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G8B2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000223">
        <property type="entry name" value="probable receptor-like protein kinase At1g80640"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000342">
        <property type="entry name" value="Protein kinase superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47989">
        <property type="entry name" value="OS01G0750732 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47989:SF27">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042080407" description="Protein kinase domain-containing protein" evidence="9">
        <location>
            <begin position="19"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="66"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="149"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7732204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="444" mass="48346" checksum="BCB2979C7B67A54F" modified="2024-05-29" version="1">MNLFLGLLLFLNLGFVIASIDMVSSISAPESSPMAAAPIALVPSSMADFTSGPNVGNHRMKANRKLLIGLVTSLLVIMIIIMSIIVLMKCHRRKLQKLKNPGGKRSDSLRGLALSSFVGKSNGSLKTVNEKASVAVMDYHLLESATGNFGEGEVLGEGGFGCVYRARIDENLCVAVKRLDGGSQDAIKEFQTEIDLLSKIQHPNIITLLGYCVHDDTRLLVYELMHNGSLETQLHGPSNGSNLTWHCRMKIALDTARGLEYLHENCKPSVIHRDLKSSNILLDSSFNAKLSDFGLAVMDGAQNKSNVKLSGTLGYVAPEYLLDGKLTDKSDVYAFGVVLLELLLGRRPVEKLAASQCQSIVTWAMPQLTDRSKLPNIVDPVIRYTMDLKHLYQVAAVAVLCVQPEPSYRPLITDVLHSLIPLVPVELGGTLKVTQTRSSVTVDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G9P7</accession>
    <name>A0AAD5G9P7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_030278</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SWEET sugar transporter family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009715">
        <property type="protein sequence ID" value="KAI7734470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G9P7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051119">
        <property type="term" value="F:sugar transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.290:FF:000002">
        <property type="entry name" value="Bidirectional sugar transporter SWEET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047664">
        <property type="entry name" value="SWEET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004316">
        <property type="entry name" value="SWEET_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10791:SF159">
        <property type="entry name" value="BIDIRECTIONAL SUGAR TRANSPORTER SWEET5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10791">
        <property type="entry name" value="RAG1-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03083">
        <property type="entry name" value="MtN3_slv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="45"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="76"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="107"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="141"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7734470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="179" mass="20110" checksum="CAC45CDA6043AD38" modified="2024-05-29" version="1">MFYGLPIVHPNSLLLITINGAGLLIEIIFITIFFLYSTWDGRKKILLILFIEAVVVALIAVVTLMCFDTYKSRSMVVGLIAIVCNTLMYAAPLTVMKMVIKTRSVKYMPFSLSLACFVNSIVWCVYALMPLDPYILVPNGVGSLSATLQLILYATYYGTTKWDDHYEKREVRMSPTSNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GBZ1</accession>
    <name>A0AAD5GBZ1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Core-2/I-branching beta-16-N-acetylglucosaminyltransferase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_032331</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009168">
        <property type="protein sequence ID" value="KAI7736900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GBZ1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044174">
        <property type="entry name" value="BC10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003406">
        <property type="entry name" value="Glyco_trans_14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31042">
        <property type="entry name" value="CORE-2/I-BRANCHING BETA-1,6-N-ACETYLGLUCOSAMINYLTRANSFERASE FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31042:SF138">
        <property type="entry name" value="GLYCOSYL TRANSFERASE, FAMILY 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02485">
        <property type="entry name" value="Branch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7736900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="306" mass="35577" checksum="1C6EFC41A01D0858" modified="2024-05-29" version="1">MMSGVRQRPNFKGTNWPRNTAACYIFLSRGCSGHENTPAVPSRELTDEENAARVVITEILKSSPPTPKNPKVAFMFLTPGPLPFEMLWDKFFQLLRGGKNGRIGSASERVKVMRVDLVFMCMPQENSRLIYDDPGPHGAGGRYSEHMLPEIEFKDFRKGSQWFTMTRKHALIIMADSLYYRKFRLYCRPGFEGRNCYADEHYFQTLFYMIDPAGIANWSVTYVDWSERKWHPRSYKPQDITPKLLRNLTSFDTSQHFTSDEKKTVLTTHCMWNGMKRPCFLFARKFYPETLDNLLQLFSNSTSIPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GDD8</accession>
    <name>A0AAD5GDD8_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_013984</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008872">
        <property type="protein sequence ID" value="KAI7738100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GDD8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016125">
        <property type="term" value="P:sterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24286:SF228">
        <property type="entry name" value="C-22 STEROL DESATURASE ERG5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24286">
        <property type="entry name" value="CYTOCHROME P450 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7738100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="241" mass="27405" checksum="20F61E8B0CD8FBA6" modified="2024-05-29" version="1" fragment="single">MAKSSGLGFSANYIIGRFIVFIRSTDLSHKVFANVRPDAFHLVGHPFGKKLFGEHNLIYMMGQQHKDLRRRIAPNFTPKALSTYTELQQHIILKHLKSWLDLPNSSTKPFPLRLLCRDMNLETSQNVFVGPYLSGESRKRFEVDYNYFNVGLMKLPIDLPGFAFRNARLAVSRLVKTLAVCAEDSKKKMKSGEDPTCLVDFWMQDTIREITLAEDVAVNPPPHTSSIEIGGHLFDFLFASQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GEB7</accession>
    <name>A0AAD5GEB7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_032444</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009067">
        <property type="protein sequence ID" value="KAI7737291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GEB7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046474">
        <property type="term" value="P:glycerophospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000137">
        <property type="entry name" value="Hydrolase family protein / HAD-superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050324">
        <property type="entry name" value="CDP-alcohol_PTase-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006357">
        <property type="entry name" value="HAD-SF_hydro_IIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006353">
        <property type="entry name" value="HAD-SF_hydro_IIA_CECR5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01456">
        <property type="entry name" value="CECR5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01460">
        <property type="entry name" value="HAD-SF-IIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14269">
        <property type="entry name" value="CDP-DIACYLGLYCEROL--GLYCEROL-3-PHOSPHATE 3-PHOSPHATIDYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14269:SF49">
        <property type="entry name" value="HYDROLASE FAMILY PROTEIN _ HAD-SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13344">
        <property type="entry name" value="Hydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13242">
        <property type="entry name" value="Hydrolase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7737291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="381" mass="42698" checksum="8CE326B69C3E13BA" modified="2024-05-29" version="1">MKFRTVFRVLHHRNRSPSIQLHTFRLIAHLHSSPNRHSSFGIAFDIDGVILRGRTPIGDSPKALKRLYDDDSGSLKIPYLFLTNGGGIPETRRAAELSRLLGVNIDPIQVVQGHSPFKNLLARFENELVVATGKGEPAVVMSEYGFKKAISLEDYASYFNNIDPLAPFKTWTTKQPCTDKSKYSESVLSFDVTSERVKAAFVVSDPVDWSRDIQVLCDILRSGGLPGEKSEHQPHMYFAADDLEYQAVFPSERLGMGAFRIALENIFNSIHHKPLKYTSFGKPNPFVFKNAESILMQLQSLTSDADLNGGYENMKSHHFETLYMIGDNPSVDIKGARQAGHPWFSILTRTGVFRGKVNHTEYPADKLTVQSNACCYVIIAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GEY4</accession>
    <name>A0AAD5GEY4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">S-adenosyl-L-methionine-dependent methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_032155</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008927">
        <property type="protein sequence ID" value="KAI7737891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GEY4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000236">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051419">
        <property type="entry name" value="Lys/N-term_MeTrsfase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12176:SF76">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE-DEPENDENT METHYLTRANSFERASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12176">
        <property type="entry name" value="SAM-DEPENDENT METHYLTRANSFERASE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="13"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7737891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="316" mass="33891" checksum="A851BF1C87E7F14E" modified="2024-05-29" version="1" fragment="single">RASHPHISSSSNNKKKSKSKHTTQHEGQGGWLKVVSKCCAAVEEGGNGEEVDNNYQVLTAINTSYNDILILDSPHSRMLLLDPTHNIHSIFMKGQPWTGSYWDEFASLPPIVPQGPIAIFGLGGGTAAHLMLTLWPSLQLHGWEIDEILIDKAREHLGLSHLEKPSQGAAADGGGVLHVHIGDALSASTTISGGYAGIVIDLFAGGEVLPQLQQVQTWLQLNDKLMPNGRLMINCGGTTSLSGSTDSSTDAAWDQNSTLKTLCEAFPGQVNWKKMPKSEGENYLALTGPLPDLTLWAAALPDRLGSGVVQWACCFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GFF1</accession>
    <name>A0AAD5GFF1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_010300</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the peroxisomal membrane protein PXMP2/4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008879">
        <property type="protein sequence ID" value="KAI7738088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GFF1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007248">
        <property type="entry name" value="Mpv17_PMP22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266:SF18">
        <property type="entry name" value="OS12G0508100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 2, PXMP2 MPV17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04117">
        <property type="entry name" value="Mpv17_PMP22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="132"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="167"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="205"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="234"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7738088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="261" mass="29264" checksum="C2FC3F8BF34B8BC2" modified="2024-05-29" version="1">MCACFLKNNGNKAHLYINALWKTRSVSNSIGAPITHRPHFHQPKASYSRFPQRKVKEMDFPVPFAGSNTSTKGFSSVSNNRLIGWYLGLVKTRPILTKSITSAFISTAADLSSQTIAGSSSESYDPVRTVRMAGYGMIVLGPSLHFWFNFMSRLFPKRDLISTFKKMFMGQAIFGPIMTVVFFSVNAGLQGESGSEIVGRLKRDLFPTLINGVMYWPVCDFITFRFIPVHLQPLVSNSFAYIWTVYMTYMASLEKAGTDTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GG08</accession>
    <name>A0AAD5GG08_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_030790</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008750">
        <property type="protein sequence ID" value="KAI7738688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GG08"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14703">
        <property type="entry name" value="bZIP_plant_RF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000009">
        <property type="entry name" value="probable transcription factor PosF21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044759">
        <property type="entry name" value="bZIP_RF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13690:SF129">
        <property type="entry name" value="BASIC-LEUCINE ZIPPER DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13690">
        <property type="entry name" value="TRANSCRIPTION FACTOR POSF21-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="8">
        <location>
            <begin position="96"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="44"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="114"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7738688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="292" mass="32830" checksum="EFA87FB07C3397F7" modified="2024-05-29" version="1">MNFRLPDDLDLSSDPYDAPSGSFEELGSEEDLFCSYMEINNLGSSFNNGGETDNGGDGRYRHRHSNSVDNSSLLSESIEAKKAMAPEQLAELWNVDPKRAKRILANRQSATRSKERKARYMSELERKVQTLQTEATTLSAQLTLFQRDTTGLSSENTELKFRLHAMEQQAQLSDALNEALKQELQRLGTATGEIASCSDGYNFGMHQFPYNQSNFFINHQQPEPQHYHHLHQSSMSNHRHLLAAAHTHPFSETLQQDPLGRFQGLDISARTNLHLVKSKGPSISANESSTTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GGF2</accession>
    <name>A0AAD5GGF2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SCP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_025649</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Probably involved in the defense reaction of plants against pathogens.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008157">
        <property type="protein sequence ID" value="KAI7741610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GGF2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05381">
        <property type="entry name" value="CAP_PR-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.33.10:FF:000004">
        <property type="entry name" value="CAP, cysteine-rich secretory protein, antigen 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018244">
        <property type="entry name" value="Allrgn_V5/Tpx1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001283">
        <property type="entry name" value="CRISP-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002413">
        <property type="entry name" value="V5_allergen-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10334">
        <property type="entry name" value="CYSTEINE-RICH SECRETORY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00838">
        <property type="entry name" value="V5ALLERGEN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00837">
        <property type="entry name" value="V5TPXLIKE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00198">
        <property type="entry name" value="SCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="PR-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01009">
        <property type="entry name" value="CRISP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01010">
        <property type="entry name" value="CRISP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0568">Pathogenesis-related protein</keyword>
    <keyword evidence="2" id="KW-0611">Plant defense</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="domain" description="SCP" evidence="4">
        <location>
            <begin position="25"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7741610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="164" mass="18851" checksum="088B4B47EEA238B4" modified="2024-05-29" version="1">MGFQHYWQLVCVVFIYTLHFIYAHSAPHDYVKAHNVVRKEVGLAPVKWNTSIAKFAESYAHKRKSDCALVHSRNKMYGENIALGYGPNGEFSGLDAVKLWVDEKAYYNYESNSCRPSKMCGHYTQIVWKKSTHIGCGRAKCQNNAYFVTCNYYPPGNYIGEKPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GH85</accession>
    <name>A0AAD5GH85_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Expansin-like EG45 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_025755</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008475">
        <property type="protein sequence ID" value="KAI7740276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GH85"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009627">
        <property type="term" value="P:systemic acquired resistance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22269">
        <property type="entry name" value="DPBB_EG45-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="entry name" value="RlpA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044206">
        <property type="entry name" value="EGC1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007112">
        <property type="entry name" value="Expansin/allergen_DPBB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009009">
        <property type="entry name" value="RlpA-like_DPBB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47295">
        <property type="entry name" value="EG45-LIKE DOMAIN CONTAINING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47295:SF7">
        <property type="entry name" value="RLPA-LIKE DOUBLE-PSI BETA-BARREL DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03330">
        <property type="entry name" value="DPBB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="Barwin-like endoglucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50842">
        <property type="entry name" value="EXPANSIN_EG45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042181880" description="Expansin-like EG45 domain-containing protein" evidence="1">
        <location>
            <begin position="24"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="Expansin-like EG45" evidence="2">
        <location>
            <begin position="56"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7740276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="130" mass="13821" checksum="E5F2440D9E05A641" modified="2024-05-29" version="1">MGVLTKALVLISMVVCLTSVAHALSGQAIVSSPPYTPSTCYGSRYQGVMIAKQHISMLANGKACGRRYQVRCLSGTNKAIRNACTGNTVDVKVIGSCQKCGPNELVLSQDAFAKIANVNLGRVNINYEQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GHA4</accession>
    <name>A0AAD5GHA4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PWI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_011938</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007955">
        <property type="protein sequence ID" value="KAI7742600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GHA4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048024">
        <property type="term" value="P:regulation of mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1390.10">
        <property type="entry name" value="PWI domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002483">
        <property type="entry name" value="PWI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036483">
        <property type="entry name" value="PWI_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052225">
        <property type="entry name" value="Ser/Arg_repetitive_matrix"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23148">
        <property type="entry name" value="SERINE/ARGININE REGULATED NUCLEAR MATRIX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23148:SF0">
        <property type="entry name" value="SERINE_ARGININE REPETITIVE MATRIX PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01480">
        <property type="entry name" value="PWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00311">
        <property type="entry name" value="PWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101233">
        <property type="entry name" value="PWI domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51025">
        <property type="entry name" value="PWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PWI" evidence="3">
        <location>
            <begin position="27"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="127"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="127"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="189"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7742600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="264" mass="30371" checksum="5D6828260B7D1044" modified="2024-05-29" version="1">MSGGFFRGTTADQDTRFSNKHAKLLKSHKFPPELEHLVDMSKVKMDVMRPWIAHRVTELLGFEDEVLINFIYGLLEEKVVNGKEIQISLTGFMEKNTSKFMKELWIHLLSAQQNASGVPQQFLDAKEEETRKKLEDTDRINRELKKAKEKESRELGQERGKMAREAVDVKAANLETHSRRRTKFSSKWPADEKGTDETNGSKETARMSRSPQPADRSMSPPRYLIFIIIESFFKEVHGQDQLANPFLIPEVIQGQEVCQLPLKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GJ11</accession>
    <name>A0AAD5GJ11_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_008819</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007524">
        <property type="protein sequence ID" value="KAI7744492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GJ11"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7744492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="59" mass="7130" checksum="C2B5473E338AB559" modified="2024-05-29" version="1">MEKDPEYMGQQRLPQNPFRINWILEEDEQSKKKNQGELNDRTSRCFVHDFIPWPLSPVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GJC3</accession>
    <name>A0AAD5GJC3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">40S ribosomal protein S12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_012021</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008019">
        <property type="protein sequence ID" value="KAI7742331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GJC3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000530">
        <property type="entry name" value="Ribosomal_eS12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11843">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00972">
        <property type="entry name" value="RIBSOMALS12E"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="5">
        <location>
            <begin position="4"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7742331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="111" mass="11852" checksum="679556A40719E6F4" modified="2024-05-29" version="1">MAGKSLAHGGLVRGLHEAAKVIEKHAALLCVLAEDCNQPDYQKLVKALCADHNVSLVTVPAAKTLGEWAGLCKIDSEGKARKVVGCSCLVVKDYGEESEGLHIVQEYVKSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GKW7</accession>
    <name>A0AAD5GKW7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_012768</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006906">
        <property type="protein sequence ID" value="KAI7746725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GKW7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010268">
        <property type="term" value="P:brassinosteroid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016131">
        <property type="term" value="P:brassinosteroid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000029">
        <property type="entry name" value="Cytochrome P450 734A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050665">
        <property type="entry name" value="Cytochrome_P450_Monooxygen"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24282:SF224">
        <property type="entry name" value="CYTOCHROME P450 734A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24282">
        <property type="entry name" value="CYTOCHROME P450 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11 12" id="KW-0349">Heme</keyword>
    <keyword evidence="8 11 12" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 12" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="7 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="binding site" description="axial binding residue" evidence="11">
        <location>
            <position position="378"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7746725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="440" mass="50266" checksum="E761AEAEB282C4D9" modified="2024-05-29" version="1" fragment="single">MDFVDVNNVGPMFLVWFGPMPRLTLADPDLIREVFCLKPELYEKNEAHPLIKQLEGDGLLNLKGEKWAYHRKIITPTFHMDNVKLLVPVLTGSVMKMLDKWLSMSDFGEVEIEVSQWYQNLIMDTMTRAAFGNNHIDGKRIFQLQARQMVLTSEAFKQIPIPGYRLLPTKRNIESWKLHKEINNSLVRIIERRRNWGDIDMVNGPKDLLGLMIEASRKESSPVITTREIAEECKNIFFAGEQTTSNLLTWTTVLLAMHPQWQVIARDEVLNVFGPRDIPTKDCVSKLKTLTMILNESLRLYPPIVASIRRAKAEVELGGYKIPRGSELLIPILAIHHDQSIWGNDANEFNPSRFSNGVARAAKHPMAFFPFGLGVRTCIGQNLAILQAKLTIAMILQRFSFELSPKYQHAPTVLIMLHPQYGAPITFKRLRTDPSTKEIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GL27</accession>
    <name>A0AAD5GL27_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">U1 small nuclear ribonucleoprotein 70 kDa</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_018129</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus speckle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006879">
        <property type="protein sequence ID" value="KAI7746907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GL27"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030619">
        <property type="term" value="F:U1 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:001585">
        <property type="entry name" value="U1 small nuclear ribonucleoprotein 70 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051183">
        <property type="entry name" value="U1_U11-U12_snRNP_70-35kDa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022023">
        <property type="entry name" value="U1snRNP70_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13952">
        <property type="entry name" value="U1 SMALL NUCLEAR RIBONUCLEOPROTEIN 70 KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13952:SF5">
        <property type="entry name" value="U1 SMALL NUCLEAR RIBONUCLEOPROTEIN 70 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12220">
        <property type="entry name" value="U1snRNP70_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="258"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="343"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="365"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="382"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="432"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="551"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="579"/>
            <end position="602"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7746907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="602" mass="71228" checksum="6E027ED7ABCC45AC" modified="2024-05-29" version="1">MGDYNNDPFMRGGANNNAAAVQARTKQQNRANLMQMKLIGQSHPTGLTNNLLKLFEPRPPLEYKPPPEKRKCPPYTGMAQFVSNFAEPADPEYAPPIQKGETPGQKKARIHQLRLEEGAKKAAEELEKYDPNSDPNISGDPYKTLFVARLNYETTESRIKRDLSVYKILISYDDKFGLPTVTSFIWNLDFKHIYHMISEKLNLRYCKCNFSLLMRLHVLLPTTSPLPTLLVSFPCFISLIKSQLQDFEILGILVERVATIQLVSFKYLHLLSKTFQKLRRFSVPGKGRVRLIADKESNKPRGYAFIEYMHTRDMKAAYKQADGRKIDNRRVLVDVERGRTVPNWRPRRLGGGLGTSRVGGEEVNQRVSGREQSQSGGPTRSVEPRATEERDRDREKSRERVKEREREKSRERSHDRPRDREHREDRHHRDRDRKSDRDRDRSGRDRDRHRDRDRDRSGRDRSGRDRDRYRDKDREGDVEHMDRDHEHDRGRSRDKEYEYDRVDRHERDKHGDRQKSYDRGDPEEMYDQPVHGNAKPADYERDPGGYDDYYDQERYEHQDRDHDGYYQMEEDNYEPGQADPHDKDYDRSYRQSDRSLSREYEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GNN5</accession>
    <name>A0AAD5GNN5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Beta-ketoacyl-[acyl-carrier-protein] synthase III</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_014006</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006318">
        <property type="protein sequence ID" value="KAI7749637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GNN5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004315">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00830">
        <property type="entry name" value="KAS_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013747">
        <property type="entry name" value="ACP_syn_III_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013751">
        <property type="entry name" value="ACP_syn_III_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006829">
        <property type="entry name" value="PRK09352.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43091">
        <property type="entry name" value="3-OXOACYL-[ACYL-CARRIER-PROTEIN] SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43091:SF4">
        <property type="entry name" value="BETA-KETOACYL-[ACYL-CARRIER-PROTEIN] SYNTHASE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08545">
        <property type="entry name" value="ACP_syn_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08541">
        <property type="entry name" value="ACP_syn_III_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Beta-ketoacyl-[acyl-carrier-protein] synthase III N-terminal" evidence="8">
        <location>
            <begin position="42"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-ketoacyl-[acyl-carrier-protein] synthase III C-terminal" evidence="7">
        <location>
            <begin position="183"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7749637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="273" mass="29161" checksum="36BC7E7112450E39" modified="2024-05-29" version="1">MAEVDVDDVDLILFCSSTPDDLFGGAPEVQKNLGCKRNPPAFDIRAACSGFLLGLVSASCHIRGGGFKNVLVIGADCISHYVDWKDRKTCVLFGDAAGAILLQACDSEDDGLYGFDLHTDGYGSRKLNAGIKHMTETDFALGSNADALGFSPTHASISYIQMNGQEVFRFVVKAVPESIMASLANSGLSLSDIDWLLVHQANQRIIDYVATKLEFPTDRVISNLKNYGNTSAASIPLALDEAVRSGKVKKGQTVMTAGFGAGLTWGSAVVRWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GPU6</accession>
    <name>A0AAD5GPU6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">acylaminoacyl-peptidase</fullName>
            <ecNumber evidence="5">3.4.19.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_015238</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleavage of an N-acetyl or N-formyl amino acid from the N-terminus of a polypeptide.</text>
            <dbReference type="EC" id="3.4.19.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase S9C family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.1" evidence="5"/>
    <dbReference type="EMBL" id="JAMZMK010006734">
        <property type="protein sequence ID" value="KAI7747553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GPU6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008242">
        <property type="term" value="F:omega peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000146">
        <property type="entry name" value="Acylamino-acid-releasing enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045550">
        <property type="entry name" value="AARE_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42776:SF4">
        <property type="entry name" value="ACYLAMINO-ACID-RELEASING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42776">
        <property type="entry name" value="SERINE PEPTIDASE S9 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19283">
        <property type="entry name" value="APEH_N"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acylamino-acid-releasing enzyme N-terminal" evidence="9">
        <location>
            <begin position="64"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="Acylamino-acid-releasing enzyme N-terminal" evidence="9">
        <location>
            <begin position="348"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="domain" description="Acylamino-acid-releasing enzyme N-terminal" evidence="9">
        <location>
            <begin position="500"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="8">
        <location>
            <begin position="612"/>
            <end position="806"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF19283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7747553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="807" mass="88279" checksum="A97DDD385E0CCE45" modified="2024-05-29" version="1">MLLQRYLTNTSFSTCSAFNPHLLLLHFPKPHPHPSSSSSLLRFSSKRFGVCSVMDGSGVSAVKEVAPNLDANMEEEYVSQSKLYKEFASMSTIEKAWTFKSSSGRGSHTMFTISQPNLLANKKKKLMLSSVISENSNGGVDVEWAPFPIEVTGASLMVPSPSGSKLLVVRNPENDSPTLFEIWGPFQLEKEIRVPQSKHGSVYADGWFQGISWSSDESAIAYVAEEPSVQKPSFSDTAGYRKDIGSNSTDKDLNSWKGQGDWEEDWGEAYSGKRQPALFVKTPTLFLLGAKDLRVPVSNGLQFARALKEKGVPVKVIVFPEDIHPINRPQSDFESFVNVGVWFKKYCNGDVSAVQGIGRSLSVGQVVWAPTTKGVHEYLIFVGWPSDTRKFGMIYCYNRPCAIYAVKAPSFGSETKDNAVNDDASVINLTQGISSAFLPRFTPDGKFLVFLSAKTCVDTGAHSATQSIHKIEWNNEGEPSSAKIVDVWESNKNHSQQFKSFMDSLVEVPQIKYGSITKEESKDDIWRWLDVSSPTSESQEKVKSLLSSLQLDILKIPVNDVSKSLTKGACKPIEAMYVSSKSKHQDCDPMIVILHGGPHGISLSSFSKTSGGSIGYGEEALQSLLGKVGSQDVNDVLTAVDHAVDMGLANPSKITVLGGSHGGFLTTHLIGQAPDKFAAAVARNPVCNLALMVGTSDIPDWCFVETFGTQGLSSYTEAPSLQLVSSFHEKSPISHISKVKTPTLFLLGAKDLRVPVSNGLQFARALKEKGVPVKVIVFPEDIHPINRPQSDFESFVNVGVWFKKYCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GQ14</accession>
    <name>A0AAD5GQ14_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Alkaline/neutral invertase</fullName>
            <ecNumber evidence="6">3.2.1.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_003090</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Invertase that cleaves sucrose into glucose and fructose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides.</text>
            <dbReference type="EC" id="3.2.1.26"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the glycosyl hydrolase 100 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.26" evidence="6"/>
    <dbReference type="EMBL" id="JAMZMK010006058">
        <property type="protein sequence ID" value="KAI7750925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GQ14"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033926">
        <property type="term" value="F:endo-alpha-N-acetylgalactosaminidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004575">
        <property type="term" value="F:sucrose alpha-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005987">
        <property type="term" value="P:sucrose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000001">
        <property type="entry name" value="probable alkaline/neutral invertase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024746">
        <property type="entry name" value="Glyco_hydro_100"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31916">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31916:SF15">
        <property type="entry name" value="ALKALINE_NEUTRAL INVERTASE D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12899">
        <property type="entry name" value="Glyco_hydro_100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5 6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7750925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="552" mass="63014" checksum="4E89B121581E3176" modified="2024-05-29" version="1">MDATREAGLRNVSSVCSIPEMDDFDLTKLLDKPRLTIKRERSFDERSLSEMSISRGLDNLELAYSPGGRSGLDTPGSSTRNSFEPHPMVAEAWEALRRSLVHFRGQPVGTIAAYDHASEEVLNYDQVFVRDFVPSALAFLMNGEPDIVKNFLLKTLQLQGWEKRIDKFRLGEGAMPASFKVLHDPERKTDTIVADFGESAIGRVAPVDSGFWWIILLRAYTKSTGDLSLAETPDCQKGMRLILTLCLSEGFDTFPTLLCADGCSMIDRRMGIYGYPIEIQALFFMALRCSLTMLKHDTEGKEFMERIMKRLHALSFHMRSYFWIDFQQLNDIYRYKTEEYSHTAVNKFNVIPDSIPDWVFDFMPTRGGYFIGNVSPARMDFRWFALGNCVAILSSLATPEQASAIMDLFEARWEELVGEMPIKICYPAIESHEWRIVTGCDPKNTRWSYHNGGSWPVLLWLLTAACIKTGRPQIARRAIELAESRLLKDGWPEYYDGKLGRYIGKQARKYQTWSIAGYLVAKMMLEDPSHSGMISLEDDRQLKPIIKRSYSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GQP7</accession>
    <name>A0AAD5GQP7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">CSC1-like protein At4g02900</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_021439</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CSC1 (TC 1.A.17) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005874">
        <property type="protein sequence ID" value="KAI7751555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GQP7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005227">
        <property type="term" value="F:calcium-activated cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045122">
        <property type="entry name" value="Csc1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003864">
        <property type="entry name" value="CSC1/OSCA1-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027815">
        <property type="entry name" value="CSC1/OSCA1-like_cyt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032880">
        <property type="entry name" value="CSC1/OSCA1-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018:SF96">
        <property type="entry name" value="OS05G0393800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018">
        <property type="entry name" value="PROBABLE MEMBRANE PROTEIN DUF221-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14703">
        <property type="entry name" value="PHM7_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02714">
        <property type="entry name" value="RSN1_7TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13967">
        <property type="entry name" value="RSN1_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="120"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="172"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="389"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="441"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="481"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="588"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="644"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="667"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like N-terminal transmembrane" evidence="12">
        <location>
            <begin position="7"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like cytosolic" evidence="13">
        <location>
            <begin position="214"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like 7TM region" evidence="11">
        <location>
            <begin position="387"/>
            <end position="659"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF13967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF14703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7751555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="779" mass="89301" checksum="9E9D4F693170D673" modified="2024-05-29" version="1">MATLSDIGVSAAINCLSAILFLVAFAILRLQPINDRVYFPKWYIKGIRDSPTSSGAFVKKFVNLDVNMYLKFLNWMPAALRMPEPELIEHAGLDSAVYIRIYLLGLEWNDEIAVNFSLKIFFPITLLSFAVLVPVNYTGGNFEAMKTNTTDLTYSDIDKLSISNVAPGSSRLYAHIGMAYVFTFWTCYTLYREYMIVAKMRLHFIAAERRRPDQFTVLVRNVPPDADESVSEHVDHFFCVNHPDHYLLHQVVYNAITLADMVLEKKTLQNWLTYYTNKHERHPNKRPTTKTGFWGLWGRNVDAIDFYREQIETLSKKEEAERERVLNDPNAIMPAAFVSFRSRWGAAVCAQTDQSHNPTKWLTQWAPEPRDVYWDNLAIPYVELKLRRLLMAVALFGLTFCFMVPIAFVQTLANIEGIQKVLPFLKPLIQMGSVKSLIQGYLPGIILKIFLILLPTIIMTMSKIEGFTAHSALETRSAEKYHLFLLVNVFLGSIITGTALQQLKQFMNQSPTEIPKTVGVSIPMKATFFITYVMVDGWSGVAAEILRLVPLIVFHLKNAFLVKTDEDREEAMDAGSLTWSVSEPRIQLYFLLGLVYSTVTPILLPFIVVFFAFAYLVFRHQIINVYCQKYESGATFWPDVHRKIIIGLLISQFLFLGLLNTKKATNATFFLIPLPILTFWFFKVCKGRFESQFRRFPLEDAMKKDTLERATEPNLNLKLYLQDAYVHPVFKGSTEFERLRVADEEENSQLVATRRIVHRRGNQEVQQDSSEDIMGISAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GSG4</accession>
    <name>A0AAD5GSG4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Clathrin/coatomer adaptor adaptin-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_033742</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006155">
        <property type="protein sequence ID" value="KAI7750418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GSG4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030126">
        <property type="term" value="C:COPI vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005793">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000078">
        <property type="entry name" value="Coatomer subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002553">
        <property type="entry name" value="Clathrin/coatomer_adapt-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017106">
        <property type="entry name" value="Coatomer_gsu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10261">
        <property type="entry name" value="COATOMER SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10261:SF0">
        <property type="entry name" value="COATOMER SUBUNIT GAMMA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01602">
        <property type="entry name" value="Adaptin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Clathrin/coatomer adaptor adaptin-like N-terminal" evidence="1">
        <location>
            <begin position="25"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7750418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="382" mass="42911" checksum="CB915F1302D73716" modified="2024-05-29" version="1" fragment="single">MAQFVKKDDDRDEEEYSPFLGIEKGAVLQEARVFNDPQLDSRRCSQVITKLLYLLNQGETFTKVEATEVFFSVTKLFQSKDIGLRRMVYLIIKELSPSSDEVIIVTSSLMKDMNSKTDVYRANAIRVLCRITDGTLLTQIERYLKQAIVDKNPVVASAALVSGIHLLQTNPEIVKRWSNEVQEAVQSRAALVQFHGLALLHQIRQNDRLAVSKLVTSLTRGTVRSPLAQCLLIRYTSQVIRESSMNNQSGERPFYDYLEGCLRHKAEMVIFEAARAITELNGVTSRELTPAITVLQLFLSSSKPVLRFAAVRTLNKVAMTHPMAVTSCNIDMESLISDQNRSIATLAITTLLKTGNESGVDRLMKQITNFMSDIADEFKIVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GV16</accession>
    <name>A0AAD5GV16_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_015622</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000430">
        <property type="protein sequence ID" value="KAI7756355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GV16"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="18"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7756355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="186" mass="21017" checksum="979E25B2F0FCF640" modified="2024-05-29" version="1" fragment="single">MNIPVVLSKFMFDEPSGWVTMATVVTLLSTIYLGFAEIIGSHLQYSKFTNNSKHDDDGMMKVSSRRGMLILYTPAFLAGFVSFFVFPGGGLRFLFLKLALTFHFFKRDFEGFGTVYGGDPLWRMNRLSLTIGEQDRETEIQEFLGRAATIGAFVKRGEEAGYTKITLKGKTPEEKIVIMRKIDARN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GVY6</accession>
    <name>A0AAD5GVY6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">M8C21_000272</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">AA19_3_7</strain>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Required for vesicular transport from the ER to the Golgi complex. Functions as a SNARE associated with ER-derived vesicles.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the BET1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000074">
        <property type="protein sequence ID" value="KAI7757722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GVY6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15841">
        <property type="entry name" value="SNARE_Qc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000056">
        <property type="entry name" value="Bet1-like protein At4g14600"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12791">
        <property type="entry name" value="GOLGI SNARE BET1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="109"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="16">
        <location>
            <begin position="39"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="42"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00060029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAI7757722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="133" mass="14987" checksum="4D5C3D9F6D87926E" modified="2024-05-29" version="1">MAASSSRGGGYYGAAPYRSREGLSTRQGTSSDEIQLRIDPMHADLDDEISGLRKQVRQLRNVASEIETEAKFQNEFINQLQMTLIKAQAGVKNNMRKLNKSIVQSGSSHVLHVVLFALFCLFLVYLYSKFSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GWJ4</accession>
    <name>A0AAD5GWJ4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_026672</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010002287">
        <property type="protein sequence ID" value="KAI7754886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GWJ4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16897:SF22">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16897">
        <property type="entry name" value="OS10G0105400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="296"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="397"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="509"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="633"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="698"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="919"/>
            <end position="941"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="297"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="309"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="397"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="427"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="455"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="562"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="705"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="716"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="729"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="752"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="927"/>
            <end position="938"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7754886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="1001" mass="112710" checksum="079091E7FA3BF89C" modified="2024-05-29" version="1">MAIGGNDDVQDPSVHPWGGLTTTRDGTLTLLDCYLCSKYLKGLQNVFDSARARERERELLYPDACGGGGRGWISQGMVGYGRGHGTRETCALHTARLSVDTLVDFWSALGEETRYSLLRMKEEDFMERLMYRFDSKRFCRDCRKNVIREFKELKELKRMRKETRCTGWFCAADTSFQYEVTNDTIHAEWHQNYADSKGIYEYYEWAVGTSEGKSDILDFENVGSKSRVQVNGLDLTGLNSCYITLRAWRTDGRCNEVSVKAHALKGQHCVHGRLVVGDGFVTITRGESIRRFFEHAEEAEEEEEDDSVDKDGNDLDGECSRPQKHAKSPELAREFLLDAATVIFKEQVEKAFREGTARQNAHSMFVSLALKLLEQRVIVACKDIITLEKQFKLLEEEEKEKRDEEERKERRRAKEKEKKLRRKERLRIKEQEKEKKCGQLSQNPIPDDATKEELTSVNNEPEINDNSYCEELEDTQSSMLASDDYAQEDQILEYDENSNVEYDYQKDENQLFVNDQSKHNRRRPKSWKDYQYDQPSKWSDRRRSEYGSTVNKPVPRYHSDGYETSSRNGNLNGVNKSVSRSNGSRYTDRSHCCHSRTSGKYDPPACNCYQHTYYRPKSGIPEPDIDVSKPYFRRNKYSNSNQTESVRERPKTKIVSGNNGLGYIKKVWEPRCNLDSDDVTDQSADAISPVKLVNLPNETNVNASDLKDSDMKESKSSVQIETEPESGTTDLSTSSSNSNSDSCSSCLIEGDGSTSLSSTPQNTESSTSDSECANHQSEVTKETSSCLENADELLSKTSESVTGLCSQGNNVALPPLQAQSIHFPVFQAPPSMGYYHQGPVPWTSGPASGIMPLGTLPHPNHYLFASPFGYGNSHYVPYSVGGLQPPLLNHGQLPMYPAVPSVNGVKDPNTKAIPIKGEQVVNSEKPQNGKHENSEKTQKGSTGFSLFHFGGPVDVSNGFNPDAADCVKEETEVNVDSSCVVKDVEEYNLFAASNGIQFSIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GY14</accession>
    <name>A0AAD5GY14_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SHSP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_014927</name>
        <name evidence="5" type="ORF">M8C21_015134</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008920">
        <property type="protein sequence ID" value="KAI7737941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAMZMK010000816">
        <property type="protein sequence ID" value="KAI7755803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GY14"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031107">
        <property type="entry name" value="Small_HSP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11527">
        <property type="entry name" value="HEAT-SHOCK PROTEIN 20 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="SHSP" evidence="4">
        <location>
            <begin position="19"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7737941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7755803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="137" mass="15687" checksum="85C8319FC9FF2E93" modified="2024-05-29" version="1">MEFSTFNPSWHSFFTSPLLFPYPFVPENYVHWTETPESHIYSADIPGVRKEEICVEVEDSRYLIIRTESADDTVTMPGGRTFMKKFRLPDTIDVNGISACYENGVLTVTVPRSFVRRGFYIEPADLPQQTEVLARAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BLE8</accession>
    <name>A0AAD5BLE8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Aspartate aminotransferase</fullName>
            <ecNumber evidence="8">2.6.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_025525</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:21824"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="EC" id="2.6.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3 8">Homodimer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="8">In eukaryotes there are cytoplasmic, mitochondrial and chloroplastic isozymes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.1" evidence="8"/>
    <dbReference type="EMBL" id="JAMZMK010011920">
        <property type="protein sequence ID" value="KAI7725542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BLE8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004069">
        <property type="term" value="F:L-aspartate:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000052">
        <property type="entry name" value="Aspartate aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000001">
        <property type="entry name" value="Aspartate aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000796">
        <property type="entry name" value="Asp_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006719">
        <property type="entry name" value="PRK09257.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11879">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11879:SF22">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00799">
        <property type="entry name" value="TRANSAMINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="9">
        <location>
            <begin position="12"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7725542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="382" mass="41735" checksum="507DAB2EF7204B61" modified="2024-05-29" version="1" fragment="single">VTVAYNKDSDPSKLNLGVGAYRTEEGKPLVLNVVRQAEQKLVNDPSRVKEYLPIVGLADFNKGSAKLIFGADSPAIQENRVATVQCLSGTGSLRVGGEFLAKHYYEKTVYIPSPTWGNHPKIFTLAGLSVKTYRYYDPATRGLDFQGLLEDLGNAPTGAIVLLHACAHNPTGVDPTLDQWEQIRKLMRSKGLLPFFDSAYQGFASGSLDADAQSVRMFVADGAQSYAKNMGLYAERVGALSIVCKTADVASRVESQLKLVIRPMYSNPPLHGASIVATILNDSNLYNEWTVELKAMADRIISMRKQLLEALQAKGTPGDWTHIIKQIGMFTFTGLNAEQVAFMTREYHIYMTSDGRISMAGLSSKTVPHLADAMHAAVTRVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BM44</accession>
    <name>A0AAD5BM44_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Xyloglucan endotransglucosylase/hydrolase</fullName>
            <ecNumber evidence="8">2.4.1.207</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_004591</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Secreted</location>
            <location evidence="8">Cell wall</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Secreted</location>
            <location evidence="8">Extracellular space</location>
            <location evidence="8">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="8">Contains at least one intrachain disulfide bond essential for its enzymatic activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the glycosyl hydrolase 16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.207" evidence="8"/>
    <dbReference type="EMBL" id="JAMZMK010012171">
        <property type="protein sequence ID" value="KAI7724714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BM44"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016762">
        <property type="term" value="F:xyloglucan:xyloglucosyl transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042546">
        <property type="term" value="P:cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010411">
        <property type="term" value="P:xyloglucan metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02176">
        <property type="entry name" value="GH16_XET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000025">
        <property type="entry name" value="Xyloglucan endotransglucosylase/hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000757">
        <property type="entry name" value="Beta-glucanase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044791">
        <property type="entry name" value="Beta-glucanase/XTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008263">
        <property type="entry name" value="GH16_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010713">
        <property type="entry name" value="XET_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016455">
        <property type="entry name" value="XTH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31062">
        <property type="entry name" value="XYLOGLUCAN ENDOTRANSGLUCOSYLASE/HYDROLASE PROTEIN 8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00722">
        <property type="entry name" value="Glyco_hydro_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06955">
        <property type="entry name" value="XET_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005604">
        <property type="entry name" value="XET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01034">
        <property type="entry name" value="GH16_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51762">
        <property type="entry name" value="GH16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0052">Apoplast</keyword>
    <keyword evidence="8" id="KW-0134">Cell wall</keyword>
    <keyword evidence="8" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="1 8" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041765781" description="Xyloglucan endotransglucosylase/hydrolase" evidence="8">
        <location>
            <begin position="29"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="GH16" evidence="9">
        <location>
            <begin position="30"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="113"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="6">
        <location>
            <position position="117"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="7">
        <location>
            <position position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR005604-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR005604-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7724714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="300" mass="34900" checksum="44EC996DF50DC4FD" modified="2024-05-29" version="1">MSRTQIWGGGHMALMCVCMLATAHFVIGAAPKKPVAVPFGRNYVPTWAFDHIKYFNGGSEIQLLLDNYTGTGFQSKGSYLFGHFSMQIKMVPGDSAGTVTAFYLSSQNNEHDEIDFEFLGNRTGQPYILQTNVFTGGKGDREQRIYLWFDPTKAYHSYSVLWNLHQIVFLVDDVPIRVFKNSKDLGVRFPFNQPMKIYSSLWNADDWATRGGLEKTDWSKGPFLAHYKNFHVDGCESSVNAKYCETQGKRWWDQKEYQDLDAYQWRRLRWVRKKFTIYNYCTDRKRFPTMAPECKRDRDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BMQ9</accession>
    <name>A0AAD5BMQ9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">RNA helicase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_010603</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DEAD box helicase family. DDX21/DDX50 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011723">
        <property type="protein sequence ID" value="KAI7726273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00268">
        <property type="entry name" value="DEADc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12938">
        <property type="entry name" value="GUCT_Hera"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001060">
        <property type="entry name" value="ATP-dependent RNA helicase RhlE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044742">
        <property type="entry name" value="DEAD/DEAH_RhlB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012562">
        <property type="entry name" value="GUCT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF1">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08152">
        <property type="entry name" value="GUCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="142"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="8">
        <location>
            <begin position="545"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="78"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="440"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="78"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="460"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7726273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="564" mass="60690" checksum="E93CF96A74DAF987" modified="2024-05-29" version="1">MACSSSIINISSIFHKNPSLEHLSRKLLTXXXXXSSNSKPNQTASGFVTSATATPNSILSDKAFKGLGVFDEDDDDVLDGSDRDYDNDDDVSEGEVLGDADGSSVGDDDELLITKLGLPQRLVKSLEKREITRLFPIQRAVLVPALEGRDIIARAKTGTGKTLAFGIPIIKRLTEDDEERIAPRRGQRLPRVLVLAPTRELARQVETEIKESAPYLSTVCVYGGVSYSSQQNALSRGVDVVVGTPGRLIDLIGSNSLKLGEVQFLVLDEADQMLAVGFEEDVEHILEKLPSERQSMLFSATMPGWVKKLSRKYLNNPLTIDLGAGALAAALAQLSGFSKPPSSRSLISHEQGWTTLQLTRDPALSRGFMSARTVTGFLADVYATAADELGKIHVIADERVQGAVFDLPEEIAKELLNKDLPPGNTITKITKLPALQDDGPAGDFYGRFSNRERSSRGAGSRRGGSRGGWGGSSRFSSDDEGDSFRRGGRSGGSSWSRGGSRSGGGGGSDWLIGERRSSRSPSFGTRGSGSGSMSRSRDRNFDGACFHCGRSGHRASECPKKQDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BMV8</accession>
    <name>A0AAD5BMV8_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_008115</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011711">
        <property type="protein sequence ID" value="KAI7726317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BMV8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015020">
        <property type="term" value="F:glucuronosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044610">
        <property type="entry name" value="GLCAT14A/B/C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003406">
        <property type="entry name" value="Glyco_trans_14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45719:SF5">
        <property type="entry name" value="BETA-GLUCURONOSYLTRANSFERASE GLCAT14B-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45719">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02485">
        <property type="entry name" value="Branch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7726317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="258" mass="29672" checksum="DA8043AC5BA7A7AF" modified="2024-05-29" version="1">MVVGCNYKGTGLVWCTKAAWLCMDLLHTFSTVPRDLNFIEHTSDIGWKEYQRAKPVIIDPGLYSLKKSDVFWVSQKRSVPTAYKLFTGSAWMMLSRPFVEYCLWGWDNLPRIVLMYYANFLSSPEGYFHTVICNAEEFKNTTVNHDLHFISWDNPPKQHPHFLSIDDYDRMVESHAPFARKFGSDKELLDKIDTELLGREPDGFVTSNWLDRSDANKTIAESIVRNATELKPGPGAERIKTLIGGMLSDKDFHLKHCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BMW3</accession>
    <name>A0AAD5BMW3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Leucine-rich repeat-containing N-terminal plant-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_029679</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RLP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011975">
        <property type="protein sequence ID" value="KAI7725331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BMW3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000129">
        <property type="entry name" value="Leucine-rich repeat receptor-like kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000041">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000095">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001347">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046956">
        <property type="entry name" value="RLP23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063:SF106">
        <property type="entry name" value="LEUCINE-RICH REPEAT DOMAIN, L DOMAIN-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063">
        <property type="entry name" value="LRR RECEPTOR-LIKE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="726"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="13">
        <location>
            <begin position="19"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="784"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7725331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="784" mass="87419" checksum="D74A72FDE3B75C15" modified="2024-05-29" version="1" fragment="single">FGTSSNAIITTSSNITCLERERQSLLVFKRNLTDTYDLLSTWSGLECCEWHGVGCDSRDGHVVKLDLHNKWLTGELSPSLQNLKHLRYLDLSLNNISGYIPEFLGSFQYLEYLNFSGSSFVGVVPHNLGHLSRLQYLDLSNVESLKLDDLGWLSSLSSLRYLDLSTITIGKHIDWFRPVNMLPSLLTLNLAYCGINIPSTNFANLTSLSSLDLSFNELNFTIPAWLSNLTSLMHLNLEFNHFHGQIPIFFGTFSALASIDLSSNSLETSMPVLLCNLSSLVRLDLSWNMFSGPIPANLGLLLKLEDINFQYNRLSGYIPTSLGQLSNLKKLDLSHNSLVGVLSETHFTKLENLNYLVLSSNSLALNLSSRWIAPFQLQIFSASSCNIGPHFPNWLQTQTNLQRLTLSNSSIRDTIPEWFENTLSHILYLDLSNNQIGGKLPRLHFNSSSQIGGGILKMNSNKFEGSVAMFPSNVKLLDLSDNLLSVDILHTFRKINSSLEVVNLSKNRFTGSIPVHLCKFPSIYVLDLSHNKFSGMLPGCLGNLTNLSVLDVSNNTITGVVPSSLGSLKGLISLHLHNNIFEGDIPVSLQNLTQLMTMDLGNNLFMGAIPLWIGEKLSNLRILNLQSNKFIGKIPRRLCQLSALQYLSLAHNNINGTIPDCFRNLSGMITASREYIVLNYNENILASIKGMELLYTNTIHCNVNNVARNHVGENGEDEGQDDTEGLWFYVSMGLGFVVGFTGLLGSQFIRSWRIAYFETLENVYGWLTATIQKNLAGLRRKLSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BP68</accession>
    <name>A0AAD5BP68_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_000269</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011602">
        <property type="protein sequence ID" value="KAI7726704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BP68"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36034">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36034:SF2">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="177"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="358"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="697"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="11"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="177"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7726704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="805" mass="88142" checksum="AAA983764A064402" modified="2024-05-29" version="1">MNFLGLRSHSQVPQALPPQVQAEATTRKPTATLEELVAQDPFPFVEPFDVTLTENDALFATNNVVVAAAEVDEEEEDKHIDVSQEQGWITIPCKELPDRWNEEPDMQSFKSFDRPFVFPGEQVHILVCLSAYKQDTEVITPFKVAELMSKNGIGHAKKQNGEVIDGSPLAHEVASSTVVQDSDDNANGKTGPKKDVSTGESLLRMEDHRRQTEMLLQRFRNSHFFARIAEADEPLWSKRKYHETFRESTEMIGGKLGEYDDKQILPLDKENSDASTSGGVARNGFKCSSLANGDIVVLLQVNIGVECLKNPVLEIIQFEKYEDRSLSAEGSDLSYSNQQGDPCGDLLKWLLPLENSLASPSRSLSPPQLGSSSSSIRSTSTKMNSLGSSSSPIFSFGHFRSYSMSSVPPSTIPPPVPPTTNSRSILLEDQDHLSSNKLGKDEKSEGEGLVSFRGVPLEPERFSVRCGLQGIYIPGRRWRRKVEIIQPVEITSFTADCNTEDLLCVQIKNVSPAHMPDIVIYLDTITIIHEEASKGGPPLSLPIACIEAGIDHSLPDLALRRGEEHSFILKPATSLWRNSSKGLSPRLSSSVHGPPSDGVMLGDKYAVLVSCRCNYTESRLFFKQPTSWRPRMPRDLLISVASQMSRPTLGSDDRIPQLPVQVLTLEASNLTSEDLTLTVLAPASFSCLPSVVSINSTPASPTDGDSRQDGNGPQTGSFNEYAISITDVLPTSDLGCTHLWLQSRVPLGCVPCRSTATIKLELLPLTDGIITLDSLQISVKEKGLTYVPEKSLKINATSSISSGII</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BQB9</accession>
    <name>A0AAD5BQB9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_003819</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011356">
        <property type="protein sequence ID" value="KAI7727515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BQB9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050648">
        <property type="entry name" value="F-box_LRR-repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13382">
        <property type="entry name" value="MITOCHONDRIAL ATP SYNTHASE COUPLING FACTOR B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13382:SF21">
        <property type="entry name" value="OS12G0601000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="51"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="527"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7727515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="527" mass="57820" checksum="8AB1EB47D924FEE8" modified="2024-05-29" version="1" fragment="single">KPRGSYNCRRCGQPKKGHVCLLPPPPLTTDSAAVTPTVSSTSKPPPSVSSICSIRTMPVSLVWEVMRRLPPATLLTAAKVCTDWRDVIQQLWKAAEEVRVRVPAKTPVGYVTSVLQKCCSNLVRLSLTMESLMVNVNDMDSTMLACIAFACPNLESLAIRMCEGSFNRVTGDELGRFVAEKRCLTTLKMEGCSNLGSFVLCSNILSTLWLSDLHSLSKMVFNTPNLIEISLDFSRQENDSTDLTSMMDGLGRTCPRLQNIHIASLQLSHSVVLSLTAANLRGLRMLSLVLGSEITDASVVAISKCYSNLELLDLSGSSISDTGLGMICNIFPETLTRLLVALCPNITSSGIQFATAQLPLLELMDCGMTICDSDAQPPTSGEKSDSSLHTSPNSKLHLTHQKLFIKHARLKRLSLWGCYSLDSLYVSCPNLNDLNLNSCTNLSPEKLVLQCPKLESVHAIGCEDVLIQAVQTQVSDDFASLENRYSCKRMADGSKRVGIPFSFSQQLPDYDRTSWKIRKRRCTVVVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BS15</accession>
    <name>A0AAD5BS15_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">histidinol-phosphate transaminase</fullName>
            <ecNumber evidence="3">2.6.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Imidazole acetol-phosphate transaminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_005763</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-histidinol phosphate + 2-oxoglutarate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:23744"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57766"/>
            <dbReference type="ChEBI" id="CHEBI:57980"/>
            <dbReference type="EC" id="2.6.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 7/9.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.9" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010011190">
        <property type="protein sequence ID" value="KAI7728611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BS15"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004400">
        <property type="term" value="F:histidinol-phosphate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01023">
        <property type="entry name" value="HisC_aminotrans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005861">
        <property type="entry name" value="HisP_aminotrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01141">
        <property type="entry name" value="hisC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42885:SF2">
        <property type="entry name" value="HISTIDINOL-PHOSPHATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42885">
        <property type="entry name" value="HISTIDINOL-PHOSPHATE AMINOTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="8" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="11">
        <location>
            <begin position="92"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7728611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="419" mass="46856" checksum="601424F1B2372780" modified="2024-05-29" version="1">MGMINGSSVSMIKSNNGFCVSPSLNGKSYLISRYQTKFNNKCMVSCSTSSVEHQLKLTGDSFIRPHLRELSPYQPILPFEVLSTRLGRKPEDIVKLDANENPYGPPPEVFEALGNLKFPYIYPDPESRTLRGALAEDSGLESEYILVGCGADELIDLIMRCVLDPGDSIVDCPPTFTMYEFDAAVNCARVIKVPRKSDFSLDVQRITEVIEHEKPKIIFLTSPNNPDGSVINDEVLLKILDLPILVVLDEAYIEFSGLESKMGWVKKHENLIVLRTFSKRAGLAGLRVGYGAFPLSIIEYLWRAKQPYNVSVAAEVSACAALQNPVYLEKVKVALVQERERLFKLLKQVPFLNPYPSYSNFILCEVTNGKDAKKLKEDLAKMGVMIRHYSNKELKGYVRVSVGKPEQTDALMDCVARLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BSH7</accession>
    <name>A0AAD5BSH7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">M8C21_008777</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">AA19_3_7</strain>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010011199">
        <property type="protein sequence ID" value="KAI7728504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BSH7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009742">
        <property type="term" value="P:brassinosteroid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000016">
        <property type="entry name" value="probable serine/threonine-protein kinase At4g35230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000154">
        <property type="entry name" value="probable serine/threonine-protein kinase At4g35230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000069">
        <property type="entry name" value="probable serine/threonine-protein kinase At5g41260"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045845">
        <property type="entry name" value="BSK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058209">
        <property type="entry name" value="TPR_BSK1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45863:SF8">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45863">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE BSK5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25575">
        <property type="entry name" value="TPR_BSK1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1070">Brassinosteroid signaling pathway</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0519">Myristate</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="55"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="20"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="18">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="18">
        <location>
            <position position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAI7728504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="470" mass="52573" checksum="1BF52BB8D78326A0" modified="2024-05-29" version="1" fragment="single">SKTANVQSPDQVPESKPDPGDGEQVGEQEQDQQVPVFKEYELTELRAATNGFSSELIVSESGEKAPNVVFRGKLRNNKVVAIKRFSKLSWPDPQQFVAEAAGVGKVRHNRLVNLIGCCAEGDERLLVAEYMPNDTLSKHLFHWDKQPLPWEMRVRVAYHIAQALEYCNAQNRKIYHDLNAYRVLFDEDGDPRLSSFGLMKNSRDGKSYSTNLAYTPPEFLRTGRVIPESVIYSYGTVLLDLLSGKHIPPSHALDLIRGKNVLLLMDSSLEGQYADEDATVMIELASKCLQYEGRDRPDVKFLLTAVAPLQKQKEVASHVLMGLTKTPVAVPTLLSPLGKACARMDLTAVHDILLKTGYRDEEGAENEFGDIAFRDKDFNGAVEYYSKLVSMMSVPSGTIFVRRALSYLMIGQPEPALRDAMQAQVCLPEWPTAFYMQALALSKLGMETDAQDMLNDGSSYEAKRQNSWRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BTD3</accession>
    <name>A0AAD5BTD3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_018523</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011021">
        <property type="protein sequence ID" value="KAI7729303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BTD3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034663">
        <property type="term" value="C:endoplasmic reticulum chaperone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030968">
        <property type="term" value="P:endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.10:FF:000028">
        <property type="entry name" value="Heat shock 70 kDa protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45639:SF34">
        <property type="entry name" value="CHAPERONE PROTEIN DNAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45639">
        <property type="entry name" value="HSC70CB, ISOFORM G-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="104"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="104"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="120"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7729303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="136" mass="14850" checksum="8EEC7FDE840E616B" modified="2024-05-29" version="1">MLRFHAFILKEAEKYKAEDEEHKKKVEAKNALENYAYNMRNTIKDEKIGSKLPEADKKKVEDAVEEAIKWLDGNQLAEAEEFEDKMKELEGVCNPIIAKMYQGGAGGPDMGGGMDDDGPSAGGASGGAGPKIEEVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BV21</accession>
    <name>A0AAD5BV21_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">K Homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_015312</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011114">
        <property type="protein sequence ID" value="KAI7728931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BV21"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009911">
        <property type="term" value="P:positive regulation of flower development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd22459">
        <property type="entry name" value="KH-I_PEPPER_rpt1_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd22460">
        <property type="entry name" value="KH-I_PEPPER_rpt2_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.210:FF:000002">
        <property type="entry name" value="KH domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10288">
        <property type="entry name" value="KH DOMAIN CONTAINING RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="7">
        <location>
            <begin position="41"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="7">
        <location>
            <begin position="140"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="7">
        <location>
            <begin position="274"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="7">
        <location>
            <begin position="361"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="467"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="14"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="467"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7728931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="535" mass="57572" checksum="F76B409C928405C7" modified="2024-05-29" version="1">MAGQRNSYGKRSHSQSDNRDNERSKRRNNSDDRGFNSIGSEDTVYRYLCPSKKIGSIMGRGGEIVKQLRSETKAKIRIGDSVPGCDERVVTIHSTSVETNDFDMNDDQVCPATDALFKVHDRVVTDDQAADTDTDNEGALQVTVRLLVPSDQIGCIIGKGGQVVQSIRSDTDSQIRILKDNNIPACALSNDELVQISGESSNVRKALFQVAARIHDNPSRSQHLLSSSTPNVYPSGGSYMGAPPIMGVTPVVGAYGGYKGEGGFYPGQNESPPREFSLRLVCPTANIGGVIGKGGAAINQIRQETGAAIKVDSSKAEGETDCIISISAKEVFEDTFSPAIEAALRLQPKCSERIERDSGIISYTTRLLVPSSRIGCLIGKGGAIISEIRRISKANIRILSKEQLPKVAEDDDEMVQISAELDLSKDALLQVTSRLRANLFDKEGAMSTFVPVLPYLPVAPDVHDVPKYESYDSRDSRGHGRGHNDLPPNDAYGSYGGLQGHSGGDPYGAYGSYSAGRSGSSGSVMVSSLFRFRIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BW41</accession>
    <name>A0AAD5BW41_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">UDP-glucuronosyl/UDP-glucosyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_013216</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010756">
        <property type="protein sequence ID" value="KAI7730520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BW41"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080043">
        <property type="term" value="F:quercetin 3-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080044">
        <property type="term" value="F:quercetin 7-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926">
        <property type="entry name" value="GLUCOSYL/GLUCURONOSYL TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926:SF774">
        <property type="entry name" value="UDP-GLYCOSYLTRANSFERASE 85A1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7730520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="410" mass="46638" checksum="24C65C6AF8C0010E" modified="2024-05-29" version="1" fragment="single">MNQVVVVPYPGRGHINPLLNLCHSLSSRLNQHNRTTLFTVVVTEEWLGFLNPDQEHDNIRFATIPNVLPSELNRGSNMITFLTAVKTQMEQPFLEVLDNINIPVMLIIADVTMMWPFEVANRRNIPIAAYSPMSASMFSIMHHVDLLESHNHLYVDVSERGRDVIDYIPGLSSLTIEDIPMIFHGGIGEKAKEIIPNLFDITLKANYVMISTMYELEAKAIDALRARIKIPLYICGPNIPIEANPSSNKPMYLEWLDSKQPQTVFYLSVSGIEMVEIAFGLAQSGVNFLWVARGETSHLKEICGDKGIVVEWCDQLRVLSHSSIGGFWTHCGWNSVKESLFSGVPMLTFPIIIDQPFNSKMIVKDWMIGWSVRKKLEVLTRHQIAKIVREFMDPKSVVRIEMMERAKEFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BWY5</accession>
    <name>A0AAD5BWY5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Aladin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_020577</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010871">
        <property type="protein sequence ID" value="KAI7729983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BWY5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006913">
        <property type="term" value="P:nucleocytoplasmic transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045139">
        <property type="entry name" value="Aladin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14494:SF0">
        <property type="entry name" value="ALADIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14494">
        <property type="entry name" value="ALADIN/ADRACALIN/AAAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7729983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="213" mass="23420" checksum="66F0839AAA749174" modified="2024-05-29" version="1">MPSFPQPGSVTLCEINRDLITAQSLSDDEAKDTYGKILGTVFTPIPYQLDHPTPDTAVVEPPSNGLFLKTLLLLLRPNDVKLLNHVDLRCVSWHQKKDILAFVSARNQVTIRDYGDSGEGKDPCILTNDLQREAKLLEWRPNAGKTLSVACKGGICIWTASYPGNTACVRPGVSSGSLSRGSAVRWTLVDFLRSHDNEQISALSWSPDGRYPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BX91</accession>
    <name>A0AAD5BX91_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_032366</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010491">
        <property type="protein sequence ID" value="KAI7731252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BX91"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7731252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="47" mass="5309" checksum="672F78B51E4D313B" modified="2024-05-29" version="1">MCVQFSILYPPTCIIIHIIVPSISSALTSHKQVDAQKLRLKCRLNQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BXF6</accession>
    <name>A0AAD5BXF6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_030266</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010516">
        <property type="protein sequence ID" value="KAI7731195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BXF6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016504">
        <property type="term" value="F:peptidase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070628">
        <property type="term" value="F:proteasome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010499">
        <property type="term" value="P:proteasomal ubiquitin-independent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035309">
        <property type="entry name" value="PSME4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32170">
        <property type="entry name" value="PROTEASOME ACTIVATOR COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32170:SF3">
        <property type="entry name" value="PROTEASOME ACTIVATOR COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7731195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="183" mass="20984" checksum="037C4C30A3B65909" modified="2024-05-29" version="1" fragment="single">MDYRYLLEKKSDDSILLLLLIRIMEALGNYGSAEYEEWNNHRRAWKLESVAIVEPPVNFIVPSNSKGKKRPRWAVIDKACMHNTWRTSQSSYNLYRTSKNASPSENLDILMNDLLNLCVHSYDTVRTLAVRSLVKLIKRWPCLIAKCVLTLTENLRSPSSPEYMVLGSCAILGTQTVLKHLTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BYL3</accession>
    <name>A0AAD5BYL3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Single-stranded DNA binding protein Ssb-like OB fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_027497</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010389">
        <property type="protein sequence ID" value="KAI7731765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BYL3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd04491">
        <property type="entry name" value="SoSSB_OBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048970">
        <property type="entry name" value="OB_Ssb-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31472:SF36">
        <property type="entry name" value="NUCLEIC ACID-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31472">
        <property type="entry name" value="OS05G0244600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21473">
        <property type="entry name" value="OB_Ssb-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Single-stranded DNA binding protein Ssb-like OB fold" evidence="2">
        <location>
            <begin position="34"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF21473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7731765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="157" mass="17017" checksum="D6A36CE93650D6C7" modified="2024-05-29" version="1">MSAVTNTTSSEPENQQTARGVVKRKPVFVKVGDLKPGTNGHTLAVKVLSSTTVLDKKVRNTSSFSGRPGGSNTRIAECLVGDETGTILFTARNDQVDLMKAGSTVIIRNAKIDMFKGSMRLAVDKWGRIEVTDPATFTVKEDNNLSLVEYELVNVTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C016</accession>
    <name>A0AAD5C016_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Dof zinc finger protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_024193</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Transcription factor that binds specifically to a 5'-AA[AG]G-3' consensus core sequence.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010205">
        <property type="protein sequence ID" value="KAI7732587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C016"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045174">
        <property type="entry name" value="Dof"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003851">
        <property type="entry name" value="Znf_Dof"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31992:SF355">
        <property type="entry name" value="DOF ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31992">
        <property type="entry name" value="DOF ZINC FINGER PROTEIN DOF1.4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02701">
        <property type="entry name" value="Zn_ribbon_Dof"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01361">
        <property type="entry name" value="ZF_DOF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50884">
        <property type="entry name" value="ZF_DOF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 8 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3 9" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 8 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Dof-type" evidence="11">
        <location>
            <begin position="64"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="16"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="108"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="17"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="114"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="129"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU369094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7732587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="306" mass="33750" checksum="A9E6F7C748FD8AB7" modified="2024-05-29" version="1">MVFPSVAPFLEHHNWHQQLQQQPNPQQENDGGGQNPNFQTGSIRPGSMVDRARLAKLPMPEPGLNCPRCDSTNTKFCYFNNYSLTQPRHFCKACRRYWTRGGALRNVPVGGGCRRNKRSNKNSRRSKSPSQKGTKSLNGSPSRCSNENMASTQLTHLPPLQMPFMSSYGGVASNMTSSTLGGFHPQSEIGNFHLGIGGSNGTNLNNILSIGSGESWRLPFLAGFEVPNNTNLFLYQSEGVVDAPASSMLENTQINPPVKVEENRDQNLPRQIMNISENTNQQPWQVGNNWADQFSGVSASSTPKQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C092</accession>
    <name>A0AAD5C092_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">E3 ubiquitin-protein ligase RMA</fullName>
            <ecNumber evidence="11">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Protein RING membrane-anchor</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">RING-type E3 ubiquitin transferase RMA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_024146</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">E3 ubiquitin-protein ligase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 11">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Endoplasmic reticulum membrane</location>
            <topology evidence="11">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">The RING-type zinc finger domain is responsible for E3 ligase activity.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="11"/>
    <dbReference type="EMBL" id="JAMZMK010010205">
        <property type="protein sequence ID" value="KAI7732540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C092"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16745">
        <property type="entry name" value="RING-HC_AtRMA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045103">
        <property type="entry name" value="RNF5/RNF185-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12313">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13923">
        <property type="entry name" value="zf-C3HC4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 11" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 10 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="13">
        <location>
            <begin position="114"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="302"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="312"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU369090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7732540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="336" mass="37700" checksum="2ECC55926A9C9B56" modified="2024-05-29" version="1">MGDETPQNVMNLDLNLGPVETETEPRGLNLDNWLDGPVGDPVPVGPRTVVRRRRWRSVWRPVSGEGSVLADDGGLPPEMTKTCDNGIAQQANGLVDEKGHMVKSNESEGGFFDCNICLDMASDPVVTYCGHLFCWPCLYRWLHVHSDVKECPICKGEMTMKTVTPIYGRGNPTRVVEEDSSLKIPNRPQAKRVESFRQTIQRNAFTMPMEEMIRRLGSRFDLTRDLFQGHSFLSENSSLLNRYLASRRLRTDQDQNPTLVEPFHSNQEQAPSMDDRDSVSSIAAVIQSESQTVDTAVEIDSRVSLSTSSSRRRSESSRVSDVDSGDSRAARRRRLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C0B5</accession>
    <name>A0AAD5C0B5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">M8C21_033425</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">AA19_3_7</strain>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">Interacts with ARAC5 and ARAC10.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010010094">
        <property type="protein sequence ID" value="KAI7733068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C0B5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051020">
        <property type="term" value="F:GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000335">
        <property type="entry name" value="receptor-like cytosolic serine/threonine-protein kinase RBK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046958">
        <property type="entry name" value="RBK1/2/STUNTED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47987">
        <property type="entry name" value="OS08G0249100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47987:SF7">
        <property type="entry name" value="PROTEIN KINASE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="244"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="19"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="55"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="273"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00063228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAI7733068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="556" mass="62586" checksum="4D8B8CFC26AC515A" modified="2024-05-29" version="1">MFLTLDTEQSQETHKIIQIEDEEEDDDDDHKQHDESSPRGVFEILLSGSDSDNHSTVSRSLSSRGSSFNEKSPELEPDGDCATECGGNGLISSSSMAQDIRWRHLVCNLKWKSFKRFSTVPLVAGYEMSRKSLMRRLGRHNVGKEMVDGVRWMVRKPSWRNFTLDELSVATGGFCATWRRLSAGKGLKATWRRLSAGKGLRHFPSISSLYLSSLCCAERSPPKGFLPLSTCDSIILEGSLETIETLENLVGRGGHAEVYKGCLPDGQLVAVKKITKKEKKDEDRVEDFLSELGIIAHINHPNAAHLIGFSSDTDLHLVLQFAPHGSLATLLHNNEERLDWALRFKIAIGIAEGLLYLHSQCQRRIIHRDITASNILLMEDYQPQISDFGLAKWLPDKWLHHIVSPIEGTFGYMAPEYFMHGIVHEKIDVFSFGVLLLELITGRRAVDSSRQSLVIWAKPFLEQSNVKELVDPRLEGKYEMIEVKRAMSAASACIHHLPSVRPNMKRVMQMLKGERSEPKPKIGGGRALLIDACDLDDYSSTTYLKDLNRHRELVMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C110</accession>
    <name>A0AAD5C110_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Longin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_003582</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in the targeting and/or fusion of transport vesicles to their target membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Endomembrane system</location>
            <topology evidence="8">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the synaptobrevin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010004">
        <property type="protein sequence ID" value="KAI7733428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C110"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14824">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.50:FF:000014">
        <property type="entry name" value="vesicle-associated membrane protein 727"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.50">
        <property type="entry name" value="Longin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010908">
        <property type="entry name" value="Longin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051097">
        <property type="entry name" value="Synaptobrevin-like_transport"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21136">
        <property type="entry name" value="SNARE PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21136:SF169">
        <property type="entry name" value="VESICLE-ASSOCIATED MEMBRANE PROTEIN 727"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13774">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01270">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50859">
        <property type="entry name" value="LONGIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Longin" evidence="9">
        <location>
            <begin position="1"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7733428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="167" mass="18945" checksum="9D93A55BC155C402" modified="2024-05-29" version="1" fragment="single">MNRKGLIYSFVAKGTVVLAEHTAYSGNVSTIAVQCLQKLPSNSSRYTYSCDGYTFNFLLDLGFVFLVVADESMGRIVPFVFLERLKDEFKQVYGGSIGSDNSLAEDSDDDLFEDRFSIAYNLDREFGPRIKEQMDYCLNHPDEMNKLSKLKAQITEVKGIMMDNIEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C1W3</accession>
    <name>A0AAD5C1W3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Purple acid phosphatase</fullName>
            <ecNumber evidence="9">3.1.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_012616</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a phosphate monoester + H2O = an alcohol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15017"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:67140"/>
            <dbReference type="EC" id="3.1.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4 9">Belongs to the metallophosphoesterase superfamily. Purple acid phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.2" evidence="9"/>
    <dbReference type="EMBL" id="JAMZMK010010209">
        <property type="protein sequence ID" value="KAI7732516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C1W3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003993">
        <property type="term" value="F:acid phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00839">
        <property type="entry name" value="MPP_PAPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.380">
        <property type="entry name" value="Purple acid phosphatase-like, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041792">
        <property type="entry name" value="MPP_PAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039331">
        <property type="entry name" value="PAPs-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025733">
        <property type="entry name" value="PAPs_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015914">
        <property type="entry name" value="PAPs_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008963">
        <property type="entry name" value="Purple_acid_Pase-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22953">
        <property type="entry name" value="ACID PHOSPHATASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22953:SF158">
        <property type="entry name" value="PURPLE ACID PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14008">
        <property type="entry name" value="Metallophos_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16656">
        <property type="entry name" value="Pur_ac_phosph_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49363">
        <property type="entry name" value="Purple acid phosphatase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041768727" description="Purple acid phosphatase" evidence="9">
        <location>
            <begin position="20"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="domain" description="Purple acid phosphatase N-terminal" evidence="12">
        <location>
            <begin position="44"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="10">
        <location>
            <begin position="140"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="Purple acid phosphatase C-terminal" evidence="11">
        <location>
            <begin position="347"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF14008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF16656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7732516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="422" mass="47479" checksum="61C95E382C280982" modified="2024-05-29" version="1" fragment="single">KSPYHVLSLLIITTGLAAAGDYVRPAPGKSLYVSLAGDHSKNAPQQVHISLAGDAKMRISWITDDMTPSMVKYGKSPGNYEGSATGTSSTYRYILYKSGHIHDVVIGPLEPSTTYYYKCGLDSSPELSFRTPPVQFPIDFAVSGDLGQTEWTKSTLQHIGETGYDVLLLPGDLSYADFYQPSWDSFGRLVEPLASQRPWMVSQGNHEVERIPFIHKERFTAYNARWHMPFEESGSNSNLFYSFEVAGVHVIMLGSYTDFGPGSDQYKWLESDLKKVDRNRTPWLFALVHAPWYNSNTAHQGEKESTHMRDAMEDLLYNARVDIVFAGHVHAYERFTRVYKQQFDKCGPVHITIGDGGNREGLATRYKDPQPKISLFREASFGHGQLRVVNASHAKWTWHRNDDAQPDSMWFTSLASDSACVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C3C1</accession>
    <name>A0AAD5C3C1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_025408</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009686">
        <property type="protein sequence ID" value="KAI7734613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C3C1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042138">
        <property type="term" value="P:meiotic DNA double-strand break formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044969">
        <property type="entry name" value="DFO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37176">
        <property type="entry name" value="F10K1.23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37176:SF1">
        <property type="entry name" value="PROTEIN DOUBLE-STRAND BREAK FORMATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7734613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="167" mass="19237" checksum="E49ABC5424541D55" modified="2024-05-29" version="1" fragment="single">MRHESVCVLREINGTPVDQKLLIVEFFSCLALRYEALVLREMNYAVDQLLRVEYSEWFAFAKQAVDNRFYAIARKACEKALACLQTNDILKTKDADALCEHMETIRKVKRLKDVATVRGSSQSVQAQTVEYLKRKITEHDEASTLVIKEKQHSASFLFRNGIKKHNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C3L8</accession>
    <name>A0AAD5C3L8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Flavin-containing monooxygenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_004363</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FMO family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009706">
        <property type="protein sequence ID" value="KAI7734507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C3L8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004499">
        <property type="term" value="F:N,N-dimethylaniline monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000099">
        <property type="entry name" value="Flavin-containing monooxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000960">
        <property type="entry name" value="Flavin_mOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020946">
        <property type="entry name" value="Flavin_mOase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050346">
        <property type="entry name" value="FMO-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23023">
        <property type="entry name" value="DIMETHYLANILINE MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00743">
        <property type="entry name" value="FMO-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000332">
        <property type="entry name" value="FMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00370">
        <property type="entry name" value="FMOXYGENASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7734507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="355" mass="40253" checksum="791DCB33A780A449" modified="2024-05-29" version="1" fragment="single">MSRQSITHQFLYIHKQIHNPKHITIPIILSTMSPITTCNVAVIGAGAAGLAAGRHLRREGHSVTIFEKESQVGGIWVYTNTTEPDPLGLDPTRPVIHSSLYSSLRTNLPRETMGFREYPFVVRKSGQRDPRRFPGHEEVLEYLKEYANEFGIGELVRFGTEVSRVWRRECGKWEVRFKRCGDEYEEDFDAVVVCNGHYTEPRIAYMAGVDRWPGKQIHSHNYRVPEPFQNKIVVLIGSSASAVDISREIASVANEVHIASWSNTDEESATLPGYDNIWLRPMIESANEDGTISFPHGFKISADIIMHCTGYKYHFPFLDTQGIVTVDDNRVGPLYKHVFPPSLAPWLSFVGLPWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C5W0</accession>
    <name>A0AAD5C5W0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_000559</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009435">
        <property type="protein sequence ID" value="KAI7735797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C5W0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7735797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="120" mass="13501" checksum="267A8B89589EFF3E" modified="2024-05-29" version="1" fragment="single">ILSPTFHLQPPLKLSQPLLAIPHSTSRLEISQSREPPMAMMTEGVTASPVLCHSRQRCSEYGLVRLSDILIPVEYDVTEVFNVHRFTCSLTLSVAKLAVYIALNQDFEAELRSTDDEFQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C976</accession>
    <name>A0AAD5C976_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Gnk2-homologous domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">M8C21_003960</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">AA19_3_7</strain>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cell junction</location>
            <location evidence="12">Plasmodesma</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the cysteine-rich repeat secretory protein family. Plasmodesmata-located proteins (PDLD) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008974">
        <property type="protein sequence ID" value="KAI7737656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C976"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009506">
        <property type="term" value="C:plasmodesma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010497">
        <property type="term" value="P:plasmodesmata-mediated intercellular transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046739">
        <property type="term" value="P:transport of virus in multicellular host"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23509">
        <property type="entry name" value="Gnk2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.430.20:FF:000001">
        <property type="entry name" value="cysteine-rich repeat secretory protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.430.20">
        <property type="entry name" value="Gnk2 domain, C-X8-C-X2-C motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051378">
        <property type="entry name" value="Cell2Cell_Antifungal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002902">
        <property type="entry name" value="GNK2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038408">
        <property type="entry name" value="GNK2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32080">
        <property type="entry name" value="ANTIFUNGAL PROTEIN GINKBILOBIN-2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32080:SF53">
        <property type="entry name" value="GNK2-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01657">
        <property type="entry name" value="Stress-antifung"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51473">
        <property type="entry name" value="GNK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0965">Cell junction</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0945">Host-virus interaction</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Gnk2-homologous" evidence="15">
        <location>
            <begin position="9"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="225"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="241"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAI7737656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="273" mass="30102" checksum="99C02B8B9E1C527B" modified="2024-05-29" version="1" fragment="single">VISAAPDNSKLVYKGCANQPLPDPNGVYSSSLSAIFGTLIQQSSKSKFYKTTSGSVSGLFQCRGDLSNVDCYTCVSRLPILMDKLCGKTVAARIQVLGCYMLYEVSGFAQISGMELLYKTCGKTNGGFQQRRDAAFSALESVVVYPKVKVIIQDEEEEEYNPPRVYLKHPSSYDHPSNSPEHKDGYTYTPPYVRLPISTSPMAMGKANRESNSKSASVLPPRAVLSSPENDAIHGAKIKKTQGSGLKNHNLSQNRHVKCTKSYELDNRRRSWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CC19</accession>
    <name>A0AAD5CC19_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">VIN3-like protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_018584</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008775">
        <property type="protein sequence ID" value="KAI7738623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CC19"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040029">
        <property type="term" value="P:epigenetic regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010048">
        <property type="term" value="P:vernalization response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15521">
        <property type="entry name" value="PHD_VIN3_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058585">
        <property type="entry name" value="Fn3_VIN3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032881">
        <property type="entry name" value="Oberon-like_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044514">
        <property type="entry name" value="VIN3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056990">
        <property type="entry name" value="VIN3-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46286:SF2">
        <property type="entry name" value="VIN3-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46286">
        <property type="entry name" value="VIN3-LIKE PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23376">
        <property type="entry name" value="Fn3_VIN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07227">
        <property type="entry name" value="PHD_Oberon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23380">
        <property type="entry name" value="VIN3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Oberon-like PHD finger" evidence="7">
        <location>
            <begin position="135"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="VIN3-like fibronectin type-III" evidence="8">
        <location>
            <begin position="338"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="VIN3-like C-terminal" evidence="9">
        <location>
            <begin position="533"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="91"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="423"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="436"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="498"/>
            <end position="507"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF23376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7738623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="618" mass="69836" checksum="32A9BC5CCCF3571D" modified="2024-05-29" version="1">MDSCSFEGFVLDPSKCSKLSMEEKREIVYEVSKWSHGAPELLQSWSRQEILQILCAEMGKERKYTGLTKIKIIEHLLKIVSEKRSEDDITVNLESSESDHQRSAKRHRKSEQPNRLNVEQDHNYVDLEDSNVKFCKNSACRAKLSQDGAFCKRCSCCICHQYDDNKDPSLWLMCSSDPPFHGESCGMTCHLECALKHDKSGIPKDGRIRGLDGSFHCVSCGKVNDLLGCWRKQLTIARDTRRVDILCYRVSLSQKLLVGTLKYQKLHEIVNEAMEKLEADVGQLTGLPVKRARGIVNRLSSGQEIQKLCAFAVESVDSVLFNMTLSDAADPSILVPIVKFGNIFPTSISLILGARDQPVTDNAKTQRYIMWHRKAEETDYPAKPTCTLFAPSCTKFLLSGLTPSTQYVLKVVHFENTRELGSKEMSFQTTNDDVKSPPTNSSSLSNPSSVEDENNTIVNNKQKDKEVAKTSINDENNEKDFDPFAPTTSAKLPITPFKTERVKDSILPRKTRSKSSKKNLDNESEDEPNESNDRDFGYYVKVIRWLECEGHVDTGFRQKFLTWYSLRASQQEVRIVKVFVDTLMEDPASLAGQLVDTFSDVITNKTCSSKGLCLKLFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CCA1</accession>
    <name>A0AAD5CCA1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">TFIIF beta subunit HTH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_021091</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TFIIF beta subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008720">
        <property type="protein sequence ID" value="KAI7738794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CCA1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005674">
        <property type="term" value="C:transcription factor TFIIF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000035">
        <property type="entry name" value="General transcription factor IIF subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003196">
        <property type="entry name" value="TFIIF_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040450">
        <property type="entry name" value="TFIIF_beta_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011039">
        <property type="entry name" value="TFIIF_interaction"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10445">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIF SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10445:SF2">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIF, BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02270">
        <property type="entry name" value="TFIIF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50916">
        <property type="entry name" value="Rap30/74 interaction domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TFIIF beta subunit HTH" evidence="8">
        <location>
            <begin position="226"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="55"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="206"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="275"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="55"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="65"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="208"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="284"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7738794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="300" mass="34128" checksum="BF849C22E3191FE3" modified="2024-05-29" version="1">MDDDQITTTVIKPSGTAAKPNNVVVQPSLETMKSERHVWLMKCPPIVSRSLMQKQSHLTTPTLDSNSNSNTNSSSSAPVGAKVIVTVDPLLPNNDFSSTQFTMELLGTELGRVPKCYSMDMSTDFIPMSVFSESKQASGRVSVEGKIYHKFDMKPHNENIEEYGKLCRERTNKYMTKTRQIQVIDNDKGAHMRPMPGLIPLRLLSPTADREKRKTSVKGSDTKRTRRDRGEMEDIMFKLFERQSNWTLKHLISETDQPEQFLKDLLKDLCIYNNKGSNQGTYELKPEYKRSTEEQEPASK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CDZ2</accession>
    <name>A0AAD5CDZ2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_008617</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Functions as an E3 ubiquitin ligase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="JAMZMK010008740">
        <property type="protein sequence ID" value="KAI7738726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CDZ2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16655">
        <property type="entry name" value="RING-Ubox_WDSUB1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051348">
        <property type="entry name" value="U-box_ubiquitin_ligases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003613">
        <property type="entry name" value="Ubox_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45647">
        <property type="entry name" value="OS02G0152300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45647:SF15">
        <property type="entry name" value="U-BOX DOMAIN-CONTAINING PROTEIN 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04564">
        <property type="entry name" value="U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00504">
        <property type="entry name" value="Ubox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51698">
        <property type="entry name" value="U_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="290"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="domain" description="U-box" evidence="10">
        <location>
            <begin position="551"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="78"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="258"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="88"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="100"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7738726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="621" mass="69324" checksum="37D2B3B1353E2097" modified="2024-05-29" version="1" fragment="single">VAVEIVQIESNDVVDAIKQAVVKNKINKLVIGASSTSIFSRGQQNLSSRISESIPSFCTVYVISKGILSSLRAADSETIGSSKDDSNSSDSSFVTNSSNVAPSSRTEWTDQGSATSYSQSFSNSLPMQRFEALSSINRTLLKKTLIESSENKFIKSCEGDDATVSDASNHDEFLENSIVSSSRSFLTESQSWTSDEDSIPGAPSEIASDIHDLKNSPLQDNSINFELEKLKVKLRHIRGMYAIAQSESFDASRKLTDIKKLQLEESAKLKDLEAKEQEAKSLAEEERKQHKAAKRLAEYAKECIRREATLRKDVEEMALQDSRDKQKLQHAITGTSLRYREFTWEEIVDACSSFSSYGCCCQSSSFSRGSWNQTISTRVLSRIRHPHLLILIGACVDHGCLVYEYMENGSLDERLFRKNDTSPIPWFDRFRIAWEVASALNFLHNAKPKSIVHRDLKPANILLNKNLVSKIGDVGLSTMLQSDFASTSTIYKDTSEWPIDEAKELAILGLSCAELRRKDRPDLKNGVLPILERLKAVADVAQRSTSISRSTPPNHFICPIRKDVMVDPCVAVDGYTYDRNAIEQWFEESDSSPMTNLPLTSRSLTPNYALLSAILEWKSTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CED7</accession>
    <name>A0AAD5CED7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_024435</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008417">
        <property type="protein sequence ID" value="KAI7740523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CED7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016671">
        <property type="term" value="F:oxidoreductase activity, acting on a sulfur group of donors, disulfide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010190">
        <property type="term" value="P:cytochrome b6f complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044241">
        <property type="entry name" value="TxlA/HCF164"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47353">
        <property type="entry name" value="THIOREDOXIN-LIKE PROTEIN HCF164, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47353:SF1">
        <property type="entry name" value="THIOREDOXIN-LIKE PROTEIN HCF164, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7740523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="177" mass="19193" checksum="ECB4D44433B21A5F" modified="2024-05-29" version="1">MARVASNPKNLIVESGSDKDNKKGEDTSSPSGGGFPELPNKSLNRRIAVVSVLGAIGLFSSGRLDFGVSLKDLSAAALPYEENLFFFDHPSTSVNNVAFSSFFFGHRKPASTTLPEKEVTGEKCEEGNIVNAGFRWLVTRICFSFPVVVDNGVLVWRMWWWLTGFSDGKYFSGGGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CFN7</accession>
    <name>A0AAD5CFN7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_013887</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008385">
        <property type="protein sequence ID" value="KAI7740639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CFN7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042054">
        <property type="term" value="F:histone methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.280.10">
        <property type="entry name" value="SRA-YDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025794">
        <property type="entry name" value="H3-K9-MeTrfase_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051357">
        <property type="entry name" value="H3K9_HMTase_SUVAR3-9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007728">
        <property type="entry name" value="Pre-SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036987">
        <property type="entry name" value="SRA-YDG_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003105">
        <property type="entry name" value="SRA_YDG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45660:SF74">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE CHROMATIN REMODELING SET FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45660">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SETMAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05033">
        <property type="entry name" value="Pre-SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02182">
        <property type="entry name" value="SAD_SRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00468">
        <property type="entry name" value="PreSET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00466">
        <property type="entry name" value="SRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50867">
        <property type="entry name" value="PRE_SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51575">
        <property type="entry name" value="SAM_MT43_SUVAR39_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51015">
        <property type="entry name" value="YDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="2" id="KW-0158">Chromosome</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YDG" evidence="9">
        <location>
            <begin position="252"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="Pre-SET" evidence="8">
        <location>
            <begin position="478"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="domain" description="SET" evidence="7">
        <location>
            <begin position="539"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="80"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="172"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="180"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7740639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="695" mass="77196" checksum="C5737FB300C43B9E" modified="2024-05-29" version="1">MCSVIDLNHHPDVTFTGDYVTTAGTQNTPKIEPKEEPFDDAITQPHASSQLAVHAGGDDEPDVYSEFNRVSEMFRAAFSGKTGPNRVRSNPKPAGDPDSLAIVSVPEENRHLAVSDPGLQRKQPDPGRRRKQLRSSELVRVTNLGVEDERCFRGVVRKTRMIYDSLRVFVSTEDEKRRNSNSNSNGNNNSNSTSDSNSNDINGNININSNSNSNSNSNNLSGRNPHARGHLKSASVMKDRGLWLHRDKRIVGAIPGVYVGDIFFFRMEICVVGMHGLPQAGIDYLSSSQSSNGEPIATSIIVSGGYEDDQDAGDVIIYTGHGGQDKFSRQADHQRLEGGNLAMERSMCYGLEVRVVRGFRYEGSPSGKVYVYDGLYKVVDSWLEAGKSGFGVIKFKLVRMENQPEMGSAILKYAASLRKGPLQVMPGGYVSMDMSMKKEHVPVFLFNDIDDNHEPMFYEYLVTSVFPPFVYHHGAVGGGCDCVSGCIDGCFCAKRNGGDFAYDQNGLLVRGKPLIFECGPHCSCPPNCRNRVSQNGVKNKFEVFRSHETGWGVRSLDLIQAGSFICEYTGVILTRQQAQLFSMNGDNLIYPNRFGERWAEWGDLSQVFSDYERPSYPSVPPLDFAMDVSRMRNVACYLSHSLCPNVLVQLVLYDHSNYGFPHLMLFAMENIPPMRELSLDYGEADEWTGKLSICN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CFU1</accession>
    <name>A0AAD5CFU1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">M8C21_023525</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">AA19_3_7</strain>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010008361">
        <property type="protein sequence ID" value="KAI7740714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CFU1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000430">
        <property type="entry name" value="Protein kinase superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000466">
        <property type="entry name" value="Putative LRR receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047117">
        <property type="entry name" value="PERK1-13-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47982:SF20">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47982">
        <property type="entry name" value="PROLINE-RICH RECEPTOR-LIKE PROTEIN KINASE PERK4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 13 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="78"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAI7740714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="379" mass="41833" checksum="331C5D3FE1A156E4" modified="2024-05-29" version="1">MSRTLAAIVGGAAGAMALLGFVILVTWFFFCNNRSASRTSETDSSDPSQVERSQAVQLVARETRRFEIDELSLATKNFSDKSLIGEGKFGEVYKGLLHDGMLVAIKRRLAPPTQEFIQEARYLASIQHRNIVTLLGYCQASGQQILVYEYVPNGSVSIHLYGAGHVAKEKLEFKHRLSIALGAAKGLAHLHSLSPRLAHKDFKTANVLVDENFIAKVADAGVRNFLGRVDAAGPSSQTRIDEIFLAPEVREFRRFSEKSDIYSFGVFLLELVSGQEAAELLSSNTNHNLVEWVHNHQEARTISSIIDPRIGNSFTTEGMEEYIDLMTGCVEVSSERRPAMSHVVSELDRILEKEMSLTTIMGEGTPVVTLGSQLFRAAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CGT9</accession>
    <name>A0AAD5CGT9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TauD/TfdA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_029257</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008208">
        <property type="protein sequence ID" value="KAI7741352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CGT9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.130.10">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050411">
        <property type="entry name" value="AlphaKG_dependent_hydroxylases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042098">
        <property type="entry name" value="TauD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003819">
        <property type="entry name" value="TauD/TfdA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10696">
        <property type="entry name" value="GAMMA-BUTYROBETAINE HYDROXYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10696:SF43">
        <property type="entry name" value="TAUD_TFDA-LIKE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02668">
        <property type="entry name" value="TauD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TauD/TfdA-like" evidence="2">
        <location>
            <begin position="43"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7741352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="323" mass="36037" checksum="C300BA7D045F0896" modified="2024-05-29" version="1">MATGRLFQEVELPEQRSYDDGLVFPVVLSPTTKTNIIQCIRSEKLWLESLLQKRGVILFRGFPVTSPSDFNDVVEAFDFPELLYVGGRAPRTKVVGRVYTANDSPLDKIIPFHHEMASMPDFPSKLFFFCAEEPKSGGETPILLSHIVYEKMKAKHPDFVAQLEEHGLTNTKIMSDEDRPSIPSGRGWKSTFMTDDKNVAEERAAKLGTKLEWIGNAAKVITGPLPAIRLDKGSQRKTWFNNLVGSNTAPIRDDIDDYDAHVELGNGDLLPDHAVKDCMKIMEEECVAIPWKKGDVMLGGEARNKAGMDGECCETYNRSSAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CH54</accession>
    <name>A0AAD5CH54_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Subtilisin-like protease SBT5.3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_000640</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the peptidase S8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008134">
        <property type="protein sequence ID" value="KAI7741796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CH54"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02120">
        <property type="entry name" value="PA_subtilisin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04852">
        <property type="entry name" value="Peptidases_S8_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.2310:FF:000002">
        <property type="entry name" value="p69E protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000006">
        <property type="entry name" value="Subtilisin-like protease SBT1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.30:FF:000005">
        <property type="entry name" value="subtilisin-like protease SBT1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.80:FF:000002">
        <property type="entry name" value="Subtilisin-like protease SBT5.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.80">
        <property type="entry name" value="Peptidase S8 propeptide/proteinase inhibitor I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034197">
        <property type="entry name" value="Peptidases_S8_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010259">
        <property type="entry name" value="S8pro/Inhibitor_I9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037045">
        <property type="entry name" value="S8pro/Inhibitor_I9_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045051">
        <property type="entry name" value="SBT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041469">
        <property type="entry name" value="Subtilisin-like_FN3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10795">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17766">
        <property type="entry name" value="fn3_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05922">
        <property type="entry name" value="Inhibitor_I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 8" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0720">Serine protease</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Inhibitor I9" evidence="11">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="9">
        <location>
            <begin position="115"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="domain" description="PA" evidence="10">
        <location>
            <begin position="372"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="domain" description="Subtilisin-like protease fibronectin type-III" evidence="12">
        <location>
            <begin position="645"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7 8">
        <location>
            <position position="124"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7 8">
        <location>
            <position position="194"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7 8">
        <location>
            <position position="532"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF05922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF17766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7741796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="745" mass="80102" checksum="0DAC155474671688" modified="2024-05-29" version="1" fragment="single">SYVVYLGAHSHNNLEASLIDLDQVKDRHYEFLGSCLGSKDKAKDAIFYSYTRHINGFAAILEDEEAAHIAKHPRVVSVFLNRGRKLHTTRSWDFMGLEDNGIISSSSIWKKARFGENTIIGNLDTGVWPESKSFSDEGMGAVPSKWKGVCQNGVDASFRCNRKLIGARYFNKGYASMVGPLNSTFESPRDHEGHGSHTLSTAGGNFVSDASVFGYGNGTAKGGSPNARVAAYKVCFPPVNGNECFDADILAAFDMAIHDGVDVLSVSLGGDAVPFFNDSVAIGSFHAVKHGIVVVCSAGNSGPIDATVSNVATWQITVGASTMDRQFPSYAVLGNNLRFKGESLSVKALPKNKLFPVISSVEAKAANADAIDAQLCKAGSLDPKKAKGKILVCLRGDNARVDKGQEAALAGAVGMILSNNEASGNEIIADPHVLPATHITYADGLAVYRYLNSTKTPVAYITHPSTQLDTKPAPFMAAFSSKGPNTITPEILKPDITAPGVSIIAAYTESQGPTNEDFDTRRVQYNCVSGTSMSCPHVSGIVGLLKTLHPDWSPAAIRSAIMTTARTRDNEAKPMTNASHIKATPFSYGAGHIQPNRAMDPGLVYDLDTTDHLNLLCALGYTKSQIEKFSETPYTCPSKNISLLDFNYPSITVPNLNESVTVTRTVKNVGSPAATYTSQVFKPAGVSVQVQPKSLKFKKIGEEKMFKVILKSKMSRRAVGEYVFGQLKWSDGKHNVRSPIVVKTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CHY1</accession>
    <name>A0AAD5CHY1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">tRNA/rRNA methyltransferase SpoU type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_026913</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008073">
        <property type="protein sequence ID" value="KAI7742118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CHY1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002130">
        <property type="term" value="P:wobble position ribose methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18094">
        <property type="entry name" value="SpoU-like_TrmL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1280.10:FF:000002">
        <property type="entry name" value="Peptidylprolyl isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01885">
        <property type="entry name" value="tRNA_methyltr_TrmL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001537">
        <property type="entry name" value="SpoU_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016914">
        <property type="entry name" value="TrmL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42971">
        <property type="entry name" value="TRNA (CYTIDINE(34)-2'-O)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42971:SF1">
        <property type="entry name" value="TRNA (CYTIDINE(34)-2'-O)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00588">
        <property type="entry name" value="SpoU_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="alpha/beta knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA/rRNA methyltransferase SpoU type" evidence="6">
        <location>
            <begin position="78"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7742118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="259" mass="28457" checksum="15E2FAADF9F13DBB" modified="2024-05-29" version="1">MLTTQCLKTLDFLTTPIHFRRSVTAPLRYPTSLLLQPKNKFNSPLCSLSENSSSSSNKEGLVNGISVAGSKVSEAKLLQVVLVSPQIPGNTGCIARSCAASGVGLHLVEPLGFEVDDTKLKRAGLDYWPYVVVKVHSSWANFRDYFKQQSGEKRLLAFTKRGTNIHSDFSYKRGDWLVFGSETTGLPPEALDDCKNEPLGGGTLKIPMVETYVRCLNLSVSVGIGLYEAARQLNYEQLQADSEPCNDTRQLSFAEDVFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CI86</accession>
    <name>A0AAD5CI86_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glucose/ribitol dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_012003</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008019">
        <property type="protein sequence ID" value="KAI7742313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CI86"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05327">
        <property type="entry name" value="retinol-DH_like_SDR_c_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055280">
        <property type="entry name" value="TIC32"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48476">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE TIC 32, CHLOROPLASTIC-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48476:SF3">
        <property type="entry name" value="VERY-LONG-CHAIN 3-OXOACYL-COA REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7742313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="315" mass="34096" checksum="EC9C27F28AEEB2EB" modified="2024-05-29" version="1">MTWISIFTGKSGFAASSTAEDVTKGIDGTGVTAIVTGATSGIGLETARVLALRGVNVVMPVRNIESGEKVKQSIAEKIPNAKLEVMELDVSSLESVRKFASQYCSKGLPLNILILNAGIMTPPFTLSKDNIELQFATNHVGNFLLTNLLLDTMKKTAKESGKEGRIVILSSEVHRVTYKGGIMFDKLNDEKSYNALYAYGQSKLANALHAYELTRRLQEEGVNITANSLHPGVIKTNLARHGGFFSVFVNAVQSFLKNVPQGAATTCYVALHPQLKGVSGQYFADSNLSKASGYARDPELAKKLWDFSVKLTESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CIE5</accession>
    <name>A0AAD5CIE5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_001628</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008065">
        <property type="protein sequence ID" value="KAI7742169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CIE5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="22"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7742169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="95" mass="10771" checksum="C5FB83726B96BD53" modified="2024-05-29" version="1">MASRCNRLMNKASISSIKSAFMSAPKSPSSSPKFPLPSRSTASPVPRFSVSRHMIFQCMREMNWDLQDLSSYHSWVYPFQGDLLAESLDQDTLPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CIY4</accession>
    <name>A0AAD5CIY4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_007623</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007977">
        <property type="protein sequence ID" value="KAI7742462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CIY4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37381">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT (PPR) SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37381:SF1">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT (PPR) SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="190"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="578"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7742462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="578" mass="63298" checksum="BA032B8998B83C20" modified="2024-05-29" version="1" fragment="single">PYKLPTSRSSSSPPLFVLTSISHTHTYRMTLNLTSPWPNRLHLHSTHHHRFLSTTTEPTYMFCSFRTPNYKTNNNNIKVSLSESYHERAVVDGDDDAAASSSSSSSEYDLIISKVSTAKDATEALQVFGEMSKRSGGVVTTSDCCSIISAALNRNNAELALSVFQAMRSSFDAGVGDKDVGDRWRWSRPDVNTYTLLVRGLAASLRVSDALKIIANVCRVGVSPGEEVPFGKIVKCPSCMIAVAVAQPQNGCPPAERSMDVPAWKRGLQFLQLIKETTPAAVHSIVQTPSGMARTHRFATKTLDLPAQEGERVTIASAAPSSVYREVGPLKFSPKTPNYYPGEPMSLTNHLNGRVSPLIRAPAKNGGLSLYSPTVFFPLLAVFATGDAASGMLDPSLPQIISVAAVSSVAVGATLNGLLPPRLVETVSLRQQLLSQYDTLQSRIKELKEAAENEVWMLARMCQLENKIVAVGEPSYRARRNRVKKVREGLESSLKSRIELIESYARISSMIEIEVEMDSDVLAAESASNAETIADQIEQIMEIENLEERWRIQAEANDEVEKLLNSETLPSQQVSDIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CL24</accession>
    <name>A0AAD5CL24_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_011252</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007732">
        <property type="protein sequence ID" value="KAI7743489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CL24"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051114">
        <property type="entry name" value="Mito_RNA_Proc_CCM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47934:SF27">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47934">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN PET309, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12854">
        <property type="entry name" value="PPR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="15"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="50"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="86"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="121"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="175"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="210"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="245"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="280"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7743489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="356" mass="40174" checksum="12D472118D349DC6" modified="2024-05-29" version="1">MLFKKLIKQKLCEPNVVMYSTMIKGLCKFGNNVIALALLRLMEQKNCKPNVVTYNTIIDSLCKDKMIDDAFNLFEEMVSDKGILPDVITYNSLIHGLCKFGHWDEAKKMLIEMQDQKISPDVQTLNVLVDSFCKEGKIEEANAVINIMVERGKVPNIVTYSSLIDGYCLRGLVPNVFTYNSLLNGYCKNMNIDKAMHVFDEITRKGLKPDVVTYNTMLQGLFQVGRCVAARKLFDEMQAQGLIPNQCTYQIVLHGLCNNHQVEEALSLFQLMGDSKLNSDIVAYTILIDGAGKRGKFDIARNLFHDLINKGLQPDVRIYTVLIAAGSLDTSMVHLLGKLVPKELLDHPNLCDWERN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CM52</accession>
    <name>A0AAD5CM52_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_017308</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007538">
        <property type="protein sequence ID" value="KAI7744428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CM52"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7744428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="86" mass="9799" checksum="2988D137860223D5" modified="2024-05-29" version="1" fragment="single">EPPPDVWDTNNLDAVVFLTATKGMKAIVEKLRDASSLLLLLINMEVKCENFNVAAHVERPFYNRLQKIFTINPYDASEPINVRVLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CMB7</accession>
    <name>A0AAD5CMB7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_003183</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the AP2/ERF transcription factor family. AP2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007802">
        <property type="protein sequence ID" value="KAI7743151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CMB7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00018">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.730.10:FF:000002">
        <property type="entry name" value="AP2-like ethylene-responsive transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.730.10:FF:000004">
        <property type="entry name" value="AP2-like ethylene-responsive transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.730.10">
        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001471">
        <property type="entry name" value="AP2/ERF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32467">
        <property type="entry name" value="AP2-LIKE ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32467:SF237">
        <property type="entry name" value="AP2-LIKE ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00847">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00367">
        <property type="entry name" value="ETHRSPELEMNT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00380">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51032">
        <property type="entry name" value="AP2_ERF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="10">
        <location>
            <begin position="133"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="AP2/ERF" evidence="10">
        <location>
            <begin position="225"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="113"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7743151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="375" mass="41803" checksum="2DCC6B5C252C4C18" modified="2024-05-29" version="1" fragment="single">MLDLNVDVVVSVDEDESCELVTEMEIHSGDSVSSDVLNDSQIANAARFDEEELSRDASNVENCSNGSPVGLITRKLFPVEIDLSSRSVSSSSSVGTTTSLLGSHWLNLKVPEHVPPMQQTKKSRRGPRSRSSQYRGVTFYRRTGRWESHIWDCGKQVYLGGFDTALAAARAYDRAAIKFRGIDADINFSVKEYEEDMTQAKNLSKEEFIHILRRQSTGFSRGSSKYRGVTLHKCGRWEARMGQLLGKKYVYLGLFDNEVEAARAYDKAAIKCNGREAVTNFEPSIYGSDRSLEAMNQGIGDNLDLNLCVSATNDSRKGKDDVRKVDICFKACELPSEKGHKVPARPQAIIGGQTTVSNHYPLWHNMYSGFTPNFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CN62</accession>
    <name>A0AAD5CN62_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Starch synthase, chloroplastic/amyloplastic</fullName>
            <ecNumber evidence="11">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_029837</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[(1-&gt;4)-alpha-D-glucosyl](n) + ADP-alpha-D-glucose = [(1-&gt;4)-alpha-D-glucosyl](n+1) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18189"/>
            <dbReference type="Rhea" id="RHEA-COMP:9584"/>
            <dbReference type="Rhea" id="RHEA-COMP:9587"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15444"/>
            <dbReference type="ChEBI" id="CHEBI:57498"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.4.1.21"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Glycan biosynthesis; starch biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Plastid</location>
            <location evidence="11">Chloroplast</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Plastid</location>
            <location evidence="11">Amyloplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the glycosyltransferase 1 family. Bacterial/plant glycogen synthase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="11"/>
    <dbReference type="EMBL" id="JAMZMK010007429">
        <property type="protein sequence ID" value="KAI7744842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009501">
        <property type="term" value="C:amyloplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009011">
        <property type="term" value="F:alpha-1,4-glucan glucosyltransferase (ADP-glucose donor) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004373">
        <property type="term" value="F:alpha-1,4-glucan glucosyltransferase (UDP-glucose donor) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010021">
        <property type="term" value="P:amylopectin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019252">
        <property type="term" value="P:starch biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03791">
        <property type="entry name" value="GT5_Glycogen_synthase_DULL1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000048">
        <property type="entry name" value="Starch synthase, chloroplastic/amyloplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00484">
        <property type="entry name" value="Glycogen_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011835">
        <property type="entry name" value="GS/SS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013534">
        <property type="entry name" value="Starch_synth_cat_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02095">
        <property type="entry name" value="glgA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45825:SF11">
        <property type="entry name" value="ALPHA AMYLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45825">
        <property type="entry name" value="GRANULE-BOUND STARCH SYNTHASE 1, CHLOROPLASTIC/AMYLOPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08323">
        <property type="entry name" value="Glyco_transf_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0035">Amyloplast</keyword>
    <keyword evidence="4 11" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="6 11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0934">Plastid</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0750">Starch biosynthesis</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Starch synthase catalytic" evidence="13">
        <location>
            <begin position="124"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="12">
        <location>
            <begin position="443"/>
            <end position="594"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7744842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="638" mass="69938" checksum="BB0A5767C2EB865B" modified="2024-05-29" version="1">METLLQSCPVFTSKSLLQSSFDKQFVARSFAFSKHQRTCNVKLRAIGKSSQDGSSSSSVEDVRKDRSVLLGAEKDASGSIVGFHLIPQSGDTKTSNSHDDISNDKELLVDDELADTRPTKVTYNIVFATSEAAPYSKTGGLGDVCGSLPIALASRGHRVMVVTPRYLTGGPSDKKFAGAVDLDCRIKVSCSGGVHEVXXXGVDWVFVDHPSFHRPGNPYGDTYGAFGDNQFRFTLLAHAACEAPLVLPLGGFTYGEKCLFLANDWHTGLVPVYATNSLNILKLLAAKYRPHGVYKDARSIVVIHNLAHQGVEPASTYSNFGLPPEWYGALGWVFPTWARTHALDTGEAVNVLKGAIVTADRILTVSQGYSWEITTPGGGNGLDELLGSRKTVLNGITNGIDINEWNPSLDEHIPSHYSLDDLSGKIECKIALQRELGLPVRPDCPLIGFIGRLDYQKGIDIILSGTPELLQDDVQFIMLGSGEKQYEDWMRATEATFTDKFRGWVGFNVPISHRITAGCDILLMPSRFEPCGLNQLYAMRYGTIPVVHGTGGLGDTVKTFNPFADEGKGEGTGWAFKPLTRESMLDALRVAIETYRKHKSSWLGLMKRGMERDYSWDNAAVQYEQVFEWASIDPPYVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CPZ5</accession>
    <name>A0AAD5CPZ5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_021845</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007473">
        <property type="protein sequence ID" value="KAI7744649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CPZ5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000416">
        <property type="entry name" value="p-loop nucleoside triphosphate hydrolase superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056653">
        <property type="entry name" value="DUF7751"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051701">
        <property type="entry name" value="Mito_OM_Translocase_MSP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45644">
        <property type="entry name" value="AAA ATPASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G12920)-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45644:SF83">
        <property type="entry name" value="P-LOOP CONTAINING NUCLEOSIDE TRIPHOSPHATE HYDROLASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24933">
        <property type="entry name" value="DUF7751"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042073124" description="AAA+ ATPase domain-containing protein" evidence="7">
        <location>
            <begin position="23"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="8">
        <location>
            <begin position="465"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="169"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="370"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="392"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7744649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="683" mass="75184" checksum="BE96021F193DF9DF" modified="2024-05-29" version="1" fragment="single">MEQKHILLSALSVGVGVGVGLGVNKWSNGGVEDGVTAAQIEHELFRLLVDGKDEKDTLKDFPYYLSERTRWLLTSAAYVHLKHLDVSKYTRNLSPASRAILLSGPAEFYHQKLAKALSHDFDAKLLMLDIVNFSLKMQSKYGLKKDSSVKRSISDMALERMSSLLGSFSTAKEDGGGASSKQSGGTAAKSRSVETINHPLKHHRNAAVSSDAGAISSPCPSSNLGSTLVLGSMMMDPDDESTNTKVNDKIASLFPYNIEIKPPEDECHLINWKAKMEEQMKDIQFEENKNHIAEVLAANDLECDDLGSICHADTMFISNYIEEIVVSALSHHLMSEKNPEYRNGKLIISSTSLSHGLSIFQEGYGGKDTLKLETNADSSKGPNGDENAASKSDNKNETEKSVPPKPEVPDNEFEKRIRPEVIPANEIGVTFGDIGALDDIKESLQELVMLPLRRPDLFNGGLLKPCRGILLFGPPGTGKTMLAKAIANEAGASFINVSMSTITSKWFGEDEKNVRALFSLAAKVSPTIIFVDEVDSMLGQRTRVGEHEAMRKIKNEFMSHWDGLLTKPGERILVLAATNRPFDLDEAIIRRFERSGSDLKNLCVTAAYRPVRELMQQEKQKDTEKKSDEEASESVIILRALNMEDMRQAKNQVAASFAGEGCVMGELKQWNELFGEGGSRKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CQF9</accession>
    <name>A0AAD5CQF9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNA helicase</fullName>
            <ecNumber evidence="3">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">M8C21_027071</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">AA19_3_7</strain>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">ATP-dependent RNA helicase required for 60S ribosomal subunit synthesis. Involved in efficient pre-rRNA processing, predominantly at site A3, which is necessary for the normal formation of 25S and 5.8S rRNAs.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DEAD box helicase family. DDX5/DBP2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010007403">
        <property type="protein sequence ID" value="KAI7744950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00268">
        <property type="entry name" value="DEADc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000008">
        <property type="entry name" value="ATP-dependent RNA helicase RhlB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044742">
        <property type="entry name" value="DEAD/DEAH_RhlB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0347">Helicase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Helicase C-terminal" evidence="17">
        <location>
            <begin position="327"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="15">
        <location>
            <begin position="837"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="18">
        <location>
            <begin position="1308"/>
            <end position="1336"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="16">
        <location>
            <begin position="1339"/>
            <end position="1513"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="17">
        <location>
            <begin position="1542"/>
            <end position="1686"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="473"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="997"/>
            <end position="1030"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1070"/>
            <end position="1179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1195"/>
            <end position="1219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1733"/>
            <end position="1806"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1827"/>
            <end position="2048"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="13">
        <location>
            <begin position="1308"/>
            <end position="1336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="18"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="33"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="14">
        <location>
            <begin position="490"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="505"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="14">
        <location>
            <begin position="521"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="14">
        <location>
            <begin position="539"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="14">
        <location>
            <begin position="565"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="608"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="637"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="683"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="997"/>
            <end position="1006"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1108"/>
            <end position="1179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1203"/>
            <end position="1213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="14">
        <location>
            <begin position="1737"/>
            <end position="1752"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="14">
        <location>
            <begin position="1759"/>
            <end position="1773"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="14">
        <location>
            <begin position="1782"/>
            <end position="1806"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1849"/>
            <end position="1872"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1876"/>
            <end position="1896"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1897"/>
            <end position="1915"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1925"/>
            <end position="1945"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1947"/>
            <end position="1966"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="1967"/>
            <end position="1976"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1978"/>
            <end position="2004"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="2005"/>
            <end position="2022"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="2030"/>
            <end position="2041"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAI7744950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="2048" mass="226219" checksum="A67F2635E3A35889" modified="2024-05-29" version="1">MYYWNPETNVTQYEKPTAVPPLVPSGPPSMPSTAQPVSAPSTRTEQPNGIPGQQAHQMFSTPEQQMPPQQGSNISQQYQGINMEPSQGYQFTHNQPMPYVGYQQRPPNMMQPSQHLQQFPQQQDQRAAFPQREVGSHVQNQSSDVNSIQAHPTQYGGSQFNMQQPSPLGQLQQSGVDYQQHGPKFQNQMGQQPNVPPVGLKTSFENNLSGRGGNDQYFNAKNGVPAMTHQPKLAAIPMARTQPDMKSGGLVHQNVSPSFPGGPTSHNMYGQVTGGPPFMNNTMMMANPAVMAPPDAMALSSVEVYRKKHDVTATYVEVVPQMEKQRRVEQILRSEEMGSKIIIFCSTKKLCDQLTRSISRNFGAAAIHGDKSQGERDYVLNQFRSGKSPILVATDVAARGLDIKDVRVVINYDFPNGVEDYVHRIGRTGRAGAKGVAYTFFSEQDWKHAADLIKLLEGANQPVPNEVREIATRGAPGFGKDRGGMSRFDSGGGGGRWDSGGGRGGMRDGGFRGRGFDSRDGGLGGRGGPGGGDMRDGRFGGQRDGGFSGRGGFGGRGGQRDDGFGGRGGGFGGRGGGRDGNFGGRGGGRDGNFGGRGGPRGVGGPDGSWDRNDRFRDSRGGRGRGRFDNRRDDDRGRRRSYSRSRSRSRSYSRSRSRSWSRSRSRSRSRSRSRSRTPVRRERRRESKFDQKAPELPVAGLNSEPGFVQQDVRPENSIALNPVAEPHQMGVIDLHKHGSRKKLVRTLTDETDDVFSNSLSNASFIRFRSNGAVPVPPPTISELRRALWKIGEIEREIRNFENGKGAAVELQNNGFINMDAEGASCYLGPRYAFVPPDPTLPEPWKGLIDGSTGLMYYWNTVTNVTQYEKPTAVQKPMFSMPEQQMRPQQRNGRFQLYQGVNLKQSQSYQVTRDQPMHYMGYQQWPSNMRQPLQRLKQFHQQQDHRAALPQRENGPRVQNKRSNVNLVQTPPAKEGSLVLNRTLDVNPVQTLPARNGTLVINQPSDVNPVQIPPAKRGPLVQNRPSDVKPVQTLPAQKDPLVQNQPSEFNHVQTPTAQKGSLLQNQPLDVNYVQTPPAKKGPLVQNQPLDVNPVNPPPIQNGPPVQNQPSDVNPVQTPPTQNGSLVQNQPSDVNPIQTPPTQNGPLVRNQPSDANPVQTPPTERGSSQFQMQQPWTCGQSQQSVADCQQHDIPPVGLKTGFEDNSPGRDKNDPSFDAKNGGPDMVPHQSKLATIPMANFFPEMNSVDLVHHNVSPTLPGGPNTCGDPTSMNNANPAVIVSPYAMTLSSVDIYRKKHDITAMGDNVPAPFMSFESTGFPPELLREIYAAGFASPTPIQAQTWPIALQNRDMVAIAKTGSGKTLGYLIPAFMLLRRCNNNRQHGPTVIVLSPTKELATQIQNEAIKFGQFVRISCTCLYSGAPEGPQLKDLERGAHIVVASPGRLNDIIEMRKLDFCQVSLLVLDEADRLLAMGYEPQIKKIVNEIPHRRQTLMYTATWTKEVRKLANDLLDTAVQVNIGSEDEHAANKSITQYVEVVPHMEKHRRVEQILRSEERGSKIIIFCSTKKCCDQLTYSISGKFGAAAIHGEKSLGQRDSVLNQFHSGKFPVLVVTDVAAPGLDIKDVRVVINYDFPNGVEDYVRRIGLTGRAGTKGVAYTFFSEQDWKHAADLIKVLEEANQPVPHEVREIARRGCLGFGKDGGGMCHFKSGHWDYGGRGRGFGFGVMRDDGSRGCGVDSRDGGFGSPGRGNMRGGRFGGRDGGFRGCGGRTDGNIGGRGGKRDVNFGGRGGMRDSGGGRDGNFGGRGGHKGVGFGGPNGGCDGNDRFEDNRGGGCGHGRGQFDNSRDGDRSRGRSYSRSPSRSRSWSRGYSKSWSQSHTLVRCERSRESRFDDGRDGDLIRGRSYSRSGSWSSGRSWSHSRSPRRSRTPVRRERRWESRFDRKRDGDHSRGRSYSRSRSWSRNSSRSWSRGHNSRRSRSHTPVRCDRRRESRLDNRRDGDRSMGRDWSRSRSLSRSQSRSWSSSRSQTAVRRERRRESKFDQKEPDVPIPLKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CQH4</accession>
    <name>A0AAD5CQH4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_007288</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007093">
        <property type="protein sequence ID" value="KAI7745867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CQH4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057135">
        <property type="entry name" value="At4g27190-like_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050905">
        <property type="entry name" value="Plant_NBS-LRR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463:SF211">
        <property type="entry name" value="AAA+ ATPASE DOMAIN, P-LOOP CONTAINING NUCLEOSIDE TRIPHOSPHATE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23247">
        <property type="entry name" value="LRR_RPS2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0611">Plant defense</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="4">
        <location>
            <begin position="13"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein At4g27190-like leucine-rich repeats" evidence="3">
        <location>
            <begin position="212"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein At4g27190-like leucine-rich repeats" evidence="3">
        <location>
            <begin position="405"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein At4g27190-like leucine-rich repeats" evidence="3">
        <location>
            <begin position="650"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein At4g27190-like leucine-rich repeats" evidence="3">
        <location>
            <begin position="906"/>
            <end position="1038"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7745867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="1141" mass="130118" checksum="E63297E7B8171C43" modified="2024-05-29" version="1">MTDMPILPLPWSVKSLPKMRTLCLERCKLRDMSSIGELKSLEILSFVGSNVKRLPEEVGQLTQLRLLNLTDCEELTTIPPNVLSKLSSLESLNMMNSFVNWSGEDEEDMQRNNPSLSELKMLPCLRSLEVNILDVSLIPRDLLLENLIKFKIYVGSYWLVSLTHKYSTTLKLRIDEAIISLPDCITLLLKQAQYLILEYCPQVLKNVLYDMRREHFQNLRGLELYGNSDIECIVDTIISKPGALFPSLEKLEVVSMENLKEICHGHLPATSFSALQELDLKYLPKFVCLWKEGTEGDICLVNLRKASVHTCHKLRILFPLRTATDRGLLRYLCVFNCEILQGIFSDNIAVSGEEMGEIVLPMLNTLELSLLPQLKTFCPTLNEMSSSDDNHDMPLFGGNVKFPELEELILRELPKVKSLWHLQVPEESFGRLKVLRITRCNAISKLIPFNMVQRLNNFESLSIINCDLVKEIFEIKGLVDETSAKSVNLPPLKDLVLCGLSELNHLWWNQGSFGYVSLQFLSLLTITRCDSLTYIFSLSALKGLNQLQRLEIESCALVKEIVSFELREDSNMVVFPQLHTLRLYHLPELTSFYKDHKALDWPSLKNLTIANCPQLKTFPASQCEQIAGHVGNLWSTVPPLFNEKVSFPNTEELHIHYMENLVEVWHNKLPDQTFSSLQLLDVRGCERLLDVGPTYMLPRLQMLSKLYIEDCCSVEEIFVTKSALNQDDIHVSLFSLTELRLKSLPKLKHIWWETDSRCGPLHFQNLTSLEVSGCDQLKYILSTSIMKGLAQLQELRISSCELVEEVVLKDISVEDQVEVVSLPRMKTLELKSLPNLKSFCSMNGTLQLSSLQVLKVTNCPMMKMFATFLNQSNLRFENHVSDEFFGREVLLPVLEKLSIEGLSISAGLWHNQLPSNSCHHLNKLKVKRCKNLLRLVPELKDLKSLYVEECDSLEEILGIEQSITGAHENHLVCTLSDVNLQNLPRLAEIWWNKDLNGILRYPMFSSLKVIKCDGLRKVFSASVIKHFLHLEELHLANCLIMKEVVADDERNEYAGEDVVFPQLHSLWLLNLPNLTSLCAGSFTVKFPSLEYLNLEACPDMQMFTLGPLVTPKLNAVVLENGEQFWKDDLNSTIKHLSMAKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CTW5</accession>
    <name>A0AAD5CTW5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_019108</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006718">
        <property type="protein sequence ID" value="KAI7747592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CTW5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.500">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045284">
        <property type="entry name" value="At2g27730-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33878:SF6">
        <property type="entry name" value="F1F0-ATPASE INHIBITOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33878">
        <property type="entry name" value="OS08G0559000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7747592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="75" mass="8846" checksum="84FDEAA222EF589D" modified="2024-05-29" version="1">MAMRSTFISRSHSMVSNSRSALRFLSDQGGRVLEDEERARENLYVKKMEKEKLEKQKQKLEDKEKADKEKSDKKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CUB9</accession>
    <name>A0AAD5CUB9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DYW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_013752</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. PCMP-H subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006918">
        <property type="protein sequence ID" value="KAI7746691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CUB9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000325">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein At4g14820"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000348">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000427">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032867">
        <property type="entry name" value="DYW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF395">
        <property type="entry name" value="TETRATRICOPEPTIDE-LIKE HELICAL DOMAIN, DYW DOMAIN PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14432">
        <property type="entry name" value="DYW_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="186"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="287"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="318"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="419"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="domain" description="DYW" evidence="4">
        <location>
            <begin position="634"/>
            <end position="726"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7746691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="726" mass="81582" checksum="4B4E27DE2AAD6658" modified="2024-05-29" version="1">MNVMTTIPQPIISSTVHVNHYNQLLTTLSAASSLSHLKQVHARIIRLGLDRSNSLLIKLAVSACTLSSPSFDYALSAFYQMHNPEPHLSNKLLRELSRSIKPEKALLAYAKMREKGFVVDSFSLPPLLKASTRIPALSEGMELHGFATKLDFVSDPFVQTGLVAMYSACGHIEDARMMFDKMPERDVVAWNMMIDGYCGNGRYNDVLPLIEEMKRSDVKPDEKIFSTVVSACGRAGNLEFGKAFHEFITENKVVIDYNLQCSLVIMYAGCCSMDMAANIFKKLSPKNIVVSTAMITGYSKAGRIDDARLIFDQMTEKDLICWSAMISGYAERGQPQKALQLFDKMLTLGVKPDQVTMLSVISACASLGAVHNALKIQSYVDENRFSGVLPVNNALIDMYAKCGELDRAKQVFTRVHRRNVITWSSMIGAYAVHGDAINALDLFHEMKNQKTAPNEVTFVGLLYACSHAGLVEEGRKIFASMVNDYNLTPKREHYGCMVDLYGRANLLKEALEVIEQMPMAPNVVIWGSLMAACRIYNETELGEFAAKRVLELDPYHDGAHIFLSNIYAKGKKWENVGEIRKLMQNKGVVKQRGSSRIELDGEIHEFLTADKNHARVDEIYEKLDAVVSELEVAGYTPNMSCVLVDLDEDEKTKALLWHSEKLALCFGLLRRKRGSCIRIIKNLRVCEDCHNFMKLASKVYGIQIVVRDRTRFHRYEDGVCSCKDYW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CVA0</accession>
    <name>A0AAD5CVA0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HNH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_011089</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006422">
        <property type="protein sequence ID" value="KAI7748958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CVA0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004520">
        <property type="term" value="F:DNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031297">
        <property type="term" value="P:replication fork processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00085">
        <property type="entry name" value="HNHc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002711">
        <property type="entry name" value="HNH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003615">
        <property type="entry name" value="HNH_nuc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45766">
        <property type="entry name" value="DNA ANNEALING HELICASE AND ENDONUCLEASE ZRANB3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45766:SF5">
        <property type="entry name" value="SNF2 DOMAIN-CONTAINING PROTEIN _ HELICASE DOMAIN-CONTAINING PROTEIN _ HNH ENDONUCLEASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01844">
        <property type="entry name" value="HNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HNH" evidence="3">
        <location>
            <begin position="61"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="99"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7748958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="156" mass="17729" checksum="4A07DA0A6E4A7B45" modified="2024-05-29" version="1" fragment="single">GLAQIEHGICTKCHLDCRKLVKNLKPLSLEKRREFVIKEAPELAARKNLLEKLINDPTEGNAWHADHIVPVYRGGGECRLENMRTLCIACHAKVTAAQCAERRLERAKAKKQLNRLLANLKTVQDKQQDRDDCRTIDSELLVNVPGSAYHTINRQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CVK3</accession>
    <name>A0AAD5CVK3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_022264</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006478">
        <property type="protein sequence ID" value="KAI7748724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CVK3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000016">
        <property type="entry name" value="Cytochrome P450 78A5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051996">
        <property type="entry name" value="Cytochrome_P450_78A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47946">
        <property type="entry name" value="CYTOCHROME P450 78A7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47946:SF13">
        <property type="entry name" value="CYTOCHROME P450 FAMILY PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8 9" id="KW-0349">Heme</keyword>
    <keyword evidence="6 8 9" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="7"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="8">
        <location>
            <position position="464"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7748724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="492" mass="55741" checksum="0D82CAC33319BA94" modified="2024-05-29" version="1" fragment="single">MKISVKAGFLPLVICFVIGFYNLSLPHLFLISFVSLFPFFVNIWLVPGGFAWRNPNLNPTKIPGPVGWPFLGILPKMGPHAHSKLAFIASSMGATRLMAFSLGSTRVVITSHPDTAKEILCGSAFSDRPIKESASLLMFERAIGFAPSGQYWRHLRRLAANHMFSPKRVSSLECLRQRVCDQMMEKVSKEMSVRGVVELRGILQKGSLRNVIESVFGSGLGLEVEEKLGFMVKEGYELISEFNWGDHFPIRVLDFNGVKRRCHKLSLKVQRLVDQIVEERRRDINTQGTINDRNDFLSVLLSLPEEDQLNHPDMVAVLWEMIFRGTDTVAILLEWIMARMVLHQDIQAKAQDEIKELVGEHRLVQDSDIPNLGYVQAIVKEVLRLHPPGPLLSWARLSTRDVQLGKFFVPAGTTAMVNMWAITHDPSIWKNPWDFKPERFMEEDFPVMGSDLRLAPFGSGRRVCPGKSLGLATVNLWLARLLQQYKWLPSTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CX91</accession>
    <name>A0AAD5CX91_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Beta-galactosidase beta-sandwich domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_028843</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006408">
        <property type="protein sequence ID" value="KAI7749100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CX91"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.455.10">
        <property type="entry name" value="Ribosomal protein S7 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041392">
        <property type="entry name" value="GHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000235">
        <property type="entry name" value="Ribosomal_uS7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036823">
        <property type="entry name" value="Ribosomal_uS7_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11205">
        <property type="entry name" value="RIBOSOMAL PROTEIN S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17834">
        <property type="entry name" value="GHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47973">
        <property type="entry name" value="Ribosomal protein S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Beta-galactosidase beta-sandwich" evidence="4">
        <location>
            <begin position="13"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF17834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7749100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="292" mass="32794" checksum="C379561E637002D0" modified="2024-05-29" version="1" fragment="single">WPDLCLELNIHDCNLPPWSISILPDCKTTVYNTARVGAQTIVMKMTREPTGFAWQSYNNETEYYDDDTFGTVGLLEQLNVTRDASDYLWYMTDNVLIYDFNRVLFSQPVLKFNPNEMFLIRGEQPTLTALSVGHALHVFINGQLLGTVYGSLENPKITFKNVVNLRAGLNKISLLSIAVGLPVGLKGEILSLHSLSGSSSVEWAQCLIIERLTNSLMMHGRNNSKKLMVVRIVNHAMEIIHFGPREDATRIGSTGIVRRHAVDISPLRRVNQSIYLLTTGARKSAFRNVKTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CY22</accession>
    <name>A0AAD5CY22_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Probable purine permease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_007588</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the purine permeases (TC 2.A.7.14) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006526">
        <property type="protein sequence ID" value="KAI7748430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CY22"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005345">
        <property type="term" value="F:purine nucleobase transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015211">
        <property type="term" value="F:purine nucleoside transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030182">
        <property type="entry name" value="PUP_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31376">
        <property type="entry name" value="OS09G0467300 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31376:SF2">
        <property type="entry name" value="PURINE PERMEASE 11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16913">
        <property type="entry name" value="PUNUT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="26"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="60"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="123"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="154"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="187"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="220"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="265"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="298"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="321"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU368015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7748430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="364" mass="39756" checksum="1E687A36483F9C87" modified="2024-05-29" version="1">MPDNEDSVVVTRPGVLPLAKLKRWQWWVLVAFNICFLLIGQIAAVLLGRFYYDQGGNSKWMATLVQTIGFPILFIPYFLFPLITTSSTTPTTSYSLPVIFLLYIVVGVLIAGDNMLYSVGLLYLSASTYSLICATQLAFNAVFSYFINSQKFTALIFNSVVVLSLSASLLAVNDDSDKPSGVSDKNYALGFVATLGASALYALLLSIMQLSFQKVIKKETFSVVLELQIYTSFFATCVSTIGLFASGEWRTLSGEMGSFGKGGLAYVMTLVWTAVAWQVCSVGVVGLIFVVSSLFSNVISTLSLALTPLAALVVFHDKMNGVKLIAMLLGLWGFCTYIYQNYLDDLKAKREETDANGMQLKSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D0T6</accession>
    <name>A0AAD5D0T6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">PRA1 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_021224</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 7">May be involved in both secretory and endocytic intracellular trafficking in the endosomal/prevacuolar compartments.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 7">Membrane</location>
            <topology evidence="2 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the PRA1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006235">
        <property type="protein sequence ID" value="KAI7750006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D0T6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004895">
        <property type="entry name" value="Prenylated_rab_accept_PRA1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12859:SF0">
        <property type="entry name" value="PRA1 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12859">
        <property type="entry name" value="PRA1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03208">
        <property type="entry name" value="PRA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="52"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="77"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7750006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="197" mass="22492" checksum="3AA7FCDCF0BFFD24" modified="2024-05-29" version="1">MDWATVSTEDLIDALKEVDWSSPPRPINEFFNKFTFPRSSNKWNSRLKCNLYYYRTNYFLMILFILGLGFLRRPLAIVAAVSTALTIAFLNDSFAGTFSEKLTRFVRQFSPHLAAKMRPPPMPVLRGRPSSKRAIYICGRPRWQFVIMSSAVSLFLWFVSCGLLTLLWALAVGLLDLLIAWFFKAELPIDPAVAVAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D118</accession>
    <name>A0AAD5D118_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_009199</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010005766">
        <property type="protein sequence ID" value="KAI7752018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D118"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd14016">
        <property type="entry name" value="STKc_CK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055900">
        <property type="entry name" value="DUF7477"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24289">
        <property type="entry name" value="DUF7477"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="35"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7752018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="532" mass="59444" checksum="6D14F92B8C895B85" modified="2024-05-29" version="1">MDYYDSAGLSDDEVLGNEGHTPVPETVNVGGSTSYKVERELGKGGFAQVYLGRRINGQLSNGSTAVEVALKFEHQSSNGCIYGPPYEFHVYDDVSGSHGVPHVYYKGRQGDYYIMVMDMLGPSLMDVLRNNSHSMSIEMVASVAIEAISILEKIHSKGYVHGDVKPENFLLGLPGTSDEKKLFLVDFGLAVRWRNALSGLHVENDQQPYMFRGTSWYASVHAHLGGTLSRRDDLESLAYTLAFLLRGLLPWQTSLGKNKSFLMCKKKMETSPETLFCSCPVPFRKFFEYVVNLKFEEKPNYEKYISLFDGIVGPDPPPINTDGAQKLIVRKRGALSLEQDDDDAEQPKKKVPMGMPVTQWIWVYNACCPMKQIYHHDVADVRLAQLIQKGNEDGLLISSVASCSNLWTLIMDSGTGFTSQVYRVSPLSNNKDWIMEQWKNGYSISAIAGTNDGSSLIVMSKGTPYILQAIIVSEKFQFEWIDKKWKLGYNITAMATEGNKWVVVMSLGSGFSDQVLFAAAIPQSCIRYYIIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D2G1</accession>
    <name>A0AAD5D2G1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">D-alanine--D-alanine ligase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_002702</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005743">
        <property type="protein sequence ID" value="KAI7752162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D2G1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008716">
        <property type="term" value="F:D-alanine-D-alanine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000028">
        <property type="entry name" value="D-alanine-D-alanine ligase family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000291">
        <property type="entry name" value="D-Ala_lig_Van_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011095">
        <property type="entry name" value="Dala_Dala_lig_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011127">
        <property type="entry name" value="Dala_Dala_lig_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23132">
        <property type="entry name" value="D-ALANINE--D-ALANINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23132:SF0">
        <property type="entry name" value="D-ALANINE-D-ALANINE LIGASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07478">
        <property type="entry name" value="Dala_Dala_lig_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01820">
        <property type="entry name" value="Dala_Dala_lig_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00843">
        <property type="entry name" value="DALA_DALA_LIGASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00844">
        <property type="entry name" value="DALA_DALA_LIGASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="D-alanine--D-alanine ligase N-terminal" evidence="1">
        <location>
            <begin position="73"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="D-alanine--D-alanine ligase N-terminal" evidence="1">
        <location>
            <begin position="372"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="domain" description="D-alanine--D-alanine ligase C-terminal" evidence="2">
        <location>
            <begin position="684"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7752162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="792" mass="86444" checksum="ABE1D324AFDE7BF1" modified="2024-05-29" version="1" fragment="single">MRGDLASIYKSDDGTESADIIYPASVVLNRKNWRRNCFRGVGTVHACTEAVAVDDRTVEGVRETKREKGKVLKVGLVCGGPSAERGISLNSARSVLDHIQGDDLHVSCYYIDPNLNAYAISSAQVYSNTPADFDFKLESVAQGFDTLTEFAEHLAASVDIGSELDESGLSRWFLDNQLDVNSGKVVGIDDKVIVEIFLEGGKEFTAIVLDVGSEFDCQPVVFLPTEVELQSSVVYHTPPRFPLDVTEKIREGASILFKRLGLRDFARIDGWFLPPSASITSSETNDFGRTESGIVLFTDINLISGMEQTSFLFQQASKVGFSHSNILRTIIQRACLRFPSLASYCSESKGTPRKSNSSNFGQQTSENEGVRKVFVIFGGDTSERQVSLMSGTNVWLNLQVSGDVEVTPCLLAPGNDVSSRTVWSLPYSLVLRHTTEEVIDACTEALEPARAALTSHLRKQVMEELASGLKSHNWFTGFDISDEQPIKFTLENWIKLAKEVQATVFIAVHGGIGEDGTLQSLLEAEGVPFTGPGFSASKICMDKVATSLAVKHLTNLGVLTINKEVKRKEDLLNSSISDVWHEVVSKLQCETVCIKPARDGCSTGVARLCCAEDLGVYVKALEDCLPRIPPNSFAKAHGLIEMPVPPPELLIFEPFIETDDITINVSKSGNQNEGLLWKGSSRWVEITVGVIGKRGSMRSLMPSVTVKESGDILSLEEKFQGGTGINLTPPPLTIMSTEALMKCKERIELIANTLELEGFSRIDAFVNVDSGEVLVIEVNTVPGMTPSTVLIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D3Q9</accession>
    <name>A0AAD5D3Q9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_015878</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005665">
        <property type="protein sequence ID" value="KAI7752527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D3Q9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025886">
        <property type="entry name" value="PP2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32278">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32278:SF135">
        <property type="entry name" value="F-BOX PROTEIN PP2-B12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14299">
        <property type="entry name" value="PP2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7752527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="489" mass="57356" checksum="371BD58717459359" modified="2024-05-29" version="1">MESFLGEFQHLRIQLEDIKSATENFDNKRLIGVGGFGKVYKGELCHFKGRSMVAFKRLDRKFGQGDPEFWKEWFSLNKTGENCEMISAAECIFPKEYIPDEDNDPGYVSRKISRFPPGNCYKTGSWGFTGHVRTQFLSPHITYTVNLVFKDTDEVLVPTEQRYVCLRYKMKEEQTNSGGWMMADVREDGWMMAELFQFESEKRSVDLEIGFTPYFGEMKGWRSYVIEGIEFRPIEKLEHVMLDDNEDMHTISETDETYWENKLPNDYEKIIKWSEDDVKWTTKKELYFTLCKGFQMSSGQVWLSLDKNGKRCLMRPARYALIAKEWKWKSHAESRFKDVAYSPVHPFSIVCKINSGILSPETKYACYLVYKLEKSPEVLSNFEFVMQVRENFDRTTDWRLQDDNCRFIYIFNPQTPVIRPKAGENSHNPMNRPKLKGIPKQRNDGWMEVQVWEIQTGTTIEIISMRFGLGTGYYGYVTLSVQGIELRPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D471</accession>
    <name>A0AAD5D471_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_015556</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010004938">
        <property type="protein sequence ID" value="KAI7753991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D471"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005540">
        <property type="entry name" value="KNOX1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005541">
        <property type="entry name" value="KNOX2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48452">
        <property type="entry name" value="FUSED COMPOUND LEAF 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48452:SF1">
        <property type="entry name" value="FUSED COMPOUND LEAF 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03790">
        <property type="entry name" value="KNOX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03791">
        <property type="entry name" value="KNOX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01255">
        <property type="entry name" value="KNOX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01256">
        <property type="entry name" value="KNOX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KNOX1" evidence="3">
        <location>
            <begin position="11"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="domain" description="KNOX2" evidence="4">
        <location>
            <begin position="62"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7753991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="119" mass="13512" checksum="E00C91F18A3A2D47" modified="2024-05-29" version="1" fragment="single">MQSNGYDDDGDSIKEKIASHPSFLKLLDAYIDCQKVGAPQEIASLLDNIRQENYVRKRNATVSTCLGVDPELDEFMETFYVLLVKYKSELRRPFDEAATFIGNIKTQLRNLSIGKFIST</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D582</accession>
    <name>A0AAD5D582_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cystatin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_008268</name>
        <name evidence="6" type="ORF">M8C21_008277</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005787">
        <property type="protein sequence ID" value="KAI7751946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAMZMK010005787">
        <property type="protein sequence ID" value="KAI7751955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D582"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004869">
        <property type="term" value="F:cysteine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00042">
        <property type="entry name" value="CY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000010">
        <property type="entry name" value="Cystatin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046350">
        <property type="entry name" value="Cystatin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018073">
        <property type="entry name" value="Prot_inh_cystat_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47373">
        <property type="entry name" value="CYSTEINE PROTEINASE INHIBITOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47373:SF1">
        <property type="entry name" value="CYSTEINE PROTEINASE INHIBITOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16845">
        <property type="entry name" value="SQAPI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00043">
        <property type="entry name" value="CY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54403">
        <property type="entry name" value="Cystatin/monellin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00287">
        <property type="entry name" value="CYSTATIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0789">Thiol protease inhibitor</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042441280" description="Cystatin domain-containing protein" evidence="3">
        <location>
            <begin position="21"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="Cystatin" evidence="4">
        <location>
            <begin position="20"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7751946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7751955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="121" mass="13408" checksum="2808AB5743428BF1" modified="2024-05-29" version="1">MSKLPISTIIFLILFSSTLAIPGGRTKVKNVKTDTEIQKLGSYSVDEYNRLQRTHKTGDGDLKFSQVVAAETQVVAGTKYYLKIEAVTKKGVVKVFDAEVVVQPWKHSKKLLGFKPAPVNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D667</accession>
    <name>A0AAD5D667_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Bidirectional sugar transporter SWEET</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_009597</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Mediates both low-affinity uptake and efflux of sugar across the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the SWEET sugar transporter family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010004554">
        <property type="protein sequence ID" value="KAI7754082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D667"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051119">
        <property type="term" value="F:sugar transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.290:FF:000001">
        <property type="entry name" value="Bidirectional sugar transporter SWEET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.290:FF:000002">
        <property type="entry name" value="Bidirectional sugar transporter SWEET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.290">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047664">
        <property type="entry name" value="SWEET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004316">
        <property type="entry name" value="SWEET_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10791:SF159">
        <property type="entry name" value="BIDIRECTIONAL SUGAR TRANSPORTER SWEET5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10791">
        <property type="entry name" value="RAG1-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03083">
        <property type="entry name" value="MtN3_slv"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="45"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="69"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="102"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="132"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="163"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="191"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU910715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7754082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="236" mass="26108" checksum="86DE6280A38C8F4D" modified="2024-05-29" version="1">MDSDALRTLVGILGNIIALVLFLSPVPTFVTIVKAKSVQAFRPDPYVATILNCGMWMFYSLPIVRPDSLLLFTINCAGFTIEIVFIAIFLTYSTWGGRKKIIILLSIEAVFAAAIVVVTLTCFHTNASRSMFVGLICLFFNILMYAAPLTVMKMVIKTKSVKYMPLGLSIATFASSIVWSVYAILKYDICLLIPNALGTLFAIVQLILYATYYGSTNWDDVDYKQSEVQMPSTSNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D683</accession>
    <name>A0AAD5D683_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">3-dehydrosphinganine reductase</fullName>
            <ecNumber evidence="11">1.1.1.102</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">3-ketodihydrosphingosine reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">3-ketosphinganine reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">M8C21_012602</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">AA19_3_7</strain>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>sphinganine + NADP(+) = 3-oxosphinganine + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22640"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57817"/>
            <dbReference type="ChEBI" id="CHEBI:58299"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.102"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.102" evidence="11"/>
    <dbReference type="EMBL" id="JAMZMK010004258">
        <property type="protein sequence ID" value="KAI7754184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D683"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047560">
        <property type="term" value="F:3-dehydrosphinganine reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006666">
        <property type="term" value="P:3-keto-sphinganine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08939">
        <property type="entry name" value="KDSR-like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000165">
        <property type="entry name" value="3-ketodihydrosphingosine reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045022">
        <property type="entry name" value="KDSR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43550">
        <property type="entry name" value="3-KETODIHYDROSPHINGOSINE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43550:SF3">
        <property type="entry name" value="3-KETODIHYDROSPHINGOSINE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="260"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="286"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00026112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00050489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00081952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00083783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAI7754184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="323" mass="35233" checksum="E4F8E5C9AE23E6B6" modified="2024-05-29" version="1">MSGNDLSLLTLLIPLLIFLKLIVRPRAVKIPIKSRHVFITGGSSGIGLAIARQAVAEGARVTILARDVNRLEQAKASIRASTGIEVDILSADVRDFEAVKKAVESSSPIDVLICNHGVFSPYELVNQEMKVIKGIININLVGSFNMVKAALTDMKNRSDRRPVSIAFMSSQSGQVGIYGYTAYAASKFGLRGFAEALQQEVISDDIHVSLIFPPDTDTNSLVEENKKRPPLTKLIAASSGQVMNVDKVAKIALNGIKSGTFFVPCNFMGFWLAVGTAGASPQRSCLMAFVEVVFTGVLRIVALFFQWSWYRSIEKWHAKRKSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D836</accession>
    <name>A0AAD5D836_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Glutamate receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">M8C21_030933</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">AA19_3_7</strain>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Glutamate-gated receptor that probably acts as non-selective cation channel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010002863">
        <property type="protein sequence ID" value="KAI7754654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D836"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015276">
        <property type="term" value="F:ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13686">
        <property type="entry name" value="GluR_Plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000172">
        <property type="entry name" value="Glutamate receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000188">
        <property type="entry name" value="Glutamate receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015683">
        <property type="entry name" value="Ionotropic_Glu_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001320">
        <property type="entry name" value="Iontro_rcpt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017103">
        <property type="entry name" value="Iontropic_Glu_rcpt_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18966">
        <property type="entry name" value="IONOTROPIC GLUTAMATE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00060">
        <property type="entry name" value="Lig_chan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037090">
        <property type="entry name" value="Iontro_Glu-like_rcpt_pln"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00079">
        <property type="entry name" value="PBPe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 13" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 13" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 13" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="8 13 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 17" id="KW-0732">Signal</keyword>
    <keyword evidence="4 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 13" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042137319" description="Glutamate receptor" evidence="17">
        <location>
            <begin position="31"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="546"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="576"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="610"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="788"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor C-terminal" evidence="18">
        <location>
            <begin position="426"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="836"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="715"/>
            <end position="770"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR037090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR037090-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAI7754654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="861" mass="97327" checksum="FF994B433AF589B3" modified="2024-05-29" version="1">MHNVYNHLILSLVASSLTCFLLLQSTLVTGSLTSVSKGGNETGIGVILDQTSRPGKEAKVAIELAIQDFNIKTDQPLVLYVKNSRNKPVHAVIAAKELIEEHRVKAILGAHTWEEASTIAEVIDDNKHDHDTPLFLSLAGTTQLQPTDQWPFYIQSVPTQSTQMNAVAAILQSMGIRQVTLIYETPSYVSIISHLTQALHQTGSQLTNILPLTSSSCCLDKELEVVKRQQHKVFVVHTSFELGVRLFKTAKKMDMIGEGYLWIATNAITDLFHSVNSNVISSLKGMVGVKSYFPENTQEFLHFRKRFRRKFRLDYPEDEQDEPGIFAVQGYNAMKMVEKASLEKSNELKTIQANTIEIVNVNGKGYHSVYWTKGSGFTETVENEITGASNYTHLIDNVGHILWPVQPWYASRRRRNLAESSQNRMRVGVPARSLFRQFVRVDPDPEIKDEHVFDGFVVEVFDEMMTAMNQPYDRIPFYGSYDDLIKEIHLKKFDAVAGDVTILSDRHEFADFTQPYTESGLEMIVPVRSRLSNQPWLFLKPFTAKMWWITAAVTIYNGFVIWLIERNHNKDLQGPHPISIITQIGIILWLAFSTLFTLRGDKMSSNLSRMAVVMWLFVALIITQSYTASLASMLTAQRLEPTITSVEMLRSMNATVGYCNGSYINRYLEKVLEFKDFRIKSYNSTHGYAAGLNSGEIKAIFLEVPAAKVFLAQYCKSFIRTGETFKVGGFGFAFHKGFERLSEANAALMNVSESGKLKELEDKHLISEECVDEESSPDENGRLNLHSFWVLFVLTGGTSTVALVIYIVINIKEFKTSDEEDIAFFKHLRRQMRRSSSTVASVESARHTRNIDQVDNTDDDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D949</accession>
    <name>A0AAD5D949_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_031663</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010001074">
        <property type="protein sequence ID" value="KAI7755489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D949"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007096">
        <property type="term" value="P:regulation of exit from mitosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.900.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009511">
        <property type="entry name" value="MAD1/Cdc20-bound-Mad2-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053729">
        <property type="entry name" value="MAD2L1BP_domain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15681">
        <property type="entry name" value="MAD2L1-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15681:SF1">
        <property type="entry name" value="MAD2L1-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7755489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="62" mass="7192" checksum="423CE19EC076B500" modified="2024-05-29" version="1">MQIVPMRLRIKCRNQDRVMHSLNCDTQPANPSNMLDSTSNDYIWFQCRHVIKGLACKASPEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D956</accession>
    <name>A0AAD5D956_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">peroxidase</fullName>
            <ecNumber evidence="4">1.11.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">M8C21_019221</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">AA19_3_7</strain>
            <tissue evidence="21">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 a phenolic donor + H2O2 = 2 a phenolic radical donor + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:56136"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:139520"/>
            <dbReference type="ChEBI" id="CHEBI:139521"/>
            <dbReference type="EC" id="1.11.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="16">Binds 2 calcium ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
        <text evidence="16">Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peroxidase family. Ascorbate peroxidase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.7" evidence="4"/>
    <dbReference type="EMBL" id="JAMZMK010003606">
        <property type="protein sequence ID" value="KAI7754431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D956"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140825">
        <property type="term" value="F:lactoperoxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00693">
        <property type="entry name" value="secretory_peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.420.10:FF:000001">
        <property type="entry name" value="Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.520.10:FF:000008">
        <property type="entry name" value="Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.420.10">
        <property type="entry name" value="Peroxidase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002016">
        <property type="entry name" value="Haem_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000823">
        <property type="entry name" value="Peroxidase_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019794">
        <property type="entry name" value="Peroxidases_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019793">
        <property type="entry name" value="Peroxidases_heam-ligand_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033905">
        <property type="entry name" value="Secretory_peroxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31517">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31517:SF48">
        <property type="entry name" value="PEROXIDASE 16-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00141">
        <property type="entry name" value="peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00458">
        <property type="entry name" value="PEROXIDASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00461">
        <property type="entry name" value="PLPEROXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00435">
        <property type="entry name" value="PEROXIDASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00436">
        <property type="entry name" value="PEROXIDASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50873">
        <property type="entry name" value="PEROXIDASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 16" id="KW-0106">Calcium</keyword>
    <keyword evidence="12 18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0349">Heme</keyword>
    <keyword evidence="13" id="KW-0376">Hydrogen peroxide</keyword>
    <keyword evidence="11 16" id="KW-0408">Iron</keyword>
    <keyword evidence="8 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="19" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042292354" description="peroxidase" evidence="19">
        <location>
            <begin position="19"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="Plant heme peroxidase family profile" evidence="20">
        <location>
            <begin position="19"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="14">
        <location>
            <position position="60"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="16">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="244"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="17">
        <location>
            <position position="56"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="29"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="62"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="194"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="21">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="21">
        <location>
            <position position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAI7754431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="273" mass="30564" checksum="80931628DEFAED4C" modified="2024-05-29" version="1" fragment="single">KQLLCLLFLSFYVTSSYSQLSHNFYQHICPNVESIVRSAVTEKFRQTFVTAPATLRLFFHDCFVRGCDASVFLSHAKAEKDHHDDLSLAGDGFDTVIKAKAAVDSNPSCRYKVSCADILALATRDVVALTGGPSYKVELGRRDGRNSTRKSVQHKLPRPDFNLNQLNTMFASHGLSQTDMIALSGTHTLGFSHCGHFSKRIHSKDGIDRTLNRQYALQLKQMCPVNVDPRLVIDMDPTTPHTFDNAYYKNLQQGKGLFSSDQVLFTDARSRPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DBY5</accession>
    <name>A0AAD5DBY5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RecQ-mediated genome instability protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_009973</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RMI1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000275">
        <property type="protein sequence ID" value="KAI7756742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DBY5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016604">
        <property type="term" value="C:nuclear body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031422">
        <property type="term" value="C:RecQ family helicase-topoisomerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000712">
        <property type="term" value="P:resolution of meiotic recombination intermediates"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.770">
        <property type="entry name" value="RecQ-mediated genome instability protein Rmi1, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042470">
        <property type="entry name" value="RMI1_N_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013894">
        <property type="entry name" value="RMI1_OB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14790:SF15">
        <property type="entry name" value="RECQ-MEDIATED GENOME INSTABILITY PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14790">
        <property type="entry name" value="RECQ-MEDIATED GENOME INSTABILITY PROTEIN 1 RMI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08585">
        <property type="entry name" value="RMI1_N_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01161">
        <property type="entry name" value="DUF1767"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RecQ mediated genome instability protein 1 OB-fold" evidence="4">
        <location>
            <begin position="85"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7756742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="273" mass="30279" checksum="DE25CEB48110466F" modified="2024-05-29" version="1">MSNESMQIDEEEWISESSKPVSKSEGPQHISAYFENRFKLNLKEEWVNSLLSCLVTETIGVCERNIFYKKVLDCDLEKICSTGSLPADVKDMSNGFLEGPFLLQVDEIVDISQPLPDRYKNVPSDGVDSLLKLSMTDGGQRVFGLVCQRIEGLHTSASSGLKVAISNATVRRGIIMLHKEVLTVLGGSVKVLDAAHKRIVDELNMPQDYARYTVDSNMWTVDTRRSYAQFINQSCSCSTAGATSGSGSQEKKDLKLKGHAYLNVYRINLGKNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DCJ8</accession>
    <name>A0AAD5DCJ8_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_000303</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000242">
        <property type="protein sequence ID" value="KAI7756870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DCJ8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005673">
        <property type="term" value="C:transcription factor TFIIE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039997">
        <property type="entry name" value="TFE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13097:SF7">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13097">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIE, ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7756870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="125" mass="13884" checksum="9438AC7F293AD459" modified="2024-05-29" version="1">MLASQDIGDGDDNARRRRREKLRDLLQKMEVELKPLTDQLSRVKDLEAPDYGTLQAWELRASAVARASNSVPIGNDSFRSGQGGTPMPFLGETKVEVAFSGVEDKGEIKSEIAPMKVLPPWMIKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G1K1</accession>
    <name>A0AAD5G1K1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_016960</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012227">
        <property type="protein sequence ID" value="KAI7724406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G1K1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7724406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="84" mass="9192" checksum="41911B3A18DD93A4" modified="2024-05-29" version="1">MSTMRRDPDKLPSTTAVNPAHQASTMKNVHINAMTKSKNFGKGKGKEAESSQQREDQGLGSIIKMTELYEQFVGFYGGPPGPSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G1P0</accession>
    <name>A0AAD5G1P0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Mediator of RNA polymerase II transcription subunit 31</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_026058</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the Mediator complex subunit 31 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011990">
        <property type="protein sequence ID" value="KAI7725260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G1P0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1340:FF:000001">
        <property type="entry name" value="Mediator of RNA polymerase II transcription subunit 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1340">
        <property type="entry name" value="Mediator of RNA polymerase II, submodule Med31 (Soh1)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038089">
        <property type="entry name" value="Med31_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008831">
        <property type="entry name" value="Mediator_Med31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13186">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05669">
        <property type="entry name" value="Med31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0010">Activator</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 7" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="132"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="153"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="186"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7725260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="198" mass="22911" checksum="2BEF2C49D77CDAF7" modified="2024-05-29" version="1">MASGQQIDDSSNNNSSPKKVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYFEDEAFIGYLKYLQYWQRPEYIKYIMYPHCLYFLELLQNASFRNAMAHPANKELTHRQQFYFWKNYRNNRLKHILPRPLPEPTAAAPSNALPPPLPPTATTMAASSTPVSAPPLPSPMQYGVPSGPPLPKNDPRNAIDRRKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G1S6</accession>
    <name>A0AAD5G1S6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF985 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_028994</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012208">
        <property type="protein sequence ID" value="KAI7724513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G1S6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06121">
        <property type="entry name" value="cupin_YML079wp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009327">
        <property type="entry name" value="Cupin_DUF985"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039935">
        <property type="entry name" value="YML079W-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33387:SF3">
        <property type="entry name" value="DUF985 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33387">
        <property type="entry name" value="RMLC-LIKE JELLY ROLL FOLD PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06172">
        <property type="entry name" value="Cupin_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042006245" description="DUF985 domain-containing protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="DUF985" evidence="2">
        <location>
            <begin position="32"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7724513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="214" mass="24316" checksum="854973AB661941DC" modified="2024-05-29" version="1">MEVAIKFCMFLVVYFINGVQSIDHHKTLTTSEIIATLNLEPNPESGFYAETFRDTSINISTSQLPPRFKVDRPISTAIYFFLPSGNVSNLHRIPSAETWHFYLGEPITIFEIDDKDGSVKFTYMGKDILESERLQYTVPPDVWFCAFPTRDYDVLADNRVVKKAPRDTEIHFSLVGTTVAPAFEPDDFVLAKRSQLISQFPALESLISMFTPSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G1U0</accession>
    <name>A0AAD5G1U0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_018881</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012115">
        <property type="protein sequence ID" value="KAI7724962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G1U0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33625:SF11">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33625">
        <property type="entry name" value="OS08G0179900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7724962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="344" mass="38006" checksum="DD4AA7B2CC1ED9AF" modified="2024-05-29" version="1">MRRSFGPKMGGGGGGGGGGMMKTVHRAMRAGIGSNTSQEPSYSSHAATGTTSNRNHQHINTTLSLNRPSSPCSYLNHPAPDMPPHNLALTTSLSSATGEEFDWEYVDVEGDGVDGFYDEFVFGGGVPSEQEVHHAVTSLYEVLEPVSFDQLMNHRETYEDDARVDIISSSTGFHNSGLELDWHEPSMQQCYSTSTLQVPRSDKVCDAFHLLQTDPSVQRMVISLSSDKAVWDAVMNNEVVRELRESIREDKSIYDGSEGGVNGSNPVTQVLHWIFANTKDKVIEIVAKITKVVNELLRPMSKDEKPNIDTNVGLDSFDEKLRRSFFLSIVVLLIVVVSRSRKQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G253</accession>
    <name>A0AAD5G253_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Disease resistance protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_001366</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012132">
        <property type="protein sequence ID" value="KAI7724931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G253"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14798">
        <property type="entry name" value="RX-CC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056789">
        <property type="entry name" value="LRR_R13L1-DRL21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038005">
        <property type="entry name" value="RX-like_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766:SF47">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766">
        <property type="entry name" value="PLANT BROAD-SPECTRUM MILDEW RESISTANCE PROTEIN RPW8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25019">
        <property type="entry name" value="LRR_R13L1-DRL21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0611">Plant defense</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="8">
        <location>
            <begin position="12"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="7">
        <location>
            <begin position="198"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="9">
        <location>
            <begin position="425"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="domain" description="R13L1/DRL21-like LRR repeat region" evidence="10">
        <location>
            <begin position="583"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="829"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="835"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF25019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7724931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="942" mass="107977" checksum="3D6EA8B0904252FB" modified="2024-05-29" version="1" fragment="single">QIDLRRAVAALVKEVVGRLTSELIKEFRLFRGFKNDILSLKEEFTQNEAVLEDAEEKQNKDKAVQVWLKSLKSATMEAENVLDEISTRSLLQRLHKEMEIERGIKYKVRALFSKVMFRVKAAHKVKAIRAKLDDIASVRRNLGLNPGDSVDEDSAGEMPNRETSSLVIRDSASIFGRDEEREMVVENICNNGIGKYDNGEILVYGIWGMGGIGKTTLAQLVYDHENVKQNFPLRCWVYVSENFEVNEIIKKIIESVDGCECTLTQLDTLQVKLQDRLTGKKFLIVLDDIWIEDKEKAKWDELSKTLSCGEEGSIVVMTTRSRTTSQMMAKVGKLQHELECLSEDNSWLLFSKFAFAQGREGDDIGVLEPIGREIVEKCKGLPLALKTLGSLMWLKKSTSDWEHMKGSIIWEFQENGVLPALKLSYEMEKDELIHLWVVNGFIPPIEGKALYMFGEEILNYLVWRSFFQLVDTKNHLRNDPYIYKMHDLIHDLALHVMGDDCLVIESTVLPESVIHLQNLQVLILSHCYRLAELPEGLRHMVNLQCLDISDSYALRRLPVGIKELNRLRILSEFPIVKEDGARIGELGDLVLLEGMLEIIGLENVEGFSEAKSANLKCKTNLSILKLRWNSPESILGGYKCDEKEVLEGLEPNPGLKVLEIFCYMDKIISPSWMANLKNLIKITFYYCKKCERIPPLGRFPNLRVIILYRMDCLKGFHEDDTNMSGDNPNIFPSLQTLDIIECHSLVSLPSILPKLKVLKLSRCDQLVSLWDEILKNLNKLYVYRCKHLIKRYKQEIGEDWPKVSHIPDVEIVPAPSYDEDEDDVDDVEFVPAPSYDEDKDDVDNEDEDGDNPNIFPSLQTLRIEYCRSLVSLPSNLPKLGDLQLDSCNVLVSLPEEILKDLNKLHIIECKHLTKRYKHGQDWPKFSHIPDVKFIYYGRVVQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G3R3</accession>
    <name>A0AAD5G3R3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_006416</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011445">
        <property type="protein sequence ID" value="KAI7727092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G3R3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042268021" evidence="1">
        <location>
            <begin position="19"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7727092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="62" mass="6648" checksum="5EBAFF067978B9DF" modified="2024-05-29" version="1" fragment="single">GMVLKPLVTVLLSNIASANDDVQRVQHRQCSVAAPDIFSDVFENDIVPTCVFCPVSVGNVMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G453</accession>
    <name>A0AAD5G453_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_011398</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011475">
        <property type="protein sequence ID" value="KAI7726973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G453"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052636">
        <property type="term" value="F:arabinosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052325">
        <property type="term" value="P:cell wall pectin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053250">
        <property type="entry name" value="Glycosyltransferase_77"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46936">
        <property type="entry name" value="ARABINOSYLTRANSFERASE XEG113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46936:SF1">
        <property type="entry name" value="ARABINOSYLTRANSFERASE XEG113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="13"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7726973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="414" mass="46763" checksum="4D46CBD8349D8B2A" modified="2024-05-29" version="1">MDHGLNESEPRKSGSFSHISSTNSSFRRRSFNRFGSSHHEIDDDSVSEAGDIGDRALSSRRYSESGRPRFSFDNTGENVGLPIQEHPFKDAQIPTASPASPGAILLGKDQNEECKKELPWYLTYISSRVHLAVLGILGVLTRYLLEKLFGPQVVGATGDHSYMYIDLPPNMAMYQQLRLEPYAVHTTFQYAGTEGKRHRLREAMVFYDPPEYYDSPGGFLTFKPSIPKSLLLDGEHNLETHFTLVNYQIKQIRTALAIASILNRTLVMPPVWCRLDRLWFPHPGVITGSMTRQPFICPLDHVFEVHTMLKALPIEEFGPGINIREYSFFDNPLMPAQVKDSWLDVQLCQGGSENCQSNITNTTGVLRFPKNSSEETLKKVLSPLKDVKVIQFSSMQDAFLGFTDKASNLFLFIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G5M2</accession>
    <name>A0AAD5G5M2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_012051</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010966">
        <property type="protein sequence ID" value="KAI7729609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G5M2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052124">
        <property type="entry name" value="Rab9_kelch_effector"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46647">
        <property type="entry name" value="RAB9 EFFECTOR PROTEIN WITH KELCH MOTIFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46647:SF1">
        <property type="entry name" value="RAB9 EFFECTOR PROTEIN WITH KELCH MOTIFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7729609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="370" mass="41458" checksum="0A003EBEE627DF0A" modified="2024-05-29" version="1" fragment="single">WIKIVKDQYCRIEHSKLGLLRPHLDKTLPKYYLKNIMEVFCKGFSNKYKTFLRLHDLASAASHYESLYRGNAELGRLSAKCHTKVSSCSNFLFINFVDNKLWFQPECTGSGSEGQTAPSPRAFHIAIAIDCHMFVFGGRLGTKRLGDFWVLDTDIWQWSELTSFGDLPSPRDFAAASAIGNQKIVMSELLQKLFSHNLFFGLCYTIILVKLKCLAIGMADGMVKSGYLTYLYWIQVKILPLKSYPFSFMSLEWTELAVSGTLPPPRCGHTATMVEKRLLVYGGRGEIRGGGPIMGDLWALKGLIEEDTEVPGWTLLKLPGQAPAARCGHTVTSGGHYLLMFGGHGTGGWLSRYDVYYNDCVVLDRGECFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G642</accession>
    <name>A0AAD5G642_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Benzyl alcohol O-benzoyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_003068</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the plant acyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010925">
        <property type="protein sequence ID" value="KAI7729762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G642"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050898">
        <property type="entry name" value="Plant_acyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31147">
        <property type="entry name" value="ACYL TRANSFERASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31147:SF66">
        <property type="entry name" value="OS05G0315700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02458">
        <property type="entry name" value="Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7729762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="454" mass="50392" checksum="A846726212A92794" modified="2024-05-29" version="1">MAQQAPTPLTFTVRRHPTELIVPAKPTPRELKPLSDIDDQAGIRFNITQFHIYPGNPKMGHKNPASVIREALAKALVFYYPFAGRLKEGPGKKLSVDCSGQGVLFIEAEADVTLKEFGVELQPPFPCSEELLYDVPGSNGIVDSPLMLIQVTRLLCGGFIVAIRHNHNMSDVFGYIQFVTALTELARGASTPSTLPVWQRELLCARDPPRVTCAHPEFDELEETDGMIIPFDDMVHRSFFFGPTQISAIRRFVPNHLKKCTTFEVLTACIWRCRTIALQPNPEEDMRLLIIVNARSKFKTPIPVGYYGNVICSPAAISKARDLCNKPLGHALELVKKAKSIVTEEYVRSVADLMATKGRPSSSPGSYIVSDLTRTSLLELDFGWGNAVYAGPDLPVAGFYTRYINHKGEPIIVVPVLLPSYAMKRFAEELDHMLAPTDKDDLNVQKHIGAHSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G7B5</accession>
    <name>A0AAD5G7B5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Cysteine-rich receptor-like protein kinase 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_021631</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010398">
        <property type="protein sequence ID" value="KAI7731740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G7B5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23509">
        <property type="entry name" value="Gnk2-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000336">
        <property type="entry name" value="Cysteine-rich receptor-like protein kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000015">
        <property type="entry name" value="Somatic embryogenesis receptor kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.430.20">
        <property type="entry name" value="Gnk2 domain, C-X8-C-X2-C motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052059">
        <property type="entry name" value="CR_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002902">
        <property type="entry name" value="GNK2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038408">
        <property type="entry name" value="GNK2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47973">
        <property type="entry name" value="CYSTEINE-RICH RECEPTOR-LIKE PROTEIN KINASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01657">
        <property type="entry name" value="Stress-antifung"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51473">
        <property type="entry name" value="GNK2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="237"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="Gnk2-homologous" evidence="13">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="Gnk2-homologous" evidence="13">
        <location>
            <begin position="112"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="291"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="320"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7731740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="578" mass="65596" checksum="FEB1EAE90FB9ADBA" modified="2024-05-29" version="1" fragment="single">MRCGRNAPIGSLTNFIKNRNSTFNELKTQLLNQRLHYARAQDLSGGHSVFAAIQCRNYLSTDQCVACFDECVSELVNCQSGNRGFISFDNCYVRYEDYADFYNDPYVMADSDLTPTLLCGNQSTSQPTVFNQVVDQFLSDIRKATPKTSNFYVASTRQITSENETVYMTAQCVEGMNKGRCQTCMNTAYDTLNDCLPNTQGRFFHLGCFARYSDTPFFNVNQTIDITNLLKGHSSKVAVIAAAVAGAVFLLLALISWLLYRSWKKSKKTEQDFEGAVHYNYKDLQLATNNFSEENILGKGGFGEVFKAVLDDTNVVAVKKLQVKHNGVKDEFENEVKLVSNIHHRNLLRLLGWSVEGSYLLLLVLEYMPNGSLDKFLWGTKRGTLNWTQRYEIVFGIARGLVHLHNEFHIKIVHRDIKSSNILLSDDFKPKIADFGLARFQPEDQSHVSTKFAGTLGYTAPEYALHGVLSEKVDTYSFGIVTLEIISGRRSTEVKSESQSTDYLLEHTWKLYEKKMHVKVIDDTLNLNRYEQEHVMKIIEIALLCTQPVLIRPTMSEVVLMLQEGQSLGKRQLTRPTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G802</accession>
    <name>A0AAD5G802_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_024141</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the fatty acid desaturase type 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010205">
        <property type="protein sequence ID" value="KAI7732535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G802"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016717">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03507">
        <property type="entry name" value="Delta12-FADS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005804">
        <property type="entry name" value="FA_desaturase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021863">
        <property type="entry name" value="FAS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012171">
        <property type="entry name" value="Fatty_acid_desaturase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32100">
        <property type="entry name" value="OMEGA-6 FATTY ACID DESATURASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11960">
        <property type="entry name" value="DUF3474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00487">
        <property type="entry name" value="FA_desaturase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="84"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="176"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="249"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acid desaturase N-terminal" evidence="9">
        <location>
            <begin position="10"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acid desaturase" evidence="8">
        <location>
            <begin position="82"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF11960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7732535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="380" mass="43978" checksum="16194AE0C1D2C442" modified="2024-05-29" version="1">MGSGGRATERNNSSNEVVKRAPSSKPPFTLGDIKKAIPQHCFNRSLIRSSSYLFVDLFFSFLFYYIAATYIIPLQTPLSYVAWPLYWILQGSVQMGLWVIGHECGHQAFSDYPWLNDTIGYFLHTGMLAPYFSWKYSHRRHHSNTASLENDESFVPKKKASLNSFARLLNTPPGRLFRLVILCTIGWLLYVCFNVSGRKYEKFANHFDPKSPIYSDRERFQILLTDVGLLVTSYGLYKVAMAQGFTWLVLVYFAPLVIVYGFLVVITWLHHTHRSLPHYDSTEWNWLRGALSTIDRDYGVLNTVLHHITDTHVAHHLFFTIPHYNAMEATKAIKPILGEYYQFDDTPIIKAMWREATECFFVEAEEGEDNSGVYWFNNKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G9L0</accession>
    <name>A0AAD5G9L0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">MSP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_012532</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the VAMP-associated protein (VAP) (TC 9.B.17) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010213">
        <property type="protein sequence ID" value="KAI7732476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G9L0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090158">
        <property type="term" value="P:endoplasmic reticulum membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061817">
        <property type="term" value="P:endoplasmic reticulum-plasma membrane tethering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000813">
        <property type="entry name" value="Vesicle-associated protein 1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000535">
        <property type="entry name" value="MSP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008962">
        <property type="entry name" value="PapD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016763">
        <property type="entry name" value="VAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10809">
        <property type="entry name" value="VESICLE-ASSOCIATED MEMBRANE PROTEIN-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10809:SF45">
        <property type="entry name" value="VESICLE-ASSOCIATED PROTEIN 2-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00635">
        <property type="entry name" value="Motile_Sperm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019693">
        <property type="entry name" value="VAMP-associated"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49354">
        <property type="entry name" value="PapD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50202">
        <property type="entry name" value="MSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="222"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="MSP" evidence="3">
        <location>
            <begin position="5"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7732476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="243" mass="27489" checksum="B0B52049BF2A32CD" modified="2024-05-29" version="1">MTTELLEIQPQELQIIFELKKQSSCSIRLVNKSDRHVAFKVKTTSSSKYCVRPNTGVIKPITMQAQKEAPPDLICSDKFLIQSTIVPNGTTEDNIMSTTFVKDDGGKCIEEKKLKVILVSPPHSPILSPMNMISRMRPYSTTTGSNDDLVQNNSSHAKVDGVYREVVEAMKNEQSLNRKHTEELRLSNDVEEIKSKLKEFESILRQDNPATNQNARTRETRFQLLFVAVVALFSVYLGYLLHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G9S2</accession>
    <name>A0AAD5G9S2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">TsaA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_027751</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the tRNA methyltransferase O family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009898">
        <property type="protein sequence ID" value="KAI7733842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G9S2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.70">
        <property type="entry name" value="YaeB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2310.10">
        <property type="entry name" value="YaeB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023370">
        <property type="entry name" value="TrmO-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040372">
        <property type="entry name" value="YaeB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036413">
        <property type="entry name" value="YaeB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036414">
        <property type="entry name" value="YaeB_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12818">
        <property type="entry name" value="TRNA (ADENINE(37)-N6)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12818:SF0">
        <property type="entry name" value="TRNA (ADENINE(37)-N6)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01980">
        <property type="entry name" value="TrmO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118196">
        <property type="entry name" value="YaeB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51668">
        <property type="entry name" value="TSAA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="TsaA-like" evidence="3">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00033753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7733842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="187" mass="20984" checksum="52F0844755F5A4AC" modified="2024-05-29" version="1" fragment="single">VRVPRLKGEKMGLFATRSPHRPCPIGLTVAKVESVQESSILLSGVDLVDGTPVLDVKPYLPYCDSIEGAVVPHWVKMDDLLAVASVDFSDDFLATLADCWPAIDKKSLYTSPEEFQILIKQVLSWDIRSVSQRTQVEENYEKTKKEQVHNNEVRDSIYHLNLEGLDIAYRITSDSNVLVENVTISPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GA65</accession>
    <name>A0AAD5GA65_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_017042</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009756">
        <property type="protein sequence ID" value="KAI7734337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GA65"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7734337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="65" mass="7329" checksum="3FEF1432A15EC41B" modified="2024-05-29" version="1" fragment="single">MNGVGGQHANGRLFKTSPNTVLIFINAIGSRMSKTVWSYEDLRFQCLPGQVRTRHLRTSLQVNLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GBC4</accession>
    <name>A0AAD5GBC4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_003402</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="JAMZMK010009784">
        <property type="protein sequence ID" value="KAI7734201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GBC4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF354">
        <property type="entry name" value="SERINE_THREONINE PROTEIN KINASE IRE4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="1"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="21"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7734201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="229" mass="25411" checksum="32F57E939DDDC48C" modified="2024-05-29" version="1">MNSTSDLTRPESNGDLVSNGDHLENELGVDKRERSAVGTPDYLAPEILLGTEHGYAADWWSVGVILFELITGIPPFNSDHPERIFYNILNAKIPWPSVPNEMSYEAQDMINRFLNHDPNQRLGAHGSSEVKAHRFFKGVNWDTLSMQKVAFIPHPNGIDDTSYFVSRHSHSSSATYDDQDCSDAASDTSEFFSDTKENLSQLASINHDVLVQSSKEASRCSSPHNGPTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GC41</accession>
    <name>A0AAD5GC41_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_033569</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010009209">
        <property type="protein sequence ID" value="KAI7736707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GC41"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000476">
        <property type="entry name" value="PAS domain-containing protein tyrosine kinase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF237">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="1"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7736707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="164" mass="18496" checksum="A74544B87A0BB4D2" modified="2024-05-29" version="1">MNYLHNCTPVIVHRDLKSPNLLVDKNWVVKVSDFGLSRMKYSTFLSTRSTAGTAEWMAPEVLRNEPSDEKSDVYSFGVVLWELCTLQQPWGGMNPMQVVGAVGFQHRRLEIPDDMDPVIADTITKCWQTDPKLRPTFADIMAALKPLQKLNTRPIGLHNEAAAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GCA2</accession>
    <name>A0AAD5GCA2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GST C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_028384</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009129">
        <property type="protein sequence ID" value="KAI7737065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GCA2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03185">
        <property type="entry name" value="GST_C_Tau"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045074">
        <property type="entry name" value="GST_C_Tau"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050983">
        <property type="entry name" value="GST_Omega/HSP26"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43968">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43968:SF6">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE OMEGA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST C-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7737065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="118" mass="13597" checksum="FA52D98E10358B97" modified="2024-05-29" version="1">MEGVHPAGRAIWSTKEKDEIEAAKNNFLEIMMYLDGTLGDKDYFNGDTFGLVDILLIGLTSYFPAYEKYGNFKLEDHYPRLAAWITRSWARETVSESLTSPEKITNFVIMIRKMYGLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GFP3</accession>
    <name>A0AAD5GFP3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_013881</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008769">
        <property type="protein sequence ID" value="KAI7738641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GFP3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7738641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="84" mass="9528" checksum="E09A2320A5478F6E" modified="2024-05-29" version="1" fragment="single">LRRRRPPLGLAGVRFAMLVVAKRETETGCFVLLPMTFRPSISSAYVCHDVVIASLFEIWGVRCDDEDIVFDLIVPYGWQTLSLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GFT5</accession>
    <name>A0AAD5GFT5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_033916</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the syntaxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008726">
        <property type="protein sequence ID" value="KAI7738761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GFT5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048278">
        <property type="term" value="P:vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010989">
        <property type="entry name" value="SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045242">
        <property type="entry name" value="Syntaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006011">
        <property type="entry name" value="Syntaxin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957:SF83">
        <property type="entry name" value="SYNTAXIN-16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05739">
        <property type="entry name" value="SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00503">
        <property type="entry name" value="SynN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47661">
        <property type="entry name" value="t-snare proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="231"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="11">
        <location>
            <begin position="192"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7738761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="257" mass="28278" checksum="930ABA1F72680F2C" modified="2024-05-29" version="1">MATRNRTPLFRKYRDALKSVRAPSTFASSSSSSGSGPVIELANAGLFKQNSNYAPLSTEDPGTSSNTGALTVGLPPAWVDVSDEITGNVQRARAKMAELAKAHAKALMPSFGDGREDQHRIEGLTHEITDLLRKSEKKLKKLSAGGTSEDSNIRKNVQRSLATDLQSLSVELRKKQSTYLKRLQQQKEYKFINSTGTIVDRIDHNIQNVAASVDEGLKQLQKAERSQKRGGMIMCATVLVIMCFVMLVLLILKEILF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GG13</accession>
    <name>A0AAD5GG13_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_006936</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008278">
        <property type="protein sequence ID" value="KAI7741057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GG13"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7741057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="314" mass="35580" checksum="9462D2C2EEBBABD9" modified="2024-05-29" version="1" fragment="single">MEPSDIESVLHFLRTNHLSTSESALIDDLFEQSNLNASDIHNFLFPPPPINIPAPHHLVAPSHASSDDDDDDQEFISLASSEFTNPYGIQNLKGASSHASSDRLSQFGTARDYHEFDMQNDLNWYRENDEDYAMPSCFDDADPFGGQSEDKFVMTFEKENQNDDLEVDFDPLLEKTNRLGKVWPTSIRYLEDGVKGIVLDDILDTEKNNKSCDDDDLDEDSKRTHELQNNMCEKDATFGRNGDYDAVKVDEGDDAIDVSLMNGNEDEYEVFDLRIIHRKNRTGFEEHKDLPIVLNSVIGSRYVVSEYLGSAAFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GI38</accession>
    <name>A0AAD5GI38_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GRAM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_024695</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007714">
        <property type="protein sequence ID" value="KAI7743530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GI38"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037848">
        <property type="entry name" value="GEM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31969">
        <property type="entry name" value="GEM-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7743530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="203" mass="22464" checksum="D3789C2204462AE2" modified="2024-05-29" version="1">MSETALNKTSLLYLINNPPVFSLSLLHTNPQILLMNGNKDSVDHRLTTGNMTSSPVHSLFGPSPFLVSSNQNSVFQSPSTENGDLETKRKVTKNHGRNTSNFAYRVRDHVKMGPKLSETLKGKLRLGAKIIQKGGRENIFKEVFGENDGEKLLKASQCYLSTSAGPIAGIFFISTQKLAFCSETSIKLRSANGDLFRNPYKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GIW7</accession>
    <name>A0AAD5GIW7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glycosyltransferase</fullName>
            <ecNumber evidence="5">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_010652</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="5"/>
    <dbReference type="EMBL" id="JAMZMK010007883">
        <property type="protein sequence ID" value="KAI7742839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GIW7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080043">
        <property type="term" value="F:quercetin 3-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080044">
        <property type="term" value="F:quercetin 7-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000019">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926">
        <property type="entry name" value="GLUCOSYL/GLUCURONOSYL TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926:SF870">
        <property type="entry name" value="UDP-GLYCOSYLTRANSFERASE 75B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7742839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="449" mass="49340" checksum="7641C7083390F8E5" modified="2024-05-29" version="1">MSPPQSTTPTTSHRKILIVAYAGQGLINPSLRFANRLLKTGVSVTFCTTFNVMQCIDKQTTPLGLTFAPFSDGHDNHIIGQNPPTTLEQKISDFATHGAPAVAEIITSATAKGEPFAHVVYTTIIPWVARVANAHNVKSTLLWCQPATVLDIYYHYFNGFEELISSNKHNPLFPINLPGLPSLTLADLPSFFFPSSPKEHDFLLQIYKDHVDVIKTGSSVLVNTFVELEVDSIKGSKHFKYLPIGPLNEKDSYGKPADGGYVDWLNTQPKKSVVYVSFGSLSRLSSDQAEEIAAGLLESGRPFLWVIRNPDEAAKLSKISELKKQGLIVSWCCQVEVLSHQAIGCFVMHCGWNSTVEALAAGVPIVAYPQWTDQPTNAKMIEGVWKTGVRVRREGDGVVKGKEIERCVKLVMGDEEISRNVSKWKELANEAINNGGSSTLNLQAFLDDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GIX8</accession>
    <name>A0AAD5GIX8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">Laccase</fullName>
            <ecNumber evidence="4 13">1.10.3.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Benzenediol:oxygen oxidoreductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Diphenol oxidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Urishiol oxidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">M8C21_027882</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">AA19_3_7</strain>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Lignin degradation and detoxification of lignin-derived products.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>4 hydroquinone + O2 = 4 benzosemiquinone + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:11276"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:17594"/>
            <dbReference type="ChEBI" id="CHEBI:17977"/>
            <dbReference type="EC" id="1.10.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
        <text evidence="13">Binds 4 Cu cations per monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Secreted</location>
            <location evidence="2 13">Extracellular space</location>
            <location evidence="2 13">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the multicopper oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.10.3.2" evidence="4 13"/>
    <dbReference type="EMBL" id="JAMZMK010007882">
        <property type="protein sequence ID" value="KAI7742869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GIX8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052716">
        <property type="term" value="F:hydroquinone:oxygen oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046274">
        <property type="term" value="P:lignin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13849">
        <property type="entry name" value="CuRO_1_LCC_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13875">
        <property type="entry name" value="CuRO_2_LCC_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13897">
        <property type="entry name" value="CuRO_3_LCC_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000062">
        <property type="entry name" value="Laccase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001117">
        <property type="entry name" value="Cu-oxidase_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034288">
        <property type="entry name" value="CuRO_1_LCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034285">
        <property type="entry name" value="CuRO_2_LCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034289">
        <property type="entry name" value="CuRO_3_LCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017761">
        <property type="entry name" value="Laccase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03389">
        <property type="entry name" value="laccase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF446">
        <property type="entry name" value="LACCASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00394">
        <property type="entry name" value="Cu-oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13" id="KW-0052">Apoplast</keyword>
    <keyword evidence="10 13" id="KW-0186">Copper</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 13" id="KW-0439">Lignin degradation</keyword>
    <keyword evidence="7 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 13" id="KW-0964">Secreted</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041776894" description="Laccase" evidence="13">
        <location>
            <begin position="22"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="16">
        <location>
            <begin position="30"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="14">
        <location>
            <begin position="160"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="15">
        <location>
            <begin position="399"/>
            <end position="538"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAI7742869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="556" mass="62165" checksum="D147EE1EFD84EAC7" modified="2024-05-29" version="1">MTITWGLFLSLLFCIAFTTFAETHHHEFEVQEIEARRLCRNHRIVAVNGQFPGPTLEVRNGDSVVVKVTNVAPYNVTIHWYFMHGLRQLRNPWADGPEYVTQCPIRPGGSYTYRFTITDQEGTLWWHAHSQWLRATVYGALVIRPKLGSSYPFPKPKLEFPVILGEWWDRKVISVQRQALFSGAAPNISDAITINGQPGDLFRCSSQGDRVLLRVINAALNQQLFFSVANHKLIVVATDALYTKQFTTNVIMLGPGQTTDVLLTADQQPGRYYMAARAYESARNAPFDNTTATAILEYKSTTSQPILPQLPAYNDTNTVTAFSNQIKSLGKVTVPTKIDENLFITVGLGFFNCTPGPRCQGPNNTRFGASMNNVSFVLPRRVSLLQAYTQKIPNIYTTDFPPVPPVKFDYTGNVPRGLWQPVKGTKLYNLKFGSSVQIVLQDTSIFSTDDHPVHLHGYHFYVVGQGFDNFNPSRDTANFNLVDPPQRNTIDVPVGGWAAIRFIADNPGTTCETGVWFMHCHIDAHLAWGFGMAFIVENGVGEAETLLPPPFDLPQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GJA3</accession>
    <name>A0AAD5GJA3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_003256</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the chloride channel (TC 2.A.49) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008133">
        <property type="protein sequence ID" value="KAI7741823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GJA3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009705">
        <property type="term" value="C:plant-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009671">
        <property type="term" value="F:nitrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005247">
        <property type="term" value="F:voltage-gated chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04591">
        <property type="entry name" value="CBS_pair_voltage-gated_CLC_euk_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03685">
        <property type="entry name" value="ClC_6_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3080.10:FF:000004">
        <property type="entry name" value="Chloride channel ClC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3080.10">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051280">
        <property type="entry name" value="Cl-channel/antiporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014743">
        <property type="entry name" value="Cl-channel_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002251">
        <property type="entry name" value="Cl_channel_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001807">
        <property type="entry name" value="ClC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11689">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN CLC FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11689:SF67">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN CLC-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00654">
        <property type="entry name" value="Voltage_CLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00762">
        <property type="entry name" value="CLCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01120">
        <property type="entry name" value="CLCHANNELPLT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81340">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0129">CBS domain</keyword>
    <keyword evidence="11" id="KW-0868">Chloride</keyword>
    <keyword evidence="10" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="6" id="KW-0407">Ion channel</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="90"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="132"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="282"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="334"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="374"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="460"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="543"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="769"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7741823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="769" mass="85086" checksum="1F585334DC2322FF" modified="2024-05-29" version="1" fragment="single">MDKDSDKLAHTATNMEQQTNQQEKDPETNTLCQPLLKKNQMLSSSPLALVGTKVSLNESLDYEINENDLFKHDWRTRSQTQVLQYVFVKWLLAFLVGLLTGLITTVINLSIENIAGYKLLAVVNYIERKRYMMGFFFMTAVNFLLTLVATLLCVFFAPTAAGPGIPEIKAYLNGVDTPDMYGATTMFVKIIGSIGAVSAGLDLGKEGPLVHIGACIASLLGQGGPDNHRIQWKWIRYFNNDRDRRDIITCGASSGVCAAFRSPVGGVLFALEEVATWWRSALLWRTFFSTAVVVVVLRAFMEYCKSGECGLFGEGGLIMFDVSGVPVKYHIVDLLPVTVIGIIGGVLGSLYNYLLHKVLRLYNIINAKGKLAKILLSLTVSLFTSVCLYGLPFLASCTPCNRSAADSECPTTGRMGNFKKFNCPKGHYNDLATLLLTTSDDAVRNIFSINTPGEYSVSSLLIFFALYCILGLFTFGIAVPSGLFLPIILMGSAYGRLLGMAMGPYTTIDQGLYAVLGAASLMAGSMRMTVSLCVIFLELTNNLLLLPITMLVLLISKTVGDCFNPSIYEIILDLKGLPFLEGRPEPWMRNISVGELADIKPPLVTLSGIEKVGRIVDVLRNTTHNAFAVVDNTLVPSVGQMSEVHGLVLRTHLLLVLKKKWFLKERRRTDEWEVREQFTWVDVAERWGTIEEVAVTKDEMEMYVDLHPLTNTTPYTVVETMSVAKALVQFREVGLRHMLVPPVVGILTRQDLRAHNILIKEGTTRMETS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GLA2</accession>
    <name>A0AAD5GLA2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein LURP-one-related 15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_017789</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LOR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007362">
        <property type="protein sequence ID" value="KAI7745089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GLA2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.200">
        <property type="entry name" value="LURP1-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007612">
        <property type="entry name" value="LOR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038595">
        <property type="entry name" value="LOR_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025659">
        <property type="entry name" value="Tubby-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31087">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31087:SF58">
        <property type="entry name" value="OS07G0230700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04525">
        <property type="entry name" value="LOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54518">
        <property type="entry name" value="Tubby C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7745089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="213" mass="23883" checksum="F8227DBAA7673E66" modified="2024-05-29" version="1">MGFAFEVSSGSPQQLSKPICVVSHQFVSPNPIHMLSGKKLLALSDGNFGVTDVNGNLMFKLKGKLLSVRGRRLLVDGAGNRILTLHKKLTSARKRWQCYRGDSTDPRDHVFSAKQVNRTQFKTSLDVFLASNRTQDVADFTVKGNWFEKRCTIYVGESTTIIAEINKKQTNQLMAFGVDNYTITVYPNVDYVFVVALTAILNEINDEKDDDEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GQS0</accession>
    <name>A0AAD5GQS0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">SBP-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_028211</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Probable transcriptional factor. Binds to the promoter of the SQUAMOSA gene.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006396">
        <property type="protein sequence ID" value="KAI7749156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GQS0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1100.10:FF:000001">
        <property type="entry name" value="Squamosa promoter-binding-like protein 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1100.10">
        <property type="entry name" value="Transcription factor, SBP-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044817">
        <property type="entry name" value="SBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004333">
        <property type="entry name" value="SBP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036893">
        <property type="entry name" value="SBP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31251:SF74">
        <property type="entry name" value="SQUAMOSA PROMOTER-BINDING-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31251">
        <property type="entry name" value="SQUAMOSA PROMOTER-BINDING-LIKE PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03110">
        <property type="entry name" value="SBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103612">
        <property type="entry name" value="SBT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51141">
        <property type="entry name" value="ZF_SBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SBP-type" evidence="12">
        <location>
            <begin position="144"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="205"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="205"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00056472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7749156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="362" mass="40311" checksum="B899D3BE87E67B1F" modified="2024-05-29" version="1">MEWNTKFDWENLDIDGSSFNLPDVIGGSGGSVSDIGNNSSTKSSISASTESSFKDGIKRSNSSFEGFGCFSDCYNKDELNGIGQLDGSVCSGEPFIGLKLGKRTYFENSYPKNNKTSSVLDVPVSPVVSTGKKVKSSSGQSTPVSRCQVEGCNLDLSSAKEYHRKHKVCESHSKSPKVIVAGLERRFCQQCSRFQSMSEFDEDKRSCRKRLSDHNARRRKPQYEPSRYNSTDPYSPFYTTESMDVMTHSRGVQLSEALNTSTFACNRLVPSKGTRAEIFDQDAVDIRRALSLLSNNTWGSFELDHSTHATGPTMVQHGLHTVHQGSHLNSQDYWQANQQPTDPHIQIMKSSYENSFQFQHNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GRG5</accession>
    <name>A0AAD5GRG5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Disease resistance protein Roq1-like winged-helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_026985</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005694">
        <property type="protein sequence ID" value="KAI7752410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GRG5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058192">
        <property type="entry name" value="WHD_ROQ1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11017">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11017:SF492">
        <property type="entry name" value="TIR DOMAIN, P-LOOP CONTAINING NUCLEOSIDE TRIPHOSPHATE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23282">
        <property type="entry name" value="WHD_ROQ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance protein Roq1-like winged-helix" evidence="2">
        <location>
            <begin position="27"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7752410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="142" mass="16244" checksum="E614AF6EB5E56536" modified="2024-05-29" version="1">MSSKGDLDYNIQGVLQKSFDCLPLCSHRELFLHIACFFVGEYKNVMEMILEDELYAKSGISTLCHRCLLTISADGKLMMHQLLQEMGRRIVCEESKDPTKRSRVWHDAESYHVLRKGDGSDTIEALALDMRNVKQMTGSEVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GRL8</accession>
    <name>A0AAD5GRL8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">dihydrolipoyllysine-residue succinyltransferase</fullName>
            <ecNumber evidence="6">2.3.1.61</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">M8C21_030731</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">AA19_3_7</strain>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>N(6)-[(R)-dihydrolipoyl]-L-lysyl-[protein] + succinyl-CoA = N(6)-[(R)-S(8)-succinyldihydrolipoyl]-L-lysyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:15213"/>
            <dbReference type="Rhea" id="RHEA-COMP:10475"/>
            <dbReference type="Rhea" id="RHEA-COMP:20092"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:83100"/>
            <dbReference type="ChEBI" id="CHEBI:83120"/>
            <dbReference type="EC" id="2.3.1.61"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>(R)-lipoate</name>
            <dbReference type="ChEBI" id="CHEBI:83088"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid degradation; L-lysine degradation via saccharopine pathway; glutaryl-CoA from L-lysine: step 6/6.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Forms a 24-polypeptide structural core with octahedral symmetry.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the 2-oxoacid dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.61" evidence="6"/>
    <dbReference type="EMBL" id="JAMZMK010006085">
        <property type="protein sequence ID" value="KAI7750789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GRL8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045252">
        <property type="term" value="C:oxoglutarate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004149">
        <property type="term" value="F:dihydrolipoyllysine-residue succinyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06849">
        <property type="entry name" value="lipoyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.10:FF:000006">
        <property type="entry name" value="Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003016">
        <property type="entry name" value="2-oxoA_DH_lipoyl-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050537">
        <property type="entry name" value="2-oxoacid_dehydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001078">
        <property type="entry name" value="2-oxoacid_DH_actylTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006255">
        <property type="entry name" value="SucB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01347">
        <property type="entry name" value="sucB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43416:SF44">
        <property type="entry name" value="DIHYDROLIPOYLLYSINE-RESIDUE SUCCINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43416">
        <property type="entry name" value="DIHYDROLIPOYLLYSINE-RESIDUE SUCCINYLTRANSFERASE COMPONENT OF 2-OXOGLUTARATE DEHYDROGENASE COMPLEX, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00198">
        <property type="entry name" value="2-oxoacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00189">
        <property type="entry name" value="LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9" id="KW-0450">Lipoyl</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="7" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="16">
        <location>
            <begin position="13"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="89"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="15">
        <location>
            <begin position="116"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="128"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00037426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00052761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAI7750789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="386" mass="41823" checksum="D0DC44BE479B2514" modified="2024-05-29" version="1">MWSRSFSSDSGDLVDAVVPFMGESISDGTLAVFLKKPGDRVEIDEPIAQIETDKVTIDVASPEAGVIQEFVAKEGDTVEPGTKVAIISKSGEGVTTHVAPSEPPTQEAAAKVEKPPSSPPPPPPPPQSEIQPPKAAPKQPSPKPSATEPQLPPKDRERRVPMTRLRKRVATRLKDSQNTFALLTTFNEVDMTNLMKLRSEYKDAFLEKHGVKLGLMSGFVKAAVSGLQNQPIINAVIDGDDIIYRDYIDISIAVGTPKGLVVPVIRDAEKMNFAEIEKTINTLAKKANSGSLSIDEMAGGSFTISNGGVYGSLLSTPIINPPQSAILGMHSIVNKPMVVGGEIQARPMMYVALTYDHRLIDGREAVFFLRRIKDVVEDPRRLLLDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GRU0</accession>
    <name>A0AAD5GRU0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Auxin-responsive protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_026303</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Aux/IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimers and heterodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Aux/IAA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006189">
        <property type="protein sequence ID" value="KAI7750206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GRU0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009734">
        <property type="term" value="P:auxin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000078">
        <property type="entry name" value="Auxin-responsive protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033389">
        <property type="entry name" value="AUX/IAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003311">
        <property type="entry name" value="AUX_IAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053793">
        <property type="entry name" value="PB1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31734:SF265">
        <property type="entry name" value="AUXIN-RESPONSIVE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31734">
        <property type="entry name" value="AUXIN-RESPONSIVE PROTEIN IAA17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02309">
        <property type="entry name" value="AUX_IAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51745">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0927">Auxin signaling pathway</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0678">Repressor</keyword>
    <keyword evidence="5 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 8" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="PB1" evidence="9">
        <location>
            <begin position="201"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7750206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="318" mass="34844" checksum="1E83F8C5519DD977" modified="2024-05-29" version="1">MTMSSSVDGEEEGQRKVNLLTSSPSIDGVCDIRERNYMGLSDCSSVGSSALSPSSEDVNLSLNLKATELRLGLPGSRSPERDHELCLLMSPRRDEKPLFPLFSPKSVGSGSKRGFADTVDSFIEGTLRSNPEPLSIQESSCVANKANQNQTNAASNINTAPTTKTQVVGWPPIRSSFRKNSVATITSKKSNEVERKTRDRALFVKVSMDGAPYLRKVDLSAYSVYQELSSALEKMFGCFTIGQYGSHGNEKLSETKLKDLLHGSEYVLTYEDKDGDWMLVGDVPWEMFINTCKRLRIMKSCDAIGLAPRAVDLSCNGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-06-18" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GT94</accession>
    <name>A0AAD5GT94_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Sucrose-phosphate synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_026206</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005862">
        <property type="protein sequence ID" value="KAI7751646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16419">
        <property type="entry name" value="HAD_SPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1070.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006380">
        <property type="entry name" value="SPP-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044161">
        <property type="entry name" value="SPS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035659">
        <property type="entry name" value="SPS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000368">
        <property type="entry name" value="Sucrose_synth_GT-B1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46039:SF10">
        <property type="entry name" value="SUCROSE-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46039">
        <property type="entry name" value="SUCROSE-PHOSPHATE SYNTHASE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00862">
        <property type="entry name" value="GT-B_Sucrose_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05116">
        <property type="entry name" value="S6PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sucrose synthase first GT-B" evidence="4">
        <location>
            <begin position="167"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="domain" description="Sucrose phosphatase-like" evidence="5">
        <location>
            <begin position="444"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="101"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="101"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="123"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF05116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7751646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="664" mass="75136" checksum="C57D2939CE004280" modified="2024-05-29" version="1">MAGNDWINSYLEAILDVGPGLDDAKSSLLLRERGRFSPTRYFVDNVIGFDETDLHRSWVKAQATRSPQERNTRLENMCWRIWNLARQKKQLESEEAQRMTRRRLERERGRREAVADMSEDLSEGEKGDIVSDVSAHGDNHKSRLPRISSTDAMEAWANQQKGKKLYLVLISLHGLIRGENMELGRDSDTGGQVKYVVELARALASMPGVYRVDLLTRQVASPEVDWSYGEPTEMLAPHNSDSLTDEIGESSGAYIIRIPFGPRDKYIPKELLWPHVPEFVDGALSHIIQMSKVLGEQVGGGRPVWPVAIHGHYADAGDSAALLSGALNVPMLFTGHSLGRDKLEQLLRQGRLSKDEINKTYKIMRRIEAEELTLDASEVVITSTRQEIEEQWRLYNGFDPVLERKLRARIRRNVSCYGRFMPRMVMAEIHSYLVSNGLSPSDFDAFICNSGADLYYSSXXXXPFVFDLYYHSHIEYRWGGEGLRKTLVRWASSIIDKKAQNKNEEHVVTEDESVSTNYCYAFNVRNPALVPPAKELRKMMRIQALRCHIIYCQNGRKINVIPVLASRSQALRYLYLRWGMDLSKVVVFIGESGDTDYEGFLGGVHKTVILKGVSNTISDQLHANRIYPLSDVWPTDSANVIQTPEQFGVADIRGSLAKLGVLKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GTG2</accession>
    <name>A0AAD5GTG2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_013449</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010003049">
        <property type="protein sequence ID" value="KAI7754555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GTG2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058192">
        <property type="entry name" value="WHD_ROQ1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11017">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11017:SF492">
        <property type="entry name" value="TIR DOMAIN, P-LOOP CONTAINING NUCLEOSIDE TRIPHOSPHATE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23282">
        <property type="entry name" value="WHD_ROQ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NB-ARC" evidence="3">
        <location>
            <begin position="100"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein Roq1-like winged-helix" evidence="4">
        <location>
            <begin position="350"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7754555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="468" mass="53152" checksum="50C7886906062214" modified="2024-05-29" version="1" fragment="single">ATHRGTFKWDLKIKTYVPTPTERNQRGSYSIEAKEGLKWTEDNVNRWKAALREVADLKGMVLSGYEKEFIANIVQKVYQQLGLECLTSPIDLTGIEARAEVINSWLRYEQPDFPVLAICGMGGGGKTTLAKHVYYSNKHNFDSCSLLENIENQPDRLVDVQRQLLKDISGNNNIMISNVDEGAFQLEMAIRMNKVLIVVDDIDDKDTLSKLFGTKALHTQSKIIITTRIQNIDGWYVSISGGCRVHKIELLNELESLELLSFHAFGSKFPMEGFEELALQLAQYCGGNPLALKVLGSSLSVRAQDQWHINNMIEIWRSRMNSLNSSNGDLDCKIKVVLKRSYESLADLHREFFLLIACFFVEEYKGKVEMILENDYQAKFGIMTLINRCLVTVTVERGSPRLVMHKLLQNMARDIVREESKDPAKRSRVWQSDESYHLLSKGDGSETIEGLYLNIAQAKQGMKSAGYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GTV0</accession>
    <name>A0AAD5GTV0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Bms1-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_003200</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TRAFAC class translation factor GTPase superfamily. Bms1-like GTPase family. TSR1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010002043">
        <property type="protein sequence ID" value="KAI7755035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GTV0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034511">
        <property type="term" value="F:U3 snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000479">
        <property type="term" value="P:endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012948">
        <property type="entry name" value="AARP2CN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039761">
        <property type="entry name" value="Bms1/Tsr1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007034">
        <property type="entry name" value="BMS1_TSR1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030387">
        <property type="entry name" value="G_Bms1/Tsr1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12858:SF1">
        <property type="entry name" value="PRE-RRNA-PROCESSING PROTEIN TSR1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12858">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08142">
        <property type="entry name" value="AARP2CN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04950">
        <property type="entry name" value="RIBIOP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22298">
        <property type="entry name" value="Tsr1_G-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00785">
        <property type="entry name" value="AARP2CN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01362">
        <property type="entry name" value="DUF663"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51714">
        <property type="entry name" value="G_BMS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Bms1-type G" evidence="6">
        <location>
            <begin position="79"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="393"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="11"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="29"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="396"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7755035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="795" mass="89479" checksum="E22F9508E72E0235" modified="2024-05-29" version="1">MAGGSRSQVNKSHKTRFASKSSRNIHKTSVKDKSKISKPDRNVVKGARAARLQRNKMMREQKRATLLKEKRASSATTSAPRVIVLFGLSASTNVSSLAGDLVALLSNENTGIQFPAVASSEYRLRATVLQAPHGDLMACMEMAKMADLIVFVASANCSSERGSSSYYIDDFGSQCLSVFRSLGLPSTAVFLRDLPSDLKKRNDLKKTCISSLSSEFPEDCKFYPADTKDDLHKFMFLFKEQRLTIPHWRTQRPYLMGHKVDMVAEESGKCTLRITGYLRARNLSVNQLVHVSGAGDFQLGKIELLKDPCSLNVRKEGDFMDADEVNDLQAIKTLLPDPMKQEPLLVENIPDPLQGEQTWPTEAEMEEADRLHKEKKHKKRSLPRGTSEYQAAWIVDDSDVSGTDSDDDGSENGMVVDEGEHDFTGQGDGGKFDHEFDQASLNLRDSDEETEADSMMMDNDNLTKEQIEEDIRKIKEAHAEDEEFPDEVDAPVDIPARKRFAKYRGLKSFRTSSWDPKESLPPEYARIFAFDNFTRTHKHVLAKARDMEQGNSDECVLANSYVALHISQVPVHIASKLCTQGKTMPVIACGLLQHESKISVLHFRLKKHETYTEPIKAKEELIFHVGFRQFITRPTFASDNFNSDKHKMERFLHAGRFSIASVYAPISFPPLPLIVLKSRGDSLAPALAAVGSLKSIDPDKIILKKIILTGYPQRVSKSKAAVRYMFHSPEDVRWFKPVEVWTKLGRRGRIKEPVGTHGAMKCLLNGVLQQNDTVCMSLFKRSYPKWPEHWFPMST</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GTV6</accession>
    <name>A0AAD5GTV6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sulfotransferase</fullName>
            <ecNumber evidence="3">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_006532</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the sulfotransferase 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010001845">
        <property type="protein sequence ID" value="KAI7755065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GTV6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11783">
        <property type="entry name" value="SULFOTRANSFERASE SULT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sulfotransferase" evidence="4">
        <location>
            <begin position="63"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7755065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="165" mass="19073" checksum="A053DB4BEA15BD93" modified="2024-05-29" version="1" fragment="single">QSSMETSTKTQLRSIDEMVKTLPQHSLSWLKGKLTLYNYQGIWIHRKFLEGLPLAQQSFKPQPSDVFLCSHPKSGTTWLKAVVFAIMTREKFDEFNTPLHTTMPHDCIPFLSRDIEHILENRHNNSSCITPIATHLPYNLLPESIRASNCKIVYMYRNVKDVISL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GUR8</accession>
    <name>A0AAD5GUR8_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_010963</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000416">
        <property type="protein sequence ID" value="KAI7756387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GUR8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35510">
        <property type="entry name" value="DBH-LIKE MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35510:SF1">
        <property type="entry name" value="DBH-LIKE MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7756387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="192" mass="21277" checksum="4EEED0727DAF6870" modified="2024-05-29" version="1">MEEEDVSNPVAFGQLEPSQAIEDTSGILPVNDERAIVLYDPTNNTPLLQTRSPFTISVNSNFLSGFKNLVFWSNRSHALDSSNTIKEKQENCYDQSNGSLAVVPWVPMHSQHQTMAEVEIPHTEEVSEMMDADDTEPITMDVEDDDNSQRVHINEPGSVSGVEGVNQWQQHWMIPQSAHSLSAPVAWSGTGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GW94</accession>
    <name>A0AAD5GW94_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_015919</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010001117">
        <property type="protein sequence ID" value="KAI7755454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GW94"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="95"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="105"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7755454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="115" mass="12947" checksum="DD37BDDBC46E3EE7" modified="2024-05-29" version="1" fragment="single">VILGISMQRTRVGLTHLWRLPNNGNNNNERTVSPIWPKIVAVQHGNNGSSSKTMTKGLKATMVDLDIDSDDEIGIRERRYVTFGRRKKEFCGVWCSSTGSTNERTSNDSKYTTED</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GWW7</accession>
    <name>A0AAD5GWW7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Expansin-like EG45 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_011178</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000635">
        <property type="protein sequence ID" value="KAI7756024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GWW7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009627">
        <property type="term" value="P:systemic acquired resistance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22269">
        <property type="entry name" value="DPBB_EG45-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.10:FF:000005">
        <property type="entry name" value="Barwin-related endoglucanase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="entry name" value="RlpA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044206">
        <property type="entry name" value="EGC1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007112">
        <property type="entry name" value="Expansin/allergen_DPBB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009009">
        <property type="entry name" value="RlpA-like_DPBB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47295">
        <property type="entry name" value="EG45-LIKE DOMAIN CONTAINING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47295:SF11">
        <property type="entry name" value="RLPA-LIKE DOUBLE-PSI BETA-BARREL DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03330">
        <property type="entry name" value="DPBB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00837">
        <property type="entry name" value="DPBB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="Barwin-like endoglucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50842">
        <property type="entry name" value="EXPANSIN_EG45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042132197" description="Expansin-like EG45 domain-containing protein" evidence="4">
        <location>
            <begin position="23"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="Expansin-like EG45" evidence="5">
        <location>
            <begin position="21"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7756024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="148" mass="15819" checksum="5606223CCE377610" modified="2024-05-29" version="1">MASIRIYASILVVMSLLAFGSSESGIGTINDPPYLPSACYGFQDQGVMIAAANQDLWQGGAACGKNFQVTCTGATNQGVPHPCTDTPTVTVMITDFCPPPGCKGDLDLSHEAFSTIADPRAGGIKISYQEYGVKKKYHRGCFRFKEDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5H0J6</accession>
    <name>A0AAD5H0J6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_018755</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000016">
        <property type="protein sequence ID" value="KAI7758233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5H0J6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7758233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="96" mass="10429" checksum="8555ED8458BA461E" modified="2024-05-29" version="1" fragment="single">VQFGSSNFQPPDLFFQGPAALNVSRRRSPVQSVNAEPKTNDSIVPCAATVFADDVVDKAVKVEDIEKLALDLQKASPLEIIEEGNLATGRVEEELF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BKG4</accession>
    <name>A0AAD5BKG4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_031226</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012160">
        <property type="protein sequence ID" value="KAI7724801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BKG4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48463">
        <property type="entry name" value="DUF223 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48463:SF1">
        <property type="entry name" value="DUF223 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="359"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="359"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="394"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7724801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="403" mass="45333" checksum="ED48B5AE4DB615AE" modified="2024-05-29" version="1" fragment="single">SIVPDSDSAPIAVRLIRKWLRNNNLCYFIIDKHGDGIQADVHYLDKDFFEKKVTLLSCYILDNYICTEAPASIRVIAHPASIHLGTTTSVGLIPTPETFPLHYFNFCPYADLPLRTEENHPANQPYILTDYVGRLEDTTPSNTKKMRPYLRVTLTDISGPTIDVALWEDVLPRFDRQAMLDAEEPIIVAFTSLKVSIFKGYVVEKLQLSSTPATHLYFNPSAESATVIRNRFKAGSICCHQLLDHRPNTYVWKTGTEGQFQTFCNTMVKATRYCIGAEIKDNTAPTTATFFDDAAATLLGIQCKDMVLQYGLDRYTLPKQMHDVIEHDMIMQLQYARSIGNTSNVFSINKVFVSPPTITATPPSPSQQTTSAIARLPPTKPTAKRQLYQPEGNSDPKRLRFTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BN42</accession>
    <name>A0AAD5BN42_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_029681</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011975">
        <property type="protein sequence ID" value="KAI7725333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BN42"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050665">
        <property type="entry name" value="Cytochrome_P450_Monooxygen"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24282">
        <property type="entry name" value="CYTOCHROME P450 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24282:SF266">
        <property type="entry name" value="CYTOCHROME P450-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11 12" id="KW-0349">Heme</keyword>
    <keyword evidence="8 11 12" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 12" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="7 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="binding site" description="axial binding residue" evidence="11">
        <location>
            <position position="429"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7725333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="481" mass="54598" checksum="2F92D8B4399C027E" modified="2024-05-29" version="1">MRIKYVMSSQGISGPPYTFIYGNTKDISNMINKSTSSPMDISHYIFPRIQPHFHSWLHIYGKNILYWIGPQAELVVTEPELLKEIMSAREISMGKPDVGPIFSKILGGGLVLSQGDKWAKQRKIATHAFNGQRLKNMVPAMVESAKMMLERWQVAGTKEMEVYNEFKILASDVISRTAFGSSYEEGKQLFQKLWALTMLVSKTANKTRLPGIGKIFKDKDVVESEKLQSGVHDLIMQMIRNKEKTIILGDEDNSATDYLELLLKARYDIDDDYKLSIHDIIDDCKTFYSAGHATISLLLSWVTFLLGINTKWQEKAREEVQEVFGNQNPSLEGITRLKKMGMIINETLRLYPPSISTNRKVMKETRVGNMLLPANINVHIPALAFHQDPEIWGEDAHQFKLERFSEGIANAINNNPGAFLPFGYGPRICVASNFAINEAKIVLSMILQRYRFTISPNYVHAPVQNISLRPQYGVKIMFQAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BNQ4</accession>
    <name>A0AAD5BNQ4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-CH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_028279</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011538">
        <property type="protein sequence ID" value="KAI7726766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BNQ4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16495">
        <property type="entry name" value="RING_CH-C4HC3_MARCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000146">
        <property type="entry name" value="RING/FYVE/PHD zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022143">
        <property type="entry name" value="DUF3675"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033275">
        <property type="entry name" value="MARCH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23012">
        <property type="entry name" value="RING/FYVE/PHD ZINC FINGER DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23012:SF175">
        <property type="entry name" value="RING_FYVE_PHD ZINC FINGER SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12428">
        <property type="entry name" value="DUF3675"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12906">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51292">
        <property type="entry name" value="ZF_RING_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="136"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="175"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="RING-CH-type" evidence="6">
        <location>
            <begin position="29"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="14"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7726766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="256" mass="28055" checksum="A3C0F43451F41373" modified="2024-05-29" version="1">MGDHLVLEVNRPIQRSEERAARSSSDEKGPLLSVAECRICQEEDSVDALETPCACNGSLKYAHRKCVQHWCDEKGNITCEICHQPYQPGYVAPSPRPCLEETTIDIGGGWQMSEADRQYLEAEYGAYNATNTSGAAFLRSAVLIMMALLLLRHASSVPNSDGGDGDEDTTTFLTLFLLRIAGFLLPCYIMIWAITILQRRRQRQEALAASQIAFVLQAGERRRLHLTVASLGLTPAHATAPAPTVTPTAAPQDENV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BNW0</accession>
    <name>A0AAD5BNW0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Transcription factor MYB59-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_002161</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Transcription factor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011535">
        <property type="protein sequence ID" value="KAI7726785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BNW0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000011">
        <property type="entry name" value="Myb transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000259">
        <property type="entry name" value="MYB transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044676">
        <property type="entry name" value="EOBI/EOBII-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45675">
        <property type="entry name" value="MYB TRANSCRIPTION FACTOR-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45675:SF126">
        <property type="entry name" value="MYB-RELATED PROTEIN MYBAS1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="10">
        <location>
            <begin position="18"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="9">
        <location>
            <begin position="18"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="10">
        <location>
            <begin position="71"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="9">
        <location>
            <begin position="71"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="127"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="133"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00057804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7726785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="220" mass="25788" checksum="C207D4FF196E99AB" modified="2024-05-29" version="1" fragment="single">LQKQDRKMGQDDDMMMMMMMMRKGPWTEQEDVQLVSFVNMCGDRRWDFIAKVSGLKRSGKSCRLRWVNYLHPGLKRGKMSQQEERRILELHTKWGNRWSKIARKLPGRTDNEIKNYWRTHMRKKAQEKKRALSSSPSMSSSSIYSSITSNPTVDTKLVAETKERSFYDTGGLEMVTITNFECDNYKMKSTDTSVNSTFEQDGYSSMDEIWKDITLLDDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BPH7</accession>
    <name>A0AAD5BPH7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein LONGIFOLIA 1-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_028833</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011750">
        <property type="protein sequence ID" value="KAI7726111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BPH7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051513">
        <property type="term" value="P:regulation of monopolar cell growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032795">
        <property type="entry name" value="DUF3741-assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025486">
        <property type="entry name" value="DUF4378"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033334">
        <property type="entry name" value="LNG1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31680">
        <property type="entry name" value="LONGIFOLIA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31680:SF12">
        <property type="entry name" value="OS11G0587300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14309">
        <property type="entry name" value="DUF4378"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14383">
        <property type="entry name" value="VARLMGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF3741" evidence="3">
        <location>
            <begin position="158"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4378" evidence="2">
        <location>
            <begin position="573"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="172"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="219"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="334"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="183"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="219"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="334"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="346"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="370"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="457"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7726111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="733" mass="82483" checksum="A6AB6ADA941699E6" modified="2024-05-29" version="1">MQDQIEKQIGCMSGFLHIFDRQHIFAEKRIHSAKCLSTSAGVAVFPDSVKSVNSVKSTAIREIEKPKLVETVVSSSTSESGGGFSQSPTANLKFFPTSTPPRSPLPLPLFEVKNSWKFYKEMPRLSLDSRAIVDSKGSLCPKEIRPDSLNGSVDNHGRSPSVIAKLMGLEQLPSSVSSPSPEHVTKPELRRSASESRVSTNLFHSGFIKGNNLQTKRQIQSHKQSSATTDVSNISEIKYSSRSQNNKPSPLRRYPIPSQRRNFFDSSDIFPEPKHKRMLLSGKNYEQSKDLEALKNILEALQLKGLLHSINKDSHHNFVYDHSNIVHMNRHHSCSSNRRYSNDISPKTDRGVFDRGSRSPVRARKSAVRSKTELNPKNRNANDKRNPLCVETQKRVRECSDSRRNSPISSPKLTPKRNGHEHGSVVSSRSPRSRRPSDKSCLKQKVCRTVVVEDESSSVSESSGSTTSQFDVERSKMEEYGQGKSLLERCDKLLAEMNNIAESHTSSNSVLPSPVSVLNSSFDKDESLSPSPVKKRTIDFKDLTNDPDDDLWSTIISPATPTEVEGFISDDSDFKYISQILQASKHLPIDSTAFFFIEKQQYPIENNPSNGSKLQRKLVFDIVTEILNRNKDLPPWNTTSLTKSSSCVKNICSEFQKIRETQTAENVLDLICFMLKKDLAEDNRWGDHPLEKSEAVLYIERLIFKDLVSEMIRNLAEFAGKPTFLAPRRKLVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BPM2</accession>
    <name>A0AAD5BPM2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box/LRR-repeat protein 15-like leucin rich repeat domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_033243</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011476">
        <property type="protein sequence ID" value="KAI7726944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BPM2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057207">
        <property type="entry name" value="FBXL15_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25372">
        <property type="entry name" value="DUF7885"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box/LRR-repeat protein 15-like leucin rich repeat" evidence="1">
        <location>
            <begin position="68"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF25372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7726944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="164" mass="18458" checksum="627C1D7A5F5F8F2B" modified="2024-05-29" version="1">MAEPSNRTHKFNNEMSLDRLSSEVLATIISKLDVSSICAVTLTSKTFHISTQDIFHFLPNFHLLEIAAPIDRLRRLLVPNASLRSLKLDCRRLNESSIDCILQPGLVELTLRNCYKFSGKLLSEVGARCKHLRSLYVSSVADNRGHIHDVCDLEELLRGTCPNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BQ83</accession>
    <name>A0AAD5BQ83_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7798 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_032097</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011634">
        <property type="protein sequence ID" value="KAI7726546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BQ83"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056700">
        <property type="entry name" value="DUF7798"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36011">
        <property type="entry name" value="BAT2 DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36011:SF1">
        <property type="entry name" value="BAT2 DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25074">
        <property type="entry name" value="DUF7798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7798" evidence="2">
        <location>
            <begin position="248"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="22"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7726546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="515" mass="55363" checksum="CA3C959E56C1D354" modified="2024-05-29" version="1" fragment="single">IKPSSNDDEIQPAQDNHPKQHTTTTTTNNNTTTAAAATGSGGGGWGWGFSPLSVLSDLQKAAEEISRNAAEVAKTAANSISDLQKDFEESESSKEDHPEASDKDQESEDEDDKKRKAALEKLEKASEDTFLGQASSRCCLIIAANGIKAIDTSVENFATGAWQALGNAWKGGSSFVQKLENSIQQGGLPAAGSVLETGRAFTAKGIEVLEYVGKETVDLLITESGMEIDKNAGEGGNGTEEDQLLEEVTFDRCFYIYGGPEQLEELEALSNHYALLFNRRKAKLSTEEKTAYDTKLKEVQQMLILDNKAEAEKGKNVPDSTDDEIKSFHNSSVSKAAEMAAGFANALAGLSPSDIVQRTGGRLDSLHSEGIHRLSEMCCIAVSQLLNLGKSVIHNANKDQDAAADDEDILNIEWPEDSIEKAKTMRMKTQSMTGYLEAVAISFVTGISDVAEAYAAAIKSATADSPETVPEKSIHDKVNSFSEDLHVNRTTAVGKIQDGLHFLAYVILTTSMPAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BQZ4</accession>
    <name>A0AAD5BQZ4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DOG1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_003440</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bZIP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011339">
        <property type="protein sequence ID" value="KAI7727649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BQZ4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000019">
        <property type="entry name" value="BZIP family transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025422">
        <property type="entry name" value="TGA_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45693:SF47">
        <property type="entry name" value="BASIC-LEUCINE ZIPPER DOMAIN, TRANSCRIPTION FACTOR TGA LIKE DOMAIN PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45693">
        <property type="entry name" value="TRANSCRIPTION FACTOR TGA9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14144">
        <property type="entry name" value="DOG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51806">
        <property type="entry name" value="DOG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="DOG1" evidence="9">
        <location>
            <begin position="115"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="11"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="40"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7727649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="330" mass="37973" checksum="305B7A3E916675EE" modified="2024-05-29" version="1">MWDNTYDAHLGSNTNHNSLTSQPDTMLTDNPDSNSQDSLKSSRDSDKSVSYKLQRRLAQNREAARKSRLKKKAYVQDLELGRLKLAKLEHEIERTRKQAAYMDLSNTLHGLLPGIVTFEKNYDQWVIDQNKKECELATTLQNDASDMEVRVIVDDIVNHYQLLFQMKADAAKTDPFSLLCGSWKSPVERLFQWLGGFRPSEILYILMPRFEPLTDTQVVNLSRLRHTCRQAEDALTQGLDKLEQTLAQSVAINVREGGSYETHMSSMIEVHESLENFLNQADHLRHRTLEQMSRILTIRQASRGLLALGEYFQRLRVLNSLWSSRPHGIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BS29</accession>
    <name>A0AAD5BS29_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_016542</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011200">
        <property type="protein sequence ID" value="KAI7728495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BS29"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035251">
        <property type="term" value="F:UDP-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48047">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48047:SF45">
        <property type="entry name" value="SCOPOLETIN GLUCOSYLTRANSFERASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7728495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="154" mass="17090" checksum="ECC1518D37276F62" modified="2024-05-29" version="1">MVKVTCSQLCEIAMALEACDVIFICVIQNDQEMLIPEGFEARIEAKGKWLIIKGWVPQVLILDHKSVCGFLTHCGWNSLFEGVSSGVAMVAWSVMAKQFYNAKLVTDVLQIGVSIGDVEWSATSTCDGVKREEIERAVARVMDVEDGEDMRMRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BSF4</accession>
    <name>A0AAD5BSF4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_019809</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011344">
        <property type="protein sequence ID" value="KAI7727596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BSF4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7727596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="39" mass="4372" checksum="6FBDA2D543ABA71E" modified="2024-05-29" version="1" fragment="single">MSSRLGCLRINDMTGDIGRLILDLSFFAMESLSDHLTLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BTI7</accession>
    <name>A0AAD5BTI7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_024330</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the AP2/ERF transcription factor family. AP2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011008">
        <property type="protein sequence ID" value="KAI7729393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BTI7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00018">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.730.10:FF:000004">
        <property type="entry name" value="AP2-like ethylene-responsive transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.730.10">
        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001471">
        <property type="entry name" value="AP2/ERF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32467">
        <property type="entry name" value="AP2-LIKE ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32467:SF118">
        <property type="entry name" value="ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR RAP2-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00847">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00380">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51032">
        <property type="entry name" value="AP2_ERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="9">
        <location>
            <begin position="161"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="41"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="41"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7729393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="409" mass="45279" checksum="E8F2D601B95227EA" modified="2024-05-29" version="1">MLDLNLNFINYDEPTAFQDLEESGTSNSSVVNADGNNNKSNNIIIISNTNPGASDRDVEEEQEDSSSVAGACESASISCYNFDILKAGDTGARVSKECNEVERSEIMTLFPNSRAKSLLLCPNSNELQEMRLVPVQQEKANVVPNVVGKKSRRGPRSRSSQYRGVTFYRRTGRWESHIWDCGKQVYLGGFDTAHAAARAYDQAAIKFRGVEADINFNLSDYEENMKQMTNLSKEEFVHILRRQSTGFSRGGSKYRGVTSNKCGQWEAQMGQSLGKKAYDKAAIACDGREALTNFETSYGNNHNLNLSLGISTHSLGAGSSRNENLEQPRMNFVHDASRLQQLENPVSALKGPPSWSHVYPTFVPNYEHTPFDPINGMSSQVDSFIGEGLDYRVGLVLVVSGWIHATNNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BTM9</accession>
    <name>A0AAD5BTM9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DYW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_012903</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. PCMP-H subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011254">
        <property type="protein sequence ID" value="KAI7728211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BTM9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000344">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000325">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein At4g14820"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032867">
        <property type="entry name" value="DYW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF409">
        <property type="entry name" value="DYW DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14432">
        <property type="entry name" value="DYW_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="5"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="137"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="238"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="DYW" evidence="4">
        <location>
            <begin position="455"/>
            <end position="547"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7728211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="547" mass="61320" checksum="99FE1AEE6C872FD6" modified="2024-05-29" version="1">MPKRNVMSCNILIGGFVQNGELGNARKVFDEMPDRNIATWNGMIAGLTDFECNEEALRVLYEMRGSGFYLDEFTLGSALRGCAGLRSLNAGRQIHAYAVKYGLEVNVVVGSSLAHMYIKSGSLAEGENVIKSMPVHSVAVYNTLITGRAQSGCNEEALNQYHIMKISGFKPDKITFVTVLSSCSQLATLGQGQQIHAEAIKSGATSSVSVLSSLISMYSRCGCFKDALKSFSETKIHDVVLWSSMIAAYGFHGMGKEAVELFNRMEKEELVPNDVTFLSLLYACSHCGLKDEGIKIFDLMTEKYKLAPQLEHYTCVVDLLGRSGRLEEAENVIRSMPVNVKADSIIWKTLLSACRKYKNADMAKKIAEEVIRVDPHDSASYVLLSNIQASAKQWHGVSDYRKAMRDKRVKKEPGVSWFEMKNQVHQFCMKDKSHPDSEKIKLYLKELTGEMKLLGYKPDYGSILHDMDLEEKEDDLGQHSEKLAIAFALMNTPDCIPIRIMKNLRVCNDCHVAIKYISAIKKREIIVRDTSRFHHFKEGQCSCGDYW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BU72</accession>
    <name>A0AAD5BU72_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_017989</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011239">
        <property type="protein sequence ID" value="KAI7728299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BU72"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23798">
        <property type="entry name" value="UBCc_UBE2I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000038">
        <property type="entry name" value="SUMO-conjugating enzyme SCE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="5">
        <location>
            <begin position="5"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="3">
        <location>
            <position position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7728299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="160" mass="18030" checksum="3D1F98EAD4E5E07B" modified="2024-05-29" version="1">MSGGIARGRLTEERKSWRKNHPHGFVAKPETLPDGSVNLMIWHCTIPGKTGTDWEGGYYPLTLHFSEDYPSKPPKCKFPPGFFHPNVYPSGTVCLSILNEDSGWRPAITVKQILVGIQDLLDSPNPADPAQTDGYHLFIQDTVEYKRRVRLQAKQYPPLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BU98</accession>
    <name>A0AAD5BU98_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">K Homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_011069</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010968">
        <property type="protein sequence ID" value="KAI7729592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BU98"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd22462">
        <property type="entry name" value="KH-I_HEN4_like_rpt5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22459">
        <property type="entry name" value="KH-I_PEPPER_rpt1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22460">
        <property type="entry name" value="KH-I_PEPPER_rpt2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10288">
        <property type="entry name" value="KH DOMAIN CONTAINING RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="52"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="220"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="311"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="474"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="127"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="392"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="420"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="420"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7729592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="548" mass="58405" checksum="9B707BA9D3ED1F45" modified="2024-05-29" version="1">MSFLHQPTHDDDHNNNNITYEVSDAQEALVRVFERILTVAAESDGCYYAPGGVVSCRLLVSTEVVGGLIGKGGRVVEKIKRDTGCKIRVFSQEWLPGCAKPADELVEIEGGVVAVKRALIAVSGRIQDCPPPDKSRNGFGKPDNRLQNGHVDPFHQARPNNGSNPMMPNRHVDHFAPHNGLNPVSSNRHMDSLPPASSADYASGGSRFGPLGAETSPNQHLIAFRILCHGDLAGGVIGSGGTIVRALENQTGASISVAYPVPGSVERLITITATESPESQNSPAQNAVILVFNRFVETAYQKGLDSPLSGTPISARLVIPHYQMGCLLGRGGSIMADMRKVTGAFIKIVNGSNFPGCASETDEVVLMTGEFVNVRDALYSVTTRLRKHLFASSSKTHRRGMGQPPVDMHSSLTQSMDNLRLSNNVDHPSSSGPWQSQAGAGNSMNGHDSDRGSTSGKGGSGIELVRGGRSAIITNTTFEIMVPENDIGCVYGENGSNLTRLRQISGAQVVVHEPGSRNADYVVVISGTPDQTQSAQSLLQAFMLADQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BY12</accession>
    <name>A0AAD5BY12_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_027138</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010482">
        <property type="protein sequence ID" value="KAI7731324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BY12"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000032">
        <property type="term" value="P:regulation of secondary shoot formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017888">
        <property type="entry name" value="CYC/TB1_R_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017887">
        <property type="entry name" value="TF_TCP_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005333">
        <property type="entry name" value="Transcription_factor_TCP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31072">
        <property type="entry name" value="TRANSCRIPTION FACTOR TCP4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31072:SF148">
        <property type="entry name" value="TRANSCRIPTION FACTOR, TCP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03634">
        <property type="entry name" value="TCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51370">
        <property type="entry name" value="R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51369">
        <property type="entry name" value="TCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TCP" evidence="8">
        <location>
            <begin position="46"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="R" evidence="9">
        <location>
            <begin position="170"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="133"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7731324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="300" mass="34597" checksum="A03E1AF51A042CA9" modified="2024-05-29" version="1">MHPSYINCNTYFCYNHHEQQINFNPTNMHSTMKKSSNSPVVGKSLRKDRHSKINTARGPRDRRMRLSLDVAKKFFKLQDMLGFDKASNTIEWLLTNSQQAIQDLYNQQLSCSLMVVSNSTSSASECEVLSASIDDQSTEKTEVEKMINKKKKKPERVVRKSVYVDHSLAKESREKARARARKRATEKRNTKLGYYDGAANCRQFSKLRPNLDQVLMDQNTNRLGSWIPFEENHVQLTDQPEYPSSHFQFKQGNIVGDSSSMMSSNWSPLFNYQHNPGLSHDQHQFSDLQILGKPWEGINN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BYI8</accession>
    <name>A0AAD5BYI8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_029570</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010315">
        <property type="protein sequence ID" value="KAI7732093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BYI8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00293">
        <property type="entry name" value="USP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000268">
        <property type="entry name" value="probable receptor-like serine/threonine-protein kinase At5g57670"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000284">
        <property type="entry name" value="Putative receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046958">
        <property type="entry name" value="RBK1/2/STUNTED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47987">
        <property type="entry name" value="OS08G0249100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47987:SF11">
        <property type="entry name" value="RECEPTOR-LIKE CYTOSOLIC SERINE_THREONINE-PROTEIN KINASE RBK1 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="359"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="280"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="283"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="310"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7732093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="766" mass="85506" checksum="74DB6FCF69EC99C9" modified="2024-05-29" version="1">MTFETVIHIHSHNNNNNNHGHDHDHGHQGVESKNVLVGIRFDECGRELLDWAMVKVADVGDRVIAINVCRNSDSVSKYKTLLDDYLADYEGLCDHKQVDLTGQVLRGNSVKKVLVREAKFYSAAAVIVGVSKIKAFGGWVSIAKYCAKKLPLATEVLALHNGKVVFKRFSNGELSVSMTDPKPSFYLIGNTRLKDTQSEFCESEASDMGRHSVEGAQSLKHGELNSFESRKKALSSVSVVIEDFAHQRPGWPLLRASSVLTPSAREARKMSVVKWVMNLPNRSAPGTPRTTSSSSTEVSPKSLRSDSRSECSIFTNTSNESNTPKSHELPEILNLLLKTNSSRCQWIGFDLLKASTSHFSSENLIGKGGCHRVYKGIFPDGKSAAVKIRKSSKEAWKDYILEIDIMTSLDHKNITPLLGVCVEDDNLISVYDLVPRGNLEDNLHGVMKDTSVLSWEIRLNIAIGVAEALNYLHKECLRPVIHRDIKSSNILLSDEFEPQLSDFGLAIWGPTTSPFFSHSDVVGTFGYLAPEYFMYGKVSEKIDVYSFGVVLLELLTRKRPISSDPVKGEDSLVMWAKPRLEKGDLASILDVDLDQEVNKSEIVRMALAAMLCLTRSARRRPTMSQVIRILRGEHDLDERACQNEWEVSCNNDGGDDGDDDDEVYPESNAESHLSLAFLDVEEKSTWFGSVDVKQNARVTLEGYLKGRQAVEIWQRILSWIKIPYGQMGDTIKERMESIGGLAALLNRKSIIHAIYVTTLEHLEHKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BYT2</accession>
    <name>A0AAD5BYT2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_002541</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010585">
        <property type="protein sequence ID" value="KAI7730954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BYT2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7730954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="63" mass="6889" checksum="F3D003E5FCA1AAAB" modified="2024-05-29" version="1" fragment="single">SLAVQDYEYTNPLSLCSLFLVCESAAAAATTTWLLAWELLNQMVVVDGEGDRNVGPKFLRLAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BZS1</accession>
    <name>A0AAD5BZS1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Bet v I/Major latex protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_013528</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010162">
        <property type="protein sequence ID" value="KAI7732768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BZS1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000916">
        <property type="entry name" value="Bet_v_I/MLP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051761">
        <property type="entry name" value="MLP-like_ligand-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31907">
        <property type="entry name" value="MLP-LIKE PROTEIN 423"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00407">
        <property type="entry name" value="Bet_v_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01037">
        <property type="entry name" value="Bet_v_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bet v I/Major latex protein" evidence="1">
        <location>
            <begin position="2"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7732768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="96" mass="11048" checksum="F21961075AA78471" modified="2024-05-29" version="1">MAVDGKPQVAKQVIEAVDEENKSVRFKVTEGDIVEAYKTFAVTIRVDTHEEENVVTWTLDYEKVNEYIPNPDRLMELALNVTKDVETYLLLHLQPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C0I9</accession>
    <name>A0AAD5C0I9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">STAS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_005625</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010167">
        <property type="protein sequence ID" value="KAI7732755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C0I9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07042">
        <property type="entry name" value="STAS_SulP_like_sulfate_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.750.24:FF:000002">
        <property type="entry name" value="Sulfate transporter 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.24">
        <property type="entry name" value="STAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011547">
        <property type="entry name" value="SLC26A/SulP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001902">
        <property type="entry name" value="SLC26A/SulP_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00815">
        <property type="entry name" value="sulP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11814">
        <property type="entry name" value="SULFATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01740">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00916">
        <property type="entry name" value="Sulfate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52091">
        <property type="entry name" value="SpoIIaa-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50801">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="112"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="165"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="246"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="275"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="334"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="367"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="404"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="429"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="domain" description="STAS" evidence="7">
        <location>
            <begin position="516"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7732755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="644" mass="70958" checksum="C39107D8A252119B" modified="2024-05-29" version="1" fragment="single">NIYTLSSTTSFTHLFVYDPMQPMEVSFAAPRSFATTFKSDLKETLFPDDPFHDFDGKTLPEKAKKCIRYFVPVFQWLPSYTLKFFMYDLLAGITIASLAIPQGISYAKLANIPPIIGLYSSFVPPLIYAVFGNSKHIAVGTVASSSLIIAGAIEKKVHEADDPQLYLNLIFTATLVSGVIQLILGVFRLGILVDFLSHSTITGFMGGAATIICLQQLKGIFGLKHFTTQTDVVSVTKAIFKNKHEIRWECTLVGIIFLIFLHLAKMVKQKKPKLFWVSAIAPMVVVAIGCIFAYVADVEKYGIAIVGPLKKGINPPSLKNLDFDSKYISAPIEAGIITALVALAEGIAIGRSFALMVNQQIDGNKEMIAFGLMNIVGSLTSCYITTGPFSKTAVNYNAGCKTQMANVVMAVCMLLTLFFLAPFFSYTPLVVLSAIIMSAMLGLIEYEKAYHLFKTDKFDFLICMAAFLGVAFISMDKGLMLSVGLALVRALLYVARPASSKLGNIPDTSLYRDVEQYPGAISIPKTLVLQLGSPIYFANASYIRERILRWVRDEKASKDFEGDDVEYVLLDLGGVTSIDITGVETLIEIRRSLEVQEIKVVIVNPRLEVMEKLIVTNFVDIIGKDHIFLSIQDAIEGCNFSFSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C3V1</accession>
    <name>A0AAD5C3V1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Trichome birefringence-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_031964</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PC-esterase family. TBL subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009873">
        <property type="protein sequence ID" value="KAI7733958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C3V1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016413">
        <property type="term" value="F:O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029962">
        <property type="entry name" value="TBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026057">
        <property type="entry name" value="TBL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025846">
        <property type="entry name" value="TBL_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32285:SF42">
        <property type="entry name" value="PROTEIN TRICHOME BIREFRINGENCE-LIKE 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32285">
        <property type="entry name" value="PROTEIN TRICHOME BIREFRINGENCE-LIKE 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13839">
        <property type="entry name" value="PC-Esterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14416">
        <property type="entry name" value="PMR5N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042236053" description="Trichome birefringence-like N-terminal domain-containing protein" evidence="7">
        <location>
            <begin position="28"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="domain" description="Trichome birefringence-like N-terminal" evidence="9">
        <location>
            <begin position="54"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="Trichome birefringence-like C-terminal" evidence="8">
        <location>
            <begin position="107"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF14416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7733958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="370" mass="42061" checksum="6A5D32A36EF1C291" modified="2024-05-29" version="1">MDFGHRRCLLLIGVIFLLQVQLYLVVSLPETQVPLTSSNNISTSNSQRGRELLGCDLFKGRWVVDNSYPLYSASSCPFIDPGYDCLKHGRKDTQYLKYSWKPDSCILPRFNGLDLLRKWRGKKVMFVGDSLSENMWQSLACLLHASVPRSRPTLKHGEPLTTLTFQEYGVTIFLYRSTYLVDIVREKIGRVLKLDSIQAGNAWKGMDVLVFNSWHWWTHTGRSKPWDYMQYGSTIVKDMNRYEAYFKGMTTWAKWIDLNVNPAKTRVFFQGISPSHYVGQEWGSPSKSCNGQAQPLQGSTYPAGSPKALGVLKKVLASVKKPVYLLDITTLSQLRKDAHPASYGYSGVDCSHWCLPGLPDTWNQLLYASF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C3W5</accession>
    <name>A0AAD5C3W5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">galactinol--sucrose galactosyltransferase</fullName>
            <ecNumber evidence="2">2.4.1.82</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_004078</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Transglycosidase operating by a ping-pong reaction mechanism. Involved in the synthesis of raffinose, a major soluble carbohydrate in seeds, roots and tubers.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>alpha-D-galactosyl-(1-&gt;3)-1D-myo-inositol + sucrose = raffinose + myo-inositol</text>
            <dbReference type="Rhea" id="RHEA:20161"/>
            <dbReference type="ChEBI" id="CHEBI:16634"/>
            <dbReference type="ChEBI" id="CHEBI:17268"/>
            <dbReference type="ChEBI" id="CHEBI:17505"/>
            <dbReference type="ChEBI" id="CHEBI:17992"/>
            <dbReference type="EC" id="2.4.1.82"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolases 36 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.82" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010009730">
        <property type="protein sequence ID" value="KAI7734429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C3W5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047274">
        <property type="term" value="F:galactinol-sucrose galactosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000129">
        <property type="entry name" value="Probable galactinol--sucrose galactosyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008811">
        <property type="entry name" value="Glycosyl_hydrolases_36"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31268">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31268:SF29">
        <property type="entry name" value="GALACTINOL--SUCROSE GALACTOSYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05691">
        <property type="entry name" value="Raffinose_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7734429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="690" mass="76174" checksum="D39A8CA660D18839" modified="2024-05-29" version="1">MTVGAGISVSDGNLNVFGTPVLTDVHHNVFVTSSTDDGIINGAFIGVRSEQTGSRMVFPVGKLEGLRFMCVFRFKLWWMTQRMGNCGQDIPTETQFLIVETREGSHFGDDNEIEDGSHQSALYTVFLPILEGDFRAVIQGSSNNELEICLESGDPAVKEFEGSHLVFVAAGPDPFDVITNAVKSVERHLQTFSHRERKKMPDMLNWFGWCTWDAFYTDVTSEGVKQGLESFQKGGISPKFVIIDDGWQSVDMDPTSVEAKADNTANYANRLTDIKENHKFQKYGKEGHSVEDPSMGLRHVVTDIKDHHDVKYVYVWHALTGYWGGVRPGTGQYDSKLSFPISSPGLECNENCDAFIAISKNGLGLVDPEKVFNFYNELHSYLASAGVDGVKVDVQNILETLGAGHGGRVKLTRKYHQALEASVARNFPDNGIISCMSHNTDGLYSAKRTAVMRASDDFWPRVTASHTVHIASVAYNTVFLGEFMQPDWDMFHSLHPMAEYHGAARAVGGCAIYVSDKPGHHDFNLLKKLVLPDGSILRAKLPGRPTRDCLFTDPTRDGKSLLKIWNLNNFTGVVGVFNCQGAGWCKDGKRILTHDEKPSTITGVIRARDVNYLSKVADSTWDGDAVVYSHLGGALSYLPKNSSIPVTLKPREYEVFTVVPISKLSCGVGFAPIERLHEVAGLSVPKVEYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C5X1</accession>
    <name>A0AAD5C5X1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">anthranilate synthase</fullName>
            <ecNumber evidence="4">4.1.3.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">M8C21_018955</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">AA19_3_7</strain>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21732"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="4.1.3.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 1/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterotetramer consisting of two non-identical subunits: a beta subunit and a large alpha subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.27" evidence="4"/>
    <dbReference type="EMBL" id="JAMZMK010009423">
        <property type="protein sequence ID" value="KAI7735888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C5X1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004049">
        <property type="term" value="F:anthranilate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01743">
        <property type="entry name" value="GATase1_Anthranilate_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000027">
        <property type="entry name" value="Anthranilate synthase beta subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050472">
        <property type="entry name" value="Anth_synth/Amidotransfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006221">
        <property type="entry name" value="TrpG/PapA_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00566">
        <property type="entry name" value="trpG_papA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43418:SF4">
        <property type="entry name" value="MULTIFUNCTIONAL TRYPTOPHAN BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43418">
        <property type="entry name" value="MULTIFUNCTIONAL TRYPTOPHAN BIOSYNTHESIS PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00097">
        <property type="entry name" value="ANTSNTHASEII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00096">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="10" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="12" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-0934">Plastid</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="8" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="Glutamine amidotransferase" evidence="14">
        <location>
            <begin position="57"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAI7735888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="262" mass="28936" checksum="FF921F41FEC4C23F" modified="2024-05-29" version="1">MPAFTLSQWPSIQLKSLQLPQKTEYYNNTLITRKCLAVSVKASSSSSIKKNNNPVIVIDNYDSFTYNLCQIQLLLSNSQKYIGELGCNFEVYRNDEITVDELRRKNPRGIVISPGADKGISVATILELGPTVPLFGVCLGLQCMGEAFGGKLVRSASGPVLGKSSLVYYKEDGEEGLFAGLSNPFAAARYHRFVIDKETFPSDALEITAWTDDGLIMAARHKVYTHLQGVQFHPESIITDEGKKIVGNFIKLIENMEADSQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C7Y8</accession>
    <name>A0AAD5C7Y8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Vacuolar protein sorting-associated protein 54 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_024136</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009330">
        <property type="protein sequence ID" value="KAI7736223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C7Y8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990745">
        <property type="term" value="C:EARP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040047">
        <property type="entry name" value="VPS50"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019515">
        <property type="entry name" value="VPS54_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13258">
        <property type="entry name" value="SYNDETIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13258:SF0">
        <property type="entry name" value="SYNDETIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10475">
        <property type="entry name" value="Vps54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0653">Protein transport</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Vacuolar protein sorting-associated protein 54 N-terminal" evidence="4">
        <location>
            <begin position="137"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF10475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7736223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="336" mass="37558" checksum="332DE53FA994630B" modified="2024-05-29" version="1" fragment="single">MHPSSFSASSPPPPPPFALIPLALNHDNLTPEGFGLSRFFFLGSLLLSPTTGPLDDAGDAMDFSKVGEKLLSSVRSARSLAFLPSTSDRPQVPERAAAAATIARALASIPPHQRQNISSSSEELSSIYGSEPSKQVLEELEEEFYEEEFDPVRHTLENLPYEESDLAYFEEKAALRLLQLDKVTENLSRQVMEHHEVMVKGMDLVGELEKDLKVANVICMNGRRHLTSSRNEVSRDLVVNTSSKRKQTLLDILQILAALRRAKEMQAELDTHVNEENFFKAFQVLSEYLQLLDSLSDLLAIQEMSRGVEIWLGRTLQKLDSLLLGVCQDFKETSFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C8E1</accession>
    <name>A0AAD5C8E1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_033677</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="JAMZMK010009233">
        <property type="protein sequence ID" value="KAI7736610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C8E1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000008">
        <property type="entry name" value="ATP-dependent RNA helicase RhlB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="7">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="8">
        <location>
            <begin position="93"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7736610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="342" mass="37685" checksum="403014902E19AECA" modified="2024-05-29" version="1">MWLFKFLALDEADHMLDMGFEPQIRKIVERLDMPPSSRRQTMLFSATFPTEIQRMASDFLKNYIFLSVGRVGSSTDLIVQKVVFVEDTEKREYLRNLLHDQKAKGNLGKNALTLIFVETKRSADSLEEWLCRMGFPATAIHGDKVQFERERALRSFKNGVSPILVATDVASRGLDIPRVAHVINFDLPRDIDSYVNRIGRTGRAGKSGLATAFFNAKNSGVAKLISELMKEAHQEAPGWLDQYVESYSSSSDYRRGSSRFGGRDFRSGSDGSYGTADYGGTAAYGGNTDYNGTAAAYGGNTDYGGTSYGSYEYYAPPPVAAGFSDSYGGYGGYESVAASGWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CAU1</accession>
    <name>A0AAD5CAU1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_031913</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008895">
        <property type="protein sequence ID" value="KAI7738015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CAU1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7738015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="96" mass="11002" checksum="468F076183A1E025" modified="2024-05-29" version="1" fragment="single">MVEDGNNMVTLLFIELGIKDDPTWRSTGKRVFDSENLAYLMSINLVQAPGKYHQALLSIYNHLNPRLEDQSNLLGDCRLSRKMLLHTLSSRSLNRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CBG4</accession>
    <name>A0AAD5CBG4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">MADS-box transcription factor 23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_002265</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008711">
        <property type="protein sequence ID" value="KAI7738856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CBG4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00265">
        <property type="entry name" value="MADS_MEF2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1810.10">
        <property type="entry name" value="Transcription factor, MADS-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050142">
        <property type="entry name" value="MADS-box/MEF2_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033896">
        <property type="entry name" value="MEF2-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002487">
        <property type="entry name" value="TF_Kbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002100">
        <property type="entry name" value="TF_MADSbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036879">
        <property type="entry name" value="TF_MADSbox_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48019">
        <property type="entry name" value="SERUM RESPONSE FACTOR HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01486">
        <property type="entry name" value="K-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00319">
        <property type="entry name" value="SRF-TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00404">
        <property type="entry name" value="MADSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00432">
        <property type="entry name" value="MADS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55455">
        <property type="entry name" value="SRF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51297">
        <property type="entry name" value="K_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00350">
        <property type="entry name" value="MADS_BOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50066">
        <property type="entry name" value="MADS_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MADS-box" evidence="7">
        <location>
            <begin position="2"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="K-box" evidence="8">
        <location>
            <begin position="88"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="88"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7738856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="257" mass="29360" checksum="FC20DA79CCEA3365" modified="2024-05-29" version="1" fragment="single">IMGRGKIVIRRIDNSTSRQVTFSKRRSGLLKKAKELAILCDAEVGVVIFSSTSKLHEYSSTSMKSVIERYHKAKEDQQLTTSSPVSDVRFWQREAAMLRQQLQSLQENHRRMMGEELSGLAVKDLQGMESQLEMSLRSIRMKKDQLLFEEIQELNRKGNLIHQENVELCEQVNRIREENTELYKKAYGIREADATNGNLFLPNALSIRENPPATIHLQLSQPDQHEATEASLQSTNLGIKKKMTGHGSNAYIVLMYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CBN0</accession>
    <name>A0AAD5CBN0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_007350</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008692">
        <property type="protein sequence ID" value="KAI7738961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CBN0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008521">
        <property type="entry name" value="Mg_trans_NIPA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05653">
        <property type="entry name" value="Mg_trans_NIPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="25"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7738961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="128" mass="13563" checksum="74EE9495B438B68B" modified="2024-05-29" version="1">MKAGVSGLRAGSGGHSYLNLDATFVVGWMVSLVLGEVANFAVYAFAPAILVTHLEALSPASPNSCGDPNSRHLKLQHVICDDNDSCLAAGSDTWQCVEGGRVVMMTLAVTIKMAIEWVVMVTPNIFII</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CBX3</accession>
    <name>A0AAD5CBX3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_013274</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008958">
        <property type="protein sequence ID" value="KAI7737779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CBX3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7737779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="96" mass="10817" checksum="6A566E8C11BAE973" modified="2024-05-29" version="1">MKIEAQDLSGMTPETAKEGTSNVQAVPEVDGTDEDHEEDVNLIRGLVLAISNLYESTSYVNESHPILHHVNRLQKPYKSGEDIHKQGRLSEYNRLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CC36</accession>
    <name>A0AAD5CC36_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_000964</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008930">
        <property type="protein sequence ID" value="KAI7737869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CC36"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044795">
        <property type="entry name" value="THA8L-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46870">
        <property type="entry name" value="PROTEIN THYLAKOID ASSEMBLY 8-LIKE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46870:SF2">
        <property type="entry name" value="PROTEIN THYLAKOID ASSEMBLY 8-LIKE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7737869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="156" mass="18259" checksum="751EF0BEFE3D4561" modified="2024-05-29" version="1">MAAFGGRLRSKIQLSVFSLVNHDITRIPILKGRVCSYYYSSSSTIQLVCNHPTRCFEDVWHSQQQSYRYLRPCYYHDGRPRGSLWRGKKLIGKEALFVILGLKRVKDDEEKLDNFIKVHVSRLLKMDMVAVLNELERQEEVDLAVKVLLISLYYFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CC96</accession>
    <name>A0AAD5CC96_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Mitochondrial succinate-fumarate transporter 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_030957</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008739">
        <property type="protein sequence ID" value="KAI7738735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CC96"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005469">
        <property type="term" value="F:succinate:fumarate antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000082">
        <property type="entry name" value="Mitochondrial succinate-fumarate transporter 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049563">
        <property type="entry name" value="TXTP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45788">
        <property type="entry name" value="SUCCINATE/FUMARATE MITOCHONDRIAL TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45788:SF2">
        <property type="entry name" value="SUCCINATE_FUMARATE MITOCHONDRIAL TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="15"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="109"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="212"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7738735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="302" mass="32966" checksum="E6376D9D17C5BF9A" modified="2024-05-29" version="1">MDQPGSNPTQTKPVIPAYVKAISGSLAGIVEAACLQPTDVIKTRLQLDRAGVYKGILHCGTTTVKTEGVRALWKGLTPFSTHLTLKYALRMGSNAVLQSAFKDSDTGKISVGGRFLSGFGAGAIEAVAIVTPFEVVKIRLQQQKGMSQEALKYKGPIHCARLIMREEGLAGLWSGVGPTIMRNGTNQAFMFTAKNTFDKVLWRKDEGDGKVLQPWQSMMSGFLAGIVGPVCTGPFDVVKTRLMAQSGEMKYKGMVHAFSSIYREEGLRALWKGLIPRLMRIPPGQAIMWGVADQYKEYPCVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CEU4</accession>
    <name>A0AAD5CEU4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_018173</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008373">
        <property type="protein sequence ID" value="KAI7740672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CEU4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7740672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="118" mass="13357" checksum="DB74C9CC597B3644" modified="2024-05-29" version="1">MVILILILSNSDVLNKTDTDLTVFEASGILSNFLEFRCTQGLRIPVFEASVFKTALLSDSRGFSAYKRDNLLPIEQLFLRLREAHFVIADGEGGFVKQAKVAVLPQRLELRFILVDRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CG81</accession>
    <name>A0AAD5CG81_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Strictosidine synthase conserved region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_009433</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the strictosidine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008454">
        <property type="protein sequence ID" value="KAI7740360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CG81"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018119">
        <property type="entry name" value="Strictosidine_synth_cons-reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10426:SF68">
        <property type="entry name" value="OS07G0614000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10426">
        <property type="entry name" value="STRICTOSIDINE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20067">
        <property type="entry name" value="SSL_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03088">
        <property type="entry name" value="Str_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63829">
        <property type="entry name" value="Calcium-dependent phosphotriesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0926">Vacuole</keyword>
    <feature type="domain" description="Strictosidine synthase conserved region" evidence="5">
        <location>
            <begin position="157"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7740360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="305" mass="33375" checksum="6D9C14BC81D57AC4" modified="2024-05-29" version="1" fragment="single">MEFGVIAVSFVLVSALAVTLQVFFYSPLSPDILELPHDHSSHLFAPNNDLQKVIKIGEGILNKPEGLCLDQKGMLYTATRDGWIKRLHTNGTCENWKKIHDRDTLLGLIVTKAGDLVVCDTEEGLIKVSEDGAVTAIATHMNGENIKFADDVAEAGDGSLYFSIASTKFGLHDWPLDVLEAKPHGQLLKYDPATMETSLVLDRLGFANGVTVSSDQEFLVVCESWKCLKYWLKEEMKGKVEVFIDNLPGAPDNINVAPDGAVERSAMALNVGSDGKIIKRLDDPTGEVMAFVTSVLEFNGKLYFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CGG1</accession>
    <name>A0AAD5CGG1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mitogen-activated protein kinase</fullName>
            <ecNumber evidence="9">2.7.11.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_028034</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="9">Activated by threonine and tyrosine phosphorylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.24" evidence="9"/>
    <dbReference type="EMBL" id="JAMZMK010008533">
        <property type="protein sequence ID" value="KAI7740016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CGG1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004707">
        <property type="term" value="F:MAP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010225">
        <property type="term" value="P:response to UV-C"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000013">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000046">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003527">
        <property type="entry name" value="MAP_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01351">
        <property type="entry name" value="MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="40"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7740016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="372" mass="42778" checksum="11AE163D286E2E36" modified="2024-05-29" version="1">MAELPGGAQYTDFPAVPTHGGQFVRYNIFGNEFEITSKYLPPIMPIGRGAYGIVCSMLNSETNEMVAMKKIMNAFDNYMDAKRTLREIKLMRHLDHENVIAIKDVVPPPVRREFSDVYIATELMDTDLHQIIRSNQTLSEEHCQYFLYQLLRGLKYIHSAKVIHRDLKPSNLLLNANCDLKICDFGLARPNADNDFMTEYVVTRWYRAPELLLNSSDYSAAIDVWSVGCIYMELMNRKPLFPGKDHVHQMRLLTELLGTPSESDLGFIKNEDAIRFIAQLPQHPRKSLPKTFPHVHPLAIDLVEKMLTFDPAKRITVEEALAHPYLERLHDVEDEPICPNPFSFDFEQKVLGEKQIKDLIYQEAIAHNPDYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CH94</accession>
    <name>A0AAD5CH94_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RlpA-like double-psi beta-barrel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_000175</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the kiwellin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008186">
        <property type="protein sequence ID" value="KAI7741479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CH94"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd22270">
        <property type="entry name" value="DPBB_kiwellin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="entry name" value="RlpA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039271">
        <property type="entry name" value="Kiwellin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33191:SF77">
        <property type="entry name" value="RIPENING-RELATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33191">
        <property type="entry name" value="RIPENING-RELATED PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24300">
        <property type="entry name" value="KWL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="Barwin-like endoglucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041914467" description="RlpA-like double-psi beta-barrel domain-containing protein" evidence="5">
        <location>
            <begin position="23"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7741479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="183" mass="19841" checksum="B323F8F458EEB6EF" modified="2024-05-29" version="1">MKRFHFIMLFVLLAAILVQINAQTCKPSGGIRGRKPPSGQCNKENDSDCCVQGKFYTTYTCSPTVTSATKATLTINSFQKGGDGGGPSECDNQYHSDDTPVVALSTGWYKGGDRCHKFITINGNGRSVKAMVVDECDSTMGCDADHDYQPPCPNNIVDASKAVWKALGVSEDNWGDLDITWSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CJX8</accession>
    <name>A0AAD5CJX8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Early nodulin-93-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_007496</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007770">
        <property type="protein sequence ID" value="KAI7743222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CJX8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005050">
        <property type="entry name" value="Enod93"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33605">
        <property type="entry name" value="EARLY NODULIN-93"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33605:SF3">
        <property type="entry name" value="EARLY NODULIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03386">
        <property type="entry name" value="ENOD93"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="74"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7743222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="114" mass="12619" checksum="2C5324C5D6AD4DE9" modified="2024-05-29" version="1">MGIPMDIRDAWVNHRSHLVIPSPDEDLKFLRSQMCTQEGVREGVKSASIACVATAVPTLIAVRTIPWAKANLNYTAQALIISAVSIAAYFITADKTILECARRNTRTLYDKQQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CK31</accession>
    <name>A0AAD5CK31_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Histone H2A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_006844</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the histone H2A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008090">
        <property type="protein sequence ID" value="KAI7741996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CK31"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00074">
        <property type="entry name" value="HFD_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002119">
        <property type="entry name" value="Histone_H2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032454">
        <property type="entry name" value="Histone_H2A_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23430">
        <property type="entry name" value="HISTONE H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16211">
        <property type="entry name" value="Histone_H2A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00620">
        <property type="entry name" value="HISTONEH2A"/>
    </dbReference>
    <dbReference type="SMART" id="SM00414">
        <property type="entry name" value="H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0158">Chromosome</keyword>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="2">
        <location>
            <begin position="34"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="Histone H2A C-terminal" evidence="3">
        <location>
            <begin position="101"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7741996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="164" mass="18789" checksum="E2C63F054F7DF7D8" modified="2024-05-29" version="1">MKNKLMEWSKEKNDVGGRNGPRKKVVPVIRVRFLFPVNRFIRYSKHVRYTMRVGTDAPIYLVIVLEYLATKILELAGNATRDNKKNRIIPRHVFLAIRNDDELGKLLSAVIIAHGDVVLNINPVLLPKRTAAKEPKSPAKATKSPRKLMIRYVRWVVLMSCDCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CK68</accession>
    <name>A0AAD5CK68_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DYW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_012030</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. PCMP-H subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008019">
        <property type="protein sequence ID" value="KAI7742340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CK68"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032867">
        <property type="entry name" value="DYW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF502">
        <property type="entry name" value="SELENIUM BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14432">
        <property type="entry name" value="DYW_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DYW" evidence="2">
        <location>
            <begin position="84"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7742340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="134" mass="15642" checksum="75E6EA6891790F36" modified="2024-05-29" version="1">MIDLFSQQRNGYASAGKWNKASKVRKFMKKIGVKKEPVCSWVDIENSVHMFVANDENHPLQNEINKMWGKNSEKIKEIGYVPDTIRIKKNIIVCGDYHSAFKYASLVVKREILLRHTNRFHHFHRGSCSCGDYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-02-05" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CL34</accession>
    <name>A0AAD5CL34_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_028173</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007884">
        <property type="protein sequence ID" value="KAI7742835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03213">
        <property type="entry name" value="ABCG_EPDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001533">
        <property type="entry name" value="ABC transporter G family member 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052215">
        <property type="entry name" value="Plant_ABCG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48042">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48042:SF1">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 11-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19055">
        <property type="entry name" value="ABC2_membrane_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="391"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="424"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="465"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="503"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="529"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="618"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="51"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7742835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="674" mass="74871" checksum="38A724D6ABC1684B" modified="2024-05-29" version="1">MEPSHDIKVEKYPQEVEITSHAPSLPYHEGHQRSTHSNGPVQDDTFNGVCLTWKDLWVMVRDGQTGGNRSILQGVTGYARPGEILAIMGPSGSGKSTLLDALAGRLDPKIRKSGDILVNGVKKTLAYGTSAYVTQDDALITTLTVRESVYYSAQLQLPDSMSKAEKKERAEMTIKEMGLQDAMDTRIGGWGAKGLSGGQKRRVSICIEILTRPKLLFLDEPTSGLDSAASYYVMSRIARLDQHEGRTIIASIHQPCSEVFGLFHNLCLLSSGRTVYFGEASSANEFFTLNGFPCPNFQNPSDHFLRTINKDFDEDIELGLAGRKPTEEVIDTLIDSYKSSTTYQEVNGVVSDICKKEDAAHDKNDHAGFSTQSIVLTRRSFVNMHRDLGYYWLRLAIYIALSLGLGTIYYNVGSSYSSIQARCSMLMFVASFLTFMAIGGFPSFVEDMKVFERERLNGHYSTGAFVIANTLSSTPYLLLVSVIPGAVAYYLTGLRDGFDHFAYYASVLFSCMLVVESLMMIVASIVPNYLMGIIMGAGIQGFMMLAGGFFRLPNDLPHPFWKYPLFYIGFHKYAYQGLFKNEFEGVKLLDNEAGVKKIVDGEYILREQWQVEMGYSKWIDLVIISGMAVISRILFLVIIMIVEKVKSGLMAVVFSIXXXXSDDQSSCHTFSLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CLZ4</accession>
    <name>A0AAD5CLZ4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_000809</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007713">
        <property type="protein sequence ID" value="KAI7743610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CLZ4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009873">
        <property type="term" value="P:ethylene-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00018">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.730.10:FF:000001">
        <property type="entry name" value="Ethylene-responsive transcription factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.730.10">
        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001471">
        <property type="entry name" value="AP2/ERF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044808">
        <property type="entry name" value="ERF_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31190">
        <property type="entry name" value="DNA-BINDING DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31190:SF421">
        <property type="entry name" value="ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR ERF110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00847">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00367">
        <property type="entry name" value="ETHRSPELEMNT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00380">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51032">
        <property type="entry name" value="AP2_ERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0611">Plant defense</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="8">
        <location>
            <begin position="179"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="7"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="60"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="324"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="60"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="82"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="147"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="160"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="329"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7743610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="390" mass="42899" checksum="8A04542BF556D7DD" modified="2024-05-29" version="1">MCFIKVANSKDSGDYDRGTTGEGEGGSPPQSPADMFSTEYSMSGELSAMVSALTHVVSGHGSGGGSGGGGFNISPSFRGLDSPSSAYSSSSSGSLAGQKRVRDHQEESVNQFPEQFHQGFYGGFGDFRGGESSSSRVKEEATPMPPSTTVAETATASATPQNPATEPNQPEQTGERPRKYRGVRQRPWGKWAAEIRDPHKAARVWLGTFDTAEAAARAYDEAALRFRGNRAKLNFPENVRLLPQQQIRQTTNIAISHSPMTTQFTIRQQPPVPPHRFQFPDPTMVRDYWQYSQLLQSNVDFRQQQPQQQQQQQQRNLTQQMFSTSTMGSLSSQASNISSSSSNYPLLFPNPSGYLDFRQPGGQNRDDEPDFPFVPSTWPDSDQFQPPPPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CQZ6</accession>
    <name>A0AAD5CQZ6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_016025</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006990">
        <property type="protein sequence ID" value="KAI7746372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CQZ6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7746372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="107" mass="12188" checksum="1390E49D216C5360" modified="2024-05-29" version="1" fragment="single">SLAKREGRVKDYRRLRSLRGGLQRRYSKDEDGHERGPQDPVDALVFASHGQQQRRGQPAILSHPDDHIVTSKNKSCGEDEELMMDNLDFDQMSFSSTKRPTLSTIGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CRI7</accession>
    <name>A0AAD5CRI7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_021171</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006870">
        <property type="protein sequence ID" value="KAI7746956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CRI7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004605">
        <property type="term" value="F:phosphatidate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032049">
        <property type="term" value="P:cardiolipin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015222">
        <property type="entry name" value="Tam41"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09139">
        <property type="entry name" value="Tam41_Mmp37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7746956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="73" mass="7754" checksum="1256C085F1553012" modified="2024-05-29" version="1">MGVGTVLTKAGQNSKLTIGSREEAATLMQKVLRRRVMISSARQAVAGFIAVGAVQGVKYLGKKMTKAWNSSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CSP5</accession>
    <name>A0AAD5CSP5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Trehalose-phosphatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_016735</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">In the C-terminal section; belongs to the trehalose phosphatase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">In the N-terminal section; belongs to the glycosyltransferase 20 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006739">
        <property type="protein sequence ID" value="KAI7747533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CSP5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004805">
        <property type="term" value="F:trehalose-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005992">
        <property type="term" value="P:trehalose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03788">
        <property type="entry name" value="GT20_TPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01627">
        <property type="entry name" value="HAD_TPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000010">
        <property type="entry name" value="Alpha,alpha-trehalose-phosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000052">
        <property type="entry name" value="Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000054">
        <property type="entry name" value="alpha,alpha-trehalose-phosphate synthase [UDP-forming] 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001830">
        <property type="entry name" value="Glyco_trans_20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006379">
        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003337">
        <property type="entry name" value="Trehalose_PPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01484">
        <property type="entry name" value="HAD-SF-IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00685">
        <property type="entry name" value="T6PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788:SF94">
        <property type="entry name" value="ALPHA,ALPHA-TREHALOSE-PHOSPHATE SYNTHASE [UDP-FORMING] 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788">
        <property type="entry name" value="TREHALOSE-6-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00982">
        <property type="entry name" value="Glyco_transf_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02358">
        <property type="entry name" value="Trehalose_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="644"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7747533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="644" mass="73045" checksum="2E970BD778CF3DB8" modified="2024-05-29" version="1" fragment="single">KRFNRVKLGFFLHSPFPSSEIYRTLPVRDELLRALLNSDLIGFHTFDYARHFLSCCSRMLGLSYQSKRGYIGLEYYGRTVSIKILPVGIHLKQLRNVLDLPETESKVAELRDQFQGRTVLLGVDDMDIFKGISLKLLAFENLLTQHPEKRGKVVLVQIANPARGRGRDVLEVQSETHTTRERINRKFGRPGYKPVILIDSPLQFYERIAYYVISECCLVTAVRDGMNLIPYEYVICRQGNDKLDVTLGANPSIPKKSMLVVSEFIGCSPSLSGAIRVNPWNIDAVAEAMDSALVYADAEKQMRHEKHYRYVSTHDVAYWARSFWQDLERSCRDHVRRRCWGIGFGLGFRVVALDPNFRKLSIEHIVSAYKRTKKRAILLDYDGTMTLQSSISTGPSSEVIEIVNSLCQDPKNVVFIVSGKDRVTLTEWFSSCENLGVAAEHGYFFREDKDVEWETCVAVPDFYWKQIAEPVMQLYTETTDGSTIETKESGLVWNYQYADPDFGSCQAKELLDHLESVLANEPVTVKSGQNIVEVKPQGVNKGLVADRLLNTMKHRKMVPDFVLCIGDDRSDEDMFEAITRAIAAPSLSPVAEVFACTVGRKPSKARYFLEDTTEILRMLQGLAAVSEQHSLNNVTKGFQKVVIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CW78</accession>
    <name>A0AAD5CW78_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_012858</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006605">
        <property type="protein sequence ID" value="KAI7748069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CW78"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7748069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="79" mass="8868" checksum="9A161A25A8D4AB18" modified="2024-05-29" version="1">MWAFCVYLESTPVIPQVTMISFVLAWYDGEEGGEMEVTSFVEPLVGEVEASKAVALREADLQKELELMNALTQIEKPRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CXP0</accession>
    <name>A0AAD5CXP0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Acyl-coenzyme A oxidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">M8C21_032953</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">AA19_3_7</strain>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 2,3-saturated acyl-CoA + O2 = a (2E)-enoyl-CoA + H2O2</text>
            <dbReference type="Rhea" id="RHEA:38959"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:58856"/>
            <dbReference type="ChEBI" id="CHEBI:65111"/>
            <dbReference type="EC" id="1.3.3.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the acyl-CoA oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006384">
        <property type="protein sequence ID" value="KAI7749347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CXP0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003997">
        <property type="term" value="F:acyl-CoA oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005504">
        <property type="term" value="F:fatty acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033540">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA oxidase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.10:FF:000007">
        <property type="entry name" value="Acyl-coenzyme A oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.10:FF:000010">
        <property type="entry name" value="Acyl-coenzyme A oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.110.10:FF:000005">
        <property type="entry name" value="Acyl-coenzyme A oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055060">
        <property type="entry name" value="ACOX_C_alpha1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012258">
        <property type="entry name" value="Acyl-CoA_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002655">
        <property type="entry name" value="Acyl-CoA_oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10909:SF352">
        <property type="entry name" value="ACYL-COENZYME A OXIDASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10909">
        <property type="entry name" value="ELECTRON TRANSPORT OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01756">
        <property type="entry name" value="ACOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22924">
        <property type="entry name" value="ACOX_C_alpha1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000168">
        <property type="entry name" value="Acyl-CoA_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11 13" id="KW-0274">FAD</keyword>
    <keyword evidence="7" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="5 11" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="15">
        <location>
            <begin position="183"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase C-alpha1" evidence="16">
        <location>
            <begin position="330"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase C-terminal" evidence="14">
        <location>
            <begin position="525"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="12">
        <location>
            <position position="466"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR000168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000168-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000168-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF22924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAI7749347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="673" mass="75788" checksum="885F499579EB3A28" modified="2024-05-29" version="1">MDRASYRTQILTRHLLTHQPTTTDHLNTTPCISYTPPELNEPRPDFDTLLLRKLLDGQSVEDIDYIFNLMKQSALFCPKERGGKVFIGPDYNQSMEQQREMTMRRIEYLRDQGAFDGWFDKKGPEGDLWRFALAESVSVYDHSLGIKLGVHFFLWGGAIRFMGTKRHHDKWFKPTETYQVKGCFAMTELGHGSNVRGIETITRYDASTGEFIINTPCESAQKYWIGGAANHATHTVVFSQLEINGVNQGVHAFIAQIRDENGNVCPNIRIADCGHKIGLNGVDNGRIWFDNLRIPRENLLNSVADVSPDGQYLSAIKDPDQRFAAFLAPLTSGRVTIAASSMNTTKIGLAIAIRYSLSRRAFSIRPNEPEVLLLDYPSHQRRLLPLLAKTYALSFASNYLKMIYVKRTPESIKTVHVVSSALKAVLTWHCMRTLQECREACGGQGLKTENHVGHLKSEYDVQLTFEGDNNVLMQQISKALLAEFLAAKKRNKPIRGLGLEHLNKPAPIIPTQLTSSTLRTTQFQTDIFCLRERDLLNRFVTEVSQHQAQGESKEHIFTVTYQTAEDLGRAFSDRLILQTYLDVEATVPEGSLKSVLGLMRSMYTTIVLEEDASFLRYGYLSTDNAAVVRKEVLKLCSELRPHALSLVSSFGIPDAFLGPIAFNWVDANAWSSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CYB0</accession>
    <name>A0AAD5CYB0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">glucan endo-1,3-beta-D-glucosidase</fullName>
            <ecNumber evidence="4">3.2.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">M8C21_026291</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">AA19_3_7</strain>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of (1-&gt;3)-beta-D-glucosidic linkages in (1-&gt;3)-beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.39"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <topology evidence="2">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the glycosyl hydrolase 17 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.39" evidence="4"/>
    <dbReference type="EMBL" id="JAMZMK010006189">
        <property type="protein sequence ID" value="KAI7750194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CYB0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042973">
        <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000008">
        <property type="entry name" value="Glucan endo-1,3-beta-glucosidase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1040:FF:000002">
        <property type="entry name" value="Glucan endo-1,3-beta-glucosidase 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000490">
        <property type="entry name" value="Glyco_hydro_17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044965">
        <property type="entry name" value="Glyco_hydro_17_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012946">
        <property type="entry name" value="X8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32227">
        <property type="entry name" value="GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00332">
        <property type="entry name" value="Glyco_hydro_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07983">
        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00768">
        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00587">
        <property type="entry name" value="GLYCOSYL_HYDROL_F17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14 16" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0611">Plant defense</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 17" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042176922" description="glucan endo-1,3-beta-D-glucosidase" evidence="17">
        <location>
            <begin position="18"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="domain" description="X8" evidence="18">
        <location>
            <begin position="365"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU004336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAI7750194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="477" mass="53596" checksum="A5E2F1BB9159C963" modified="2024-05-29" version="1">MARAMVFIWTCYCVVLATKCVNCLGVNWGTQASTPLDPKYVVQMLKDNKIQKVKLFDSDIWTVNHFAGTGIEVMLGIPNLYLKEFSNDFDAVEDYIKENVTEHLRNHGGVNIKYIAVGNEPFLKSYKGAYEKHIFPALKNVQKALNKAGLGNKIKATIPQNADIYDSGSAGPSAGDFRAEIRDIMVKIARFLKQNNCPFMVNIYPFLSLYENDDFPIEFAFFEGGGKGVQDQNIRYTNMFDANLDTLSHSLKKAGVNDIKIIVGEIGWPTNGHKQANIKMAQKFYHGLLQKLSTNKGTPLNPGYIEVYLFSLTDENKKSIAPGFFERHWGIFQYDGQPKFPIDFTGEGNEKMPIGAKDVKYLEKQWCVLNDDMKNLSKVNKDLEFACSLSDCTSLSYGSTCNKLSERKNVSYAFNMYFQMSQQSVEACDFNGAAKITKKNYSTDECLFPVELMSGGRRNVVGMMSFLGGLFSLLVIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D003</accession>
    <name>A0AAD5D003_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_022560</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005885">
        <property type="protein sequence ID" value="KAI7751486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D003"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34213">
        <property type="entry name" value="NUCLEAR TRANSPORT FACTOR 2 (NTF2) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34213:SF2">
        <property type="entry name" value="NUCLEAR TRANSPORT FACTOR 2 (NTF2) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7751486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="222" mass="25333" checksum="4317376B763CCAC8" modified="2024-05-29" version="1">MNPLRSASTLLHYRPLFSIPHSSSSSSSFSTNNLTRSTMETYSKDNNEPKKDQQKETCDNNQSKKVSDDILPHILNIYGSRASPQDFEIYAPNASFEDPLMCAHGVKQIKSAFYSLSKVFKESKIVEYTLEENVLPNGTREILIDNKQYYKFMGRNIDMISLIKLYVENGKVIRHEDCWDKKPLWNTETSKVPLVGKLVEMTKRGSMFATHAMMGFGKDPTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D009</accession>
    <name>A0AAD5D009_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">aminocyclopropanecarboxylate oxidase</fullName>
            <ecNumber evidence="8">1.14.17.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_031747</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>1-aminocyclopropane-1-carboxylate + L-ascorbate + O2 = ethene + L-dehydroascorbate + hydrogen cyanide + CO2 + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:23640"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:18153"/>
            <dbReference type="ChEBI" id="CHEBI:18407"/>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
            <dbReference type="ChEBI" id="CHEBI:58360"/>
            <dbReference type="ChEBI" id="CHEBI:58539"/>
            <dbReference type="EC" id="1.14.17.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Alkene biosynthesis; ethylene biosynthesis via S-adenosyl-L-methionine; ethylene from S-adenosyl-L-methionine: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the iron/ascorbate-dependent oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.17.4" evidence="8"/>
    <dbReference type="EMBL" id="JAMZMK010006124">
        <property type="protein sequence ID" value="KAI7750562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D009"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009815">
        <property type="term" value="F:1-aminocyclopropane-1-carboxylate oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031418">
        <property type="term" value="F:L-ascorbic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009693">
        <property type="term" value="P:ethylene biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.330:FF:000010">
        <property type="entry name" value="1-aminocyclopropane-1-carboxylate oxidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050295">
        <property type="entry name" value="Plant_2OG-oxidoreductases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47991">
        <property type="entry name" value="OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0266">Ethylene biosynthesis</keyword>
    <keyword evidence="6 10" id="KW-0408">Iron</keyword>
    <keyword evidence="3 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0847">Vitamin C</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="11">
        <location>
            <begin position="155"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00050579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7750562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="311" mass="35604" checksum="40767528F675B2EF" modified="2024-05-29" version="1">MPIPVIDFSKLNGEEREKTMAEIANGCEEWGFFQLVNHGISVELLERVKKVTTECYKVEREENFFKDSTPAKLLKELVDKKGEEKLENMDWEDVFLLADDNEWPPKTPGFKETMTEYRTELKKLAEKLMEVMDENLGLPKGHIKKMFNGGDEEKAFFGTKVSHYPPCPHPERVTGLRAHTDAGGVILLFQDDEVGGLEILKDGKWTDVQPIPNVICINTGDQIEVLSNGRYKSVWHRVQAMPNGTRRSIASFYNPSYNATIEPAVQLIQKENKEVNQFGYPKFVFGDYMSVYTEQKFLPKEPRFEAVKAAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D6A7</accession>
    <name>A0AAD5D6A7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF8039 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_025993</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010001697">
        <property type="protein sequence ID" value="KAI7755123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D6A7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058352">
        <property type="entry name" value="DUF8039"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33018">
        <property type="entry name" value="OS10G0338966 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33018:SF35">
        <property type="entry name" value="ULP1 PROTEASE FAMILY CATALYTIC DOMAIN, PAPAIN-LIKE CYSTEINE PEPTIDASE SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26133">
        <property type="entry name" value="DUF8039"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF8039" evidence="2">
        <location>
            <begin position="331"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="304"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="451"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="312"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF26133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7755123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="616" mass="71348" checksum="1293176D39872FB9" modified="2024-05-29" version="1" fragment="single">MTDSTDGCRHDLTDATSIVSQRKTRGVARCKRKLKPEEMYIDLNENNLPIGKLQTYFDSWLGLETRTYFPEYWIQSHKYSKQRWHELWLHIKDSWHFQDDVPEKAIIDKAKKGRTDFISKELWRWYKRKRRNPCLRFSYFDASRWDAFVAAKSTKKAKEISEKARQSALANKNQQRIGRGGYRALEYKFHKVWPPIVDANPHLDDLDNRTRLYLAGRIRKDKQTGTYNTQQYEEQISALIEAEKKMKLDGTYGQGRVDPMIKVFGVEHGGRTRGVSSTIGHTKVKVPKLKRVSEMVEASPIMHMSQKRRVDASPGFPSSSCGSGASHTIYPEIEKLSRCQIFWCHGEKSADEHIADGQVYPTSDRYLDDILIHEHCVKVHVDSVRADYMPMPVPDEALREGVRYMKNTLLKFIQWPRKAIKILDEPTQTRPCDLMPAVELYTPEVMRSAEAKEKQAKAQVTDRNKPKAKRNKETQAKKSKGKGKEKQAEALVTDAEKQAQKVSLALKVVEIREEWYQNMVRQLGAHIRGDWATTFPFSAHENMFPTEAVSFIEAEAILALFANEWVDLTIVFWFTSYFYKLSKKTKLNRCAFFNVNAIIGDLCKTNPKEVEEHILN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D6K8</accession>
    <name>A0AAD5D6K8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_009565</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="JAMZMK010004554">
        <property type="protein sequence ID" value="KAI7754050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D6K8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006970">
        <property type="term" value="P:response to osmotic stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14662">
        <property type="entry name" value="STKc_SnRK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000132">
        <property type="entry name" value="Serine/threonine-protein kinase SRK2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000045">
        <property type="entry name" value="Serine/threonine-protein kinase SRK2E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24343">
        <property type="entry name" value="SERINE/THREONINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24343:SF376">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE SRK2A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="4"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="297"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="316"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="336"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7754050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="352" mass="40597" checksum="D8BD29ACEEBED347" modified="2024-05-29" version="1">MEKYELVKDIGAGNFGVARLMRNKVSKELVAMKYIERGHKIDENVAREIINHRSLRHPNIIRFREVVLTPTHLAIVMEYAAGGELFDRICNAGRFSEDEARYFFQQLISGVHYCHFMQICHRDLKLENTLLDGSPAPRLKICDFGYSKSSLLHSRPKSTVGTPAYIAPEVLSRKEYDGKMADVWSCGVTLYVMLVGAYPFEDQQDPKNFRKTIQRIMAVQYKIPDYVHISQECRHLLSRIFVPNASRRISLKEIKSHPWFLKNLPRELTEAAQAVYYRKENPTFSPQSVEEIMKIVEEARSPPPASRSIGGFGWGPEEDDDDDDKQIETEDDEDEYDKRVKEAHQSGEFFGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D6X8</accession>
    <name>A0AAD5D6X8_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_010128</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010002124">
        <property type="protein sequence ID" value="KAI7754998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D6X8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080043">
        <property type="term" value="F:quercetin 3-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080044">
        <property type="term" value="F:quercetin 7-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926">
        <property type="entry name" value="GLUCOSYL/GLUCURONOSYL TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926:SF870">
        <property type="entry name" value="UDP-GLYCOSYLTRANSFERASE 75B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7754998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="302" mass="34090" checksum="2D9A3FB823E98BE4" modified="2024-05-29" version="1" fragment="single">GSECLKKTILSSAENGTPVTCLVYNLLLPWAAEVACDHNVPLALLWIQPASVFRVYYYYFKGYDKLIKEHCNEPSWSIELPGLPSLKRCDLPSICLPSNTLEHVIYVLKELLYFLDSKEKPKILVNTFDALEEEALEEIDGKINMVGVGPLIPSAFLDGKDPSDRSFGGDLFETSHGYLEWMNTKPEGSIVYISFGTMIVWSKEHKEAMAHALMESKKPFLWTDEDGVFEGEEMRRCTEMVMGGHERGETVRMNAKKWKHLARESIKENGSSYMNLKSFAEEVGSGTEDKVKVMIPSNESKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-10-08" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D8Q8</accession>
    <name>A0AAD5D8Q8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Thaumatin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_020181</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the thaumatin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010002098">
        <property type="protein sequence ID" value="KAI7755014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09218">
        <property type="entry name" value="TLP-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.110.10:FF:000002">
        <property type="entry name" value="Thaumatin-like protein 1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.110.10">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037176">
        <property type="entry name" value="Osmotin/thaumatin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001938">
        <property type="entry name" value="Thaumatin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017949">
        <property type="entry name" value="Thaumatin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31048">
        <property type="entry name" value="OS03G0233200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00314">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002703">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00347">
        <property type="entry name" value="THAUMATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00205">
        <property type="entry name" value="THN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49870">
        <property type="entry name" value="Osmotin, thaumatin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00316">
        <property type="entry name" value="THAUMATIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51367">
        <property type="entry name" value="THAUMATIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041946999" description="Thaumatin-like protein" evidence="4">
        <location>
            <begin position="26"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="35"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="83"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="98"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="149"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="154"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="162"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="187"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="197"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR002703-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7755014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="258" mass="27231" checksum="630D4864949718E2" modified="2024-05-29" version="1">MLSISLSTYGFTVLIYFLLLSACSSTPVFTIANNCPFTIWPATLSGAGSTPLPTSGFQLNPGQSAQIPTTPKWSGRVWARTGCTFDPSGVGKCETADCGGKMECSGMGATPPASLFEITFGGYNNLDYYDVSXXXXPLIVVPRSTTTGCNATGCASDINMGCPKELQVVGGDDGRGAGGVIACNSACGAFGNDQYCCSGQYANPNTCKPSYYSSIFKRACPLAYSYAFDDGTSTFTCNAYEYLIIFCPNNNNNNNNNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DA75</accession>
    <name>A0AAD5DA75_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_031864</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000632">
        <property type="protein sequence ID" value="KAI7756029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DA75"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="141"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="156"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7756029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="193" mass="22490" checksum="E6A01566382E5C11" modified="2024-05-29" version="1" fragment="single">RETFLSLAKSYRQRKVSYVSTTIWGNNRRLDNGVVVEELSHGRGRERERRRRESGVFSDESVVPIHGLKHGEPYGGDRLSDRESVRFCEDERRLRRFFEEYQRHQINLCLRRPLILWIRELEYANIVVHFSDLKKDVKGATTPTPLTPVPTNPFINEKKRDATTRRQLFETEGNKNPAKGTATDNRHAATKTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DAD3</accession>
    <name>A0AAD5DAD3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_015516</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000605">
        <property type="protein sequence ID" value="KAI7756077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DAD3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47908">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47908:SF2">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT (TPR)-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7756077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="230" mass="26064" checksum="2D3EF979E93FFD61" modified="2024-05-29" version="1">MAAQRIITPITTNTSNPLLHSPSTFPITHHSFPTTTTIFTPHILPRNTKLSTSVSGIWDAITGGPDSSRQALIAIRRGMVLFRQRGLSLYYLDRFEEGAEQFRIDVAQNPNDTEESIWCFLCEAQLYGAAEARKQFLEVGRDPRPVMREAYNMFKDGGDPEKLVDMFSKGRESEYFYASLYAGLYYESQNIADKAKVHIVASCKSPYGERSDDYMASLAKVHCICRNWEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DAS0</accession>
    <name>A0AAD5DAS0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_030162</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Tom40 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010001292">
        <property type="protein sequence ID" value="KAI7755316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DAS0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.10">
        <property type="entry name" value="Porin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023614">
        <property type="entry name" value="Porin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027246">
        <property type="entry name" value="Porin_Euk/Tom40"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037930">
        <property type="entry name" value="Tom40"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10802">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01459">
        <property type="entry name" value="Porin_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1134">Transmembrane beta strand</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7755316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="222" mass="25247" checksum="D64F8D6A10B1A196" modified="2024-05-29" version="1">MATREAVKPEKTDYMKLPCPVPYEEINREAFMSLNAEYFEGMRVDFTKRLNQRFSLSHSITMGPTEIPCQSTETIKIPTAHYDFGADFMDPKLMLRGRVMTDGRVDAALTSEPQTSRGMFNIDYKIANTGNVALSYVQKVSEKVSLVSDLMYNHISRDVIASFGYDYILRQCRVRGKIDSNGCTSAFVEESLNEGLKFVLSAELDHKKKDYRFGFGFTVGEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DBT2</accession>
    <name>A0AAD5DBT2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">AMP-dependent synthetase/ligase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_023609</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a long-chain fatty acid + ATP + CoA = a long-chain fatty acyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15421"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57560"/>
            <dbReference type="ChEBI" id="CHEBI:83139"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Phytoalexin biosynthesis; 3,4',5-trihydroxystilbene biosynthesis; 3,4',5-trihydroxystilbene from trans-4-coumarate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000768">
        <property type="protein sequence ID" value="KAI7755841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DBT2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009698">
        <property type="term" value="P:phenylpropanoid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272:SF83">
        <property type="entry name" value="ACYL-COA SYNTHETASE LONG-CHAIN, ISOFORM J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0587">Phenylpropanoid metabolism</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042015370" description="AMP-dependent synthetase/ligase domain-containing protein" evidence="8">
        <location>
            <begin position="18"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="9">
        <location>
            <begin position="99"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00036813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7755841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="641" mass="70006" checksum="C4AB273451E1999A" modified="2024-05-29" version="1" fragment="single">MSAYIVGVLAPLLLTLALRNSKKGKNRGLPADVGGDPGYAIRNYRFTSPVETAWEGIKTLADLFEQACNQHADKNLLGTRKIISRETEVNQDGRSFEKLHLGDYEWMTYGQTFQVVCNFASGLMQIGHKTGERVAIFADTREEWFIALQACFRQNVTVVTMYASLGEEAICHSLNETEVTTVICGNKEMKKLIDISGQIDTVERVICMDDEITSNPILVEGSNWKVFSYSEVKKVGRENPVEADLPLPADVAVVMYTSGSTGLPKGVMMTHSNVLATVSAVMTIVPGLGVKDVYLAYLPLAHILELAAENLIAAVGSSIGYGTPLTLTDTSSKIKRGTKGDASALRPTLMASVPAILDRVRDGVRKKVDSAGGLSKTLFNLAYNRRLSAINGSWLGAWGLEKLLWDFLVFRKVRAILGGRIRFILSGGAPLSADTQRFINICLGAPIGQGYGLTETCAGGTFSEYDDTSVGRVGAPLPCSYIKLIDWPEGGYLTSDSPMPRGEIVIGGPNVTLGYFKNDEKTKEVYKVDERGFTWFYTGDIGQFHKDGCLEIIDRKKDIVKLQHGEYVSLGKVEAALSVSPYVDNIMVHANSFHSYCVALVVASQSAVESWALQKGIKFDDFANLCETDETLKEVYGSLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DCA2</accession>
    <name>A0AAD5DCA2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Isopentenyl phosphate kinase</fullName>
            <ecNumber evidence="2">2.7.4.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_014752</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>isopentenyl phosphate + ATP = isopentenyl diphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:33963"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:65078"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.26"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the isopentenyl phosphate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.26" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010000174">
        <property type="protein sequence ID" value="KAI7757154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DCA2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102043">
        <property type="term" value="F:isopentenyl phosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04241">
        <property type="entry name" value="AAK_FomA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="entry name" value="Acetylglutamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024192">
        <property type="entry name" value="Fosfomycin_R_FomA-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040647">
        <property type="entry name" value="IPPK_Arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43654">
        <property type="entry name" value="GLUTAMATE 5-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43654:SF1">
        <property type="entry name" value="ISOPENTENYL PHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016496">
        <property type="entry name" value="Kin_FomA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="Carbamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aspartate/glutamate/uridylate kinase" evidence="11">
        <location>
            <begin position="109"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="246"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="300"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR016496-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7757154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="353" mass="38181" checksum="3C7E32BA058FA72C" modified="2024-05-29" version="1">MELSGHSRKFKMQVVGQMLEVGGGRRLQVVVVGVWRWWLKVAGGAAITCKNEFEKINEANLITVSSQLREAMMFGSSSKSLLEMDWSKRPGVAEAPNNVDDFKEQQVLDYNSFVVIHGAGSFGHFQASKSGVHKGGLSQPLVKAGFVATRISVTSLNLEIVRALARDGIPSVAMSPFSCGWSTRDRNIASADVSTVVEALDSGFVPVLHGDAVLDESLGCTILSGDVIISHLAAHLKPQFVVFLTDVFGVYDRPPAEPNAVLLREIAVREDGSWSVVNPTFKDSDKQVEISVASHDTTGGMVTKISEAAMIAKLGIDVYIVKAATEHSLTALSGKLRDEIPEDWLGTVIRYVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DCV7</accession>
    <name>A0AAD5DCV7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_022282</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000411">
        <property type="protein sequence ID" value="KAI7756396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DCV7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7756396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="59" mass="6496" checksum="C6BB04CD05B80680" modified="2024-05-29" version="1">MQVKKMGLYAASVAIDEATKLIQKQNSESQPDLPPIEQVDEKLEVVVEPKEAVVSEDLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G1S0</accession>
    <name>A0AAD5G1S0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_028979</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012208">
        <property type="protein sequence ID" value="KAI7724498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34835">
        <property type="entry name" value="OS07G0283600 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="718"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="853"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="183"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="208"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="718"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="730"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="863"/>
            <end position="877"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7724498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="935" mass="102522" checksum="EAB859587660AACB" modified="2024-05-29" version="1">MKTLKSKHKGFVTLYPPVLEGFGKMGDFEGHTNDSGIAIVVDDLSPVSDANPSLERFHRLPRQKKFRRKLYFGKGRPILWKRDKKRSARDVVIVSDDSTHGRGVFKDHGPSQSPHTETKRRLEYDSDTTTSTLKRKKIGGIAKETVGPTDASTSRKHKGKESVIVVSSSTRGEKTKARKRATVTESTSGSVRVTRSGSRVVESSTPPDVKKTRTKESRASTAVKRKRSKKEDTQGMGPGKKRKRSKKNEWNGIRTRSSPFQFARCVEVLSEEQRLVVKELGFGRLLNFKMDGIASKLGHYVVDNFNPDTLEMTFPCGRIIIDEKVVERILGIPRGGVPFNSFGRLPKYDVGVAKWRKRYPDNKYVAQKQMVDMIQNESEGADSFEFRLDFLMCFYAVMIECHKQGSLKDSTVEYLRDNMDFAKVDWCGILVDKXXXXTPFAGPLALLTLVYVDCVTCKGIDIDDSVYPMTFWSFQRLRDREKLEIKNGGFGTGGFKGLSACENVHTETDEMSIQEKVMEVDKLVDLLVKTKKRIETSIGKLYDEYPDRADVVAVKAKYESVTEELPKWKNKVGTRDTVSCSIAGVLGDLAEMPEDKDGVKQVASDIPAVQEVASTNPGPATELDVAAGLDDLSTGTQKSPLTTPMQVRNKTVLGNDDVSDASPVDSQYRKILHDVEKRKLVDVVLDIDKAVAGGMSCEKVDPLVPIVDVHNKGDVGKVLEDDKADVDPTNRPSTIDVGTSDQDKDKDASCSTGHAGPPVTADMQSELQKCNPTNENAAADHKSYPSDLSAMASLSTRHAALPVGTDMQTGSTKNEKAAAGPETCPTVLSTAANVQHTQQQTTCVPIHVETQTDPDTVSKETNNEVSEATKLSANAPTGYTAPNPAAHDDVGSSHGPSGKVQASVSMPFDDYDIPSFSVGLTQLDQNDPVISNDAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G2H0</accession>
    <name>A0AAD5G2H0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_026074</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011990">
        <property type="protein sequence ID" value="KAI7725276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G2H0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019748">
        <property type="term" value="P:secondary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF382">
        <property type="entry name" value="PEPTIDASE S10, SERINE CARBOXYPEPTIDASE, ALPHA_BETA HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7725276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="169" mass="19109" checksum="3359ADA01F0C9070" modified="2024-05-29" version="1" fragment="single">HSFFAYIVDASDGQKTDSMLSDCDREQKMSGKGVQLCEISRVVLCWKPWDGHKRPIKRVQIQERDDLVAICVLCWLHVLREGGMAVTTTMVHRILPVTYGISYSGIVIPMITEELYKGNDEGLQPTVNIQGYMAGNPLTDKTGDINSRLEYAYRVALISHELYEATKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G2K7</accession>
    <name>A0AAD5G2K7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Sec-independent protein translocase protein TATC, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_015953</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TatC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011971">
        <property type="protein sequence ID" value="KAI7725383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G2K7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033281">
        <property type="term" value="C:TAT protein transport complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009977">
        <property type="term" value="F:proton motive force dependent protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043953">
        <property type="term" value="P:protein transport by the Tat complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00902">
        <property type="entry name" value="TatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019820">
        <property type="entry name" value="Sec-indep_translocase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002033">
        <property type="entry name" value="TatC"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00945">
        <property type="entry name" value="tatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30371">
        <property type="entry name" value="SEC-INDEPENDENT PROTEIN TRANSLOCASE PROTEIN TATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30371:SF0">
        <property type="entry name" value="SEC-INDEPENDENT PROTEIN TRANSLOCASE PROTEIN TATC, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00902">
        <property type="entry name" value="TatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01840">
        <property type="entry name" value="TATCFAMILY"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01218">
        <property type="entry name" value="TATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="137"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="188"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="220"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="268"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="304"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="328"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7725383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="353" mass="38895" checksum="1B0BC552AD331049" modified="2024-05-29" version="1">MGSTNTYTLIHNLNLNTTRSFNCMNSTINHSNSLQINNRKHPLSFSCRRQRKINKVVCFALEDVRDMQTRLGIGGSGPVVEDRPEVVDDIQEDALANTDSSANGSALYNFLYPDKELLPEDKEMTIFDHLEELRDRIFVSVLAVGAAIMGCFVYSKDLIMILEAPVRTQGVRFLQLAPGEFFFTTIKVSGYCGLLLGSPVILYEIIAFVLPGLTRSERKFLAPIVLGSSILFYTGIAFSYVVLTPAALNFFVSYAEGVVESLWSIDQYFEFVLVLMFSTGLSFQVPVIQLLLGQVGLVSGDQMLSIWRYVVVGAVVAAAIVTPSTDPLTQMLLAGPLLGLYFGGAWTVKLIGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G2R0</accession>
    <name>A0AAD5G2R0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Condensin complex subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_004157</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CND2 (condensin subunit 2) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011811">
        <property type="protein sequence ID" value="KAI7725952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G2R0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000796">
        <property type="term" value="C:condensin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007076">
        <property type="term" value="P:mitotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022816">
        <property type="entry name" value="Condensin_barren_su2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13108">
        <property type="entry name" value="CONDENSIN COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13108:SF9">
        <property type="entry name" value="CONDENSIN COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05786">
        <property type="entry name" value="Cnd2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017126">
        <property type="entry name" value="Condensin_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0226">DNA condensation</keyword>
    <keyword evidence="8" id="KW-0498">Mitosis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="271"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7725952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="611" mass="67921" checksum="A4197CA646EBC856" modified="2024-05-29" version="1">MAELLSPSRTPGFKQRAPVLSPDRTLFLGSNDDKLERAQARAARAAANRRKPVGLQQAKDESASDPCLGQDQIMELLQNCIKLASENASCTLEAGVRIYSMRVDSVHSEAYKVLGGISRVAQDAEQDVVQDGNTDNMQVEGTSKKEKEYKLSPLTTLESSFEALNVKKFDVAFAVDPLYHQTTAQFDEGGSKGLLLNNLGVYGGCRMVFDSLEVPGKCMSLSSDHDKTNTIDISHARDCIEQMVANFVTKLEISPSLKDIVNLFDEDNKRPADTFSSSQKSVEAEPDNEACDDNEFNDGGHENLETWDFINDNQTNVNDECTYEDEDDLFVPAHHEVSQACWDRGVRPVGIGEPLNQSKWVRVKMLSVLVLIEIGLDDPAKETGSPKMPKRNKKQAEPDIEFTKSLDLDNEMSSIFAPPKNPKTLLLPANREPCNTTLPEDCHYQPEDLVKIFLLPNVMCLGKKGRRHSDENGHQGECNNETFPSWDDECGHFDDGNNYNSDVDDSTPLVSQPRQVNKIEVEYDKTSKQVDVQALKEILWSSMQETPETKTLSFKHLLKSFPTDKKKPAAASVDIISPHLCFICVLHLANEHGLSIIGCDDLDELTINLPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G2S6</accession>
    <name>A0AAD5G2S6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_021060</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011793">
        <property type="protein sequence ID" value="KAI7725982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G2S6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024489">
        <property type="entry name" value="Organ_specific_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33731:SF18">
        <property type="entry name" value="ORGAN SPECIFIC PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33731">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10950">
        <property type="entry name" value="Organ_specific"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="138"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="167"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7725982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="190" mass="21617" checksum="F44F3C1F2D52CEEA" modified="2024-05-29" version="1">MTAINIDARPHPEDYWQGSSVGRGTSVSPQPVKQNHCHTMVRARNHISSVNGELEPRPNISVYDNDTGLKGEKNVDEEFEPRPNVSVYDNDTTLKGEKHVDEEFEPRPNISVYDNGTTLKGERHVDEEFEPRPNVSIYDNNTSLKGKKTVNDEFEPRPNISVYDNDTGLKGKKTFDQEFEPRPNVSVYED</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G389</accession>
    <name>A0AAD5G389_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_023229</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DnaX/STICHEL family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011639">
        <property type="protein sequence ID" value="KAI7726537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G389"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009360">
        <property type="term" value="C:DNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005663">
        <property type="term" value="C:DNA replication factor C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003689">
        <property type="term" value="F:DNA clamp loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18137">
        <property type="entry name" value="HLD_clamp_pol_III_gamma_tau"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000013">
        <property type="entry name" value="DNA polymerase III subunit gamma/tau"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000014">
        <property type="entry name" value="DNA polymerase III subunit gamma/tau"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.272.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022754">
        <property type="entry name" value="DNA_pol_III_gamma-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012763">
        <property type="entry name" value="DNA_pol_III_sug/sutau_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050238">
        <property type="entry name" value="DNA_Rep/Repair_Clamp_Loader"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054506">
        <property type="entry name" value="DnaA_N-like_STI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045085">
        <property type="entry name" value="HLD_clamp_pol_III_gamma_tau"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02397">
        <property type="entry name" value="dnaX_nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669:SF63">
        <property type="entry name" value="PROTEIN STICHEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669">
        <property type="entry name" value="REPLICATION FACTOR C / DNA POLYMERASE III GAMMA-TAU SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13177">
        <property type="entry name" value="DNA_pol3_delta2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12169">
        <property type="entry name" value="DNA_pol3_gamma3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23007">
        <property type="entry name" value="DnaA_N-like_STI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22608">
        <property type="entry name" value="DNAX_ATPase_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="post-AAA+ oligomerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="8">
        <location>
            <begin position="459"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="130"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="323"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="780"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="955"/>
            <end position="981"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1007"/>
            <end position="1036"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="327"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="785"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="812"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7726537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="1110" mass="122880" checksum="EC8F52B335569ECD" modified="2024-05-29" version="1" fragment="single">MSATNNNNNNNRSSSYSGDPSNLHLKKELTQIRKAARVLRDPGTTSSWRSPLASARSINTTRYNHHYRNTSSGYSVEDIIGSNNNLYVTGAGSNNNVNNININNNNNNSNSRRNEKKVFLYNWKTQRSESERSVSVRGSRHRDDGEESVDDSLSDARNDALVNLTKKKKVGNSAAALRLELQRQLSLVDEYDSDGIVVKGTSPLLARLKSGKLLRGGSRKDDSSYCYSTPALSTSSINKRWVRNPSTVGSWDATTTGSLIDGMDDEEDQLGLPGRQGCGISCYFSSSKRSTPKRGVCGTCYSPSFSDTLRRKGSSMLCGSGSGTRLNYHRRQHRSSRGGYNNKRVNHKTSHVPLLTNGVESGDPSCSSTGTDDDLSTNYGELDLEALSRLDGRRWSTSHKSQQGLELVAVNGEIEVDSPSSLDNVNCFSYKYKPMFFEDIVGQNVVVQSLVNSVIKGRIAPIYLFQGPRGTGKTSTARIFAAALNCLATGDTRPCGVCRECAEFISGKSWVITEMDGSSKKGIDKVRFLLKKLQTGSSTSTFIRHEVYVIDECHLLPSKLWLAFQKFLEEPPRNVVFIFITTDLDNVPRAVLSRCQKYSFNKIKDSDIVNRLRKIIQEENLDVESDALDLIGLNVDGSLRDAETMLDQLSLLGKRITADLVNELVGVVSDDKLLELLELAMSSNTAETVKKARELMDLGVDPMVLMSQMATLIMDIIAGTYQVVEARYGDSLFGGRNLTDGEVERLKQALKLLSEAEKQLRVSSERSTWFTATLLQLGSVQSADPTPSGSSRRQSSRTTDDDPSATFKDTYFQKQKSTPVKPLNSRNSSHSTPQDALQSMRQLINDDGPSVSRHDDVMHGNTSSNILDEIWARCIDKCHSKTLRQLLHTYGNLVSISEDKDTVVAYIVFRDKDIKSRVERFLSSITNSFEIVLRRNIEVKIILLSEDGPTANKGEPVVPTNGSTPIDNFSDPGSLKEGRKGGNPIQRIESIIHEQRLETAWLQTTEKGTPGSLNRLKPERNQVLPQDGGSSGQMETSQQQWEDELTHELNLLKINDRKPFAKENTTISPSLLHDANLVGKYSKDSMGYESGSGAGGCFCFNKTKRNRRGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G4H2</accession>
    <name>A0AAD5G4H2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_027653</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011214">
        <property type="protein sequence ID" value="KAI7728440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G4H2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080188">
        <property type="term" value="P:gene silencing by siRNA-directed DNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044567">
        <property type="entry name" value="CLSY/DRD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45821:SF1">
        <property type="entry name" value="ATP-DEPENDENT HELICASE FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45821">
        <property type="entry name" value="SNF2 DOMAIN-CONTAINING PROTEIN CLASSY 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="182"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="9"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="186"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="220"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7728440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="496" mass="54763" checksum="683440319482BCE8" modified="2024-05-29" version="1" fragment="single">MDIEDLMNTPYTTSSTPSGTLKGQLINTEESTFPSPVYTAYAFDAKKAKRTVDHTNPFAIRDWMQELDSAKYGSVTKDIEDLILLRKQMLMPSFRRFPSLAADLMETNPVKNEVATTNPVRDIVMHDAGNVHDTNAPGTVVTIESDDDEPAIGRPFQPYRDVVLNKPEGQFSLKQFLEGDIFQGQSSGGRGIGGGGGGGGGGGGGGGSGGGRGRGRGRGKGREKGVEEKQTNIEDKNVDGDADVERKHDKGEYVGGDSDSDMEGSDPHSDANMDGLADIWQEMNFGLESSKDDMDHSVTEHAKVDEECDHSYILKDDIGYVCRVCGVVERSIDTIIEFQRPKSSKSIRTYYNEGRSDKSGEASGAVLDGVKTPGKDFSVVEVSAHPRHKKQMKPHQIEGFNFLLTSPEEKDHTTCFRKESIAKMWFEWNEYCGRNEFEMETIDIKDCGDEFLQTPWLNEDVAALSRRAETGESNGLGNGSRWLWLETRSRIEWVGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G4P5</accession>
    <name>A0AAD5G4P5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PPIase cyclophilin-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_001356</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclophilin-type PPIase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011389">
        <property type="protein sequence ID" value="KAI7727378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G4P5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016018">
        <property type="term" value="F:cyclosporin A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071:SF561">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="3">
        <location>
            <begin position="9"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="213"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="215"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="237"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="265"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="283"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="300"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="348"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="364"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="386"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="433"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="445"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="487"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="536"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="595"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="688"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="738"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="758"/>
            <end position="776"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7727378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="802" mass="89928" checksum="62461AC5C866F34B" modified="2024-05-29" version="1">MSKKNPLVYFDVSIDGDPIERMVFELFSDTVPKTAENFRALCTGEKGNSQKTGRPLHYKGSFFHRIIKGSLAQGGDFFKRDGSYGESIYGGKFPGENFICSRLVGTQCGGRRVPCNVNKTTTNSGTWCDESSKLKHDAPGLLTMAISDRDSRGSLFNITFAANRHLDRKYVIFGKLVQGVDVLKKIENVGDEDGRPTVTVKIIYCGEISESENQSSDKKAASKKAGKDVSSEVHSHEVRRKGKHKKSSKDRRKKRKRYSSTESDSSSDVDSESSDSDSEFDSDISLSSSSAISSSSDDRRKKKKRSSRKDRHKRSKRRDKRRDKKRRKRDKKSKRKSKRASDSTSESESEHGSEDNGDVRKPNQKLNNSAKITDGNQSPLAEVEDGSVRHKTGDAYEREDGEYPKENGDRPSNGVDVKVAERPNVASDHPHNSRSISRSPQRALSKSMSISPRGSPSVSPRRNKSRSPSVSGSPRRDTLENTRSPVRSESSKSPARSISRSPMRGKNIRNLSPSPVRSRIQKSVSRSPTPSPSPPRSRKSLRKSVSRSPVRSSRRSYSRSPVRSSKRSLSRSPVRSSKRSLSRSSGRAPLRRSLSRSPIRPPVRGSRRSYSRSPVGGRRGRSPSRSVSPDGSPKRIRRGRGFSNRYSYARRYHSPDRSPVRSYRFGRSDRDRYSSYRRRSPLRDRSPPRYRARRSRTRSPSVSRSPVRYRRRYSRSRSPVETSRYRPSPPIERRRGPRSVTPPSRSRSPSRSPSPPRRSVRSRSKSKSKSISKSRSRSPSGSPAGKGGLVSYGDGSPDSGQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G4R7</accession>
    <name>A0AAD5G4R7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_019826</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011238">
        <property type="protein sequence ID" value="KAI7728307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G4R7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7728307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="86" mass="9977" checksum="EE821E875BA665D4" modified="2024-05-29" version="1" fragment="single">MNSKVYLDKSNALDEMRDSPGESESDDDDDDDDGLDFGKDDEEGQQTMELEKMRMDFQRDLELQKKQILERAQAEIAKIREGDDED</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G5A6</accession>
    <name>A0AAD5G5A6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GMP synthase [glutamine-hydrolyzing]</fullName>
            <ecNumber evidence="2">6.3.5.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">GMP synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Glutamine amidotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">M8C21_030630</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">AA19_3_7</strain>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the conversion of xanthine monophosphate (XMP) to GMP in the presence of glutamine and ATP through an adenyl-XMP intermediate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>XMP + L-glutamine + ATP + H2O = GMP + L-glutamate + AMP + diphosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:11680"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.5.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.2" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010011274">
        <property type="protein sequence ID" value="KAI7728081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G5A6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003921">
        <property type="term" value="F:GMP synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01742">
        <property type="entry name" value="GATase1_GMP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01997">
        <property type="entry name" value="GMP_synthase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.10:FF:000002">
        <property type="entry name" value="GMP synthase [glutamine-hydrolyzing]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000001">
        <property type="entry name" value="GMP synthase [glutamine-hydrolyzing]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000001">
        <property type="entry name" value="GMP synthase [glutamine-hydrolyzing]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00344">
        <property type="entry name" value="GMP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001674">
        <property type="entry name" value="GMP_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004739">
        <property type="entry name" value="GMP_synth_GATase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022955">
        <property type="entry name" value="GMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025777">
        <property type="entry name" value="GMPS_ATP_PPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00884">
        <property type="entry name" value="guaA_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00888">
        <property type="entry name" value="guaA_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000848">
        <property type="entry name" value="PRK00074.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11922:SF2">
        <property type="entry name" value="GMP SYNTHASE [GLUTAMINE-HYDROLYZING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11922">
        <property type="entry name" value="GMP SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00958">
        <property type="entry name" value="GMP_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00096">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54810">
        <property type="entry name" value="GMP synthetase C-terminal dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51553">
        <property type="entry name" value="GMPS_ATP_PPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="6 14" id="KW-0332">GMP biosynthesis</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 14" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GMPS ATP-PPase" evidence="15">
        <location>
            <begin position="234"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="262"/>
            <end position="268"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00044933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAI7728081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="561" mass="61935" checksum="CC7B67370453DD2B" modified="2024-05-29" version="1" fragment="single">PPTVKNPNPIYTSHLHRTNHPSMEAQAVKSNLVLILDYGSQYTHLITRRIRSLSIFSLCISGTSSLKSITDLNPSVIILSGGPHSVHAPHAPHAPSFPDGFIEYVEKNGIFVLGICYGLQLIVQKLGGVVGVGEKQEYGRMVVEVVKECGEGLFMGKKVGDKQVVWMSHGDEAVVLPGGFEVVARSEQGGIAAVQDSGRRFYGLQYHPEVTHSPEGMELLRHFLFDICGVTAGWKMEDVMEEEIKVIKSMVGPDDHVICALSGGVDSTVAATLVHKAIGDRLHCIFVDNGLLRYKEQDRVMGTFEKDLHLPVTCVDASVQFLSELKGVTDPEKKRKIIGKEFISVFDAFSHDLEKKLGRKPTYLVQGTLYPDVIESCPPPGSGRTHSHTIKSHHNVGGLPKDMKLKLIEPLKLLFKDEVRELGKILDVPVGFLKRHPFPGPGLAVRIPGDVTQGNALDILRQVDEIFIQAIKDAGIYDEIWQAFAVFLPIKTTGVQGDQRTHSNAVALRAVTSQDGMTADWYYFEHKFLDDVSRKICNSVRGVNRVCLDITSKPPSTIEWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G5W3</accession>
    <name>A0AAD5G5W3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PRONE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_010232</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011169">
        <property type="protein sequence ID" value="KAI7728666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G5W3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.2010:FF:000001">
        <property type="entry name" value="Rop guanine nucleotide exchange factor 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.2010:FF:000003">
        <property type="entry name" value="Rop guanine nucleotide exchange factor 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2010">
        <property type="entry name" value="PRONE domain, subdomain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005512">
        <property type="entry name" value="PRONE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038937">
        <property type="entry name" value="RopGEF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33101:SF80">
        <property type="entry name" value="RHO GUANYL-NUCLEOTIDE EXCHANGE FACTOR 14-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33101">
        <property type="entry name" value="ROP GUANINE NUCLEOTIDE EXCHANGE FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03759">
        <property type="entry name" value="PRONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51334">
        <property type="entry name" value="PRONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRONE" evidence="3">
        <location>
            <begin position="112"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7728666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="550" mass="61361" checksum="7378586A489CE0BA" modified="2024-05-29" version="1">MVLLRRKLACCTWNRDINNSIDFNQPSPNGIMTYNGLESCILSANSCEDESFTSTGDECPTDSPDEDASNCSCVNDVCGSVSSHDSMMMVKSDDQKADAWEYSETGQQCTTKDEPLSQSCHEEIMKETFAKLLLGEDTTGGRKGHSSALALSNSITKLARSVFGELWKLEPLLEETKNRWRREMQWLLSPTKYMVELVPAKQCGANGRALEASIPSLLKLIMRPKARADVHMNLPALQKLDSLLLDTLDAMKSTEFWYEEGSRLGESKRVEEGKKWWLPLPRVPEGGLSDGERKGLLNWAKLVHQIFKAAKSINESVLAEMPIPKIIGEALPKSGKANLGDDLYRTLNAASSSRMLSSLNIMSEHSALEIINKLEATIYSWKERITIDTTGTKCQARTSWSLKGPLKLNKIKFLINRAEVLMQQIRNRYPNLPQTFLDVMKIQYGKDIAHAILEAYSRVLGNLAFTVLTRIEDICQEDVSADPNSPLATNSHPGVSGIFMSSVSARYTLFDKLNYKDGRLSLLEAEKASYTSFLGVETNTISVPSLMLYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G8G4</accession>
    <name>A0AAD5G8G4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PPIase cyclophilin-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_027135</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010482">
        <property type="protein sequence ID" value="KAI7731321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G8G4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000040">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044185">
        <property type="entry name" value="CYP26-2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47724">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE CYP26-2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47724:SF1">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE CYP26-2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="2">
        <location>
            <begin position="222"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="178"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="197"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7731321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="442" mass="47595" checksum="747F11E3CA6FA8B2" modified="2024-05-29" version="1" fragment="single">LSLSLSQHTNTYTRQKPLMEKILQNLNILHSTAEHLHRQQPPPPTQIQNIPTSPAAIKLSRRQLATCTKSSLLLILTSQFQDHFLLPNARAQEQNLLAPEDPPQSLENDIPIENNTTASPADENAVDTSGTNTPTKENPMETSSTNGPIKESATENSGTDGPTEENPPDASATANPIEENSAETSATASTTTEENQADSSSDTSNTTEVDSCIDKVVTKRAFLDIAIDGKPVGRIVIGLHGNNAPNGTTKFSDFVSGAAGISYRRKEFVKITPSYIQHGGVRSYGVDAELAATTGRNFAVDNLVAELESENERCPGTKNVAGSVGIIVRDPFKPPPKQKLVARNGKLVIDEEEIGKEPNGTEFVISTKDSPELDASTLVIGKVLEGMEVVEKIGQVKTVQENTSSPYFRVAKLIGDKRAVVAERGFNRPYSKVIVTNCGIMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G8Z3</accession>
    <name>A0AAD5G8Z3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_000941</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009939">
        <property type="protein sequence ID" value="KAI7733585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G8Z3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36772">
        <property type="entry name" value="SERINE/THREONINE-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36772:SF1">
        <property type="entry name" value="SERINE_THREONINE-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="109"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="174"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="49"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="176"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="196"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7733585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="211" mass="24611" checksum="2A9E3A05D9C89531" modified="2024-05-29" version="1">MHIIAHLFMQDPKNNSATSRKPWYQRAMEIASLWKTTNPKSPEIPTPNPTLKKNLSTSTKYTTTRNTTPNREKLRKSTSLKVATSFTRVCLCVPISSYTEVFQAEVPPRRSNTYPRSKPFPTCIPERPPPRLSIGGGRRIFRGKSLTDDVLMRRFVIEEEAMMQERRRNEMEVIRKRNAKRRRRLGPSPLSRMVLAEEEEEDEYEDSRSEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GBM1</accession>
    <name>A0AAD5GBM1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">PGG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_017877</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009476">
        <property type="protein sequence ID" value="KAI7735519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GBM1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026961">
        <property type="entry name" value="PGG_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24186:SF46">
        <property type="entry name" value="PROTEIN ACCELERATED CELL DEATH 6-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24186">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13962">
        <property type="entry name" value="PGG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="502"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="543"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="82"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="122"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="332"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="PGG" evidence="9">
        <location>
            <begin position="497"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7735519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="574" mass="63444" checksum="8D4CCA4E60A164AF" modified="2024-05-29" version="1" fragment="single">MDTRHNNHLMKLELFKAAKKGCLKDFVAKYNQLQSLYQPISLLCTSHRNDTLLHIVAVFGHDRLAKFLAGESPDLITCKNFIGDTPLHVAARAGKLCVLESLLTMQNGTDPPSNYLLAKNKDGNTPLHEAVIHNRNTVVEFLLGKKQDYNNFVSNVNEKGKSPLYLAVESRNARIVSNILNRSGGTTSQNGTNEDVNANVSILHAAILDKITDSSKRIASTSQTRVDEDVETKKCVLHGALTGTDTEGKCAVVENGNVQNSKHVETTKSVLHAAVAGKDIGILRMVAEKVVEVLRDSSNHPLHLAASLGFLDGVNYLLDPKFGMDVLRHDDDGFCPIHLASKNGHLRVTKVLLDHYPDAREFLSKQHQNILHVAAENGKYNIVKYILKTKWAEFLINEKDKDGYTPLHLATINWHPKVVSALTWDPRIDLKQVNDEGLTALEVAERYMEVMAPFKERLTWIALRSAGTPRTHVKDPETVKRLEAAKYEPYNMAYCKGRVNTLLLVSALVATVTFAAGFTMPGGYNNSGDLNLMLNALRKAQRLLGLSLAMMSLAFLAGVNLVVTESYSLSVIIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GBR6</accession>
    <name>A0AAD5GBR6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_024125</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009330">
        <property type="protein sequence ID" value="KAI7736212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GBR6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012862">
        <property type="entry name" value="DUF1635"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33431:SF22">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33431">
        <property type="entry name" value="ENABLED-LIKE PROTEIN (DUF1635)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07795">
        <property type="entry name" value="DUF1635"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="52"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7736212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="211" mass="23989" checksum="99F2013C6DF1F851" modified="2024-05-29" version="1">MDDRSNCILDWPYLSREKSIEELKQSLWLTTMELEATRIRVQEEIRARDDQLTQLKSLLNEAINERNEAQNKCQSLILDKLLLQHNNSTTSFEDEPITNCGFSSSDCEESIVSSPSIETAVQLPAQGKFLDAVMKAGPLLQNLLLAGPLPHWRHPPPQLDTYRIPSPLPRLAIATRSIKYANNCFEVNKKRGFSEDSISSTETKYQRVSLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GC40</accession>
    <name>A0AAD5GC40_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DYW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_005403</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. PCMP-H subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009150">
        <property type="protein sequence ID" value="KAI7736990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GC40"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000366">
        <property type="entry name" value="Pentatricopeptide (PPR) repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000381">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000196">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein At4g14850"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000073">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000031">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein mitochondrial"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032867">
        <property type="entry name" value="DYW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046849">
        <property type="entry name" value="E2_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24015">
        <property type="entry name" value="OS07G0578800 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24015:SF2038">
        <property type="entry name" value="REPEAT-CONTAINING PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14432">
        <property type="entry name" value="DYW_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20430">
        <property type="entry name" value="Eplus_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="210"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="280"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="311"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="412"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="513"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="614"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="715"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="domain" description="DYW" evidence="4">
        <location>
            <begin position="930"/>
            <end position="1022"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7736990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="1022" mass="113812" checksum="CEB3B86FB9ACEFDC" modified="2024-05-29" version="1">MILLRASFFNTPFQSFPLKSSTNLPKYLCRKLTGLRSAASFFINEEEDEGVNNNNNNNSGQIYANHQTYCWLLEGYLSSGSLTDAKKLHGRILKAGFDRDNAIRSRLAEVYVAQGDLGYACQGLGVSVLGLLSKMLADNVDPDEVTFANVLRALSGDRIDYHELKQIHAKIVRHGFGTNYVVCNPLIDLYAKKGFIDTARNVFQGLTMRDNVTWVAMISGLSQNGHEEESILLFCKMHDSGILPTPYVFSSVISSCTKISSFKVGEQLHALIHKWGMSSETFVCNALVTLYSRCGDLVSAEQIFSKMEHRDGVSYNTLISGLAQKGLSEKALQLYESMQSDMLKPDSVTVASLLSACASIGAFSKGLQLHSYAIKAGMCADIIIEGSLLDLYVKCSDVKTAHDFFLTTETENVVLWNVMLVAYGQLGDLNESLKIFSQMQINGMRPNQYTYPSILRTCTVVGALDLGEQIHSQVVKTGFQLNVYVCSVLIDMYSKHGDLYTAQKILSRLNEKDVVSWTAMIAGYAQHDLFNEALKTFEEMIHKGIQSDNIGFSSALSACAGIQAIHQGRQIHAQSVVCGYSSDLSIGNALVCLYARCGLITEAYLAFEKIHVKDNVSWNALVSGFAQSGNYEESLKVFSKMNEFGVEANLFTYGSAVSAAANTTNITQGKQIHGRMIKTGYNLEPEASNVLITLYSKCGSLDDAKSEFLEMTDKNQISWNAMITGYSQHGCGDEAIKLFEEMKQSGCTPNYVTFVGVLTTCSHVRLVDKGLEYFKSMSQDYGLVPRAEHYACVVDILGRAGLLTRALEFIKSMPIEPDSMVWRTLLSACIVHKNKEVGEISAKHLLELEPEDSATYVLLSNMYAVSGKWDDRNRTRKLMKNRGVKKVPGQSWIEIKNTVHAFFVGDRLHPLADEIYEYLDVINKRAAEIGYVQDRYSLLNDLEQEQKDPSTCIHSEKLAITFGLLTLSNNIPLRVMKNLRVCNDCHNWIKFVSKICNREIIVRDSYRFHHFGGGICSCKDHW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GDY2</accession>
    <name>A0AAD5GDY2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cysteine proteinase inhibitor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_021074</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the cystatin family. Phytocystatin subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008720">
        <property type="protein sequence ID" value="KAI7738777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GDY2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004869">
        <property type="term" value="F:cysteine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00042">
        <property type="entry name" value="CY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027214">
        <property type="entry name" value="Cystatin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000010">
        <property type="entry name" value="Cystatin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046350">
        <property type="entry name" value="Cystatin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018073">
        <property type="entry name" value="Prot_inh_cystat_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11413">
        <property type="entry name" value="CYSTATIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11413:SF103">
        <property type="entry name" value="CYSTEINE PROTEINASE INHIBITOR 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16845">
        <property type="entry name" value="SQAPI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00043">
        <property type="entry name" value="CY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54403">
        <property type="entry name" value="Cystatin/monellin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00287">
        <property type="entry name" value="CYSTATIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0789">Thiol protease inhibitor</keyword>
    <feature type="domain" description="Cystatin" evidence="4">
        <location>
            <begin position="12"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU362130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7738777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="211" mass="23875" checksum="26F8F2A8FB5078FC" modified="2024-05-29" version="1">MLRVNLRDEQLTTLGVLKNSSSSNSTDIDSLARFAVEEHNKKQNALLKLARVIKAQEQVVAGTLHHLTLEIEDAGNKKIYQAKVLVKPWMNFKELQEFTHVSEPAINTPSADHDVQKDGDGSGYQSVPVHDPVVQDAANHILKTMQQRSNSLYPYELHEVVHAKSENVEGLAKYDILVKLKRNDKEEKFKANVHKDDKGVFHLNHIVQDRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GE18</accession>
    <name>A0AAD5GE18_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">mitogen-activated protein kinase kinase kinase</fullName>
            <ecNumber evidence="2">2.7.11.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_011111</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.25"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.25"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.25" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010009149">
        <property type="protein sequence ID" value="KAI7737006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GE18"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004709">
        <property type="term" value="F:MAP kinase kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050538">
        <property type="entry name" value="MAP_kinase_kinase_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48016">
        <property type="entry name" value="MAP KINASE KINASE KINASE SSK2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48016:SF8">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="252"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="31"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="85"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="118"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="131"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="193"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="281"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7737006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="333" mass="35287" checksum="C7E0B987A0A2C213" modified="2024-05-29" version="1">MKSKFIEKNMPAWFGRKSTKDKDNPHKNHFHNNNINNHQDNPFYSSNYNPSDRKNKTSFDDGLLSGPTGSPRVSRDFSGPSSGQAVGGGHSGSGFNGFELDRVGHRLPQPGRVLDQGNGSGSISGSGLSSGGSSVSSGDEQSGGDHGQLGVFKGHGDTKLSPQPRSPGPGSKVGTAATSPFHQRLACTSLDSPNRKLDDGKNAGHPLPLPPGSPTSSSSALNCCSPTSPSSVLSNMKTLSVTDNSSCQPSKWKKGRLLGRGTFGHVYLGFNSENGQMCAIKEVKVVADDPSSKECLKQLNQEIALLSQLSHPNIVQYYGSELRYKRGKHISGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GF49</accession>
    <name>A0AAD5GF49_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">M8C21_026372</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="23">AA19_3_7</strain>
            <tissue evidence="23">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010008514">
        <property type="protein sequence ID" value="KAI7740095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GF49"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000060">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000330">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase At4g03230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.90.10.10">
        <property type="entry name" value="Bulb-type lectin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001480">
        <property type="entry name" value="Bulb-type_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036426">
        <property type="entry name" value="Bulb-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024171">
        <property type="entry name" value="SRK-like_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002">
        <property type="entry name" value="RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE SD1-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002:SF548">
        <property type="entry name" value="RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01453">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08276">
        <property type="entry name" value="PAN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000641">
        <property type="entry name" value="SRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00108">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51110">
        <property type="entry name" value="alpha-D-mannose-specific plant lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50927">
        <property type="entry name" value="BULB_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50948">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0430">Lectin</keyword>
    <keyword evidence="13 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-0675">Receptor</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="386"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="domain" description="Bulb-type lectin" evidence="21">
        <location>
            <begin position="10"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="22">
        <location>
            <begin position="290"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="20">
        <location>
            <begin position="466"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="23">
        <location>
            <position position="784"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="KAI7740095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="784" mass="88578" checksum="002E474FFD9F2C4F" modified="2024-05-29" version="1" fragment="single">INNNIPATSVSTLKLDDQLNYTSQLVSPSNNFTLGFFTIPATNYTYLGIWYTNDEKSRRVWVANPSTPITSSSAVLMIDPYLGQMIVATRGGTPIVNVSHNNNNNNMAGQITNLTATLQDTGDFQLKNETDNRIIWRSFDYPTNVLLPGMKLGSDIKTGRNWNLTSWLSDEVPDYGAFSMSWISVGENSQRLLVQRRGRPYWTSGDLRDQTFEFMNVNSLLLSRYNFHYVYNDEERYFSYDSVNNIHPMWILRPHGRIVDGNDRVLSSNDLCYGYDLGNGCVASSDTPKCRSERDKFSPMSGDFVSEVTRRLVDDNSSLSIGDCMVKCWNDCECLGFVNVGNGTGCIRWTGAKSVGNFSVDSQGNAVSKFVLVSTNADKGNDAKTLIWAPVVAGICLILFCFGLLWYVKKRKVKREERQKRDERRFLEQMAPESFRDSSDIERDGRKGSDMVLFSFAAIAAATNDFADQNKLGKGGFGPVYKGKLSNEREIAVKRLSRTSGQGLEEFKNELILIAKLQHTNLVRVLGCCIHGEEKMLIYEYMPNKSLDYFLFDGTRKPLLDWPKRWNIIEGIAQGLLYLHKYSRMRVIHRDLKASNVLLDENMNPKIADFGMARIFKQDETEAITKRVVGTYGYMSPEYAMQGTFSVKSDVFSFGVLILEIVSGRRNSSFSHIDKTTNLVRYAWELWQQGDAAKLQDPTLGDSCVVYQLLRTIHVALLCVQENAEDRPVMSDVISMLANGTMQLTAPKRSAFFFGRTASMSTSLERKSKDYSINNMSVTEMEAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GFB1</accession>
    <name>A0AAD5GFB1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Calcineurin-like phosphoesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_004930</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008917">
        <property type="protein sequence ID" value="KAI7737968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GFB1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033308">
        <property type="entry name" value="PGAP5/Cdc1/Ted1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13315">
        <property type="entry name" value="METALLO PHOSPHOESTERASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13315:SF4">
        <property type="entry name" value="METALLOPHOSPHOESTERASE, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="180"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="316"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7737968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="345" mass="38528" checksum="CE78DD3FB1811543" modified="2024-05-29" version="1" fragment="single">VISRYEKVFGSRNYHLRAGEVEFVAVDAQTMDGHPQQKETSASWTFVNNVSRDSLSPPTVLLTHIPLYRSDWTSCGSKRSSPIINQRISQASGDHEITYQNYLTEDTTNKLLDSIKPVLVLSGHDHDQCTVTHTAKHGSVVEQTLGTISWQQGNLYPSFMLLTASKLAVSNATDAVSTQMYIVLFVVTLVVILVWPTNGLNIHLHIGENMRNILGFFKSNTKEKDEDENCEYEMVWDAEGSMHLIKKPKKIVPSHSTERVERGNATLRSAAKKQTAQEVNLLIPQDASGQLGPDAAARLGQVKANKSNVRFVIRRLVRVFQVLSVVAAVNVPIYIMLLFADWIDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GFE2</accession>
    <name>A0AAD5GFE2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_010732</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008628">
        <property type="protein sequence ID" value="KAI7739389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GFE2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36410">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36410:SF1">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7739389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="134" mass="14679" checksum="C467A34D25BE18E4" modified="2024-05-29" version="1">MFSIIRSLPIETKPAISNLLASSNNFAEKATPVIRSVKFEQASAGNQEPDNMDKKEEAKEVEKKGDVMSHSFGVAYATRSDDEGFGGTYGGNQSLSHDDEDKIVHGNSPEYDTTQGSEVKEKEKARHQTQQANS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GFM2</accession>
    <name>A0AAD5GFM2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Oberon-like PHD finger domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_027769</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008371">
        <property type="protein sequence ID" value="KAI7740680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GFM2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040029">
        <property type="term" value="P:epigenetic regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010048">
        <property type="term" value="P:vernalization response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032881">
        <property type="entry name" value="Oberon-like_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044514">
        <property type="entry name" value="VIN3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46286:SF1">
        <property type="entry name" value="VIN3-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46286">
        <property type="entry name" value="VIN3-LIKE PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07227">
        <property type="entry name" value="PHD_Oberon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Oberon-like PHD finger" evidence="7">
        <location>
            <begin position="54"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7740680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="145" mass="15792" checksum="619B9B52D083AC93" modified="2024-05-29" version="1">MDKQVQKNQDSKKVSMGPKGQPSKTIKRKREKPERFPPTTEPCSGSGLLYTWTCRNASCKAELSVADTFCKRCTCCICYHYDENKDPSLWLECASDSTSSSCGSSCHIECALLQRKVGAVDLGQGMQLDGSYCCAKCGNVSGILG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GFP9</accession>
    <name>A0AAD5GFP9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PGG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_008436</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008822">
        <property type="protein sequence ID" value="KAI7738373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GFP9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026961">
        <property type="entry name" value="PGG_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24177">
        <property type="entry name" value="CASKIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24177:SF472">
        <property type="entry name" value="PGG DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13962">
        <property type="entry name" value="PGG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="618"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="656"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="701"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="729"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="domain" description="PGG" evidence="3">
        <location>
            <begin position="608"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7738373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="781" mass="88933" checksum="08EC726FE431A4DC" modified="2024-05-29" version="1">MQMPMQMMKEAGPQLISIDEQQPLSHQQQQVNGRAASFANSRRLKSNRRNLPNRSLIAAQREMYFKFCVPLYEASVKCDWKAAKAILDKDPGLVRYSITENGDTTLHVAASVKTSKQVEKFVKNLVDMMKEEDLELVNEKSNTALYLAAAAGNVKTVRIMVEKNRGLLTIPGARGQMMPLYSAALYGNYEVVKYLYENSNELSDDGWNHQNRGWLLEKCVEADMFDVALQIVQKHPKLETGNLLGILARKPDAFSKTKCNVIERTVDSAYALIGLTERVYEKESEALQLLKTVWEDIAKKPRIEIDEILQGSPDSIKQEEKLTSEKVVQTLQLQKLISELLDKLDVETRSIMGGPSNEIKKDTGVDSGKPDQALQLQKLISEHLVNMHVETQNMIKQDKKSVSGKGDHALELQKRISEHIISMHVNTQNIVKRESGKKDQVWYLQKLVSKHITKMCVASTLKETHSSRVLFIAAEMGNTKFLVELIRRYPDLMWKVNDYNQTIFHIAIKDRHEGIYNLLYEIGSMKDLITPLRDADENNMLHLAGKSATKERLADVSGAALQMQRELLWFKEVRNMIPPSYRERRNKDGLTPRELFTEEHKELIVEGEKWMKGTASQCMVVAALIATIVFAAAFTIPGGYSQNYGIPIFYRRPIFVAFVVADAISLFLSSASILTFLSILTSRYAERDFVDSLPRKLMLGLTTLFLSITTMMITFSVSFFILYHKEMKWIPIFISVFAITSVLLYVVLQYHLLADVIRSTYGSKYLFKPGKQLLYYVNPKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GGG5</accession>
    <name>A0AAD5GGG5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_003949</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008627">
        <property type="protein sequence ID" value="KAI7739451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GGG5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14703">
        <property type="entry name" value="bZIP_plant_RF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000009">
        <property type="entry name" value="probable transcription factor PosF21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044759">
        <property type="entry name" value="bZIP_RF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13690:SF152">
        <property type="entry name" value="BASIC-LEUCINE ZIPPER DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13690">
        <property type="entry name" value="TRANSCRIPTION FACTOR POSF21-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="7">
        <location>
            <begin position="190"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="331"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="342"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7739451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="366" mass="42111" checksum="53C51DEFD1598075" modified="2024-05-29" version="1">MSIPLFKGPHVTPESCLLNGNKENAKSEPSRSIMLPTDWLETATESSHFSHDISKMTDNPPKYTGHRRAHSEIITLPDDLSFDSDLGIVGDFNGAEETEEDMFSMYLDMDNQSGSQVEEPYSLEMQTSRTATTPQPENHVHSYYDRHKVKHQHSMSMDCSAEFNPEDRPLLTGAKKSISAAKLAELALVDPKRAKRVWANRQSAARSKERKMRYIADLEWEVQILQTEATSLCAQLTLLQRDVHGLTVENNELRMRLQTMEQHVHLQDAVTDEMKKEIHHLKLLTGQNLTNGVPMVNFPPLFGLNPRFNPNNQAMHTMLTAQQFHQLQIQSRNQHHHPFRPPQLQFQQQQQQHQFQQQQQQQRNLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GHC3</accession>
    <name>A0AAD5GHC3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Auxin-responsive protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_029244</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Aux/IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimers and heterodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Aux/IAA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008208">
        <property type="protein sequence ID" value="KAI7741339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GHC3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009734">
        <property type="term" value="P:auxin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033389">
        <property type="entry name" value="AUX/IAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003311">
        <property type="entry name" value="AUX_IAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053793">
        <property type="entry name" value="PB1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31734">
        <property type="entry name" value="AUXIN-RESPONSIVE PROTEIN IAA17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31734:SF157">
        <property type="entry name" value="AUXIN-RESPONSIVE PROTEIN IAA27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02309">
        <property type="entry name" value="AUX_IAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51745">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0927">Auxin signaling pathway</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0678">Repressor</keyword>
    <keyword evidence="5 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 8" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="PB1" evidence="10">
        <location>
            <begin position="107"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="14"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7741339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="205" mass="22315" checksum="0AD2F066E47FDE4F" modified="2024-05-29" version="1">MSVPLEHDYIGLSDASSLERSSESSNISSENETNNVLNLKATELSLGLPGFSKSCEENATKGGPIIKNFMSGAKRGFSDVVNLDGSSKWALNTGSESDLSKGVNGVFEKNKTSYPDVKPVEDKKKGHCSSNGIKEREGLSESNLKDLLHGSECVLTYEDKDGDWMLVGDVPWEMFIDSCKRLRIMKGSEAIGLAPRSTEKCKNKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GIG7</accession>
    <name>A0AAD5GIG7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">40S ribosomal protein S26</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_023337</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS26 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008187">
        <property type="protein sequence ID" value="KAI7741476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GIG7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1740.20:FF:000002">
        <property type="entry name" value="40S ribosomal protein S26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1740.20">
        <property type="entry name" value="Ribosomal protein S26e"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000892">
        <property type="entry name" value="Ribosomal_eS26"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047864">
        <property type="entry name" value="Ribosomal_eS26_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038551">
        <property type="entry name" value="Ribosomal_eS26_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12538">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12538:SF0">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01283">
        <property type="entry name" value="Ribosomal_S26e"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00733">
        <property type="entry name" value="RIBOSOMAL_S26E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="183"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="185"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7741476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="225" mass="25643" checksum="4594C841DFA2831A" modified="2024-05-29" version="1">MTFKRRNGGRNKHGRGHVKFIRCSNCGKCCPKDKAIKRFLVRNIVEQAAVRDVQEACAFDQYTLPKLMTLLSQVKHPAQPVVLLLFVLEDNHWIKSPQTFKRRNGGRNKHGRGHVKFVRCSNCGKCCPKDKAIKRFMVRNIVEQAAVRDVQEACAFDQYTLPKLYLKVQYCVSCAIHSKVVRVRSRTDRRNREPPKRFSRPRDDAPKPGQAQRPGGAGGPPPVRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GJN6</accession>
    <name>A0AAD5GJN6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_017334</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rho family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007305">
        <property type="protein sequence ID" value="KAI7745272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GJN6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7745272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="129" mass="14281" checksum="9914DB2F10AFD02B" modified="2024-05-29" version="1">MVGTGQEDYNRLRPLSYRGADVFILAFSLISKASYENVSKKWIPELKHYAPGVPIILVGTKLDLRDDKQFFADHPSATPIHSAQGEELRRTIGAPEYNVKGVFDAAIKVVLAPPKSKKKKKGQKACSIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GLJ4</accession>
    <name>A0AAD5GLJ4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_007988</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006771">
        <property type="protein sequence ID" value="KAI7747355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GLJ4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7747355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="48" mass="5436" checksum="EB0BA356A5F46D10" modified="2024-05-29" version="1" fragment="single">WITSFGCSRRAQRNKHIFSAMANSPGVLMLLDQGHQMGQWRCLSSTQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GNA6</accession>
    <name>A0AAD5GNA6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_026239</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006958">
        <property type="protein sequence ID" value="KAI7746501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GNA6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35503:SF2">
        <property type="entry name" value="OS04G0455700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35503">
        <property type="entry name" value="OSJNBA0006M15.15 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="1">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7746501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="161" mass="18096" checksum="6D1C78FBD53E8870" modified="2024-05-29" version="1">MDALKALSSLNFELKIMNAKNIQLANSSGYLFVRCYLSAGNNKRVRVDSREVSSNGDLSWNESFSLECKGANQSIDMITHGTIVLELRWRSNSIALFRRSQLVGRAEVSWRDAFESPNMEIKKWVMMESTKKGLKASSVCVGMKIEVPSSEDILIVSNLRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GPI0</accession>
    <name>A0AAD5GPI0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF1771 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_007933</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006817">
        <property type="protein sequence ID" value="KAI7747208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GPI0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd14279">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056254">
        <property type="entry name" value="At5g58720/SDE5-like_UBA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013899">
        <property type="entry name" value="DUF1771"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47872">
        <property type="entry name" value="NUCLEAR RNA EXPORT FACTOR SDE5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47872:SF1">
        <property type="entry name" value="NUCLEAR RNA EXPORT FACTOR SDE5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08590">
        <property type="entry name" value="DUF1771"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24767">
        <property type="entry name" value="UBA_At5g58720"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01162">
        <property type="entry name" value="DUF1771"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF1771" evidence="2">
        <location>
            <begin position="317"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="97"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7747208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="475" mass="52676" checksum="64A0BA11B4C1591E" modified="2024-05-29" version="1">MTIVKGASSSINLSNADDKNLEFLIEAFGSMISPEDMASAYCQAGYDVNKASDILYSMLGSSSNNDKHISTDDLTSAPASSFGAAVGTVSSGNRADYAKSRNLTNEPATVLPRSKPRTSGVSVGTVSGIIGADYGRVRAKTKKNCETTKPVKLMSNEFPASQIWVEKPQSVNNAPTETMNRDIEMFLLQMLGDGFQLDMEIIREVLDGCGYDVQESMEKLMNISAPTVDLGSATSSTVDQTSIDTRQDLGIEFEEKLQLNSSSRRSNLEKEIFSNLFNVPERSELGLETVPPIRQPRTKKYGLVTGPLEDGVLEHKTPITLREAVMEHWVTMKQYYRAATEAFAKGNYERSQKLVEEGHFYMAKSREADEKSTKILTQTRDDGEVISINLNNYDPKEAVRLLKTQLKSMSGIPSIHYLKVMVGANGENNKPNARKRLISRLLERDAITWTEEDNGHIMSIRIDVINPKRLSFNKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GPX0</accession>
    <name>A0AAD5GPX0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_002905</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006579">
        <property type="protein sequence ID" value="KAI7748196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GPX0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000949">
        <property type="entry name" value="ELM2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46872">
        <property type="entry name" value="DNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46872:SF11">
        <property type="entry name" value="TRANSCRIPTION FACTOR MYB FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01448">
        <property type="entry name" value="ELM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb-like" evidence="3">
        <location>
            <begin position="215"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="342"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="348"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7748196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="414" mass="46619" checksum="47DD3737A9B1DD9D" modified="2024-05-29" version="1">MLQIVMGTKRPFDEELQEFVKHPKHLENGNKSDSFGEEKPCLEPSQTVVVVGECGETSAPLPLVTGITGREEASGPGPIPGFCSSLFSELYEFNLPRRPLVHYDDTYSSLLNCTPRKEVPIGPEYQADIPEFDADSARNYISNNEREESLTGVCVISNQEDETVIPQTDCQCVDYGSIRCVQQHVNEARLNLKQSLVLEKFANLGFDNMGEEVSSNWTEEEEQHFQDIVYSNPVSHGKRFWEVLSSEFPTRTKKELVSYYFNVFIYRRRAVQNRSHLLAIDSDDDEWWGTKRGSIIEEDDDDDLVVVGPFVDHGDHDHDDDHDSSSEDGVIVTRVKENQVMNASSLSDIRDEKVTSENELEKPTAGNCFGSKSESYLHWDPPYSTMGSTKGVDLLPTCSMIEEIFGSSKTSKST</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GQ92</accession>
    <name>A0AAD5GQ92_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Galectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">M8C21_020495</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">AA19_3_7</strain>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Possesses hydroxyproline O-galactosyltransferase activity. Transfers galactose from UDP-galactose to hydroxyproline residues in the arabinogalactan proteins (AGPs). Is specific for AGPs containing non-contiguous peptidyl hydroxyproline residues. Utilizes UDP-galactose solely as sugar donor. The addition of galactose onto the peptidyl hydroxyproline residues in AGP core proteins represents the first committed step in arabinogalactan polysaccharide addition. AGP glycans play essential roles in both vegetative and reproductive plant growth.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyltransferase 31 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005982">
        <property type="protein sequence ID" value="KAI7751090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GQ92"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990714">
        <property type="term" value="F:hydroxyproline O-galactosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010405">
        <property type="term" value="P:arabinogalactan protein metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00070">
        <property type="entry name" value="GLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.50:FF:000005">
        <property type="entry name" value="Hydroxyproline O-galactosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000071">
        <property type="entry name" value="Hydroxyproline O-galactosyltransferase GALT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000147">
        <property type="entry name" value="Hydroxyproline O-galactosyltransferase GALT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001079">
        <property type="entry name" value="Galectin_CRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002659">
        <property type="entry name" value="Glyco_trans_31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214">
        <property type="entry name" value="BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214:SF212">
        <property type="entry name" value="HYDROXYPROLINE O-GALACTOSYLTRANSFERASE GALT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00337">
        <property type="entry name" value="Gal-bind_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01762">
        <property type="entry name" value="Galactosyl_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00908">
        <property type="entry name" value="Gal-bind_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51304">
        <property type="entry name" value="GALECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="13" id="KW-0464">Manganese</keyword>
    <keyword evidence="11 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="domain" description="Galectin" evidence="17">
        <location>
            <begin position="191"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="83"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="83"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00059439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAI7751090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="680" mass="77525" checksum="7B19FAC8B6A6CF0C" modified="2024-05-29" version="1">MKRSRSEIFGLRRVKLPHILFGLAALYLIIVCFKFSVYFETFSSFDDELGAEFIEVSKPLIRSAYHDEISEVLEEDSRLEVKSGDRSRETLEDKSGFDTVSREEDDDGLSPVDLRYGRLTGNVLRQRRRTRDMSGLEKMADEAWSLGLKAWEEVDKYDGKDTELSSVVEGEHEQCPPWVSISGEELTKGGRIMFLPCGLAAGSSITVVGTPHYAHKEYVPQLVRTKGGDLLVLVSQFKVELQGLKSVVGEDPPKILHLNPRLRGDWSHQPVIEHNTCYRMQWGAAQRCDGLPSKGDDDMLVDGFPRCEKWMRNDVVDSKESRTSSWFKRFIGRAQKPEVTWPFPFVEGKLFVLTIRAGVDGFHINAGGRHVTSFPYRTGYTLEDATGLAIKGDVDVHSVFATSLPTSHPSFSPQRVIEMSDKWKAKPWPHHRIQVFIGILSATNHFAERMAIRKTWMQSSAIKSSDVVVRFFVALSPRKEQNAVLKKEAAYFGDVVVLTFMDHYELVVLKTIAICEYAVQNVSASYVMKCDDDTFTRVDTILKELSSVPNQRSLYIGNLNLYHRPLRHGKWAVSFEEWPEAVYPPYANGPGYIISSDIAKFIVSLHAKRKLRIFKMEDVSMGMWVEQFNSTNPVQYSHHGKFCQYGCIDNYYTAHYQSPRQMVCLWDNLARGRARCCNFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GQL8</accession>
    <name>A0AAD5GQL8_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_009897</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006417">
        <property type="protein sequence ID" value="KAI7748991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GQL8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000004">
        <property type="entry name" value="T-complex protein 1 subunit zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7748991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="127" mass="14204" checksum="3E835AB8CDEB7815" modified="2024-05-29" version="1" fragment="single">MKRRAENCHILTCNVSLEYEKSEINAGFFYSNAEQREAMVVAERHSVDERVRKIIALKNKLCDGTEDNFVVINQKGIDPPSLDLLAKAGIVALRRAKRRNMERLVLACGGEAVNSVDDLTPDCLGWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GR43</accession>
    <name>A0AAD5GR43_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Plastocyanin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_023153</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the multicopper oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005717">
        <property type="protein sequence ID" value="KAI7752232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GR43"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Plastocyanin-like" evidence="2">
        <location>
            <begin position="2"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7752232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="67" mass="7665" checksum="301761FB0C30CF2E" modified="2024-05-29" version="1">MSPLGVPQQVILINDKFPGPVINCTSNNNIVVNVFNNLDEPFLLTWNAQKFMGRWDTRDYVSNPAWN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GR64</accession>
    <name>A0AAD5GR64_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_009781</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006171">
        <property type="protein sequence ID" value="KAI7750279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GR64"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7750279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="150" mass="16425" checksum="C81F4A7F0848BBF8" modified="2024-05-29" version="1" fragment="single">FTYSGFPLSITTKATIGAPPSPITSSLSVRPRNKPMTSTTPGTHNPHLSRHLAAPSVHQTTWTTNLEPNHRLAPSVRNRRVITETFHNLDSSSSDSDSEGEKSFAVRELMEKPLVCVFSNPESDCKIAAKPILRTGIYFLFAFGGIECDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GT33</accession>
    <name>A0AAD5GT33_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ATPase AAA-type core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_014879</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010004440">
        <property type="protein sequence ID" value="KAI7754135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GT33"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00267">
        <property type="entry name" value="ABC_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43158:SF12">
        <property type="entry name" value="ABC TRANSPORTER FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43158">
        <property type="entry name" value="SKFA PEPTIDE EXPORT ATP-BINDING PROTEIN SKFE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13304">
        <property type="entry name" value="AAA_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATPase AAA-type core" evidence="4">
        <location>
            <begin position="80"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="192"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7754135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="256" mass="29278" checksum="70320797BA64EA93" modified="2024-05-29" version="1">MVEPDMVRVLGRSAFHDTSLTSSGDLTYLGGEWRREVAFAGFDVAIQMDISAEKMLFGVSGVDPKRRAELIKVLDIDLSWRMHKVSDGQRRRVQICMGLLKEFKVLLLDEITVDLDVLARADLLTFLKKECEERGATIIYATHIFDGLENWPSHIVYVAHGKLQLASPMEEVKKMSNLSLMRTVEQWLRKERDEERKRRKERKANGLPEFDRRVDGTRVTGDPVRVVNNGWAAGRLNSTVAGEENFVYSSNRVIRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GTH1</accession>
    <name>A0AAD5GTH1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_025131</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010002336">
        <property type="protein sequence ID" value="KAI7754872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GTH1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16180">
        <property type="entry name" value="EFh_PEF_Group_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044590">
        <property type="entry name" value="CML48/49/50"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46824">
        <property type="entry name" value="CALCIUM-BINDING PROTEIN CML48-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46824:SF1">
        <property type="entry name" value="CALCIUM-BINDING PROTEIN CML49-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13202">
        <property type="entry name" value="EF-hand_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="52"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="135"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7754872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="240" mass="26580" checksum="80DD56BA901A65FA" modified="2024-05-29" version="1" fragment="single">QPYGAPAQPYGAPPQTKPPKDHNKPSSSPYGAPPQPYGSPFAALLPSTFPPGTDPNVIACFQVADQDGSGIIDDKELQRALSSYNQSFSIRTVHLLMYLFTNTNARRIEIVESCEIGHVAVDACCGPKEFTQVFYSLQNWRANFEKFDRDRSGQIDANELREALLSLGFAVSPVVLDLLVSKFDKTGGKNKAIEYDNFIECCLTVKGLTEKFKEKDKTYSGNATFTYEEFMLTVLPFLIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GTP3</accession>
    <name>A0AAD5GTP3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Alginate lyase 2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_008382</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005762">
        <property type="protein sequence ID" value="KAI7752081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GTP3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014895">
        <property type="entry name" value="Alginate_lyase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33681">
        <property type="entry name" value="BINDING PROTEIN, PUTATIVE, EXPRESSED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33681:SF18">
        <property type="entry name" value="CONCANAVALIN A-LIKE LECTIN_GLUCANASE DOMAIN SUPERFAMILY, ALGINATE LYASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08787">
        <property type="entry name" value="Alginate_lyase2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042277740" description="Alginate lyase 2 domain-containing protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="Alginate lyase 2" evidence="2">
        <location>
            <begin position="42"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="Alginate lyase 2" evidence="2">
        <location>
            <begin position="128"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7752081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="180" mass="20686" checksum="FB3EAE5D0B0ECB4C" modified="2024-05-29" version="1">MKQISFVLGFIFLLGRIIDTCSQSNPTSGFTRLPFNKSHYVNHKPYNVPLDQRYSFINGVHKLWVYSNDKPFRSDSPTKPRSEISINGYVYSSGVWQFEGYMYVPRGTSGVSLMQLFGGNPQATTLMDNDNVKVYINGDLKFEGRGRGGNEHYFKFGVYAQDQDSSCMESRWRDVKIFKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GTQ6</accession>
    <name>A0AAD5GTQ6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Annexin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_024324</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="domain">
        <text evidence="7">A pair of annexin repeats may form one binding site for calcium and phospholipid.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the annexin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005580">
        <property type="protein sequence ID" value="KAI7752934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GTQ6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001786">
        <property type="term" value="F:phosphatidylserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009409">
        <property type="term" value="P:response to cold"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009651">
        <property type="term" value="P:response to salt stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009414">
        <property type="term" value="P:response to water deprivation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000001">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000006">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000008">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000009">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.10">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001464">
        <property type="entry name" value="Annexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018502">
        <property type="entry name" value="Annexin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018252">
        <property type="entry name" value="Annexin_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037104">
        <property type="entry name" value="Annexin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009118">
        <property type="entry name" value="AnnexinD_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10502">
        <property type="entry name" value="ANNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10502:SF222">
        <property type="entry name" value="ANNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00191">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00196">
        <property type="entry name" value="ANNEXIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01814">
        <property type="entry name" value="ANNEXINPLANT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00335">
        <property type="entry name" value="ANX"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47874">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00223">
        <property type="entry name" value="ANNEXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51897">
        <property type="entry name" value="ANNEXIN_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0041">Annexin</keyword>
    <keyword evidence="3 6 7" id="KW-0106">Calcium</keyword>
    <keyword evidence="5 7" id="KW-0111">Calcium/phospholipid-binding</keyword>
    <keyword evidence="1 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0677">Repeat</keyword>
    <feature type="binding site" evidence="6">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="254"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="296"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="298"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="299"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR609118-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7752934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="316" mass="36373" checksum="E79440175C165F4F" modified="2024-05-29" version="1">MSTIIVPTDVPPVSDDVEQLRKAFEGWGTNEGLIIEILSHRNAKQRKLIRQTYAQTYNEDLLKALDKELTRDFERIVLLWTYDPPERDAFLANEIVKNGAKSHQVIVEIACTRSSHDLLLAKKEYHARYQKSLEEDVANYTTGDIRKLLWPLVTSYRYEGEEVDMTLAKSEAKLIHDKITENCYDYDDFIRILTTRSKAQINATLNHYKDEFGQEVNKNLKADPNDEFLKILRATITSLTNPAKYFERVLRLAINKQGTDEDALTRVVTTRAEFDMKAIKEEYKKRNSLSLNEAIAKDTRGDYEDMLLALAGSDDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GW41</accession>
    <name>A0AAD5GW41_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transducin/WD40 repeat-like superfamily protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_007104</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010001297">
        <property type="protein sequence ID" value="KAI7755304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GW41"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052254">
        <property type="entry name" value="CUL4-DDB1_E3_ligase_receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44472:SF1">
        <property type="entry name" value="DDB1 AND CUL4 ASSOCIATED FACTOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44472">
        <property type="entry name" value="DDB1- AND CUL4-ASSOCIATED FACTOR 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="438"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="18"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="335"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="27"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="345"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7755304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="526" mass="59088" checksum="DD39FDC28C4DC0C3" modified="2024-05-29" version="1">MPQDLPGFYYDAEKNRYFPLKSPIPGSSSRNTSSASTTAQPLKPTKKQGKDDGQEGKVVKMVHVRELCGNSVICNKKKAGFREQYENMRVSKPTIWKYEGTGRAVDAALQHVYVNIQASEGVVESELLLTGGLNRTFSSYEAGCIGEHADYGTHHVPDVVQPLNIENQTSLKPPRLWHANGASMLIPSNLSCIKIPRNHYDPQPADVDSSVSRAILTTLGSETSGGSVYSLNLSEPLEYVAGNALFGQRFDLISSISKSTIWTADCDSNGNRTVIGTDSGAALVHMESRRKSWIFRSKSDVLSLQLDCSGNIILCGLRNGAILTVDTRQKPQDLRDKHLPKHQIQFPSIKTSQSSSGRNKKRENKWFEVRGNVHHTRSVFMPSSVSCLVALNLYDQYFLASSMDGTIRLYDHRLTQRGAVQMYEGNFNSHTRIQMGVDPSERFIMSGGEDFYLRVWRLKSGEMLYEDKFMNSVPSVVCWPRGMERFFYTFKLTGLPVIWCDTPDFWMVYVFLWTSKGHFVNAGDHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GX95</accession>
    <name>A0AAD5GX95_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">JmjC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_022285</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the JARID1 histone demethylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000411">
        <property type="protein sequence ID" value="KAI7756399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GX95"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000118">
        <property type="term" value="C:histone deacetylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032454">
        <property type="term" value="F:histone H3K9 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045109">
        <property type="entry name" value="LSDs-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12549:SF38">
        <property type="entry name" value="JMJC DOMAIN-CONTAINING HISTONE DEMETHYLASE 2, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12549">
        <property type="entry name" value="JMJC DOMAIN-CONTAINING HISTONE DEMETHYLATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02373">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="JmjC" evidence="6">
        <location>
            <begin position="1"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="50"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7756399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="155" mass="17792" checksum="08E88D355AF2955D" modified="2024-05-29" version="1" fragment="single">MAENLSFDKNINGGRDEMVDSLNPLTSDTTESNKNELDSNLSCPEGNKAEDEDVPQSDNYDSDIEYGGALWDIFRRQDVPKLTEYIKKHQKEFRGINNAPVGSVVHPIHDQTFYFDEKHKKQLKKEFGVEPWTFEQYLGEAVFIPAGCPHQVRNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GZ51</accession>
    <name>A0AAD5GZ51_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Nicotinamide-nucleotide adenylyltransferase</fullName>
            <ecNumber evidence="15">2.7.7.1</ecNumber>
            <ecNumber evidence="15">2.7.7.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">M8C21_013710</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">AA19_3_7</strain>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the formation of NAD(+) from nicotinamide mononucleotide (NMN) and ATP. Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 15">
            <text>beta-nicotinamide D-ribonucleotide + ATP + H(+) = diphosphate + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:21360"/>
            <dbReference type="ChEBI" id="CHEBI:14649"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="EC" id="2.7.7.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 15">
            <text>nicotinate beta-D-ribonucleotide + ATP + H(+) = deamido-NAD(+) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22860"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57502"/>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
            <dbReference type="EC" id="2.7.7.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 15">Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from nicotinamide D-ribonucleotide: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; NAD(+) biosynthesis; deamido-NAD(+) from nicotinate D-ribonucleotide: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 15">Belongs to the eukaryotic NMN adenylyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.1" evidence="15"/>
    <dbReference type="EC" id="2.7.7.18" evidence="15"/>
    <dbReference type="EMBL" id="JAMZMK010000005">
        <property type="protein sequence ID" value="KAI7758349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GZ51"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000309">
        <property type="term" value="F:nicotinamide-nucleotide adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004515">
        <property type="term" value="F:nicotinate-nucleotide adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09286">
        <property type="entry name" value="NMNAT_Eukarya"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000200">
        <property type="entry name" value="Nicotinamide-nucleotide adenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051182">
        <property type="entry name" value="Euk_NMN_adenylyltrnsfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005248">
        <property type="entry name" value="NadD/NMNAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045094">
        <property type="entry name" value="NMNAT_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00482">
        <property type="entry name" value="nicotinate (nicotinamide) nucleotide adenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12039">
        <property type="entry name" value="NICOTINAMIDE MONONUCLEOTIDE ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12039:SF0">
        <property type="entry name" value="NICOTINAMIDE-NUCLEOTIDE ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 15" id="KW-0520">NAD</keyword>
    <keyword evidence="9 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 15" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="5 15" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 15" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cytidyltransferase-like" evidence="16">
        <location>
            <begin position="38"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00054019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU362021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAI7758349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="250" mass="27886" checksum="F7DF368099582428" modified="2024-05-29" version="1">MEVDAALPLDKLSLDSMNEDGSSYFNTKGKTHVVLVSGGSFNPPTFMHLRLFELARDALNSKGFHVVGGYMSPVNDAYKKKGLLPAEHRISMCQLACKSSEFVMVDDWEAKQSSFQRSLTVLSRIRSFFCDNGLIPNASLKVMLVCGSDLLESFAIPGVWIPEQVRGIIRDYGVVCIRREGQDIDKIISSDEILTEYKSNIEVVNEIVPNRISSTLVRDCISRGLSIKYLTSDEVIDYIKQNQLYTNAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GZX7</accession>
    <name>A0AAD5GZX7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_019755</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000101">
        <property type="protein sequence ID" value="KAI7757558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GZX7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.110.10">
        <property type="entry name" value="Plant lipid-transfer and hydrophobic proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036312">
        <property type="entry name" value="Bifun_inhib/LTP/seed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016140">
        <property type="entry name" value="Bifunc_inhib/LTP/seed_store"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033872">
        <property type="entry name" value="nsLTP2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33214">
        <property type="entry name" value="BIFUNCTIONAL INHIBITOR/LIPID-TRANSFER PROTEIN/SEED STORAGE 2S ALBUMIN SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33214:SF44">
        <property type="entry name" value="NON-SPECIFIC LIPID TRANSFER PROTEIN GPI-ANCHORED 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00234">
        <property type="entry name" value="Tryp_alpha_amyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47699">
        <property type="entry name" value="Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical" evidence="3">
        <location>
            <begin position="18"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7757558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="81" mass="8659" checksum="ABF9D8C15CE01419" modified="2024-05-29" version="1">MGVVVHQADAQVCNLFTLSPCLGPALFFIPPSGTCCRRLRDQVPCLCDYTQGTNYGRFIGSGARRVAQACGVVSSLAFFQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BK52</accession>
    <name>A0AAD5BK52_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_030440</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012187">
        <property type="protein sequence ID" value="KAI7724561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BK52"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021138">
        <property type="entry name" value="Ribosomal_eL20_eukaryotes"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10052">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L18A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160374">
        <property type="entry name" value="RplX-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7724561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="130" mass="15314" checksum="4898D9DF44391AE7" modified="2024-05-29" version="1" fragment="single">MGSLRLRSKVQASYDFTKVLRIIWLWNTYMKKNPTTIKNYGIWLRYQSRTGYHNMYKEYKESLRNAFQSFGQLIEVLYDIDLDPFFTFRGEMDSITIGEIEGTHDELLNEAQLAAVAEEKEAHSFEIDPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BL36</accession>
    <name>A0AAD5BL36_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_018362</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010012073">
        <property type="protein sequence ID" value="KAI7725069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BL36"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000228">
        <property type="entry name" value="Serine/threonine-protein kinase BIK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050823">
        <property type="entry name" value="Plant_Ser_Thr_Prot_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45621">
        <property type="entry name" value="OS01G0588500 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="153"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="9"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="25"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7725069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="470" mass="52137" checksum="D87AF2DCB2FB3B74" modified="2024-05-29" version="1" fragment="single">KGLDENGYDQKNSSLSSSSDQQSRQVDDHGENKGPCRQFSLAEIKAATNDFDDAFVVGKGGFGKSVQSATNRKYIGKSMFIEKAWSMFLTKGPRRMSRNIKKLGQSFGNKKKSFMVAEGGGTKQAVLPRGWQTVIPKLKMFTFSELKSATKNFGQEAFLGEGGYGKVFKGWLDGATLTPQKAGDGLAVAIKKSSPNRAQGLNEWQAEVKFLGTFSHPNVVKLFGYCWENNNFLLVYEYMQKGSLDMHLFTEGAELPWDTRVKIAIGVAQGLAFLHSTQNNVICRDVKSSNILLDKASKNVDGLDSMLYFNLYQTKTYRIFQDFNAKLSDFGLAKLGPVNGESYVSTGIAGTYGRLYFKSDVYAFGVVMLEIITGLRASDYTYNGIKQNFVEWAKPSLTNIKKLQRIIDPRLDQYYPTKGVIKAAELIISCLESEPKNRPSMKEVVSSLQCINAIRVKPSQSKSKDNSTSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BQY0</accession>
    <name>A0AAD5BQY0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">60S ribosomal protein L41</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_014902</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="4">Component of the large ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the eukaryotic ribosomal protein eS32 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011496">
        <property type="protein sequence ID" value="KAI7726906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BQY0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007836">
        <property type="entry name" value="Ribosomal_eS32"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05162">
        <property type="entry name" value="Ribosomal_L41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00043969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU368055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7726906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="207" mass="23817" checksum="9260DA49422E94BC" modified="2024-05-29" version="1" fragment="single">EQAKRIVSVFDREVRYAVVTGANKGIGFETVGQLAASGVTVLLPICSFFRIIESVMVKDYAACLELCSGGSEVIENNSGVVRMQWKKKTMRRLKRKRRKMRQRPSNDNEHVICAFIARVNSGNTNLGTMLLCRLRFIWEVMEMIDLLTRRKNPKFADHYFESNYSDNHNDDHNISRADFTDWEKDFALDFIYSCSSINGLNDEITDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BQY9</accession>
    <name>A0AAD5BQY9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SPRY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_015846</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011518">
        <property type="protein sequence ID" value="KAI7726848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BQY9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000380">
        <property type="term" value="P:alternative mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12884">
        <property type="entry name" value="SPRY_hnRNP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035778">
        <property type="entry name" value="SPRY_hnRNP_U"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12381:SF56">
        <property type="entry name" value="B30.2_SPRY DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12381">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN U FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13671">
        <property type="entry name" value="AAA_33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SPRY" evidence="4">
        <location>
            <begin position="42"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="538"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="560"/>
            <end position="575"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7726848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="575" mass="64081" checksum="AD3F391CC69BDDBC" modified="2024-05-29" version="1">MPSSVKRDHFDIEGNGLQGSALYEHGFAYCWSGARADVGITGGKYCFSCKIVSGQPVDMDDTPIDQRHLCRVGISRGDDKVGNLGETEHSFGFGGTGKFSNSAKFSTYGERFDVGDTILCAVDLESEPMGSIGFAKNGKWLGTASCLVNGGEAVFPHVLLKNVVVQLQFSYEDGLVPLDGYRPWSCAFEDGKGLMGPSFSDACNCELIMMVGLPASGKTTWAERWVKDHPEKRYILLGTNLALDQMKVPGLLRKQNYGERFDRLMDRATAIFNTLLARASRRPCNLIIDQTNVYKSARKRKLQPFANFQKIAVVAFSRPEDLKARSEKRFRETGKEVPAEAVNEMIANFVLPGSKDVPGSDEYFDQVVYIELDMTESKKCLDEMKAKLQSGISISPYSSSSPVRYSQDNVLEPHRNLTYHHPLDSPTHLCHPTSGHSNTSNLQYQTISSGRHLQDRFLSRDDLNHRRSYSGYEHRAVAVSNFYQRNSSNHTPRNETMDLSPQYTVNTNYDAAPPPVERNTASSLSLYMHTPTVSPVTSIPNGSTYGAHDPMKSHGHNISHAPNYNQYQSRSSHYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BR38</accession>
    <name>A0AAD5BR38_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">M8C21_008358</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="25">AA19_3_7</strain>
            <tissue evidence="25">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RLP family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="25">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="EMBL" id="JAMZMK010011332">
        <property type="protein sequence ID" value="KAI7727724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BR38"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000275">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000015">
        <property type="entry name" value="Somatic embryogenesis receptor kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000016">
        <property type="entry name" value="Somatic embryogenesis receptor-like kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47988">
        <property type="entry name" value="SOMATIC EMBRYOGENESIS RECEPTOR KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="15 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-0675">Receptor</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9 23" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 22" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="23">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042119809" description="non-specific serine/threonine protein kinase" evidence="23">
        <location>
            <begin position="24"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="219"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="24">
        <location>
            <begin position="283"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="567"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="311"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="KAI7727724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="604" mass="67070" checksum="D8272396E53B7283" modified="2024-05-29" version="1">MRVQEKIYAFCFMFLWLWVCSNGLLSPKGVNFEVQALMSVKASLLDPHGVLDNWDADAVDPCSWTMVTCSFESSVIGLGTPSQNLSGTLSPSIGNLTNLQIILFQNNNITGPIPKEIGKLKMLQTLDLSDNKFTNEIPSSLGHLTALQYIRLNNNSLSGPIPESVANMTQLAFVIIGNPLICPTGPKQQCNGMTLMPISMPLNTTQSASTRSRKKPYKLALVIGSSLGCIFLIIFVLVLLWWRQRRNQEPFFDVKDKHHDQEVSLGNLRKFQFRELQIATHNFSHKNLLGKGGFGHVYKGTLYDGTLVAVKRLKDGGAAGGERQFQTEVEMISLAVHRNLLRLYGFCMTPAEKLLVYPYMSNGSVASRLKAKPVLDWGIRKKIALGAARGLLYLHEQCDPKIIHRDVKAANILLDDSCEAVVGDFGLAKLLDHQDSHVTTAVRGTVGHIAPEYLFTGQSSDKTDVFGFGILLLELITGQRALEFGKAANQKGVMLDWVKKIHQEKKLDVLVDKDLKNNYDMMELEELVKVSLLCTQYLPGHRPKMSEVVRMLEGDGLAERWETSQGIESTGKFRTPELSSSERYSDLTDDSSLLGQAIELSGPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BSC7</accession>
    <name>A0AAD5BSC7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PsbP C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_028976</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011353">
        <property type="protein sequence ID" value="KAI7727568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BSC7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019898">
        <property type="term" value="C:extrinsic component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009654">
        <property type="term" value="C:photosystem II oxygen evolving complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015979">
        <property type="term" value="P:photosynthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1000.10">
        <property type="entry name" value="Mog1/PsbP, alpha/beta/alpha sandwich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016123">
        <property type="entry name" value="Mog1/PsbP_a/b/a-sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002683">
        <property type="entry name" value="PsbP_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040946">
        <property type="entry name" value="PSII_PsbP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31407">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31407:SF4">
        <property type="entry name" value="PSBP-LIKE PROTEIN 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01789">
        <property type="entry name" value="PsbP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55724">
        <property type="entry name" value="Mog1p/PsbP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PsbP C-terminal" evidence="1">
        <location>
            <begin position="77"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7727568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="229" mass="25555" checksum="86A8A1D03483033D" modified="2024-05-29" version="1">MLSMQQLPCTQLGLCNNGRFKKGVSFLVRAEQVSTAPSTTQQDKVGRRQMLATGVTTAPWLLLSHQKSTVFAAENVKGFLPVTDKKDGYSFLYPFGWQEVVVEGQDKVFKDVIEPLENVSVNVFPTNKEDIRDLGSPQEVAEALIRKVLSAPSQKTKLLTAAEHEVDGKAYYTFEFVAQAPNFTRHALSTIAIGNGKFYTLTTGANERRWGKMKDKLNMVIDSFKIFKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BSE6</accession>
    <name>A0AAD5BSE6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_027581</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011403">
        <property type="protein sequence ID" value="KAI7727309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BSE6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="194"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="431"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="528"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="610"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="676"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="750"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="876"/>
            <end position="1041"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1056"/>
            <end position="1079"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="448"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="528"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="545"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="610"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="620"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="683"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="764"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="786"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="916"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="927"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="959"/>
            <end position="968"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="986"/>
            <end position="1001"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1016"/>
            <end position="1027"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7727309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="1079" mass="119995" checksum="DBBF4C70D5CC76EC" modified="2024-05-29" version="1">MNNNNNNNMAPPAAGKNGYETTPVFITTSLDTRLLTMLSPSDTVSHFKNKIMLEHIQCFPAIGNIKIHSLKVKRRGVFYHLSDSMLVNTAFGNTKGSWFVSVDASTLEQDIDKDKAGDRLALPWVTHNGSLDRHDYHGSLSIQTKTVPLENQIVSNVDGGSCKDVAKSFKENGSSDDKQLAVYKEINSRKRVRDVSNDGLPNDPSGCGLSVKKKRKTQRVKNNVEVAEETMVLMYDNDKVKDSENTFERKSKDKDLIGENTRINDHEGAGRETVADVAMVAEKNIQHGMSEKERDNELVQKAVIENEIPSLSVRTSECERDAAGLTVLGPLKGGFRRSIKHKEAEEEDRELRLKQGPEVNLPERIKKVRKSAAKNKKETPVMHADDKLVGDTSSTILAPILDEKVIGEKNEALDTTLVENSKKNDEMIVKEMEVSEPPKSSRNQVRVENMEEKSKSKLKDSVGSRKRKKDVEKTALKNDEAVIKQSDDVAKSIDGLHVDCVRNDEILMNNDVELSGIEKPLSEVRVENIDDKSEKQNEKIDGGKKSTRKKKTKKSASRNESNDVATSMDALHVDCVRNDDILMNGDVELLGTDKNLSEVKVENIDGELEKQNEKMDGVKKSTRKNKTKKIASRNEDESMTKQHVNGGIYDNVKKGDSRLPDGDREDAQHVYEGLVGKAKKNNKSSLTSPQDRLSELQSTEVELELKKSQGIDHRHVLEDTSKNVTVSEVSKVNNEVDIANDGSHEINFMDHFAPEQPDKSASIDNRKEDTKKPKKKTKDDTRNDGDIQSQKISSKNEGLVRKAKKSNKSSLTVLQDRLSKLQSTEAELESNKSQSVDHHHHLVDASKIVTGSEVSKVNNEVGITNEESSEINFMDHFAPGHQSNKLASIENEKEMKKPKKKTKDDSNIPNIDQLKTPRTTRNIDAESSSSSKSFGRSFRHRKSNKQQSAVVPVKKTLPAVNSSLNTPGNIFGDDESDRSSADDNAAVNSDCSTRTPSRVLTSSSGESDSSIDSRRNRSLVIKRKLGGKNRTSENKSKSVSMVDLLRSSSRFKKARFTASQVDDTESQPVDFVPDSQPLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BT97</accession>
    <name>A0AAD5BT97_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_018653</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MAP65/ASE1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011080">
        <property type="protein sequence ID" value="KAI7729080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BT97"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000911">
        <property type="term" value="P:cytokinesis by cell plate formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06008">
        <property type="entry name" value="NF-X1-zinc-finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007145">
        <property type="entry name" value="MAP65_Ase1_PRC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19321:SF41">
        <property type="entry name" value="FASCETTO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19321">
        <property type="entry name" value="PROTEIN REGULATOR OF CYTOKINESIS 1 PRC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03999">
        <property type="entry name" value="MAP65_ASE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7729080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="248" mass="28594" checksum="EE289FC8AB1B4D95" modified="2024-05-29" version="1">MWDIACLVEEAQSKEAPVQLLADIEEVPCYQDVVCERKCQRVRDCGRHACKRKCCDCPPCSEICDEKLRCKNLKCPSPCHSKLNMHNKSLLSTVGDHALQELAAQLTELWNLMDTSEEERSLFNHVTCNISVSVYEVIVPGSHALNLIEQAEVEVERLDKLKASKMKDIDLKKQEELEEIFAQVHIQNDKKATREKILALIVNCYLTWMHMLSKQKKKPSVEKIYYIKLKSGCQLVKNRRRELPRGLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BTS4</accession>
    <name>A0AAD5BTS4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">M8C21_008985</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">AA19_3_7</strain>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010983">
        <property type="protein sequence ID" value="KAI7729528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BTS4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033612">
        <property type="term" value="F:receptor serine/threonine kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000388">
        <property type="entry name" value="Leucine-rich repeat receptor-like tyrosine-protein kinase PXC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000454">
        <property type="entry name" value="Leucine-rich repeat receptor-like tyrosine-protein kinase PXC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000095">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050647">
        <property type="entry name" value="Plant_LRR-RLKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48056:SF40">
        <property type="entry name" value="LEUCINE-RICH REPEAT RECEPTOR-LIKE TYROSINE-PROTEIN KINASE PXC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48056">
        <property type="entry name" value="LRR RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 14" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041937552" description="Protein kinase domain-containing protein" evidence="14">
        <location>
            <begin position="25"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="547"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="621"/>
            <end position="900"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAI7729528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="903" mass="99537" checksum="EDF38A693CCB6DC7" modified="2024-05-29" version="1">MRNLCLFFILILMFLSWCQETVNSEVIIMNSDQEHVDLYTLLAIGKELKLLEWDEQNGLKNTSNYCLWPHITCSSTQSVERLVLSHLTLQGNLTLLSELKSLKWLDLSYNSFHGSIPKCFGNLYDLEFLDLSYNKFDGSVPVELGQLKKLKSLNLSNNLLIGSVPNELQGLVKLQALQLYSNHLNGSIPTWVGNLTSLRVFTAYENELSGKIPESLGAFSELELLNLHSNYLEGTIPMSVFANGKLQSLVLTQNKLSGELPDSVGKCKDLSSIRIGDNDLTGNIPKEIGNVSSLTYFEADNNNFSGEIVQEFGQCYNLTLLNLASNGFSGIIPLRFGDMMNLQELIVSGNNLSGEIPASVLGNKNLNKIDLSNNRFNGSIPESVCNSSRLQYLLLGQNSLIGEIPSAIGNCVKLLELQLGGNRLTGTIPPEIGRIKNLQIALNLSFNHLHGPLPLDLGKLDKLVSLDLSNNQLTGNIPSALKGMLSLIDVNFANNHFTGPIPTFAPFQKSPNSSFEKNTGLCGYPLNSFCGNVDGPESFHHKVSYRIVLAVIGSGLLVFLSVTIVVVLFMMREKQEKVAKTVGKEDEEIEDNNKPLVIIGNVFVENLKQAIDFDAVVKATLKDSNKISSGTFSTIYKAEMPSGLTLSVKRLKSVDKTILHQQSKMIRELERLSNLCHDNLIRPIGFAIYDDVALLLHQFLPNGSLARFLHESSKEETYQPDWPVRLSIAVGVAEGLAFLHHLAIIHLDISSGNIFLDSNFRPLVGEVEISKLLDPSRGTASISAVAGSFGYIPPEYAYTMQVTAPGNVYSFGVVLLEILTTRVPVDEEFGEGLDLVKWVQGAPTRGETPEQILDAKLSTVSFTWRKEMLSALKVALLCTDTTPAKRPKMKKVVEMLQEITESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BTW0</accession>
    <name>A0AAD5BTW0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NmrA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_012711</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010980">
        <property type="protein sequence ID" value="KAI7729542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BTW0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008030">
        <property type="entry name" value="NmrA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050608">
        <property type="entry name" value="NmrA-type/Isoflavone_red_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43349:SF16">
        <property type="entry name" value="LEUCANTHOCYANIDIN REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43349">
        <property type="entry name" value="PINORESINOL REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05368">
        <property type="entry name" value="NmrA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NmrA-like" evidence="1">
        <location>
            <begin position="10"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="NmrA-like" evidence="1">
        <location>
            <begin position="97"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7729542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="227" mass="25964" checksum="B89E0A9262255FFB" modified="2024-05-29" version="1">MAPSRAYMGHGDIEEQEGMEKIMKEHKIDIVISIVGGEAILHQLSLVNAIKSVGTIKRFLPSEFGHDVDRTDPVEPGLSMYNEKRRVRRLVEESAYFVAGSDIGKLTMKTINDTRTINKIVHFRPKANYLNINELACLWEKKIKRTLPRRIIYESDLLKAASENIIPQSIVASFTHDIFFRGCQMFSVSGTKDVDIGDLYPDECFRTMDECFDEFLMMLKEKPVDRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BW68</accession>
    <name>A0AAD5BW68_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Pyruvate, phosphate dikinase regulatory protein, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_017722</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010735">
        <property type="protein sequence ID" value="KAI7730545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BW68"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00921">
        <property type="entry name" value="PDRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005177">
        <property type="entry name" value="Kinase-pyrophosphorylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026565">
        <property type="entry name" value="PPDK_reg"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003742">
        <property type="entry name" value="PRK05339.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31756:SF9">
        <property type="entry name" value="BIFUNCTIONAL KINASE-PYROPHOSPHORYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31756">
        <property type="entry name" value="PYRUVATE, PHOSPHATE DIKINASE REGULATORY PROTEIN 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03618">
        <property type="entry name" value="Kinase-PPPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="14"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="76"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7730545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="388" mass="42584" checksum="7D1DAFE727E9DF09" modified="2024-05-29" version="1">MTPTISIEPPPTLNNPSSNHSPTTTCTKLRTSSSQLNRWSRARAIKSGRKLDRPVQPKPDPNTKTAGQESPPLEQPDPSVTSPSLSTSSSEDDTLVKGKEIYMVSDGSGRTAEHSVTAALGQFEHYLIDGGCPVYTRLFSGINDLDRLLEIVIQAAKEDAILIYTLADENLADSARRACKSWGVQSTDVLRPITDAIASYLGISPSGLPRGAPGRRFPLTDEYFRRIDAIEFTIKQDDGAFLQNLHKADIVVAGVSRSGKSPLSIYLAQKGFKVANVPIVKGVDLPKTLFEVDQKKVFALTLNPVILQTIRLERAKGLGYHDDEGSDYAEMVHVREELDYANKIFAKNPTWPVIEVTGKAIEEIAAIILRVYQDRMHGSSVPLISKLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BZW2</accession>
    <name>A0AAD5BZW2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_001369</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010240">
        <property type="protein sequence ID" value="KAI7732410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BZW2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7732410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="76" mass="8546" checksum="BFFB0D6BD13AE8EC" modified="2024-05-29" version="1" fragment="single">VQSTRRDQNRNGIRGNQATTTPHQHYARQITYCFHCIHYEGELLAIAGGHKDIKYVAHARPSMERNILEAASAIAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-10-08" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C1V0</accession>
    <name>A0AAD5C1V0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">Pectinesterase</fullName>
            <ecNumber evidence="4 10">3.1.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_026160</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 10">
            <text>[(1-&gt;4)-alpha-D-galacturonosyl methyl ester](n) + n H2O = [(1-&gt;4)-alpha-D-galacturonosyl](n) + n methanol + n H(+)</text>
            <dbReference type="Rhea" id="RHEA:22380"/>
            <dbReference type="Rhea" id="RHEA-COMP:14570"/>
            <dbReference type="Rhea" id="RHEA-COMP:14573"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17790"/>
            <dbReference type="ChEBI" id="CHEBI:140522"/>
            <dbReference type="ChEBI" id="CHEBI:140523"/>
            <dbReference type="EC" id="3.1.1.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the pectinesterase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the PMEI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.11" evidence="4 10"/>
    <dbReference type="EMBL" id="JAMZMK010009948">
        <property type="protein sequence ID" value="KAI7733537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004857">
        <property type="term" value="F:enzyme inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030599">
        <property type="term" value="F:pectinesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042545">
        <property type="term" value="P:cell wall modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045490">
        <property type="term" value="P:pectin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd15798">
        <property type="entry name" value="PMEI-like_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.40">
        <property type="entry name" value="Invertase/pectin methylesterase inhibitor family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035513">
        <property type="entry name" value="Invertase/methylesterase_inhib"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033131">
        <property type="entry name" value="Pectinesterase_Asp_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000070">
        <property type="entry name" value="Pectinesterase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006501">
        <property type="entry name" value="Pectinesterase_inhib_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01614">
        <property type="entry name" value="PME_inhib"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31707">
        <property type="entry name" value="PECTINESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01095">
        <property type="entry name" value="Pectinesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04043">
        <property type="entry name" value="PMEI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00856">
        <property type="entry name" value="PMEI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101148">
        <property type="entry name" value="Plant invertase/pectin methylesterase inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00503">
        <property type="entry name" value="PECTINESTERASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0063">Aspartyl esterase</keyword>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="5 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041778425" description="Pectinesterase" evidence="10">
        <location>
            <begin position="27"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="domain" description="Pectinesterase inhibitor" evidence="11">
        <location>
            <begin position="30"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7733537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="516" mass="57565" checksum="99F42659CE0C633D" modified="2024-05-29" version="1">MTSFNLFPSILLSVIMLLSMSTLSMQLNEETDGGINRWCQTTPHPNQCNYFLSRGPHRAPRHRSDFRLMALQAAIDNANEAQGCTKKLGKMCRTSATKAVWNDCYRLLNNTVFQLNQTITGLKNNQTSDFDTQTWLSAALTNLQTCITGCNEMNLTDFAKPIATSNLTEMISNSLAINHQFLKQKPAPNQTNDDFPTWVSRHDRRLLATVSLQSLANVTVSQVSGNRFMTIQSALDYAGSIDRQNKRFIIYIKAGVYKENIDIVNGLNNIMFVGDGIRYTIITGNRSAAGGSTTYGSATVGKILILWCRGLRIHRXXXXSASDLSVFYACSFEGYQDTLFAVSQRQFYKLCYISGTVDFIFGNAAVVFQNCVIQVRKPLTGSSNTITAQGRGDPFQNSGISIHNCRVVPAPDLIPVLGSVQTYLGRPWQQYSRTVFMKTYLDGFINPKGWLPWGNTDFAFTTLYYGEYGCFGKGSSVENRVNWTGYGTHTGVGDTHPISELCYEKSYDFIRGKLVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-02-05" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C2Q2</accession>
    <name>A0AAD5C2Q2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Calcineurin B-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_004252</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Acts as a calcium sensor. CBL proteins interact with CIPK serine-threonine protein kinases. Binding of a CBL protein to the regulatory NAF domain of a CIPK protein lead to the activation of the kinase in a calcium-dependent manner.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer. Interacts with CIPK.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the calcineurin regulatory subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010102">
        <property type="protein sequence ID" value="KAI7732966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019900">
        <property type="term" value="F:kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000073">
        <property type="entry name" value="calcineurin B-like protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045198">
        <property type="entry name" value="CNBL1-10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23056">
        <property type="entry name" value="CALCINEURIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23056:SF144">
        <property type="entry name" value="CALCINEURIN B-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00036">
        <property type="entry name" value="EF-hand_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00450">
        <property type="entry name" value="RECOVERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="68"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="150"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU369080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7732966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="198" mass="22718" checksum="006E27429C81578B" modified="2024-05-29" version="1">MGCFCSTSKDFKRLPSFDIAELAAETPFTANEVEALYELFGKLSSSMVDDGLIGKEEFHLALFRNHDKQNLFADRIFDLFDVNRSGHIDFSEFVRSLGVFHPKAPKADKVLYAFKLYDLRRTGFIEREEQLKEMVLALLNESDLELSDDIIESMVDKTFAEADSKGDGRIDQEEWREFVDKNPSLLKXXXXYTDASFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C3N6</accession>
    <name>A0AAD5C3N6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_010469</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009645">
        <property type="protein sequence ID" value="KAI7734778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C3N6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009960">
        <property type="term" value="P:endosperm development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044278">
        <property type="entry name" value="BHLH95-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46772">
        <property type="entry name" value="BHLH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46772:SF8">
        <property type="entry name" value="TRANSCRIPTION FACTOR BHLH95"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="6">
        <location>
            <begin position="115"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="155"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7734778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="314" mass="35380" checksum="58F087F389EC094E" modified="2024-05-29" version="1">MADEVSYDDAWWATHSWFTPNENVGDQQMLEHANEQKITVDANMCNENQIMKQANDNNQVVLSHGDKKETEDGDVTEKRNGKEIIDGDNIVPKRSRKGVVGFVWKRNGIGISVRRRCSSNLSAERRRRNTETKLYGELLALLPHLTVKDPKTKILEEAKNCIESLEETLRNLEKQKLERLNGASQTLVSNTESPNLLINKGASGNLDAISEPSSISFKTYVSSHVTLNVCGEAAVISICSERIPDLFTTVCYILEEHNMEVVYTEISSDHAKTMYMIRVGIRGNVPPELVEGFPYEEMYSKAVAQIEQLVTFQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C4M3</accession>
    <name>A0AAD5C4M3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_026561</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009848">
        <property type="protein sequence ID" value="KAI7734044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C4M3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7734044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="115" mass="13547" checksum="DFB99D117F9DCDCF" modified="2024-05-29" version="1" fragment="single">SLRSNLRRFLFSRTAGMRRRMVLRPLSMEEVMAERTVTREHTGPTTGIDIRSGLKKKIRIFCNPRMMDSLNDKAARKTFCFIQVHINPYVPVYIDRIQLRFFAFLFKCVTSSNRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C4T1</accession>
    <name>A0AAD5C4T1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_008316</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009492">
        <property type="protein sequence ID" value="KAI7735376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C4T1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009705">
        <property type="term" value="C:plant-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015369">
        <property type="term" value="F:calcium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004713">
        <property type="entry name" value="CaH_exchang"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31503">
        <property type="entry name" value="VACUOLAR CALCIUM ION TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31503:SF42">
        <property type="entry name" value="VACUOLAR CATION_PROTON EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0050">Antiport</keyword>
    <keyword evidence="2" id="KW-0406">Ion transport</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="32"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7735376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="59" mass="6566" checksum="48E7B42EE4A9C417" modified="2024-05-29" version="1" fragment="single">IQMSLDFGLLETGCLAFSIFLTSFTLQDGTSHYLKGVILVLAYIVIGACYFVQRVPFDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C7E5</accession>
    <name>A0AAD5C7E5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Trichome birefringence-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_004286</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PC-esterase family. TBL subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009212">
        <property type="protein sequence ID" value="KAI7736698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C7E5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016413">
        <property type="term" value="F:O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029962">
        <property type="entry name" value="TBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026057">
        <property type="entry name" value="TBL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025846">
        <property type="entry name" value="TBL_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32285:SF30">
        <property type="entry name" value="PROTEIN TRICHOME BIREFRINGENCE-LIKE 42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32285">
        <property type="entry name" value="PROTEIN TRICHOME BIREFRINGENCE-LIKE 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13839">
        <property type="entry name" value="PC-Esterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14416">
        <property type="entry name" value="PMR5N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042227953" description="Trichome birefringence-like N-terminal domain-containing protein" evidence="7">
        <location>
            <begin position="24"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="Trichome birefringence-like N-terminal" evidence="9">
        <location>
            <begin position="32"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="Trichome birefringence-like C-terminal" evidence="8">
        <location>
            <begin position="85"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF14416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7736698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="143" mass="16489" checksum="D95BF549CB679C70" modified="2024-05-29" version="1" fragment="single">MFKTSALFCLFTILVLTLTLAHAHKTKLQSNKCNFFKGSWIMDRSYPMYNGSECPFVDPGLNCQKNGRSDSMYLKFKWKPHGCSLSRFNGERFLKRNRGKKIMFVGDSLSSNQWQSLACMLYRTVRSNQTFEIMGPLSTLSFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C950</accession>
    <name>A0AAD5C950_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">E3 ubiquitin-protein ligase RMA</fullName>
            <ecNumber evidence="11">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Protein RING membrane-anchor</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">RING-type E3 ubiquitin transferase RMA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_022686</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">E3 ubiquitin-protein ligase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 11">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Endoplasmic reticulum membrane</location>
            <topology evidence="11">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">The RING-type zinc finger domain is responsible for E3 ligase activity.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="11"/>
    <dbReference type="EMBL" id="JAMZMK010009328">
        <property type="protein sequence ID" value="KAI7736236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C950"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045103">
        <property type="entry name" value="RNF5/RNF185-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12313">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 11" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 10 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="12">
        <location>
            <begin position="26"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU369090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7736236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="215" mass="24699" checksum="426C30296528A920" modified="2024-05-29" version="1">MEFENYFSDDLNPISSPEKVLPCFDCSICLDFAKEPVVTLCGHLYCWPCIYKWLSFQSDSDNNPLCPVCKSEISHTSVVPLYGRGKTLPESEPVTKTSNTVIPPRPHACLLTTPNPTQQFAYDNSQYRSYNSTTGVYSHGVEDYNGYNSNVWMYGEMIYARVFGNSQRLYTFPNSYHLAGSNTPRLRRQEMQVHRSLNRLTNFLFCCLFLCLLLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C965</accession>
    <name>A0AAD5C965_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Senescence domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_001774</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008978">
        <property type="protein sequence ID" value="KAI7737641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C965"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009686">
        <property type="entry name" value="Senescence/spartin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045036">
        <property type="entry name" value="Spartin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21068:SF50">
        <property type="entry name" value="PROTEIN EARLY-RESPONSIVE TO DEHYDRATION 7, CHLOROPLASTIC-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21068">
        <property type="entry name" value="SPARTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06911">
        <property type="entry name" value="Senescence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Senescence" evidence="1">
        <location>
            <begin position="261"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7737641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="456" mass="49645" checksum="BFACBF1DDAF2CE16" modified="2024-05-29" version="1" fragment="single">YISHSVIYSSTCFHPFLHFHVNLHKNPRIQTLITNIIIQIKMASQNLYPKINQSNPNTSSYPTPTPSAPSLYPSLDFTDLHEDLFIDTQQSHDPHTVTSVQSSEEIIIQIPNSIIHLIDKQQSIELASGVLQIVRLRQGDAVIAVFARVNDVIQWPLAKDVSAVKLDGSHYFFTLRVISDTDSDSETDNMLNYGLTIVNGDGLGEFDRVLDEYSAFSVKEVKRNVVVVDDGGSSTVEAKANAYWTTLAPNVEDYSGSVARAIAAGSGQLIKGIMWCGDVTVDRLKWGNEVLKKKRKPGVKAEVSPQALKRMKRVKRLTKMSEGMATGVLSGVVKVSGVVTGSIVNSKPGKKFFNLLPGEIILASLDGFNKVCDAIEAAGKNVMSTTSTVTTDYVSHRHGEDAAKLNHEKMSAAGHAIGTAWAVFKIRQALNPKSAIKPTTLVKAAASQVKSSKSKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CAK4</accession>
    <name>A0AAD5CAK4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Trichome birefringence-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_027844</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PC-esterase family. TBL subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008972">
        <property type="protein sequence ID" value="KAI7737675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CAK4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016413">
        <property type="term" value="F:O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029962">
        <property type="entry name" value="TBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026057">
        <property type="entry name" value="TBL_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32285:SF264">
        <property type="entry name" value="PMR5 DOMAIN, PC-ESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32285">
        <property type="entry name" value="PROTEIN TRICHOME BIREFRINGENCE-LIKE 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13839">
        <property type="entry name" value="PC-Esterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Trichome birefringence-like C-terminal" evidence="2">
        <location>
            <begin position="57"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7737675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="202" mass="23026" checksum="E10A100197BF88C5" modified="2024-05-29" version="1" fragment="single">LQCDDILVPHSIFGGHRETKHRKSFAFGFDQWRRYMERYGCARVQLVALVDPYWQFDYIQDGSTISKDMDRLAAYNKGMSTWGKWVDLNVDPSKTKVFFQGISPTHYMGKEWGSSSRNCYGELQPIEGSSYPAGLPEAQVVLNRVLSSIKKPVTLLDITTLSQLRKDAHPSTYSDTGVDCSHWCVPGLPDTWNQLMYASFLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CBI1</accession>
    <name>A0AAD5CBI1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_014549</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008871">
        <property type="protein sequence ID" value="KAI7738140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CBI1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7738140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="95" mass="10661" checksum="24EEFAF78F279C21" modified="2024-05-29" version="1">MTSFQPKGGPLSPDELISLIFNLLLGIRRVSFTGLNFKLRSRSVIQIQSICAVGEELHRLLNVSKKNVKGLNVTFATSFVGYENEEWVSQFGEKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CCF2</accession>
    <name>A0AAD5CCF2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Transport inhibitor response 1-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_002386</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008707">
        <property type="protein sequence ID" value="KAI7738869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CCF2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009734">
        <property type="term" value="P:auxin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22159">
        <property type="entry name" value="F-box_AtTIR1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000029">
        <property type="entry name" value="Transport inhibitor response 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041567">
        <property type="entry name" value="COI1_F-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041101">
        <property type="entry name" value="Transp_inhibit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16134">
        <property type="entry name" value="F-BOX/TPR REPEAT PROTEIN POF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16134:SF36">
        <property type="entry name" value="TRANSPORT INHIBITOR RESPONSE 1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18511">
        <property type="entry name" value="F-box_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18791">
        <property type="entry name" value="Transp_inhibit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0927">Auxin signaling pathway</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="COI1 F-box" evidence="7">
        <location>
            <begin position="42"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="Transport inhibitor response 1" evidence="8">
        <location>
            <begin position="101"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF18511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF18791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7738869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="613" mass="68264" checksum="A4E3B30C5A05F402" modified="2024-05-29" version="1">MSDDTSPGCATTAAAGSGGSSGKVRAPVSSSGDGGSFTAHPDQVLENVIENVLVFLTSRRDRSTVSLVCRSWYRAEAYTRSDLFIGNCYAIAPNRVTDRFKRIRSVSLKGKPRFADFSLLPADWGAYFTRWVHAMSLAYRALEKIYLKRMSVTDDDLSVIARSFPCFKELVLVCCEGFGTSGLAVVVSECRNLRVLELIEDEVSDDETDWISCFPEGTTNLESLCFDCVEAEVNFDALEKLVARSPMLKKLKLNMFVSIGELYRLVVRAPQLTHLGTGAFNPLVEIGQQQGDNQELDYTSLFAACKSIVCLSGFREIAPEYFPTIVPLCGNLTSLNLSYANVNAEQFKPVIRHCHKLQVFWMDSICDEGLQAVADTCKDLRELRVFPVDPHEYDEGPVSEVGLLAISLGCRKLQSILYFCQQMTNAAVIAMSKNCPDLVVFRLCIIGRYRPDRVTGEPMDEGFGAIVKNCKKLTRLAVSGLLTDKAFSYIGQYGKLVRTLSVAFAGDSDAGLKSVLEGCTNLQKLEIRDSPFGDSGLQPGLHQFYNMRFVWMSSCRVTRQWCLEVARRLPKLVVEVFSRDEEEGERGDFVETLYMYRSLDGPRPDAPQFVEIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CCZ7</accession>
    <name>A0AAD5CCZ7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_007554</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008860">
        <property type="protein sequence ID" value="KAI7738171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CCZ7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd10017">
        <property type="entry name" value="B3_DNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.330.10">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003340">
        <property type="entry name" value="B3_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015300">
        <property type="entry name" value="DNA-bd_pseudobarrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46245">
        <property type="entry name" value="B3 DOMAIN-CONTAINING PROTEIN OS07G0563300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46245:SF10">
        <property type="entry name" value="B3 DOMAIN-CONTAINING TRANSCRIPTION FACTOR VAL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101936">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7738171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="445" mass="48785" checksum="0C490F4F4492EB93" modified="2024-05-29" version="1">MLLKMENSVIHITQMLQVGGVVKLVESVISHVHIVGCWRHRVSQTPNPTWPSASHFLHGPAERINDVSSKSWRSIAGSGPVPWRQAPSLFNASKGQPELQLTSCSLGRSTRDDPSGRYVTDSWRVSAQELIGDKATSGIQYDKKQNLFNDVSYQSFFHTSVTPLSTLPATFALHNQKTETANVSGIRVKRLCTPQSVGKQCSNNDGAGPSLGNLAHNTKARDETMSCHQLLSYYGSQLTDQELPQLPAGWLVALNVLSASDAGKIGRLVLPKKCAEAYLPPISQPEGCPLIIQDLKGKDWKELLPVYGQCSCKLAILLLLIRYVGLVTFSRLDPEGKLVMGFRKALSASPSDKGNRKTNARISAREESLKDGTLSEVDKTSIHAKRKKGANMCSNSKTLRFSVTLEQIQGLLRPPLTKAPTIVLFEGVEFEVIDIAFTSEPILKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CE47</accession>
    <name>A0AAD5CE47_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_029948</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008562">
        <property type="protein sequence ID" value="KAI7739838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CE47"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022228">
        <property type="entry name" value="DUF3755"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14000">
        <property type="entry name" value="FINGER CCCH DOMAIN PROTEIN, PUTATIVE (DUF3755)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14000:SF43">
        <property type="entry name" value="HOMEODOMAIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12579">
        <property type="entry name" value="DUF3755"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7739838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="257" mass="28768" checksum="DA905B92FDE387A8" modified="2024-05-29" version="1">MDTGFRYDRVISFQSGGVKSASENMGMMMGHYYPMNIAAVTRTGYLGTQSGNSCGSVQGLKHDAGLAVEWSVEEQRRLEEGLSKFASEPNIMRYIKIAATLNDKTARDVALRCRWMARKRRKQDNMVVSSSSKPSISSVPTFDVAPFSVAMKNRVQIDSNTFEALSGSIKHLLEQNNQVLGQISANISSMKLQDNLDLFSHTKNNITAILNDMKYMPGPAFPVSINEDLAQNILRPTNQRMTFEPSNGMYMKREQEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CEF1</accession>
    <name>A0AAD5CEF1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein pleiotropic regulatory locus 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_024690</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the WD repeat PRL1/PRL2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008428">
        <property type="protein sequence ID" value="KAI7740447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CEF1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000012">
        <property type="entry name" value="Putative pleiotropic regulator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045241">
        <property type="entry name" value="Prp46/PLRG1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19923:SF0">
        <property type="entry name" value="PLEIOTROPIC REGULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19923">
        <property type="entry name" value="WD40 REPEAT PROTEINPRL1/PRL2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="164"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="206"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="248"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="290"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="423"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="63"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="105"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="457"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7740447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="478" mass="53084" checksum="769ED2C2917BE959" modified="2024-05-29" version="1">MTAPTMASELPVEPQSFKKLSFKSLKRSLDLFSPTHSLLPPPDPDSKKIRLSHKLHVEYGGIQTVSNPPVKSSLQNQVAPSNSNSLVLSGSTNSEKTGAQSDLAITSHQPKNLGDGNKTGKSTAVIPARGTSERNLPTAALMERIPSRWPRPVWHAPWKNYRVISGHLGWVRSVAFSPNNEWFCTGSADRTIKIWDVGSGRLRLTLTGHIEQVRGLAVSTRHTYMFSAGDDKQVKCWDLEQNKVIRSYHGHLSGVYCLALHPTIDVLLTGGRDSVCRVWDIRSKMQIHALSGHDNTVCSVFTRPTDPQVVTGSHDSTIKFWDLRYGKTMATLTHHKKSVRALAQHPIEDSFASASADNIKKFSLPRGEFLHNMLSQQKTIINAMTVNQEGVLATGGDNGSLWFWDWKSGHSFQQSQTIVQPGSLDSEAGIYALSYDMTGSRLVTCEADKTIKMWKEDENATPETHPVNFKPPKDIRRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CEI6</accession>
    <name>A0AAD5CEI6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_011416</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008525">
        <property type="protein sequence ID" value="KAI7740078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CEI6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006566">
        <property type="entry name" value="FBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055357">
        <property type="entry name" value="LRR_At1g61320_AtMIF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31639:SF326">
        <property type="entry name" value="F-BOX DOMAIN, FBD DOMAIN, F-BOX-LIKE DOMAIN SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31639">
        <property type="entry name" value="F-BOX PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08387">
        <property type="entry name" value="FBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23622">
        <property type="entry name" value="LRR_At1g61320_AtMIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00579">
        <property type="entry name" value="FBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="8"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7740078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="345" mass="39789" checksum="E9CD97B43876F886" modified="2024-05-29" version="1">MEVERLPLDRISSLPPNIIQHILTLMPMRDAFRTSVLSQSWRDHCLNIPKLTFDDALFQGSSYKTVSIKCKLLHVVYPILLLHQGAILEFSLSLFGLESCREIDQIILHLSRNATLKEFTLRVGSGDEHKLLSAFFKLQQLTVLKLENCAFQPPVSFKGFSRLVTLSIDSVSINVEVFLRFLSNCPLLKHLTLFFARSVMPEKLPAFLVHLKHLRIPGRYSFEREIALLCAYVLVTINSSTMETILMEMPHCPGKAMPAGIDFDKYQNHPCIILHRLRVITITNFLNIKKEMDFVKFIVARSPMLKEVHIVVNKRVDIHDEVKMLKELLRYPRASVRAEIRFERP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CEZ4</accession>
    <name>A0AAD5CEZ4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Splicing factor Cactin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_010712</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CACTIN family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008628">
        <property type="protein sequence ID" value="KAI7739369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CEZ4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019134">
        <property type="entry name" value="Cactin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018816">
        <property type="entry name" value="Cactin_central"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21737">
        <property type="entry name" value="POLYGLUTAMINE BINDING PROTEIN 1/MARVEL MEMBRANE-ASSOCIATING DOMAIN CONTAINING 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21737:SF4">
        <property type="entry name" value="SPLICING FACTOR CACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10312">
        <property type="entry name" value="Cactin_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09732">
        <property type="entry name" value="CactinC_cactus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01050">
        <property type="entry name" value="CactinC_cactus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Splicing factor cactin central" evidence="6">
        <location>
            <begin position="198"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="domain" description="Splicing factor Cactin C-terminal" evidence="5">
        <location>
            <begin position="544"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="414"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="162"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="66"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="84"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="414"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00034534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF09732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF10312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7739369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="684" mass="80834" checksum="85AD79067DEF1D1C" modified="2024-05-29" version="1">MSSSRSKKDESKRRKTYDDDTDESDYSSPRRRSTKARDRSRSSKHSSRRYSDSEDDSDESDSSPKQRSRKSRERSRSSKRSRRRDSDSDSEYSKDDSDDDRRSKKKKSKKISEEEIAEYLAKKAQKKAMKVAKKLKFVWRKKIERDVTQGVPLDMFSIKAEKKRQKERMAEIEKVKKRREERAIEKAQHEEEMALLARERARAEFQDWEKKEEEFHFDQSKVRSEIRLREGRIKPIDVLSKHLDPTGEFDMEIEEPYMVFKGLTVKEMEELQEDIKMHLDLDRGTPTHIQYWEALVVVCDWELAEARKKETLDRARVRGEAPPADIVAEERGMHSSIEADVKKLLDGKSFAALEQLHSQIESEMRSGKAKVVEYWEAVLKRIHIYKAKACLKEIHNKMLRKHLEYLEQPDRSFNRDMERDKTVSPKVDQESDHDLEGNEAPEVIPRGEQQAMEEEEAGSFSPELLHDDEDEEAIDPEEDRAILERQRIQVVEEQQRKLQEAMALPPPEDNLEMRAAKAMGAMDNGDEVFGNSDEVNVDSQVYWWHDKYRPRKPKYFNRVHTGYEWNKYNQTHYDHDNPPPKIVQGYKFNIFYPDLVDKTKAPVYTIERDGDSAETCIIRFHAGPPYEDIAFRIVNKEWEYSHKKGFKCTFERGILHVPKMSMYLIIFMCVPRSERHKHNFTRHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CFJ7</accession>
    <name>A0AAD5CFJ7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein NRT1/ PTR FAMILY 6.3-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_014714</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Phosphate:H(+) symporter (TC 2.A.1.9) family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Proton-dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008362">
        <property type="protein sequence ID" value="KAI7740692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CFJ7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006857">
        <property type="term" value="P:oligopeptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000109">
        <property type="entry name" value="POT_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018456">
        <property type="entry name" value="PTR2_symporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11654">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00854">
        <property type="entry name" value="PTR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01022">
        <property type="entry name" value="PTR2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="42"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="68"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="110"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="152"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="182"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="302"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="342"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="385"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="470"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="512"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7740692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="554" mass="61228" checksum="621B52BA9C1A4313" modified="2024-05-29" version="1">MILGVEACERLATLGIAVNMVTYLTGTMHIGNANAANDVTNFMGTSFMLCLLGGFLADTFLGRYLTIAIFTAIQALGVTVLTISTAIPSLQPPKCTQTGGSCVPATDLQLAILYLALYLTALGTGGLKSSVSGFGSDQFDESNKEEKAQMTAFFNWFFFFISIGSLAAVTVLVYIQDNLGRRWGYGIVACSIGLGFVIFLSGTPRYRFKKLVGSPLTQIASVFIAAWRRRHLELPSDPSLLFNVDDVQVADDPRKLKQKLPHSKQFRFLDKAAIKKNEKSGEISKMTKWHLSTLTDVEEVKMVITMLPIWATTIMFWTIYAQMTTFSVSQATTMDRHIGKSFLIPAASLTVFFVGSILLTVPVYDRVVVPLAKRFLKNPQGLSPLQRIGVGLVLSTFAMVSAALIEIKRLRMAQSHGLVDDPSKVVPLSVFWLVPQFFFVGSGEAFTYIGQLDFFLRECPKGMKTMSTGLFLSTLSLGFFFSSLLVTIVHKITGDGHPWLADNLNKGKLYNFYWLLTVLSVINMGLYLLGAKWYVYREHRLAAGSIELEEDQVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CI16</accession>
    <name>A0AAD5CI16_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_027218</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Functions as an E3 ubiquitin ligase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="JAMZMK010008325">
        <property type="protein sequence ID" value="KAI7740901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CI16"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16664">
        <property type="entry name" value="RING-Ubox_PUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000082">
        <property type="entry name" value="RING-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000442">
        <property type="entry name" value="RING-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058678">
        <property type="entry name" value="ARM_PUB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057623">
        <property type="entry name" value="PUB12-19-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045210">
        <property type="entry name" value="RING-Ubox_PUB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003613">
        <property type="entry name" value="Ubox_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23315:SF49">
        <property type="entry name" value="RING-TYPE E3 UBIQUITIN TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23315">
        <property type="entry name" value="U BOX DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25598">
        <property type="entry name" value="ARM_PUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25368">
        <property type="entry name" value="PUB10_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04564">
        <property type="entry name" value="U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00504">
        <property type="entry name" value="Ubox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51698">
        <property type="entry name" value="U_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="U-box" evidence="9">
        <location>
            <begin position="152"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="8">
        <location>
            <begin position="284"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="8">
        <location>
            <begin position="366"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7740901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="530" mass="59229" checksum="31979B80133C5214" modified="2024-05-29" version="1">MITRFNTVYDKLSHAMDGFPYDEIGISEEVKEQLELMCMQLKRAKQRIDSQDMELTMDMRAVLSFDSDRIAECESIEKLANRLPLNTLEDLRIETVAIRKLVKERRGQNAQGTQNIIDLLDRFKRFAGIEQVNVLDEPVRRNRSLQKCTSIAIPFEFLCPITLEIMRDPVIVATGQTYERANIQQWLDSDRRSCPKTGQTLAHTALAPNVALHKLIQQWCQTNNYQISKKAPPPPPKKAHSEKVIILIQNLYSSELQVQRKAVTKIRMISRESPENRVLIAEKGGIPPLVLLLSYPDSKIQEHAVTALLNLSIDEKIKKQVSEEAPIPAIIEILQYGTTGAKENSAAALYSLSMLDENKALIGSSNGIPPLIALLAEGTIRGRKDAATALFNLTIPQSNKAKAIEAGAIQPLLSMLENKGLDMVDEALSVLLLLAEHPDGRKELGQLSFIETLVKFMREGTPKNKECAASVLLKLCENNNNNLVVALQYGVYEHVSYISHNGTKRGKKKAAAMLQLMSKSGQIPSYNNER</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CJG6</accession>
    <name>A0AAD5CJG6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Heparanase-like protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_022138</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Endoglycosidase which is a cell surface and extracellular matrix-degrading enzyme. Cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Lysosome membrane</location>
            <topology evidence="9">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 79 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007844">
        <property type="protein sequence ID" value="KAI7742982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CJG6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009505">
        <property type="term" value="C:plant-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004566">
        <property type="term" value="F:beta-glucuronidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000023">
        <property type="entry name" value="heparanase-like protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005199">
        <property type="entry name" value="Glyco_hydro_79"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14363:SF43">
        <property type="entry name" value="GLYCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14363">
        <property type="entry name" value="HEPARANASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03662">
        <property type="entry name" value="Glyco_hydro_79n"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0458">Lysosome</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042267316" description="Heparanase-like protein 3" evidence="11">
        <location>
            <begin position="27"/>
            <end position="534"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00055929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7742982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="534" mass="58715" checksum="1507AC32A6D4AC87" modified="2024-05-29" version="1">MGSLSSQMGICILAFLFIFGSVFVDSVENGSVFIDGANGIAETDSNFICATMDWWPPEKCDYGSCSWGLASLLNVDLNNTIFRNAIKAFSPLKIRLGGSLQDELVYETEDQTEPCIPFSQNTSALFGFNPGCLPLSRWDELNIFFDETGAKIIFGLNALNGRQLNADGSTVGAWDSTNAESFMRYTVKRNYKMYAWELGNELCGSGVGARVTADQYAIDTTTLKDIVEEIYDGIEPKPLIISPGGFFDANWFKEFINKTTDILDVVTHHIYNLGAGVDKDLVKKILDPSILDGLADPFKQLEKVLETSGTSAVAWVGEAGGAYNSGHHLVTDAFVFSFWYLDQLGMSAVYDTKAYCRQSLIGGNYGLLNTTTFEPNPDYYSALLWHRLMGQTVLSTNFTGSKKMRSYAHCAKESDGITLLLINLDNTSTIDVNLSDDTLLKSRAHIEMVIQDTETNGSKTREEYHLTAKDGNLHSQTMLLNGNELTVNPDGDIPPLEPLHVSSLEPISVAPYSIVFAHIPYLTLNACGSLVTDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CJM6</accession>
    <name>A0AAD5CJM6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">No apical meristem-associated C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_022906</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007992">
        <property type="protein sequence ID" value="KAI7742425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CJM6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45023">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45023:SF4">
        <property type="entry name" value="GLYCINE-RICH PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7742425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="403" mass="45830" checksum="EB565B86131A7112" modified="2024-05-29" version="1">MGLITTSMVTNIITNTLLAFDYRNCHPMNTTSKKKKRKGDTESTYKRQPWTIADDELLTKGFLHVSKGSVVGNGPKSLDFWKRVLKYYTEGESSGAQRTADNLRSHWHMIKNNVTSFNNIMIQLRAQKGSDWCDDELKTQAHKIYHEEHKSHFIREHVWNLVKDEPKWKTHIEIKEPKRTKTSPLGEYTTSSDVNTCIKLENDIDDVSISSEDNDVDVLPCHMAMEKDNVVDALPLRMSTEKYNVVDALPLHMSTEKCNGVDALPHSMGTEKDNIVHALPLRMGIEKCNGVDVLPHHMGTEKDNGVDVLPHLKGTKKAKTKGTLKGKCVKEVTAALDDMLHKKVDLMEQLNAKMAQVIALKEKKLAMSEMEILYKDTSQLDPETLAFHKERCAMIREKYGKMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CJR5</accession>
    <name>A0AAD5CJR5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_003247</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008133">
        <property type="protein sequence ID" value="KAI7741814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CJR5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7741814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="30" mass="3491" checksum="4D6A66295856AB2B" modified="2024-05-29" version="1">MEPPRISALKPKEEASAIDVRIPRKWIPDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CJV6</accession>
    <name>A0AAD5CJV6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">F-box/FBD/LRR-repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_008724</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007856">
        <property type="protein sequence ID" value="KAI7742919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006566">
        <property type="entry name" value="FBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055411">
        <property type="entry name" value="LRR_FXL15/At3g58940/PEG3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31639">
        <property type="entry name" value="F-BOX PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31639:SF315">
        <property type="entry name" value="LEUCINE-RICH REPEAT DOMAIN SUPERFAMILY, F-BOX-LIKE DOMAIN SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08387">
        <property type="entry name" value="FBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24758">
        <property type="entry name" value="LRR_At5g56370"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00579">
        <property type="entry name" value="FBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="4"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="163"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="427"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="domain" description="FBD" evidence="3">
        <location>
            <begin position="733"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="831"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="domain" description="FBD" evidence="3">
        <location>
            <begin position="1298"/>
            <end position="1370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1188"/>
            <end position="1207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1188"/>
            <end position="1202"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7742919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1370" mass="157648" checksum="A7D7AC383A63523F" modified="2024-05-29" version="1" fragment="single">ISKLPLGVIETILCFLPIHEATRTSILSREWRYHWTTIPKLVFNEDMFKGPIHEFTLSMRADGSCVEIDHIIRHLSKKTTIKILKLEFIGVYRLPLSLFSLHKLTGLYLNGCVLDHQPSSNVFRSLTTLCLDNIRTCKTELLRLVSSCPSLKRLTIMDKISKLPPGIIENILCLLPIQEAARTSILSKEWRYHWINIPQLAFIEDKFEASTDETEPSVLEQTFDRPSKRKDMAKRCKLFYAIYQVFLVHQGPICDFTLSMRVDGSCVEIDHIILHLSKKISVKMLKLDFIGRYKLPSSFFSLHQLTDLYLNDCALDPPPSFSGFGNLTTLYLQEICISDKALLRLLSSCPSLKRLTINSDGSTITGTGAFTIVDLFECLPVIEYLSIWFYIVMFFDPDRLPKKLPTTLVHLKYFQQECSKMDRISKLPIGVIETILCLLPIQEAARTSILSREWRHHWTKIPKLAFDEYTFEVSTYAAELSVLERTFVRRSHRKVLTTRCKLFYAIYQVLLMHQGPIHEFTLSMIADDSCVEIDHILLHLSKKNTIKILKLDFGGRYMLPLSLFSLHHLTDLSLSNCDLDHQPSSHVFGSLTSLHLQELQTSKKALLRLLSSCPLLERLTLMSDGGTIDDSGDSTIADLFKCVPGIEYLSFWFFVFLLFLPLPKELPTTLVHLKYLYLDGVWFRHEYAVPFLDLLIRSSPNLEKLKLVISGEDDLFDDCYVKIHSFHREDYSDFVLEHLNELEILDFSDALNELGFVKLILAKSPVLKKVRILLWDQLDKDVKLRISKIILSYPCASPVATRGGPQQTTRDGQNGEFKEEMLYKMDRISKLPLGIIETILCLLPIQEAARTSILSTEWRNRWTTIPKLVFIENKFQEWTHGPVLSAAEQYLNKPSPIHEFTLSMEVDGTCVEIDHILLHLSKKNTVKILKLDLDGGLDEGHRLPISLFSLPQLTSMYLNGCALYQERSFNEFGCLTTLYLGSVWTYAKTLLRLLSSCPSLKRLTLNCECSVIFRSSDTTIVDLFKCLPLIEYLRVVLFIVECFDPEILPKELPITLMVHLKHLRVDSFCFSHKYGLPLLVPLIRNSPNLEKLKLVMEDIDWPDEDDIGSFKLEDYSDIMFEHLNEFESIHFSDAENELDFVKLILAKSPMLKKLYEMDRMSKLPLGIIETILCLLPIQEAARTISTDRAQAQPSVLEQTSNRPSEKERMTKRSKIFNAIYQDCEFSVIFGRDTIADLFKEIFERAPNNISPHEIQGLPLLALLIRSSPNLEKLKLVLYNTNWLEDEEIGSFTLDDYSDIMLEHLKELEFQDFGDQENELDFVQLILARSPVLKRVSIFTYRLFDVDEKPEILKILCLSPRASPVVEIMVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-10-08" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CK70</accession>
    <name>A0AAD5CK70_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_003229</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007742">
        <property type="protein sequence ID" value="KAI7743348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034005">
        <property type="term" value="F:germacrene-A synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016102">
        <property type="term" value="P:diterpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046246">
        <property type="term" value="P:terpene biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00684">
        <property type="entry name" value="Terpene_cyclase_plant_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.600.10:FF:000007">
        <property type="entry name" value="Isoprene synthase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.130:FF:000001">
        <property type="entry name" value="Isoprene synthase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.130">
        <property type="entry name" value="Terpene synthase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034741">
        <property type="entry name" value="Terpene_cyclase-like_1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044814">
        <property type="entry name" value="Terpene_cyclase_plant_C1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001906">
        <property type="entry name" value="Terpene_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036965">
        <property type="entry name" value="Terpene_synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050148">
        <property type="entry name" value="Terpene_synthase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005630">
        <property type="entry name" value="Terpene_synthase_metal-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31225:SF250">
        <property type="entry name" value="(-)-BETA-CARYOPHYLLENE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31225">
        <property type="entry name" value="OS04G0344100 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01397">
        <property type="entry name" value="Terpene_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03936">
        <property type="entry name" value="Terpene_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01019">
        <property type="entry name" value="Terpene_Cyclase_Like_1_C_Termi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Terpene synthase N-terminal" evidence="3">
        <location>
            <begin position="18"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="Terpene synthase metal-binding" evidence="4">
        <location>
            <begin position="253"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7743348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="548" mass="63800" checksum="80F57C712279B2E3" modified="2024-05-29" version="1">MSFQQEVIRPLRDVQPSVWGDQFLNYNKQPQQDEEDQIVHDLKEDVRKALLTALAVPNEHTRLLKLIDAIQRLGISYYFEDEIDKALQHVYDAYGEDWNGGSPSLWFRLLRQHGFYVSCDVFNNYKDEQGAFKESLTGXXXXEELIELYEATYMRVHGEAVLDKALSFTRAHLGEIAKDPIRCNSTMSAHIQEALDQPIRIRLPRLEALRYIHFYQQQASHDESLLKLAKLGFDLLQSLHKEELSELSKWWKGIDISNNLPHVRDRLVESYFWALGGYCEPKYSFARMFFTKFILVATILDDTYDAYGTYKELEILTEAILRWSVTCLDELTGYMKVVYQVLMDMYNEREEILEKEGKIDLFNCSKEYVKTFVRGQLMEAKWANEGYIPTTEEHDYVAFKTGSGGMLITACYLGMTDLVTNETVKWAITDPPLFKASTKVGRFLNDIASHKDEQERNHFASTIESYKNQYHYTDEHVYDLMRKEVEDVWKDINLESVTRKDVPMPLIMVGVNLTRLLEILYKDGDNFTHPGEQFKNIVKSVLVHPISI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-04-02" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CLP5</accession>
    <name>A0AAD5CLP5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein EARLY FLOWERING 3-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_015072</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007583">
        <property type="protein sequence ID" value="KAI7744128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000028">
        <property type="term" value="P:regulation of photoperiodism, flowering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039319">
        <property type="entry name" value="ELF3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34281">
        <property type="entry name" value="PROTEIN EARLY FLOWERING 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34281:SF2">
        <property type="entry name" value="PROTEIN EARLY FLOWERING 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="155"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="520"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="114"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="162"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="189"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="523"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="549"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="578"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="593"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7744128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="621" mass="68398" checksum="C5C59A58CEFDBEA3" modified="2024-05-29" version="1" fragment="single">MKRGKDEDKTMGPMFPRLHVNDTEKGGPRAPPRNKMALYEQLSIPSQRFNSGVASSSNRASVNERGTFSPRQQSSSFHPIRKPDTRHSDLNNQRVQQEHKKRQEEDDFRVPIFDQQSGTNQNRNVNHQNRENGGTTPFGAALSGRLANVHNAKPHNNKFQKENSQGFARSASNQSSRQNVKAPLKETNESSSFNHRNASNSRNLRDYRADSQADNTLWGDSLLNEASKASGYGNNSVPLREIQEEALRNPDDPTNGDAVSENSMVDSICGVDISPDDVVGIIGQKHFWKARRAIVNQQRVFAVQVFELHRLIKVQRLIAESPQLLVDENGYLAKPPKVSPIKKLPFEYVMKPTVNIPKHKNDIEKLNDDMEFSSENAVGKASLSTVQNGSQTTNCRPFGGTPLPPSDQNMGSWNFNAPPGHQWLIPVMSPSEGLVYKPYPASGFMSPAYGGCGPPGSMPVIGTXXXXDLKDPILQTMVHHLLIMKGSQGLTPSILHFPLAMATSLLTVCKWLXXXXSARFEPSNPNNQGSTNIPVEKTRPTVPKVVRLNNNNNKNNNNNNNNTASKDTEVQASTASSRSDRSNDRNALRLFPTSPPPAPAPAAAPEPVRVEAAGPSRVIKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CLX3</accession>
    <name>A0AAD5CLX3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_010412</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007852">
        <property type="protein sequence ID" value="KAI7742941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CLX3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057024">
        <property type="entry name" value="Znr_FGT1_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057025">
        <property type="entry name" value="Znr_FGT1_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23547">
        <property type="entry name" value="Zn_ribbon_FGT1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23548">
        <property type="entry name" value="Zn_ribbon_FGT1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FORGETTER1 first zinc ribbon" evidence="2">
        <location>
            <begin position="90"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="FORGETTER1 second zinc ribbon" evidence="3">
        <location>
            <begin position="133"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7742941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="256" mass="28564" checksum="0CFF102D77B373B7" modified="2024-05-29" version="1">MEPPPEPLLPPQPQQPPPPYYYPLPMEPSPQPQQPPPYYYPIPMEPPPQPQQPPKLPQQPQQPQQPPQLPQPPQPQPQSQPQKQPKKQQRVRCSGCKKKSTVGPSLKEFVCSKCHLKQDVVRPRKRPRVSHPQVPCVNCKATVTVPHGLPRFPCPHCNTELQAPPPPLPEEVNEVAIEVEQEEDNGGVVGETFTDYCHKAKNLVPEAGQKPTYTGKAVLELQFLKCCCLDMGVEMSIVKENIGVLFLKEIGVAAVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-10-08" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CM04</accession>
    <name>A0AAD5CM04_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">M8C21_011831</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">AA19_3_7</strain>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007710">
        <property type="protein sequence ID" value="KAI7743625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000161">
        <property type="entry name" value="Wall-associated receptor kinase-like 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46008">
        <property type="entry name" value="LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-LIKE 1.4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46008:SF25">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5 14" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042238348" description="Protein kinase domain-containing protein" evidence="14">
        <location>
            <begin position="21"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="261"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="326"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="355"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAI7743625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="678" mass="76834" checksum="51B12A7CB0BDAB1D" modified="2024-05-29" version="1">MIRVFVFVSVSLTCLQLLHSASDKNDSVPTCPESFTCPGLATFNYPFYNVTDTRCGLIKVNCTLTGAEIEFGGRLYEIVRYLNYERYYGALLVWSRTFEQLVRNKSSACEALMYNLTSPNPSPRLYNISIAPFITLLKCAKNITFSEQRNYYSYNDCNDHNFYYNLNGTVSSDIPHQCQVVQLTGKNLSSQALDQTNIFSLLSASSFINYNLSPFCYECQKNDRQCVAENEHDIQCLDVKKVNPGREESNQKKHGRKLTTMILAIVGSLLILILPFVIFIIWRRCKSRPFSYVSSKNKSQNDEDISLSSGVSVFSYKELEDATKNFDPSRELGDGGYGAVFYGKLKDGREVAVKKLHEHNYNRVQQFRNEIEILTKLRHPNLVVLYGCTSRQSHELLLVYEYVPNRTVADHLHGDQANPNLLTWPIRMNIAIETASALVYLHASEIIHRDVKTANILLDHNFCVKVADFGLSKFISHNAPHLSTAPQGTPGYVDPQYHQRFQLTDKSDVYSFGVVLIELISSMVAVDFDRSQDEISLANLALNKIQRCAIDELIDPVLGSDANLETMNMITLVAELAFRCLQFDSDMRPTMNEVLDVLMDIQAVGRIDDYNSIRDLQTMNMSPLSETNDAVVLLKDFRPSPVSVTYEWXXXXXXXXXXXXXXXXXXXXXXXXXXXXQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CM12</accession>
    <name>A0AAD5CM12_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GIL1/IRKI C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_014217</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007815">
        <property type="protein sequence ID" value="KAI7743074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CM12"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056813">
        <property type="entry name" value="GIL1_IRKI_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042316">
        <property type="entry name" value="IRKI-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31029">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31029:SF4">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24994">
        <property type="entry name" value="GIL1_IRKI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GIL1/IRKI C-terminal" evidence="3">
        <location>
            <begin position="409"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="176"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="37"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="64"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="81"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7743074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="474" mass="53431" checksum="DAD8C420E8C6D3D9" modified="2024-05-29" version="1">MMAASLCSSSSISTSFSPHLRRYPPFTPIQEEDEFSDQNSVKATPTTDCSETTKDSYYVHQPTPLHQTSKPTVKSSKKKKSESESKPDDNRTVSCNKCRPSVREKFSVVPLDTTTVGRHSISSPNALLKSFFTSIIKKSPKSTSSSSSSDALNQEDQWKAAVAELSHKLIHATKKRDEALLEASRLKSSMTELETKLNKLEIYCHELKSGLDHCANKAGNIYGHVKINDHDKVIENFLVSVSESRSTMRHLSRSLTVQLQQMSGGKLYDRIQVLVRPYDIKISGTTNPRIVLLYLEALLNGAFFEDFESVGFQKSGSNRVLNPIHKCEENFKCFMRLKELSWDEVLNKGTKQFSEEFSKFCDRKMSEVVSMLGWTRAWPEPLLQAFFAASKAVWLVHLLANSVHPGLPVFRVDKGVRFDSIYMEDMGGDKAKRFASPTVRVMVEPGFYIYGNVVKCKVICRYNNNNVFLNGSPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CNZ8</accession>
    <name>A0AAD5CNZ8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_001239</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007474">
        <property type="protein sequence ID" value="KAI7744630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CNZ8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12325">
        <property type="entry name" value="RRM1_hnRNPA_hnRNPD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12330">
        <property type="entry name" value="RRM2_Hrp1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000051">
        <property type="entry name" value="Heterogeneous nuclear ribonucleoprotein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000102">
        <property type="entry name" value="Heterogeneous nuclear ribonucleoprotein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48032:SF6">
        <property type="entry name" value="RNA-BINDING (RRM_RBD_RNP MOTIFS) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48032">
        <property type="entry name" value="RNA-BINDING PROTEIN MUSASHI HOMOLOG RBP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="7"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="111"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="285"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="290"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7744630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="472" mass="49996" checksum="2943648463CF4A09" modified="2024-05-29" version="1">MEPDNGGKLFIGGISWDTDEERLKEYFGSYGEVSEAVIMRDRLTGRARGFGFVVFVDPAVAERVVMEKHMIDGRTVEAKKAVPREDHHGLGLNRTINSIQGSPAGPMGRTKKIFVGGLPSNITESDFKSYFDQFGTIADVVVMYDHNTQRPRGFGFITFDSEEAVDRVLYKTFHELNGKMVEVKRAVPKELSPSPSRSPLIGYNYGLNRPNTFLTSSYPQGYNMGPIGSFGVRMDSRFSPTVGGRTGFPQFNSPGYGMGLNMEPALSPTAFGGSSGYGRVLSPHFGGNPNRYNTPIGYNQGSGRTEPPTSSSSRNVWGNGGLTSPIGSGSYLGSGSGGTGFGVFGNNGPSWGSLALNSQNGGSSSGYNGGSLGGFRGEENSYGIVGRNSVAGVATSSVNGASGGHEGFYGNLYRGGSIYGDATWQSSSNEVDGSGSFGDVLGNAEGVVDRSSEEYVGNYSVANRQTSREIAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CPF4</accession>
    <name>A0AAD5CPF4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_020804</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007572">
        <property type="protein sequence ID" value="KAI7744186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CPF4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039624">
        <property type="entry name" value="LEA1/2/D7/KIN2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34191:SF23">
        <property type="entry name" value="ABA-INDUCIBLE PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34191">
        <property type="entry name" value="LATE EMBRYOGENESIS ABUNDANT PROTEIN (LEA) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7744186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="67" mass="7279" checksum="419FB8A58EDBCD2C" modified="2024-05-29" version="1">MKSSQSPAEQETARAKAEAQEKRRELMEQASNAAQSAKDSMQQAGQQIKEKAQVATEAVKNATGMKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CPK9</accession>
    <name>A0AAD5CPK9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BEACH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_027081</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007403">
        <property type="protein sequence ID" value="KAI7744960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CPK9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1540.10">
        <property type="entry name" value="BEACH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000409">
        <property type="entry name" value="BEACH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036372">
        <property type="entry name" value="BEACH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46866">
        <property type="entry name" value="GH12955P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46866:SF1">
        <property type="entry name" value="GH12955P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02138">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01026">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81837">
        <property type="entry name" value="BEACH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50197">
        <property type="entry name" value="BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="BEACH" evidence="4">
        <location>
            <begin position="1"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="944"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1122"/>
            <end position="1155"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7744960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="1276" mass="142292" checksum="5C0DE59812FBB186" modified="2024-05-29" version="1" fragment="single">MPWVIDFSVKPDENNEAGWRNLSKSKWRLAKGDEQLDFTYLTSEIPHHISDECLSELAVCSYKARRLPLRVLRSAVRSVYEPNEYPSTMHRLYQWTPDECIPEFYYDPKIFHSQHSGMSDLAVPSWASSPEEFIKMHRDALESHRVSCQIHHWIDIIFGYKMSGEAAISAKNVMLPPSEPTNARSAGRRQLFSLPHPARWGVIKKSSHHNLDAVTGEAHLLHETSNLKALEKAAMFIEHASHLSPVYNPGLEESLSQEEVTSETFEYGRDTNLPLAVDINFLLQNIEADDCSTSGYQDFLLWRQKLSSSTNHSAKIANDVFSIGCILAELHLRRPLFNPASLSQYLHCGVLPKLMHELPPQVNILVEVCIQKDWKRRPSIKALLESPYFPATVRSSYLFLAPLQLLSKDGSRIHYVSSYAKSGALKLMGSFAAEMCAPHCLPLLLTPLSDTEAECAYIILKEFLKCLNLQAVKTLLLPVIQRILQTTGYSHMKVSLLQDSFVRELWSKVGKQVYLENIHSMVLSNLYVSPHKSSSGAASVLLIGSTDELGVPLTVHQTILPLVQCFGKGLCSDGIDVIVRIGGLLGETFIVKQILPLLKNVVRSCIDASNVSRPEPMQSLSALALMDCLMTLDGIVELLSSEVIVKELIEDGGCLYIQLLMTSKLGMPSLQVGATSLVAVSQLIGPELTTLYVMPKLKELFEKLAFSEETRSGPSQKVAKNKVENEQMENRMDLVFVLFLSLFVLLLYPPFASLLGIETLRQCCTTWLILEQYLLWNHNWKWEKTEESSRGGVDNINARRTNYTKNPTPEYNSASAKVFLHGFGWSVPQSQVNKGVKKSISHKRHHHVTSSSMTKEQEPWHWFPSPATSWDGPDFLGRVGASSNEIPWKISASVIHSVRAHHGALRSFAVCQDECTFFTAGVGPGFKGSVQKWDLSRIVSSSAYDGHEEVVNDICVIGSYERTASCDGTIHLWNSQSGKKISVIAEDSVQYGNSSMSTSKIHSDQANMLDFSSLGSGILSSTYDGSMYTCMHHLETVNRLVAGTGNGSLRFIDIEEGQKLHLWRSDSIETSFPSLISSICSCGSTKMQANGAASSPSWIAAGLSSGHCRLLDMRSGDLITSWQAHDGYVTKLAAPEDHLLVSSALDKTLRVWDLRKNLASPLILFRGHSDGVSGFSVWGQDVISISRKCVAHIYIYIYIYGINGKCDVIAQDGQHRITPQYLYMADRESRNMSVLSTITILPFSRLFLVGTEDGYLKILFNMMINQQAGDIHKKKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CQ67</accession>
    <name>A0AAD5CQ67_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_016239</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007018">
        <property type="protein sequence ID" value="KAI7746161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CQ67"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36078">
        <property type="entry name" value="BNACNNG21220D PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36078:SF2">
        <property type="entry name" value="OS09G0473966 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7746161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="111" mass="12695" checksum="4C39146E2A67BF91" modified="2024-05-29" version="1" fragment="single">SLIRRLVATVTTLHFREMDNKEQSENLKNCEARFAGYLKAKYFSDKDIYGGEIFEEKSIINGMTIRASSEAGTRSYADPFTYWNEKFARKETKTETNANLSNGKHSSHKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CQD6</accession>
    <name>A0AAD5CQD6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_003733</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007049">
        <property type="protein sequence ID" value="KAI7746057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CQD6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10555:SF170">
        <property type="entry name" value="FI18122P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10555">
        <property type="entry name" value="SORTING NEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PX" evidence="1">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7746057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="103" mass="11616" checksum="6A830E47057A510F" modified="2024-05-29" version="1" fragment="single">YQGSEKIVIRRYTDFVWLRDRLFEKYKGVFIPPLPKKSTVEIGDISTYTSSSESQAKYISRANGVIAAANTAAEVTLRVVKPGKKIYRCQTTNITLLKVAKTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CQS6</accession>
    <name>A0AAD5CQS6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein ABHD18</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_020956</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007435">
        <property type="protein sequence ID" value="KAI7744816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CQS6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019149">
        <property type="entry name" value="ABHD18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13617">
        <property type="entry name" value="PROTEIN ABHD18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13617:SF14">
        <property type="entry name" value="PROTEIN ABHD18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09752">
        <property type="entry name" value="ABHD18"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7744816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="357" mass="39704" checksum="E9F837939B35C3BD" modified="2024-05-29" version="1">MVAIKIGMLHYVLDHVYGAFMHRTKLSPPFFSRGWGGSKLDLLENMIKQIFPAAEGQNWPPRLIQPMWKTVWETKNACLKEGLFRTPCDEQIINALPPESHVARVAFLAPKFVPPHKMACVVHLAGTGDHSFERRLRLGGPLLKENIATMVLESPFYGQRRPFLQSGSKLLCVSDLLLLGRTTIEEARSLLHWLDSEAGYGKMGVCGLSMGGVHAAMVGSLHPTPIATLPFLSPHSAVVAFCEGVLKHATAWDALRQDLSTAMTLHEVQEHMRHVLSLTDVTRFPIPKNPNACILVAATDDGYIPKHSVLEVQRAWPGSEVRWVTGGHVSSFILHNGEFRKAIMDGLNRLKWKEPPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CQW1</accession>
    <name>A0AAD5CQW1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-directed DNA polymerase family A palm domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_029872</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006943">
        <property type="protein sequence ID" value="KAI7746566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CQW1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001098">
        <property type="entry name" value="DNA-dir_DNA_pol_A_palm_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002298">
        <property type="entry name" value="DNA_polymerase_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10133">
        <property type="entry name" value="DNA POLYMERASE I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10133:SF62">
        <property type="entry name" value="DNA POLYMERASE THETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00476">
        <property type="entry name" value="DNA_pol_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA-directed DNA polymerase family A palm" evidence="1">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7746566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="101" mass="10912" checksum="F888394F6891FFEF" modified="2024-05-29" version="1" fragment="single">GSAADIIKVAMITIHSVVGDGIEKSKSSVEFAERFHMLKGRCRILLQVHDELILEADPSVMNEAGMLLKLSMESAASLLVPLIVKLKCGRTWGSLEPLLPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CRZ7</accession>
    <name>A0AAD5CRZ7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_002020</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006813">
        <property type="protein sequence ID" value="KAI7747228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CRZ7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071818">
        <property type="term" value="C:BAT3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051787">
        <property type="term" value="F:misfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031593">
        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15204">
        <property type="entry name" value="LARGE PROLINE-RICH PROTEIN BAG6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15204:SF0">
        <property type="entry name" value="LARGE PROLINE-RICH PROTEIN BAG6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="82"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="181"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="200"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7747228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="281" mass="29931" checksum="793314A7FDCD297D" modified="2024-05-29" version="1" fragment="single">SVLAPAVNQREASGGERPTVSDGGISNHQAAPRNMSQPTGASDVRVVPIRISTLPPGLAPSESSLGSVSVLYPVVARNDINGHVSNGSQTHNTENRPHLVPNSTAQQQPPEVPGTTHGSLHSGQTFSTEIPSGLDQLLRNLFPDVDTAPAPNFTQETPAVSDEGAFLSNLLHQIMPIVTQHLNGGADGENGATQPPSNVEENHDRGASSRQQDDPSSPPSSKRQKALTLALSLPCESGVHHCPYDLRFDRVSYCKSKLKPRKEQKKWVSATDIKIPILGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CS68</accession>
    <name>A0AAD5CS68_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_002726</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein disulfide isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007183">
        <property type="protein sequence ID" value="KAI7745640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CS68"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003756">
        <property type="term" value="F:protein disulfide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034976">
        <property type="term" value="P:response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18929">
        <property type="entry name" value="PROTEIN DISULFIDE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18929:SF246">
        <property type="entry name" value="PROTEIN DISULFIDE ISOMERASE-LIKE 1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7745640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="109" mass="12536" checksum="A78408BE25B7BCAD" modified="2024-05-29" version="1">MSADFKHPNFHGPAGKWKKTRSVIMNDIYITYKLIFVYVDLDNEDVGKPIADYFGITEEAPKVVGYTGNDDGKKFVFDQDLTLENRKIFGEDFLKDKPKPLYKSDPIPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CUG8</accession>
    <name>A0AAD5CUG8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Branched-chain-amino-acid aminotransferase</fullName>
            <ecNumber evidence="9">2.6.1.42</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_010284</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-isoleucine + 2-oxoglutarate = (S)-3-methyl-2-oxopentanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24801"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:35146"/>
            <dbReference type="ChEBI" id="CHEBI:58045"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:18321"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:17865"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-valine + 2-oxoglutarate = 3-methyl-2-oxobutanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24813"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57762"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.42" evidence="9"/>
    <dbReference type="EMBL" id="JAMZMK010006727">
        <property type="protein sequence ID" value="KAI7747570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CUG8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004084">
        <property type="term" value="F:branched-chain-amino-acid transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009082">
        <property type="term" value="P:branched-chain amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.10.10:FF:000003">
        <property type="entry name" value="Branched-chain-amino-acid aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.10.10">
        <property type="entry name" value="D-amino Acid Aminotransferase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001544">
        <property type="entry name" value="Aminotrans_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018300">
        <property type="entry name" value="Aminotrans_IV_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036038">
        <property type="entry name" value="Aminotransferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005786">
        <property type="entry name" value="B_amino_transII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043132">
        <property type="entry name" value="BCAT-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043131">
        <property type="entry name" value="BCAT-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42825">
        <property type="entry name" value="AMINO ACID AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42825:SF29">
        <property type="entry name" value="BRANCHED-CHAIN-AMINO-ACID AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01063">
        <property type="entry name" value="Aminotran_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006468">
        <property type="entry name" value="BCAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56752">
        <property type="entry name" value="D-aminoacid aminotransferase-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00770">
        <property type="entry name" value="AA_TRANSFER_CLASS_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="3 9" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="9" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="5 8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="6">
        <location>
            <position position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR006468-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7747570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="329" mass="36001" checksum="DD62F3790FD31BA1" modified="2024-05-29" version="1">MIRQCSFLRRFVQISSSKVKGRCLTTHVTSTLQETEPMARYEEYADIDWNNLGYGLIKTDYIYKAKCTLNNTFEQGQVCDYGNIELSPGAGVLNYGQVPKLLGEKTIPPPGKGSLYIRPLLIGTGPTLGVSPAPEYTFLVYASPVGNYFKAGTGPLNLYVNSEFHRATRGGAGCVKSITNYAPVLKAISGAKEQGFSDVVFLDSVHKKYIEEASCSNIFLVKGNVISTPSTVGTILEGITRKSIIDIARDLGYKVEERLIALDELMEADEVFTTGTAVSVAVVGSVTFNAQRIEYKTAGELVSQKLYKTLVGIQVGKIKDKYDWAVEIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CVS3</accession>
    <name>A0AAD5CVS3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UspA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_000359</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006571">
        <property type="protein sequence ID" value="KAI7748245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CVS3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23659">
        <property type="entry name" value="USP_At3g01520-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000206">
        <property type="entry name" value="Universal stress protein family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46100">
        <property type="entry name" value="IMP2'P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46100:SF4">
        <property type="entry name" value="USPA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="1">
        <location>
            <begin position="4"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7748245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="159" mass="17255" checksum="6C207E56A46D452D" modified="2024-05-29" version="1">MGEKRIGVAVDFSVGSRAALQWAIDNVVRKGDHLILVTIRPEGDSEAQLWETTGCPLIPLSELCDAQVMKKYGAKPDSETLDIVNLAATQKEVVVVMKIFWGDPREKLCEAITNIPLDHVVIGNRGLGKLKRAIMGSVSNYVVNQSSCPVTVVKHASST</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CYH0</accession>
    <name>A0AAD5CYH0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Importin subunit beta-1/Transportin-1-like TPR repeats domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_028316</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006302">
        <property type="protein sequence ID" value="KAI7749707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CYH0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058584">
        <property type="entry name" value="IMB1_TNPO1-like_TPR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25574">
        <property type="entry name" value="TPR_IMB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Importin subunit beta-1/Transportin-1-like TPR repeats" evidence="2">
        <location>
            <begin position="40"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7749707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="108" mass="11952" checksum="F7C6B479CFCFD9B0" modified="2024-05-29" version="1">MCLGSLNPLRVCFCNQVINVAGWTTPNSAKTRGFQINLRIFQGFKNSPKTQLLIPYAPHILQFLDLIYMEKDMDEVVMKIAVGVLGDLDTLGSYGGPLFNSLFGAKIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CZL9</accession>
    <name>A0AAD5CZL9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TPX2 central domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_007312</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006237">
        <property type="protein sequence ID" value="KAI7749974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CZL9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005880">
        <property type="term" value="C:nuclear microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030295">
        <property type="term" value="F:protein kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090307">
        <property type="term" value="P:mitotic spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060236">
        <property type="term" value="P:regulation of mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027330">
        <property type="entry name" value="TPX2_central_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009675">
        <property type="entry name" value="TPX2_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14326:SF15">
        <property type="entry name" value="OS06G0130200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14326">
        <property type="entry name" value="TARGETING PROTEIN FOR XKLP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12214">
        <property type="entry name" value="TPX2_importin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TPX2 central" evidence="2">
        <location>
            <begin position="246"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="129"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7749974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="574" mass="64039" checksum="81DE3AB3A6977993" modified="2024-05-29" version="1" fragment="single">MEVEMDMDDDNSSIDVEFTFTAVEIDIDYEFEAARFFDFTRMETVDEAREAEMWFDSVGSYPPSLRLLSRGKDENANVSTVSKGLDNANQLDSASDRAPEAFTMAMSNTDGDVTNGGVPLELKGYSLQASQKQRTNSFIPKDSTRNANCKSKPKSSWKPSFPRASTLMKPTASQLAKQNQERLIDHFRFQKPGNNSSVVESQAAKRQKLEGGHLFKITDTKQEANFVHKAPKREGTLDSNLGHGRLRITVPRPPDLATAQRAQRIRQKGDNENAHVASRAPGFRARPLNRKIFEAPSLLHQKRSTPQLPEFQEFHLKTTERAVHNTAAVPSTSACCNNLKAPLKPSLAFGTESSGRESKGSHVSAVLKQEDCETIHRFKALPLNKKIFSSRGDLGVFRSSKRETTVATAFNFQTEKRAQHAPPVDLFNKLSLASESGSRSSRAPSIFTKGSKENRVCSFQQQSAVKIPKPGSKQKLGNPLKVCNLVPLLNQCRALLVLADLISHCRLYHTTVNVLIKPSKRQVVQLLPSDIKRKIKIALKWYQKILLEGCPTNRKTTLRWAGEWAPTLAREQAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D143</accession>
    <name>A0AAD5D143_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HMA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_032470</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005964">
        <property type="protein sequence ID" value="KAI7751140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D143"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009626">
        <property type="term" value="P:plant-type hypersensitive response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044258">
        <property type="entry name" value="HIPP09-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47066">
        <property type="entry name" value="HEAVY METAL-ASSOCIATED ISOPRENYLATED PLANT PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47066:SF1">
        <property type="entry name" value="HEAVY METAL-ASSOCIATED ISOPRENYLATED PLANT PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMA" evidence="3">
        <location>
            <begin position="46"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="3">
        <location>
            <begin position="134"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="196"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="202"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="215"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7751140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="304" mass="34131" checksum="08171C7AC75CD864" modified="2024-05-29" version="1">MGEEQKVEEPKAEEKKEDVKPEEKKEEKGEEKPPAEEKKDEEPKPPPPFVLHIDLHCVGCAKKIERSLLRIPGVVGIDINMEKNQVTIKGVVEPQAVCDKIAKKTKRTVKVLSPLPAAEGEPIPQVVTSDVPESITVELNVNMHCENCAQQLKRKILRMKGVRTVETEVSASKVTVTGSMDGNKLVEYVYKRTKKQAKIVPQPEPEPAPAPAPEPAEKPKEEAKAEEKPAEEVKAEEKPAEEEKKEGGGEAKEEEGKVEKVERMEEIDMQRMMYYHQYPPMYVMERIPPPQLFSDENPNACSIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D186</accession>
    <name>A0AAD5D186_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_021284</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005949">
        <property type="protein sequence ID" value="KAI7751215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D186"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000013">
        <property type="entry name" value="TBC1 domain family member 8B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000018">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF20">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="3">
        <location>
            <begin position="188"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="51"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="122"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="220"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="673"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="717"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="569"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="60"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="126"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="220"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="673"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="720"/>
            <end position="758"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7751215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="774" mass="87751" checksum="3444E47383058E60" modified="2024-05-29" version="1">MEILLDRDAYGFAVRPQHVQRYREYATIYKEEEEERSDRWKDFLERPSEFVKLPVNDSPSQEHKADSLDADSEKAPEGDDSKEPEAQTTHQAQIWAQIRPSLRAIEDMMNARVKKKIIATKSEQDAGAEEDSEDEFYDMERSESDPIPDVPSSVGVSVPASGADSNPTVSLESSVLWKQELEFLVQGGVPMALRGELWQAFVGVKARHIKNYYQNLLLPDNKKDNSDDHQKAEVDEANNKSTVDSIDVPEKWKLQIEKDLPRTFPGHPALDEGGRNALRRLLTAYARHNPSVGYCQAMNFFAGLLLLLMPEENAFWALLGILDDYFEGYYSEEMIESQVDQLVFEELVRERFPKLVNHLDYLGVQIAWVSGPWFLSIFMNMLPWESVLRVWDVLLFQGNRVMLFRTALALMELYGPALVTTKDAGDAVTLLQSLAGSTFDSSQLVLTACMGYQNVNEERLQELRNKHRPAVEAALEERTKGLNMWRDSQGLASKLYGFKENKTENKITNGGLSRMSSETSSVDELCLEVNSVPDLPEQVARLKGELCKVLEEKRSAIIRAEELETALMEMVKQDNRRELSAKVEQLERDVAELQQALADKQEQENAMLQVLMRVEQEQKVTEDARRYAEQDAAAQRYAIEVLQEKYETATASLTEMEKRVVMAESMLEATLQYQSGQTKAQPSPRATKQDSSTIRTSQELSPEIPARKISLLSRPFGLGWRDKNKADVKATSEEQSPKPTEEQTDIKVTGDEPTAKDDENVDQMENVSLVDDGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D275</accession>
    <name>A0AAD5D275_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_015874</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005665">
        <property type="protein sequence ID" value="KAI7752523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D275"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7752523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="119" mass="13016" checksum="B6A31E27B902D88E" modified="2024-05-29" version="1">MKLHQIRSLLLNIKDPVHIPVSIKGGRVNGRSKVTKNNKAAPRTPVSNSGLLSPLTPASNCCYDSSIDVILDLNNAADILEVIDASKPEGMDDGVTLLQAEVQRLSMEENRLDENIRKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D2S9</accession>
    <name>A0AAD5D2S9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">P-type ATPase A domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_023405</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIIA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005538">
        <property type="protein sequence ID" value="KAI7753128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D2S9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000045">
        <property type="entry name" value="ATPase 4 plasma membrane-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000191">
        <property type="entry name" value="Membrane H(+)-ATPase1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000211">
        <property type="entry name" value="Plasma membrane ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42861">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00120">
        <property type="entry name" value="HATPASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="317"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="382"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="460"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="493"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="12">
        <location>
            <begin position="47"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="624"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7753128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="624" mass="69137" checksum="C85CD8FE4438AF81" modified="2024-05-29" version="1" fragment="single">GKPPDWQDFVGIITLLIINSTISFIEENNAGNAAAALMARLAPKAKSLPVTKGPGDGVYSGSTCKQGEIEAVVIATGVHTFFGKAAHLVDSTNQVGHFQKILNLAHNKSDIERRVHAVIDKFADRGLRSLAVAYQDVPDGRKESPGGPWQFIGLMPLFDPPRHDSAETIRRALNLGVNVKMITGDQLAIGKETGRRLGMGTNMYPSSALLGQNKDESIAALPIDELIEKADGFAGVFPEHKYEIVKRLQARKHICGMTGDGVNDAPALKKADIGIAVADATDAARSASDIVLTEPGLSVIISAVLTSRAIFQRMKNYTIYAVSITIRIVLGFMLLALIWKFDFPPFMVLIIAILNDGTIMTISKDRVKPSPLPDSWKLAEIFTTGVVLGSYLAMMTVIFFWAAYKTDFFPRTFGVPTLEKTAHDDFRKLASAIYLQVSTISQALIFVTRSRSWSFVERPGWLLVIAFAIAQLVATLIAVYANWSFAAVEGIGWGWAGVIWLYNIVFYFPLDIIKFFIRYALSGRAWDLVIERRIAFTRQKDFGKEQRELQWAHAQRTLHGLEVPDTKMFGDRTNVTELNQMAEEAKRRAEIARLRELHTLKGHVESVVRLKGLDIETIQQSYTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D3R4</accession>
    <name>A0AAD5D3R4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Dehydroascorbate reductase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_028153</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-dehydroascorbate + 2 glutathione = glutathione disulfide + L-ascorbate</text>
            <dbReference type="Rhea" id="RHEA:24424"/>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58297"/>
            <dbReference type="ChEBI" id="CHEBI:58539"/>
            <dbReference type="EC" id="1.8.5.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the GST superfamily. DHAR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005936">
        <property type="protein sequence ID" value="KAI7751295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D3R4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045174">
        <property type="term" value="F:glutathione dehydrogenase (ascorbate) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140547">
        <property type="term" value="P:acquisition of seed longevity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033355">
        <property type="term" value="P:ascorbate glutathione cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03201">
        <property type="entry name" value="GST_C_DHAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00570">
        <property type="entry name" value="GST_N_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000102">
        <property type="entry name" value="Glutathione S-transferase DHAR3 chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000029">
        <property type="entry name" value="Glutathione S-transferase DHAR3, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044627">
        <property type="entry name" value="DHAR1/2/3/4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44420">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE DHAR2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44420:SF2">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE DHAR2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13410">
        <property type="entry name" value="GST_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13409">
        <property type="entry name" value="GST_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0216">Detoxification</keyword>
    <keyword evidence="4" id="KW-0318">Glutathionylation</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="8">
        <location>
            <begin position="10"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="9">
        <location>
            <begin position="66"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7751295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="214" mass="23926" checksum="CE8ACCDE96CCF7E4" modified="2024-05-29" version="1">MAIEICIKAARGAPDVLGDCPFSQRIRLTLEEKKIPYKTHLINLDNKPKWLFEVSPEGKVPLAKFDDNWVSDSDVIVGIIEDKYPHPSLVTPPELASVGSNIFSKFIAFLKSKDANDGTEQALLDELNALNEHLKSKGPYVNGETITAVDLSLGPKLYHLQVALGHYKNWTVPESLTHLHNYMKSLFSRESFEKTKPPKEEYVIAGWAPKVNSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D409</accession>
    <name>A0AAD5D409_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF1517 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_029203</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005635">
        <property type="protein sequence ID" value="KAI7752670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D409"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010903">
        <property type="entry name" value="DUF1517"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053023">
        <property type="entry name" value="FLAP_modulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33975">
        <property type="entry name" value="MYELIN-ASSOCIATED OLIGODENDROCYTE BASIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33975:SF2">
        <property type="entry name" value="MYELIN-ASSOCIATED OLIGODENDROCYTE BASIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07466">
        <property type="entry name" value="DUF1517"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7752670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="164" mass="18638" checksum="842246E8B9C574E6" modified="2024-05-29" version="1">MPFVVMKVGSLGTVRPLQKDLNRIAKSQYWLCFGTQIIAFLVNVKRSVEECEKQFNRLSIKERVKFDEETLVNVNNIKMKSAKSQSFDGLRNDFIVVTIIVAAHGVVELPPIKSTAQLKEALQKLATIPSSKIMGVEVLWAPQKEDDSLTEQEMLEYYPLLRPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D526</accession>
    <name>A0AAD5D526_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Malectin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_006982</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005477">
        <property type="protein sequence ID" value="KAI7753300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D526"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000135">
        <property type="entry name" value="Putative LRR receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.430">
        <property type="entry name" value="Galactose-binding lectin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024788">
        <property type="entry name" value="Malectin-like_Carb-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45631:SF191">
        <property type="entry name" value="DI-GLUCOSE BINDING PROTEIN WITH LEUCINE-RICH REPEAT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45631">
        <property type="entry name" value="OS07G0107800 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12819">
        <property type="entry name" value="Malectin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="614"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="domain" description="Malectin-like" evidence="3">
        <location>
            <begin position="95"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7753300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="686" mass="75695" checksum="2E5F72F698068656" modified="2024-05-29" version="1">MEYNFNKYRQTLEVTSPPNSLKILTTIQTHLSLTPFIHSTSVLILSPLIHHHFFLQSSPSSPHHTMPPLTTLFLLLFLFLFHPSSSFPFDISYHIDCGSTTTTTDKYNTTWLSDRFFTSGSTGLVSEPLLFHSFHQKTLRYFPVSSGKKNCYIIPNLPVGKYHLKTFTVYDNYNGKHHAPSFDMSVEGTLVSSWRSPWEQQTSRYGDNKDVVFFVSDGHADVCFYSIATDSPVIGSIQLFQIDPDSYEPGSTGKESVLVSYGRLSCGSSRWGPGFSNDVDVFGRVWESDVKYRLKSMVGVKSVTAVKKVGNSGIGPNYYPGKLFESAVTLTGTGDLEYELPVDAKLDYFVWLHFAEIDVSVDRKGKRVFDVVINGENVTRIDIFKEVGGFAAYSYRYIARNLSGSPLSVKLVHVVGSPLICGLENYAIVLADVMTVPEQVIAMQALKESLGVPERMGWNGDPCAPTNWDSWEGVTCRPNKNESALVIFQIDLGNQGLKGYISDKIVLLTNLVTLNLSSNTLGGTLPTGLGQGSLKKLDLSNNQISGSIPISLSSSNLQLVLLNGNKLEGQVPEDLYSIGVHGGAIDLYANKGLCGVPSLPDCPLFSGLSHGGKIAIVVSCGVVFLLVLVLIFICKRRRRNDYDFALPHELVSLAAKKNKYQTQKSLMALEMESQHAKGYMPNYNSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D566</accession>
    <name>A0AAD5D566_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_015732</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005790">
        <property type="protein sequence ID" value="KAI7751925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D566"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1170.10">
        <property type="entry name" value="DNA repair protein MutS, domain I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016151">
        <property type="entry name" value="DNA_mismatch_repair_MutS_N"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7751925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="177" mass="19692" checksum="A611AC22631D4011" modified="2024-05-29" version="1" fragment="single">MGVVSTVHMTLFSASMDGGGVGEAPNELYVAYVNAQKPFNRKSLSRIFEEQNISNNHQGKSYLLYELDAEIGHKELDVAMSGSEKCIQLSISETAIDGSIEKLVARRIFFIKKRQINFQMAQLPEGITELLEHEKLPVPLGSKKRDEKMQQQEGAGLDFLSNHGHVEKMEILSGIAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D5C4</accession>
    <name>A0AAD5D5C4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein 18</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_006708</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TMEM18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010004039">
        <property type="protein sequence ID" value="KAI7754233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D5C4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026721">
        <property type="entry name" value="TMEM18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22593">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22593:SF2">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14770">
        <property type="entry name" value="TMEM18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="50"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="74"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="124"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7754233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="163" mass="18988" checksum="A66D6B656ABBCF01" modified="2024-05-29" version="1">MDELKTAMNDHLDQMQEIVEKISSELRVGMQPAMDNFIGFFHAIDWKEPWLVGLLVFHFMVLIIILMSRKNINFQMYLFLLSLAGVYLAERLNTVLSENWKSFAGQNYFDRHGVFLSVLWSGPLLVFATIILVNTLFSLCHLIVKWKRAELKHRARLSATKQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D5H0</accession>
    <name>A0AAD5D5H0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNA mismatch repair proteins mutS family domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_012330</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA mismatch repair MutS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005511">
        <property type="protein sequence ID" value="KAI7753199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D5H0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032301">
        <property type="term" value="C:MutSalpha complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000432">
        <property type="entry name" value="DNA_mismatch_repair_MutS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045076">
        <property type="entry name" value="MutS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361:SF148">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MSH6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MUTS FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00488">
        <property type="entry name" value="MutS_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00534">
        <property type="entry name" value="MUTSac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00486">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041959617" description="DNA mismatch repair proteins mutS family domain-containing protein" evidence="5">
        <location>
            <begin position="18"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="DNA mismatch repair proteins mutS family" evidence="6">
        <location>
            <begin position="36"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7753199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="148" mass="16174" checksum="1078AACD8BB34A94" modified="2024-05-29" version="1" fragment="single">VLVICLLLNSPTLFVGTFLIECTETASVLQNASQDSLVILDELGRGTSTFDGYAIAYAVFRHLVDKVNCRLLFATHYHPLTKEFASHPHVTLQHMACTFKPISDNLTDQQLVFLYRLAAGACPKSYGMQVALMAGIPKKVVESATKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D6N0</accession>
    <name>A0AAD5D6N0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">M8C21_016967</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">AA19_3_7</strain>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the pex2/pex10/pex12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005657">
        <property type="protein sequence ID" value="KAI7752555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D6N0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016558">
        <property type="term" value="P:protein import into peroxisome matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16526">
        <property type="entry name" value="RING-HC_PEX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025654">
        <property type="entry name" value="PEX2/10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006845">
        <property type="entry name" value="Pex_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045859">
        <property type="entry name" value="RING-HC_PEX2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48178">
        <property type="entry name" value="PEROXISOME BIOGENESIS FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48178:SF2">
        <property type="entry name" value="RING-TYPE E3 UBIQUITIN TRANSFERASE (CYSTEINE TARGETING)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04757">
        <property type="entry name" value="Pex2_Pex12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="16">
        <location>
            <begin position="286"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAI7752555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="341" mass="39112" checksum="A3F2070AEFC451F7" modified="2024-05-29" version="1">MRETIASTSTASNSVPPAESAWITTCERVSPRWQSFALPPQAAIPIVISRVNQVDAARLDVEMSAMLKEQLVKVFSVMKPGLLFQYEAELDAFLEFLIWRFSIWVDKPTPGNALMNLRYRDERANEMREKVRTGLEGPGLTMAQKIWYCIATVGGQYIWTRLQSFSAFRRWGDSEQRSVANRAWYIIQRIEGFYKAASFTNLLIFLYTGRFRNLIERAIRARLVYGSPHMNRAVSFEYMNRQLVWNEFSEMLLLLLPILNSASIKSLFRPFSKDKSSGSTGDETQCPICHINPTIAYVALPCQHRYCYYCLRTRCAATTSFRCARCSEPVVAMQRHGSINT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D786</accession>
    <name>A0AAD5D786_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_010920</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005600">
        <property type="protein sequence ID" value="KAI7752840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D786"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34776">
        <property type="entry name" value="F17F16.3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34776:SF1">
        <property type="entry name" value="F17F16.3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7752840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="272" mass="31271" checksum="CDFE7ABDF8E1FF92" modified="2024-05-29" version="1" fragment="single">MAPPGEEENTPEDVEIQEKGDIYFFYRPKVGNEEPHSSDDVQRLYIVLRPHQEKQPPQEEKDEEKSMLLRLILMGKKSLPDPAKRSRPYWGFVELVTTKIQDVKDALQAEEYDTKTKGHRHKPAARALGEGVYRILKHHSRKKMHTHLVYKLEFPSEGAQNQPQEELNVEREGSFLIQIKNPTQGQGQKKTKKKKAIFPAQLQGQFGKLRYHVADPTEFLNYEGCEFLLISASDDIHKELGLEMIEQDVSCSDLVNTFGETVSADALFRGIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D7Z2</accession>
    <name>A0AAD5D7Z2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Transcription factor MYB44-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_007693</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000621">
        <property type="protein sequence ID" value="KAI7756046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D7Z2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000060">
        <property type="entry name" value="MYB transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000344">
        <property type="entry name" value="Transcription factor MYB44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050560">
        <property type="entry name" value="MYB_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614">
        <property type="entry name" value="MYB PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614:SF293">
        <property type="entry name" value="TRANSCRIPTION FACTOR MYB73"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="7"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="7"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="59"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="59"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="182"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7756046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="269" mass="30000" checksum="279AA8C9648D4C83" modified="2024-05-29" version="1">MVVVKKDVDRIKGPWSPEEDMLLQTLVQRHGARNWSLIGSSIPGRSGKSCRLRWCNQLSPQVEHRAFTPEEDETILRAHAQFGNKWATIARLLNGRTDNAIKNHWNSTLKRKCSSMSNDDFSFLDEHDRLNYQPALKRCASLGPGPGCAEFLHNPASPTRSETSDSRRLDLNDLHTSLSLSLPGYEVKQKSPEATPVTTQQQRPPTPPLAVPGGDGGNSGFLNPELLAVMHEMIKTEVRQYYMEKGQCLQAESAISNAVINRMGMNRIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D7Z6</accession>
    <name>A0AAD5D7Z6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphoribulokinase/uridine kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_003722</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010003020">
        <property type="protein sequence ID" value="KAI7754594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D7Z6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006083">
        <property type="entry name" value="PRK/URK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10285">
        <property type="entry name" value="URIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00485">
        <property type="entry name" value="PRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphoribulokinase/uridine kinase" evidence="1">
        <location>
            <begin position="87"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7754594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="270" mass="30180" checksum="B4D9D1E42946950F" modified="2024-05-29" version="1" fragment="single">MVLLKKVGVSQCYQGSAFMFSNKSRSQRSLNQSVSLRHKKCPVQVLCSQKKDIPILEARSMDEIYDTLAERLVPTAASGTNTSLTHIVGLAGPPGAGKTTIALEVAKRVNKLWPQKASGFDSQVQPPEIAVVLPMDGFHLYRHQLDSMEDPKEAYARRGAPWTFSPELFLKCLKTLREQGSVYAPSFDHAVGDPIEDDIYINVQHKVVIVEGNYLLLDEGSWKEISSMLDEKWFVDVDIDIAMERLLERHISTGVGSTPEIAKQRFYWGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DAG9</accession>
    <name>A0AAD5DAG9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">CRC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_023999</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the lin-54 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000551">
        <property type="protein sequence ID" value="KAI7756152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DAG9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005172">
        <property type="entry name" value="CRC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033467">
        <property type="entry name" value="Tesmin/TSO1-like_CXC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044522">
        <property type="entry name" value="TSO1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46159">
        <property type="entry name" value="PROTEIN TESMIN/TSO1-LIKE CXC 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46159:SF12">
        <property type="entry name" value="PROTEIN TESMIN_TSO1-LIKE CXC 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03638">
        <property type="entry name" value="TCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01114">
        <property type="entry name" value="CXC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51634">
        <property type="entry name" value="CRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="domain" description="CRC" evidence="6">
        <location>
            <begin position="488"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="124"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="360"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="143"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="360"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="372"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="560"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7756152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="560" mass="61029" checksum="78E4A051DD42F71A" modified="2024-05-29" version="1" fragment="single">RPSRFFFNILSSTHYLLIIIIVVQPQKQRKKVILGQMGHGKAQENEMGIVMDTPERTHVANPLSKFEDSPVFNYINSLSPIKPVKSVHFTQNFSSLNFASPPSVFTSPHVSSLKDSKFLKRHQFSDSSKPVLTSEDAKKAETSEGNLNATEDPLNQQTDFNTSNSITGTSVVPSNGCSNLESRKLNYEPVRLDTNTLKMVGPSALSAPFIDNGSENGSFRIETEAEGINELPHSKEVSTCDWDSLIADGSELLNFNSPSDTLAYTGPGQGTLYLTSLDISLMNEKPQASVDHLSENDEGLKGTAENHAVIASTSLNNLEAGEPMEDMDTEGGSNLYRGLRRRCLVFEMNGSRIKHLEDVSNASSANVSESNQTVASHDNSLRPSRNGNDSSRCNLRGIGLHLNSIASNLVDNAVKHESSGSGRQLIIGLPPAYPSMGSGQELMTTLSDSPSFENDIGPAVNVAEDASKALEFVVNDERRAENAGEPEACKRCNCKKSKCLKLYCECFAAGVYCVEPCACLECFNKPIHEDTVLATRKQIESRNPLAFAPKVIKTSDPMQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DBD7</accession>
    <name>A0AAD5DBD7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_001386</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000395">
        <property type="protein sequence ID" value="KAI7756427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DBD7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36772">
        <property type="entry name" value="SERINE/THREONINE-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36772:SF1">
        <property type="entry name" value="SERINE_THREONINE-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7756427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="222" mass="25630" checksum="D0A19948C2B84841" modified="2024-05-29" version="1">MHIIAHLFMQDPKSNSSTSRKPWYQRAMEITTPWKITYPKSPEVPTLKPIINKNLSTSTKYTTTRNTTPEQQKLRKSTSLKVATSFTRVCLCVPISSYTDVVFHAGGLPPRRSNTYPRSKPFPNCIQDCAAARTSTEGRRIFRGKSLTDDVLMRRFVIEEDAMMKERRRNEMEVMRRRNAKRRRRLGPSPLSRMVLAEEDALQNKEVSLRVVTDEFSSVEGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DEB8</accession>
    <name>A0AAD5DEB8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Carboxymethylenebutenolidase homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_032406</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dienelactone hydrolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000523">
        <property type="protein sequence ID" value="KAI7756200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DEB8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042946">
        <property type="entry name" value="CMBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002925">
        <property type="entry name" value="Dienelactn_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46812">
        <property type="entry name" value="CARBOXYMETHYLENEBUTENOLIDASE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46812:SF1">
        <property type="entry name" value="CARBOXYMETHYLENEBUTENOLIDASE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01738">
        <property type="entry name" value="DLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dienelactone hydrolase" evidence="6">
        <location>
            <begin position="126"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7756200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="342" mass="37793" checksum="7062F317257FE9C3" modified="2024-05-29" version="1" fragment="single">QHHLYDTNHEAIILPTDIYFVSFLLSNIFISSHTLLLTHTTQMGLTSAIFMASSTSLIKPPFTTFSPNLQIHKICSLSVNSGWRSSTKCRRLCCSQVRIEYGTDEEACELVNGVEVSIGEGTDTVAAYLLTAVKNNNGTGILLLSDIFGFEDSATRDFAYRIACNGYNVLLPDLFNGDPWQKERSMALFEPWLATHTQTARTNIAASIKWMVDEFVAAGISNKLGIVGFCFGGGKVIDVLAEDSDDRFGIGVSFYGTRIQPFVAAKIKVPVLYITGDDDPLCSVKVLEDIERDNECGSKLVVFKGRGHGFVHRPASNEEDKDAEEAFMIMRNWLHNGLVAEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G158</accession>
    <name>A0AAD5G158_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">S5 DRBM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_004581</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the universal ribosomal protein uS5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012171">
        <property type="protein sequence ID" value="KAI7724704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G158"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000039">
        <property type="entry name" value="30S ribosomal protein S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000002">
        <property type="entry name" value="30S ribosomal protein S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000851">
        <property type="entry name" value="Ribosomal_uS5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005324">
        <property type="entry name" value="Ribosomal_uS5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013810">
        <property type="entry name" value="Ribosomal_uS5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48432">
        <property type="entry name" value="S5 DRBM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48432:SF1">
        <property type="entry name" value="S5 DRBM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00333">
        <property type="entry name" value="Ribosomal_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03719">
        <property type="entry name" value="Ribosomal_S5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50881">
        <property type="entry name" value="S5_DSRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="S5 DRBM" evidence="10">
        <location>
            <begin position="363"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="265"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="158"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="289"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7724704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="520" mass="59809" checksum="8F04DA3EBBC138E6" modified="2024-05-29" version="1">MSTINIMSKSRVSKAILNRLKPAHSFSRTFSTSQRWQHVLPSQTRVFAVNHIESWDRNGNGNGSRLFSSMSHATGERVVRELLAEVEREKQREREQRKKAGLGTADIDAEDDEDYMGVGRLIEKLEKENQKQVDPRILNMREEPTDSESEEDDHRFTHEAIQTRQEILEKKLKRHEELLDKFTRSDNLDDAFKWMNKIDKFEEKHLALRFEYRVIGDLMNRLKEASGKDKFLLQQKLNRAIRLVKWKEAYDPNNPANYGVIQNMQSQHDPAEDSDAESKKPLIKGVSHDDDDEEEEFDEAKKTDDLVLQKLNSIDKMLEEKLAQLDHTFGRKGKVLEEEIRDLAEERNSLTEKIRRPQFRKGFDVKLIDVNRTCKVTKGGQLIKYTVLLACGNYHGVIGFAKAKGPAVPTACQKAHEKCFQNLHYVERHEDHTIAHAIQTSYKKTKVYLWPGPTQGGMKAGRTVQTILNLAGLKNVKSKVVGSRNPHNTVKALFKGLNAIETPKDVQEKFGRVVVESYLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G215</accession>
    <name>A0AAD5G215_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_015175</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012003">
        <property type="protein sequence ID" value="KAI7725217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G215"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046521">
        <property type="term" value="P:sphingoid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009305">
        <property type="entry name" value="Mpo1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28026:SF9">
        <property type="entry name" value="2-HYDROXY-PALMITIC ACID DIOXYGENASE MPO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28026">
        <property type="entry name" value="DUF962 DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_8G05310)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06127">
        <property type="entry name" value="Mpo1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="112"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="150"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7725217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="198" mass="22462" checksum="86D0821C8839318C" modified="2024-05-29" version="1">MAKIGLFDLEKHFAFYGAYHSNPVNIFIHMLFVWPILFTSLVILYFTPPLINNNNNISLPNGLVLNYGLLFTVIYAVFYVCLDVKAGSFAGLLCFVCWVFGSFVAQHLGFSLAWKVVLAAQLFCWTGQFIGHGVFEKRAPALLDNLTQAFLMAPFFVLLEALQMAFGYEPYPGFHARVKAKVDANIKEWKESKQKKKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G270</accession>
    <name>A0AAD5G270_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_020272</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011848">
        <property type="protein sequence ID" value="KAI7725830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G270"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22155">
        <property type="entry name" value="F-box_AtADLO1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46976">
        <property type="entry name" value="PROTEIN ARABIDILLO 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46976:SF1">
        <property type="entry name" value="PROTEIN ARABIDILLO 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00514">
        <property type="entry name" value="Arm"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="F-box" evidence="3">
        <location>
            <begin position="38"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="2">
        <location>
            <begin position="471"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="2">
        <location>
            <begin position="512"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="2">
        <location>
            <begin position="556"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="2">
        <location>
            <begin position="602"/>
            <end position="644"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7725830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="665" mass="72378" checksum="199AF45EE1554961" modified="2024-05-29" version="1">MMNRRVRRKCVKGAQKLDLGDQLETEDADYLKLNADGFVDWTRLPDDTVIQLFTHLNYRDRANLSSTCKSWRSLGSSSCLWQSLDLRAHKCDWGIMASLASRCSSLQKLRFRGADNADSLINLRAKSLKDLSGDYCRKLNDSTLSVIVARHKLLESLQLGPDFCERITSDAIIQIAFCCPKLKKLRLSGIRDVNKDAINALATHCPDLYEIGFIDCLKIDEMALGNVVSLRFLSVAGTTNVKWDLASESWSKLPNLKGLDVSRTDVVPNAVMRIFSSLKSLELLCAFNCPSLEEDTTVYTKSNCHGKTMLSFFNDTFTKLSPLFPNIEDERTIFSVWRNGSKKKDSNLDEFMTWVEWILSHSLLRIAESNPNGLDQFWLNQGADLLLGLLQSSQEDVQERAATGLATFVVVDDENTNVDVGRAEAVMKGGGIQLLLGLARSWKEGLQSEATKAIANLSVNPAFAKSVAGGGGITILASLAKSMNRMVAEEAAGGLWNLSVGEEHKGAIAEAGGIKALVDLIFKWPRGGDGVLERAAGALANLAADDKCSMEVASVGGINALVTLARKCKHEGVQEQAARALANLAAHGDSNTNNAAVGQEAGALDALVQLIRSQHDGVRQEAAGALWNLSFDDRNREGIALAGGVEALMNMANISDDLKKSKLEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G2D8</accession>
    <name>A0AAD5G2D8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Transcription factor-like protein DPB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">M8C21_007995</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">AA19_3_7</strain>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Involved in the regulation of the G1/S transition. Increases the DNA binding activity of E2F proteins after heterodimerization.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 11 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11 12">Belongs to the E2F/DP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011702">
        <property type="protein sequence ID" value="KAI7726339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G2D8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070176">
        <property type="term" value="C:DRM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14458">
        <property type="entry name" value="DP_DD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000187">
        <property type="entry name" value="Transcription factor-like protein DPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.80:FF:000002">
        <property type="entry name" value="Transcription factor-like protein DPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.80">
        <property type="entry name" value="Transcription factor DP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037241">
        <property type="entry name" value="E2F-DP_heterodim"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003316">
        <property type="entry name" value="E2F_WHTH_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038168">
        <property type="entry name" value="TF_DP_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014889">
        <property type="entry name" value="Transc_factor_DP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015648">
        <property type="entry name" value="Transcrpt_fac_DP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12548">
        <property type="entry name" value="TRANSCRIPTION FACTOR DP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12548:SF9">
        <property type="entry name" value="TRANSCRIPTION FACTOR DP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08781">
        <property type="entry name" value="DP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02319">
        <property type="entry name" value="WHD_E2F_TDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009404">
        <property type="entry name" value="Transcription_factor_DP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01138">
        <property type="entry name" value="DP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01372">
        <property type="entry name" value="E2F_TDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144074">
        <property type="entry name" value="E2F-DP heterodimerization region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 11 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 11 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 11 12" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="E2F/DP family winged-helix DNA-binding" evidence="15">
        <location>
            <begin position="76"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription factor DP C-terminal" evidence="14">
        <location>
            <begin position="166"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="270"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="15"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="38"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR009404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM01372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAI7726339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="327" mass="35751" checksum="EDD8B567B6046152" modified="2024-05-29" version="1">MGLNNNHLSRGRSWGTTASGQSVSTSGSVGSPSSRSEAAAHKLNHLDIHADDDAGSHGAVGSMKKKRAPRATGGDKNGRGLRQFSMKVCEKVESKGRTTYNEVADELVAEFGDPGNGVQASDQPQYDEKNIRRRVYDALNVLMALDIISKDKKEIHWKGLPQSSLTSIEELKKNRLALQSRFEKKSAYLRELQDQNLIQRNEQLYSSGNVPTGGVALPFILVQTRPHATVEVEISEDMQLVHFDFNSTPFEIHDDNHVLKAMKLCDQSKGGDDIPNNKPMDGDACSSPSPTLHPPSALTLQTTIPSTRPSTSPPLPGILKARVKHEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G3F6</accession>
    <name>A0AAD5G3F6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">K+ potassium transporter integral membrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_023989</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HAK/KUP transporter (TC 2.A.72.3) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011731">
        <property type="protein sequence ID" value="KAI7726193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G3F6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000325">
        <property type="term" value="C:plant-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015079">
        <property type="term" value="F:potassium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003855">
        <property type="entry name" value="K+_transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053951">
        <property type="entry name" value="K_trans_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30540">
        <property type="entry name" value="OSMOTIC STRESS POTASSIUM TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30540:SF8">
        <property type="entry name" value="POTASSIUM TRANSPORTER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02705">
        <property type="entry name" value="K_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="229"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="320"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="362"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="385"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="435"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="466"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="506"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="558"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="domain" description="K+ potassium transporter integral membrane" evidence="4">
        <location>
            <begin position="195"/>
            <end position="588"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7726193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="590" mass="65065" checksum="0890803BE3D67B62" modified="2024-05-29" version="1">MSRTQMSLIINKCIHHNIQNQRKKRSNKTKQQTVYYYSLTNLTLSHTPSLSRVSINHNKWIIIIINNNRIIIITESQYSNKQNLIKPMASSGSDLGSTSKGNGGLSSMDSIESHRWVFQDEEDDSEIDFEDNDAADGDDFNLHNDIDSEEEDSLYQKLIRTGPRIDSFGVEALEVPGAHRSDFEDASFGKSLFLVCQTLGVVFGDVGTSPLYTFSVMFSRAPIDGNEDIIGALSLVLYTLILIPLIKYILIVLLANDDGEGGTVALYSLICRHAKVSLLPNQLASDTRISSFRLKVPSAELERSLKIKEYLETSLALKKLVLVLVLAGTSMVIADGVVTPAMSVISAVRGFKVGVPTVEQDHVVMISVAYLVVLFSLQKYGTSKVGLVIGPALFIWFCSLGGIGIYNLVKYDSSVVKAFNPIHIYYYFKRNSYKAWYSLGGCLLCATGSEAMFADLCYFPVRTIQFTFVLLVLPCLMLGYMGQAAYLLNNGGDATQAFFSSIPDGAFWPVFLIANIAALIASRTMTTATFSCIKQSTALGCFPRLKIVHTSRKFMGQIYIPAINWFLMASAVLLVCTIASTNEIGNAYDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G3G9</accession>
    <name>A0AAD5G3G9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_019519</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011529">
        <property type="protein sequence ID" value="KAI7726822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G3G9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053197">
        <property type="entry name" value="F-box_SCFL_complex_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34223:SF101">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34223">
        <property type="entry name" value="OS11G0201299 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="235"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7726822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="418" mass="47420" checksum="51A7A57DC0B6C946" modified="2024-05-29" version="1">MDPSHGKRRKNEESDRLSSLSDDLIHKILSFTSIKLAIQMSVLSPRWSLCCDERIFSTFESLKNLTLKDCGTKGSKVFNVVAPQLKNLSIKDAKLRDFQCLISAPDLESLIYDGYYCLQLSAKDLCSLEKADIRIFRPKDAHQVLCLLQRLHNVKSLSLNLEIVELLSSYVELMSNLPSPFANLKSLKIYPQLTKLDECHTVKLSAEVKRYLLETSPGATFSMVTREDVRNMYNTELAQNLIRKLRASLEQEKARTATKIAKMRELGKSRYYVDSYIDLRWKYMSLQVKKVEEKGSDIISKLKEIKELLTELSASNRATIQPSFSTLCAEADTVTSMITDCMKMDHDENQSKWVKYSQQMGQMQLNICSKWVKCKWVTMSGALVDYNLQAAADPAVFCSSCCFNNGRTSRSQAVAAAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G545</accession>
    <name>A0AAD5G545_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Sugar phosphate transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_010190</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011301">
        <property type="protein sequence ID" value="KAI7727901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G545"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004853">
        <property type="entry name" value="Sugar_P_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050186">
        <property type="entry name" value="TPT_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11132">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03151">
        <property type="entry name" value="TPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="45"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="74"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="155"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="193"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="Sugar phosphate transporter" evidence="7">
        <location>
            <begin position="22"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="223"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7727901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="268" mass="29073" checksum="677D357164E672AC" modified="2024-05-29" version="1">MGEGQGFQLGTVGALSLSVISSVSIVICNKALISTLGFTFATTLTSWHLLVTFCSLHVALWMKFFEHKPFDSRAVMGFGVLNGISIGLLNLSLGFNSVGFYQMTKLAIIPCTVLLETLFFSKQFSKKVKFALTILLLGVGIATVTDLQLNLMGSVLSLFAIITTCVAQIVLGHLKTCLVLAFGYVLLHDPFSWRNILGILIAVVGMVLYSYFCTRDAQPKPVETSTQFSQSKESENDPLISVENGLNVSNEGSPPKPPVWSSDKDLHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G607</accession>
    <name>A0AAD5G607_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glyceraldehyde-3-phosphate dehydrogenase (phosphorylating)</fullName>
            <ecNumber evidence="3">1.2.1.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_029885</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.12" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010010862">
        <property type="protein sequence ID" value="KAI7729999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G607"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004365">
        <property type="term" value="F:glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18126">
        <property type="entry name" value="GAPDH_I_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000001">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020831">
        <property type="entry name" value="GlycerAld/Erythrose_P_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020830">
        <property type="entry name" value="GlycerAld_3-P_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020829">
        <property type="entry name" value="GlycerAld_3-P_DH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020828">
        <property type="entry name" value="GlycerAld_3-P_DH_NAD(P)-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10836">
        <property type="entry name" value="GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10836:SF112">
        <property type="entry name" value="GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE GAPC1, CYTOSOLIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02800">
        <property type="entry name" value="Gp_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00078">
        <property type="entry name" value="G3PDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00846">
        <property type="entry name" value="Gp_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00071">
        <property type="entry name" value="GAPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyceraldehyde 3-phosphate dehydrogenase NAD(P) binding" evidence="7">
        <location>
            <begin position="3"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7729999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="233" mass="24772" checksum="BB63ACECF3B0B4BD" modified="2024-05-29" version="1" fragment="single">FFLKSLAAPFIDDSARVTQGGAKKVVISAPSDNAPMFIVGVNEQEYTPDLNIVSNASCTTNCLAPLAKVINDRFGIVEGLMTTVHAMTATQKTVDGPSMKDWRGGRAASFNIIPSSTGAAKAVGKVLPALNGKLTGMAFRVPTVDVSKKATYEQVKAAIKEESKGKLKGILGYVDEDVVSTDFVGDSRSSIFDAKAGIALNDNFLKLVSWYDNECGYSSRVIDLICHIASVQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G641</accession>
    <name>A0AAD5G641_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Exostosin GT47 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_016442</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 47 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011119">
        <property type="protein sequence ID" value="KAI7728921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G641"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004263">
        <property type="entry name" value="Exostosin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040911">
        <property type="entry name" value="Exostosin_GT47"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11062">
        <property type="entry name" value="EXOSTOSIN HEPARAN SULFATE GLYCOSYLTRANSFERASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11062:SF337">
        <property type="entry name" value="OS04G0109900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03016">
        <property type="entry name" value="Exostosin_GT47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <feature type="domain" description="Exostosin GT47" evidence="6">
        <location>
            <begin position="8"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7728921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="317" mass="36957" checksum="477905F07EFC36C6" modified="2024-05-29" version="1">MVHDGPCKDIYTIEGRFIQEMEHNHHFRTKDAHKAHVYFMPFSVTWMVRYLYKPNTYDITPLQHFVSDYVRTISTKHSFWNTTHAADHFMLSCHDWAPHTSKGNLNLYHSSIRVLCNANSSEGFDPQKDVSLPEINLRHGNMPPNLLSPPPPESPRPHLAFFAGGVHGPIRPILLKYWKGQDPDLQVYEYLPKGLDYYSFMLASKYCLCPSGYEVASPRIVEAVYTECVPVIISDHYVLPFSDVLRWEAFSIQVKVSEIGELKQILTSVPENKYLRLKENLRAVRRHFVLNHPAERFDVYHMILHSVWLRRLNLKVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G8T0</accession>
    <name>A0AAD5G8T0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_026897</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010450">
        <property type="protein sequence ID" value="KAI7731473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G8T0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042185943" description="Secreted protein" evidence="1">
        <location>
            <begin position="25"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7731473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="109" mass="12359" checksum="49216D860C7AF052" modified="2024-05-29" version="1" fragment="single">CDSTLWLVLLWPLVRLATFALVDGSPEHKSNLFRKSPVPYRHVAHVQKTVLARQFSMEASRFSQHVHAPQQIVFGSPKCYKGRAKSGKALARDTTFCTDIFGSLETTWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GAG1</accession>
    <name>A0AAD5GAG1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_012192</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009766">
        <property type="protein sequence ID" value="KAI7734289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GAG1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7734289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="89" mass="9924" checksum="1BA9C32A4BE7F7F7" modified="2024-05-29" version="1">MGGGAKSHRMLKDWAMNCVDDKEIRNIEALYDDNVLKSLEIPSLPNLWCAYSVAKCYRMVAATNFLVRGINVLAELDALAHGQPLNPYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GB87</accession>
    <name>A0AAD5GB87_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">1-acylglycerol-3-phosphate O-acyltransferase</fullName>
            <ecNumber evidence="5">2.3.1.51</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_011699</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 1-acyl-sn-glycero-3-phosphate + an acyl-CoA = a 1,2-diacyl-sn-glycero-3-phosphate + CoA</text>
            <dbReference type="Rhea" id="RHEA:19709"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57970"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="EC" id="2.3.1.51"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.51" evidence="5"/>
    <dbReference type="EMBL" id="JAMZMK010009900">
        <property type="protein sequence ID" value="KAI7733828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GB87"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003841">
        <property type="term" value="F:1-acylglycerol-3-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07990">
        <property type="entry name" value="LPLAT_LCLAT1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032098">
        <property type="entry name" value="Acyltransf_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10983">
        <property type="entry name" value="1-ACYLGLYCEROL-3-PHOSPHATE ACYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10983:SF67">
        <property type="entry name" value="1-ACYLGLYCEROL-3-PHOSPHATE O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16076">
        <property type="entry name" value="Acyltransf_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="15"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="293"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="323"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="9">
        <location>
            <begin position="74"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7733828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="369" mass="41567" checksum="767754886E1D9BF9" modified="2024-05-29" version="1" fragment="single">SHTHTHTQTQRVRVMAIAAAAVIVPIGVLFFVSGLIVNLIQAIIFVTVRPFSKSLFRRINRQVAELLWLELVWIVDWWAGVKVNLYSDPETLNMMGCLGSTLAVMKKSSKFLPVIGWSMWFSEYLFLERSWAKDETTLKSGLRRLRDYPQPFWLALFVEGTRFTKAKLLAAQEYASSAGLPVPRNVLIPRTKGFVTSVSEMRSFAPAIYDMTVAIPKDSTPPTMLRLFKGQSSVVHVKVKRHLMKDLPETDEGVAQWCKDIFVAKDDLLDKHKELNAFPDSELYDIGRPLKSLVVVVSWACLLVLGIYKFLQWSNLLSSWKGLTITAIVLGIVTILMQILIQFSQSERSTPAKVAPTTSSNGSVQEKLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GCH0</accession>
    <name>A0AAD5GCH0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phytocyanin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_012302</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009090">
        <property type="protein sequence ID" value="KAI7737202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GCH0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039391">
        <property type="entry name" value="Phytocyanin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003245">
        <property type="entry name" value="Phytocyanin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33021">
        <property type="entry name" value="BLUE COPPER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33021:SF9">
        <property type="entry name" value="PUTATIVE, EXPRESSED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02298">
        <property type="entry name" value="Cu_bind_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51485">
        <property type="entry name" value="PHYTOCYANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042186791" description="Phytocyanin domain-containing protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Phytocyanin" evidence="2">
        <location>
            <begin position="27"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7737202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="127" mass="13632" checksum="46ED75AAB18B7DBB" modified="2024-05-29" version="1">MGKGKGGAVMAIVVICQLAAALQCAADAHVVGDSAGWNMNISGWGKNVRFLAGDTILFRYPKGKHNVVVLDMMACGRCYTNHGDKVYTSGDDIFTLKLGPTCFICSFPGHCPNMSLQIFATKQTQKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GEC8</accession>
    <name>A0AAD5GEC8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Aconitate hydratase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_008417</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the aconitase/IPM isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008822">
        <property type="protein sequence ID" value="KAI7738354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GEC8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016836">
        <property type="term" value="F:hydro-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043436">
        <property type="term" value="P:oxoacid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.499.10">
        <property type="entry name" value="Aconitase, domain 3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.19.10">
        <property type="entry name" value="Aconitase, domain 4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015931">
        <property type="entry name" value="Acnase/IPM_dHydase_lsu_aba_1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001030">
        <property type="entry name" value="Acoase/IPM_deHydtase_lsu_aba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015928">
        <property type="entry name" value="Aconitase/3IPM_dehydase_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006249">
        <property type="entry name" value="Aconitase/IRP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018136">
        <property type="entry name" value="Aconitase_4Fe-4S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036008">
        <property type="entry name" value="Aconitase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000573">
        <property type="entry name" value="AconitaseA/IPMdHydase_ssu_swvl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11670">
        <property type="entry name" value="ACONITASE/IRON-RESPONSIVE ELEMENT FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00330">
        <property type="entry name" value="Aconitase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00694">
        <property type="entry name" value="Aconitase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00415">
        <property type="entry name" value="ACONITASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53732">
        <property type="entry name" value="Aconitase iron-sulfur domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52016">
        <property type="entry name" value="LeuD/IlvD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01244">
        <property type="entry name" value="ACONITASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aconitase/3-isopropylmalate dehydratase large subunit alpha/beta/alpha" evidence="7">
        <location>
            <begin position="148"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="Aconitase A/isopropylmalate dehydratase small subunit swivel" evidence="8">
        <location>
            <begin position="682"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="89"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7738354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="726" mass="79586" checksum="E43C5036F8184110" modified="2024-05-29" version="1" fragment="single">TQNPFTRILKTLEKPGDGEYGKYYSLPSLNDSRIDRLPFSIRILLESAIRNCDEFQVTVKDVDKILDWQNTSPKKVEIPFKPARVLLQDATPGNEGSSSDGESEGGHDEEAAVTPIRQGSSRVKKQPAWMKDYHTNFAEEDMMALYSALDDHDFTGVPTIVDLAGMRNAMHKLGGDPSKINPLVPVDLVIDHSVQVDVSRSENALQANIEIELERNKERYSFLKWGAIAFDNMLVVPPELGLFTRVIFNTDDMLYSDTVFGTDSHTSMINGLGVAGWGVGGIEAEAAMLGQAMSMVLPGVVGFELSGSLKDGVTATDLVLTVAKMLRQHGVVGNFVEFYGKGMSEISLPDRANIANMSPEYGATMGFFPVDHVTLQYLKMTGRSDNTVKPWIKTSLGPGSRVVTKYLEKSGLLRYLQQLGFHIVGYGCTTCIGNSGDLDEPVASAIADNDIVAVAVLSGKRNFEGRIHPLTRANYLASPPIVVAYALAGTVDIDFETEPIGLGKNKMKIFLRDIWPSSEEVAEIVQSNLLPGMFKSVYETITEGHPTWNELHLPSSRLYVWDPNSTYIHEPPNLKDISMSPPGPQSVNDAYYLVNLGDSITTDHISPGGNIHKDSPAAKYLMELGVDRKDFNSYGSRRGNDEVMVRGTFANIRLVNKLLEGEVGPKTIHTPSGEKLSVYDAAMGVKAVIAKSFERIHRSNLVGMGIIPLCFQPGEDADTLELTGYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GEJ6</accession>
    <name>A0AAD5GEJ6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_010763</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008628">
        <property type="protein sequence ID" value="KAI7739420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GEJ6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7739420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="77" mass="8349" checksum="6FE295FF762BA14B" modified="2024-05-29" version="1" fragment="single">KKCYIVVPQEFDRRVKDLKLGPVDATGVVDAFVAVVLSQLLADVSLLKVIKSASNKATKSISGIYFRIHVLFSAPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GEV4</accession>
    <name>A0AAD5GEV4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_021593</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008951">
        <property type="protein sequence ID" value="KAI7737816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GEV4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009733">
        <property type="term" value="P:response to auxin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015495">
        <property type="entry name" value="Myb_TF_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10641">
        <property type="entry name" value="MYB FAMILY TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10641:SF1356">
        <property type="entry name" value="OS07G0484700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH myb-type" evidence="6">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="5">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="6">
        <location>
            <begin position="36"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="67"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="67"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7737816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="275" mass="31073" checksum="2D935DB803C787B0" modified="2024-05-29" version="1" fragment="single">HGPGNWRSIPTNTGLLRCSKSCRLRWTNYLRPGIKRAIASYLPQRTDNDIKNYWNTHLKKKLNKIQNNSSDEGYSNSTSRGLNSTLSSETATSKGKWERRLQTDIHMAKQALCEALSLDNKSINLSESSSSTNSQSQILQQKSMLPPPPHINHTTSAYTSSAENIARLLPNWIKKPKKSLYKADYSFREQFPSPSNEGLEYSLMFGFNNSNYSSNSEVSQSFSTETTSLLQDDTKLNIENAQLPSIGYLENWLLDDATGQETKDIFMNLSLEETD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GGH0</accession>
    <name>A0AAD5GGH0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Aminotransferase class I/classII large domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_032139</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 4 5">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008160">
        <property type="protein sequence ID" value="KAI7741597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GGH0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004838">
        <property type="term" value="F:L-tyrosine-2-oxoglutarate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006572">
        <property type="term" value="P:L-tyrosine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000048">
        <property type="entry name" value="tyrosine aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000040">
        <property type="entry name" value="Tyrosine aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005958">
        <property type="entry name" value="TyrNic_aminoTrfase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01265">
        <property type="entry name" value="tyr_nico_aTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45744">
        <property type="entry name" value="TYROSINE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45744:SF11">
        <property type="entry name" value="TYROSINE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000517">
        <property type="entry name" value="Tyr_transaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="6">
        <location>
            <begin position="47"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="5">
        <location>
            <position position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR000517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000517-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7741597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="423" mass="46861" checksum="0A66F5F22EDD5117" modified="2024-05-29" version="1">MEMENGSSPAKSWGFRANPELIMSSDNTVRGVLYMIRSKLNQLDTRPIIPLGHGDPSAFPCFRTTQVAEDAIVESLRSAKFNGYSPTDGILPARRAVAEYLSKDLPYELSPDDVFLTLGCVQAIQTIITVLTNPKANILLPRPGFPHYEAFAKACHLEVRHFDLLPDKDWEVDLDSVNTLADENTVAMVIINPGNPCGNVFTYQHLQKVAETAKKLGILVISDEVYDHLAFGKNPFVPMGTFGSITPVITLGSISKRWIVPGWRLGWLVINDPNGILKQHGIIDCITAYLNISADAPTFIQGAIPDILAKTKDDFFLKIVNIIKDCANICYEGIQDVPGIICPTRPEGSMFVMVKLDLSIFVDIKDDVDFCFKLAREESVIILPGMSVGLNNWLRVTFAIEPSALEEGIKRLKAFCERHSKKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GGY9</accession>
    <name>A0AAD5GGY9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">cyclin-dependent kinase</fullName>
            <ecNumber evidence="2">2.7.11.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_033046</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.22" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010008323">
        <property type="protein sequence ID" value="KAI7740919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GGY9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030332">
        <property type="term" value="F:cyclin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010389">
        <property type="term" value="P:regulation of G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000281">
        <property type="entry name" value="Cyclin-dependent kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000124">
        <property type="entry name" value="Cyclin-dependent kinase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF178">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE B2-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 11 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="1"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7740919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="247" mass="28272" checksum="5C17248F18D5F299" modified="2024-05-29" version="1">MESTKTAMEAFEKLEKVGEGTYGKVYKAREKATGKIVALKKTKLHEDEEGVPPTTLREISILRMLSRDPHVSLMYQLCKGVAFCHSHGILHRDLKPHNLLMDRKTLMLKIADLGLARAYKVPLPKYTHEILTLWYRAPEVLLGSTHYSTAVDMWSVGCIFAELVTNAAIFAGDSELQQLLHIFRLLGTPNEEIWPGVSKLKDWHEYPQWKPKPVSTAKMFEYEPSKRISAKKAMEHPYFDDLDKNHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GI35</accession>
    <name>A0AAD5GI35_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_029722</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008284">
        <property type="protein sequence ID" value="KAI7741026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GI35"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00018">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.730.10:FF:000002">
        <property type="entry name" value="AP2-like ethylene-responsive transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.730.10:FF:000003">
        <property type="entry name" value="AP2-like ethylene-responsive transcription factor ANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.730.10">
        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001471">
        <property type="entry name" value="AP2/ERF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32467">
        <property type="entry name" value="AP2-LIKE ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32467:SF157">
        <property type="entry name" value="AP2-LIKE ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR CRL5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00847">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00367">
        <property type="entry name" value="ETHRSPELEMNT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00380">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51032">
        <property type="entry name" value="AP2_ERF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="8">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="AP2/ERF" evidence="8">
        <location>
            <begin position="94"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="224"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="239"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="252"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7741026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="288" mass="32589" checksum="D1FE4802C1AF360F" modified="2024-05-29" version="1" fragment="single">WTGRYEAHLWDNSCKKEGQSRKGRQVYLGGYDMEEKAARAYDLAALKYWGPSTHINFPLENYEQEIVEMKNMSRQEYVAHLRRRSSGFSRGASIYRGVTRHHQHGRWQARIGRVAGNKDLYLGTFSTQEEAAEAYDVAAIKFRGVNAVTNFDISRYNVKKIIESNTLLAGELARRTKDVDTTNEILSDQPTAETGEAKTMLTEYNTNRNMLDWKMSLYDQNQTGPGVDVNESSKLGAHVSNDSSLVTSLSSSREDSPERNSSRETWIPTAEAAQMRAQIPVFAAWTDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GI91</accession>
    <name>A0AAD5GI91_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Casein kinase 1-like protein 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_000806</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007713">
        <property type="protein sequence ID" value="KAI7743607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GI91"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="67"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="108"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="136"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7743607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="220" mass="24639" checksum="08BA48B7E51888B4" modified="2024-05-29" version="1" fragment="single">VLCKSYPSEFISYFHYCRSLRFEDKPDYSYLKRLFRDLFIREGYQFDYVFDWTILKYPQIGATSRGRLQGGTTGINAGTSAEKPTRTSAGQDIRDRISGAVEAFSRRNLSGGARQGSRNKVADDVPSSKDVLPSSERIRSSRDGDSSKWAAIASSRPGSSVEPTEARLARHVSASGARISTTHRVQPHPSYETKPLGFSRTSKSGHEDQLRSFEFLSIRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GIQ9</accession>
    <name>A0AAD5GIQ9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_021611</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008223">
        <property type="protein sequence ID" value="KAI7741283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GIQ9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22160">
        <property type="entry name" value="F-box_AtFBL13-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053772">
        <property type="entry name" value="At1g61320/At1g61330-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053781">
        <property type="entry name" value="F-box_AtFBL13-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055357">
        <property type="entry name" value="LRR_At1g61320_AtMIF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34145:SF68">
        <property type="entry name" value="FBD DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34145">
        <property type="entry name" value="OS02G0105600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23622">
        <property type="entry name" value="LRR_At1g61320_AtMIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="21"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7741283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="491" mass="55946" checksum="B35D3A060FE7781F" modified="2024-05-29" version="1">MTNTRRRSAQSKVDNGRLNLTDFISKMPDDILVMILSLMPIKYAVVTSRLSTRWRHLWRNLIRLNFDGTEFFDKMADDDIFSDLERFKFIKQVSNVINSYNHPRVCDFRIRFDLDGCDTQQINEWIQFALDKKVERLELDLLDKNYDIRDPDESFEFRLPLAYDMGGQMLSLKKLFLTGVNLNGPNLFMILTNSPHLEKLYMFGVHVFPCVYVGGQGINLKYFKLVSCSGFESITLYGFDLVSFIYYGPEIELHLTDLPKLRELDVGEVSVGLEKNVFSQISSCALYLQALALDMRSTKEGLDVNAILKFPNVKKLKLGMSAEEDDCLLQFTSIAQACPILENFSICLHWFSPMKRRRKVRHVTPHVHKHLKFLEIIGYYGRISDLELAVYVIDNAAALKRIVIDPSCHAIGGDLSIQDCLKKQPAARSSAKRQLTPLLPPDAIFQKSIFIDIRIPTDYNTGAPHSSTANALVTPVARPPVFGTNPEVFGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GJJ4</accession>
    <name>A0AAD5GJJ4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_011320</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007733">
        <property type="protein sequence ID" value="KAI7743469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GJJ4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057235">
        <property type="entry name" value="DUF7913"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057237">
        <property type="entry name" value="DUF7915"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33913">
        <property type="entry name" value="ALEURONE LAYER MORPHOGENESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33913:SF1">
        <property type="entry name" value="DRBM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25500">
        <property type="entry name" value="DUF7913"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25502">
        <property type="entry name" value="DUF7915"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7913" evidence="2">
        <location>
            <begin position="14"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7915" evidence="3">
        <location>
            <begin position="171"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="291"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="608"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="671"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="309"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="363"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="397"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="414"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="431"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="479"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="497"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="530"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="613"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="628"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="684"/>
            <end position="693"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7743469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="693" mass="77355" checksum="54FE3100F8C9A0CC" modified="2024-05-29" version="1">MGFKGAMDIDFPTEVGPTEETVDALMEYLVGPLLPLKHTDFTKETPSESKQKSVAKQVHAAVVLYNYYHLNHNQESQFLKFDQFCKLATMLKPGITHHMKYTCQSDNTVLDDPENQLSITEKAIMDACTVSETLRNASAKISNIIKEWKVTKVAVLLIDSKKENCFLQFNNGVWSVIEKEVYPENKKRKKIMVEYDKEDLQKLAFLAVKEVAVYSLSQAKTATHFYIVQSTQKINEDNLVPIQDVISSLQGPVVKKCLSSWVITPVVEYYYLLPYAEILSKLFSRESNNLPHQIKDGSADVSINQGSQKSREKEKSNDSKDSNSTLKSNQRSTKESDQSKEVSTNLQKRSKLDSELVHISNCVKEEPTDSKDSKSTYKSNQCREKDSGESTEVSDSLPKKSKLDSECVHISNSVKEEPCDSKDSTSTYKYDQCEKDSGESKEASDNLGKKSKLDSEFVHIAIKEEPHDSKGTHTSNQCSEKENGQSKEVSDNLQETSKLDSETVHVSNSDKEEPHDGKASKNTYNSNQSSEKESGESEKVSDILQKKSKLESDVVHISSSSKEESHDSQVSKNGYESNQFGYTVEPVGKVADDAITSQFAARSVDQKVKESENSIGNSITVSNGSGVKQEDINGSCKDELNDSSSGASNKLENDKLLVCFQKENQNTKVQAPLKVYRHVKRSTPTKSTNSKVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GJM1</accession>
    <name>A0AAD5GJM1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_011620</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007957">
        <property type="protein sequence ID" value="KAI7742586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GJM1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7742586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="81" mass="8936" checksum="310A69342D6F1CB5" modified="2024-05-29" version="1" fragment="single">DYDGADEAWSNVIGIYETRGSWQMPVVVMAKSKGARAKVDHWHTPAWVRHLRGRDTCVGPGRDKLLKKVVAAANGSMDSDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GLT3</accession>
    <name>A0AAD5GLT3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_024748</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006699">
        <property type="protein sequence ID" value="KAI7747732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GLT3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7747732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="46" mass="4842" checksum="158611BE7CB548FD" modified="2024-05-29" version="1" fragment="single">QATKNGTMTVNNATKAPSLSPASLEPKMTTLGGLSKTLMTKEACWM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GMV0</accession>
    <name>A0AAD5GMV0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">squalene monooxygenase</fullName>
            <ecNumber evidence="5">1.14.14.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">M8C21_026855</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">AA19_3_7</strain>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Component of the triterpene saponins (e.g. ginsenosides or panaxosides) and phytosterols biosynthetic pathways. Catalyzes the first oxygenation step in sterol biosynthesis and is suggested to be one of the rate-limiting enzymes in this pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>squalene + reduced [NADPH--hemoprotein reductase] + O2 = (S)-2,3-epoxysqualene + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25282"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15440"/>
            <dbReference type="ChEBI" id="CHEBI:15441"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="EC" id="1.14.14.17"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:25283"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Terpene metabolism; lanosterol biosynthesis; lanosterol from farnesyl diphosphate: step 2/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the squalene monooxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.14.17" evidence="5"/>
    <dbReference type="EMBL" id="JAMZMK010007065">
        <property type="protein sequence ID" value="KAI7745991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GMV0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004506">
        <property type="term" value="F:squalene monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009725">
        <property type="term" value="P:response to hormone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016126">
        <property type="term" value="P:sterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046246">
        <property type="term" value="P:terpene biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000074">
        <property type="entry name" value="Squalene monooxygenase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013698">
        <property type="entry name" value="Squalene_epoxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040125">
        <property type="entry name" value="Squalene_monox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10835">
        <property type="entry name" value="SQUALENE MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10835:SF0">
        <property type="entry name" value="SQUALENE MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08491">
        <property type="entry name" value="SE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0274">FAD</keyword>
    <keyword evidence="6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Squalene epoxidase" evidence="15">
        <location>
            <begin position="120"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="Squalene epoxidase" evidence="15">
        <location>
            <begin position="496"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="363"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="370"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="609"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00051556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00056623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF08491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAI7745991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="609" mass="67864" checksum="BE0486EC6636BBA4" modified="2024-05-29" version="1" fragment="single">DLTEPDRIVGELLQPGGYLKLVELGLEDCLDGIEAQQVVGYALYMDGRNIKLSYPLEKCEFDVSGRSFHNGRFIQRMREKAASLPNVKLEQGTVTSLLEEHGTIKGVCYKAKDGQVMTAHAPLTIVCDGCFSNLRRALCKPKVEVPSCFVGMVLKNVDLPYANHGHVLLVDPSPILFYPISNTEVRCLVDIPGQKLPSIANGEMTNYLKTFVAPQIPTKLYDAFVAEVDKGNIRTMPNRSMPANPQPTPGALLIGDAFNMRHPLTGGGMTVALSDIVLLRDLLRPLRNLNDAPTLCRVRQMFFPATVPAYYKAPRSYSKTRRKSKDIHRNRINNGFKLTDTDGAADVIIVGAVNLHDHNNWQHNKRISRQTKPTPNSLGNKKAKQTFYPKLKYAQRPSQPNRLDEGGVEEALFMSLNMIYIKALDLNLNRMDDECMDLTEPDRIVGELLQPGGYLKLVELGLEGKVQFLLNCLDGIEAQQKFSQWTIYSTDEEESCIASEVEVPSCFVGMVLKNVDLPYANHGHVMLVDPSPILFYPISNTEVRCLVDIPGQKLPSIANGEMTNYLKNFVAPQAAFGIILPIIKAEGVRQMFFPVTIPAYYKAPRVLKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GR30</accession>
    <name>A0AAD5GR30_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_019308</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006192">
        <property type="protein sequence ID" value="KAI7750174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GR30"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7750174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="72" mass="8406" checksum="58DA42B81B99C3AA" modified="2024-05-29" version="1" fragment="single">MILSLFAGLTVPGNFRSILPDSDENRVLLYVQSIGSFDARHTTRTVIHILCHIEANMHMYTQYDTKHEFYIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GSU1</accession>
    <name>A0AAD5GSU1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Poly [ADP-ribose] polymerase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_009592</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010004554">
        <property type="protein sequence ID" value="KAI7754077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GSU1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003950">
        <property type="term" value="F:NAD+ poly-ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.228.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012317">
        <property type="entry name" value="Poly(ADP-ribose)pol_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044964">
        <property type="entry name" value="RCD1/SRO1-5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022003">
        <property type="entry name" value="RST"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32263">
        <property type="entry name" value="INACTIVE POLY [ADP-RIBOSE] POLYMERASE SRO4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32263:SF12">
        <property type="entry name" value="INACTIVE POLY [ADP-RIBOSE] POLYMERASE SRO4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12174">
        <property type="entry name" value="RST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56399">
        <property type="entry name" value="ADP-ribosylation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51059">
        <property type="entry name" value="PARP_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51879">
        <property type="entry name" value="RST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="PARP catalytic" evidence="6">
        <location>
            <begin position="60"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="RST" evidence="7">
        <location>
            <begin position="289"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="39"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7754077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="364" mass="40116" checksum="5DE74221B50B0962" modified="2024-05-29" version="1">MIINNTVEEEEEVSSSFGTKGGVYKHTHVVIERSVSGDLSGNLSGDDSSSVSDCESGVSGTRVVEPDSIGITGLVRLDESDKLCGIIGKKFVSRLSEIGIDAVVDCIHRNMFNANVISQARISSFQIFVKALQKRNGGDANIKYAWFGGSKDEINNIIIHGFGHDNIMKNDGFNHGVVLSADHSPLDSVEAAIADEDGIKHILLCRVLLGKTEVVNRGSMQSHPSSDDYQSGVDDLVSPKKYIVWSSQMNTHILPEFVISFKTLTSVNSRSLQECDQGVKLDGVPVRVKKPVSPWMPIPELIAALSEFLPPKAIKEITQFRRNFIEHKIPRCDMIRGIRQITGDRLLLMVLKNFNEQRGRKRSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GUE6</accession>
    <name>A0AAD5GUE6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Leucine-rich repeat-containing N-terminal plant-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">M8C21_010357</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">AA19_3_7</strain>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RLP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000966">
        <property type="protein sequence ID" value="KAI7755635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GUE6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001678">
        <property type="entry name" value="Calmodulin-binding receptor kinase CaMRLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000413">
        <property type="entry name" value="Inactive leucine-rich repeat receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000275">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000041">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050647">
        <property type="entry name" value="Plant_LRR-RLKs"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48056">
        <property type="entry name" value="LRR RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48056:SF81">
        <property type="entry name" value="RECEPTOR PROTEIN-TYROSINE KINASE CEPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042090297" description="Leucine-rich repeat-containing N-terminal plant-type domain-containing protein" evidence="13">
        <location>
            <begin position="22"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="14">
        <location>
            <begin position="27"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="15">
        <location>
            <begin position="220"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAI7755635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="511" mass="55218" checksum="024B981C6F2EDA9E" modified="2024-05-29" version="1">MDVIRLCIIAFMSIASLTIKADESLNLNDDILGLIVFKASVSDPSSQLSSWNQDDETPCSWKFITCNPVSRRVTGISLDGLNLSGKIGRGLEKLPYLKVLSLANNNFTGFINPELSLLIHLKHLNLSQNSFSGRIPGSLLNPGSIQFLDLSRNSLSGPVPEELFRNCLLLRSLSLSGNNLEGPIPNSLSNCKTLNHLNLSNNQFSGNPVFESGIWTLIRIRTLDLSHNSFSGAIPNGVFALHDLKQLSLNGNQFSGSLPSDLGLCLHLKTLDLSNNLFIETVPQSFHALSSLSLLNLANNKLTGEFPQWIGSLTSLESLDFSGNGFTGILPESIGGLTSLKYLSLSANSLSGTIPMSLDSSTKLSVISLSGNKFNGSIPNGIFDLGFDQIDLSRNELTGSIPQGSSKLFESLQSLDLSSNRLTGDIPAEIGLNSKLRYLNLSWNNFETRMPPELGYFQNLTVLDLRNSAFLGSIPQELCDSGSLGILQLDGNSFSGSIPDEIGNCSSLYLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GVZ3</accession>
    <name>A0AAD5GVZ3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_007185</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein disulfide isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005276">
        <property type="protein sequence ID" value="KAI7753763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GVZ3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003756">
        <property type="term" value="F:protein disulfide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034976">
        <property type="term" value="P:response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02961">
        <property type="entry name" value="PDI_a_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000107">
        <property type="entry name" value="Protein disulfide-isomerase 5-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18929">
        <property type="entry name" value="PROTEIN DISULFIDE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18929:SF218">
        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE 5-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13848">
        <property type="entry name" value="Thioredoxin_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00421">
        <property type="entry name" value="THIOREDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042094159" description="Thioredoxin domain-containing protein" evidence="7">
        <location>
            <begin position="18"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="361"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="8">
        <location>
            <begin position="5"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="393"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="410"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7753763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="423" mass="47331" checksum="A86BE0E6E3DB9A51" modified="2024-05-29" version="1">MLLLLLLIVVCPMISSCHEFTVDKGKVLELDESNFDSAISSLDFVFVDFYAPWCGHCKRLSPELDKAAPILSGLKTPVVIAKIDADKYSHIASKYKIDGFPTLKLFMHGVPTDYNGPRKSDLLVRYLRKFVAPDVTVLKSDSGITEFVEAAGTFFPIFIGFGLDESAISNLAIKFKKNAWFSVAKDFSENMMTLYDFDKAPALLALHPNFNEQSIFYGPFEDKFLEEFIQQSLLPLTLPISGDNLKLLKDDKRKIVLTIVEDETHYESKGLIKLLRAAASANRDFVFAYVGYNQWKDFAEAFEVGRKTPLPKMVVWDGNEVFFAVIDSESIQGEDQGSQITLFLERYREGKVIEKSMGPSFVGYISSLLGMRTVYILVFLAAVMFLIATAGKEEPSDVGPRDPASTSVADNRETTRLSADKED</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GWE3</accession>
    <name>A0AAD5GWE3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_007666</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000040">
        <property type="protein sequence ID" value="KAI7757930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GWE3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051693">
        <property type="term" value="P:actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11293">
        <property type="entry name" value="gelsolin_S4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.950.10">
        <property type="entry name" value="Villin headpiece domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007123">
        <property type="entry name" value="Gelsolin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007122">
        <property type="entry name" value="Villin/Gelsolin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003128">
        <property type="entry name" value="Villin_headpiece"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036886">
        <property type="entry name" value="Villin_headpiece_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11977">
        <property type="entry name" value="VILLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11977:SF25">
        <property type="entry name" value="VILLIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00626">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02209">
        <property type="entry name" value="VHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00597">
        <property type="entry name" value="GELSOLIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00262">
        <property type="entry name" value="GEL"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00153">
        <property type="entry name" value="VHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47050">
        <property type="entry name" value="VHP, Villin headpiece domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51089">
        <property type="entry name" value="HP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0117">Actin capping</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HP" evidence="3">
        <location>
            <begin position="553"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="431"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="521"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="434"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="521"/>
            <end position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7757930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="618" mass="69822" checksum="AB2887007697416E" modified="2024-05-29" version="1">MLESNKCYMLDGDIQIFVWMGSITSITERKTSISAAEGFLRAQEKPVNTQLAILKEGSETGIFRSYFDDWPLTVEPKLYEEGRGKVAAMFKQTGYDVDELPDEDEKPHIVCNGTLKVWRVNNGKLSPVPVVDQRKFYSGDCYTVQYIYSANGREERLFYIWLGNKSSPEDRADAISLTNVIVSSNKAEPVLARIVENKEPSQFFMIFQTLIIYKGGMGSGYKSFIAEKGLDDETYDDKKTALFRIQGTNRDNMQAVQVDQPAWQPILIREGSEPDVFWDALGGKTEYPKEREIKRSIEDPHLFVCIFSEGVASQSSDLKVKEIFSYTQDELTTEDVLILDCYTEIYVWVGHNSVVKSKQHALSIGLAFLEKDILGECLSTDTSTYVVIEGHEPPFFTRFFDWDASKANMLGNSFERKLAILKGQTQKLEAPLRNSPTIYSKETTPNGLRRPSPTPNGLRRPSPTPNGSIRLIPASNGLTRRQSLDSYTITSTSPTFSGLDFNPANNRRFSSPPVPIMIPSASSPDVHSADNTHVSASSTAEVEILPSKDSGPKKELINYPYDRVKVNSKDPVHDIDITKREAYLSDEEFLEKFAMSKQVFYQLPRWKQNKLKMSLFLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GYQ5</accession>
    <name>A0AAD5GYQ5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">alpha,alpha-trehalose-phosphate synthase (UDP-forming)</fullName>
            <ecNumber evidence="3">2.4.1.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_003378</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>D-glucose 6-phosphate + UDP-alpha-D-glucose = alpha,alpha-trehalose 6-phosphate + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18889"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58429"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="ChEBI" id="CHEBI:61548"/>
            <dbReference type="EC" id="2.4.1.15"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the C-terminal section; belongs to the trehalose phosphatase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">In the N-terminal section; belongs to the glycosyltransferase 20 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.15" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010000195">
        <property type="protein sequence ID" value="KAI7757001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003825">
        <property type="term" value="F:alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004805">
        <property type="term" value="F:trehalose-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005992">
        <property type="term" value="P:trehalose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03788">
        <property type="entry name" value="GT20_TPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01627">
        <property type="entry name" value="HAD_TPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000010">
        <property type="entry name" value="Alpha,alpha-trehalose-phosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000052">
        <property type="entry name" value="Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000054">
        <property type="entry name" value="alpha,alpha-trehalose-phosphate synthase [UDP-forming] 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000017">
        <property type="entry name" value="alpha,alpha-trehalose-phosphate synthase [UDP-forming] 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001830">
        <property type="entry name" value="Glyco_trans_20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006379">
        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003337">
        <property type="entry name" value="Trehalose_PPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01484">
        <property type="entry name" value="HAD-SF-IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00685">
        <property type="entry name" value="T6PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788:SF104">
        <property type="entry name" value="GLYCOSYL TRANSFERASE, FAMILY 20-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788">
        <property type="entry name" value="TREHALOSE-6-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00982">
        <property type="entry name" value="Glyco_transf_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02358">
        <property type="entry name" value="Trehalose_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7757001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="849" mass="96558" checksum="A398F630C0213F98" modified="2024-05-29" version="1">MASKSYANILDLEPDKLLDIPQTPKSLPRVLTMPGIIDDVDDSNVDSEKVPSGCRERKIIVANMLPLHIQRDPDTLKLXXXXDEDSLLWQLKDGLDPETEVVYIGSLKVEVDACEQEEVAQKLLEEYNCVPTFLPHDLYKKYYGGFCKHQLWPLFHYMLPMSADHADRFDRALWKAYVSANKIFADKVMEVANPEEDYIWVHDYHLMILPTFLRNRCNRVKLGFFLHSPFPSSEIYRTLPVRDEILKGLLNCDLIGFHTFDYARHFLSCCSRMMGLDYESKRGHIGLDYLGRTVYIKILPVGIHMGRLESVLNLPITKRKVKEIAERFKGKKLIVGVDDMDIFKGLSLKLLAFEYLLRSYPLLRGKLVLIQIVNPARSAGKDVQEAKRETYLILNRINEAYGSPDYQPVVIIDRPVARYEKSAYYSMADCCIVNAVRDGMNLVPYKYIVCRQHSPHFDEKSARTSMLVVSEFVGCSPSLSGAIRVNPWDIESVAVAINSAVVMKEPEKQLRHEKHYRYVSSHDVAYWARSFMQDLERASKDHYNKRCWGMGFGLGFRVVSLSPEFRKLSADCIVSAYKRSNKRAIFLDYDGTLVPHSSIVKTPGPELINILNTLCDDPKNIVFVISGRGKDTLSEWLDSCERLGLAAEHGYFMRWNRNSDWQPGIVGADFEWKEIAKPVMELYKEATDGSTIEFKDSGLVWHHQDADPDFGSCQANELVLHLENVLANEPALVKRGRYIVEVKPQGVTKGLVADKVLSVMTEQGEPPDFITCIGDDRSDEDMFESIVTYAASNTSDIFACTVGRKPSKAKYYLDDTTEVVKLLSGLANTSHPKPSNNPPRFQVTFDAVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BKT0</accession>
    <name>A0AAD5BKT0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_010497</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012040">
        <property type="protein sequence ID" value="KAI7725147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BKT0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7725147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="54" mass="6123" checksum="0895649711903072" modified="2024-05-29" version="1">MKEVTNATKRTPAINNKKDPKTTPLFAAKQPLLFEEPDTGNSSHTEIKHPRKQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BL72</accession>
    <name>A0AAD5BL72_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">M8C21_017952</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="24">AA19_3_7</strain>
            <tissue evidence="24">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="EMBL" id="JAMZMK010011977">
        <property type="protein sequence ID" value="KAI7725311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BL72"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048827">
        <property type="term" value="P:phyllome development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090567">
        <property type="term" value="P:reproductive shoot system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010103">
        <property type="term" value="P:stomatal complex morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000290">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000288">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000261">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000077">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000219">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051716">
        <property type="entry name" value="Plant_RL_S/T_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48053">
        <property type="entry name" value="LEUCINE RICH REPEAT FAMILY PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48053:SF120">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="15 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-0675">Receptor</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 22" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="710"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="777"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="91"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="806"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="KAI7725311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="999" mass="110539" checksum="4EA19F2A673D7113" modified="2024-05-29" version="1">MNIDEYMEIKKQQTEIIPPNPNPKSVIQSLQHFHNLSPAMEYQYPTHTIQASKFHQQPPTQPINPTQRRHHHSFTTCHSAVTTTLSQPVTGRRFTGQKEKDSRPYIAPSPPPLHSAVTTTLTQRRHHHPRWWPAVAIHERGRGNDTVGGSRKVGVLHGWTTLYSGGTLLEIKKSFRDVNNVLYDWTGSPSSDYCMWRGVSCDNITFNVLALNLSGLNLDGELSPSIGELKGDLRGNRLSGQIPDEIGDCSNLQNLDLSFNMLTGDIPFSISKLKQLELLILKSNQLIGPIPSTLSQIPNLKILDLAQNQLSGEIPRLIYWNEVLQYLGLRSNNLVGELSPDICQLTGLCDVRNNSLSGTIPSNIGCVHCVRDLSYNRLTGEIPFNIGFLQVATLSLQGNQLSGEIPSVIGLMQALAVLDLSCNNLSGQIPPIIGNLTYTEKLYLHNNKLTGSIPPELGNMSKLHYLELNDNQLTGHIPPELGKLTDLFDLNVAGNKLEGHIPDNLSSCTNLNSLNVHGNKLKGTIPAQLQRLESMTYLNLSSNNLAGPIPIEISRIGNLDTLDLSNNKISGPIPSPLGDLEHLLKLNLSRNEFTGYLPAEFGNLRSVMDIDLSYNRLSGVLPQELGQLQNLFTLKLESNNFTGGLTSLVNCLSLSVLNVSYNNLIGDIPTGNNFSKFSPDSFLANPGLCSSSLSSCHASHSTERVTIPRAAILGIALGALILLLMILVAVCRPNNSKPFMDGSYDKPVHYSSPKLVILHMNLALHVYDDIMRMTENLSEKYIIGYGASSTVYKCVLKNCRPVAIKKLYTHYPEYLKEFETELETVGSIKHRNLVSLQGYSLSPSGNLLFYDYMENGSLWDLLHGPLKKKKLDWDTRIQIALGAAQGLAYLHHDCSPRIIHRDVKSSNILLDKDFEAHLTDFGIAKSLCTSKTFTSTYIMGTIGYIDPEYARTSRLTEKSDVYSYGVVLLELLTGRKAVDNESNLHHLVSLLALIVFLLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BM01</accession>
    <name>A0AAD5BM01_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Serine hydroxymethyltransferase</fullName>
            <ecNumber evidence="9">2.1.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_024772</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Interconversion of serine and glycine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + glycine + H2O = (6S)-5,6,7,8-tetrahydrofolate + L-serine</text>
            <dbReference type="Rhea" id="RHEA:15481"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="EC" id="2.1.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 8 9">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 9">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 9">Belongs to the SHMT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.1" evidence="9"/>
    <dbReference type="EMBL" id="JAMZMK010011844">
        <property type="protein sequence ID" value="KAI7725842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BM01"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004372">
        <property type="term" value="F:glycine hydroxymethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019264">
        <property type="term" value="P:glycine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00378">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000005">
        <property type="entry name" value="Serine hydroxymethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001085">
        <property type="entry name" value="Ser_HO-MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049943">
        <property type="entry name" value="Ser_HO-MeTrfase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019798">
        <property type="entry name" value="Ser_HO-MeTrfase_PLP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039429">
        <property type="entry name" value="SHMT-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680:SF35">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00464">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000412">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00096">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="7 8 9" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Serine hydroxymethyltransferase-like" evidence="10">
        <location>
            <begin position="2"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="Serine hydroxymethyltransferase-like" evidence="10">
        <location>
            <begin position="73"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="8">
        <location>
            <position position="184"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000412-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7725842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="411" mass="45560" checksum="CB36056CDD577D52" modified="2024-05-29" version="1" fragment="single">TADPEIFDLIEHEKRRQSRGIELIASENFTSYAVIQALGSPLTNKYSEGMPGNRYYGGNEYIDQIENLCRSRGHLTHGYYTSGGKKISATSIYFESLPYKVNSETGYIDYEKMEEKALDFRPKLIICGGSAYPRDWDYKRIRSIADKCGALMLCDMAHISGLVAAQEAADPFEYCDVVTTTTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDFEDKINFAVFPALQGGPHNHQIGALAVALKQAMSPGFKAYAKQVRANAVALGNYLMSKDYKLVTGGTENHLVLWDLRPLGLTGNKVEKLCDLANITVNKNAVFGDSSALAPGGVRIGTPAMTSRGLVEKDFEQIGEFLHRAIQITLSIQKEFGKLLKDFNKGLINNKDIEQLKADVEKFSGSFDMPGFSLADMKYKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BN57</accession>
    <name>A0AAD5BN57_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">glutathione transferase</fullName>
            <ecNumber evidence="2">2.5.1.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_031698</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GST superfamily. Theta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010011670">
        <property type="protein sequence ID" value="KAI7726436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BN57"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009407">
        <property type="term" value="P:toxin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03183">
        <property type="entry name" value="GST_C_Theta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03050">
        <property type="entry name" value="GST_N_Theta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000039">
        <property type="entry name" value="Glutathione S-transferase theta-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040077">
        <property type="entry name" value="GST_C_Theta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040075">
        <property type="entry name" value="GST_N_Theta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043377">
        <property type="entry name" value="GSTT1/2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44750">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE T1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44750:SF1">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE T1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14497">
        <property type="entry name" value="GST_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01153">
        <property type="entry name" value="Main.4:_Theta-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0216">Detoxification</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="6">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="7">
        <location>
            <begin position="89"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7726436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="237" mass="26935" checksum="CE67A2589FEED531" modified="2024-05-29" version="1">MTLKVYCDRMSQPSRAILIFCKMNGIDFEEVTIDILKSQQHTPEYKAVNPMCQVPTIVDGDFTLFESHAILIYLSCKYPGVASHWYPSDLIERAKVHSVLDWHHANLRRGSVGVIVNNVLLPLKGLPPNPQAAEEAEKTLEKALTILETFWLKDGPFLVGRSQPSIADLNLVSEIMELQLLSQELHDRLLSPYKKVLQWIEDTKSATAPHFEEIHGVLFKTRKEFHELMATKSGKTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BQ33</accession>
    <name>A0AAD5BQ33_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_003837</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011356">
        <property type="protein sequence ID" value="KAI7727533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BQ33"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7727533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="146" mass="15598" checksum="1914F6A298BCA99B" modified="2024-05-29" version="1" fragment="single">RNQTRLQHNLMYLAAIADCQPKQSPMRLQPYTNSMQQQMQMQSQHMVPQPHMMAQSHTGFGQQSPYSVHHHALQSQIGMVPSGTSGTSLDGEGSGLGQPVFGDGSAGGISGSTRWSMPKENISTGSTEGIAGTHPQKKGKLSLPGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BRE1</accession>
    <name>A0AAD5BRE1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_005859</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011303">
        <property type="protein sequence ID" value="KAI7727874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BRE1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47337">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT (TPR)-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47337:SF1">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT (TPR)-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144232">
        <property type="entry name" value="HIT/MYND zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7727874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="568" mass="63525" checksum="DD197E4CA432C263" modified="2024-05-29" version="1">MLMQALRVAPRDVDVMGKNLVATLYVNRAAVLQKMGLYLESLRDCNRALAICRSYSKAWYRRGKANTSMGNYNDAVCDLKVSLCMEHSLSGKRQIEAELKLILSQNNREDFPPNGIKCKESDIHDQLQDIQLQCVETETKGRGMVSLTDIPQATLLHAEEPYAAIVSKNCRETHCHFCFNELPADIVPCSSCSMALYCSQLCQIHARGQDQQFHTKNHDIGFDLSGDLENYVTSVTEAIDDGVNIKQNAEHKHECGVNWPAALPAEIVLAGRAIVKSIEQNRHFNESSSIVNLELCQNYLQLGTEAKLELHISSIVLMKCLHHCYRSDIPLNGDTLSQVGQWSCNKRRSSLKEQYAFVFLDSSVANYEQQKINPFSCVLTLQVGRFKHDEVKKVASFLCKETGSTHQIKPGFCLSCGAFRDLEASNAVVKKAEICIGRLEGLLDSGECHNTALSNALRSLDLLKSALHPYNKKVAEAEDYIAQAFCLIGELQRSRDHCQASIKILEKLYGDDHSVIGNEMVKLASIQLSLGDHAVEETRNRISLIFSQHYGSQVDAVFPHLQFLKRSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BRJ0</accession>
    <name>A0AAD5BRJ0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_003337</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011497">
        <property type="protein sequence ID" value="KAI7726889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BRJ0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7726889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="111" mass="12622" checksum="9A8230A70E0303C0" modified="2024-05-29" version="1">MMTVTPTVVWVQDELREAIRSGGRLLRFWGFCMKNTYILNHSLRLSVYDDETVCRRKLRQPIRFGKAVEFDAFRYGFSKGKKNCPVPVSRISITLVAAAAAASLHPIPMMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BT04</accession>
    <name>A0AAD5BT04_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_015313</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011114">
        <property type="protein sequence ID" value="KAI7728932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BT04"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006447">
        <property type="entry name" value="Myb_dom_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046955">
        <property type="entry name" value="PHR1-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01557">
        <property type="entry name" value="myb_SHAQKYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31499">
        <property type="entry name" value="MYB FAMILY TRANSCRIPTION FACTOR PHL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31499:SF11">
        <property type="entry name" value="MYB FAMILY TRANSCRIPTION FACTOR PHL8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7728932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="106" mass="12129" checksum="98061EC6FCA82FCB" modified="2024-05-29" version="1" fragment="single">QSFGKCLFCKVHRNELSRGCSFLGKSIDTLLILIIFNLSSTLQAPSNLIERTQHGYYDSSMQLLSLEARRVETTPKSLMRVMSIHGLTLYHLKSHLQKYTLGTRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BT49</accession>
    <name>A0AAD5BT49_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">ADP-ribosylation factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_023828</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">GTP-binding protein involved in protein trafficking; modulates vesicle budding and uncoating within the Golgi apparatus.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011132">
        <property type="protein sequence ID" value="KAI7728849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BT49"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016004">
        <property type="term" value="F:phospholipase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04150">
        <property type="entry name" value="Arf1_5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003500">
        <property type="entry name" value="ADP-ribosylation factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045872">
        <property type="entry name" value="Arf1-5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11711">
        <property type="entry name" value="ADP RIBOSYLATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8 13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 11 12" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="10 13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4 13" id="KW-0519">Myristate</keyword>
    <keyword evidence="5 11 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 13" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 13" id="KW-0813">Transport</keyword>
    <feature type="binding site" evidence="11">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="105"/>
            <end position="108"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU369003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7728849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="160" mass="18409" checksum="98E802BB7A94188F" modified="2024-05-29" version="1">MGLTFTKLFSRLFAKKEMRILMVGLDAAGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRQRHWYIQSTCATSGEGLYEGLDWLSNNIANKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BT51</accession>
    <name>A0AAD5BT51_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Disease resistance R13L4/SHOC-2-like LRR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_021500</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RLP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011092">
        <property type="protein sequence ID" value="KAI7729005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BT51"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000111">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47988">
        <property type="entry name" value="SOMATIC EMBRYOGENESIS RECEPTOR KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="10">
        <location>
            <begin position="13"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7729005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="115" mass="12145" checksum="55B4569800FF8BF1" modified="2024-05-29" version="1">MPGQNLSGTLSPAIENLTNLQSIMLQDNAISGSIPGAIGKLRKLQTLDLSGNKFSGDLPSSFGDLMNLNLLRLNNNSLTGSVPESLSELGSLTLVDLSYNNLSGLLPKISARTFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BUD6</accession>
    <name>A0AAD5BUD6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cytochrome P450 724B1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">(22S)-22-hydroxycampesterol synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">M8C21_006137</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">AA19_3_7</strain>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>campesterol + reduced [NADPH--hemoprotein reductase] + O2 = (22S)-22-hydroxycampesterol + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:69835"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:28623"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:72331"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:69836"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Hormone biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="9">Plant hormone biosynthesis; brassinosteroid biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="11">Steroid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011180">
        <property type="protein sequence ID" value="KAI7728633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BUD6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016132">
        <property type="term" value="P:brassinosteroid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010268">
        <property type="term" value="P:brassinosteroid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016125">
        <property type="term" value="P:sterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11043">
        <property type="entry name" value="CYP90-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000057">
        <property type="entry name" value="Cytochrome P450 724B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24286">
        <property type="entry name" value="CYTOCHROME P450 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24286:SF37">
        <property type="entry name" value="CYTOCHROME P450 724B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 15" id="KW-0349">Heme</keyword>
    <keyword evidence="7 14 15" id="KW-0408">Iron</keyword>
    <keyword evidence="8 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 14 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="14">
        <location>
            <position position="441"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00052777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00060577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00067336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00077474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAI7728633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="493" mass="56606" checksum="B9B3F6801D09957B" modified="2024-05-29" version="1">MGVFWMVIFVGFVGFIFGLTLNHFLPLLFIKNNKLGDLPRGSFGYLPLLGETLAFLNPHPSTTIGSFLQEHCSRYGKVFKSHLFFSPTIVSCDQELNYFILQNEDKLFECSYPKPIHGVLGKISMLVVVGDTHKRLRNAALSLVSTTKSKHDFLTYIEKTTLQILDSWKDKKQVIFCQEARKFTFNVIVKQVLGLTPEEPQTVEILEDFLTFMRGLISFPLYIPGTPYANAVKARIRISSSVKQIIKERRRNNIISNNNYNICENKNINSHKKESDFLEILLGVDTLSEDEKVSFVLDALLGGYETTSILMALVVHFVAKSRAAFEQLKVEHASIRSKKNKDESLNWEDYKMMTFTQNIINEALRYGNIVKFLHRKALKDIKFKDYLIPAGWKVLPILSTVHLDPSIHSSPLEFYPWRWETQRQDQTSKKFTPFGGGSRCCPGSELARVEVAFFLHHLVQNFRWSVEGDDQPIAYPYVEFQRGLVLNVEHFAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BUV2</accession>
    <name>A0AAD5BUV2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_020924</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010930">
        <property type="protein sequence ID" value="KAI7729719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BUV2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044798">
        <property type="entry name" value="EAF1A/B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46774">
        <property type="entry name" value="CHROMATIN MODIFICATION-RELATED PROTEIN EAF1 A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46774:SF10">
        <property type="entry name" value="HOMEODOMAIN-LIKE, HELICASE_SANT-ASSOCIATED DOMAIN, MYB-LIKE DOMAIN PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="91"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7729719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="123" mass="12859" checksum="45A7D49C9DC438DC" modified="2024-05-29" version="1" fragment="single">RMRVPRIQFSRQGRDKRQGRMRAGVDASSNKCEVQSSQITLLQLVLYSIHMPLDLCDAISPSESLAPVGYQGPHAGGLPGPNNGTQASICSGSSPAYSPPGSSAMGLGNHLASASTPLSPTLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BWH1</accession>
    <name>A0AAD5BWH1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_020393</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010928">
        <property type="protein sequence ID" value="KAI7729743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BWH1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7729743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="117" mass="13104" checksum="8E8733DBE81C2BED" modified="2024-05-29" version="1" fragment="single">VCSTLILTQTQNTMQQVSSNNPNPMNSNNNPAPFSLRASHRRAHFEVNYRLPDDLDLVSDTFDTSSRSFEEIGSEDDLLCYDSIRMMMKALRILVATKTSVACVVLTDSTKEESIGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BYG4</accession>
    <name>A0AAD5BYG4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_020879</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010655">
        <property type="protein sequence ID" value="KAI7730774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BYG4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00132">
        <property type="entry name" value="CRIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00159">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.555.10:FF:000046">
        <property type="entry name" value="Rho GTPase-activating protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.810.10">
        <property type="entry name" value="CRIB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000095">
        <property type="entry name" value="CRIB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036936">
        <property type="entry name" value="CRIB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044785">
        <property type="entry name" value="RopGAP1-5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23177">
        <property type="entry name" value="MKIAA1688 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23177:SF64">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00786">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00285">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50108">
        <property type="entry name" value="CRIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRIB" evidence="3">
        <location>
            <begin position="103"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="4">
        <location>
            <begin position="148"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="389"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="389"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="419"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="453"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7730774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="462" mass="51596" checksum="AE6033516AAC8164" modified="2024-05-29" version="1">MTEILQISIGNENDGVYQQSCLINNNNSPCCSLYSVEESERRKRREREEEEREREVSLLAVVVTVVRKSLVGSCNSVKLFQDFGCDSNNNNNKSNNKSSKMEIGVPTDVRHVAHVTFDRFNGFLGLPVEFEPDVPRRPPSASTSVFGVSTESMQLSFDSRGNVVPTILLMMQSRLYAQGGLQAEGIFRINGDNGQEEQIREQLNRGEVPENIDVHSLAGLIKAWFRELPNGVLDSIEPEQVMQAQSEEECARLVSLLPPTEASLMNWAVNLMADVAQLESVNKMNARNIAMVFAPNMTQMADPLTALMYAVQVMNFLKTLIVKTLREREDAIVEAGPASRFDHFDENNGPHCSFQRTVDETDEVNMEEQDFSVKEPTLESHENICHNDLTSESQSQINVSEEKSGVSVVESGARVVQASKSRNSQSSNSSQKKGARKSVEKSKGSTIVSRLNSRNERIEAWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BZ36</accession>
    <name>A0AAD5BZ36_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UspA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_030178</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010344">
        <property type="protein sequence ID" value="KAI7731990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BZ36"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23659">
        <property type="entry name" value="USP_At3g01520-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31964">
        <property type="entry name" value="ADENINE NUCLEOTIDE ALPHA HYDROLASES-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31964:SF122">
        <property type="entry name" value="OS02G0760500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="160"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="227"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="UspA" evidence="2">
        <location>
            <begin position="9"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7731990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="259" mass="28646" checksum="1F54257585DDD0C7" modified="2024-05-29" version="1">MGEKQEQLRKVVVAVDGSEESMKGLQWALDNVRLHPTEGSLVIIHVQSPPSIAVGLNPGSIPFGAGPSDVEVPAFTAAIEAHQRRITEAIITHAFNICQHNNVYEGEVKSEVVIGDPKDKICEAVEELHADLLVMGCRSFGPIKSFNGGFWTKYESINNLLYIVCTLQALLLSPNIGVVFIEPHYISIGIYENQSIRDYIKCISGSSRAWRCKILTSFGFLLVKHEAYVELCCFLSSSNILVFFMALIVTLSFRMTPLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BZZ2</accession>
    <name>A0AAD5BZZ2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_015351</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010124">
        <property type="protein sequence ID" value="KAI7732873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BZZ2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042131406" evidence="2">
        <location>
            <begin position="21"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="70"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7732873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="103" mass="10228" checksum="7101E5896CE6FFEC" modified="2024-05-29" version="1">MASILKGGGLALSLIILIVASSTFDTISLADARTHARQLANRCQDPGQSDLRGCGGGGGGKGKGGSGGQSPRPGISHGPSPGSSHCKKGCCGMSKKQLCECCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C304</accession>
    <name>A0AAD5C304_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_004065</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009730">
        <property type="protein sequence ID" value="KAI7734416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C304"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36074">
        <property type="entry name" value="ISOPENTENYL-DIPHOSPHATE DELTA-ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36074:SF1">
        <property type="entry name" value="ISOPENTENYL-DIPHOSPHATE DELTA-ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7734416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="336" mass="36997" checksum="8E4AF37DBDE1FD40" modified="2024-05-29" version="1">MAGIALLMDLLKKNPNINGQTLHSSGLYSATLAASSSAAAYVASGIPFASSPLFGFGGKRFAYCDAGATPPLLGEDYLSSLRTASETIFRHDTLNYRTKEYYIEQKPLFSAFQPKSFALTTLRSFLLFYLPVLVPKVEDDNDFLPDSSDEHHVDLIVPFKKSVKQILRETSVVTTRRVLERLAVHHFSQRAAWKLLKDVPKSAMRKANRGMPFHTYFFCVSRTTIRGHFLGVAASWLVQVGIECYRFARDILKSNQEEDDVDADFDQSERVKVLGKKVYGVTVRCGASLVFASVGAGIGATLFRPSSGQSIGCLLGDMAGPIIVSFCFSNVAHLEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C4D6</accession>
    <name>A0AAD5C4D6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ENT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_025083</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009694">
        <property type="protein sequence ID" value="KAI7734555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C4D6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050832">
        <property type="term" value="P:defense response to fungus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20404">
        <property type="entry name" value="Tudor_Agenet_AtEML-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000088">
        <property type="entry name" value="Protein EMSY-LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1240.40">
        <property type="entry name" value="ENT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033485">
        <property type="entry name" value="EMSY-LIKE_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005491">
        <property type="entry name" value="ENT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036142">
        <property type="entry name" value="ENT_dom-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33432">
        <property type="entry name" value="PROTEIN EMSY-LIKE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33432:SF28">
        <property type="entry name" value="PROTEIN EMSY-LIKE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03735">
        <property type="entry name" value="ENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158639">
        <property type="entry name" value="ENT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51138">
        <property type="entry name" value="ENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENT" evidence="4">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="40"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="230"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="334"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="41"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="74"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="234"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7734555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="357" mass="39122" checksum="59C7159D491E1AEE" modified="2024-05-29" version="1" fragment="single">EKEGLITELRKELRLSNEEHRELLLKVNSDDVIRRIREWRQSSGLQPGMVNTSQAVHDPLPSPSVSGTRKKQKMNSSGPSQSFGVPPQHVPLPTSSSARRGPVPGSKGKNKKSLPLFWHGQMMGGVSSIKPQHPPSGSTGRAQLGNRVTPGVHANGAAETSSFSSLIGRKVRTRWPDDNNFYEATITDYNPNEGRHALVYDMYTTNETWEWVNLSEISPEDIQWVESEEPGIPQKGGYGGSGRGVGRPVGRDSGPGGGRGRGMTKGQTRKEFPPSQNGIGKKRLDDIQLLNTDTLIKEVERVFGSGHPDPLEMEKARKVLKEHEQALADAIARLADISDGDSDEGGRSMRGQPMDRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C4J7</accession>
    <name>A0AAD5C4J7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">ATP-dependent zinc metalloprotease FTSH, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">M8C21_026333</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">AA19_3_7</strain>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
            <orientation evidence="2">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the peptidase M41 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the N-terminal section; belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009675">
        <property type="protein sequence ID" value="KAI7734645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C4J7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045037">
        <property type="term" value="P:protein import into chloroplast stroma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19501">
        <property type="entry name" value="RecA-like_FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000001">
        <property type="entry name" value="ATP-dependent zinc metalloprotease FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.760:FF:000002">
        <property type="entry name" value="ATP-dependent zinc metalloprotease FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000195">
        <property type="entry name" value="ATP-dependent zinc metalloprotease FTSH 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.760">
        <property type="entry name" value="Peptidase M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01458">
        <property type="entry name" value="FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005936">
        <property type="entry name" value="FtsH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000642">
        <property type="entry name" value="Peptidase_M41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037219">
        <property type="entry name" value="Peptidase_M41-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01241">
        <property type="entry name" value="FtsH_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23076:SF97">
        <property type="entry name" value="ATP-DEPENDENT ZINC METALLOPROTEASE YME1L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23076">
        <property type="entry name" value="METALLOPROTEASE M41 FTSH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01434">
        <property type="entry name" value="Peptidase_M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140990">
        <property type="entry name" value="FtsH protease domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="15" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="18">
        <location>
            <begin position="361"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00070124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAI7734645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="779" mass="85422" checksum="A189F466AE5B0526" modified="2024-05-29" version="1">MTSLQAILLFKPPISPHPKPLIRLHQQNQSFFCPSTKTTLFSPKPIKKQPFFCTLHQENTNPSSDSSSLTNNNNNEVVVEGSTEKVNGGVEDKGGEVGKGKLVNFPVVVFLMGWFVKIKSGFERMLLSEWFSWWPLWRREKRLEMLVAEADADPKDAAKQTALLVELNKHSPESVIKRFEQREYAVDSKGVVEYLRALVTTNTISEYLPDDQSGKPSTLSALLEELKERASGNLDESFLSPGISERQPLHVMMVDQKASSRSHFAQEVISTILFTVAVGLIWVMGAAALQRYIGGLGGMGTPGVGSSSSYSSKDLNKEIMPEKNVKTFKDVKGCDDAKQELEEVVEYLKNPGKFTRLGGKLPKGILLTGAPGTGKTLLAKAIAGEAGVPFFYRAGSEFEEMFVGVGARRVRSLFQAAKKKAPCIIFIDEIDAVGSTRKQWEGHTKKTLHQLLVEMDGFEQNEGIILMAATNLPDILDPALTRPGRFDRHIVVTNPDVKGRQEILDLYLHDKPLAEDVDVSAVARGTPGFNGADLANLVNVAAIKAAVEGSDKLNAAQLEFAKDRIIMGTERKTMFLSEDSKKLTAYHESGHAIVALNTDGAHPIHKATIIPRGSALGMVTQLPSNDETSVSKKQLLARLDVCMGGRVAEEIIFGSDYITTGASSDLQSATEIAQYMVSSYGMSDAIGPVYLKERPGSEMQARIDAEVVKLLKDAYARVKSLLKKHEKSLHALANALLEHETLNAEEIKRVLGPNYPEGKLVIPDQEVLQEQEEGELVLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C6Y3</accession>
    <name>A0AAD5C6Y3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Patatin</fullName>
            <ecNumber evidence="4">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_018301</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Lipolytic acyl hydrolase (LAH).</text>
    </comment>
    <comment type="domain">
        <text evidence="4">The nitrogen atoms of the two glycine residues in the GGXR motif define the oxyanion hole, and stabilize the oxyanion that forms during the nucleophilic attack by the catalytic serine during substrate cleavage.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the patatin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="4"/>
    <dbReference type="EMBL" id="JAMZMK010009273">
        <property type="protein sequence ID" value="KAI7736443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C6Y3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047372">
        <property type="term" value="F:monoacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004620">
        <property type="term" value="F:phospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32176:SF80">
        <property type="entry name" value="ACYL TRANSFERASE_ACYL HYDROLASE_LYSOPHOSPHOLIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32176">
        <property type="entry name" value="XYLOSE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 4" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="2 3 4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PNPLA" evidence="5">
        <location>
            <begin position="17"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXGXXG" evidence="3">
        <location>
            <begin position="21"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="3">
        <location>
            <begin position="59"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="61"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="178"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7736443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="260" mass="28651" checksum="C4297A0FB2CFD493" modified="2024-05-29" version="1" fragment="single">PRSPLQSPKYGNLITILSIDGGGVRGIIPGVILEFLETELQALDGENARLADYFDVIAGTSTGGIVTAMLTTPNEEGRPVFSAKDVKDFYRKHCPKIFPHDRVGEALTNVVITSFDIKLMQPVIFSSYEVLVAISEVTKEILSGSPDFFPIKPMEYGRFLVLSLGTGTSKSKEHDATDGDIVDYHLSTVFQALHSAENYLRIQDDTLEGDLTKLDLATDKNLENLVEVGEKLLMKQVSRVNLGIGIYEPYHNTTNEMALK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C757</accession>
    <name>A0AAD5C757_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">PRA1 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_000535</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 7">May be involved in both secretory and endocytic intracellular trafficking in the endosomal/prevacuolar compartments.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 7">Membrane</location>
            <topology evidence="2 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the PRA1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009435">
        <property type="protein sequence ID" value="KAI7735773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C757"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004895">
        <property type="entry name" value="Prenylated_rab_accept_PRA1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19317">
        <property type="entry name" value="PRENYLATED RAB ACCEPTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19317:SF72">
        <property type="entry name" value="PRENYLATED RAB ACCEPTOR PRA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03208">
        <property type="entry name" value="PRA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="65"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="117"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="143"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7735773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="183" mass="20819" checksum="0DC8F7BBE70B01E0" modified="2024-05-29" version="1">MTTYGTIPSSSLERINIEYLSRAKARIKSGLGTRRPWKQMFNLASINFPHSLSVAFSRIKTNVGYFRMNYVIIMLVILFLSLLFHPISLIVFVIMMAAWLFLYFLRDEPLIIFHRTIGDRAVLVVLFVVTIVLLLLTDVTWNIVSSVLIGLFVVVVHAALRNTDDLVDEDRVESGDYFVASSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C7Z2</accession>
    <name>A0AAD5C7Z2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Histone-lysine N-methyltransferase, H3 lysine-9 specific SUVH1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_032323</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009168">
        <property type="protein sequence ID" value="KAI7736892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C7Z2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042054">
        <property type="term" value="F:histone methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.280.10:FF:000003">
        <property type="entry name" value="Histone-lysine N-methyltransferase, H3 lysine-9 specific SUVH5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.280.10">
        <property type="entry name" value="SRA-YDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025794">
        <property type="entry name" value="H3-K9-MeTrfase_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051357">
        <property type="entry name" value="H3K9_HMTase_SUVAR3-9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003616">
        <property type="entry name" value="Post-SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007728">
        <property type="entry name" value="Pre-SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036987">
        <property type="entry name" value="SRA-YDG_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003105">
        <property type="entry name" value="SRA_YDG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45660">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SETMAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45660:SF13">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SETMAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05033">
        <property type="entry name" value="Pre-SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02182">
        <property type="entry name" value="SAD_SRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00468">
        <property type="entry name" value="PreSET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00466">
        <property type="entry name" value="SRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50868">
        <property type="entry name" value="POST_SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50867">
        <property type="entry name" value="PRE_SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51575">
        <property type="entry name" value="SAM_MT43_SUVAR39_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51015">
        <property type="entry name" value="YDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="2" id="KW-0158">Chromosome</keyword>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="YDG" evidence="13">
        <location>
            <begin position="227"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="domain" description="Pre-SET" evidence="11">
        <location>
            <begin position="450"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="domain" description="SET" evidence="10">
        <location>
            <begin position="514"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="domain" description="Post-SET" evidence="12">
        <location>
            <begin position="653"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="23"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="80"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="46"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7736892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="669" mass="73129" checksum="ADE7589BD17339CB" modified="2024-05-29" version="1">MHSAVLTVKPLRCLAPIFPSAPDPSAPPSAQYASIPPNGPFPPGATPFFPFFAANGPQRPGQPRPFTIPSPVPLNSYKTPGSAAGNGNIGTSRGASRGRTIVDEDGNSVSDGYDEAGNEFNDKTPVTRRKVRSRSGIPVSDTDVAIDSLAEDMLRAFSVAEIDTSRQANSDKELVDRVLIVFNLLRRKITQIEEKTAVPGVARRPDLRAGTLCMSKSVRANTRKRIGPVPGVEIGDIFFFRMELCLAGVHAPSMAGIDYMGVKVAGEDDSMAVSIVSSGGYEDEGDDGEVLIYSGQGGVQRKDKQFSDQQLVRGNLALEKSLHKANEVRVIRGLKDFASPTGKVYVYDGLYKIHESWVEKGKSGCNIFKYKFVRVAGQAEAFTLWKSIQQWKDGVTSRPGIILPDLTSGAESLPVCLVNDVDDEKGPSYFSYTPSLKYTKAFPSTKPSVVNCNCCTGGCQSNSDCPCVQRNGGQIPYTSLGVLQSHNTLIHECASMCICPVNCRNRISQAGLKMRLEVFKTKDKGWGLRSWDPIRSGSFICEYAGVLISENGIDSEDNYIFDATRTFEPLEAQPSEEPVKFPFPLIISAKNEGNVARFMNHSCSPNVYWQPIIRENQDESYFHVGFFAIKHIPPMQELTFDYGMLLAGKAGPRKKKCLCGSLKCKGVFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C8D8</accession>
    <name>A0AAD5C8D8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein SERAC1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_011843</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009272">
        <property type="protein sequence ID" value="KAI7736448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C8D8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48202">
        <property type="entry name" value="ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48202:SF1">
        <property type="entry name" value="ALPHA_BETA-HYDROLASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="209"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="772"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="215"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1071"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7736448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1071" mass="117535" checksum="4DC06102D745CF85" modified="2024-05-29" version="1" fragment="single">QLVNEIEEVIDRSKTSLTKLINSSKNTGIAATVLWKSLHSVLSSANHEVRLGFEVRVAALLADIVAASESRRAAIVGAGGGVVVDWLLEKVAVGGGGSCGTQAEAARALAYLVADPNVCEKVVGRDHAVTNLLRFIFFAQPNQSRKQPRRSSVDYSDPTKGRSMLVAAIMDIVTSNCDDVDMVRLKPALSGAAAMRDIAAALQVIEEGGMHMDDPPGDEQEDDDGGPGLKGIGIKVLGGTSVVGLSTSNGSSMELDKSYKSTPKILQYKKLNDNSAAVIPGLWDDLHSQHVAVPFATWALANWAMASDVNRSHIQELDRDGHAVMSALVAPERSVKWHASLVARLLLEDQNLHINDYISDWSSTLLKTVYQASKTNDISLTRVALTAFLSSIERSPQARRVVIEKGLDLMRETAKRTIDHNSVQESLAKGLELLCSEDMHLSLEEGQKWSGILLPWVFRENSSDAIRSSAITILSRICEDYGPSSIPISQGWLDIMLSDILKLRKLSLKGSAQPKDKVKTQIDQSNVVSGNQSANQLANAVVNLALNQSGTESINGYLSPLADFLGLEPFIGIYRNLKKDNIPKVNAAESALATLKGIKALTEICSDDLFCQNKIIDYGVVSLLRRFLLNDDYEKLAAIEAYDASRDMETKDKSVNVHGESSVQDARGSSSVRVPPVAHVRRHAARLLTLLSVHPKVKKLIRDDEAWCHWLEECANGKISGNNDLKTQSYARATLLNIFCDDDNSGDKDTRCARYTEMIFLINPDMPHWECRKGRHTSTEKQNPIDVSDDISPNSDSRLESQLGDPSLDVIFVHGLRGGPFKTWRLSECKSSSKSGLVEKIDEEAGKQGTFWPGEWLSADFPHARMFSLKYKTNLTQWSGSSLPLQEVSSMLLEKLLAARIGDRPVVFVTHSMGGLVVKQMLHQASAENRGNLVKNTVGVVFYSCPHFGSKLADMPWRMGYVLRPAPSIGELRSGSTRLVELNDFIRDLHKKGSLDVLSFCETKVTPIVEGYGGWAFRLEIVPIESAYPGFGELVVLESTDHVNSCKPISRADPSYSETLQFLHKLRAAYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CA52</accession>
    <name>A0AAD5CA52_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_018931</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008867">
        <property type="protein sequence ID" value="KAI7738151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CA52"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33738">
        <property type="entry name" value="EMB|CAB82975.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33738:SF1">
        <property type="entry name" value="PLANT_T7H20-70 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7738151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="120" mass="13759" checksum="F278EE4696BF1AE1" modified="2024-05-29" version="1" fragment="single">SADNEEKNRNPKAHTSPFYQQETSLPCPLSSSIYYGGQDIYTNPSITKNNPGHNTPKMEKMMGVLQEEIGGRDHSITKTTLRTYLYDETKSRLLGKFCFPMVVIDELLLFRLFSLCIHRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CAS6</accession>
    <name>A0AAD5CAS6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_021330</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008936">
        <property type="protein sequence ID" value="KAI7737858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CAS6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7737858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="198" mass="21633" checksum="E6705C2607D90BDD" modified="2024-05-29" version="1">MRCFSCSLSGISSDSIVLGDIPICNNTVDGVGIMINLTGLTRWQPFATWIIRVSCKCLTEGALNVEGLINMPFVLAACKLLCYRDAALQMACFDLVRILGAVLNDDMIDIIPSENMILSISTILSEEEDGLPVFRDITYDASLGGCLQSLYSCCADDVIKLTAANIISVFSDSMQKTESPDLQVNGKDCRLQFIRKQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CB43</accession>
    <name>A0AAD5CB43_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_015138</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008920">
        <property type="protein sequence ID" value="KAI7737945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14798">
        <property type="entry name" value="RX-CC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001091">
        <property type="entry name" value="Probable disease resistance protein At1g61300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056789">
        <property type="entry name" value="LRR_R13L1-DRL21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038005">
        <property type="entry name" value="RX-like_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041118">
        <property type="entry name" value="Rx_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058922">
        <property type="entry name" value="WHD_DRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766:SF47">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766">
        <property type="entry name" value="PLANT BROAD-SPECTRUM MILDEW RESISTANCE PROTEIN RPW8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25019">
        <property type="entry name" value="LRR_R13L1-DRL21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18052">
        <property type="entry name" value="Rx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23559">
        <property type="entry name" value="WHD_DRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0611">Plant defense</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance N-terminal" evidence="7">
        <location>
            <begin position="10"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="NB-ARC" evidence="6">
        <location>
            <begin position="226"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein winged helix" evidence="8">
        <location>
            <begin position="434"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="domain" description="R13L1/DRL21-like LRR repeat region" evidence="9">
        <location>
            <begin position="567"/>
            <end position="694"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF18052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF23559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF25019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7737945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="810" mass="91563" checksum="9868666D9B243182" modified="2024-05-29" version="1">MGEVVVSAIVDEVVGRITSELIKEFRLIWGFKSDILSLKEDFSLNKAYLKDALEKQNKAEAVQEWLKLLKSAIMEAENVLDEISTQALMQRLLKEMGIERGIKRGAKYKVRALFSKVVFRVKAAHKVKAIRAKLDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXATSHVDEDSAGKMPNRETSSLAESILGRDEESEIVVENICKNGIGKYENGEILVYGIWGMGGIGKTTLAKLVYHHEKVKQNFPLRFWVYVSQKFEVNEIIKTIIESIDGSKCELTQLETLQGELQKRLTGKKFLIVLDDVWLEVEEKAKWDELSKTLSCGEEGSIVVMTTRSSTTSRMMAKVGELQHELGCLSKDNSWLLFSKFAFEQGREGDAISLLEPIGREIVEKCSGLPLALKTFGSLMWSKKTTRDWEHVKGYEMKKDELIQLWAANGFIPPIEGKSLYMFGEEILNCLVWRSFFQLVHTQKAYAKDPYVYKMHDLMHDMALHVMGDDCLVIESTEGLRHMVNLQCLDISYCSNLRRLPAGIKELTRLRILSRFPIDKEGGAKIGELGDLDLLEEGLEISGIGNVEGFSEAESANLKCKTNLSVLKLSWTYLGLGLPRYKCDEKEVLAGLEPNPGLKALKISLFMDKIISPSWMVNLKNLIKISFSSCKKCEHIPPLGRLPNLRVIKLKQMICLKGFHEDDTNMPGDNPNIFPSLQTLNISNCESLVSLPRNLPKLRVLRLWRCDELVSLPDEILKDLNQLDIYGGKHLIARYKQKMGEDWPKVSHIPDVKIDHALWIGLASEENNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CBA4</accession>
    <name>A0AAD5CBA4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_009771</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Tim17/Tim22/Tim23 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008846">
        <property type="protein sequence ID" value="KAI7738240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CBA4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042721">
        <property type="term" value="C:TIM22 mitochondrial import inner membrane insertion complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030943">
        <property type="term" value="F:mitochondrion targeting sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045039">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039175">
        <property type="entry name" value="TIM22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14110">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14110:SF0">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02466">
        <property type="entry name" value="Tim17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7738240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="196" mass="20817" checksum="F4213C18B52ED083" modified="2024-05-29" version="1" fragment="single">WNFREINLTWKSSTIELILDSPLPATLLFRCMTEDSSNTAAPANANETEKPAIEPIRLPTIEEVRGQDIWNNCAVRSVASGIMGGGLGLAMGLFLGALDNPIHQDTMTGRQLFVYTAKQMGGRSWNSCKAFAVMGLVFSAAECARAKHDITNTAIAGCVTGGAISAKTGGPKGACFGCAGFAAFSVAIEKFLDRHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CC58</accession>
    <name>A0AAD5CC58_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_017508</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008641">
        <property type="protein sequence ID" value="KAI7739278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CC58"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7739278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="130" mass="14291" checksum="B40145C446E833BC" modified="2024-05-29" version="1" fragment="single">WGVEDGGDGDTSHVSLRWILATYEDMKRSNICDCWRAWVTDAGTVEYWWSTTVNNNNNNLDKCGCGDLYAQASGSFIIDLTNNFQLDFIATGCEDGTVRYSSGGDNWSASKLLGQRVGSSAVRSLCTVQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CCQ7</accession>
    <name>A0AAD5CCQ7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_001014</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the MCM family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="1"/>
    <dbReference type="EMBL" id="JAMZMK010008619">
        <property type="protein sequence ID" value="KAI7739472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CCQ7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042555">
        <property type="term" value="C:MCM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000347">
        <property type="term" value="C:THO complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990518">
        <property type="term" value="F:single-stranded 3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902969">
        <property type="term" value="P:mitotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018525">
        <property type="entry name" value="MCM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630:SF43">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00847">
        <property type="entry name" value="MCM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MCM C-terminal AAA(+) ATPase" evidence="5">
        <location>
            <begin position="28"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7739472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="137" mass="15345" checksum="C409D88474C6F0F4" modified="2024-05-29" version="1">MSEENQVLQRQLMEKRDRELGNMKSYLVAKEPETGEFCIESGALMLADNGVCCIDEFDKMDVRDQVAIHEAMEQQIISITKTGIQATLNARTSILAAANPTGSRYDKTKPSKYDVALPPAILSRFDLVYIMIDDPDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CCU9</accession>
    <name>A0AAD5CCU9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Myosin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_017358</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008607">
        <property type="protein sequence ID" value="KAI7739547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CCU9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140">
        <property type="entry name" value="MYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140:SF825">
        <property type="entry name" value="MYOSIN ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0505">Motor protein</keyword>
    <keyword evidence="3 6" id="KW-0518">Myosin</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myosin motor" evidence="7">
        <location>
            <begin position="40"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7739547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="254" mass="28179" checksum="A2A879005FB4268E" modified="2024-05-29" version="1" fragment="single">VHINRVYKKRIDRLNSLRSKLRQVSELSSSDKSSNTKSKAGPILVAINPFKTIPLYGDDYIEAYKRKEIDSPHVYVTDTAIREMVRDEVSQSIVISGESGAAKTETAKIAMQYLAAVRGGSGIEYEILETNPILEGFGNAKTLRNDNSSRFGKLIEIHFSEIGRISGASIQTCNMAHFSDVITSKKLILLEKELHLLLEEALDIVHVSKEDQENVFAKLAAVLWLGNITFSVVDSNNHVQPVIDEALLTVAKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CEJ4</accession>
    <name>A0AAD5CEJ4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_024279</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008565">
        <property type="protein sequence ID" value="KAI7739815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CEJ4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7739815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="160" mass="18569" checksum="8D27D3CDD08F63C5" modified="2024-05-29" version="1">MQEVRAVENVTLARNLMLELQVLLEQWKENSETSKARMKQDKATVPEQGKVENHRAKRDTKMKCNFGERMTRIESYWKDLNLQVGKGYNNTCSVISLLRKIEKVLKEVPRSHWTKLQARFSSLRAEAETIIDDTTDQMKMQVDKKPNLFRELGTSSHGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CEP7</accession>
    <name>A0AAD5CEP7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GDSL esterase/lipase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_004105</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008669">
        <property type="protein sequence ID" value="KAI7739131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CEP7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01837">
        <property type="entry name" value="SGNH_plant_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051058">
        <property type="entry name" value="GDSL_Est/Lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035669">
        <property type="entry name" value="SGNH_plant_lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45648">
        <property type="entry name" value="GDSL LIPASE/ACYLHYDROLASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_4G14700)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45648:SF179">
        <property type="entry name" value="SGNH HYDROLASE-TYPE ESTERASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7739131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="334" mass="36692" checksum="03E2216C3FE585DD" modified="2024-05-29" version="1" fragment="single">DSGNNNYLATTARADAPPYGIDHPSHRATGRFSNGFNIPDLIRYLLIDLSSTTGQRIGSEPTLPYLSPELNGDKLLRGANFASAGVGILNDTGVQFVNIIKAPAQLDYFKQYQQRVSALIGPEYTKQLVNNALVLITLGGNDFVNNYYLVPLSARSRQYKLQDYVPFIISEYRKILMRLYDLGARKVLVTGTGPLGCVPAELATRSRNGECAAELQYAASLYNPQLDDMLTSLNQELGSHVFVGVNTRKMHNDFMTNPGDYGFVTAKIACCGQGPYNGIGLCTAMSSICPNRDVYAFWDAFHPSEKASRLIVDQIMSGTTEYMKPMNLSTILAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CG51</accession>
    <name>A0AAD5CG51_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_029311</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008543">
        <property type="protein sequence ID" value="KAI7739909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CG51"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35318:SF7">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35318">
        <property type="entry name" value="BNAA10G08410D PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7739909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="113" mass="12679" checksum="04F3AC1945AF9CD8" modified="2024-05-29" version="1">MKFFFPELGSCFGSTVVPPTTEIICTDVSTTRRRVRHGKFSKNWKPELSAIVEDGGTMNARRQSHESVILEKKSLNKSRSARKTRSHSYSSGDYGKFTQAMAIPAFSPTPFVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CGU5</accession>
    <name>A0AAD5CGU5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mediator of RNA polymerase II transcription subunit 9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_022596</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008180">
        <property type="protein sequence ID" value="KAI7741518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CGU5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038790">
        <property type="entry name" value="Med9_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37188">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37188:SF1">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7741518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="78" mass="9001" checksum="C9ECE24B191A4381" modified="2024-05-29" version="1">MRLRMEPEINILIHCELSSNFEKCQQLLNTISGSIATKAATVEGQKHKVEEADQMLNQRRELMAKYRNSVEELTKPDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CH46</accession>
    <name>A0AAD5CH46_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Nucleosome assembly protein 14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_022595</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">May modulate chromatin structure by regulation of nucleosome assembly/disassembly. Could function together with B-type cyclins in the regulation of microtubule dynamics.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the nucleosome assembly protein (NAP) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008180">
        <property type="protein sequence ID" value="KAI7741517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CH46"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1500:FF:000001">
        <property type="entry name" value="Nucleosome assembly protein 1-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1120.90:FF:000005">
        <property type="entry name" value="Nucleosome assembly protein11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1500">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1120.90">
        <property type="entry name" value="Nucleosome assembly protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037231">
        <property type="entry name" value="NAP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002164">
        <property type="entry name" value="NAP_family"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11875">
        <property type="entry name" value="TESTIS-SPECIFIC Y-ENCODED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00956">
        <property type="entry name" value="NAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143113">
        <property type="entry name" value="NAP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="288"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="292"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="351"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00056035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7741517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="361" mass="40973" checksum="01B7819EA5147336" modified="2024-05-29" version="1" fragment="single">GFSPAMANNKLDISDLTASLPAATALSVEDRVGLMSSLKNLTPAVRKRVEVLRDIQSEHDDLEAKFFEERAALEMKYQKLYQPLYSKRHDIVNGVVEVDGLKDEPATAQADGNSKEEKGVPDFWLTAMKTHELLAEEISERDEEALKYLKDIKWCRIEDPKGFKLEFFFDTNPYFKNSVLTKVYHMIDEDEPILEKAIGTSIEWLPGKCLTQKVLKKKPKKGSKNAKPIIKTEDCESFFNFFNPPPVPEDADDLDEEEAEELQNRMEQDYDIASTIRDEIIPHAVSWFTGEAAEDDESDGTEDDDEEISDEDEHDDEDEDDEDEDDEVEEESKSNRKTKKGGLPNGAAGEQGEKPPECKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CJ40</accession>
    <name>A0AAD5CJ40_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_032678</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007835">
        <property type="protein sequence ID" value="KAI7743013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CJ40"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47697">
        <property type="entry name" value="OS03G0340700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47697:SF1">
        <property type="entry name" value="OS03G0340700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="189"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="293"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="382"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="189"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="293"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="310"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7743013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="530" mass="54166" checksum="E2F135BD5C6051DE" modified="2024-05-29" version="1">MSSNYGKSTKSGSLNTFDFDLGLNSNRSTPLNAQKPNNNNNNSYTQPKPYTTTSSWQPNKPSWTHQPAAPTQSSTLGASLNGPTSMVGDITGRSWTSSVPQHGSSIGIANKNPNLFSDLLGSNLGVGKGGSSGGGNVPLKNAAPVSSGAGSRSAYSMGGMADSLPKFGNTTNSGGGWGSGSGSSQGIGNYNSSGYGSKSQNLGGPSMKSSSVSGAGVGVGGGMKSNKDPFGSLVDFSSKPAAGMKAGSKPTAAKTSGDDVFGTFQNASKPSATGSGIGSQVKLDDFGFGQPPVQSSGMGSQSKLDDFGFGQPSVQPSSVGSQTKMDDFGFGGMQSQPPVQPSGGNDFDMLFTSSSGGNASKGPETFVNEQFAGAEDWGFESDIGGGVGDVGGTTELEGLPPPPAGVTSSSAKSKGMDNYKAGQYPDAIKWLSWAVILLEKAGDNAGTMEVLSSRASCYKEVGEYKKAVADCSKVLEQDGKNVSVLVQRALLYESMEKYKLGAEDLRTVMNIDPGNRVARSTIHRLTKLAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CK52</accession>
    <name>A0AAD5CK52_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_013601</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007813">
        <property type="protein sequence ID" value="KAI7743107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CK52"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="115"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7743107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="139" mass="16266" checksum="91B05134F881BED0" modified="2024-05-29" version="1" fragment="single">LPEPKSCEDREQEVSISLKRGHHIRLYAGEEGEQGGKNGGWFTRRRWDAVCFRVDLRRRCGEVGRWQLRGSAVVFSTLRLVADDDGYNTMKTDYLQPYYMSGHTNWATWFPGDELMVMTTVSILLYMFIYCCLNFFPVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CKI9</accession>
    <name>A0AAD5CKI9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Cation/H+ exchanger transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">M8C21_000736</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">AA19_3_7</strain>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>K(+)(in) + H(+)(out) = K(+)(out) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:29467"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>Na(+)(in) + H(+)(out) = Na(+)(out) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:29419"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007713">
        <property type="protein sequence ID" value="KAI7743537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CKI9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009941">
        <property type="term" value="C:chloroplast envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015386">
        <property type="term" value="F:potassium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015385">
        <property type="term" value="F:sodium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051453">
        <property type="term" value="P:regulation of intracellular pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098719">
        <property type="term" value="P:sodium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018422">
        <property type="entry name" value="Cation/H_exchanger_CPA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10110">
        <property type="entry name" value="SODIUM/HYDROGEN EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10110:SF86">
        <property type="entry name" value="SODIUM_HYDROGEN EXCHANGER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0630">Potassium</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0915">Sodium</keyword>
    <keyword evidence="11" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="41"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="119"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="16">
        <location>
            <begin position="8"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="422"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="458"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="478"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="506"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAI7743537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="516" mass="57894" checksum="B7DE47059CFA5517" modified="2024-05-29" version="1">MLNFREMVAYIANTLIFILSGVVIAEGILGGDGILKHEKNVWGYLILLYVLLLLSRAIVVGSLYPLLSYCGYGLDWKEAIVLVWSGLRGAVALSLSLSVKATIQRHIIIHYSGNRNTGIFVFFTGGIVFLTLIVNGSTTQFVLRMLKMDKLSAAKTRILEYTKYEMMSKALEAFGDLVDDEELGPADWPTVKKYIPSLQDSEGERMHPHTSIENDNNVEHMHLSDIRIRFLNGRPFCVLIYLPLCIPYLIPHGVRCASCLLGDVRASYYKFLQSTTIPRKLVTYFTVERLESACYISAAFLRAHRIARQQLHDFIGDSEIALAIINESEREGEEAKKFLEDVRTTFPQWDSKKIKKRYSLHPTFAHGSTLGLYEVLTGKRYMCDIITDSVVLGFFIEAEKILSVLGTDDAVDDFLWQISNERHSPHNGAAKPSETPSHSKSYPRVPPTERRGLLSVRSEGSTTVRKTVNTGSESLIGPDDRNDGHEMDYSSDESGGEDEHIVRIDSPSTLSFSRAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CKM5</accession>
    <name>A0AAD5CKM5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_006659</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Phosphate:H(+) symporter (TC 2.A.1.9) family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Proton-dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007739">
        <property type="protein sequence ID" value="KAI7743362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CKM5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17416">
        <property type="entry name" value="MFS_NPF1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000130">
        <property type="entry name" value="Protein NRT1/ PTR FAMILY 3.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000109">
        <property type="entry name" value="POT_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11654">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00854">
        <property type="entry name" value="PTR2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="49"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="105"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="151"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="197"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="223"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="352"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="394"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="435"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="477"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="520"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7743362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="567" mass="63014" checksum="F1B6955DACE731ED" modified="2024-05-29" version="1">MSPPSSLVENEVGKAHHHHEPPQPKVVQRKPGGWKSMPYILGNESFERLASVGLLANFTVFLMTIFHMNQVSASNLINIWYGINNFAPLIGAFISDAYVGKFWVIAFSSFATLMGMVTMTLIVALPKLHPPSCAHQQALLNQCQGPTTRQFGFLALALGFLSVGTGGIRPCSLPFGVDQFDPTTNEGKKGIDSFFNWYYTTFTIIMLIAMTVVVYIQDSVSWVLGFGIPTILMACSIVLFFVGTKKYIYVKPEGSIFSGMAQTLAAAYKKRNLEVEKTMLYDPPITKGTYQLQKLPLTHQFRYLNKAAIILDGETNPDGSRISSWNLATLIQQWTFTVSQALKMDRHLGPHFQIPAGSIGVISMITIGIWLPIYDRIIVPFLRKITKIESGITLLQRIGIGIVFSILAMIVAAMVEKKRRDSAISHNQPDGIAPLSVMWLAPQLILMAFAEVFNLLGQLEFYYKEFPDNMKSMGTAMFCVMAGVANYLSSALVTTVHKVTGKHGQPNWLAADINAGRVDYFYYVIAGLGVLNMGYYLIVSSQYQYKKKIQGNEEESELDIELNASKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CLS9</accession>
    <name>A0AAD5CLS9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Photosystem I reaction center subunit psaK, chloroplastic</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">PSI-K</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Photosystem I subunit X</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">M8C21_032020</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">AA19_3_7</strain>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast thylakoid membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PsaG/PsaK family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007631">
        <property type="protein sequence ID" value="KAI7743909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CLS9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009522">
        <property type="term" value="C:photosystem I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015979">
        <property type="term" value="P:photosynthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.286.40:FF:000001">
        <property type="entry name" value="Photosystem I reaction center subunit psaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.286.40">
        <property type="entry name" value="Chlorophyll a-b binding protein like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035982">
        <property type="entry name" value="PSI_centre_PsaK_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000549">
        <property type="entry name" value="PSI_PsaG/PsaK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023618">
        <property type="entry name" value="PSI_PsaG/PsaK_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016370">
        <property type="entry name" value="PSI_PsaG/PsaK_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017493">
        <property type="entry name" value="PSI_PsaK_pln"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03050">
        <property type="entry name" value="PS_I_psaK_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34195:SF2">
        <property type="entry name" value="PHOTOSYSTEM I REACTION CENTER SUBUNIT PSAK, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34195">
        <property type="entry name" value="PHOTOSYSTEM I REACTION CENTER SUBUNIT V, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01241">
        <property type="entry name" value="PSI_PSAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81563">
        <property type="entry name" value="Photosystem I reaction center subunit X, PsaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01026">
        <property type="entry name" value="PHOTOSYSTEM_I_PSAGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0602">Photosynthesis</keyword>
    <keyword evidence="7" id="KW-0603">Photosystem I</keyword>
    <keyword evidence="5" id="KW-0934">Plastid</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0793">Thylakoid</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00033430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00074570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAI7743909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="164" mass="16855" checksum="B975E2B6F1F64E39" modified="2024-05-29" version="1">MAINTTLLCGSCFKDLSINSIFSNPVIQVFSRSQMATAVMSSLPQFTGLKATSSSPSPVQSMVSLKPMKPKGKGALGVRCDFIGSSTNLIMVTSTTLMLFAGRFGLAPSANRKATAGLKLEVRDSGLQTGDPAGFTLADTLACGTVGHIIGVGVVLGLKNIGAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CN18</accession>
    <name>A0AAD5CN18_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">DEAD-box ATP-dependent RNA helicase 40</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_027074</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007403">
        <property type="protein sequence ID" value="KAI7744953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CN18"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00268">
        <property type="entry name" value="DEADc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044742">
        <property type="entry name" value="DEAD/DEAH_RhlB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="WW" evidence="8">
        <location>
            <begin position="15"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="10">
        <location>
            <begin position="338"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="369"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="45"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="143"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="6">
        <location>
            <begin position="338"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="49"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="143"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="179"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="509"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7744953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="509" mass="55706" checksum="DDB6B277EFD36567" modified="2024-05-29" version="1" fragment="single">GASDASGPRYAPPDPTLPEPWKGLIDGSSGVMYYWNPETNITQYEKPTAVPPQVPSGPPPVPSVPQPVSALGARTEQPNGIPGQQTPQMFPTPEQQMPPQQGNSIPQSYQGVNMEQSQSYQFTHHQPMPYMGYQQRPPNMMQPPQHLQQFPPQQGHRATPPVQNQHSDAIPNQPPPTQYGGSQFNMQQPSSFGQLQQSGVEYQQHGQQFQNQMGQQPNIPPVGSKMGFEDNPPGRGGNDQYFNAKNGGPAMTPHQPKLAAIPVARTQVNVPSVVPGGPVSHNMYGQATGGPPFMNNAMMRANPAVMVSPDAMNLSSVDMYRQKHDVTATGDNVPAPFMSFESTGFPPELLREIYAAGFAFPTPIQAQTWPIALQNRDIVAIAKTGSGKTLGYLIPAFMLLRHCNNNPQNGPTVVVLAPTRELATQIQDEAIKFGRSIRISCTCLYGGAPKGPQLKELEQGADIVVATPGRLNDILEMRKVDFRQYVEVVPEMEKQRRVEQILSSEERGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CP45</accession>
    <name>A0AAD5CP45_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Endoglucanase</fullName>
            <ecNumber evidence="10">3.2.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_029917</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>Endohydrolysis of (1-&gt;4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 8 10">Belongs to the glycosyl hydrolase 9 (cellulase E) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.4" evidence="10"/>
    <dbReference type="EMBL" id="JAMZMK010007322">
        <property type="protein sequence ID" value="KAI7745229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CP45"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008810">
        <property type="term" value="F:cellulase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001701">
        <property type="entry name" value="Glyco_hydro_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033126">
        <property type="entry name" value="Glyco_hydro_9_Asp/Glu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018221">
        <property type="entry name" value="Glyco_hydro_9_His_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22298">
        <property type="entry name" value="ENDO-1,4-BETA-GLUCANASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00759">
        <property type="entry name" value="Glyco_hydro_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00592">
        <property type="entry name" value="GH9_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00698">
        <property type="entry name" value="GH9_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8 10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4 10" id="KW-0136">Cellulose degradation</keyword>
    <keyword evidence="6 8 10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3 8 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 8 10" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycoside hydrolase family 9" evidence="11">
        <location>
            <begin position="7"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="116"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="167"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7745229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="196" mass="21091" checksum="C0D9CE46E7532EA5" modified="2024-05-29" version="1">MGKSPDLKSFQNNADAFICSLCPGTAHLQVQYSPGGLIFKAGGSNMQHVTSLSFLLLAYSNYLSHANHAVTCGGKSASPALLKDLARRQVNYILGENPMKMSYMVGYGPRYPQRIHHRGSSLPSVRTHPAHIGCKAGSRYFLSPDPNPNVLVGAVVGGPNTTDAFPDSRPFFQESEPTTYINAPLVGLLAYFAAHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CPT0</accession>
    <name>A0AAD5CPT0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_004492</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007220">
        <property type="protein sequence ID" value="KAI7745507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CPT0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7745507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="118" mass="13507" checksum="3A7AC0D1CD997313" modified="2024-05-29" version="1" fragment="single">KLQVNLHDEAQDVLRLCDVMETWDVELSSIRETLELKDAELLEHSENVAELEVTSHPPADERSRLHEQIVDLTEENSSLKDNHEWLLQCCITEVEHHVKRGSCTTEVIGELVLATKIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CU61</accession>
    <name>A0AAD5CU61_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Poly(A) RNA polymerase mitochondrial-like central palm domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_010205</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006939">
        <property type="protein sequence ID" value="KAI7746601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CU61"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031123">
        <property type="term" value="P:RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054708">
        <property type="entry name" value="MTPAP-like_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12271:SF134">
        <property type="entry name" value="NUCLEOTIDYLTRANSFERASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12271">
        <property type="entry name" value="POLY A POLYMERASE CID PAP -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22600">
        <property type="entry name" value="MTPAP-like_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81631">
        <property type="entry name" value="PAP/OAS1 substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Poly(A) RNA polymerase mitochondrial-like central palm" evidence="1">
        <location>
            <begin position="2"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF22600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7746601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="98" mass="10992" checksum="18934E682EA3412E" modified="2024-05-29" version="1" fragment="single">MRAKVPILKFVDTATGVECDISVGNKEGISKSLIIRFVTSIDERFQKLCFLMKAWARAHNINSPKDRTLNSVSIILLVAFHLQTRDPPILPPFSAILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CUL5</accession>
    <name>A0AAD5CUL5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_006592</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006836">
        <property type="protein sequence ID" value="KAI7747124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CUL5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7747124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="36" mass="4313" checksum="897DB968599B2CF7" modified="2024-05-29" version="1">MCYSKRQREDDDDDQHVLKEWVRGRSSCTSPTLTRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CVX1</accession>
    <name>A0AAD5CVX1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">60S ribosomal protein L10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_031856</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006753">
        <property type="protein sequence ID" value="KAI7747456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CVX1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01433">
        <property type="entry name" value="Ribosomal_L16_L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.10">
        <property type="entry name" value="Ribosomal protein L10e/L16"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047873">
        <property type="entry name" value="Ribosomal_uL16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016180">
        <property type="entry name" value="Ribosomal_uL16_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001197">
        <property type="entry name" value="Ribosomal_uL16_euk_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036920">
        <property type="entry name" value="Ribosomal_uL16_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11726">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00252">
        <property type="entry name" value="Ribosomal_L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005590">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54686">
        <property type="entry name" value="Ribosomal protein L16p/L10e"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7747456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="171" mass="19961" checksum="45E1EBF3DE1C6375" modified="2024-05-29" version="1" fragment="single">PARCYRQIKNKPYPKSRFCRGVPDPKIRIYDVGMKKKNADELPFCVHLTGMRGAFGKPLGTCARVAIGQVLLSVRCKDHHSHHAQEALRRAKFKFPGRQKIIVSRKWYEALRRAKFKFPGRQKIIVSRKWGFTKFSREDYIRWKKENRILLGRHGPLANREPGRAFLTSTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CXA0</accession>
    <name>A0AAD5CXA0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_002599</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006248">
        <property type="protein sequence ID" value="KAI7749941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CXA0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33566">
        <property type="entry name" value="EN/SPM-LIKE TRANSPOSON-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33566:SF1">
        <property type="entry name" value="EN_SPM-LIKE TRANSPOSON-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="567"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7749941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="567" mass="64404" checksum="7EF1A7F794C3F07E" modified="2024-05-29" version="1" fragment="single">VEIFEPKMRSTNIKRLKCKLKDIYFPYIQCDDLSKKGRTVMPIEFEVNGDDLAEIPGGEVATTNLASCNGPEFVLQIRFKLNHGNATTTSSQGTRSSNEANARLRCVYLPVKEGKESTESVIESLKESGCDYIEEFGSYKHVSCRRLGRLLPDARWAWLPFMDFRQRKTDRSQVFLKKSCMRVKCFIETDAGFSPTPSKTDFAHQNPFTTALRNLGSNQSLEKDSGVDIDIRRDGKSLNMTQLDKQYQLWLREMHERYDEEDNCGIDEPVLIVNPLNKKALHISRSSDVVRVHKAFRRNGKLWKSGQKIKILKGACAGFHSHNVYATLECILLEGFKEDAGGDGWMICRTIDISEKDGCLLDSIEDNPKFDLRKSVSIPINVIDSGKCVAVDDSEWKSQIEKQYQKCPSSIDILNSRQCRELGIVSSLPDGPETYANDVSPSEIVAVVRPATYKSGTPYKHLDQKYMMKDNFEMLLAITYSTNKKPRDEKNIYSGRVVPTSLKDFNGLYVFKPKCKSHPLFQKAGIYTFTFSIRDSSCEKRVVKVQVNASRDVCRWALAKKVSHHNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CYC8</accession>
    <name>A0AAD5CYC8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">RNA polymerase sigma factor sigE, chloroplastic/mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_012957</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006500">
        <property type="protein sequence ID" value="KAI7748595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CYC8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071482">
        <property type="term" value="P:cellular response to light stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1740.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000943">
        <property type="entry name" value="RNA_pol_sigma70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007624">
        <property type="entry name" value="RNA_pol_sigma70_r3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007630">
        <property type="entry name" value="RNA_pol_sigma70_r4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050239">
        <property type="entry name" value="Sigma-70_RNA_pol_init_factors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30603">
        <property type="entry name" value="RNA POLYMERASE SIGMA FACTOR RPO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30603:SF4">
        <property type="entry name" value="RNA POLYMERASE SIGMA FACTOR SIGE, CHLOROPLASTIC_MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04539">
        <property type="entry name" value="Sigma70_r3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04545">
        <property type="entry name" value="Sigma70_r4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00046">
        <property type="entry name" value="SIGMA70FCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="Sigma2 domain of RNA polymerase sigma factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="Sigma3 and sigma4 domains of RNA polymerase sigma factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0731">Sigma factor</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="RNA polymerase sigma-70 region 2" evidence="8">
        <location>
            <begin position="288"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase sigma-70 region 3" evidence="7">
        <location>
            <begin position="373"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase sigma-70 region 4" evidence="9">
        <location>
            <begin position="454"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="48"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7748595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="519" mass="58926" checksum="D48669F905F3DC4F" modified="2024-05-29" version="1">MGVVTVSSSAAAAARTPFGLTTRFPSQSPNKRSLTVVVLAFKTDKPTNPTTTTLVTSKDSPNLSVVDHTSKQNPKKLRRSKKSSDRVQAFSTAEAPYCSLDLDYLEAAAELNRIYNLSPSPATIDSDTLVKKGRKKISDTCEKPETRTPVDIVRGQRRRSQRLTLENRIHLKNKKAVAPITSNHKRKHVKVINEDEKVNKLVKDYSPSTDLGSLDWKKMKIPPVLPSSEHTRLFKLLQTMKATLKVKEELWKVLEREPTDGELAEATNMSVSQLSKRIGLGQAARNKLIKHNLRLVLFVMNKYFQEFANGRNFQDLCQAGVKGLITAIDRFEPGRKLQLSTYALFWIRHAIIRSMTLSSFYKVPFGLESVKIEIQKAKKKLWFENMRPPTEEEIAKKAGISLERYRDVTKTSKPILSLNRKNSVTQEEFINTFADDDGDERRQPALLRLALDDVLDSLKPKESLVIRQRYGLDGKGNRTLGEIAGNLNISREMVRKHEVKALMKLKHPARVEYLRHYLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-02-05" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CYX3</accession>
    <name>A0AAD5CYX3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_025566</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006140">
        <property type="protein sequence ID" value="KAI7750524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016125">
        <property type="term" value="P:sterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002403">
        <property type="entry name" value="Cyt_P450_E_grp-IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24286">
        <property type="entry name" value="CYTOCHROME P450 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24286:SF286">
        <property type="entry name" value="CYTOCHROME P450-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00465">
        <property type="entry name" value="EP450IV"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7750524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="404" mass="46396" checksum="96B190FD2A4A2647" modified="2024-05-29" version="1" fragment="single">QNGQQRLPPGSMGWPYIGETLSLYTQNPNTFFSMRQMRYGNIFKSHILGXXXXQSHLFKPTYPSTKEILIGPQAVFFHQGSYHSHVKKLIQSSFLPSAIKGQVHKIESIVLGLLASWEHVDTINTLHEMKKYSYDVAMLSVFGKEVEDNEIEGLKHLYHCIEKGYNSMPLNLPGTPFKKAMKARKVLNERLRKMIQKRKETGECEGGLLGVFLGLKENDQLSDSQIADNIIGVIFAAHDTTASVLTWLLKYLHDHPHLMNAVKIEQEEIWRERLVTKRGITWEDTRRMPLTSRVIQETLRVASILSFLFREAVEDVEIEGFLIPRGWKVLPLFRTIHYSPEFFPEPNKFDPSRFEMAPRANTYMPFGIGAHSWEVIGEGNGIEYVPFPVPKQGLPIKLHRIREV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CYY0</accession>
    <name>A0AAD5CYY0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SHSP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_006281</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006344">
        <property type="protein sequence ID" value="KAI7749539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CYY0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd06472">
        <property type="entry name" value="ACD_ScHsp26_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.790:FF:000009">
        <property type="entry name" value="17.6 kDa class I heat shock protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031107">
        <property type="entry name" value="Small_HSP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11527">
        <property type="entry name" value="HEAT-SHOCK PROTEIN 20 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="SHSP" evidence="6">
        <location>
            <begin position="46"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7749539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="160" mass="18133" checksum="99F8B6B420E39EA4" modified="2024-05-29" version="1">MSIIPSFFGRRRSNVFDPFSLDVWDPFEGFPFNNNNFGPLSSQSSETSSFVNANIDWKETSDAHVFKADVPGLKKEEVKVEVEDDRVLQISGERNKESEEKGDTWHRVERSSGKFVRRFRLPENAKVDQVKAAMENGVLTVTVPKVEVKKPDVKSIQISG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CZK8</accession>
    <name>A0AAD5CZK8_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_023774</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Phosphate:H(+) symporter (TC 2.A.1.9) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005946">
        <property type="protein sequence ID" value="KAI7751246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CZK8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23702">
        <property type="entry name" value="eL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050814">
        <property type="entry name" value="Myo-inositol_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020">
        <property type="entry name" value="PROTON MYO-INOSITOL COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020:SF12">
        <property type="entry name" value="PROTON MYO-INOSITOL COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="172"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7751246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="253" mass="27983" checksum="738A1C0AAE6BBDAD" modified="2024-05-29" version="1">MRAAAGGGQNGMIGMHQAMQSALAGNPIGMKQDGSDAASGRYDDRGYIIAWRGIVDDGTTFCSTCNLWCIHSGFSNCADEHNVTRPEKLRTERVTIELTPAGIWVVRFVCFRTVSTSASRFHAICYAGGPLNPVTARYVNIGRIALVMYDKDYGNLVVIVDVVDENMLPGTWRWWLGVAVVPAIIQFLLMLLMPESPRWPYMKMAVMSHMLIQRNSEDGVASVLDGRFAPATHLAVAFLFWNSKPFPTISDYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D0E1</accession>
    <name>A0AAD5D0E1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_028436</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006012">
        <property type="protein sequence ID" value="KAI7751037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D0E1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000009">
        <property type="entry name" value="PP2A_PR55"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11871">
        <property type="entry name" value="PROTEIN PHOSPHATASE PP2A REGULATORY SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00600">
        <property type="entry name" value="PP2APR55"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7751037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="95" mass="10810" checksum="5FAB5BE1B25AD359" modified="2024-05-29" version="1" fragment="single">MTLNHLFSFAEVITCAEFHPTHCNTLAYSSSKGSIRLIDLRQSALCDKHSKLFEEQEAPGTRSFFTEIIASISDIKFGRDGRYILSRDYMTLKVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D199</accession>
    <name>A0AAD5D199_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 14">Squalene monooxygenase</fullName>
            <ecNumber evidence="5 14">1.14.14.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">M8C21_016014</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">AA19_3_7</strain>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the stereospecific oxidation of squalene to (S)-2,3-epoxysqualene, and is considered to be a rate-limiting enzyme in steroid biosynthesis.</text>
    </comment>
    <comment type="function">
        <text evidence="13">Component of the triterpene saponins (e.g. ginsenosides or panaxosides) and phytosterols biosynthetic pathways. Catalyzes the first oxygenation step in sterol biosynthesis and is suggested to be one of the rate-limiting enzymes in this pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>squalene + reduced [NADPH--hemoprotein reductase] + O2 = (S)-2,3-epoxysqualene + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25282"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15440"/>
            <dbReference type="ChEBI" id="CHEBI:15441"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="EC" id="1.14.14.17"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:25283"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 14">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Terpene metabolism; lanosterol biosynthesis; lanosterol from farnesyl diphosphate: step 2/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Membrane</location>
            <topology evidence="2 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the squalene monooxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.14.17" evidence="5 14"/>
    <dbReference type="EMBL" id="JAMZMK010005943">
        <property type="protein sequence ID" value="KAI7751252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D199"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004506">
        <property type="term" value="F:squalene monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009725">
        <property type="term" value="P:response to hormone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016126">
        <property type="term" value="P:sterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046246">
        <property type="term" value="P:terpene biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000074">
        <property type="entry name" value="Squalene monooxygenase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013698">
        <property type="entry name" value="Squalene_epoxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040125">
        <property type="entry name" value="Squalene_monox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10835">
        <property type="entry name" value="SQUALENE MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10835:SF30">
        <property type="entry name" value="SQUALENE MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13450">
        <property type="entry name" value="NAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08491">
        <property type="entry name" value="SE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0274">FAD</keyword>
    <keyword evidence="6 14" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="463"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="490"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="domain" description="Squalene epoxidase" evidence="15">
        <location>
            <begin position="218"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00051556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00056623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU367121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF08491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAI7751252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="535" mass="58568" checksum="DE8CDF4A27841602" modified="2024-05-29" version="1">MENTVDNSYIVATFYVSLLAFVVLSVLRHRTSNSVSHSRKIGYGNVLKNRSDSTRGELFRRPYGDADADFIIVGAGVAGAALAYTLGKEGCKIRVIERDLTEPDRIVGELLQPGGYLKLIELGLEDCVEEIDAQRIHGYVLFKDGKNTKLSYPLEKFHSDVSGRAFHNGRFIQRMREKAATFPNVELEQGTVTSLLEDEGTIRGVQYKTKSGEALKAFAPLTIVCDGCFSNLRRSLCKPQVDVPSCFVGLALKNCELPYPNHGHVVLADPSPILFYPISSSEIRCLVDIPGQKLPSLSNGDMSRYLKTIVAPQVPCEVHAAFMAAIESGDIRTMPNRIMPASSVPTPGALLLGDAFNMRHPLTGGGMTVALADIVVLRDLLKPLHNFSDADSLTKYLESFYTLRKPMASTINTLAGALYMVFCASPDQARKEMQEACFDYLSLGGACSTGPVGLLSGLNPSPVCLVLHFFAVAVYGFGRLMLPLPTPKRIWLGVRLILGASWIIFPIIKAEGMMQMFFPARVPAYHRAHSKKGSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-10-08" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D2U6</accession>
    <name>A0AAD5D2U6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Methyltransferase</fullName>
            <ecNumber evidence="11">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_023488</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Endomembrane system</location>
            <topology evidence="10">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 11">Membrane</location>
            <topology evidence="1 11">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the methyltransferase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="11"/>
    <dbReference type="EMBL" id="JAMZMK010005860">
        <property type="protein sequence ID" value="KAI7751654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000119">
        <property type="entry name" value="probable pectin methyltransferase QUA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004159">
        <property type="entry name" value="Put_SAM_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10108:SF1139">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10108">
        <property type="entry name" value="SAM-DEPENDENT METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03141">
        <property type="entry name" value="Methyltransf_29"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 11" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU366043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7751654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="551" mass="62312" checksum="7BBC7B00286A0C68" modified="2024-05-29" version="1">MDVRIIPWLHKLQLFSGHRPPRNWLLLCVVSVFVLIALLSSSSSGGFTSFTDTVKPEVYTNYRRLKEHTASDYIELENVSSGSSEVKGIRLCGKEREDYVPCYNVSANVLAGYKDGEEFDRHCEVSQDEVFCLVRPPKDYKTPLSWPVGRDVIWNANVKISKDQFLSSGSMTKRLMLLEENQISFHSDDELIFDGVKDHSRQVAEMIGLGSDAEFWQAGVRTVLDIGCGFGSFGAHLLSLKLMAICMAAYELTGSQVQLSLERGLPAIIGNFISRKLPFPSLSYDMIHCAQCGVSWDQKDGMFLIEANRILKPGGYFVLHGTSLSTGKGSMIEEFTHKICWTLIGRQEETFIWXXXXNATHLGVQQDEFFEDFESSRSALRNYWSLLTPLIFSDHPKRPGDEDPLPPYNMIRNVMDMNARFGGLNSVFLEAGKSAWVMNVVPFRARNTLPLILDQGFIGVLHDWCEPFPTYPRTYDMLHANGLLSYLISEGCSITNLLLEMDRILRPEGWVVLSDKLGHIEDARVVATQIRWEARVIDLENDSDQRLKLVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D3I5</accession>
    <name>A0AAD5D3I5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Conserved oligomeric Golgi complex subunit 8</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Component of oligomeric Golgi complex 8</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_003363</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the COG8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005390">
        <property type="protein sequence ID" value="KAI7753571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D3I5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017119">
        <property type="term" value="C:Golgi transport complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007255">
        <property type="entry name" value="COG8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016159">
        <property type="entry name" value="Cullin_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21311">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX COMPONENT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21311:SF0">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04124">
        <property type="entry name" value="Dor1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74788">
        <property type="entry name" value="Cullin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="370"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="370"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="391"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="407"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7753571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="420" mass="46288" checksum="E423D0CDE0E6B642" modified="2024-05-29" version="1">MTELLPLASVSQQPYVSELLSFTLDRLHKEPELLRVDADRIRRQMQEVAVNNYRAFISAADALVSIRNEVSSIDNHLESLIAEVPKLTSGCSEFIDSAENILEKRKMNQTLLANHSTLLDLLEIPQLMDTCVRNGNYDEALDLEAFVSKLSTMHPKLQVIQALAAEVRQTTQSLLSQLLQKLRSNIQGCPEPIFQEYEYGSGEFSGEIVILLVLDSHRWVPLPAVGFSSNSFGEEGQEDVTPPPNLMEHPPLAVFINGVSAAMNELRPCAPLSLKHILAQELVKGLQTVSDSLLRYNTSRVLRESESVLFLSLCRAFIEVAYPHCATCFGRCYPGGSSLITDGNNLFEGLTRLLTTSSSKQLPKRVQNAATKNVSENGNSTAVENGGAVADVKETENSKVVEEDNADNTNSITQENPSEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D4J9</accession>
    <name>A0AAD5D4J9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_024322</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005580">
        <property type="protein sequence ID" value="KAI7752932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D4J9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026749">
        <property type="entry name" value="Tmem135"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12459:SF15">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 135"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12459">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 135-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7752932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="240" mass="27253" checksum="31DAD5D1A54E67EA" modified="2024-05-29" version="1">MLLTGFNTQHTSLAVYILMRAAVLASRCGIKSKRFGHVCKPLTWAHGDIFLMCLASTQILCAYILKQDSLPQSYKSFLNKHGGKDPIILQGVRDIACGMPFTNLEQIQKFYKNMGVDVILDPQMKVPCSIIHANQSCRWHAFSFFFQAYKRAVPVYLPVYLIPALIVHRQGLGKRIWTCFLFRIFKRCNVTMLALGTFPTGLSLAIEKKSRRIEISLYCLARAIESFFTTMSDIASQRQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D7B1</accession>
    <name>A0AAD5D7B1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_000458</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010003750">
        <property type="protein sequence ID" value="KAI7754410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D7B1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025322">
        <property type="entry name" value="PADRE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33413">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33413:SF33">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14009">
        <property type="entry name" value="PADRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7754410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="139" mass="15361" checksum="EB7F3C345288CFDD" modified="2024-05-29" version="1">MGNCQAIDNASLIIQHPTGKVEKMYSTISAAEVMKLNPSHYVALLLTTTVRHVATTKETNHGHVKANPQPVRVTRIKLLRHTESLTVGHIYRLVTTQEVMKGLMAKKNGKINNNIRQPKPSEQPAGCAKLTLSTTTWVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D7D6</accession>
    <name>A0AAD5D7D6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_007436</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005436">
        <property type="protein sequence ID" value="KAI7753441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D7D6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7753441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="63" mass="7327" checksum="D4FBAE09A4C514EC" modified="2024-05-29" version="1" fragment="single">SSLLFSSLQFKNDQLNVVFSRRQKQILTNGSLCEQTQVDIHNLVQSELKKNRRQNDLLDPLDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D871</accession>
    <name>A0AAD5D871_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_018806</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010002455">
        <property type="protein sequence ID" value="KAI7754807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D871"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015112">
        <property type="term" value="F:nitrate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010167">
        <property type="term" value="P:response to nitrate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010916">
        <property type="entry name" value="TonB_box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016605">
        <property type="entry name" value="Transptr_NO3_Nar2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34806:SF1">
        <property type="entry name" value="HIGH-AFFINITY NITRATE TRANSPORTER 3.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34806">
        <property type="entry name" value="HIGH-AFFINITY NITRATE TRANSPORTER 3.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16974">
        <property type="entry name" value="NAR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00430">
        <property type="entry name" value="TONB_DEPENDENT_REC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042276807" evidence="2">
        <location>
            <begin position="24"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7754807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="134" mass="14710" checksum="52EF9DB0BAABEA0E" modified="2024-05-29" version="1">MEGRARILVVSLLLSCFAVTSYAVYFSSLKETLVVTASPIKGQVLKAGEDKITVTWRFNQTYSAGTDSDYKTVKVAYGQTTDAHKSTNLFDIQAITGRHVSLDIASACFSAFSIVSLAGFFYMEKRKANTSQQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D9S7</accession>
    <name>A0AAD5D9S7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_021054</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000179">
        <property type="protein sequence ID" value="KAI7757118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D9S7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080043">
        <property type="term" value="F:quercetin 3-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080044">
        <property type="term" value="F:quercetin 7-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000040">
        <property type="entry name" value="UDP-glycosyltransferase 76C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926:SF1561">
        <property type="entry name" value="CYTOKININ 7-BETA-GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926">
        <property type="entry name" value="GLUCOSYL/GLUCURONOSYL TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7757118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="429" mass="47879" checksum="72BFDC062CD3AD0C" modified="2024-05-29" version="1">MSNNNTTTTDTKGRCLVLLPLPFQGHLSPMLQLANILHSKGFSITILHTSFNSPNPNNYPHFNYVPIPDTSEANLCVSSDDVGKVIRFMHDVNINLVDPIRGCLERLMSEDKGVACLITDAQWSCTQSVAEGLKLPRIVHRTSNISSFRSFAAYPASHDKGCVQSISDEETKSKALVPGLESLKVEALPKFHAYDAEVVSKVIERMVQATKRAHAYFATSSSSLLEQDKTSLSWLNKYPPKSVLYVSFGSIVKVDESQFLEIAWGLANSKQPFLWVVRPRSIEGSEWLEPLPNGFLDRIVEGRGYIVKWAPQQDVLAHPAIGGFWTHNGWNSTLESICEGVPMICSPSFGDQIPNARHVSNVWKIGVELEHRFKRREIESAIKKVMVDDEGLQLRNKIIDLKQIASFCLKKGGSSYSSLEDLVNYILSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D9Y1</accession>
    <name>A0AAD5D9Y1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="3">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_003480</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the peptidase C12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010000963">
        <property type="protein sequence ID" value="KAI7755640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D9Y1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.532.10">
        <property type="entry name" value="Peptidase C12, ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001578">
        <property type="entry name" value="Peptidase_C12_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036959">
        <property type="entry name" value="Peptidase_C12_UCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10589">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10589:SF17">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01088">
        <property type="entry name" value="Peptidase_C12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52048">
        <property type="entry name" value="UCH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="18"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="112"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="UCH catalytic" evidence="10">
        <location>
            <begin position="25"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS52048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7755640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="132" mass="14909" checksum="4505AD8F72A19EC9" modified="2024-05-29" version="1" fragment="single">DSHKQVVAIKTVDEIKNAQLACLFYIFVLSAYTATKHEFLWGLGLPPGEAECNDVYGLDEELLEMVPKPVLAVLFLYPITPESEQERLKQDCTKKDPCDGVYFMRQTSLQTAGAWCILNAAGCFFVLCLWHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DAE1</accession>
    <name>A0AAD5DAE1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">15.7 kDa heat shock protein, peroxisomal</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_013493</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000742">
        <property type="protein sequence ID" value="KAI7755865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DAE1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007052">
        <property type="entry name" value="CS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031107">
        <property type="entry name" value="Small_HSP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11527">
        <property type="entry name" value="HEAT-SHOCK PROTEIN 20 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51203">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="SHSP" evidence="4">
        <location>
            <begin position="17"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="CS" evidence="5">
        <location>
            <begin position="22"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7755865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="140" mass="15708" checksum="60B0A6661EAF48B0" modified="2024-05-29" version="1">MALFGDPFRRFFINPPMYRTGTATGLMDWYETPQAHFIKINVPGYNKEEVKVQVEEGNVLVVKAEAAKETSKEKEKETVWHVAERGGGLNGFSREIELPEDVKVDQIKAQVDSGVLTVVLPKDLSPKPSKVRNIHVSSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DBD9</accession>
    <name>A0AAD5DBD9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HVA22-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_032834</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000479">
        <property type="protein sequence ID" value="KAI7756289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DBD9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004345">
        <property type="entry name" value="TB2_DP1_HVA22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12300">
        <property type="entry name" value="HVA22-LIKE PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12300:SF117">
        <property type="entry name" value="LP05237P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03134">
        <property type="entry name" value="TB2_DP1_HVA22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="80"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="114"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="151"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU362006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7756289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="216" mass="24173" checksum="AAE502D5D3C54B8B" modified="2024-05-29" version="1">MYSEAKLAFYIYLWYPKTMGTTYVYDSFFRPYISKHEPEIDRNLMELKTRAGDAFVLYWQRAASYGQTSIFDVLQYIASQSTPRPRPPQRPQTGRPRPAVPVTQQETNEPPSPVSSSSSDSASHQQETADAEHRPQVPPTPSSPAAVLRAQKSTSSQSLTVAEKPQTSNEAEMMETEETGSSNPDPNPPVKEVVMEKPVKVYTRARSRKGSVSSVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DC11</accession>
    <name>A0AAD5DC11_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Photolyase/cryptochrome alpha/beta domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_004424</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000206">
        <property type="protein sequence ID" value="KAI7756989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DC11"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036155">
        <property type="entry name" value="Crypto/Photolyase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006050">
        <property type="entry name" value="DNA_photolyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47832">
        <property type="entry name" value="DNA PHOTOLYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47832:SF1">
        <property type="entry name" value="DNA PHOTOLYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00875">
        <property type="entry name" value="DNA_photolyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52425">
        <property type="entry name" value="Cryptochrome/photolyase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51645">
        <property type="entry name" value="PHR_CRY_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Photolyase/cryptochrome alpha/beta" evidence="1">
        <location>
            <begin position="48"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7756989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="676" mass="75838" checksum="276AC12D5E0BD1D0" modified="2024-05-29" version="1">MAILLFPHTPTLTYHRHSISLTPVRWSSPATTVRFTTSNAKKNTTTQDTALLWYKNDLRVDDHPGLIAASKHKALLPLYVFDHRILRDFNEEMFELILYALKDLRDSLKDLGSNLMIRFGRTESVIQDLVKEVRAANVYTQEEVEYDSRLVIDKVKETLGDVFLEKDCPKISLWGSSFYDLKNMMDIPLSYDEFQRLKLDVHLPLSAPNFPSVVPDLAWGPLPTLNDLKEFMDESPYKLKDSWSLIKNTSAAYVLQNDRILMSDGGTNIQGSLNSLGSQITKKKLKKSASITKQGNFVGGGTSDVLDALAAYLKYFEGTARDDWQELHERLRNAETREGASFGFLFGPVLQLGVVSGRRVYNEAIKYEKDRNGGFLSPFGYSCFTVASSADHVCSREYAVMGGEGPAILLVHGFGAFLEHYRDNITNIVDGGNRVWAITLLGFGRSEKPNVVYTELMWAELLRSFIVDVVGEPVHLVGNSFGGYFVSIVAGLWPALARSVVLVNSAGHYIPGYSSVPPSKERKTTGIAWLGARGLSFYLRLSFRNLVKRCYPTKTDRADERLLNEMSYDPGVLVVLESIFTFDPSIPLNYLLKGFMRKVLIIQGMKDPISDSKTKVNVVKKHFKEIAIKELDAGHCPHDELPEEVNSIIREWVVNIESRPGILEQTASDGNRIAAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DCT9</accession>
    <name>A0AAD5DCT9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_031669</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010001074">
        <property type="protein sequence ID" value="KAI7755495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DCT9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045283">
        <property type="entry name" value="AT3G44326-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33736:SF12">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33736">
        <property type="entry name" value="F-BOX PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="354"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="6"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7755495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="377" mass="42709" checksum="3EE2C7492171A2F8" modified="2024-05-29" version="1">MSVGSAENMANLSSDLFYDILKRLDGATLASASCACAAFSSMSKEERLWQNVCSSMWPSTKRDDVKSLISQIGGFKKFYADCFPLIVNKNVSEFTWSDYPEYTEELTEAEYYGDFDEFQNVSPSDFVFIVDILYKDKTICSKVIWGIPNANGFNGWFSNCPFRIDLFSFSGQGDDHTGGVTLSVDDGLPLVTSMERERKDGKLWQELRDGIRLSWIVVNTKAKKAANLSSWSPLGGQRHWPTEKDFLLRFGSILPAEDILPCQMVECILAMKFQVTEASEVQTTLKLTELCMQLGDMEGAHVNGRNSLFVLNEALNCNRSKNYNLAVESCHLYSKVQSEIKEEKMRYESRLDKLWILGGITVFVTLCYYLCKIRCHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DEF4</accession>
    <name>A0AAD5DEF4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_013703</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000005">
        <property type="protein sequence ID" value="KAI7758342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DEF4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009658">
        <property type="term" value="P:chloroplast organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010027">
        <property type="term" value="P:thylakoid membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040299">
        <property type="entry name" value="RF2K-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34938">
        <property type="entry name" value="PROTEIN FERTILITY RESTORER RF2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34938:SF1">
        <property type="entry name" value="PROTEIN FERTILITY RESTORER RF2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7758342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="124" mass="13572" checksum="1FE028D1AA206F96" modified="2024-05-29" version="1" fragment="single">QKSSSLSLFPNPTWTISLFFSGRRRQQHRRKVVDRPKNIPNSIKGTLTLHLRSEVPKVGLCSLPLRLLTTQPTTFGGHVQISPVKSAVFICAAASNARMGAEQTQTVTRQSSTITIAPIQGIHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DHA3</accession>
    <name>A0AAD5DHA3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">1-deoxy-D-xylulose-5-phosphate synthase</fullName>
            <ecNumber evidence="6">2.2.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_003176</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the transketolase family. DXPS subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.7" evidence="6"/>
    <dbReference type="EMBL" id="JAMZMK010000087">
        <property type="protein sequence ID" value="KAI7757670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DHA3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008661">
        <property type="term" value="F:1-deoxy-D-xylulose-5-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019288">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02007">
        <property type="entry name" value="TPP_DXS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07033">
        <property type="entry name" value="TPP_PYR_DXS_TK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.920:FF:000002">
        <property type="entry name" value="1-deoxy-D-xylulose-5-phosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000005">
        <property type="entry name" value="1-deoxy-D-xylulose-5-phosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00315">
        <property type="entry name" value="DXP_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005477">
        <property type="entry name" value="Dxylulose-5-P_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033248">
        <property type="entry name" value="Transketolase_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003933">
        <property type="entry name" value="PRK05444.2-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43322">
        <property type="entry name" value="1-D-DEOXYXYLULOSE 5-PHOSPHATE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43322:SF3">
        <property type="entry name" value="1-DEOXY-D-XYLULOSE-5-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13292">
        <property type="entry name" value="DXP_synthase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02780">
        <property type="entry name" value="Transketolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="TK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword evidence="11" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Transketolase-like pyrimidine-binding" evidence="13">
        <location>
            <begin position="441"/>
            <end position="608"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7757670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="763" mass="83213" checksum="21ECB68980498D46" modified="2024-05-29" version="1">MMCTASAHCSLGIVTAHVHQTSRNFVSKLESSSSLIFRSGGESVGIGLSFSSGVSVCSKGCVGRLCALPDISDIFPEKLPTPILDTIESPMHLKNLSHKELKQLADDIRSELSFIRNETQKSVKGSMSVVELTIAIHHVFSAPMDKILWDAGEQTYAHKILTGRRSVMHTLRQKNGISGYTSRSESEFDPFGAGHGCNSISAGLGMAIARDIKGKRERVVAVISNESIMSGQLYEAMSNAGYLDSDMVVILNDSRQSLHSKLEDAPKTPINALSSAFSKLQSSRLFRKFREAAKGLTKKIGKGMYEWAAKVDEYARGMIGPPGSTLFEELGLYYIGPVDGHNIEDLICVLNQVASLDSTGPVLVHVITTENKETREEKHIGISKKYNQGLGIEIGMGIPRDGMEWVITTLMMRDRYHEILPIYVEPEDPYDSDPQPSNRTQTYSDWFGEALTMEAQLDKDIMVVLAGSGMGMDPSLKLFRDKFPDRLFDVGMAEQHAVTFSAGLSCGGLKPFCIIPSTFLQRAYDQVVHDVDRQKIPVRFVIPSAGLVGSDGPTNAGAFDITFMSCLPNMIVMAPSDEVELANMVATASCIDDRPVCFRYPRGAIVKSNTSVCHGVPIEIGKGRVLVEGKDIALLGYGSMVQNCLRAQSLLSELGIQVTVADARFCKPLDIKLVRQLCENHSFLITVEEGSIGGFGSHVAQFITLDGKLDGSIKWRPIVLPDNYVEQASPMEQLALAGLTGHHIAATALNLLGRTREALLLMC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G193</accession>
    <name>A0AAD5G193_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_030449</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012187">
        <property type="protein sequence ID" value="KAI7724570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G193"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7724570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="71" mass="8111" checksum="CAC039E250F4DE24" modified="2024-05-29" version="1" fragment="single">PLLFSSSSPFFSTTSITYYFSPSIRSPSRIDGVEGGARERWRRSLKLGFRLDGQTPAAKNSRIRMKIYVEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G275</accession>
    <name>A0AAD5G275_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_024775</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011844">
        <property type="protein sequence ID" value="KAI7725845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G275"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7725845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="80" mass="8871" checksum="198ECB28CE3C69E2" modified="2024-05-29" version="1">MKLFTTMLRYAGTGVELLQSEICRHWSCSRTILSATGWSNDEYEKLYCINADENLVGALCTELPGGKSGGKRGRRILVSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G2E3</accession>
    <name>A0AAD5G2E3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_008010</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011702">
        <property type="protein sequence ID" value="KAI7726354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G2E3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044647">
        <property type="entry name" value="RTNLB17/18/21"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46626">
        <property type="entry name" value="RETICULON-LIKE PROTEIN B17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46626:SF2">
        <property type="entry name" value="RETICULON-LIKE PROTEIN B17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041909679" description="Secreted protein" evidence="1">
        <location>
            <begin position="24"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7726354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="127" mass="14170" checksum="24123B4978A30774" modified="2024-05-29" version="1">MLSLISQLGLLCLGVSFFSNMIAQRNGVRTKREVTLKEDVLRAARVVLPAINFAISKARELFSREPSMTLKGSLSASLAQNCIRCTLTKYARKVKMQKHGYWKHGSLLLCPVKKLALKSSDTTDQSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G3B5</accession>
    <name>A0AAD5G3B5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">chitinase</fullName>
            <ecNumber evidence="4">3.2.1.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">M8C21_021772</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">AA19_3_7</strain>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Defense against chitin-containing fungal pathogens.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1-&gt;4)-beta-linkages in chitin and chitodextrins.</text>
            <dbReference type="EC" id="3.2.1.14"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyl hydrolase 19 family. Chitinase class I subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.14" evidence="4"/>
    <dbReference type="EMBL" id="JAMZMK010011762">
        <property type="protein sequence ID" value="KAI7726071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G3B5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008843">
        <property type="term" value="F:endochitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016998">
        <property type="term" value="P:cell wall macromolecule catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00325">
        <property type="entry name" value="chitinase_GH19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00035">
        <property type="entry name" value="ChtBD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.10:FF:000003">
        <property type="entry name" value="Class IV chitinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.20.10:FF:000001">
        <property type="entry name" value="Endochitinase (Chitinase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.530.10:FF:000052">
        <property type="entry name" value="Endochitinase PR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.530.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.20.10">
        <property type="entry name" value="Endochitinase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.10">
        <property type="entry name" value="Endochitinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001002">
        <property type="entry name" value="Chitin-bd_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036861">
        <property type="entry name" value="Endochitinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016283">
        <property type="entry name" value="Glyco_hydro_19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000726">
        <property type="entry name" value="Glyco_hydro_19_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22595">
        <property type="entry name" value="CHITINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22595:SF193">
        <property type="entry name" value="ENDOCHITINASE EP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00182">
        <property type="entry name" value="Glyco_hydro_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001060">
        <property type="entry name" value="Endochitinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00270">
        <property type="entry name" value="ChtBD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="Lysozyme-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57016">
        <property type="entry name" value="Plant lectins/antimicrobial peptides"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50941">
        <property type="entry name" value="CHIT_BIND_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00773">
        <property type="entry name" value="CHITINASE_19_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00774">
        <property type="entry name" value="CHITINASE_19_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="9" id="KW-0146">Chitin degradation</keyword>
    <keyword evidence="5 16" id="KW-0147">Chitin-binding</keyword>
    <keyword evidence="10 15 16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0611">Plant defense</keyword>
    <keyword evidence="13" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 17" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042224882" description="chitinase" evidence="17">
        <location>
            <begin position="23"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-1" evidence="18">
        <location>
            <begin position="22"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="14">
        <location>
            <position position="130"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15 16">
        <location>
            <begin position="27"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15 16">
        <location>
            <begin position="32"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="86"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="148"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="234"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR001060-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR001060-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAI7726071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="266" mass="28833" checksum="C41788C722316368" modified="2024-05-29" version="1">MKTLGILIVANILLAGILNSNAQNCGCSPDLCCSRFDFCGSDEAYCGAGCQEGPCFSAPPTNNVDVASIVTDAFFNGIVDQSDASCEGRGFYTRAAFLEALGNYPQFGRVGSEEDSRREIAAFFAHLTHETGHFCYIEEINGPSRDYCDENNTQYPCNPSKGYYGRGPIQLSWNYNYGPAGRSIGFDGINNPEIVATDPVISFRTALWFWMNNVHSIIGQGFGATIRAINGMECDGGNAATVTARVQYYSQYCNQLGVAPGNNLRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G4E7</accession>
    <name>A0AAD5G4E7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">(S)-2-hydroxy-acid oxidase</fullName>
            <ecNumber evidence="3">1.1.3.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_001828</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.3.15" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010011234">
        <property type="protein sequence ID" value="KAI7728350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003973">
        <property type="term" value="F:(S)-2-hydroxy-acid oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02809">
        <property type="entry name" value="alpha_hydroxyacid_oxid_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000056">
        <property type="entry name" value="hydroxyacid oxidase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012133">
        <property type="entry name" value="Alpha-hydoxy_acid_DH_FMN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000262">
        <property type="entry name" value="FMN-dep_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037396">
        <property type="entry name" value="FMN_HAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008259">
        <property type="entry name" value="FMN_hydac_DH_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10578:SF67">
        <property type="entry name" value="PEROXISOMAL (S)-2-HYDROXYACID OXIDASE GLO3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10578">
        <property type="entry name" value="S -2-HYDROXY-ACID OXIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01070">
        <property type="entry name" value="FMN_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000138">
        <property type="entry name" value="Al-hdrx_acd_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00557">
        <property type="entry name" value="FMN_HYDROXY_ACID_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51349">
        <property type="entry name" value="FMN_HYDROXY_ACID_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0288">FMN</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FMN hydroxy acid dehydrogenase" evidence="9">
        <location>
            <begin position="1"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="271"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="269"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="271"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="274"/>
        </location>
        <ligand>
            <name>glyoxylate</name>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="302"/>
            <end position="306"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="325"/>
            <end position="326"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000138-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000138-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7728350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="382" mass="42538" checksum="FCDFC539A4D4D65A" modified="2024-05-29" version="1">MGDEPVNVKEYQQLAKQALPKMYYDFFAGGSEDQHTLRENVEAFQRITLRPRILVDVSKIDMSTTILGYKTSAPIMIAPTSMHKLAHPQEHDPFNXXXXSSTCTIEEVASSCNAVRFFQLYVYKRREISALMVKRAELNGFKAILLTVDSPKLGRREADIKNKMIAPQLKNLEGLVSTEVHDASEITIFLLVYWVLYMHITLMIITSDLQSGGSNLEAFASRTMDPSLSWKDIAWLKSITKLPILIKGVLTCEDVIKAMEIGVEGIIVSNHGARQLDYVPATIDALEEVVLTVQGRIPVLFDGGIRRGTDVFKALALGAQAVMVGRPIVYGLAAKGEHGVKRVIEMLKDELELTMALSGCPTLNDITRNHVRTQIDGRHSRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G4H1</accession>
    <name>A0AAD5G4H1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_007358</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011297">
        <property type="protein sequence ID" value="KAI7727917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G4H1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019748">
        <property type="term" value="P:secondary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033124">
        <property type="entry name" value="Ser_caboxypep_his_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF382">
        <property type="entry name" value="PEPTIDASE S10, SERINE CARBOXYPEPTIDASE, ALPHA_BETA HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00560">
        <property type="entry name" value="CARBOXYPEPT_SER_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7727917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="288" mass="33098" checksum="75C475BECBA09A5A" modified="2024-05-29" version="1">MITEELYKGNDEGLEPIVNIQGYIAGNPLTDKTGDLNSRLEYAYRLAFISQELYEATKKDCKENYANANSDNVQCMLDLYEVDKRVKGINIQQILDPDCKPETHLFKSGNPVAKSNPIKMLPTLGDTFCRDELYDYATVWANDENVMKSLNVREGTVKEWLLCNLDMKYNYSNPFMPQYEFNVQSSVVYHEMLTKRNCRALIFSGDHDMMVSHVGTLKWINSLNLTITENNWDAYYVDRQASGFTTSYAHHNYSLVFATVKGAGHTAPEFKPAECFALVRRWFARRPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G4J0</accession>
    <name>A0AAD5G4J0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_027573</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011403">
        <property type="protein sequence ID" value="KAI7727301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G4J0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15315:SF35">
        <property type="entry name" value="F21J9.10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15315">
        <property type="entry name" value="RING FINGER PROTEIN 41, 151"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7727301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="247" mass="28556" checksum="BFE99D0FD2E2757D" modified="2024-05-29" version="1">MEMMYYPLGRSSYEDSLKVIEADIQHANALAAAIPRAKDGARFQMKLVHDQLTPLIMFLLQWIDSSCACLLPRYLNLFHVIIYKVYTDGRPKISRHGRKATVNDFYAVILPSLRRLHYDLVELDNGPKEESPKLQISSSKKLEKDEKFTNIDLEREDECGICLEPCTKIVLPNCCHAMNSRSASCPFCRGNIKRVKSRDLWVLTCNDEVIDADLVSKEDLVRFYLYINNLPKDSPDALFFMFYEYLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G4J1</accession>
    <name>A0AAD5G4J1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">O-acyltransferase WSD1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_016547</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011200">
        <property type="protein sequence ID" value="KAI7728500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G4J1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008374">
        <property type="term" value="F:O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019432">
        <property type="term" value="P:triglyceride biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045034">
        <property type="entry name" value="O-acyltransferase_WSD1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009721">
        <property type="entry name" value="O-acyltransferase_WSD1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31650:SF52">
        <property type="entry name" value="DIACYLGLYCEROL O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31650">
        <property type="entry name" value="O-ACYLTRANSFERASE (WSD1-LIKE) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06974">
        <property type="entry name" value="WS_DGAT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="O-acyltransferase WSD1 C-terminal" evidence="1">
        <location>
            <begin position="62"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7728500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="171" mass="19515" checksum="1C25AF7EF7E4D13C" modified="2024-05-29" version="1">MHTINDVLFGIVSYGLSKYLDSRSSKSIREGLRMTGVAMVNLRPQREYQDLEEFVRDPKAGWGNKFGMLVLPTYYHRSGSDPLEYLRRAKAMIDQKKSSLESYFSYKFGYLIMSILGAKAITMHMVSYAGTAYMQILVAKDLVHDPEVLAKCLEDAMLEMKEAALPKIKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G6U1</accession>
    <name>A0AAD5G6U1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_015749</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010948">
        <property type="protein sequence ID" value="KAI7729656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G6U1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34553:SF5">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34553">
        <property type="entry name" value="OS05G0597400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="136"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="177"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="273"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="306"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="331"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="442"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="451"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7729656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="482" mass="55510" checksum="93AE8BFEEF3E004D" modified="2024-05-29" version="1">MLGDEFHDASSEFPDSHSTNNNENISSACVSEPNGSPCTPRCNLGEKIETPLNETSILHTPPPDGPYKRRKLMSPIGIDINSSFYTEITHRETVCTPVVSSYAYDCEETTEPMEYKDALLMFRFNDRDLPFELRKIILFDIRLLTLLEAGLPSWVIFCQSYPVFCHIYRPWMCPLARALYVAISIVTVVIGFYDLYKNVPVLKATASRLCGPLFDWIETWEMVSRIKYLGTMLFLHNSEKAVMWFLMMTRTIRSFVSIITQPLRGPFSDFIEVVLPVWTLLIQTGQYLFSFVWVLMENSWDVLEDLIEVVLMPVWFISSVIWIIVSTVMYPILWCLWGALYAPVSLILGLSDFVGYVYNQTYDLARDTWLFASGVFKLASNAESAVNTYEVSIWRSLWNDLFSQVFRAVRSILNGFVAFFAACNRHRLSIYNHLQEFIRTGDQPAHRPMPMNSSHRSQGSRSQTREEAGERQTPPRRNLKQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G7P4</accession>
    <name>A0AAD5G7P4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Lipoxygenase</fullName>
            <ecNumber evidence="12">1.13.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">M8C21_022303</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">AA19_3_7</strain>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Plant lipoxygenase may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding.</text>
    </comment>
    <comment type="pathway">
        <text evidence="12">Lipid metabolism; oxylipin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 12">Belongs to the lipoxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.13.11.-" evidence="12"/>
    <dbReference type="EMBL" id="JAMZMK010010433">
        <property type="protein sequence ID" value="KAI7731547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G7P4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016702">
        <property type="term" value="F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034440">
        <property type="term" value="P:lipid oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031408">
        <property type="term" value="P:oxylipin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.245.10:FF:000002">
        <property type="entry name" value="Lipoxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.375.10">
        <property type="entry name" value="Lipoxygenase-1, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.372.10">
        <property type="entry name" value="Lipoxygenase-1, Domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.245.10">
        <property type="entry name" value="Lipoxygenase-1, Domain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.60.20">
        <property type="entry name" value="PLAT/LH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000907">
        <property type="entry name" value="LipOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013819">
        <property type="entry name" value="LipOase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036226">
        <property type="entry name" value="LipOase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020834">
        <property type="entry name" value="LipOase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001246">
        <property type="entry name" value="LipOase_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027433">
        <property type="entry name" value="Lipoxygenase_dom_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001024">
        <property type="entry name" value="PLAT/LH2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036392">
        <property type="entry name" value="PLAT/LH2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11771">
        <property type="entry name" value="LIPOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00305">
        <property type="entry name" value="Lipoxygenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01477">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00087">
        <property type="entry name" value="LIPOXYGENASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00468">
        <property type="entry name" value="PLTLPOXGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00308">
        <property type="entry name" value="LH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49723">
        <property type="entry name" value="Lipase/lipooxygenase domain (PLAT/LH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48484">
        <property type="entry name" value="Lipoxigenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00081">
        <property type="entry name" value="LIPOXYGENASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51393">
        <property type="entry name" value="LIPOXYGENASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50095">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="10 12" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="2 12" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4 12" id="KW-0925">Oxylipin biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PLAT" evidence="13">
        <location>
            <begin position="95"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="Lipoxygenase" evidence="14">
        <location>
            <begin position="213"/>
            <end position="859"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAI7731547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="859" mass="96787" checksum="E3175D2076F71179" modified="2024-05-29" version="1">MLKAQINQSYPLHKLLPLHNKPFIRGGPTNYSSFSSYSPTTSTRKSNVTCVRSTSTAAGNIKAVAAPVVTTATTVKSVITIKPVILSALTANANNNNNVDAAAADGDSDLLALSILLEFVSNDLDANGNRKSVMVTATYKGLDIVNRVYKYEGNFDIPEDFGEIGAVYVENQLKRKMFFNTIALDNGVTFTCESWVQSKYDVKDKRIFFTDKSYLPSETPEALKSLRTEDLESLRGNGEGERKSYDRIYDYDVYNDLGDPDLDLSLARPVLGGKEYPLPRRCRTGRPMCTKDPLTESRTTDSFYVPCDEDFSQIKEIYFNARTLFALLHGVIPTLKAIFTDKDKGFPLFTSINKLYNEGVNIPPPDKDVEGALPRLIKKAIDAANAILKFETPETMDRDIFSWFRDEEFCRQMLAGLNPCSIQLVTEWPLMSKLDPKVYGPPESAITKEIVQEEIGGDMTFDEALAQKKLFMLDYHDLLLPYHVYTPTWDATDAWLWKLAKAHVVSHDSGYHQLVSHWLRAHGVTEPYIIATNRHLSQMHPIKRLLEPHFRYTMQINALARYSLINAGGIIESTFSPEITCMQLASDAYDQLWRFDNQALPADLISRGMAVEDQSAPHSLKLTIKDYPFANDGLLIYDAIKEWATSYVNYYYPQANLVESDEELQAWWNEIRTVGHADKKDEPWWPQLKSQDDLIGILSTIIWVASGHHSAVNFGQYDFAGYFPNRPSIARTKMPNEDPTDEEWQAFMKRPEDALLKCFPSQVQAVQVMAVLDVLSGHSPDEEYIGETIEPAWEAEPAIKAAFEEFQGKLNQLESIIDSRNNDPVLKNRNGAGLVPYELLKPYSDRGVTGRGVPNSISI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G9M6</accession>
    <name>A0AAD5G9M6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_011814</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009735">
        <property type="protein sequence ID" value="KAI7734410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G9M6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7734410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="122" mass="13876" checksum="53BDB6E2270FCD7F" modified="2024-05-29" version="1" fragment="single">DGVNVDVTDNASLFAITDDSVFQLCDRKRKDRGKFYFDYGGAPTIQALISDRKFLSMWTTSYDFYAFLDQKSLLIVVEYIQCDGTLIRCAQIKKIGVQIIQNALMVPHVSCTNYLLIAIFED</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GAY0</accession>
    <name>A0AAD5GAY0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Chlorophyll A-B binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_027750</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009898">
        <property type="protein sequence ID" value="KAI7733841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GAY0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053091">
        <property type="entry name" value="PSII_Assembly/Photoprotect-Rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37752">
        <property type="entry name" value="OS02G0610700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37752:SF1">
        <property type="entry name" value="OS02G0610700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103511">
        <property type="entry name" value="Chlorophyll a-b binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7733841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="144" mass="15473" checksum="5DE9BBB772679E7F" modified="2024-05-29" version="1">MICCSSSLSTANGGFLLHQPSSSSFSSNPHSATPIRWGVKRGIGSGINRTKGQVANANVTIASGKGKKEVIMVDPVEAKRLAAKQMEDIKRKQTFKRRRQIEAINGAWAMLGLTAGIVIEGQTGNGILAQLAGYWEVVVNFFVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GC49</accession>
    <name>A0AAD5GC49_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Small ribosomal subunit protein uS4 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_000258</name>
        <name evidence="4" type="ORF">M8C21_012642</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009194">
        <property type="protein sequence ID" value="KAI7736767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAMZMK010006361">
        <property type="protein sequence ID" value="KAI7749438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GC49"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001912">
        <property type="entry name" value="Ribosomal_uS4_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00163">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01390">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS4 N-terminal" evidence="3">
        <location>
            <begin position="3"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7736767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7749438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="81" mass="9927" checksum="0DFA357F6E0A2EA1" modified="2024-05-29" version="1">MRKLKFHEKKLLKKVNFLEWKREGGHREAHVMHRYHVTGRDDYKKYSSLCRMAQKLVNILKQMDPRDPYRIQMTDALLEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GCN2</accession>
    <name>A0AAD5GCN2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TPX2 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_011929</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TPX2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009514">
        <property type="protein sequence ID" value="KAI7735283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GCN2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027329">
        <property type="entry name" value="TPX2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044806">
        <property type="entry name" value="WVD2/WDL1-4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46372:SF26">
        <property type="entry name" value="(WILD MALAYSIAN BANANA) HYPOTHETICAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46372">
        <property type="entry name" value="PROTEIN WVD2-LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06886">
        <property type="entry name" value="TPX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TPX2 C-terminal" evidence="7">
        <location>
            <begin position="178"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="112"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="178"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="232"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="278"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="312"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF06886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7735283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="357" mass="38497" checksum="0BC8ECE8239EB0A7" modified="2024-05-29" version="1" fragment="single">MESGNGVNGEDENGQEKSNEVESSTLEVSESCEDVNGSEISNSVEVSGNEGSSKNNEVAKNGPKGGPSDRKPKPKPKAKSRLTQSLSFPAKPRNNESMRSSMDGHPVKARATLSNASATNPANRRASTGMKTKESGPNVRMSRPKKPNGSEDGPPSAETLGVAPGGQHKNSVVGFSSRLEERAEKRKEIEEKINAKEAEKTNLQEKSKENQEAEIKQLRKTLMFKAAPMPKFYKEPPPKVDLKKIPTTRPKSPKLGRKKSNEAAATNGTVVSPHVTKNRTSSPDVNTTNGDKDAAAKRTIRKSASKALSSKTEVKSEKSKENPTQEEEKECIEQSNESASPNPLEAESASPNPLEVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GCU2</accession>
    <name>A0AAD5GCU2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_024714</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the PP2C family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="4"/>
    <dbReference type="EMBL" id="JAMZMK010008991">
        <property type="protein sequence ID" value="KAI7737590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GCU2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009788">
        <property type="term" value="P:negative regulation of abscisic acid-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000065">
        <property type="entry name" value="Protein phosphatase 2C 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000222">
        <property type="entry name" value="PP2C_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01032">
        <property type="entry name" value="PPM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 10" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="12">
        <location>
            <begin position="110"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="78"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="87"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7737590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="410" mass="44330" checksum="8A51FD152980C54A" modified="2024-05-29" version="1">MAGMCCGVVGETKTSSTVEPSGGGQNRRRHMEIQQLKFYAAAASNVVAPLQESSPKRRKVIETIASIKAKTIVQSSDVKQGGVGCGSSSSSSSLSSEDSMEEVLDKESSRFGLTVVCGRRRDLEDAVAVKPSFCKTNPPESDDLHFYGVYDGHGCSHVAMKCKDRMHEIVKEEVENCDKSSIEWKETMVNSFSRMDKDVTEWTSSASSSSSTCRCELQTPQCDAVGSTAVVAVVTPEKIVVSNCGDSRAVLCRNGVAIPLSTDHKPDRPDELQRIEEAGGRVIYWDGARVLGVLAMSRAIGDNYLKPYVIPEPEVTITERSDEDECIILASDGLWDVVSNEVACSVARMCLRSHEVPSPPRSPGSVLNVARGESSDKACSDASILLTKLALARRSTDNVSVLVVDLRRNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GF17</accession>
    <name>A0AAD5GF17_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">WRKY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_029627</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the WRKY group II-b family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008966">
        <property type="protein sequence ID" value="KAI7737728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GF17"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.80:FF:000002">
        <property type="entry name" value="probable WRKY transcription factor 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.80">
        <property type="entry name" value="WRKY domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003657">
        <property type="entry name" value="WRKY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036576">
        <property type="entry name" value="WRKY_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044810">
        <property type="entry name" value="WRKY_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31429">
        <property type="entry name" value="WRKY TRANSCRIPTION FACTOR 36-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31429:SF54">
        <property type="entry name" value="WRKY TRANSCRIPTION FACTOR 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03106">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00774">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118290">
        <property type="entry name" value="WRKY DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50811">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="WRKY" evidence="8">
        <location>
            <begin position="254"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="17"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="153"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="493"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="26"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="40"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="53"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="502"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00061007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7737728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="524" mass="58111" checksum="9356F998979DB8A1" modified="2024-05-29" version="1">MAREEGVDSDMEIDLTLKLLDAQDQEPAKDSSKSDHVDDHVDDQDESNDDDVGDTKVEAHEHEVQELENDLQTTNNSTEALESQDLNTHHQEKLSMLQKEMNRMKEENKVLRQVVEKTMKDYYDLQMKFSIIQQSSNQIKDPRSFLSLNVHDGNQETKKSSPRSPPLQESENEDLGLSLRIQSNTTSQYARDSHEGNHKELLENATRFAPMQQSNLINSGNFSGDNNIKAVNIVNDQMASLPNRKARVSVRARCEAPTMNDGCQWRKYGQKIAKGNPCPRAYYRCTVAPGCPVRKQVQRCLEDMSILITTYEGNHNHPLPVGATAMASTTSAAAGSFMLLDSKNPLSSELGAMNPSPLTNYYMSSGNINHDSSPYSSTYARTMNSNDPSKGIVFDLTNNHINPHHIPISNLSPQLGFPWMSNKFPNGSHVSRSKQVDMFNGPIHEGDEVNNNNNNNNSVLVENMSAIASHPNFRVAVAAAISSIINKESQITNNIHADGPSDRASGGSSSDGKTWVLESLSRRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GFV3</accession>
    <name>A0AAD5GFV3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">peroxidase</fullName>
            <ecNumber evidence="4">1.11.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">M8C21_026572</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">AA19_3_7</strain>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 a phenolic donor + H2O2 = 2 a phenolic radical donor + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:56136"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:139520"/>
            <dbReference type="ChEBI" id="CHEBI:139521"/>
            <dbReference type="EC" id="1.11.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="14">Binds 2 calcium ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
        <text evidence="14">Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peroxidase family. Ascorbate peroxidase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.7" evidence="4"/>
    <dbReference type="EMBL" id="JAMZMK010008716">
        <property type="protein sequence ID" value="KAI7738821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GFV3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140825">
        <property type="term" value="F:lactoperoxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.420.10:FF:000006">
        <property type="entry name" value="Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.420.10">
        <property type="entry name" value="Peroxidase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002016">
        <property type="entry name" value="Haem_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000823">
        <property type="entry name" value="Peroxidase_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019793">
        <property type="entry name" value="Peroxidases_heam-ligand_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31388:SF215">
        <property type="entry name" value="PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31388">
        <property type="entry name" value="PEROXIDASE 72-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00141">
        <property type="entry name" value="peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00458">
        <property type="entry name" value="PEROXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00435">
        <property type="entry name" value="PEROXIDASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50873">
        <property type="entry name" value="PEROXIDASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0106">Calcium</keyword>
    <keyword evidence="11 15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0349">Heme</keyword>
    <keyword evidence="10 14" id="KW-0408">Iron</keyword>
    <keyword evidence="7 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="16" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042108591" description="peroxidase" evidence="16">
        <location>
            <begin position="25"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="Plant heme peroxidase family profile" evidence="17">
        <location>
            <begin position="34"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="14">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="119"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="18">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAI7738821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="240" mass="25937" checksum="C6AF957DC87DCA0C" modified="2024-05-29" version="1" fragment="single">SSRTKLVTIFIILISLNYFMPCNAQLSTTFYDTTCPNALSTIRTSIDAAVSNNRWWTVMDSKRLGRRDSTGSNAQQAGTDLPRGNFNLGQLISSFAGKGLNEREMVALSGSHTLGQARCIRFRERIYSSSLRIDAAFNSSLSAVCPPTPPNGDGNMQPLDLVTPNAFDNNYFSNLVSSRGLLISDQVLFNGDSADSIVTEYVDNPATFASDFAAAMVKMSEIEVLTGSSGIIRTLCTSAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GG25</accession>
    <name>A0AAD5GG25_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">MBD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_030955</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008739">
        <property type="protein sequence ID" value="KAI7738733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GG25"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00122">
        <property type="entry name" value="MBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.890.10">
        <property type="entry name" value="Methyl-cpg-binding Protein 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038945">
        <property type="entry name" value="MBD13-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001739">
        <property type="entry name" value="Methyl_CpG_DNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34067">
        <property type="entry name" value="OS04G0193200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34067:SF20">
        <property type="entry name" value="OS08G0206700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01429">
        <property type="entry name" value="MBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50982">
        <property type="entry name" value="MBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MBD" evidence="7">
        <location>
            <begin position="21"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="84"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="126"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="198"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="85"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="247"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="268"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="437"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="468"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="481"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="506"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="546"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="723"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7738733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="723" mass="78131" checksum="850DB33E6869AEA7" modified="2024-05-29" version="1" fragment="single">MSPVKSPEEFGSRNVGKRDKIVVEKDEAEELPPGWTKEVRMRKTGSATKVDRYYTDPVTGYIFHSLKDVQRYLRTGEVGRLATKPKIEEKEKPSELEEQMSETLTNKRAPAAYKCELPARASKRQAGINGDVALSSPPPSQAKTRQAIQMAGVKVDAPSLSRSLPESKATRLAEVAIDASPSPPEPKTRQATRLAGVAVDASVPSPPEPKTRQATRLAGVVIDVSPSLPEPKTRQATRLAEVAIDASRSNPEPKTRQATRPAEVPVDASPPSPLPPEPKTRQATRLAGVMVDASPSLPVPKTRQATRLAEVATDASLPSPSLPEPKTRQATRLAGVAVDESLPSASAPDTRSNHQARKQTGIKADELVPSPPPAVPKTRQAARQAGIEIDSLPQLPRDSKIPQATPLKGTEVGPSPPSQPEPKTRQAKRAAGFRDNASPSRPKTRRATQQAGIEIISPPPSLPDARTGRQAARTRQVRSTTLKENDKNTEAEKITDKHNGNADMDANTSHNQENSIITPPSNQESNEKQEPRNILPPVTPKEPNKQIVTGPTTSQLPEVQPPPMAVPPVNLDKQESSVVYPTMSNNPLPQDSYSYGSDMKSDSSINFSMNDLWTDPCIEFAVKTLTGAIPFTDVNKVENFTAPSEVPLEALWTDPCIEFAVKTLTGAIPVPEDNFLLHRPASSSNPQGPHNFSSPMNVCQTGHKNKQQDLVFPTFGNGGLQKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GG79</accession>
    <name>A0AAD5GG79_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_021610</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008223">
        <property type="protein sequence ID" value="KAI7741282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GG79"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7741282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="62" mass="6892" checksum="2CBA42BE7E1FCDFA" modified="2024-05-29" version="1">MVGQLLMTAGVVEEIATIKTVVMYRNLGLVVMYLNLFIGDSHSVYLEQPGLEVKKLTRKHSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GGE6</accession>
    <name>A0AAD5GGE6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_005222</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008679">
        <property type="protein sequence ID" value="KAI7739063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GGE6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052222">
        <property type="entry name" value="DESIGUAL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31769">
        <property type="entry name" value="OS07G0462200 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="83"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7739063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="102" mass="10922" checksum="E23B4E4CEEAAC036" modified="2024-05-29" version="1">MEVTTKNMALVVGTLGVASFILGVIAENKKPASGTPITGKDVVICKYPADPTVALGYLSFGFLFLSTLAGGFSLFYPYKGKSIPWPALFQSTSFFIFFLIAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GGI1</accession>
    <name>A0AAD5GGI1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_009543</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008614">
        <property type="protein sequence ID" value="KAI7739496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GGI1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7739496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="123" mass="13568" checksum="3130EEA388107AEC" modified="2024-05-29" version="1">MEVARKGEDSGYGVPCLLISAKNDLNLYPMAIKDSEMAVKINAPICISAKEKYMNNVFQKIVTTAQKCHLSVPETEHGRNKKRYRQLVNRSLVLASVGAAIAIVALAAYRAYACRKVKSPRQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GMV3</accession>
    <name>A0AAD5GMV3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_000339</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">May modulate chromatin structure by regulation of nucleosome assembly/disassembly. Could function together with B-type cyclins in the regulation of microtubule dynamics.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the nucleosome assembly protein (NAP) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006484">
        <property type="protein sequence ID" value="KAI7748690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GMV3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1500:FF:000001">
        <property type="entry name" value="Nucleosome assembly protein 1-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1120.90:FF:000005">
        <property type="entry name" value="Nucleosome assembly protein11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1500">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1120.90">
        <property type="entry name" value="Nucleosome assembly protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037231">
        <property type="entry name" value="NAP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002164">
        <property type="entry name" value="NAP_family"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11875">
        <property type="entry name" value="TESTIS-SPECIFIC Y-ENCODED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00956">
        <property type="entry name" value="NAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143113">
        <property type="entry name" value="NAP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="60"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00056035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7748690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="357" mass="40590" checksum="1ECB6D721C6935EE" modified="2024-05-29" version="1">MSTNPEESQFNMSDLTSNLPPSTTGLSAEDRAGLVNVLKSKLNDLTGQHPDFLDSLSVNVRQRVALLKDLQSQHDELEAKYLEEKAQLEAKYLKLYEPLYAKRYEIVNGLTEVEGVSNDNTGDKENNESARGVPNFWLTAMKSNEVLADEISESDEGALQYLKDIKWCRLDGAKGFKLELYFNPNPYFKNSVLTKTYEMINEEEHILEKAIGTEIEWFPGKNLTQKVLRKKPKKGLKSIKPPITKTENCESFFNFFNPPQIPDDEEELDEDTAELLQNQMEHDYDIGSTIRDKIIPHAVSWFTVKHLSTTEFKIGIQLRLVILMWFNSFTGENGMGSRSGSVPSALIENPTLHTYVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GN24</accession>
    <name>A0AAD5GN24_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">ATP synthase subunit beta</fullName>
            <ecNumber evidence="18">7.1.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">M8C21_009928</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">AA19_3_7</strain>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.</text>
    </comment>
    <comment type="function">
        <text evidence="18">Produces ATP from ADP in the presence of a proton gradient across the membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17 18">
            <text>ATP + H2O + 4 H(+)(in) = ADP + phosphate + 5 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:57720"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.1.2.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="18">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) and CF(0) have multiple subunits.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a, b and c.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.1.2.2" evidence="18"/>
    <dbReference type="EMBL" id="JAMZMK010006417">
        <property type="protein sequence ID" value="KAI7749022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GN24"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042776">
        <property type="term" value="P:proton motive force-driven mitochondrial ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18110">
        <property type="entry name" value="ATP-synt_F1_beta_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18115">
        <property type="entry name" value="ATP-synt_F1_beta_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01133">
        <property type="entry name" value="F1-ATPase_beta_CD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1140.10:FF:000001">
        <property type="entry name" value="ATP synthase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.170:FF:000006">
        <property type="entry name" value="ATP synthase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000026">
        <property type="entry name" value="ATP synthase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.910">
        <property type="entry name" value="ATP synthase, F1 beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1140.10">
        <property type="entry name" value="Bovine Mitochondrial F1-atpase, Atp Synthase Beta Chain, Chain D, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01347">
        <property type="entry name" value="ATP_synth_beta_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055190">
        <property type="entry name" value="ATP-synt_VA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042079">
        <property type="entry name" value="ATP_synt_F1_beta_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020971">
        <property type="entry name" value="ATP_synth_F1_beta_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005722">
        <property type="entry name" value="ATP_synth_F1_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050053">
        <property type="entry name" value="ATPase_alpha/beta_chains"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036121">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024034">
        <property type="entry name" value="ATPase_F1/V1_b/a_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01039">
        <property type="entry name" value="atpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15184">
        <property type="entry name" value="ATP SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15184:SF80">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT BETA-1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22919">
        <property type="entry name" value="ATP-synt_VA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11421">
        <property type="entry name" value="Synthase_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039072">
        <property type="entry name" value="ATPase_subunit_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47917">
        <property type="entry name" value="C-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50615">
        <property type="entry name" value="N-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16 18" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="9 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="15 18" id="KW-0139">CF(1)</keyword>
    <keyword evidence="7" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="6 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="11" id="KW-1278">Translocase</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="19">
        <location>
            <begin position="214"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU003553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAI7749022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="548" mass="58935" checksum="F85F9FCC94209A59" modified="2024-05-29" version="1">MASRRLLSSLIRRVKPPSITKTNPPIQTPRFRPSPLGFLLNRASGFATAAAVAPASPSSSNATGKITDEHSGTGAVGQVCQVIGAVVDVRFEDGLPPILTALEVMDNPTRLVLEVAQHMGENVVRTIAMDGTEGLVRGQKVLNTGSPILVPVGRPTLGRIINVIGEAIDHRGELKTDHYLPIHREAPPFVEQATEQQILVTGIKVVDLLAPYQRGGKIGLFGGAGVGKTVLIMELINNIAKAHGGFSVFAGVGERTREGNDLYREMIESGVIKLGDQQSESKCALVYGQMNEPPGARARVGLTGLTVAEHFRDAEGQDVLLFIDNIFRFTQANSEVSALLGRIPSAVGYQPTLATDLGGLQERITTTKKGSITSVQAIYVPADDLTDPAPATTFAHLDATTVLSRQISELGIYPAVDPLDSTSRMLSPRILGEEHYKTARGVQEVLQNYKNLQDIIAILGMDELSEDDKLTVSRARKIQRFLSQPFHVAEVFTGAPGKYVELKESVTSFQGVLDGKYDDLPEQSFYMVGGIDEVIAKAEKIAKENAGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GN72</accession>
    <name>A0AAD5GN72_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">M8C21_007294</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">AA19_3_7</strain>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010007093">
        <property type="protein sequence ID" value="KAI7745873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GN72"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000004">
        <property type="entry name" value="Calcium-dependent protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000086">
        <property type="entry name" value="calcium-dependent protein kinase SK5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="28"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="17">
        <location>
            <begin position="177"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="17">
        <location>
            <begin position="213"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="17">
        <location>
            <begin position="249"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="17">
        <location>
            <begin position="285"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAI7745873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="346" mass="39223" checksum="6CC71A67575ADDE8" modified="2024-05-29" version="1">MKKHTKNSSLKPSNTVLSHATPRLRDHFIMGKKLGHGQFGTTYLCRQKSTGVDFACKSIPKRKLLCKEDYDDVWREIQIMHHLSEFPCVVRIKGAYEDNVFVHLVMELCAGGELFDRIIQKGHYSEKEAAKLMKTIVGVLGNFSSTSHTPFVLPNIFCITAHELLDQIVIAERLSEEEIGGLKQLFKMLDTDNSGTITFEELKQGLKKVGSNLTESEIKELMDAADIDSNGTIEYGEFLAATLHMNKLEREENLLAAFRFFDKDGSGYITRDELQQASKDFGLQDAQLDEMIREVDQDNDGRIDYAEFTAMMRKGDDEVRSRAMKGNLNFDLAEALSFNKNKESQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GP74</accession>
    <name>A0AAD5GP74_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_026289</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006189">
        <property type="protein sequence ID" value="KAI7750192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GP74"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7750192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="72" mass="7844" checksum="746F22F2C2F812A8" modified="2024-05-29" version="1" fragment="single">GINGGGSGRIPIIVGGSAKGQVVVGLWWLISSEQGSRVRVLVSDDGGTVEFDRRHDEDTLGFTLDLHQPRWW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GPE9</accession>
    <name>A0AAD5GPE9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_029039</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006707">
        <property type="protein sequence ID" value="KAI7747701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GPE9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044274">
        <property type="entry name" value="RFI2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46798:SF18">
        <property type="entry name" value="CHROMATIN REGULATOR PHD FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46798">
        <property type="entry name" value="OS09G0511500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7747701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="280" mass="31658" checksum="1B2B4DFF8EB0FD4C" modified="2024-05-29" version="1">MQCPNCRKVESGRWLFADGSAHAVSETGVQDWMPHEGPHDFSYSRRPIGLRWHPFGGFMVHSSFEETESLTLNTFAHFLGNYAMIAEHTAALSPARSYVSHFRPNEHVANSNIQHPLNSHPAPSNNIRSTYIQHPSWGWSCHFLPYNADRDFIDHGNLTPVNPTTLRSTQAQAQAQPIYYTQRLVSRSRESSGANSQSRERNQIHHAAYHHEQPGEVTNMPSSTYGFRRFGGALALPMILPAVPHQPHNHGVHGSYISETERDESSYVPIIHGSFHHRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GRR1</accession>
    <name>A0AAD5GRR1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein ApaG</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_026920</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006056">
        <property type="protein sequence ID" value="KAI7750939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GRR1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1470">
        <property type="entry name" value="ApaG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050718">
        <property type="entry name" value="ApaG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007474">
        <property type="entry name" value="ApaG_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036767">
        <property type="entry name" value="ApaG_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001943">
        <property type="entry name" value="UVR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036876">
        <property type="entry name" value="UVR_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003967">
        <property type="entry name" value="PRK05461.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47191">
        <property type="entry name" value="OS05G0170800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47191:SF2">
        <property type="entry name" value="OS05G0170800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04379">
        <property type="entry name" value="DUF525"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02151">
        <property type="entry name" value="UVR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110069">
        <property type="entry name" value="ApaG-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46600">
        <property type="entry name" value="C-terminal UvrC-binding domain of UvrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51087">
        <property type="entry name" value="APAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50151">
        <property type="entry name" value="UVR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UVR" evidence="2">
        <location>
            <begin position="71"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="UVR" evidence="2">
        <location>
            <begin position="106"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="ApaG" evidence="3">
        <location>
            <begin position="149"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="76"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7750939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="279" mass="31874" checksum="92DFDB74C4A2CE0D" modified="2024-05-29" version="1">MMHSVSLKLYAEFNNLTSLHTTPTRFTFGPLNFPVRLNRSKRNCKLIARVSERDDGGTESEQNTTSFYERNQMYVLMKQQMELAALSENYEEAARLRDSLKLFEEEEPVLRLQGLMKEAIANERFEDAARYQNQIKEIAPHYLLKCSSDATTLGIRVQAKSVYIEGRSQPLRGQYFFAYRIRITNNSNRPVQLLKRHWIVTNANEKSEDVWGIGVIGEQPVLLPNDSFEYSSACPLTTPSGRMEGDYEMKYIDKVGSKTFSVSIAPFALSTLGDVTDNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GSN0</accession>
    <name>A0AAD5GSN0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_019389</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006119">
        <property type="protein sequence ID" value="KAI7750598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GSN0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34380">
        <property type="entry name" value="BNAA03G12380D PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34380:SF1">
        <property type="entry name" value="OS01G0221300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="190"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="217"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="14"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="190"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="249"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="280"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7750598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="463" mass="52002" checksum="7C3D28E25A8EA532" modified="2024-05-29" version="1">MKKMAVETDFDKVFENFRKRCLELEEKSKTAELRCLILETEVENLKKKNEELEGKIAAVQAVVIDPNYVKDDSETLLEVMIENKVLECEKRKAESDVCFWKEKVKELELKMKGMNERVEGEVDVGSRGGKDVFHRKRLSFEEGECPNKLGPSTPGVGQSTFHGVIDTNDGDDNTNEISKVKNLRKCDGLTKSQCSSENTTNLEHPDEEHTDEFAANLSNTRSAKRKRAANIVTSDDEDSYDDNAPICTLKTQHNSQVSTDSEEEVKDKSSIDLFGTFSSSEDEEYNDGIDESESEGGSLDGFIVDSSGSLSNCDSESSDSTDVSEDALNEYKATLDMIGRKKISNMKWDLEGDMLSDFGKDPALCMSAVCAVYRQQTADEQASKETIYRNKRGFSQADAWRASKLADFLMDGDSNGDLKKTVEELKQYDSEGIKLCRKLATTYSNQLFQIYQNKEDPYFPPQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GTX4</accession>
    <name>A0AAD5GTX4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cornichon</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_015907</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cornichon family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010001117">
        <property type="protein sequence ID" value="KAI7755442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GTX4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003377">
        <property type="entry name" value="Cornichon"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12290">
        <property type="entry name" value="CORNICHON-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03311">
        <property type="entry name" value="Cornichon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01398">
        <property type="entry name" value="Cornichon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="97"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7755442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="122" mass="14647" checksum="AA74FC957A92D512" modified="2024-05-29" version="1">MVFDLIAWILSFFILLAVMALVLYQIMCFLDLEIDYINPYELATKINNITLPEFITQGALCFLHLVARHWIMYIHRRHLVYATEVFSELSREKKQRIFKLVYLAVLLFFSLFWTTVEPYKFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GW62</accession>
    <name>A0AAD5GW62_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_004304</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010001220">
        <property type="protein sequence ID" value="KAI7755364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GW62"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12725">
        <property type="entry name" value="HALOACID DEHALOGENASE-LIKE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12725:SF118">
        <property type="entry name" value="PYRIDOXAL PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7755364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="118" mass="13887" checksum="0E6268A36C4491B3" modified="2024-05-29" version="1" fragment="single">MEYENEYQQQISQSRFECLLFDVDDTLYPFSSGLSAECTKNIKEYMVNKLDIEESKVPEMCAQLYKDYGTTMAGLRAIGYEFDHDDYHSFVHGRLPYEYLKPDHVLRNLLVSLPIRKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GX31</accession>
    <name>A0AAD5GX31_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_000891</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000513">
        <property type="protein sequence ID" value="KAI7756219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GX31"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045296">
        <property type="term" value="F:cadherin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033371">
        <property type="entry name" value="ARGLU1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31711">
        <property type="entry name" value="ARGININE AND GLUTAMATE-RICH PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31711:SF2">
        <property type="entry name" value="ARGININE_GLUTAMATE-RICH 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="79"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7756219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="112" mass="13196" checksum="B89D41847CD2A9EA" modified="2024-05-29" version="1" fragment="single">MPRSISRSPTSYRRRYSPSPVSRRRRRDRSRSPYTTTHNSRRRSRSGTPRRRRSRSPVSRHKKSRSPTPRRHRRRRSRTASVSPAKSPSPSTTSAERNNALAKSIKEMEEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GXJ5</accession>
    <name>A0AAD5GXJ5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_004872</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010001314">
        <property type="protein sequence ID" value="KAI7755293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GXJ5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14703">
        <property type="entry name" value="bZIP_plant_RF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044797">
        <property type="entry name" value="At4g06598-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044759">
        <property type="entry name" value="bZIP_RF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46835:SF3">
        <property type="entry name" value="BASIC-LEUCINE ZIPPER (BZIP) TRANSCRIPTION FACTOR FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46835">
        <property type="entry name" value="BASIC-LEUCINE ZIPPER (BZIP) TRANSCRIPTION FACTOR FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BZIP" evidence="3">
        <location>
            <begin position="223"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="171"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="316"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="238"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="177"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="193"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="350"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7755293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="379" mass="42640" checksum="713CB5AADB4599A6" modified="2024-05-29" version="1">MDNSKRPSTMRSMMYNGKHSLLPPKSPFPSIAPSYADYVSNSGIGPKGPPKNRDGSPHHQRTSSESFLLEEQPSWLDDLLNEPETPVKRGHRRSSSDSFTYMEAANAAYNEYQAQTEYQLRNLSSVPSWGSQDFDIYKDFRNPSFYGDTNAMIKNRNRAWESPQNATKHNFAHHTSKSLGSSREVDGNTSIATEKKEAVESVRQDADTSERKDPSVIKGSTSETDTKRAKQQFAQRSRVRKLQYIAELEKNVNALQAEGSEVAAEVEFLNQQSLILGMENKALKQRLDNLAQEQLIKYMEHEVLEREIGRLRALYQQQQQPPPPPAHRRSASKDKLDAQFSNLSLKKDSGSTSDVSVGDSPEVVTIERDAAFHVLAYAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GZR3</accession>
    <name>A0AAD5GZR3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_004518</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000130">
        <property type="protein sequence ID" value="KAI7757378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GZR3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007623">
        <property type="term" value="P:circadian rhythm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039928">
        <property type="entry name" value="LNK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33334">
        <property type="entry name" value="PROTEIN LNK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33334:SF10">
        <property type="entry name" value="PROTEIN LNK4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7757378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="146" mass="16260" checksum="6CEB09C9CDAB33BF" modified="2024-05-29" version="1">MVYFCINSLLEVDPIKGSTGPHDNTNLGNNTNIFEGEMVNSQCDGSHGQSFGSSKYLKTHAFSPSTDCGNGEVSTTYQMLQQCTSNENMEESVLNDLERATAHFTDKTRICFRDAFYRLAENSKQSLNPCQDESMMTSIDDTIRCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-10-08" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BLG9</accession>
    <name>A0AAD5BLG9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_029672</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011975">
        <property type="protein sequence ID" value="KAI7725324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051762">
        <property type="term" value="P:sesquiterpene biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47955:SF13">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47955">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 71 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0349">Heme</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7725324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="464" mass="52743" checksum="725873F5E0842DA9" modified="2024-05-29" version="1">MEPSTILSIVVSLLVLVTCWVLLTGKTQKNMPPGPPKLPIIGNIHQLEKQTPHRNLRNLARKYGPIMHLQLGQVSTVVISTPKLAQEILKIQDLCFANRPAATTPQIFFYKATSIGWANYGSYLREIKKIATLELFSPLKVKSFSYIRETELTRVFKFLDSCCGTPINFREMTVELVNNIVSRATLGDVCKDRQYLVDSAFYMLKTFSAFNLFNYYPRLNFLNVITGKKAQWLKMHKELDIILQKLLEEHRSRPLSSADHEDLVDVLLRVKDTGDLEFPITDDNIKAIILEMLTAGTSSSSMTIEWAFCEMMRNPKIMKKAQSEVRTVVKGNAITEANIQSMHYMKLVIKETMRLHCVPILLPRLNLKDCVVNGYHIPAKTTILTVGKILTASFRXXXXRRMCPGVNFGLATVEYAIANLLLHYDWKLPNDIKPHDIDMREFTRISTLPIHPLQIVPISVSGPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BLP5</accession>
    <name>A0AAD5BLP5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">H(+)-exporting diphosphatase</fullName>
            <ecNumber evidence="2">7.1.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_014792</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.1.3.1" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010011894">
        <property type="protein sequence ID" value="KAI7725610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BLP5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009678">
        <property type="term" value="F:diphosphate hydrolysis-driven proton transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004427">
        <property type="term" value="F:inorganic diphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004131">
        <property type="entry name" value="PPase-energised_H-pump"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01104">
        <property type="entry name" value="V_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31998">
        <property type="entry name" value="K(+)-INSENSITIVE PYROPHOSPHATE-ENERGIZED PROTON PUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03030">
        <property type="entry name" value="H_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="14"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="93"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="132"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="180"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="322"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="363"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="400"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7725610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="458" mass="48781" checksum="B618411F24D9C296" modified="2024-05-29" version="1" fragment="single">MTSATILSDLGTEILIPVCAVVGILFSLFQWYLVAQVKLTGEKQGGEGKNGFNEALIEEEEGVNDHAVVQKCAEIQNAISEGATSFLFTEYQYVGVFMVIFAILIFVFLGSVEGFSTSSKVCTYDTTKVCKPALMTAVFSTVSFVLGAITSVVSGFLGMKIATYANARTTLEARKGVGKAFIVAFRSGAVMGFLLAANGLLVLYITINLFKLYYGDDWEGLFESITGYGLGGSSMALFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAESSCAALVVASISSFGNTHDFTAMMYPLTVSSVGIIVCLITTLFATDFFEVKTVKQIEPSLKNQLIISTVLMTIGIAIVSWIALPSSFTIFNFGTQKVTKNWQLFLCVCVGLWAGLIIGFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BMD2</accession>
    <name>A0AAD5BMD2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CTP synthase</fullName>
            <ecNumber evidence="9">6.3.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">UTP--ammonia ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_006637</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>UTP + L-glutamine + ATP + H2O = CTP + L-glutamate + ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:26426"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Pyrimidine metabolism; CTP biosynthesis via de novo pathway; CTP from UDP: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the CTP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.2" evidence="9"/>
    <dbReference type="EMBL" id="JAMZMK010011857">
        <property type="protein sequence ID" value="KAI7725774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BMD2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003883">
        <property type="term" value="F:CTP synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044210">
        <property type="term" value="P:'de novo' CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019856">
        <property type="term" value="P:pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004468">
        <property type="entry name" value="CTP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017456">
        <property type="entry name" value="CTP_synthase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11550">
        <property type="entry name" value="CTP SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11550:SF34">
        <property type="entry name" value="CTP SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06418">
        <property type="entry name" value="CTP_synth_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 9" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="3 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 9" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CTP synthase N-terminal" evidence="11">
        <location>
            <begin position="65"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase" evidence="10">
        <location>
            <begin position="247"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU810713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF06418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7725774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="365" mass="40857" checksum="17358ECDA34FABC4" modified="2024-05-29" version="1">MAVMLVTTCLTSLVIILCLHKPPMLALCFLLSFGSIDLLYFSASLVKFREGCINEDWVLRTASEHTNYHITFMVQVVPHITDAIQDWIERVAAIPVDGKEGPPDVCVIELGITIGDIESMSFIEAIGQFSYRVCAGNVCLIHVSLVSVLHVVGEQELDTNVKEKLSRFCHVPEENIVNLYDVSSIWHIPLLLREQKAHEEILKVLELPWCILIASNPVLGEWTARAQIRIAMVGKYIGLSDSYLYVLKSADGVLVTGGFGDRGVEGKIIAAKYARENNIPYLGICLGMQTAVIEYARSVLGLKDANSTKFDQDTKNPCVIFMPEVSKTHMGGTMRVGLTYGNQRFIAERHRHRYDVWLFSRVKEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BNW2</accession>
    <name>A0AAD5BNW2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Glycine cleavage system P protein</fullName>
            <ecNumber evidence="11">1.4.4.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_031768</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">The glycine cleavage system catalyzes the degradation of glycine. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO(2) is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 11">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[glycine-cleavage complex H protein] + glycine + H(+) = N(6)-[(R)-S(8)-aminomethyldihydrolipoyl]-L-lysyl-[glycine-cleavage complex H protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:24304"/>
            <dbReference type="Rhea" id="RHEA-COMP:10494"/>
            <dbReference type="Rhea" id="RHEA-COMP:10495"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83143"/>
            <dbReference type="EC" id="1.4.4.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10 11">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer. The glycine cleavage system is composed of four proteins: P, T, L and H.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 11">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the GcvP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.4.4.2" evidence="11"/>
    <dbReference type="EMBL" id="JAMZMK010011852">
        <property type="protein sequence ID" value="KAI7725811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BNW2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009941">
        <property type="term" value="C:chloroplast envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005960">
        <property type="term" value="C:glycine cleavage complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016594">
        <property type="term" value="F:glycine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004375">
        <property type="term" value="F:glycine dehydrogenase (decarboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019464">
        <property type="term" value="P:glycine decarboxylation via glycine cleavage system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00613">
        <property type="entry name" value="GDC-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000025">
        <property type="entry name" value="Glycine cleavage system P protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000005">
        <property type="entry name" value="Glycine dehydrogenase (decarboxylating), mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000007">
        <property type="entry name" value="Glycine dehydrogenase (decarboxylating), mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003437">
        <property type="entry name" value="GcvP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049316">
        <property type="entry name" value="GDC-P_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049315">
        <property type="entry name" value="GDC-P_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020581">
        <property type="entry name" value="GDC_P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00461">
        <property type="entry name" value="gcvP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11773:SF12">
        <property type="entry name" value="GLYCINE CLEAVAGE SYSTEM P PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11773">
        <property type="entry name" value="GLYCINE DEHYDROGENASE, DECARBOXYLATING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21478">
        <property type="entry name" value="GcvP2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02347">
        <property type="entry name" value="GDC-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5 10 11" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Glycine cleavage system P-protein N-terminal" evidence="12">
        <location>
            <begin position="80"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="domain" description="Glycine dehydrogenase C-terminal" evidence="13">
        <location>
            <begin position="812"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="10">
        <location>
            <position position="734"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="929"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR603437-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU364056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF21478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7725811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="929" mass="100949" checksum="CB896BE8B6F4E2E9" modified="2024-05-29" version="1" fragment="single">MERARRLANKAILGRLVSQTKHQSSSSPSLYSPSRYVSSLSPYICSGTNVRSDRNLHGFGSQVRSISVDALKPSDTFPRRHNSATPEEQTKMAEFVGYPHLDALIDATVPKSIRLDSMKYSKFDEGLTESQMIAHMQELASKNKLFKSFIGMGYYNTSVPTVIIRNIMENPGWYTQYTPYQAEIAQGRLESLLNFQTMVTDLTGLPMSNASLLDEGTAAAEAMAMCNNILKGKKKTFIIASNCHPQTIDICKTRADGFDLKVVTSDLKDFDYSSGDVCGVLVQYPGTEGEVLDYSEFIKNAHANGVKVVMASDLLALTLLKPPGELGADIVVGSAQRFGVPMGYGGPHAAFLATSQEYKRMMPGRIIGLSVDSSGKPALRMAMQTREQHIRRDKATSNICTAQALLANMAAMFGVYHGPEGLKTIAKRVHGLAGTFAAGLKKLGTVQVQDLPFFDTVKITCADSHAIAEEAYKHKMNLRIVDKNTITVSFDETTTVEDVDTLFKVFALGKPVTFTAASIAPEVQDAIPSGLVRETPYLTHPIFNMYHTEHELLRYLSKLQSKDLSLCHSMIPLGSCTMKLNATTEMMPVTWPAFADMHPFAPVEQAQGYQEMFKNLGDLLCSITGFDSFSLQPNAGAAGEYAGLMVIRAYHMARGDHHRNVCIIPVSAHGTNPASAAMCGMKIITVGTDSKGNINIEEVRKAAEANKENLSALMVGLTSPGFIGADVCHLNLHKTFCIPHGGGGPGMGPIGVKKHLAPYLPSHPVVATGGIPAPEKSQPLGTISAAPWGSALILPISYTYIAMMGSQGITDASKIAILNANYMAKRLENHYPILFRGVNGTVAHEFIVDLRPLKTTAGIEPEDVAKRLIDYGFHGPTMSWPVPGTLMIEPTESESKAELDRFCDALISIREEIAEIEKGKVDLNNNVIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BPJ8</accession>
    <name>A0AAD5BPJ8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tryptophan synthase</fullName>
            <ecNumber evidence="3">4.2.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_033501</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine = D-glyceraldehyde 3-phosphate + L-tryptophan + H2O</text>
            <dbReference type="Rhea" id="RHEA:10532"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:58866"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.2.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 5/5.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.20" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010011411">
        <property type="protein sequence ID" value="KAI7727202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BPJ8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009570">
        <property type="term" value="C:chloroplast stroma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004834">
        <property type="term" value="F:tryptophan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000004">
        <property type="entry name" value="Tryptophan synthase beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023026">
        <property type="entry name" value="Trp_synth_beta/beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48077:SF3">
        <property type="entry name" value="TRYPTOPHAN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48077">
        <property type="entry name" value="TRYPTOPHAN SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0822">Tryptophan biosynthesis</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7727202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="244" mass="26204" checksum="C60FADC2630B97FA" modified="2024-05-29" version="1">MALSSSSNITYRATSITKPASLTYPRSYLSQSSLTFNKVSTFRPKSLTVSCVMTQQPEAAAAQSTVLRPDSLGRFGKFGGKYVPETLMYALSELESAFHALATDAEFQAAGFGLDSGKHAATLTKGEVGVLHGAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSFLKDLGRAEYYSITDAEALEAFKRLSRLEGIIPALETSHALAYLEKLCPTLPNGTKVVLNCSGRGDKDVHTAIKHLQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BRF3</accession>
    <name>A0AAD5BRF3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_006628</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the major facilitator superfamily. Phosphate:H(+) symporter (TC 2.A.1.9) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011528">
        <property type="protein sequence ID" value="KAI7726829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BRF3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015149">
        <property type="term" value="F:hexose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045263">
        <property type="entry name" value="GLUT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23503:SF103">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23503">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="96"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="130"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="169"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="242"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="277"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="306"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="338"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="374"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="406"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="1"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00044504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7726829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="447" mass="49101" checksum="DF25A04361C0F9FF" modified="2024-05-29" version="1">MLVTATTLTHRWITPFSGIRTPPPLTPFPYPNQSLHPPLRLPTSSSLTTHFNPYYYYYCTNKSHLPQLIHSTSDKGELVTEEEINRNDWLPAFPHILVASMSNFLFGFHIGVMNGPILSIAKELGFEGNSILEGLVVSIFIAGAFLGSAGAGYVVDRFGFRRTLQVDTLPLILGAILRMLYSLIVLFLKAGRLDDAKKVISNLWGPSEVEKSIEEFQSVLKNDGGDLDSSWSELLEEPHSRVAFIGGALFVLQQFAGINAVLYFSSLTFKDAGITNGALASLYVGLTNFAGALCGLYLVDRQGRQKLIIGSYLGMAISMFLVVYAISFPIDDDLRSNLSILGTILYMLTYAIGAGPVTGLIIPELSSSRTRGKIMSFSFSVHWVCNFLVGLFFLDLTENFGVAPVYGSFGVVSILASIFSYSFLVETKGRSLEEIEISLNSNFAKRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BS32</accession>
    <name>A0AAD5BS32_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_020169</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011434">
        <property type="protein sequence ID" value="KAI7727144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BS32"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45631">
        <property type="entry name" value="OS07G0107800 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45631:SF202">
        <property type="entry name" value="SENESCENCE-INDUCED RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="61"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="115"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7727144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="364" mass="40894" checksum="EF8A1F64D6299EA2" modified="2024-05-29" version="1">MHGLPCSNLIGNNFTKPLPAQLLAKQKKGTLLLSIEESSDEDKSSCQEGSCGNNKHKKARFLVVTIAIGLSVVVLLSLVIIWLVKRRKKALIKRDESFIHRKQQFTYSEVVGITNNFQKEIGRGGFGGVFHGIYEDKQVAVKMLSESSSQGYKEFQAEVKLLMLIHRNITSLIGYCNDGEHKAIIYEFMANGNLEKHLYDGHPNVLSWERRLQIGCDAAEEGDWAPRAQSRFNRYLNEIHPGYYSTRRLTEKSDVYSFGVVLLELITGRKATLYGEHIVNWVKSPIENGNVETIIDSRIVGGFDINTVRKVVEMAMACVSSSSIERPTLDNVVVDLKNCLRAERERKSKLVSCNMDSVSGPTPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BSC9</accession>
    <name>A0AAD5BSC9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_023507</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Nudix hydrolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011191">
        <property type="protein sequence ID" value="KAI7728602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BSC9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016462">
        <property type="term" value="F:pyrophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04666">
        <property type="entry name" value="NUDIX_DIPP2_like_Nudt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.79.10:FF:000022">
        <property type="entry name" value="Nudix hydrolase 17, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047198">
        <property type="entry name" value="DDP-like_NUDIX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12629">
        <property type="entry name" value="DIPHOSPHOINOSITOL POLYPHOSPHATE PHOSPHOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12629:SF62">
        <property type="entry name" value="NUDIX HYDROLASE 17, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0464">Manganese</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="8">
        <location>
            <begin position="18"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7728602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="163" mass="19053" checksum="FD975B7E7B619C16" modified="2024-05-29" version="1">MMCMVSRTGRELQRYDMGRRLVVGCIPYRYKNENGELEVLVISSQKGHAMMFPKGGWELDESVEEAASRECFEEAGVVGVVECTLGKWSFKSKSRGIYREGHMFPMLVAEQLELWPEKNLRQRVWMKVEEAREVCQSWWMKEALDVFVERLASPTFNKQDFLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BSE3</accession>
    <name>A0AAD5BSE3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_028664</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011181">
        <property type="protein sequence ID" value="KAI7728625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BSE3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006461">
        <property type="entry name" value="PLAC_motif_containing"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01571">
        <property type="entry name" value="A_thal_Cys_rich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15907">
        <property type="entry name" value="DUF614 FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04749">
        <property type="entry name" value="PLAC8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7728625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="132" mass="14090" checksum="1EFE0FAAC5453E60" modified="2024-05-29" version="1">MYPQKQPPSPPPSAPPHLHNQPPPPPPQYASGIPAHYVAPPACTANWSSSLCACCSDVPNCCLTCWCPCITFGQIAEIVDKGNTSCGVHGALYGLIQALTGCGCIYSWWQGNIERQSYGVQMPPMAPGGMYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BTF6</accession>
    <name>A0AAD5BTF6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Vacuolar protein sorting-associated protein 54 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_019673</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VPS54 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011056">
        <property type="protein sequence ID" value="KAI7729170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BTF6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000938">
        <property type="term" value="C:GARP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006896">
        <property type="term" value="P:Golgi to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.860">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039745">
        <property type="entry name" value="Vps54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012501">
        <property type="entry name" value="Vps54_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12965">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING 54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12965:SF0">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07928">
        <property type="entry name" value="Vps54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Vacuolar protein sorting-associated protein 54 C-terminal" evidence="8">
        <location>
            <begin position="671"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="44"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="48"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="918"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7729170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="918" mass="101671" checksum="3A4486BBA289DDA6" modified="2024-05-29" version="1" fragment="single">IPEFATPVSNPKTSAGLELSRSDFASYLSSVLNHYSRFEHIINHSNKESNNNNNNDKNNNNNDDDDDENVERAGEALAACLKEVPALYFKEDFELEDGAMFRAVTGDASVGGDEEKLSQYLDVVEVHLVKEISLRSSSFYEAQGRLEGLNLEIVEGCGRIRELKERIRVLDGDLVDTARQVQELNVTRSDLLALREKLRVMLYVNQSLAALKLLVESGDCAGALDVTDDLKHVLNGDQLTGLHCFRHLEDHVVAAINSVNSILSAEFMRVSVSDAGDKDMVILSKAKARIASPGNEEDNEENWNQEDTSSFHERLLPLIIGLLRTAKLPTVLRIYRDTVISDMKTAIKSVVAELLPVLLSRPLDSDFKSGDRIVDADGGGSSLANKLRSLSSESFVQLLETIFKIVQAHLLRAAEVKKAIEWMMSNLDGHYAADSVAAAIANGALTAEISSAADGQSGSSSLLTQKNLLKVSPNTSSSTMSKNFRADVLRENAEAVFAACDAAHGRWAKLLGVRALLHPRLRLQEFLNIYNISQEFVTATEKIGGRLGYSIRGTLQSQAKAFVDFQHESRMTKLKAVLDQETWVEVDVPNEFQAIVDSLFRLESDSEDDDHSKSTANSYNEVVASSAEQPSQSVDGNVDLNGQVNNIKTKNGHAKSSAHLISFRGVGYHMMLSEYIDMNNVLPALSSEVIHRVLEMLKFFNTRTCQLVLGAGAMQVSGLKSITSKHLALASQVISFVHAIMPEIRKVLFLKVPETRKGLLLSEIERVSQDYKVHRDEIHTKLVQIMRERLLVHLRGLPQIIETWNRVDETDIQPSQFAKSLTKEVGYLQRVLSRTLHEADVQEIFKEVTIIFDIQISDAFAQVDINTPQAKARLNCEIQHILGCIKSLPSAKLYNESGTPIAGQLESLIERISAESDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BVZ6</accession>
    <name>A0AAD5BVZ6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">60S ribosomal protein L3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_002274</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010693">
        <property type="protein sequence ID" value="KAI7730636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BVZ6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000079">
        <property type="entry name" value="60S ribosomal protein L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1430.10:FF:000001">
        <property type="entry name" value="60S ribosomal protein L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.960.10:FF:000001">
        <property type="entry name" value="60S ribosomal protein L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.960.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000351">
        <property type="entry name" value="Ribosomal protein L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1430.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.960.10">
        <property type="entry name" value="Ribosomal protein L3, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045077">
        <property type="entry name" value="L3_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044892">
        <property type="entry name" value="Ribosomal_L3_dom_3_arc_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000597">
        <property type="entry name" value="Ribosomal_uL3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019926">
        <property type="entry name" value="Ribosomal_uL3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11363">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11363:SF18">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00297">
        <property type="entry name" value="Ribosomal_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00474">
        <property type="entry name" value="RIBOSOMAL_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="18"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7730636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="389" mass="44374" checksum="D5DFE4C24D86D35F" modified="2024-05-29" version="1">MSHRKFEHPRHGSLGFLPRKRAARHRGKVKAFPKDDPTKPCRLTAFLGYKAGMTHIVREVEKPGSKLHKKETCEAVTIIETPPMVVVGLVGYVKTPRGLRSLNTVWAQHLSEDVKRRFYKNWCKSKKKAFTKYALKYENDSGKKEIQSQLEKMKKYCNVIRVLAHTQIRKMKGLKQKKAHLMEIQVNGGSVAQKVDFGYSFFEKQIPIDAVFQKDEMIDIIGVTKGKGYEGVVTRWGVTRLPRKTHRGLRKVACIGAWHPARVSFTVARAGQNGYHHRTEMNKKIYKLGKTGQETHSALTEFDRTEKEITPMGGFPHYGVVKDDFLMIKGCCVGPKKRVVTLRQSLLAQTSRLALEEIKLKFIDTSSKFGHGRFQTADEKAKFYGRTKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BW27</accession>
    <name>A0AAD5BW27_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Auxin response factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_023045</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ARF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010682">
        <property type="protein sequence ID" value="KAI7730681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BW27"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009734">
        <property type="term" value="P:auxin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10017">
        <property type="entry name" value="B3_DNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.330.10:FF:000001">
        <property type="entry name" value="Auxin response factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.330.10">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044835">
        <property type="entry name" value="ARF_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003340">
        <property type="entry name" value="B3_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015300">
        <property type="entry name" value="DNA-bd_pseudobarrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053793">
        <property type="entry name" value="PB1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31384:SF194">
        <property type="entry name" value="AUXIN RESPONSE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31384">
        <property type="entry name" value="AUXIN RESPONSE FACTOR 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02362">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01019">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101936">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50863">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51745">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0927">Auxin signaling pathway</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TF-B3" evidence="9">
        <location>
            <begin position="153"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="PB1" evidence="10">
        <location>
            <begin position="547"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="498"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="667"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7730681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="667" mass="73841" checksum="C0EF617A1EE59B0D" modified="2024-05-29" version="1" fragment="single">SDTEVGYEVYSNGDHSSLSLTSNSSNISLKSSNLVDVESSMYKELWHACAGPLTTLPNKGNVVVYFPQGHLEQMSTQSYVHLSTIDVPSLGLKPHIFCKVVDVQLLANKDNDEIYTKLRLLPLPEFTGKNLKEGEEEEGGGDMAVTKSTPQMFCKTLTASDTSSHGGFSVPRRAAEDCFPPLVERDYKQQRPNQELVAKDLHGVEWKFRHIYRGQPRRHLLTTGWSIFVSQKNLVSGDAVLFLSVLRRYKKKTWSIIVLRGKGGELRLGTRRATRPRIFLPETNMPNSNSFMEILAPVRWDEDVGNNNQERISPWEIDLSGVSMPLLSMHSSLRFKKLRTDLHSTPSEHHVAAWGGCFDFEESTRSSKVLQGQENIVSKMNNSLRFGAQPSMHAGKSANIVTNPNTTILGSNNTRFPTVLQGQEICSLKSLAGDTNGPWGPPRADVNIHQRPKDPGYYPLGLPVNQVVFGNGLTKDHNIVNGLKLQLQAPVDLGKTTVDRNPIVEGNSGSEKDDGGSDSNGSSCKLFGFQLNEAPPQMEDSQILSKRSCTKVHKQGNKVGRAIDLSKMSSYHELHSELESLFQMEGVLTRCDGAWRLLYTDKENDIMVVGDDPWDEFARMATKIHICSMEEVEKLMSGGVISDDTSCLEEAPAVVDTAKSSPVGKPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BW99</accession>
    <name>A0AAD5BW99_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_009105</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010703">
        <property type="protein sequence ID" value="KAI7730610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BW99"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="35"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7730610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="54" mass="6339" checksum="237C5F3B09A2F72E" modified="2024-05-29" version="1">MYSTIDINESTREATRCNEATTEARKRSEKHVKRFLLVFGLSGLWKVITYFIVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BWN6</accession>
    <name>A0AAD5BWN6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_029436</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010909">
        <property type="protein sequence ID" value="KAI7729833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BWN6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044822">
        <property type="entry name" value="Myb_DNA-bind_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47211">
        <property type="entry name" value="TRIHELIX TRANSCRIPTION FACTOR ASR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47211:SF2">
        <property type="entry name" value="TRIHELIX TRANSCRIPTION FACTOR ASR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13837">
        <property type="entry name" value="Myb_DNA-bind_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="27"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="170"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="185"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="207"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="220"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7729833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="300" mass="33782" checksum="094BB6B281CEAC8B" modified="2024-05-29" version="1" fragment="single">MSVERLSLSIGEEEVNHNNNKAPRLPRWTRQELLVLIQGKRFAESRVRRGRSAVLAEVEPKWSSVSSYCKSHGVNRGPVQCRKRWSNLAGDFKKIKEWESQINKEEADSFWVMRNDLRRERKLPGFFDREVYDILDAGGGDGPSSASLALSLAPEVVFDSGRTTDAALFSDKPLISDTENEDQGVPPPSPSPPPAPVPAAETSTGAEPRETDRKRKRDDDAAEEEEGEEETSLQKQLIEAMERNGRLLSSQLEIQNVNSQLDREQRKDEANTLFSVLNKLADAMSLYFARGVQQYKCPHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BWQ5</accession>
    <name>A0AAD5BWQ5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_002073</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010612">
        <property type="protein sequence ID" value="KAI7730852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BWQ5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055198">
        <property type="entry name" value="NSD_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22908">
        <property type="entry name" value="PHD_NSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="2">
        <location>
            <begin position="112"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7730852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="172" mass="19718" checksum="8660B7D9CDC694E4" modified="2024-05-29" version="1" fragment="single">TKKSVSMRISTNFRPPIAMTERNVVSSDVVNGDVWDVKGQTMTLIADFKEHKKVIWQTLERKIECVELHIATKLSPRLAALMFESVLPRCCCLKKNCRKYVDHRGTLQQAECLVCENVIYPGEEIPCVVRDCKGSYHLTCARDWLGFTQSSKAFKCPQHRANVMKYHLTRKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BWR7</accession>
    <name>A0AAD5BWR7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TPX2 central domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_014423</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010560">
        <property type="protein sequence ID" value="KAI7731056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BWR7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005880">
        <property type="term" value="C:nuclear microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030295">
        <property type="term" value="F:protein kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090307">
        <property type="term" value="P:mitotic spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060236">
        <property type="term" value="P:regulation of mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027330">
        <property type="entry name" value="TPX2_central_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009675">
        <property type="entry name" value="TPX2_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14326:SF15">
        <property type="entry name" value="OS06G0130200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14326">
        <property type="entry name" value="TARGETING PROTEIN FOR XKLP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12214">
        <property type="entry name" value="TPX2_importin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TPX2 central" evidence="2">
        <location>
            <begin position="179"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="278"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="278"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7731056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="392" mass="43593" checksum="75F6A63408D3CF1C" modified="2024-05-29" version="1">MEVEMEDDNNNSTALEFTFTAVEIDIDYEYDAPRFFDFARKETVHEAREAEMWFASAASYPPSPFALRLLSREQSENASCSTSLKGPDIAPEDSSIGSMALNNTASQLAKQNQERLMDNFKFQKSGNNTSVVESQAAKRQKLDRGHLCKVTDTNQQTNFVHKAPKREGNVDGDVGRGRLRITVPRPPDLATARRAQRIRRKDSSGNEHVATRAPGFRARPLNRKIFEAPSFLPQKSIPQLPEFQEFNLKTSERAVQDTSAGSSNSAYCDINSKAPQKPSFTFGVQSSSRESKGPHVTGAPKQEDCETIHRFKALPLNTKILSSKGDLGVFRSSKRETTVAMAFNFQTEKRAHHTPPVDLFNKLSLASKSGSQSNKLRLSRAPSIFSKKFGIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BXA2</accession>
    <name>A0AAD5BXA2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Ribophorin-2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_001451</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 4">Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 4">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the oligosaccharyltransferase (OST) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Endoplasmic reticulum membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the SWP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010835">
        <property type="protein sequence ID" value="KAI7730163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BXA2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008250">
        <property type="term" value="C:oligosaccharyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055375">
        <property type="entry name" value="Ribophorin_II_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055374">
        <property type="entry name" value="Ribophorin_II_3rd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056790">
        <property type="entry name" value="Ribophorin_II_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055373">
        <property type="entry name" value="Ribophorin_II_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008814">
        <property type="entry name" value="Swp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12640:SF2">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12640">
        <property type="entry name" value="RIBOPHORIN II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05817">
        <property type="entry name" value="Ribophorin_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23861">
        <property type="entry name" value="Ribophorin_II_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23860">
        <property type="entry name" value="Ribophorin_II_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25147">
        <property type="entry name" value="Ribophorin_II_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="371"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="405"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="431"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="domain" description="Ribophorin II N-terminal" evidence="5">
        <location>
            <begin position="9"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="Ribophorin II second" evidence="7">
        <location>
            <begin position="90"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="Ribophorin II third" evidence="6">
        <location>
            <begin position="204"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="Ribophorin II C-terminal" evidence="8">
        <location>
            <begin position="358"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU366029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF05817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF23861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF25147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7730163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="466" mass="50903" checksum="D0C1BD8CF6698AE0" modified="2024-05-29" version="1">MLYDGSYYFDDKHIDASGHQGPLSATSAVVRGLTAFASTSESLNIPEEKILGLARFFLGIGVPGNSKDLYYQIDTLSHLENNRQVVSAPLILSLPATVLSLTSKDKLKVKVSTVLGSTVPPLSVKLMQVFSSGSKDASVLKQELHFHPEEAIHTLDALPEGVDIGEYVFAFEIVLSDPEHKRKFATGGRTKVPVHVTGVVKVDDAKVTVLEGDIVESEKKLDLPGKNDLALSANHLQKLHLSFLLTTPLGKPFKPHQALLKLRHESGVEHIFVVGNSGKHFEITLDFLSLVEKFFYLSGQYDIELTVGDAVMENSFLQPLGSIELDLPKAPEKATQPPPQAVNPYLRYGPKAEIAHIFRAPEKRPPQEVSFAFLGLVLVPFLGFLIGLLRLGVNLKNFPTSTIPATFAILFHGGIAAVLILYVFFWLKLDLFTTLKALGVLGIFLMFVGHRTLSHLASASAKLKSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BXY1</accession>
    <name>A0AAD5BXY1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_002555</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010483">
        <property type="protein sequence ID" value="KAI7731279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BXY1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031982">
        <property type="term" value="C:vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072318">
        <property type="term" value="P:clathrin coat disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072583">
        <property type="term" value="P:clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23172">
        <property type="entry name" value="AUXILIN/CYCLIN G-ASSOCIATED KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23172:SF64">
        <property type="entry name" value="J DOMAIN-CONTAINING PROTEIN REQUIRED FOR CHLOROPLAST ACCUMULATION RESPONSE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="106"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="255"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="148"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="271"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7731279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="552" mass="62004" checksum="FDF9227CEADE0A16" modified="2024-05-29" version="1">MEQRVSAVRNSDSEIEFDDVFGGPPMWTRYSYGGSRMRSWDEMSGSVERPVFGEGYSSPRRRTSDNSFRRGDESVRWSSPKPTSLPAKMTKAAEIPVFGSNMHTHNNHLSRFSTPSTHILRNNSRQSANQTLLTRESSSKDSLYSTRSTEKDHVDHKSKAKTGSSSIDFHFSMHKWANVGVPSLMSLHEGKHTGSSKVSGLSIDNMHDSVFPNEEKHDTLAMKQEVNEHGHGLTTLSSLLRDEFERKGYEEAGTKNMEKVSGKKKLKAKKMNSSNKAESNKPGVGSPKNRVKLMVKDLFKMSNQENPPKTKTKVSSIWKTGAKKRIQEEVTDSTISNLDKHVEMMTDALKATNLGCDLRAESVKMATNASVTIPLDSRVPADDVKEEIKRSKRQKFAQNRSIRKLEDINIQKDASPASESIRNSFQASAETIDDLSFDYFQVEELAPVASEATMALDSKIHMWSSGRTGNIRSLLSTLQLVLWAESGWKPVALVDIIEPNALKKSYNRAMLCLHPDKLQQKGIDSHKKYIAEKVLDILQVLVLDEILLYTYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BYK7</accession>
    <name>A0AAD5BYK7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein uS7 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_019677</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010418">
        <property type="protein sequence ID" value="KAI7731609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BYK7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.455.10">
        <property type="entry name" value="Ribosomal protein S7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000235">
        <property type="entry name" value="Ribosomal_uS7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023798">
        <property type="entry name" value="Ribosomal_uS7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036823">
        <property type="entry name" value="Ribosomal_uS7_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11205">
        <property type="entry name" value="RIBOSOMAL PROTEIN S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00177">
        <property type="entry name" value="Ribosomal_S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47973">
        <property type="entry name" value="Ribosomal protein S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS7" evidence="4">
        <location>
            <begin position="36"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7731609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="176" mass="19921" checksum="F0D73F81CF938A42" modified="2024-05-29" version="1">MTPLLLQHYATAGNSKTAYTGSEILNYMGGLDGEKKMLIHKLVNFRMKQGKKTRVRAIFHETLHRLARTDRDGIELISDALENVKPVCEVAKVRIAGNIYDVPGVVDRDRQQTLAVRWILDEAIRAKKKTKNSSKLEECLFAQIMDAYRKRGEVLLHYGLVKKKIDGAGESIVKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BYQ6</accession>
    <name>A0AAD5BYQ6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_009185</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010387">
        <property type="protein sequence ID" value="KAI7731790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BYQ6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766:SF61">
        <property type="entry name" value="NB-ARC DOMAIN DISEASE RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766">
        <property type="entry name" value="PLANT BROAD-SPECTRUM MILDEW RESISTANCE PROTEIN RPW8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0611">Plant defense</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7731790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="402" mass="45767" checksum="467C33FCB543D12F" modified="2024-05-29" version="1">MSEWGPQSFPSSLVQLWLYDEVDVKNFCQLSHLFPSSLTKLNIVNFDKLESLSKGLQHLTSLQHLTIWYCPKLIHLPKQLLPKLLSLEIYGCPKLDKRCEGRGSHYYPLISHIPKIEDGPGDEDSDEDKSPDIRLAQSVAIETGVIKDSLRVGMATVTRDLDDVTFVNDLQRIYTNGKGHGLAEMVTELKEDKEDNILKDGESMVLAKQFWCAANENRNRRNIRHVPKVSYYRTDAKFQPKNSLTVMGILLFSFESELLNLSLSRNLYPKVMFPFDNKRKGRREVCPPHKNPCVRFDTVVQFLTDKCPDDIGLEQLVVLETSVIKDHPRVGMTTRDLDDVTSVNVHHRIDTNGSKISNLKQNGLVITVSPISKELIKIEEGPRYLGGFCPFDLTRDDYAKPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-10-08" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BZJ3</accession>
    <name>A0AAD5BZJ3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_023203</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010186">
        <property type="protein sequence ID" value="KAI7732648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03232">
        <property type="entry name" value="ABCG_PDR_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000157">
        <property type="entry name" value="ABC transporter G family member 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029481">
        <property type="entry name" value="ABC_trans_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034003">
        <property type="entry name" value="ABCG_PDR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013581">
        <property type="entry name" value="PDR_assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48040:SF12">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 32-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48040">
        <property type="entry name" value="PLEIOTROPIC DRUG RESISTANCE PROTEIN 1-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19055">
        <property type="entry name" value="ABC2_membrane_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14510">
        <property type="entry name" value="ABC_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08370">
        <property type="entry name" value="PDR_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="510"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="551"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="591"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="628"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="654"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="750"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1185"/>
            <end position="1203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1215"/>
            <end position="1238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1272"/>
            <end position="1288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1295"/>
            <end position="1313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1325"/>
            <end position="1345"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="135"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="836"/>
            <end position="1088"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7732648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="1370" mass="155789" checksum="95171C4B43AF115D" modified="2024-05-29" version="1">MWNSAENLSARSSSFREDGDDEEALRWAAIERLPTYNRVRKGIFTNIVGDSKEVDVNKLEIEELKLVLDRLLNCEDDDRKRFFDRMRRRFDAVDLEFPKIEVRFHNLNVETFVHVGSRALPTITNFIVNMTEAFLRQLRIYKGNGRKLTILDDVSGIIRPSRMTLLLGPPSSGKTTLLLALAGRLGNHLQVSGKISYNGHGLKEFVPQRTSAYVSQQDWHIAEMTVKETLDLSARCQGVGFKYGVHCRYAYXXXXXXXXXXXXXXXXXXXXXXMKALALGGHETSLVVEYILKILGLDTCANTLVGDEMLRGISGGQKKRLTTGELLVGPSRVLFMDEISNGLDSSTTYQIIQYLRHSTHALDGTTVVSLLQPAPETYDLFDDIILICEGHIVYQGPRNAALDFFASMGFHCPKRKNVADFLQEVVSEKDQEQYWSVPNRPYCYIPVKKFSEAFRSYRVGKNLVEELNVPFDRQYNHPAALSTSSYGVKRSELLKTSFNWQLLLMQRNSFIYVFKFVQLLFVAFITMSVFFRTTLHHRTVDDGGLYLGELYFSMVILLFNGFTEVSMLVAKLPVIYKHRDLRFYPCWVYTLPSWVLSIPTSLIESGIWVVVTYYVVGLDPNIIRFLKQFLLFFFLHQMSISLFRLMGSLGRNMIVANTFGSFAMLIVMALGGYVISRDSIPSWWIWGFWISPLMYAQDAASVNEFRGHSWDXXXXEHVSLKEVLMQGANQSVLTLGERVLKARSIFPQTYWYWIGLGALLAYTILFNALFTFFLSYLNPLGRSQAVVSKEQKDKDGKKNGESVVIQLREFLEHSGSFTGKNINQRGMVLPFQPLSMAFSNINYYVDVPLELKQQGVAVDRLQLLVNVTGAFRPGVLTALVGVSGAGKTTLMDVLAGRKTGGVIEGSIYISGYPKSQETFARVSGYCEQNDIHSPCLTVLESLLFSAWLRLPSDVDCKTQKIFVDEVMELVELTPLKGALVGLPGVDGLSTEQRKRLTIAVELVANPSIVFMDEPTSGLDARSAAIVMRTVRNIVNTGRTIVCTIHQPSIDIFEAFDELLFMKRGGQLIYAGPLGPRSCKLVEYFEAIEGVRKIKSGHNPATWMLEVTSPAEETRLGVDFAEVYRQSKLFTRNRELVERLKKPRNDAKALHFPTKYSRSYMDQFATCLWKQYLSYWRNPQYTAVRFFYTSIISLMLGTICWRFGSKREHQQDLFNAMGSMYLAILFIGITNATAVQPVVSVERFVSYRERAAGMYSALPFAFAQGAIEFPYVLAQALIYSTIFYSLAAFEWTTWKFIWYIYFMYVTMLYFTFYGMMTTAVTPNHNVAAIIAAPFYMLWNLFSGFMIPHKVINLKFVGTTRSSKQQKHKDYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C1E7</accession>
    <name>A0AAD5C1E7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_002359</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009944">
        <property type="protein sequence ID" value="KAI7733561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C1E7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="14"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7733561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="96" mass="11596" checksum="5DE256B5873C2645" modified="2024-05-29" version="1">MRFQWLVATEWLNARRLKRRLDRAKMMIKKKKCMKKQLKLQNPQIQVVQPLVMIKEKIRGNLVSWNYSTNSWMVPRLKSPVRIKANQVNWRGLTGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C303</accession>
    <name>A0AAD5C303_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_014618</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009845">
        <property type="protein sequence ID" value="KAI7734070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C303"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45136">
        <property type="entry name" value="ABC TRANSPORTER DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45136:SF2">
        <property type="entry name" value="ABC TRANSPORTER DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7734070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="82" mass="8857" checksum="E5DCCDF24A62299F" modified="2024-05-29" version="1">MFLMAVGLIRAIGDGLGTPVMFFFTSKVMNSIGSNSSASADVYTDKINKNAVRLCYLAIAKWVACFLVMREQVDSSVVTVEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C3B2</accession>
    <name>A0AAD5C3B2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NHL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_016003</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009934">
        <property type="protein sequence ID" value="KAI7733613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C3B2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001258">
        <property type="entry name" value="NHL_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13833">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13833:SF78">
        <property type="entry name" value="POTASSIUM TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01436">
        <property type="entry name" value="NHL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101898">
        <property type="entry name" value="NHL repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041966589" description="NHL domain-containing protein" evidence="3">
        <location>
            <begin position="28"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="373"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="376"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="431"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7733613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="509" mass="56877" checksum="F347B21D55AB3DC6" modified="2024-05-29" version="1">MISNNHVFYLPIIFLLFSASIISPASATPPAKLLSGIASNTLSALFKWLWSLKPDTKTNVAVTRSSMVKYEGGYSVETVFDGSKVGIEPYSVVVTPSGEVVVLDSENSNVYKISTPLSKYSRPKILAGSPEGLFGHVDGKPRESRMNHPKGLTVDDRGNVYVADTMNMAIRKISDAGVVTIAGGNLARGMGHIDGPSDNAKFSNDFDIIYVASSCSILVIDRGNQAIREIQLHEDDCSYQDDGEDHLGMAVLGVAMLFGFMLALLQRRVSALFSSQRDPRPVMNTIPPSAYQRPMRSVRPPLIPPEDEYEKEEDGLFVTLVKFLIKPVTTIFAIFFGSFSSKKKTSHTHLQMHYPQPLPYSNTWPMQESFVIPHQDVPPPLETRDPPPYPFMTNDNPEPAERPRPSKQTRYLNGGSGQNSECLTEQQVKQSQKHQHRQHHQKHHSSGSQTYYEKSESTNEVVFGAVQEQDRKHEAVVIKAVDYNNIPNYNQPNLRSRYSYMGYAYDTNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C404</accession>
    <name>A0AAD5C404_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">leucine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Leucyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">M8C21_030414</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">AA19_3_7</strain>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>tRNA(Leu) + L-leucine + ATP = L-leucyl-tRNA(Leu) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:11688"/>
            <dbReference type="Rhea" id="RHEA-COMP:9613"/>
            <dbReference type="Rhea" id="RHEA-COMP:9622"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78494"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.4" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010009755">
        <property type="protein sequence ID" value="KAI7734345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C404"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004823">
        <property type="term" value="F:leucine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006429">
        <property type="term" value="P:leucyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009791">
        <property type="term" value="P:post-embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048608">
        <property type="term" value="P:reproductive structure development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07959">
        <property type="entry name" value="Anticodon_Ia_Leu_AEc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.730.10:FF:000020">
        <property type="entry name" value="Leucine--tRNA ligase cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.740.10:FF:000001">
        <property type="entry name" value="Leucine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.740.10">
        <property type="entry name" value="Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004493">
        <property type="entry name" value="Leu-tRNA-synth_Ia_arc/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055416">
        <property type="entry name" value="RBD_LARS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00395">
        <property type="entry name" value="leuS_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45794:SF1">
        <property type="entry name" value="LEUCINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45794">
        <property type="entry name" value="LEUCYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24810">
        <property type="entry name" value="RBD_LARS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="ValRS/IleRS/LeuRS editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="12">
        <location>
            <begin position="24"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="12">
        <location>
            <begin position="194"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="domain" description="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase anticodon-binding" evidence="13">
        <location>
            <begin position="794"/>
            <end position="914"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine--tRNA ligase RagD-binding" evidence="14">
        <location>
            <begin position="944"/>
            <end position="1021"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="124"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1064"/>
            <end position="1087"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF24810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAI7734345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="1087" mass="123816" checksum="9A4B94CF8D7763B1" modified="2024-05-29" version="1">MADTDSGRSFKRRDQLLEMEQEVRKWWDESHVFNAEAKDQPPKEAEKFFGTFPFPYMNGYLHLGHAFSLSKLEFAAAYHRLRGANVLLPFGFHCTGMPIKASADKLRREIEKFGNPPVFPSVETEDVVVEPKPKPSDGSNPENFKGKKSKAASKTGRETYQWEIMRGYGLSDAEIATFQDPVQWLRFFPPLAVEDLKAFGLGADWRRSFITTDINPFFDKFVRWQMRKLKSFGKIVKDLRYTIYSPLDGQPCADHDRASGEGVIPQEYTLIKMEVVPPFPPKFAHLEGKKVYLAAATLRPETMYGQTNSWVLPDGKYGAFEINESEVFILTERAARNLAYQRLSRVHEKATCLVELTGQDLIGLPLRSPLSFNEVIYCLPMLSVLTDKGTGIVTSVPSDSPDDFMALQDLKAKAPFRAKFGVKDEWVLPFEVIPIISHPEFGDKSAEKICTDMKIKSQNEREKLDAAKKVIYKGGFYEGTMIAGEYAGMRVQDAKNLIRAKLLELNQAVVYSEPEKKVMSRSGDECVVALTDQWYLTYGESSWREAAEECLANMNLYSDETRHGFEHTLSWLNQWACSRSFGLGTRIPWDEEFLVESLSDSTIYMAYYTVCHLLQRGELFGADTSAVKPEQLTDEVWDYLFLSGPEPKSSDISLTLLKKMRQEFEYWYPVDLRVSGKDLIQNHLTFSIYNHTAIFPKRHWPRGFRCNGHIMLNSEKMSKSTGNFRTLKQAIEEFSADATRFSLADAGDGMDDANFVFETANAAILRLTKERVWMEEVLAAESSLRGGPPSTYADRVFANEMNFAIKMTEKNYGDYLFREALKTGFYDLQIARDEYRLSCGSSGMNRDLLWRFMDIQTRLIAPICPHYSEYVWRMLLKKEGFVIKAGWPEAENPDLTLQKANRYLQDSITNFRKLLLKQTSGGSKKGSNNKASIQSKPTMGLIFVNEQYDGWKRECLNILRDKFDNENRKFAPDQEILQALQQSAVGQEGNFKQVQKLCMPFLRFKKDEVMTLGVQALDLRLPFGEIEVLQENLELIKRQIGLEHVEILSATDSDAVVKAGRHASLLKQTPPSPGSPTSIFLTNDDLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C4E7</accession>
    <name>A0AAD5C4E7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AMP-activated protein kinase glycogen-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_009822</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009690">
        <property type="protein sequence ID" value="KAI7734570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C4E7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02859">
        <property type="entry name" value="E_set_AMPKbeta_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032640">
        <property type="entry name" value="AMPK1_CBM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47434:SF1">
        <property type="entry name" value="PROTEIN PTST HOMOLOG 2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47434">
        <property type="entry name" value="PROTEIN PTST HOMOLOG 3, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16561">
        <property type="entry name" value="AMPK1_CBM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-activated protein kinase glycogen-binding" evidence="1">
        <location>
            <begin position="244"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7734570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="324" mass="36828" checksum="00A72C6B16A71931" modified="2024-05-29" version="1">MVWFIATPSSLSATAAAARHLISPNGLKIRRPRVSLAATALERELLVFMKNSSNPNEFPTKNQLLDAGRLDLVNAVANIGGWLAFGWEDEDYSQSQHQLQTTTVEERSISKEDIGVEATKIDSQLKERREHNSKDLNLDKIRSRLQQMQLELSSAIHSLRSKNRTLNSAVHETHGSELRRLSYVWEFQENEVMKAGDKLRSIRAKLAVLEGKIALSVIDTQKLVEEKQRRIDSARTTLQHLRTTCIFWTHSASEVMLVGSFDGWTSQVKMEKTRTGIFSTSLKLYPGRYEIKFIVDGIWRVDRTLPVVHYNGFENNSLIVHESA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C5D9</accession>
    <name>A0AAD5C5D9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Stromal processing peptidase, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_030851</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009535">
        <property type="protein sequence ID" value="KAI7735194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C5D9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.830.10">
        <property type="entry name" value="Metalloenzyme, LuxS/M16 peptidase-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050626">
        <property type="entry name" value="Peptidase_M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43690">
        <property type="entry name" value="NARDILYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43690:SF33">
        <property type="entry name" value="STROMAL PROCESSING PEPTIDASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="LuxS/MPP-like metallohydrolase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="7">
        <location>
            <begin position="202"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 C-terminal" evidence="8">
        <location>
            <begin position="354"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 C-terminal" evidence="8">
        <location>
            <begin position="441"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 C-terminal" evidence="8">
        <location>
            <begin position="925"/>
            <end position="1134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7735194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1260" mass="141324" checksum="199DA993C21970AE" modified="2024-05-29" version="1">MEASSITHPLHLNHKPSSSFNKPTYTPSLRFLPGVNKTVTLRPFHPHSRRTTLAPPTYFTTKIAGDRCVFPLDKASGQRPLLDQCTRFSCFHHGRRKRFPICKSTPGAFSDKSIFRLSKHGVDNVYGKRVRVLCGAVGPDEPHAASTAWPEGVLEQQSSDNFMVDSENSEWRELQGFLGAQLPSHPKLVRGQLENGLRYLILPNKVPQNRFEAHMEIHAGSIDEEDDEQGIAHMIEHVAFLGSKKREKLLRTGARSNAYTDFHHTVFHIHSLTSMQDSDEDLLPSVLDALNEIAFYPKFVTSRVEKERRAILSELQMMNTIEYRVDCQLLQHLHSENKLSKRFPIGLEDQIKKWDAEKIRQFHERWYFPANATLYIVGDIDNIAKTISHIKAVFGPKHVDETPVAPKTSAFSAMANFLVPKFTGGISTDSLNDKASLLVNQSKLPKKERHAVRPPVEHNWSLPGRHEDLTPQIFQHELLQNFSINMFCKIPVNKVQTFGDLRNVLMKRIFLSALHFRINSRYKSSSPPFTSIELDHSDSGREGCTVTTLTVTAEPQSWQGAIKVATQEVRRLKEFGVTKGELARYLDALLKDSEQLATMCDNISSVDNLDFVMESDALGHMVMDHKQSFESLISVAGTITLEEVNFIGAQVLEYISDFGKPSAPVPAAIVACVPKKVHVDGVGEIDFKISPSEITAAIEEGLKQPIEPEAEFEVPKELITSTELDDLKLQLKPSFISTGLDSNATKVYDKETGITQCRLSNGISINYKTSKTEKCGGVMRLIVGGGRAAETPETKGAVVLGVRTLSEGGRVGNYTREQVELFCVNHLVNCSLESTEEFICMEFRFTTRDNGMRAAFQLLHMVLEHSVWLEDAFDRARQLYLSYYRSIPKSLERSTAHKLMIAMLDEDERFVEPTPYSLQNLTLQTVSIVGDFSEEDIESCVMDYLGTIRASRGADRALSYHPIIFRPSPHDLHFQQVFLKDTDERACAYIAGPAPNRWGYAVDGTDFLESISKVSANNDIQPETEGQLVKSDNIDFHMQRSRNHPLFFAIAVGLLAEIINSRLFTEVRDSQGLTYDVSCELNLFDRLNLGWYVISVTSTPSKIHRAVDACKNVLRGLHSKKIADHELDRAKRTLLMKHETETKSNAYWLGLIAHLQAASVHRKDISCIKDLTMLYETATIEDVYLAYEQLKIDDQSLYCCIGIAGAQANDEVSDSLQEDEYVGGLHGVLPVGRGSHAVPSGDVSNDVFITGSCVCNEFVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C6M5</accession>
    <name>A0AAD5C6M5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_023689</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009381">
        <property type="protein sequence ID" value="KAI7736072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C6M5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7736072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="103" mass="11671" checksum="06789522542A15A9" modified="2024-05-29" version="1">MMNKEANLEGKRIIYEDLKAGKRWKTLCPIFYNFQNQGSWAKITPAKGISIWQREFVGGRPEASLSSSLALKGKQDRLLNSDTRARLNLSILIETKGVLLEAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C724</accession>
    <name>A0AAD5C724_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Tubulin beta chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">M8C21_000658</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">AA19_3_7</strain>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11 12">Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="12">Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the tubulin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009406">
        <property type="protein sequence ID" value="KAI7735935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C724"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02187">
        <property type="entry name" value="beta_tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.600:FF:000002">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.20:FF:000002">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000005">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.600">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013838">
        <property type="entry name" value="Beta-tubulin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002453">
        <property type="entry name" value="Beta_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023123">
        <property type="entry name" value="Tubulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01163">
        <property type="entry name" value="BETATUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00228">
        <property type="entry name" value="TUBULIN_B_AUTOREG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9 12" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 12" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="14">
        <location>
            <begin position="48"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="15">
        <location>
            <begin position="247"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="423"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="430"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAI7735935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="446" mass="49928" checksum="9384C65317A281ED" modified="2024-05-29" version="1">MREILHIQGGQCGNQIGAKFWEVTCDEHGIDPTGQYKGSNADLQLERINVYYNEASGGRYVPRAVLMDLEPGTMDSIRSGPYGKIFRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDVVRKEAENCDALQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVVEPYNATLSVHQLVENADECMVLDNEALYDICLRTLKLTTPSFGDLNHLISVTMSGVTCCLRFPGQLNSDLRKLAVNLIPFPRLHFFMVGFAPLTSRGSQQYASLSVPELTQQMWDAKNMMCAADPRHGRYLTASAMFRGKMSTKEVDEQMINVQNKNSSYFVEWIPNNVKSSVCDIPPTGMKMSSTFIGNSTSIQEMFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVAEYQQYQDATADDYEDEEEGGEEEDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C745</accession>
    <name>A0AAD5C745_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_022680</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009328">
        <property type="protein sequence ID" value="KAI7736230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C745"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052650">
        <property type="entry name" value="Zinc_finger_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36886">
        <property type="entry name" value="PROTEIN FRIGIDA-ESSENTIAL 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36886:SF3">
        <property type="entry name" value="PROTEIN FRIGIDA-ESSENTIAL 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="182"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="225"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="44"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="182"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="193"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="232"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="246"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7736230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="390" mass="43049" checksum="AFB4962EC61316F9" modified="2024-05-29" version="1" fragment="single">FRHIKDSPINVVDQQTEPQKNKPQDEGLVAIPGVKESTGRSDDLATSATASTLIASSAEYKPGPQKDNPNTVKNSPVNKSIGNPFMGPNTHRSSWASVAPKVQEFSGKGYHLGLHNHGYNSSYHGTVWPKTSTFLSSSSRKSDSKVFSNNWELSKPFRSTFLISQGISTPDIQYDPIRDSIEQPAKNGDKLSKVSSSSRVPSISGTHSPLQEKLRTDFGSDRLSIGSHVNGNDDDIDMDVDSNEIADTKQENTKSESKGKGKRHIGDVIQAKSGHSSLRQNDDGAAKEVNNYKLDFDAEGDMYRESKSLRHFRASLIEFVKELVRPSWRDGKLSKDAHKLIVKKAVDKVLTSLPPEHVPDIKESIDAYLTSSQPKLMKLVEGYIEKYGKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C983</accession>
    <name>A0AAD5C983_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_009051</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009134">
        <property type="protein sequence ID" value="KAI7737060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33477">
        <property type="entry name" value="P-LOOP NTPASE DOMAIN-CONTAINING PROTEIN LPA1 HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33477:SF3">
        <property type="entry name" value="P-LOOP NTPASE DOMAIN-CONTAINING PROTEIN LPA1 HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13671">
        <property type="entry name" value="AAA_33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="227"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="450"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="232"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="464"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7737060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="670" mass="74401" checksum="EB170A38DDCD4C3F" modified="2024-05-29" version="1">MEVGKLLYIVVEDDDNNIGGHVNEELCFRYTRHVLQSTLQLMGCKARHAFKISQRVFEIMRTECLGDTLAAMSVIKTEGGGSSNTVLKNDDEDDKLSSSIPFESYKKRTTVIVKRRRFVDVVCDALAEYKYVGPNQRADLVLACRIRERKESMTVLLCGTSGCGKSTLSALLASRLGITTVVSTDSIRHMMRSFVDEKQNPLLWSSTYHAGEHLDVVAVSEAKAKRKAKKNGSVSPQSQFKNAPFDASAPEGLTTVDLISPKQMAIEGFKAQSEMVIDSLDRLITAWEERKESVVVEGVHLSLNFVMGLMKKHPSIIPFMIYITNEDKHMERFAVRAKREAGEQLCDSTTNTVSVVNEEYRNQCIANSLSSKGMFQLIQRQGSSRNLMALLNDDGSVAKVWPVHSVDADGKPIIGHPDYGPLQIGKAEPVNLQFGNFGISAWPTDVGCTSQAGSVDESRGELTDNGSRYHSSGCSSPKASDGPSKELKEEQSVYGSDEEGDDNXXXXXXXXXXXXXXXXXXXXXXXXXXXXVIDNVVYILFEQMEGSVDEESTKSDEEYDDLAMQDIQENGYLNEDELDQKHNLIPPTQHDTFANYDIDKYSQNLDRFLRAKSEPHWDSVQPNPSIIKAEKMVVSSGSLIVKRRSHSVSCFGKDVSLSLTHDDGYCYTIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C9M5</accession>
    <name>A0AAD5C9M5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_012081</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009258">
        <property type="protein sequence ID" value="KAI7736476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C9M5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7736476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="84" mass="9707" checksum="3317B48EF956B74E" modified="2024-05-29" version="1" fragment="single">MFTNLRLVISTLKSMLIPRSCRMTSTCLTTMSKNELMLSWLQHCHRQTLLGRIMLCGQWGQILNISMQSHGFGIWTSLFIMSIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CAL1</accession>
    <name>A0AAD5CAL1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_026802</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009109">
        <property type="protein sequence ID" value="KAI7737134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CAL1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050665">
        <property type="entry name" value="Cytochrome_P450_Monooxygen"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24282:SF254">
        <property type="entry name" value="CYTOCHROME P450 CYP72A219-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24282">
        <property type="entry name" value="CYTOCHROME P450 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11 12" id="KW-0349">Heme</keyword>
    <keyword evidence="8 11 12" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 12" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="7 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="binding site" description="axial binding residue" evidence="11">
        <location>
            <position position="327"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7737134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="389" mass="44334" checksum="B8C733DBE90D0E9F" modified="2024-05-29" version="1">MGPVPTVHITEPAMIKEVFANNYQFQKPRGGNPLIKLLATGLADAECDRWSKHRKIINPAFHVEKLKVCSYCLKFLNHNNFEEGKRIFELQREQAELVIEAIQSIYIRGSRFLPTKRNKRMKEIEREVKGSIRNIINKRIIAIKNGESSAEDLLGILLDSNYKAIKHGNKNSGLSIDEIIEECKLFYFAGQETTGNLLLWTMVLLAQHTDWQAKARDEVSLIFGNKRPDIDGLNRLKTVSMIFSEVLRLYPPVDSLTRMIHQETKIGNITLPAGSILQLHTLLTHHDRDIWGDDVKEFKPERFYEGVSKVTKGQASYIPFGGGPRICIGMNFALLEAKTALSMILQHFRFDISPSYSHAPYSMVTLQPQLGAHCQEMESKRLNIEFVIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CAY8</accession>
    <name>A0AAD5CAY8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DM2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_010301</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008879">
        <property type="protein sequence ID" value="KAI7738089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CAY8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd10567">
        <property type="entry name" value="SWIB-MDM2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.245.10">
        <property type="entry name" value="SWIB/MDM2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019835">
        <property type="entry name" value="SWIB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036885">
        <property type="entry name" value="SWIB_MDM2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003121">
        <property type="entry name" value="SWIB_MDM2_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13844">
        <property type="entry name" value="SWI/SNF-RELATED MATRIX-ASSOCIATED ACTIN-DEPENDENT REGULATOR OF CHROMATIN SUBFAMILY D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02201">
        <property type="entry name" value="SWIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00151">
        <property type="entry name" value="SWIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47592">
        <property type="entry name" value="SWIB/MDM2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51925">
        <property type="entry name" value="SWIB_MDM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DM2" evidence="2">
        <location>
            <begin position="62"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7738089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="144" mass="15800" checksum="47BF7E8D7B3D1B99" modified="2024-05-29" version="1">MAMPLGFLSTTFSATGTPSLLKSPSLSVQAVSFNAAKLRTSVTYATASSEPATDTKKRAPRGFSKPRPVSPEMRDFLGGRTEIPRTEVLKVIWAHIKQNGLQDPNNKRVIVCDEKLKKIFGEKDQVGFLEIAGLISPHFKKVEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CBF7</accession>
    <name>A0AAD5CBF7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_025111</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the major facilitator superfamily. Phosphate:H(+) symporter (TC 2.A.1.9) family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008843">
        <property type="protein sequence ID" value="KAI7738248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CBF7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17315">
        <property type="entry name" value="MFS_GLUT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000152">
        <property type="entry name" value="Plastidic glucose transporter 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF2">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="70"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="144"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="177"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="204"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="288"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="324"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="352"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="384"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="420"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="449"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="8"/>
            <end position="474"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7738248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="489" mass="51975" checksum="912EBD305CA8136A" modified="2024-05-29" version="1">MNQKEARSCFGFGFGSDPTGLELGRVGNSTSGGTMFRSSVKPRSVKSQLSDGDIEESSLLKGQTTSSGSVLPYVGVASLGAILFGYHLGVVNGALEYLAKDLGIVENTVLQDHFGRTKTFVLDAIPLTLGAILCAMASNVETMIIGRLLSGVGIGISSAIVPLYISEISPTEIRGTLGSINQLFICIGILAALVAGLPLARNPLWWRTMFGIAAIPSVLLALGMAFSPESPRWLVQQRKISQAEQAIRKLYGKNRVTELMEDLSASGQGSEEHDAGWFDLFSSRYFKVVSVGAALFLFQQLAGINAVVYYSTAVFRSAGVTSDVAASALVGAANVFGTIIASSLMDKKGRKSLLITSFTGMAASMLLLSLSFTWKVLTPYSGPLAVIGTILYVLSFSLGAGPVPALLLPEIFASRIRAKAVALSLGMHWISNFFIGLCFLSVVNTFGISKVYLGFSCICVLAVMYIATNVVETKGRSLEDIERQLSPPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CBH7</accession>
    <name>A0AAD5CBH7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Mon2 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_011626</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008777">
        <property type="protein sequence ID" value="KAI7738622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032817">
        <property type="entry name" value="Mon2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34199:SF4">
        <property type="entry name" value="ARM REPEAT SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34199">
        <property type="entry name" value="NUMOD3 MOTIF FAMILY PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16206">
        <property type="entry name" value="Mon2_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mon2 C-terminal" evidence="2">
        <location>
            <begin position="385"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="domain" description="Mon2 C-terminal" evidence="2">
        <location>
            <begin position="831"/>
            <end position="1098"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="777"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="804"/>
            <end position="816"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7738622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="1171" mass="130378" checksum="60D5EF78DB2DC6B5" modified="2024-05-29" version="1">MMHSLEVSPNLQLCFGVLNWKIHSSESHDSSNTITFPYITKTTVPKTVIPKRHRQLLSYGQGELEYVLDPFNIMDIDPNQVFISFRTLQSPGSKRTEIVVDQRDVVVLTLKNFQALRTLFNITHRLYNVLGPSWVLVLETLAALDRALNSPHATTQEVSAAASKLTREPSGQYSDFSILSSLNSQLFESSGLMNISAVKSLLSALRQLSYQSMAGTLSGISQTSSQKTGSISFAVERMISILVNNLHRIQPLWDEVVGHFIELANSPNHHLRAMALNALDQSISAVLGSDQFEENALSRHHGIKTEMKALEISVIAPLHVLYDSCPNSDVNAGSLKILLHVLERHGDKLCYSWPNILDMLRSVAGSSEKDIVTLGFQSLRVIMNDGLSTVPSEFLHVCIDVTGAYSSQKTELNIXXXXLWTSTDFIAKGLLEGPIDDNGKATSEHMNVEKIEQTGNSVKKVNQQDSLITVAEHDKLLFSVFSLLQNLGADERPEVRNSAVRTLFQTLGSHGQKLSKSMWEDCLWNYVFATLDRASHMAATSSKDEWHGKELGVRGGKTVHMLIHHSRNTAQKQWDETLVLVFGGIARILRTFFPLLRSITNFWSGWESLLQSVKNSIANGSKEVALAAVGCLQSTVLSHSPKASYFWGYKSFLGNLPMPYLKSVLDVYDTVLRNPTACGDMATNKVKQEIIHGLGEVYVHAQGMFDKSMYAQLLSVIDSAIREAKTTQNNFEAEFGHVPPVQRVVLEVIPQLRPPHHLPSLWAVFFQKLLRYLPNSDSSEQNGDDAKPVESKGYAFDSNGTTTSDQVEVESSSSSSDSRKASAMTITSDLFAEKLVPVLVDMFLQAPVTEKFIIFPYIIQGLGRYIFFTATTAYLNRCMITRRENPDGGLWGLAVKSFNQLLVDDINNLANGSRLDVSNSNKPARIRLWKEVADVYEIFLVGYCGRALPSSSLAAISKEDDESLEMELLDVLGDKILLSDIDASPDILERLIITLDRCASRTCSLPVETVELVPPHCSRFSLTCLHKLFSLSCYNNELNNWNPTRSQVSKISVMILVTRCEYILKKFLIDEKELGERSFPPARISEIAFVLQEMARVVLHPETASVLPLHPFLKGGGQLEENSGQRAHLFVLFLPLCELVKSRNSRVRDLVHTLLKLVSTELGVHNICLTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CC43</accession>
    <name>A0AAD5CC43_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_000318</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008755">
        <property type="protein sequence ID" value="KAI7738668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CC43"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7738668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="57" mass="6566" checksum="7F377F2F5B5320F1" modified="2024-05-29" version="1">MIFLEGLYLASASVRSAIFNLVPAITFVAATIVGSRQLFRFLHHLTNHRKRLYYIID</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CCL5</accession>
    <name>A0AAD5CCL5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">60S ribosomal protein L10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_017903</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008747">
        <property type="protein sequence ID" value="KAI7738710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CCL5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010224">
        <property type="term" value="P:response to UV-B"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01433">
        <property type="entry name" value="Ribosomal_L16_L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1170.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.10">
        <property type="entry name" value="Ribosomal protein L10e/L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047873">
        <property type="entry name" value="Ribosomal_uL16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018255">
        <property type="entry name" value="Ribosomal_uL16_CS_euk_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016180">
        <property type="entry name" value="Ribosomal_uL16_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001197">
        <property type="entry name" value="Ribosomal_uL16_euk_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036920">
        <property type="entry name" value="Ribosomal_uL16_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003239">
        <property type="entry name" value="PRK04199.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00279">
        <property type="entry name" value="uL16_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11726">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00252">
        <property type="entry name" value="Ribosomal_L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54686">
        <property type="entry name" value="Ribosomal protein L16p/L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01257">
        <property type="entry name" value="RIBOSOMAL_L10E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7738710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="223" mass="25294" checksum="08FF387FB916CC3F" modified="2024-05-29" version="1">MFYYPGPARCYRQIKNKPYPKSRYCRGVPDPKIRIYDVGMKKKGVDEFPFCVHLVSWEKENVSSEALEAARIACNKYMTKFAGKDAFHLRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGVCARVSIGQVLLSVRCKDTNSPHAQEALRRAKFKFPGRQKIIVSRKWGFTKFSRTDYLQWKSENRIVSDGVNAKLLGCHGPLANRQPGRAFLDAAVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CCX6</accession>
    <name>A0AAD5CCX6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PITH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_014550</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PITHD1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008871">
        <property type="protein sequence ID" value="KAI7738141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CCX6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.470">
        <property type="entry name" value="PITH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045099">
        <property type="entry name" value="PITH1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010400">
        <property type="entry name" value="PITH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037047">
        <property type="entry name" value="PITH_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12175">
        <property type="entry name" value="AD039 HT014 THIOREDOXIN FAMILY TRP26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12175:SF1">
        <property type="entry name" value="PITH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06201">
        <property type="entry name" value="PITH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51532">
        <property type="entry name" value="PITH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PITH" evidence="2">
        <location>
            <begin position="9"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00025788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7738141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="204" mass="22574" checksum="69E52DA6F243DAA9" modified="2024-05-29" version="1">MACLHDHSCEDHDCSSDWSLYKHIDIPKVSALNEAVPGSVKSVFKAWEQRLSSSEGFLESNEGDPELIVFIPFTSDVKIKSISIVGGADGTSPIKMRAFINREGIDFSDAQSMQAVQEWDLAENLQGVLEYQTRYSRFQGVGNLTLHFPDNYGGDTSRIQYIGLKGEATQLKRDVVATIVYELMPNPSDHKTKSETGGGMSHVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CD89</accession>
    <name>A0AAD5CD89_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF3700 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_030428</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008631">
        <property type="protein sequence ID" value="KAI7739343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CD89"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024286">
        <property type="entry name" value="DUF3700"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044828">
        <property type="entry name" value="TSJT1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45952">
        <property type="entry name" value="ALUMINUM INDUCED PROTEIN WITH YGL AND LRDR MOTIFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45952:SF8">
        <property type="entry name" value="STEM-SPECIFIC PROTEIN TSJT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12481">
        <property type="entry name" value="DUF3700"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01172">
        <property type="entry name" value="DUF3700"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF3700" evidence="1">
        <location>
            <begin position="2"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7739343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="254" mass="28211" checksum="41130BECD617EC8B" modified="2024-05-29" version="1">MLAVFENKIANPPEELSLPFKGSGNLKIREQIAALVSSGRADLTRFAFSNGDFMALSHQDEHATCPRSSVVIDDIFCIFNGYLHNTCDLRRTYGLSRQATEAMVVVEAYKVLRDRAPYPADQVIKDLDGKFSFILFDSKATKLFLARHDRDGSAEIHWGVSADGCLVCSDDAEIVRKRCGDRYTPFPPGCLFKSEEGLISFDHPLNEVRGIVRVEEEGCSSAVIFQVDLYTQRYSIPRKGSDANWASVSVGEGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CDI9</accession>
    <name>A0AAD5CDI9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_004180</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008542">
        <property type="protein sequence ID" value="KAI7739967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CDI9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012438">
        <property type="entry name" value="DUF1639"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33130:SF63">
        <property type="entry name" value="DUF1639 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33130">
        <property type="entry name" value="PUTATIVE (DUF1639)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07797">
        <property type="entry name" value="DUF1639"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="58"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="58"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7739967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="146" mass="17027" checksum="54775D4DACA37F18" modified="2024-05-29" version="1">MLHKCATDEQLKVWPPSASVIDNLVLKLKSKHGDMDANWEKTILNFKKELQQEEEQLKAASSSCVDNNNNNNNNKGRPKFCVPLSRREMEKDFEDMGERRLPRKPKKRPKIIQNQLDTLFPGLWLTEIHADLYRVSETKKVQYFGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CEK6</accession>
    <name>A0AAD5CEK6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Kinesin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_027282</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008666">
        <property type="protein sequence ID" value="KAI7739174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CEK6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968">
        <property type="entry name" value="CENTROMERE PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968:SF35">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIN-7D, MITOCHONDRIAL ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 2" id="KW-0505">Motor protein</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="4">
        <location>
            <begin position="31"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="111"/>
            <end position="118"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7739174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="194" mass="21411" checksum="DE3EDF217F4D2706" modified="2024-05-29" version="1" fragment="single">MYSKDGYGGRSPVRFPSSDELISEPGGGGDSISVTVRFRPLSEREYQRGDEIAWYADGDKLVRNEYNPVTCYAFDRVFGASAVTQEVYEVAARPVVKTAMEGVNGTVFAYGVTSSGKTHTMHVRVVATRVKGQGDNHSPGIIPLAIKDVFSIIQDTPGREFLLRVSYLEIYNEVINDLLDPTGQNLRVREDAQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CF89</accession>
    <name>A0AAD5CF89_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Niemann-Pick C1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_017478</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008326">
        <property type="protein sequence ID" value="KAI7740897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CF89"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015918">
        <property type="term" value="P:sterol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032190">
        <property type="entry name" value="NPC1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45727">
        <property type="entry name" value="NPC INTRACELLULAR CHOLESTEROL TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45727:SF2">
        <property type="entry name" value="NPC INTRACELLULAR CHOLESTEROL TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16414">
        <property type="entry name" value="NPC1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="161"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="Niemann-Pick C1 N-terminal" evidence="2">
        <location>
            <begin position="5"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7740897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="186" mass="20259" checksum="42F06A72DF6CAE0A" modified="2024-05-29" version="1" fragment="single">PDDLLSAKVQSLCPTITGSICCTEAQFDTLRSQVQQAIPFLVGCPACLRNFLNLFCELTCSPNQSQFINVTSISQTKNNSTVDGIDYYITDTFGEGLFDSCKDVKFGSANTKAIDFVGSGAKNFKELFAFLGQKAAPKLPGSPYAINFRSDADVSVGMKPMNVVLVVIVLRLLFVPVLPLNHFMKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CGU6</accession>
    <name>A0AAD5CGU6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_001302</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008485">
        <property type="protein sequence ID" value="KAI7740226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CGU6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042209456" evidence="1">
        <location>
            <begin position="17"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7740226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="116" mass="13265" checksum="D6960FA92EF1D67B" modified="2024-05-29" version="1" fragment="single">TRAVTCLIVFMRALCADENIDDLTNANKVHHQVIKETRMLEARNKLKECQSKVDCWENAVSYGQFNEGGRKALPVFACSSPRLWDTTLNHVVLSVRVVFKLWDNQIVDLKYGRGNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CHJ9</accession>
    <name>A0AAD5CHJ9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_028351</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008056">
        <property type="protein sequence ID" value="KAI7742188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CHJ9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052591">
        <property type="entry name" value="CML21-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23064">
        <property type="entry name" value="TROPONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="54"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="145"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="183"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7742188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="227" mass="25886" checksum="602AECA583083BC6" modified="2024-05-29" version="1">MGGILTKGDDSSLTSVPTTKLELKILETMRRRESKGTSMKSFNTILLKFPKIDAGLRKCKAIFEEFDEDKSGSIDPKELNHCFRKLEIDFTDEEINDLFEECDINKDMGIAYKEFNVLLCLVYLLKKDSVSGHSSSRMEIPDLEATFETLVDSFVFLDKNKDGYVSKGEMVHAINESTSGEGSSGRIAMKRFEEMDWDKNGMVNFKEFLFAFTKWVGIEDEEEATEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CHP4</accession>
    <name>A0AAD5CHP4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_003250</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008133">
        <property type="protein sequence ID" value="KAI7741817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CHP4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007789">
        <property type="entry name" value="DUF688"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34371:SF2">
        <property type="entry name" value="DUF688 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34371">
        <property type="entry name" value="OS01G0551000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05097">
        <property type="entry name" value="DUF688"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7741817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="192" mass="21557" checksum="E44756A33E603504" modified="2024-05-29" version="1">MTSKSDTEKPDPYPTPKLPLFTSIPPPHHHSKPSGTSTPPFETTVSIPFHWEQQPGIPKPHSSTNHIIITHPSKCLHLPPRLLLSTTTKPILSPSPTTVLHGPERRRTQRTHRSFDSDGDGDGGGQNRWLLGDCKVTSSSTSAMESGCGKGRKMKMKRKWNESGSRFWVKIYEGFKKVVPWRKKPKIECFSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CIN7</accession>
    <name>A0AAD5CIN7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_009328</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007958">
        <property type="protein sequence ID" value="KAI7742562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CIN7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007019">
        <property type="term" value="P:microtubule depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47971:SF8">
        <property type="entry name" value="KINESIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47971">
        <property type="entry name" value="KINESIN-RELATED PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0493">Microtubule</keyword>
    <keyword evidence="4" id="KW-0505">Motor protein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="19"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7742562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="221" mass="23552" checksum="EB07C65B164466D5" modified="2024-05-29" version="1">MGGQMQQRNASATSLYDQPADGNAAPAGDAGDAVMARWLQSAGLQHLGSPVASNGVDQRLLPNLLMQGYGAQSAEEKQRLFKLMRNLNFNSDSGSESFSPTTQGHGGIGASDGFYSSEFRGDFGAGLLDLHAMDDTELLTENVMSEPFEQSPFIPVTTKAVNNDYDALPNRQQRGQIDVDASPGLPILEKEINSRENNVAKIKVVVCSSSLSLSLVVISST</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CK02</accession>
    <name>A0AAD5CK02_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_030859</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010007762">
        <property type="protein sequence ID" value="KAI7743252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CK02"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071244">
        <property type="term" value="P:cellular response to carbon dioxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902456">
        <property type="term" value="P:regulation of stomatal opening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009637">
        <property type="term" value="P:response to blue light"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010114">
        <property type="term" value="P:response to red light"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001659">
        <property type="term" value="P:temperature homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd13999">
        <property type="entry name" value="STKc_MAP3K-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000034">
        <property type="entry name" value="Kinase suppressor of Ras 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000310">
        <property type="entry name" value="Serine/threonine-protein kinase HT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF274">
        <property type="entry name" value="DUAL-SPECIFICITY KINASE TKL-PL-4 FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="75"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7743252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="382" mass="42421" checksum="96A63B4D61031A88" modified="2024-05-29" version="1">MGSSPKRGSGQLSFSSKELGGNISSKDTFLRADMIDFKSWDVQLDKHLSKVWSSDRDVKEVNDKKQEWEIDLAKLDIRNVIAHGTYGTVYRGVYDGQDVAVKVLDWGEDGLATAAETANLRTSFRQEVAVWHKLDHPNVTKFVGASMGTSDLKIPLNNASNPGHNSLPSRACCVVVEYLPGGTLKKYLIRNSRKKLSFKIVVQLALDLARGLSYLHSKKIVHRDVKTENMLLDSNRTLKIADFGVARVEAQNPRDMTGETGTLGYMAPEVLDGKPYNRKCDVYSFGICLWEVYCCDMPYADLSFAEVSSAVVRQNLRPDIPKCCPSTFANILRKCWDANPDKRPEMDEVVRLLEAIDTSKGGGMIPEGQATGCLCFTTKRGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CKC7</accession>
    <name>A0AAD5CKC7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_024865</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007766">
        <property type="protein sequence ID" value="KAI7743227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CKC7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7743227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="211" mass="22977" checksum="EC5A3C0772289746" modified="2024-05-29" version="1" fragment="single">TLPRKTLDIKSHPCLLLLFLPPDFLKSPLLIVSLSSQPTRLTVDAITRLPDRETRPNGENTDQPTTCNWGVADYYSKKWAKSLKLRPNTHLSVTRSPSSSTMSSRLHLLKSLLSHDDDELLKLIIPHQVKYGSAGQRLRALMIASMLAVNHSSSESLSVCLLSTFVCTGAGGELLDLCVVWGVCMIFPSSVGGELMPVVIVLGVEDGVNGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CKY2</accession>
    <name>A0AAD5CKY2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_020094</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007811">
        <property type="protein sequence ID" value="KAI7743115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CKY2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05285">
        <property type="entry name" value="sorbitol_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000068">
        <property type="entry name" value="Sorbitol dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045306">
        <property type="entry name" value="SDH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43161:SF17">
        <property type="entry name" value="L-IDONATE 5-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43161">
        <property type="entry name" value="SORBITOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="8">
        <location>
            <begin position="19"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7743115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="345" mass="37480" checksum="C0C449F336C3FD79" modified="2024-05-29" version="1">METKFKNNTIYYHRNPTSGTSMEINSEAKWKRPNDVKVQIKALGICGSDVDHFKPMVIGHECAGIIKDIGSNVKSLAVGDRVALEPGISCQTCNFRKNGTYNLCRKMKFFGSPPTNGSLANQVVHPANLVFKLPDSMSLEEGAMCEPLSVGVHACRRAGVNPDTRVLIFGAGPIGLVTMLAARAFGAPRIVTADVDHYRLSIAKDLDADDTVQASTNIQDVGEEVLNIHKVMGESVDISFDCVGFDKTMSTALTATRAGGKVCLVGLGQSQMTIPLVAAAAREVDVIGIFRYRNTWPLCIELLRTGKIDVKPLITHRYKFTQEDVEKAFETSANGGNAIKVMFNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CN44</accession>
    <name>A0AAD5CN44_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_009726</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. KIN-7 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007709">
        <property type="protein sequence ID" value="KAI7743629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CN44"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000779">
        <property type="term" value="C:condensed chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043515">
        <property type="term" value="F:kinetochore binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008608">
        <property type="term" value="P:attachment of spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140694">
        <property type="term" value="P:membraneless organelle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042327">
        <property type="term" value="P:positive regulation of phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901987">
        <property type="term" value="P:regulation of cell cycle phase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033044">
        <property type="term" value="P:regulation of chromosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01374">
        <property type="entry name" value="KISc_CENP_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000026">
        <property type="entry name" value="Centromere-associated protein E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968">
        <property type="entry name" value="CENTROMERE PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968:SF36">
        <property type="entry name" value="KINESIN HEAVY CHAIN ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 8" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6 7 8" id="KW-0505">Motor protein</keyword>
    <keyword evidence="3 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="9">
        <location>
            <begin position="3"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="93"/>
            <end position="100"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7743629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="367" mass="40523" checksum="B6DAD718DBA0F5FE" modified="2024-05-29" version="1">MEKICVAVRVRPPVDLESSTTTTNSNGSHWKVEDNRISLHRSLAGTPLPPALSYTFDHVFDHDCSNSTVYDLLTKDIIHAVLQGFNGTAFAYGQTSSGKTFTMNGSDNNPGIIHRAVKDIFSKTNMSTEREFLIRVSYMEIYNEEINDLFAVENQKLQIHESLERGIFVAGLREEIVNSAEQVLKLIEMGEVNRHFGETNMNARSSRSHTIFRMVIESKGKDSGSNDHSSLDDAIRVSVLNLVDLAGSERIAKTGAGGVRLKEGKHINKSLMTLGNVINKLSDGAKQRGHIPYRDSKLTRILQPALGGNAKTSIICTVAPEEVHIEETKGTLQFASRAKRITNCVQVNEFKAQGALACRLGAERTPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CP38</accession>
    <name>A0AAD5CP38_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Geranylgeranyl diphosphate reductase, chloroplastic</fullName>
            <ecNumber evidence="3">1.3.1.83</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Geranylgeranyl reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_026566</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the reduction of geranylgeranyl diphosphate to phytyl diphosphate, providing phytol for both tocopherol and chlorophyll synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>phytyl diphosphate + 3 NADP(+) = geranylgeranyl diphosphate + 3 NADPH + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:26229"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57533"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:75434"/>
            <dbReference type="EC" id="1.3.1.83"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">Cofactor biosynthesis; tocopherol biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Porphyrin-containing compound metabolism; chlorophyll biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the geranylgeranyl reductase family. ChlP subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.83" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010007550">
        <property type="protein sequence ID" value="KAI7744305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CP38"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102067">
        <property type="term" value="F:geranylgeranyl diphosphate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045550">
        <property type="term" value="F:geranylgeranyl reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015995">
        <property type="term" value="P:chlorophyll biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015979">
        <property type="term" value="P:photosynthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000083">
        <property type="entry name" value="Geranylgeranyl diphosphate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010253">
        <property type="entry name" value="BchP_ChlP_pln/prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011777">
        <property type="entry name" value="Geranylgeranyl_Rdtase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011774">
        <property type="entry name" value="Geranylgeranyl_Rdtase_pln/cyn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050407">
        <property type="entry name" value="Geranylgeranyl_reductase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02023">
        <property type="entry name" value="BchP-ChlP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02028">
        <property type="entry name" value="ChlP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02032">
        <property type="entry name" value="GG-red-SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42685">
        <property type="entry name" value="GERANYLGERANYL DIPHOSPHATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42685:SF4">
        <property type="entry name" value="GERANYLGERANYL DIPHOSPHATE REDUCTASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0149">Chlorophyll biosynthesis</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0602">Photosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding" evidence="13">
        <location>
            <begin position="53"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00033069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00058147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00067953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7744305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="461" mass="51000" checksum="6B4C2D6DE6AD34C4" modified="2024-05-29" version="1">MASITLKSFIGLRQSSLENHHSTITLPSKPLQYLRHNIIASKSSPKITGRNLRVAVIGGGPAGGAAAETLAKGGVETILIERKMDNAKPCGGAIPLCMVGEFDLPLDIIDRRVTKMKMISPSNVAVDIGRTLKPHEYIGMVRREVLDAYLRDRARDAGATIVNGLFVKMDKPFDKYAPYVIHYNAYDGKAGSTGTKMTMEVDAIIGADGANSRVAKSIDAGDYEYAIAFQERIKIPDDKMVYYENLAEMYVGDDVSPDFYGWVFPKCDHVAVGTGTVTHKPDIKKFQLATRLRARDKIQGGKIIRVEAHPIPEHPRPRRLSNRVALVGDAAGYVTKCSGEGIYFAAKSGRMCAEAIVAGSENGTRMVDESDLRNYLQKWDKTYWPTYKVLDILQKVFYRSNPAREAFVEMCADEYVQKMTFDSYLYKTVVPGNPLDDLKLAVNTIGSLVRANALRNEMAKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CPF9</accession>
    <name>A0AAD5CPF9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">BAG family molecular chaperone regulator 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_008231</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007208">
        <property type="protein sequence ID" value="KAI7745532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CPF9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000774">
        <property type="term" value="F:adenyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.120">
        <property type="entry name" value="BAG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039773">
        <property type="entry name" value="BAG_chaperone_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036533">
        <property type="entry name" value="BAG_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003103">
        <property type="entry name" value="BAG_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12329:SF38">
        <property type="entry name" value="BAG FAMILY MOLECULAR CHAPERONE REGULATOR-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12329">
        <property type="entry name" value="BCL2-ASSOCIATED ATHANOGENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02179">
        <property type="entry name" value="BAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00264">
        <property type="entry name" value="BAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63491">
        <property type="entry name" value="BAG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51035">
        <property type="entry name" value="BAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="3">
        <location>
            <begin position="61"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="BAG" evidence="4">
        <location>
            <begin position="154"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7745532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="260" mass="29039" checksum="EF7F146AA1F460EB" modified="2024-05-29" version="1">MLGVKSRNLGIVRTTNRGSATTGGGGGRDVGMAAWDLRPCGMLVQKRNSDVQQSQNIVPIIKVKVKYGASYHEVHIKSRATFGELKKMLAGRTGLNPLDQKLVYKDKERDSTTYLDVAGVTDGSRMLLFIDIPNQEKRLLENIKVAKTDKASMQLLEISSEIDKLVKQVGNLEIEIYGGKKVMEKALLNLIELLMSQLIKLDGIVAEGDVKLQRRMQVKRVQKYIEFLDMLKIRNSNIASNGNQNGVRAPLQRLNKISFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-10-08" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CQ79</accession>
    <name>A0AAD5CQ79_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Terpene synthase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_029440</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007130">
        <property type="protein sequence ID" value="KAI7745799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010333">
        <property type="term" value="F:terpene synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.130">
        <property type="entry name" value="Terpene synthase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001906">
        <property type="entry name" value="Terpene_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036965">
        <property type="entry name" value="Terpene_synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050148">
        <property type="entry name" value="Terpene_synthase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31225">
        <property type="entry name" value="OS04G0344100 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31225:SF252">
        <property type="entry name" value="TERPENE SYNTHASE 12-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01397">
        <property type="entry name" value="Terpene_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Terpene synthase N-terminal" evidence="3">
        <location>
            <begin position="57"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7745799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="165" mass="18980" checksum="DC2134F4135BFFE4" modified="2024-05-29" version="1" fragment="single">XXXXLVFPQSFFDVCKPKLAIQQIAKAECSPHRLICSGAQNKGSQTHRRLAIYQPTIWSHDFIQRLNDDFPANLKEKLRELEKKVMAMNVDIENGSVSTLQLLEHVDDIERLGLGYRFQNHIRRVLDIITSTYEIGIGLEEKEDSLHQASISFRILRQNGYNVSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CW50</accession>
    <name>A0AAD5CW50_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_024601</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006717">
        <property type="protein sequence ID" value="KAI7747606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CW50"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7747606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="53" mass="6045" checksum="E789955AA6210532" modified="2024-05-29" version="1">MYIIVINQSGPSILPPHLILHLHISLFQTLSFTQTLPNQTKSSLPFTIYFFAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D1B3</accession>
    <name>A0AAD5D1B3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Beta-lactamase-related domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_021142</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006169">
        <property type="protein sequence ID" value="KAI7750291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D1B3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="entry name" value="DD-peptidase/beta-lactamase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001466">
        <property type="entry name" value="Beta-lactam-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052907">
        <property type="entry name" value="Beta-lactamase/esterase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43319">
        <property type="entry name" value="BETA-LACTAMASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43319:SF3">
        <property type="entry name" value="BETA-LACTAMASE-RELATED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00144">
        <property type="entry name" value="Beta-lactamase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="beta-lactamase/transpeptidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Beta-lactamase-related" evidence="2">
        <location>
            <begin position="199"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="106"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="623"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="691"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="623"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="660"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="697"/>
            <end position="721"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7750291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="817" mass="90396" checksum="C690CED400BAC872" modified="2024-05-29" version="1">MTLLANVDSTTVSVVVVVRKQGRAENAGVEDNSSKEARAKLHVNLADAKENGRPVDVKEDDLEFRYEKLMRKKSLAIDNVQHEVDPVDALPVKTLDGQVYYKTVSTGSSQSKNAHIDDETVGENEDVGRDNGVVKLTKAEKRAKQKKMRKEAKKVTEVEEGEKSSQAEVLLHRIVDGYMIVQYVNSDIEAKLRSFLLELKNTGRILGIQICAYKDGKVIIDTAAGVLGKDDPRPVQPDNLFPVFSVTKGVTTGMVHWLADKGKLKLDENVANIWPDFGTNGKDQTKVNHVLNHTAGLHNALAGISEHDHALFCDFDECLKRIAMVAPETEPGQVQLYHYLSYGWLCGGIIEHASGKKFQDVLEEAIVRPLDVEGELYIGIPPGVEPRLATVSIDAEDLILLDPEHDIYKSSRYSSPWPSTFTFSMVSSLIPLMNNLNARRAIQPAANGHFSARAVARYYATLVDGGVVPPRHPSQPLSPIQSRPVTDEVDCNIFSNPKEKIHEAFLGSGDYKDLAVTNGIFGMGFIRFKTIDGSPIGVGLPGLGGSTGYCDINNRFAIAVTVNKLSIGALTGEIIRMMRFKFKSCGCVFVLTVHFLLMIPAAKVTDPSKFCRVYESRIRIGQSRSKEGELQPATRDDGSRKKKTIRRRETDQAGNTETLIEDKKPDREKNPRSQPHEEKIFSLPNLSLHHISHSDYKPPPPKTTANPTPPPTSTINSPPPTSVHLNQPHHEVSSVGFSLVQSDAFVIENFVRYEPCGLKILSFFSMGVVLNQSDFSRNLTDVKVLFELGIAFLVFLPTLSFTESQHQTTNLMELLEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D2Y3</accession>
    <name>A0AAD5D2Y3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_020629</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005508">
        <property type="protein sequence ID" value="KAI7753211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D2Y3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36004">
        <property type="entry name" value="AT-RICH INTERACTIVE DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36004:SF1">
        <property type="entry name" value="AT-RICH INTERACTIVE DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="116"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="151"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7753211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="182" mass="19895" checksum="773B9B884CFFBE9C" modified="2024-05-29" version="1">MSLRIVCDTSTITPCTRFPLHRPSLLPPSSSIRTRLHIVSAAKKLSSRTGKFDSKNKRGSTSPGEDEPSSDGAIGTVDSTPEVQNFDGYVLPDLPGLEPDFWEGPQWDAFGFFIEYLWAFGIVFALISSGIAVATYNEGATDFKETPAYKESVQSRDLLEEPEASSPDVFESNPTEEAPSLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D323</accession>
    <name>A0AAD5D323_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">UDP-glycosyltransferases domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_028733</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005659">
        <property type="protein sequence ID" value="KAI7752549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D323"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035251">
        <property type="term" value="F:UDP-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48047">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48047:SF127">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7752549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="307" mass="34462" checksum="CA787FB8CA321796" modified="2024-05-29" version="1">MFNNLKPTPSCIISDNNLSRTANVANMFRIPWVVFDGMSCFTQLVTHNLCASKIHEKLTDFDTFVLPGLSDNISMMKSQLPGLCNLGSSVKGKELKSIRERIREMELGAYGMLINSFDELETRYVDEFKKIKQGRVWCIGPLSNSNKSDLDRAQRGSKIQSTMMFIELALGLEDSKYSFILVMKGGSRTEEIEKWLDEGEFESRVKGRGVLIHGWASQVLILSYRAKGAFLTHCGWNSTIEGVCAGVPMITWPQFAEQSFNERLVVQVLGIGVGVGAQRVMYLEDEDDGVMQVEREDVCKAVMIVMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D492</accession>
    <name>A0AAD5D492_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_031554</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010004801">
        <property type="protein sequence ID" value="KAI7754021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D492"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055296">
        <property type="entry name" value="SRL2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46087:SF11">
        <property type="entry name" value="PROTEIN SEMI-ROLLED LEAF 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46087">
        <property type="entry name" value="PUTATIVE, EXPRESSED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="194"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="207"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7754021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="877" mass="97715" checksum="941AEAD1D053EB02" modified="2024-05-29" version="1">MGVISRKLFPACVNMCVCCPALRSRSRQPVKRYKKLLAEIFPKSPGDSPNERKIAKLAYFAVNLMNVSVELLDDAKRDTVQIIGCQTLVKFIYSQVDGTYTYNLENLVQKLCMLARKPEEEDEKTQLRAASLQCLSAMIWFMTEFSHIFADFDEIVHVILSNYEPNTNAGEDDDRGEAHHNWVDEVVRCEGKGAMNESDSSNMTVKPRTEKKDPSLLTRDEAESPKIWAQICIQRMVELAKESTTMRRILDPMFVHFDTNKQWIPPHGLGLLVLSDMAYFVESPENKQLILASVVHHLDHKNVVHDPELKSHVIQTTTALARQVRSEVALKDIGYVTDLCRHLRKSLQATVESVRDNESLNMLLQSSIEDCLLEIAKGIADSRPLFDVMSTTLENLPPSSIVARATTGSMIILAHMIVVASVSSNAQQVFPEVLLLQLIKLMLHPDVEIRLSGHQIFSVLLIPNSKHLRHDASNQTRRWSSDSASVFAMVTSLLNKLQREKDGSGVEKCEDSIQVEKENAEAERKNVWGHKKSPNFQKISSITAGEMALSNAESSVMKFSEDQITQLLSAFWIQANLPDNTLQNIEALAYSFCLTLISLRLSNPNDNLVVRIFQLPLSLLKTSLDHINGTLCPAHQRSLLTLSTSMLMFAAKMYQISDIADVLKPLLKSDVDNYLGISDDFQVYVKPEADVKEYSSERDNKTAATLLVELQRKMSRSYATIVDILVQKLSNTTQDFPTSSLVEDDAVSESSVSDLSRFITKSPTQSSMSHVISIRQLLESALEAAGQVAGASVSTSPLPFSAMAGQCEALGTDSRKKLSTWLSHANGDTKSADTRCSPNSKVMKILGEDELVRAGMNSMRLPPASPFDNFLKAARYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D4I2</accession>
    <name>A0AAD5D4I2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_012325</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005511">
        <property type="protein sequence ID" value="KAI7753194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D4I2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35713">
        <property type="entry name" value="ARGININE/SERINE-RICH-LIKE SPLICING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35713:SF1">
        <property type="entry name" value="ARGININE_SERINE-RICH-LIKE SPLICING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="107"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="127"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7753194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="136" mass="15638" checksum="551E7FEEDFD7DD06" modified="2024-05-29" version="1">MASMCTSVHSFSPPSVTTTSRLLLLSSSSTSTSVSLFNTRRQRMKTASYSYSMVVCMAPDEEKLTRRNPLDFPVEWERPKPGRRPDIFPQFSPMKTPLPPPLPYDPPPEEEEEEEEEHKEEEEDPDKEQPETRQGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D4Q2</accession>
    <name>A0AAD5D4Q2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CRAL-TRIO domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_018012</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010003968">
        <property type="protein sequence ID" value="KAI7754253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D4Q2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008526">
        <property type="term" value="F:phosphatidylinositol transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.525.10:FF:000008">
        <property type="entry name" value="Phosphatidylinositol transfer protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011074">
        <property type="entry name" value="CRAL/TRIO_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052578">
        <property type="entry name" value="PI_Transfer_CRAL-TRIO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45824:SF24">
        <property type="entry name" value="CRAL-TRIO LIPID BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45824">
        <property type="entry name" value="GH16843P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03765">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01100">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46938">
        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="2">
        <location>
            <begin position="96"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="275"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="280"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="335"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7754253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="345" mass="39382" checksum="0AE37F994267331F" modified="2024-05-29" version="1">MNPQLMTPSLKKTNSNLSVSPQDQPKLINEVRKAIEKHASIDKIKQKLGDEYSRCLSDQSISRYLRARNWNVQKAEKMLEASLIWRMNYKPEEIRWEHVAAEAATGKLYRSSFLDKQGRSVLVMRPRCQNSKSTRAQIKYLVYCMENAIFHLPPEQEQMVWLIDFHGFSMSNISLKTTKETAHVLQDHYPERLGLAILYNPPKFFEPFWKVVKPFLEPKTTSKVKFVYSDDPTTKSYMESLFYIDKLESSFGGRNLAKFDVRTYAIRMKDDDAKRLASHNGETSTSSVSGQASTGSESNHHANTDSEDSDGDKTQFPEINEEEMLAEAHKIATSDGGGTGGRIEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D5Y1</accession>
    <name>A0AAD5D5Y1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_007449</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="JAMZMK010005436">
        <property type="protein sequence ID" value="KAI7753454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D5Y1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13983">
        <property type="entry name" value="STKc_WNK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000075">
        <property type="entry name" value="Probable serine/threonine-protein kinase WNK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000046">
        <property type="entry name" value="probable serine/threonine-protein kinase WNK9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050588">
        <property type="entry name" value="WNK_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13902">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE WNK WITH NO LYSINE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="32"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="463"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="471"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7753454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="578" mass="65250" checksum="BEF03B05D9FF3A2D" modified="2024-05-29" version="1">MLNTKPRSVFGDQDGRSSSNRYVETDPTGRYGRFKEVLGKGAMKTVYKAIDEVLGMEVAWGQVKLNDLLRSPEDLQRLYSEVHLLGTLNHDSIIKFYTSWIDVDRRTFNFITEMFTSGTLKEKKYDRVDIRAIKIWARQILSGLIYLHGHDPPVIHRDLKCDNIFVNGHLGQVKIGDLGLAAILRGSCRAHTVIGTPEFMAPELYEENYNELVDVYSFGMCILEMLTHEYPYSECTNPAQIYKKVTSGKLPRAFYKVKDLEAQCFIGKCLVNASNRLSAKDLMLDPFLAMDDDKLAPAAEYIHQKPFMNDDIEIERLNLNGYPPRTNMSIAGKLNPDDDTIILRVQIADREGSVRNVYFPFDILNDTPCEVAYEMVKELEITDWDPSDISDMIDGEISGLVPNWNNLHQDEADHSHSFHSFSSSSSSQLSFSGARQVDRTTAYGADWLQGTYSNLSYVSAEDHHEKHDLVSPNSRTHNSTRFGQASKNQENMSNVSVDFHKASTSKASTAAGPRLTRNRSLVDMRSQLLHKSLVEEVSKRRQSKTVGTVEDVGFQVPIVGSRSRLGGGQRLRTKGKRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D6D0</accession>
    <name>A0AAD5D6D0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Hyaluronan/mRNA-binding protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_027349</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005311">
        <property type="protein sequence ID" value="KAI7753709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D6D0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039764">
        <property type="entry name" value="HABP4/SERBP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006861">
        <property type="entry name" value="HABP4_PAIRBP1-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019084">
        <property type="entry name" value="STM1-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12299:SF76">
        <property type="entry name" value="HYALURONAN_MRNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12299">
        <property type="entry name" value="HYALURONIC ACID-BINDING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04774">
        <property type="entry name" value="HABP4_PAI-RBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09598">
        <property type="entry name" value="Stm1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01233">
        <property type="entry name" value="HABP4_PAI-RBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hyaluronan/mRNA-binding protein" evidence="4">
        <location>
            <begin position="148"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="27"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="312"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="91"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="107"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="125"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="144"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="181"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="191"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7753709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="359" mass="38943" checksum="A3A5003DB504FAE6" modified="2024-05-29" version="1">MATTNPFDLLIDDDNDDPTQLIAKLPAVAAPLTKKPAAKAAAKLPSKPLPPAQPVQEAKADGQRGGGRFGGRGNGRGGRGGRGFNRDSSENENSYGTNNGFNGGYRPSEDGEVDKSSERRPYSGPRGGYRGGRRGGYTNGDAADGERPRRVFERRSGTGRGNEFKREGAGRGNWGTATDEVAFEPEETVVEVEKNVESEKPAGPEDGVDTNKENVAEEVEEKEPEEKEMTLEEYEKVLEEKRKALQPLKAEERKVALDKDLAKMQLLSNKKSEEEIFVKLGSEKDKRKEAADKEEKAKKSLSINEFLKPAEGERFYSSGGRGRGRGRGSRGGYGGGNRMHDVAAPAIEDVGQFPSLGVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-04-02" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D8U1</accession>
    <name>A0AAD5D8U1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_006715</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000346">
        <property type="protein sequence ID" value="KAI7756556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46978:SF9">
        <property type="entry name" value="TRANSCRIPTION FACTOR INTERACTOR AND REGULATOR CCHC(ZN) FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46978">
        <property type="entry name" value="ZINC KNUCKLE (CCHC-TYPE) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="155"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="196"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="223"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="289"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="303"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="15"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="319"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="344"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7756556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="466" mass="52467" checksum="8B3D2900D7A421AB" modified="2024-05-29" version="1">MGKSARKRPRLPKTNPKSEQDPTSDQPHIILSSDDEADDDLSLKIVQKAMLRACGNEPTTNPSPSVVEVKKKRKKMKEKEKIGVQVDVRKKMKEKEKIGVEVNVVEDVETKEAXXXXMLMQQWTQTLQRNLIILFCESFLGPRYFDPPDNSWGNCYNCGEGGHTAANCTSAKRKKPCFVCGSFDHNVKQCNKGKDCFICKKSGHRAKDCPEKSNFASQSAKICLKCGDSGHEMYSCKGKYSPDDLKEIQCYVCKRFGHLCCVNFAGEGPIEECARVHAETTSNGTPSSCYRCGQEGHMARKCTTSVKKEKKKGESPSFKKNHSKGKHGSHLGVRSVPNHKRNKTQYGEPSSSQPKPRGRGGWITEDPDNHNHNQSHSHHHNNYGWGSPKTPVRYKSNDRFHGSGYKSSNDRFHGSGYNNSNGYHSNQGRFHYDSPVVSNGYQQQRFSASRFGNSTNYGKREYGWDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D8V4</accession>
    <name>A0AAD5D8V4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_009888</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CONSTANS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000341">
        <property type="protein sequence ID" value="KAI7756578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D8V4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009909">
        <property type="term" value="P:regulation of flower development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19821">
        <property type="entry name" value="Bbox1_BBX-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010402">
        <property type="entry name" value="CCT_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045281">
        <property type="entry name" value="CONSTANS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049808">
        <property type="entry name" value="CONSTANS-like_Bbox1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31319">
        <property type="entry name" value="ZINC FINGER PROTEIN CONSTANS-LIKE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31319:SF77">
        <property type="entry name" value="ZINC FINGER PROTEIN CONSTANS-LIKE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06203">
        <property type="entry name" value="CCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51017">
        <property type="entry name" value="CCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="B box-type" evidence="9">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="domain" description="CCT" evidence="10">
        <location>
            <begin position="197"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7756578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="263" mass="29233" checksum="CE4998610F56813C" modified="2024-05-29" version="1">MCEVCEQAPASITCKADAATLCVTCDRDIHSANPLARRHERFPVVPFYDPETEAAKFGGGGEMNDQHYLLDFDVNVAVEAEAASWLLPNPNPNLNPKFNKTADASEVDDLKVTEPDSNYLRSCSVEGLPAYDIDYAGSKPFMYNFTSQSISQTVSSSSLEIDVGLVPGHTGATTDVPNHHHESSSEVYPTPLIGVDREARVLRYKEKRKNRKFEKTIRYASRKAYAETRPRIKGRFAKRMEVDVEAGDVFVSPECLYGVVPTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D9D6</accession>
    <name>A0AAD5D9D6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_003482</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000963">
        <property type="protein sequence ID" value="KAI7755642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D9D6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7755642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="158" mass="17804" checksum="39AB5E209F2CADA6" modified="2024-05-29" version="1">MINKIIRQHGHNSSNNLDGALTTIVKMSSRNVVVKEGFADQFQIPKPLSEAITPEPQVQQEAFWQLRKPHTQKNPPNTTPDNIRTPDSTYKKSDTTTSTSKRSFEEPGPISETKLPFDISTITITAATARVYLRARNLCKRKSTVVAAMRDRRTPSFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-02-05" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DB71</accession>
    <name>A0AAD5DB71_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">UDP-arabinopyranose mutase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_028290</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RGP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000494">
        <property type="protein sequence ID" value="KAI7756255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009505">
        <property type="term" value="C:plant-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052691">
        <property type="term" value="F:UDP-arabinopyranose mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071669">
        <property type="term" value="P:plant-type cell wall organization or biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033356">
        <property type="term" value="P:UDP-L-arabinose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037595">
        <property type="entry name" value="RGP_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31682:SF41">
        <property type="entry name" value="UDP-ARABINOPYRANOSE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31682">
        <property type="entry name" value="UDP-ARABINOSE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03214">
        <property type="entry name" value="RGP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="3" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7756255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="338" mass="38528" checksum="15E53431E144EC0D" modified="2024-05-29" version="1">MAASTFPLKDELDIVIPTIRNLDFLEMWRPFFQPYHLIIVQDGDPSRNIKVPDGFDYELYNRNDINKILGPKASCISFKDSACRCFGYMVSKKKYIFTIDDDCFVAKDPTGKDIDALRQHISNLLNPSTPYFFNTLYDPFTEGADFVRGYPFSLREGVPTAVSHGLWLXXXXTNTARQTSRKEHKGILFPMCGMNLAFDRSLIGPAMYFGLMGDGQPIGRYDDMWAGWCVKVICDHLGLGIKTGLPYIWHSKASNPFVNLKKEYKGIFWQEEIIPFFQDAILPKECITVQSCYKELSKQVKDKLGKIDPYFVKLADAMVTWIDAWDELNPPAKAASSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DBB8</accession>
    <name>A0AAD5DBB8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_031460</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000318">
        <property type="protein sequence ID" value="KAI7756659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DBB8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="86"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7756659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="166" mass="18491" checksum="635E5F8FFE0B8AF2" modified="2024-05-29" version="1">MSFKVLRDTSSDHLVLPPAYVKFHDELQIPFGQPRANMRYCLHALARRSAKTHTWVVLFNTAPPSSSLIHVIDRIPTWIGIVGGKYKPGRKTGSGSFGEIHLATHVDAFEIVAVKIENNTTKHRQLLYEAKLYNLLRGGSGIPSIKWSGVGGEDNILVIDFVRTES</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DDP2</accession>
    <name>A0AAD5DDP2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">GATA-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_019291</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the type IV zinc-finger family. Class A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000053">
        <property type="protein sequence ID" value="KAI7757844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DDP2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00202">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000018">
        <property type="entry name" value="GATA transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051140">
        <property type="entry name" value="GATA_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45658">
        <property type="entry name" value="GATA TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45658:SF153">
        <property type="entry name" value="TRANSCRIPTION FACTOR C2C2-GATA FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00320">
        <property type="entry name" value="GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00401">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00344">
        <property type="entry name" value="GATA_ZN_FINGER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0010">Activator</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GATA-type" evidence="10">
        <location>
            <begin position="199"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7757844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="293" mass="32475" checksum="23961F460C57F004" modified="2024-05-29" version="1">MASQDKSMCIDHGLCDYDDDLGGLLHTFLDFPDESLDADCLTEDLEGSEFGPIPSDVFSEVLPPVGIGGRGGGFADFVTECGGSKLETYQAEFKHIVHKEVSKSQQLATFSQEKKLYRIPKSFEAPSPDSVLERRTSSSARKTVSFGIEIGIPVRTRTKRPRAVSGSGLTGNSWLKSIQQFPLQRVKKKRGKLPQHDDANEVKKCSHCEITKTPQWREGPMGPKTLCNACGVRYRSGRLFPEYRPAASPTFIPSLHSNSHRKVVEMRQKGAAMVNYAMKKPFDELKCSNSTTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DFG0</accession>
    <name>A0AAD5DFG0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">phosphoglycerate mutase (2,3-diphosphoglycerate-independent)</fullName>
            <ecNumber evidence="6">5.4.2.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">M8C21_001634</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">AA19_3_7</strain>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R)-2-phosphoglycerate = (2R)-3-phosphoglycerate</text>
            <dbReference type="Rhea" id="RHEA:15901"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="EC" id="5.4.2.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 3/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the BPG-independent phosphoglycerate mutase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.12" evidence="6"/>
    <dbReference type="EMBL" id="JAMZMK010000071">
        <property type="protein sequence ID" value="KAI7757731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DFG0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004619">
        <property type="term" value="F:phosphoglycerate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006007">
        <property type="term" value="P:glucose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009637">
        <property type="term" value="P:response to blue light"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010037">
        <property type="term" value="P:response to carbon dioxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010118">
        <property type="term" value="P:stomatal movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd16010">
        <property type="entry name" value="iPGM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1450.10:FF:000002">
        <property type="entry name" value="2,3-bisphosphoglycerate-independent phosphoglycerate mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1450.10">
        <property type="entry name" value="BPG-independent phosphoglycerate mutase, domain B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011258">
        <property type="entry name" value="BPG-indep_PGM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006124">
        <property type="entry name" value="Metalloenzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036646">
        <property type="entry name" value="PGAM_B_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005995">
        <property type="entry name" value="Pgm_bpd_ind"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31637">
        <property type="entry name" value="2,3-BISPHOSPHOGLYCERATE-INDEPENDENT PHOSPHOGLYCERATE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31637:SF10">
        <property type="entry name" value="PHOSPHOGLYCERATE MUTASE (2,3-DIPHOSPHOGLYCERATE-INDEPENDENT)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06415">
        <property type="entry name" value="iPGM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01676">
        <property type="entry name" value="Metalloenzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001492">
        <property type="entry name" value="IPGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64158">
        <property type="entry name" value="2,3-Bisphosphoglycerate-independent phosphoglycerate mutase, substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9 12" id="KW-0464">Manganese</keyword>
    <keyword evidence="7 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metalloenzyme" evidence="13">
        <location>
            <begin position="22"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="domain" description="BPG-independent PGAM N-terminal" evidence="14">
        <location>
            <begin position="66"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="134"/>
            <end position="135"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="250"/>
            <end position="253"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="325"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="394"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="398"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="435"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="436"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="465"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR001492-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR001492-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF06415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAI7757731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="522" mass="57236" checksum="9D03D5A6310FA24F" modified="2024-05-29" version="1">MSTTTSTTTWKLADHPKLPKGKTIAVVVLDGWGEASPDQYNCIHVADTPTMDSLKNGAPDRAKLVDLALASGKIYEDEGFNYIKESFANNTLHLIGLMSDGGVHSRLDQLQLLLKGASERGAKRIRVHVLTDGRDVVDGSSVGFAETLEKDLAELRGKGIDAQVASGGGRMYVTMDRYENDWEVVKRGWDAQVLGEAPHKFKNVVEAIKKLREAPNANDQYLPPFVIVDDNGKPVGPIVDGDAVVTFNFRADRMTMLAQALEYEKFDKFDRVRFPKIRYAGMLQYDGELKLPSHYLVSPPLIERTSGEYLVNNGIRTFACSETVKFGHVTFFWNGNRSGYFNKELEEYVEIPSDSGITFNVQPKMKALEIGEKARDAILSRKFDQVRVNIPNGDMVGHTGDIEATIVACKAADQAVKMILDAIEQVGGIYLVTADHGNAEDMVKRNKKGEPLVDKDGKVQIQTSHTLQPVPVAIGGPGLAAGVKFRKDVPNGGLANVAATVMNLHGFVAPDDYETTLIEVVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G3B3</accession>
    <name>A0AAD5G3B3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ribosomal protein L25 beta domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_026945</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011404">
        <property type="protein sequence ID" value="KAI7727284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G3B3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008097">
        <property type="term" value="F:5S rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.120.20:FF:000006">
        <property type="entry name" value="Ribosomal protein L25/Gln-tRNA synthetase, anti-codon-binding domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.120.20">
        <property type="entry name" value="Ribosomal protein L25, beta domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.240.10">
        <property type="entry name" value="Ribosomal Protein L25, Chain P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020056">
        <property type="entry name" value="Rbsml_bL25/Gln-tRNA_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011035">
        <property type="entry name" value="Ribosomal_bL25/Gln-tRNA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020057">
        <property type="entry name" value="Ribosomal_bL25_b-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037121">
        <property type="entry name" value="Ribosomal_bL25_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029751">
        <property type="entry name" value="Ribosomal_L25_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020930">
        <property type="entry name" value="Ribosomal_uL5_bac-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33284">
        <property type="entry name" value="RIBOSOMAL PROTEIN L25/GLN-TRNA SYNTHETASE, ANTI-CODON-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33284:SF1">
        <property type="entry name" value="RIBOSOMAL PROTEIN L25_GLN-TRNA SYNTHETASE, ANTI-CODON-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01386">
        <property type="entry name" value="Ribosomal_L25p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14693">
        <property type="entry name" value="Ribosomal_TL5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50715">
        <property type="entry name" value="Ribosomal protein L25-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Large ribosomal subunit protein bL25 L25" evidence="5">
        <location>
            <begin position="53"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein bL25 beta" evidence="6">
        <location>
            <begin position="164"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF14693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7727284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="257" mass="28485" checksum="7139D5016C3AF9C0" modified="2024-05-29" version="1">MLAPRQVLTRALLFRHIRQSSTAAALVQENDELPLTYLEGFPKPDAKHAETIVAFPRAQSGKNISAKERKAGRVPSIVFEQEDGQHGGNKRLISVQNNQIKKLVNHLGRSYFLSRLFDLEVRPDVDSEDIIEKVRVLPRLLHLHASTDAVLNVTFIRAPSNALLKVDVPLVFIGDDVSPGLKKGSYLNIIKRTVKFICPADIIPPFIEVDLSELDVGQKLVMGDLKVHPALKLVRAKDEPVCKIAGARVSAESKKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G478</accession>
    <name>A0AAD5G478_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_011396</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Fluoride channel required for the rapid expulsion of cytoplasmic fluoride.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>fluoride(in) = fluoride(out)</text>
            <dbReference type="Rhea" id="RHEA:76159"/>
            <dbReference type="ChEBI" id="CHEBI:17051"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:76160"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the fluoride channel Fluc/FEX (TC 1.A.43) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011475">
        <property type="protein sequence ID" value="KAI7726971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G478"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903425">
        <property type="term" value="F:fluoride transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003691">
        <property type="entry name" value="FluC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28259">
        <property type="entry name" value="FLUORIDE EXPORT PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28259:SF1">
        <property type="entry name" value="FLUORIDE EXPORT PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02537">
        <property type="entry name" value="CRCB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="58"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="111"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="144"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="175"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="246"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00035585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7726971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="275" mass="30251" checksum="3A6C753A68C3120F" modified="2024-05-29" version="1">MLKVGSFFMGWFGAVFKGDISNFSPELAVGLTTGYLGSLTTFSGWNQKMLELSVDGHWVFSVLGFLLGLFLVAYSFIFGVETAKGLKWAFNRTTLSSKCGFPAVTSIQSQVMLIMVMVTMLGVLWGVSVTILKKDFDGDSSTSHLWFGCIVGPVGVWVRFYLARFNGQGLGRTHMLKWMPFGTLMANVAAACIMAAFATMKKSVNDRHFDIVATGIQFGFCGCLSTVSTFIAEFEAMRASTHPWRAYVYAFTTMIISFALGTLIFSVPVWTEGWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G9K1</accession>
    <name>A0AAD5G9K1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Nudix hydrolase 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_010794</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010217">
        <property type="protein sequence ID" value="KAI7732446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G9K1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008239">
        <property type="term" value="F:dipeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.540.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039461">
        <property type="entry name" value="Peptidase_M49"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23422">
        <property type="entry name" value="DIPEPTIDYL PEPTIDASE III-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23422:SF9">
        <property type="entry name" value="ZN-DEPENDENT HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03571">
        <property type="entry name" value="Peptidase_M49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7732446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="388" mass="42983" checksum="A84186870CF24234" modified="2024-05-29" version="1" fragment="single">SCLDENEAFLTTADSAIKLVSEAAKPIAGWKGVEYKAAFPLKKPPGANFYPPDMDKTEFELWTKGLSGDEKQDAAGYFSVIKRKSDSGLNNLASSASDLYSVPYSQEYSSLLTKATELLHKAGDLTSSPSLKRLLHSKADAFLSNDYYDSDIVWMELDSKVDVTIGPYETYEDELFGYKATFEAFIGVRDDKATAQVKLFGDHLQVLEQNLPMDSIYKSSEVISAPIRVVQLVYNSGDVKGPQTVAFNLPNDERIVKDRGTSMVMLKNVSEAKFKLILQPIADVCIIKEQQELVDFDSFFTHTICHECCHGIGPHTITLPSGQTSTVRLELQELHSALEEAKADIVGLWALKFLIDKGSLPKSLVKSMYVSFLAGCFRSVRFGLEEAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G9K8</accession>
    <name>A0AAD5G9K8_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_003914</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009754">
        <property type="protein sequence ID" value="KAI7734350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G9K8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7734350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="58" mass="6701" checksum="ACB1492851C936D9" modified="2024-05-29" version="1" fragment="single">MNQKPKPPVQTLTTLAKKKVQEADNQLSIDERNDSLLYVIATKIYHILARHIRRMATV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G9P5</accession>
    <name>A0AAD5G9P5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Enhancer of polycomb-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_014941</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the enhancer of polycomb family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009955">
        <property type="protein sequence ID" value="KAI7733524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G9P5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024943">
        <property type="entry name" value="Enhancer_polycomb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019542">
        <property type="entry name" value="Enhancer_polycomb-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14898">
        <property type="entry name" value="ENHANCER OF POLYCOMB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10513">
        <property type="entry name" value="EPL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Enhancer of polycomb-like N-terminal" evidence="8">
        <location>
            <begin position="369"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="61"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF10513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7733524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="628" mass="71875" checksum="292F985346C0D255" modified="2024-05-29" version="1">MPSSAEMRRTTRVFGARVLRSGRRLLTTQEVFKHMRRPPLAAANVEDDWIELLDHGSGGGEFKENRWRNSDEENERSGKLRKEVKLERSRDVCSVKVVDHDGNSLTENRRWGLVYSRKRARSESIGCGNLDKKFGKKFSRKQSGKNIVTEVSPPSVLVPDIVATEPCLSLKSKSRKKTRVSVTRSTRLNLRSRNGVTNHHDVQKRRSSLRLRKLRNPSSFRNLKSTGGLVKDGVPFFPVKSNPVISKPIRKASIKERKELTQDIDSASCNASILVIESDRCYREVGAVIAMEMVGSKQWFLVVKINGVERFRIEALNIMRPCFCNRVTHAIVWASSDESWRLEFPSRQDWFVFRELYRRCFERTARVQSSLASIIPVPRVKKVSDYADEKYVPFKMPDLYISTGGDEVSRTLEKSDPVYDMDSDDDDWLNKFNDGSDTRVDEDSFEKIIDVFERGIYCSPDDYSDVTEIVDRCSNLASKDVLEAVYGYWIAKRKKRPALVRVFQLYKPKRPDQLNFKSVLRKKRSFRQRGTQAGRGKQLTFLKAALYEKALTVATETEAEAEDTDTKVEETEGAANWAEQAAVMKRQRAQLLMKVADLLTYKAAMAIKIAESRGTSDHTIDELFFTGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GAA1</accession>
    <name>A0AAD5GAA1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_021800</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010125">
        <property type="protein sequence ID" value="KAI7732868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GAA1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18919">
        <property type="entry name" value="bHLH_AtBPE_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000042">
        <property type="entry name" value="transcription factor bHLH48-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024097">
        <property type="entry name" value="bHLH_ZIP_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12565">
        <property type="entry name" value="STEROL REGULATORY ELEMENT-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12565:SF112">
        <property type="entry name" value="TRANSCRIPTION FACTOR BHLH48-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="5">
        <location>
            <begin position="80"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7732868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="230" mass="25569" checksum="59A9D6C3406D4667" modified="2024-05-29" version="1">MEEKREQQACTDTFLLNDSVVPPAVAGNSFTELLELSPNQAVMLLHSPEVKLLAKKRTSVANETNGEELPYVHVRARRGQATDSHSLAERARREKINARMKLLQELVPGCSKISGTAMVLDEIINHVQSLQRQVEFLSMRLAALHPNININLDSIFSAENGSLMDGNLTGTVSPLLSHQIWQQPDGLTPQPLWGVEEMHPNFITPENSLLSYVSSRSSVSLHTNQLKMEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GAW9</accession>
    <name>A0AAD5GAW9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Histone-binding protein RBBP4-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_000537</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat RBAP46/RBAP48/MSI1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009435">
        <property type="protein sequence ID" value="KAI7735775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GAW9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022052">
        <property type="entry name" value="Histone-bd_RBBP4-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050459">
        <property type="entry name" value="WD_repeat_RBAP46/RBAP48/MSI1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22850">
        <property type="entry name" value="WD40 REPEAT FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12265">
        <property type="entry name" value="CAF1C_H4-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Histone-binding protein RBBP4-like N-terminal" evidence="7">
        <location>
            <begin position="16"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="215"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="260"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="309"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="182"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF12265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7735775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="433" mass="47750" checksum="7D72700658FBD630" modified="2024-05-29" version="1">MKDKGGKGGSQASVNERYTQWKSLVPILYDWLANHNLVWPSLSCRWGPLLEQATYKSRQRLYLSEQTDGSVPNTLVIANCEIVKPRVAAAEHIAQFNEEARSPFVKKFKTIIHPGEVNRIRELPQNSKIVATHTDSPDILTGHQENAEFALAMCQSEPFVLSGGKDKSVVLWSIHDHISTLATDSGAGSNGGKSPKSGDDKLPTESPKIQARGIFQGHQDTVEDVQFCPSSAQEFCSVGDDSCLILWDARTGSSPIVKVEKAHNADLHCVDWNPIDENLILTGSADNTVRLFDRRNLTSNGVGSPVHIFTNHNAAVLCVQWSPDKSSIFGSSAEDGVLNIWDHNKIGERSGPASKFAPGLLFRHSGHRDKVVDFHWNSHDPWTIVSVSDDGESTGGGGTLQIWRMIDLIYRPQEEVIDELEKFKSHILTCSTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GE68</accession>
    <name>A0AAD5GE68_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_014119</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009108">
        <property type="protein sequence ID" value="KAI7737141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GE68"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7737141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="128" mass="14299" checksum="A437FE956F00020C" modified="2024-05-29" version="1" fragment="single">MIRCIIHGPQYDDIYNITEMVKSSIGNKTIAFTSSLLEGEMKSGRGTNLTDEKIAATVPLPSPQEIYNKQEIKSGDETDSDSISIFDDMTIQESDLYLREQEEGEGTSKAFAYDDIKSNSPVHDQEIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GED5</accession>
    <name>A0AAD5GED5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_027313</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009119">
        <property type="protein sequence ID" value="KAI7737083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GED5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7737083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="85" mass="10103" checksum="911BE49A2478F7FA" modified="2024-05-29" version="1">MDRFHKEHDDWCRKNKEAKGAKRYKIKQMPSVVGEVEAIASDLKSIMEEEKAKRRQIMQLVARSKFSESFTRVTMVHFYYYQGLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GG20</accession>
    <name>A0AAD5GG20_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Nucleoside diphosphate kinase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">M8C21_019354</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">AA19_3_7</strain>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 2'-deoxyribonucleoside 5'-diphosphate + ATP = a 2'-deoxyribonucleoside 5'-triphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:44640"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:73316"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a ribonucleoside 5'-diphosphate + ATP = a ribonucleoside 5'-triphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:18113"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Cilium</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 11 12">Belongs to the NDK family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008252">
        <property type="protein sequence ID" value="KAI7741175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GG20"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004550">
        <property type="term" value="F:nucleoside diphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006241">
        <property type="term" value="P:CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006183">
        <property type="term" value="P:GTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006228">
        <property type="term" value="P:UTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.141:FF:000010">
        <property type="entry name" value="Nucleoside diphosphate kinase 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.141">
        <property type="entry name" value="Nucleoside diphosphate kinase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034907">
        <property type="entry name" value="NDK-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036850">
        <property type="entry name" value="NDK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001564">
        <property type="entry name" value="Nucleoside_diP_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46161">
        <property type="entry name" value="NUCLEOSIDE DIPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46161:SF3">
        <property type="entry name" value="NUCLEOSIDE DIPHOSPHATE KINASE DDB_G0292928-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00334">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01243">
        <property type="entry name" value="NUCDPKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00562">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54919">
        <property type="entry name" value="Nucleoside diphosphate kinase, NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51374">
        <property type="entry name" value="NDPK_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042030752" description="Nucleoside diphosphate kinase-like domain-containing protein" evidence="13">
        <location>
            <begin position="29"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoside diphosphate kinase-like" evidence="14">
        <location>
            <begin position="28"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="active site" description="Pros-phosphohistidine intermediate" evidence="11">
        <location>
            <position position="145"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAI7741175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="188" mass="20946" checksum="0835E2BA272553A1" modified="2024-05-29" version="1">MHMNLRFGSLCILITIILLPAFRSLAAAELTLAIVKPDGISGNHTNSIKNIILQSGFTIHTQATLHLHQHTVTAFYAEHSTKTFFPSLVKYMTSGPVLIMVLEKVNAIADWRTLIGPTDARKAKITHPDSIRAMCGEDLERNCVHGSDSHESAAREISFFFPDSLSVFMLLLSTGRSNEHEKRSWMND</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GGG2</accession>
    <name>A0AAD5GGG2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">M8C21_017649</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">AA19_3_7</strain>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RLP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008155">
        <property type="protein sequence ID" value="KAI7741640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GGG2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000413">
        <property type="entry name" value="Inactive leucine-rich repeat receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000275">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000095">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000295">
        <property type="entry name" value="probable LRR receptor-like serine/threonine-protein kinase IRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051716">
        <property type="entry name" value="Plant_RL_S/T_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48053">
        <property type="entry name" value="LEUCINE RICH REPEAT FAMILY PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48053:SF22">
        <property type="entry name" value="MDIS1-INTERACTING RECEPTOR LIKE KINASE 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="613"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="642"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAI7741640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="851" mass="93097" checksum="AAAC4718EE039CD6" modified="2024-05-29" version="1" fragment="single">IGHDDNINNDDDVIGLIAFKADIIDPSSHLSSWNQDDISPCSWKFITCNPATGRVIDLSLIGLNLSGKIGRGLEKVQNLQVLNLAHNNFTGSLNGLSPQLSVLTNLENLNLSRNGLSGGVPPFLTNSRKLKFLDLSKNLLSGPVPDEFFLTCSSLRLLSLSGNNLNGPIPNSVSQCTSLNHLNLSKNRFSGSPDFSKSLWLLSRIRTLDLSHNALTGHVPDGVVSLHYLKELFLQGNHFTGESVPVSFQGLSCLTYLNLGNNVLTGDFPKWIGRLNSLEYLDVSGNGLTGTVPESVGGLGSLTYMSLSGNNISGTIPKSLIYSSKLSVVRLRGNRFNGSIPEGLFELGLNQIDLSRNQLTGSIPPGSSRLFETLESMDLSANGLTGDIPAEMGLLSKLRYLNLSWNNFETRMPLKLGYFENLTVLDLRNGAFHGSLPADICNSGSLEILQLDGNALTGNIPEEIGKCSSLYLLSMSYNDFRGFIPRSMSLLKKLKILKLDSNQLSGEIPQELGGLENLLAVNISYNRLQGRLPDRGIFPSLQESSLEAVLISIGVLVISLLNISARKRLQFVSNALESCSSSSRSTRSLSIGKLVWFDSKIAPGWVANPESLLHKAAEIGKGVFGTVYKASLGEEGRDLAIKNLVVSNMVQHPEDFDREVRVLGKARHPNLVSVKGYYWTPNLQLLVTDYAPNGSLQSKLHEWSTSSQPLSWPKRIVAKLDKKVMSNRFQSALGYVAPELACQSLRVNEKCDVYGFGVLILETVTGRRPIEYGDDNVLILEEQVKMMLEEGNVLECVDESMGEYPEEEVLPVVKLALVCTSQIPSSRPSMAEVIQILQVIQTPIPHRMDAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GGY1</accession>
    <name>A0AAD5GGY1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">M8C21_028024</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">AA19_3_7</strain>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010008533">
        <property type="protein sequence ID" value="KAI7740006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GGY1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009742">
        <property type="term" value="P:brassinosteroid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000016">
        <property type="entry name" value="probable serine/threonine-protein kinase At4g35230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000154">
        <property type="entry name" value="probable serine/threonine-protein kinase At4g35230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000069">
        <property type="entry name" value="probable serine/threonine-protein kinase At5g41260"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045845">
        <property type="entry name" value="BSK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058209">
        <property type="entry name" value="TPR_BSK1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45863">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE BSK5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45863:SF51">
        <property type="entry name" value="TRANSFERASE, PROTEIN KINASE RLK-PELLE-RLCK-XII-1 FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25575">
        <property type="entry name" value="TPR_BSK1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1070">Brassinosteroid signaling pathway</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0519">Myristate</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="30"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="17">
        <location>
            <position position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAI7740006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="452" mass="50486" checksum="C3D9C5D6B55E9B7B" modified="2024-05-29" version="1" fragment="single">NELGEASDLPAFREFSIEQLRTATSGFAVENIVSEHGEKAPNVVYKGKLANQRRIAVKRFNRSAWPDSRQFLEEARAVGQLRNPRLANLLGCCCEGDERLLVAEFMPHETLAKHLFHCKGNTTYAMGNAVTEALEYCTSRGRALYHDLNAYRVVFDDDANPRLSCFGLMKNSRDGKSYSTNLAFTPPEYLRTGRVTPESVMYSFGTLLLDLLSGKHIPPSHALDLIRDRNLQMLTDSCLEGQFSTDDGTELVRLASRCLQYEPRERPNPRSLVSALVPLQKETEVPSHVLMGIPQGSTMPLTPLGEACLRMDLTFIHDVLEKLGYKDDEGAATELSFGMWTNQIHDTLNSKKKGDSAFRNKEFKAAIDLYTQFIEVGTMVSPTVFARRSLSHLMSDMPQEALNDAVQAQVISPIWHIASYLQAAALFALGRENEAQIALREGTVLEEKHNDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GH07</accession>
    <name>A0AAD5GH07_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Short-chain dehydrogenase TIC 32, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_012005</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008019">
        <property type="protein sequence ID" value="KAI7742315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GH07"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05327">
        <property type="entry name" value="retinol-DH_like_SDR_c_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055280">
        <property type="entry name" value="TIC32"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48476">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE TIC 32, CHLOROPLASTIC-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48476:SF3">
        <property type="entry name" value="VERY-LONG-CHAIN 3-OXOACYL-COA REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7742315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="312" mass="33743" checksum="C41955C3C4666D06" modified="2024-05-29" version="1">MTWISIFTGKSGFAASSTAEDVTKGIDGTGLTAIVTGATSGIGLETARVLALRGVNVVMPVRNIESGEKVKQSIAEKIPNAKLEVMELDVSSLESVRKFASQYCSKGLPLNILILNAGIMTPPFTLSKDNIELQFATNHVGNFLLTNLLLDTMKKTAKESGKEGRIVILSSEVHRVTYKGGIMFDKLNDEKSYNALYAYGQSKLANALHAYELTRRLQEEGVNITANSLHPGVIKTNLARHGAVFVNAVQSFLKNVPQGAATTCYVALHPQLKGVSGQYFADSNLSKASGYARDPELAKKLWDFSVKLTESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GHZ4</accession>
    <name>A0AAD5GHZ4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_033048</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008323">
        <property type="protein sequence ID" value="KAI7740921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GHZ4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47447">
        <property type="entry name" value="OS03G0856100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47447:SF23">
        <property type="entry name" value="PENTACOTRIPEPTIDE-REPEAT REGION OF PRORP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13812">
        <property type="entry name" value="PPR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="11"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="166"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="229"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="264"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="299"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="339"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="374"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="409"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="444"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="479"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="514"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="549"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="29"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7740921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="612" mass="68601" checksum="790F0B34D8310F8C" modified="2024-05-29" version="1">MNTLLLPPPSSTTTRFLHHRNTATPPPSTTSYKPTPPHSSLTSLSLSQDDVITPPHDDDDVVSPLSTRRFDFSPLLHFLSTDSSQLTESELVESYKSVPPTLWHYLIKTLSSSPDSVTTTHALVDWLHNHNLCSSHEFLYSTLIHALSRAEIMYEAFLLSQTQTLTPLTYNVLIGACARNDDLEKAVILLNRMRAEGHLYGIIILILAGDVDRAMYFLGLIQGNGLTAKVSTVVAIVSLLGSLGRVEEAEAVFEEIKDGGLRPRTRAYNAVLRGYVVKGGLRDAEWVVEEMERNGVTADEYTYSLLIEAYGDAGRWESARIVLKEMEANNLKACGVKPDRQFYNVMIDTFGKYNCVDNALAVLERMRGEGIEPDNVTWNTLIDCHCKAGQHDKAEELFDEMQQRGCSPCVATYNIMINSFGEQERWEGVRGLLRKMKSQGLQPNVITYTTLVDIYGRSGRFMDATECLEDMKSAGLKPTSTIYNALINAYAQRALSDDALNAFRVMRSDGLKPSNLALNALINAFCEDKRDVEAFAILRSMKENDLKPDVVTYTTLMKALIRVEKFDEVPAVYEEMILSGCTPDRKARAKLRSALKYMRQRLKSEFHTTAKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GJG4</accession>
    <name>A0AAD5GJG4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF1421 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_019372</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007361">
        <property type="protein sequence ID" value="KAI7745105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GJG4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010820">
        <property type="entry name" value="DUF1421"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31805:SF16">
        <property type="entry name" value="FORMIN-LIKE PROTEIN (DUF1421)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31805">
        <property type="entry name" value="RECEPTOR-LIKE KINASE, PUTATIVE (DUF1421)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07223">
        <property type="entry name" value="DUF1421"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF1421" evidence="2">
        <location>
            <begin position="437"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="166"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="393"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="169"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="234"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="251"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="264"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="305"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="346"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="364"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="411"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7745105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="488" mass="53293" checksum="BF56788A3A51D8C3" modified="2024-05-29" version="1">MASGSSKGFDFASDDILCSYEDYTNQEQSNGSHQDPAIATNPTKEFNKSRMARTSVFPNSSYSPPDDSGNQEVIIMAVERSMKKHTDNVMRFLEGLSSRLSQLELYCYNLDKSIGEMRGDLTRDHGEAETKLKFLEKHLQEVHRSVQILRDKQELADTQKELAKLQLAQKESSSSSSQQNEEGSSTPDGRKKESSPDSHGQQLALVPAPAPQPQRTPEPVAQPQGYYLPPAPLQAPAQVNQTQYPQAQMPPTQPQQQPPPPPQVHQVPQYQQQWGPQVPPQPGMQPGSRPASPQVYPQYLMTQPPVNPSPPEPAPNSMPMQMPYSGVPPPGSSQPVNYSYGGPGPVRTPQPPPQQPPPQHMKPGYGGQPGDGYAAGGAPNAYMMYDNEPGRVQHHNQGGQFYPPNQPQQSGGIGGGGGGGGGMMSRPAQQYMHNHPYNELIDKLVSMGYRGDHVMSVIQRLDESGQTVDFNAVLDKLNGHPSQRGWSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GKK1</accession>
    <name>A0AAD5GKK1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PEP-utilising enzyme C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_030588</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006994">
        <property type="protein sequence ID" value="KAI7746337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GKK1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050242">
        <property type="term" value="F:pyruvate, phosphate dikinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="entry name" value="Phosphoenolpyruvate-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000121">
        <property type="entry name" value="PEP_util_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010121">
        <property type="entry name" value="Pyruvate_phosphate_dikinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22931">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE DIKINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22931:SF9">
        <property type="entry name" value="PYRUVATE, PHOSPHATE DIKINASE 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02896">
        <property type="entry name" value="PEP-utilizers_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PEP-utilising enzyme C-terminal" evidence="1">
        <location>
            <begin position="3"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7746337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="48" mass="4954" checksum="8DB03EC84DB12A7A" modified="2024-05-29" version="1">MDTQVGICGEHGGEPSSVAFFDGVGLDYVSCSPFRVPIARLAAAQVVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GLL0</accession>
    <name>A0AAD5GLL0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_020351</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007606">
        <property type="protein sequence ID" value="KAI7744043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GLL0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015184">
        <property type="term" value="F:L-cystine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005282">
        <property type="entry name" value="LC_transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006603">
        <property type="entry name" value="PQ-loop_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13131">
        <property type="entry name" value="CYSTINOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13131:SF5">
        <property type="entry name" value="CYSTINOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04193">
        <property type="entry name" value="PQ-loop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7744043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="142" mass="15838" checksum="0323CAB523A6B58B" modified="2024-05-29" version="1" fragment="single">TLNHTIKPPSHSLTGQRSFTFSLLLARRERDREIDRERRIQIGSLERGNIGRQLEGHNQGEGHGVPFPLIIHQMISIAANDVAFSSHGVLLTAFTLFQIAIYDAIMNFKRKSIIGFSIGNILLDLLGGLTNYGQMVVQSIDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GMF8</accession>
    <name>A0AAD5GMF8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tryptophan synthase</fullName>
            <ecNumber evidence="3">4.2.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_000012</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine = D-glyceraldehyde 3-phosphate + L-tryptophan + H2O</text>
            <dbReference type="Rhea" id="RHEA:10532"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:58866"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.2.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 5/5.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.20" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010007378">
        <property type="protein sequence ID" value="KAI7745033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GMF8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004834">
        <property type="term" value="F:tryptophan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000004">
        <property type="entry name" value="Tryptophan synthase beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023026">
        <property type="entry name" value="Trp_synth_beta/beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48077:SF4">
        <property type="entry name" value="TRYPTOPHAN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48077">
        <property type="entry name" value="TRYPTOPHAN SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001413">
        <property type="entry name" value="Trp_syn_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="10">
        <location>
            <begin position="108"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7745033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="380" mass="41185" checksum="B4DBDE9308C4E948" modified="2024-05-29" version="1" fragment="single">KKRISHTISTVSGEKNPIVGKFGSFGGKFVGEVLISPLAELESEFNSVLLDHDFKMEVETALRDYVGRETPLYHAQKLTDHYKNINGEGPEIYIKREDLSHGGSYKMNNVVAQAILAKRMGRKSIITATNMLRKPSNVQLMELLGAQVKPVEGSFQAAASESTREWISNLQTGYYLAGTAVGPHPIPTMVREFNSVIGKETRRQAMEKWGGSPDVLVACVGTGCNALGLFHEFVGDKNVRMIGVEGGGVGTNTGDQLHSASLVRGEVGVYHGAMCYLLQDEQGQITKTQSIASGLEFPGVSPELSFLKDAGRIECYTVTDQEALDAYKRVCRLEGIVPALEASHAFAYLEKLCPKLPHGAKVVVNCSGTGYNDATTVLNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GN11</accession>
    <name>A0AAD5GN11_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Xyloglucan endotransglucosylase/hydrolase</fullName>
            <ecNumber evidence="6">2.4.1.207</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_033894</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Secreted</location>
            <location evidence="6">Cell wall</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Secreted</location>
            <location evidence="6">Extracellular space</location>
            <location evidence="6">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="6">Contains at least one intrachain disulfide bond essential for its enzymatic activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the glycosyl hydrolase 16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.207" evidence="6"/>
    <dbReference type="EMBL" id="JAMZMK010006419">
        <property type="protein sequence ID" value="KAI7748977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GN11"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016762">
        <property type="term" value="F:xyloglucan:xyloglucosyl transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042546">
        <property type="term" value="P:cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010411">
        <property type="term" value="P:xyloglucan metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02176">
        <property type="entry name" value="GH16_XET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000025">
        <property type="entry name" value="Xyloglucan endotransglucosylase/hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000757">
        <property type="entry name" value="Beta-glucanase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044791">
        <property type="entry name" value="Beta-glucanase/XTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010713">
        <property type="entry name" value="XET_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016455">
        <property type="entry name" value="XTH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31062">
        <property type="entry name" value="XYLOGLUCAN ENDOTRANSGLUCOSYLASE/HYDROLASE PROTEIN 8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00722">
        <property type="entry name" value="Glyco_hydro_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06955">
        <property type="entry name" value="XET_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005604">
        <property type="entry name" value="XET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51762">
        <property type="entry name" value="GH16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0052">Apoplast</keyword>
    <keyword evidence="6" id="KW-0134">Cell wall</keyword>
    <keyword evidence="6" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="1 6" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="22"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="GH16" evidence="9">
        <location>
            <begin position="14"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="322"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="330"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="126"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR005604-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7748977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="346" mass="39276" checksum="C03C8C02A1639C8D" modified="2024-05-29" version="1">MYALVGCTLGSKTSTHKNKMQTFHLMLALLCSVFVLGHGFSRNLPILSFDEGYSHLFGDNNLIVLKDGKSVHLSLDQRTGSGFVSQDLYLHGFFSASIKLPADYTAGVVVAFYMSNGDIFEKNHDEIDFEFLGNIRGKEWRIQTNIYGNGSTKVGREERYGLWFDPTDDFHQYSILWTHTHIIFYVDDVPIREIKRTESMGGDFPSKPMTLYATIWDASEWATNGGKYKVNYKYAPYIAEFSNFVLHGCTADPIELTSLKCEDASNAKSIPTGITPAQRAKMVSFRKKHMQYSYCYDKIRYKAPPSECVINQKEAERLKKFDPVTFGGGRGHHHGKRHRRSQAVAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GNI6</accession>
    <name>A0AAD5GNI6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_016706</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006739">
        <property type="protein sequence ID" value="KAI7747504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GNI6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7747504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="67" mass="7986" checksum="DCE95F0E99A0975D" modified="2024-05-29" version="1" fragment="single">CKQQKSGWECGYLVIQHMFEFLHFYVDQFPIRMWNNTSYATDKDIDLMLKTLVPTFFSELGIRIFSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GNS6</accession>
    <name>A0AAD5GNS6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PGG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_009324</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006687">
        <property type="protein sequence ID" value="KAI7747789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GNS6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47303">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47303:SF1">
        <property type="entry name" value="NF-KAPPA-B INHIBITOR BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="232"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="263"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="46"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="82"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7747789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="297" mass="33356" checksum="E2C7E55BFC0C5B09" modified="2024-05-29" version="1">MEFMRLVDEQVNRNELHSVTKQGDWPKAESILGKNKVVLTEALRSDGSTILHIAVGIGHNNFVKQLLSYMNDEQVVQQRDSDGSTTLHIAAIVGNTDIADLLVKRNRMLLRVKGYKGVEPLQKAYEYMHLDTIGYLLKSVNDDGKTELQSSLTRSVQPDDDIAVNLLVNAISAKQYSLALELVQNFPKSASRSDDVLMALAKNFPSGLDHWEKLVYPSPLSWLDNIRLKVEALTIVLFLGFPVFVLYKFIVKGSPSDKGIATLLFKSVILTVQAWLALPFVFTNVNQRMILILVFKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GRA1</accession>
    <name>A0AAD5GRA1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">LIM zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_030134</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006321">
        <property type="protein sequence ID" value="KAI7749621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GRA1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051017">
        <property type="term" value="P:actin filament bundle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000002">
        <property type="entry name" value="LIM domain and actin-binding 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24206">
        <property type="entry name" value="OS06G0237300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0440">LIM domain</keyword>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="6">
        <location>
            <begin position="59"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="126"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="126"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7749621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="155" mass="17392" checksum="53307A0DC134C14F" modified="2024-05-29" version="1">MSNYSSFEGVLYCQPHFNQLFKMTGSLDKSFEGTPRTARSYEQGQSNSKVSSMFGGTQEKCVSCKKTVYPLEKVGVDGNAYHKACFRCSYGGCHISSSNYVTHEQKLYCKHHHAQLFMAKGDFSQFDKRHEPEKGKQHEPENGVPNGVTESTTEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GRL1</accession>
    <name>A0AAD5GRL1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Fiber protein Fb34</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_033060</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the DESIGUAL family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006355">
        <property type="protein sequence ID" value="KAI7749473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GRL1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009606">
        <property type="entry name" value="DEAL/Modifying_wall_lignin1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052222">
        <property type="entry name" value="DESIGUAL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31769">
        <property type="entry name" value="OS07G0462200 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06749">
        <property type="entry name" value="DUF1218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="55"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="96"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="138"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7749473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="188" mass="20520" checksum="A9430D0FE2C5F379" modified="2024-05-29" version="1">MGERKGSSLVHLLVIILCLLAFAFSIAAERRRSSGTIRTDDRTNSTYCVYDSDVATGYGVGAFLFLLSGQSLLMGVTKCMCFGRPLAPGGNRAWTIIYFTFSWLCFVVAEACLIAGAKKNAYHTKYKSMTESFSCDTIRKGVFVAGAAFVVATMILNVYYYMYFTKATSQPAHKTNRSGSTVGMAAYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GRY3</accession>
    <name>A0AAD5GRY3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">OTU domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_016812</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C85 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005560">
        <property type="protein sequence ID" value="KAI7753057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GRY3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003323">
        <property type="entry name" value="OTU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050704">
        <property type="entry name" value="Peptidase_C85-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12419">
        <property type="entry name" value="OTU DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12419:SF108">
        <property type="entry name" value="UBIQUITIN THIOESTERASE OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02338">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50802">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OTU" evidence="2">
        <location>
            <begin position="85"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7753057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="238" mass="26578" checksum="92EA9CE99D0BEDF1" modified="2024-05-29" version="1" fragment="single">SVWHAILPAGNSRGKYNLAGEGSWNVAWDVRPARWLHRPYTAWLLFGICACLAAPPVEVTDTGLEPVVVDDNNKVNGNIIPTVCESSTGVPADGRCLFRAIAHMVCLRNGQGAPDENQQKELADKLRAQSTIMNLISIFEVVDELLKRRKEIEWFIEEDFDVYIKRIEKPYVWGGEPELLMASHVLKTMISVFMLEGSTKKLLNIATYGKEYEKDDKSSIKIPLHSSHANISLNGSKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GS81</accession>
    <name>A0AAD5GS81_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_002110</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006196">
        <property type="protein sequence ID" value="KAI7750148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GS81"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7750148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="35" mass="3837" checksum="4BAA579538791094" modified="2024-05-29" version="1">MVGQPTVTIKPSRSDDTSQSPLRFYSAKTSPQTQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GSZ0</accession>
    <name>A0AAD5GSZ0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">WRKY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_029102</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005009">
        <property type="protein sequence ID" value="KAI7753970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GSZ0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.80">
        <property type="entry name" value="WRKY domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003657">
        <property type="entry name" value="WRKY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036576">
        <property type="entry name" value="WRKY_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044810">
        <property type="entry name" value="WRKY_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31282">
        <property type="entry name" value="WRKY TRANSCRIPTION FACTOR 21-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03106">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00774">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118290">
        <property type="entry name" value="WRKY DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50811">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="WRKY" evidence="7">
        <location>
            <begin position="169"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="73"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="77"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7753970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="248" mass="27247" checksum="DB2BAC43DDA48849" modified="2024-05-29" version="1">MAVDYIGVQQVDHHLNNNMIIMSSQDYNVSTGYKRILFTTTKRTGHARFRRAPSSQFEDNKVPVSSNEQKEFFLNKSVDTSSMSTSSSSLSSGGGELEGSVSNGKRLGVVAPAPAFSSRKPPLPATHRKRCVADRPVVANVHGSGSGCCKRRKTGSKREIRRVPIIGSKVTSIPADDYSWKKYGEKRIEGSPYPRVYYKCSTEKGCPATKRVELATDNSKMLLVTYDGEHRHRLVPKPVPTGLTGLAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GTC7</accession>
    <name>A0AAD5GTC7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 15">Glucose-1-phosphate adenylyltransferase</fullName>
            <ecNumber evidence="7 15">2.7.7.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">ADP-glucose pyrophosphorylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">M8C21_015685</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">AA19_3_7</strain>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 15">This protein plays a role in synthesis of starch. It catalyzes the synthesis of the activated glycosyl donor, ADP-glucose from Glc-1-P and ATP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>alpha-D-glucose 1-phosphate + ATP + H(+) = ADP-alpha-D-glucose + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:12120"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57498"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="EC" id="2.7.7.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4 15">Glycan biosynthesis; starch biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6 15">Heterotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 15">Plastid</location>
            <location evidence="3 15">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 15">Belongs to the bacterial/plant glucose-1-phosphate adenylyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.27" evidence="7 15"/>
    <dbReference type="EMBL" id="JAMZMK010005827">
        <property type="protein sequence ID" value="KAI7751736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GTC7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008878">
        <property type="term" value="F:glucose-1-phosphate adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005978">
        <property type="term" value="P:glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019252">
        <property type="term" value="P:starch biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02508">
        <property type="entry name" value="ADP_Glucose_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000030">
        <property type="entry name" value="Glucose-1-phosphate adenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011831">
        <property type="entry name" value="ADP-Glc_PPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005836">
        <property type="entry name" value="ADP_Glu_pyroP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02091">
        <property type="entry name" value="glgC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002772">
        <property type="entry name" value="PRK02862.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43523:SF12">
        <property type="entry name" value="GLUCOSE-1-PHOSPHATE ADENYLYLTRANSFERASE LARGE SUBUNIT 1, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43523">
        <property type="entry name" value="GLUCOSE-1-PHOSPHATE ADENYLYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25247">
        <property type="entry name" value="LbH_GLGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="Trimeric LpxA-like enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00808">
        <property type="entry name" value="ADP_GLC_PYROPHOSPH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00809">
        <property type="entry name" value="ADP_GLC_PYROPHOSPH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00810">
        <property type="entry name" value="ADP_GLC_PYROPHOSPH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="13 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 15" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="12 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11 15" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="15" id="KW-0934">Plastid</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14 15" id="KW-0750">Starch biosynthesis</keyword>
    <keyword evidence="10 15" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleotidyl transferase" evidence="16">
        <location>
            <begin position="86"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU362093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAI7751736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="460" mass="51542" checksum="AF703E83DF177FE0" modified="2024-05-29" version="1">MDSCCLTFNPHMHVGKRSKFVENGFLGKKINSFDSHFCKISRNGSKRLKKGAVVRSVLKSPVDEELLTFERQYYDEKKVEAKTVASIILGGGAGTRLFPLTSKRAKPAVPIGGCYRLIDVPMSNCINSGIRKIFILTQFNSFSLNRHLAKTYNFGNGMSFGDGFVEVLAATQTPGEAGKKWFQGTADAVRQFIWLFEDAKNKNVENILILSGDHLYRMDYMDFVQKHVDTNADITVSCIPMDDSRASDYGLMKIDDTGRIVHFAEKPKGNLLKSMQVDTTLLGLSDHEAMKNPYIASMGVYVFKTDVLLELLKWKYPSCNDFGSEIIPYAVSEHNVQAYLFKDYWEDIGTIKSFFDANLALTEQPPKFDFNDPKTPFFTSPRFLPPTKVERCRIIDAIISHGCFLRECSVEHSVVGIRSRLDQGVELKDTMMMGADYYQTDSEIAAMVATGRVPVGSGTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GUU8</accession>
    <name>A0AAD5GUU8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_020281</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005523">
        <property type="protein sequence ID" value="KAI7753176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GUU8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14703">
        <property type="entry name" value="bZIP_plant_RF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044759">
        <property type="entry name" value="bZIP_RF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13690">
        <property type="entry name" value="TRANSCRIPTION FACTOR POSF21-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13690:SF86">
        <property type="entry name" value="TRANSCRIPTION FACTOR VIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="8">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="92"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="19"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="98"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="119"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7753176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="152" mass="17197" checksum="37E3A3C8457B5C39" modified="2024-05-29" version="1" fragment="single">ILANRQSAARSKERKMRYTSELEKKVKTLQTEATTLSAQVTKLQRDTSDITTENKELKLRLEAMEQHAQLRDALNEALREEVNRLKLEAGQPPLLNGMTYSASLPPQYSSRPQPLHHFANLNGQQAQPQPQNTQTPNSNNAGQLKPSFMDFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GVJ6</accession>
    <name>A0AAD5GVJ6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">M8C21_019198</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">AA19_3_7</strain>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the splicing factor SR family. RSZ subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000140">
        <property type="protein sequence ID" value="KAI7757287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GVJ6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd12373">
        <property type="entry name" value="RRM_SRSF3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000214">
        <property type="entry name" value="Serine/arginine-rich splicing factor 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050907">
        <property type="entry name" value="SRSF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23147">
        <property type="entry name" value="SERINE/ARGININE RICH SPLICING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RRM" evidence="13">
        <location>
            <begin position="2"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="14">
        <location>
            <begin position="98"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="66"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="112"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="74"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="125"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00061011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAI7757287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="224" mass="24746" checksum="86661085E8555E8D" modified="2024-05-29" version="1">MSRVYVGNLDPRVTERELEDEFRSYGVLRNVWVARRPPGYAFVEFDDRRDALDAIRALDGKNGWRVELSHNSKGDGGGRGGRGGGGDRGGRGGGDDLKCYQCGEPGHFARECRNRGGSRGFGGGGRRRSPSPRRRRSPIATALATVLVVGGHHHLGVAYHLAEAAATAGRLPRTAVPDVFPHMLMESKMEIIRDGWRLLNVRSSPEKRPFVVFYFLHYTASRGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GXJ0</accession>
    <name>A0AAD5GXJ0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_023614</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000768">
        <property type="protein sequence ID" value="KAI7755846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GXJ0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38390">
        <property type="entry name" value="OS01G0103900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38390:SF2">
        <property type="entry name" value="OS01G0103900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="286"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="428"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="296"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="428"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7755846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="452" mass="50372" checksum="C58492318C9CD739" modified="2024-05-29" version="1">MVMLCFLLDLRTLSPPLLRDLKQSLLQLANFYAISSPGGHRSVSKSKSLRDKIGICYVVRNRVSCSTEMKVAYRPSGNFSLRDFHHAVNNLPTDAFLPELNNSIAIPCSDAHLETVLSDKVIYSWGGDNEDVGRKVIVISSCLLENLDSVAKKALTDAADKCVSVDFVLLEQVSNGAGSLSESINNFVKNICDLENCSFRNHLPEWLNDLRDEAEELQGRLKFKRNIVGSLNQICCNLIPSFNPIIDEFEPCKIEVRDYNPIPHERGFHQKLNVLVKESLHFGSLPSKSEELPHEPTSSQLDTLNESGPLKQSTGMLALKEEIPPLDLKSGQDKVTARITEEWEQLVVNEIPNMKSPTSVSKPKLDNLVVLPLESNKPLDEKTSRILERLEVPRKLKAKVASPVITSSSAPTDACMSTKKPLIPFQPTQNSVIGTVPSQPMKPNFQRLKRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BJV0</accession>
    <name>A0AAD5BJV0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">tRNA/rRNA methyltransferase SpoU type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_009258</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012216">
        <property type="protein sequence ID" value="KAI7724470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BJV0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18096">
        <property type="entry name" value="SpoU-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051259">
        <property type="entry name" value="rRNA_Methyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001537">
        <property type="entry name" value="SpoU_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43191:SF7">
        <property type="entry name" value="OBP33PEP LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43191">
        <property type="entry name" value="RRNA METHYLTRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00588">
        <property type="entry name" value="SpoU_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="alpha/beta knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="tRNA/rRNA methyltransferase SpoU type" evidence="4">
        <location>
            <begin position="50"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="29"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7724470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="262" mass="28991" checksum="818FFAADCF41FAA6" modified="2024-05-29" version="1">MSQQNHLQNQSTEQGLGFSLPNTMEDDNQTQTQTPNPNQNQNNNEGNKSYVVIHNIAKRHNVGTLARSATAFGVSELILVGRRDFNSFGSHGSTSHLPFRHFYSLSDARLFLKEKDIDICGVEITDDATAVSSHPFRKSTAFLLGNEGSGLSAKECEFCDYFVYIPQYGCGTASLNVTVAASIILHHFAVWAGFPERTRNGNKFVVAERPVHKVGRNFCTETAESVTEERKLKRENDAKNSFFEDMGGSESSNNLLDAVFGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BK68</accession>
    <name>A0AAD5BK68_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Xyloglucan endotransglucosylase/hydrolase</fullName>
            <ecNumber evidence="5">2.4.1.207</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_004602</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Secreted</location>
            <location evidence="5">Cell wall</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Secreted</location>
            <location evidence="5">Extracellular space</location>
            <location evidence="5">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="5">Contains at least one intrachain disulfide bond essential for its enzymatic activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the glycosyl hydrolase 16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.207" evidence="5"/>
    <dbReference type="EMBL" id="JAMZMK010012171">
        <property type="protein sequence ID" value="KAI7724725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BK68"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016762">
        <property type="term" value="F:xyloglucan:xyloglucosyl transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042546">
        <property type="term" value="P:cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010411">
        <property type="term" value="P:xyloglucan metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000757">
        <property type="entry name" value="Beta-glucanase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044791">
        <property type="entry name" value="Beta-glucanase/XTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010713">
        <property type="entry name" value="XET_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016455">
        <property type="entry name" value="XTH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31062">
        <property type="entry name" value="XYLOGLUCAN ENDOTRANSGLUCOSYLASE/HYDROLASE PROTEIN 8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00722">
        <property type="entry name" value="Glyco_hydro_16"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06955">
        <property type="entry name" value="XET_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005604">
        <property type="entry name" value="XET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51762">
        <property type="entry name" value="GH16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0052">Apoplast</keyword>
    <keyword evidence="5" id="KW-0134">Cell wall</keyword>
    <keyword evidence="5" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 5" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="1 5" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041784420" description="Xyloglucan endotransglucosylase/hydrolase" evidence="5">
        <location>
            <begin position="29"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="GH16" evidence="6">
        <location>
            <begin position="30"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7724725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="245" mass="28243" checksum="6D13C002B576F2DF" modified="2024-05-29" version="1" fragment="single">MSRTQIWGGGHMALLCLCMLATAHFVIGAAPKKPVAVPFGRNYVPTWAFDHIKYFNGGSEIQLLLDNYTGTGFQSKGSYLFGHFSMQIKMVPGDSAGTVTAFYSITPKHLDLGIGFLVDDVPIRVFKNSKDLGVRFPFNQPMKIYSSLWNADDWATRGGLEKTDWSKGPFLAHYKNFHVDGCESSVNAKYCETQGKRWWDQKEYQDLDAYQWRRLRWVRKKFTIYNYCTDRKRFPTMAPECKRDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BL39</accession>
    <name>A0AAD5BL39_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">mitogen-activated protein kinase kinase</fullName>
            <ecNumber evidence="7">2.7.12.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_002686</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.12.2" evidence="7"/>
    <dbReference type="EMBL" id="JAMZMK010011982">
        <property type="protein sequence ID" value="KAI7725295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BL39"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004708">
        <property type="term" value="F:MAP kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48013">
        <property type="entry name" value="DUAL SPECIFICITY MITOGEN-ACTIVATED PROTEIN KINASE KINASE 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48013:SF32">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7725295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="118" mass="12886" checksum="FA316407D5761325" modified="2024-05-29" version="1">MPERIIGGSYGYKSDIWSLGLVLLECATGQFPYSPPQPGEGWVSVYELMETVVGQPPPHAPSDKFSPQFCSVQKDPKDRASANELMSHPFISMYDDRAVDLASYFTEAGSPLATLLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BLK6</accession>
    <name>A0AAD5BLK6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_019915</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TALE/BELL homeobox family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011914">
        <property type="protein sequence ID" value="KAI7725551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BLK6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008422">
        <property type="entry name" value="KN_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006563">
        <property type="entry name" value="POX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050224">
        <property type="entry name" value="TALE_homeobox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11850">
        <property type="entry name" value="HOMEOBOX PROTEIN TRANSCRIPTION FACTORS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05920">
        <property type="entry name" value="Homeobox_KN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07526">
        <property type="entry name" value="POX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00574">
        <property type="entry name" value="POX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 8" id="KW-0371">Homeobox</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="10">
        <location>
            <begin position="316"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="8">
        <location>
            <begin position="318"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="175"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="418"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="179"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="190"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="426"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7725551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="450" mass="50432" checksum="78E7475E996A618E" modified="2024-05-29" version="1" fragment="single">MDDDMAMFVRDDSDDDLDGGGGKRKKSFGDGGMVSRGRRGLVGRSGGERSKVKGNGKRSGQSGGHDAEVKEMWDTIAGGDSEDDKEGTRMVHDNNFIHDSGVDPSERSEFPVAHFMTIAVNSGQYMISYAYDGSYGSVGRILDHKYLNPSQELLEEVANLREALSQLKRNRPSNLQKLGVDDKDSKFTTRESTISSSGELSASEKQDLPNKVTKLFSLLDVVDRNYREYYQQLRIVEAALDVVSGSGAARTYTALAHQTISHHFRCLRDAINGQIQLTRKSLGEQDDSADRVLPRLKNVEKQLRQQRNLHHLGGNGHSWRPQRGLPEGSVSILRAWLFEHFLNPYLKDSEKLMLAKQTGLTRSQIANWFINARVRLWKPMIEDIYKEEFGDAKGHCISSQKHAYKSLNDNSSSSEYKELPIIGEPSKSNPNFFNQMEASRVSEPQLSSDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BMW4</accession>
    <name>A0AAD5BMW4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-CH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_015171</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012003">
        <property type="protein sequence ID" value="KAI7725213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BMW4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022143">
        <property type="entry name" value="DUF3675"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033275">
        <property type="entry name" value="MARCH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23012">
        <property type="entry name" value="RING/FYVE/PHD ZINC FINGER DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23012:SF218">
        <property type="entry name" value="ZINC FINGER, RING-CH-TYPE, ZINC FINGER, RING_FYVE_PHD-TYPE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12428">
        <property type="entry name" value="DUF3675"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51292">
        <property type="entry name" value="ZF_RING_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="85"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="124"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="RING-CH-type" evidence="5">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7725213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="205" mass="22609" checksum="FD6E8FF4F978F741" modified="2024-05-29" version="1" fragment="single">YAHRKCVQRWCDEKRDIICEICHQPYKPGYTAPLPQSDDTIINISGNWTISGTPLDLDDPRVLAMAAAEQHFIESDYDEYEENRASGAALCRSVAIILMALLLLKHALTIGDADSDDDDPSVLFAIYLLRAAAFLLPCYIMIWAISMLQRRRQAQEIETLAAADVAFMIQAGQHRGLQVTIAPGTAVAPLPALTPPVIAVHDPLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BNL9</accession>
    <name>A0AAD5BNL9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein uL4c</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">50S ribosomal protein L4, chloroplastic</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_025174</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011625">
        <property type="protein sequence ID" value="KAI7726600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BNL9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1370.10:FF:000012">
        <property type="entry name" value="50S ribosomal protein L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1370.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01328_B">
        <property type="entry name" value="Ribosomal_uL4_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002136">
        <property type="entry name" value="Ribosomal_uL4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013005">
        <property type="entry name" value="Ribosomal_uL4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023574">
        <property type="entry name" value="Ribosomal_uL4_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03953">
        <property type="entry name" value="rplD_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10746">
        <property type="entry name" value="50S RIBOSOMAL PROTEIN L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10746:SF17">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL4C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00573">
        <property type="entry name" value="Ribosomal_L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52166">
        <property type="entry name" value="Ribosomal protein L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0699">rRNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="104"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="259"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="112"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="259"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7726600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="287" mass="31625" checksum="BF9DB87184F1C851" modified="2024-05-29" version="1">MSTFISPPPSSISFLSSSIFSSKTPSSSLFTKPPKPHNLKPTQILSQLSTLPILSFDGTKVGETTLNLKSANPDTARAVVHRGITTDLNNKRRGTASTLTRAEVRGGGKKPFPQKKLGRARRGSQRTPLRPGGGVVFGPKPRDWTVKINKKEKRLAISTALSSAAVNGIVVEEFGDKFEKPKTREFVELLKRWGIDPKEKSMFFMTEVEDNVVLSSRNIGTLKMLTPRTLNLFDILNADKLVFTKGGLEYLNEAYGLVDEEDEDEEEFEEETEGTEAEETVLPPGDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BPF1</accession>
    <name>A0AAD5BPF1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">SCP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_027001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CRISP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011423">
        <property type="protein sequence ID" value="KAI7727188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BPF1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098542">
        <property type="term" value="P:defense response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05381">
        <property type="entry name" value="CAP_PR-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.33.10:FF:000006">
        <property type="entry name" value="Putative pathogenesis-related protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018244">
        <property type="entry name" value="Allrgn_V5/Tpx1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001283">
        <property type="entry name" value="CRISP-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10334">
        <property type="entry name" value="CYSTEINE-RICH SECRETORY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00837">
        <property type="entry name" value="V5TPXLIKE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00198">
        <property type="entry name" value="SCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="PR-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01009">
        <property type="entry name" value="CRISP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01010">
        <property type="entry name" value="CRISP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0568">Pathogenesis-related protein</keyword>
    <keyword evidence="3" id="KW-0611">Plant defense</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042155234" description="SCP domain-containing protein" evidence="6">
        <location>
            <begin position="25"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="SCP" evidence="7">
        <location>
            <begin position="30"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7727188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="165" mass="18712" checksum="B7936118FF3A967D" modified="2024-05-29" version="1">MEVNITKSLVIILLVISCFSLALALPNNQDLGLDFLVPHNKARAQVGVQPLTWNITVAAYARGYAYHRLGDCDMEHSYGPFGENLAEGYGDQFTATDAVNMWVGEKQYYEYESNSCVGDECRHYTQVVWRDSVYLGCAKVKCRNGWWFVICSYDPPGNYEGQRPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BPJ7</accession>
    <name>A0AAD5BPJ7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_021030</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011443">
        <property type="protein sequence ID" value="KAI7727095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BPJ7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7727095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="44" mass="4818" checksum="01E8DA15652D4FB8" modified="2024-05-29" version="1">MVVHGGDCFTINGPSIYIVSYLSLSPATRLIFYVSGPVLRRLGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BSZ9</accession>
    <name>A0AAD5BSZ9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TIR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_019016</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011112">
        <property type="protein sequence ID" value="KAI7728940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BSZ9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10140:FF:000007">
        <property type="entry name" value="Disease resistance protein (TIR-NBS-LRR class)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058192">
        <property type="entry name" value="WHD_ROQ1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11017">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11017:SF307">
        <property type="entry name" value="TIR DOMAIN, P-LOOP CONTAINING NUCLEOSIDE TRIPHOSPHATE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01582">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23282">
        <property type="entry name" value="WHD_ROQ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SMART" id="SM00255">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0611">Plant defense</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="TIR" evidence="6">
        <location>
            <begin position="20"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="771"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="785"/>
            <end position="795"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7728940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="903" mass="101953" checksum="6B9C71AE7037D029" modified="2024-05-29" version="1">MASSSSSGVMASSSAAPEPCIYDVFLSFRGEDTRHSFTDHLYHRLKRAGIHTFRDDEEVNRGEELKPGFERAIKASKASIVVLSENYATSAWCLDELLLILQQRRECNHIVVPVFYHVEPTNVRNQIGSFAIEVKPSSRWTYENVYLWKRALMEVANLSGFTLSGHESSVLKKIVDTMYNKLGPRDVHLPLNITGMATRYEEINSWLYQSNLEFLAIYGMGGSGKTTFAKYIYDSNQKSFEYVSFIENIGSRSKKTNDLLKLQEQLIKDILGGKKRKISGVSQGTCMIEEALQTKRVLIVLDDIVQQSQLDTLLGTGKINSQSKIIITTRERFDMSCWRCQQYEMKLLNADESLELLCRHAFRSKIPMVGFEEAVLQATKYCEGNPLALEVLGSSMSNNTTINYWKSQLNLLDKDIHSGIQSVLIRSYMALPYNTQKELFLHIACFFIGKDVDYVVQILEPDYSAISGIQTLISRCLLSVSPNKKLMMHRLLQEMGKNIVRQESTRFPAERSRVWHSNDSYKILSKENGSEKIEGLALDMKMLSEENFALKVRGWRKTGRPKQVNSSFTELKMIRCIIHGPEYDDIYNIAEMVKSSIGDKTIAFTSSLLEGEMKSGRGTNLTDGKIAETVPLLSPQKIYDKQEIKSDDETDSDSISIFDDMTIQESDLYLREQEEGEGTSKAFAYDDIKSNSPVHDQEIKKIVFTVNLSSRIKRHRMRKALSRVQGIKLIDYDKNERKLTVTGDVDYMTLGARIRKIAHADLLSVGPASERLAKEHGKPGYNSGEEDKPGYESDGRITNSAIGEGENLSSATSAHPCRLFSFAEIKSATKSFNGKLVLRKGGTGTTYKGQISSKEAGHVVAIKRYGTYGRHKKCRNLVIEDVNVERSPYGILQLRFNIFNVEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BVM1</accession>
    <name>A0AAD5BVM1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CRIB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_000927</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010777">
        <property type="protein sequence ID" value="KAI7730457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BVM1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000095">
        <property type="entry name" value="CRIB_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46325:SF48">
        <property type="entry name" value="CRIB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46325">
        <property type="entry name" value="CRIB DOMAIN-CONTAINING PROTEIN RIC8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00786">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50108">
        <property type="entry name" value="CRIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRIB" evidence="2">
        <location>
            <begin position="30"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="143"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="195"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7730457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="206" mass="23036" checksum="5BC09C0FEF155D2A" modified="2024-05-29" version="1">MSTKVKGLIRGIKNISSHIFDDEKKQDIQIGQPTDVKHVAHVGGDGPAVESTSWMWGFDASKERPLDKTGSMVETPAVKWVSEDSTHRKTRKDDSCHRSSRSVETSRELSLIPSSSKNHHRSMDNNIASETLSKDSSSSQSHNTRRHHRGSRRSQDQNAGDGSKIDAPKRSSRRIRKSKDDSLRDGPTQSFTKSTTDDLELRSNDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BWG4</accession>
    <name>A0AAD5BWG4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_013329</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the AP2/ERF transcription factor family. ERF subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010593">
        <property type="protein sequence ID" value="KAI7730907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BWG4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009873">
        <property type="term" value="P:ethylene-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00018">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.730.10:FF:000001">
        <property type="entry name" value="Ethylene-responsive transcription factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.730.10">
        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001471">
        <property type="entry name" value="AP2/ERF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051758">
        <property type="entry name" value="ERF/AP2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31657">
        <property type="entry name" value="ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR ERF061"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31657:SF20">
        <property type="entry name" value="ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR ERF061"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00847">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00367">
        <property type="entry name" value="ETHRSPELEMNT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00380">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51032">
        <property type="entry name" value="AP2_ERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0936">Ethylene signaling pathway</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0611">Plant defense</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="10">
        <location>
            <begin position="31"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7730907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="216" mass="24325" checksum="805F6425CE05105C" modified="2024-05-29" version="1">MGKFSNSQYPNSTENGFFRFSDSYSNPCKKLYRGVRQRHWGKWVAEIRLPQNRVRVWLGTYETAEAAAYAYDRAAYKLRGEYAKLNFANLGDPSQLGLIGDKERLNDLKRAVDAKIQAICQKVRGEKVNRKKNKKKIECESVAMKTESCSSVPCVGDEMILPSSGFENGVWKEESSRCSASDEAKVGAKGESKFEGWPLASLPSYDAELIWEVLAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BZW6</accession>
    <name>A0AAD5BZW6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_024168</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010205">
        <property type="protein sequence ID" value="KAI7732562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BZW6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7732562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="112" mass="12435" checksum="6D831807AC05E1F5" modified="2024-05-29" version="1" fragment="single">VALMKATLHPHLCFLTDRAMGLTGKMNSLSGVTRFPIPHRQGSHPMSQYVRARLPDRNFKHLLSLHIGDQEAEKKLKKVERQHAPAESRQTTITAAGAGEEKQQGQESGMNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C072</accession>
    <name>A0AAD5C072_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_015717</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010416">
        <property type="protein sequence ID" value="KAI7731631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C072"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22162">
        <property type="entry name" value="F-box_AtSKIP3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025886">
        <property type="entry name" value="PP2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32278">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32278:SF11">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14299">
        <property type="entry name" value="PP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7731631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="272" mass="30447" checksum="EE9A69A89CAECDE2" modified="2024-05-29" version="1">MEGLPADCIADILSRGTPRDACRSAVVASLFRTAVESDMLWDSFLPPDHQEIISKSMSPVMYKSKKELFFKLSSPIFIDRGLKAFSIDKATGKNVTPFNKNVTLLGPLILSFGVGNPLTNLELRMTSWLEVEGKINTRILSPNTMYRAYLIVQVAHYRAFGFDVVPCEISVEVCEFCSRGVVTLSHSAFTKRSLEHVCGKNKVGGRLRLRSDDKVSRVCLERKDGWLEIELGEFYNQGMCEKEVKMSLREIKGVHLKGGLIVKSLEIRPVSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C0H7</accession>
    <name>A0AAD5C0H7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nucleoside phosphorylase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_024524</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010185">
        <property type="protein sequence ID" value="KAI7732675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C0H7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009116">
        <property type="term" value="P:nucleoside metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09008">
        <property type="entry name" value="MTAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1580">
        <property type="entry name" value="Nucleoside phosphorylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000845">
        <property type="entry name" value="Nucleoside_phosphorylase_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035994">
        <property type="entry name" value="Nucleoside_phosphorylase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21234:SF42">
        <property type="entry name" value="PHOSPHORYLASE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21234">
        <property type="entry name" value="PURINE NUCLEOSIDE PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01048">
        <property type="entry name" value="PNP_UDP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53167">
        <property type="entry name" value="Purine and uridine phosphorylases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042221935" description="Nucleoside phosphorylase domain-containing protein" evidence="1">
        <location>
            <begin position="26"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoside phosphorylase" evidence="2">
        <location>
            <begin position="45"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7732675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="345" mass="38641" checksum="CFECD0E725E27139" modified="2024-05-29" version="1">MRFFLMIFAWFVVGWLCLFVNRGECMIPKTLLKDIDSVNKVGPYIGVVVPNAFEMNPLLQSSSFIANQTRPYLDIFGRRFRIGILENQRVIIVMTGLSMLNAGVTTQLLLSLFNVKGVLHFGIAGNANTEFEIGDVVIPEFWAHTGLWSWQRYGQGPESELPLESNGDYTRDVGYLKFSDYNNDTTIDNLLNNVWYQPEEVFPINTTPETRQHVFWVPVDKRYFTLAKKLEGMELEECINATTCLPKSPKVARVERGVSANMFIDNGAYRTFLSSKFNVTPIDMESAAVALICYQQKTPFIAIRALSDLAGGGSSISNEASNFAPLAAQNAVATLIKFVQLLQSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C2A2</accession>
    <name>A0AAD5C2A2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_032843</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009852">
        <property type="protein sequence ID" value="KAI7734026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C2A2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032440">
        <property type="term" value="F:2-alkenal reductase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000121">
        <property type="entry name" value="Prostaglandin reductase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045010">
        <property type="entry name" value="MDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43205">
        <property type="entry name" value="PROSTAGLANDIN REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43205:SF7">
        <property type="entry name" value="PROSTAGLANDIN REDUCTASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="2">
        <location>
            <begin position="69"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7734026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="370" mass="41481" checksum="034827590A204EC7" modified="2024-05-29" version="1">MLTENQYFRRSITDGVTVYCGCRRGTNGSLGFRPSAWPPVSLETQRRQQPINGSTVKSVSGVKAHIFHTKILPLVWSDNPPCETLLRKYPNRIFKETIIFLQPIIGYGVARVLDSSHQNFTKGDLIWGLINWEEYSLIKDTSSLVKIQHTNVPLSYYTGILGMPGMTAYVGFYEICSPKKGDTVFVSAASGAVGQLVGQFAKLFGCYVVGSAGSKEKVDLLKNKFGFDEAFNYKEEQDLNVALKRYFPNGIDIYFENVGGKMLEEVLRNMRPHGRIAVCGMISQYNLDQSEGTRNLFLLVARSIRMEGFLVFHHYHKYPKFLEMVLPLIHEGKIAYVEDTVEGLENAPAALIGLYKGTNVGKQVVTIAHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C329</accession>
    <name>A0AAD5C329_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">LIM zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_003398</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009784">
        <property type="protein sequence ID" value="KAI7734197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C329"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140096">
        <property type="term" value="F:catalytic activity, acting on a protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032875">
        <property type="term" value="P:regulation of DNA endoreduplication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd09396">
        <property type="entry name" value="LIM_DA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000078">
        <property type="entry name" value="Protein DA1-related 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045218">
        <property type="entry name" value="DA1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022087">
        <property type="entry name" value="DA1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003903">
        <property type="entry name" value="UIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24209:SF25">
        <property type="entry name" value="PROTEIN DA1-RELATED 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24209">
        <property type="entry name" value="PROTEIN DA1-RELATED 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12315">
        <property type="entry name" value="DA1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00726">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50330">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0440">LIM domain</keyword>
    <keyword evidence="1 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="3 6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="8">
        <location>
            <begin position="123"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="84"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7734197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="366" mass="42068" checksum="358F4BE63DD631AA" modified="2024-05-29" version="1">MGWLSKFFKGSNHEVSEGEYNWRYGDNTVPYGNYPSTSWDPRSEMDDIDRAIAISLAEEDGRGKHVISDDSQLKEDEQLARALQESLRVESPPRHRYGNRNGNGNGNGNMYQPIPFPHSTGFRVCAGCNYEIGHGRFLSCMGALWHPECFRCHACNQPIADYEFSMSGNYPYHKACYKEHYHPKCDVCKHFIPTNAAGLIEYRAHPFWVQKYCPSHEHDGTPRCCSCERMEPRETSYAALNDGRKLCLECLDSSVMDTSECQPLYYDIQAFYESLNMRVEQKIPLLLVERQALNEAMDGERNGHYHMPETRGLCLSEEQTVSTVLRRPRMGMGNRVPDMRTEPYKLTRRCETEVAVMIRILMNGSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C4U5</accession>
    <name>A0AAD5C4U5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tetrahydrofolate synthase</fullName>
            <ecNumber evidence="3">6.3.2.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Folylpoly-gamma-glutamate synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Tetrahydrofolylpolyglutamate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_004944</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>(6S)-5,6,7,8-tetrahydrofolyl-(gamma-L-Glu)(n) + L-glutamate + ATP = (6S)-5,6,7,8-tetrahydrofolyl-(gamma-L-Glu)(n+1) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10580"/>
            <dbReference type="Rhea" id="RHEA-COMP:14738"/>
            <dbReference type="Rhea" id="RHEA-COMP:14740"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:141005"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.17"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; tetrahydrofolylpolyglutamate biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the folylpolyglutamate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.17" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010009569">
        <property type="protein sequence ID" value="KAI7735075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C4U5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004326">
        <property type="term" value="F:tetrahydrofolylpolyglutamate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1190.10:FF:000008">
        <property type="entry name" value="Folylpolyglutamate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="entry name" value="Mur ligase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="entry name" value="Mur-like, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001645">
        <property type="entry name" value="Folylpolyglutamate_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018109">
        <property type="entry name" value="Folylpolyglutamate_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01499">
        <property type="entry name" value="folC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11136:SF16">
        <property type="entry name" value="FOLYLPOLYGLUTAMATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11136">
        <property type="entry name" value="FOLYLPOLYGLUTAMATE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="MurD-like peptide ligases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="MurD-like peptide ligases, peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01012">
        <property type="entry name" value="FOLYLPOLYGLU_SYNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7735075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="461" mass="51122" checksum="00D90A63279E2149" modified="2024-05-29" version="1" fragment="single">ILDLEEPISQMKIIHVAGTKGKGSTCVFTEAILRNYGFHTGLFTSPHLIDIRERFRLDGEDICIEKFLEYFWWCWDRLKACFLIDAKCRDDMPMPTLFHFLSLLAFKIFATEQVDVAIIEVGLGGKRDATNVIQSPIVCGITPLGYDHTEVLGNTLEEIAGQKAGIFKKGVPAFTVSQPPEAMQVLEKTASQLDVCLQVANQLDGNLLNGLHLGLAGEHQYVNAGLAIMLSYMWLQRTGYLEIKPVDLTVSTVKVKHLSCLPEPFIKGLTTASLQGRAQIITDRVCNIQSQGDLVFYLDGAHSPESMEVCANWFSHAIKEDNEQERCSSSTGDNSSKDSIQGPSIAISTTVGRMCLSSSSLPPTSSEVDYSWQMTLQTIWEDIICNKRGNPKKVDPVSSVREYDNDTSVKSCENSMVFSSLPLAINYLRETAEQDKSVRLQVLVTGSIHLIGDVLKLVKKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C5S9</accession>
    <name>A0AAD5C5S9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA topoisomerase (ATP-hydrolyzing)</fullName>
            <ecNumber evidence="3">5.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_018973</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>ATP-dependent breakage, passage and rejoining of double-stranded DNA.</text>
            <dbReference type="EC" id="5.6.2.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the type II topoisomerase GyrA/ParC subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.2" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010009685">
        <property type="protein sequence ID" value="KAI7734629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C5S9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009330">
        <property type="term" value="C:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003918">
        <property type="term" value="F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00187">
        <property type="entry name" value="TOP4c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.268.10:FF:000001">
        <property type="entry name" value="DNA gyrase subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.40:FF:000002">
        <property type="entry name" value="DNA gyrase subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.199.10:FF:000001">
        <property type="entry name" value="DNA gyrase subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.90">
        <property type="entry name" value="DNA gyrase/topoisomerase IV, subunit A, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.199.10">
        <property type="entry name" value="Topoisomerase II, domain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.268.10">
        <property type="entry name" value="Topoisomerase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006691">
        <property type="entry name" value="GyrA/parC_rep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035516">
        <property type="entry name" value="Gyrase/topoIV_suA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013760">
        <property type="entry name" value="Topo_IIA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013758">
        <property type="entry name" value="Topo_IIA_A/C_ab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013757">
        <property type="entry name" value="Topo_IIA_A_a_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002205">
        <property type="entry name" value="Topo_IIA_dom_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050220">
        <property type="entry name" value="Type_II_DNA_Topoisomerases"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004044">
        <property type="entry name" value="PRK05561.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43493:SF5">
        <property type="entry name" value="DNA GYRASE SUBUNIT A, CHLOROPLASTIC_MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43493">
        <property type="entry name" value="DNA GYRASE/TOPOISOMERASE SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03989">
        <property type="entry name" value="DNA_gyraseA_C"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00521">
        <property type="entry name" value="DNA_topoisoIV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00434">
        <property type="entry name" value="TOP4c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101904">
        <property type="entry name" value="GyrA/ParC C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56719">
        <property type="entry name" value="Type II DNA topoisomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52040">
        <property type="entry name" value="TOPO_IIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="Topo IIA-type catalytic" evidence="9">
        <location>
            <begin position="126"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="62"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="active site" description="O-(5'-phospho-DNA)-tyrosine intermediate" evidence="7">
        <location>
            <position position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS52040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7734629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="790" mass="86870" checksum="A2F07A21939A44E3" modified="2024-05-29" version="1">MNHLSSYTPNTTSSSMSFTAGFRFFRCYTQLSLPIPAARFPRQACYTFNNLRFLSSTSRSRRLTSANSKRRESAASEGLDDGGNEGLTAVVNGGDGEGRIVATELHKEATEAYMSYAMSVLLGRALPDVRDGLKPVHRRILYALHELGLSSRKPHKKCARVVGEVLGKFHPHGDNAVYDSLVRMAQDFSLRCPLVSGHGNFGSVDGDNPAAMRYTECRLEALAETMLLSDLDMDTVDFVPNFDNSLKEPSLLPARIPNLLLNGSSGIAVGMATNIPPHNLGELVDALSILIHNPDATLQELLEYMPGPDFPTGGIIMGNLGILDAYRTGKGRVIVRGKTEIETSDSKSKSAAIVVKEIPYQTNKSSLVMKIAELIQNKTLEGISDIRDESDRSGMRIVIELKRGSDPAIVLNNLYRFTALQSSFSCNMVGILDGHPKQMGLKELLQAFLDFRCSVVERRARFKLAHARDRNHIVEGILLGLDNIDAVIDVIRKASSNSAASADLRKKFKLSEKQAEAILDINLRRLTLLEILSLSDGERITSIIPVSEFAEDQYLLMLTANGYIKKVSLMYFSSIRSSGIIALQLVPGDSLKWVRRCTNEDYVAMASQNGFVILSSCEIIRALGRNTRGSVAMRLKEGDRMACVDIIPAAKQDGIEHVPEPDHKHKHPHGPWLLFVSENGYGKRVPLSRFRQSPLNRVGLIGYKFSSEDHLAAVFVVGFTTAEDGESDEQVVLVSQSGTVNRIKVRDIPIQSRYARGVILMRLEHAGKIQSASLISASETEIEEVEAAAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C608</accession>
    <name>A0AAD5C608_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_017882</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009476">
        <property type="protein sequence ID" value="KAI7735524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C608"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43391:SF78">
        <property type="entry name" value="11-BETA-HYDROXYSTEROID DEHYDROGENASE 1B-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43391">
        <property type="entry name" value="RETINOL DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7735524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="117" mass="13011" checksum="63C60BE4805E48ED" modified="2024-05-29" version="1">MKPTSITSTQISSCFTFSCYFCFSFHLFFLFGNSSTPSVHVFGASSGLGEMAYEYAKLGAYLVIIATKKPDSRLELVANNARELGSPDVLFIFADVSKVDECRTFVDETIKRFGRCE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C6I3</accession>
    <name>A0AAD5C6I3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_021504</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009416">
        <property type="protein sequence ID" value="KAI7735915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C6I3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7735915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="96" mass="10974" checksum="F3C7D6443C662BDB" modified="2024-05-29" version="1">MLRQHQIAPFRQRCLHATKHMIQVIYLLFLRKLERVVESDEDCAPGGRRKAMKASFIEATEMKTTPLAAAAAAMLLPSHVLLWWLKNYGDSVKLVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C6V9</accession>
    <name>A0AAD5C6V9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Acid phosphatase/vanadium-dependent haloperoxidase-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_030771</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009463">
        <property type="protein sequence ID" value="KAI7735608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C6V9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003832">
        <property type="entry name" value="DUF212"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31446">
        <property type="entry name" value="ACID PHOSPHATASE/VANADIUM-DEPENDENT HALOPEROXIDASE-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31446:SF2">
        <property type="entry name" value="ACID PHOSPHATASE_VANADIUM-DEPENDENT HALOPEROXIDASE-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02681">
        <property type="entry name" value="DUF212"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="141"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="141"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="155"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7735608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="222" mass="23454" checksum="5328E5F797BA37F1" modified="2024-05-29" version="1" fragment="single">SYAAAVDRQLFTPEYGPEPVTDTNFRQFGTDVILDLAHNKALLAATLCAAAGQLAKPFTASILYKRDFDPKAAISAGGFPSTHSSAAVATAMSVGLERGFSDSVFGLAVVYAALTMYDAQGVRREVGVHARTLNKVLTKNQPNAYASSDPNRSIESSSKKLLSSSEPDNNSLMLEKRVNSLTDIENNCPVSGQLKESIGHTEIEVAAGALLGVLASLAVYSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C822</accession>
    <name>A0AAD5C822_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_020838</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009162">
        <property type="protein sequence ID" value="KAI7736937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C822"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7736937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="39" mass="4290" checksum="FEBC6E47943E9DD9" modified="2024-05-29" version="1">MVAMIQMHRIMELVCHCDGESMVILTSSGEAPLTRLASK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C858</accession>
    <name>A0AAD5C858_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Chloride channel protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">M8C21_015398</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">AA19_3_7</strain>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the chloride channel (TC 2.A.49) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009439">
        <property type="protein sequence ID" value="KAI7735741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C858"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009705">
        <property type="term" value="C:plant-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005247">
        <property type="term" value="F:voltage-gated chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04591">
        <property type="entry name" value="CBS_pair_voltage-gated_CLC_euk_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3080.10:FF:000004">
        <property type="entry name" value="Chloride channel ClC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3080.10">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051280">
        <property type="entry name" value="Cl-channel/antiporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014743">
        <property type="entry name" value="Cl-channel_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002251">
        <property type="entry name" value="Cl_channel_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001807">
        <property type="entry name" value="ClC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11689">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN CLC FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11689:SF92">
        <property type="entry name" value="CHLORIDE CHANNEL-LIKE PROTEIN CLC-G-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00654">
        <property type="entry name" value="Voltage_CLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00762">
        <property type="entry name" value="CLCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01120">
        <property type="entry name" value="CLCHANNELPLT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81340">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13" id="KW-0129">CBS domain</keyword>
    <keyword evidence="12 14" id="KW-0868">Chloride</keyword>
    <keyword evidence="11" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="6" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 14" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 14" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="78"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="121"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="167"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="237"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="267"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="299"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="328"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="361"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="449"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="532"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="15">
        <location>
            <begin position="696"/>
            <end position="757"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU361221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAI7735741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="782" mass="85429" checksum="7B668D3B03862FAF" modified="2024-05-29" version="1">MANGLASNSNGLLPDEESFTSPLLHLRRSTSNNTSQIAIVGSNPCPIESLDYEIIENDVFKQDWRSRSKFQIFEYISLKWILCFVIGIMVSLIGFFNNLAIENIAGVKFVITSNMMFANRYLAAFLIFAAANLGLTLFAVLITTLIAPETAGSGIPEVKAYLNGVDAPAIFSFRTLLIKIVGTIAAVSSSLNVGKAGPMVHTGACIAALTCQGGSKKYGLTWKWFRFFNNDRDRRDLVTCGLAAGIAAAFRAPVGGVLFALEEMASWWRSALLWRAFFSTAVVAILLRGLTDICLSGKCGLFGTGGLIMFDVTSADITYHIVDVPPVLLLGVIGGIAGGLYNFLLGKVLRLYNLINEKGIVYKILLACTVSIITSCLLFGLPWFASCRPCPADASEPCPTIGRSGNYKKFQCQPNEYNELASLFFNTNDDAIKNLFSKDTDTEFHPTSVLIFFITCFFLSILSYGIVAPVGLFIPIIVTGASYGRLVGLLIGSTSNLNHGLFAVLGAASLLGGSMRMTVSLCVILLELTNNLLLLPLIMLVLLVSKTVADAFNGNVYDQIMKLKGFPYLETHAEPYMRQLTVSDVVSGPLRVFNGIELVANIVHVLKTTGHNGFPVVDEPPYSEASILYGLILRSHLITLLKKKAFHVTPTVTDVDSVEQFSSNDFAKQGLGNAEKIEDIELSADEMEMFIDLHPFSNASPYTVVETMSLAKALTLFRGVGLRHLLVVPKTSGRLPVVGILTRHDFMPEHILGLHPLLARSKWKRLRFKFPLWDKLFSSNST</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C8C7</accession>
    <name>A0AAD5C8C7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Vacuolar protein sorting-associated protein 13 VPS13 adaptor binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_001546</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the VPS13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009080">
        <property type="protein sequence ID" value="KAI7737223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C8C7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045053">
        <property type="term" value="P:protein retention in Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026847">
        <property type="entry name" value="VPS13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009543">
        <property type="entry name" value="VPS13_VAB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16166:SF93">
        <property type="entry name" value="INTERMEMBRANE LIPID TRANSFER PROTEIN VPS13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16166">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN VPS13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25036">
        <property type="entry name" value="VPS13_VAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Vacuolar protein sorting-associated protein 13 VPS13 adaptor binding" evidence="2">
        <location>
            <begin position="10"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7737223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="287" mass="32065" checksum="7B4F8E1128FCD5D9" modified="2024-05-29" version="1">MGASAASPNTIRFYVPYWITNDCSIPLTYQVVEIEPLENAALANTKSAKLLSFKSLSSSHPETKSSGNIQVLEAIDDTSPTPSMLSPQDYVGQGGAMLFSSRNDSYLSPRVGLAVAIQHSESFSPGLSLLDLEKKQRVDVKAFSSDGSYYNLSALMQMTSDRTKVIYFQPHTLFYNRAGLSLCLQQCDTQSLLWLHPTDAPSHFRWQTSAKVELLKLKTDGYHWSAPFSVATEGWMRVSLRNETTGKQSYFKVEVRSGTTSSRFDVLFRPNSFSSQYRLSSLLFFRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C8K4</accession>
    <name>A0AAD5C8K4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_020836</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009162">
        <property type="protein sequence ID" value="KAI7736935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C8K4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006502">
        <property type="entry name" value="PDDEXK-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01615">
        <property type="entry name" value="A_thal_3542"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31579:SF2">
        <property type="entry name" value="DUF506 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31579">
        <property type="entry name" value="OS03G0796600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04720">
        <property type="entry name" value="PDDEXK_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7736935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="314" mass="35785" checksum="862CBA58016B5762" modified="2024-05-29" version="1">MGRTTTYIPMSHRVLSPKYDVVDTHRDQPPVQLSGSIFGFLEEEATVLSSLDNDVHYDMQQEIENDDVCEKQEDFDDKIKFWETQRETLHTTLFRTTSLEAKIRNIAKEALGEIEVAGNVCSCSRPVLGGCRSCRMREICSRLQNSGFDSAICKSKWKSSLDLPSGEHTFLDVIDSSNRKKGAVRVIVELELRGQFEMKKGSDEYNSLVCKLPDVFVGKSDRLQTVIKILSVCAKKCMKEKKMHLGPWRKQEYMQAKWLRVIERTTNTTIVSPPLAVEVCYTRPARVKASMLTMDLLDDLPNMSYSYAHVVEVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CB93</accession>
    <name>A0AAD5CB93_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">LOB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_012369</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LOB domain-containing protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008753">
        <property type="protein sequence ID" value="KAI7738677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CB93"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004883">
        <property type="entry name" value="LOB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31301:SF15">
        <property type="entry name" value="LOB DOMAIN-CONTAINING PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31301">
        <property type="entry name" value="LOB DOMAIN-CONTAINING PROTEIN 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03195">
        <property type="entry name" value="LOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50891">
        <property type="entry name" value="LOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LOB" evidence="2">
        <location>
            <begin position="6"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7738677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="173" mass="19080" checksum="E0C5E603FFF0AC93" modified="2024-05-29" version="1">MGSGSSPCASCKLLRRRCAKDCIFAPYFPPDDPHKFAIVHKVFGASNVSKMLQELPIHQRADAVSSLVYEANARMRDPVYGCVGAISYLQNQVSQLQMQLAVAQTEILCIQMHQGTDQLAATHPSMVDCDVKSLLLSGNNTSDFGDHMPQFLSYTSSSNNVIQDPLKRESLWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-10-08" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CBW1</accession>
    <name>A0AAD5CBW1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_005240</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010008679">
        <property type="protein sequence ID" value="KAI7739081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009927">
        <property type="term" value="F:histidine phosphotransfer kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000061">
        <property type="entry name" value="Histidine kinase 5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43047:SF40">
        <property type="entry name" value="HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43047">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="412"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="515"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="662"/>
            <end position="689"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7739081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="715" mass="83708" checksum="FEA70438DE058C6D" modified="2024-05-29" version="1">MEDDHGEVLFSKWLEYVNEAGKKFSVEKPGFHDNMLDDLALIKELIVDFERLVELTSYSEVGSSQLAYLVKNWEYKQENYVRLLREELENLSKQQRHDELKKLEIFDHGFEEENYGGDKPLVSVFDEVHEIFQDGLSKRNDVVEKKPLDVDAEYDTVTYQKQQALHFRKLLEENVHREQILMEKLEESITYLEKQSSPIEELSQILKRADNFLHFVLHNAPIVIGHQDKELRYRFIYNHFPSLVEEFYGQDIIGKTDVEIYKGSGVKEFLDFKREVLERGLPAKREITFVTELFGAITFLMYVEPVFSKDGEKIGVNYMGMEITDQVKKMAKHRQEIALPKDTETELNKTIHITGLTTRNLSNTSDAFSSDDTFTXXXXXXXXXXXXXXLVELTSYSEKGSSQLAYHVKNWEYKQENDVRLLREELENLSKQQRQVELKKLEIYEQHRFEEESYGGDKRPVSVLDEVYEIFQDVPRKRNDVIVENKQLEIDAEYDIVTYWKQQALHLGKLLEASVQREQILLEKLEESIENLEKQSSLVEELSQILKRADNFLHFVLQNAPVVIGHQDKELRYRFIYNHFPSLVKEDVIGKIDVEIFKGGGVKELQDFKRDVLERGLPAKREITFETELFGAKTFLVYVEPVFSKDGEKIGVNYVGMEITDQVKKREKIAKLREEIAVQKAKEMELKKAIHIIESNYNLFPNHVTSEKERKDGKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CC74</accession>
    <name>A0AAD5CC74_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">BED-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_017898</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008747">
        <property type="protein sequence ID" value="KAI7738705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CC74"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003656">
        <property type="entry name" value="Znf_BED"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46951">
        <property type="entry name" value="BED-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46951:SF2">
        <property type="entry name" value="BED-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02892">
        <property type="entry name" value="zf-BED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50808">
        <property type="entry name" value="ZF_BED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BED-type" evidence="6">
        <location>
            <begin position="5"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="BED-type" evidence="6">
        <location>
            <begin position="147"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="214"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="214"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="228"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="244"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7738705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="313" mass="35838" checksum="02C42167AE213AD9" modified="2024-05-29" version="1" fragment="single">MTSIRCGDPGWEHGTAQDDKKKKVRCNYCGKIVSGGIYRLKQHLARVSGEVTYCDKAPEEVCLKMKDNLEGCCRISKKPKQIEYEEQTTYLNFPPCSNDIENDVVEEEHMYDNRNKGKQQQQQLVVVGVDKGLVINMAPPPPLRSLGYVDPGWEHGVPRDERKKKVKCNYCDKIVSGGINRFKQHLARIPGEVAPCKSAPEEVYLKIKENMKWHRTGRRHRRPEPHKQSTTSASTFYTMHSENENENEDDEEEDEQEEDDANLLITSGDKRKRFVRREDAAAFSKKPKSETADAQMLVANNNNNNKISVGRKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CCM1</accession>
    <name>A0AAD5CCM1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_014548</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATPase g subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008871">
        <property type="protein sequence ID" value="KAI7738139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CCM1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015078">
        <property type="term" value="F:proton transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015986">
        <property type="term" value="P:proton motive force-driven ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006808">
        <property type="entry name" value="ATP_synth_F0_gsu_mt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12386">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04718">
        <property type="entry name" value="ATP-synt_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="4" id="KW-0138">CF(0)</keyword>
    <keyword evidence="5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7738139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="122" mass="14076" checksum="70DEE9D135990E69" modified="2024-05-29" version="1">MASKLHMLRSKTAEASNFLSKYGNTYYKKTLEENKKYIVDPPTIEKCQQLSNQLLYTRLASIPSRYDAFRKELDHVKQLIKTRKDIKTEDMGIAALFGLECFAWYCAGEIVGRGFTITGYYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CEB5</accession>
    <name>A0AAD5CEB5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PGG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_026813</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008547">
        <property type="protein sequence ID" value="KAI7739889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CEB5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026961">
        <property type="entry name" value="PGG_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24177">
        <property type="entry name" value="CASKIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24177:SF472">
        <property type="entry name" value="PGG DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13962">
        <property type="entry name" value="PGG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="556"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="123"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="PGG" evidence="4">
        <location>
            <begin position="546"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="14"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="666"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7739889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="666" mass="75111" checksum="77AACB646D942964" modified="2024-05-29" version="1" fragment="single">MAEEIVQVPAIDDNPQTNIEDGSSSNPSPPRQPLWHLIEGSSKVYHEICVPLYHASRTCNWKVANAIFDQNKDMKLLTSSITRHGDTSLHIAAKTEGNKHVDKFVREMVKRMTKVDLELQNSNGNTALYLAAAAGNTRAVQIMVEKNRALLTIAGSNGSMMPLYVAALYGKKDTVKYLYDNSNNLCDDGWTPQNRGWLLLRCVENDIFDTAIEIVKAYPETGTGSVLAALARKPEAFREKKFNIIAKTINWGKRLCSENLSREAEANPNIIGRTITSVSTFMGLKEENALQLLRIIWNNIKEKQTHEIEDILRGPADVKEEDKVASGIVGQTNRLQTLISECILNMNVDIQDTIRSGNEDQSIKLQKVVSDHIVNMNVKTQNLIKEPLGTSNIELSGDRKETFSSRILFIAAEHGNAKFLAELIWGYPHLLRKKNDDGQTIFHIAVKHRHEAIYNMLHEYGLEKQIIAYDKDDNGNNMLHIVGKCPKQKRLEDVSGVALKMQRELLWFKEVEAAIPYYKDKKNKDGLTPYELFTEEHKDMMAQGERWMITTANQCLVVAALIIVMVFAAAITFPGGYKQTSGKDNGSPVFLSKASFWFDRYTPLGTYLIPVNIFFTTKAPSARQLLLQEFFVFSMKFLNIETIKTFSKSSVSLLLGFCDLFYLVSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CEJ2</accession>
    <name>A0AAD5CEJ2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_002366</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008430">
        <property type="protein sequence ID" value="KAI7740431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CEJ2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7740431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="47" mass="5653" checksum="995A4842E07FC5B6" modified="2024-05-29" version="1" fragment="single">MKEDGVCIGRALRDFYWLYSFVIKFPPNNVADVDRRKSTYCIDWIHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CEN9</accession>
    <name>A0AAD5CEN9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Non-specific serine/threonine protein kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_001581</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008598">
        <property type="protein sequence ID" value="KAI7739625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CEN9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048544">
        <property type="term" value="P:recognition of pollen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00028">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01098">
        <property type="entry name" value="PAN_AP_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:001238">
        <property type="entry name" value="Os08g0179000 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.90.10.10">
        <property type="entry name" value="Bulb-type lectin domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001480">
        <property type="entry name" value="Bulb-type_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036426">
        <property type="entry name" value="Bulb-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000858">
        <property type="entry name" value="S_locus_glycoprot_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32444">
        <property type="entry name" value="BULB-TYPE LECTIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32444:SF235">
        <property type="entry name" value="OS01G0783900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01453">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08276">
        <property type="entry name" value="PAN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00954">
        <property type="entry name" value="S_locus_glycop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00108">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00473">
        <property type="entry name" value="PAN_AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51110">
        <property type="entry name" value="alpha-D-mannose-specific plant lectins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50927">
        <property type="entry name" value="BULB_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50948">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="518"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="domain" description="Bulb-type lectin" evidence="6">
        <location>
            <begin position="44"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="7">
        <location>
            <begin position="418"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="586"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="678"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7739625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="678" mass="75518" checksum="C24EED9328196BDD" modified="2024-05-29" version="1" fragment="single">ESDFGADSECGEQEVIPGKAVMPDLNGRGWINGGHRLIKFSNAVDTLSPTEHIRDGDTIVSSGGMFEMGFFNPGNSKNRYLGIWYKKVSYGTVVWVANRDRPLNTTSGILKVSSNAILQLVSVVDTLSPTEHIRDGDTIVSSGGMFEMGFFNPGNSKNRYLGIWYKKVSYGTVVWVANRDRPLNTTSGILQVSNNGILQLVSVVAQLLDNGNLVIREGSRIIWQSFDYPGDTYLPGMKIGSDLSRGIDRQWTTWKSLDDPSPGQYVIWMDTNGFPQIFEKRGSVLHMRFGPWNGVRYSGMPSLRESPIFGNKFVINEKEIYYQLEEVDSSVVMRMYLNPEGVVTRMIWIDRTQSWLSILAVAPTDSGSPYGLCGPYGTCNSKNFPICSCLDGFVPKRAKDWKAGDWTSGCELRTPLECGDGNAIGFKRVSGVKLPDTRSSRYNLSMNLWECEMECRRHCECTAYANLYVIGSGCLLWFDNLMDIREADETQDLYIRMALSELPSFTTPESASNKKKNMIIAVVSTLICLVLIGLTLAVYASMKKSRSYLKGRVHDFNEDTIGDGPEEDVDLPSFSLSTIFKSTNNFSTSNKLGEGGFGPVYKGGFSQNPGKELIGGVLDDGREIAVKQLSKSSRQGLDEFKNEVRCIAKLQHRNLVKLLGYCIEGDETMLIYEFMANR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CGP1</accession>
    <name>A0AAD5CGP1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Translocon-associated protein subunit alpha</fullName>
            <shortName evidence="7">TRAP-alpha</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Signal sequence receptor subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_027690</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">TRAP proteins are part of a complex whose function is to bind calcium to the ER membrane and thereby regulate the retention of ER resident proteins. May be involved in the recycling of the translocation apparatus after completion of the translocation process or may function as a membrane-bound chaperone facilitating folding of translocated proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Heterotetramer of TRAP-alpha, TRAP-beta, TRAP-delta and TRAP-gamma.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Endoplasmic reticulum membrane</location>
            <topology evidence="1 7">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">Shows a remarkable charge distribution with the N-terminus being highly negatively charged, and the cytoplasmic C-terminus positively charged.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the TRAP-alpha family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008402">
        <property type="protein sequence ID" value="KAI7740570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CGP1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005595">
        <property type="entry name" value="TRAP_alpha"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12924:SF7">
        <property type="entry name" value="TRANSLOCON-ASSOCIATED PROTEIN SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12924">
        <property type="entry name" value="TRANSLOCON-ASSOCIATED PROTEIN, ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03896">
        <property type="entry name" value="TRAP_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="4 7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7 8" id="KW-0732">Signal</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042148314" description="Translocon-associated protein subunit alpha" evidence="8">
        <location>
            <begin position="27"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="195"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU368074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7740570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="262" mass="28281" checksum="DB84C29ADB13555F" modified="2024-05-29" version="1">MAKIRVSFIIALLLLSSSLFLQVARCQSESDSEDAELVAETVEEGGDLGIVGDDVQDFGGGNYSPAPGVETVCVFPKNAAKTVVAGKETEILVGMQNEGDQSVKVLAVHASVHLPFDHRMLVQNLSTQVFNNASIPPSVQAAFPYIFAVSKFLQAGTFDLVGTIVYEIDQLPYQNTFYNGTIEVTEAGGLVSVETVFLVSLGISLLVFLGLWVRGQIQNLSKKTKKAPKVETGTRNVDASMDEWLEGTAYTQSKANKSKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CGU0</accession>
    <name>A0AAD5CGU0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NAB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_003655</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008154">
        <property type="protein sequence ID" value="KAI7741647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CGU0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011684">
        <property type="entry name" value="NAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056889">
        <property type="entry name" value="NET2A-D/KIP1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056888">
        <property type="entry name" value="NET2A-D/KIP1-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31631">
        <property type="entry name" value="PROTEIN NETWORKED 2D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31631:SF0">
        <property type="entry name" value="PROTEIN NETWORKED 2D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07765">
        <property type="entry name" value="KIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25014">
        <property type="entry name" value="NET2A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24918">
        <property type="entry name" value="NET2A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51774">
        <property type="entry name" value="NAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAB" evidence="4">
        <location>
            <begin position="10"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="108"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="172"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="256"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="517"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="120"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="135"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="148"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="162"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7741647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="613" mass="70920" checksum="F685E3DB83A36F02" modified="2024-05-29" version="1">MTERATGKGYSWWWASHIRTKQSKWLEQNLQDMEDKVEHVLNLITKDGDSFARRAEMYYKHRPEVINFVEETVRAYRSLAERYDKLSTDLQKANTTIATVCPDKVTYEDDDDYTVGPSKLPKKPSKKASKKASKIPKAPKLPAKGVLNNYSKKTTKTNPNHVESDSTKDDELKSCEETLKMLQEKQEQLIEEAKVEQQRFEDAKSKLESLKHKFNLYDDEDENDVDSETEVEETIDKLMNKMTTLETSVTSQTTLTDTLRKENHDLQTRIQNLEAEKAALMSTKNVVNVESKAGKTDQAYWVEMLLNGYEYRDDILLEQYVAILKSYKDTKKKLSDEQTKNQQTLSSMNSAIVMRDNKIQEMIQKLKHLQESLGEDKVEIPSVEFLDEPQVVSASEERLRKNIDAILDQNLDLWLRLSTTFRQVHKFKKQVKDTLEEVKQVEAKGLTGKSDTSMFTSDLLSEIKPMYKHLHKINTRLASWLQQSILLNNELDTINSYLCNIQEEITQEEIKFSAHQAAKFKGDILNMKQENNKVNEELEAGLDHAIALKHEIEQTLERLERKFGLSANQNQPRKRWSSRRSLSLRSLLFGGTSKKQNSCLFSCLGHHKRTHSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CH84</accession>
    <name>A0AAD5CH84_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_028186</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008193">
        <property type="protein sequence ID" value="KAI7741464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CH84"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025066">
        <property type="entry name" value="CCDC174-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15885">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 174"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15885:SF1">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 174"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="202"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7741464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="256" mass="29291" checksum="1F04A038F129AEE2" modified="2024-05-29" version="1">MADDETTKKKRVVVESLGWLTESSIMPKKHRAIAGVGPSSILELKAQLYKSQEEAKTTNLNNNNNNNPDQIEFHRAKKKIVPNDVFTAKNSGVDARAHKDKLEMKAERDGSASYAALEKKAELYEKLARGEVSDEEDKEKYCVDFFNKTYVNDVPQRHSPFSIETNKHESDTHDEPMLPDTRTMGLGRAAGTLDNDEHKRFVREVHEEVNQAREKVSELKVRRQEQVAARREKLKQAYLRKQLEKLKAQSKQGNDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-06-18" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CKB3</accession>
    <name>A0AAD5CKB3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ATP-dependent Clp protease proteolytic subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_014035</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Component of the chloroplastic Clp protease core complex which consist of at least 16 proteins: CLPP4 (3 copies), CLPP5 (3 copies), CLPR4 (2 copies), ClpP1 (1 copy), CLPP6 (1 copy), CLPR2 (1 copy), CLPT1 (1 copy), CLPT2 (1 copy) and 3 copies of CLPP3 and/or CLPR1 and/or CLPR3. The core complex is organized in two heptameric rings, one containing CLPP3,4,5,6 in a 1:2:3:1 ratio and the other CLPP1 and CLPR1,2,3,4 in a 3:1:1:1:1 ratio.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the peptidase S14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007734">
        <property type="protein sequence ID" value="KAI7743408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009368">
        <property type="term" value="C:endopeptidase Clp complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009532">
        <property type="term" value="C:plastid stroma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051117">
        <property type="term" value="F:ATPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07017">
        <property type="entry name" value="S14_ClpP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000020">
        <property type="entry name" value="ATP-dependent Clp protease proteolytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001907">
        <property type="entry name" value="ClpP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023562">
        <property type="entry name" value="ClpP/TepA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10381">
        <property type="entry name" value="ATP-DEPENDENT CLP PROTEASE PROTEOLYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10381:SF47">
        <property type="entry name" value="ATP-DEPENDENT CLP PROTEASE PROTEOLYTIC SUBUNIT-RELATED PROTEIN 4, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00574">
        <property type="entry name" value="CLP_protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00127">
        <property type="entry name" value="CLPPROTEASEP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="13"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00062827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7743408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="290" mass="32341" checksum="32B03A30B98D164C" modified="2024-05-29" version="1">MATMGSHIFPTTHLPSKPNSTHNRPVPIKASLSTNFISPYVGTSLSSQFSGYKIRPASLNPSLTSHGKRLGVTMVIPFSRGSAWEQPPPDLASYLYKNRIVYLGMSLVPSVTELILAEFLYLQYEDDKKPIYLYVNSTGTTKGGEKLGYETEAFAIYDVMRYVKPPIFTLCVGNAWGEAALLLAAGAKGNRSALPSSTIMIKQPIARFQGQATDVEIMRREIKNVKTELVKLYSKHIGKTTEQIEEDIRRPKYXXXXEAVEYGIIDKVIYNERGNQDRGVLSDLKRAQLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CKD6</accession>
    <name>A0AAD5CKD6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_003129</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007778">
        <property type="protein sequence ID" value="KAI7743174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CKD6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7743174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="57" mass="6783" checksum="6749C520FE38947A" modified="2024-05-29" version="1">MMNLHFYMFSKKVRVFLIEVHFWGLLRAGHQEFFYHMLPCRLQAGLKFVIYGMDGAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CLL8</accession>
    <name>A0AAD5CLL8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DCD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_007109</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007829">
        <property type="protein sequence ID" value="KAI7743060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CLL8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034976">
        <property type="term" value="P:response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013989">
        <property type="entry name" value="Dev_and_cell_death_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011043">
        <property type="entry name" value="Gal_Oxase/kelch_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044832">
        <property type="entry name" value="NRP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46034">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46034:SF7">
        <property type="entry name" value="INFLUENZA VIRUS NS1A-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10539">
        <property type="entry name" value="Dev_Cell_Death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00767">
        <property type="entry name" value="DCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50965">
        <property type="entry name" value="Galactose oxidase, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51222">
        <property type="entry name" value="DCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DCD" evidence="2">
        <location>
            <begin position="21"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="245"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="246"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7743060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="648" mass="71804" checksum="A8800F36A7BE40E5" modified="2024-05-29" version="1">MGARKKESNYGPSNNRNLGKSQLGGVIFGATNLTINECLSKQLFGLPKEHLEYVEKISPGLPLFLFNYTDKKLHGIFEAASHGQKDIDPYAWTIEGKQRTPFPAQVQVRLQSARQPLTETQFKPAIADNYYNRVHFWFELDHSQTNRLLSLFSSQPVAASLFTTNFPFLPKKVETGLKEKKLCMSFSGNTVNSNTLDERLGTVVKDEKEQVCMKLKELALNRKIINPPPTAREDEAARVKLYPHSATREDETARKNVEPPSAAHEDDMTVGTSTSSMEDPIIAQLSEKVDYLMAFITEQNYKIEYLEKRVIFLEQNLAPQNEIQCLKDPSMPGPSEESHAINGITSANESDLGNSPDINLDSCDDLIFLVGGYDGMSWFSSLDAYSPSNNCIKSLKPMNTARCYAPVSVLNGELYVFGGGTRGVWYDTVESYDPIVNEWTSRPALNAKKGSLAGATLNGKIYAVGGGSDDIFYSAVEMLDLDIGAWIPSRSMLHKRFSLAAAELNGAIYAVGGYDGTSYLKSAERFDPREHSWTTIESMNTMRGCPCLVVLNEKLYVVGGFDGNEMVPTVEIYDPRRGSWMIGEPMKYPRGFSAAAVVKESIYVFGGLKSGDEINHTVECYKEGQGWENVDAKAAGRRCFFSAVGLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CLV7</accession>
    <name>A0AAD5CLV7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_008731</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007856">
        <property type="protein sequence ID" value="KAI7742926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CLV7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7742926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="173" mass="20010" checksum="9634DBFF66814F9E" modified="2024-05-29" version="1" fragment="single">KAKATDGNESERMLHSGTNDMNGVNTDHASRAKPQFSGVENYHFRMLIAPRGLEEYHDNHGDLDDLDEKEIAQENIQRFITTLSNLKCTITWAVWGELFYRLYILKSSTKHQNSIRFNNQATQHGACPRDDKGYQHPLKALHARLGRSKFYNIQPPLLIIIYTQAHKQLNKYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CM30</accession>
    <name>A0AAD5CM30_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">M8C21_013598</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">AA19_3_7</strain>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010007813">
        <property type="protein sequence ID" value="KAI7743104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CM30"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009742">
        <property type="term" value="P:brassinosteroid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000016">
        <property type="entry name" value="probable serine/threonine-protein kinase At4g35230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000154">
        <property type="entry name" value="probable serine/threonine-protein kinase At4g35230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000069">
        <property type="entry name" value="probable serine/threonine-protein kinase At5g41260"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045845">
        <property type="entry name" value="BSK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058209">
        <property type="entry name" value="TPR_BSK1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45863">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE BSK5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45863:SF51">
        <property type="entry name" value="TRANSFERASE, PROTEIN KINASE RLK-PELLE-RLCK-XII-1 FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25575">
        <property type="entry name" value="TPR_BSK1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1070">Brassinosteroid signaling pathway</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0519">Myristate</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="57"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAI7743104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="461" mass="51493" checksum="631E02644C0B9AEC" modified="2024-05-29" version="1">MGCEFSKLCSCCDTVQDGMVAAPANAENDLGEASDLPAFREYSIEQLRTATSGFSIENIVSEHGEKAPNVVYKGKLANQRRIAVKRFNRSAWPDSRQFLEEARAVGQLRNPRLANLLGCCCEGDERLLVAEFMPHETLAKHLFHWETQPMQWAMRLRVALYLAEALEYCTNKGRALYHDLNAYRVVFDDDSNPRLSCFGLMKNSRDGKSYSTNLAFTPPEYLRTGRVTPESVTYSFGTLLLDLLSGKHIPPSHALDLIRDRNLQMLTDSCLEGQFSNDDGTELVRLASRCLQYEPRERPNPKSLVSALLPLQKETEVPSHVLMGIAQGGSAMPLTALGEACLRTDLTFIHGVLEKLGYKDDEGDSAFRHKEFKAAIDYYTQFIDVGTMVSPTVFARRSLSHLMNDNPQEALNDAVQAQVISPIWHIASYLQAAALFALGRENEAQIALREGTVLEEKHEAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CNH2</accession>
    <name>A0AAD5CNH2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_026960</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010007666">
        <property type="protein sequence ID" value="KAI7743824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CNH2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000085">
        <property type="entry name" value="CDPK-related kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000101">
        <property type="entry name" value="CDPK-related kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001294">
        <property type="entry name" value="CDPK-related kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="85"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7743824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="458" mass="52236" checksum="5459E21D45AEA23A" modified="2024-05-29" version="1" fragment="single">ISCNKRNNHNAGEEKSTFLTFYPGKYLFSKKLMNATPKRFFRRAFAPLSPAQHMKGVLARRTGKGGGEDKLNKSFGFLKHIGHKYDIGEEVGKGHFGHTCKARCKKGEFKGQQVAVKIISKSQMTTAIAIEDVHREVKILRALTGHNNLIKFYDAYEDHDFVFLVMELCEGGVLLDRILSRGGKYSEYDAKGVLIQMLSVVAFCHLQGVVHRDLKPEVRLLKDEPRFNEALWSDLSFEAKDFVKTLLNKDPRKRLTAAQALCHPWIRNDSQVKTPFDISILKFVKCFICASNLRKSALRALSKTLIFDELVYLKEQFSLLEPSINGFISVENLKIALMKYTTEAMKESGVYDFLESLCKIQYRRMDFEEFCAAALRVHQLEGHERWEKQTRDAYEIFEKDGNRAIVVEELASELGLGPAIPVRDVMDDWVRPSDGNLTYIGFVKVLHGTKSRNLAKPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CP13</accession>
    <name>A0AAD5CP13_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_022043</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase A1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007355">
        <property type="protein sequence ID" value="KAI7745132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CP13"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032861">
        <property type="entry name" value="TAXi_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13683">
        <property type="entry name" value="ASPARTYL PROTEASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13683:SF800">
        <property type="entry name" value="EUKARYOTIC ASPARTYL PROTEASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14543">
        <property type="entry name" value="TAXi_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase A1" evidence="2">
        <location>
            <begin position="1"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7745132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="173" mass="18659" checksum="5AFAAAED9DCEFB3D" modified="2024-05-29" version="1" fragment="single">MAPHPYYRPNNDLVPCKHPLCASLHSGDHICDDPDQCDYEVEYADGGSSLGVLINDVSLVNLTTGVRITPRLTIGCGYDQISGPSYQPLDGVLGLGKGKTGIVSQLHSQGLIRNVVGHCFSSRGGGFLFFGDDVYDPSRVISTPMSRDYRKHYSPGFGEVIYGGKPTGLKNLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CPC5</accession>
    <name>A0AAD5CPC5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_011023</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007159">
        <property type="protein sequence ID" value="KAI7745681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CPC5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43977">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7745681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="62" mass="7031" checksum="E63D9B6FA2CFB256" modified="2024-05-29" version="1">MVRRLADVTSTQFITTTFHPELVKVADKVYGVTQKNEVSRVNVVTMDEALDFIVHDQSHKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CQ34</accession>
    <name>A0AAD5CQ34_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CNH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_017243</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007051">
        <property type="protein sequence ID" value="KAI7746033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CQ34"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034058">
        <property type="term" value="P:endosomal vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001180">
        <property type="entry name" value="CNH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032914">
        <property type="entry name" value="Vam6/VPS39/TRAP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019452">
        <property type="entry name" value="VPS39/TGF_beta_rcpt-assoc_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019453">
        <property type="entry name" value="VPS39/TGFA1_Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12894">
        <property type="entry name" value="CNH DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12894:SF43">
        <property type="entry name" value="VACUOLAR SORTING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10366">
        <property type="entry name" value="Vps39_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10367">
        <property type="entry name" value="zf-Vps39_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50219">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CNH" evidence="1">
        <location>
            <begin position="25"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="940"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7746033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="940" mass="104510" checksum="D60FE98C3CF5D92B" modified="2024-05-29" version="1" fragment="single">IIICTSLKIFNILYVTQPRRIGVNDRSINPIFLYINGGKFEALSPKTHLWPNPISNHAQSSNPFQNSTHLQPQSLVKPVKKIGALKGVSVVARRLRSDVNECFSKLTVGGSDAVGGSSFLQRLGGGVGSRVNGGGGGGGVNDLPIDDNCVFAVAVEKKLILVGLVGRSSENYDSVAGSLVTLREISCVDVVKDLVWVNDSMIVGCSSGYYLCSCVTGQCGLIFSFPDVSSVLRLKVLKKDYKILMLVDNVGIIVDSHGQPVGGSLVFNGSPDGIGEMGSCVAAIHSGKMELYAKKSGNCVQKVVFGGEGRGNHVIADDEDGNGKAVVVATPNKVICYLKVPSEEQIKDLLKKKDFKEAVSLSVELHDDGEISKETLSLVHAQVGFLLMFDLHFEEAVNHFLLSETMQPSEIFPFIMPDPNRWSLLVPKNRYWGLHPPPAPLESLETAVDDEFLLNPPNRDDLLDTAIKNLIRYLNVSREKDLTASVREGVDTLLMYLYRALSCVDEMESLASSENWCIVEELETLLNDSGHLRTLAFLCESKGMSSKALAIWRILARNYSSGFWADPAKTNEKHDLGANIISGKENAATEATRILVELSDQDLILQHLGWYWSNPWTNINNMLAVRVLTSEKRSHQLSPDEVIAAIDPKKTEIIQRYLQWLIEDQGSDEPQFHTSYALLLTKSALETYGTESQSEQINGSESGNHSIFQSPVRERLQVFLQSSDLYDREEILYLIEESELWLEKAILYRKLGQETLVLQILAVKLEDSDAAEHYCAEIGRPDAYMQLLDIYLNPTDGKKPMFKAAVRLLHNHGESLDPLQVLERLSPNMPLHLASDTILRMLRARRHHHYQGQVVHNLSRAVNLDAKLARLEERSRLVQINDESLCDSCHARLGTKLFAMYPDDTIVCYKCFRRQGESTSVTGRDFNNDPVFKPGWLVME</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CTE8</accession>
    <name>A0AAD5CTE8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phosphatidate cytidylyltransferase, mitochondrial</fullName>
            <ecNumber evidence="6">2.7.7.41</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">CDP-diacylglycerol synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">M8C21_021170</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">AA19_3_7</strain>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 3/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TAM41 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.41" evidence="6"/>
    <dbReference type="EMBL" id="JAMZMK010006870">
        <property type="protein sequence ID" value="KAI7746955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CTE8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004605">
        <property type="term" value="F:phosphatidate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032049">
        <property type="term" value="P:cardiolipin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016024">
        <property type="term" value="P:CDP-diacylglycerol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015222">
        <property type="entry name" value="Tam41"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13619">
        <property type="entry name" value="PHOSPHATIDATE CYTIDYLYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13619:SF0">
        <property type="entry name" value="PHOSPHATIDATE CYTIDYLYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09139">
        <property type="entry name" value="Tam41_Mmp37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028840">
        <property type="entry name" value="Mmp37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="10" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="16" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="17" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00018337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00029893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAI7746955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="328" mass="36512" checksum="8A64BE17B3D5089A" modified="2024-05-29" version="1">METTTSLGSLLKVLPPVDFCCVYGSSLHPNNNDKSSMEDYILGVSDPQQWHSENLKLNRGHYASWMASVGGAKLITKAADRIGVGVHFNPFVSWNNKMYKYGVVRMDDLVEDILSWKRFYLSGRLQKPVRIIVDNRKIANTNIINLRAASSAALLLLPSKFTERELYAKICSLSYMGDLRMFFAEDKNKVKKIVEGQFELFRNMYKPLVEEYATKQLLRLSSCGDHQMSISQDCSLSATSSVVSSLPESVRSQMGVGTVLTKAGQNSKLTIGSREEAATLMQKVLRRRVMISSARQAVAGFIAVGAVQGVKYLGEKMTKAWNSSSKII</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CUP9</accession>
    <name>A0AAD5CUP9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_012428</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006496">
        <property type="protein sequence ID" value="KAI7748628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CUP9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030015">
        <property type="term" value="C:CCR4-NOT core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000288">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040398">
        <property type="entry name" value="Not1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13162">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13162:SF8">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7748628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="404" mass="45438" checksum="D507CFC4DFFF8E29" modified="2024-05-29" version="1">MIPLAQSLSTEIRLLFESLNESNHDSVVRELFQYVDYGVEGSILLLETCLDHFKVYEKDLTSTHLEPLVASLFRKLLEKPQFSTVFSMSVRSSAITEEFLVKVSAALQLTTNEKLGFGLALTDSESNDIRMSGRNFCMRQIEELCATHASLHTADYVQDVLLFLNKSEALSKHVDSFMQLLSLVQFDKDSDFILAPLLSDEFHNSKLLRHMDFLNEQNENEFDDILAQMEREMNMADMLVELGYKCTSDVSLCKDVLSSFSPLTEVTVARILGTIIRSDAGLQDNENALSTFFSSIGRGTLSDMSALNSWNTDVLIESIKQLAPGISWTTVIENLDHEGFYVPDEAAFSLLISCYRHASQDPFPLAAVCGNVWRNTEGQLSFLKYAISVPPEVFTFAHCKRQLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CVA1</accession>
    <name>A0AAD5CVA1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cytochrome P450 89A2-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_033883</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006419">
        <property type="protein sequence ID" value="KAI7748966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CVA1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016709">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11075">
        <property type="entry name" value="CYP77_89"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051103">
        <property type="entry name" value="Plant_metabolite_P450s"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24298:SF800">
        <property type="entry name" value="CYTOCHROME P450 89A2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24298">
        <property type="entry name" value="FLAVONOID 3'-MONOOXYGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0349">Heme</keyword>
    <keyword evidence="7 8" id="KW-0408">Iron</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="7">
        <location>
            <position position="454"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7748966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="515" mass="59578" checksum="1C9F6C93958AFA5F" modified="2024-05-29" version="1">METTFLTVIISLITTIFLVHTLFRRNTIKKLPPGPSFITSNYLLLTNPLLQFEPVLKNLKTIYGPFFTLSTCFGPAIFISDHSLAHQVLIQYGAVFSDRPKSFPMRNISGSSYGPTWRLFRKNLASEFMHPSRIKSYSWARKWVLQVFFGRVRDHKNEVNGMIKVIDHYRYAMFCLLVFMCFGERFEESRINEIAKVQKDMLLKVSSGRFGVFNVFPRLGKILFRERWREFEKLCSDKEDVLIPLIKARIELDGYEGRNVAYVDTLVNLRLPEEEGDNGNGGKLSYKEMVSMCSEFFNSGTDTTSTALQWIMANLVKYPHIQRRLYDEIVRVVGAPPPPPQKGVEVELESVINEEDIEKMPYLKAVVLEGLRRHPPVHFVLPHRVMKEVEVQGYRIPQGASINFMVGEMGLDPKVWDEPMEFKPERFLVNDAIFDVSGRKEIKMMPFGAGRRMCPGYELALFHLQYFVANSIWYFDWSVPDGYDVDLSEEVELTVVMKNPLRARVSSRVEKRTIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CVC6</accession>
    <name>A0AAD5CVC6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Inositol polyphosphate-related phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_033900</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the inositol polyphosphate 5-phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006419">
        <property type="protein sequence ID" value="KAI7748983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CVC6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004445">
        <property type="term" value="F:inositol-polyphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034485">
        <property type="term" value="F:phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004439">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045849">
        <property type="entry name" value="IP5P_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000300">
        <property type="entry name" value="IPPc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45666">
        <property type="entry name" value="TYPE IV INOSITOL POLYPHOSPHATE 5-PHOSPHATASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45666:SF18">
        <property type="entry name" value="TYPE IV INOSITOL POLYPHOSPHATE 5-PHOSPHATASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22669">
        <property type="entry name" value="Exo_endo_phos2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00128">
        <property type="entry name" value="IPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Inositol polyphosphate-related phosphatase" evidence="3">
        <location>
            <begin position="57"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7748983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="328" mass="37135" checksum="6E348208DDC4AF40" modified="2024-05-29" version="1" fragment="single">VMWPRLVASKLLRKTLGSNNFVADIPGNTNSFLDLPTLDLESVPSPKIVLTDHHDTRKFKIFVSTWNIGGVTPTEDLNIEELFQEVVPLKASNVLGPDKKKISAKWNSLIRKALNKKSYSGYYKSNSLSKKQQLVTKGNKLDIESSMIQPEFRCLISKQMVGIMISVWVRSDLHPFFQNPSVSCVGCGIMGCLGNKGSVSVRFQFHETSFCFVCCHLASGAREGDERIRNSNAAEILSRTCFPTNIGPSLDLPKKILDHDRVILLGDLNYRISLPEKETRLLVNRNDWNTLLENDQDNLDWRGIETTAIHKKRNPIVRSQTREIYVLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CVT0</accession>
    <name>A0AAD5CVT0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">histone acetyltransferase</fullName>
            <ecNumber evidence="2">2.3.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_032810</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-lysyl-[protein] + acetyl-CoA = N(6)-acetyl-L-lysyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45948"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.48"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the HAT1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010006451">
        <property type="protein sequence ID" value="KAI7748889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CVT0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004402">
        <property type="term" value="F:histone acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031509">
        <property type="term" value="P:subtelomeric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.360.10:FF:000002">
        <property type="entry name" value="Histone acetyltransferase type B catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.360.10">
        <property type="entry name" value="Histone acetyl transferase 1 (HAT1), N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019467">
        <property type="entry name" value="Hat1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037113">
        <property type="entry name" value="Hat1_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017380">
        <property type="entry name" value="Hist_AcTrfase_B-typ_cat-su"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12046">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE TYPE B CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10394">
        <property type="entry name" value="Hat1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Histone acetyl transferase HAT1 N-terminal" evidence="8">
        <location>
            <begin position="31"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetyltransferase" evidence="7">
        <location>
            <begin position="214"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF10394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7748889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="457" mass="51735" checksum="511A54AB6E2E5DB4" modified="2024-05-29" version="1">MGTKHQSSNSLSDPKKRRKVGFTKVDEGLQPNDCIKIYIVSKEDEVGSEESFSIEPVDLSSFFEEDGKIYGYQGLKITVWISSISFQAYADISFESTCDAGKGITDLKASLQNIFADNLVETKNDFLKTFSTETHYIKSMISDGKIISNGLDGHLKNDCSELEVVRVEGQTMGLLYCRLVPLVLLLVDGSNPIDVTDPDWQIYLLVEKKNDEQDTPQKLLGFAALYRFFHYPDSHRLRLGQILVFPPYQRKGYGGYLLQAITSMVVADNMYDLSIEEPLDSLQHVRACIDIPRLLALPAIQPSLNATVSRLKEENLAKRTQITRFSPPSDTIEEVRKTLKINKKQFLQCWEILIYLALDPIEKYLENFRTVVLDRIRGDVIGKDTGASGKRLVEVPTEFDSEVSFVMFKTNGSGEEFKEAEVEESDKVKQEEQLQKLVDERIEAIKLVAEKVRVKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CX21</accession>
    <name>A0AAD5CX21_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Peptidase S59 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_001857</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleoporin GLFG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006348">
        <property type="protein sequence ID" value="KAI7749492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CX21"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044614">
        <property type="term" value="C:nuclear pore cytoplasmic filaments"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008139">
        <property type="term" value="F:nuclear localization sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000973">
        <property type="term" value="P:post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006405">
        <property type="term" value="P:RNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034398">
        <property type="term" value="P:telomere tethering at nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.2360:FF:000001">
        <property type="entry name" value="Nuclear pore complex protein Nup98-Nup96"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037665">
        <property type="entry name" value="Nucleoporin_S59-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007230">
        <property type="entry name" value="Nup98_auto-Pept-S59_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024945">
        <property type="entry name" value="Spt5_C_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23198">
        <property type="entry name" value="NUCLEOPORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23198:SF32">
        <property type="entry name" value="NUCLEOPORIN AUTOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01104">
        <property type="entry name" value="CTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51434">
        <property type="entry name" value="NUP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="7" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0811">Translocation</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Peptidase S59" evidence="10">
        <location>
            <begin position="866"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="19"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="118"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="334"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="524"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="761"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="19"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="118"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="146"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="359"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="384"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="414"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="431"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="524"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="787"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="811"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="886"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7749492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="886" mass="89245" checksum="AD6E6A40F4A9F744" modified="2024-05-29" version="1" fragment="single">MLGGAGYSFGQSSNSPFGSPSVFGQTSNANSNPFAAQPFGSTSPFGSQTGGSIFGGTSTGVFGTQAPSPVFGASSAPAFGSSTPAFGASSSPAFGASSSSFGGSSVFGQTPAFGGFGSTTQSSPFGSSFQQSQPAFGSSVFGSNQPAFGAPSPTTPTFGTSSTPAFGATSTPAFGATSTPAFGSTSTPTFGNTGSAFGVSSTSVFGSTTPAFGASSTPAFGASSTPAFGASSTPAFGASSTPAFGASSTPAFGSSPTPAFGATTSPFSFGSSPAFGQSTSAFGSSPFGATTTAFGTQSSMFGGQTTTPAFGSPGFGQASFGTQRGGSRVTAYAHTPEADSGSSTQPTGKLESISAMPAYKEKSHEEIRWEDYQLGDKGGPNPAAAQSSAGMGFNMNNTQQTNPFSSPSPAFGQSSANPFSSSTSTNPFAPKTSTFGTPGFGSSTPSLGSSPFAAAASNPFGSASTPAPSAFGSTPAFGSTTSPSLFGTPSSSTFGTSTSIFGTTQAQGTSSFATGLNFGNTQSSSLFQSSTPTLGQTSSPFGQAPSAFGQAPSAFGQAPSAFGQAAPSFGQSTPAFGQSNAFGGNLFSSTPSLLSTNSMGFNQTTPSLSTPFQSAQPTQNPGGFGFATPAGSTPSIFGQSNFGQLSANQSPHPQSITNPFGTLPAMPQMSIGGVGNAPSVQYGISSLPVVDKPSPIRLSSVLTSRHLSQRRIRLPARKYHPNGSKVSFFSDEEDTTSTPKADALFIPRENPRALVIRPMEQWPGKASAEKPKEKNVSSPAQADGQYTEFTYTAHQNGRSTDNGNGKHSENGNEQTTPVRTTQKTNGVHEDPKSDSYITLTGHRAGEAAIVYEHGADIEALMPKLRHSDYYTEPRIQELAAKERAEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CX53</accession>
    <name>A0AAD5CX53_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_021931</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006341">
        <property type="protein sequence ID" value="KAI7749564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CX53"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.70">
        <property type="entry name" value="Fucose-specific lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36893:SF1">
        <property type="entry name" value="BULB-TYPE LECTIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36893">
        <property type="entry name" value="OS01G0275950 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89372">
        <property type="entry name" value="Fucose-specific lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="351"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="757"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7749564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="757" mass="85597" checksum="EA0ACCF3F6F19DF1" modified="2024-05-29" version="1" fragment="single">ESKTWVQVNSPYDLLSCVNNNCTKVGIIEPVNDDDDDVDDDQTRTELGQEQESNKIQGQRIKKVKTDERKIGTLGSRNRISVTKMSDDSIWVTGLSGSIYERFWNGIQWIIAPHELPVQAGYAVSVFLIKHIVLALSEAGILYQMQLSENSQPIWVEFPPILDSSNTIRIKSGGVLAFIWMNIGKPPGADVAAIVDAPEVRPQVVFTISSTGDLYEFDPSSKPLWKKHIWSQKSTQDTALTPMTSCTIHQRTRPHSDSLFLLTKGGNLVERRLHQRKWKWVVHGSPKDQQFTSMTLMTIDETYTSSFSLFLATASGSIVEYNISKQEDAPESEGWNAEYCTEDRGPLNCISGVKDETNHDQPTTRSFRRRSGNNKGQENNYYYLVSAASKSKDDAFVGQLSSVNVDFRLRVMYEGRSFFLVTGDGVSYEYLNVENVWLWLKHEHPVGMKGVVGSYNGSLFMINENEDLIVGERGGNEFMWINCSAMKKGRQVVGGPPWDVAPSKARKVTPDDSMFFVGKSGGLLQLTVALKKLKWKDCRNPSNTKIASIVDQEVFRENIIFVVGTDGHLYQYNKVTGLWHGHHQSRHMVLARQPGTAMRGSSQSLSGSLFMISEDGRLIEYYWNPMDGWGWVEHGSPCPDGNVYLRYWDQTLWKWSDCGFPSTVSGFGEKHEGGERCIDQDILVKTRKEYHEETENCDPKVAPTRPIPFTENSVIFELKDGRLAEMRRTENLQWVWWRTIGSPTAHCNAIYWTAAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CYJ7</accession>
    <name>A0AAD5CYJ7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein PALE CRESS, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_008457</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006274">
        <property type="protein sequence ID" value="KAI7749805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CYJ7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009501">
        <property type="term" value="C:amyloplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009509">
        <property type="term" value="C:chromoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009513">
        <property type="term" value="C:etioplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009537">
        <property type="term" value="C:proplastid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071482">
        <property type="term" value="P:cellular response to light stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010239">
        <property type="term" value="P:chloroplast mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009658">
        <property type="term" value="P:chloroplast organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009965">
        <property type="term" value="P:leaf morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034563">
        <property type="entry name" value="PALE_CRESS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37219">
        <property type="entry name" value="PROTEIN PALE CRESS, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37219:SF1">
        <property type="entry name" value="PROTEIN PALE CRESS, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="59"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7749805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="309" mass="36031" checksum="4B5FFDDF0E8062BD" modified="2024-05-29" version="1">MQAQIFRPSFLPPSFSSITTTLSTCSNLSFPNKFPYMVCRCKKLEEEEALAGLPKHFHDEEWQARQREKTKELERLRQEEDEEEERIIDEYREIAMVLKGYPQEDVFKAKKLVSSFIKAAEEIEEKIEEAAERGELNELVLMVIWTRLDLARRDEEKDAIRSLDLLYRRVETEILKRQASPAMRLLNELLIMHDGYDDEGWLKACKKRMIETFPREDPFSLLVPAGFDLDKHHGPLARPAMEADDLLLRIDFIREVDTLLKEVRPTETVESSTEALDPQSVANRLKEQEKKKVIGQVEALLDLAIKLSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CZZ8</accession>
    <name>A0AAD5CZZ8_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_028315</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the lipoxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006302">
        <property type="protein sequence ID" value="KAI7749706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CZZ8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016702">
        <property type="term" value="F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034440">
        <property type="term" value="P:lipid oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031408">
        <property type="term" value="P:oxylipin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.375.10:FF:000001">
        <property type="entry name" value="Lipoxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.375.10">
        <property type="entry name" value="Lipoxygenase-1, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.372.10">
        <property type="entry name" value="Lipoxygenase-1, Domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.60.20">
        <property type="entry name" value="PLAT/LH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000907">
        <property type="entry name" value="LipOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013819">
        <property type="entry name" value="LipOase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036226">
        <property type="entry name" value="LipOase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001246">
        <property type="entry name" value="LipOase_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027433">
        <property type="entry name" value="Lipoxygenase_dom_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001024">
        <property type="entry name" value="PLAT/LH2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036392">
        <property type="entry name" value="PLAT/LH2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11771">
        <property type="entry name" value="LIPOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00305">
        <property type="entry name" value="Lipoxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01477">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00468">
        <property type="entry name" value="PLTLPOXGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00308">
        <property type="entry name" value="LH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49723">
        <property type="entry name" value="Lipase/lipooxygenase domain (PLAT/LH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48484">
        <property type="entry name" value="Lipoxigenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51393">
        <property type="entry name" value="LIPOXYGENASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50095">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="9" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0925">Oxylipin biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PLAT" evidence="12">
        <location>
            <begin position="27"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="Lipoxygenase" evidence="13">
        <location>
            <begin position="153"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="215"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7749706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="384" mass="43850" checksum="DEA62689CC958A2C" modified="2024-05-29" version="1">MFDKTMDGKNMVKGKVVLKKKNVLDVNDVGASVLDGVHEFFGQKISFQLISASHHNDKFTDGKLRGNVGNPAILEDWSSTTLEVKELIYEVEFEWIGLPGALVVKNNHHTEFFLKSLTLQDIPGYGQVHFDCNSWVYPSKFYEKDRVFFSNKAYLPNETPEFLTSYREQELSILRGNGYGELKEWDRVYDYAFYNDLGDPDNDVDYARPVLGGSSEYPYSRRGRTGRPSTKTDPKTESRLPLLKSLDIYVPRDERFGQLKLSDFLGYGFKSIAQFLVSEVLALDITYDEFDSFQDVLKTYEGGIKLPFLEKISKKIPFEFLKVISQTDGEGLAKFPMPQVIEVDKSAWRTDEEFAREMLAGVNPVSIRLLKLNSVFVHVVGENN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D029</accession>
    <name>A0AAD5D029_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_022435</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005882">
        <property type="protein sequence ID" value="KAI7751523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D029"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035251">
        <property type="term" value="F:UDP-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000047">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48047">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48047:SF116">
        <property type="entry name" value="UDP-GLUCOSYL TRANSFERASE 73C7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7751523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="866" mass="97012" checksum="E68905A8E0E18F78" modified="2024-05-29" version="1">MIDMAKLLAKQPNVTVTIVTTPLNAIRYGATLTGLIETGLPVRFLELPFPAAEAGLPEGCESTDKIPSMDLVPNFLAAIGMLQHKLEERYDMIKPVPSCVISDKYMSWTGDFADKYKLPRIMFDGMSCFTQLCYNNLYEHKVFQGLNDSDPFVVPGLPDRIELTRKQLPAEFNPSSVDTSEFRQRARDAEVRAYGVVINSFEELEYEYVKEYKKLRGGKVWCIGPLSLCDSDNLAQSQRGNKAAIDEEQCIKWLDSHKPDSVVYACFGSLVRVNTPQLIELGLGLEASNSPFIWVIKSVHREKEVEDWLAESGFEERVKDRGLIIRGWAPQVLILSHPSVGGFLTHCVVAGTRLWNPCAQFAEQFINEKLVVQVLGIGVGVGADSVVHVGEEDLSGVKVKSESVKNAIEKLHFLVVPLGSPGHYIPTIDLAKLLAQHGVKVTIVTTPVNTIRFGSILDQAIQSGLPISFLELQLPYMKFGLPEGCESLDDLPDLSLGHDLFLAQSSLQQEVEQYIERLNPRPSCILSGTFLLWTCETAKKFQIPRIVFDGMNCFTQMCNHVLYLSKLSFLFNSGSKDVKDFSEKLRKSESEAFGVVINSFYELEPKYADAYQKIKKDKAWCVGPLSLCHKYAAEKVQRGNRSSISENECIKWLDSQENESVIYACLGSISRIEPVQLIELALALESSKKPFIWVVRAGHKTEKIEKWIDEEGFAERTKDRALLIRGWAPQLLVLSHPAVGAFLTHCGWNSALEGISAGVPMVTWPQFQEQFYNEKLLVQVLRIGVSVGVQKVVHLGEEEKSGVVVKSEDLRKAIEMVMVDGKESEDRRKRARDLGKMANEAIEEGGSSYRNMTRLIQDIRNQYNTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D1D3</accession>
    <name>A0AAD5D1D3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PGG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_015121</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005722">
        <property type="protein sequence ID" value="KAI7752213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D1D3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026961">
        <property type="entry name" value="PGG_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24177:SF362">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING DOMAIN, PGG DOMAIN PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24177">
        <property type="entry name" value="CASKIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13962">
        <property type="entry name" value="PGG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="296"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="334"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="375"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="407"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="181"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="PGG" evidence="4">
        <location>
            <begin position="292"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="22"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7752213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="454" mass="51587" checksum="E9212EE16F78B139" modified="2024-05-29" version="1">MNNVRINNNHPHHQQQHTIVPIVPPPVPPPTPPPPNLPRADLLEDVALKIIEDRPELIAKQGLLTDVLLALAQKTHAFKRKKPNLVFRIIKSIFAVFHVKIGPGEKESEALQVLRIIWEKVAIMPKSNIDDILRGPPLKVGNTIKGYPSRVLFLATKVGNTPFIVELIRSYPDLIWKQDDKGKTIFHLAVKRRQAEIYNLLYEIGAMKDLITPMKDKKGNNMLHMVAKSALKTRFQNVSGFALQMQRELLWYKEVEQMIPPQYRQRKNIAGVTPQYLFAKKHAALVTKGESWMKNYMLVATLIATIVFAAAFSLPGGYNQNTGTPFFRKEPALIVFVISDAISLISSSTSVLIFLSILTSSYAELDLLESLPEKLMLGVVTLFLSIVTMMVTFSSSFFVLYDKKLEWVPITITCLAGMPIVLFVKMQFRLLVDIFYSAYRSRSLFKPKKRILYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D1S2</accession>
    <name>A0AAD5D1S2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Gnk2-homologous domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_013427</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006158">
        <property type="protein sequence ID" value="KAI7750410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D1S2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd23509">
        <property type="entry name" value="Gnk2-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.430.20:FF:000002">
        <property type="entry name" value="Cysteine-rich receptor-like protein kinase 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.430.20:FF:000003">
        <property type="entry name" value="Cysteine-rich RLK (RECEPTOR-like protein kinase) 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.430.20">
        <property type="entry name" value="Gnk2 domain, C-X8-C-X2-C motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002902">
        <property type="entry name" value="GNK2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038408">
        <property type="entry name" value="GNK2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32099">
        <property type="entry name" value="CYSTEINE-RICH REPEAT SECRETORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32099:SF99">
        <property type="entry name" value="GNK2-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01657">
        <property type="entry name" value="Stress-antifung"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51473">
        <property type="entry name" value="GNK2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Gnk2-homologous" evidence="5">
        <location>
            <begin position="64"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="Gnk2-homologous" evidence="5">
        <location>
            <begin position="172"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="281"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="355"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7750410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="417" mass="45645" checksum="028124C814410B57" modified="2024-05-29" version="1" fragment="single">MYRLNTTKQTIHYHPTPCVHLISSPTPSPTPKPNMFIPIRTFLLWLTVMFIYSKNTITRAQPPEFLDYKCENAANYTQNSTYQRNLNTTLSALPSTNSGLGFFNFSSGQGTNRVNSIALCRGDINLDVCLSCLNDSIVNLLQLCPNQKEARGYYDYCMVLYSNKEILGNNPIQDGLILYNVQNVTDKDRFNAALRPLLRNLTTDAAAGDSLRKFSSGNTTSGLDFSTIYGLVQCTPDLSQLECSDCVESAISQFMVGYSDKVGGRNLLPMCNFSTFVIPSPPPSSPAPQPNSRASPPVSSSAETVDIGNAESLQYNFSLVKVATNGFSEDNKLGQGGFGAVYKGTLGDGQDIAVKRLARDSGQGDVDGYMAPEYAMHGQFSLKSDVWKNWRSKTLTNVTDPTLKAGSASLRDIIRSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D2F7</accession>
    <name>A0AAD5D2F7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_022789</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CLV3/ESR signal peptide family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005802">
        <property type="protein sequence ID" value="KAI7751837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D2F7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033612">
        <property type="term" value="F:receptor serine/threonine kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044962">
        <property type="entry name" value="CLV3/ESR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36349">
        <property type="entry name" value="PROTEIN CLAVATA 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36349:SF2">
        <property type="entry name" value="PROTEIN CLAVATA 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0221">Differentiation</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="35"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="58"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7751837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="72" mass="7932" checksum="9360E1117A3DE917" modified="2024-05-29" version="1" fragment="single">GFNGEEETLYNGSILNDIISNRKLLLVEAKVLNMGGKSSKEEEEEGWELRAAPLGPDPLHHHGADPQKPRTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D2N5</accession>
    <name>A0AAD5D2N5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_016808</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005560">
        <property type="protein sequence ID" value="KAI7753053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D2N5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018971">
        <property type="entry name" value="DUF1997"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34131">
        <property type="entry name" value="(RAP ANNOTATION RELEASE2) GALACTOSE-BINDING LIKE DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34131:SF3">
        <property type="entry name" value="(RAP ANNOTATION RELEASE2) GALACTOSE-BINDING LIKE DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09366">
        <property type="entry name" value="DUF1997"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042152037" evidence="2">
        <location>
            <begin position="23"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7753053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="414" mass="44115" checksum="AEB74CDE94AD7EFD" modified="2024-05-29" version="1">MANSKIIAIALLVLIVVDLSLAARLSKKTGGKGGGGGGGGHGGGGGGGSGVGEAAGRVSGSGYGSGSGSGYGKGGGGRNGQGQGSGYGSGSGYGSGYGDGDGLTGSLSPHQIPNRATVSSPLFLCFYSLQTQSSPSPLCHPTLPRHQPLYHHRRWSWFGMYEAEELMVVTGAFRFLSDTDQTPNHRHGGWLMVLDGPRVIPRNTFFALTSSNSDDIPKLPLSVSADSSSSSSSSVPKARFVARRTESVSVRPLERPLLEYMSLPASQYSVLDAQRIERIDDNTFRCYVYKFKFFAFEVCPVLLVRVEEQLDGCSINLLSCKLEGSPIVVAQNDKFEASMVNRISCDGNQSSSSMQELTSDTVIEVSIDIPFPFRAIPVRTIESSGTRVLEQILKIMLPRFMAQACNAFYSSVFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D3A6</accession>
    <name>A0AAD5D3A6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Amino acid transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_012800</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005711">
        <property type="protein sequence ID" value="KAI7752287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D3A6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031090">
        <property type="term" value="C:organelle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF289">
        <property type="entry name" value="AMINO ACID TRANSPORTER AVT6C-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="51"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="154"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="183"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="223"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="264"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="302"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="350"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="375"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="409"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="7">
        <location>
            <begin position="24"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7752287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="439" mass="47524" checksum="9A287BABC0EC88C5" modified="2024-05-29" version="1">MKPETTTALLPKTSQQKNEVTGVSSISGAVFNVSTSMIGAGIMSIPATLKVLGIIPGFVVIIIMAVLVEVTVEFLLRYTNYSGEANTYGGVMGESFGKLGSVGLQICIMISNLGALIIYLIIIGDVLSGDQSDGVLHSGILQECFGFHWWNSRAYSVLFIVLFVMLPLLLLPRVESLGHASAVSILLAVVFVVIISGMAVYAMLKGQTQELKLVPDFSDGFSFFNLFTTVPVMATALSCHVMIHPVRAELENQSNMRSAVRISLAISVVIYMAVGFVGYLLFGESIMADMLVNFDQTSNSPGGLVVNAIVRLSYALHLMLVFPVIFYSLRANMDEMIFPQKTLLANDTTRFVSITCVLLAFVYFVAIALPNIWYFFQFMGSTTVACIAFVFPGAIVLRDVHGISTRIDRTMAVIVVILAAITSVIAISTNLYSSFGNTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D581</accession>
    <name>A0AAD5D581_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_015047</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005706">
        <property type="protein sequence ID" value="KAI7752301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D581"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7752301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="137" mass="15569" checksum="5A3A0ACE53ADE3A1" modified="2024-05-29" version="1" fragment="single">MRQSSLFNFHTFMGSLLSNSTLPLIEPCAFLCLFYVLPLWGIPKDEDLNEVNAKASGIVDDIYNSLGEFRTKIVAVYNHQPFGPFQDKDFNLANEQWVVGIMLSVKIMKPGLDLSPLGDVTHQIRRWMRTLPPIISQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D5D8</accession>
    <name>A0AAD5D5D8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">STAS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_021421</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005380">
        <property type="protein sequence ID" value="KAI7753587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D5D8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07042">
        <property type="entry name" value="STAS_SulP_like_sulfate_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.750.24:FF:000002">
        <property type="entry name" value="Sulfate transporter 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.24">
        <property type="entry name" value="STAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011547">
        <property type="entry name" value="SLC26A/SulP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001902">
        <property type="entry name" value="SLC26A/SulP_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11814">
        <property type="entry name" value="SULFATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01740">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00916">
        <property type="entry name" value="Sulfate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52091">
        <property type="entry name" value="SpoIIaa-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50801">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="41"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="77"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="108"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="136"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="170"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="STAS" evidence="8">
        <location>
            <begin position="226"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="386"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7753587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="421" mass="46577" checksum="1532BBEEE1B803F8" modified="2024-05-29" version="1">MYLKQESLDNFFTLIDNLVGNIPQGLPSFSIPKEFGHVKSLISTTFLITGVAILESVGIAKALAAKNGYELDSNQELFGLGVANIFGSFFSAYPATGSFSRSAVNHESGAKTGLSGIIMGLIICSALLFMTPLFEFIPQCALAAIVVSAVIGLVDYEEAMFLWRVDKKDFFLWTVTSATTLFFGIEIGVLVGVGFSLAFVIHESANPHIAVLGRLPGTTVYRNTQQYPEAYTYNGIVIVRIDAPIYFANTSFIKDRLREYEITVDQCTSKRGPEVERISFVVLEMAPITYVDSSAVQALKELYQEYKSRNIQIAIANPNRDVLLTLSNSGFIDLVGKEWCFVRVHDAVQVCLQYVQTSTPSPKTPQVSLHNSTSFSERLNERLNRKDDLTASEMESGERERLLSTDADPMVQPLLSRKSHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D5F0</accession>
    <name>A0AAD5D5F0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_003766</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010003039">
        <property type="protein sequence ID" value="KAI7754576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D5F0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7754576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="92" mass="9908" checksum="57A269043894CEB6" modified="2024-05-29" version="1">MKSFARAMATKASAAVIRAKSACTLMLGMGDDTMHASSVFAMPMVSSKMTGFRGVNSDRSKLLSLRSPSGWNRIPVVEETVNMMDSISSEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D7H2</accession>
    <name>A0AAD5D7H2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein DETOXIFICATION</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Multidrug and toxic compound extrusion protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_023611</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000768">
        <property type="protein sequence ID" value="KAI7755843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D7H2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13132">
        <property type="entry name" value="MATE_eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045069">
        <property type="entry name" value="MATE_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11206">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="99"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="172"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="200"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="234"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="272"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="316"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="351"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="393"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="419"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7755843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="481" mass="52253" checksum="213E82EAD9CC7415" modified="2024-05-29" version="1">MNNKGFTTSQVMDELKHLMSMAIPITAMNQLVFLRAVVSVLFLGRLGSLELAGGSLSLGFTNITGYSVLVGLASGLEPVCSQAYGSNNSNKFDLLSSSLVRMVIILLLVIIPIGVLWINIEQILLFMGQDVDISSMAATYCLYSVPDLLTNSLLQPLRVYLRSQGVTKPMMWCSLIAVVFHVPLNYVLVIGMGLGVGGVAMATVVTNVNMMVLMIGYVWVYGKWRWTWRWTDGIGIWELVRLSVTSCMGICLEWWWYEIVTVLAGYLENPRLAVAGTGILIQTTSLMYTVPMALAGCVSARVGNELGAGRPTKAKLAALVALACAFVVGFINVIWTIVFRDKWSALFTHDHMLQSLVSSVMPIMGLCELGNCPQTTGCGILRGTARPAVGARINLGSFYFVGTPVAVGLAFFYKVGFSGLWFGLLSAQFACVLSVLYSVLVQTDWEGEALRAEKLTNSTSVVEMITECNDAASDNENGFLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DAF1</accession>
    <name>A0AAD5DAF1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_018944</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000534">
        <property type="protein sequence ID" value="KAI7756164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DAF1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026961">
        <property type="entry name" value="PGG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24177:SF475">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING DOMAIN, PGG DOMAIN PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24177">
        <property type="entry name" value="CASKIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13962">
        <property type="entry name" value="PGG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="264"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="302"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="343"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="375"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="667"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="723"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="467"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="802"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7756164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="802" mass="89708" checksum="BCCF10C7F0BE6D8F" modified="2024-05-29" version="1" fragment="single">MDQMSQTKSTSLCLSSQMAGPSNVLMSDSVRKPRSIVYNFTGEKLSDTLSRNQMSPTPLAPYPTHYHWPAPSLYSQMRGSSYTNESLSSESARILDLEYLCLLMTKVSDFVSLKLSGSSNYLLWKAQMVCLLESKNMYGIVDSSFSCPKDSGTGISRQYDSLVRSWIFGSVTEDVLVYVFNLDSAKAVWEKLKSIYDPTVCLQEVKHIKREWSTIPVRFPDNLRNKMDKLEDSDTNHPCNTMPKLYRQRLVKVEQSMKNTAESFSITAALMTAILFAAGIIVQDASNKESEMLTIKVKTPLFIFVISNAISLFASNTALLVFLSILAPGFAEKGFLLGLPRRLFIGLCSFFLCIAAMIVAFSASLFLVFSDQKPWKLHLICGLLFSAIAFSVTLQFPLMVDLFKSAYLRIFGKQRKRHMVRYFEYSEKLRELIVRNRLEAESRFNKERQLATQGISSNGCTVLQLAAETGTELGSLSNYLVSSSAQMICLLESNNICGIVDSTFVGHRDLGTNTMRRYSSVVKGLVLGSVTEDVPANSIEAKLGIEYKENFPDATIKRILFRSPEKRLSFTKSYHGEVELLAKVKVLSSIKPKNNVSLIGIARATQYLDEGLHIGILHRDMKPRNILLDDNFQPKISDFGLSRIFPEVQAYLRTTFARTFGYTAPKYAINATVIAVLIAAVTFAAIFRLPSQYVDKCNQILNGFSSDEGSIAVIQFGMLVIEFWMFVRLALFISLWIVVLQTLIMTVGKCAKKHVKKHVMTVINKLIWLACVSCIVVADGKEKWLAVGVKVVGSVTLASTRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DBX3</accession>
    <name>A0AAD5DBX3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NAD-dependent epimerase/dehydratase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_029179</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAD(P)-dependent epimerase/dehydratase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000057">
        <property type="protein sequence ID" value="KAI7757803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DBX3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43574">
        <property type="entry name" value="EPIMERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="4">
        <location>
            <begin position="48"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7757803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="293" mass="32976" checksum="F47E2C7C3731A8C6" modified="2024-05-29" version="1">MGTTSDTKYGSFTYENLEREPYWPSEKLRVSITGAGGFIASHIARLTENVDHVFNLAADMGGMGFIQSNHSVIMYNNTMISFNMLEAARISGVKRFFYASSACIYPEFKQLETNVSLKESDAWPAEPQDAYGLEKLATEELCRHYTKDFGIECRIGRFHNIYGPFGTWKGVLRLTKSDFREPVNIGSDEMVSMNEMAEIVLSFEDKKLPIHHIPGPEGVRGRNSDNTLIKEKLGWAPTMRLKDGLRITYFWIKEQIEKERGEGFDLSVYGSSKVVGTQAPVQLGSLRAADGKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DCV6</accession>
    <name>A0AAD5DCV6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">REF/SRPP-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_002762</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the REF/SRPP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000115">
        <property type="protein sequence ID" value="KAI7757469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DCV6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008802">
        <property type="entry name" value="REF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33732">
        <property type="entry name" value="REF/SRPP-LIKE PROTEIN OS05G0151300/LOC_OS05G05940"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33732:SF9">
        <property type="entry name" value="REF_SRPP-LIKE PROTEIN OS05G0151300_LOC_OS05G05940"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05755">
        <property type="entry name" value="REF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7757469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="185" mass="20487" checksum="35E4BCF623301065" modified="2024-05-29" version="1" fragment="single">VEIEEERLKYLEFVQLAVLHAVVYASKVYGYAKDNSGPLKPGVETIEDVLKTVVGPAYYKFHDIPVELLKFVDRKVSATTKNLSIEVKKGGVVETASGLAKTAYTKLEPIAEHYAVSAWHTLNQLPLFPKVAKVVVPAAAYYSEKYNQTVRQTAEKGYKVSSYLPLVPTEKIAKAFNSSAPRADH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DHQ3</accession>
    <name>A0AAD5DHQ3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Bet v I/Major latex protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_025112</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the drug/metabolite transporter (DMT) superfamily. Plant drug/metabolite exporter (P-DME) (TC 2.A.7.4) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000044">
        <property type="protein sequence ID" value="KAI7757895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DHQ3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07816">
        <property type="entry name" value="Bet_v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000916">
        <property type="entry name" value="Bet_v_I/MLP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051258">
        <property type="entry name" value="Diverse_Substrate_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42920:SF26">
        <property type="entry name" value="OS03G0707200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42920">
        <property type="entry name" value="OS03G0707200 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00407">
        <property type="entry name" value="Bet_v_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01037">
        <property type="entry name" value="Bet_v_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="131"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="162"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="218"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="470"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="510"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="555"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="587"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="611"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="domain" description="Bet v I/Major latex protein" evidence="9">
        <location>
            <begin position="268"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="72"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="645"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7757895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="645" mass="72212" checksum="5CFA08DF7FB7C01A" modified="2024-05-29" version="1" fragment="single">DEENKSVRFKVTEGDIVDAYKTFAVTIRVDTHEEENVVTWTLDYEKVNEYISNPDRLMELALNGWQKNPSPTGYIRDTSVDDNNNNNDNNDDDVSASEYEVSLKKKHNLNYKSVLTRRKLWRKVVSASTKVRGILLLNVITLLYASNIPVLKEVEMVVDPSAFSAVRFTIAAIPFLPYAWKAREDGQALNSGLELGLWASLGYLMQAFGLLTSDAGRASVITMFTVIVVPLIDGMLGAAIPASTWFGAMMSIVGVGMMECSGSPPCMTLSGTIVKQVTIKSDGDVFHEIFRYRTHHMSEMSPGNIKGVDLHDGEWVTVGSIIVWDFFLDGKTKVVKEVIQAIDKEKKSVCFKVIEGDILEAYKTFLITLHVDTHGDENVVTWTFHFEKLNENVDDPDSLMELCINGHPLRPSYRNARSVDNKFQILETSANADGCACKYEQISKRRMNLNYTFLLTRLAFWPKSSKVRGILLLNVGDLFNFLSALFFGIHMLRTEHISRKTDKTNFLPVVGYEVFVIALSSIIWYLIGGAIGSSIENHPSSWTWEMFLHWLVEFPWIPALYTGVFSTGLCLYIEMTSMRDVSATETAIVYGLEPVWGAGFAWFFLGERWGVSGWFGAALVLGGSLMVQIIGASSRSSSVTFCSTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DIX0</accession>
    <name>A0AAD5DIX0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_001941</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">P-body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat EDC4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000004">
        <property type="protein sequence ID" value="KAI7758390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DIX0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031087">
        <property type="term" value="P:deadenylation-independent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045152">
        <property type="entry name" value="EDC4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15598">
        <property type="entry name" value="ENHANCER OF MRNA-DECAPPING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15598:SF5">
        <property type="entry name" value="ENHANCER OF MRNA-DECAPPING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0853">WD repeat</keyword>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7758390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="157" mass="17668" checksum="6CE0566890D570FA" modified="2024-05-29" version="1" fragment="single">RVTDMCFFAEDVHLLASASMDGRVYVWKILEVPDEDDKPQITEKIAVAVQILGGGEPVHPRVCWHCHKQEVLVVGIGNRVLRFDTTKVGRGKVYSADEPLICHVDKLIDGIQFVGKHDGEITDLSMCQWMTTRLVSASVDGTVSSHRYLLFKLLYRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G157</accession>
    <name>A0AAD5G157_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_004566</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012171">
        <property type="protein sequence ID" value="KAI7724689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G157"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1740.10">
        <property type="entry name" value="VC0467-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003774">
        <property type="entry name" value="AlgH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31984:SF12">
        <property type="entry name" value="THIOREDOXIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31984">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (DUF179)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143456">
        <property type="entry name" value="VC0467-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7724689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="84" mass="9288" checksum="430B6073299C6518" modified="2024-05-29" version="1" fragment="single">MLVATQKLAAVYPFARSKILIVKANQTTGFQGLIINRFISWDSITSLEVGLDSLKQAPLSYGGPVITRELPLVSLTRQSFKHEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G1E2</accession>
    <name>A0AAD5G1E2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SAP30-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_006954</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012149">
        <property type="protein sequence ID" value="KAI7724840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G1E2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012479">
        <property type="entry name" value="SAP30BP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13464:SF0">
        <property type="entry name" value="SAP30-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13464">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR PROTEIN HCNGP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07818">
        <property type="entry name" value="HCNGP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="192"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="357"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="427"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="192"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="387"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="434"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7724840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="446" mass="49672" checksum="B69AC50D58F3492B" modified="2024-05-29" version="1" fragment="single">SGSKLTRIIIIPKFAYSQFNQTLELDTDTNSNTILIIQIYTYNHLIMASKKKESEGIALLSMYGDEEEDDDMEEDDLDNHTNTGNDVVLEENKEQTELLVDNMEQDDDDESDPVNVNHNYNDNDNVNVNDDTTSVLVDKMTTQQVVRSKGSLTIVDYGHDEAALSPEAEEGEIAAAGRVMFGAELQTANGTPQETITTNTQSTPPQSSDQSQPDAYKGNETEYAEAEEAVNVAIEDGRNALDKFLPPPPVNKCSDELQEKITKFLLLKKKTGRSFNSEVRNRKEYRNPDFLLHAVTYQDIDQIGSCFSKDVFDPHGYDKADYYDEIEADIKRELERKEQEKKKNQKIDFLSGGTQSAVAIPTPKVPTQAVPPIAGGGSNAVAATGTREGRPNKKSKWDKVDEDRKHPSGQAHISSVGSAFLSAGTGYTAFAQQRRREEERRSSDRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G1W1</accession>
    <name>A0AAD5G1W1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">chitinase</fullName>
            <ecNumber evidence="2">3.2.1.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_007042</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1-&gt;4)-beta-linkages in chitin and chitodextrins.</text>
            <dbReference type="EC" id="3.2.1.14"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the glycosyl hydrolase 18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.14" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010011926">
        <property type="protein sequence ID" value="KAI7725500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G1W1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008843">
        <property type="term" value="F:endochitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001579">
        <property type="entry name" value="Glyco_hydro_18_chit_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050542">
        <property type="entry name" value="Glycosyl_Hydrlase18_Chitinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45708:SF22">
        <property type="entry name" value="ACIDIC ENDOCHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45708">
        <property type="entry name" value="ENDOCHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01095">
        <property type="entry name" value="GH18_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5" id="KW-0146">Chitin degradation</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8 10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="GH18" evidence="12">
        <location>
            <begin position="1"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7725500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="201" mass="21860" checksum="6B0CFB9D53CE36C2" modified="2024-05-29" version="1">MTIRGEIGSYSLSSPQDAQQVADYLWNTYLGGQSSTRPLGDAVLDGIDFDIEQGTDQFWSDLAKALAAHNSQKKVYLSAAPQCPFPGNGVANQLLPAVREGLFDYVWVQFYNNPQCQYGSGNADALLARWNEWTQLNTNQLFLGVPAAPGAAGSGYIPPDALISQVLPTIKSSPKYGGVMLWDRFFDQQNGYSNAIKGSIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G2E0</accession>
    <name>A0AAD5G2E0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Replication protein A subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">M8C21_019268</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">AA19_3_7</strain>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Component of the replication protein A complex (RPA) required for DNA recombination, repair and replication. The activity of RPA is mediated by single-stranded DNA binding and protein interactions. Probably involved in repair of double-strand DNA breaks (DSBs) induced by genotoxic stresses.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Heterotrimer of RPA1, RPA2 and RPA3 (canonical replication protein A complex).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the replication factor A protein 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011897">
        <property type="protein sequence ID" value="KAI7725592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G2E0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005662">
        <property type="term" value="C:DNA replication factor A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043047">
        <property type="term" value="F:single-stranded telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007140">
        <property type="term" value="P:male meiotic nuclear division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007004">
        <property type="term" value="P:telomere maintenance via telomerase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04474">
        <property type="entry name" value="RPA1_DBD_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04475">
        <property type="entry name" value="RPA1_DBD_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04476">
        <property type="entry name" value="RPA1_DBD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04477">
        <property type="entry name" value="RPA1N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000041">
        <property type="entry name" value="Replication protein A subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000064">
        <property type="entry name" value="Replication protein A subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000090">
        <property type="entry name" value="Replication protein A subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000117">
        <property type="entry name" value="Replication protein A subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047192">
        <property type="entry name" value="Euk_RPA1_DBD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013955">
        <property type="entry name" value="Rep_factor-A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007199">
        <property type="entry name" value="Rep_factor-A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031657">
        <property type="entry name" value="REPA_OB_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004591">
        <property type="entry name" value="Rfa1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00617">
        <property type="entry name" value="rpa1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23273">
        <property type="entry name" value="REPLICATION FACTOR A 1, RFA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23273:SF4">
        <property type="entry name" value="REPLICATION PROTEIN A OB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04057">
        <property type="entry name" value="Rep-A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08646">
        <property type="entry name" value="Rep_fac-A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16900">
        <property type="entry name" value="REPA_OB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3 13" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 13" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="15">
        <location>
            <begin position="773"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="121"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="144"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="179"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU364130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAI7725592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="798" mass="87824" checksum="B02A9DEAC501738D" modified="2024-05-29" version="1">MAINLTAGAIPLLSSGEQPPDDLKPVLQVTEIKLFNTQSESKERYRFSLSDGTYSQQGMLATQLNDRVKSQQVHKGSIVHLTEYSCHRIGDRVIVILISFDVLVDKCDIIGHPKQFAAKLPGNETSSIERSAAPMQPSLNQPATTVTTPNTNVGPPVHARTSEHNAGLHSYGNERSAVPMQSSFNQPTATVTRPNTNVGPPIYTPRSEPNAGLHSYNNAFSHNSVNNRMPPMRPAFNQMPSMYGGIRGPAGKTEAPARIIPIAALNPYQGKWTIKARVTAKSDLRRYSNAKGDGKVFSFDLLDSDGGEIRATCFNTVADQFYNQIEVGKVYYISRGNIKPAQKAFNHLKNDYEITLDQMSTIQPCFDDDNSIPGQQFHFRSIGEVEGMDSNTILDIIGVVYSITPPSTIMTKNQTETQKRSLSLKDMSGRSIDLTLWGNFCNVDGQKIQTMLDSGQFPVIAVKSARVHEYNGKSIGTISSSQLSIEPDLPEAHKLKEWFNSVGRNAPTISMSRESVLQTDKKTLSQIKDEKLGTSEKPDWITVNATILHIRPESFCYTACSIEVGGKKCAKKVVNNGDGKWRCDKCDQTVDECDYRYLLQIQLQDHTGLAWVTAFQESGEDIIGVPAKDLHLIKHEEQDDDKFTEIVRNALFNKFSFKLKVKEESYGDEQRVKSTISKAEKIKFSSNTKALLQEIDKINKKDDPISIAQNHRLNPQTATVGQQTVLPGSHVGQQTALPASHVGHYGSQYGGSVGRMNTGVSSGGGNSGASGECFKCHQMGHWARDCPGVSNVPSYGGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G481</accession>
    <name>A0AAD5G481_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 13">Pectinesterase</fullName>
            <ecNumber evidence="5 13">3.1.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">M8C21_028612</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">AA19_3_7</strain>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11 13">
            <text>[(1-&gt;4)-alpha-D-galacturonosyl methyl ester](n) + n H2O = [(1-&gt;4)-alpha-D-galacturonosyl](n) + n methanol + n H(+)</text>
            <dbReference type="Rhea" id="RHEA:22380"/>
            <dbReference type="Rhea" id="RHEA-COMP:14570"/>
            <dbReference type="Rhea" id="RHEA-COMP:14573"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17790"/>
            <dbReference type="ChEBI" id="CHEBI:140522"/>
            <dbReference type="ChEBI" id="CHEBI:140523"/>
            <dbReference type="EC" id="3.1.1.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 13">Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the C-terminal section; belongs to the pectinesterase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the PMEI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.11" evidence="5 13"/>
    <dbReference type="EMBL" id="JAMZMK010011269">
        <property type="protein sequence ID" value="KAI7728110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G481"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004857">
        <property type="term" value="F:enzyme inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030599">
        <property type="term" value="F:pectinesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042545">
        <property type="term" value="P:cell wall modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045490">
        <property type="term" value="P:pectin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd15798">
        <property type="entry name" value="PMEI-like_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.10:FF:000029">
        <property type="entry name" value="Pectinesterase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.40">
        <property type="entry name" value="Invertase/pectin methylesterase inhibitor family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035513">
        <property type="entry name" value="Invertase/methylesterase_inhib"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033131">
        <property type="entry name" value="Pectinesterase_Asp_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000070">
        <property type="entry name" value="Pectinesterase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006501">
        <property type="entry name" value="Pectinesterase_inhib_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01614">
        <property type="entry name" value="PME_inhib"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31707">
        <property type="entry name" value="PECTINESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01095">
        <property type="entry name" value="Pectinesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04043">
        <property type="entry name" value="PMEI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00856">
        <property type="entry name" value="PMEI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101148">
        <property type="entry name" value="Plant invertase/pectin methylesterase inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00503">
        <property type="entry name" value="PECTINESTERASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13" id="KW-0063">Aspartyl esterase</keyword>
    <keyword evidence="6" id="KW-0134">Cell wall</keyword>
    <keyword evidence="10" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="8 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0964">Secreted</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041771992" description="Pectinesterase" evidence="13">
        <location>
            <begin position="22"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="domain" description="Pectinesterase inhibitor" evidence="14">
        <location>
            <begin position="25"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAI7728110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="525" mass="58270" checksum="EAE0236FD736A4F7" modified="2024-05-29" version="1">MTAKVTLCICVIVTFIFSCSSETTTTTGSINWWCTQTPHHKTCNHHVAVNNISSATISINHFLEMTVKAAIYEARVVRKLAQGIEANYPNVPGKSLWRSCIDYFDGIVFTLNMVLDPTNQPSPLDVHTWLSASITYINVCEKGFETINITTNMLPLISTNLTQLLLNSLAISVAIRGDDSPELHEWNLSDEYKLSNLAMNKPDVVVAKDGSGNFTTVQKAINNVGYRRGRCKRYVIYVKRGIYKEQVVIPYNVSCIMMYGDGVGKTIITGNRHAGGDMLSTSKAGDLKDSATFQVWGRRFIALRIAFRNTAGPQGGQAVAFLSGSDKSALYHCSIEGYQDTLFAFHSKQFYKECKIFGTVDFIFGAAHAVFQDCSIFLRKPLPGGGLVVTANGRKYPNESGGYSLQGCRIKAAGDLKPVIGKYKKAFLGRPWFPYARTVYMQSFLDDLVDPQGWLDSWGFNQTAYCGEYKNYGPGSSTKQRVKWPGHRVITDPNIARRFTVVEFIAGNKWLPASSVPFVPGFEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G4C4</accession>
    <name>A0AAD5G4C4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_022962</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011245">
        <property type="protein sequence ID" value="KAI7728260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G4C4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7728260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="111" mass="11770" checksum="77D30552E22E6D88" modified="2024-05-29" version="1">MEGVPHFPQSIILEGGSIHVDGEVSLFSGSNAQALGGPLDKVLDGQFGNSISRLKLLTVMSQPFCAGGVSFNDSLMWVNPIRDTLAALDLAVGRPLITSWAEFWLGEGLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G512</accession>
    <name>A0AAD5G512_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Dehydrogenase E1 component domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_019005</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011179">
        <property type="protein sequence ID" value="KAI7728637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G512"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004739">
        <property type="term" value="F:pyruvate dehydrogenase (acetyl-transferring) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006086">
        <property type="term" value="P:pyruvate decarboxylation to acetyl-CoA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02000">
        <property type="entry name" value="TPP_E1_PDC_ADC_BCADC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001017">
        <property type="entry name" value="DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050642">
        <property type="entry name" value="PDH_E1_Alpha_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11516:SF60">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE E1 COMPONENT SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11516">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE E1 COMPONENT, ALPHA SUBUNIT BACTERIAL AND ORGANELLAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00676">
        <property type="entry name" value="E1_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Dehydrogenase E1 component" evidence="4">
        <location>
            <begin position="2"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7728637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="242" mass="26500" checksum="C51A2DACE90F51EA" modified="2024-05-29" version="1">MFGFVHLYNGQEAVSSGFIKLLKKEDSVVSTYRDHVHALSKGVPARAVMSELFGKTTGCCRGQGGSMHMFSAEHNVLGGFAFIGEGIPVATGAAFTSKYKREVLKEADCDHVTLAFFGDGTANNGQFFECLNMASLWKLPIIFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPGDLSFEGCYLYLLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G582</accession>
    <name>A0AAD5G582_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_006073</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011277">
        <property type="protein sequence ID" value="KAI7728006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G582"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044794">
        <property type="entry name" value="APRL5/7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47126:SF9">
        <property type="entry name" value="5'-ADENYLYLSULFATE REDUCTASE-LIKE 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47126">
        <property type="entry name" value="5'-ADENYLYLSULFATE REDUCTASE-LIKE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="1">
        <location>
            <begin position="68"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7728006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="269" mass="30620" checksum="EFB555E82FD7A4A7" modified="2024-05-29" version="1" fragment="single">CKDSTESGRILIRIQHSGHLVISTSSSDQHTNEHSVKTILQCPTVIVYSWPVEMSWESVDKVLSSNQTNAYVAILFYASWCPFSRDMRPKFDALSSMYPQIKHVTVEQSSISPIVFSRYGLRGVPSIMITNRTTTMIFLGPKYLYLLAHFYQKATGLKPVMDLTKDPISFPENKSRTAESHNKLKNIVSTEPYLVFSLLFIFSKALLYLYPNILSNITALWHTFIPRLNLAIFGESRQLLGRVLQFVDFKRAFGGLKLTRSARAWPSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G5M3</accession>
    <name>A0AAD5G5M3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">UDP-glucuronate decarboxylase</fullName>
            <ecNumber evidence="4">4.1.1.35</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_026124</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>UDP-alpha-D-glucuronate + H(+) = UDP-alpha-D-apiose + CO2</text>
            <dbReference type="Rhea" id="RHEA:70523"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
            <dbReference type="ChEBI" id="CHEBI:73883"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:70524"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>UDP-alpha-D-glucuronate + H(+) = UDP-alpha-D-xylose + CO2</text>
            <dbReference type="Rhea" id="RHEA:23916"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57632"/>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
            <dbReference type="EC" id="4.1.1.35"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:23917"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NAD(P)-dependent epimerase/dehydratase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.35" evidence="4"/>
    <dbReference type="EMBL" id="JAMZMK010010927">
        <property type="protein sequence ID" value="KAI7729745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G5M3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102765">
        <property type="term" value="F:UDP-D-apiose synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048040">
        <property type="term" value="F:UDP-glucuronate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033352">
        <property type="term" value="P:UDP-D-apiose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05257">
        <property type="entry name" value="Arna_like_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000201">
        <property type="entry name" value="UDP-D-apiose/UDP-D-xylose synthase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045869">
        <property type="entry name" value="Arna-like_SDR_e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050177">
        <property type="entry name" value="Lipid_A_modif_metabolic_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43245">
        <property type="entry name" value="BIFUNCTIONAL POLYMYXIN RESISTANCE PROTEIN ARNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43245:SF40">
        <property type="entry name" value="BIFUNCTIONAL POLYMYXIN RESISTANCE PROTEIN, ARNA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="11">
        <location>
            <begin position="16"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00050895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7729745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="385" mass="43281" checksum="9996294B5EF31BF3" modified="2024-05-29" version="1">MARVDLDGNTIKPMTICMIGAGGFIGSHLCEKLMTETQHTVLAVDVYNDKIKHLLEPDSLPWNGRIQFHRLNIKNDSRLEGLIKCSDLTINLAAICTPADYNTRPLDTIYSNFIDALPVVKYCSENNKRLIHFSTCEVYGKTIGSFLPKDSPLRQDPAYYILKEDTSPCIFGSVEKQRWSYACAKQLIERLIYAEGAENGLEFTIVRPFNWIGPRMDFIPGIDGPSEGVPRVLACFSNNLLRREPLKLVDGGESQRTFIYIKDAIEAVLLMIENPARASGHIFNVGNPNNEVTVRQLAEMMTQVYSKVSGEEAIESPTIDVSSKEFYGEGYDDSDKRIPDMTIINKQLGWNPKTSLWDLLESTLTYQHRTYAEAIKQAIAKPSAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G633</accession>
    <name>A0AAD5G633_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_020937</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010930">
        <property type="protein sequence ID" value="KAI7729732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G633"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000554">
        <property type="entry name" value="protein indeterminate-domain 12-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000131">
        <property type="entry name" value="protein indeterminate-domain 5, chloroplastic-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055187">
        <property type="entry name" value="C2CH-3rd_BIRD-IDD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055185">
        <property type="entry name" value="C2CH-4th_BIRD-IDD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055186">
        <property type="entry name" value="C2H2-2nd_BIRD-IDD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031140">
        <property type="entry name" value="IDD1-16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10593:SF246">
        <property type="entry name" value="PROTEIN INDETERMINATE-DOMAIN 2-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10593">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE RIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22995">
        <property type="entry name" value="C2CH-3rd_BIRD-IDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22992">
        <property type="entry name" value="C2CH-4th_BIRD-IDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22996">
        <property type="entry name" value="C2H2-2nd_BIRD-IDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="70"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="297"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="432"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="7"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="20"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7729732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="529" mass="56941" checksum="21AFCD3A190249C2" modified="2024-05-29" version="1">MVDFENSSSTAMAVSAASTECSSGGNDNRNQPVPETQPVIKKKRNLPGMPDPDAEVIALSPTTLQATNRFVCEICNKGFQRDQNLQLHRRGHNLPWKLKQRDGKEIRKRVYVCPEKTCVHHDPLRALGDLTGIKKHFSRKHGEKKWKCERCSKKYAVQSDWKAHMKTCGTKEYRCDCGTLFSRRDSFITHRAFCDALAQESARSQTLNIQNSPNINSEQHTNVQEAEKMEAAVISPPSCSPPPPLTPSTGVLSPVQSVLSSDLPEKAMGMLQHQKVAVVEMEPVAETCLLSATTATTTTTVSGGSTTSGNNNSNNPSNNPTNSTGVFASIFASSSVPSSQSSSATYSICGVAGADRNTTMEPMSLSLSSSLYHSTTTTQSLFPPPDQALQHRQYLHAQQPALSATALLQKAAQMGATSSNTSFLHALGLTPTPSSASDHYHEPSIGQWSNIQSKQDRNDNSDHLMMGPPAPTTLDFLGLGTGSSSGYSAFLNTIGGSRLSVAGVQFGGLNAGVQNKDWDDSGDKKPTLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G7U0</accession>
    <name>A0AAD5G7U0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">PHD finger protein ING</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_025245</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Component of an histone acetyltransferase complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of an histone acetyltransferase complex. Interacts with H3K4me3 and to a lesser extent with H3K4me2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">The PHD-type zinc finger mediates the binding to H3K4me3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the ING family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010399">
        <property type="protein sequence ID" value="KAI7731712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G7U0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17015">
        <property type="entry name" value="ING_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15505">
        <property type="entry name" value="PHD_ING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000177">
        <property type="entry name" value="PHD finger protein ING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028651">
        <property type="entry name" value="ING_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024610">
        <property type="entry name" value="ING_N_histone-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10333">
        <property type="entry name" value="INHIBITOR OF GROWTH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10333:SF42">
        <property type="entry name" value="INHIBITOR OF GROWTH PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12998">
        <property type="entry name" value="ING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01408">
        <property type="entry name" value="ING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="3 9 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="12">
        <location>
            <begin position="202"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="248"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="251"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="8">
        <location>
            <position position="204"/>
        </location>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="8">
        <location>
            <position position="215"/>
        </location>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="8">
        <location>
            <position position="219"/>
        </location>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="8">
        <location>
            <position position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR628651-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR628651-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7731712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="256" mass="29782" checksum="FDD6499907DC8462" modified="2024-05-29" version="1">MAIARTGVFVDDYLEYASTLPAELQRLLNTIRELDDRSQSMINQTRQQTKNCLDMASQNSYYKGNHEDADMDFEKTKREIEANQDNALNLCTEKVLLARQAYDLIDSHLKRLDEDLHNFAEDLKQEGKLPADEPAILPPLPLVPKNEKRKLPYLTPQSKKVDYRDREWDHRDRDFELMPPPGGFKRDYVPLEMDQPIDPNEPTYCVCHQVSYGDMIACDNENCQGGEWFHYSCVGLTPETRFKGKWYCPTCRELPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G9U1</accession>
    <name>A0AAD5G9U1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein HASTY 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_018964</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009685">
        <property type="protein sequence ID" value="KAI7734620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G9U1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042565">
        <property type="term" value="C:RNA nuclear export complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005049">
        <property type="term" value="F:nuclear export signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006611">
        <property type="term" value="P:protein export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006405">
        <property type="term" value="P:RNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013598">
        <property type="entry name" value="Exportin-1/Importin-b-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045478">
        <property type="entry name" value="Exportin-5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045065">
        <property type="entry name" value="XPO1/5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11223">
        <property type="entry name" value="EXPORTIN 1/5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11223:SF3">
        <property type="entry name" value="EXPORTIN-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19273">
        <property type="entry name" value="Exportin-5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08389">
        <property type="entry name" value="Xpo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exportin-1/Importin-beta-like" evidence="1">
        <location>
            <begin position="106"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="Exportin-5 C-terminal" evidence="2">
        <location>
            <begin position="313"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="Exportin-5 C-terminal" evidence="2">
        <location>
            <begin position="350"/>
            <end position="1036"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7734620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1072" mass="118000" checksum="2EDD38BDCDC9CFAC" modified="2024-05-29" version="1">MNGSDTASEVARAIVAALGWNSSSDDRKAALSYLESVKAGDVRVLASTSFLLVKKDWPSEVRLHAFKLLQHLVRLRWDELSSEERREFAKVSIDLMNEIANSFEEWALKSQTAALVAEIVRREGVSLWQELVPSLISISNSGPTHAEMVCMMLRWLPEDITVHNEDLEGERRRLLLRGLTESLPEILPLLYTLLERHFGAAMTEAGRQQIDIAKQHAATVTATLNAITAYVEWAPLPYLAKYGTIHGCGFLLSSPDFRVHACEFFKLVSSRKRPADADSDYDSAIHSILVILMNVSTDFLNISESGSGIMNDSDFEFAECICESLVSLGSTNLQCITGDNEILSQYLHKLELIRLIASFKPVIAVTKVSERVSMIIKSLLLAPLPSQNLATLESMQLAVDNVVAAVFDGSNDTGSGSDAQLASCRILEGLLQQLLSLNWTEPELVELLGHYFEVLGPFLKYHPDAVGSVINKLFELLNSLPIVMKDPSISGARHARLHICTSFVRLAKTADTSLLPHMKGIADTVSYLQKEGKLLRGEHNLFGETFLIMASAAGPQQQQEVMTWLLEPLSHQWTQLEWQNAYLSDPGGLIKLCGETQFMWSLFHTVTFFEKALKRSGVRKSSVNNNDSTASSVPHPLASHLPWMLPPLLKLLRAIHSLWSPPVTQMLPGEIKAAMAMSDVERTTLLGESNPKMPKGGINFADGIPAHMKDGNAEPNETDIRNWLKGIRDSGYNVLGLSTTVGESFFRCTDVEAVDLALMENISSMEFRHIRQLVHSVLLPLVKNCPSDLWDMWLKRLLYPLLVYSHQALRCSWSALLEEGRAKVPDLCGVIGGPDLKVEVMEEKLLRFLTREICTLLSVLGSPGLNSGLHSEQLPTDSDVLSSSVVGFLLKNKDIAVPLLHMCLDAFTWTDGEAMVKVTSFCGAIVSLAISTNNIELRQFVCKDLFSAIIQGLALESNASSSSNLVGLCCEIFVSFCKQDPAPRQILLSLPCIGPQDLLAFEEALGKTSSPKEQKQLMKSLLLVGTANQLKALAIQRSLNLITNVVARPRSTLPPSDSRTDDGSAIGLAAIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GAI8</accession>
    <name>A0AAD5GAI8_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_017818</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009476">
        <property type="protein sequence ID" value="KAI7735460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GAI8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05506">
        <property type="entry name" value="Bromo_plant1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037377">
        <property type="entry name" value="GTE_bromo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027353">
        <property type="entry name" value="NET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038336">
        <property type="entry name" value="NET_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45926">
        <property type="entry name" value="OSJNBA0053K19.4 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17035">
        <property type="entry name" value="BET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51525">
        <property type="entry name" value="NET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Bromo" evidence="6">
        <location>
            <begin position="124"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="NET" evidence="7">
        <location>
            <begin position="263"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="352"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="28"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="372"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="403"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7735460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="423" mass="46968" checksum="41A4F03CFFAE2B8B" modified="2024-05-29" version="1">MMGKTMADSEGYGSSGRVGTDEPRKKSMNLNSCDSNQQFGRKQLESRLKGELERVRTLRMRIEDGYLPSSDVNSHNAGQRKDRVNVLKDIKNGNVGLKKVGRPTAATGNPVLMKQCDTLLKRLMGHNFGWVFNTPVDVVALKIPDYHTVIKHPMDLGTVKMKLSSGKYADPWGFVADVRLTFSNAMTYNPRGNDVHVMAETLSKFFEVRWKAIEKKLSVSVGTESLVPMRRNVQETEPEGVNIMPPIKKRRTASVGVEIQQEPLMKKKKVMVMSVVEKHKLGSDLEASLADLPATIVDFLKQNSSNGNAAMDEEIEIDIDRLSDETLFKLRKLLDDFLVGKQKNMMEEETCEVEIRNESGPSNSSMQAVKDDMDEEDVDIGDDDLPITSFPPVEIEKDTAAKSSKCSSSSTSSSDSGSSSSDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GCC9</accession>
    <name>A0AAD5GCC9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_020314</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009315">
        <property type="protein sequence ID" value="KAI7736314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GCC9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34212">
        <property type="entry name" value="OS02G0104200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34212:SF1">
        <property type="entry name" value="OS06G0106900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7736314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="222" mass="24669" checksum="048F14580B8E3DB4" modified="2024-05-29" version="1" fragment="single">SRTRGIQLAVGSDAIRLSISNSSNQKSWHPRLPHNLPPRIQSCYTHKDKGMDCGKSPSGVKKMKKKQVKDELDRIKQAEKKKRRLEKALATSAAIISELEKKKQREKEEQRRLDEEGAAIAEAVALQVLIGEDSNDACSQVNYGKQVYRKPSEELGWVMNAHGGSICRWNGYGRGENGSFVRGVEGPYVDEMSAAGLVAARAVSNLQIADDEDINAFVFKRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GDU9</accession>
    <name>A0AAD5GDU9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">U-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_030789</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008750">
        <property type="protein sequence ID" value="KAI7738687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GDU9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057649">
        <property type="entry name" value="PUB62-63_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003613">
        <property type="entry name" value="Ubox_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33644:SF5">
        <property type="entry name" value="U-BOX DOMAIN-CONTAINING PROTEIN 62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33644">
        <property type="entry name" value="U-BOX DOMAIN-CONTAINING PROTEIN 62-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23112">
        <property type="entry name" value="PUB62-63_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04564">
        <property type="entry name" value="U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51698">
        <property type="entry name" value="U_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="U-box" evidence="4">
        <location>
            <begin position="192"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="42"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="68"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="78"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7738687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="374" mass="41214" checksum="8DD21C2FB5C63B41" modified="2024-05-29" version="1">MASDDITLVLSHPSDHHHHHLFYHQPTTTTNHKTTRELTSFIDHHNPHHQPPPYHRGSHHWDVRNGPDSTSSPSGDGSDSDDDDDDNDDMDDDVSRDKHCDVKMAHVSTLGVKEGSVSDVRTAKGIESHLQGQICQYQNAITVADPNGELYYSQLFNGVEGSGAGFKDMLVENGCGFSGRKSVSNSGGSGESLRTILSDPLTGTLMDDAMILPCGHSFGSSGMQHVMRMKACYTCSHPVSESSAAPNLSLRSAVHAFLREEELQSHRASKRRNKLDQDKNNYGDLALVDQIRGRGVQFPFSVSERVLIKGNKRTPPRFVGREAIVTTQCLNGWYVVKTLDNAESVKLQYRSLAKIQNSQPSSVASTKIITPNWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GG75</accession>
    <name>A0AAD5GG75_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Translation machinery-associated protein 22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_016596</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Interacts with the 40S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="2">The SUI1 domain may be involved in RNA binding.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the DENR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008227">
        <property type="protein sequence ID" value="KAI7741267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GG75"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002188">
        <property type="term" value="P:translation reinitiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11607">
        <property type="entry name" value="DENR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.780.10">
        <property type="entry name" value="SUI1-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050318">
        <property type="entry name" value="DENR/SUI1_TIF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046447">
        <property type="entry name" value="DENR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005873">
        <property type="entry name" value="DENR_eukaryotes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048517">
        <property type="entry name" value="DENR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001950">
        <property type="entry name" value="SUI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036877">
        <property type="entry name" value="SUI1_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01159">
        <property type="entry name" value="DRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12789:SF0">
        <property type="entry name" value="DENSITY-REGULATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12789">
        <property type="entry name" value="DENSITY-REGULATED PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21023">
        <property type="entry name" value="DENR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01253">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55159">
        <property type="entry name" value="eIF1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50296">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="SUI1" evidence="4">
        <location>
            <begin position="101"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="59"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="61"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU361273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7741267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="192" mass="21050" checksum="6834034421D38CBB" modified="2024-05-29" version="1">MAEKPQPVQVLYCGVCGLPAEYCEFGPDFEKCKPWLIQHAPNLYPDLIKESNLKEVDGVSDQLQSSSITDGSGPSGSSVPKQEPVKRLPGGKIKKKEKQEVIIEKVTRNKRKSITTIKGLDLFGIKLSDASKKLGKKFATGASVVKGPTEKDQIDVQGDIAYDIVEFITDTWPDVPETAIYFIEDGKKVPAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GGQ8</accession>
    <name>A0AAD5GGQ8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosomal protein eL8/eL30/eS12/Gadd45 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_018414</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL30 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008571">
        <property type="protein sequence ID" value="KAI7739766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GGQ8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000001">
        <property type="entry name" value="60S ribosomal protein L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039109">
        <property type="entry name" value="Ribosomal_eL30-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11449">
        <property type="entry name" value="RIBOSOMAL PROTEIN L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="4">
        <location>
            <begin position="5"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7739766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="91" mass="10126" checksum="4D9F5B69D551550B" modified="2024-05-29" version="1" fragment="single">MTHESINNRLALVMKSGKYTLGYKIALKFLRSSKGNLIIISNNCPPLRKSEIEYYAMLAKVGVLHYNGNNVDLGTAYGKNFRVSCLSIIDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GH11</accession>
    <name>A0AAD5GH11_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CNNM transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_001389</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008523">
        <property type="protein sequence ID" value="KAI7740081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GH11"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030026">
        <property type="term" value="P:intracellular manganese ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010960">
        <property type="term" value="P:magnesium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045095">
        <property type="entry name" value="ACDP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002550">
        <property type="entry name" value="CNNM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12064">
        <property type="entry name" value="METAL TRANSPORTER CNNM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12064:SF97">
        <property type="entry name" value="METAL TRANSPORTER CNNM-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01595">
        <property type="entry name" value="CNNM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51846">
        <property type="entry name" value="CNNM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="31"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="77"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="162"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="188"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="CNNM transmembrane" evidence="5">
        <location>
            <begin position="73"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="357"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="361"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7740081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="498" mass="54066" checksum="7BCAEE1A59BC2955" modified="2024-05-29" version="1" fragment="single">LSTVSLTVFYSSSTAYVISHIYNTTTFNQSIFFHLIIIISITMFLLTLALATGAGAGVSRTSIVTDSNDIEFGNPMWFVYAGVSCVLVLFAGIMSGLTLGLMSLGLVELEILHRSGTSREKKQAAAILPVVQRQHQLLVTLLLCNAAAMEALPIYLDKIFHPAVAVLLSVTFVLIFGEVIPQAISARYGLAVGANFVGLVRVLMVICYPIAYPVGKVLDALIGHSDTLFRRAQLKALVSIHGQEAGKGGELSHDETTIISGALDLTEKTAEEAMTPIESTFSLDVNSKLDWEAFGKILAKGHSRVPVYSGNPKNIIGLLLLEQKQILQVPANMPLYDILNEFQKGGSHMAAVVKVKSKKHPPTDVNDRKLAGKKQSKNSQLTKPLLPKLEESNVIVNIDKTLESTVQHMGPTTKSLRCVTDDEEDGDVIGIITLEDVFEELLQEEIVDETDVYIDVHKRIRVAAAAAASSVARAPSVRKLAAQRSMGVKEEKDNLPRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GHG0</accession>
    <name>A0AAD5GHG0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Dihydroflavonol 4-reductase</fullName>
            <ecNumber evidence="8">1.1.1.219</ecNumber>
            <ecNumber evidence="7">1.1.1.234</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Dihydrokaempferol 4-reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Flavanone 4-reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">M8C21_018512</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">AA19_3_7</strain>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Bifunctional enzyme involved in flavonoid metabolism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>(2S)-flavan-4-ol + NADP(+) = (2S)-flavanone + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11228"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15605"/>
            <dbReference type="ChEBI" id="CHEBI:15606"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.234"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a (2R,3S,4S)-leucoanthocyanidin + NADP(+) = a (2R,3R)-dihydroflavonol + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54444"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:138176"/>
            <dbReference type="ChEBI" id="CHEBI:138188"/>
            <dbReference type="EC" id="1.1.1.219"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pigment biosynthesis; anthocyanin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the NAD(P)-dependent epimerase/dehydratase family. Dihydroflavonol-4-reductase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.219" evidence="8"/>
    <dbReference type="EC" id="1.1.1.234" evidence="7"/>
    <dbReference type="EMBL" id="JAMZMK010007901">
        <property type="protein sequence ID" value="KAI7742780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GHG0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045552">
        <property type="term" value="F:dihydroflavanol 4-reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047890">
        <property type="term" value="F:flavanone 4-reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009813">
        <property type="term" value="P:flavonoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000085">
        <property type="entry name" value="Dihydroflavonol reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050425">
        <property type="entry name" value="NAD(P)_dehydrat-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366:SF852">
        <property type="entry name" value="CINNAMOYL-COA REDUCTASE CAD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366">
        <property type="entry name" value="NAD DEPENDENT EPIMERASE/DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0284">Flavonoid biosynthesis</keyword>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="14">
        <location>
            <begin position="8"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00042087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00042831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAI7742780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="174" mass="18680" checksum="5FEFB82EC40659FF" modified="2024-05-29" version="1">MSGSGETVCVTGASGYIASWVVKFLLARGYTVKASVRDPNDPKKTQHLLKLDGAKERLHLFKANLLEEGSFDAAVEGCDGVFHTASPFYHNVTDPQAELIDPAVKGTLNVLGSCSKASSVKRVVLTSSIAAVAYNGRPRTPDVVVDETWFSDPDLCKETKVVKDALKVQGAKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GII0</accession>
    <name>A0AAD5GII0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">60S ribosomal protein L7a</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_029894</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007635">
        <property type="protein sequence ID" value="KAI7743892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GII0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000003">
        <property type="entry name" value="60S ribosomal protein L7a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050257">
        <property type="entry name" value="eL8/uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004037">
        <property type="entry name" value="Ribosomal_eL8-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018492">
        <property type="entry name" value="Ribosomal_eL8/Nhp2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001921">
        <property type="entry name" value="Ribosomal_eL8_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23105">
        <property type="entry name" value="RIBOSOMAL PROTEIN L7AE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00881">
        <property type="entry name" value="L7ARS6FAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00882">
        <property type="entry name" value="RIBOSOMALL7A"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01082">
        <property type="entry name" value="RIBOSOMAL_L7AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="5">
        <location>
            <begin position="112"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7743892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="254" mass="29034" checksum="304779CD4DC919A2" modified="2024-05-29" version="1" fragment="single">KKGGKPVATKKKTEKVVNPLFEKRPKQFGIGGALPPKKDVHRFVRWPQVVRIQRKRRILKQRLKVPPALNQFTKTLDKNLATNLFKMLLKYRPEDKAAKKERLLKKAQAESEGKTVETKKPIVVKYGLNHVTYLIEQNKAQLVIIAHDVDPIELVVWLPALCRKMEIPYCIVKGKSRLGAIVHQKTAAALCLTTVKNEDKMEFSRILEAIKANFNDKYDEYRKKWGGGIMGSKSQAKTKAKERVLAKEAAQRMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GJM5</accession>
    <name>A0AAD5GJM5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_011939</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the PP2C family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="4"/>
    <dbReference type="EMBL" id="JAMZMK010007955">
        <property type="protein sequence ID" value="KAI7742601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GJM5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009788">
        <property type="term" value="P:negative regulation of abscisic acid-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000065">
        <property type="entry name" value="Protein phosphatase 2C 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000222">
        <property type="entry name" value="PP2C_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01032">
        <property type="entry name" value="PPM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 10" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="11">
        <location>
            <begin position="129"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7742601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="424" mass="46151" checksum="8B1D44AC188C95E6" modified="2024-05-29" version="1">MKMADICCGVVSENEASSVVACESNSQATTRRKRMELRRVNFVSGVPSLEPDNNGVKQPRLSVSPASFLRCECNTATVSSCNCTESIHRQKQDDDVGKGVIIKAATPLVAAILKPLGSALFVDGEKAPKFGVASVCGRRRDMEDAVAIHPSFLTINDDQTSNMHYFAVYDGHGCSHVATRCKDRLHLLVKEELDKKVDTSEWRKTMERSFKRMDKEVIDWNKGTLGGNCRCELQAPDSDAVGSTAVVAITTPDKIIVANCGDSRAVLCRNGKAIPLSSDHKPDRPDELNRIQAAGGRVIYWEGARVLGVLAMSRAIGDNYLKPYVSSEPEVTITERTSEDECLIIASDGLWDVVSNDTACGVARMCLKGKGPSASLKSKVENKEPGLEKWDKACSDASMLLTKLALARRSSDNVSVVVIDLRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GKN8</accession>
    <name>A0AAD5GKN8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA mismatch repair proteins mutS family domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_008298</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006960">
        <property type="protein sequence ID" value="KAI7746485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GKN8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03243">
        <property type="entry name" value="ABC_MutS_homologs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001188">
        <property type="entry name" value="DNA mismatch repair protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000432">
        <property type="entry name" value="DNA_mismatch_repair_MutS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016151">
        <property type="entry name" value="DNA_mismatch_repair_MutS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000305">
        <property type="entry name" value="GIY-YIG_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035901">
        <property type="entry name" value="GIY-YIG_endonuc_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053276">
        <property type="entry name" value="MtDNA_mismatch_repair_MutS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48448">
        <property type="entry name" value="MUTL PROTEIN ISOFORM 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48448:SF1">
        <property type="entry name" value="MUTL PROTEIN ISOFORM 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01541">
        <property type="entry name" value="GIY-YIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00488">
        <property type="entry name" value="MutS_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00534">
        <property type="entry name" value="MUTSac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55271">
        <property type="entry name" value="DNA repair protein MutS, domain I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82771">
        <property type="entry name" value="GIY-YIG endonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00486">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair proteins mutS family" evidence="5">
        <location>
            <begin position="758"/>
            <end position="774"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS00486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7746485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="1048" mass="116254" checksum="08D60178A59A50E0" modified="2024-05-29" version="1">MSASVAISVPWRCSSSSPIPYCFSSSSIIIFSFSSLQPKQVYCFKEKKSTSTKSAKKLRELKDVIVEKDYPHIMWWKEKMQTCVKTSSVQLVTRLVGDFYEAIGFDACILVEYAGLNPCGGLRSDSIPKAGCPVVCIVEEVQGPSQARSRKSRFISGHAHPGSPYVFGLVEDDCDLEFPEPMPVVGVSRSAKGYCMVSVLETMKTFSSEDGLTEEALVTKLRTSSWREFGEGGLLWAECNGRHFEWLEGDMLDEILFRVKELYGLDDNVTFRNVTVASENRPRPLHLGTASQIGAIQTEGIPFLLKYLLPSNCTGLPAMYVRDLLLNPPAYAIASTIQDICKLMSNVSCTIPEFTCITPSKLVKLLELRETNHVEFCKIKSVLDEILQLYSNSELNEILRLLMDPTWVATGLKIDMETLVKECESVSHRIAEIISLDGESNQKMSSHVNIPNEFFEEMESSWKGRVKRIHLIEAYEEVDKAAEALSLAATEDFLPVVLRVRAATASFGGPRGEITYAREHKAVWFKGRRFTPAVWAGTPGEEQIKQLRPSVDARGRKVGEEWFTTPKVEDALTRYHEACANAKTMVLDLLRDLSAELQAKVNVLVFASMLLVIAKALFAHVSEGRRRKWVFPTLINSFDSQEKGQTNGYHEMQITGLSPYWFDAAEGSGVRNTIDMKSMFLLTGPNGGGKSSLLRSICAAALFGICGFMVPAESATIPQFDSIMLHMKSYDSPADGKSSFQIEMSELRSIIVGATSKSLVLVDEICRGTETAKGTCIAASIVETLDSIGCLGIVSTHLHDIFKLPLTTRNTVFKAMGSEYVDGQTKPTWKLIDGICRESLAFETAKREGVPEAIIQRAEELYISMRTTDEKDLSSEKGSHKTDNHPTVINLPSNLSFVESTDEMQKFLKEVETAVSMICQRGLIEVCKLRSTSEGVIRCVLIAPRQQPPPSAIGASSVYVILRPDNRLYVGETDDLEGRVRAHRSKQGMQNASFLYFLVSGKSVACQLETLLINQLPKHGFQLTNIADGKHRNFGTCDASIENVTLHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GKU2</accession>
    <name>A0AAD5GKU2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DC1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_033071</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007464">
        <property type="protein sequence ID" value="KAI7744669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GKU2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004146">
        <property type="entry name" value="DC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053192">
        <property type="entry name" value="Vacuole_Formation_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32410">
        <property type="entry name" value="CYSTEINE/HISTIDINE-RICH C1 DOMAIN FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32410:SF161">
        <property type="entry name" value="DC1, ZINC FINGER, RING_FYVE_PHD-TYPE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03107">
        <property type="entry name" value="C1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="DC1" evidence="2">
        <location>
            <begin position="85"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7744669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="160" mass="18571" checksum="B51B703C82568534" modified="2024-05-29" version="1" fragment="single">MSILASRGTSKTFKSYEDSQHPDLLHLPFLDPSYSMLKHLFFKEDIGSSTYKTQNNTIHQHPLILVDARCTDITSIPTSSRIKPISYHDPMKRIELLCDVCVRPIMSGPIYMCSNEEKDHCNFVLHEWCSKLPTELKHHFHHPQQTLILHSKIPVHYECC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GM31</accession>
    <name>A0AAD5GM31_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_015774</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006636">
        <property type="protein sequence ID" value="KAI7747925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GM31"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7747925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="96" mass="10722" checksum="70E2666326489F6F" modified="2024-05-29" version="1" fragment="single">FVSQPNLWSGSGVRLERYKETGVEPRYAAYKMLAASADAASLAVFFRTSSSFCSVVAWWMVAVAVTGKGSTAMVRIKEDTRRLATMREIQFSNVYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-06-18" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GM90</accession>
    <name>A0AAD5GM90_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">E3 ubiquitin-protein ligase LIN-1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_016898</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007447">
        <property type="protein sequence ID" value="KAI7744763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055566">
        <property type="entry name" value="ARM_LIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056514">
        <property type="entry name" value="ARM_LIN_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056512">
        <property type="entry name" value="LIN_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35549">
        <property type="entry name" value="OS04G0584500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35549:SF2">
        <property type="entry name" value="TRANSDUCIN_WD40 REPEAT-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23568">
        <property type="entry name" value="ARM_LIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23654">
        <property type="entry name" value="ARM_LIN_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23628">
        <property type="entry name" value="ARM_LIN_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Putative E3 ubiquitin-protein ligase LIN N-terminal" evidence="1">
        <location>
            <begin position="62"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="Putative E3 ubiquitin-protein ligase LIN ARM repeats" evidence="3">
        <location>
            <begin position="414"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="domain" description="Putative E3 ubiquitin-protein ligase LIN ARM-like" evidence="2">
        <location>
            <begin position="589"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF23568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7744763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1254" mass="141754" checksum="367DD289671E9BEF" modified="2024-05-29" version="1" fragment="single">VAQQFFLYFTTQHNTCSSSSSSSTQHSYLNSTTTSMASTSSSSSPTHHHNHIPINPDLLIHLITSINHHMHVLISNSITRKLLQTKCTSNLVIHTHNSFDFSQQSILSNFCYGIKKAEEAIETNNNMYIEQAENMLQAPASLNENGVSCGVSNGYLVCCSYFYLGMLRKVQGDDMQVAVNVXXXXVVAPWLVRADLAPEMYRVVVQVLGLRLDLGEGFDGVREGAMRFKAWLMYYQVMCYGGSPLLLKHKSHEIGNTKACSTSSSFSYQHHHQNFVKVHPIDHQQNTNAAIVERSVSKKVYETSGLKRLQDLIMEDQSEVQSLYSGSSGFGDDDFEEYADEFDRSLRALHVAAEQPETTISTSKSSEFWNCLEDDEEKPAWENYQISQWKYSQEISNMRDEIIFSGENSYRVEQAEIIQKVISKLCFSEKLGQRFNDYFDDDDDDDDSTTTEITAVYEMLANKPGLKYSLLKDVILDQLLMALSTSKEQGVIRASVSILSSIISSNRSVIDDIKKKGLSLHDLANALKRDVYEAVVLIYLINPSPNEIKTLELMPTLVKIICSSSGFRSTLKSVLVTPHSASLMIVQVLVTAFDYSTNAAHLAAITSPKVISCLVNVPRYGNLEEGVSLAAILVKCMRFDGQCRYHISQLNPISPFVSLILSNHKDAILAGLEFYHELLRMPRSSSVNLLQQLREKGGIDIMCVLLLAIQQTQQEYKLLAASLLLQLDVLEESVDMILNRDVAIETLVEALTCEEDSDSQQLAAFILSNIGGTYAWTGEPYTIAWLVKKVGLNSVLHKNIVKNIDWSDDTLQENETEVWSGKVARHIIKLGNPVFYALKKGLKSKNKRTSRDCLTTIAWIGCELVKGPDDLRCSACDILLNAIEQYVHPGMELEERLLACLCIYNYTFGRGMQKLTQLSEGVRESLRRLSSITWMAEELLKVADYYMPNKWVWDIKGQTMXXXXDFKEHKKAIWQMFERKIECVEVITTKDSIQSLETCGEMIFAVTHSHKVKVFDSSRKVKDIFKNKHVKCIKANQGKVYIGCMDSSIQELIIGNNRQQEIKAPSKSWRMQNKPITSISLYKDWLYSASTCIDGSRIKDWRRQSMPQVSIVLEKGANIVAMSIVEDFIYLNTSCSSSNVEIWLRGTQHKVGRLSAGSKITSMLTANDMILCGTESGLIKVTFAYFIRVGYRFDMQWTQCCIWLSIYHAVNIYKEKWAAHNVHELHTTIIYLNTIHLHLPSLFSHDKKLGETRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GMQ4</accession>
    <name>A0AAD5GMQ4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phytosulfokine</fullName>
        </recommendedName>
        <component>
            <recommendedName>
                <fullName evidence="9">Phytosulfokine-alpha</fullName>
                <shortName evidence="9">PSK-alpha</shortName>
                <shortName evidence="9">Phytosulfokine-a</shortName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName evidence="9">Phytosulfokine-beta</fullName>
                <shortName evidence="9">PSK-beta</shortName>
                <shortName evidence="9">Phytosulfokine-b</shortName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_014211</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Promotes plant cell differentiation, organogenesis and somatic embryogenesis as well as cell proliferation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="9">PSK-alpha is produced by endopeptidase digestion. PSK-beta is produced from PSK-alpha by exopeptidase digestion.</text>
    </comment>
    <comment type="PTM">
        <text evidence="9">Sulfation is important for activity and for the binding to a putative membrane receptor.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the phytosulfokine family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006927">
        <property type="protein sequence ID" value="KAI7746649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GMQ4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008283">
        <property type="term" value="P:cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009438">
        <property type="entry name" value="Phytosulfokine"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33285:SF33">
        <property type="entry name" value="PHYTOSULFOKINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33285">
        <property type="entry name" value="PHYTOSULFOKINES 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06404">
        <property type="entry name" value="PSK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7 9" id="KW-0221">Differentiation</keyword>
    <keyword evidence="8 9" id="KW-0339">Growth factor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 9" id="KW-0732">Signal</keyword>
    <keyword evidence="5 9" id="KW-0765">Sulfation</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041773960" description="Phytosulfokine" evidence="9">
        <location>
            <begin position="21"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU368031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7746649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="90" mass="10125" checksum="D8C67A2CD681AB69" modified="2024-05-29" version="1">MKHTFRCAVSFIFLLSLTSARFLHTKPGDNMLTLDESINISGSEHSLVDLDSFINEGMECGSGDEECLKRRVLAVADAHLDYIYTQHHKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-04-02" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GPB4</accession>
    <name>A0AAD5GPB4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mediator complex subunit 15 KIX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_012664</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006860">
        <property type="protein sequence ID" value="KAI7747013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.246.20:FF:000003">
        <property type="entry name" value="Mediator of RNA polymerase II transcription subunit 15a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.20">
        <property type="entry name" value="Coactivator CBP, KIX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036529">
        <property type="entry name" value="KIX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036546">
        <property type="entry name" value="MED15_KIX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044661">
        <property type="entry name" value="MED15a/b/c-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33137">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 15A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33137:SF4">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 15A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16987">
        <property type="entry name" value="KIX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47040">
        <property type="entry name" value="Kix domain of CBP (creb binding protein)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mediator complex subunit 15 KIX" evidence="4">
        <location>
            <begin position="24"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="96"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="198"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="276"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="384"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="544"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="664"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="96"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="140"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="151"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="215"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="277"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="316"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="388"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="398"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="419"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="475"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="668"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7747013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="724" mass="81424" checksum="D6F5F8A5001E7BA6" modified="2024-05-29" version="1" fragment="single">MESNNWRPTQGASGAVVDPSMESVDWRAQLQADSRQRIVNKIMDTLKRHLPFSGNEGLQELKKIAVRFEEKIYTAATSQSDYLRKISLKMLTMENRSQNPMPDGMQSSSAANSVNPSDPGSQVMQQGQQLPISAPSNNIQSGQQMMSQGMHSNIGSAGMQGSNLSSALPPGNGLQQSTMANVAGQNSNLQNMQNMSGVQQNNVGNTMGPGNFANQRQQMQGRQQLAPQQQQQQXXXXXXXXXXXXXXXXXXXXXXQQQQQQTQNSHYLYQQKLHQMAKQTLQQPSMQSHLPQQNLLQPGHPQQSVMQPTMMTSSLSSGVPNQQPVVPQSSQPLMNHQQSVLRQRPQQVQPTQQQVQSTQQNLMGQQANAANMQQNQLIGQQNNYSDMQQQQQQQQQQRLMGQQNKLSSVQQPQQQLIGQHNNLSSMQQQQSGQQQQLHGSQSGNSNMQTNQHSMQQMLQSSMPMQQQNHQSSAPLLSTQVQSSLTQLQQHQGMPHMQSQSGQLQQQVGMQQQPNLLQRDMQQRLPTSGVFQQQNVIDQQKQLFQQQRAMPEASSTSLDSTAQTGNSNGGDWQEEVYQKIKAMKDLYLLDLTDMHQKILGKLQQYDSLPQQPKNDQLDKLKMFKSMLERYIQLLQLPNKHNILANYKDKLGTYEKQIIQVINSHRRKPGGPQQQGQALPPAHMQSMQQSQQTHSQLTQVQSHDTQMNMQGSMAAMQPNNMGNLQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GS16</accession>
    <name>A0AAD5GS16_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">rRNA methyltransferase 2, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_021308</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA methyltransferase RlmE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005949">
        <property type="protein sequence ID" value="KAI7751239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GS16"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008650">
        <property type="term" value="F:rRNA (uridine-2'-O-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000212">
        <property type="entry name" value="Ribosomal RNA large subunit methyltransferase E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01547">
        <property type="entry name" value="RNA_methyltr_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050082">
        <property type="entry name" value="RNA_methyltr_RlmE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002877">
        <property type="entry name" value="RNA_MeTrfase_FtsJ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015507">
        <property type="entry name" value="rRNA-MeTfrase_E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10920">
        <property type="entry name" value="RIBOSOMAL RNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10920:SF18">
        <property type="entry name" value="RRNA METHYLTRANSFERASE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01728">
        <property type="entry name" value="FtsJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005461">
        <property type="entry name" value="23S_rRNA_mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="5 7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ribosomal RNA methyltransferase FtsJ" evidence="8">
        <location>
            <begin position="1"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="167"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00041184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR005461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7751239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="211" mass="22918" checksum="EA55156AD82FE6E6" modified="2024-05-29" version="1" fragment="single">YVARSAFKLVQIQKQYKLITPGSSVLDLGCAPGAWLQVACQSLGPLKTGGVVVGVDLKKVKVPSTHCDSRVSTICADVMNLPKDEFLNFSPEKKGFSVVLSDMCPLVSGITTRDAALSMELGMRALDLALGGSKLPVVPLEDGHTETQLNKEDDVGILKTGGHLIVKLLESEDTKELIKICKPLFRKSSWLRPKATRSNSREIYLICQGLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GT23</accession>
    <name>A0AAD5GT23_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FBD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_006035</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010003865">
        <property type="protein sequence ID" value="KAI7754347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GT23"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006566">
        <property type="entry name" value="FBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055411">
        <property type="entry name" value="LRR_FXL15/At3g58940/PEG3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31639">
        <property type="entry name" value="F-BOX PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31639:SF315">
        <property type="entry name" value="LEUCINE-RICH REPEAT DOMAIN SUPERFAMILY, F-BOX-LIKE DOMAIN SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24758">
        <property type="entry name" value="LRR_At5g56370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00579">
        <property type="entry name" value="FBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FBD" evidence="1">
        <location>
            <begin position="253"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7754347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="333" mass="37931" checksum="84EF590F821BEE97" modified="2024-05-29" version="1">MVTVSSNHICTQLEKFKLVNAIFHVLLFHNGPHTLVFACPIVRLHMESEFAQITSYLARGNKVKYLVFGNDNRSYKLPIPFFSLQGLERVHLETCTFEPPLTFKGFSLLTSITLFNVDVSAKMLKRFISMCPLLQYLYLNELQKSVDSVAGGNEFTFVDILPCVPLIQTLDISKYYTKYLYAGGMPHKLPASLVHLKGLYLYVCLMEENEISSALCMMRSSPVLEVIVFKMYDNYKLHVRQTLPNFLDPKGHPDLKLDRLETLVIEMFSNLSVEMDFVKLIMAKSPTLKKVRIELNGNVSVKEELEMLRELVLNPIPRASPSAKLSVVRPNTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GTI5</accession>
    <name>A0AAD5GTI5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_001574</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010005788">
        <property type="protein sequence ID" value="KAI7751931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GTI5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14016">
        <property type="entry name" value="STKc_CK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000222">
        <property type="entry name" value="casein kinase 1-like protein HD16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055900">
        <property type="entry name" value="DUF7477"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24289">
        <property type="entry name" value="DUF7477"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="36"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7751931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="467" mass="52007" checksum="BDEAED475C524EE6" modified="2024-05-29" version="1" fragment="single">MGDSGGLSANKVTGQEEEGNTAPFPERVQVGGSPLYKVERKLGKGGFGQVFVGRRVSGGTDRISGPGATEVALKFEHRNSKGCSYGPPYEWQVYNTLGGSHGVPKVHYKGKQGDYYVMVMDMLGPSLWDVWNSSGQSMSSEMVACIAVESLSILEKMHSRGYVHGDVKPENFLLGQPSTPQEKKLFLVDLGLATKWRESASGQHVDYDQRPDMFRGTVRYASVHAHLGRTASRRDDLESLAYTLVFLHRGRLPWQGYQGDNKSYLVCKKKMATSPEMLCSFCPAPLKHFLEIVVNMKFDEEPNYSKLISLFEGLLGPNPAIRPINTDGAQKIIFQVGQKRGRLNLDEEDDGQPKKKIRMGVPATQWISVYNARQPMKQRYHYNVADARLAQHVERGNADGLLISSVSSCSNLWALIMDAGTGFSNQVYELSPYFLHKEWIMEQWEKNYYISAIAGANNGSSLVVMSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GTJ7</accession>
    <name>A0AAD5GTJ7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">beta-aspartyl-peptidase</fullName>
            <ecNumber evidence="3">3.4.19.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_005309</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleavage of a beta-linked Asp residue from the N-terminus of a polypeptide.</text>
            <dbReference type="EC" id="3.4.19.5"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterotetramer of two alpha and two beta chains arranged as a dimer of alpha/beta heterodimers.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.5" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010002740">
        <property type="protein sequence ID" value="KAI7754690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GTJ7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004067">
        <property type="term" value="F:asparaginase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008798">
        <property type="term" value="F:beta-aspartyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04701">
        <property type="entry name" value="Asparaginase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.30:FF:000001">
        <property type="entry name" value="Isoaspartyl peptidase/L-asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.30">
        <property type="entry name" value="(Glycosyl)asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000246">
        <property type="entry name" value="Peptidase_T2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10188:SF13">
        <property type="entry name" value="ISOASPARTYL PEPTIDASE_L-ASPARAGINASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10188">
        <property type="entry name" value="L-ASPARAGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01112">
        <property type="entry name" value="Asparaginase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="7">
        <location>
            <position position="196"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="224"/>
            <end position="227"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="246"/>
            <end position="249"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Cleavage; by autolysis" evidence="9">
        <location>
            <begin position="195"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR600246-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR600246-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR600246-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7754690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="326" mass="34414" checksum="986F39EC6E4647AC" modified="2024-05-29" version="1">MVGWAIAVHGGAGVDPTLPTERQELAKQLLTRCLNLGITALKSSVSAIDVVELVVRELETDPTFNSGRGSALTEKGTVEMEASIMDGSGRRCGAVSGLTTVKNPVSLARLVMDKSPHSYLGFSGAEDFAKQMGVEREENDYFVTEDNIGMLKIAQEANSIVFDYRIPTNGYESCGVEVENPIRMNGLPISVYAPETVGCVAVDSQGHCAAATSTGGLMNKRTGRIGDSPLIGAGTYACDVCGVSCTGEGEAIIRGTLAREVAAVMEYKGLGLQEAVDFVIKERLDEGQAGLIAVSKTGEVACGYNCVGMFRGCATEDGFMEVAIWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GUL0</accession>
    <name>A0AAD5GUL0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_000065</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005584">
        <property type="protein sequence ID" value="KAI7752906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GUL0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057135">
        <property type="entry name" value="At4g27190-like_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050905">
        <property type="entry name" value="Plant_NBS-LRR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463:SF96">
        <property type="entry name" value="LEUCINE-RICH REPEAT DOMAIN, L DOMAIN-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23247">
        <property type="entry name" value="LRR_RPS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0611">Plant defense</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NB-ARC" evidence="7">
        <location>
            <begin position="140"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="9">
        <location>
            <begin position="450"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein At4g27190-like leucine-rich repeats" evidence="8">
        <location>
            <begin position="668"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="33"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF23247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7752906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="843" mass="95412" checksum="223149CD4B34F141" modified="2024-05-29" version="1" fragment="single">MDFVTPFIGPFVESLTARVKKHFRFFVSSTEYVEDMKNKMDQLEDTSKDVQDDLEKAVLHAEVVPAHAQPWLKEVERLKIRRNSIEAIGCFNVIKRYKAGKKSCKIMEEIEDLENPGPSTSAIISMGTQNNFESRDSIFNDALQALRSNKESETMVALCGMAGVGKTTMMEQLEKAVADLRMFDWVVKVVIGENSDPYSLQQDVAQLIGERLNETAISARADSLRKIFEVKSQQGRKKNLVIMDDLWKKVDLTVVGLSPLPNGFKLSFTSRFEDVCNKMDVTTDLIFKIRVLSDPDAKALFFTRVGPSPSDGDEDELRRIGEAIVKKCCGLPIAIVTIAENLKGKTKEAWNEALSNLQHGDLQDLYSIVHKIFEMSYNNLEKEDDKAIFILSGLFPDDFNIPVENLMVYGWGLKLFTKFPEDMHRRMKTLEVGSYENMGTPMFPITLEHSTSLRTLSLRSCSLTGDISFIGSLSNLETLSLADCNIRRLPSTIGKLKKLRLLNLTGCVDLDIDHGVFQNLDSLEELYLRSSKGSPVRFTHANCDEFEKLSQLRYLHVSKCAELQYLFTVDVANGLTKLERLTISECPVLKTLIGENDGVGVVTLKKLNFMSLEDLPEMVSLCDNVVELPELVELKLAGLPNFTSIYPDSHNTDSMQPLFNTEGAVPKLENLDICLMENLKQIWPCQISTSEKNEVSMLRQITVGECDSLINLFPINPLPLLNHLEEVVVKKCGSIEVLFNIDFESVCEMGKHSSSRLRRIEVERLGKLKELWRMKSVNESDILVNNFKEVQTIHIEWCDNFTDIFTPTTTKFDLRALTDYVSKDNSDDLEEREKGNKMIESNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BJT6</accession>
    <name>A0AAD5BJT6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Translocon-associated protein subunit alpha</fullName>
            <shortName evidence="7">TRAP-alpha</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Signal sequence receptor subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_017271</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">TRAP proteins are part of a complex whose function is to bind calcium to the ER membrane and thereby regulate the retention of ER resident proteins. May be involved in the recycling of the translocation apparatus after completion of the translocation process or may function as a membrane-bound chaperone facilitating folding of translocated proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Heterotetramer of TRAP-alpha, TRAP-beta, TRAP-delta and TRAP-gamma.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Endoplasmic reticulum membrane</location>
            <topology evidence="1 7">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">Shows a remarkable charge distribution with the N-terminus being highly negatively charged, and the cytoplasmic C-terminus positively charged.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the TRAP-alpha family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012222">
        <property type="protein sequence ID" value="KAI7724428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BJT6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005595">
        <property type="entry name" value="TRAP_alpha"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12924:SF0">
        <property type="entry name" value="TRANSLOCON-ASSOCIATED PROTEIN SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12924">
        <property type="entry name" value="TRANSLOCON-ASSOCIATED PROTEIN, ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03896">
        <property type="entry name" value="TRAP_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="4 7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7 8" id="KW-0732">Signal</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042061304" description="Translocon-associated protein subunit alpha" evidence="8">
        <location>
            <begin position="28"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="194"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU368074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7724428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="284" mass="30960" checksum="63DE2D89D2B873D6" modified="2024-05-29" version="1">MAKIRAFFILSLLVLLSSSFFLQVARSQSEPDSEADAELVAETVKEGSHVDDDVQDIGFGNYNPAPGVETVSVFPKNFAKLVVAGQETELLVGLKNEGEHSVKVIAVRASVHLPYDHRMLVQNLSTVAFNDASVPASVQATFPYTFAVSKFLQPGTFDLVGTIVYEIDQVPYQNVFYNGTIEVTEAGGLVSVETVFLVSLGLALLVLFGLWIRTQLQSLSKKTKKITKVEVGTRTVDASMDEWLQASEVEVGTVFAYIRAGIGEKSGHISPFQRLGVQNDIEAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BK03</accession>
    <name>A0AAD5BK03_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF547 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_025684</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012218">
        <property type="protein sequence ID" value="KAI7724456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BK03"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006869">
        <property type="entry name" value="DUF547"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46248">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46248:SF9">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04784">
        <property type="entry name" value="DUF547"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF547" evidence="3">
        <location>
            <begin position="265"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="46"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="91"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="46"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="111"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7724456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="468" mass="52791" checksum="05A4711F2104A669" modified="2024-05-29" version="1" fragment="single">TLELLAEVAVLEEEVVRLEEQVVHFRQSLYQEAVYISSSKKNLENSLDFQEPTNGENKASKLSPQVETNPSAPIENSPVVSVLVGKDNQPSSVIKNKYRSPNPKSQTVDTLSRRSPLDSKSPRKRLAPKKLQTNQSRSLEAANVDRKTNLIQEKGLLSSVDDDPNKISESILKCLMNIFVRMSSTRSKSMTEMLPSLTTIETVKETEFKDPYNICFEFEKRDVGAYKNLHAIEPTSINKNRTANSVFLIQRLKLLLGKLASVSLTSLTHQEKLAFWINIYNSCMMNAFLEHGIPDSPEMVVQLMQKATINVGGYHLSAISIEHFILRLPYHSKYTFAKGVKNDEINARSVFGLELSEPLVTFALSCGSWSSPAVRIYTGSQVENELETAKKDYLQAAVAISTTKKMVAIPKLLDWYMLDFAKDMDSLLDWVCLQLPNETGKQAMKCLERNKSVPLSHCLRIIPYEFQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BKL6</accession>
    <name>A0AAD5BKL6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNL-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_026382</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012102">
        <property type="protein sequence ID" value="KAI7725013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BKL6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024158">
        <property type="entry name" value="Mt_import_TIM15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007853">
        <property type="entry name" value="Znf_DNL-typ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20922">
        <property type="entry name" value="DNL-TYPE ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20922:SF13">
        <property type="entry name" value="DNL-TYPE ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05180">
        <property type="entry name" value="zf-DNL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51501">
        <property type="entry name" value="ZF_DNL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DNL-type" evidence="6">
        <location>
            <begin position="105"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="70"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7725013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="196" mass="21702" checksum="A6813873CA724DAC" modified="2024-05-29" version="1">MAAWRMQRRLFSLITSNSSRRQPNISLRNDSAPHFLPSISSFLHGEQFNKSQTRTLSEISNPTVETAIEHEAPSSSSVENSVTTDHPAVNNSGVTYSAKSELKISPRHDLAMMFTCKVCETRSVKTISRESYEKGAVVARCGGCNNYHLIADHLGIFGEKCSIEDILAARGEEVKRGNSDTLNLTLEDLVAFKTRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BL47</accession>
    <name>A0AAD5BL47_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Mediator of RNA polymerase II transcription subunit 31</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_026064</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the Mediator complex subunit 31 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011990">
        <property type="protein sequence ID" value="KAI7725266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BL47"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1340:FF:000001">
        <property type="entry name" value="Mediator of RNA polymerase II transcription subunit 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1340">
        <property type="entry name" value="Mediator of RNA polymerase II, submodule Med31 (Soh1)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038089">
        <property type="entry name" value="Med31_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008831">
        <property type="entry name" value="Mediator_Med31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13186">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05669">
        <property type="entry name" value="Med31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0010">Activator</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 7" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="130"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="132"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="153"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="186"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7725266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="198" mass="22886" checksum="AE1AE4CA4EED7A8B" modified="2024-05-29" version="1">MASGQEIGDSSNNNCSPKKVYKDPDDGRQRFLLELEFVQCLANPTYIHYLAQNRYFEDEAFIGYLKYLQYWQRPEYIKYIMYPHCLYFLELLQNASFRNAMAHPANKELTHRQQFYFWKNYRNNRLKHILPRPLPEPTAAPPSNALPPPLPPTATTMAASSTPVSATPVPSPMQYGVPSGPPLPKNDPRNAIDRRKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BMA8</accession>
    <name>A0AAD5BMA8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_021017</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012152">
        <property type="protein sequence ID" value="KAI7724819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BMA8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11446">
        <property type="entry name" value="bHLH_AtILR3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000104">
        <property type="entry name" value="Transcription factor bHLH34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044818">
        <property type="entry name" value="ILR3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46133">
        <property type="entry name" value="BHLH TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46133:SF1">
        <property type="entry name" value="TRANSCRIPTION FACTOR ILR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="8">
        <location>
            <begin position="84"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="51"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="125"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7724819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="246" mass="27016" checksum="89E877ED5C320DE3" modified="2024-05-29" version="1">MISPENNTNWIYDYDGFIQPDDDANFTLSASSGFTCPLQSTCTLNTLNASSSNHPSADLDGSIGDSDGHNDSRSKKRGRSESCSGTSSKACREKLRRDKLNDKFVELASILDPGSLLKNDKAAILVDAVRMVTRLRNEAQKLKDSSSELQEKIKELKAEKNELRDEKQRLKIEKEKLEQQVNSMNPQPTFMAPAYAAAAQGHVGNKLVPVISYPGMGMGMGMAMWQFMPPAAVDTSQDHVLHPPVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BMV1</accession>
    <name>A0AAD5BMV1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_004190</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011709">
        <property type="protein sequence ID" value="KAI7726333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BMV1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041966051" evidence="2">
        <location>
            <begin position="30"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7726333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="100" mass="11059" checksum="04E46A2251CFD74F" modified="2024-05-29" version="1">MKKISTFYTKQFTLLLAVVITHHYYQANSASIDSHLGSVFSGVNRKLRTRAPAPPAPKLNRVTHQTFNVPPPPPPSPPLPLLAPMKHRRKVLTATSIAIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-10-08" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BQ55</accession>
    <name>A0AAD5BQ55_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Tubulin beta chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">M8C21_026182</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">AA19_3_7</strain>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11 12">Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="12">Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the tubulin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011671">
        <property type="protein sequence ID" value="KAI7726428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02187">
        <property type="entry name" value="beta_tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.600:FF:000002">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.20:FF:000002">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000005">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.600">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002453">
        <property type="entry name" value="Beta_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023123">
        <property type="entry name" value="Tubulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01163">
        <property type="entry name" value="BETATUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9 12" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 12" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="14">
        <location>
            <begin position="66"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="15">
        <location>
            <begin position="265"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="444"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="448"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAI7726428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="466" mass="52218" checksum="5C1E6E14B4A74A21" modified="2024-05-29" version="1" fragment="single">KSXXXXXXXXXXXXXXXXXXXXXXXXXGQCGNQIGSKFWEVICTEHTIDHTGRHIGSNPDLELERINVYFNEASGGRYVPRAVLMDLEPGTMDSIRSGPYGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDVVRKEAENCDSLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMLTFSVFPSPKVSDTVVEPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLSNPSFGDLNHLISATMSGVTCSLRFPGQLNSDLRKLAVNLIPFPRLHFFMVGFAPLTSRGSQHYTSLTVPELTQQMWDAKNMMCAADPRHGRYLTASAMFRGKMSTKEVDEQMINVQNKNSSYFVEWIPNNVKSSVCDIPPTGLTMASTFVGNSTSIQEMFRRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVAEYQQYQDATVDEEYDEEGGEEEGEYED</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BQV2</accession>
    <name>A0AAD5BQV2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_032095</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011634">
        <property type="protein sequence ID" value="KAI7726544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BQV2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7726544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="211" mass="23977" checksum="599F0DC96C1A9EB9" modified="2024-05-29" version="1" fragment="single">MEGREYRPCISTKEVKGTISNVASTGTTKYRHYSCLERHALRVFTRKFNISRTEGSCRDWRIAVAVKKSTHNRAEGLKRLGCAAKRAVVFATGFVLAGDGNSTEKKQRKRERGAGDHSEMREKERKGYSGIAELRSLYRDDKELEVEIVISLAKCCVGGESPDCVRLTKQFLLICKNTCYERIISKCNWEVSISKSKETTRTVRNEKKRDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BTR5</accession>
    <name>A0AAD5BTR5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">peroxidase</fullName>
            <ecNumber evidence="3">1.11.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">M8C21_028397</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">AA19_3_7</strain>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 17">
            <text>2 a phenolic donor + H2O2 = 2 a phenolic radical donor + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:56136"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:139520"/>
            <dbReference type="ChEBI" id="CHEBI:139521"/>
            <dbReference type="EC" id="1.11.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="12">Binds 2 calcium ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
        <text evidence="12">Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peroxidase family. Ascorbate peroxidase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.7" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010010986">
        <property type="protein sequence ID" value="KAI7729513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BTR5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140825">
        <property type="term" value="F:lactoperoxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00693">
        <property type="entry name" value="secretory_peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.420.10:FF:000001">
        <property type="entry name" value="Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.420.10">
        <property type="entry name" value="Peroxidase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002016">
        <property type="entry name" value="Haem_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000823">
        <property type="entry name" value="Peroxidase_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019794">
        <property type="entry name" value="Peroxidases_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019793">
        <property type="entry name" value="Peroxidases_heam-ligand_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033905">
        <property type="entry name" value="Secretory_peroxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31388:SF126">
        <property type="entry name" value="PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31388">
        <property type="entry name" value="PEROXIDASE 72-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00141">
        <property type="entry name" value="peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00458">
        <property type="entry name" value="PEROXIDASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00461">
        <property type="entry name" value="PLPEROXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00435">
        <property type="entry name" value="PEROXIDASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00436">
        <property type="entry name" value="PEROXIDASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50873">
        <property type="entry name" value="PEROXIDASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0106">Calcium</keyword>
    <keyword evidence="10 13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0349">Heme</keyword>
    <keyword evidence="9 12" id="KW-0408">Iron</keyword>
    <keyword evidence="6 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Plant heme peroxidase family profile" evidence="14">
        <location>
            <begin position="14"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="12">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="113"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="15">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="15">
        <location>
            <position position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAI7729513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="227" mass="24854" checksum="92288DA1C5A7A888" modified="2024-05-29" version="1" fragment="single">SGLSSSYYDRVCPEALPTIKRVVEDALAQERRMGASLLRLHFHDCFVNLGGPSWKVKLGRRDSNTASRAAANANIPTPSMGLPALIKNFENQGLDEEDLVVLSGAHTLGFAQCRTFRPRIYSATNIDPAFASHLRTICPQVGGDSTLAPLDPTPSSFDVKYFTNLVSKNGLLNSDQALFSGGETAELVSKYSGNHEEFWEDFAESMIKMGDIKPLTGNRGEIRNNCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BUB7</accession>
    <name>A0AAD5BUB7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glutathione S-transferase</fullName>
            <ecNumber evidence="3">2.5.1.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_030503</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Is involved in the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the GST superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010010959">
        <property type="protein sequence ID" value="KAI7729620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BUB7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03185">
        <property type="entry name" value="GST_C_Tau"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045074">
        <property type="entry name" value="GST_C_Tau"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045073">
        <property type="entry name" value="Omega/Tau-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11260:SF710">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11260">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE, GST, SUPERFAMILY, GST DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01152">
        <property type="entry name" value="Main.3:_Omega-_and_Tau-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="4">
        <location>
            <begin position="5"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="5">
        <location>
            <begin position="91"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00047960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU369102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7729620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="231" mass="26892" checksum="83605EF89234A3DA" modified="2024-05-29" version="1">MTQKCEMKLLGSTGSVFVTRVKFVLKLKSIDYEYIEEDLCNKSELLLKSNPTFQQVPVLFHATEQPMIESLAIIEYLDEIQPNVHPLLPSNPSERVQCRILAYTFDTLFYRWIKEFVVTQDKERREELKKLIIRGSVMLEEAFVKLSKGKPFFGGDDIGYLDIVVGAFLGWLKLCERIFGLYVIEEERTPRLTQWGKSMWSREVTRSIIPSHEIHMEFRNMLKKLACASST</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BUJ2</accession>
    <name>A0AAD5BUJ2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tropinone reductase-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_000040</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011159">
        <property type="protein sequence ID" value="KAI7728723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BUJ2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045000">
        <property type="entry name" value="TR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42898:SF6">
        <property type="entry name" value="NADP-DEPENDENT MANNITOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42898">
        <property type="entry name" value="TROPINONE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7728723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="182" mass="19370" checksum="D7E4552BBCEECBDC" modified="2024-05-29" version="1" fragment="single">IKMTTTSAAETSTITKSLRWSLAGTTALVTGGTRGIGFAVVQELAELGASVHTCSRHESELNQRLQEWSDKGLHVTGSVCDASSPTQRQELIDKVSSVFNGKLNILVNNVGTNIRKPTTEYTAEEYSMLMATNLESCYHLCQLAHPLLKASGVGSIVFISSVAGLVHLNSGSIYGATKVSVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BUW3</accession>
    <name>A0AAD5BUW3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CRAL-TRIO domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_020939</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010930">
        <property type="protein sequence ID" value="KAI7729734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BUW3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008526">
        <property type="term" value="F:phosphatidylinositol transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052578">
        <property type="entry name" value="PI_Transfer_CRAL-TRIO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45824">
        <property type="entry name" value="GH16843P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45824:SF22">
        <property type="entry name" value="SEC14P-LIKE PHOSPHATIDYLINOSITOL TRANSFER FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="1">
        <location>
            <begin position="1"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7729734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="167" mass="19436" checksum="093FCA8EAFC7B0BC" modified="2024-05-29" version="1">MQNTTGMENQIRQLVYLVENAILNLPEGQEEMVWLIDFTGWSFSTSVPVKTARETISVLQNHYPQRLAVAFLYSPPRLFEALWKMIKYCLDPKTFQKIKFVYPKNKESVEIMQSCFDADNLPTEFGGKATMKYDHEEFSKLMVQDDLKTAKFWGFDQKIDGNAEKES</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BUW7</accession>
    <name>A0AAD5BUW7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Short-chain dehydrogenase TIC 32, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_019075</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011166">
        <property type="protein sequence ID" value="KAI7728704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BUW7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05327">
        <property type="entry name" value="retinol-DH_like_SDR_c_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055280">
        <property type="entry name" value="TIC32"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48476">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE TIC 32, CHLOROPLASTIC-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48476:SF1">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE TIC 32, CHLOROPLASTIC-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7728704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="324" mass="35525" checksum="FE610546CE731408" modified="2024-05-29" version="1">MGIIRLITGWPGPSGFGSATTAEQVTEGIDASHLTVVITGGASGIGLETARVLALRGAHVIIAARNMEAANDAKQNIIKNNQNAKVDILQLDLSCLKSVKAFADNFLALNIPLNILINNAGIMFCPYQLSQDGVEMQFATNHLGHFYLTKLLLDKMKSTASATGIEGRIVNVSSVAHLHTYKEGIKFDTINDKDSYSEKLAYGQSKLANLLHANELSRLLKEEGANVTVNSVHPGLIMTNLMKHSIKLMRIMNLLTYVLWKNVPQGAATTCYVAVHPNMKGVSGNYYLDCNEWPASDFARDPKMAKKLWDYSNHLLQSVIQHNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BVY4</accession>
    <name>A0AAD5BVY4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_003999</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010704">
        <property type="protein sequence ID" value="KAI7730606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BVY4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034098">
        <property type="term" value="C:VCP-NPL4-UFD1 AAA ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031593">
        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097352">
        <property type="term" value="P:autophagosome maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051228">
        <property type="term" value="P:mitotic spindle disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030970">
        <property type="term" value="P:retrograde protein transport, ER to cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.20.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF200">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 48 HOMOLOG E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATPase AAA-type core" evidence="1">
        <location>
            <begin position="76"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="AAA ATPase AAA+ lid" evidence="2">
        <location>
            <begin position="142"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7730606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="181" mass="20269" checksum="3F0920EB66CDFB11" modified="2024-05-29" version="1">MDVIHLEDESINVEILNSMAVTNKHFSTALDTSNPSALRETEEIGGVEHVTRELQETVQYPVEQPDKFGMSPFKGVLFYGPFGYGKTLLLKAIANECQANFIIIKGLELLAMWFAEGEANVHEDSRHKIFKAAMRKSPISKDADLRALAQYTQGFSGADITEICQRACKYAIRENIEKDIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BW53</accession>
    <name>A0AAD5BW53_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Probable quinone oxidoreductase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_011453</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010751">
        <property type="protein sequence ID" value="KAI7730537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BW53"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035925">
        <property type="term" value="F:mRNA 3'-UTR AU-rich region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070402">
        <property type="term" value="F:NADPH binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003960">
        <property type="term" value="F:quinone reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05286">
        <property type="entry name" value="QOR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000053">
        <property type="entry name" value="Quinone oxidoreductase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047618">
        <property type="entry name" value="QOR-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48106:SF20">
        <property type="entry name" value="2-HALOACRYLATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48106">
        <property type="entry name" value="QUINONE OXIDOREDUCTASE PIG3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="4">
        <location>
            <begin position="11"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00070796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7730537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="413" mass="44346" checksum="B999878E10CBE195" modified="2024-05-29" version="1">MVKAIRVYEYGGPEVLKWEDVELGEPKSGEIKVKIKAAGINYLDIYLRKGIVGDNTPAVPYTPGLECAGVVIAVGPEVTSCKVGDMVAYASYPVCTYAEEQILPADRVVPIPPSIDPIVAAAVIFKGLTTHGLVRKCFKVGPGHTILYHAAAGGVGSLACQWANALGATVIGTVSTKEKAVQAKEDGCHHVIIYKEENFVDRVMEITSGIGVDVVYDSIGKDTFEGSIACLKLHGTLAVFGMASGEHEPLRFQKVAEKGIYYTAPSVLLYTKKPSDLLEASKELFSNIEKGVIRVRVNHKYPLSQAAQAHSDMESQKTKEAVAVVQMDGLAVRVTTKRWAGMGEATRQVSVTVGGNGWAGMADSGGGIRCKRWPRTISVASKTRNGCIDIDFLTVGSLRICDSLDTKHMNMTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BZH7</accession>
    <name>A0AAD5BZH7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">HMA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_026907</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the HIPP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010450">
        <property type="protein sequence ID" value="KAI7731483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BZH7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009626">
        <property type="term" value="P:plant-type hypersensitive response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044577">
        <property type="entry name" value="HIPP4/7/8/17/18/19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46195:SF10">
        <property type="entry name" value="HEAVY METAL-ASSOCIATED DOMAIN CONTAINING PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46195">
        <property type="entry name" value="HEAVY METAL-ASSOCIATED ISOPRENYLATED PLANT PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0488">Methylation</keyword>
    <keyword evidence="4" id="KW-0636">Prenylation</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMA" evidence="7">
        <location>
            <begin position="24"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="7">
        <location>
            <begin position="126"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="67"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="193"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="67"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="98"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7731483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="261" mass="30095" checksum="18D63A8625A8836F" modified="2024-05-29" version="1">MGEEEKKDEKPKEEEEKKKTEDIDEEVVLKVDMHCGACARKVARSLRGFQGVKEVMTDCKDSKVVIKGKELDPTKLSERVQKKTGRKVEIKSPLPKPPEPKPEPEPEKKQQPEPPKEEKKDEPPPVITVVLKMSMHCDACAQALQKRIRKIRGVESVKTELANEQVVVTGVLEPTKLMNDVIKKTRKQASIVVKEEKKEEKKEEEKKEEEKKEEEAPEAKKEDNTNIEAKKMEYWPPKYYMDYSYAPQMFSDENPNACMVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BZL9</accession>
    <name>A0AAD5BZL9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_006108</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010280">
        <property type="protein sequence ID" value="KAI7732225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BZL9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7732225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="47" mass="5411" checksum="B706FF584C274AAE" modified="2024-05-29" version="1" fragment="single">ARLSHRRLRIVRIETTSDNQRIVGLLVPNAVVESVLQDLSWVQEIDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C019</accession>
    <name>A0AAD5C019_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_015349</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010124">
        <property type="protein sequence ID" value="KAI7732871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C019"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="80"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7732871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="114" mass="11553" checksum="CBED15FE8891D801" modified="2024-05-29" version="1" fragment="single">RHPSYILSLLKMASTLKGGGLALSLIILIVASSTFDTISLADARTHARQLANRCEDPGQSDLRGCGGGGGGKGKGGSGGRSPRPGISHGPSPGSSHCKKGCCGMSKKQLCECCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C0B7</accession>
    <name>A0AAD5C0B7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transmembrane protein 45B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_001900</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM45 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010175">
        <property type="protein sequence ID" value="KAI7732722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C0B7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006904">
        <property type="entry name" value="DUF716"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47119">
        <property type="entry name" value="PLANT VIRAL-RESPONSE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47119:SF1">
        <property type="entry name" value="PLANT VIRAL-RESPONSE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04819">
        <property type="entry name" value="DUF716"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="51"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="93"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="123"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="154"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="181"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="236"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7732722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="280" mass="31009" checksum="1850D800279CD553" modified="2024-05-29" version="1">MGSFAGHVVPGTLFLVVGVWHIWCSVVRYASDPNSFRVRVWNPVPGFNGKLKYLELYVISVGAFIDMCIELLYSTHLKFFVNGVLNPHHMNDFEHGGMLLMFFVFSIVALLSEKTSFLPLPEGALCLISAAAFCAEYLLFYFHSTTHQGLEGHYHLLLVLLVALCIISIVAGALMPTSFPADLCSGIAITLQGLWFYQTAFTLYGPSMPAGCKLADNEISCSSHDHEVHGQLLANVQMFGLVFLVLLGVVGSYIFADNRSRKVEFGVVHMKPLDVFHEQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C0R2</accession>
    <name>A0AAD5C0R2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_015353</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010124">
        <property type="protein sequence ID" value="KAI7732875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C0R2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042221481" evidence="2">
        <location>
            <begin position="32"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7732875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="97" mass="9644" checksum="3AC9C02D639306BB" modified="2024-05-29" version="1">MASVVKQGLALALIMLIVITSSFENISVVDARSPYIRARHLATDCNSPKQGDVAGCGGGGGSGGSSRPGGPSGPAPGSSRCRKGCCGTNKMGKCICC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C1A7</accession>
    <name>A0AAD5C1A7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_033700</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010038">
        <property type="protein sequence ID" value="KAI7733282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C1A7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000095">
        <property type="entry name" value="protein STRUBBELIG-RECEPTOR FAMILY 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000125">
        <property type="entry name" value="Protein STRUBBELIG-RECEPTOR FAMILY 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47985">
        <property type="entry name" value="OS07G0668900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47985:SF44">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE PBS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="71"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7733282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="360" mass="39557" checksum="809730144980DBB4" modified="2024-05-29" version="1" fragment="single">MKHDQASSMVHTKSFETTDTISLKPPPFDHQKSFDDDGFSAIPIVPKKVHVAPPDVTSYSVADLQLATNSFSADNLVGEGSTGRVFRAQFEDGKVVAVKKILPSVLPGANSDDFVDMVSSISRLLHPNITELIGYCSEHGQHLLVYEFLKNGSLYDFLHLSDEYSKPLIWNTRVKIALGTARALEYLHEVCSPSVIHKNIKSANILLDSELNPHLSECGLASLISDADQNSGYSAPEVSMSGQYTMKSDVYSFGVTMLELLTGRKPFDSSRTRAEQSLVRWATPQLHDMDALAKMVDPALKGLYPVKSLSRFADVIALCVQPEPEFRPAMSEVVEALVRLVQRANMSKRTVGVEQGRSGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C2A3</accession>
    <name>A0AAD5C2A3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GDSL esterase/lipase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_021690</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009908">
        <property type="protein sequence ID" value="KAI7733772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C2A3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01837">
        <property type="entry name" value="SGNH_plant_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051058">
        <property type="entry name" value="GDSL_Est/Lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035669">
        <property type="entry name" value="SGNH_plant_lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45648:SF1">
        <property type="entry name" value="GDSL ESTERASE_LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45648">
        <property type="entry name" value="GDSL LIPASE/ACYLHYDROLASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_4G14700)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7733772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="356" mass="38780" checksum="CA8166C7658C48EE" modified="2024-05-29" version="1" fragment="single">LVKTVAADGGGTLGASYIFGDSLVDAGNNNFLPTLSKANIKPNGIDFKQSGGNPTGRYTNGRTIGDIIGEELGQQHYAVPFLDPNSTGEVILYGVNYASGGGGIMNATGRIFVNRLSMDIQVDYFNITRKQIDKLLGASKAREHVMKKSIFSVSIGSNDFLNNYLLPVLSIGARVSQTPDGFVDDLISHLRSQLTRLYQLDARKFILGNVGPIGCIPYQRTINQLNENECVALPNELALRYNAKLKDLVAQLNDNLPGATFVHANVYDLVLELITNYDKYGFKTASKACCGNGGQYAGIIPCGPTSTLCEDRDKHVFWDPYHPSEAANVLIAKQLLQGDPKYVSPMNLKQLRDLNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C2H3</accession>
    <name>A0AAD5C2H3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Serine carboxypeptidase-like 18</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_013933</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009890">
        <property type="protein sequence ID" value="KAI7733877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C2H3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019748">
        <property type="term" value="P:secondary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF224">
        <property type="entry name" value="SERINE CARBOXYPEPTIDASE-LIKE 7 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7733877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="358" mass="41250" checksum="53D0F90F9DB3293C" modified="2024-05-29" version="1" fragment="single">PLTLNFEISTIEKPILKIVHDSLTKVASIIFLDQPAGSGFSYGKTQEAYIMNDTLSSIHAYDFMKKWLVDHPKFLNNPLYLGGDSYSGILVPMIVQEIYNDRSGDYNSQIEFAHNMALLSDAIYESTKEHCHGDYFNVDPNNFLCIHDLQVVDKCLERIKMAQILEPNCLISNPLTSDPFRSDLPQVQTRLCREDNYIYTYAWANRRDVREALHIHEEFGDIEWVQCNTSLKFSFSKETISYTHNVQSSVGYHRRLTKRKCRALIYSGDHDMAVPYLSTLNWIQSLNMSLVDAWRPWFVNEQVAGYTKKYSKGDYNLTFATVKGGGHTAPEYKHKECLSMLKMWIANGTINDVYKETN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C405</accession>
    <name>A0AAD5C405_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">HTH myb-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_010362</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009616">
        <property type="protein sequence ID" value="KAI7734926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C405"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000002">
        <property type="entry name" value="Myb family transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044787">
        <property type="entry name" value="HHO5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006447">
        <property type="entry name" value="Myb_dom_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01557">
        <property type="entry name" value="myb_SHAQKYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31003:SF30">
        <property type="entry name" value="MYB DOMAIN, PLANT, HOMEODOMAIN-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31003">
        <property type="entry name" value="MYB FAMILY TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="6">
        <location>
            <begin position="139"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="121"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="231"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="122"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="231"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7734926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="275" mass="30184" checksum="9F825EE723FCFC06" modified="2024-05-29" version="1">MVLKEEAMVFKKSSRVEPVFEEFIPLKKSCDEEVEEDRDVEKDDLTVDGSHQPFPGRAFIPLKGCYGFTMVGTPGEKDGEELPIPGLSLVTPGIGIKNSMRGNNLVNKTSPNTKLTPCFAPNDQSNGVVTRGQSPQQHSSRKQRRCWSTELHRRFVNALQQLGGSQAATPKQIRELMQVEGLTNDEVKSHLQKYRLHTRRLPSSNASSANQTVGVLGGGMWTPPQDQYIESSKHGISQSSSPDGPFLTGTTTTTGGDSMDDGEDAKSENYCWKGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C5B6</accession>
    <name>A0AAD5C5B6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_019803</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009594">
        <property type="protein sequence ID" value="KAI7735005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C5B6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050648">
        <property type="entry name" value="F-box_LRR-repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13382:SF16">
        <property type="entry name" value="F-BOX PROTEIN SKIP28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13382">
        <property type="entry name" value="MITOCHONDRIAL ATP SYNTHASE COUPLING FACTOR B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="2" id="KW-0934">Plastid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0793">Thylakoid</keyword>
    <feature type="domain" description="F-box" evidence="5">
        <location>
            <begin position="26"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF12937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7735005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="290" mass="32604" checksum="EA6FD37C4138C64F" modified="2024-05-29" version="1">MNMESSHETTHKQHQMNQDSSNMEQPHESLYLVLPYLPLFELLAMAQTCRSFNDALNSDILPWLNIVVYDKVRRSRLSDEVLIKITSKAMGRLRSLILVNCGKVTDDGLLNVVANNPGINKLHVTQCTGLTAEGIIKAVTTLNQHNTTLKTLKINGISNITKEHLQTLHSLLNSDDSSSIDVEICPKCDEPRMVYGCPSETCNRKRTTGSCRGCKFCVLRCEECGICIEFDEEENEVACEDMLCEGCWISLPKCDFCNKPYCNKHAYKQCVVPGSFGFVCEACYMKIDEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C878</accession>
    <name>A0AAD5C878_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">1,3-beta-glucan synthase</fullName>
            <ecNumber evidence="3">2.4.1.34</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">1,3-beta-glucan synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">M8C21_012630</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">AA19_3_7</strain>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>[(1-&gt;3)-beta-D-glucosyl](n) + UDP-alpha-D-glucose = [(1-&gt;3)-beta-D-glucosyl](n+1) + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21476"/>
            <dbReference type="Rhea" id="RHEA-COMP:11146"/>
            <dbReference type="Rhea" id="RHEA-COMP:14303"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37671"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.4.1.34"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 48 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.34" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010009194">
        <property type="protein sequence ID" value="KAI7736755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C878"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000148">
        <property type="term" value="C:1,3-beta-D-glucan synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003843">
        <property type="term" value="F:1,3-beta-D-glucan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006075">
        <property type="term" value="P:(1-&gt;3)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058851">
        <property type="entry name" value="CALS1_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026899">
        <property type="entry name" value="FKS1-like_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003440">
        <property type="entry name" value="Glyco_trans_48_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12741:SF48">
        <property type="entry name" value="1,3-BETA-GLUCAN SYNTHASE COMPONENT FKS1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12741">
        <property type="entry name" value="LYST-INTERACTING PROTEIN LIP5 DOPAMINE RESPONSIVE PROTEIN DRG-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25968">
        <property type="entry name" value="CALS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14288">
        <property type="entry name" value="FKS1_dom1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02364">
        <property type="entry name" value="Glucan_synthase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM01205">
        <property type="entry name" value="FKS1_dom1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0133">Cell shape</keyword>
    <keyword evidence="11" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="111"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="148"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="182"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="227"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="288"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="340"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="363"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="1,3-beta-glucan synthase component FKS1-like" evidence="15">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="1072"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM01205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAI7736755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="1072" mass="123967" checksum="986EF1AE4CDFB06E" modified="2024-05-29" version="1" fragment="single">YGGEEEAFLTKVVTPIYKVIAREAAWSKVGRSKHSQWRNYDDLNEYFWSVDCFRLGWPMRADADFFSYPDKSRESEKNGDGKPSGIHRRVDKVNFVEIRSYWHVFRSFDRMWSFFILCLQAMIIVAWNGDGNPASIFDSDVFKKVLSVFITASILKLGQAVLDVVLNWKARQCMPFYVKLRYLLKVISAAAWVIILPVTYAYTSKDQAGLAQTIKGWFGNSQGSPSLFILAVVIYLSPNMLAAVLFLFPFIRRYLESSDYRVMMLMMWWSQPRLYVGRGMHESTFSLFKYTTFWILLIITKLAFSYYLEIKPLVGPTKAIMSVHINTYAWHEFFPQAKNNIGVVVALWAPIILVYFMDTQIWYAIFSTLFGGVYGAFRRLGEIRTLGMLRSRFESLPGAFNGCLIPPEKSDPVKKKGLKATLSRNYDAIAFHKGKEAARFAQLWNKIITSFREEDLINNREMNLLLVPYWADRDLDLIQWPPFLLASKIPIALDMAKDSNGKDRELKKRIENDNYMSCAVRECYASFRNIIKFLVRGSREKKVINDIFAEVDKHIESGDIVSDFKMSALPILYDHVVKLIKYLLTNKQEDRDQVVILFQDMHEVVTRDIMEERLDWQGDEVDEQIQLFQPAGAILFPTPESEAWKEKINRLYLLLTVKESAMDVPSNLEARRRISFFSNSLFMDMPSAPKVRNMISFSVLTPYYTEEVLFSLHDLEVPNEDGVSILFYLQKIFPDEWNNFLERMNCSSEDDLKGDDELEDQLRLWASYRGQTLTKTVRGMMYYRKALELQAFLDMAKDEDLMEGYKAIELNEDQMKGERSLWAQCQAVADMKFTYVVSCQQYGVQKRSGDPRAQNVLRLMTEYPSLRVAYIDEVEEPSKDATKKINHKVYYSALVKAMPNSNASETGQNLDQDNYMEEALKMRNLLQEFLKKHDDVRYPTILGLREHIFTGSVSSLAWFMSNQETSFVTIGQRLLANPLKVRFHYGHPDVFDRLFHLTRGGVSKASKIINLSEDIFAGFNSTLREGNVTHHEYIQVGKGRDVGLNQISLFEAKIANGNGEQTLSRDLYRLGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C9J0</accession>
    <name>A0AAD5C9J0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Hcy-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_028757</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008948">
        <property type="protein sequence ID" value="KAI7737821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C9J0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008898">
        <property type="term" value="F:S-adenosylmethionine-homocysteine S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033528">
        <property type="term" value="P:S-methylmethionine cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.330">
        <property type="entry name" value="Homocysteine-binding-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017226">
        <property type="entry name" value="BHMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003726">
        <property type="entry name" value="HCY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036589">
        <property type="entry name" value="HCY_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051486">
        <property type="entry name" value="Hcy_S-methyltransferase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007020">
        <property type="entry name" value="PRK09485.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46015:SF9">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46015">
        <property type="entry name" value="ZGC:172121"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02574">
        <property type="entry name" value="S-methyl_trans"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037505">
        <property type="entry name" value="Betaine_HMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82282">
        <property type="entry name" value="Homocysteine S-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50970">
        <property type="entry name" value="HCY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Hcy-binding" evidence="6">
        <location>
            <begin position="11"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7737821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="317" mass="34433" checksum="4AA08849A7E18B87" modified="2024-05-29" version="1">MGVNNGTVQTLMSDFLTQSGGVAVIDGGFATELERHGADLNDPLWSAKCLLTSTDLVRQVHLDYLEAGADIIITASYQATIQGFGAKGFSKEESEAMLKRSVDIACEARKIYYERCCNSASNFSSDGKILKNRPILIAASVGSYGAYLADGSEYSGDYGDAMDLEFLKSFHRRRVQILAESGADLIAFETVPNKLEAQAYAELLEEGIRIPAWFSFNSKDGVNVVSGDSLTECAKIADSCHKVTTKPILIYPNSGETYDADLKQWVQNTGVPDEDFVSYVNKWCEIGASLVGGCCRTTPNTIKAICRTLPSRSSYLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CAI4</accession>
    <name>A0AAD5CAI4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AMP-dependent synthetase/ligase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_032144</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008927">
        <property type="protein sequence ID" value="KAI7737880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CAI4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272:SF87">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="1">
        <location>
            <begin position="23"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7737880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="302" mass="33719" checksum="D41147F945068B08" modified="2024-05-29" version="1">MKRGYRHEEAAPRFDKIIFNKVKEGLGGKLRLILSGAAPLSASVETFLRVVTCAHVLQGYGLTESCAGSFVAQPNELSMIGTVGPPLPNVDVCLMSVPEMGYDALAATTPRGEVLLRGPSLFSGYYKRQDLTKEVLLDGWFHTGDIGEWQPDGTMKIIDRKKNIFKLSQGEYVSVENLETIFSLVPVLDAIWIYGNSFESFLVAVVNPNKELLEKWAAENGVAGEFCTICENSKTNAYILGELTSIAKEKKLKGFEFVKAVHLDPLPFDMDRDLITPTFKKKRAQFLKYYQNVVDGMYKSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CBK6</accession>
    <name>A0AAD5CBK6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Acyl-CoA dehydrogenase family member 11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="26" type="ORF">M8C21_002849</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="26">AA19_3_7</strain>
            <tissue evidence="26">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Acyl-CoA dehydrogenase, that exhibits maximal activity towards saturated C22-CoA. Probably participates in beta-oxydation and energy production but could also play a role in the metabolism of specific fatty acids to control fatty acids composition of cellular lipids in brain.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a 2,3-saturated acyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = a (2E)-enoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:44704"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:58856"/>
            <dbReference type="ChEBI" id="CHEBI:65111"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:44705"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>docosanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-docosenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:47228"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:65059"/>
            <dbReference type="ChEBI" id="CHEBI:74692"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:47229"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>eicosanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-eicosenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:47236"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57380"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:74691"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:47237"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>hexacosanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-hexacosenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:48216"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:64868"/>
            <dbReference type="ChEBI" id="CHEBI:74281"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:48217"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>tetracosanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-tetracosenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:47232"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:65052"/>
            <dbReference type="ChEBI" id="CHEBI:74693"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:47233"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>tricosanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-tricosenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:48220"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:90118"/>
            <dbReference type="ChEBI" id="CHEBI:90119"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:48221"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Lipid metabolism; fatty acid beta-oxidation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="26">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009024">
        <property type="protein sequence ID" value="KAI7737451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CBK6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003995">
        <property type="term" value="F:acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033539">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05154">
        <property type="entry name" value="ACAD10_11_N-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.110.10:FF:000002">
        <property type="entry name" value="Acyl-CoA dehydrogenase fadE12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.540.10:FF:000016">
        <property type="entry name" value="acyl-CoA dehydrogenase family member 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1200.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041726">
        <property type="entry name" value="ACAD10_11_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050741">
        <property type="entry name" value="Acyl-CoA_dehydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002575">
        <property type="entry name" value="Aminoglycoside_PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48083:SF13">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE FAMILY MEMBER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48083">
        <property type="entry name" value="MEDIUM-CHAIN SPECIFIC ACYL-COA DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01636">
        <property type="entry name" value="APH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0274">FAD</keyword>
    <keyword evidence="9" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoglycoside phosphotransferase" evidence="23">
        <location>
            <begin position="43"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="25">
        <location>
            <begin position="452"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="24">
        <location>
            <begin position="525"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="22">
        <location>
            <begin position="638"/>
            <end position="786"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00040622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00046026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00049140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF01636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="EMBL" id="KAI7737451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="794" mass="88045" checksum="460FC3718DAF81CF" modified="2024-05-29" version="1">MGQRTGELVGEVNSAHKFDTDALFRYCSVHVEGFPVSSSRFSVSQFGHGQSNPTYLLELQSDHLVKRYVMRKKPPGKLLQSAHAVEREFQVLHALGTHTLVPVPKVFCLCTDSSVIGTPFYIMEFLEGRIFVDPMLPGVAPDRRRALYHATANALASLHSADVDAIGLGGYGRRNNYCNRQVERWAKQYIASTGEGKSERNPKMLMLIDWLRQNIPSEDSSGSTAGLVHGDFRMDNLVFHPIEGKVRHNNGFELDITEGVPSLEEYLSDYCSAAGKQWPVASWKFYIAFSLFRGASILAGVHSRYIMGNASGGKRAQDAGEKANDLIQIAWSYIQRETVLSQNPPSVTRGKDYAYGIANDKKDEGLESGGRFVPNKKVKELRDKLIKFMEERVYPMEQEFSKLAQSSMRWTVHPQEEKLKEIAKKEGLWNLFIPLDSAARAREILFEGRSDDAIGTGFPNQLGAGLSNLEYGYLCEIMGRSVWAPQVFNCGAPDTGNMEVLLRYGNKEQLQEWLIPLLSGTIRSGFAMTEPQVASSDATNIECSIRREGDSYVINGKKWWTSGAMDPRCKLLILMGKTDFNAPIHKQQSMILVDINTPGVKILRPLTVFGFDDAPHGHAEVSFENVRVPAKNILLGEGRGFEIAQGRLGPGRLHHCMRLIGAAERGIQLMVQRSLQRKTFGKLIAQHGSFVSDLAKCRIELEKTRLLVFEAADQLDRFGNKKARGALAMAKVAAPNMAITVLDTAMQVHGAAGVSGDTVLAHLWATARTLRIADGPDEVHLGTIAKLELQRAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CDL3</accession>
    <name>A0AAD5CDL3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Germin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_020284</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Secreted</location>
            <location evidence="1 10">Extracellular space</location>
            <location evidence="1 10">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the germin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008758">
        <property type="protein sequence ID" value="KAI7738664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CDL3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02241">
        <property type="entry name" value="cupin_OxOx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006045">
        <property type="entry name" value="Cupin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001929">
        <property type="entry name" value="Germin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019780">
        <property type="entry name" value="Germin_Mn-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31238">
        <property type="entry name" value="GERMIN-LIKE PROTEIN SUBFAMILY 3 MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00190">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00325">
        <property type="entry name" value="GERMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00835">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00725">
        <property type="entry name" value="GERMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0052">Apoplast</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 8 10" id="KW-0464">Manganese</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 8 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0964">Secreted</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="142"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="Cupin type-1" evidence="12">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU366015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7738664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="162" mass="18171" checksum="15BA8A3B6417CACB" modified="2024-05-29" version="1">MARIDYAPGGLNPPHTHPRATEIVFVLEGELDVGFMTIENKLFSKTIMKGEIFTFPRGLIHFQINNGNVSATIIAAFNNQFPGTQRVVTAMFASSPTIEDVVLTKTFQVGTKEEKKVKSRLAPKNMSSSPSSFSSTIRPMRYSSLIIHYFFIFLNTLYVINF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CEU0</accession>
    <name>A0AAD5CEU0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_020823</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008472">
        <property type="protein sequence ID" value="KAI7740287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CEU0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.790">
        <property type="entry name" value="Surp module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000061">
        <property type="entry name" value="Surp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035967">
        <property type="entry name" value="SWAP/Surp_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052650">
        <property type="entry name" value="Zinc_finger_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36886:SF7">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36886">
        <property type="entry name" value="PROTEIN FRIGIDA-ESSENTIAL 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01805">
        <property type="entry name" value="Surp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00648">
        <property type="entry name" value="SWAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109905">
        <property type="entry name" value="Surp module (SWAP domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50128">
        <property type="entry name" value="SURP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SURP motif" evidence="5">
        <location>
            <begin position="162"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="4">
        <location>
            <begin position="525"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="2">
        <location>
            <begin position="525"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="16"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="232"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="396"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="546"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="811"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="44"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="61"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="90"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="114"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="396"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="470"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="489"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="563"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="835"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="995"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7740287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="995" mass="109789" checksum="E9565056A4DE425D" modified="2024-05-29" version="1" fragment="single">GFADEYVVAARNYQQYTTRLPPPPSFQQPGTSSYVHPRPGPGSGTMTSGQSYVSWSQGQRMPPPPLVPPPGHFQTPRIPPPGQHFYRVPSVPPPLIPPPSSSYFTPARSGSFILPPPPPPSSPPPGPPPLPPPSPALNGNVHVNGLSDVPPPAPRDENTVRKIEVLCQYIAKNGEEFEEMTRQKEVGNPEFEFLFGGAAGSEAAISHEYFKWMKSKCRSSGLLEGGNNMDLSSKSSVVGTSREDISHSPAGSDMDMEDDITLPEEKHDAAAISNDVDTKAENIVCESPRAIEQGPNICFNDTQLENTKMKDGVADSKLSLDRNTEQDSVNLYKETNQSEQSPFKLIQGYASDDNSENDNDLHFENLSPVAVTPQVKEHTTLSVPVVDGSLDITSKVSEHSIETEKLENRTDGGASMKQNNNLEGDGTTAPKNGGAQKENNNVKLETDKFGRLVKRGGSDSDSDSDDYSTRRRVKRGRSRSRSHSPYGRRSPRRRRRSPSRSRSPRKRGRSRSPNNRRNRSHVQEVCYDFLRGKCFRGAACRYLHKDKSEESRRHKSKQYNNEVSDRLKDSDKSVPVKDEVSHHVSRSSERFNEELVEPPSHSADVTSGHETEHPATEMPIRGQIPISGHSINQTSQPEPQQANAYNLMPSATSWNTLPPPPPRPQLGRPVPQYQLTQPPFQTNYPFQPFVTPYGPEPTSHVGVYPMNNYPILQQPVGPAVINQENFAQTSSPNLVSSNTREQPTGVNAVQSFLQNPVADNLSDFGRSRIMSHYNPYASTFDQPLSTKFGSGVFNQDYSASTNQTNTLGEGQTHNFAPNPPMSGGDQYDPLFDSVDPSSNSVKKSNTIAASEENDGFGETADAEVGAVENDSPSPSSPIDLPDMTAGDIEIDQVKNKKSKDSRSMKLFKIALADFVKEVLKPSWRQGNMSKEAFKTIVKKTVDKVSGAMKKHQIPKSQTKINHYIDSSQRKLTKLVMGYVDKYVKLKVGNGYEFDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CF63</accession>
    <name>A0AAD5CF63_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA topoisomerase (ATP-hydrolyzing)</fullName>
            <ecNumber evidence="5">5.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_001194</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP-dependent breakage, passage and rejoining of double-stranded DNA.</text>
            <dbReference type="EC" id="5.6.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TOP6A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.2" evidence="5"/>
    <dbReference type="EMBL" id="JAMZMK010008349">
        <property type="protein sequence ID" value="KAI7740761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CF63"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000228">
        <property type="term" value="C:nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003918">
        <property type="term" value="F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042138">
        <property type="term" value="P:meiotic DNA double-strand break formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000706">
        <property type="term" value="P:meiotic DNA double-strand break processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00223">
        <property type="entry name" value="TOPRIM_TopoIIB_SPO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1360.10:FF:000003">
        <property type="entry name" value="DNA topoisomerase 6 subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1360.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013048">
        <property type="entry name" value="Meiotic_Spo11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002815">
        <property type="entry name" value="Spo11/TopoVI_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036078">
        <property type="entry name" value="Spo11/TopoVI_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034136">
        <property type="entry name" value="TOPRIM_Topo6A/Spo11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10848">
        <property type="entry name" value="MEIOTIC RECOMBINATION PROTEIN SPO11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10848:SF3">
        <property type="entry name" value="MEIOTIC RECOMBINATION PROTEIN SPO11-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21180">
        <property type="entry name" value="TOP6A-Spo11_Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01551">
        <property type="entry name" value="SPO11HOMOLOG"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01550">
        <property type="entry name" value="TOP6AFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56726">
        <property type="entry name" value="DNA topoisomerase IV, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="Topoisomerase 6 subunit A/Spo11 TOPRIM" evidence="12">
        <location>
            <begin position="45"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF21180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7740761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="406" mass="47052" checksum="4652FD7A5ACB4129" modified="2024-05-29" version="1" fragment="single">FRLVMGWLRFSEADRIFNCINHPDTAHSIPVLVEEVKDIISVAEYILVVEKESVFQRLANDCFCKNNRCIVITGRGYPDIPTRRFLRLLIEQLHLPTYCLVDCDPYGFDILTTYRFGSMQMAYDAKIMKLPEINWLGVLPSDAEKFNVPQQCLLPMTTEDKIKTEAILNRCYLQREVPQWRLELQLLLQSGVKFEIEALSVHSLTFLSKRYIPSKIQVFTMDPCADMECSLRVHSKCGCCVMKTHDILRAICGVYTVEWDVEKNLFKISGEVDPSILLKAVLSTGEHAELVSVKIKHPQLRHRNYSYGSYGPTNGYHMPYYRDAGYSHRSLANYPYYETNGHYYPYSLPRDTLPIEYPSSYGYNYNHTTTNDYQYPPPRATYVPSYPPQEYDPYANFDTISPCTIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CK11</accession>
    <name>A0AAD5CK11_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_017939</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007760">
        <property type="protein sequence ID" value="KAI7743267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CK11"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd10017">
        <property type="entry name" value="B3_DNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.330.10:FF:000006">
        <property type="entry name" value="B3 domain-containing transcription repressor VAL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.330.10">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003340">
        <property type="entry name" value="B3_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015300">
        <property type="entry name" value="DNA-bd_pseudobarrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057743">
        <property type="entry name" value="Zfn_VAL1-3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011124">
        <property type="entry name" value="Znf_CW"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46245">
        <property type="entry name" value="B3 DOMAIN-CONTAINING PROTEIN OS07G0563300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46245:SF2">
        <property type="entry name" value="B3 DOMAIN-CONTAINING TRANSCRIPTION REPRESSOR VAL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02362">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07496">
        <property type="entry name" value="zf-CW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25813">
        <property type="entry name" value="zf_VAL1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01019">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101936">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50863">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51050">
        <property type="entry name" value="ZF_CW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TF-B3" evidence="9">
        <location>
            <begin position="303"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="domain" description="CW-type" evidence="10">
        <location>
            <begin position="522"/>
            <end position="572"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7743267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="589" mass="65186" checksum="C86317638A63CDC6" modified="2024-05-29" version="1">MMDQSVVCMNILCQTELAAGADCIKGWSLRSGGFANLCHNCGNAYEQQKFCELFHLDDTGWRECTSCGKRLHCGCIASSAFVVLLDNGGVTCVSCSNSPGNYPKTREEKPKETGITVENKVGEARAACIDNHVKTGNLSKIDLTILDGEAADSSEKLLLPSDNHDASKSQTKPHEKAACSSIENAHKDVYGSPVQTNLTIGLNGSPAKAVFNTSNKAVEEQEVKPTLSYFPQGIWSRHILPKSIIPQGFSNANVDVSSQIRVARPPVEGRIKNQLLPRYWPRITEQELQQISGDSNATIVPLFEKVLSASDAGRIGRLVLPKACAEAYFPPISQPEGLPLKINDVKGKEWMFQFRFWPNNNSRMYVLEGVTPCIQSMQLQAGDTVTFSRMEPEGKLLMGFRKASTNASVQGSTYSHLSELPQNASWPANEQHGIKLMAGAFPPSMLAPDKKRSRNIGSKSKRLLIESQDSLELMHTWEELQDMLFPSPLLKATIVTIEDNDLEEYEEPPVFGKGSIFTVCSSGEQEQWAECDNCSKWRRLPADFLLPPRWTCQDNVWDQHRASCLAPDELNPRELENLLRNAKVSALLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CNK0</accession>
    <name>A0AAD5CNK0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">M8C21_034033</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">AA19_3_7</strain>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007371">
        <property type="protein sequence ID" value="KAI7745067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CNK0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015421">
        <property type="term" value="F:ABC-type oligopeptide transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010329">
        <property type="term" value="F:auxin efflux transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010328">
        <property type="term" value="F:auxin influx transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090374">
        <property type="term" value="P:oligopeptide export from mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18577">
        <property type="entry name" value="ABC_6TM_Pgp_ABCB1_D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18578">
        <property type="entry name" value="ABC_6TM_Pgp_ABCB1_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03249">
        <property type="entry name" value="ABC_MTABC3_MDL1_MDL2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000009">
        <property type="entry name" value="ABC transporter B family member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000066">
        <property type="entry name" value="ABC transporter B family member 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000025">
        <property type="entry name" value="ABC transporter B family member 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000044">
        <property type="entry name" value="ABC transporter B family member 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394:SF16">
        <property type="entry name" value="ABC TRANSPORTER B FAMILY MEMBER 4-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394">
        <property type="entry name" value="ATP-DEPENDENT PERMEASE MDL1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="42"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="93"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="270"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="306"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="672"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="716"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="819"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="898"/>
            <end position="923"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="14">
        <location>
            <begin position="46"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="13">
        <location>
            <begin position="368"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="14">
        <location>
            <begin position="676"/>
            <end position="962"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="13">
        <location>
            <begin position="997"/>
            <end position="1234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="614"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="625"/>
            <end position="644"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAI7745067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="1240" mass="134256" checksum="04DDF3CFBDB7CABD" modified="2024-05-29" version="1">MVKIGAQETPKAGSHSTKAQETECVNSVPFYKLFAFADFKDYILMIVGTIGAIGNGICMPMMTILLGDFINAFGENQNNNNVVHVVSKVSLKFVYLAIGTGVGSFMELAMWTVTGERQASRIRNLYLKSILRQDVSFFDKETTTGEIVGRISGDTVLIQDAMGEKVGKFIQLMATFLGGLTIAFIKGWLLTLVLLISVPLMVVSSGVMSGRISKMASRGQIAYAKAANVIEQTLGSIRTVASFVGEKKALAHYNNALTDAYDSGVNEGRAVGLGLGSVVLIMYCSYAMAVWYGAKMVLEKGYTGGTVVNVIMALVTGSTSLGPASPCLSAFASGRAAAFKMFETINRKPEIDSYDTKGKVLKDICGDIELKDVYFTYPARPDEEIFRGFSLCIPAGTTAALVGQSGSGKSTVISLIERFYDPQAGEVLIDGINLKEFQVKWIRQKMGLVSQEPVLFASSIKDNIMYGKDNASMDEIRVAAELANAAKFIDKLPQGMDTMVGEHGTQLSGGQKQRIAIARAILKDPRILLLDEATSALDATSERVVQEALDRIMVNRTTVIVAHRLSTVRNADMIAVLHHGKIVEKGSHSQLLQDPEGAYAQLTKLQHVNNDAISNGSEDQEISMELTTPSSQRTPNEQSPSSSHDSMEENAATRKPPNVPLRRLAYLNKPELPILIMGAIAAITNGAVFPTFGILISKMIKTFYEPPHKMKSDSKFWAIMFILLGVVACISLSARSYLFSVAGCRLIKRMRSLCFEKVINMEIGWFDKLENSSGAIGARLSADAAAVRALVGDALAQLVQDLSSVVTGLTIAFATCWQLAFIILVLIPLVVANGFVYLKFTKGFSTDAKIMYEEASQVASDAVRSIRTVASFCAEEKVMQFYRDKCAGPERMGIKQGLIAGIGFGVSYSLLFCVYAASFYAGARLVKAGTTTFSDVFRVFYALTMAAVAVSHSGKFAPDTSKAKSSVASVFAILDRESEIDPSDESGMMLHHVKGEIEFRHVSFRYPTRPDVEIFHDLCLTINSGKTVALVGESGSGKSTIISLLQRFYNPVSGCITLDGYELQKFQLKWLRLQMGLVSQEPVLFNDTIQANIAYGKDGDATEAQILAASELANAHGFISGLHQGYNTMVGERGVQLSGGQKQRVAIARAIVKSPQILLLDEATSALDAESEKVVQDALDRVIVSRTTVVVAHRLSTIKGADLIVVIKNGVVVEKGKHEDLINIKDGSYKSLVAAIHSTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CNX2</accession>
    <name>A0AAD5CNX2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Aspergillus nuclease S1</fullName>
            <ecNumber evidence="4">3.1.30.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_015306</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage to 5'-phosphomononucleotide and 5'-phosphooligonucleotide end-products.</text>
            <dbReference type="EC" id="3.1.30.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nuclease type I family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.30.1" evidence="4"/>
    <dbReference type="EMBL" id="JAMZMK010007217">
        <property type="protein sequence ID" value="KAI7745518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CNX2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000014">
        <property type="term" value="F:single-stranded DNA endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006308">
        <property type="term" value="P:DNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11010">
        <property type="entry name" value="S1-P1_nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.575.10:FF:000002">
        <property type="entry name" value="Endonuclease 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.575.10">
        <property type="entry name" value="P1 Nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008947">
        <property type="entry name" value="PLipase_C/P1_nuclease_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003154">
        <property type="entry name" value="S1/P1nuclease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33146:SF27">
        <property type="entry name" value="ENDONUCLEASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33146">
        <property type="entry name" value="ENDONUCLEASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02265">
        <property type="entry name" value="S1-P1_nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48537">
        <property type="entry name" value="Phospholipase C/P1 nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042119521" description="Aspergillus nuclease S1" evidence="12">
        <location>
            <begin position="24"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7745518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="285" mass="31921" checksum="BCCDC7AA80ABA29C" modified="2024-05-29" version="1">MESLRYPALLLLVVFIAPVAVRGWGVDGHFITCKIAQGRLGQTAADAVNSLLPEYAGGDLASLCSWADHVKFRYHWSSALHYIDTPDNLCTYQYRRDCKDEDGVTGRCVAGAIMNYTTQLLDYGKQTSEYNLTEALLFLSHFMGDIHQPLHVGFTSDRGGNTIDVHWYTRKAVLHHVWDDSIIETEEERFYGSNVETLTNAIETNITKVWGDQVQAWEKCSGNQKTCPNIYATEGIKAACNWAYKGAANGSVLEDEYFLSRLPIVNWRLAQGGVRLAANLNRIFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CPT5</accession>
    <name>A0AAD5CPT5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_007304</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007093">
        <property type="protein sequence ID" value="KAI7745883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CPT5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7745883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="189" mass="21134" checksum="103E760F8EFA45D0" modified="2024-05-29" version="1" fragment="single">PSCTGALLQIHADDTATTSYCQPQAEIVNQFLEFQTQDVMMQPQQGSLLNMILNFSNPQPSFENPFELHASNPKPNPILKSNPVPEPQSNIESEFEPNFETEAECVIVAESPFEDPELPLALDSIKGRPQPVQDLVKELTNLVGHSYAVRVVSPNGMYPEPVDEAVAYSELLQIFLNKWLNVTLLHWFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CQG1</accession>
    <name>A0AAD5CQG1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_001245</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 4">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PPP phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="4"/>
    <dbReference type="EMBL" id="JAMZMK010007474">
        <property type="protein sequence ID" value="KAI7744636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CQG1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07415">
        <property type="entry name" value="MPP_PP2A_PP4_PP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047129">
        <property type="entry name" value="PPA2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45619">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE PP2A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0464">Manganese</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="5">
        <location>
            <begin position="142"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7744636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="285" mass="32667" checksum="017446E288C3E200" modified="2024-05-29" version="1" fragment="single">GTKDTKIVEHTTFICNIITGPSEKPQRHRLRHHNRRHLDRQIEQLKNCEPLKESEVKALCLKAMEILVEESNVQRVDAPVTICGDIHGQFYDMKELFKVGGDCPKTNYLFLGDFVDRGFYSVETFLLLLALKVRYPDRITLIRGNHESRQITQIRTIDRKQEVPHDGAMCDLLWSDPEDIVDGWGLSPRGAGFLFGGSVVTSFNHTNNIDYICRAHQLVMEGFKWMFNNQIVTVWSAPNYCYRCGNVAAILELDENLNKQFRVFKAAPQDSRGAPAKKPPPDYFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CR71</accession>
    <name>A0AAD5CR71_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Knottins-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_006426</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007296">
        <property type="protein sequence ID" value="KAI7745324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CR71"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.10">
        <property type="entry name" value="Knottin, scorpion toxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003614">
        <property type="entry name" value="Knottins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036574">
        <property type="entry name" value="Scorpion_toxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33147">
        <property type="entry name" value="DEFENSIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33147:SF98">
        <property type="entry name" value="GAMMA-THIONIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00304">
        <property type="entry name" value="Gamma-thionin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00505">
        <property type="entry name" value="Knot1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57095">
        <property type="entry name" value="Scorpion toxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Knottins-like" evidence="4">
        <location>
            <begin position="12"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7745324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="90" mass="9972" checksum="DF7B2A988C8BE26B" modified="2024-05-29" version="1">MCKEIGSVKGELCEKASKTWSGNCGNTRHCDDQCKSWEGAAHGACHVRSGKHMCFCYFNCPKAQKLAQDKLRAKELAKEKIEAEKAQPTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CRB0</accession>
    <name>A0AAD5CRB0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_017585</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006887">
        <property type="protein sequence ID" value="KAI7746836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CRB0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035251">
        <property type="term" value="F:UDP-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000040">
        <property type="entry name" value="UDP-glycosyltransferase 76C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48045:SF35">
        <property type="entry name" value="UDP-GLUCURONOSYL_UDP-GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48045">
        <property type="entry name" value="UDP-GLYCOSYLTRANSFERASE 72B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7746836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="368" mass="41821" checksum="13B418A9B007BF35" modified="2024-05-29" version="1" fragment="single">GPLHKIKPSLSISFHKEDTSCITWLDKQPTKSVVYISFGSLATMDAKVLTEIAWGLMKSDQRFLWAVRPGLVSGSEWLELLPEGFIEKTKERGLIVKWAPQKDVLAHFAVGAFWSHCGWNSCLESISLGVVMICQPFDVDQWVNARYVSFIWKIGIELEQVPVFAVGPLHKITPGLSISSHKEDTSCIAWLDKQPPKSVVYISFGSLATMDVKVLTEMAWGLMKSDQRFLWAVRPGLVSGLEWLELLPKSFIEETRERGLIVKWAPQKDVLAHFAVGGFWSHCGWNSCLENQGVNTRYVSYVWKIGIELEQVERGEIERMIRRVMVDDEGEEMRVRVNDMKEMVKEAVENRGSSQESLEGLMDFILSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CRH8</accession>
    <name>A0AAD5CRH8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_005736</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006942">
        <property type="protein sequence ID" value="KAI7746582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CRH8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000595">
        <property type="entry name" value="ATPase family AAA domain-containing protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021911">
        <property type="entry name" value="ATAD3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23075:SF13">
        <property type="entry name" value="AAA-TYPE ATPASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23075">
        <property type="entry name" value="PUTATIVE ATP-ASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12037">
        <property type="entry name" value="ATAD3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042063889" description="AAA+ ATPase domain-containing protein" evidence="6">
        <location>
            <begin position="19"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="7">
        <location>
            <begin position="376"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="23"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="180"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="106"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="54"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="180"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7746582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="621" mass="69715" checksum="AC88232C4B7AB6E2" modified="2024-05-29" version="1">MAASRLASLAAMAAVAAASTTTLSYNDGPSYRFNPFAQSPAPVQPESQPSNVEAKPEVEDSKGGFDPEALERGAKALREINSSPLSKQVFELMRKQEQVRLTELAAEKAHQEAIQAQLDIERQQKLAEEQRNLVQQQSQAKAQLLRYEDDLARKRMQTDHEAQRQHNAELARMQEESLIRKEQARRATEEQIQAQQRQTEKERAEIERETIRVKAMAEAEGRAHEAKLTEDHNRRMLIERVNGEKEKWLAAINTTFSHIEGGVRMLLTDRSKLVMTVGGVTALAAGVYTTREGARVMWGYVNRILGQPSLIRESSMAKFPWSGIVTQGTQKLSNLGKSASVETKHKFGNIILHPSLQRRIEHIARATANTKSHQAPFRNMLFYGPPGTGKTLVAREIARKSGLDYAMMTGGDVAPLGAQAVTKIHEIFDWSKKSKRGLLLFIDEADAFLCERNSTHMSEAQRSALNALLFRTGDQSRDVVLVLATNRPGDLDSAITDRIDDVIEFPLPQEEERFKLLKLYLHKYLYEEDDQKKSKWHSLFAKKTQKIVVKDLSDDVIREAAKKTEGFSGREIAKLIASVQAAVYGRPDCSLDSQLFLEIVDYKVGEHHQRIELANAGSQLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CS59</accession>
    <name>A0AAD5CS59_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_008235</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007208">
        <property type="protein sequence ID" value="KAI7745536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CS59"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="119"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7745536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="140" mass="15586" checksum="A7B582F0754C9C0E" modified="2024-05-29" version="1">MIEFPVRYCLSIPYTPNLPLSLYIERNTPLMEILRVEEMEHGLVPVLFCILLSSTVLANSAEASASSTFRVSQASLSRKILSLPPHPYGHNTPPRIPKPPPPPPHMRSPPPHKRYMPPRECPPPPSTHVPTPCKPHPPTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CTR3</accession>
    <name>A0AAD5CTR3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_016719</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010006739">
        <property type="protein sequence ID" value="KAI7747517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CTR3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009902">
        <property type="term" value="P:chloroplast relocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007623">
        <property type="term" value="P:circadian rhythm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009638">
        <property type="term" value="P:phototropism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05574">
        <property type="entry name" value="STKc_phototropin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000133">
        <property type="entry name" value="LOV domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000265">
        <property type="entry name" value="Putative LOV domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45637">
        <property type="entry name" value="FLIPPASE KINASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 11 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="43"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7747517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="374" mass="42398" checksum="F8097B35C68BCFDE" modified="2024-05-29" version="1" fragment="single">TPDDLWAKHSNIVHPKPHRRDTSVWKAILKVIESGEKLGLKHFRPIKPLGSGDTGSVHLVELCGTGEFFAMKAMDKGVMINRNKVHRACAEREILDVLDHPFLPALYASFQTSTHVCLITDYCSGGELFMLLDRQTMKVLKEDAVRFYAAEVLVALEYLHCQGIIYRDLKPENVLIQSTGHVALTDFDLSCLTSCKPQLLTSITEKKKKHRKNQQTPIFMAEPVRASNSFVGTEEYIAPEIISGVGHSSAVDWWAFGILLYEMFYGYTPFRGKTRQRTFANILHKDLKFPNSIAVSLSGKQLIYRLLNRDPKNRLGSREGASEVKRHPFFRGVNWALVRCENPPKLETPVFEGNEDEKVKEVDPGLKDLENNVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CVQ9</accession>
    <name>A0AAD5CVQ9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_002924</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006579">
        <property type="protein sequence ID" value="KAI7748215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CVQ9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31016:SF20">
        <property type="entry name" value="HEAT-INDUCIBLE TRANSCRIPTION REPRESSOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31016">
        <property type="entry name" value="OS04G0228100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="141"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="282"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="174"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="141"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="288"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="299"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7748215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="320" mass="35907" checksum="31A76A5FABD8D0E9" modified="2024-05-29" version="1">MECVVMQGTGTATYDYTSMGSTNEPGGFWGVLARKAKSILDDDNATRSSQTPTSVKTETVSTSNQVEPHYESFENSRKMDNPKLRKGLDRLTSSLNQIGGTIGNALEEGRTIVDKKTQDIIQETRKLQNRRKVMNDEHNQLQEPQMQSTQQQKTNQETQLKASRDVAIATAAKAKLLLRELKTLKADLAFAKERSSQLEEENKMLREALEKGDHPTDDDDMIRIQLESLLAEKARLANENSVYARENRFLREIVEYHQLTMQDVVYLDEGIEEVTEVNPVASRTLSVSTSPQLPSSPHTSPPPLPSSPHTPLRRTTSEPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CXL8</accession>
    <name>A0AAD5CXL8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Factor of DNA methylation 1-5/IDN2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_012150</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006277">
        <property type="protein sequence ID" value="KAI7749792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CXL8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080188">
        <property type="term" value="P:gene silencing by siRNA-directed DNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045177">
        <property type="entry name" value="FDM1-5/IDN2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005379">
        <property type="entry name" value="FDM1-5/IDN2_XH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21596:SF82">
        <property type="entry name" value="FACTOR OF DNA METHYLATION 5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21596">
        <property type="entry name" value="RIBONUCLEASE P SUBUNIT P38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03469">
        <property type="entry name" value="XH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Factor of DNA methylation 1-5/IDN2" evidence="2">
        <location>
            <begin position="211"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="92"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7749792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="337" mass="39522" checksum="F91D3F9B5461FC15" modified="2024-05-29" version="1">MEEHNKMVSEVRKARGLAVGLAREVDIKNQRIWEMERHIEDISSRLNSMIAEKDRMNHAFSEEMRKMQVIGLQNAKLKSELECQISNMHLLVQESENLKEDVACQRKELEQRANELQKRESQLDNERTSFYIEKEKIAQNPFDSDYSMTVHINGLRDRLTEKEEELHDMDNLNQTLILREHMSNNELQAARKELINVLPHVLESNTIIGLKRMGEKFSAQDWELRSVELSSLWQEKVNNPNWHPFKQAVKDGKVQEIIDEEDSDLVELKSRWGDEACKAVVNALLELNEYNPSGRYVVSELWNFKEGRKASLKEVIECLIQQLKASRSLKRRRDGQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CXZ2</accession>
    <name>A0AAD5CXZ2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Malectin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_008868</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006157">
        <property type="protein sequence ID" value="KAI7750413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CXZ2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000129">
        <property type="entry name" value="Leucine-rich repeat receptor-like kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024788">
        <property type="entry name" value="Malectin-like_Carb-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45631">
        <property type="entry name" value="OS07G0107800 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45631:SF202">
        <property type="entry name" value="SENESCENCE-INDUCED RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12819">
        <property type="entry name" value="Malectin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042155241" description="Malectin-like domain-containing protein" evidence="8">
        <location>
            <begin position="25"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="Malectin-like" evidence="9">
        <location>
            <begin position="34"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF12819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7750413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="458" mass="51729" checksum="9B5039BECAFFC593" modified="2024-05-29" version="1" fragment="single">MEMLRSFYTVCILHVVLTLAMVHAQDDQSGFMSIDCGIPEGSKYTDKKTGINYVSDAGFIESGVSGEILPEYNYGNLDLPLTTLRSFPQNTRNCYTLRPKQGKNNTYLIRVRFYYGNYDSKGQVPEFDLYLGADLWSTVYIPESNENFYELIHVASSDYIHICLVNTGQGNPFISAIELRLFEIIMDAPPSTSLDLYNRYSFGSNDRVRFPDDKYDRMWYSWLLPGTRVVRTTSNTVTPGASNVPSRVMSTAITPTLSTENISLMLNTTIIYKYFFHIHFAEVEILKSNETRGFNIYLNGELWYGPFSPPAYTFRIQSSTIVTGFSKYIFEINKTLNSTLPPLINAMEVYIVKEFQLQQTEDQDAAAIWSIKSTYGLKGNWQGDPCIPHAFLWKGLGCDYSDPQAAKIISLNLSSSGLSGEIATALANLTMLESLDLSYNNLTGDVPEFLAQQDHLRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CZ45</accession>
    <name>A0AAD5CZ45_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Leucine-rich repeat receptor-like serine/threonine-protein kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_001765</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006312">
        <property type="protein sequence ID" value="KAI7749659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CZ45"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000129">
        <property type="entry name" value="Leucine-rich repeat receptor-like kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024788">
        <property type="entry name" value="Malectin-like_Carb-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45631:SF3">
        <property type="entry name" value="OS05G0393100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45631">
        <property type="entry name" value="OS07G0107800 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12819">
        <property type="entry name" value="Malectin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Malectin-like" evidence="10">
        <location>
            <begin position="69"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="9">
        <location>
            <begin position="407"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="550"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF12819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7749659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="550" mass="60442" checksum="039C55A932E2AE73" modified="2024-05-29" version="1" fragment="single">NQRPCQLHYFSPPSPSPNPSSSSSLPCNSPITLLLLNNMFSILSLLFTLSLLPLSLSQSQSPPIKSTLINCGATTSSLISNRSWDPDTKYISTGTPINLTQPTFSPTLSTVRSFPLIQTNLRRPKFCYVVPVYRTGKYLVRTTYYYHGGLNRLGEIPPVFDQIVDGSLWSVVNTTEDYVKGNASYYEGVFKATGKSMSVCLAANQDTVSDLFISALEFVLLANSVYNSTGFDDSSLRLVARHSFGYNGSIVSYPDDKFDRYWEPYGETNPTVSRGANVLINGFWNLPPLKVFQTQLTDNQLKPMELQWPREPLRNSVYYIALYFADDSGTSSRFMNITINNVTFYDNLRVPQTGVALFANRWPLAGPTKIVLTPAAGSTLGPLINGGELFEVIPVGGRTHTRDLIGLDGLKNSFRNPPFDWNGDPCLPKENSWTGITCSDGSNIRVTGLNLTGMGLSGSLSPNVANLTALNDLWLGNNNLSGSIPDLSTLKMLKTVHLEDNRFNGPFPSSLANIDRLQELFLQNNNLTGQVPSSLKAKLGSNLIVDPQNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D0Z5</accession>
    <name>A0AAD5D0Z5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_019104</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006223">
        <property type="protein sequence ID" value="KAI7750080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D0Z5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048040">
        <property type="term" value="F:UDP-glucuronate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042732">
        <property type="term" value="P:D-xylose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044516">
        <property type="entry name" value="UXS-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43078:SF6">
        <property type="entry name" value="UDP-GLUCURONIC ACID DECARBOXYLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43078">
        <property type="entry name" value="UDP-GLUCURONIC ACID DECARBOXYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7750080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="155" mass="17522" checksum="37DAEDFBBA27207A" modified="2024-05-29" version="1">MNSDLGLSKEKMVDLDIDIGDEVGFGDQISRVGHGDDRFRLLGFRLKKVMAMIDHLALASATVVDVGNDSVLFLILMLRQPLFIKVRQQYGPVVVQSTRKVVRVISCYNEGKRTTETLAMDYHRAAADELRLARIFNTYGPIMRDDGFLVSLRFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D1X3</accession>
    <name>A0AAD5D1X3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">FYVE-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_002005</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005878">
        <property type="protein sequence ID" value="KAI7751545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D1X3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11526">
        <property type="entry name" value="SYLF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000151">
        <property type="entry name" value="Zinc finger family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051702">
        <property type="entry name" value="SH3_domain_YSC84-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007461">
        <property type="entry name" value="Ysc84_actin-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15629:SF2">
        <property type="entry name" value="SH3 DOMAIN-CONTAINING YSC84-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15629">
        <property type="entry name" value="SH3YL1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04366">
        <property type="entry name" value="Ysc84"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FYVE-type" evidence="6">
        <location>
            <begin position="140"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="17"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="43"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7751545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="450" mass="48928" checksum="E1CBBD40CDA97C1B" modified="2024-05-29" version="1">MEKNKYFIDDFTDCEWPAAVPQDQPTFHHPTGHNPPLLSEDYNSGDDDDDSQCPDDVPEVNLKNVLTGIVAIVTGRNKDTTQHTLPASNLAFIGSGKDGDTYVHPSVYIPSAPPLLEPTGVNYNAYKQVLEAEPPDWLRDSTAKVCMQCSAPFTALTRGRHHCRFCGGIFCRACSKGRCLLPVKFRERNPQRVCDTCYDMLDPLQGVLINTISNAVQVAKHDVMDWTCTRGWLNLPVGLSMEHEIYKSSNTLRNYCQVARLNPERSIPAAVLKGAKGLAILTVAKAGMLLTYKLGTGLVMSRRADGSWSAPSAIISAGLGWGPQVGGELMDFIIVLHDFKAVKTFCSRMHFSLGAGCSAAAGPVGRVLEADLRAGDKGSGMCYTYSCSKGAFVGVSLEGNIVATRMDTNLQFYGDPYLTTGDILLGTVDRPKAAEPLYAALEDLYSRLVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D2J3</accession>
    <name>A0AAD5D2J3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SAC9 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_003086</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006058">
        <property type="protein sequence ID" value="KAI7750921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D2J3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057554">
        <property type="entry name" value="SAC9_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24765">
        <property type="entry name" value="SAC9_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAC9 C-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF24765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7750921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="89" mass="9913" checksum="1E0605C85B3FD74A" modified="2024-05-29" version="1">MVVVAEYRLPEVRVGTAMYFDFPRPVTACRVSFRLLKDVASFANDPADKDDSSDFQGPLAAGLSLSSRIKLYYYADPYELGKWAILSAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-10-08" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D2K6</accession>
    <name>A0AAD5D2K6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_024830</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005619">
        <property type="protein sequence ID" value="KAI7752748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd21618">
        <property type="entry name" value="RRM_AtNSRA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10501">
        <property type="entry name" value="U1 SMALL NUCLEAR RIBONUCLEOPROTEIN A/U2 SMALL NUCLEAR RIBONUCLEOPROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="57"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="6">
        <location>
            <begin position="316"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="149"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="190"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7752748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="377" mass="42133" checksum="46163C7CB470FA59" modified="2024-05-29" version="1" fragment="single">VGARPEPSSNGYPSTWEDSYLIGRRDVLHGTGSGIPDILNDRPDSLRKADGIGRESNVLFVDALPSDCSRREVSHLFRPFPGFQEIRLVHKEPRHSTDKPMVLCFVEFSDSSRALTALEALQGYKFDNKKPDSPALRIHFARFPFQLPSDDARIPSQPAPNGTRVLSQPPLDSAAYPSKLSHDGGHFPSQLRSDSSVFTSKLPPDSAHFSSKLPPDGSHYSSELPPDDARFPSQLPPNNDHSPFQLPPNREEQNLANLREKELAVQDEEPVVMKKNFKGGSSKKKESEEANKNKVKKFCIKCKQNHYGECYNFKGCYICGKTGHIGKECTSKPRLCFGCGEPGHKHSXXXXLPQWPEAREKRQERGPPKDITVVLLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D306</accession>
    <name>A0AAD5D306_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_032476</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005964">
        <property type="protein sequence ID" value="KAI7751146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D306"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34555:SF7">
        <property type="entry name" value="DUF3741 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34555">
        <property type="entry name" value="INTEGRAL MEMBRANE HEMOLYSIN-III-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7751146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="108" mass="11889" checksum="2C7A01AD8BF7766D" modified="2024-05-29" version="1">MKVALRDVQNDNSNLMHKAHSQNLIPVEGQLVGDACKTIRAKRLALDCASSPILTNNGAHEQLNYPRRKSEFEGNKEKSVGHPKSGSLAQIQQEASQISLNADFMTTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D3Q3</accession>
    <name>A0AAD5D3Q3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_011595</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005586">
        <property type="protein sequence ID" value="KAI7752879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D3Q3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47818:SF2">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47818">
        <property type="entry name" value="RNI-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7752879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="294" mass="33711" checksum="1E8FE49B154732FD" modified="2024-05-29" version="1" fragment="single">MPEVPSLITLCIGAIRDAILDENGNLFYVYQLPSELFDQLLSNLPPLGLQNLQDAMPSGSSSENWFSDTGIRPSRKRKRYDNFDIMWRARYKLRWPIVEHDECTADWQQIYWEKHLQNCLDATAETVSITRYDGSLGDVEIADALLKYVSYEGHVSRSRSHSKLAFHCERFGPYARRLRLQSVHCVADIGHLLRNCQLEYLEIHWLKSKEQVDGLCKLLEKKKDTLASLEFVHCKLPANLVTAICESLHVKGFETNVIKSFSIKRSSFLACSYFPLPVGLESLLITTRCVMCMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D4R5</accession>
    <name>A0AAD5D4R5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DCD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_010919</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005600">
        <property type="protein sequence ID" value="KAI7752839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D4R5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034976">
        <property type="term" value="P:response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.590.10">
        <property type="entry name" value="ph1033 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013989">
        <property type="entry name" value="Dev_and_cell_death_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044832">
        <property type="entry name" value="NRP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46034">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46034:SF32">
        <property type="entry name" value="DCD DOMAIN-CONTAINING PROTEIN NRP-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10539">
        <property type="entry name" value="Dev_Cell_Death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00767">
        <property type="entry name" value="DCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51222">
        <property type="entry name" value="DCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DCD" evidence="2">
        <location>
            <begin position="173"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="122"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="130"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="149"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7752839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="308" mass="34785" checksum="D13A36087FE97861" modified="2024-05-29" version="1" fragment="single">FSDQLRVHTNNLANLSLNDDSIWSTSYVAKRPEKDRRNFDIRVGGSSNNIGTLNSDFNGFNYDWKFGSSGVIGSSSPNPVGDYGINGGFNKGVYLNSGFNVNVNVNQNFNNQINSNVKVVNDKEKQSVRNNKHGKNKKNRGNNNNNVNKNEKDGGVDKRFKTLPPTESLPRDETVGGYIFVCNNDTMQENLKRELFGLPGRYRDSVRQITPGLPLFLYNYSTHQLHGIFEAASFGGTNIDPTAWDDKKKQGESSFPAQVRVKTRNLYEPLEEDSFRPILHHYDGPKFRLELNIPEALSLLDVFEEKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D554</accession>
    <name>A0AAD5D554_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">JmjC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_029642</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the JARID1 histone demethylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010003506">
        <property type="protein sequence ID" value="KAI7754441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D554"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041667">
        <property type="entry name" value="Cupin_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12461:SF99">
        <property type="entry name" value="BIFUNCTIONAL PEPTIDASE AND (3S)-LYSYL HYDROXYLASE JMJD7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12461">
        <property type="entry name" value="HYPOXIA-INDUCIBLE FACTOR 1 ALPHA INHIBITOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13621">
        <property type="entry name" value="Cupin_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="JmjC" evidence="2">
        <location>
            <begin position="121"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7754441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="283" mass="31566" checksum="4D66242E5F0DCE1D" modified="2024-05-29" version="1" fragment="single">RDLSLGTTQTIDRLDTPPTPLHFLRHYLSQNKPFILSPSFSSTLHWPATTLWSSTPYLLNTLHSTTVSLHRTPTGQADSLTPHPHTPTSLCFASADVTLTSFPNAVDSVIGSCKNNNGGCVGYLQQQNDCFRGEYGELNGDCDLDVGWATEAIGAGPEAVNLWIGNEMSETWFHKDCFENLYAVVTGEKHFLLLPPTDVHRMYVKEYPAAKYEYSEFYYVPLSSFLCSHTRPGSLKVLVYEGVKTTTFSSKHVPKIGELITADIVLTRYDVLREDISHDTDRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D5F9</accession>
    <name>A0AAD5D5F9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Secretory carrier-associated membrane protein</fullName>
            <shortName evidence="7">Secretory carrier membrane protein</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_025164</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 7">Probably involved in membrane trafficking.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Cytoplasmic vesicle</location>
            <location evidence="7">Secretory vesicle membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the SCAMP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005696">
        <property type="protein sequence ID" value="KAI7752406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D5F9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055038">
        <property type="term" value="C:recycling endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032588">
        <property type="term" value="C:trans-Golgi network membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030658">
        <property type="term" value="C:transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007273">
        <property type="entry name" value="SCAMP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10687:SF76">
        <property type="entry name" value="SECRETORY CARRIER-ASSOCIATED MEMBRANE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10687">
        <property type="entry name" value="SECRETORY CARRIER-ASSOCIATED MEMBRANE PROTEIN SCAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04144">
        <property type="entry name" value="SCAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="111"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="143"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="185"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="15"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7752406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="246" mass="27861" checksum="F7E1B8C30D97C885" modified="2024-05-29" version="1">MGNDYDSNPFAGEDLSTKEKELEAKEAELNQREEELKKKEDTVTRGGIGVEEKNWPPFFPLIHHDIPKEIPVHLQRTQYVAFTTLLGLCVCLIWNLVAVTTAWFEGADSEIWFLAVIYLLIGIPGAYILWYRPLYRAMRTNGALMFVLFFFTYSCHLFFCVYASIAPPLIFEGESLTGILPALDILPVNGLVGCVYLIGFALFALESLISLWVIQDVFRYFCSSGRAEEAKREVRRSSMMVALGHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D5T9</accession>
    <name>A0AAD5D5T9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">sucrose synthase</fullName>
            <ecNumber evidence="2">2.4.1.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_023561</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>an NDP-alpha-D-glucose + D-fructose = a ribonucleoside 5'-diphosphate + sucrose + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16241"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17992"/>
            <dbReference type="ChEBI" id="CHEBI:37721"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:76533"/>
            <dbReference type="EC" id="2.4.1.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 1 family. Plant sucrose synthase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.13" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010005175">
        <property type="protein sequence ID" value="KAI7753887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D5T9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016157">
        <property type="term" value="F:sucrose synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005985">
        <property type="term" value="P:sucrose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1230:FF:000001">
        <property type="entry name" value="Sucrose synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.330:FF:000001">
        <property type="entry name" value="Sucrose synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000368">
        <property type="entry name" value="Sucrose_synth_GT-B1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012820">
        <property type="entry name" value="Sucrose_synthase_pln/cyn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056736">
        <property type="entry name" value="SUS_EPBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056735">
        <property type="entry name" value="SUS_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45839">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45839:SF13">
        <property type="entry name" value="SUCROSE SYNTHASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00862">
        <property type="entry name" value="GT-B_Sucrose_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24862">
        <property type="entry name" value="SUS_EPBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24861">
        <property type="entry name" value="SUS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sucrose synthase N-terminal" evidence="7">
        <location>
            <begin position="1"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="Sucrose synthase EPBD" evidence="8">
        <location>
            <begin position="145"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="Sucrose synthase first GT-B" evidence="6">
        <location>
            <begin position="292"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF24861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF24862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7753887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="492" mass="55737" checksum="62BE4FD1073788A8" modified="2024-05-29" version="1">MRERVEQTLSAHRNEIVSLLSRYVAQGKAILQPHQILDELENIIGDEASRQKLTDGPFGDALRTAQECIVLPPFVALAVRPRPGVWEYVRVDAYQLSVQQLTASEYLVFKEELAGEPNSSYVLELDFEPFNASFPRPNRSESIGNGVQFLNRHLSSSMFRSKDCLEPLLDFLRTHKHDGHVMMLNDRIQSINRLQSSLSKADDYLSKLPSDTAYSEFQYDLQGMGFERGWGNNAERTLEMMHLLSDILQAPDPSILETFLGRIPMVFNVVILSIHGYFGQANVLGLPDTGGQDAASEIAAELHGAPDLIIGNYSDGNLVASLLSYKMGVTQCNIAHALEKTKYPDSDLYWKKFDEKYHFSCQFTADLLAMNNADFIITSTYQEIAGTKNTVGQYESHSSFTLPGLYRVVHGIDVFDPKFNIVSPGADMTIYFSYSEKEKRLTSLHAKLEKLLFDPEQNEEHMRETRRYLEMFYILKFRDLVKSVPVAIDDEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D618</accession>
    <name>A0AAD5D618_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Auxin response factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_019628</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Auxin response factors (ARFs) are transcriptional factors that bind specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs).</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimers and heterodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ARF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005631">
        <property type="protein sequence ID" value="KAI7752721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D618"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009734">
        <property type="term" value="P:auxin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10017">
        <property type="entry name" value="B3_DNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.1040:FF:000001">
        <property type="entry name" value="Auxin response factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000047">
        <property type="entry name" value="Auxin response factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.330.10">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010525">
        <property type="entry name" value="ARF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044835">
        <property type="entry name" value="ARF_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033389">
        <property type="entry name" value="AUX/IAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003340">
        <property type="entry name" value="B3_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015300">
        <property type="entry name" value="DNA-bd_pseudobarrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053793">
        <property type="entry name" value="PB1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31384:SF173">
        <property type="entry name" value="AUXIN RESPONSE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31384">
        <property type="entry name" value="AUXIN RESPONSE FACTOR 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06507">
        <property type="entry name" value="ARF_AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02309">
        <property type="entry name" value="AUX_IAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02362">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01019">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101936">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50863">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51745">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0927">Auxin signaling pathway</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 8" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TF-B3" evidence="11">
        <location>
            <begin position="93"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="PB1" evidence="12">
        <location>
            <begin position="917"/>
            <end position="1001"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="533"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="580"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="632"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="481"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="533"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="587"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="632"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="658"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="676"/>
            <end position="687"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7752721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="1030" mass="114956" checksum="351D13700C9268C0" modified="2024-05-29" version="1">MKISANSSGSSLTTANPVDGGGEKNRIINPELWQACAGPLVNLPVAGTHVVYFPQGHSEQVAASMKKDVDAQIPSYPNLQSKLICILHKITLHADTETDEVYAQMTLQPVPSDFNMQPPAQELVARDLHDNVWTFRHIYRGQPKRHLLTTGWSLFVSGKRLFAGDSVLFIRDEKQQLLLGIRRANRQPTNISSSVLSSDSMHIGVLAAAAHAAANNSPFTVFYNPRASPSEFVIPLAKYYKAVCSNQISLGMRFRMMFETEESGTRRYMGTITGISDLDGVRWKNSQWRNLQVGWDEATAGERRNRVSIWEIEPVTAPFFMCPTPPFYQTKRPRQGMFGDESADFDNVFKRTMPWLGEDMSMKDPGLSLAQWMNMQQNPSLANQIQPGYLNSLAGSVLQNYGGPQVLTQNNIQFNSLNGQRSTSQLDQLQKLPQPATTNPLAMMMQPQQLTDLTQQTVRQNLINQSLPQNQVQSQIQPQNILQLQQQQQLQQQQLQQQQQLQQQQHQQQQQQQQQQLQQLQQHQQLQQHQQQQQMMAVNQSQSQQKIPLPSQFQDPQYGQKLQMPENQIQLLQKIHQQQQSLLAQQGHPQEQSFTRSMSTTQMTETPSQTPVTSVPQQMSRNNNEQNFRFSHLSQQPKLQHELSGQMTGPAPVPANQLSTNSSSLLTGTGSDDVPSCSTSPSTNNCPNVGPTMINGRPYQRATVVGEDIIQASTHLMNTSGLETISANENFGKELQQKVDVKPSVNVSKIQNQGFLAPRTYLQNLGAPQMDYLDSSSSATSVCISQTDVQLQPHSSNHMSFNSQPLPFRDTVQDEEARNDPRTDIPFGVNMSSVIPDPLITKGMMGSENNLSSGGGLISSYENPAKETQPELSSSMVSQSFGVPDMTFNSIDSTINDDTLLSNGVWAPPPQPFQRLRTYTKVYKRGAVGRSIDIGAYSGYDELKQDLARRFGIEGQLEDRQRVGWKLVYVDHENDVLLVGDDPWEEFVNCVRCIKILSPQEVQQMSLDGDFGGNSVLQNQACSSSGGGQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D824</accession>
    <name>A0AAD5D824_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_010113</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005496">
        <property type="protein sequence ID" value="KAI7753245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D824"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7753245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="53" mass="5642" checksum="5889F2FEF3C24A34" modified="2024-05-29" version="1" fragment="single">MVITTNVNNSITIQVTPSGSFGTFFIVADVQGHLAPSSTDSGSSGNIIFDFYW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D927</accession>
    <name>A0AAD5D927_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_010954</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010003822">
        <property type="protein sequence ID" value="KAI7754386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D927"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051266">
        <property type="entry name" value="CLCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057427">
        <property type="entry name" value="WAV3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10579">
        <property type="entry name" value="CALCIUM-ACTIVATED CHLORIDE CHANNEL REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10579:SF59">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE EDA40-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25243">
        <property type="entry name" value="WAV3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="2">
        <location>
            <begin position="109"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="3">
        <location>
            <begin position="296"/>
            <end position="489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7754386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="664" mass="74042" checksum="60C3359DC901AC90" modified="2024-05-29" version="1">MSQLTQPKKMVSGWRKVFCTSIPHEPNPNPIEDHEDDHQLSNCTRFRFFSSSSSNPSTPRLRCRTTAAVPSPSPSTPLSPRVFNTPSPCSPSSFSFIKSSLRLRPQKRCGICLQSMKKGNGTASFTAECSHAFHFPCVSDYVTTQGSLACPVCSCLWKDMPLLTVNQGRYTNQLHTLCDEENSREKLSTRFIEDVVDAHEYMKEKRRHKRPDLKVYNDDEPLAVLTPKVRFNPIPESDENNDEELEIYNNDASLEVRMSPETAVIAASGRHLTYGIVLNLKAELKKEKPHLRAPIDLVAVIDVTGKTTSENLQKMKRTMRSIVSLLSSSDRLSIVAFSSHSKRLLGLRRMTTSGKRAVLAIVEAMAILEGFSNSNDAVKKAVKVLLDRSENNTLAAVILLSNDRYSHSRFSFPIHSVNLRRDEEYVTRKLIEKVLNVSVQEVRLQLGIVSGTSVISTVLDPKTTSIFGSSTVEIGEMSANEERELLVEIKLPLAAVRVNQVLSVQCSYRESLTQEMIYGQQQTLTVPHPTIVRSSSCPPTIQRLRNMFISARALAEARRLTEGNDLAGACNVLVSAYGLIKRSSGGDNEFVLGLEAELSELQRRRKAQVRARAQETVVCNMDGKGEPLTPTSAWRMADKLAKAAMMRKSVNRVSDLHGFEDARF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DAA4</accession>
    <name>A0AAD5DAA4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_026094</name>
        <name evidence="3" type="ORF">M8C21_026095</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ARG7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000726">
        <property type="protein sequence ID" value="KAI7755886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAMZMK010000726">
        <property type="protein sequence ID" value="KAI7755887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DAA4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009733">
        <property type="term" value="P:response to auxin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003676">
        <property type="entry name" value="SAUR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31929">
        <property type="entry name" value="SAUR-LIKE AUXIN-RESPONSIVE PROTEIN FAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02519">
        <property type="entry name" value="Auxin_inducible"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7755886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7755887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="98" mass="11107" checksum="C356B53DEE509692" modified="2024-05-29" version="1">MGIIRFPCLNGNAKRFSKLQSTCKENQSQVPKGYFAVYVGEVQKTRFVVPLSLLEHPLFQSLLRKSEEEFGFRHPMGGVTIICPEDDFIDITSKLLVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DAN2</accession>
    <name>A0AAD5DAN2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">60S acidic ribosomal protein P0</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_021049</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 6">Ribosomal protein P0 is the functional equivalent of E.coli protein L10.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">P0 forms a pentameric complex by interaction with dimers of P1 and P2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the universal ribosomal protein uL10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000179">
        <property type="protein sequence ID" value="KAI7757113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DAN2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05795">
        <property type="entry name" value="Ribosomal_P0_L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.105.20:FF:000001">
        <property type="entry name" value="60S acidic ribosomal protein P0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.105.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050323">
        <property type="entry name" value="Ribosomal_protein_uL10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001790">
        <property type="entry name" value="Ribosomal_uL10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040637">
        <property type="entry name" value="Ribosomal_uL10-like_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043164">
        <property type="entry name" value="Ribosomal_uL10-like_insert_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043141">
        <property type="entry name" value="Ribosomal_uL10-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030670">
        <property type="entry name" value="uL10_eukaryotes"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45699">
        <property type="entry name" value="60S ACIDIC RIBOSOMAL PROTEIN P0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45699:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00428">
        <property type="entry name" value="Ribosomal_60s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00466">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17777">
        <property type="entry name" value="RL10P_insert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039087">
        <property type="entry name" value="L10E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160369">
        <property type="entry name" value="Ribosomal protein L10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL10-like insertion" evidence="8">
        <location>
            <begin position="113"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="286"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR039087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7757113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="319" mass="33978" checksum="8499162BEAB0F44D" modified="2024-05-29" version="1">MGKVSKADKKIAYDHKLCQLLDEYTQVLVAAADNVGSNQLQNIRQGLRGDSVILMGKNTMMKRSIRMHSEKTGNKAFLNLVPLLVGNVGLIFTKGDLKEVREEVAKYKVGAPARVGLVAPVDVVVPPGNTGLDPSQTSFFQVLNIPTKINKGTVEIITPVELIKKGDKVGSSEAALLAKLGIRPFSYGLVVLSVYENGSVFSPEVLDLTEDDLVEKFALGVSMVTSLALAIHYPTIAAAPHMLINGYKNVLAVAVETEYSFPLADKVKEYLANPSKFAVAALLGGGGDAPAAAAAAPAEEKKEEPDEESDDDLGLGLFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DCR2</accession>
    <name>A0AAD5DCR2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_031435</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000138">
        <property type="protein sequence ID" value="KAI7757307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DCR2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46008">
        <property type="entry name" value="LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-LIKE 1.4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46008:SF25">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="385"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="402"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="630"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7757307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="630" mass="71202" checksum="06729B7E534F7EF2" modified="2024-05-29" version="1" fragment="single">LPHTCQVVHLPAKLSSSPGPGLNETDIVSLLSSYTSVTFNLSHSCSECRKKGRLCDTENERDVYCFDIEKAVTPGREPTPSPNQGKQVRELINILDISFSCGVSVFSYEELEDATQNFDPSHELGDGGFGAVYYDGNISTPICPESFSCPGLGSLKYPFYNGTDRKCGLIKINCTSKEIQFGGWLYEFVGKFEFNHDSDRNPNNIIIYNRTFEQLVIKNKSCEALMYNFTSPSPSPLLYSVSIFPTVTLFKCRKNLTYPQQNKPYFEKHKYNSYNSCKDHDFYYNYINGTVPSDLPHTCQVVYLPAKLSSSRGLDETNLFSLVSSYTSITFNLSKSCSKCQKEGRLCGIEKGRVQCLDVKWVKTDGEQFRIPNLERKVRERTIKILVIAGSVVFLMLSLVLFIIWRHFKRNPFSYVSTRKKSLNLEDISKLQDGRQVAVKKLYENNCNRVQQFRNEVQILTKLRHPNLVVLYGCTSKESCEFLLVYEYVPNGTVADHIHGEQANQSMLTWSVRMNIAIETASALYDSEMRPTMNEVLDVLMDIQDVGRLDTYGSTRDLQAVNALPLSETNDTAGLLKDFRPSPISVINEWQSNTSASTTLSSNGDRLSLKDNIDTYKTHGSKNDKSASIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DD47</accession>
    <name>A0AAD5DD47_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 12">Phenylalanine ammonia-lyase</fullName>
            <ecNumber evidence="6 12">4.3.1.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_021456</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This is a key enzyme of plant metabolism catalyzing the first reaction in the biosynthesis from L-phenylalanine of a wide variety of natural products based on the phenylpropane skeleton.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-phenylalanine = (E)-cinnamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:21384"/>
            <dbReference type="ChEBI" id="CHEBI:15669"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="EC" id="4.3.1.24"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 12">Phenylpropanoid metabolism; trans-cinnamate biosynthesis; trans-cinnamate from L-phenylalanine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the PAL/histidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.3.1.24" evidence="6 12"/>
    <dbReference type="EMBL" id="JAMZMK010000165">
        <property type="protein sequence ID" value="KAI7757185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DD47"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045548">
        <property type="term" value="F:phenylalanine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006559">
        <property type="term" value="P:L-phenylalanine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009698">
        <property type="term" value="P:phenylpropanoid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00332">
        <property type="entry name" value="PAL-HAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.200.10:FF:000009">
        <property type="entry name" value="Phenylalanine ammonia-lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.200.10">
        <property type="entry name" value="Fumarase/aspartase (Central domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="entry name" value="Fumarase/aspartase (N-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001106">
        <property type="entry name" value="Aromatic_Lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022313">
        <property type="entry name" value="Phe/His_NH3-lyase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005922">
        <property type="entry name" value="Phe_NH3-lyase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01226">
        <property type="entry name" value="phe_am_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10362">
        <property type="entry name" value="HISTIDINE AMMONIA-LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00221">
        <property type="entry name" value="Lyase_aromatic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="L-aspartase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00488">
        <property type="entry name" value="PAL_HISTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 11" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-0585">Phenylalanine catabolism</keyword>
    <keyword evidence="8 12" id="KW-0587">Phenylpropanoid metabolism</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7757185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="365" mass="39347" checksum="607CFA82B4FA39B0" modified="2024-05-29" version="1" fragment="single">MLVRINTLLQGYSGIRFEILEAITKFLNNNITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAVGPAGETLNAESAFAKAGVEGGFFELQPKEGLALVNGTAVGSGMASMVLFEANVLALLSEVLSAIFAEVMQGKPEFTDHLTHKLKHHPGQIEAAAIMEYILDGSDYVKAAQKVHEMDPLQKPKQDRYALRTSPQWLGPQIEVIRSATKMIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIAAIGKLMFAQFSELVNDFYNNGLPSNLSGGRNPSLDYGFKGGEIAMASYCSELQFLANPVTNHVQSAEQHNQDVNSLGLISARKTAEAVDILKLMSSTYLVALCQSID</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DDL2</accession>
    <name>A0AAD5DDL2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">CRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_019746</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000101">
        <property type="protein sequence ID" value="KAI7757549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DDL2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000373">
        <property type="term" value="P:Group II intron splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.110.60:FF:000002">
        <property type="entry name" value="CRS2-associated factor 1, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.60">
        <property type="entry name" value="YhbY-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045278">
        <property type="entry name" value="CRS1/CFM2/CFM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001890">
        <property type="entry name" value="RNA-binding_CRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035920">
        <property type="entry name" value="YhbY-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31846:SF10">
        <property type="entry name" value="CHLOROPLASTIC GROUP IIA INTRON SPLICING FACILITATOR CRS1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31846">
        <property type="entry name" value="CRS1 / YHBY (CRM) DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01985">
        <property type="entry name" value="CRS1_YhbY"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM01103">
        <property type="entry name" value="CRS1_YhbY"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75471">
        <property type="entry name" value="YhbY-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51295">
        <property type="entry name" value="CRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="3" id="KW-0934">Plastid</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="6 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="CRM" evidence="13">
        <location>
            <begin position="179"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="CRM" evidence="13">
        <location>
            <begin position="350"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="domain" description="CRM" evidence="13">
        <location>
            <begin position="563"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="106"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="19"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7757549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="718" mass="82373" checksum="3771D35A5C0A5B4D" modified="2024-05-29" version="1">MSSTMFLSPTMLSKPSNFPKTNNPTNQQTNPKSNSIPTPNKNHEFNPNPLNSATKTAPWMKTPLVLEPNQVIDFTKPKNKKKSPDNTKDEKILTQKVSGGRGKHAMKKILYSIDNLEQETQELEDTHKHMEFDLSLCKLGDDKDGRNRKWFGRKLPWVREERMVFRRMKKEKTVTVADLSLDEGLLERLRGEARKMRNWVKVKKAGVNEGVVEQIGVIWDSDELAMVKFDVPLCRNMDRAREIVEIKTGGLVIWSKKDTLVVYRGCNYNDYKHVTGTIYQTVGGHDDKEETMPVQGSLFEREANRLLDGLGPRFIDWWMPKPLPVDADLLPEVVPGYKPPSRLYPLSGRSKLTDTELTHLRKQAYHLPTHFVLGRNRNLQGLAAAILKLWEKCRIAKIAVKWGIPNTSNEQMASELKLLTGGVLLLRNKFYIILYRGKDFLPPGVADLVTNREMEIKRFQLQEETARANTVEAFDYLDQPSSDCSTVGTLKEFQTIQSEHEGFKAGISEAGIQVEAEKLKLEKEIRIQERKYLILKMKINKASKELAKLNSAWEPVEPNEDREIITSEERECLRKMGLKMDSTLVLGRRGVFDGVIEGLHQHWKHREIVKVVTLQRVFSRILYTAQCIEAESGGVLVSIEKIKLGYAIIMYRGKNYKRPSKLSRNLLTKREALQKSLEMQRIGSLNFFANMRQQAIYELKCKLDALKYTDMAENKLTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DED0</accession>
    <name>A0AAD5DED0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Folate receptor-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_031721</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000051">
        <property type="protein sequence ID" value="KAI7757852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DED0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053305">
        <property type="entry name" value="Folate-binding_rcpt-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018143">
        <property type="entry name" value="Folate_rcpt-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37390:SF1">
        <property type="entry name" value="FOLATE-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37390">
        <property type="entry name" value="OS02G0592500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03024">
        <property type="entry name" value="Folate_rec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="248"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="Folate receptor-like" evidence="5">
        <location>
            <begin position="70"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="272"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7757852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="302" mass="33497" checksum="13714C91C9EFC39E" modified="2024-05-29" version="1">MYSYSYWYLPQRRMKLDLYSLLFLLLQILACFNRLLPFASAKSDGVCVSPGGRFPPYTIQGKPPRRVSKGPTDLTLCRVFRKNTCCDVTQTHPALLAVRRLASSGEASDECLHLWELLECSICDPHVGVQSGPPVICASLCDRIYDACSNAYFAMDGKNQVLVPCGISDTVCGRASEWVSNGTELCKASGFSVKPSNDLKETFCYGGKASLDSVVDSWRTSHARDAKSSGYLYDFQQWVREMPINEQVSWAVGGLVLTAGLLFVSKRKSHNQRQRQAAIQRTARRLGGKTSPKYPASQANRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DFX5</accession>
    <name>A0AAD5DFX5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_018692</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010000036">
        <property type="protein sequence ID" value="KAI7757971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DFX5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009742">
        <property type="term" value="P:brassinosteroid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14137">
        <property type="entry name" value="STKc_GSK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000009">
        <property type="entry name" value="Glycogen synthase kinase-3 beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000082">
        <property type="entry name" value="Shaggy-related protein kinase kappa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050591">
        <property type="entry name" value="GSK-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039192">
        <property type="entry name" value="STKc_GSK3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24057">
        <property type="entry name" value="GLYCOGEN SYNTHASE KINASE-3 ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24057:SF42">
        <property type="entry name" value="SHAGGY-RELATED PROTEIN KINASE ETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="40"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7757971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="384" mass="43277" checksum="1DFB765329AC5C92" modified="2024-05-29" version="1">MADDKEMSTKVVDGNGAETGHIISTTIGGNNGEPKQTVSYMAERVVGAGSFGIVFQAKCLETGETVAIKKVLQDKRYKNRELQLMRTMDHPNVVSLKHCFYSTTSKDELFLNLVMEYVPETIFRVLKHHSDSRQRMPLIYVKLYTYQIFRGLAYMHMVTGVCHRDLKPQNVLVDPVTHQVKICDFGSAKMLVRGEANISYICSRFYRAPELIFGATEYTTSIDIWSAGCILAELLLGQPLFPGENAVDQLVEIIKVLGTPTREELRCMNPNYTDFRFPQVKAHPWHKVFHKRMPPEAIDLASRLLQYSPSLRCNALEAIAHPFFDEIRQPNARLPSGRPLPPLFNFKQELSAASPELVNRLIPDHVKRQMGLQFVPPGGGAVGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G4C2</accession>
    <name>A0AAD5G4C2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_001812</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011234">
        <property type="protein sequence ID" value="KAI7728334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G4C2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042170848" evidence="1">
        <location>
            <begin position="23"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7728334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="154" mass="17014" checksum="7357F6372AC55726" modified="2024-05-29" version="1" fragment="single">KATFLCLILISIATRELHFCLCLSLSLDLSFPLTPPHTDGIVIGNGNGLRLRRSDHNYSVFVALASRPSNKNQSLDNKVDEVSGGRIATAAATPFGQVDEVRLPHNRRAHHHTPARKETRDSDVVVTKRTVTCDTDRLAVQDRRACYITNKVNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G5U6</accession>
    <name>A0AAD5G5U6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_001081</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011193">
        <property type="protein sequence ID" value="KAI7728563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G5U6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009725">
        <property type="term" value="P:response to hormone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009744">
        <property type="term" value="P:response to sucrose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004254">
        <property type="entry name" value="AdipoR/HlyIII-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20855">
        <property type="entry name" value="ADIPOR/PROGESTIN RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20855:SF115">
        <property type="entry name" value="HEPTAHELICAL TRANSMEMBRANE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03006">
        <property type="entry name" value="HlyIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="62"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="141"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="177"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="204"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="236"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="269"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="308"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="306"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="310"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR604254-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7728563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="336" mass="38436" checksum="EBDEE5C35A64C127" modified="2024-05-29" version="1">MNKSSIKSSKIETETEIVYPLMSFEDLPEYMKDNEFILNHYRVNWPLKQAFFSLFHWHNETLNVWTHLIGFFVFVGLTIGNMMQVPEVMDFLNALTWSVPLNPGANASNISAMDTPKLVDLMHSPPQDMERPPLLMAETRWPFFVFLAGAMFCLLSSSTCHLFGCHSHRLSLQLLQLDYVGITVMIITSFFPPIYYIFQCNPLWQFVYLGGVTTMGVFTIITLLSPALSSGKFRSFRAFLFMSMGLFGLIPAVHAVILNWDKPQFNATLVYESVMALSYLIGTMFYVTRIPERWKPGWFDLAGHSHQIFHCFVIMGALAHYNAAQLFLESWGRAGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G684</accession>
    <name>A0AAD5G684_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Patellin-3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_009536</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the patellin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010779">
        <property type="protein sequence ID" value="KAI7730450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G684"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.525.10:FF:000022">
        <property type="entry name" value="SEC14 cytosolic factor family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.680">
        <property type="entry name" value="GOLD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011074">
        <property type="entry name" value="CRAL/TRIO_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036598">
        <property type="entry name" value="GOLD_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044834">
        <property type="entry name" value="PATL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056794">
        <property type="entry name" value="PATL1-6_C_GOLD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45932">
        <property type="entry name" value="PATELLIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45932:SF6">
        <property type="entry name" value="PATELLIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03765">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25099">
        <property type="entry name" value="GOLD_PATL1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01100">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46938">
        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101576">
        <property type="entry name" value="Supernatant protein factor (SPF), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50866">
        <property type="entry name" value="GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="10">
        <location>
            <begin position="110"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="GOLD" evidence="11">
        <location>
            <begin position="261"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7730450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="400" mass="46557" checksum="04B96CF300718CD7" modified="2024-05-29" version="1">MLSFKQETNKIKDLPPLEQLSLTDLKHMVQQAITNKDFYFTSNPEEPTSEIISNHEEITIWGIPLLKDERTDVVLLKFLRAREFKVNESFNMLKNTLRWRKAFSIDELVDESLGDDLDKVVFMHGNDKEGHPVCYNVYGEFENKELYLKTFADEEKRTRFLKWRIQFLERSIRKLDFRPGGISTIFQVSDLKNSPGPGKKELRWATKQALQLLQDNYPEFVSKQVFINVPWWYLAFYSMMSPFMTQRTKSKFVFASPARTAEVLFKYVSPEHVPIQYGGLSVDYCDCNPEFTIDDSAAVVTVKPMTKQTVEIIVNEKCTLVWELRVVGWEVSYGAEYVPNNGDNYTINVQKPKKMAPTDEPVISHSYKINELGKLLLTIDNPTSKKKKLLYRFKVEPLPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G6H7</accession>
    <name>A0AAD5G6H7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_031791</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 16">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the PP2C family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="4"/>
    <dbReference type="EMBL" id="JAMZMK010010822">
        <property type="protein sequence ID" value="KAI7730212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G6H7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000020">
        <property type="entry name" value="Probable protein phosphatase 2C 42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000222">
        <property type="entry name" value="PP2C_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01032">
        <property type="entry name" value="PPM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 12" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="13">
        <location>
            <begin position="44"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7730212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="369" mass="41277" checksum="49A2F8E4187F8898" modified="2024-05-29" version="1">MFKKLMNCCSVCWFGHDHKENLVGVNGDFGKDGLLWYHDIGKHVYGEYSMAVVQANQVLEDQSQIESGPFGTFVGVYDGHGGPDVARYICDHLFRHFQANTTENHGIVTPETIKRAFLETENGFTSLVSKMWNVRPSIATVGSCCLVGVVYQQTLFVANLGDSRLVLGRKIGSSVTAIQLSKEHNANLENIRRELRDLHPHDPQIVVMKHGVWRVKGIIQVSKSIGDAYMKHARYNKEPIAAKFRLPEPTVMPILSATPSVVSHPLQPGDSFLIFASDGLWEQLSNEEAVDIVHKSPRAGIAKRLVKAALHEAAKKREMRYSDLRKIDKHVRRHFHDDISVVVLFLNHDLISKGSAYGPSVSLRSALEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G6V8</accession>
    <name>A0AAD5G6V8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_002989</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCA family. CPR flippase (TC 3.A.1.211) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010651">
        <property type="protein sequence ID" value="KAI7730779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G6V8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005319">
        <property type="term" value="F:lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026082">
        <property type="entry name" value="ABCA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19229:SF154">
        <property type="entry name" value="ABC TRANSPORTER A FAMILY MEMBER 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19229">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER SUBFAMILY A ABCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12698">
        <property type="entry name" value="ABC2_membrane_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="381"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="420"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="460"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="487"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="domain" description="ABC-2 type transporter transmembrane" evidence="8">
        <location>
            <begin position="288"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="7">
        <location>
            <begin position="644"/>
            <end position="713"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7730779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="754" mass="83916" checksum="3CA3F698D2234527" modified="2024-05-29" version="1">MKEFDRSYMVDERSGRDIMVVWLYGEEDDIGGAIWLDDKGKLAKVVYMLLNHSTGFPYELRQAENNTWVVHGTTEDVVSGERSYNIGNKHWHAMIVMSSRKSVHDGDGQCERVCGIQYSDLDQVATCPIPNPPEWPPMLQLPDPRFRAVRTNSISFGDLPSEGCRDTGSCPVTVLMTGSNQSLGESLGRNMFPSSSNFNSSNGLAGVVLGSASQTDITNFLDSAFYSDFPIYYVQSQCGANSTFSLSVPIASLDLDKEIRCAQGQHLWRNSSSDINNELYAGYRKGNPEEKINEILAGYDFMNSNMNNYNVTVWYNSTYKNDTGNGPLGIVRVPRSINLVSNAFLQLLVGPSTQMLFDFIKEMPKPETEIRLDFSSLLGPLFFTWVILQLFPIVLTGLVYEKQQNLRIMMKMHGLGDGPYWIISYAYFLAISLVYMFCFVAFGSAVGLKFFTLNDYSIQFVFYFIYINLQISMAFLVAALFSNLKTAAVVGYITVFATGLLGGFFFQFFLQDKSFSRVWIVVMELYPGFSLYAGLYEFSEYAFTGNYIGTDGMHWGDISDSNNEMRHAVSSGSGARKSPLFFLQKFKKNSSTSFRKPSLQRQGSKRERVEQSILEQDTSHAIVCDNLKKVYPGRDGNPEKFAVRGLSLALSQGECFGMLGPNGAGKTSFINMTRSGWMIGLIKPSSGTAYVQGLDITTNMDQIYANMGAVEESLKSVNLFNGGVADKQAGKYSGGMKRRLSVAISLIGDPKPTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G8U4</accession>
    <name>A0AAD5G8U4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_028714</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010161">
        <property type="protein sequence ID" value="KAI7732777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G8U4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd12203">
        <property type="entry name" value="GT1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000061">
        <property type="entry name" value="Trihelix transcription factor GT-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000092">
        <property type="entry name" value="Trihelix transcription factor GT-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044822">
        <property type="entry name" value="Myb_DNA-bind_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21654">
        <property type="entry name" value="FI21293P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21654:SF59">
        <property type="entry name" value="TRIHELIX TRANSCRIPTION FACTOR DF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13837">
        <property type="entry name" value="Myb_DNA-bind_4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="62"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="455"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="156"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="241"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="362"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="570"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="20"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="30"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="52"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="241"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="362"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="372"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="390"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="572"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="589"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="601"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="624"/>
            <end position="633"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7732777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="633" mass="70575" checksum="EC79992F677F3729" modified="2024-05-29" version="1">MMLQGVANTGHDAPDHHSAQQHQQPNDNISAAAAAAATAAASADLAASGFSEDERGRVDDGSRNSGGNRWPRQETLALLKIRSDMDVAFRDSSLKGPLWDEVSRKLAELGYHRSAKKCKEKFENVYKYHKRTKDGRTSKADGKTYRFFDQLQALEGSPSASSHHQQPIPLPPQPVVSNTPQVVSQMSNTQIPSVVTPVSMPVSVSQPNNVDPISVAAPAVAMPTMNINHVGGFPFSQLNMSASTNSSSSSTSSEDELPERRGNRKRKWKEFFGKLMKEVVDKQEEMQAKFLDTLERRERDRMAREEAWRMQEMAKMKREHELLVQERSMVAAKDAAVVSFLQKITEQNPNVVIPHMSAMQLLQQQQQNHPQVPAPTPAPVPPPPQPTPPMQQQQQQQTPQIAVVPVSAPAPAPVPPASVSAPVPVPVPAPAQPLPVVKNLDNGGGGENMLQPSPSRWPKAEINALINLRTTLDTKYQENGPKGPLWEEISLAMRKLGYNRNAKRCKEKWENINKYYKKKAARSDPRIRKPARIFTSLTLFTVKEQAVVKPETQMAPIMARPEQQWPLPAVVQEQQQHQQQQQKQQLKLNEPRCVQDHQNEDEYDDEDEDDDEEEEGGDYEVVPNKTSSMAAVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G976</accession>
    <name>A0AAD5G976_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF676 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_016848</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010339">
        <property type="protein sequence ID" value="KAI7732011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G976"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000188">
        <property type="entry name" value="putative lipase ROG1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007751">
        <property type="entry name" value="DUF676_lipase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044294">
        <property type="entry name" value="Lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12482">
        <property type="entry name" value="LIPASE ROG1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12482:SF14">
        <property type="entry name" value="LIPASE YOR059C ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05057">
        <property type="entry name" value="DUF676"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF676" evidence="1">
        <location>
            <begin position="62"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7732011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="392" mass="44560" checksum="CB1054F557F3D7C5" modified="2024-05-29" version="1" fragment="single">KIQKVTIYVDNKTASVKPFRHLYVFFFYDHSQCMTENGHVCSSETVNGGQNVFSCNESDVSSADHLVVMVNGILGSSADWKFAAEQFVKSLPDKVFVHRSERNAASQTLDGVDVMGERLSEEVLEVIKQKPNLRKISFVAHSVGGLVARYAIGRLYRPPQENTDNLSVENCEEAKPTIGGLIPVNFITVATPHLGSRGNKQVPFLFGLPALEKAAVLVIHWIFRRTGRHLFLTDNDEGKPPLLKRMLEDYDDCYFMSALRSFHRRVTYANVGYDHIVGWRTSSIRRNIELPKWQDSVDEKYPHVVYEERCKAFDFEPDSTNDDNTDDVEEELVTGLSRVSWEKVDVSFHNSRSRFAAHSVIQDLSVIPLSQPNGRVKKSNEFWRIGMDQRSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G9P0</accession>
    <name>A0AAD5G9P0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Histone H2B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">M8C21_024187</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">AA19_3_7</strain>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the histone H2B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010205">
        <property type="protein sequence ID" value="KAI7732581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G9P0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22910">
        <property type="entry name" value="HFD_H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000014">
        <property type="entry name" value="Histone H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000558">
        <property type="entry name" value="Histone_H2B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055333">
        <property type="entry name" value="HISTONE_H2B_site"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23428">
        <property type="entry name" value="HISTONE H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00621">
        <property type="entry name" value="HISTONEH2B"/>
    </dbReference>
    <dbReference type="SMART" id="SM00427">
        <property type="entry name" value="H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00357">
        <property type="entry name" value="HISTONE_H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5 12" id="KW-0158">Chromosome</keyword>
    <keyword evidence="9 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="11 12" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="10 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="14">
        <location>
            <begin position="47"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAI7732581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="148" mass="16250" checksum="B5BD38DD60E64D10" modified="2024-05-29" version="1">MAPKAEKKPAEKKPAEDKKTPAAEKAPAEKKPKAGKKLPKEAGAGSTDKKKKRTKKSVETYKIYIFKVLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSRLARYNKKPTITSREIQTAVRLVLPGELAKHAVSEGTKAVTKFTSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GA09</accession>
    <name>A0AAD5GA09_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_025473</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009633">
        <property type="protein sequence ID" value="KAI7734845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GA09"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7734845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="226" mass="24985" checksum="D8DA204A7D5D172B" modified="2024-05-29" version="1">MKMNVEKLEKMAAASVRTGGNGSVRIKKNNKKRDDDITSPLKPTTGGSSNPSVVVQQQLRKSPFALSSSSDDDDDDAAASAEDIHASSDDDDPFFAIRAKNYIRKAHLLGYPHDGRSVSDVRREWAEIHRKQPSCLDQLLSVERMLAGTEDPDDPDPQLARITQAINKIPYARNHIVKAYYSVCWEFFDSILCHMNFSNFGSIAGGTDVPMAHSFPSLFALEATKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GBE5</accession>
    <name>A0AAD5GBE5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein ABIL2-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_014155</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Involved in regulation of actin and microtubule organization. Part of a WAVE complex that activates the Arp2/3 complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009431">
        <property type="protein sequence ID" value="KAI7735852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GBE5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028457">
        <property type="entry name" value="ABI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10460:SF56">
        <property type="entry name" value="ABI FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10460">
        <property type="entry name" value="ABL INTERACTOR FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="186"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="235"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="199"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="241"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="254"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="271"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="289"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00025223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7735852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="332" mass="37152" checksum="66BAB48D9DA63CCD" modified="2024-05-29" version="1">METLTSTAALAEPANYDEIAMQQSLLFSDSLKAISSSSSSLGPIKQLYAAAEYFELSYTNDDQKQLVVDTLKDYAIKALVNTVDHLGAVSSKVNDLLGEKVNEVSQTEHRVSCIQQRLGICQGCFDHEGLAEQSSLINIPKYHKRYILPVGETMQGGIRTKSKYQGCSLDDEDDWHEFKNAVRATIRETPTSSFRKGRSLSPSPRGSQQPGSFAFTGAVTRKDLEKRSVSPRRFSLLRIGSLSSRPTTPNSRANSRPTTPNSRRPTTPNSIIQQRFSESPKSASMRVHSARDRYKESDQNQIPSKSKRLLKALLSRRKSKKDDTLYTYLDEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GCQ2</accession>
    <name>A0AAD5GCQ2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ATP-dependent RNA helicase</fullName>
            <ecNumber evidence="8">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_012683</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">RNA helicase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="domain">
        <text evidence="8">The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DEAD box helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="8"/>
    <dbReference type="EMBL" id="JAMZMK010009464">
        <property type="protein sequence ID" value="KAI7735581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GCQ2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd17949">
        <property type="entry name" value="DEADc_DDX31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025313">
        <property type="entry name" value="SPB4-like_CTE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24031">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13959">
        <property type="entry name" value="CTE_SPB4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01178">
        <property type="entry name" value="DUF4217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7" id="KW-0347">Helicase</keyword>
    <keyword evidence="2 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="12">
        <location>
            <begin position="13"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="45"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="11">
        <location>
            <begin position="298"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="544"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="6">
        <location>
            <begin position="13"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="548"/>
            <end position="572"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU365068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7735581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="579" mass="65870" checksum="905BC221199ECA71" modified="2024-05-29" version="1">MAEKKEIEIFASCDFASLGLHPALCDQLRDRMGFEAPTLVQAQAIPLILSGRHVLVNAATGTGKTVTYLAPVIHQLQNYPHRIQRSHGTFALVIVPTHELCMQVYENLHKLLHRFHWIVPGYIMGGENRSKEKARLRKGISILIATPGRLLDHLKHTSSFVYKNLRWIIFDEADRILELGFGKEIEEILDILGSRTQMSESKDAEFVRQNLLLSATLNEKVNHLANISLDNPVMIGLDDTKIQPSNMQVTLLDKAIETLENSGKVSTPSNAEYKLPAQLNQRYVKVPCGSRLVVLLSVLKNLFTREPSQKIVVFFSTCDAVDFHHALISQFHGLSKLQSDIETRELFVKCNILRLHGNMKHEDRVKTFNAFKTEKSALLLSTDVSARGLDFPKVRCIIQYDSPGEATEYVHRVGRTARLGEKGESLLFLQPIEADYLEELKKHGVMLTEYPLMKLLDGFPLKQQRMKKFVSIEMHPWVLDLQKSLESFISSEGKIKKVAQKAFCSWVRAYSAHRGELKSIFMVKKLHLGHVAKSFALKEQPSLVGRSVQKKHTEKRKRTHKVKEGLKKKRKMVNYEEGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GCW0</accession>
    <name>A0AAD5GCW0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">K-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_017056</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008982">
        <property type="protein sequence ID" value="KAI7737622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GCW0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050142">
        <property type="entry name" value="MADS-box/MEF2_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002487">
        <property type="entry name" value="TF_Kbox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48019">
        <property type="entry name" value="SERUM RESPONSE FACTOR HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01486">
        <property type="entry name" value="K-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51297">
        <property type="entry name" value="K_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="K-box" evidence="1">
        <location>
            <begin position="31"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7737622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="162" mass="18455" checksum="468D95AE98B9E507" modified="2024-05-29" version="1">MLTCHSMESILERYERYCHSETQLVAADATPRSWTLEYNKLKSRAELLQRNHRHYMGEDIESLSLKEIQNLEQQLDTGLKNIRARKNQLLHESISSLQKKVAIFWKGDTGAEHYFDKASEGNYNQAASSTAADGGEGADGTTNQLDLSLQPIYSCHLRHFPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GD89</accession>
    <name>A0AAD5GD89_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_022977</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009073">
        <property type="protein sequence ID" value="KAI7737244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GD89"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7737244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="79" mass="9363" checksum="F5A28E46EBA58BE5" modified="2024-05-29" version="1">MKHLNKKIPEIAAKRSQMYAYLLEEEKSLEVLPELPEFELSLKAEEYEMADKDGAWDRANWKIPNQTLKRHLTDDDEDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GDB5</accession>
    <name>A0AAD5GDB5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_018061</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008880">
        <property type="protein sequence ID" value="KAI7738072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GDB5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7738072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="201" mass="22763" checksum="FB11206EFA1430C9" modified="2024-05-29" version="1" fragment="single">MQEGRLASIVPQGAPVPVNVRFIRKWQPRGRKSNTYYFLIDKHGDGIQASVHWMDKDMYDKKITLANCYMLDKYICDDQPSAPRMSPHPASIRLGTNTDINPIPTPIDFPTNCFYLCPYADLHLHTDPNYPLHTDFMAKLEGSAPDKTCQGRDFFGEKITATLWEEALPFCNVGDMYAAEEPIIVAVTSLRVGKFYGKTLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GDY8</accession>
    <name>A0AAD5GDY8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Erythronate-4-phosphate dehydrogenase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_032347</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009168">
        <property type="protein sequence ID" value="KAI7736916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GDY8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37244">
        <property type="entry name" value="NADP-SPECIFIC GLUTAMATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37244:SF1">
        <property type="entry name" value="NADP-SPECIFIC GLUTAMATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="226"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7736916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="256" mass="29019" checksum="16FF328FEC2B8DF3" modified="2024-05-29" version="1">MGLHLLEKTMDTPTHLENGFNGPTHKSYYQPGPKSSLAWLDLRVFYIRISKCDINNESTPEYLTLNHVPLNRDTLLEVNGARTSIYSDGVTTLLRRDRIDKKSEEVTFVSTESVRTTGSVKFEVFDKDALLVSGVFYLGHSNGVHGESNNCFVSKWRMECETDMVVGHGFLKGKHNMGIELDPPSIEDQDYSHYKPEHEHHNHYPRMEYYENEDGELSWFNAGVRVGVGIGLSVCLGVGIGVGLLARTYHRTRRLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GE79</accession>
    <name>A0AAD5GE79_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_009010</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009096">
        <property type="protein sequence ID" value="KAI7737171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GE79"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:004688">
        <property type="entry name" value="Cell division control protein 48 homolog D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.760:FF:000013">
        <property type="entry name" value="Probable inactive ATP-dependent zinc metalloprotease FTSHI 4, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.760">
        <property type="entry name" value="Peptidase M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037219">
        <property type="entry name" value="Peptidase_M41-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23076:SF99">
        <property type="entry name" value="INACTIVE ATP-DEPENDENT ZINC METALLOPROTEASE FTSHI 4, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23076">
        <property type="entry name" value="METALLOPROTEASE M41 FTSH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140990">
        <property type="entry name" value="FtsH protease domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0809">Transit peptide</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042248637" description="AAA+ ATPase domain-containing protein" evidence="3">
        <location>
            <begin position="16"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="4">
        <location>
            <begin position="182"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7737171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="697" mass="78263" checksum="F45FA6B105EA3A19" modified="2024-05-29" version="1">MISNLILLVIPKTLSLFAVILPYYKDGESKKEVVFRRHIVDRMPVDSWNEVWQKLHQQVVNVDVVNLNTEVYSSIATIVVWSMRLALAIGLYVWIDNIMRPIYAKLIPCDLGSPPKKIRRQPIKNQALGSLGESRAKFISAEETTGVSFDDFAGQEYIKRELQEIVRILKNEEEFQNKGIYCPKGVLLHGPPGTGKTLLAKAIAGEAGLPFFAANGTDFVEEAKLAGRTKLHHSLAQINTRILTIKSTPRMFVGVAASRVKDLFASSRSFAPSIIFIDEIDAIGSKRGGPDIGGGGAEREQGLLQILTEMDGFKESTSQVLVIGATNRLDILDPALLRKGRFDKIIRVGLPSKDGRFAILKVHAKNKFFHSNNEMNRNEAGILTARKDMDYIGREELLEALKRQKGTFETGQEDKSDVPEELKLRLAYREAAVSVLACCFPDPHRPFQGTDINSVKSQPNMRYLEISGRVFKRKSDYVNSIIRACAPRVIEEEMFGLDNLCWISSKATLEASMLAEFLILQSGMTSFGKAYYRKQRDLVPNLAAKLEALKDEYMRFAMEKCYSVLREYYSAVEEITDILLEKGEIEADEIWSIYKSFPRISQPSVTPVDEYGALIYAGRWGIHGASLPGRATFAPGNVGFVTFGAPRPMETQVVSDETWKLVDKIWDTRVQEIRDGAKREIEAEKEKPRVLIASHFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GF27</accession>
    <name>A0AAD5GF27_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein LUTEIN DEFICIENT 5, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_025768</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008531">
        <property type="protein sequence ID" value="KAI7740020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GF27"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010291">
        <property type="term" value="F:beta-carotene 3-hydroxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016123">
        <property type="term" value="P:xanthophyll biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11046">
        <property type="entry name" value="CYP97"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050196">
        <property type="entry name" value="Cytochrome_P450_Monoox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291:SF171">
        <property type="entry name" value="PROTEIN LUTEIN DEFICIENT 5, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0349">Heme</keyword>
    <keyword evidence="3 4" id="KW-0408">Iron</keyword>
    <keyword evidence="3 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="2 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="50"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="52"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="3">
        <location>
            <position position="526"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7740020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="607" mass="67676" checksum="8A43A560D8DE809F" modified="2024-05-29" version="1">MVASLTTLQFPSPYLQSHITPTTKFNLKSPLIPKFNGVSRSCGIKCSYSNGRKPDSGEGKSEMTKEEKRRAELSARIASGAFTVEKPSLGSLLVSSLAKLGMPSAFLEPLSNLINAGENYPKIPEAKGAISAIRSEAFFIPLYELFLTYGGIFRLTFGPKSFLIVSDPIIAKHILKDNAKAYSKGILAEILEFVMGTGLIPADGEVWRVRRRVIVPALHLKYVAAMIGLFGEATDRLCKKLDAAAYNGEDVEMESLFSRLTLDIIGKSVFNYDFDSLTTDNGIVEAVYTVLREAEDRSVSPIPTWEIPIWKDISPRQKKVNEALKLINTTLDDLIAICKRMVDEEDVQFNEEYMNESDPSILHFLLASGDNVSSKQLRDDLMTMLIAGHETTAAVLTWTFYLLSKEPEIMSKLQNEVDAVLGDRFPTMEDMKKLKYTTRVINESLRLYPQPPVLIRRSIDDDVLDKYPIKRGEDLFISVWNLHRSPTHWVDADKFNPERWPLDGPNPNESNQNFSYLPFGGGPRKCVGDMFASFEAIVAVAMLVRRFNFQMALGAPPVKMTTGATIHTTEGLNMTVTRRMKPPIVPTLETQNLGSSVNITEADPVVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GF42</accession>
    <name>A0AAD5GF42_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Expansin-like EG45 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_010503</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008884">
        <property type="protein sequence ID" value="KAI7738041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GF42"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009627">
        <property type="term" value="P:systemic acquired resistance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22269">
        <property type="entry name" value="DPBB_EG45-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.10:FF:000005">
        <property type="entry name" value="Barwin-related endoglucanase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="entry name" value="RlpA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044206">
        <property type="entry name" value="EGC1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007112">
        <property type="entry name" value="Expansin/allergen_DPBB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009009">
        <property type="entry name" value="RlpA-like_DPBB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47295:SF10">
        <property type="entry name" value="EG45-LIKE DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47295">
        <property type="entry name" value="EG45-LIKE DOMAIN CONTAINING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03330">
        <property type="entry name" value="DPBB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00837">
        <property type="entry name" value="DPBB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="Barwin-like endoglucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50842">
        <property type="entry name" value="EXPANSIN_EG45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042057090" description="Expansin-like EG45 domain-containing protein" evidence="4">
        <location>
            <begin position="24"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="Expansin-like EG45" evidence="5">
        <location>
            <begin position="26"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7738041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="129" mass="13706" checksum="0E59657AE8160C7C" modified="2024-05-29" version="1">MGLMTRAFILIAMVACFTSVAHAIAGQATFYTPPYVPSSCYGFEDRGNMILAANSGLFNNRAACGQRYRVTCTSGTNAVPNPCTGRSVDVTVVDLCPGCAGNQVDLSQEAFQVIANPDAGRVNIDYNRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GF81</accession>
    <name>A0AAD5GF81_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Carboxypeptidase</fullName>
            <ecNumber evidence="10">3.4.16.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_001293</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the peptidase S10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.-" evidence="10"/>
    <dbReference type="EMBL" id="JAMZMK010008485">
        <property type="protein sequence ID" value="KAI7740217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GF81"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11320:FF:000002">
        <property type="entry name" value="Carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000013">
        <property type="entry name" value="Carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11320">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.940">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033124">
        <property type="entry name" value="Ser_caboxypep_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018202">
        <property type="entry name" value="Ser_caboxypep_ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF32">
        <property type="entry name" value="SERINE CARBOXYPEPTIDASE-LIKE 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00560">
        <property type="entry name" value="CARBOXYPEPT_SER_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00131">
        <property type="entry name" value="CARBOXYPEPT_SER_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 10" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041769597" description="Carboxypeptidase" evidence="10">
        <location>
            <begin position="22"/>
            <end position="880"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7740217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="880" mass="98124" checksum="E11BF5F0CC492C0F" modified="2024-05-29" version="1">MSHLNLGFIISILISLNPILSLSYTDPLTQQKLDQVTNLPGQTFNVNFAHYAGYVTVNQENGRALFYWLTEATEDPASKPLVLWLSGGPGCSSIGLGMAEEMGPFHVNKDGKSVYFNPYSWNTEANLLFVDSPVGVGYSYSNTSSDIWSNGDKRTGESYAGHYVPQLSQAIVRYNKANTGSPINIKGYMVGNALTDDYHDQVGLFQFMWGVGMISDQTYKKLNELCVKESLMYTSSECKQITDIANEEMGNIDINSIFTPTCTGSVVTKRLLRKWHRVGHIGQSYDPCTKQYSTAYFNLPEVQNALHAFHFNSSNAWETCSNLTYWLDAPTSVLDVYQELISSGLRIWMFSGDTDAEIPTAATRYSINALNLTTISPWRAWWEDGHKIRGAGHEVPLHKPKQALSLLKSFLAGTSMAPFEQVIESKTDSASQQLDQVTNLPGQTFNVDFTHYAGYVTVNQENGRALFYWLTEATEDPASKPLVLWLNGGPGCSSIAFGMAEEVGPFHVNKDGKSVYFNPYSWNTVANLLFLDSPAGVGYSYSNTSSDIRSNGDKRTAEDALQFLLNWLERFPEYKGRDFYLTGESYAGHYVPQLSQAIVRYNKANAGSPINIKGYMVGNALTDDYSDHVGLFQFMWAAGMISDQTYKKSNELCDKESFIHPSPECEQITDIAYDEMGDIDPYSIFTPTCTGSGIKKRLLRKWRRVGHIGQSYDPCTEQHSIAYFNLPEVQNALHAFHFNSSNTWVTCSGVVGMYWQDSPISVLDVYQELISSGLRIWMFSGDTDAVIPTASTRYSIDALNLTTISPWRAWYEDGQVGGWTQKYEGLTFVTVRGAGHEVPLHRPKQALSLLKSFLAGTPMAQFEPVIESKTDSASQDVNSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GH10</accession>
    <name>A0AAD5GH10_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Adaptor protein ClpS core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_006875</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008312">
        <property type="protein sequence ID" value="KAI7740964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GH10"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1390.10:FF:000006">
        <property type="entry name" value="ATP-dependent Clp protease adapter protein CLPS1, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022935">
        <property type="entry name" value="ClpS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003769">
        <property type="entry name" value="ClpS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014719">
        <property type="entry name" value="Ribosomal_bL12_C/ClpS-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33473">
        <property type="entry name" value="ATP-DEPENDENT CLP PROTEASE ADAPTER PROTEIN CLPS1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33473:SF17">
        <property type="entry name" value="ATP-DEPENDENT CLP PROTEASE ADAPTER PROTEIN CLPS1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02617">
        <property type="entry name" value="ClpS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54736">
        <property type="entry name" value="ClpS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Adaptor protein ClpS core" evidence="2">
        <location>
            <begin position="78"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7740964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="158" mass="17498" checksum="511AB292525A3E15" modified="2024-05-29" version="1">METTISGRLAPSPNRHLLHSNSGDKYLLHKQSTKWITVMTMAPPRMEKGGGVLDKPVIEKTTPGRESEFDLKKSKKMSPPYRVMLHNDNYNKREYVVQVLMKVIPGMTVDNAVNIMQEAHINGLSVVIVCGQPDAEEHCTQLRGNGLLSSIEPDSGGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GJI4</accession>
    <name>A0AAD5GJI4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Rhamnogalacturonase A/B/Epimerase-like pectate lyase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_007627</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 28 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007977">
        <property type="protein sequence ID" value="KAI7742466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GJI4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004650">
        <property type="term" value="F:polygalacturonase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051801">
        <property type="entry name" value="GH28_Enzymes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000743">
        <property type="entry name" value="Glyco_hydro_28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024535">
        <property type="entry name" value="RHGA/B-epi-like_pectate_lyase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31339:SF77">
        <property type="entry name" value="GLYCOSIDE HYDROLASE, FAMILY 28, PECTIN LYASE_VIRULENCE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31339">
        <property type="entry name" value="PECTIN LYASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00295">
        <property type="entry name" value="Glyco_hydro_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12708">
        <property type="entry name" value="Pect-lyase_RHGA_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00710">
        <property type="entry name" value="PbH1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041984581" description="Rhamnogalacturonase A/B/Epimerase-like pectate lyase domain-containing protein" evidence="5">
        <location>
            <begin position="28"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="domain" description="Rhamnogalacturonase A/B/Epimerase-like pectate lyase" evidence="6">
        <location>
            <begin position="45"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7742466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="447" mass="49179" checksum="CBB566AC17663B07" modified="2024-05-29" version="1">MRRGCVWEMLACVTTALLVLNCCMVAADVATCSGIVPMVYRYDKISITDFGGVGDGRTLNTKAFEEAIYRIKHLRRRGGTILYVPSGVYLTESFNLTSRMTLYLAKGAVIKATQKTGNWPLIAPLPSYGRGRERPGGRYISFIHGEGLHDVIITGEKGTIDGQGGVWWNMWRRRTLRYTRPNLVEFKDSKGIIISNVIFKNSPFWNIHPVYCSHVVIRHVTILAPPSSPNTDGIDPDSSSHVCIEDSYISTGDDLVAIKSGWDEYGIKYGRPSTDITIRRVVGSSPFAGIAVGSETSGGIYNVLAEHITLKNMGVGIHLKTNIGRGGIIKNITISDVYMVNVRKGIKIAADVGDHPDKNYNPNALPVLNHVRIKNVRGKKVEQAGLIQGLKNSPFTDICLSNINLHGYPRRNNVPWKCSYVSGGAIQVRPSPCSDLIHRSSCPIQGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GKJ5</accession>
    <name>A0AAD5GKJ5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_014138</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006995">
        <property type="protein sequence ID" value="KAI7746322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GKJ5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044809">
        <property type="entry name" value="AUF1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31215">
        <property type="entry name" value="OS05G0510400 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7746322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="206" mass="23600" checksum="0DD01E41454F5022" modified="2024-05-29" version="1">MDKLPGSLLLEILSRLDDSADVACCRVASKAFDDVSPFLRSINIGCSSKWFWDSSSPFKRIFLDLISKQETVESVSISRYSGYLYDVDFAKEWLPRVSKSLKSLSLSSHNLVNLKLRSARLSMHNLNPLPMLTSLTLEYTELEDEQLNGFNKCFPNLQHLYLIAVVGHQNPKIHHLNLQACHLDFYRYPPPHLTQNRKCLSCRHSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GKN2</accession>
    <name>A0AAD5GKN2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Bulb-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_014962</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007699">
        <property type="protein sequence ID" value="KAI7743696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001480">
        <property type="entry name" value="Bulb-type_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036426">
        <property type="entry name" value="Bulb-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32444">
        <property type="entry name" value="BULB-TYPE LECTIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32444:SF132">
        <property type="entry name" value="S-LOCUS-SPECIFIC GLYCOPROTEIN_EP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01453">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51110">
        <property type="entry name" value="alpha-D-mannose-specific plant lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50927">
        <property type="entry name" value="BULB_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042275135" description="Bulb-type lectin domain-containing protein" evidence="3">
        <location>
            <begin position="24"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="Bulb-type lectin" evidence="4">
        <location>
            <begin position="46"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7743696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="263" mass="29886" checksum="99F5878CC33A215C" modified="2024-05-29" version="1">MAAYSSFLSFIFISLLFSSLSISNGIVPANETFRYVNEGELRYSNDYMIYLSEYAPTYRPLPAFTFPFQLCFYNTTPNAYTLGLRMGTVRSQTVMAWVWEANRGKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGFAILSDGNMVLRDSKGKFVWQSFDSPTDTLLVGQTLRVRGGPYKLVSRESVTNNVDGVYSLVIEPKRLALYYKNMPYWSSTFPELDRKNVAIVDATLIWDKFGTIAHYHISDWASMGTYDSTLTVQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GMK9</accession>
    <name>A0AAD5GMK9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ras-related protein RABA4d</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_008159</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Endomembrane system</location>
            <topology evidence="7">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006529">
        <property type="protein sequence ID" value="KAI7748405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GMK9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01868">
        <property type="entry name" value="Rab11_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000274">
        <property type="entry name" value="ras-related protein RABA5a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0636">Prenylation</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7748405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="213" mass="23548" checksum="D87DF2A4C66C2FBA" modified="2024-05-29" version="1">MSNNNEKVDYVFKVVLIGDSSVGKTQLLARFTKNEFRVDSKATIGVEFQTQTLVIDQKTVKAQIWDTAGQERYRAVTSAYYRGALGAMLVYDMTKRQTFEHMTRWLEELRGHADKNIVIMLIGNKCDLASLRAVPVEDAQEFAERENLCFMETSALESTNVESAFLTALTEIYKITCKKSLSADGGYGKSSLKGTAILVPNQDADSGGKGGCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GNK7</accession>
    <name>A0AAD5GNK7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Polynucleotide phosphorylase 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_000428</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007014">
        <property type="protein sequence ID" value="KAI7746263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GNK7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009570">
        <property type="term" value="C:chloroplast stroma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004654">
        <property type="term" value="F:polyribonucleotide nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000958">
        <property type="term" value="P:mitochondrial mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000965">
        <property type="term" value="P:mitochondrial RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02393">
        <property type="entry name" value="KH-I_PNPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000001">
        <property type="entry name" value="Polyribonucleotide nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="entry name" value="GHMP Kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001247">
        <property type="entry name" value="ExoRNase_PH_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036345">
        <property type="entry name" value="ExoRNase_PH_dom2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012162">
        <property type="entry name" value="PNPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11252">
        <property type="entry name" value="POLYRIBONUCLEOTIDE NUCLEOTIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11252:SF0">
        <property type="entry name" value="POLYRIBONUCLEOTIDE NUCLEOTIDYLTRANSFERASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01138">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55666">
        <property type="entry name" value="Ribonuclease PH domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="K Homology" evidence="6">
        <location>
            <begin position="179"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00031451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7746263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="275" mass="29174" checksum="47564D9E07670049" modified="2024-05-29" version="1" fragment="single">YSFPSSCVGEVGRIGAPSRREIGHGTLAERALEPILPSEDEFPYTIRVESTITESNGSSSMASVCGGCLALQDAGVPLKSSIAGIAMGMVLDTKEFGGDGTPLILSDITGSEDASGDMDFKVAGSDDGVTAFQMDIKVAGITLSTMKHALLQAKEGRKRILAEMLKCSPPPSKQLSKYAPFILVMKVKPEKVNKIIGSGGKKVKSIIEETGVESIETQDNGTVKIMSRDLASLEKTKAIISNLTMVPIVGDVYRNCKITSIVPYGVFVEIVPGRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GNN2</accession>
    <name>A0AAD5GNN2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_017523</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="JAMZMK010006712">
        <property type="protein sequence ID" value="KAI7747654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GNN2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13983">
        <property type="entry name" value="STKc_WNK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000075">
        <property type="entry name" value="Probable serine/threonine-protein kinase WNK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000046">
        <property type="entry name" value="probable serine/threonine-protein kinase WNK9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050588">
        <property type="entry name" value="WNK_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13902">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE WNK WITH NO LYSINE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="36"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7747654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="488" mass="55441" checksum="DD35EA9C36DDBB56" modified="2024-05-29" version="1">MGSCGNNNNGLSGGGDGGVAALEVDYVEMDPSGRFIRYPEVLGKGTFKKVYKAFDQFEGVEVAWNRIRIDNVLQSPDEYKKLYSEVHLLKSLKHRNIIKLYDYWIDVKKKTLNMITELFTSGNLRVYRKKHRGVDMKAIKNWARQILNGLDYLHSQNPPVIHRDLKCDNLFVNGNHGEIKIGDLGLATVLQQPTARSFIGTPEFMAPELYEEEYNELVDIYSFGMCMLEMATFEYPYSECKNPAQIYKKVTSGIKPASLDKVSDPEIKEFIEKCLVPANQRLSAKELLEDSFLKPKNPKQPTQDPVAKLNPSVVELCRIHQDNEFRLKGTKNNSNSVSLTMRIADPSGRVKNIHFLFYLATDTALSVAREMVEQLELAKNNVPFIADMIKNSIARILPSRQPLNKNKPEFTRVTSMVEHGFGNLSRFSNVEALTTVGSYLSLCESDDKPNVMTQLEAVERGYKHWCKEVKLVKKKKRVEATLKRSLTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GNS4</accession>
    <name>A0AAD5GNS4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">WAT1-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_011751</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the drug/metabolite transporter (DMT) superfamily. Plant drug/metabolite exporter (P-DME) (TC 2.A.7.4) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006861">
        <property type="protein sequence ID" value="KAI7746996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GNS4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030184">
        <property type="entry name" value="WAT1-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31218">
        <property type="entry name" value="WAT1-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="61"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="88"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="126"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="171"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="203"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="242"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="267"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="293"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="7">
        <location>
            <begin position="7"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="7">
        <location>
            <begin position="174"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7746996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="346" mass="37351" checksum="16E862EDD5760489" modified="2024-05-29" version="1">MKEYVGMVAAQTAQVLLMIVSKSAIADGMSNYSFVFYSNALASLVLLPLSLIFHRSVNRPSLSLIVVGGFIVIGLLGFMAQITGYTGISYSSATLATTLLNLIPGFTFILAIICRMEIIQFGSSATQAKFIGTVVSVAGAIMVTLYKGPPILSSPLKSEILKNLTAQPSNWVLGGIFLAIDAVFASMYIIAQAFVLKKYPAEMIVMNAYCVVCTILSGLTSLVVEDDLSSYSLQPKKRLLSILYSGIFGSAFQVTVQAWCVRRKGPFFVAMFHPVGIVISTFIGVIFLGDGFYLGSLMGSIIVVIGFYSVMWGKAKEQKIVAVSKTEDEHAPLLQDVEEQMTRSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GQ83</accession>
    <name>A0AAD5GQ83_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 9">Phenylalanine ammonia-lyase</fullName>
            <ecNumber evidence="5 9">4.3.1.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_023444</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This is a key enzyme of plant metabolism catalyzing the first reaction in the biosynthesis from L-phenylalanine of a wide variety of natural products based on the phenylpropane skeleton.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-phenylalanine = (E)-cinnamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:21384"/>
            <dbReference type="ChEBI" id="CHEBI:15669"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="EC" id="4.3.1.24"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="9">Phenylpropanoid metabolism; trans-cinnamate biosynthesis; trans-cinnamate from L-phenylalanine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the PAL/histidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.3.1.24" evidence="5 9"/>
    <dbReference type="EMBL" id="JAMZMK010005931">
        <property type="protein sequence ID" value="KAI7751347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GQ83"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045548">
        <property type="term" value="F:phenylalanine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006559">
        <property type="term" value="P:L-phenylalanine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009698">
        <property type="term" value="P:phenylpropanoid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00332">
        <property type="entry name" value="PAL-HAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.200.10">
        <property type="entry name" value="Fumarase/aspartase (Central domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="entry name" value="Fumarase/aspartase (N-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001106">
        <property type="entry name" value="Aromatic_Lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022313">
        <property type="entry name" value="Phe/His_NH3-lyase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005922">
        <property type="entry name" value="Phe_NH3-lyase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01226">
        <property type="entry name" value="phe_am_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10362">
        <property type="entry name" value="HISTIDINE AMMONIA-LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00221">
        <property type="entry name" value="Lyase_aromatic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="L-aspartase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00488">
        <property type="entry name" value="PAL_HISTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-0585">Phenylalanine catabolism</keyword>
    <keyword evidence="9" id="KW-0587">Phenylpropanoid metabolism</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7751347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="529" mass="57780" checksum="B8C1052FEAF92565" modified="2024-05-29" version="1" fragment="single">YGVTTGFGATSHRRTKNGGALQKELIRFLNAGIFGNGTESSHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAITKLLNNNITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAVGPDGEILNAEQAFTKAGVEGGFFELQPKEGLALVNGTAVGSGMASMVLFEANVLALLSEVLSAIFAEVMQGKPEFTDHLTHKLKHHPGQIEAAAIMEYILDGSDYVKAAKKVHEMDPLQKPKQDRYALRTSPQWLGPQIEVIRSATKMIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIAAIGKLMFAQFSELVNDFYNNGLPSNLSGGRNPSLDYGFKGGEIAMASYCSELQFLANPLHPSRFCEKDLLRVVDREYVFAYIDDPCLATYPLMQKLRQVLVDHALNNGETEKNVNTSIFQKIAAFEDELKAILPKEVEGVRTAFENGTLSIPNRIKACRSYPLYRFVREELGGVYLTGEKVTSPGEEFDKVFTAMSKGQIIDPLLECVEGWNGAPLPIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GQP1</accession>
    <name>A0AAD5GQP1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase S9 prolyl oligopeptidase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_007611</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006526">
        <property type="protein sequence ID" value="KAI7748453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GQP1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42776:SF28">
        <property type="entry name" value="GLUTAMYL ENDOPEPTIDASE, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42776">
        <property type="entry name" value="SERINE PEPTIDASE S9 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="3">
        <location>
            <begin position="601"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="73"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="73"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7748453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="705" mass="78373" checksum="B2CCACF3B4982E59" modified="2024-05-29" version="1">MQLHKLSLTSPTLLLRLPPSSTLFSSVHLKRCRISSSKTRNHSDNSTFSFIQSISSSRLHNLVPVAALATKDAAAGPNGSSPAPQDDELNSGYRLPPPEIRDIVDAPPLPSISFSPNRDKILFLKRRSLPPLSELAKPEDKLAGIRIDGRSNTRSRMSFYTGIGIHDLKDDGTLGPEKLIHGFPDGSKLNFVTWSTDGKHLAFSIRQDQDDDDDGSKLRVWVADVETGKAKPLFHSPDVFLNAVFDNYVWVNDSTLLVCTIPSSRGSPPKKPLVPAGPKIQSNERKNVVQARTYQDLLKDEYDEDLFEYYATSQLVLVSLDGTVKLFGEPAIYTSLDPSPDQKYVLLSSIHRPFSFNVPCGRFPVKDDLWSADGKFLRTLCDLPLAEDIPITFNSVRKGMRSLNWREDKPSDLYWVETQDGGDAKVEVSPRDIVYTQAADAPEGEEPSIFFKLDLRYGSSEDVYSDPGSPMLRRTPAGTYIIAKFKKEDREGTYLLLNGSGATPEGNIPFLDLFDVDTGHKERIWQSDKEKYYESVVALMSDQTAGDLNVNEFAILSGPTIPIIGEGKEEANDSYVEQLVWSAEAAVQEVIRRGTQESNSEDRTLWEATDTYIKMSPFMSANKIKKPILLIHGEEDNNSGTLTMQSDRFFNALKGHGALSRLVILPFESHDSLSGATIGHWLFEDTVWQTYTDAISAPENTQSIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GRE9</accession>
    <name>A0AAD5GRE9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">V-type proton ATPase subunit a</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_018337</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Essential component of the vacuolar proton pump (V-ATPase), a multimeric enzyme that catalyzes the translocation of protons across the membranes. Required for assembly and activity of the V-ATPase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the V-ATPase 116 kDa subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006284">
        <property type="protein sequence ID" value="KAI7749756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GRE9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033179">
        <property type="term" value="C:proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016471">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051117">
        <property type="term" value="F:ATPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002490">
        <property type="entry name" value="V-ATPase_116kDa_su"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629:SF63">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629">
        <property type="entry name" value="VACUOLAR PROTON ATPASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01496">
        <property type="entry name" value="V_ATPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="6 8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="163"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="202"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7749756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="292" mass="33544" checksum="A66BCA52A986E0D7" modified="2024-05-29" version="1">MRVKKINDVFNSGIESLAELATEFNGHLEKAFDNINSEVVEHYRSGCFNAANPYFNILAIVVKFFCNIFHNLDIGMQEHRITVVIKRCGEMACKLHFFKDQMMKAALLASTRSSYGSDIGLDELEVKLGELESELIKMNFRTLKSYSVPIMNFWSISLSCRRLVTFSIMLKYMSSILLLPFILVAKYQEANPGLYTIVTFPFLFAVMFGDWGHDICLLLATLFLILREKKYSSQDSYNEFFSAPFELSERSAYDCRDSSCRDATTVGMIKVRDTYPFGFGMEHVVNYLFLTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GS49</accession>
    <name>A0AAD5GS49_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_022490</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CAF1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006121">
        <property type="protein sequence ID" value="KAI7750581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GS49"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051181">
        <property type="entry name" value="CAF1_poly(A)_ribonucleases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006941">
        <property type="entry name" value="RNase_CAF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15092">
        <property type="entry name" value="POLY A -SPECIFIC RIBONUCLEASE/TARGET OF EGR1, MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15092:SF42">
        <property type="entry name" value="POLY(A)-SPECIFIC RIBONUCLEASE PARN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04857">
        <property type="entry name" value="CAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7750581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="620" mass="70123" checksum="45C1FFC75CD156CA" modified="2024-05-29" version="1">MPPPFPMLQRRFLCTQTTNLSFFNPNTGRHHRPQWPIKQVTKSNFTDSLDELNTHISNSDFVAISLQNTGSYSSPWHRILPFDTTEIAYLKAKYAAERFQILQLAVCPFSVRASNKLQAHPFNFHLFQRDELKIGAPCYSFSCQPSYLASMAREDFDFNRCIYDGISYLSRAQEAAAKHQIGNPVSSTYVAQSSSSRSVADNVFVERVKSRVGHWISACKRSDMKSDDALVNSLRKLILGGDEYGSRPSMNIDVCSERQVQLVFEMVKSRYENVVPLTISAKGGGTQSVRVVLTSSKEDRDLLEEDLKNMEEEQSKRVRGFREVIELISTSQKPVVVHNSLNDFAFIHSKFISPLPPTMDEFRSSLHSVFHDIRDINQLMKEVGPLNKYSNLPSAIAYLKRNFFAPLEIDITQKASMEECKNHGHNVVRISELFARLCFILKLTLDTQHHNPDGPSPKPYANVVNLGSDGFQETDDISVWTHNPKKISINNLVFLWGFRSGISAGKLKNMLFKSHDLLSEDDTFDVRLIDKNCAVVVFWKNGLSDWFLDAIGSGDGLRELATEGLMAAGYDTYTRVCKSEILGADLADSLAEFDRVSSDHVDDSAEVYWNSELMIKFDDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GTU9</accession>
    <name>A0AAD5GTU9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">FHA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_022176</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005729">
        <property type="protein sequence ID" value="KAI7752201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GTU9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22677">
        <property type="entry name" value="FHA_Kanadaptin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.20:FF:000053">
        <property type="entry name" value="Os06g0275900 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050923">
        <property type="entry name" value="Cell_Proc_Reg/RNA_Proc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23308">
        <property type="entry name" value="NUCLEAR INHIBITOR OF PROTEIN PHOSPHATASE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FHA" evidence="3">
        <location>
            <begin position="97"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="294"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="449"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="501"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="596"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="259"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="304"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="329"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="501"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="614"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="652"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="663"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7752201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="663" mass="73657" checksum="6DAD3FDEF223CB09" modified="2024-05-29" version="1" fragment="single">FCLVRLERFAWDRFASGGNGDGDGGDGYWRRKGGLVVKKKGFVGEVQKPSSNGGGGGVTVPYTIPTWSEPPCHNYFLEVLKDGSIIDQLDVHEKGAYMFGRVELCDFILEHPTISRFHAVLQFNKSGGAFIYDLSSTHGTFINKNQVKKKVYVELHVGDVLRFGHSSRLYIFQGPSDLMPPEKDLKSIRNSKMRDERRDMEASLRRAKREAALADGISWGMDEDAVEEDEIANMKKEIDAIRAKDIAQGGLTQGQQTQIARNEQRMAQVAEELENLEETLNDSIRESLGARVGRVHGKKKGAPADDEDEYLSDEDDFYDRTKKKPSKQKGADGKSVETADSLIEKKDAISKQIEEKEKLLADEKNKAVPTNDDVAETGDELDAFMSGLSSQLVHDKTQSIQKELDELHSELERVVYLLKIADPTGEASRKRQSVKPDIPIMPVVKKEPVEKAKNVQKQATDDVMTKANKKEESSEVAVTKETEPKPVVYTVTKPQWLGAVENKETKKAEETPSVVEEPESDGFVDYKDRKEVLLNTGDLENAAPGLIIRKRKQVEEKDKVKTVNSEPVESPVGVDFAAEDAVALLLKHTRGIQAIDDEEMHSSEAAPAAEKGNNGKKKAKKAKKVLGPERPSFLDSETDYEAWVPPEGQSGDGRTSLNDRLGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GWX1</accession>
    <name>A0AAD5GWX1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_019343</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000626">
        <property type="protein sequence ID" value="KAI7756039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GWX1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7756039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="84" mass="9602" checksum="4C9E63FF82BE4C60" modified="2024-05-29" version="1">MAYNVLGLLCFSCCTDPLLLVKRAYEVVFSRRELADQLTGHVLILNLLMCGCRVIRKSFYMPYQYHGIKHWMDEVAGGICVSLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GWY2</accession>
    <name>A0AAD5GWY2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TAF6 C-terminal HEAT repeat domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_017317</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010002787">
        <property type="protein sequence ID" value="KAI7754678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GWY2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046695">
        <property type="term" value="C:SLIK (SAGA-like) complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08050">
        <property type="entry name" value="TAF6C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.770:FF:000001">
        <property type="entry name" value="Transcription initiation factor TFIID subunit 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.770">
        <property type="entry name" value="TAF6, C-terminal HEAT repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037796">
        <property type="entry name" value="TAF6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011442">
        <property type="entry name" value="TAF6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046344">
        <property type="entry name" value="TAF6_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10221">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10221:SF9">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07571">
        <property type="entry name" value="TAF6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TAF6 C-terminal HEAT repeat" evidence="7">
        <location>
            <begin position="145"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="14"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="26"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="38"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7754678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="318" mass="36660" checksum="EAEFE9D62A3377E0" modified="2024-05-29" version="1" fragment="single">MNRRRSTVVVQAVHNRVMAAPPSHNVYKTQTHILNQRSQREKGDQREREREQKEEREREKPMRESGEQTEKRGADPGHDRPALKERGTKEQRRVVVGVRRQKQRKAMEFTVVAQVGSRRLLVAALWVRKPDCKEDGGSVDVKIPVKHVLSIELQLYFEKITDLTVSRSNSVIFNKALLSLATDAGLHPLVPYFIYFVAEEVTRNLNNFQLLFALMRLVRSLLQNPYLHIEPYLHQLMPSVMTCLVAKSLGNKLTDNHWELRNFTANLVALIFRRFGYGYHNLQLRVTRTLLHCFLDPAKALSQHYGAIKAITAIGPQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BJU5</accession>
    <name>A0AAD5BJU5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">COI1 F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_005681</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012217">
        <property type="protein sequence ID" value="KAI7724460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BJU5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22159">
        <property type="entry name" value="F-box_AtTIR1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.50:FF:000023">
        <property type="entry name" value="F-box/LRR-repeat protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041567">
        <property type="entry name" value="COI1_F-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318:SF148">
        <property type="entry name" value="F-BOX PROTEIN MAX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318">
        <property type="entry name" value="PARTNER OF PAIRED, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18511">
        <property type="entry name" value="F-box_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="COI1 F-box" evidence="1">
        <location>
            <begin position="14"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7724460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="639" mass="71956" checksum="A61D9E3C2EE7FAD3" modified="2024-05-29" version="1">MSTTTTTTTTINDLPDVILFTILSSISDTRSRNSASLVSKKWLCVDRSTRSSLSLRGNARDLFLLPSCFRSISHLDLSLLSPWGHPLFSHSPTHPSLFSHLLKQSFPHLSSLIIYSRNPTPLQLLTLQWPNLNHIKLVRWHQRPPNLPPAADIIPIFENCRHIKSLDLSSFYCWTEDIPPVLKSYPMVASNLTCLNLLNPSLTEGFRGQEVEEITKYCPNLKKFFIACMFDPRYIGFVGDEAMVSISVNCPKLSVLWLADPLALVNARADLEVGFAQDDAGVTVATLIEVFSGLSMLEELTFDVSWNVRDCGPALEVLNSKCLKLKCLRIGNFHGVSMPVESKLDGLALCQRLEALSIRNVADLTDMGLIAIARGCCKLSKFEIQGCRNVTVRGMRTFVCLLSRTLIEVKISCCKNLGARASLKALEPIRDRIQKLHIDCVWDNVEDFDDIFDGVEIEDCYDDELGNKRKKQKLDFDLNDLSNGFEGQTQTWEKLEHLSLWFSVGELLTPLLSSALEDCPNLEEICIKVEGDCRHFSKPAQREFGLRCLGLYPNLSKMKLDCGEIIGYAHTAPSGQMDLSLWERFYLFGIGDLNLEELDYWPPQDRDVNQRSLSLPAAGLLQEFCVMGSRFVQYPSQPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BK64</accession>
    <name>A0AAD5BK64_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_004619</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012171">
        <property type="protein sequence ID" value="KAI7724742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BK64"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.80">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003441">
        <property type="entry name" value="NAC-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036093">
        <property type="entry name" value="NAC_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31719:SF130">
        <property type="entry name" value="NAC DOMAIN-CONTAINING PROTEIN 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31719">
        <property type="entry name" value="NAC TRANSCRIPTION FACTOR 56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02365">
        <property type="entry name" value="NAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101941">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51005">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NAC" evidence="5">
        <location>
            <begin position="6"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7724742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="190" mass="21940" checksum="8C16175CE9988D37" modified="2024-05-29" version="1">MEKMNLLPGFRFHPTDEELVVYYLMPKIQSLPLPHPIPEADVCKSDPWDLLGDSEEEKYLFSTVETKSLYDKRINRTAPSGNWKPSSTDKQVVDSRNRHVGTKKTFVFYRSKSAKGSKTEWFMHEYKLTNPIQGVQDWMICRVFKKKKGKKDAMEDEKMVQVRNTGATFYVPRNFDLNLDGDESSSSGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BKG8</accession>
    <name>A0AAD5BKG8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_014088</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10 16">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 16">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the PP2C family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="4"/>
    <dbReference type="EMBL" id="JAMZMK010012141">
        <property type="protein sequence ID" value="KAI7724905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BKG8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000008">
        <property type="entry name" value="Phosphatase 2C family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000222">
        <property type="entry name" value="PP2C_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01032">
        <property type="entry name" value="PPM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 12" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="13">
        <location>
            <begin position="46"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7724905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="389" mass="43185" checksum="C4A9F964C3A7B6ED" modified="2024-05-29" version="1">MLMNLLWACFRPRLLSERRVRNGENSGGRQEGLLWYKDTGHHVNGDFSMAVVQANNLLEDQSQVESGCLSLQDSGPYGTFVGVYDGHGGPETSRFINSNLFQHLKRFTSENQSMSVEVIKKAFEATEDGFLSLVSKQWPLNPQIAAVGSCCLVGVVCGGTLYIANLGDSRAVLGRLMKATGETVALQLSAEHNACIESVRQELHSTHPDDSQIVVLKHNVWRVKGLIQISRSIGDVYLKKAEYNRAPLYARFRLREPFKRPILSSDPSVSAHQLQPHDQFIIFASDGLWEHLTNQEAVDIVQNHPRSGSAKRLVKNALRSAAKKREMRYSDLKKIDRGVRRHFHDDITVVVLFLDSNLVSRGSSFRTPNISVKGAGVTLPSNTLAPGLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BKJ7</accession>
    <name>A0AAD5BKJ7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Trehalose-phosphatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_018876</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">In the C-terminal section; belongs to the trehalose phosphatase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">In the N-terminal section; belongs to the glycosyltransferase 20 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012115">
        <property type="protein sequence ID" value="KAI7724957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BKJ7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004805">
        <property type="term" value="F:trehalose-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005992">
        <property type="term" value="P:trehalose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01627">
        <property type="entry name" value="HAD_TPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000010">
        <property type="entry name" value="Alpha,alpha-trehalose-phosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000052">
        <property type="entry name" value="Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000054">
        <property type="entry name" value="alpha,alpha-trehalose-phosphate synthase [UDP-forming] 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1020:FF:000002">
        <property type="entry name" value="Trehalose-6-phosphate synthase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1020">
        <property type="entry name" value="Trehalose-6-phosphate phosphatase related protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001830">
        <property type="entry name" value="Glyco_trans_20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006379">
        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003337">
        <property type="entry name" value="Trehalose_PPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01484">
        <property type="entry name" value="HAD-SF-IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00685">
        <property type="entry name" value="T6PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788:SF118">
        <property type="entry name" value="ALPHA,ALPHA-TREHALOSE-PHOSPHATE SYNTHASE (UDP-FORMING), TREHALOSE-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788">
        <property type="entry name" value="TREHALOSE-6-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00982">
        <property type="entry name" value="Glyco_transf_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02358">
        <property type="entry name" value="Trehalose_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="686"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7724957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="686" mass="76524" checksum="D7C9EDE9F82063BA" modified="2024-05-29" version="1" fragment="single">ILSRLGAASLTIVRERAMSVPAVLSQSDQDHNVSSDAPSDVPNRLIVVSNQLPIIAARKTDDNGMSFWDFTWDESSIYMHIKDALPAALEVFYVGALRADVGTTEYNDVSKTLLDRFNCVASVSFLRGLQDPANEKRDLERSIEIKIKASGIHVGQMESYLSQPDTRLKIQELKKRFEGKTVLLGVDDLDIFKGVNFKVLAMEKLLKSHPGSSWLGRAVLVQILNPARGRGQDVVEINDEIRTICVRINTELGSPGYQPIVFIDWPISLSEKAAYYAVSEVAIVTPLRDGMNLIPYEYVVSRQGVDDPNANNPKKSMLVVSEFIGCSPSLTGAIRVNPWDVEDTAEAMFTALTASDLHKETVHLKHYHYITTHDVANWARSFFQDLEQTCIDHSHKRCVNLGFGLDTRVVLFDQKFSKLDTDILENAYSMAQNRAIILDYDGTITPSISKSPTEAVISLINKLCNDPKNMVFIVSGRSKENLGNWFSACEKLAIAAEHGYFIRWASDQEWEMCARENSVRWMEMAEPVMNLYTEATDGSYIEKKETAMVWHYEDADKDFGSDQAKELLDHLENVLANEPVAVKRGQDIVEVKPQGVTKGIVVEKIFAFMSEKGKQADFVLSVGDDKSDEDMFVAIADGITKGQITNNNSVFTCVVGEKPSAAEYYLDETKDVSTMLEKLGHLSNQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BLV9</accession>
    <name>A0AAD5BLV9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_031765</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011852">
        <property type="protein sequence ID" value="KAI7725808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BLV9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35506">
        <property type="entry name" value="OS02G0135600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35506:SF1">
        <property type="entry name" value="OS02G0135600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7725808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="380" mass="42832" checksum="61BADBE5A1A6585B" modified="2024-05-29" version="1">MADKPSRGLVLYGDGLARFINPSHTHLHALAAQSLCGFLSLPHLPSSENVDGRTIGEFLNLVDAYVDITTLSVDSQEKCITPSISERFMGVKAALITENKSLKSCGDKLGLSVLQPNELINKDCSLADSPVNLVAAELLNLLGFQEDKVLDSNQFDLVFVHIGGQQEDLINKYTEYINSLIGEIISKAKPKSQIGSRIHLSVVLSYGEILNDGESNFTILNRKGIMQSGFDSLYPRQSYTMKGSNPRSNVRDYCPMLFTQWQDAVTRKDTVKTISFQDFMENGGNLAIPADRFLHEVAFKLWKAPKQTNFDAPESWPYEIMWQRRWRRVTATEGRGEERKLQQPLHKGANVEESLDCYEREDWTHWLEVRSKGQEKGRSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BM23</accession>
    <name>A0AAD5BM23_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">COP9 signalosome complex subunit 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_021796</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CSN4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012145">
        <property type="protein sequence ID" value="KAI7724896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BM23"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008180">
        <property type="term" value="C:COP9 signalosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000190">
        <property type="entry name" value="COP9 signalosome complex subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054559">
        <property type="entry name" value="PSMD12-CSN4-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040134">
        <property type="entry name" value="PSMD12/CSN4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10855">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 12/COP9 SIGNALOSOME COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10855:SF2">
        <property type="entry name" value="COP9 SIGNALOSOME COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22241">
        <property type="entry name" value="PSMD12-CSN4_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0736">Signalosome</keyword>
    <feature type="domain" description="PCI" evidence="8">
        <location>
            <begin position="192"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7724896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="397" mass="45327" checksum="C195B6032F268FB9" modified="2024-05-29" version="1">MESAFANASAISDQRQKIEEYKHILSSVIASNDIVQARKFIDHILSDDVPLVVSRQLLQTFAQELGRLEPENQKEIAHYTLHQIQPRVVSFEEQVLVIREKLADLYESEQLWSKAAQMLSGIDLDSGMRVIDDKFRLTKCVQIARLYLEDDDAVNAEAFINKASFMVSNSQHEVLNLQYKVCYARILDLKRKFLEAALRYYDISQIEKRQIGDEVIDEDALEQALAAAVTCTILAAAGPQRSRVLATLYKDERCSRLKIYPILQKVYLERILRKPEIDAFSEELKAHQKALLPDNFTVLDRAMIEHNLLSASKLYTNISFDELGTLLGIEPHKAEKIASRMICEDRMRGSIDQVEAVIHFEDDNEELQQWDQQIFGLCQALNDVLDSMAKKGLAIPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BM63</accession>
    <name>A0AAD5BM63_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_011115</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012151">
        <property type="protein sequence ID" value="KAI7724823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BM63"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046474">
        <property type="term" value="P:glycerophospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000137">
        <property type="entry name" value="Hydrolase family protein / HAD-superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050324">
        <property type="entry name" value="CDP-alcohol_PTase-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006357">
        <property type="entry name" value="HAD-SF_hydro_IIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006353">
        <property type="entry name" value="HAD-SF_hydro_IIA_CECR5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01456">
        <property type="entry name" value="CECR5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01460">
        <property type="entry name" value="HAD-SF-IIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14269">
        <property type="entry name" value="CDP-DIACYLGLYCEROL--GLYCEROL-3-PHOSPHATE 3-PHOSPHATIDYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14269:SF49">
        <property type="entry name" value="HYDROLASE FAMILY PROTEIN _ HAD-SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13242">
        <property type="entry name" value="Hydrolase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7724823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="276" mass="31045" checksum="EEE4998868031E98" modified="2024-05-29" version="1" fragment="single">VVQGHSPFKNLLARFENELVVATGKGEPAVVMSEYGFKKAISLEDYASYFNSIDPLAPYKTWTTKQPCTDESKYIESVPSFDVTSERVKAAFVVSDPVDWSRDIQVLCDILRSGGLPGEKSEHQPHMYFAADDLEYQAVFPSERLGMGAFRIALESIFNSIHHKPLKYTSFGKPNPFVFKNTESILMQLQSLTSDADLNGDYENMNSRHFETLYMIGDNPSVDIKGARQAGHPWFSILTRTGVFRGKVNHTEYPADKVVDTVEEAVEFILRREGIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BME8</accession>
    <name>A0AAD5BME8_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_018877</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012115">
        <property type="protein sequence ID" value="KAI7724958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BME8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7724958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="140" mass="16200" checksum="92BA1B988ADF1E92" modified="2024-05-29" version="1" fragment="single">IEVISISTDESTASKNFHDIIVDDHHDVEIVADYEHPFVHSTVHSNPVQDLQLEKQITERFRFPMTFGKSAKFEHEQQITLHFEDQIFLNATLRAESSGKGVRFAICSWKTCMAKLGLHEGWTYRLRYNKDEGILGFYIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BMZ6</accession>
    <name>A0AAD5BMZ6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ENT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_018837</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011685">
        <property type="protein sequence ID" value="KAI7726377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BMZ6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050832">
        <property type="term" value="P:defense response to fungus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1240.40:FF:000005">
        <property type="entry name" value="ENT domain containing protein, expressed"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1240.40">
        <property type="entry name" value="ENT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033485">
        <property type="entry name" value="EMSY-LIKE_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005491">
        <property type="entry name" value="ENT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036142">
        <property type="entry name" value="ENT_dom-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33432">
        <property type="entry name" value="PROTEIN EMSY-LIKE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33432:SF28">
        <property type="entry name" value="PROTEIN EMSY-LIKE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03735">
        <property type="entry name" value="ENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01191">
        <property type="entry name" value="ENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158639">
        <property type="entry name" value="ENT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51138">
        <property type="entry name" value="ENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENT" evidence="5">
        <location>
            <begin position="52"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="124"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="277"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="357"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="124"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="154"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="277"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7726377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="390" mass="42235" checksum="717A1AD7A777AB5D" modified="2024-05-29" version="1">MDYEPYDSSGTDDDLPPSHQNRMPRGGHVAGNGRSAVMGSVQHPKMYGDTDMEAQIHQLERDAYSSVLRAFKAQADAITWEKEGLITELRKELRLSNEEHRELLLKVNSDDVIRRIREWRQSSGIQPGTVNTGQAVHDPLPSPSVSGNRKKQKMNPSGHSQSFGGPPQHVPLPTSSSAARRAPVTGPKGKSKKSMMGGLSSIKAQPSPSGATGRAQLGNRATSDYNPAPTLNIGRHALVYDMYTANETWEWVNLSEISPEDIQWVEAEDPGIPLKTGYGGPGHGIGRPVGRESGPGGGRGRGRGRGMPKGQTRKVFPPSQNGIGKKGLDDLQLYNTDTLIKEVERVFGSSHPDPLEIEKARKVLKEHEEALADAIAKLADISDGESGTIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BNA2</accession>
    <name>A0AAD5BNA2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_017401</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the WIP C2H2-type zinc-finger protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011643">
        <property type="protein sequence ID" value="KAI7726517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BNA2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000523">
        <property type="entry name" value="Zinc finger protein WIP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055187">
        <property type="entry name" value="C2CH-3rd_BIRD-IDD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043584">
        <property type="entry name" value="WIP1/2/3/4/5/6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059161">
        <property type="entry name" value="Znf-C2H2_STOP1/2_3rd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45878:SF51">
        <property type="entry name" value="ZINC FINGER C2H2-TYPE_INTEGRASE DNA-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45878">
        <property type="entry name" value="ZINC FINGER PROTEIN WIP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22995">
        <property type="entry name" value="C2CH-3rd_BIRD-IDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23115">
        <property type="entry name" value="zf-C2H2_STOP2_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="213"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="297"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="64"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="160"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="64"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="83"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="96"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="108"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="122"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7726517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="372" mass="42329" checksum="42FD945A99869ADE" modified="2024-05-29" version="1">MTDPYSNFYSKWFKLDQLTTNSPTNLPHHVQSNRPYQHPQSNYYYNNLHPSSYVNIRTIQGFNFSPSTSINDQHPSPPPSPPLREALPLLSLSPSRKQEDIHHHQEKQPSCTSTNYQHMDVDGDDVDEEDEDDETEVTVALHIGLPKASAADLASLMSKSSSTSSEIMVPNDSKSKEGDGDEGSLVYSPISRLNKGQYWIPTPSQILIGPTQFTCPVCFKNFNRYNNMQMHMWGHGSQYRKGPESLRGTQPSGMLKLPCYCCAPGCRNNIDHPRTRPLKDFRTLQTHYKRKHGTKPFMCRKCGKAFAVRGDWRTHEKNCGKLWYCICGSDFKHKRSLKDHIKAFGNGHAAYGINVFEEEDEPASEIEQDDMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BNE7</accession>
    <name>A0AAD5BNE7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_007040</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyltransferase 31 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011926">
        <property type="protein sequence ID" value="KAI7725498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BNE7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016758">
        <property type="term" value="F:hexosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002659">
        <property type="entry name" value="Glyco_trans_31"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01762">
        <property type="entry name" value="Galactosyl_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12" id="KW-0464">Manganese</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7725498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="71" mass="8033" checksum="3D73F8F93B9584FD" modified="2024-05-29" version="1">MKSGEVFSEQGQKWYEPDWWKFGDGKTYFRHASGEMFVISKALARFVSINRSILRTYAFDDVSTGGICAGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BRS4</accession>
    <name>A0AAD5BRS4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_002381</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011470">
        <property type="protein sequence ID" value="KAI7727009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BRS4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7727009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="173" mass="19789" checksum="0E2FC4A4080EB77A" modified="2024-05-29" version="1">MIQQALEHMMVHREISSKSSPFVIVKPPISANRRHRIEPQSRFCLKKKHKTRCNPFSIPNPNFHSSIVATTKLLLIIVFKLMLDDFQMLVNQIIDLVRSNCMKFPSLTQRLEYYIRSSTRVKNYNAIRVIAANGARSNENGGAKDIYGWMSVSFADNGHVLGGGCAQWWNLKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BS28</accession>
    <name>A0AAD5BS28_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_000157</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 17 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011213">
        <property type="protein sequence ID" value="KAI7728452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BS28"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000010">
        <property type="entry name" value="glucan endo-1,3-beta-glucosidase, basic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000490">
        <property type="entry name" value="Glyco_hydro_17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044965">
        <property type="entry name" value="Glyco_hydro_17_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32227">
        <property type="entry name" value="GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00332">
        <property type="entry name" value="Glyco_hydro_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00587">
        <property type="entry name" value="GLYCOSYL_HYDROL_F17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7728452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="315" mass="34905" checksum="55BECC09CDFCF8F5" modified="2024-05-29" version="1">MLGNDLPSATDVISLYNSNGIGRMRLYDPNQAALQALSGSNIEVMVGVPNSDLQYVAASRDNSFDWVWKNIMSYPDVKFRYISVGNEVKPSDGTLAPLVYSALTNIHEVVVFYGLKNQIKVSTSIDTTLIGANYPPSQGAFRDEYRAYIDPIIAFLVANNAPLLVNVYPYFSYVDNPVDISLAYATFTSPGIVVQDGAHGYQNLFDAIVDVVYSALEKAGGPWVEIVVSETGWPSDGGFAATFDNARAYLTNMVAHARWGTPKRPGRAIETYLFAMFNENNKEPQIEKNFGLFYPNQQAKYNLYFNSIQKGFESI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BSG0</accession>
    <name>A0AAD5BSG0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_022645</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011133">
        <property type="protein sequence ID" value="KAI7728823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BSG0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="228"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="268"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7728823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="308" mass="35855" checksum="895DB967FBE56753" modified="2024-05-29" version="1" fragment="single">MPDKETRRLGFRVRKNLTYEEVLELYVGAPSLFSIKLHHNGRFTRFPERTYVEGEENFIDLFDHDKFSVHEMDRSDLDFGLRALGSDADVIGFIKVIPECRVEEITDEKLMTFDLDKMVRNRLLLCWHGDDKPEANQGVVDNSDEVVVNQEHTQSMHTDEVMDNHVHNVHTEEVVDNQLHNMHIEEVVVNHVDNMHTEQVVENMNFHVAFDFSNIDLKDPNFDPFFSESPTVNVPKHNVPKRNQPQDNMHEHDNENVDDGMNISDHGSDDDNASEDSDFLVDEGNLINEVETNMRDFRFFTDDVEDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BTA5</accession>
    <name>A0AAD5BTA5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Endoplasmic reticulum transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_002197</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May play a role in anterograde transport of membrane proteins from the endoplasmic reticulum to the Golgi.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the BCAP29/BCAP31 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011029">
        <property type="protein sequence ID" value="KAI7729258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BTA5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070973">
        <property type="term" value="P:protein localization to endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008417">
        <property type="entry name" value="BAP29/BAP31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12701">
        <property type="entry name" value="BCR-ASSOCIATED PROTEIN, BAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12701:SF56">
        <property type="entry name" value="ENDOPLASMIC RETICULUM TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="1" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0653">Protein transport</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="77"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU367026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7729258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="249" mass="28322" checksum="018783292BFDAC1F" modified="2024-05-29" version="1" fragment="single">KARHLNHDVVGDQSVNRVLEIRRRFVTFVNAVDSPDHIVLAYHMIEACLMGFSLFLLVTVNSLHQYIKEVIMVKETIRAAKKQNQAYEDCMKKSAEEAEIIRKDISRLKTEIANLESECSLKEQAAQLLRADSSILKNKLEGCLEKYDRLLTYNKDLKEQLQGVNERSSHSNVGIGGACDGKTGKPVNWISRPSLLELRTCFLNQPQPSISCPIQCESYSSDLLRLPLNLLYNEFVYNSSNLLCFLLRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BU77</accession>
    <name>A0AAD5BU77_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Allene oxide synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_020927</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010930">
        <property type="protein sequence ID" value="KAI7729722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BU77"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009941">
        <property type="term" value="C:chloroplast envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009695">
        <property type="term" value="P:jasmonic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016125">
        <property type="term" value="P:sterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11071">
        <property type="entry name" value="CYP74"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000024">
        <property type="entry name" value="Allene oxide synthase, chloroplastic"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002403">
        <property type="entry name" value="Cyt_P450_E_grp-IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24286">
        <property type="entry name" value="CYTOCHROME P450 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24286:SF330">
        <property type="entry name" value="CYTOCHROME P450 SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00465">
        <property type="entry name" value="EP450IV"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0349">Heme</keyword>
    <keyword evidence="3 5" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="5">
        <location>
            <position position="1106"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR602403-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7729722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1154" mass="130400" checksum="8D4C1306DAFC9B4B" modified="2024-05-29" version="1" fragment="single">AIPVYIYNYTLTSIIQNKHTIFTHSSNIWIHRLKPFSVKSRVPTAFPLSNRSKTGWSISTGPEAETIEKKDLFTGTYMPSTKLTGGYRVLSYLDPSEPRHAQLKNLLFFMLKNASNRVIPQFQTTYTELFQSLETELDKNGKAAFNDVGEQAAFRFLGRAYFNSNPEETKLGTSAPTLISSWVLFNLGPSLDLGLPWFLQELLLHTFRLPSFLIKSSYNKLYDYFESVATMVIEQAEKLGVPKDEAVHNILFAILFPNTLKWIGLAGENLHTQLAEEIRGAIKSYGGGKVTLEAIEQMPLTKSVVYESLRIEPPVPPQYGKAKSNFTIQSHDATFQVKEGEMLFGYQPFATKDPKVFDRPEEFVPDRFVGDGEALLKYVWWSNGPETESPTVENKQCAGKNFVVLITRLFVIELFRRYDSFEIELGESPLGAANKEIKRDGHLGRGVVSQDQRGYYNNRTSKELANNHKTSNSEIKVYNVVASLHCDKIKDCTSLEYLQEIQIWSTDFVPTSHIGQADESLLSIIRRRFMTENTSSLLPTASFHLSFSVNKELKRDGHLGRGVGWSGSKIKGYNGKIKDRTSLAYLQEIEIWSADFVPTSHIGQQMNRYLLPDWLMPHECLTGLHFKFHPSTPRDIIIDSDSTTYFFLHFSLFIESSSFIQIRTPFSHIHIHNIFSNSNMDSSSEAPLREIPGSYGIPFIQPFKDRLEYFYGTGGPDGFFQSRAQKYQSTVFRSNMPPGPFISSNPKVIVLLDAKSFPILFDVSRVEKKDLFTGTYMPSTKLTGGYRVLSYLDPSEPRHAQLKNLLFFMLKNSSNRVIPQFQTTYTELFEGLEAELAKNGKAAFNDVGEQAAFRFLGRVYFESNPEETKLGTSAPSLITTWVLFNLSPLGTAGLPWFLEDPLLHTFRLPSFLVKSNYNKLYDYFESVATPLVEQAEILGVPKEEALHNILFAVCFNTFGGMKILFPNTLKWIALAGVNLHTQLAEEIRGAIKSYGDGKVTLDAMERMPLMKSVVYESLRIEPPVPPQYGKAKSDFTIESHDATFEIKKGEMLFGYQPFATKDPKVFDRPEEFVPDRFVGDGEALLKYVFWSNGPETESPTVGNKQCAGKNFVVLITRLFVVELFRGYDTFEVELGAISALGAGITFTSLKKANT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BVC9</accession>
    <name>A0AAD5BVC9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_001428</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010835">
        <property type="protein sequence ID" value="KAI7730140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BVC9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04873">
        <property type="entry name" value="ACT_UUR-ACR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043561">
        <property type="entry name" value="LHW-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025610">
        <property type="entry name" value="MYC/MYB_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46196:SF24">
        <property type="entry name" value="MYC-TYPE, BASIC HELIX-LOOP-HELIX (BHLH) DOMAIN, TRANSCRIPTION FACTOR MYC_MYB N-TERMINAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46196">
        <property type="entry name" value="TRANSCRIPTION FACTOR BHLH155-LIKE ISOFORM X1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14215">
        <property type="entry name" value="bHLH-MYC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23176">
        <property type="entry name" value="bHLH_LHW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="6">
        <location>
            <begin position="279"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="211"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="261"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="280"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7730140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="451" mass="50942" checksum="D0F120E7112856C3" modified="2024-05-29" version="1">MVMMMSGGELHQQLKNLCFNTDWKYAVFWKLKHQPQMMLSWEDAYCNDKEKNDCSEINKWFSNADDNFKDEWYPQDHLGLAIANMSYRIYSIDEGVVGRVAATGKHQWISGIQLVNSLCSMDEHSDGWKTQVTAGIRETETNNSFKFPAGCELYEALGPTFYKQKNCFDWETVTTETLTVDQMAGDTNSTQRSDSENLLEAVVASVCCSDSDTKWSKSSGQPVKPLSRLQPTPSDLQTGSSICYSFERSLQGFSSASVSRCSEQLDAPREPPKAGKKRARPGESGKPRPRDRQLIQDRIKELRQLVPNGSKCSIDSLLERTIKHMLFMQCITKHADKIDKCAEFKLHGNQEQGSSWAMEVGTDLKVCPIIVENIGTEGQMLIEMVCDEGVNFLEITEAIRSLGLTILKGAIDAYGDKTWMCFIVEGENNRSIHRMDILWSLVQILELKTKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BVV8</accession>
    <name>A0AAD5BVV8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_030152</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010724">
        <property type="protein sequence ID" value="KAI7730592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BVV8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009506">
        <property type="term" value="C:plasmodesma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004714">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045272">
        <property type="entry name" value="ANXUR1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27003">
        <property type="entry name" value="OS07G0166700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27003:SF363">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="189"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="360"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="205"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="255"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="288"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="304"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="333"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="360"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="390"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="438"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="450"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7730592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="464" mass="50726" checksum="05D880B4F9DCFB74" modified="2024-05-29" version="1" fragment="single">QRHQICLGAARGLEYLHSGDESVNSNPCIIHGNLKLSKILLNNDAVSSNFEAKVSGFGLPKLLPGHVEIGPRTPEKLTKESDVYSFGVILLEVLCGVPELVDTDDYQERHVAELVPKRLEQNMLRKIVHFDIRDEITTESLETYAKIACQCVRENPEERPTMSEVVIELEKALRLQGGKVSDVQIFGSSGIGQPDVAVGEDDHEGETMRSDITEEEPKRNEETTDVNDKDINLGGKVSDKLLASSNGNGLSEVPVGEDDQEGDDNGETMSSTITEEESKEDAEKENVNVPNGTEETTVDLNSNEESKIVGENKVNDESKITTSSSSYANEPLPDERSATKEKGKGLMMMNTHNPDIMEVQNSAVSDSNGKAGSLNTEKPEVIINDRGGDYSNSAIQIQESESKRSNSTCSESSNVDDTAASDYLLPKSPDNVGENWTDLGQAQNQNMKMLDPRSRPSKIDQGNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BWB9</accession>
    <name>A0AAD5BWB9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_002280</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010693">
        <property type="protein sequence ID" value="KAI7730642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BWB9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071006">
        <property type="term" value="C:U2-type catalytic step 1 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071007">
        <property type="term" value="C:U2-type catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036002">
        <property type="term" value="F:pre-mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017070">
        <property type="term" value="F:U6 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12224">
        <property type="entry name" value="RRM_RBM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000036">
        <property type="entry name" value="Zinc finger CCCH domain-containing protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000347">
        <property type="entry name" value="Zinc finger CCCH domain-containing protein 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039171">
        <property type="entry name" value="Cwc2/Slt11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048995">
        <property type="entry name" value="STL11/RBM22-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032297">
        <property type="entry name" value="Torus"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14089">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR RBM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14089:SF6">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR RBM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21369">
        <property type="entry name" value="STL11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16131">
        <property type="entry name" value="Torus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 7" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="10">
        <location>
            <begin position="157"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="228"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="7">
        <location>
            <begin position="157"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="330"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="412"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="423"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7730642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="455" mass="51206" checksum="B8D49E9749B7EBCC" modified="2024-05-29" version="1">MAHRVLRDVEADGWERSDFPIICESCLGDNPYIRMTKENYGAECKICTRPFTVFMWRPGRDARYKKTEICQTCSKLKNVCQVCVLDLEYGLPVQVRDTALSISSHDSIPKSDVNREYFAEEHDRRARAGLDYESSYGKVRPNDTLLKLQRTTPYYQRNRAHICSFYIRGECTRGAECPYRHEMPVTGELSQQNIKDRYYGINDPVALKLLNKAGDMPALEPPDDESIRTLYVGGLDARISEQDLRDEFYSHGEIESIKMVVQKACAFVTYTTREGAEKAAAELSNKLVVKGLRLKLMWGRPQAAKLDGETGLSAAHSGLLPRAVISHQQYQDVPPPGTSTSQDQQLPPHMQYFNIPPPPPQYERTFYPSMDPQRMGAAIPNQEGGGSGGSTENKGDPGLQYAAYPPPPLPPHGGDQYYQQYYAPPPYGYMQPPPEQSYQQYPSTVPPTAPTDEQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BXB8</accession>
    <name>A0AAD5BXB8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribulose bisphosphate carboxylase large chain</fullName>
            <ecNumber evidence="3">4.1.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">M8C21_032208</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">AA19_3_7</strain>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate in the photorespiration process. Both reactions occur simultaneously and in competition at the same active site.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>2 (2R)-3-phosphoglycerate + 2 H(+) = D-ribulose 1,5-bisphosphate + CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:23124"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57870"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="EC" id="4.1.1.39"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>D-ribulose 1,5-bisphosphate + O2 = 2-phosphoglycolate + (2R)-3-phosphoglycerate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:36631"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57870"/>
            <dbReference type="ChEBI" id="CHEBI:58033"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RuBisCO large chain family. Type I subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.39" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010010829">
        <property type="protein sequence ID" value="KAI7730193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BXB8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016984">
        <property type="term" value="F:ribulose-bisphosphate carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009853">
        <property type="term" value="P:photorespiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019253">
        <property type="term" value="P:reductive pentose-phosphate cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.150">
        <property type="entry name" value="RuBisCO large subunit, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033966">
        <property type="entry name" value="RuBisCO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017443">
        <property type="entry name" value="RuBisCO_lsu_fd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036422">
        <property type="entry name" value="RuBisCO_lsu_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42704">
        <property type="entry name" value="RIBULOSE BISPHOSPHATE CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42704:SF15">
        <property type="entry name" value="RIBULOSE BISPHOSPHATE CARBOXYLASE LARGE CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02788">
        <property type="entry name" value="RuBisCO_large_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54966">
        <property type="entry name" value="RuBisCO, large subunit, small (N-terminal) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0113">Calvin cycle</keyword>
    <keyword evidence="13" id="KW-0120">Carbon dioxide fixation</keyword>
    <keyword evidence="5" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="12" id="KW-0456">Lyase</keyword>
    <keyword evidence="10" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-0601">Photorespiration</keyword>
    <keyword evidence="6" id="KW-0602">Photosynthesis</keyword>
    <keyword evidence="8" id="KW-0934">Plastid</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribulose bisphosphate carboxylase large subunit ferrodoxin-like N-terminal" evidence="17">
        <location>
            <begin position="36"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00025664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF02788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAI7730193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="74" mass="8464" checksum="EE970DCE21E8FE74" modified="2024-05-29" version="1">MMSLKITNREVFMSPQTETKASVGFKAGVKDYKLTYYTPEYETKDTDILAAFRVTPQPGVPASSIILVELYYRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BXJ6</accession>
    <name>A0AAD5BXJ6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Histone deacetylation protein Rxt3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_027302</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010488">
        <property type="protein sequence ID" value="KAI7731255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BXJ6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.130.20">
        <property type="entry name" value="LCCL-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036609">
        <property type="entry name" value="LCCL_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013951">
        <property type="entry name" value="Rxt3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08642">
        <property type="entry name" value="Rxt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69848">
        <property type="entry name" value="LCCL domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="143"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="143"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="199"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="229"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="265"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="344"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="428"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7731255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="757" mass="85655" checksum="1DE8355DF8419869" modified="2024-05-29" version="1">MSGTPNKRVHEDSGGHSSLSRYSHPPEDSGTYSGMGGANPKLPNTSAPSDYHTSFDMGQDSRMPKVPRTELDSRRTPLLPMYRMQTSSLNDSQSDHAVNPEERAELRDARDSSVRDMKVENRDVNAESKELYQGAKIEKDVRYAGRGDDHKDTKYERNAYSDYRNEVKTDKDGYNPVNSNVNLKESKEHHSRPVWKYGDTTSGNMDSWHASRSNVHGQVEVGREGLNTEGKDYNTEAHETVSENKVDLKGDDALKERDRKRKDVKHREWGDNNKERTEGRNNEMKDTTKEERGGEKWEKDRKDVLLKDKDKVKEHIKRDSWVANEKEGQHEKEDVDVSSTRALDLGKQKSTDNWKDLDKESKERGTEKDADMEGERSERRNKGFDKDSDDGGADVEGSADREREGFSYGVQQRKRMLRPRGSPQVGNRDSRFRSRQDNDGSSGKSDVSTVVYRVGECMQELLNLWKEYKSSNIADKPSESSKSFPTLEIRIPAEHVTATNRQVKGGQLWGTDIYTHDSDLVAVLMHTGYCRPTASPPPPAILELRATVKVLPPQDCYVSTLRNNVRSRAWGAAIGCGCSFRVERCHIVKKAGGTIDLEPCLTHTSTVEPTLAPVVVERTMTTRAAASNALRQQRFVREVTLQYNLCNEPWIKYSISAIADKGLKKPLFTSARLKKGEVLYLESPRQRFELCFNGEKLVKAGNEMDTNGVDSDNTTAHNNNNNNNMMDVFRWSLCKKPLPQTVMRSIGIPLPPEHLKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BYC2</accession>
    <name>A0AAD5BYC2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Casein kinase II subunit beta</fullName>
            <shortName evidence="2">CK II beta</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_027004</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Plays a complex role in regulating the basal catalytic activity of the alpha subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the casein kinase 2 subunit beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010413">
        <property type="protein sequence ID" value="KAI7731645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BYC2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005956">
        <property type="term" value="C:protein kinase CK2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019887">
        <property type="term" value="F:protein kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1820.10">
        <property type="entry name" value="protein kinase ck2 holoenzyme, chain C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016149">
        <property type="entry name" value="Casein_kin_II_reg-sub_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035991">
        <property type="entry name" value="Casein_kinase_II_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000704">
        <property type="entry name" value="Casein_kinase_II_reg-sub"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11740">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11740:SF0">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01214">
        <property type="entry name" value="CK_II_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00472">
        <property type="entry name" value="CASNKINASEII"/>
    </dbReference>
    <dbReference type="SMART" id="SM01085">
        <property type="entry name" value="CK_II_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57798">
        <property type="entry name" value="Casein kinase II beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="20"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="35"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="50"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="75"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU361268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7731645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="150" mass="16950" checksum="5995488388ED93DD" modified="2024-05-29" version="1" fragment="single">MYRDRRSSEMVGGGTGTASFDRKRINDALDKQLEKTSSVSSVKVFNNNNNKEKEKDKEKLSVPSSSKIATTKNDDSDEESETDSEEESDVSGSDEDDTSWISWFCNLRGNEFFCEVDDEYIQDDFNLCGLSSQVPYFDYALDLILDVESS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BYH4</accession>
    <name>A0AAD5BYH4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phorbol-ester/DAG-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_011335</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010377">
        <property type="protein sequence ID" value="KAI7731812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BYH4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004146">
        <property type="entry name" value="DC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053192">
        <property type="entry name" value="Vacuole_Formation_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32410">
        <property type="entry name" value="CYSTEINE/HISTIDINE-RICH C1 DOMAIN FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32410:SF216">
        <property type="entry name" value="PHORBOL-ESTER_DAG-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03107">
        <property type="entry name" value="C1_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="6">
        <location>
            <begin position="470"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="6">
        <location>
            <begin position="522"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="15"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="28"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7731812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="662" mass="76945" checksum="B9B7AE597D17EAE2" modified="2024-05-29" version="1">MEQLQHFTHEHMLSLVQLQPDHTNENSDKEDEDENEDKDEVVVEDPLVGKCEMCKEEIYSFHRSYYSCKDCDYSLHKFCAELPTTQQNHPLHPGHNLTLSQNFQVQDPDVNFIETFDSEWICHVCEIDRTMFYNYHCYICKFTMDIVCATMSQQKMNHPSHPHQLQRDLKWLTSHCDACGKKHSGVFYHCTTCLWFRIHLDCALLPAKLLIQKSTNGSFSHLHLLTLYYSPFPYIEQKAKFFPRCRVCDEFFTGNRWYYGCHKCQYYTHVRCATSGPEEAFMSIIRPGGHGMTYKNFKDGDHPNLIHCPFPDESANLLTHQFANKGELVINETIDGQMLSHQHPLILVKTLLNESVSLNDPMKRVELLCDGCVRPITALPFYKCSQHNCGFVLHEWCTRLPFEILDHPDHPEHKLVLLPKMPGNLLGVFLCAICRFYCNGFAYGCIECKYYVDINCGFIPDVITHEAHPNHLLLRFKASAHQPKQGCKACYTYIVTPLGFHCPTCDFNLHTECALSIPRTIRHKFDKHPLSLRYYPAENYSSDYFCEICEDVLNPTKWFYHCNMCASSIHTQCAPVKLECERSTSSWINCSIFAYYNVKFGGRYKMRDHPHLVMFVQGIKDDGQCIVCDEPLQYEMIFKCFECKFALHFGCEEPDVLVIPPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C1H7</accession>
    <name>A0AAD5C1H7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="9">Methylthioribulose-1-phosphate dehydratase</fullName>
                <shortName evidence="9">MTRu-1-P dehydratase</shortName>
                <ecNumber evidence="9">4.2.1.109</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="9">Enolase-phosphatase E1</fullName>
                <ecNumber evidence="9">3.1.3.77</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="9">2,3-diketo-5-methylthio-1-phosphopentane phosphatase</fullName>
            </alternativeName>
        </domain>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_018624</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>5-(methylsulfanyl)-D-ribulose 1-phosphate = 5-methylsulfanyl-2,3-dioxopentyl phosphate + H2O</text>
            <dbReference type="Rhea" id="RHEA:15549"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58548"/>
            <dbReference type="ChEBI" id="CHEBI:58828"/>
            <dbReference type="EC" id="4.2.1.109"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>5-methylsulfanyl-2,3-dioxopentyl phosphate + H2O = 1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + phosphate</text>
            <dbReference type="Rhea" id="RHEA:21700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:49252"/>
            <dbReference type="ChEBI" id="CHEBI:58828"/>
            <dbReference type="EC" id="3.1.3.77"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="9">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="9">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="9">Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 2/6.</text>
    </comment>
    <comment type="pathway">
        <text evidence="9">Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 3/6.</text>
    </comment>
    <comment type="pathway">
        <text evidence="9">Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 4/6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">In the C-terminal section; belongs to the HAD-like hydrolase superfamily. MasA/MtnC family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">In the N-terminal section; belongs to the aldolase class II family. MtnB subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.109" evidence="9"/>
    <dbReference type="EC" id="3.1.3.77" evidence="9"/>
    <dbReference type="EMBL" id="JAMZMK010010016">
        <property type="protein sequence ID" value="KAI7733372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C1H7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043874">
        <property type="term" value="F:acireductone synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046570">
        <property type="term" value="F:methylthioribulose 1-phosphate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019509">
        <property type="term" value="P:L-methionine salvage from methylthioadenosine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01629">
        <property type="entry name" value="HAD_EP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.720.60:FF:000001">
        <property type="entry name" value="Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.225.10:FF:000010">
        <property type="entry name" value="Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000088">
        <property type="entry name" value="Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.225.10">
        <property type="entry name" value="Class II aldolase/adducin N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03116">
        <property type="entry name" value="Salvage_MtnB_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03118">
        <property type="entry name" value="Salvage_MtnBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001303">
        <property type="entry name" value="Aldolase_II/adducin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036409">
        <property type="entry name" value="Aldolase_II/adducin_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023943">
        <property type="entry name" value="Enolase-ppase_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017714">
        <property type="entry name" value="MethylthioRu-1-P_deHdtase_MtnB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027505">
        <property type="entry name" value="MtnB_viridiplantae"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027514">
        <property type="entry name" value="Salvage_MtnB_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01691">
        <property type="entry name" value="enolase-ppase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03328">
        <property type="entry name" value="salvage_mtnB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20371">
        <property type="entry name" value="ENOLASE-PHOSPHATASE E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20371:SF1">
        <property type="entry name" value="ENOLASE-PHOSPHATASE E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00596">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01133">
        <property type="entry name" value="C1.5.4:_Enolase-phosphatase_Li"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00044">
        <property type="entry name" value="enolase-phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01007">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53639">
        <property type="entry name" value="AraD/HMP-PK domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="3 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 9" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="8 9" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Class II aldolase/adducin N-terminal" evidence="10">
        <location>
            <begin position="26"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Methylthioribulose-1-phosphate dehydratase" evidence="9">
        <location>
            <begin position="1"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Enolase-phosphatase E1" evidence="9">
        <location>
            <begin position="266"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor; for methylthioribulose-1-phosphate dehydratase activity" evidence="9">
        <location>
            <position position="152"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>substrate</name>
            <label>1</label>
            <note>for methylthioribulose-1-phosphate dehydratase activity</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="269"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="271"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="404"/>
            <end position="405"/>
        </location>
        <ligand>
            <name>substrate</name>
            <label>2</label>
            <note>for enolase-phosphatase activity</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="438"/>
        </location>
        <ligand>
            <name>substrate</name>
            <label>2</label>
            <note>for enolase-phosphatase activity</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="464"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7733372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="505" mass="55898" checksum="153F408641A483C7" modified="2024-05-29" version="1">MTTNGIKMAVTSQAYLEGSQVKQTKSLITELCRLFYTLGWVSGTGGSITAKVHDDSVPKSDQLIIMSPSGVQKERMVEEDMYVLSSVGDILFTPLPKAYPHKPPKCSDCAPLFMKAYHMRNAGAVIHSHGMESCLVTMLDPLAKEFRITHMEMIKGIQGHGYYDELVVPIIENTAHERELTESLAAAIEAYPKTTAVLVRNHGIYVWGDSWISAKTQAECYHYLFDAAIKLHQLGLDWSTPSHGPIQRSKPLLGGQTTNTSQKRCIVLDIEGTTTPITFVTDVLFPYARNNVQRHLEETYDTDFTQDDIKLLRSQVEDDLQHGVAGAVPIPSDEAGKQAVIAALVANVEGMIKSDRKITSLKQLQGHIWRTGFEKNELKGVVFDDVPGALEKWHASGTKVYIYSSGSRLAQRLLFGYTNYGDLRTYLCGYFDTTIGNKKETKSYVEISESLGVHKPSDILFVTDVYQEAVAAKAAGLEVVISVRPGNGTLPENHGFRTIKSFSEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C558</accession>
    <name>A0AAD5C558_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">LIM zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_004943</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009569">
        <property type="protein sequence ID" value="KAI7735074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C558"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09396">
        <property type="entry name" value="LIM_DA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000107">
        <property type="entry name" value="Protein DA1-related 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045218">
        <property type="entry name" value="DA1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022087">
        <property type="entry name" value="DA1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24209">
        <property type="entry name" value="PROTEIN DA1-RELATED 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24209:SF38">
        <property type="entry name" value="TRANSCRIPTION FACTOR INTERACTOR AND REGULATOR LIM FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12315">
        <property type="entry name" value="DA1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0440">LIM domain</keyword>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="7">
        <location>
            <begin position="138"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="41"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="45"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="71"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7735074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="498" mass="56369" checksum="188E32BEB5AAFD9B" modified="2024-05-29" version="1">MSYSGNVRHLSQPCINGDIFSSHTERKSRFMKWMNKLFKGGSNRGPVNSGNPSPQFIGDGSMVSRAPVRSLDNRSRSDKEKEELDRAIALSLAEGMKKPNGYGWQPSNNEDLSKSHEDNFHPPYPSYMPSEYNPTEYRICGGCNRDIGYSSYLGCMGTFFHPECFRCHACGYPITEPEFSVSEKESYHKSCFKDLTHPKCEVCFQYIPTNKDGLIEYRCHPFWSQRYCPAHEHDNTARCCSCERLESVNVRYISLGDGRSLCLECMESAIMDTGDCQPLYHSIRDFYEGMNMRLDQQIPMLLVERHALNDAIDKEKHGFHHMPETRGLCLSEELTVASVQRRPRIGGNRFIGMRTRHQKLTRKCEVTAILVLYGLPRLLTGSILAHELMHGWLRLNGYRNLNPEIEEGICQVLAHMWLESEIMPGLKSMPSTSSSSYGSKKGGISRADNKLGEFFLHQIANDASPAYGGGYRAANAAVTTYGLRRTLDHIRLTGSIPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C7A1</accession>
    <name>A0AAD5C7A1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Phosphoinositide phospholipase C</fullName>
            <ecNumber evidence="4 11">3.1.4.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">M8C21_008334</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">AA19_3_7</strain>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + H2O = 1D-myo-inositol 1,4,5-trisphosphate + a 1,2-diacyl-sn-glycerol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:33179"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
            <dbReference type="ChEBI" id="CHEBI:203600"/>
            <dbReference type="EC" id="3.1.4.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.11" evidence="4 11"/>
    <dbReference type="EMBL" id="JAMZMK010009492">
        <property type="protein sequence ID" value="KAI7735394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C7A1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004435">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate phospholipase C activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051209">
        <property type="term" value="P:release of sequestered calcium ion into cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00275">
        <property type="entry name" value="C2_PLC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08599">
        <property type="entry name" value="PI-PLCc_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000060">
        <property type="entry name" value="Phosphoinositide phospholipase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001192">
        <property type="entry name" value="PI-PLC_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000909">
        <property type="entry name" value="PLipase_C_PInositol-sp_X_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001711">
        <property type="entry name" value="PLipase_C_Pinositol-sp_Y"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10336:SF187">
        <property type="entry name" value="PHOSPHOINOSITIDE PHOSPHOLIPASE C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10336">
        <property type="entry name" value="PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE C FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00388">
        <property type="entry name" value="PI-PLC-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00387">
        <property type="entry name" value="PI-PLC-Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00390">
        <property type="entry name" value="PHPHLIPASEC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00148">
        <property type="entry name" value="PLCXc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00149">
        <property type="entry name" value="PLCYc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50007">
        <property type="entry name" value="PIPLC_X_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50008">
        <property type="entry name" value="PIPLC_Y_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 11" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="8 11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0807">Transducer</keyword>
    <feature type="domain" description="PI-PLC Y-box" evidence="14">
        <location>
            <begin position="237"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="13">
        <location>
            <begin position="325"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="174"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="185"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAI7735394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="472" mass="53399" checksum="1D53304930659DDB" modified="2024-05-29" version="1">MTAPLSHYFIYTGHNSYLTGNQLSSKSSELPIIKALKRGVRVIELDLWPNSSKDDVNVLHGRTLTSSVKLRDCLKAIKEYAFVASPYPVIITFEDHLTPDLQAKVAKMVTETFGSMLFSPESENLEELPAPENLKHKILISTKPPKECREAGDIKRSSSQNIQVFDVDVDDAWSEDESSKSGSDMSDHDVETEDLLKGKKSPSSPVYKKLIAIHAGKPKNGLKNALKAEKDKVRRLSLVEQALKKAAENHGQDVVRFTQKHILRIYPKGTRITSSNYKPLTCWLHGAQMVAFNMQGYGRSLWLMHGMFRANGNCGYVKKPEFLIRCGSKNEVFNPKAKLPPKTSLKVKVYMGDGWHLDFKKTHFDRYSPPDFYTRVGIAGASVDRIMKKTKIKVDNWTPVWNEEFTFPLAVPELALLRFEVHDRNVSEKDTFAGQICLPVSELRPGIRAVPLCNRKGDQYPSARLLMRFEFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C854</accession>
    <name>A0AAD5C854_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_013309</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009114">
        <property type="protein sequence ID" value="KAI7737101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C854"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11073">
        <property type="entry name" value="CYP76-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000007">
        <property type="entry name" value="Cytochrome P450 76C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47950">
        <property type="entry name" value="CYTOCHROME P450, FAMILY 76, SUBFAMILY C, POLYPEPTIDE 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47950:SF4">
        <property type="entry name" value="GERANIOL 8-HYDROXYLASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7 8" id="KW-0349">Heme</keyword>
    <keyword evidence="5 7 8" id="KW-0408">Iron</keyword>
    <keyword evidence="3 7 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 8" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="7">
        <location>
            <position position="434"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7737101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="492" mass="55440" checksum="66FEECC2B12D09AA" modified="2024-05-29" version="1">MDILVFVVVVVFFSYAILSIFGFGKPMNLPPGPTPLPIIGNLHLLGRQPHRSLAKLAHTHGPVMLLKLGQNTTLVISSIAAAKEVLQKQDLAFSSTRHIPDSLTIHNFSKHSLGFLPIGTRWRFLRRILNTNIFSGKSLEASQHLRDQKVQELIDYCNKACLSNDYVDIGHVAFTTSLSLLSNTIFSKDLTCPYEDSTNDFREVVGNIMEEAGKPNLVDFFPVLKVFDPQGIRRRMTRHVGKVLSILEELIEERFAMGGSKKDDVLEMCLRINQDNPEELSKSLIKFLILDMFIAGTHTLSSTVEWAMAELLRNPHIMAKSKEELEQVVGKGKIIKEHDFLRLPYLSSIVKETFRLHPPAPLLLPRKAENQVKLNGYIIPKGTQVLVNAWAIGQDPIIWEDSKEFKPERFLDCQIDVRGQDFELIPFGAGRRICPGMPLALSTIHMMLGSLPNNFDWKLDSNVHPKELDMTERFGITLQIANPISVVPIPLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C9D8</accession>
    <name>A0AAD5C9D8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_009039</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TALE/BELL homeobox family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009134">
        <property type="protein sequence ID" value="KAI7737048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C9D8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008422">
        <property type="entry name" value="KN_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006563">
        <property type="entry name" value="POX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050224">
        <property type="entry name" value="TALE_homeobox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11850">
        <property type="entry name" value="HOMEOBOX PROTEIN TRANSCRIPTION FACTORS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05920">
        <property type="entry name" value="Homeobox_KN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07526">
        <property type="entry name" value="POX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0371">Homeobox</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="POX" evidence="9">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="KN homeodomain" evidence="8">
        <location>
            <begin position="108"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF05920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7737048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="140" mass="15399" checksum="987532B2A1DFE673" modified="2024-05-29" version="1">MQMVISSFETVAGLSAATPYVCLALKAVSRHFHHVKNVISEQLSQMRKTVGEGLCASTTVSKTFDANATSTSRQKSGGGSANFFVPQQPVWRPQRGLPERAIAVLKTWLFDHFLHPYPSDADKHMLATQTGLTRNQVNNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CB65</accession>
    <name>A0AAD5CB65_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_031706</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008723">
        <property type="protein sequence ID" value="KAI7738768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CB65"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7738768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="26" mass="3075" checksum="B5785035266C0929" modified="2024-05-29" version="1">MRIVFPESQFGQHRDCREVANRSPQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CC88</accession>
    <name>A0AAD5CC88_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_033857</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008733">
        <property type="protein sequence ID" value="KAI7738743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CC88"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37259">
        <property type="entry name" value="OS07G0474300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37259:SF2">
        <property type="entry name" value="OS07G0474300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="161"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="165"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7738743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="204" mass="23484" checksum="5475D63460AB86D0" modified="2024-05-29" version="1">MTTTMRSDRKPPLGLRSPIRLRPRHPLQSSLPNLQTPSGTVTKSRLPKQPLVMEEPELRPEYHTMSCELNALTKMVQDTLWGSATTNQSGNERPLFQRGKFYEKYSAIRSERLKRKRGESVVEKKTPCKQYLGVRVESAMKKPMEVKKFESARKMATPLMERRQTATTQRYSLRSSCKENKKPPLAMSSLGVATVERKTARKRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CD73</accession>
    <name>A0AAD5CD73_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">M8C21_026624</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">AA19_3_7</strain>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008639">
        <property type="protein sequence ID" value="KAI7739289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CD73"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000225">
        <property type="entry name" value="cold-responsive protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000044">
        <property type="entry name" value="Putative LRR receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052059">
        <property type="entry name" value="CR_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47973">
        <property type="entry name" value="CYSTEINE-RICH RECEPTOR-LIKE PROTEIN KINASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 15 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 15 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 16" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Protein kinase" evidence="18">
        <location>
            <begin position="44"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="331"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="359"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAI7739289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="379" mass="41445" checksum="E2BE5B8647C48A26" modified="2024-05-29" version="1">MGIGCFGAFDSCKAKSRNPPADEIATNNVKQFSYNSLRSATRNFHSDNKIGGGGFGVVYKGVLRDDTHVAIKSLSAESKQGTIEFLTEINMISRIRHPNLVQLVGCCVEDGKRILVYEYLNNNSLAHALLGSRGKQVELDWATRASICRGTASGLAFLHDEAEPRIVHRDIKASNVLLDGNFDPKIGDFGLARLFPDNVTHISTRVAGTVGYLAPEYALLGQLTKKVDVYSFGVLMLEVISCRSSSKSAFGEELLSLVEWAWKLKDEERLLDLVDPDLTDYPDDEVMRFITVALFCTQAVSNQRPSMSQVVEMLSTTVKLNVRLLTEPGIYRRQSSSNGGGGKSLETAKSGGKQAGNPFVTSSPQVTSSYTSVSQMFPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CDE4</accession>
    <name>A0AAD5CDE4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_004181</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008542">
        <property type="protein sequence ID" value="KAI7739968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CDE4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000011">
        <property type="entry name" value="Myb transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000079">
        <property type="entry name" value="MYB transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044676">
        <property type="entry name" value="EOBI/EOBII-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45675:SF123">
        <property type="entry name" value="MYB DOMAIN PROTEIN 62-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45675">
        <property type="entry name" value="MYB TRANSCRIPTION FACTOR-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="8">
        <location>
            <begin position="15"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="7">
        <location>
            <begin position="15"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="8">
        <location>
            <begin position="68"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="7">
        <location>
            <begin position="68"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7739968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="272" mass="31620" checksum="35F6ABA5BCFB9614" modified="2024-05-29" version="1">MRASKKITSSCNEQLDELRRGPWTLDEDNLLIHYITCHGEGRWNSLAKASGLKRTGKSCRLRWLNYLKPDVKHGNLTPQEQILILELHSKWGNRWSKIAQNLPGRTDNEIKNYWRTKVQKQARHLKGDSNSKMFIEALRQFLVPRLLEKVELSTSSSSTSTSSMEVNQNNFNSQPQCKETLMEKTSSNSDIYSSNFKRIVLPYMPESSLQSNGYIVDNNNFDVMSFNQSNTQEFMSSDLSAFEFQMAGIDWTSNEFVDDTFWNMDELWQFKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CDZ9</accession>
    <name>A0AAD5CDZ9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Auxin-responsive protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_015737</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Aux/IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimers and heterodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Aux/IAA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008470">
        <property type="protein sequence ID" value="KAI7740298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CDZ9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009734">
        <property type="term" value="P:auxin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033389">
        <property type="entry name" value="AUX/IAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003311">
        <property type="entry name" value="AUX_IAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053793">
        <property type="entry name" value="PB1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31734">
        <property type="entry name" value="AUXIN-RESPONSIVE PROTEIN IAA17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31734:SF38">
        <property type="entry name" value="AUXIN-RESPONSIVE PROTEIN IAA29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02309">
        <property type="entry name" value="AUX_IAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51745">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0927">Auxin signaling pathway</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0678">Repressor</keyword>
    <keyword evidence="5 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 8" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="PB1" evidence="9">
        <location>
            <begin position="108"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7740298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="190" mass="22525" checksum="4A8F30A49EB38B72" modified="2024-05-29" version="1">MELELSLSPPNQTRHHIKKTATLTRFPLVSWNHDEDDQQPNGFFNGDYQQDHMNEDGEGLTGWPPLHSWRRRLMEENQYERVDDQFNRPVDEVGEHINVMNNNNNYNELFVKVKMEGVGIARKIDLNAFHSYEMLTTALIHMFDKSVEIEEDGASYKLMYQHRDGHWHLGGDLPWEMFIRSVKRIQMVRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CFM0</accession>
    <name>A0AAD5CFM0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kinase and PP2C-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_000495</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008574">
        <property type="protein sequence ID" value="KAI7739729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CFM0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000683">
        <property type="entry name" value="Protein kinase and PP2C-like domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF288">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="28"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="PPM-type phosphatase" evidence="6">
        <location>
            <begin position="380"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="591"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7739729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="591" mass="66233" checksum="E1B91F97FB89B67D" modified="2024-05-29" version="1" fragment="single">LEIIHPNTCIRGCCTSDSIPLHLPPSSHTIFLPIARGAESVVYDATLDGKRVAVKKPVLSTSDDINKFHKELHKLDHEGIATLVAAHARPPNYMFFFKFYEFGNLSQKLHVEEWSPSIDQALMITKQLANALQYLHNIGIVHRDVKPANVLLDKSMKPHLADFGLSEYKQDLQKVTVENWRSSGKPTGGFHKKNMVGTLIYMAPEVLRKEIHTEKSDVYSFGITINELLTGVVPYTDLRTEAQAHTVLEMNYTEQQLTAAVVSEGLRPALAGTESGAPPRLLSLIQRCWDADTQNRPSFDDVVTELDLIIGNNKSFTLEENAVAESSSDDSTKMVQPFREDVNWFSQGENILKTVCESDFDVRSWSNYSKNGSLYVPVLSWGSFATCGRRETMEDTHFLMPRLWNENDIHVFGIFDGHRGGAAAEFSVRALPRLLQTLDCTSSPTDALKEAFINTDVAFRKELDEQRKSKAFIQKDYHPGCTAITALIVQNWLYVANAGDCRAILCRSGCPYPLSKDHVASCLEERERVTGLGGQVKWQLDTWRPWVTAEPEVTETVLSDDDEYMDTVKEPGMCSKRLATEAAERGSNDNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CG31</accession>
    <name>A0AAD5CG31_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">YEATS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_021614</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008223">
        <property type="protein sequence ID" value="KAI7741286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CG31"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16910">
        <property type="entry name" value="YEATS_TFIID14_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1970">
        <property type="entry name" value="YEATS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038704">
        <property type="entry name" value="YEAST_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005033">
        <property type="entry name" value="YEATS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055129">
        <property type="entry name" value="YEATS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47573">
        <property type="entry name" value="PROTEIN AF-9 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47573:SF1">
        <property type="entry name" value="PROTEIN AF-9 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03366">
        <property type="entry name" value="YEATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51037">
        <property type="entry name" value="YEATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="YEATS" evidence="6">
        <location>
            <begin position="5"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="156"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="167"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7741286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="225" mass="25877" checksum="1A7BABBF7089FC6B" modified="2024-05-29" version="1" fragment="single">SSTKKIKDFEICVPIAYGTISFWLGKKATETQSHVWTVYVRGATNEDLGAVIKKVVFQLHPSFSNARRVVESPPFELTERGWGEFEIAISIFFHDDVCDKQLDLFHPLKLYSEVEHGPLSTKKPVIVESYDEIVFPNPSDKFLARVHDHPSVIVPRLPPTMNLPHAPLEDNKRGNKRGDTKDHPHVQWFNKFSEADELLKLAEARQQVQNHIVKLRRRMSMLDNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CH09</accession>
    <name>A0AAD5CH09_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Disease resistance R13L4/SHOC-2-like LRR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_000596</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008134">
        <property type="protein sequence ID" value="KAI7741752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CH09"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000221">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase PXL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000041">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053038">
        <property type="entry name" value="RLP_Defense"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48064">
        <property type="entry name" value="OS01G0750400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48064:SF6">
        <property type="entry name" value="RECEPTOR-LIKE PROTEIN KINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="7">
        <location>
            <begin position="99"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7741752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="329" mass="35978" checksum="376963D6DF6686E9" modified="2024-05-29" version="1">MPKTKTTPSSLQQLSFRSNPTLVGSIPFELFLALSSLKILTLSQSEISGAITPEISNLNSLVHLDLSYNQLSGPIPVELGQLKKLVGLDLSYNLLTGPVPQTIGQLGMLQKLDLSSNSLSGTVPNSIGKLNSLVFMALSNNNIHGKLPIGLRDLKGLEYLIMDNNPMSTELLTEFGRLSKLRELRLANSNYSGEIPASFSQLWNLTTLSLENNRFTGKIPVGIANLSHIYHMNLSKNLLSGEIPFDTSFLKRVGENLDLSGNSELCLNPMQAYECAKLGVDVCNRRHRRVGNSSYEVKPLKTSEGQVVEIGRTSLCFVFLVLGALGLKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CHI6</accession>
    <name>A0AAD5CHI6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">60S ribosomal protein L36</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_022452</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL36 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008113">
        <property type="protein sequence ID" value="KAI7741878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CHI6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1760:FF:000001">
        <property type="entry name" value="60S ribosomal protein L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1760">
        <property type="entry name" value="60S ribosomal protein L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000509">
        <property type="entry name" value="Ribosomal_eL36"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038097">
        <property type="entry name" value="Ribosomal_eL36_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10114">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01158">
        <property type="entry name" value="Ribosomal_L36e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01190">
        <property type="entry name" value="RIBOSOMAL_L36E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="79"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="79"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7741878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="110" mass="12386" checksum="EA8824C3C8CA9869" modified="2024-05-29" version="1">MAPKQPNTGLFVGLNKGHVVTKKELAPRPSDRKGKTSKRVHFVRSLIREVAGFAPYEKRITELLKVGKDKRALKVAKRKLGTHKRAKKKREEMSSVLRKMRAGGGAEKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CKF0</accession>
    <name>A0AAD5CKF0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Lipoyl-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_030667</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008061">
        <property type="protein sequence ID" value="KAI7742174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CKF0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004485">
        <property type="term" value="F:methylcrotonoyl-CoA carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06850">
        <property type="entry name" value="biotinyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000003">
        <property type="entry name" value="Acetyl-CoA carboxylase biotin carboxyl carrier protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001882">
        <property type="entry name" value="Biotin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050856">
        <property type="entry name" value="Biotin_carboxylase_complex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18866">
        <property type="entry name" value="CARBOXYLASE:PYRUVATE/ACETYL-COA/PROPIONYL-COA CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18866:SF33">
        <property type="entry name" value="METHYLCROTONOYL-COA CARBOXYLASE SUBUNIT ALPHA, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00188">
        <property type="entry name" value="BIOTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0092">Biotin</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="2">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7742174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="77" mass="8376" checksum="29A73D481F3C2098" modified="2024-05-29" version="1" fragment="single">MSGLVVKILVKDGMKVEEGQPMLVMEAMKMEHVIKAPSSGIVSGLQVALGQQVSDNSVLFNVKNIRFVDTVTRLGYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CM73</accession>
    <name>A0AAD5CM73_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cytochrome b5 heme-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_006645</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the fatty acid desaturase type 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007789">
        <property type="protein sequence ID" value="KAI7743164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CM73"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016717">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03506">
        <property type="entry name" value="Delta6-FADS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005804">
        <property type="entry name" value="FA_desaturase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012171">
        <property type="entry name" value="Fatty_acid_desaturase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19353:SF30">
        <property type="entry name" value="DELTA 8-(E)-SPHINGOLIPID DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19353">
        <property type="entry name" value="FATTY ACID DESATURASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00487">
        <property type="entry name" value="FA_desaturase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015921">
        <property type="entry name" value="FA_sphinglp_des"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0349">Heme</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="111"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="137"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="253"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="285"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="13">
        <location>
            <begin position="5"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7743164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="447" mass="51364" checksum="B7B52F5F2892F9A0" modified="2024-05-29" version="1">MGEPKKYITSDELKTHNQSGDLWICIHGKVYDVSEWMKTHPGGDLPLLNLAGQDVTDAYIALHPPTAYNHLHNFFNGYYLKDYTVSEVSKDYRKLYTEFNKMGLFEKKGHGVLISMIFIAMMFAVCVYGVLCSQSCLVHLVCGGLMGFLWIQSGWLGHDSGHYQIMIDRKFNRYAQILTGNCLAGISIGWWKRNHTAHHIAVNSLEYDPDLQHMPVFVVSSKFFDSLTSKYYERKLNFDWVTRLLVSYQHYTYYPVMCVARLNLFAQSFVLLLSSKPVANRGQELFGLLVFWIWYPLLVSCLPNWGERVMFVLASFSVTGIQHVQFTLNHFSSSVYVGEPRGNDWFEKQTNGTLNITCSEFMDWFHGGLQFQIEHHLFPRLPRCHLRKISPFVKDLCKKHGLPYDSASFYKANEMTVATLRAAALQARDLSKPVPKNMVWEALNSPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CMX7</accession>
    <name>A0AAD5CMX7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF7906 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_015990</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007496">
        <property type="protein sequence ID" value="KAI7744577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CMX7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057228">
        <property type="entry name" value="DUF7906"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31515:SF2">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31515">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25483">
        <property type="entry name" value="DUF7906"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="575"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7906" evidence="3">
        <location>
            <begin position="45"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="163"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7744577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="604" mass="68518" checksum="D6295F43B997975A" modified="2024-05-29" version="1">MIEWYDGFLNALNNVERLSEKQDVADIIQAKVVQLLNGKNEDMKLQWAKDLKSGDLSGVHAECLTDTWIGKDRWAFIDLSAGPFSWGPAVGGEGVRTELTLPNVEKTLGAVAEITEEEAEDHLQDAIQEKFAAFGDNDHQAIDILLAEIDIYELFAFKHCWGRRTKLSLCEELEERMRDLKTELESFETDDSVESHKQKAVDALKRMENWNLFSDISYEHSHNYTVARDTFLAHMGAVLWGSLRHIISPSIADGAFHHYEKISFQLFFITQESLPVDINAIKAGLSSLLLPSQNPTFSQHMLPLSEDPALAMAFSVARRASAHQLQRLSDHGSLKGSHAQSRSTLEVPIFWFIHGEPLLVDKHYQAKALSDMVIVVQSDSSSWESHLQCNGQSLLWNLRRPIKAALAAVSEHIAGLIPLHLVYSQAHETAIEDWLWSVGCSPLSVTSQGWHISQFISDTIARSYMLTTLEESIQQVNSAIHRLLAERLYMIIAELELYWQVEVAHERHLVDKYNYVVNRWKRISTVAGELRYVDALRLLSSLEDASNGFVEYVNTTVASLHQIHCTKQRKVKVEFDLTTIPAFLVVFFVLWLVLKPRRPKAKIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CPN8</accession>
    <name>A0AAD5CPN8_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_010388</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007525">
        <property type="protein sequence ID" value="KAI7744484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CPN8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd11660">
        <property type="entry name" value="SANT_TRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47206:SF7">
        <property type="entry name" value="HOMEODOMAIN-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47206">
        <property type="entry name" value="HOMEODOMAIN-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb-like" evidence="4">
        <location>
            <begin position="208"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="5">
        <location>
            <begin position="209"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="192"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="390"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="411"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7744484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="473" mass="50919" checksum="6FAAC4F8D8D37916" modified="2024-05-29" version="1">MNQDLKMSNKSNKCVITEEDVSTILQRYTATTVITLLQEVGQVQDAKIDWNALVKRTKTGITNPREYQMLWRHLAYRDELVEKLEDQAKPLASLIYDDDSDLEYELEAFPPVNNEASMEAAACVKVLLASGSTRDSGLDKGLTIEAPLMINIPNGKLTGDLSESQQVGGNIRGTNIAVPVCVQKQPLPAVASSTEELDKTNVGNLPLPPRRKRKPWTAAEDMELFAAVQKCGEGNWANILKGDFKGDRTASQLSQRWNIIKKRKNGNPNAKAGSQLSEAQLAARRALNMALDQPGIDGLKASSSLGRTKSSISTMIQNQSGEDPSALTKTYPKILPKTTPRPLVNGPDPVKAAAVAAGARIATPSAAAAILKQQLKTAIHIKTNVSSFRDLHSPNASRPHDHVGPVVPLNPVSTAPQETSGVTVSSSAEKAQQGQAAGFQDTSEKEVKENVGSSVEASVIMDTCENESAKQEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CQ54</accession>
    <name>A0AAD5CQ54_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_003739</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007049">
        <property type="protein sequence ID" value="KAI7746063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CQ54"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000180">
        <property type="entry name" value="serine/threonine-protein kinase STY46-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF223">
        <property type="entry name" value="INTEGRIN-LINKED PROTEIN KINASE FAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000654">
        <property type="entry name" value="Integrin-linked_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="54"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="114"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7746063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="394" mass="45404" checksum="4EBACCACC5BE0123" modified="2024-05-29" version="1">MAPERVSGSESEDEEVDGSDLIDPRVRLMFFSSDLDGIKELLDSGTDVNFKDIDNRTALHVAACQGFCDVAELLLNGGADVDPKDRWGSTMAPMHVKNAREVPEYEIDAKELDFTESVDITKGTFTIASWRGTKVAVKKLGDELFTDKEKVRAFRDELELLQKIRHPNVVQFLGAVTQSSPMMIVTEYLPKGDLRLFLKRKGSLKPITALKFAMDIARNILRADSGHIKVADFGISELMKVTKTDKHEKLMSYHDTTWRYVAPEVFRNEDYDTKVDVFSFALILQEMIEGNQPFFDKKEDDVPKAYVSKERPPFSAPSKSYAHGLKELIEECWHENPAERPTFRKIISRLESIYNSFNRRRRWKVRPLKWFQKMETIWKKEDSVRSSRDRSSYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CQX0</accession>
    <name>A0AAD5CQX0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NADP-dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_019169</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007332">
        <property type="protein sequence ID" value="KAI7745175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CQX0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43147:SF2">
        <property type="entry name" value="NADP-DEPENDENT OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43147">
        <property type="entry name" value="PROTEIN TAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="1">
        <location>
            <begin position="11"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7745175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="239" mass="26886" checksum="F98469B0620DFE4B" modified="2024-05-29" version="1" fragment="single">VSGPSGQMTWIRGGPTSLDRRNIKEAIDNSLLRAQTDYIDLYQVHWPDRYVPMFGETEYDPARQYNHVSFEEQLDALGRAVDAGKIRFIGVSNETPYGVMKFLQAAENNPAYPRIISVQNAYNLLCRNFDSGLAECCHLERVRLLAYSPLAMGLLSGKYSEGESRYNLSNHTIREATKAYISIAERYGIHPVSLAIAFVLRHPVVSSAIFGATKLWQLEEVVKACEVKLDPEIIAEIDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CS27</accession>
    <name>A0AAD5CS27_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">isocitrate dehydrogenase (NADP(+))</fullName>
            <ecNumber evidence="4">1.1.1.42</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_008836</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.42" evidence="4"/>
    <dbReference type="EMBL" id="JAMZMK010007112">
        <property type="protein sequence ID" value="KAI7745834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CS27"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004450">
        <property type="term" value="F:isocitrate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006102">
        <property type="term" value="P:isocitrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006739">
        <property type="term" value="P:NADP+ metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.718.10">
        <property type="entry name" value="Isopropylmalate Dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019818">
        <property type="entry name" value="IsoCit/isopropylmalate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004790">
        <property type="entry name" value="Isocitrate_DH_NADP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00127">
        <property type="entry name" value="nadp_idh_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006156">
        <property type="entry name" value="PRK08299.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11822:SF43">
        <property type="entry name" value="ISOCITRATE DEHYDROGENASE [NADP]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11822">
        <property type="entry name" value="NADP-SPECIFIC ISOCITRATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53659">
        <property type="entry name" value="Isocitrate/Isopropylmalate dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00470">
        <property type="entry name" value="IDH_IMDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0464">Manganese</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0521">NADP</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Isopropylmalate dehydrogenase-like" evidence="11">
        <location>
            <begin position="1"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7745834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="230" mass="25805" checksum="D6C415C2D3A67F44" modified="2024-05-29" version="1">MYNTDESITAFAEASMNTAYLKKWPLYLSTKNTILKKYDGRFKDIFQEVYEKNWKSKFEAAGIWYEHRLIDDMVAYALKSDGGYVWACKNYDGDVQSDFLAQGFGSLGLMTSVLVCPDGKTIEAEAAHGTVTRHYRVHQKGGETSTNSIASIFAWTRGLAHRAKLDDNAKLLDFTEKLEAACIGCVESGKMTKDLALIIHGSKLSREHYLNTEEFIDAVADELKARLSSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CSI9</accession>
    <name>A0AAD5CSI9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Probable cytosolic iron-sulfur protein assembly protein CIAO1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_016728</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Essential component of the cytosolic iron-sulfur (Fe/S) protein assembly machinery. Required for the maturation of extramitochondrial Fe/S proteins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the WD repeat CIA1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006739">
        <property type="protein sequence ID" value="KAI7747526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CSI9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03037">
        <property type="entry name" value="ciao1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028608">
        <property type="entry name" value="CIAO1/Cia1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="17"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="117"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="162"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="212"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="295"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7747526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="333" mass="36669" checksum="25487E623E46D325" modified="2024-05-29" version="1">MTSIEEEGLQLKELQTLTGHTDRVWGLAWNPVTGTAGHPSMLASCGGDKTVRIWQQQQQTPNSTSFHSLEVLEEIHTRTVRSCVWSPSGEKLATASFDATTAILEQNGNGFECVSTLEGHENEVKSVSWNASGSLLATCSRDKCVWIWEVLPGNEFDCVSVLQGHTQDVKMVQWHPTVDVLFSCSYDNTIKVWAEDGDSDDWRCVQTLNESNCGHSSTVWAISFNATGEKMVTCSDDLTIKVWGVDIARLQSGDTNASWEGIIATGAADDAICLFIESEDHSVDGPKYKLLLKKEKAHDMDINAVRWSPTENRLLASASDDGTIKIWKLDSIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CSN9</accession>
    <name>A0AAD5CSN9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">histone acetyltransferase</fullName>
            <ecNumber evidence="3">2.3.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">M8C21_005523</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">AA19_3_7</strain>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Acetyltransferase enzyme. Acetylates histones, giving a specific tag for transcriptional activation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-lysyl-[protein] + acetyl-CoA = N(6)-acetyl-L-lysyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45948"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010007092">
        <property type="protein sequence ID" value="KAI7745895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CSN9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000123">
        <property type="term" value="C:histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004402">
        <property type="term" value="F:histone acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.90:FF:000018">
        <property type="entry name" value="Histone acetyltransferase HAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.90:FF:000022">
        <property type="entry name" value="Histone acetyltransferase of the CBP family 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.90">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1020.10">
        <property type="entry name" value="TAZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031162">
        <property type="entry name" value="CBP_P300_HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013178">
        <property type="entry name" value="Histone_AcTrfase_Rtt109/CBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035898">
        <property type="entry name" value="TAZ_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000197">
        <property type="entry name" value="Znf_TAZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000433">
        <property type="entry name" value="Znf_ZZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043145">
        <property type="entry name" value="Znf_ZZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13808">
        <property type="entry name" value="CBP/P300-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13808:SF1">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02135">
        <property type="entry name" value="zf-TAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00569">
        <property type="entry name" value="ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01250">
        <property type="entry name" value="KAT11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00551">
        <property type="entry name" value="ZnF_TAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00291">
        <property type="entry name" value="ZnF_ZZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57933">
        <property type="entry name" value="TAZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51727">
        <property type="entry name" value="CBP_P300_HAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01357">
        <property type="entry name" value="ZF_ZZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50135">
        <property type="entry name" value="ZF_ZZ_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0010">Activator</keyword>
    <keyword evidence="13" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CBP/p300-type HAT" evidence="18">
        <location>
            <begin position="1"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="ZZ-type" evidence="17">
        <location>
            <begin position="148"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="ZZ-type" evidence="17">
        <location>
            <begin position="268"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="95"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="96"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="19">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAI7745895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="385" mass="44439" checksum="713BF660AFA8FCC8" modified="2024-05-29" version="1" fragment="single">PPLKGEDYILYCHPEIQKTPRSDKLREWYLSMLRKASKENIVVGLTNLYDHFFVSSGECKAKVTAARLPYFDGDYWPGAAEDMIYQIRQEEEGRKQNKKGLVKKSMTKRALKASGQTDLSGNASKDLMLMRRLGESISPMKEDFIMVHLQHACTHCCILMVSGNRWVCHQCKKFQICDKCYEIEQKLEDRERHPISHREKHPLYPVEINDVPADTTDKDDILESEFFDTRQAFLSLCQGNHYQYDTLRRAKHSSMMVLYHLHNPTAPAFVTTCNRCHNDIETGQGWRCDVCPDYDVCNSCYHKDGGIDHPHKLTPHVSIAERDAQNKEARQQRVVQLRKMLDLLVHASQCRSPLCQYPNCRKVKGLFRHGISCKTRASGGCLFQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CTJ0</accession>
    <name>A0AAD5CTJ0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_000436</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007014">
        <property type="protein sequence ID" value="KAI7746271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CTJ0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7746271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="87" mass="10505" checksum="267055F3D1EEC358" modified="2024-05-29" version="1" fragment="single">MDSLGGVVIWFEETGSQFWIRRWIASLRFPRVFMVFQLRVNFEKDEKKDNNSSQAIWERQHDYAANKSRFKRPTETKGSKRDMFNNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CTK2</accession>
    <name>A0AAD5CTK2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glycosyl transferase family 1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_032537</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006713">
        <property type="protein sequence ID" value="KAI7747644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CTK2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46635">
        <property type="entry name" value="GLYCOSYL TRANSFERASE FAMILY 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46635:SF1">
        <property type="entry name" value="GLYCOSYL TRANSFERASE FAMILY 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="3">
        <location>
            <begin position="124"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="362"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="364"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7747644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="504" mass="57099" checksum="2D53F0B3C962D172" modified="2024-05-29" version="1" fragment="single">KYASDDQQQLIDAWKTVFHRATVVVFPNHVLPMYYAAFDAGNYFVIPGFPSKACKLDNSMIVFEENLRANMNFGAHDLVIAITGSQFLYKGLWVEHALVLQALSPLLAESNADDNLSQRLRIIFLSQDLSGNYSAAIEEIASNLNYPNGTVNHAAINDDNDAYKILSIADLVIYGSFLEEQSFPEILVKAMCFEKPIIAPDLAVIKKYVDDKVNGFLFPKRNIKIVTQIMPQLVSKGKLSPLARNIASIGKHTAKNMMVLESVEGYASLIENVVNLPSEVASPRAVSDIPSNFKTEWQWHYFDDITDGKYVNRSLRIHHFLNKLENRSNRTLKASFADISADDTFLYSIWEEEKSIQTARVKKRREDGELRDRSDQPRGTWEEVYRSAKKADRNKNDLHERDDGELERTGQPLCIYEPYFGQGAWPFLHSDSLYRGLGLSSKGRRSRTDDVDAPSRLPLLSIPYYRDALGDFGALFAIANHIDRIHKNSWIGFSSWRATAKKAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CUI8</accession>
    <name>A0AAD5CUI8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_019116</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006718">
        <property type="protein sequence ID" value="KAI7747600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CUI8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="18"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="60"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7747600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="274" mass="31508" checksum="30212A989BE3657D" modified="2024-05-29" version="1">MEELSKIAMAYYQASTDHLQTLARDFFSAMDHDGDGKIDLREFLEFMKEEGCHQMTNPFMFNQLDLDRNGTLDFVEVMTLYYIIKSGRPFCDHCRKFITSTYLTCVGCLEDPNDRSFYLCLDCYSRQVCDHTHNDMSRFLDNYSFLEAMTKLKIGELRSRPSETDSVMIPDKPHSANHNHTWNPSLAMGQVPQRPAPVHNQYWGSQWAPHSSVAPSIHNHTWIQNNYSYNHTPHYHVQQPAAIVPQRQQWQAALGALNAALHISAIGSNFCSIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CV68</accession>
    <name>A0AAD5CV68_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein EFFECTOR OF TRANSCRIPTION 2-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_032814</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006451">
        <property type="protein sequence ID" value="KAI7748893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CV68"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038909">
        <property type="entry name" value="Effector_transcript"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35133">
        <property type="entry name" value="PROTEIN EFFECTOR OF TRANSCRIPTION 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35133:SF1">
        <property type="entry name" value="PROTEIN EFFECTOR OF TRANSCRIPTION 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19239">
        <property type="entry name" value="GIY_YIG_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7748893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="384" mass="43178" checksum="61F7A39A0955FA8C" modified="2024-05-29" version="1">MVNTVNTAVTAVTAAPSLKRENCTRTKHDSVFSDWKILIGACDWEDYLLNKEGAERYRTHNLPNCSSCPGLYELGIAVSVTRKKGKIISKLNSKNVIPVYLGQADNVRTRLQQYGRDGAHLENGWSNGEQNDRKVLGLFSDIFSYGFAIAFRWAPMESKKDAEKTESQLLKTFDYAWNRGMNGERRPNDIHKKLNNTGLHRKRVHLVFNKIRVFPPKEVGLRIKHCDPPVLENGSSFYTKQNDPSAFVRIFKFGRSRPTLVSKDGNMNNDNVSTCGVALGHGSICIRPPVEGRKRCSEHKGMKVNAYIKPRLDLESGTLCNSQKGCEEVKHEGMMVNEKKSKIPGTDSLTCVAITLNGSCCRRKPSKDSKFCWQHAAIRTNINN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CVP0</accession>
    <name>A0AAD5CVP0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Sphingosine-1-phosphate lyase</fullName>
            <ecNumber evidence="15">4.1.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Sphingosine-1-phosphate aldolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">M8C21_028218</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">AA19_3_7</strain>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 18 19">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass type III membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the group II decarboxylase family. Sphingosine-1-phosphate lyase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.27" evidence="15"/>
    <dbReference type="EMBL" id="JAMZMK010006396">
        <property type="protein sequence ID" value="KAI7749163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CVP0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008117">
        <property type="term" value="F:sphinganine-1-phosphate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030149">
        <property type="term" value="P:sphingolipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000020">
        <property type="entry name" value="sphingosine-1-phosphate lyase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000020">
        <property type="entry name" value="Sphingosine-1-phosphate lyase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="6.10.140.2150:FF:000001">
        <property type="entry name" value="Sphingosine-1-phosphate lyase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050477">
        <property type="entry name" value="GrpII_AminoAcid_Decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002129">
        <property type="entry name" value="PyrdxlP-dep_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42735">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42735:SF6">
        <property type="entry name" value="SPHINGOSINE-1-PHOSPHATE LYASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00282">
        <property type="entry name" value="Pyridoxal_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13 19" id="KW-0456">Lyase</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 18 19" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="8" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="18">
        <location>
            <position position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00038302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00038965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00042568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00067991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR602129-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAI7749163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="569" mass="62134" checksum="E118FED5615387EF" modified="2024-05-29" version="1">MESSIIPTLIQLRASANSFLSQYEPLAIVFVPIVSFLIARVLQSFIQLVQEKGVKPTVIGFVMTLVKLVPGVKKYMDAEKQKTAAYCHGLVLGSSRTCHVDATYDYEVTSEVVDKLQSSGKSSRDGWKTELPNTGLGTRVLEEMKDIKQKDPAWQGKCSGTVYIGGNESEGHFSLINEACSMFAHTNPLHLDVFKSVVRFEAEVVAMTGALFGSKEKASGGKICGNMTSGGTESILLAVKSSRDYMRAKKNITCPEMIIPESAHSAYDKACQYFKIKLWRVPVNKEFQADVKAIKKYINKNTIMIVGSAPGFPHGIVDPIEELGELAVSYGICLHVDLCLGGFVLPFARKLGYPIPPFDFTVQGVTSISADVHKYGLAPKGTSIVLYRNHDIRKVKSQMVTEWSGGLYVSPTIAGSRPGNAIAGAWAAMISLGQEGYLEHTREIMEASKRIQQGAKEIPELFIVGRPDMTIVAFGSNVIDIFEVNDVLSSKGWHLNPLQRPNSIHICVTLQHVPVVDEFLNDLKDSVRIVKKNPGPISGGLAPIYGAAGKIPDRGMVSELLVDYMDNAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CWV0</accession>
    <name>A0AAD5CWV0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ArsA/GET3 Anion-transporting ATPase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_027503</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the arsA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006268">
        <property type="protein sequence ID" value="KAI7749838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CWV0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043529">
        <property type="term" value="C:GET complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071816">
        <property type="term" value="P:tail-anchored membrane protein insertion into ER membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02035">
        <property type="entry name" value="ArsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000936">
        <property type="entry name" value="Arsenical pump-driving ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025723">
        <property type="entry name" value="ArsA/GET3_ATPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016300">
        <property type="entry name" value="ATPase_ArsA/GET3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00345">
        <property type="entry name" value="GET3_arsA_TRC40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10803">
        <property type="entry name" value="ARSENICAL PUMP-DRIVING ATPASE ARSENITE-TRANSLOCATING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10803:SF0">
        <property type="entry name" value="ATPASE GET3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02374">
        <property type="entry name" value="ArsA_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="ArsA/GET3 Anion-transporting ATPase-like" evidence="8">
        <location>
            <begin position="89"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="279"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7749838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="412" mass="45175" checksum="C405EB085B8D128B" modified="2024-05-29" version="1">MAITSYHHHATTFSSPLSPNRLTSVPRTAFISYPTPFNPYKLHSIFSANVLSLSSNKKQSKKLFTVRSAVAPVESTAEFDEMVSGTQRKYYMLGGKGGVGKTSCAASLAVKFANNGHPTLVVSTDPAHSLSDSFAQDLTGGTLVPVEGPLSPLFALEINPEKAREEFRDVSKKNGGTGVKDFMDGMGLGMLADQLGELKLGELLDTPPPGLDEAIAISKVIQFLESQEYNMFTRIVFDTAPTGHTLRLLSLPDFLDASIGKILKLRQKISSATSAIKNVFGQEETRQDASDKLEKLRERMIKVRELFRDTNSTEFVIVTIPTVMAISESSRLRASLTKENVPVKRLIVNQILPPSASDCKFCAMKRKDQTRALDTIRNDPELSSLLLVQAPLVDVEIRGVPALQFLGDMIWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CZ67</accession>
    <name>A0AAD5CZ67_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_030509</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the DESIGUAL family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006414">
        <property type="protein sequence ID" value="KAI7749060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CZ67"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009606">
        <property type="entry name" value="DEAL/Modifying_wall_lignin1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052222">
        <property type="entry name" value="DESIGUAL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31769">
        <property type="entry name" value="OS07G0462200 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06749">
        <property type="entry name" value="DUF1218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="49"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="94"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="137"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="174"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7749060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="196" mass="20741" checksum="1957506C64905338" modified="2024-05-29" version="1">MDNKLLGFCAIAAVLGIISAVFGFAAEATRVKASEVEIIYNTCVYPSSPALALAIVASAFTIITQIYLTSIYLGSGCCINDPSVPKVSGVLCKFSWFASFAAVVTFLVAGAQNNRRSREGRRVDLYHIKCFAVKPGFFSVGAAVALLSVIFGIAAFLTIIPSPQTNTNPAVELPLGANVGPERNPRPFPSPQHASR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CZU6</accession>
    <name>A0AAD5CZU6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="11">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">M8C21_004207</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">AA19_3_7</strain>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the helicase family. RecQ subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="11"/>
    <dbReference type="EMBL" id="JAMZMK010006021">
        <property type="protein sequence ID" value="KAI7751004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CZU6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18794">
        <property type="entry name" value="SF2_C_RecQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001334">
        <property type="entry name" value="ATP-dependent DNA helicase Q-like 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000772">
        <property type="entry name" value="ATP-dependent DNA helicase Q4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004589">
        <property type="entry name" value="DNA_helicase_ATP-dep_RecQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00614">
        <property type="entry name" value="recQ_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710:SF108">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE Q4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710">
        <property type="entry name" value="DNA HELICASE RECQ FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="14">
        <location>
            <begin position="348"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="15">
        <location>
            <begin position="512"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="258"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="18"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="267"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="281"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAI7751004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="950" mass="105852" checksum="2EEBE73DB7571C41" modified="2024-05-29" version="1">MEHSDSDSDGSHISSTPPRQPQPPPAPRTLLFLDDKRRKSKGTCSRPILKIKPSSKRSKPPPPISQPPIHQSTSHHLPFQLRQSSGCDLSDIPTPTSSNSNLNLPFQIRRLSSSSSFDSTDILPAGGTCLSRFASFSKLRNSSFNFNSPEKGIDLVNVNCSQRVAKKHPNLIGSCGSTLEQTKKLDSDTLNETLGVVDVTSSNLGKVVRKHPNLIRSCIIPTAQVTKPKLVNEGNFVRLNINGHGGRKRFAHKVRKRNPNAYNGNRKSYKRSKRKLKNAGEGEEDENGFCDEGWPMEENERTETRLDSDVIEKAVFDVRRDPSDDNLIKLLKLVYGYDSFRDGQLEAIKLILSGKSSMVILPTGAGKSLCYQLPATVLEGLTLVISPLVALMYDQLKQLPPVIPGGLLCSSQVLFVSPERLLNTEFTSIFSNLLISLVVIDEAHCISEWSHNFRPSYMRLRASLLRSSLGVDCILAMTATATAKTMRDVMCSLEIPSTNLVQAAQIRDNLQLMKDLMALLKASPYTEIKSIIIYCKYQSETDMISKFLCDSNIRAKSYHSGIPAKDRRRTQELFCSNKIRVIVATVAFGMGLDKSDVGAVIHYSLPESLEEYVQEIGRAGRDGRVSYCHLFFDDTTYFKLRSLMHSDGQDQYTVNKFLSQVFSGDSHSQGRICTIVKESASRKFDMKEEVILTILTRLELAEEQYLRLLPQTSVTCVLNFHMTSPALLAAKDTVIATVLEKSEVKDGQYVFEIPTVACSMGIQASTVTNHLQNLKLKGEITYELKDPAYCYMIVNLPKDICSLAADLAKWLSEVESCKVCRVDAMFNAALYAVKTCDRTNGCTDSQHTPCLQKKILDYFNADSDNNVPNKMAQTSRFLRADIKVFLQSNSHAKFTPRAIARILHGIPSPAFPSSTWCRTHFWGRYIQTNFDIIMEAAKAELMDCAGKSAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D0F9</accession>
    <name>A0AAD5D0F9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Leucine-rich repeat-containing N-terminal plant-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_028940</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RLP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006150">
        <property type="protein sequence ID" value="KAI7750469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D0F9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000041">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000095">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046956">
        <property type="entry name" value="RLP23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063">
        <property type="entry name" value="LRR RECEPTOR-LIKE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48063:SF112">
        <property type="entry name" value="RECEPTOR LIKE PROTEIN 30-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="11">
        <location>
            <begin position="22"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="12">
        <location>
            <begin position="408"/>
            <end position="606"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7750469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="819" mass="91432" checksum="1AE43312942D97B0" modified="2024-05-29" version="1">MFLVVTKYACLGALNSTRSCHEPERLALLEFKHSVKDKYEMLSSWVGSDCCSWKGVRCDGATGSVVSLLLKGNYYSYGRDYLKEEYYLVGDKVNASLAELRHLQHLDLSSNNFKGSQIPKFIGSFKQLSYLNLSNACFNGIIPHHIGNLSNLKVLDLASFPYSDYMLSSNDIAWVSRLSSLEHLDLSSVNLSQSKNVDMVFYMIPSLTYLSLQLCELSNVDLHLHLNSSKILPNIEHLDLSSNLFQGQLPHFFQNKTVLTFLDLSGYDLSLAQISVNQLIMIPSLSELHLSGCGLHSALFSPTYFNSSAHSHIQYLDLSANSIKGRIPYGLMNITSLKVLDLSSNSLNLSIPVIPNLLKLDVSYNKFEHVELVGIWRQCHLKKLRLSENYFGGEMWGPSSNTSECSHYALEVLELYDNKLNGPIPESIGKLNNLRVLILSSNQFTGSIPKALERLRSLEVLVLSKNGLTGPVPSFLGKLTKLDLSYNQLSGSIPESLGRLTGLTLLRLASNLLTGPIPVSIGQLNKLTGLVVSNNSLEGVVTEAHFANLSMLKLFNTFANHKLIFNLSNEWMPPFQLKYVKLSSCKIVNGFPQWLQNRRKLDTLVLSNASLYGLLPTWLRNMSMLYTLDISHNKLTGSLINLPFSEYTMYLLLQDNLFKGSIPRSVCKTTGLGILDVSSNRLSGNIPDCLENLQYIQMLILNSNGLSGVLPSSLGNNHSSLKWLKLNDNNFNGQIPLDLRNLTSLEVLDLGNNDFSGNIPKWIGEKLSALRIFRLHKNNFIGSIPHSLCKCSSLQILDVSTQQLNRINPSLCWRVDRDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D109</accession>
    <name>A0AAD5D109_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ATP-dependent Clp protease proteolytic subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_013056</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec, and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs).</text>
            <dbReference type="EC" id="3.4.21.92"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the peptidase S14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006093">
        <property type="protein sequence ID" value="KAI7750754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D109"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009368">
        <property type="term" value="C:endopeptidase Clp complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009532">
        <property type="term" value="C:plastid stroma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051117">
        <property type="term" value="F:ATPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07017">
        <property type="entry name" value="S14_ClpP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001907">
        <property type="entry name" value="ClpP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023562">
        <property type="entry name" value="ClpP/TepA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018215">
        <property type="entry name" value="ClpP_Ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10381">
        <property type="entry name" value="ATP-DEPENDENT CLP PROTEASE PROTEOLYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10381:SF12">
        <property type="entry name" value="ATP-DEPENDENT CLP PROTEASE PROTEOLYTIC SUBUNIT 5, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00574">
        <property type="entry name" value="CLP_protease"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00127">
        <property type="entry name" value="CLPPROTEASEP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00381">
        <property type="entry name" value="CLP_PROTEASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0720">Serine protease</keyword>
    <feature type="active site" evidence="5">
        <location>
            <position position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7750754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="332" mass="36513" checksum="9CE5EDEFFAC0DAA9" modified="2024-05-29" version="1">MAQTCLPSLRLNSTVFSSTESPNSIPQHTFLPLPSRKVGKLRSNQKTLRTSSVKAVYSGDYSTLDKKSRPGLWSIREDIQVPSSPYFPTYAQGQGPPPMMQERFQSVISQLFQYRIIRCGGAVDDDMANIIVAQLLYLDAVDPTKDIVMYVNSPGGSVTAVLETLNVKNQLSSVTVYGILLGMAIFDTMKHIRPDVSTVCVGLAASMGAFLLGAGTKGKSANEMLHHKANLNGYLAYHTGQSLDRINQDTDRDYFMSAKEAKEYGLIDGVIMNPLKALQPLPAAYVTFFSIAWLQIDLQWSHAYVGAPCRLFIHARRKVLGLICDSVKKASP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D1L1</accession>
    <name>A0AAD5D1L1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Clp R domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_020338</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the ClpA/ClpB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005683">
        <property type="protein sequence ID" value="KAI7752446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D1L1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19499">
        <property type="entry name" value="RecA-like_ClpB_Hsp104-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000025">
        <property type="entry name" value="ATP-dependent Clp protease subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000010">
        <property type="entry name" value="Chaperone clpB 1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1780.10:FF:000006">
        <property type="entry name" value="Chaperone protein ClpB3, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1780.10">
        <property type="entry name" value="Clp, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019489">
        <property type="entry name" value="Clp_ATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036628">
        <property type="entry name" value="Clp_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004176">
        <property type="entry name" value="Clp_R_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001270">
        <property type="entry name" value="ClpA/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018368">
        <property type="entry name" value="ClpA/B_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028299">
        <property type="entry name" value="ClpA/B_CS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041546">
        <property type="entry name" value="ClpA/ClpB_AAA_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050130">
        <property type="entry name" value="ClpA_ClpB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11638">
        <property type="entry name" value="ATP-DEPENDENT CLP PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11638:SF18">
        <property type="entry name" value="HEAT SHOCK PROTEIN 104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07724">
        <property type="entry name" value="AAA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17871">
        <property type="entry name" value="AAA_lid_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02861">
        <property type="entry name" value="Clp_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10431">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00300">
        <property type="entry name" value="CLPPROTEASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01086">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81923">
        <property type="entry name" value="Double Clp-N motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51903">
        <property type="entry name" value="CLP_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00870">
        <property type="entry name" value="CLPAB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00871">
        <property type="entry name" value="CLPAB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Clp R" evidence="9">
        <location>
            <begin position="76"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="483"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7752446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="902" mass="101170" checksum="1EDBEB4B28E19CD5" modified="2024-05-29" version="1" fragment="single">MSTMSTLFHLPSSICTDRTITSSFAQPSVSVNSFSKPRRVSLRSLKLKTRTNDVLLRKSSRSLVVRCDASSSGRITQQEFTEMAWQAIVSSPEVAKENKHQIVETEHLMKALLEQKNGLARRIFSKAGVDNTRLLEATDKFIQRQPKVIGESAGSMLGRDLESLMQRARDYKKEYGDSFVSVEHLVLGFVQDNRFGKQLFKEFQISLKTLKSAIESIRGRQTVIDQDPEGKYESLEKYGKDLTAMAREGKLDPVIGRDDEIRRCIQILSRRTKNNPVLIGEPGVGKTAISEGLAQRIVQGDVPQALMNRKLISLDMGALIAGAKYRGEFEDRLKAVLKEVTESDGQIILFIDEIHTVVGAGATNGAMDAGNLLKPMLGRGELRCIGATTLDEYHTVSILRGLRERYELHHGVRISDSALVEAAILSDRYISGRFLPDKAIDLVDEAAAKLKMEITSKPTALDEINRAVLKLEMERLSLTSDTDKASKDRLNRLETELALLKERQAELTEQWEHEKSVMTNIQSIKEEIDRVNLEIQQAERKSMLREEVTGDDIAEIVSKWTGIPLSKLKQSEREKLLYLENELHKRVVGQDPAVTSVAEAIQRSRAGLSDPHRPIASFMFMGPTGVGKTELAKALASYLFNTEESLVRIDMSEYMEKHAVSRLIGAPPGYVGYEEGGQLTEIVRRRPYAVILFDEIEKAHADVFNVFLQILDDGRVTDSQGRTVSFTNTVIIMTSNVGSQYILDTDDDSAPKEQAYETIKRRVMDAARSVFRPEFMNRVDEYIVFQPLDRSQINRILERVQKRIADKKLKIEVSEAAVELLGSLGYDPNYGARPVKRVIQQYVENELAKGILRGEFKDEDTISVDTEVTAFSNGQLPQQKLVFKRVDSPSGDTAEGQEAVPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D3C1</accession>
    <name>A0AAD5D3C1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_014052</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005987">
        <property type="protein sequence ID" value="KAI7751085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D3C1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08267">
        <property type="entry name" value="MDR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052733">
        <property type="entry name" value="Chloroplast_QOR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44013">
        <property type="entry name" value="ZINC-TYPE ALCOHOL DEHYDROGENASE-LIKE PROTEIN C16A3.02C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44013:SF1">
        <property type="entry name" value="ZINC-TYPE ALCOHOL DEHYDROGENASE-LIKE PROTEIN C16A3.02C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13602">
        <property type="entry name" value="ADH_zinc_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="1">
        <location>
            <begin position="16"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7751085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="322" mass="34031" checksum="DA9B03AC889101B5" modified="2024-05-29" version="1">MGDKLMHALQYDSYGGGAAGLKHVEIPIPKPSKGELLLKLEATSINPVDWKIQKGMLRPLFPPTDVAGEVIDVGPGVNNFKVGDKVVSMLDVFKGGGLSEYAVGNQKLTVLRPPEVSAAEGAGLPVAGLTALQALTYHGGITLDKTSPTTNVLITAASGGVGQYAVQLAKLGNVHVTATCGERNIDLVKGLGADEVIDYKTPQGAALKSPSDKKYDVVVHCATFIPWSTFEPNLGPNGKVIDITPNATAFWTAVVKKVTCSKKQLVPSLLFPKAKNLEYLVGLVKEGKLKTIIDSKYPLSKAEEAWAKSIDGHATGKIIVEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D3G1</accession>
    <name>A0AAD5D3G1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Chloroplastic lipocalin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_032428</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the calycin superfamily. Lipocalin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005414">
        <property type="protein sequence ID" value="KAI7753503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D3G1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036094">
        <property type="term" value="F:small molecule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012674">
        <property type="entry name" value="Calycin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002345">
        <property type="entry name" value="Lipocalin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022272">
        <property type="entry name" value="Lipocalin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11430:SF32">
        <property type="entry name" value="CHLOROPLASTIC LIPOCALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11430">
        <property type="entry name" value="LIPOCALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50814">
        <property type="entry name" value="Lipocalins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00213">
        <property type="entry name" value="LIPOCALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7753503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="351" mass="39169" checksum="CB0B07F35ED14F72" modified="2024-05-29" version="1">MAATGNSPLLLVSSHSPPVSRKVPSFKCSIKSPLSFSTKDAAKHVVSGLAAAGFILLSPTHQVLATELSYNNLCQIASTTNNIPSLNLDEGSNLMMMQGMTARNFDPVRYSGRWFEVASLKRGFAGQGQEDCHCTQGVYTVDMKTPAIQVDTFCVHGGPDGYITGIRGRVQCLSEEETEKTETDLERTEMIKEKCYLRFPTLPFIPKEPYDVLDTDYDNFALVSGAKDKGFIQLVLIWDRIELIHLIPSPPCFKWMFLIYSRTPNPGTEFIEKYKAVLADFGYDASKIKDTPQDCEVSDSRLAAMMSMNGMQQALTNQFPDLELKSAVEFNPFTSVFDTLKKLVQLYFKQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D3N9</accession>
    <name>A0AAD5D3N9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_019624</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005631">
        <property type="protein sequence ID" value="KAI7752717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D3N9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01396">
        <property type="entry name" value="MeCP2_MBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.890.10">
        <property type="entry name" value="Methyl-cpg-binding Protein 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001739">
        <property type="entry name" value="Methyl_CpG_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011124">
        <property type="entry name" value="Znf_CW"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12396:SF0">
        <property type="entry name" value="METHYL-CPG BINDING DOMAIN PROTEIN-LIKE, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12396">
        <property type="entry name" value="METHYL-CPG BINDING PROTEIN, MBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01429">
        <property type="entry name" value="MBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07496">
        <property type="entry name" value="zf-CW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00391">
        <property type="entry name" value="MBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50982">
        <property type="entry name" value="MBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51050">
        <property type="entry name" value="ZF_CW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CW-type" evidence="11">
        <location>
            <begin position="93"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="MBD" evidence="10">
        <location>
            <begin position="158"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="13"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="255"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7752717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="304" mass="34309" checksum="CD0482125F05C716" modified="2024-05-29" version="1">MQPSVTELAIRVTGSHQDSVNGFDSPPKRKEQTNIVDNVIELSSSDSEDENTQSCNDNHQQLVLYDPSVNGVSPIEDFPEPISFKPPPVKRILPAVGAFTVQCANCFKWRFIPTKQKYEEIREHITERPFVCATAQEWRPDVSCEDPPDIEPDGSRLWAIDKPNIVQPPPGWQRSLRIRGEGSTKFADIYYTPPGGKVLRSIHDVEKYLIKHPEYVEQGVTLAQFSFQVPKPLQENYVRKRSSRVAALQDCSSTRMPESFEPFPEKALALPGPDPSMNLQIGMNPSPKKARSSPGNPRHDFDRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D4K0</accession>
    <name>A0AAD5D4K0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_008278</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005787">
        <property type="protein sequence ID" value="KAI7751956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D4K0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18919">
        <property type="entry name" value="bHLH_AtBPE_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000002">
        <property type="entry name" value="Basic helix-loop-helix transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024097">
        <property type="entry name" value="bHLH_ZIP_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12565:SF430">
        <property type="entry name" value="MYC-TYPE, BASIC HELIX-LOOP-HELIX (BHLH) DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12565">
        <property type="entry name" value="STEROL REGULATORY ELEMENT-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="6">
        <location>
            <begin position="254"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="155"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="173"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="207"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7751956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="410" mass="45226" checksum="455DBC7FD5D4282A" modified="2024-05-29" version="1">MQKENIFTNEGNGIPQAWNSIFGIGIQGNEGNGIPSLEWESNCVINSIDQRDIFESSLTSIISSPANSGTGIPNSGSRDNIILKELIGRLGSICNSSDISHITTSSCYNTPLNSPQKVVNMSITDHNIHGNGNHQFPLAKYSSLVGKNDYNNGMESGKLSRVSTETGIKGHGNNNNININNNGRKRKEVAKGKGNGKETQSSSTLFAKDDKVVAEVEKEESDAKRSKSNDGKKSNEKSTEPPKDYIHVRARRGQATDSHSLAERVRREKISERMKFLQDLVPGCNKVTGKAVMLDEIINYVQSLQRQVEMFRPQVVVPNNVDPFDTSVHSFPYAFHSQINSMILDRPENQFSMNPLVAAMHRNSTVKPSHIDGFGEASTLWENDLQSVVKMALGGQDQPQDFHGQMKVEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D4R4</accession>
    <name>A0AAD5D4R4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_011659</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005552">
        <property type="protein sequence ID" value="KAI7753090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D4R4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027728">
        <property type="entry name" value="Topless_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44083:SF5">
        <property type="entry name" value="PROTEIN TOPLESS-RELATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44083">
        <property type="entry name" value="TOPLESS-RELATED PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7753090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="135" mass="14794" checksum="E8235E2197DE869A" modified="2024-05-29" version="1" fragment="single">MSNDVNESKPAEESAACIALSKNDSYVMSASGGKVSLFNMMTFKVMTTFMAPPPAATYLAFHPQDNNIIAIGMEDSTIQIYNVRVDESSATLKLWWRVETARQCYVSRPMARLDSPKGALFGGRVIDGDSDFALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D5R7</accession>
    <name>A0AAD5D5R7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Transcription elongation factor SPT5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_033839</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SPT5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010002534">
        <property type="protein sequence ID" value="KAI7754771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D5R7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032044">
        <property type="term" value="C:DSIF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032784">
        <property type="term" value="P:regulation of DNA-templated transcription elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06081">
        <property type="entry name" value="KOW_Spt5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06082">
        <property type="entry name" value="KOW_Spt5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06083">
        <property type="entry name" value="KOW_Spt5_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06084">
        <property type="entry name" value="KOW_Spt5_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06086">
        <property type="entry name" value="KOW_Spt5_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09888">
        <property type="entry name" value="NGN_Euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000027">
        <property type="entry name" value="Transcription elongation factor SPT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000028">
        <property type="entry name" value="Transcription elongation factor SPT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.940:FF:000007">
        <property type="entry name" value="Transcription elongation factor SPT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.940">
        <property type="entry name" value="NusG, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005824">
        <property type="entry name" value="KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041973">
        <property type="entry name" value="KOW_Spt5_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041975">
        <property type="entry name" value="KOW_Spt5_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041976">
        <property type="entry name" value="KOW_Spt5_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041977">
        <property type="entry name" value="KOW_Spt5_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041978">
        <property type="entry name" value="KOW_Spt5_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057935">
        <property type="entry name" value="KOW_Spt5_6_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057934">
        <property type="entry name" value="KOW_Spt5_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057936">
        <property type="entry name" value="KOWx_Spt5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005100">
        <property type="entry name" value="NGN-domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006645">
        <property type="entry name" value="NGN-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036735">
        <property type="entry name" value="NGN_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039385">
        <property type="entry name" value="NGN_Euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005825">
        <property type="entry name" value="Ribosomal_uL24_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039659">
        <property type="entry name" value="SPT5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022581">
        <property type="entry name" value="Spt5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017071">
        <property type="entry name" value="TF_Spt5_eukaryote"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11125">
        <property type="entry name" value="SUPPRESSOR OF TY 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11125:SF7">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR SPT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23042">
        <property type="entry name" value="KOW1_SPT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23284">
        <property type="entry name" value="KOW2_Spt5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23291">
        <property type="entry name" value="KOW4_SPT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23290">
        <property type="entry name" value="KOW5_SPT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23038">
        <property type="entry name" value="KOW6_SPT51-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23287">
        <property type="entry name" value="KOW7_SPT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23037">
        <property type="entry name" value="KOWx_SPT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03439">
        <property type="entry name" value="Spt5-NGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11942">
        <property type="entry name" value="Spt5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036945">
        <property type="entry name" value="Spt5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00739">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00738">
        <property type="entry name" value="NGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01108">
        <property type="entry name" value="RIBOSOMAL_L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0010">Activator</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NusG-like N-terminal" evidence="11">
        <location>
            <begin position="118"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="KOW" evidence="12">
        <location>
            <begin position="210"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="KOW" evidence="12">
        <location>
            <begin position="361"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="KOW" evidence="12">
        <location>
            <begin position="413"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="domain" description="KOW" evidence="12">
        <location>
            <begin position="632"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="domain" description="KOW" evidence="12">
        <location>
            <begin position="904"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="591"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="708"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="9"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="29"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="51"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="716"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="747"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="800"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="958"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7754771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="958" mass="105287" checksum="499D338CC5135A7D" modified="2024-05-29" version="1" fragment="single">YGGGGGRGSSRRRGGKAAKKASGASFFELEADVDTDAEEEEEEDGEDDFIVNERLDPRDEENARRMHRRPLLSPEDEQEDVEALERRIQEKYSRNRTEYDDDDATEVEQQALLPSVRDPKLWMVKCAIGHEREVAVCLMQKCIDRSSELQIRSAIALDHLKNYIYIEADKEAHVKEACKGMRNIYTGSKILLVPIKEMTDVLSVESKAIDISRDAWVRMKIGTYKGDLAQVVDVDNVRQRVTVKLIPRIDLQALANKLEGREVQKKKAFTPPPRFMNVEEAREMHIRVERRRDPMTGDYYENINGMMFKDGFLYKTVSMKSISTQNIQPSFDELEKFRQPSENGENDNSLSTLFANRKKGHFMKGDRVIVIKGDLKNLKGWVEKVEEGTVHIKPNAEGLLTTLAVNEKELCKYFEPGNHVKVVSGAQEGVTGMVVTVKGHVVNIVSDTTKEVLRVFSDNVVESSEVTSGITKIGDFELHDLVQLDNSNFGVIIRVDSEAFQVLKGVADKAEVELVRLRDIKYKIDRKVSAQDRYKNTVSVKDVGRQGPVEHINRGILFIYDRHHLEHAGFICAKAQSCVLVGGSRANGDRNGNPLASRVAQLRTPTRIPHSPGRGAPPLPGGRYRGGRGGKDSLVGTVIKIRVGPFKGYKGRVVDVHGTSVRIELESQMKSIAHTYQIVDTHRYGSGSETPMHPSRTPLHPYQTPMRDAGATPIHDGMRTPMRDRAWNPYAPMSPPRDSWEDGNPGSWGSSPQYQPGSPRSRAYEAPTPGSGWANTPGGSYSEAGTPRDTTPTYGNAPSPYLPSTPGGPAPMTPSSAYLPGTPGGQPMTPGGGGLDMMSPAVGGDHDGPWFLPDILVNVRRPGEDASLGVIREVLLDGACRVALGASGNGEIITANPSEIEVVVPRKSDKIKIMGGAQRGATGKLIGVDGTDGIVKVDESLDVKILDMVILAKLAPLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D5Z9</accession>
    <name>A0AAD5D5Z9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_010155</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily. Type-7 methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005361">
        <property type="protein sequence ID" value="KAI7753637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D5Z9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.270">
        <property type="entry name" value="Methyltransferase, alpha-helical capping domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005299">
        <property type="entry name" value="MeTrfase_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042086">
        <property type="entry name" value="MeTrfase_capping"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31009">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE:CARBOXYL METHYLTRANSFERASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03492">
        <property type="entry name" value="Methyltransf_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7753637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="361" mass="41105" checksum="6C366898D4FF9734" modified="2024-05-29" version="1">MTLIDVLHMSSGSGDSSYAHNSRYTKIVIQETTPFLKRAIKSMANQDVPFDDCFKFADLGCSSGSNTLFLASNIIEIVHEVCQDGKRKIPQFQVCLNDLYVNDFNNVFKLLPDFYKTLKKEKGENFGPYFVSAVPGSFYGRLFPDKSLHLVHSCYSIHWLSQVCVPKGIEDNKLNIYVSRTSPLNVMQAYENQFHTDFTKFLQLRSEEIVPGGRMVLTLRGRSSLDPTSDDYGSFWELLTISLREMLEEGLVQESDINSFNLPFYFPHEDELRSIVQTEESFSLDHMTSFEVDVDQEDTDYTDCGKNTSKAIRAVTETLFASHFGNSIIDVLFKKYEKNAMEHLGAKESKNVTVLVLLTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D6L2</accession>
    <name>A0AAD5D6L2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">No apical meristem-associated C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_026732</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005567">
        <property type="protein sequence ID" value="KAI7752991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D6L2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7752991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="186" mass="21125" checksum="20304669C11C8DDC" modified="2024-05-29" version="1" fragment="single">HKKKKSKTDPAASQEKIKCSANKVGDVSSFGKSKGSTPKDNLSTQKNSGKSCDAATTTSKEAAKSKEDEKTLKINTMTKTVNNSNGKTPPSSSKFSDRGPTKAEKMEYDKERLRLEAEKLKIKADKGQSKIRLQNERLRLKAEKMQLLKKELDIRIMMIDARVMPEMQRLYFEELQREIIMRRSRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D9A2</accession>
    <name>A0AAD5D9A2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Aquaporin NIP2-1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_005507</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the MIP/aquaporin (TC 1.A.8) family. NIP (TC 1.A.8.12) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010004979">
        <property type="protein sequence ID" value="KAI7753980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D9A2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046715">
        <property type="term" value="F:active borate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015267">
        <property type="term" value="F:channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00333">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1080.10:FF:000013">
        <property type="entry name" value="Aquaporin NIP2-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="entry name" value="Glycerol uptake facilitator protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034294">
        <property type="entry name" value="Aquaporin_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000425">
        <property type="entry name" value="MIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022357">
        <property type="entry name" value="MIP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45724">
        <property type="entry name" value="AQUAPORIN NIP2-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45724:SF16">
        <property type="entry name" value="AQUAPORIN NIP2-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00230">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00783">
        <property type="entry name" value="MINTRINSICP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81338">
        <property type="entry name" value="Aquaporin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00221">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="75"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="157"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="190"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="229"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00060753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7753980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="284" mass="29784" checksum="D9193FFDCBF7B509" modified="2024-05-29" version="1">MSEILPNGGGCESSVITTEKPQSRRLKGFILSLVDYPPGFSRKVVAEVVATFLLVFVTCGAAALTTSDDRKVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTLAFATVGHFPWIQVPIYAAAQLTGSISASFALRVLLEQVKHLGTTTPSGTDLQALVMEIIVTFCMMFVTSAVATDSKAVGELAGIAVGSSVCITSILAGPVSGGSMNPARTIGPALASNNFKGIWVYIVGPIFGTISGVLCYSFIRHTNKPIQSVSSFKLRRMKSNDQHVVEKDSLSFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D9R4</accession>
    <name>A0AAD5D9R4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_008252</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the major facilitator superfamily. Phosphate:H(+) symporter (TC 2.A.1.9) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000811">
        <property type="protein sequence ID" value="KAI7755804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D9R4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11654">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="121"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="169"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="216"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="271"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="390"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00044504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7755804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="435" mass="49251" checksum="74C394D9871CFA45" modified="2024-05-29" version="1" fragment="single">QHNLNSGGIYPEKRQRIDVVAEEVVPGSQHKSANRAFQRSILGFLMGCVAGLGGGGVAEIRDLGDTRHQETKKEFPVAISCTNEVTICNDGNTININTKMSNFLDALNMIGKAEKLENYSVLMLTFYLIDVWELGIVYAAGLINIWLGITKLMPFAILFLFDNKYVGSYTMLIISTATSALGTGLLSLSTPSWFYSSITGSCDNYKMKCITEIQKWLFYLALVLIMIGRSSRKATVETLNFKNRAIKQERTHDLFPSAGYWLNNEASALVLAPWSILFGMPSVYTSMALYHLKGKSWSHKIYINEPERSPLTSVLRVVAASILNISQDKPYEDCDETEIDEPLLNRSFSLRFLEKAALQPPHDKGWPNRWTHCSIQEVENAKTIVRMLPVGFMFAFLVLIYTIGMTYFPQQWKRLNLLDYSTVLQIERWTDSRKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DB79</accession>
    <name>A0AAD5DB79_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_007892</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000446">
        <property type="protein sequence ID" value="KAI7756337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DB79"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd17039">
        <property type="entry name" value="Ubl_ubiquitin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47725">
        <property type="entry name" value="OS03G0364000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47725:SF2">
        <property type="entry name" value="UBIQUITIN-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="1">
        <location>
            <begin position="33"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7756337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="136" mass="15641" checksum="982CF58797AB358A" modified="2024-05-29" version="1" fragment="single">KNRNLPKITSSTVSDELQLWSVRSLSELHIIQMAMYIRVKRNKTTYFLQCDATEKILDIKQKLHDLIDQPINDQRLILVSTGEVLDDSKTLADQKVENDAVVALVLRKDDNEFEKVNIVKPDGFCPPRDAETTESW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DC17</accession>
    <name>A0AAD5DC17_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_011835</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000300">
        <property type="protein sequence ID" value="KAI7756694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DC17"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7756694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="222" mass="24789" checksum="1D6C0AD9FA195B3B" modified="2024-05-29" version="1">MQQPDQSQVLGRCLRRAQHRRTRKYTGDLELLLDHINIYYNDSLRYNVASSWIWILVPWTTSDLVPTARSSTLKTLCLDRVVLGITTAKDVNVFSLYLSLSLAIQGGHFKFETSLHFLLSQSKTLILFTPFTSSVRRGTKSSGIMLQQDGRGLRNHVDHSDGKLLNMSKQASLTRSARANFPSKPLPLAQAATGYQFAAQPAKPVTKLALKPLTPVSSYAQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DCZ2</accession>
    <name>A0AAD5DCZ2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">S1 motif domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_034049</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000184">
        <property type="protein sequence ID" value="KAI7757095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47600">
        <property type="entry name" value="NUCLEIC ACID-BINDING, OB-FOLD-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47600:SF2">
        <property type="entry name" value="NUCLEIC ACID-BINDING, RNA-BINDING DOMAIN, S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="174"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="174"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7757095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="613" mass="69473" checksum="62308317195FA3F6" modified="2024-05-29" version="1">MCSITATTSNASNLGFCIPCYINPITFKFLTLTFPLKHQTKLVVFAADNNLGNWDQMELKFHSLIRQNSKITLAKIKDRKSNTEQDKGKRPKEPLVKAPNVILWKSTTFDDSDVEERMSSGLSLKPNLSLQMGKEDDKERFSRMVLVRNLEPLTKNNEVEQAVTIKESTCQTKKVDQAYGDHNRSRNLDSSSEIGKVAATNPDQSQESRDIAADSVSEPSPDATLQGRPERSLGQPVKEMFGIGGRNTKVDSLIPYFATSENLLPGMFVKDDEDVDWKRAQDMIKTTGRGEVELISCCTRGFVVSFGSLIGFLPYRHLATKWKFLAFESWLRGKGLEPATYRKRLGIVGNSDAISVDPEKIKGEISPDMKLEDLLAIYDQEKLEYLSTFVGQKIKVNVILADRESRKLIFSVKPKEQEESIQRKRNLMAKLKVGYTVTCCIKKISYFGIFVEASNRFFAMKFHGMPLRTXXXXIGQVVEAKVHQLDFSLERIYLSLKEITPDPLTESLEAVIGHNAVLDGTLDTDQPDDEWVDLELLVKGLQSYEGIELVTKERFFLSPGFTTPFQVYMVSMFKNQYKLLARSGNKVQEVVVQTWLDTEEMKHAILLCYKRIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DD28</accession>
    <name>A0AAD5DD28_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_014490</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000360">
        <property type="protein sequence ID" value="KAI7756501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DD28"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008353">
        <property type="term" value="F:RNA polymerase II CTD heptapeptide repeat kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07840">
        <property type="entry name" value="STKc_CDK9_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000043">
        <property type="entry name" value="probable serine/threonine-protein kinase At1g54610"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000021">
        <property type="entry name" value="probable serine/threonine-protein kinase At1g54610"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF445">
        <property type="entry name" value="CGMP-DEPENDENT KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="117"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="431"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="565"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="575"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7756501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="585" mass="66605" checksum="F5F5F8E28E9D8D31" modified="2024-05-29" version="1">MGCLNSKNVGRNCNVSIYDRSMIIHGASESLKGQKVRKYIRRNGFKPLDEIKEGLDDEKTSDHIRKAFVKSSNNNKHWRYVGRSSEGEGGADAWPAWLRAAAGDAINGWLPLKSDNFERLEKIGQGTYSNVYRARDLQTGRIVALKKVQFDNFKPESVSFMAREIKILRRLDHPNVMKLEGIITSKLSCDIYLVFEYMEHDLCGLLSCPHVKFTASQIKCYMLQLLKGIQHCHSRGVLHRDIKSSNILINNKGVLKIADFGLANFFDDRNKQPLTNRVVTLWYRPPELLLGSTSYGPCVDMWSVGCVFAELFNGRPILKGRTELEQLHKIFKLCGSPTDEYWKNSGLPLAALFRSQSCYENNIRERCKELPTTVVDLIYTLLSVEPEKRPTARSALHDAYFYAKPYACDPASLPTYPPNKEINAKFREAAQRKTDGGKSRPSGCSRKPRKALSCKVIPEDRNLYMFRSRGSLNSKAVSEVSQRGDSIGTVPAQAVASYGRFMWETRRQEHLQRYQSLMQPDFLNSTQVYKPRDDQEPEISRHMIMLHQLRSADVMQLEDYIRAGAHQSHSSRGAVAHDKRVRSEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DEI2</accession>
    <name>A0AAD5DEI2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_007408</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000033">
        <property type="protein sequence ID" value="KAI7758044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DEI2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010011">
        <property type="term" value="F:auxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009734">
        <property type="term" value="P:auxin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010152">
        <property type="term" value="P:pollen maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22159">
        <property type="entry name" value="F-box_AtTIR1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.50:FF:000006">
        <property type="entry name" value="Transport inhibitor response 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000029">
        <property type="entry name" value="Transport inhibitor response 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041567">
        <property type="entry name" value="COI1_F-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041101">
        <property type="entry name" value="Transp_inhibit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16134:SF151">
        <property type="entry name" value="F-BOX DOMAIN, LEUCINE-RICH REPEAT DOMAIN SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16134">
        <property type="entry name" value="F-BOX/TPR REPEAT PROTEIN POF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18511">
        <property type="entry name" value="F-box_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18791">
        <property type="entry name" value="Transp_inhibit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0927">Auxin signaling pathway</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="F-box" evidence="8">
        <location>
            <begin position="108"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="38"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7758044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="671" mass="75221" checksum="434C7F047579F8ED" modified="2024-05-29" version="1">MNPHRKRAKTVDRVDSADSALVSSESTQMRPFPSKPLSVDRVKSVESSESGRMRPFKDKILFVDRVDSVESSESSRTSQFSDKISGFDRADSVEARVDSAGSTRMSRFPDEVLERVLALVDSHKDRSAVSLVCKEWYNAERWSRRHVFIGNCYSVSPEIVAARFPRIRSVTLKGKPRFSDFNLVPEDWGADVQPWLSVFSTAYPFLEELRLKRMAVTDESLEFLAKKFHGFKALSLLSCDGFSTDGLKAIATHCKNLTELDIQENGIDDLGGHWLSCFPDSLTSLEILNFASLNSEVSFDALEKLVSRCKSLRVLKVNRNISLDQLQKLLSHCPQLMELGTGTFMQELISGPVTEFKNMFSNCRNLITLSGLWDATPLYLPVIYPACARLTFLNLSYATLRSDELSNLLSHCKSLRRLWLLDTVGDKGLEAVGSCCPLLEELRVFPADPFDQEILLGVTESGFVSVSRGCPKLRYVLYFCRQMTNAAVATIAQNCPDFTHFRLCIMNPGQPDYLTKEPMDEAFGAIVKTCTKLQRLAISGLLTDRTFEYIGKYAKNLETLSVAFAGSSDLGMEYVLQGCQKLRKLEIRDCPFGNAALLSGLTKYESMRSLWMSACNVTMNACRVLAKERPRLNVEVMRDEEGGDDSQANKVYVYRTVAGPRKDAPPFVLTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-06-18" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G274</accession>
    <name>A0AAD5G274_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_031009</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HD-ZIP homeobox family. Class III subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011744">
        <property type="protein sequence ID" value="KAI7726129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14686">
        <property type="entry name" value="bZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000197">
        <property type="entry name" value="Homeobox-leucine zipper protein REVOLUTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044830">
        <property type="entry name" value="HD-Zip_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45950">
        <property type="entry name" value="HOMEOBOX-LEUCINE ZIPPER PROTEIN ATHB-14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45950:SF15">
        <property type="entry name" value="START DOMAIN, HOMEODOMAIN-LIKE, MEKHLA, START-LIKE DOMAIN PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 9 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 9 10" id="KW-0371">Homeobox</keyword>
    <keyword evidence="8 9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="12">
        <location>
            <begin position="13"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="9">
        <location>
            <begin position="15"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="129"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="129"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7726129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="181" mass="21051" checksum="82907A0BFAE06713" modified="2024-05-29" version="1" fragment="single">MMAMACKDGKGAIMDNGKYVRYTPEQVEALERLYHECPKPSSLRRQQLIRECPILSNIEPKQIKVWFQNRRCREKQRKEASRLQTVNRKLSAMNKLLMEENDRLQKQVSHLVYENGYFRQHTQNTALSTKDASCESAVTNGQHRLTPQHPPRDASPAGLVEVHFMLXXXHEHVVWLLSSLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G2E5</accession>
    <name>A0AAD5G2E5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GDSL esterase/lipase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_025295</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011785">
        <property type="protein sequence ID" value="KAI7726025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G2E5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01837">
        <property type="entry name" value="SGNH_plant_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051058">
        <property type="entry name" value="GDSL_Est/Lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035669">
        <property type="entry name" value="SGNH_plant_lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45648:SF167">
        <property type="entry name" value="GDSL ESTERASE_LIPASE LTL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45648">
        <property type="entry name" value="GDSL LIPASE/ACYLHYDROLASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_4G14700)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7726025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="366" mass="40501" checksum="1343D6194CAB802C" modified="2024-05-29" version="1" fragment="single">NVALKADARAFFVFGDSLVDNGNNNYLTTTARADNVPYGVDYPTRRPTGRFSNGYNIPDLISQAIGSEATLPYLSPMLTGQKLLVGANFASAGVGILNDTGVQFFNFYSKAGYNNLFMVSSNSQVNIIKMPQQLAYLEQYKQRVSEIIGQQETERLVSQSLTLMTLGGNDFVNNYYLVPFSARSRQFALADYVPYIVSEYRKILMRLYELGLRRVLVTGTGPLGCVPAELAQHSRNGECSEELNRASGLFNPQLQAMLDSLNQEIGSHTFIGVNIRQSSIDFISDPERYGFVTAKVACCGQGPYNGIGLCTPRSNLCPNRDIYAFWDPFHPSERANRIIVQQIMTGSTDYISPMNLSTIMALDAVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G4Q5</accession>
    <name>A0AAD5G4Q5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">BAH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_009514</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011386">
        <property type="protein sequence ID" value="KAI7727408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G4Q5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.490:FF:000017">
        <property type="entry name" value="Bromo-adjacent homology (BAH) domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001025">
        <property type="entry name" value="BAH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043151">
        <property type="entry name" value="BAH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47073">
        <property type="entry name" value="PROTEIN ANTI-SILENCING 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47073:SF2">
        <property type="entry name" value="PROTEIN ANTI-SILENCING 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01426">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00439">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51038">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BAH" evidence="1">
        <location>
            <begin position="43"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7727408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="474" mass="54067" checksum="5D52A3ED07CFF656" modified="2024-05-29" version="1">MSTLEEENATVSGDIEFRWGRKGRVGKKNPNIQYYESFTYDGVDYFLYDSVYLWSGDQYLPHIGKVIKIYETPRLKKMVKVVWYLRPTEVQKWLKGDHHLNNELLLASGEGNGLSNFNPLETICGKCNVVCQSKDERNPKVSMEELKLSDFVFYQTFDVETCRLSEKFPDKIAGVEVGHFFNPKTKGNHGVIRKSNSSSMCDTDNSSTIVSGVPASSSCENHHSMKSKFQEFEVGNKKSIEADSQSIMLKKRPKLLQEFEVDTKKSIEADSQSIMLKKRPKLDTSVWFKKLPWEDRIQKAHEVGSLVLLENLYHSFTSSEVEEIVLDAFNIKVNAKMIQWSAYSSPHNGQALVIFNSKDEADYAIFELKTRCLVIGAARVVVGSRPSLKEPGNHNTSKYFGHITIDAIKRKLTVEMKNAVSTSHHSQPNTIAFEMAMEWWAQQKMWELCRDALYKKQAEEIAKLKGRLKGSGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G4T8</accession>
    <name>A0AAD5G4T8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cupin type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_028698</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011152">
        <property type="protein sequence ID" value="KAI7728769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G4T8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02245">
        <property type="entry name" value="cupin_7S_vicilin-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006045">
        <property type="entry name" value="Cupin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050253">
        <property type="entry name" value="Seed_Storage-Functional"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31189">
        <property type="entry name" value="OS03G0336100 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31189:SF50">
        <property type="entry name" value="RMLC-LIKE JELLY ROLL FOLD PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00190">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00835">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cupin type-1" evidence="1">
        <location>
            <begin position="57"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7728769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="234" mass="25940" checksum="BB78C70CD6CEB17E" modified="2024-05-29" version="1" fragment="single">VSEEVIEELRGGENQPLIVDGQPEASASMGYVGFRGIKALVESNKKTKKMTKKMKAYNVFKEGHDVETCFGWSVIVTSKELDVLKDTNFGVFVVNLAKGSMMAPHWSPTTVEIAIVLHGQGVIEVVCPGIASETGCKNSRLRVEEGDVFVVPREHPMAQMSFNNDSFVFMGLMLNEKKESPQYLAGKLSILQRLDRKVLEKSFNVGNTTLDWILSNEKEDIIYECVSCAEEAQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G7K9</accession>
    <name>A0AAD5G7K9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Amino acid transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_013743</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010318">
        <property type="protein sequence ID" value="KAI7732055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G7K9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF705">
        <property type="entry name" value="AMINO ACID TRANSPORTER AVT1I-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="70"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="118"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="164"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="187"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="226"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="259"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="302"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="342"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="368"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="396"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="7">
        <location>
            <begin position="40"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7732055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="429" mass="46841" checksum="6B4800212CFB0204" modified="2024-05-29" version="1">MASEKLEDYSVTIPLLINHETGTKAEEQHVSTATTVTKGTSSFFNTCFNGLNALSGVGILSVPYALASGGWLSLLLLFAIAISTFYTGILIKRCMDTDPMIRSYPDIGDRAFGKTGRIIVSVTMNIELYLVATGFLILEGDNLSNLLPEIDFDIYGIHVGSKKGFVVIVSAIIIPTIWLNNLSILSYISASGVLASLVILFSIMWVGEFDGVGFKEKGTMVNWNGMPSAVSLYAFCYCAHPVFPTLYTSMKNQHQFSKVLLVCFVFCTTTYSLMAVVGYLMFGSEVESQITLNLPTNKISSIVAICTTLVNPIAKYALMMTPIVHTIEERFLSFYNTRMFSLLIRTALVISTVIVALALPFFGYLMSLVGAFLSVTASVILPSLCYLKISGTYRRFGLELVLIGFIVFVGMVVAIVGTYTSLADIARNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G8A6</accession>
    <name>A0AAD5G8A6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_027533</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O-methyltransferase family. COMT subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010582">
        <property type="protein sequence ID" value="KAI7730963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G8A6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016461">
        <property type="entry name" value="COMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001077">
        <property type="entry name" value="COMT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012967">
        <property type="entry name" value="COMT_dimerisation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11746">
        <property type="entry name" value="O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08100">
        <property type="entry name" value="Dimerisation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00891">
        <property type="entry name" value="Methyltransf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005739">
        <property type="entry name" value="O-mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51683">
        <property type="entry name" value="SAM_OMT_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="O-methyltransferase dimerisation" evidence="7">
        <location>
            <begin position="21"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="O-methyltransferase C-terminal" evidence="6">
        <location>
            <begin position="134"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00034481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR005739-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7730963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="356" mass="39419" checksum="059A9C40B011A476" modified="2024-05-29" version="1">MNTQNGTDGDAMLRGQAQIFQYIYGALDGMAIRCCVELRIADIINNHGHPTTLSEISTAIGSPSINIDGLRRLMTFLVNRKVFNETVQSEEGESEPLYSLNDCSRWLLRDTNVTLAPLVMLRTDPVSNLPLHVLSRSIKEGGTAFKAAHGIEMFEFCSLNSEFNRVFNEAMACNAKFTTNAVISHYKDGFLGSKGSMVDVGGGTGTAISEIVKAYPHLKGINFDLSHVIATAPTYDGITHVAGDMPKSIPSTETIFLKCILHNWSDGDCVKILQNCRKALSKENGKLIIVEIVQQPTTRDIFDDTRHTFDIVMFAHFSGGRERSEREWKKLLDEGGFSRYNVIKIPALHSIIEAFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GA07</accession>
    <name>A0AAD5GA07_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Serpin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_024395</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the serpin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009826">
        <property type="protein sequence ID" value="KAI7734112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GA07"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.497.10">
        <property type="entry name" value="Antithrombin, subunit I, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023796">
        <property type="entry name" value="Serpin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000215">
        <property type="entry name" value="Serpin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036186">
        <property type="entry name" value="Serpin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042178">
        <property type="entry name" value="Serpin_sf_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461:SF211">
        <property type="entry name" value="GH10112P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461">
        <property type="entry name" value="SERINE PROTEASE INHIBITOR, SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00079">
        <property type="entry name" value="Serpin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56574">
        <property type="entry name" value="Serpins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="domain" description="Serpin" evidence="3">
        <location>
            <begin position="61"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7734112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="185" mass="20271" checksum="3129316F5CBEA709" modified="2024-05-29" version="1">MAASWIPKRQKTLYTLLFLSNIILLVVFNSSLLGLNSKDDSIIFPSQDDLVVIRSLEQSSNQTDVSITLAAHLLSKKHDYNIIFSPLSIRVVLNLIAAGSSGQTLDQLLAFLKANTTDDLNSYSQLVSKILADGGPNGGPRLSFANGVWLDNMLSLKPSFKEVLDTVYKASSKQVNFQNKVSVKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GAH9</accession>
    <name>A0AAD5GAH9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein FAR1-RELATED SEQUENCE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_033483</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Putative transcription activator involved in regulating light control of development.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the FHY3/FAR1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010092">
        <property type="protein sequence ID" value="KAI7733078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GAH9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004330">
        <property type="entry name" value="FAR1_DNA_bnd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031052">
        <property type="entry name" value="FHY3/FAR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018289">
        <property type="entry name" value="MULE_transposase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006564">
        <property type="entry name" value="Znf_PMZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31669">
        <property type="entry name" value="PROTEIN FAR1-RELATED SEQUENCE 10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31669:SF42">
        <property type="entry name" value="PROTEIN FAR1-RELATED SEQUENCE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03101">
        <property type="entry name" value="FAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10551">
        <property type="entry name" value="MULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04434">
        <property type="entry name" value="SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00575">
        <property type="entry name" value="ZnF_PMZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50966">
        <property type="entry name" value="ZF_SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SWIM-type" evidence="8">
        <location>
            <begin position="506"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="623"/>
            <end position="659"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7733078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="734" mass="84089" checksum="E6694FBB9326FD4E" modified="2024-05-29" version="1">MDGSTVSGSSNAEHQDAMEFDSNEAAYEYYKEYAKSVGFGTAKLSSRRSRASKEFIDAKFSCIRYGNKQQSDDAINPRPSPKIGCKASMHVKRKPNGKWYIHSFVKEHNHELLPSQSHFFRSHRNSDPLKNDAKVRRKSILSTMSKQYGMYQSSGLENYIRNQSDRGRCLALEEGDAQILLELFVTMQEENPDFFYSVDLNEEHQLRNVFWVDAKGIEDYKNFNDVVSFDTMYFVSKYKIPLVLFIGVNQHCQPTLLGCALIADETLFTFVWLFQTWFIAMGSRAPNVILTDQSNSIEAAIAAVFPGTRHFYNLWNVLEKVSRQLDYLNPWHDTFTVKFNKCIHRSWTEEEFDRRWAKLVNKFNLNEDAWFQSLYHDRKLWAPTFIRNISLAGLYASSRSESPNSFIDKYIHSETSLREFLEQYKVIMEDLYEEQAKSNFDAWHDTPELKSPSPFEKQLSFVYTHEIFKKFQVEILGASACHLKVDKQDGDSITCIVKEYETSQDYMVEWNGAQLDICCSCRSFEFKGYLCRHAIVVLQMSGVFNIPVKYVLQRWTNAATSRYPISENLENVQTKTRRLNDLCRRAIILGEEGSISQESYKIALGAIKDALAQCSNVNNSAANEGQGVPAVSKEPGSNLNVNKGIKRTEPGTEKSNSNDTAVMNEKVPAQPEVVNIGIDAGFHQMELPNTRHLQLHNMMPPQLQGVVPTMFHNVASTQFHNVTSAHLQNSRLPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GBA9</accession>
    <name>A0AAD5GBA9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_015404</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the iron/ascorbate-dependent oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009439">
        <property type="protein sequence ID" value="KAI7735747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GBA9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.330:FF:000018">
        <property type="entry name" value="2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050295">
        <property type="entry name" value="Plant_2OG-oxidoreductases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47991">
        <property type="entry name" value="OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0408">Iron</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="5">
        <location>
            <begin position="192"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7735747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="341" mass="39129" checksum="F0E7E96E1B25AB1F" modified="2024-05-29" version="1">MNHQVQDIAAHCEQLPERFIRKEDEEYGNVTNSNASSPAKIPVIDVSLLTSSPLELNRLKIAITTWGCFQAINHGIEGSFLDKVREVSRLFFDLPAEEKKKYLREENDLEGYGNDMVLSDHQILDWTDRLYLTVFPKDQRMLQFWPKSSTEFREVVDDYCSKIELINQVILKAMARSLNMEDNCFLDQYGTTNSKMFARFNYYPPCPWPDKVLGVKPHADGSAVTFLLQDKEVEGLQVLKDGQWFGVPIVPDALTVNVGDQIEIMSNGIFKSPVHRVLVNTKNQRITLAVFCMPQTEKDIGPVDGLITDDTPRLYKNVTYSRDFFFQNYQHGKRTIDACKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GBU7</accession>
    <name>A0AAD5GBU7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Hflx-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_030950</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009436">
        <property type="protein sequence ID" value="KAI7735759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GBU7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01878">
        <property type="entry name" value="HflX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11060:FF:000003">
        <property type="entry name" value="GTP-binding protein chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001888">
        <property type="entry name" value="GTP-binding protein chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11060">
        <property type="entry name" value="GTPase HflX, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030394">
        <property type="entry name" value="G_HFLX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032305">
        <property type="entry name" value="GTP-bd_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016496">
        <property type="entry name" value="GTPase_HflX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025121">
        <property type="entry name" value="GTPase_HflX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042108">
        <property type="entry name" value="GTPase_HflX_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03156">
        <property type="entry name" value="GTP_HflX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10229">
        <property type="entry name" value="GTP-BINDING PROTEIN HFLX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10229:SF8">
        <property type="entry name" value="GTPASE HFLX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16360">
        <property type="entry name" value="GTP-bdg_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13167">
        <property type="entry name" value="GTP-bdg_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51705">
        <property type="entry name" value="G_HFLX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hflx-type G" evidence="6">
        <location>
            <begin position="324"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="66"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="577"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7735759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="577" mass="64135" checksum="6B307A699CAD50FD" modified="2024-05-29" version="1" fragment="single">NAAGNILVSPRIDHPKPSDLLTLCSSNSKTLSIQSNKQKSLTKMFSIIINRTRPSTRFIPQTLTLQSTHTRHKHNEPDPDEPNTDPDAAPKLFVVQPRFRPDSLLKIKLDEALNLANSLEEQRDGYYSTQFSDKDSPPHVVVQNPAYRITRADTYFGPGTVDNIKCHLNALDSKGGVDAVFVNGILTGIQQRNLERAWGKAVLDRVGLIIEIFHAHAQTKEAKLQAELAALMYKRTRLVRIRGSGGRLTFGAAGEAEVVSAKGRGSGGRGFISGAGETELQLQRRRIIERKNQLMSEIQEVRRTRALQRASRKRYGGTHGQHMPTVAIVGYTNAGKSTLVAALSDTHLFCDNRMFATVDTKVRHVILPSGKKVLLSDTVGFISDLPVQLVEAFHATLEEVVEADLLVHVLDSSAPNRDEQRESVLQVLEQIGVSNEKLENMIEVWNKIDLEEAAMTVNEESRCEMNEDLDGWLSTGDEVNWDDASASFVGWKAFEDQPKDVSILSNEVRSDLKPRHEVNSSPSPTSHVKTSGITRVGLQELLELIDDKLKTDKVVERSVFDRKWRPPQKQDTGVVVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GE83</accession>
    <name>A0AAD5GE83_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">M8C21_032349</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">AA19_3_7</strain>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010009168">
        <property type="protein sequence ID" value="KAI7736918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GE83"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001023">
        <property type="entry name" value="Os07g0541700 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000207">
        <property type="entry name" value="proline-rich receptor-like protein kinase PERK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047117">
        <property type="entry name" value="PERK1-13-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47982:SF68">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47982">
        <property type="entry name" value="PROLINE-RICH RECEPTOR-LIKE PROTEIN KINASE PERK4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 13 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="46"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="189"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="85"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="432"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="15">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="87"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="449"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="18">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAI7736918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="465" mass="50654" checksum="020D62C364E43104" modified="2024-05-29" version="1" fragment="single">APPPPPPPPPPPPPPPPPPPPPPPLPPGHHKLSPPRNHSGEANNDVLLTVGAAAAAGLMFFIMILCVMAVLKKRRRKRPEEMYYYNDNDHTNDHHIQENHSNQPQPPNDVHLKMPPVPPPVSGPKSAEYSWVASPPPPPPGMLSDEFNNSSAFSGPYQAPLPPPHPTVALGFNQNTFTYDELAAATQGFDNSLLLGQGGFGYVHKGVLPNGKEIAVKSLKSTSGQGEREFQSEVEIISRVHHRHLVSLVGYCIAGQQRLLVYEYIPNNSLDYHLYGKDAPVMNGSTRLNIALGAAKGFAYLHEDCQPRIIHRDIKAANILIDEHYEAKVADFGLAKLSTDTDTHVSTRVMGTFGYARPILTQAVEGGSYVELVDKRLANNYKHEEMNRMVVCASACLAHSAKRRPKMSQVARALEGDATLDDLNWGWTGGMSSAGSSEAELHKKYGKPTTRSQEYSSGDSSGPFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GGX7</accession>
    <name>A0AAD5GGX7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SHSP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_030083</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008083">
        <property type="protein sequence ID" value="KAI7742077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GGX7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06472">
        <property type="entry name" value="ACD_ScHsp26_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031107">
        <property type="entry name" value="Small_HSP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11527">
        <property type="entry name" value="HEAT-SHOCK PROTEIN 20 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="SHSP" evidence="4">
        <location>
            <begin position="62"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7742077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="191" mass="21855" checksum="9E854C1A273A6211" modified="2024-05-29" version="1">MAMSKTQTTTFLMLTTMAFMMINIPTKTNALIPYNRPLWDMMLPNEDPFKILEQNPLSIPKNLDTINLARADWKETSNHHVITLDVPGMKREEIKIEVEENRVLKVSGERKVDEEAEGDRWHRVERTSGKFWRQFRLPANADVDKIAAHLEDGVLKIKVPKLAQVKKQSKVIDIMGQESSGEDIKATKTTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GHL8</accession>
    <name>A0AAD5GHL8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glutathione S-transferase</fullName>
            <ecNumber evidence="3">2.5.1.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_025648</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Is involved in the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 3">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the GST superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010008157">
        <property type="protein sequence ID" value="KAI7741609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GHL8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03058">
        <property type="entry name" value="GST_N_Tau"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000014">
        <property type="entry name" value="Tau class glutathione S-transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045073">
        <property type="entry name" value="Omega/Tau-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11260">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE, GST, SUPERFAMILY, GST DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11260:SF793">
        <property type="entry name" value="GLUTATHIONE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="4">
        <location>
            <begin position="7"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00047960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU369102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7741609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="126" mass="14477" checksum="01C9E7299D0C72DD" modified="2024-05-29" version="1">MSSTEESEVVVLGWWVSMYTTRVQIALAEKGIDYEYVEHDLFGDKSPLLLKHNPIYKKVPVFIHKGKPICESLIIVQYINETWKDGCSLLPSDPYLKSQAAFWADYIDKHFCIRVCPYAGLVKYGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GIA7</accession>
    <name>A0AAD5GIA7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Serine hydroxymethyltransferase</fullName>
            <ecNumber evidence="8">2.1.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_021114</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Interconversion of serine and glycine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + glycine + H2O = (6S)-5,6,7,8-tetrahydrofolate + L-serine</text>
            <dbReference type="Rhea" id="RHEA:15481"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="EC" id="2.1.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 8">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 8">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 8">Belongs to the SHMT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.1" evidence="8"/>
    <dbReference type="EMBL" id="JAMZMK010007687">
        <property type="protein sequence ID" value="KAI7743770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GIA7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004372">
        <property type="term" value="F:glycine hydroxymethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019264">
        <property type="term" value="P:glycine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00378">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000002">
        <property type="entry name" value="Serine hydroxymethyltransferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001085">
        <property type="entry name" value="Ser_HO-MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049943">
        <property type="entry name" value="Ser_HO-MeTrfase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019798">
        <property type="entry name" value="Ser_HO-MeTrfase_PLP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039429">
        <property type="entry name" value="SHMT-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680:SF45">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00464">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00096">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="7 8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Serine hydroxymethyltransferase-like" evidence="10">
        <location>
            <begin position="67"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="Serine hydroxymethyltransferase-like" evidence="10">
        <location>
            <begin position="397"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="domain" description="Serine hydroxymethyltransferase-like" evidence="10">
        <location>
            <begin position="611"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="domain" description="Serine hydroxymethyltransferase-like" evidence="10">
        <location>
            <begin position="941"/>
            <end position="994"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="28"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="574"/>
            <end position="594"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7743770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="1047" mass="116853" checksum="58740A6487C712CC" modified="2024-05-29" version="1">MDLNKESKIGDEEDEEFRILGHSMCLKRRRDKDRDSIDSASSHTQSKRCQTLESRRQVVRAWGNQSLQEADPDIFELLDKEKRRQYKGIELIASENFVCKAVLEALGSHVANKYSEGMPGARYYAGNQYIDEIEMLCRERALEAFGLDSESWGVNVQPYSCTSANFAVYTGLLLPGDRIMGLDTPSGGNTSHGYYTPNGMKVSGASIFFESLSYKVNPKTGIIDFEKLEERAVDYRPKILICGGSSYPREWDYSKFRQITDKCGAVLMCDMAQISGLIAAKECASPFEFCDIVTSTTHKSLRGPRGGIIFYRKGPKSRKSGMLPSQGDGNDKYDFEEKINFAVCSALQGGPHNNHIAALAIALKQLATPEYQEYMQQLGINGVVWNKLKPSHLPLRKNLEKVCEMCHITVNKTAIFDDNGTLVPGGVRIGTPAMTSRGCLESDFETMADFLCRAAQITSSVQREHGKMVKSFLKGVENNKDIIELRTQVENFATQFSMPGQDLYLPPLGHKNNLELIDMSPDPTRHQQPNTALGRSRVQDTVETMDLNKESKIGDEEDEEFRILGHSMCLKRRRDKDRDSIDSASSHTQSKRCQTLESRRQVVRAWGNQSLQEADPDIFELLDKEKKRQYKGIELIASENFVCKAVLEALGSHVANKYSEGMPGARYYAGNQYIDEVEMLCRERALEAFGLDSESWGVNVQPYSCTSANFAVYTGLLLPGDRIMGLDTPSGGNTSHGYYTPNGMKVSGASIFFESLSYKVNPKTGIIDFEKLEERAVDYRPKILICGGSSYPREWDYLKFRQIADKCGAVLMCDMAQISGLIAAKECASPFEFCDIVTSTTHKSLRGPRGGIIFYRKGPKSRKSGMLPSQGDGNDKYDFEEKINFAVCSALQGGPHNNHIAALAIALKQLATPEYQEYMQQLGINGVVWNKLKPSHLALRKNLEKVCEMCHITVNKTAIFDDNGTLVPGGVRIGTPAMTSRGCLESDFETMADFLCRAAQITSSVQREHGKMVKSFLKGVEHNKDIIELRTQVENFATQFSMPGQDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GIK1</accession>
    <name>A0AAD5GIK1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_006680</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the fatty acid desaturase type 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007599">
        <property type="protein sequence ID" value="KAI7744055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GIK1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016717">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03507">
        <property type="entry name" value="Delta12-FADS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005804">
        <property type="entry name" value="FA_desaturase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021863">
        <property type="entry name" value="FAS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012171">
        <property type="entry name" value="Fatty_acid_desaturase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32100">
        <property type="entry name" value="OMEGA-6 FATTY ACID DESATURASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11960">
        <property type="entry name" value="DUF3474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00487">
        <property type="entry name" value="FA_desaturase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="177"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="221"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="250"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acid desaturase N-terminal" evidence="8">
        <location>
            <begin position="16"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acid desaturase" evidence="7">
        <location>
            <begin position="83"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF11960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7744055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="382" mass="44048" checksum="9F2CBFD010607574" modified="2024-05-29" version="1">MGSGGRATDTNNSGNEVVIKRAPSTKPPFTLGDIKKAIPQHCFNRSLIKSSSYLFVDLFFSCLFYYVAATYIIPLSSPYSYVAWPVYWILQGSVQMGLWVIGHECGHQAFSDYPWLNDTIGYFLHTSMLAPYFSWKYSHRRHHSNTGSLENDESFVPKKKSSLNSFSKLLNTPLGRLFRLVILCTIGWLLYVCFNVSGRKYEKFANHFDPKSPIYNDRERVQILVTDIGLVVTSYGLYKLALAQGFTWLVLVYFAPLVIVYGFLVVITWLHHTHRSLPHYDSTEWNWLRGALATIDRDYGVFNTVLHHITDTHVAHHLLFTIPHYHAMEATKAIKPILGEYYQFDDTPIIKAMWREATECFFVEADEAEDKSKGVYWFNNKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GJ39</accession>
    <name>A0AAD5GJ39_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_010515</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007462">
        <property type="protein sequence ID" value="KAI7744700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GJ39"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7744700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="76" mass="8143" checksum="5D21A12DDE572F41" modified="2024-05-29" version="1">MFHQDDTNVAPNNNDPNKQVEIINIPSDESDADDDVPVNSPAVEQHDAAVDPNEINNAADVSMDSTGVEDPITCPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GK21</accession>
    <name>A0AAD5GK21_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_020481</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007134">
        <property type="protein sequence ID" value="KAI7745795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GK21"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="80"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7745795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="101" mass="11170" checksum="E985EFE779E5FE9F" modified="2024-05-29" version="1" fragment="single">GRSYWNSNGVHEKSEVVDEVVWAYESSIANIMDEFVPNESLSMVFVDAACLFLRYPDSSGSHQHQGLTLDQPAAYGLGDESSKRVREDENGKRANGVGTEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GNF6</accession>
    <name>A0AAD5GNF6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GPI-anchored protein LLG1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_002335</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006763">
        <property type="protein sequence ID" value="KAI7747414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GNF6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058888">
        <property type="entry name" value="LLG1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039307">
        <property type="entry name" value="LORELEI-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31533:SF2">
        <property type="entry name" value="GPI-ANCHORED PROTEIN LLG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31533">
        <property type="entry name" value="GPI-ANCHORED PROTEIN LLG1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26578">
        <property type="entry name" value="LLG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042209473" description="GPI-anchored protein LLG1-like domain-containing protein" evidence="2">
        <location>
            <begin position="21"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="149"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="GPI-anchored protein LLG1-like" evidence="3">
        <location>
            <begin position="49"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF26578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7747414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="169" mass="18439" checksum="889123206B144D4A" modified="2024-05-29" version="1">MKILLISFLLLLCCISTCFSSPVSISDGVFTSEALIGRNLLQAKKPCPVNFEFMNYTIITSRCKGPQYPAKLCCDAFKDFACPYSDDLNDLSNDCSSTMFSYINLYGNYPPGLFSNLCRDDKIGLICPASAPGTGKNSVGADSNSSPNIHTWSILTLTVGFISFVFLFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GRB0</accession>
    <name>A0AAD5GRB0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glucosidase II beta subunit N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_023166</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005717">
        <property type="protein sequence ID" value="KAI7752245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GRB0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017177">
        <property type="term" value="C:glucosidase II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006491">
        <property type="term" value="P:N-glycan processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039794">
        <property type="entry name" value="Gtb1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028146">
        <property type="entry name" value="PRKCSH_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12630:SF1">
        <property type="entry name" value="GLUCOSIDASE 2 SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12630">
        <property type="entry name" value="N-LINKED OLIGOSACCHARIDE PROCESSING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12999">
        <property type="entry name" value="PRKCSH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041919399" description="Glucosidase II beta subunit N-terminal domain-containing protein" evidence="3">
        <location>
            <begin position="28"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="domain" description="Glucosidase II beta subunit N-terminal" evidence="4">
        <location>
            <begin position="19"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="193"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="193"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="243"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="272"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="307"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="345"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="363"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="389"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7752245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="423" mass="47671" checksum="FDA6C8C8CB803879" modified="2024-05-29" version="1" fragment="single">MKKTYTQSFILLVLCSILVHRSTVVLSENILGVAPKDEEYYKGLSIKCRDGSRSFTKAQINDDFCDCADGTDEPGTSACPSGKFYCRNAGHSPILIYSSRVNDGICDCCDGSDEYDGKIKCKNTCWEAGKVARDRLRKKIATFREGLTIRKHEVEQAKLSAAKDEEELSRLKNEENILKGLVQQLKERKEQIEKAEEKERIQRENEEKKKKEAEEAKLKEEKHEEKVNVEEQEAVGSNSDDEAGAHDHSPSDQDLNKAPADIEAAVAHNDDGDASIDNMEEEHAEDNEEPSEVAHEHEPEELSSAAHEYDHSHAPGSKEEDASEDTNSLSREELGRVIGSRWTGKKAEEHEEDAGTARHHDDDNDDDEYDDTSDNAHDEEYGYDTETEEDHHKYEDDDHMNDVGDDDSSGSHKYESDDEIDMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GS99</accession>
    <name>A0AAD5GS99_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">40S ribosomal protein S26</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_007457</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS26 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005436">
        <property type="protein sequence ID" value="KAI7753462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GS99"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1740.20:FF:000002">
        <property type="entry name" value="40S ribosomal protein S26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1740.20">
        <property type="entry name" value="Ribosomal protein S26e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000892">
        <property type="entry name" value="Ribosomal_eS26"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047864">
        <property type="entry name" value="Ribosomal_eS26_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038551">
        <property type="entry name" value="Ribosomal_eS26_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12538">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12538:SF0">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01283">
        <property type="entry name" value="Ribosomal_S26e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00733">
        <property type="entry name" value="RIBOSOMAL_S26E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="86"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="88"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7753462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="125" mass="14136" checksum="AC616E0BE29F44F5" modified="2024-05-29" version="1" fragment="single">MTFKRRNGGRNKHGRGHVKFIRCSNCGKCCPKDKAIKRFNVRNIVEQAAVRDVQEACAFDQYTLPKLYLKVQYCVSCAIHSKVVRVRSRTDRRNREPPKRFSRPRDDAPKTGQAPRAGGAGGPPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GSB8</accession>
    <name>A0AAD5GSB8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_014293</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005406">
        <property type="protein sequence ID" value="KAI7753522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GSB8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47447">
        <property type="entry name" value="OS03G0856100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47447:SF27">
        <property type="entry name" value="PENTACOTRIPEPTIDE-REPEAT REGION OF PRORP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13812">
        <property type="entry name" value="PPR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="128"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="163"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="198"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="233"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="408"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7753522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="559" mass="63898" checksum="CBE1359EE7BB4DDD" modified="2024-05-29" version="1">MVSSGSLFRFLKTHKPLLCFCINSCFHSQTQFKQTNFIKKITANSKKTIKSTTKESLTTPRLYMKDTITTISNILRYSTWDSAQQQLQSLPIKWDSYTINQVLKTHPPMHKSWLFFNWTSGLRTFKHDHFTYTTMIDIFGEARRISSMWFVFRQMQEKGVKVDVVTYTCMLHWLSNDGDVDGAVKLWEEMKEKGVGLTVVSYTAYMKVLFDCGRVKEAVGVYKEMIRAGCVPNCFTYTVLMEHLAACGKFNEVLDIFIKMQDAGVQPDKATCNILVESCCKAGESDVLLKESNRHLSIHYEKPQPLVNNGVSDTVSIDQLLVLYLLNRKHFVGVDSLFKDMMDRNVILTPEIVSSVIDTYSAHERPHGAVLAYEYVNQSALLIYKLGCANKPVSASKVFDFLPDNEKNTTSYTALMAAYFASRNTSKGLLIFKEMKDVGIPIVVGTYNVLLAGLEKSGWVVEFEYYRKEKKQMQNISFSENQESAEEMTCNLLFGLGLERCSPLSVFVSLQIGRKQENKENFIIEACFYQMQEKWVRIDVVTYTSMLHWLSNDGVVLSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GT80</accession>
    <name>A0AAD5GT80_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Mediator of RNA polymerase II transcription subunit 21</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_005094</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the Mediator complex subunit 21 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005691">
        <property type="protein sequence ID" value="KAI7752424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GT80"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.280.10">
        <property type="entry name" value="Mediator complex, subunit Med21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037212">
        <property type="entry name" value="Med7/Med21-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021384">
        <property type="entry name" value="Mediator_Med21"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13381:SF0">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13381">
        <property type="entry name" value="RNA POLYMERASE II HOLOENZYME COMPONENT SRB7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11221">
        <property type="entry name" value="Med21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140718">
        <property type="entry name" value="Mediator hinge subcomplex-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 6" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="28"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="117"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="47"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="68"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU366036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7752424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="163" mass="17567" checksum="85C27843B55D6B22" modified="2024-05-29" version="1">MDIISQLQEQVNTIAAIAFNTFGTLQRDAPPVRLSPNYPDPPPAPPNANANGATPAAAVNPTTEESPNNNNNNNNNNIAETPKLLSAELVKAAKQFDALVAALPFSEGGEEAQLKKIEELQAENDLVGQELQKQLEAAEKELKQVQELFNEAADNCLNLKKPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GT86</accession>
    <name>A0AAD5GT86_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">gibberellin 2beta-dioxygenase</fullName>
            <ecNumber evidence="10">1.14.11.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_020498</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the 2-beta-hydroxylation of several biologically active gibberellins, leading to the homeostatic regulation of their endogenous level. Catabolism of gibberellins (GAs) plays a central role in plant development. Converts GA9/GA20 to GA51/GA29 and GA4/GA1 to GA34/GA8.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>gibberellin A1 + 2-oxoglutarate + O2 = gibberellin A8 + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:15005"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:58524"/>
            <dbReference type="ChEBI" id="CHEBI:58594"/>
            <dbReference type="EC" id="1.14.11.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Hormone biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Plant hormone biosynthesis; gibberellin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the iron/ascorbate-dependent oxidoreductase family. GA2OX subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.13" evidence="10"/>
    <dbReference type="EMBL" id="JAMZMK010005982">
        <property type="protein sequence ID" value="KAI7751093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GT86"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045543">
        <property type="term" value="F:gibberellin 2-beta-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.330:FF:000014">
        <property type="entry name" value="Gibberellin 2-beta-dioxygenase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050231">
        <property type="entry name" value="Iron_ascorbate_oxido_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47990">
        <property type="entry name" value="2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="5 11" id="KW-0408">Iron</keyword>
    <keyword evidence="2 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="12">
        <location>
            <begin position="166"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00052204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00055835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00061282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00066708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7751093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="330" mass="37615" checksum="C8D602B3DD6B85FB" modified="2024-05-29" version="1">MVILSQDQNKPINLANKLNKNTNFTGIPVIDLSCPDDAKNLMVKACQEYGFFKVINHGVPLWLVSELEKETMAFFNMSQPEKDKYYPPNPLGYCSKTIGLNGDTGWIEYLLFTSNNFPTNNKTFSSLAKEYVEAVRKLGCEILELMADGLKIEPKSVFSRMLSDEKADTIFRLNHYPPCPNPNQRATTNGCRNPIGFGEHTDPQLISIARSNTTSGLQICLPDGTWVPVPPDHTSFFVNVDDLLQVVTNGRFKSVRHRVVTDRLKSRISMIYFGGPPLMEKISPLRSLMDPGEESLYHEFTWFEYKSYTYRTRLAVDRLSFFHKRSHPLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GVG0</accession>
    <name>A0AAD5GVG0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BSD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_021803</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005510">
        <property type="protein sequence ID" value="KAI7753208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GVG0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3970.10">
        <property type="entry name" value="BSD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005607">
        <property type="entry name" value="BSD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035925">
        <property type="entry name" value="BSD_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31923">
        <property type="entry name" value="BSD DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31923:SF1">
        <property type="entry name" value="BSD DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03909">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00751">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140383">
        <property type="entry name" value="BSD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50858">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BSD" evidence="2">
        <location>
            <begin position="168"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="230"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="234"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="256"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="279"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7753208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="301" mass="33720" checksum="50F4DC2914D892B1" modified="2024-05-29" version="1">MDFWQKARSFAEDAAKKSQELTKEAAKRSQELTKEAAKRSQELTIGSSKLADIVSEASKRSKEIVTEASKKADLIKVEALKRADQIKAQIPSTSAAISQLVVDSSSVKASGGGDSVEDLEKFGITDDLREFVKEITINTFRDFPLEDDSEMSDIPTVSNVRQDLTKWQETHAKLVLSAVKEISKLRYELCPRVMRERKFWRIYFILVNSHVTPYEKRYKEEEENKKVVEKVNEDTTVTSAAESVSKTEASEENQKAKSTSTSTKTTDQDLDVFLLGDLGDSDEGPDDGNDDGLDDDDFDKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GVU7</accession>
    <name>A0AAD5GVU7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 18">Peroxidase</fullName>
            <ecNumber evidence="4 18">1.11.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">M8C21_020186</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">AA19_3_7</strain>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 18">
            <text>2 a phenolic donor + H2O2 = 2 a phenolic radical donor + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:56136"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:139520"/>
            <dbReference type="ChEBI" id="CHEBI:139521"/>
            <dbReference type="EC" id="1.11.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15 18">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="15 18">Binds 2 calcium ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15 18">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
        <text evidence="15 18">Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="18">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peroxidase family. Ascorbate peroxidase subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.7" evidence="4 18"/>
    <dbReference type="EMBL" id="JAMZMK010002098">
        <property type="protein sequence ID" value="KAI7755019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GVU7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140825">
        <property type="term" value="F:lactoperoxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00693">
        <property type="entry name" value="secretory_peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.420.10:FF:000006">
        <property type="entry name" value="Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.520.10:FF:000001">
        <property type="entry name" value="Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.420.10">
        <property type="entry name" value="Peroxidase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002016">
        <property type="entry name" value="Haem_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000823">
        <property type="entry name" value="Peroxidase_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019794">
        <property type="entry name" value="Peroxidases_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019793">
        <property type="entry name" value="Peroxidases_heam-ligand_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033905">
        <property type="entry name" value="Secretory_peroxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31517">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31517:SF84">
        <property type="entry name" value="PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00141">
        <property type="entry name" value="peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00458">
        <property type="entry name" value="PEROXIDASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00461">
        <property type="entry name" value="PLPEROXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00435">
        <property type="entry name" value="PEROXIDASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00436">
        <property type="entry name" value="PEROXIDASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50873">
        <property type="entry name" value="PEROXIDASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 15 18" id="KW-0106">Calcium</keyword>
    <keyword evidence="11 17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 18" id="KW-0349">Heme</keyword>
    <keyword evidence="18" id="KW-0376">Hydrogen peroxide</keyword>
    <keyword evidence="10 15 18" id="KW-0408">Iron</keyword>
    <keyword evidence="7 15 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 18" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5 18" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="18" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Plant heme peroxidase family profile" evidence="19">
        <location>
            <begin position="30"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="13">
        <location>
            <position position="71"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="15">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="16">
        <location>
            <position position="67"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="40"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="73"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="126"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="204"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU362060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAI7755019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="331" mass="35737" checksum="BBB18EFBF1067187" modified="2024-05-29" version="1">MLNMSSIILAHILTIIILVSLGCFVHSKAQLQIGFYSGSCSAAEFIVKEEVSKAYLEDRGLAAGLVRMHFHDCFVRGCDASVLIDSTPSNQAEKDSPANNPSLRGFNVIDSAKARLEVLCPGIVSCADIIAFAARDSFEITGGFGYDVPAGRRDGRVSLIAETRALPPATSSLAQLTQMFSTHGLTQDEMVTLSGAHTIGRSHCTAFASRLYNFSSTVNQDPSLNQLYASKLKQDCPKGSNNVNLVVPMNPGSPTISDTDYYVDVLNNRGLFTSDQSLLTSPSTLNQVHQHAMNPLVWKSKFASAMVKMGRIGVITGQQGEIRSNCRVINK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GY07</accession>
    <name>A0AAD5GY07_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein eL22</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">60S ribosomal protein L22</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_029487</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL22 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000178">
        <property type="protein sequence ID" value="KAI7757134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GY07"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.210:FF:000002">
        <property type="entry name" value="60S ribosomal protein L22-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002671">
        <property type="entry name" value="Ribosomal_eL22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038526">
        <property type="entry name" value="Ribosomal_eL22_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10064">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10064:SF0">
        <property type="entry name" value="FI24544P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01776">
        <property type="entry name" value="Ribosomal_L22e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00040613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00041214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7757134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="125" mass="14135" checksum="D30321AA583A4F6E" modified="2024-05-29" version="1">MSRGAAGGAKGGKKKTTSFVIDCGKPVEDKIMEIASLEKFLQERIKVGGKAGNLGDSVTISREKNKITVTADSNFSKRYLKYLTKKYLKKHNVRDWLRVIASNKDRNVYELRYFNIAENEGEEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GYC4</accession>
    <name>A0AAD5GYC4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine-containing phosphotransfer protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_021392</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Functions as a two-component phosphorelay mediators between cytokinin sensor histidine kinases and response regulators (B-type ARRs). Plays an important role in propagating cytokinin signal transduction.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="2">Histidine-containing phosphotransfer domain (HPt) contains an active histidine that mediates the phosphotransfer.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000329">
        <property type="protein sequence ID" value="KAI7756641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GYC4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009927">
        <property type="term" value="F:histidine phosphotransfer kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043424">
        <property type="term" value="F:protein histidine kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009736">
        <property type="term" value="P:cytokinin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.160">
        <property type="entry name" value="HPT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045871">
        <property type="entry name" value="AHP1-5/YPD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036641">
        <property type="entry name" value="HPT_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28242:SF30">
        <property type="entry name" value="HISTIDINE-CONTAINING PHOSPHOTRANSFER PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28242">
        <property type="entry name" value="PHOSPHORELAY INTERMEDIATE PROTEIN YPD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47226">
        <property type="entry name" value="Histidine-containing phosphotransfer domain, HPT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0932">Cytokinin signaling pathway</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0902">Two-component regulatory system</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU369004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7756641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="155" mass="17686" checksum="611E9FDF7AC5A139" modified="2024-05-29" version="1">MDNLSIEASKRLLSGHVNSMYEESDVDDVFYGYRSPRSKCSADQLAEMIKSFCVEVEREISELEGYSKLPNIDFVMTSKLARSIKARSILIGAKNVRGSCVYVIEACLVSDEETLSYAVVGLHNVFKRTKTHFETYLEMWNRIVEKEKQLAEAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GYX6</accession>
    <name>A0AAD5GYX6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Alpha/beta hydrolase fold-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_019474</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDXG' lipolytic enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000027">
        <property type="protein sequence ID" value="KAI7758109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GYX6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050466">
        <property type="entry name" value="Carboxylest/Gibb_receptor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23024:SF24">
        <property type="entry name" value="ALPHA_BETA HYDROLASE FOLD-3 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23024">
        <property type="entry name" value="ARYLACETAMIDE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="2">
        <location>
            <begin position="98"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7758109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="365" mass="41127" checksum="BBFDA80F6F8090D6" modified="2024-05-29" version="1">MEKTTTTTPTVKPSSPSLTLPWKTRILLSIISTATDAACRTDGTVNRRLLNLVDFRAPPTSKSTNGVSSHDVVVDKTRNLWFRVYVPTQHAGDDLPVIVFFHGGGFVFLAPDVMAYDAVCRRFARKLPAVVVSVNYRLAPEHRYPAQHEDCFDVLKFLDDEDNGKKALLPVNANLMRCFLVGDSAGGNLAHHVTQRACEYNFRRLKVIGVVPIQPFFGGENRMESEILLEGTPLVSLKRTDWTWKAFLPQGEEFNRDHPIINVSGPNAVDISKINFPPTMVVVAGFDALRDWQMRYYEWMKNSGKEVYLYEYPTMCHAFYIFPELPESRQLIDQVKEFVHKVSVSVSVSSNNTILGNNGKMGRSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BKD7</accession>
    <name>A0AAD5BKD7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">SAM-dependent MTase RsmB/NOP-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_006977</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012149">
        <property type="protein sequence ID" value="KAI7724863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BKD7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001510">
        <property type="term" value="P:RNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049560">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001678">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023267">
        <property type="entry name" value="RCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023269">
        <property type="entry name" value="RCMT_subfamily_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807">
        <property type="entry name" value="NOP2 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807:SF16">
        <property type="entry name" value="SAM-DEPENDENT MTASE RSMB_NOP-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01189">
        <property type="entry name" value="Methyltr_RsmB-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02010">
        <property type="entry name" value="RCMT9"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02008">
        <property type="entry name" value="RCMTFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51686">
        <property type="entry name" value="SAM_MT_RSMB_NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM-dependent MTase RsmB/NOP-type" evidence="7">
        <location>
            <begin position="50"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="454"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="533"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="466"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="289"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="590"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7724863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="590" mass="66008" checksum="5347728BF135C017" modified="2024-05-29" version="1" fragment="single">QHTEQQVRPVFSINLPSSISRKEGRKGMEAASSKLPLPDAFLQFLNDNGIDPSIYTASHFLPRYVRLRPGCEEQVEEIEADIGCKLEKVEWLPNFYSLPPQIQIAGSISYQEGKIYGIDAASGAAVSALNISTGDHVLDLCAAPVNRESGMKGDEFLYNEWTSRKPYKERKKAIKEREKGSLRLNQDPELIYYGRHSGVVGLSKTELFKSVSSHEISQYGYDKVLVDAECTHDGSIKHIQKFENWGWTTLERRVLDAERTDDLAVLQFRLLTNGFKLLKVGGYLVYSTCSLTYAQNEDIVEKFLSLNPCAELLDIDGSKCWPCKSGRIPKTLRSNEHASMALDEIRGRPDVFSYGAVWEGQELNFERVAWLKVMGIPLDLLDDSVLGNIGSFFGSVVKKPVLDWLETDSSFQYVGVVVNHGARIKDVLFLKWRGRSIKIWVEEVEKEWLSDFVDEAPQDSGGNNMETEEDGGPVEEAVPEPEVKPGLERAENMEGVNAVSNSPINEEEKLVEENLKIDSVVDPGSSVLLDDESEEMIPQPAQEKSDGDNEAVQAEVSKEQEKAATIDFGNRLGVDLQNFECLVSKTLEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BKS9</accession>
    <name>A0AAD5BKS9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CRIB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_005154</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012059">
        <property type="protein sequence ID" value="KAI7725122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BKS9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00132">
        <property type="entry name" value="CRIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.810.10">
        <property type="entry name" value="CRIB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000095">
        <property type="entry name" value="CRIB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036936">
        <property type="entry name" value="CRIB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044509">
        <property type="entry name" value="RIC2/4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46931">
        <property type="entry name" value="CRIB DOMAIN-CONTAINING PROTEIN RIC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46931:SF6">
        <property type="entry name" value="CRIB DOMAIN-CONTAINING PROTEIN RIC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50108">
        <property type="entry name" value="CRIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRIB" evidence="2">
        <location>
            <begin position="109"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="163"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="175"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7725122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="200" mass="22287" checksum="24979CBA1CF20A33" modified="2024-05-29" version="1">MKDRIERLIIFPFTAGCVSSSSVSVCLQHGKRPKEDIKSANTVCRSMEVVKEAKDTGNDEMVKDLSKFPTPSKPNIYIGFHRITRTIRSLSQSLVFKEVIEDQEMEMEIGLPTDVKHVTHIGFDGPLTHDANGCNHLAFSEFVSMCPDSMARFEVSPVVPTHVTHDTQNTSSESLDVKEPTHATHDTQNTSSESPDIKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BLA7</accession>
    <name>A0AAD5BLA7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_020242</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the peptidase A1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011941">
        <property type="protein sequence ID" value="KAI7725420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BLA7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000115">
        <property type="entry name" value="Lysosomal aspartic protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.225.10">
        <property type="entry name" value="Saposin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011001">
        <property type="entry name" value="Saposin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF80">
        <property type="entry name" value="ASPARTIC PROTEINASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47862">
        <property type="entry name" value="Saposin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="6 9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 10" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041896887" description="Peptidase A1 domain-containing protein" evidence="11">
        <location>
            <begin position="22"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="12">
        <location>
            <begin position="90"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="108"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="295"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="121"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7725420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="480" mass="51681" checksum="BA245DB3E45237E5" modified="2024-05-29" version="1" fragment="single">VNMERTLTIFLLLSTLLCARAFSTSGDGLIRVNLKKVKFDDTNRITSDLVLDEGDLLIESIRKYKQSANNLVDQQEPDIVSLKNYMDAQYYGEIGIGTPQQKFTVVFDTGSANLWVPSSKCIFSLACLVHTRYRSGQSQTYKANGTPAAIQYGTGSISGYFSQDNVQLGDLVVIDQIFIEATREPGVTFLAGKFDGILGLGFKNISVGNAVPVWENMVNQHLVKEPVFSFWLNRKSENGEGGEIVFGGVDPKHFTGEHTFVPVTQKGYWQFDMGDVIIGGKPAGFCTNGCSAIADSGTSLIAGPSEVVKQINDAIGAAGVLNEEYQKAVKLLGSHVFDQLVNSVENATQLCAHIGACPRQPGASSIGIKSVVDSSDDVSSGFEDTPSCTACKMIVSRAHSELMENETRDSILNLGSDNPLDQSIIDCAKISSMPTISFTIGGKEFQLSPDQYILKVGEGASARCISGFIALDIPPPRGPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BLI7</accession>
    <name>A0AAD5BLI7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cyanobacterial aminoacyl-tRNA synthetase CAAD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_019919</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011914">
        <property type="protein sequence ID" value="KAI7725555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BLI7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025564">
        <property type="entry name" value="CAAD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033344">
        <property type="entry name" value="CURT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33222">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33222:SF2">
        <property type="entry name" value="PROTEIN CURVATURE THYLAKOID 1D, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14159">
        <property type="entry name" value="CAAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="256"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="Cyanobacterial aminoacyl-tRNA synthetase CAAD" evidence="3">
        <location>
            <begin position="252"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7725555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="323" mass="35658" checksum="C8821FBD3D1D5E97" modified="2024-05-29" version="1" fragment="single">KIPFAKYRTLIEAAEVPQQHMLTTMELSTRCIPNGLPNFASCKPYTSSLQLKLSLPLTPNTITLNNQGERIDTFNFQGASSSFSCLVARWVVTNLGLRYYNYPIKATASEEKSGGANQYVKEEPEVVNYSSIEATTPEEASIRVNQYVEEQPDAVTYSSTEATTPEEASNVVNKYVKEEADAVNYSSIEATPEEASNGVNQYVKEETVDVVTVNEGQSDESSGFGLFKDDAAAVDQFPPDDFLNKLKVELDPENSLSIALLGVGGITALWLTTSIVGAIDHIPLFPKLMELVGLGYTIWFSTRYLLFKVCNRDCFVSFLCMLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BLQ8</accession>
    <name>A0AAD5BLQ8_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_024508</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012209">
        <property type="protein sequence ID" value="KAI7724489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BLQ8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7724489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="83" mass="9405" checksum="9386DB38F8E4A81F" modified="2024-05-29" version="1">MASTEANNVPQAHAQRDTEPVRPLANFPPSIWGDRFLSFSLDNAQLEAYAKAMEQPKEELRRLISNPAMDLNDKLSTFIKQEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BMH5</accession>
    <name>A0AAD5BMH5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">NB-ARC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_021696</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012049">
        <property type="protein sequence ID" value="KAI7725136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BMH5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058192">
        <property type="entry name" value="WHD_ROQ1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11017">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11017:SF492">
        <property type="entry name" value="TIR DOMAIN, P-LOOP CONTAINING NUCLEOSIDE TRIPHOSPHATE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23282">
        <property type="entry name" value="WHD_ROQ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NB-ARC" evidence="3">
        <location>
            <begin position="37"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein Roq1-like winged-helix" evidence="4">
        <location>
            <begin position="295"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7725136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="420" mass="47239" checksum="2BAC6EB9EDF3483B" modified="2024-05-29" version="1">MVASGPETEFIAKIVDTIDHELDLKLMSTPAHLTGMETRAEDINSWLKNEHSNTNVIAICGMGGIGKTTLAQYVYNLNKHNFESTCFLEEVGKHFKETYGVLGLQKQLLTDMLGGKNKMISCVAEGTAKINEVLQMKKVLIVLDDIDEHEELGTLLGTNVVHTQSKIIVTTRLLNIQSWFDSIFWRCHVHELTLLNDHESLEVLSCHAFGSKLPLNGFCDLAIELAGYCGGNPLALKILGSSLYVSAHDSRERNSIIEIWRSRLNLLSSFKGDLDYKIQGVLQKSFDSLPHACYKELFLHIVVFFVGEDQDFVIKILEHDLYAKVGIRTLINRCLLTVSSKNKLMMHQLLQDMGRTILREDSKDPEKHARVWCSDEAYRMLTKGDVSETIEGLTLDTRKLKKGTKVSQGGEQVCSTYEMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BMI6</accession>
    <name>A0AAD5BMI6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Auxin response factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_024779</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Auxin response factors (ARFs) are transcriptional factors that bind specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs).</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimers and heterodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the ARF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011844">
        <property type="protein sequence ID" value="KAI7725849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BMI6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009734">
        <property type="term" value="P:auxin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10017">
        <property type="entry name" value="B3_DNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.1040:FF:000001">
        <property type="entry name" value="Auxin response factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.330.10:FF:000001">
        <property type="entry name" value="Auxin response factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000047">
        <property type="entry name" value="Auxin response factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.330.10">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010525">
        <property type="entry name" value="ARF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044835">
        <property type="entry name" value="ARF_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033389">
        <property type="entry name" value="AUX/IAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003340">
        <property type="entry name" value="B3_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015300">
        <property type="entry name" value="DNA-bd_pseudobarrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053793">
        <property type="entry name" value="PB1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31384:SF79">
        <property type="entry name" value="AUXIN RESPONSE FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31384">
        <property type="entry name" value="AUXIN RESPONSE FACTOR 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06507">
        <property type="entry name" value="ARF_AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02309">
        <property type="entry name" value="AUX_IAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02362">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01019">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101936">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50863">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51745">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0927">Auxin signaling pathway</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TF-B3" evidence="11">
        <location>
            <begin position="131"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="PB1" evidence="12">
        <location>
            <begin position="697"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="368"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="384"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7725849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="831" mass="93128" checksum="39532CF3CB1CFAB4" modified="2024-05-29" version="1">MMTSSEVSSKVMDSGHGRVDEDLALYKELWRACAGPLVNVPRENDLVFYFPQGHIEQVEASTNQVADQQMPVYNLPAKILCRVLTVQLKAEPDTDEVFAQITLMPIRNQDENAVTKESAPQQEPQFRVHSFCKTLTASDTSTHGGFSVLRRHADECLPPLDMSKQPPTQELVAKDLHGSEWRFRHIFRGQPRRHLLQSGWSVFVSSKRLVAGDAFIFLRGENGELRVGVRRAMRQQPNIPSSVISSHSMHLGVLATAWHAIQTGTFFTVYYKPRTSPAEFIVPYDKYMESVKNSYSIGMRFNMRFEGEEAPEQRFTGTIVGIDERDPKMWPESKWRCLKVRWDETSSITRPERVSPWKIEPALMPPAINTLPVHRQKRPRPNVLPSSPDSSVLTREGTSKMAAAGPAPASAFSRVLQGQDLSTLRGTFMDCNESDSYDRPVQWTPSVINDDKVNPPSVSQHYGSGYGLDKLSPVGRTVGSSFTDLLSGFGTTNDSANEFSTSTSSHANPWSISPSNLSLSLLGSGTKTGLQSGEISYQTTQDVRYSAYDDYVHSNQQHERWLMPPPLPSYLHTPSHSSDLMTKPVTQNELTKPKDGNCKIFGVPLAGNRNPSDVNHGLQSQQYLAFDSDLRPEQSNHLKAVNNLPTDKEQEKDRSFQSVVRDGYVKAHGGSTRSCTKVHPQWGGVAPGRSEGPYSMDITCDVHKQGIALGRSVDLTKFNSYNELISELDELFEFGGELKCRNKNWLVVYTDDEGDMMLVGDDPWQEFCGMVRKIFIYTREEVQRMNPGTLNPRDDDTSSVGDDALMLQGAAIENRNKYSSCMKIWTNLLLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BNW7</accession>
    <name>A0AAD5BNW7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_012345</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RelA/SpoT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011499">
        <property type="protein sequence ID" value="KAI7726871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BNW7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3210.10:FF:000001">
        <property type="entry name" value="GTP pyrophosphokinase RelA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.10">
        <property type="entry name" value="Hypothetical protein af1432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43061">
        <property type="entry name" value="GTP DIPHOSPHOKINASE RSH1, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43061:SF1">
        <property type="entry name" value="GTP DIPHOSPHOKINASE RSH1, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13328">
        <property type="entry name" value="HD_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD" evidence="3">
        <location>
            <begin position="156"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="318"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7726871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="706" mass="78855" checksum="AD38498F4DDC6C3B" modified="2024-05-29" version="1" fragment="single">PVECVSVCRFSKGDGSGRYDCSVVSCAWKAPRALTGLLATTAHPCSDEDISEVLTLSVRKTSVLSNFFPPRKLLRSSLLHIGCRKWVVACSSSSPSGYDELSPEILWEVRHIVAMLEDLKPSIAYLTSNELEIVHDALKLAFEAHDGQKRRSGEPFIIHPVEVARILGELELDWESIAAGLLHDTVEDTNVVTFERIEKEFGVTVRHIVEGETKVSKLGKLKCNSEISSVQDVKAHDLRQMFLAMTEEVRVIIVKLADRLHNMRTLSHMPVHKQSSIAMETLQVFAPLAKLLGMYQIKSELENLSFMYTNPQDYGKIKRRVAELYKDHEKEIEEANRLLIKKIEDDQFLNLMALTTEVQSVCKEPYSIYKSVLKSGSSINEVNQIAQLRIIVKPKPCVGVAPLCNAQQICYHVLGLVHGFWTPIPRAVNGNLVPPLHVLANAEVVEIVTYNGLSSKSAFQRHKQWLQHAKTRSARHKIMKFLREQAALSASEITADSVNEFIADTEDDSEAEDVLDYSKGTQHTWETILMNVMEMSSSKMVDEDLFRFKNGSIKVSKVNGKHNKHMQHVSLKTNSHGNEFGKLMQANVPMYKEVLPSLESWLDGKVSSWSKLQGHSIQWLCIACIDRKGMMADITLALADAGISICSCAAEVDRGRGMAVILFHVEASLDNLVSACSRVDIVLGVLGWSTGCSWPSLGENQHIREC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BP84</accession>
    <name>A0AAD5BP84_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_030516</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011828">
        <property type="protein sequence ID" value="KAI7725918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BP84"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048306">
        <property type="term" value="F:calcium-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd16185">
        <property type="entry name" value="EFh_PEF_ALG-2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051426">
        <property type="entry name" value="Peflin/Sorcin_CaBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46212:SF3">
        <property type="entry name" value="GH27120P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46212">
        <property type="entry name" value="PEFLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="2"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="38"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7725918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="169" mass="19604" checksum="C4C36D8A1D1A6217" modified="2024-05-29" version="1">MSNMKVLKEWFNRVDSEKTGSITATQLQRALAVGNLQFPITIVQQMIRMYDFDKNGTMSFEEFVALNEFLLKVQQAFSDLERDRGFLVTDEVYEALLKLNVSMDSPAFYTVCESFDKEKNGRFRLDDFMSLCIFVQSARNLFNSFDTSKQGRVTLDLNQFIYCTANCRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BPI1</accession>
    <name>A0AAD5BPI1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_029391</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011431">
        <property type="protein sequence ID" value="KAI7727147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BPI1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01837">
        <property type="entry name" value="SGNH_plant_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051238">
        <property type="entry name" value="GDSL_esterase/lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035669">
        <property type="entry name" value="SGNH_plant_lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45650">
        <property type="entry name" value="GDSL-LIKE LIPASE/ACYLHYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45650:SF50">
        <property type="entry name" value="TRIACYLGLYCEROL LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7727147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="373" mass="41346" checksum="A7948CE6E975C449" modified="2024-05-29" version="1">MQLKYLPLQLTVKNMATTQHLVILMLTTTLLLVNSEPQVPCYFIFGDSLMDNGNNNRLHTQAKVNFPPYGIDFPDGPTGRFTNGRNAADVIAQLLGFDKFIPPFATAKGSEMLTGVNYASGAAGIRAETSEHIGARISMDNQIKNHWVTILRLSYLIGKGSIVETKKYLQKCIYTVGIGNNDYINNYFLPKYYNTSSVYTPEQYAEALVEQHSRQLQDLYKSGARMFGIFAAGYSGCTPGMMAEFGMNSCVEEVNSAVKLFNSRLNTALNYLNNRLVDAKFIFLESSLEYPNDINVTDTPCCGGSSTSSKGQCVPNQVPCSNRQNYYFWDAFHPTERINVIAGTKAYEKLTSFDAFATIGLSKDNVTGYISDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BU11</accession>
    <name>A0AAD5BU11_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_020364</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MYB-CC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010995">
        <property type="protein sequence ID" value="KAI7729457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BU11"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000002">
        <property type="entry name" value="Myb family transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025756">
        <property type="entry name" value="Myb_CC_LHEQLE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006447">
        <property type="entry name" value="Myb_dom_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046955">
        <property type="entry name" value="PHR1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01557">
        <property type="entry name" value="myb_SHAQKYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31499">
        <property type="entry name" value="MYB FAMILY TRANSCRIPTION FACTOR PHL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31499:SF85">
        <property type="entry name" value="TRANSCRIPTION FACTOR MYB-RELATED FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14379">
        <property type="entry name" value="Myb_CC_LHEQLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Myb-like" evidence="9">
        <location>
            <begin position="197"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="MYB-CC type transcription factor LHEQLE-containing" evidence="10">
        <location>
            <begin position="277"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="22"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="280"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="22"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF14379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7729457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="363" mass="41816" checksum="BB406490A8E8B61F" modified="2024-05-29" version="1">MGCNQEMVYNLKSSDYGGAQYQPWQEQEQQPQQQQQHQQRQRMISYRQQTPSCGKTIMEQIGSPASALLATERYLGFSQNGVHRSFNEKTGVSSAPQMKSSYFMQPNENNRYFSSYDRSSYKMRVINQSEKDQIRELKRKLLEESDVPDWRQSPSTICYNGNSDLGVQLGHSRPPSSQVSFNNTNPVSVVPSKTRIRWTQDLHDRFVECVNCLGGAEKATPKAILKLMDSEGLTIFHVKSHLQKYRIAKYLPESAKGNSEKRASIDTMSQIENITGMHFKDALQMQLAVQRQLHEQLEIQRNLQMRIEEQAKQLKKLFDQHQQPKDNMSSSLNDYHSINLDDHDVILNLEDDSESTLFPSKIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BVI1</accession>
    <name>A0AAD5BVI1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_019659</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011056">
        <property type="protein sequence ID" value="KAI7729156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BVI1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7729156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="122" mass="13023" checksum="0DBC89757C0028D0" modified="2024-05-29" version="1">MAIVPLCYSAPPAAHFSKLGFHQYPICRPDSSLINLHRISTSSFRFKNAPGPLIGKRLPSLHAANKPSGESNEAVTNVDTSTAQGPPFLTILAGIVVFALVIWVVGSIVSWLIGLLGLIFSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BX79</accession>
    <name>A0AAD5BX79_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">M8C21_013640</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">AA19_3_7</strain>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="11">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010596">
        <property type="protein sequence ID" value="KAI7730889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BX79"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23509">
        <property type="entry name" value="Gnk2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000162">
        <property type="entry name" value="Adenine nucleotide alpha hydrolase-like domain kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000336">
        <property type="entry name" value="Cysteine-rich receptor-like protein kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.430.20">
        <property type="entry name" value="Gnk2 domain, C-X8-C-X2-C motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052059">
        <property type="entry name" value="CR_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002902">
        <property type="entry name" value="GNK2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038408">
        <property type="entry name" value="GNK2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47973">
        <property type="entry name" value="CYSTEINE-RICH RECEPTOR-LIKE PROTEIN KINASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01657">
        <property type="entry name" value="Stress-antifung"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51473">
        <property type="entry name" value="GNK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="144"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="Gnk2-homologous" evidence="14">
        <location>
            <begin position="19"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="202"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="15">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="15">
        <location>
            <position position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAI7730889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="498" mass="56278" checksum="13B0E5B7DF03AA0D" modified="2024-05-29" version="1" fragment="single">HENSDVTFGDSDVILDDEARPIGVCSNESTSQPTETFNQVVENLLSDIRYATPRTSNYYAASTTQVPIRNTTVYAIAQCAKNVSQDICRGCLSTAYNNLLRCFPNKDGRAIDLFCFMRYSETPFFERNQSTNIKSFLSDGSSSISAMIAGVSSVAVLGLLILSFWLCYRKMKKTKTVEEVEYGLQSDKRYTYHKLRLATNDFGDEYRIGKGGFGEVFKAILDDENVVAVKRLHVGYDRAKLEFENEVRLISNVQHRNLVRQLGWCIEGPELLLVLEYIPNGSLDNVLWGERKGTLNWEKRSEIIFGVARGLSHLHNESHVKIIHRDIKLNNILLDDDFQPKICDFGLARLHPEDQTHISTKLAGTLGYMAPEYATIGHLSEKVDTYSFGIVILEIISGRRCTDQNFSGPDTSHLLEHAWQLYESGMHMELVDQELGIKEHHKANVMKTIEIALMCTQSLASLRPNMAEVVLMLSSGLTLESRKLVKPTFMDTRRVILT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BXU4</accession>
    <name>A0AAD5BXU4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cathepsin propeptide inhibitor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_011516</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010453">
        <property type="protein sequence ID" value="KAI7731405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BXU4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2250">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013201">
        <property type="entry name" value="Prot_inhib_I29"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08246">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00848">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cathepsin propeptide inhibitor" evidence="2">
        <location>
            <begin position="20"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7731405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="195" mass="22214" checksum="1CAE4987D8E6943E" modified="2024-05-29" version="1">MEGQFGAMAAASVKSDKERFAEWMKKYNRTYETKTEWEIRFSAFKENLRRVEAHFADTKLKGHWMGLGYFFDMTIRQFITEHLGCTGTGGGIVDESDSDDGEAEPEEDSEEEAEGVGKAEGDVTVKRQRIGCKDKKGVKQLQAIVDILKCFKDLKIKFLLRLVLCESSKQDVNRLIPSKQALRSTRTNTQSFIRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BYE4</accession>
    <name>A0AAD5BYE4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Amino acid transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_017489</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the amino acid/polyamine transporter 2 family. Amino acid/auxin permease (AAAP) (TC 2.A.18.2) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010659">
        <property type="protein sequence ID" value="KAI7730744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BYE4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000033">
        <property type="entry name" value="Lysine histidine transporter 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48017">
        <property type="entry name" value="OS05G0424000 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="25"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="53"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="162"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="204"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="247"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="273"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="304"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="338"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="385"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="427"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="456"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="496"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="539"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="581"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="621"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="665"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="10">
        <location>
            <begin position="91"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="10">
        <location>
            <begin position="339"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="705"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00061463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7730744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="705" mass="78649" checksum="EB51D3F2313C0862" modified="2024-05-29" version="1" fragment="single">VDNRSEKEKAIDAWLPITSSRNAKWWYAAFHNVTAMVGAGVLSLPYAMSELGWGPGVAVMVISWIVTVYTLWQMVEMHEMVPGKRFDSYSTIAWSASLDKGVQPDVQYGYKAKSTAGTVFNFLSALGDMAFAYAGHNVVLEIQATIPSTPEKPSKGPMWKGVVVAYIVIALCYFPVAFIGYWIFGNEVDDNILITLNKPTWLIAMANMFVVVHVIGSYQLYAMPVFDMMETLLVKKLNFAPSFMLRFITRNVYVAFTMFIGICIPFFGGLLGFFGGLAFAPTTYFLPCIMWLRICKPKRWSLSWFTNWICIILGVALMVVSPIGGLRSIIVQAKTYEGPGVAVLIISWVVTLYTLWQMVEMHEMVPGKRFDRYHELGQHAFGEKLGLYIVVPQQLIVEVGVCIVYMVTGGKSLQKFHKLVCIDPCKHIKLTFFIMIFASVHFVLSHLPNFNSISGVSLAAAVMSLSYSTIAWTTALDKGVQPDVEYGYKAKSTVGTVFNFFSALGDVAFAYAGHNVVLEIQATIPSTPEKPSKGPMWKGVIVAYIVVALCYFPVALIGYWIYGNAVDDNILITLNKPTWLIAMANMFVVVHVIGSYQIYAMPVFDMIETVLVKKLNFPPTFTMFIGICFPFFGGLLGFFGGFAFAPTTYFLPCIMWLSIYKPKKWSLSWITNWICIVLGVALMVVSPIGGLRSIIVQAKTYKFFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BZ93</accession>
    <name>A0AAD5BZ93_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PPPDE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_013908</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DeSI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010531">
        <property type="protein sequence ID" value="KAI7731136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BZ93"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0101005">
        <property type="term" value="F:deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1720.30">
        <property type="entry name" value="PPPDE domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008580">
        <property type="entry name" value="PPPDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042266">
        <property type="entry name" value="PPPDE_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12378">
        <property type="entry name" value="DESUMOYLATING ISOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12378:SF9">
        <property type="entry name" value="OS06G0107000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05903">
        <property type="entry name" value="Peptidase_C97"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01179">
        <property type="entry name" value="DUF862"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51858">
        <property type="entry name" value="PPPDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPPDE" evidence="4">
        <location>
            <begin position="2"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7731136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="243" mass="26288" checksum="6DC0678B3FD7DC2D" modified="2024-05-29" version="1">MTEVILHIYDVTNSDSDKTNNTIVQINKFFKDGIGLGGIFHSAVQVYGEDEWSFGFCEEGSGVFSCPFGRNPMYTYRESIVLGNTNLTKSKVNQILRELSIEWPGDCYDLLSKNCNHFCDEFCERLGVPKLPGWVNRFANAGDTAVEIAGTTAYRFRQAKTEIVTASKVAYQFLAGIASNTAAAVASDSPSSSSKGGFANPGFQQPAWFKNLVAAGAKPSSSSGIIGNGDEDIAHNLSAEIPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C273</accession>
    <name>A0AAD5C273_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Digalactosyldiacylglycerol synthase 2, chloroplastic</fullName>
            <ecNumber evidence="10">2.4.1.241</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">M8C21_028578</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">AA19_3_7</strain>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a 1,2-diacyl-3-O-(beta-D-galactosyl)-sn-glycerol + UDP-alpha-D-galactose = a 1,2-diacyl-3-O-[alpha-D-galactosyl-(1-&gt;6)-beta-D-galactosyl]-sn-glycerol + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10520"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17615"/>
            <dbReference type="ChEBI" id="CHEBI:28396"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:66914"/>
            <dbReference type="EC" id="2.4.1.241"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Plastid</location>
            <location evidence="9">Chloroplast outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 4 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.241" evidence="10"/>
    <dbReference type="EMBL" id="JAMZMK010009933">
        <property type="protein sequence ID" value="KAI7733635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C273"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009707">
        <property type="term" value="C:chloroplast outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046481">
        <property type="term" value="F:digalactosyldiacylglycerol synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019375">
        <property type="term" value="P:galactolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01635">
        <property type="entry name" value="Glycosyltransferase_GTB-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000084">
        <property type="entry name" value="Digalactosyldiacylglycerol synthase 2 chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044525">
        <property type="entry name" value="DGDG1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46132">
        <property type="entry name" value="DIGALACTOSYLDIACYLGLYCEROL SYNTHASE 2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46132:SF1">
        <property type="entry name" value="DIGALACTOSYLDIACYLGLYCEROL SYNTHASE 2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0934">Plastid</keyword>
    <keyword evidence="7" id="KW-1002">Plastid outer membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="14">
        <location>
            <begin position="217"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="444"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00071330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAI7733635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="463" mass="52693" checksum="5AF03283731ED97D" modified="2024-05-29" version="1">MDKKEHIAIFTTASLPWMTGTAVNPLFRAAYLAKDGHRKVTLVIPWLSKSDQEHVYPDKITFSSPNEHEKYVREWIEQRTELLCSFNICFYPGKFSRSKRSILALGDITECIPDEESDIAVLEEPEHLTWYHHGKRWKTKFRLVIGIVHTNYLEYVKREKNGRAYAFMLKYINNWVVDIYCHKVIRLSGATQELPRSIICNVHGVNPKFLEIGMKKKEQQQLESQAFTKGAYYIGKMVWSKGYKELLNLLQDHQNELDGLEVDLFGTGEDSDQIQEAAKELSLTIRVNPGRDHADLIFHDYKVFLNPSTTDVVCTTTAEALAMGKIVICADHVSNEFFKQFANCRTFKDGKSFVEVTRQALTEQPAPLTDSEIHELSWDAATSRFLKAAELNNIPDKKPTKSNSRSFMSSSLSFHRISEDASAYMHFVGTALVSSKPNEQQINELGLSSTSKKHGVSPQKGTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C2D3</accession>
    <name>A0AAD5C2D3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Potassium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_008936</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Potassium transporter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the HAK/KUP transporter (TC 2.A.72.3) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009903">
        <property type="protein sequence ID" value="KAI7733817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C2D3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015079">
        <property type="term" value="F:potassium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003855">
        <property type="entry name" value="K+_transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053951">
        <property type="entry name" value="K_trans_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00794">
        <property type="entry name" value="kup"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30540">
        <property type="entry name" value="OSMOTIC STRESS POTASSIUM TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30540:SF10">
        <property type="entry name" value="POTASSIUM TRANSPORTER 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02705">
        <property type="entry name" value="K_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0406">Ion transport</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0630">Potassium</keyword>
    <keyword evidence="3" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="53"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="146"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="187"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="213"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="259"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="292"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="335"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="388"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="414"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="446"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="474"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="domain" description="K+ potassium transporter integral membrane" evidence="4">
        <location>
            <begin position="16"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU321113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7733817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="676" mass="75417" checksum="D56780C1F2BCB518" modified="2024-05-29" version="1">MFMLLQKESWKTVMTLAYQSLGVVYGELSTSPLYVYKSAFAEDIHHSESNEEIFGVLSFVFWTLTLIPLLKYVFIVLRADDNGEGGTFALYTLLCRHARVSTLPNGQLADEELYEYRRDEFGSGSRDIGLSLKSTLEKHKILQKGLLVLALLGTCMVIGDGVLTPAISVFSAVSGLELSMSKEHHQYVEVPFACIILVLLFCLQHYGTHRLGFLFAPIVITWLLCISTIGIYNIFHWNPQVFQALSPYYMYKFLKKTKIQGWMSLGGILLCITGSEAMYADLGHFSQLSIKMAFTFVVYPSLILAYMGQAAYLSKHHILETDYRIGFYVSVPEKIRWPVLGIAILAAVVGSQAIITGTFSIIKQCSALGCFPRVKITHTSPTNKHGQIYIPEINWTLMLLCLAVTIGFRDTKHISNAAGLAVITVMLVTTCLMSLVMVLCWRKSVFLALGFMFFFGSIEILYFSASLIKFREGAWVPVALSCIFLVIMFVWHYGTIKKYEFDVQNKVSINWLLGLGPTLGIMRVRGIGLIHTELVSGIPAIFSHFVTNLPAIAEFIRTERADATDSNHDVREDFDNDGRMSVVGTASANLEGGIRMCEDENGFSSELQEIAVSIASPRPRKRVRFVVPESPQINTGVRDELRELMEAREAGNSRGPTNALSFPQASTLEVGMVYLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C3T1</accession>
    <name>A0AAD5C3T1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="10">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">M8C21_024537</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">AA19_3_7</strain>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the helicase family. SKI2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="10"/>
    <dbReference type="EMBL" id="JAMZMK010009738">
        <property type="protein sequence ID" value="KAI7734384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C3T1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18023">
        <property type="entry name" value="DEXHc_HFM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18795">
        <property type="entry name" value="SF2_C_Ski2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001076">
        <property type="entry name" value="ATP-dependent DNA helicase MER3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000012">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3380.10">
        <property type="entry name" value="Sec63 N-terminal domain-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052247">
        <property type="entry name" value="Meiotic_Crossover_Helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004179">
        <property type="entry name" value="Sec63-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057842">
        <property type="entry name" value="WH_MER3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47835:SF3">
        <property type="entry name" value="HELICASE FOR MEIOSIS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47835">
        <property type="entry name" value="HFM1, ATP DEPENDENT DNA HELICASE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02889">
        <property type="entry name" value="Sec63"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23445">
        <property type="entry name" value="WHD_SNRNP200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00973">
        <property type="entry name" value="Sec63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158702">
        <property type="entry name" value="Sec63 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-0469">Meiosis</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="244"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="13">
        <location>
            <begin position="34"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="14">
        <location>
            <begin position="279"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAI7734384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="774" mass="87836" checksum="662F56157A08D5A6" modified="2024-05-29" version="1">MDSYSLKSVSDLPDPFRSTFTFRYFNSLQSECFSACFHSDINMVISAPTGSGKTVLFELCILRLLSKFFSPEGKFVHTKGTLKTIYISPSKALVQEKLRSWNQKLGSWGINCLELTGDNESYNIRNIQEADIVLTTPEKFDSVTRFGIKDGGLSFFSDISLVLIDEVHLLNDPRGAALEAVVSRIKMIGRSPQMKTSPLAHVRFLAVSATIPNIDDLAEWLMVPTQGIKRFGEEMRPVKLTTKFLAVLLFGIYLMLKFLLAGYTPAKNDFLFEKRLQNYLFDLLMQYSSRKSALVFCSTRKGAQEAAQVLSQTAMTHGYSNPFIKSREQQERLREASLSCGDKQMQSYILYGGKFGYHNGGLSPKDRSLIEGLFLNGDLQVLCTTNTLAHGINLPAHTVIIKSTQYYNKEKGIYTEYDRSMILQMCGRAGRPPFDDTGTVIIMTRKETAHLYENLLGGCELVESQLLPCVTEHLAAEIVQLTVPDITRAIEWMKCSYLYVRMKKNPQNYAIKKGLYGTHIEKHMQEVCVQKVNELSDYQMIWTDEDGFVLKPLEPGRLMTKYYLKFDTMKHIMKAPLNCSIEDALHIVCRAEEISWLHITVSHIQDMNSVCSNGCRIAKCMKEYFIYKKNYKGALNSILLAKSLHQKLWDDSPYLLKQLSGIGMVTAKALQSMGVKTFDSLSEADPRKIEMVTGRKFPFGNHIKESLLSLPPKVDMKVEEITCPNYGKSKLAVTLTRLSQSPQTKRHYADMLVAIEEDNLIVFHEKIRVEEFTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C4L5</accession>
    <name>A0AAD5C4L5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">LOB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_004961</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009569">
        <property type="protein sequence ID" value="KAI7735092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C4L5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00428">
        <property type="entry name" value="Ribosomal_60s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="121"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7735092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="133" mass="15080" checksum="455CEC534EBDBD4A" modified="2024-05-29" version="1" fragment="single">MNLFPMLSTETRCAGCMVLNEPCFVNCPYRNVFPYDKKGVDDFILIHDWMNIRHVRITLLRLFNDTEQQKVYVESLLDSIRNIENTFLKPEGDHAEKIATAAAASGGAAAPPPQEKKEEPKEESDDDDDDDGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C5B2</accession>
    <name>A0AAD5C5B2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_029732</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009504">
        <property type="protein sequence ID" value="KAI7735315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C5B2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009640">
        <property type="term" value="P:photomorphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044630">
        <property type="entry name" value="SPA1/2/3/4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44218:SF8">
        <property type="entry name" value="COATOMER BETA' SUBUNIT (COPB2)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44218">
        <property type="entry name" value="PROTEIN SPA1-RELATED 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="134"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="220"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7735315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="354" mass="39513" checksum="FBD808965BDD4501" modified="2024-05-29" version="1" fragment="single">MNLRNWLEHEVVNNKLERLQIYRRVVQVVDMMHSQGNALQDLRLSNFILLPSDQIFYFSSTSTDSSSSSVTAVLERSWYACPEEVCSVRDLFSANVYSLGILLFEYLKNYLASADYDGLIQVWDACTGQGLSHYIEHQRRAWCVDFSHVDPMQFASGSDDCSVKLWSLNNKTPTCTIRSGANVCCVQFSSCSSHMLAFGSADYKIRCYDLRHTRIPWCTLADHQKAVSYVKFMGSDSLVSSSTDNTLKLWDLKKTSLEGTSTDACSMTYKGHSNEKNFVGLSVLDGYIACGSESNEVFAYHKSFPMPITSYKFGCSDSVSGDEAVDSNGHFVSSVCWREKSNMIVAANSGGSVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C7F6</accession>
    <name>A0AAD5C7F6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_006729</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009405">
        <property type="protein sequence ID" value="KAI7735938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C7F6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7735938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="128" mass="15156" checksum="363F15C3BE1358F0" modified="2024-05-29" version="1" fragment="single">TVHFEGKRVFVRELPSYERERERERERERERERTLLYQSPSRFHPNEANPFVLFTKDLTARVDYVLRIASRKPTLNEEAHGTRGLHDGNPSDKSNANEATYKLLYCEVKEMRIDCSKTFKWDGKPLGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C7V3</accession>
    <name>A0AAD5C7V3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rab3GAP catalytic subunit conserved domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_030754</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009463">
        <property type="protein sequence ID" value="KAI7735591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C7V3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045700">
        <property type="entry name" value="Rab3GAP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026147">
        <property type="entry name" value="Rab3GAP1_conserved"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21422">
        <property type="entry name" value="RAB3 GTPASE-ACTIVATING PROTEIN CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21422:SF17">
        <property type="entry name" value="RAB3 GTPASE-ACTIVATING PROTEIN CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13890">
        <property type="entry name" value="Rab3-GTPase_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab3GAP catalytic subunit conserved" evidence="2">
        <location>
            <begin position="272"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="357"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7735591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="486" mass="54655" checksum="A62637EB7E9F55CC" modified="2024-05-29" version="1">MAFPIFDGHVIGIISYNLSPRTPNSKEKNDDSPAPDDVNSDIIPSASMLKQLAIGVETTKVVKSMKDLMILAKDYSPVKERSGLSLSAMKSFVVGEKEDKFAAEFGCDEKVMSFIDLLTDPEGYLLRRKNRSRLDTPTRMTKLSKELHGAPPESFVPELAEAMGSLKTLRKMALFWSRVVAELRRLWCEGKHIPGIPLDDIPNLNSCLLHQQLQVINCCISRKRRRAIATASLDFVLKQANNIAELSHPPVDLLPESHHVYARISSGEFVLRLGADKQLENTTLLDTGEPVYTPVMQEWPLLTEDIIKETEEFVLRTGSVGAACSQLLSDMQAFKAANPGCILEDFVRWHSPPDWTEAYDESKETTGGDTSPSRGHLSTRMQKEGNLWRELWETAKPVPAVRQSPLYDEDLSVEGILHGLENFSPSSLFEQLFLSLDVQLLRPESRVMNFCTRCSKSAETMLSLFVKAKVGLRKPTIYARYMRRWP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C8G8</accession>
    <name>A0AAD5C8G8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Bet v I/Major latex protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_003676</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BetVI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009394">
        <property type="protein sequence ID" value="KAI7736024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C8G8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010427">
        <property type="term" value="F:abscisic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004864">
        <property type="term" value="F:protein phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009738">
        <property type="term" value="P:abscisic acid-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07816">
        <property type="entry name" value="Bet_v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.530.20:FF:000007">
        <property type="entry name" value="Major pollen allergen Bet v 1-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000916">
        <property type="entry name" value="Bet_v_I/MLP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024949">
        <property type="entry name" value="Bet_v_I_allergen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050279">
        <property type="entry name" value="Plant_def-hormone_signal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31213">
        <property type="entry name" value="OS08G0374000 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31213:SF55">
        <property type="entry name" value="STRESS-INDUCED PROTEIN SAM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00407">
        <property type="entry name" value="Bet_v_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00634">
        <property type="entry name" value="BETALLERGEN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01037">
        <property type="entry name" value="Bet_v_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bet v I/Major latex protein" evidence="2">
        <location>
            <begin position="1"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7736024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="155" mass="17090" checksum="141837C9D5CBDE04" modified="2024-05-29" version="1">MTSVTFEIEVASQLPADKVFKVFADLDNLGPKLHPQYFKSIETIEGNGDVGTIKLFTFGDGIPFTSGKYKTESVDVSKYSYSYSVIEGDVLMGILDSINHDVKVVPSADGGSVFKQTVVYNCKGSEKPSEEFLHKEKDVYINIFKTLEAYAIANP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C9A7</accession>
    <name>A0AAD5C9A7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Sugar phosphate transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_011875</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008969">
        <property type="protein sequence ID" value="KAI7737703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C9A7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004853">
        <property type="entry name" value="Sugar_P_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050186">
        <property type="entry name" value="TPT_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11132">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03151">
        <property type="entry name" value="TPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="98"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="127"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="239"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="270"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="346"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="Sugar phosphate transporter" evidence="6">
        <location>
            <begin position="96"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7737703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="370" mass="40590" checksum="62E06BBFBB8C4BE4" modified="2024-05-29" version="1">MIISLKQPITTTPKHTFKTINHTTISPSKLFLSTPKELKKLSVFTNSKPLHVSLIQSFGSLKKFESCVVCKAYEADRSDGDFIINMEQKTEAAKRLKIGFYFATWWLLNVIFNIYNKKVLNAFPYPWLTSTLSLAAGSAIMLVSWGAKVAEAPTTDIEFWKALFPVALAHTIGHVAATVSMSKVAVSFTHIIKSGEPAFSVLVSRFILGETFPTSVYLSLLPIIGGCGLAALTELNFNMTGFMGAMISNLAFVFRNIFSKRGMKGKSVSGMNYYACLSMLSLLILTPFAIAVEGPKMWAAGWQNAVTEIVSYMSLDEISPLTFSIGNTMKRISVIVSSIIIFHTPVQPINALGAAIAIFGTFLYSQSKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C9N7</accession>
    <name>A0AAD5C9N7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_021177</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009092">
        <property type="protein sequence ID" value="KAI7737198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C9N7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7737198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="47" mass="5321" checksum="51BA7F6102B43B78" modified="2024-05-29" version="1">MDNCRELKDDPGTGYNDGGRRAGFKWSTFDLDVLLSLNNVRNMLTDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CAA7</accession>
    <name>A0AAD5CAA7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Metal-nicotianamine transporter YSL7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_014546</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the YSL (TC 2.A.67.2) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008871">
        <property type="protein sequence ID" value="KAI7738137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CAA7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051980">
        <property type="term" value="F:iron-nicotianamine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035673">
        <property type="term" value="F:oligopeptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010039">
        <property type="term" value="P:response to iron ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048316">
        <property type="term" value="P:seed development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004813">
        <property type="entry name" value="OPT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045035">
        <property type="entry name" value="YSL-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00728">
        <property type="entry name" value="OPT_sfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31645:SF11">
        <property type="entry name" value="METAL-NICOTIANAMINE TRANSPORTER YSL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31645">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER YGL114W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03169">
        <property type="entry name" value="OPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="75"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="99"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="147"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="187"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="290"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="353"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7738137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="416" mass="46912" checksum="FC03F1FF1C38C157" modified="2024-05-29" version="1" fragment="single">FLECNISQLTSDCDLTPSILSFIEDIRSYKSMRNIEMKESGEIERDMEEIEQEKKDDDFDSLQVSKRLQPWTKQITVRGVVASIVLGSIYTVIAMKLNLTTGILPNLNVSAALLAFVYMKSWTKLLQKSGISTVPFTRHENTMIQTCSVACYSIAIGGGFGSYILALNKKTYELAGGANSPGTYKELHVGWLIGYSFLSCFAGLFVLIPLRKILIVDYKLIFPSGTATAVLINGFHSQGDKTAKKQVKGFSKYFSISFLWAIFQWFYTGQGECGFAQFPTFGLQAWKRKFYFDFSLTYVGTGMICPHIVSFSLLFGAVISWGIMWPLIEKQKGEWFPSNVPESSMESINGYKVFISIALLLGDGLYNFIKILYFTYASIYGKLKRENLNLARMNLQKMNEYKTKFSLKTAYPCGSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CDJ7</accession>
    <name>A0AAD5CDJ7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_010828</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008804">
        <property type="protein sequence ID" value="KAI7738501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CDJ7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000181">
        <property type="entry name" value="CALML5 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000235">
        <property type="entry name" value="Probable calcium-binding protein CML15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050145">
        <property type="entry name" value="Centrin_CML-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23050">
        <property type="entry name" value="CALCIUM BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="8"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="44"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="84"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="120"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7738501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="164" mass="18188" checksum="3FF6BE3FEC73B6B4" modified="2024-05-29" version="1">MSKLMAVDQRNQLRDIFNRFDMDSDGSLTHLELAALLRSLGLEPSGDQIYMLFNKMDSNGNGAVEFDELVNAMSPNMMTEQNVINQKLLLKVFHLFDRDGNGFISPSELTHAMAKMGQPLTYRELSEMIEEADTDGDGVISFSEFARVMAKSAAESFGSPVPSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CFS9</accession>
    <name>A0AAD5CFS9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">adenine phosphoribosyltransferase</fullName>
            <ecNumber evidence="6">2.4.2.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_014721</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>AMP + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + adenine</text>
            <dbReference type="Rhea" id="RHEA:16609"/>
            <dbReference type="ChEBI" id="CHEBI:16708"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.4.2.7"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the purine/pyrimidine phosphoribosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.7" evidence="6"/>
    <dbReference type="EMBL" id="JAMZMK010008362">
        <property type="protein sequence ID" value="KAI7740699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CFS9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003999">
        <property type="term" value="F:adenine phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006168">
        <property type="term" value="P:adenine salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000022">
        <property type="entry name" value="Adenine phosphoribosyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00004">
        <property type="entry name" value="Aden_phosphoribosyltr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005764">
        <property type="entry name" value="Ade_phspho_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050120">
        <property type="entry name" value="Adenine_PRTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01090">
        <property type="entry name" value="apt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002634">
        <property type="entry name" value="PRK02304.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002636">
        <property type="entry name" value="PRK02304.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11776">
        <property type="entry name" value="ADENINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11776:SF33">
        <property type="entry name" value="ADENINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10" id="KW-0660">Purine salvage</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="11">
        <location>
            <begin position="43"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7740699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="191" mass="20836" checksum="ED884906B7EB6201" modified="2024-05-29" version="1">MSDGENNGSADRIKAIKSTLRVVPDFPKPGMLFLDITTLLLDPKAFKDTIDLFVERYKQKDISVVAGIEARGFMFGPAIALAIGAKFVPLRKPKKLPGEVISEKYVLEYGTDCLEMHVGAVERGERALVVDDLVATGGTLCAAMNLLERAGAMVVECACVVEVPDLKGRERLRLHGTPLYVLIESQFEPTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CFY4</accession>
    <name>A0AAD5CFY4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_019361</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008252">
        <property type="protein sequence ID" value="KAI7741182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CFY4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051959">
        <property type="entry name" value="PAK1-Kinase_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44675:SF1">
        <property type="entry name" value="P21-ACTIVATED PROTEIN KINASE-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44675">
        <property type="entry name" value="PAK1 INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="64"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="111"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="120"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7741182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="130" mass="14015" checksum="BC6911B375024948" modified="2024-05-29" version="1" fragment="single">TRVARLSVGRNGLVYTGGEDRNLTAWDTVSGKVAYCIEDAHSARLKGIVVLSKIDETSDEEPFLVASASSDGIIRVWDVRMAKKDKPTPLAEANTKSRLTCLAGSSVKSLKKRFGGSSDSNKEQDEATEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CG82</accession>
    <name>A0AAD5CG82_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Protein transport protein Sec61 subunit gamma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_000821</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Necessary for protein translocation in the endoplasmic reticulum.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SecE/SEC61-gamma family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008207">
        <property type="protein sequence ID" value="KAI7741361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CG82"/>
    <dbReference type="SMR" id="A0AAD5CG82"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.820:FF:000001">
        <property type="entry name" value="Transport protein Sec61 subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.820">
        <property type="entry name" value="Preprotein translocase SecE subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00422">
        <property type="entry name" value="SecE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023391">
        <property type="entry name" value="Prot_translocase_SecE_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008158">
        <property type="entry name" value="Translocase_Sec61-g"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001901">
        <property type="entry name" value="Translocase_SecE/Sec61-g"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00327">
        <property type="entry name" value="secE_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12309">
        <property type="entry name" value="SEC61 GAMMA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00584">
        <property type="entry name" value="SecE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103456">
        <property type="entry name" value="Preprotein translocase SecE subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01067">
        <property type="entry name" value="SECE_SEC61G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="36"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00055400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7741361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="69" mass="7879" checksum="5E4D47ECB6BC6877" modified="2024-05-29" version="1">MDALDSVFEPLREFSKDSYRLVKRCHKPDRKEFSKVAVRTAIGFVVMGFVGFFVKLIFIPINNIIVGSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CGC7</accession>
    <name>A0AAD5CGC7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein SPIRAL1-like 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_025777</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SPIRAL1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008531">
        <property type="protein sequence ID" value="KAI7740029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CGC7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010005">
        <property type="term" value="C:cortical microtubule, transverse to long axis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043622">
        <property type="term" value="P:cortical microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039613">
        <property type="entry name" value="SPR1/2/3/4/5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33403:SF48">
        <property type="entry name" value="PROTEIN SPIRAL1-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33403">
        <property type="entry name" value="SPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0493">Microtubule</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="69"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7740029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="133" mass="13678" checksum="A9C74F03AFB47D57" modified="2024-05-29" version="1">MGRGVSCGGGQSSLGYLFGSGGEESAVNTQPPPQIVNEEAAAAATTVPTSESLEPCGVSATTETDESSQKTPESFNIINQNPSPATHNSTTNNYHRADGQNCGNFITDRPSTKVHAAPGGNSSLGYLFGDDKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CIX7</accession>
    <name>A0AAD5CIX7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_031333</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007857">
        <property type="protein sequence ID" value="KAI7742908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CIX7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10747">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.260.20:FF:000030">
        <property type="entry name" value="DNAJ heat shock family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.260.20:FF:000002">
        <property type="entry name" value="Dnaj homolog subfamily b member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.260.20">
        <property type="entry name" value="Urease metallochaperone UreE, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002939">
        <property type="entry name" value="DnaJ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051339">
        <property type="entry name" value="DnaJ_subfamily_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008971">
        <property type="entry name" value="HSP40/DnaJ_pept-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24078:SF175">
        <property type="entry name" value="DNAJ HEAT SHOCK FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24078">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01556">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49493">
        <property type="entry name" value="HSP40/DnaJ peptide-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="3">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7742908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="319" mass="35783" checksum="3E8DAAAE8DE720A7" modified="2024-05-29" version="1">MKWHPDKNINIASSGNDDDKHKVAESKFKQISEAYYVLSDPKKRQIYDMYGEEGLKSGVYDDDALSSASFNSVMKSGSGFGFRFDPRDADEIFNEFFYGSGDDGGRRRKNNNNINYNNNINKNINNNNNQKSVRKAETVENKLSCSLEELYKGSKRKMQISRIVLDDSGKPGTLEEILTIHIKPGWKKGTKITFPEKGNQEPGAAPGDLIFVIDEKPHDTFKRDGNDLVFTKRITLLDALTGKTIKVTTLDGRNLAIPITEVIKPGHEEVVQNEGMPISKEPGKKGNLRIKIDIKFPSRLTGEQKSDLKRVLGGRVVDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CJD0</accession>
    <name>A0AAD5CJD0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Kinesin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_017630</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. KIN-12 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008155">
        <property type="protein sequence ID" value="KAI7741621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CJD0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044986">
        <property type="entry name" value="KIF15/KIN-12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37739:SF16">
        <property type="entry name" value="KINESIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37739">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIN-12D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0493">Microtubule</keyword>
    <keyword evidence="5" id="KW-0505">Motor protein</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="10">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="101"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="25"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="148"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7741621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="178" mass="19877" checksum="3EDE73C9B5B96A64" modified="2024-05-29" version="1" fragment="single">CKNETFSTLRFAQRAKAIKNKAVVNEKLQNDVNTLREVIRQLKDELQRMKSTGNQVDPNAGSSSGWNARRSINLLKFSLNHPMILPHIDDDGDEEMEIVEESEPILDRKDSEDTDVNMQEDISDQVGNLEVTAQNLENNEDKKDSLKDASTQNGLGDCVNTNLPPEPSEASLVLKSPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CJQ9</accession>
    <name>A0AAD5CJQ9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_007616</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007977">
        <property type="protein sequence ID" value="KAI7742455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CJQ9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22117">
        <property type="entry name" value="F-box_FBXL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044260">
        <property type="entry name" value="SKIP8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037401">
        <property type="entry name" value="SnoaL-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47124">
        <property type="entry name" value="F-BOX PROTEIN SKIP8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47124:SF1">
        <property type="entry name" value="F-BOX PROTEIN SKIP8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13474">
        <property type="entry name" value="SnoaL_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="91"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7742455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="294" mass="32982" checksum="E716C2F8257F1519" modified="2024-05-29" version="1">MDILSSLSNNGDTPIFLALIVTILCCFFSLFFRLLKYPQSDKTNPCNCASSDSSMAVTATARLMNGGGGGGGQVPVVERERERERQHVIGASMMEQLVPEITTHALSYLDYPSLCRLSMTNSSMRRAANDDNAWKALYHKDFTMEQDSITPANGWKAYYAATRAIVNINQHFFDIIRERSLPEMGRLWLNADYVKCIHASGEFFTGYSGVLSSWQLALNWEPAVNFQVRDVRSRVLPGVAWVTMKAYVGMEHNTHPMSMTNVFELHNGEWFMVHHHSSVMLADGEVADQPPMLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CK15</accession>
    <name>A0AAD5CK15_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_019952</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the iron/ascorbate-dependent oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007834">
        <property type="protein sequence ID" value="KAI7743047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CK15"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050295">
        <property type="entry name" value="Plant_2OG-oxidoreductases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47991">
        <property type="entry name" value="OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0408">Iron</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="5">
        <location>
            <begin position="151"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7743047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="300" mass="34107" checksum="CC1326B4F4B407D6" modified="2024-05-29" version="1">MATITKRLLLSDLVSTDNIDQVPSNYIRPITQRPNLQNVVHDSIPLINLDQLFGPNRAKIIEQIGLACREYGFFQVKNHSVPESTISNMMQIARDFFNLPEQERLKNYSDDPTKTTRLSTSFNIRTEKLIEAISESLGLDKDYISGQLGNHAQHMALNYYPPCPQPDLTYGLPGHTDLNLITILLQDEVPGLQVLRNGHWVAVDPVPNTFIINIGDQIQVMSNDKYKSVLHRAVVNCDKERISIPTFYCPSPDAIIRPAPELLTDDEPAVYKQFTYGEYYEKFWNRGLATESCLDMFMAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CK23</accession>
    <name>A0AAD5CK23_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Sn1-specific diacylglycerol lipase alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_032659</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007835">
        <property type="protein sequence ID" value="KAI7742994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CK23"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00519">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002921">
        <property type="entry name" value="Fungal_lipase-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005592">
        <property type="entry name" value="Mono/diacylglycerol_lipase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46023:SF6">
        <property type="entry name" value="LIPASE CLASS 3 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46023">
        <property type="entry name" value="LIPASE CLASS 3 PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03893">
        <property type="entry name" value="Lipase3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01764">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mono-/di-acylglycerol lipase N-terminal" evidence="4">
        <location>
            <begin position="52"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="Fungal lipase-type" evidence="3">
        <location>
            <begin position="185"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="437"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7742994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="633" mass="69795" checksum="B6DA24DA2BD9C5E7" modified="2024-05-29" version="1">MAATTMATAAGAVVLLYYVLVRRMTSADAKAVEDDEGREYMKSSKSKRKIAQRRPAQAPATWLETISTLSETLRFTYSETLGKWPIGDLAFGINYLLRRQGNVQVASVYAGENSIQLKGPEIVAELKYYLQLLTICILFSKKPFPVFLESAGYSEADVLLQKPKAGILKPAFTIICDKKSKCFLLLIRGTHSIKDTLTAATGGVVPFHHSVLHDGGISNLILGYAHCGMVAAARWIAKLSIPFLLESLKENPGFEVKIVGHSLGGGTAALLTYILREHKDLSSISCITFAPAACMTWELAESGKHFITTIINGSDLVPTFSTASIDDLRTEVTASSWLNDLRDQVEQTRVLNVVFRSATALGSRLPSIANARAKVAGAGAMLRPVVMKRAQNVAQAVVRTRSTLSAWSCMGARRRAVGIIPTDNFSETSLILEKNPDSLATDTNHPAHEPNMNHGSSSDGSGSGHDDTDEEELLIPVDHVTSKSEHITEGELLYELEKELQRQENEDHIQAQKEEEAAAEEIIEEEKVIADSVDHEQPISSSDMVESHHLYPPGRIMHIVSTISNDPVDLGNDHNGTIEEHADDHEHENEHVGIYETPRDLYGKLRLSRTMINDHYMPMYKKMMEKLLTELEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CK27</accession>
    <name>A0AAD5CK27_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_020965</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007715">
        <property type="protein sequence ID" value="KAI7743523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CK27"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080147">
        <property type="term" value="P:root hair cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000017">
        <property type="entry name" value="Transcription factor bHLH66"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045843">
        <property type="entry name" value="IND-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16223:SF268">
        <property type="entry name" value="SPERMATOGENESIS- AND OOGENESIS-SPECIFIC BASIC HELIX-LOOP-HELIX-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16223">
        <property type="entry name" value="TRANSCRIPTION FACTOR BHLH83-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="7">
        <location>
            <begin position="187"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="128"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="261"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="328"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="150"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="271"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="347"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7743523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="381" mass="40203" checksum="9A15450FCF13A66D" modified="2024-05-29" version="1">MSQTFDPTSSHEDFLEQMLCGLQSAGVSWPEISTGTVAGGGDGVDLFDDQSAFLTTKLRQHQISGGDGAAGDLSSAAKSLLLQQQLMLAGDLGFFQNEMVDTSSFKSPNGNNSMQALFNGFTGSLQANQTPNFQYPPAQSFRPSGPATAPVNQRPTTVGATTPTAQGGASSGPPAQPRQKVRARRGQATDPHSIAERLRRERIAERMKSLQELVPNANKTDKASMLDEIIDYVKFLQLQVKVLSMSRMGGVAAVAPLVTDSTSCEGGRDNAQAQPSSSNDTMTVAENQVVKLMEEDMGSAMQYLQGKGLCLMPISLATAISTVTTTSHHSSATNNRPLLGGEGIIPSSPNLSRMSVQSANGHVLPQEATSVKDSNGAVKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CK44</accession>
    <name>A0AAD5CK44_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phospholipase/carboxylesterase/thioesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_030717</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007749">
        <property type="protein sequence ID" value="KAI7743303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CK44"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003140">
        <property type="entry name" value="PLipase/COase/thioEstase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46234">
        <property type="entry name" value="ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02230">
        <property type="entry name" value="Abhydrolase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phospholipase/carboxylesterase/thioesterase" evidence="1">
        <location>
            <begin position="25"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7743303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="253" mass="27508" checksum="445E986569AD3FE6" modified="2024-05-29" version="1">MSYNSSNRDSGSRSVRRNVHDFGRTFVVMPKETHTATIVWLHGIGEKGSSWTQILETFPLPNVKWICPSAPSRLLSLYGGYPCTAWYNITSMSEDACDDLEGLDASATHVANLLISEPDDVKLGVAGFSMGASVALYSAACRAIGQYGNGNRYPINLSAAVSLSGWLPCSRNLRNRVGASQEAARRASSLPVLFCHGQVDDVVEYKLGEKSAQTMYSAGFQNLTFQTYNGLGHYTILEEINDVCCWLTACLGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CKX8</accession>
    <name>A0AAD5CKX8_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_000741</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007713">
        <property type="protein sequence ID" value="KAI7743542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CKX8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005884">
        <property type="term" value="C:actin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008093">
        <property type="term" value="F:cytoskeletal anchor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051764">
        <property type="term" value="P:actin crosslink formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46756:SF18">
        <property type="entry name" value="GAS2-LIKE PROTEIN PICKLED EGGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46756">
        <property type="entry name" value="TRANSGELIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="316"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="359"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="97"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="320"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="364"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7743542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="460" mass="51676" checksum="F19527C7102B7F5A" modified="2024-05-29" version="1">MLLVKYMELKNYKARMFVPVDTRKSIGRYRPYSNVCKVMGLSGVDLFSPSDVVEKRNTRKVPDFDIVTSTVAMPTDAVRCIRRSLELSSMSNVTTGQRKHASLKSRQKTSFASDIQEEEPYLEESDEENSQFSETSYVDFLYLETGESPDTADKCALTQSIQQSDASNRELSKLAHSPVSVLLQYVNSENQLDETLSMCRVWRKLPHNAESSPTSSVLGRVLDFDFDAKSESDDLATLESATYDKLSGLCNQPELQDLERSSDSFSQWGDEYDTKSCCSVDQDHESLNKTEKDLGHSLVSVKDDVTNIADSMQESVAGKESNDKLMKIDDPKSTDNINDVFDDPGGVKFTERYDSCAEDSNTASKDERLDDSLAKADTDEKNDTRAFNTEKKLSYVAPLLKTVAKGTALIGILFLLHFRNSRSPRNTSQIHWKSIGVGLPKRKEDKGSKIYPVDKFKFED</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CL09</accession>
    <name>A0AAD5CL09_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_000786</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007713">
        <property type="protein sequence ID" value="KAI7743587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CL09"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000502">
        <property type="term" value="C:proteasome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007292">
        <property type="term" value="P:female gamete generation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010078">
        <property type="term" value="P:maintenance of root meristem identity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048232">
        <property type="term" value="P:male gamete generation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000067">
        <property type="entry name" value="26S protease regulatory subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000007">
        <property type="entry name" value="26S proteasome regulatory subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000039">
        <property type="entry name" value="26S proteasome regulatory subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050221">
        <property type="entry name" value="26S_Proteasome_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032501">
        <property type="entry name" value="Prot_ATP_ID_OB_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23073">
        <property type="entry name" value="26S PROTEASOME REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16450">
        <property type="entry name" value="Prot_ATP_ID_OB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0647">Proteasome</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="11">
        <location>
            <begin position="222"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="88"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="16"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="88"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7743587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="444" mass="49558" checksum="80833FF6D4C2E805" modified="2024-05-29" version="1">MGQGTPGGFSRQNNSGDRKDGDKKEKKFEPAAPPARVGRKQRKQKGPEAAARLPTVTPHSKCRLRLLKLERIKDYLLMEEEFVANQERLKPQEEKTEEDRSKVDDLRGSPMSVGNLEELIDENHAIVSSSIGPEYYVGILSFVDKDQLEPGCAILMHNKVLSVVGLLQDDVDPMVSVMKVEKAPLESYADIGGLDAQIQEIKEAVELPLTHPELYEDIGIKPPKGVILYGEPGTGKTLLAKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTKRYDAHSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNKIESLDPALLRPGRIDRKIEFPLPDIKTRRRIFQIHTSRMTLADDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMKVTHPDFKKAKDKVMFKKKEGVPEGLYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CNL8</accession>
    <name>A0AAD5CNL8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2 NT-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_018094</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007315">
        <property type="protein sequence ID" value="KAI7745253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019448">
        <property type="entry name" value="NT-C2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34452">
        <property type="entry name" value="MYOSIN HEAVY CHAIN-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34452:SF1">
        <property type="entry name" value="SPORULATION-SPECIFIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10358">
        <property type="entry name" value="NT-C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51840">
        <property type="entry name" value="C2_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2 NT-type" evidence="3">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="158"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="259"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="379"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="566"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="674"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="777"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="858"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="910"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1053"/>
            <end position="1108"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1147"/>
            <end position="1200"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1291"/>
            <end position="1325"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7745253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="1364" mass="155869" checksum="B4902C7FF7070620" modified="2024-05-29" version="1" fragment="single">LHFASVASSPSQIHPSLLGFWIRIVLSTKLKKIPQHGWEKLFISFIPTETGKATAKTSKANVRGGSCKWADPIYETTRLLLDSKTKQYDDKLYKLVVGMGTSRSSILGEATINLADYADASQPAILSLPLHGSDHGTTLHVTVQLLTAKTGFREFEQQRDKGLQSGNNVNKETEHSTAKSPSSELQIPDDLVNKAKTRIQLREETSIHGEYSDSQVTCDESSNASDCYYADKHDSPAEKSSSSDQAKLDNDVAVVYEENHRLRASLAAAESSISELKQELTALQTHANEMGTETQKIAQELTLEIASGQELIKEVNLLKSECSKLKNDLQNLIDIKSSSVVKERKLEKIEHNKEVNGVLLVESQIRQIQEKILIGESDLSRLQSDFEVLLGTVQDLKNETFGEVQIQPLQELETTKAEKENLVKKMDQMECYYESLVQELEENQKRILGDCQNLKTEMNDLSSVNNELQKRVTVSESALKRARLNYSIAVSQLQKDLDMLSFQVLSMFETNQTVIKEAFSDTSQLSSKHLSNDLKTSLSLQENIYKKVEEELGEMCSANLQLNVYSKTLEETLFEASDHIRKLNEKLQELGKDLQFSNQSRDVLFQENGYYLAKCNDLQLQNQMLESDLGNLSNENFLLMEKITECEALLMDYRVYKGKYEVVSSEKLELEKLLEVEVFDKQNLQKEIKNLNSEMDDVKMKFMNGLYQAVAKMEIPKPSFEKLQFQIDSVANMFQSSLESGERHVQNSEVMLKNLSSLELELQRISSEDKSFHEQIISELKKENQDLISYVENKREELDRLQECLDAERGTVARLTSELESQRSELESFKLKLDASNAHNAEILKSNDLLETTLANVNNEHDLEVRQLKDTLTKSKAEVEQFKELQMKLDDTESRLNGCLKNEAFYIEENRKLATELESLRLELEASVAKNTEISESKTEHALEASQLKEMLTKSKEEVARYIEENRNLATEIESLRLEFQTSVSKNAEISESKTMHALEASQMKVMLTKSKEEVAHALDEIESRKRSEASQLRRIHELEAEGESVVSDKPDYDRLKAELECAVLSLECCKEEKEKLVSLLQEREDEKSKLADELSLIREQIAQKEENGSVNMQENQGFTERVYEYEGSPLVNDSNQLTVVNDQFRTQHLMSCMEQLDEELEKMKNDNSHLPLTNYDPSFQDLQRELAQLHQANEDLASIYPNFVDFRGNGNALERVLALEIELAEALRAKKKSSIQFQSYLTRNLELYAPPLAIWRTGIEIRHRGLWHIWFSFLKQHSDEEAVFKSFRDINELIKDMLEIKGKYANVENELKEMHDRYSQLSLQFAEVEGERQKLMMTLKSVRSSRNMLRLNRSPVAALGDQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CNS3</accession>
    <name>A0AAD5CNS3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_002250</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007373">
        <property type="protein sequence ID" value="KAI7745049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CNS3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.10:FF:000002">
        <property type="entry name" value="Heat shock 70 kDa protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45639:SF4">
        <property type="entry name" value="HSC70CB, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45639">
        <property type="entry name" value="HSC70CB, ISOFORM G-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), C-terminal subdomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="188"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="202"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="217"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7745049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="255" mass="28819" checksum="26D61BB03057CAEC" modified="2024-05-29" version="1">MVYGAMLSADVQKAVEKEFEMALQDRVMEETKDKKNAVEAYVYDMRNKLHDKLHEFVTDSDREAFIAKLQETEDWLYEDGEDETKGVYIAKLDELKKQGDPIEQRYKEYSEIGSNVDQLINFVNWYKQAAASSDPKYEHIDISEKQKVLNECSEVENWLREKKQAQDSLPKHADPVLLSSDIRKKSETIDRVCRPIMTKPKPAAPETPSSPAPAQAGEQQPSQEAGNGTNPDANSNAENSDSTAAMETEKPEAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CR25</accession>
    <name>A0AAD5CR25_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">LRAT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_016835</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007405">
        <property type="protein sequence ID" value="KAI7744936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CR25"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1720.10">
        <property type="entry name" value="endopeptidase domain like (from Nostoc punctiforme)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007053">
        <property type="entry name" value="LRAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46137">
        <property type="entry name" value="OS05G0310600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46137:SF4">
        <property type="entry name" value="PROTEIN LEAD-SENSITIVE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04970">
        <property type="entry name" value="LRAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51934">
        <property type="entry name" value="LRAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LRAT" evidence="1">
        <location>
            <begin position="20"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7744936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="260" mass="27888" checksum="7F253E21DACCE2C9" modified="2024-05-29" version="1">MGLLSNRLDRASLKPGDHIYSWRTAYIYAHHGIYVGDDKVVHFTRRGQEVGTGTVLDVLLVSSGPRRSLVPCTTCTHTNDTNGVVSSCLNCFLSGGILYRFEYAVTPGVFLAKARGGTCTLALSDPNEIVVHRAKYLLDNGFGCYNVFKNNCEDFAIYCKTGLLVVDQRTMGQSGQAVSIIGGPLAAVLSTPLRLVTTNVYGMVATAVSVYCASRYAADIGMRRDVMKVDAEDLTRRIATGALTVAEPGIPALVASINAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CSI5</accession>
    <name>A0AAD5CSI5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_023286</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007117">
        <property type="protein sequence ID" value="KAI7745820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CSI5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016628">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041694">
        <property type="entry name" value="ADH_N_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045010">
        <property type="entry name" value="MDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43205">
        <property type="entry name" value="PROSTAGLANDIN REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43205:SF7">
        <property type="entry name" value="PROSTAGLANDIN REDUCTASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16884">
        <property type="entry name" value="ADH_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Oxidoreductase N-terminal" evidence="3">
        <location>
            <begin position="137"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="Alcohol dehydrogenase-like C-terminal" evidence="2">
        <location>
            <begin position="228"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7745820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="386" mass="42823" checksum="5ADFD9B92702B6BE" modified="2024-05-29" version="1">MFGPNEMKDGDHINITLHRSRELEHDECDDHYECGIGLVYDEDGKTKEEEDALEYYKSWNHIIGKDLSGFQSNTTGEYILSHSLYKENLIARYGWYGVLLMLVCFYEIYAPKGGEYVFVSAAAGATGQLVKQLQRCRDPIAGLAVAKVQDSGHSSFKKGDLVWGGTEWEEYSIINAPETLFKIEHTDLPLSYYTGILGVPGMTAYAGFYEICTPKKGEYVFVSAASGAVGQLVGQFAKLSGCYVVGSAGTKEKFINIERVRYFPDGIDIYFENVGGKMLDAVLPNMRINGRISACGMISQYNLEQGDAVRNIFNIITQGVLMKGLIVTHYFHLYPKYMEMIVPLIKQGKICYIEDFAEGLGNAPAALVGLFSGKNVGKQVVVVARE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CSR1</accession>
    <name>A0AAD5CSR1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">O-fucosyltransferase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_016242</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycan metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyltransferase GT106 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007018">
        <property type="protein sequence ID" value="KAI7746164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CSR1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006004">
        <property type="term" value="P:fucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11299">
        <property type="entry name" value="O-FucT_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024709">
        <property type="entry name" value="FucosylTrfase_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019378">
        <property type="entry name" value="GDP-Fuc_O-FucTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31741:SF75">
        <property type="entry name" value="O-FUCOSYLTRANSFERASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31741">
        <property type="entry name" value="OS02G0726500 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10250">
        <property type="entry name" value="O-FucT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009360">
        <property type="entry name" value="UCP009360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="11" id="KW-0294">Fucose metabolism</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00030350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7746164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="439" mass="50325" checksum="4C984E7C4E205366" modified="2024-05-29" version="1">MKKRFVIGKIRVEKLKSCNIMNGRSCKMKMWLIRATTMVLLWTCLVQLTALGGGSWGPRVLKGWPSCFTQDPHTASAALDIKLLPSVPVRVLPHKRVYKNNGYLVVSCNGGLNQMRSAICDMVAIARYLNVTLIVPELDKTSFWADPSEFEDIFDVDHFITSLRDEVRILKQLPPRLKKRVELGIVHTMPPVSWSDISYYHNQILPLIQKYKVVHLNRTDARLANNGQPLELQKLRYAYPWWKEKIINSDLKRKDGLCPLTPEETALTLKALDIDRYIQIYIAAGEIYGGQRRMDSLADAYPRLVRKETLLGPADLQYFQNHSSQMAALDYLVSLESDIFVPTYDGNMAKVVEGHRRFLGFKKTILLDRKILVDLIDRYTSGALSWDEFSDAVKETHAQRMGAPTKRLVMFDRPKEEDYFYANPEECLQPSDDEPVSIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CU70</accession>
    <name>A0AAD5CU70_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Elongation factor 1-alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_027474</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10">This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006760">
        <property type="protein sequence ID" value="KAI7747420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CU70"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01883">
        <property type="entry name" value="EF1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03693">
        <property type="entry name" value="EF1_alpha_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03705">
        <property type="entry name" value="EF1_alpha_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000003">
        <property type="entry name" value="Elongation factor 1-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000005">
        <property type="entry name" value="Elongation factor 1-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000255">
        <property type="entry name" value="Elongation factor 1-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00118_A">
        <property type="entry name" value="EF_Tu_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054696">
        <property type="entry name" value="GTP-eEF1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050100">
        <property type="entry name" value="TRAFAC_GTPase_members"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004539">
        <property type="entry name" value="Transl_elong_EF1A_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00483">
        <property type="entry name" value="EF-1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008969">
        <property type="entry name" value="PRK12317.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23115">
        <property type="entry name" value="TRANSLATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22594">
        <property type="entry name" value="GTP-eEF1A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 10" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="9 10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="11">
        <location>
            <begin position="55"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7747420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="488" mass="54160" checksum="5DE3DB6EC586DC67" modified="2024-05-29" version="1">MVERNTLCYKPIATELIYKAVKHPILFCLHPRNVSKFRQRRNPSNKNSCIMGKEKVHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVRQMICCCNKMDATTPKYSKSRYDEIVKEVSSYLKKVGYNPEKIPFVPISGFEGDNMIERSTNLDWYKGPTLLEALDNVSEPKRPSDKPLRLPLQDVYKIGGIGTVPVGRVETGIIKPGMVVTFGPSGLTTEVKSVEMHHEALQEALPGDNVGFNVKNVAVKDLKRGYVASNSKDDPAKGAAGFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFAELQTKIDRRSGKELEKEPKFLKNGDAGIVKMVPTKPMVVETFSEYPPLGRFAVRDMRQTVAVGVIKSVDKKDPTGLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CYC9</accession>
    <name>A0AAD5CYC9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Auxin-induced protein 15A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_025762</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ARG7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006135">
        <property type="protein sequence ID" value="KAI7750528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CYC9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009733">
        <property type="term" value="P:response to auxin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003676">
        <property type="entry name" value="SAUR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31374">
        <property type="entry name" value="AUXIN-INDUCED PROTEIN-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31374:SF436">
        <property type="entry name" value="PROTEIN SMALL AUXIN UP-REGULATED RNA 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02519">
        <property type="entry name" value="Auxin_inducible"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7750528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="86" mass="9956" checksum="412D334D8EE46990" modified="2024-05-29" version="1">MKRCSSLGKKHGYDEDGLPLDVPKGHFPVYVGVNRTRYIVPISFLTHPEFQQLLRHAEEEFGFHHEMGLTIPCEEVVFRSLTSMIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CYK6</accession>
    <name>A0AAD5CYK6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NAD(P)H dehydrogenase (quinone)</fullName>
            <ecNumber evidence="3">1.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_025673</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a quinone + NADH + H(+) = a quinol + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:46160"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.6.5.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a quinone + NADPH + H(+) = a quinol + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:46164"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.6.5.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WrbA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.6.5.2" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010006164">
        <property type="protein sequence ID" value="KAI7750344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CYK6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003955">
        <property type="term" value="F:NAD(P)H dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.360:FF:000001">
        <property type="entry name" value="NAD(P)H dehydrogenase (Quinone) FQR1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010089">
        <property type="entry name" value="Flavoprotein_WrbA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005025">
        <property type="entry name" value="FMN_Rdtase-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01755">
        <property type="entry name" value="flav_wrbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002999">
        <property type="entry name" value="PRK03767.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30546">
        <property type="entry name" value="FLAVODOXIN-RELATED PROTEIN WRBA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30546:SF47">
        <property type="entry name" value="NAD(P)H DEHYDROGENASE (QUINONE)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03358">
        <property type="entry name" value="FMN_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="6">
        <location>
            <begin position="5"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7750344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="203" mass="21737" checksum="E3A35F8F5D66FEF5" modified="2024-05-29" version="1">MATKVYIVYYSMYGHVEKLAEEIKKGAASVEGVEAKLWQVPETLHEDVLGKMSAPPKSDVPVITANELSEADGFIFGFPTRFGMMSAQFKAFFDSTGGLWRTQQLAGKPAGIFYSTGSQGGGQETTALTAITQLVHHGMIFVPIGYTFGAGMFEMEKVKGGSPYGAGTYAGDGSRQPSDLELEQAFHQGKHIATIAKKLKGAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CYN8</accession>
    <name>A0AAD5CYN8_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_013010</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006093">
        <property type="protein sequence ID" value="KAI7750708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CYN8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041910065" evidence="1">
        <location>
            <begin position="24"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7750708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="89" mass="10002" checksum="2E9C8B7DCC6297F5" modified="2024-05-29" version="1" fragment="single">RVYPRSAPFSLSILVCASLLTTSIKIKNKIKKEEEEFLGDIVVEGDGVTPHFGDHCNSMLLADVGDCIPGPDESSHRPNFERRRMRFLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CYZ8</accession>
    <name>A0AAD5CYZ8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Expansin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_003106</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Causes loosening and extension of plant cell walls by disrupting non-covalent bonding between cellulose microfibrils and matrix glucans. No enzymatic activity has been found.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Secreted</location>
            <location evidence="7">Cell wall</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Membrane</location>
            <topology evidence="7">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the expansin family. Expansin A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006065">
        <property type="protein sequence ID" value="KAI7750888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CYZ8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009664">
        <property type="term" value="P:plant-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22274">
        <property type="entry name" value="DPBB_EXPA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.10:FF:000001">
        <property type="entry name" value="Expansin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.760:FF:000001">
        <property type="entry name" value="Expansin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.760">
        <property type="entry name" value="Expansin, cellulose-binding-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="entry name" value="RlpA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007118">
        <property type="entry name" value="Expan_Lol_pI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002963">
        <property type="entry name" value="Expansin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007112">
        <property type="entry name" value="Expansin/allergen_DPBB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007117">
        <property type="entry name" value="Expansin_CBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036749">
        <property type="entry name" value="Expansin_CBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009009">
        <property type="entry name" value="RlpA-like_DPBB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31867">
        <property type="entry name" value="EXPANSIN-A15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03330">
        <property type="entry name" value="DPBB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01357">
        <property type="entry name" value="Expansin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01226">
        <property type="entry name" value="EXPANSIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01225">
        <property type="entry name" value="EXPANSNFAMLY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00837">
        <property type="entry name" value="DPBB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="Barwin-like endoglucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49590">
        <property type="entry name" value="PHL pollen allergen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50843">
        <property type="entry name" value="EXPANSIN_CBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50842">
        <property type="entry name" value="EXPANSIN_EG45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0134">Cell wall</keyword>
    <keyword evidence="6 7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041773920" description="Expansin" evidence="7">
        <location>
            <begin position="22"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="Expansin-like EG45" evidence="8">
        <location>
            <begin position="39"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="Expansin-like CBD" evidence="9">
        <location>
            <begin position="161"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7750888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="243" mass="26089" checksum="BD2D2F008B44853F" modified="2024-05-29" version="1">MAFSHIIISALLVGFFNLVYGDYGHATFYGGGDASGTMGGACGYGNLYSQGYGTNTAALSTALFNNGLSCGSCYELRCNDDPKWCLPGSIRVTATNFCPPNPAQPNNDGGWCNPPLQHFDLAEPAFLQIAQYRAGIVPVMFQRVPCVKKGGVRFTINGHSYFNLVLITNVGGAGDVHAVSIKGSKTGWQPMSRNWGQNWQSNSYLNGQSLSFQVTTSDGRTITSYNVAPSGWQFGQTFEGGQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D0D0</accession>
    <name>A0AAD5D0D0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Peptidase metallopeptidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_018766</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="11">Can bind about 5 Ca(2+) ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="11">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M10A family. Matrix metalloproteinases (MMPs) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005951">
        <property type="protein sequence ID" value="KAI7751199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D0D0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030574">
        <property type="term" value="P:collagen catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04278">
        <property type="entry name" value="ZnMc_MMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000018">
        <property type="entry name" value="Metalloendoproteinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033739">
        <property type="entry name" value="M10A_MMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001818">
        <property type="entry name" value="Pept_M10_metallopeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021190">
        <property type="entry name" value="Pept_M10A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006026">
        <property type="entry name" value="Peptidase_Metallo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002477">
        <property type="entry name" value="Peptidoglycan-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036365">
        <property type="entry name" value="PGBD-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10201">
        <property type="entry name" value="MATRIX METALLOPROTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10201:SF321">
        <property type="entry name" value="METALLOENDOPROTEINASE 4-MMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00413">
        <property type="entry name" value="Peptidase_M10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01471">
        <property type="entry name" value="PG_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00138">
        <property type="entry name" value="MATRIXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00235">
        <property type="entry name" value="ZnMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47090">
        <property type="entry name" value="PGBD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0732">Signal</keyword>
    <keyword evidence="6 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042066367" description="Peptidase metallopeptidase domain-containing protein" evidence="12">
        <location>
            <begin position="26"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase metallopeptidase" evidence="13">
        <location>
            <begin position="135"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="258"/>
        </location>
    </feature>
    <feature type="binding site" description="in inhibited form" evidence="11">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="219"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="261"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="267"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR621190-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR621190-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7751199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="341" mass="38078" checksum="4476C5464EA19969" modified="2024-05-29" version="1">MFQLLFSYNTFFFLSLLPHLFPARTVPGNFINHSPTATWHNFVGLIDAGKGQNFSGVSDLKKYFHRFGYLRNDVVGNFTDEFDESFESAVVNYQKNFGLTVTGKLDAGTVTQIMSPRCGVSDAVHVSKKYAYFSGEPRWGRSAHDTLTYAFSGDHMIDYMKISDVREAFRRSFSRWSAVIPVNFSESDDYSSADIKIGFYDGDHGDEEPFDGVLGVLAHAFSPENGRLHLDIAETWAIDFKLSESKVAVDLESVATHEIGHVLGLAHSLVKGAVMYPSLSPRTKKVDLKVDDIEGIQALYGSNLDYHYCSLIESDVSSGWFIGINPLNWLTYLVMVVGVLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D1C6</accession>
    <name>A0AAD5D1C6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">M8C21_008070</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">AA19_3_7</strain>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010005730">
        <property type="protein sequence ID" value="KAI7752198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D1C6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000225">
        <property type="entry name" value="calcium-dependent protein kinase 28-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0519">Myristate</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="1"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="16">
        <location>
            <begin position="268"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="17">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAI7752198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="295" mass="33627" checksum="F1314BFFB5AA9A26" modified="2024-05-29" version="1" fragment="single">MILPVAVEDVKREVKILQALSGHENVVQFYNAFEDDSYVYIAMELCEGGELLDRILSKKDSRYTEKDAAIVVRQMLKVAAECHLHGLVHRDMKPENFLFKSPKVDSHLKATDFGLSDFIRPGKKFTDIVGSAYYVAPEVLRRKSGPESDVWSIGVITYILLCGRRPFWDKTEDGIFKEVLRNKPDFRRKPWPSISASAKDFVRKLLVKDPRARLTAAQALSHPWVREGGNASEIPLDISVLSNMRKFVRYSRLKQFALRALASTLDEEEIADLKDQFHAIDIDKSGAISLEEMRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D296</accession>
    <name>A0AAD5D296_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA-directed RNA polymerase III subunit RPC4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_018240</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005824">
        <property type="protein sequence ID" value="KAI7751755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D296"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007811">
        <property type="entry name" value="RPC4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13408">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13408:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05132">
        <property type="entry name" value="RNA_pol_Rpc4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="64"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="133"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="182"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="26"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="72"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="86"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="143"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7751755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="285" mass="31614" checksum="E891B4D9270C478C" modified="2024-05-29" version="1">MVMDDKIAVTAPKPKRKIKFMPKAPPKRDQKLVLPKPEKVEDTEEDDKKEQELLKRFNEALKKPKFKPEVKPTTQTRVSSFSFGPSGAKSGGDAAAASGMGKKEYKEPWDYYKYYPVTLPVRRPYSGNPELLDEQEFKEDSETNAYTESSSKAAEELGLMEEDLEKKLIFIQLPTTLPLPKKPVQTEGVGSSKPLKDTEPPLTGCNLNELPAGLMGKMLVYKSGAVKLKLGDHLYNVSGGLDCSFAQDVVAVHTEEKSCCNIGELNKRAIITPDIDSILDHIKGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D2A3</accession>
    <name>A0AAD5D2A3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Reticulon-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_016980</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Endoplasmic reticulum membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005657">
        <property type="protein sequence ID" value="KAI7752568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D2A3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009617">
        <property type="term" value="P:response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003388">
        <property type="entry name" value="Reticulon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045064">
        <property type="entry name" value="Reticulon-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10994">
        <property type="entry name" value="RETICULON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10994:SF193">
        <property type="entry name" value="RETICULON-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02453">
        <property type="entry name" value="Reticulon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50845">
        <property type="entry name" value="RETICULON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="96"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="117"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="181"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="Reticulon" evidence="8">
        <location>
            <begin position="85"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="15"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="36"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7752568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="262" mass="29784" checksum="4347A25F23B72B82" modified="2024-05-29" version="1">MAEHVNEIETTDESLVDKITDKIHRHDSSSSSSDSESEKKTKYDDDDDVKKHHDAPSTDSSLKPKSNRLFGREKPVHQVFGGGKPADVFLWRNKKISAGVLGGATALWVLFELLEYHLVTLVCHVMILVFAVLFLWSPPRIPEVRIPEDTVLQFASALRNEINHFLQMLRDIASGRDLKKFLAVVAGLWILSIVGSWCNFLTLFYTSFVVLHTVPVLYEKYEDEVDAFAEKAMIELKKQYAVFNDKVVSKIPRGPLKQKKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D527</accession>
    <name>A0AAD5D527_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Histone-lysine N-methyltransferase ASHR1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_031973</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005410">
        <property type="protein sequence ID" value="KAI7753509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D527"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050869">
        <property type="entry name" value="H3K4_H4K5_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002893">
        <property type="entry name" value="Znf_MYND"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12197:SF251">
        <property type="entry name" value="EG:BACR7C10.4 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12197">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SMYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01753">
        <property type="entry name" value="zf-MYND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01360">
        <property type="entry name" value="ZF_MYND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50865">
        <property type="entry name" value="ZF_MYND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SET" evidence="5">
        <location>
            <begin position="12"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="MYND-type" evidence="6">
        <location>
            <begin position="57"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7753509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="484" mass="55031" checksum="3F666BFA0CD98C50" modified="2024-05-29" version="1">MEDLQRLLQHDRALSVSTLPDKGRCLFTTRDFSPGEIILSEEPYVSVPNKSSGESRCDWCFTSSNIKRCSSCHVVWYCGSRCQKSDWKLHRLECQLLSQLEKNRIRSLTPSIRLMVKLYLKKKLQDEKVIPTTVMDNYSLVKNLVSQVGENQLVLYAQMASLVNIILQWPDLNIKETAENFSKLACNAHTICDSELIPLGTGLYPVVSIINHSCYPNSVLVFEGRMATVRAMQHIPKGSEGQDVDIRESAVLEGYRCKNHTCDGFLLRDPESKGFICQQCGLVRDIEEILKIAGEEKTMSEKASAALSSGHTNESLDMYLMVEKLQVKLCHSGSIKLMRTREIILKILMEVQDWKKALTYCRLTIPVYERVYPTTHPLRGLQYYTCGKLNWEGGMWLQWQHEDNIRERDGCVVSVATVLAVIFAMLSREWLLGQTEEAIKSITQAVDVLRITHGTTTPFMKELLGKLEEARAEASYKLSSMDDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D577</accession>
    <name>A0AAD5D577_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_016819</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005560">
        <property type="protein sequence ID" value="KAI7753064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D577"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7753064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="55" mass="6078" checksum="AD44B56426CE136C" modified="2024-05-29" version="1">MPMINQNHLPVVGVVSNHRCYCSVSASKPAVLESSSTSKEENCVWSPANKIYTPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D6M7</accession>
    <name>A0AAD5D6M7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_024823</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010003901">
        <property type="protein sequence ID" value="KAI7754307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D6M7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7754307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="69" mass="7675" checksum="1CB7B6D9598801BB" modified="2024-05-29" version="1">MDATSSGPTTKRAKKGAARATQEEPVIIEEEDPTRISGAGLLEFEFLGEARQRYDEMRQTPLHVARTID</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D6Q0</accession>
    <name>A0AAD5D6Q0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_033433</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010001179">
        <property type="protein sequence ID" value="KAI7755393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D6Q0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7755393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="50" mass="5272" checksum="C7E2955E1E22A551" modified="2024-05-29" version="1">MGLVIGGDVDRGKPPPPALAILFGESVTGLAVECAVEEQRMLEGLSKYEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D7T2</accession>
    <name>A0AAD5D7T2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_004819</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010002608">
        <property type="protein sequence ID" value="KAI7754740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D7T2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029005">
        <property type="entry name" value="LIM-bd/SEUSS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10378">
        <property type="entry name" value="LIM DOMAIN-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="167"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="171"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7754740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="287" mass="29887" checksum="CC305468D9C071A3" modified="2024-05-29" version="1">MKDLMDFCRDTKSGPIDGLKNFPRQTNASKSQMEQISSIPGIPADRNGLSKLVSLSPGVNSALVNSSQQMGNRGAPLSGSAQAALALSNYQNMLVRKNTLNSNSQQEASSSFNIPNQNGYPIQGSSNMAVGGFPQRPMFQVQQNHQQSPSNSGGQAMQQHMIQQLLQDMSNGGGGGGGGGPKPSVSGQSAASGSMGRDGLRYGISPSGTPRPPAVPTRSNSFKGASRSDSSAGGGAGNIGVDNQKAATADLPHLCDDIVQDIASEFTGNCFFENDELEEGMGFDWKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D883</accession>
    <name>A0AAD5D883_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_029098</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005009">
        <property type="protein sequence ID" value="KAI7753966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D883"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7753966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="104" mass="11570" checksum="6F78D1A4E3D6A49C" modified="2024-05-29" version="1" fragment="single">HGLGHDSTVIQPIIDTPTQYISTTAELCFITKCSANDPLFLLQARRGLRTLDDAHADIHKVDGNAWRSTMSKAYDAINDEDSDIVYHIDYQGVKTHPSPTPKHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D8J4</accession>
    <name>A0AAD5D8J4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mediator complex subunit 15 KIX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_011636</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000771">
        <property type="protein sequence ID" value="KAI7755838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D8J4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.20">
        <property type="entry name" value="Coactivator CBP, KIX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036529">
        <property type="entry name" value="KIX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036546">
        <property type="entry name" value="MED15_KIX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044661">
        <property type="entry name" value="MED15a/b/c-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33137">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 15A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33137:SF4">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 15A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16987">
        <property type="entry name" value="KIX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mediator complex subunit 15 KIX" evidence="4">
        <location>
            <begin position="25"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="139"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="217"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="323"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="647"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="139"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="217"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="253"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="323"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="335"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="376"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="647"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="658"/>
            <end position="671"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7755838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="971" mass="108178" checksum="7AF45FCB000E7BFB" modified="2024-05-29" version="1">MDTSNWKPNQGGSSGGGVDSSVEGVDWRTLLAPDTRQKLVIRIMENLKKHPKFTGQEKLHELKDIALRFEEKNFNAATSHGVQQINNQGQTPPIPVPSNHPQAGKQILPQSIHNNLSSSGVSPGSGIACSTSQSSSLQNISIGNNMGNGIPPSSFVSQRQIPGSQNTQQYLYQKQPHQIAKQKLQQNNSNNNTTLTQPLIQQQHQHQNLFQSTHFQPSQLQPTMLQPSSLSTVKQNQQPHLQQSGQPMSQHNQPLVLRQQRQPQQPRVFGQQNNLSNVQSIVQRNNFSAIHRQHLGPQISQKQLQSLGTQSGTSTMLNNQHSSHLLQSTQGQRSQMELQQQVIPQLQTQSAQTQHQLNMKQRHPTSYSQQRAMPEASSTSSDSTAQTRNPNSGDWQEEVYQKVKTMKEKYFQDLVELYRKFIGRLQQHDSLPRQPNGDQVEKLKKYKHLLECFMKFLELPKNKLSLNHKEKLGAYEREIFNLISSQKGKPGSTLQQQRMNSLHQSQQTQMQFTQMQPNENQMNSQMQSVNLQHNSLATLHHNSISNAQQTMMGMLQQPNSNMLPHQQLKPLQSRQMQHQLMHKQPLLHHHQFHPETKHSAMLNVNQVSQPHQINDGNEVKIKQETEMKSESLKLLKLDQQNMLSSVTKSSTPLQPANSPFVVSSPSTPSTSNLPGDVNKISLTVSLLANDGNICTPGVSASPLLAECTNLDVSHANDASVVSGKSTTIEQPLERLLKVVKSISPKSLSSSVRDIGSVVSMVDSIAGSAPGNGSRTAVGSFNDSFNLLKYFENSELESTATSTIKRPRVETKHPLLEEIREINQGLIDTVVDISQENADPYVASEVGKGIIVKCSYSAVALCPNLKLQYASVQMSPIQPLRLLVPANYPNSSPIPLDEVPVEVSKEYEDLSIKAKWRFSSCVRRLSEPMSLEMMARTWDVCARAVILECAQQSGGGTFTSKYGAWEDCLTAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D908</accession>
    <name>A0AAD5D908_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">WPP domain-interacting tail-anchored protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_006044</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010003865">
        <property type="protein sequence ID" value="KAI7754356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D908"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039976">
        <property type="entry name" value="WIT1/WIT2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35705">
        <property type="entry name" value="WPP DOMAIN-INTERACTING TAIL-ANCHORED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35705:SF2">
        <property type="entry name" value="WPP DOMAIN-INTERACTING TAIL-ANCHORED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="377"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="340"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="359"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7754356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="407" mass="46450" checksum="1F5C59470C150EB4" modified="2024-05-29" version="1">MEVKPRLQTVNKVNVLRMLEKSLSRELDLEKKLTESKQDEEDLRLKLRLTEQVAFIMEEAAEVVWGRFLEAENAAVVLMGVSRDVIAQHKLVQFNLNGFNERENGFRLKLENCIKLLNSKDASIEKLNSRITQLVADNLEVSVLREKVKSLEEKVNESESNLKKANESNELFQDRVREMESVIESLKDDVYTAENRAETAEGKMSLLEKQSRDLEIQAQHAKASSEAGQEQQNMLYSAIWDMETLIDELKQKVSVAEMKAENAEEQCLLLTDTNSELTKEVEFLRAEVESLETSLSNANLEKMASTKDISAKTKLIMDTVLQLAKERERITRQDEEIQVLSDASIHDPEPESSVTDSDTNLDDAEMEEPESRSKRMLLFRAIFVLSILVLAAFFINWKPDTFDDNAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D990</accession>
    <name>A0AAD5D990_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">CP-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_028787</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000254">
        <property type="protein sequence ID" value="KAI7756811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D990"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01857">
        <property type="entry name" value="HSR1_MMR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001151">
        <property type="entry name" value="Large subunit GTPase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030378">
        <property type="entry name" value="G_CP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043358">
        <property type="entry name" value="GNL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45709:SF2">
        <property type="entry name" value="LARGE SUBUNIT GTPASE 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45709">
        <property type="entry name" value="LARGE SUBUNIT GTPASE 1 HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51721">
        <property type="entry name" value="G_CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CP-type G" evidence="7">
        <location>
            <begin position="158"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="84"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="434"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="87"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="437"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="469"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="506"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="543"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7756811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="543" mass="60682" checksum="88CEA003212929FF" modified="2024-05-29" version="1" fragment="single">MGKKDKSGLGRALVKHQNHKIQESKEKGQLYYKQHKKVLESVTDVADIDAVIQQADEAVRLFSVDNPAAVNTLIDLESGSSISELTPQERRENQKKEEALHASSLSVPRRPAWNANMTVEELDANEKRAFLVWRRSLARLEENDSLVLTPFEKNLDIWRQLWRVLERSDLLVMVVDARDPLFYRCPDLEAYAREIDEHKRTLLLINKADLLPYSVRLKWAEYFQLHGILYLFWSAKAATAELEGKKHVSSSQETVDDDTKIYGREELLSRLQSEAEEIVQMRSDLHQNSTDGKTGHVTVGFVGYPNVGKSSTINALVGAKRTGVTSTPGKTKHFQTLIITEKLTLCDCPGLVFPSFTSSRYEMIASGVLPIHRMTEHREAVQVVADHVPRYVIEGIYNIKLPKPKPYEPENRPPFAAELLRSYCASRGYVASSGLPDETKAARGSDESESDESDSGDGMTEDAGGSGPRLDDVMDDLKSFDMANGLGPGKVANGARKKVASGASYKQHKKPQRKKDRAWRVKDDGSDGMSVVRVYQKSANVGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D9Q9</accession>
    <name>A0AAD5D9Q9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Serine aminopeptidase S33 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_021559</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000188">
        <property type="protein sequence ID" value="KAI7757051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D9Q9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277">
        <property type="entry name" value="ALPHA/BETA HYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277:SF181">
        <property type="entry name" value="SERINE AMINOPEPTIDASE, S33, ALPHA_BETA HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine aminopeptidase S33" evidence="2">
        <location>
            <begin position="72"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="269"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="278"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7757051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="442" mass="49357" checksum="8EE97878311B3723" modified="2024-05-29" version="1" fragment="single">MGAMTSTVAAKFAFFPPNPPSYKLVVEDDSSGRLKMTDVSDRQNVDVLKLKTKRGSEIVAAYVKNPVASLTLLYSHGNAADLGQMFDLFCELSLHLRVNLLGYDYTGYGQSSGKPSEQNTYADIEAAYRCLIETYGVKEEDVILYGQSVGSGPTLDLASRLSRLRAVVLHSPILSGLRVMYPVKRTYWFDIYKNIDKIPLVRCPVLVIHGTADDVVDCSHGKQLWELCVEKYEPLWIKGGNHCDLELYPEYIRHLKKFISAVEKSGRLKNMTGSSTDNDSKPRISTDCREKSRPSTDVPERSRPSTSTEKSRTSVDKRERAKKSSENVEKSTNAVDQPEKARNSIDRFGGMMRAAMYLCDAEQSVVIIGFNNVDCFKRKLVVVDTLEAYLETICNGLNRAKELGDYGNNVNISEIVSINLLMYKGIEILAFGVEIFVSLVFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DA14</accession>
    <name>A0AAD5DA14_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein trichome birefringence-like 38</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_016358</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PC-esterase family. TBL subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000705">
        <property type="protein sequence ID" value="KAI7755912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DA14"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016413">
        <property type="term" value="F:O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029962">
        <property type="entry name" value="TBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026057">
        <property type="entry name" value="TBL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025846">
        <property type="entry name" value="TBL_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32285:SF42">
        <property type="entry name" value="PROTEIN TRICHOME BIREFRINGENCE-LIKE 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32285">
        <property type="entry name" value="PROTEIN TRICHOME BIREFRINGENCE-LIKE 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13839">
        <property type="entry name" value="PC-Esterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14416">
        <property type="entry name" value="PMR5N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Trichome birefringence-like N-terminal" evidence="8">
        <location>
            <begin position="56"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="Trichome birefringence-like C-terminal" evidence="7">
        <location>
            <begin position="109"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF14416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7755912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="372" mass="41983" checksum="7CF44815DB4FCECE" modified="2024-05-29" version="1">MDFGYQRRCLLLAVVIFLLQVQLFLVVTLAKTQVPLSSSYNITGRSSSLRGRELLGCDLFKGKWVLDNSSPIYAASSCPYIDSEFDCMKHGRSDTQYLKYSWQPDSCNLPRFNGLDLLSKWKGKKVMFVGDSLSENMFQSLACLLHASSPKATFQHKDSLTTLTFQDYGVRVYLYHSTYLVDIVQERIGRVLKLDSIQAGDAWKGMDVLIFNSWHWWTHTGRLQPWDYVQYGSTVSKDMDRLEAYYKGMTTWGKWVDLNVDPSNTKVYFQGISPSHYKGKEWGSSSKNCYGELQPIEGSTYPAGLPDAQGVLKKVLGAVKKPVYLLDITTLSQLRKDAHPSTYGGGSGVDCSHWCVPGLPDTWNQLLYASLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DA63</accession>
    <name>A0AAD5DA63_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_023767</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000147">
        <property type="protein sequence ID" value="KAI7757246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DA63"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000815">
        <property type="term" value="C:ESCRT III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005771">
        <property type="term" value="C:multivesicular body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032511">
        <property type="term" value="P:late endosome to vacuole transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006900">
        <property type="term" value="P:vesicle budding from membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1060">
        <property type="entry name" value="ESAT-6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005024">
        <property type="entry name" value="Snf7_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22761">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22761:SF56">
        <property type="entry name" value="OS11G0123500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03357">
        <property type="entry name" value="Snf7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7757246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="156" mass="17605" checksum="038CD9E059792D29" modified="2024-05-29" version="1">MFSRMFGKPKPEATTVTTLDKLNETLEMLEKKEKVLLKKAAAEVEKAKEFTRAKNKRAAIQCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGANAMKAMQKAVNIDDVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DDZ8</accession>
    <name>A0AAD5DDZ8_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_018715</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000036">
        <property type="protein sequence ID" value="KAI7757994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DDZ8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36806">
        <property type="entry name" value="ADENINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041915667" evidence="1">
        <location>
            <begin position="29"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7757994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="219" mass="23989" checksum="432C7E9931E27806" modified="2024-05-29" version="1">MAIGTTKSPSRLLPVFLLITILPSTSSSLPIPTPSTFHSLFSLSHSLLTRVANLRAARGDIPGSTRAKTLADNIKKHSSGFGFYGVVWSVGWDYLKNYAWRDVKTASFEMMGVVSDVNELVKGFGELMSVDSDVGRVEWVKRNYGTLFKVSKSLVNRLLKVFSQPGPLRDAVEMVRTEIVDGGLLQDCLELGSNDLKGVIQILKDLTSQYASSSSKTEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G1H8</accession>
    <name>A0AAD5G1H8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">M8C21_003599</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">AA19_3_7</strain>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010012096">
        <property type="protein sequence ID" value="KAI7725020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G1H8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009882">
        <property type="term" value="F:blue light photoreceptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05574">
        <property type="entry name" value="STKc_phototropin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000133">
        <property type="entry name" value="LOV domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000002">
        <property type="entry name" value="LOV domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000024">
        <property type="entry name" value="Probable serine/threonine-protein kinase cot-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001610">
        <property type="entry name" value="PAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45637">
        <property type="entry name" value="FLIPPASE KINASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13426">
        <property type="entry name" value="PAS_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00086">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0157">Chromophore</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-0600">Photoreceptor protein</keyword>
    <keyword evidence="13" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PAS" evidence="18">
        <location>
            <begin position="7"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="19">
        <location>
            <begin position="77"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="154"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAI7725020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="526" mass="59488" checksum="C69551830B8BEFBF" modified="2024-05-29" version="1">MRKGIDLATTLERIEKNFVITDPRLPDNPIIFASDSFLELTEYSREEILGKNCRFLQGPETDPSTVKKIRDAIDNQTEVTVQLINYTKSGKRFWNLFHLQPMRDQKGEVQYFIGVQLDGSQHVEPLHNCIPETTANDGAILITESGEELGLKHFRPVKPLGSGDTGSVHLVELNGTGELFAMKAMDKSVMINRNKVHRACAEREILDVLDHPFLPALYASFQTPTHVCLITDYCPGGELFMLLDRQPMKVLKEDAVRFYAAEVLVALEYLHCQGIIYRDLKPENVLIQSTGHVALTDFDLSCLTCCSPQLLVSETSDKKKKRKNKKGQQPPIFMAEPVRASNSFVGTEEYIAPEIISGVGHSSAVDWWAFGILLYEMFYGYTPFRGKTRQRTFANVLHKDLKFPSSISETIDSSSSYGKRIVTIIAADFEAFNFDSRIALNLDSEFDNPERESMIFGSKFYTIGYAQFYELDRESYANGMRMYPIPVRTIGFANPPKLETPVFGADEDEKFKEVDLGLQDLVNNVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G6C5</accession>
    <name>A0AAD5G6C5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Protein trichome birefringence-like 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_021469</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PC-esterase family. TBL subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010726">
        <property type="protein sequence ID" value="KAI7730585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G6C5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016413">
        <property type="term" value="F:O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029962">
        <property type="entry name" value="TBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026057">
        <property type="entry name" value="TBL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025846">
        <property type="entry name" value="TBL_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32285:SF7">
        <property type="entry name" value="PROTEIN TRICHOME BIREFRINGENCE-LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32285">
        <property type="entry name" value="PROTEIN TRICHOME BIREFRINGENCE-LIKE 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13839">
        <property type="entry name" value="PC-Esterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14416">
        <property type="entry name" value="PMR5N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="9"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="domain" description="Trichome birefringence-like N-terminal" evidence="9">
        <location>
            <begin position="77"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="Trichome birefringence-like C-terminal" evidence="8">
        <location>
            <begin position="131"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF14416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7730585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="420" mass="48331" checksum="2BA21F9F7ED0F991" modified="2024-05-29" version="1">MGVFAKPQFSVGVSVVIFSLAFAVLLYTERISLLSSTTLFSCTKRGRSSIKGDRNATEEDSRLKTIVDDRFIFDPDECDVTKGKWVFNPSFQPLYTDLSCPYLDRQVSCVKNGKNDTTYRQWEWHLDDCILPRFDARLALEKIRGKRVMFVGDSLERGQWQSFVCLVEFLIPKHQKSMKRGKVHSVFKIKDHNATIEFYWAPFLVESNTDLKIIADPKKRLLKVDSIEKHAKHWVGVDVLLFNTYVWWMTGTKLRSLWGSFANGEDGYEELEAPVSYRLALRTWANWIDSNVDQNKTRVFFTTMSPTHQRPADWNNDAGIKCYNETEPVKKKGHWGTGSDKRMMSVVRSVMGKMKTPVTLLNITQLSEHRVEAHSSIYTELGGKLLTDEQKADPFKFADCIHWCLPGVPDTWNQIFLASL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G6W6</accession>
    <name>A0AAD5G6W6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_024485</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010500">
        <property type="protein sequence ID" value="KAI7731230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G6W6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47944">
        <property type="entry name" value="CYTOCHROME P450 98A9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47944:SF10">
        <property type="entry name" value="CYTOCHROME P450 98A9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0349">Heme</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="155"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7731230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="179" mass="20768" checksum="3EDA6F892B8D90A0" modified="2024-05-29" version="1">MTLLLLAIISFTLVFLLYTFHQRLRFKLPPGPRPWPIVGNLYHVKPVRFRCYAEWAQQYGPIISVWFGTALNVVVSNSELAKEVLKEKDQQLADRHRIRQEGQGFVWADYGPHYVKVRKVCTLELFTPKRLEALRPIREDEVTAMVESIYNDCTHPDSTTPVPPPQTEHHHSLCLKSHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G9L2</accession>
    <name>A0AAD5G9L2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cysteine synthase</fullName>
            <ecNumber evidence="10">2.5.1.47</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_028590</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate</text>
            <dbReference type="Rhea" id="RHEA:14829"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:58340"/>
            <dbReference type="EC" id="2.5.1.47"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8 10">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the cysteine synthase/cystathionine beta-synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.47" evidence="10"/>
    <dbReference type="EMBL" id="JAMZMK010009933">
        <property type="protein sequence ID" value="KAI7733647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G9L2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004124">
        <property type="term" value="F:cysteine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006535">
        <property type="term" value="P:cysteine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01561">
        <property type="entry name" value="CBS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000006">
        <property type="entry name" value="Cysteine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000130">
        <property type="entry name" value="Cysteine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005856">
        <property type="entry name" value="Cys_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050214">
        <property type="entry name" value="Cys_Synth/Cystath_Beta-Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005859">
        <property type="entry name" value="CysK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001216">
        <property type="entry name" value="P-phosphate_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01139">
        <property type="entry name" value="cysK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01136">
        <property type="entry name" value="cysKM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10314">
        <property type="entry name" value="CYSTATHIONINE BETA-SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00901">
        <property type="entry name" value="CYS_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7 10" id="KW-0198">Cysteine biosynthesis</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8 10" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="48"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="12">
        <location>
            <begin position="112"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="283"/>
            <end position="287"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="371"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="9">
        <location>
            <position position="148"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR605856-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR605856-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7733647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="426" mass="46713" checksum="1EB8EB02665934C4" modified="2024-05-29" version="1" fragment="single">YNLKIKPTINSFSFLFNLHSISLSHSLYYCSSITCNSYFLSLNNNRPIILILILIIILMAALAKLLLKRRNQLICTENLIGAKLYTSQPSFAQRIRDLPKTLPGTNIKKDASQIIGKTPLVYLNNVTEGCGAYVAVKQEMFQPTSSIKDRPALSMINDAEEKKLITPGKTVLIEPTSGNMGISMAFMAALKGYKMVLTMPSYTSLERRVTMRAFGADLILTDPTKGMGGTVKKAYDLLEKTPDAFMLQQFSNPANTKVHFETTGPEIWDDTNGKVDIFVMGIGSGGTVSGVGQYLKSKNPDVKIYGLEPEESNVLNGGKPGPHQITGNGVGFKPDILDMDIMEEVLMVSSEDAVNMARELALKEGLMVGISSGANTVAALRLAKRPENRGKLIVTVHASFGERYLSSVLFEKLRKEAEEMQPVSVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G9Q0</accession>
    <name>A0AAD5G9Q0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein jagunal homolog 1-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_026162</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the jagunal family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009948">
        <property type="protein sequence ID" value="KAI7733539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G9Q0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007029">
        <property type="term" value="P:endoplasmic reticulum organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009787">
        <property type="entry name" value="Jagunal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20955">
        <property type="entry name" value="PROTEIN JAGUNAL HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20955:SF1">
        <property type="entry name" value="PROTEIN JAGUNAL HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07086">
        <property type="entry name" value="Jagunal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="42"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="68"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="99"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="164"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7733539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="199" mass="21622" checksum="6DF6704935F55459" modified="2024-05-29" version="1">MQQRNSGNRRPTGTDGSDFSYRMVVDNRYTKVAKGKSTLSKVLFIQAVLVLVGVLDLVLTYLKKEPVETLAVVSSSITVISILVGELGRKRSRVNFLRFYMAASSIGILGSIASIIQSNIQLKASNSPLLINSNLNSFSLLGQINNPSSMVIHDPSKWETEKAALLKIACVSIGLFVQIFSITTTTSLISNMSPPKRAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GBV9</accession>
    <name>A0AAD5GBV9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pyridine nucleotide-disulphide oxidoreductase dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_032130</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009661">
        <property type="protein sequence ID" value="KAI7734711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GBV9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004362">
        <property type="term" value="F:glutathione-disulfide reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046952">
        <property type="entry name" value="GSHR/TRXR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42737">
        <property type="entry name" value="GLUTATHIONE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42737:SF2">
        <property type="entry name" value="GLUTATHIONE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="FAD/NAD-linked reductases, dimerisation (C-terminal) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyridine nucleotide-disulphide oxidoreductase dimerisation" evidence="6">
        <location>
            <begin position="37"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7734711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="108" mass="11800" checksum="95D6254F66757E4A" modified="2024-05-29" version="1" fragment="single">KTVFGSEPSKLATSLVPCHYVKFIILKLPNKILFSISAYHLSVVGLGEEQAIEQANGDILVYTSTFNPINNSISGRQEKTLMKLIVSTETNKVIGASTCWPDAAEIMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GC74</accession>
    <name>A0AAD5GC74_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Early nodulin-93-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_027333</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009595">
        <property type="protein sequence ID" value="KAI7734996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GC74"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005050">
        <property type="entry name" value="Enod93"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33605">
        <property type="entry name" value="EARLY NODULIN-93"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33605:SF2">
        <property type="entry name" value="EARLY NODULIN-93"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03386">
        <property type="entry name" value="ENOD93"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7734996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="46" mass="4974" checksum="AE6C1E3E43E052AF" modified="2024-05-29" version="1">MVPWARAHLNPTAQALIVSTVAGATYFIVADKTILKTARKNSFNDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GCK5</accession>
    <name>A0AAD5GCK5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_019965</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009485">
        <property type="protein sequence ID" value="KAI7735446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GCK5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050823">
        <property type="entry name" value="Plant_Ser_Thr_Prot_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45621">
        <property type="entry name" value="OS01G0588500 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7735446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="297" mass="33707" checksum="04DE972114584ADE" modified="2024-05-29" version="1">MGHKEWINEVNFLGVVNHPNLVKLVGYCAEDDERGIQRLLVYELMSNKSLEHHLLGRGKSPLPWMTRLKIAEGAARGLAYLHEEMDFQGPAAGLTHVSTVLFIFSKVVGTVGYAAPEYVQTGRLTSKSDVWSFGVVLYELITGRRAVERNLPRNEQKLLEWVKPYISDSKKFHLIIDPRLEGDYPIKSVHRLASLANKCLTKNPKSRPKMSEVVEMLGNIIDDMLPPQEEPEAQTETEIETQVAEETEDGLGETGPGKEEGCGVNYVRRVFEFKELVSLRNRSCGRLDWRYWTHGSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GD39</accession>
    <name>A0AAD5GD39_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Auxin response factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_024755</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Auxin response factors (ARFs) are transcriptional factors that bind specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs).</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimers and heterodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the ARF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009130">
        <property type="protein sequence ID" value="KAI7737064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GD39"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009734">
        <property type="term" value="P:auxin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10017">
        <property type="entry name" value="B3_DNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.1040:FF:000001">
        <property type="entry name" value="Auxin response factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.330.10:FF:000001">
        <property type="entry name" value="Auxin response factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000047">
        <property type="entry name" value="Auxin response factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.330.10">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010525">
        <property type="entry name" value="ARF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044835">
        <property type="entry name" value="ARF_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033389">
        <property type="entry name" value="AUX/IAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003340">
        <property type="entry name" value="B3_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015300">
        <property type="entry name" value="DNA-bd_pseudobarrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053793">
        <property type="entry name" value="PB1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31384">
        <property type="entry name" value="AUXIN RESPONSE FACTOR 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31384:SF10">
        <property type="entry name" value="AUXIN RESPONSE FACTOR 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06507">
        <property type="entry name" value="ARF_AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02309">
        <property type="entry name" value="AUX_IAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02362">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01019">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101936">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50863">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51745">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0927">Auxin signaling pathway</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TF-B3" evidence="11">
        <location>
            <begin position="131"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="PB1" evidence="12">
        <location>
            <begin position="719"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="485"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="681"/>
            <end position="714"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7737064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="842" mass="93100" checksum="E5EEC60639FF8877" modified="2024-05-29" version="1">MSIIQEKLNSSGVNSGAHSLVEEMKLLKEMQDHSVIKKPINSELWHACAGPLVTLPQVAVSTNRTATSQVPNYPNLPSQLLCQVLNATLHADKDTDEIYAQMSLRPVNSEKDVLPIPDFGMKPSRHPNEFFCKTLTPSDTSTHGGFSVPRRAAEKLFPQLDFSMQPPTQELIVRDLHDNTWTFRHIYRGQPKRHLLTTGWSMFVGAKRLKAGDSVLFIRDEKSQLLLGVRRANRQNTSLPSSVLSADSMHIGVLAAAAHAAANRTPFTIFYNPRACPSEFVIPLARYRKAVYGTQLSVGMRFGMMFETDESGKRRYMGTIVGISDVDPIRWPGSKWHNLQVEWDEPGCGDKQSRVSPWDIEAPESLFIFPSLTSSLKRPFNSAFLGAQPEWDMVSRQFMRAPDTINASFSNPSMSNLWPEQLMKMLMKPQIANNSTTTIGTNIQETFPTNRNQLQVENRPQFQLIHTNTNTNTNMTTTTTNTSTATTAAMSNTPPQSETAKPDSKPINSPPSLAHELPFLNQLSPFDSSILHGQQFDSPQIDSSSLNGLFPYPDTNVLNSYPNLGQETWDPQANNNSKCFVQSNTCPSNAYGFKDLSDESQSNPQSGNAYNCLNFEGSNAGSTVVDPSVSSTVLDDFCNLKDIEFQNPSSYLVSNNFSSSQDVQSQITSVSLVDSQAYSMQELPDNSGGASSSNGEFDDTALLNNNNPWQQVAPPPRVRTYTKIQKAGSVGRSIDVSSFKNYDELCSEIEKMFGLEGLLNDSRGSGWKLVYVDFENDVLLVGDDPWEEFVGCVRCIRILSPSEVQQMGEEGMQLLNSNAALQAEMNGGSVSDNGPRTWVGPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GDE9</accession>
    <name>A0AAD5GDE9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">3-hydroxyacyl-[acyl-carrier-protein] dehydratase</fullName>
            <ecNumber evidence="2">4.2.1.59</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_004875</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in unsaturated fatty acids biosynthesis. Catalyzes the dehydration of short chain beta-hydroxyacyl-ACPs and long chain saturated and unsaturated beta-hydroxyacyl-ACPs.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.59" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010009027">
        <property type="protein sequence ID" value="KAI7737424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GDE9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019171">
        <property type="term" value="F:(3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009245">
        <property type="term" value="P:lipid A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01288">
        <property type="entry name" value="FabZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.129.10:FF:000001">
        <property type="entry name" value="3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00406">
        <property type="entry name" value="FabZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013114">
        <property type="entry name" value="FabA_FabZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010084">
        <property type="entry name" value="FabZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01750">
        <property type="entry name" value="fabZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000582">
        <property type="entry name" value="PRK00006.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30272">
        <property type="entry name" value="3-HYDROXYACYL-[ACYL-CARRIER-PROTEIN] DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30272:SF1">
        <property type="entry name" value="3-HYDROXYACYL-[ACYL-CARRIER-PROTEIN] DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07977">
        <property type="entry name" value="FabA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0441">Lipid A biosynthesis</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="47"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="81"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="83"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7737424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="258" mass="28474" checksum="669803B431FDA990" modified="2024-05-29" version="1">MGINLINHQLHHHLHSHCQHHTVLTQVAPSSSMASNAFSHSLLPQFTSPSSSPFSNRTSLRPISRSPSLSTPSITHQTLFITNSSSPSSDNNNNITSKDQTPIELRFPAFPTVLDINQIREILPHRFPFLLVDRVIEYNPGVSAVGIKNVTINDNFFPGHFPERPIMPGVLMVEAMAQVGGLVMLQPEVGGSRDNFFFAGIDKVRFRKPVIAGDTLVMRMTLIKLQKRFGIAKMEGKAYVGGEVVCEGEFLMAMGNSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GF84</accession>
    <name>A0AAD5GF84_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UspA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_007559</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008860">
        <property type="protein sequence ID" value="KAI7738176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GF84"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23659">
        <property type="entry name" value="USP_At3g01520-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044162">
        <property type="entry name" value="PHOS32/34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31966">
        <property type="entry name" value="OS01G0783500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31966:SF3">
        <property type="entry name" value="OS05G0501700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="2">
        <location>
            <begin position="34"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="205"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="230"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7738176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="246" mass="26819" checksum="9205D306C19F1F3E" modified="2024-05-29" version="1">MTSPEKQPPPPAAIVVQPASPRFPKPNPLTTGYYRKVAIAVDLSDESAYAVNWAVQNYLRPGDDVVLLHVRPTSVLYGADWGAVEPPPDEQSQQKLEDDFDTFTTTKANDLAKPLVDNNINFKIHIVKDHDMKERLCLEVERLGLSAVIMGSRGFGASRRRSVKGQRLGSVSDYCVHHCVCPVVVVRFPDDKDGDAAAAAAAVGSPLRVNGSGSESSLHPVPEEDVPEPEFFHDASDKHTDVEKPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GGN7</accession>
    <name>A0AAD5GGN7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Serine protease</fullName>
            <ecNumber evidence="7">3.4.21.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_019638</name>
        <name evidence="11" type="ORF">M8C21_019639</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the peptidase S1B family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S1C family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.-" evidence="7"/>
    <dbReference type="EMBL" id="JAMZMK010008578">
        <property type="protein sequence ID" value="KAI7739690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAMZMK010008578">
        <property type="protein sequence ID" value="KAI7739691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GGN7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000012">
        <property type="entry name" value="protease Do-like 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000131">
        <property type="entry name" value="Protease Do-like 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041517">
        <property type="entry name" value="DEGP_PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046449">
        <property type="entry name" value="DEGP_PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008256">
        <property type="entry name" value="Peptidase_S1B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45980">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45980:SF14">
        <property type="entry name" value="PEPTIDASE DO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17815">
        <property type="entry name" value="PDZ_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13365">
        <property type="entry name" value="Trypsin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00839">
        <property type="entry name" value="V8PROTEASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 7" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0720">Serine protease</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Protease Do-like PDZ" evidence="9">
        <location>
            <begin position="384"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF17815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7739690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7739691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="532" mass="58839" checksum="5ED7CEBF59DD5481" modified="2024-05-29" version="1">MDKPTRKRGRKPKHTPDPNPTPTPTPTTSTSSKRRRKTTKQTDNDGVIVTSPERRASRTIEQNGEEYDVAAVVGKVVPSMDAVVKVFCVHTEPNFSLPWQRKRQYSSSSSGFIIGGRRVLTNAHSVEHYTQVKVKKRGSDVKYLATVLAIGTECDIAMLTVSDDEFWEGVSPLEFGDLPALQDAVTVVGYPIGGDTISVTSGVVSRIEILSYIDAAINSGNSGGPAFNDKGECVGIAFQSLKHEDAENIGYVIPTPVITHFIRDYEKNGDYTGFPILGVEWQKMENPDLRMSMGMGNEHKGVRIKRIEPTAPESNVLSPSDIILSFDGVNVANDGTVPFRHGERIGFSYLVSQKYTGDKALVKVLRNSKIHEFNIKLATHKRLIPAHTGGKPPSYYIIAGFVFTAVSVPYLRSEYGKDYDFDAPVKLLDKHLHAMAETVDEQLVVVSQVLVADINIGYEEIVNTQVIAFNNKPVKNLKSLADMVESCTEEFLKFDLEYEQIVVLRTKMAKKATRDILLTHCIPSAMSDDLKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GIS6</accession>
    <name>A0AAD5GIS6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_009354</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007921">
        <property type="protein sequence ID" value="KAI7742734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GIS6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025322">
        <property type="entry name" value="PADRE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33413">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33413:SF1">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14009">
        <property type="entry name" value="PADRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="117"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="124"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7742734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="162" mass="17828" checksum="8A3CEF6037D050FA" modified="2024-05-29" version="1">MGNCQAAEAATVVLVHPGYKVERIYWSVSARDVMNSNPGHYVAQVLTSMAVKTQNGQPVKQLKLLQPDHTLLIGHVYRLISYEDVLKEFAAKKCVKLGKLLMERGVIEMKKQHTTVDTVSMPVRNGNSSKNGSSSNSNRKRNLSHGGGQWKPALKSISELGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GJV0</accession>
    <name>A0AAD5GJV0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_033784</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ARG7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007209">
        <property type="protein sequence ID" value="KAI7745525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GJV0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009733">
        <property type="term" value="P:response to auxin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003676">
        <property type="entry name" value="SAUR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31929">
        <property type="entry name" value="SAUR-LIKE AUXIN-RESPONSIVE PROTEIN FAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02519">
        <property type="entry name" value="Auxin_inducible"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7745525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="98" mass="11150" checksum="6C69947091F703C4" modified="2024-05-29" version="1">MGIIRFPSLSSKTKNFSKLQGLCKRNKLDVPKGYLAVYVGEIQKTRFVVPLSFLEHPLFQDLLRQSEEEFGFDHPMGGLTICCQEDVFTDLISRLPVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GLD3</accession>
    <name>A0AAD5GLD3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF4042 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_023787</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007514">
        <property type="protein sequence ID" value="KAI7744521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GLD3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025283">
        <property type="entry name" value="DUF4042"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052107">
        <property type="entry name" value="HEAT6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13366:SF0">
        <property type="entry name" value="HEAT REPEAT-CONTAINING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13366">
        <property type="entry name" value="MALARIA ANTIGEN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13251">
        <property type="entry name" value="DUF4042"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4042" evidence="1">
        <location>
            <begin position="348"/>
            <end position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7744521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="1130" mass="123954" checksum="98C7E5F84571DBC4" modified="2024-05-29" version="1">MEASSSTSSSAIRPWRTAFLTLRDEAHNSPPISTLIHLLNSLIFSKSHTLIPAIPNLPIHEVTSDALFLVELAGNISSCRGNDDDALHALVQLSQLRDNVAEFEATKQCLETVRSLVDFNQRTIMLSDNVQLLEFLLKTVSFSQNVIAPCFSGNIQGYSLESRKIGLQRNTLWEIQTTTCTMISEVFSRAGTSLPAHHWKSTVDAFRNVMDALASKGLLVENNVMDRFYTSLLHCLHLVLINAKGPIQDHVAGFVAALRMFLGYGLASKSQIVYKKDSRTTNLNSHSSESKSGAYVPPHVRKKNQMKDFNSLSTSDCPRGTLDYMSSDSDLSDSDASAKGINNFRSSKVRVAAIVCIQDLCRSDPKSFAAQWTILLPSSDVLQARRYEANLMTCLLFDPYLKARIASASTLATMLEGPASVFLQVAEYKESTKLGSFTALSSSLGQILMQLHTGVLHLIKHEKNNGFLTSLFKILTLLISCTPYSRMPDELLPTVISSLHQRIIEGFLTHSDQSGLLAVALNCLTDALSVSPSSLKVNEMFVAEISKGLQDDIASSGLLSTLLHYSKSFSNQAVTLEALQVLKAVAHNYPNVMVLCWKQFSSIICDFVDPPGSSRTGTVNSRLTIGTIGEKVLTSAIKLLDECLRAISGFKGTEDLSDDKLPISPFTHDYIKVKTISSAPSYVLESQTISSDDDSVCPSGSQQWCEAIDKQLSSTLFHSSAMVRAASVTCFAGITSSVFFSLPEDKQDRIITYSISAAFDDEVPSVKSAACRAIGVIACFPQALKSVKILGKFINAAEVNCHHSLVPVRIAASWALANLCDSLRHCIDRFTLARSSVDLMECSKMVSLLIDCSLRLTRDGDKIKANAVRALGNLSRFVPCSSHDVNGTSATSFLQLTWEDSSWLEKMVQAFLSCVTTGNVKVRWNVCHALSNLFLNETLKLQDADWAPSVFSILLLLLRDSSNFKIRIQAAAALATPPTILDYGKSYSDVVQGVLHTLENLGSDQMFAPSSFKYRVALEKQLTSTMLHVLGLASGCLDHPVLDFLTKKASFLEDWLKALCSSLVNQFEAQNDSALNQKHETNQSEAGHDSTLNQKKEVINRAIKSLVTVYEGRNNQAMARRFDKLANEIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GLM5</accession>
    <name>A0AAD5GLM5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_009351</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the AB hydrolase superfamily. Epoxide hydrolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006737">
        <property type="protein sequence ID" value="KAI7747552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GLM5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000639">
        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43329">
        <property type="entry name" value="EPOXIDE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="3">
        <location>
            <begin position="3"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7747552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="244" mass="27517" checksum="D13F06283D06DD6D" modified="2024-05-29" version="1" fragment="single">ASFADLVNDTASILDSLAISKAFVIAKDFGGWIAYALALLHPEKVAGIITANVLFMPPGAFTEHIVLPEGFYVSRWQQPGRAEADFGRFDVKTVVRNIYIMFCQSEMPIAGETEEIMDLVKPLTPLPPWMTEDDIATYGTLYEKNGFQTALQVPYRTMHTVGSEVEDPKIEVPTLVIMGEKDYGMKVPGMEEYVRSGVMKKYVPNSETVYVPNGCHFLHEQFPHHVNQLILHFLNRNRRHLTLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GLY6</accession>
    <name>A0AAD5GLY6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">TLDc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_016579</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007534">
        <property type="protein sequence ID" value="KAI7744448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GLY6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006571">
        <property type="entry name" value="TLDc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354">
        <property type="entry name" value="NUCLEOLAR PROTEIN 7/ESTROGEN RECEPTOR COACTIVATOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354:SF74">
        <property type="entry name" value="TLD-DOMAIN CONTAINING NUCLEOLAR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07534">
        <property type="entry name" value="TLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00584">
        <property type="entry name" value="TLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51886">
        <property type="entry name" value="TLDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="domain" description="TLDc" evidence="3">
        <location>
            <begin position="201"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="133"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="142"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7744448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="370" mass="40544" checksum="614E0036F8CA7A99" modified="2024-05-29" version="1">MAFDICGLLSLFTDETQFFPESSPPTFSPPSSLLLGFLIIIIIIPNAFMERQNKLTNLFSDSPSSSPPHGSPSPLPHKARSHTKGGGYFSSVLTYMRSSNEASPNKHDNNLKPIQSLPTRWKSKGESWQHIPLDTYDEDDEFNPREKSETRSSIREVSSAKEENGDQGSTGSTSGSSEVFEDATEPNTPMRAVIDITIDSAFISPELYVFFQASLPNIVKGCRWVLLYSTLKHGISLRTLIRKSSDLSGPCLLITGDTQGAVFGGLLSGPLTPTPKRKYQGTHETFVFTTLYGAPRLFRPTGANRYFYMCLNDLLALGGGGNFALRLDGDLLTGTSGRCDTFGNQCLAHDESFALKNVEVILLLLSLILL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GM95</accession>
    <name>A0AAD5GM95_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF4378 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_005134</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007444">
        <property type="protein sequence ID" value="KAI7744778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GM95"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051513">
        <property type="term" value="P:regulation of monopolar cell growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025486">
        <property type="entry name" value="DUF4378"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033334">
        <property type="entry name" value="LNG1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31680:SF16">
        <property type="entry name" value="DUF4378 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31680">
        <property type="entry name" value="LONGIFOLIA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14309">
        <property type="entry name" value="DUF4378"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4378" evidence="2">
        <location>
            <begin position="584"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="200"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="265"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="359"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="55"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="214"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="272"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="408"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="433"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="443"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="455"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="764"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7744778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="764" mass="85619" checksum="0279398FC4D455A2" modified="2024-05-29" version="1" fragment="single">LFDRRYLLGQRRHGHHQKKLPQGQSDNGKEFTNASEKPKEKNPNNIVKEKHRVSVESSRNSFSSSCSSLDCSKRVQTESSSFCPSIVSEPSSPTSNKKPDLWVPPADIRDVVKDSMTRKPRVVSVKTVAKDGRKGPAMTHVDSPRPLQQQEQAKPAPYERKDQNLAKLHGISTLTTRGVKEVQHTSRYSYDGRESPYKLKSISSIKDRPRLSLDSKQSSVRNRTNAVDQSQPDEPGSNKRPPSSVVARLMGLEVLKCEALKSKPCLDDEPNSVSRSSRKSGSPRVSNALTRIPLEPAPWKQEAKQPVNKKAQSVYGQIEKRLAEVEFKTSGKDLRALKQILEAIQRTKMSLETAVPNKSVTKPDNAAAESMEITNLPKVKKVNPGRGQARNLKATETKATGGTKKPVSPSSVARNSGTVSPRLQKAKIKKQSNGKVAVSNSKAAREQSRRDSSKTRNLSDFPSESDVSVALQNEPENKFADRLMGYKPMAEQPSPVSVLDAFYTEDTPSPVKKKSYAFKEDEELCFDERDWSQVGIDNLASSTESNQFSNIKFENIKQLVHQIELVDITNDEATINPHDDTNEDHIYINEIILASDLLNNLDSASTIIHLHPTTTLINPELFNILEKTKECTSKSKKIKRKMIFDTINNILAQKLAMSASSEQWTSKRTGEILNGEKLLEELCLEIYNLQTNKASDLYDEVINIINEDVNKKSDDWDHNCNEIPALVLDIERLIFKDLISEIVNAEVSVSQEQHGRHRRQLFVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GMW0</accession>
    <name>A0AAD5GMW0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_019575</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006455">
        <property type="protein sequence ID" value="KAI7748812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GMW0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009873">
        <property type="term" value="P:ethylene-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00018">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.730.10:FF:000001">
        <property type="entry name" value="Ethylene-responsive transcription factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.730.10">
        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001471">
        <property type="entry name" value="AP2/ERF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044808">
        <property type="entry name" value="ERF_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31190">
        <property type="entry name" value="DNA-BINDING DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31190:SF499">
        <property type="entry name" value="ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR ERF105"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00847">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00367">
        <property type="entry name" value="ETHRSPELEMNT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00380">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51032">
        <property type="entry name" value="AP2_ERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0936">Ethylene signaling pathway</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0611">Plant defense</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="9">
        <location>
            <begin position="87"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7748812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="225" mass="25646" checksum="F47960240383B171" modified="2024-05-29" version="1">MSTFDELTALDFIRQHLLSDYESPNNPTNSDSDLSVSDYLTFEDEQNTSSFSVNFTVPEPINKSYKKPYIKIECSEKTDSVLETKVHYRGVRRRPWGKYAAEIRDPKRNGARVWLGTFDTGVEAAKAYDRAAYQMRGSKAILNFPLEVEDLKEKTSKGNKVSVITHGVGCQKRKRDEKEMQVVVKKERLLETVNLPLTPSSCMGAWGENYWGNVMLSPLSQFMVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GNC1</accession>
    <name>A0AAD5GNC1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_014267</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006407">
        <property type="protein sequence ID" value="KAI7749110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GNC1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042752">
        <property type="term" value="P:regulation of circadian rhythm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009409">
        <property type="term" value="P:response to cold"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044678">
        <property type="entry name" value="COR27/28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33676">
        <property type="entry name" value="COLD REGULATED PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33676:SF17">
        <property type="entry name" value="COLD-REGULATED PROTEIN 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7749110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="149" mass="16691" checksum="8A59B797F9D1A85D" modified="2024-05-29" version="1">MNNSRRRDTSTPPVSADLTGVIRQASEINSDSVFGNSAKTAWTNEKHDLYLDHLESSFVKQLHRSILKNHPRGAGKQCFVRAPKVQDNSTTMNLCARDKNSFSNVAGLSTEQTFQGHAYFEEFSDQNFPDEASEQMCVTMSKRSKTIVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GPV4</accession>
    <name>A0AAD5GPV4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_004153</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006720">
        <property type="protein sequence ID" value="KAI7747583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GPV4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15539">
        <property type="entry name" value="PHD1_AIRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017956">
        <property type="entry name" value="AT_hook_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056511">
        <property type="entry name" value="IDM1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042163">
        <property type="entry name" value="PHF12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032308">
        <property type="entry name" value="TDBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46309:SF25">
        <property type="entry name" value="ACYL-COA N-ACYLTRANSFERASE WITH RING_FYVE_PHD-TYPE ZINC FINGER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46309">
        <property type="entry name" value="PHD FINGER PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02178">
        <property type="entry name" value="AT_hook"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23209">
        <property type="entry name" value="IDM1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16135">
        <property type="entry name" value="TDBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00929">
        <property type="entry name" value="ATHOOK"/>
    </dbReference>
    <dbReference type="SMART" id="SM00384">
        <property type="entry name" value="AT_hook"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="8">
        <location>
            <begin position="695"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetyltransferase" evidence="9">
        <location>
            <begin position="844"/>
            <end position="985"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="170"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="216"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="545"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="179"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="227"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="278"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="303"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="549"/>
            <end position="561"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7747583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="1139" mass="127314" checksum="B00E464BECD0460D" modified="2024-05-29" version="1">MECENRSEVVECKSVVEFGGEVKIVSGFENIIPSYMVLNNSLDGSNDVGKEKMEDEKTTVFVQEECEKDVTDGLNVANENHEGVLEIKRCSRGRKKKVMESSDCVKDRSPKKVKDGKDEVEVTGRLLRSRTKTISGSATVDESRLNESVVGFKRKMETECFDHSEFQKEVDKSSQVARRPQKKQKRRGRPPKIQVEAGFGKKIKDECFDQNEIKKEVNERSQLLGRPQKKQKGRGRPPKAQSETGLIKKEDECLDPIDLQKEGNESNQLTGRQQKKIQVKEHKDTVIKKLKGRGRPPKVQAGNLATNTKKPNNNLLKANDVKEGLMIEHVPNQDQESDIAGDNKQVNISEESNDHKTVEETVKGESTAQLNDILSAGERQKKRQLVRDKIANMIMKASWTIEYRPRHGRQYLDAVYVDLKGGTHWSITKAYFSLKKKIETGNADDREISAYFPIPVEEMNVLFKSFSKIRSDKDKKKGKKSKNICKAEIVISGDASSYKILNKKGKGGKTRKGVFKSAVNLANKSSNVKVKKDKFRHEHGVISESAVQRRSKVSQKGRQSRKPCLVARSSNKGADQDDDTCTLYNGKRNILSWMIDSGVIKLGSKLTCKDGRKRNRLLEGQVTSDGIHCSCCNETLDISNFVSHGGGNLDQELKNIYYQSGPSLFTCLLESWNKEVKLTNIRFNHVDVKGDDPNDDTCNICGDGGDLICCDGCPSTFHQSCLDFQNFPSGDWHCIYCCCKFCGLVACGSGASQHAPTSEMLKCCLCEEKFHHSCLQEENVIDVGSSSLSFCGSKCQELHEQLQEYIGVKFELKEGYSWTLLKRYDLSQDLDDPVKVHCNSKLAVAFSVMDECFVPIMDERSGINRIHNVVYNCGSNFRRLDYSGFFTAILEKGGELISAASIRIHGNLLAEMPFIGTRNMYRRQGMCRRLLDAIEAALFSLGVDELVIPAIPELYNTWTNVFGFKPLEESLRQAMKGMSLIVFPGTDMLRKPLLKNQFADKNLSSVAVSGDKAVDCITVENENNTIFNEQTLATHLESGTPTTTLKTSEIHLSPSDDEHKSVGLVDDDVTGVKMSHLTSSAVPKNSFDLNLQPAATDIDIQSFDEKTISTDPQVCKNTFDLSDSIVQVDNTTTQPLVLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GRK0</accession>
    <name>A0AAD5GRK0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Senescence regulator S40</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_000244</name>
        <name evidence="3" type="ORF">M8C21_000248</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the senescence regulator S40 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006361">
        <property type="protein sequence ID" value="KAI7749424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAMZMK010006361">
        <property type="protein sequence ID" value="KAI7749428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GRK0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010150">
        <property type="term" value="P:leaf senescence"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007608">
        <property type="entry name" value="Senescence_reg_S40"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46525">
        <property type="entry name" value="EMB|CAB72159.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46525:SF21">
        <property type="entry name" value="SENESCENCE REGULATOR S40-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04520">
        <property type="entry name" value="Senescence_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00034773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7749424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7749428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="157" mass="17834" checksum="6825CD3230CB7194" modified="2024-05-29" version="1">MAASTTYFGRKNHRYFSVENEIPTSTDLIFEFNEFDVWNDVTSSPEFHKTLSNSRISNKPVPSTANQKAVRGTALSLPVDVPDWSMILKNELIQNHTTGNDEGDDDFDNDDLIPPHEYLARGRTMSFSVHEGIGRTLKGRDLSRVRNAVWKQIGFED</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GRS5</accession>
    <name>A0AAD5GRS5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">G3BP-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_008174</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006197">
        <property type="protein sequence ID" value="KAI7750146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GRS5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00780">
        <property type="entry name" value="NTF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.50:FF:000003">
        <property type="entry name" value="Nuclear transport factor 2 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002075">
        <property type="entry name" value="NTF2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018222">
        <property type="entry name" value="Nuclear_transport_factor_2_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039539">
        <property type="entry name" value="Ras_GTPase_bind_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10693:SF83">
        <property type="entry name" value="NUCLEAR TRANSPORT FACTOR 2, NTF2-LIKE DOMAIN PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10693">
        <property type="entry name" value="RAS GTPASE-ACTIVATING PROTEIN-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02136">
        <property type="entry name" value="NTF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50177">
        <property type="entry name" value="NTF2_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="NTF2" evidence="5">
        <location>
            <begin position="14"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="308"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="171"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="383"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="179"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7750146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="425" mass="47798" checksum="0BD3B167C6E68607" modified="2024-05-29" version="1">MTSLFSSSITASQVGSYFVQQYYEVLQQQPEFAHQFYTGSSTMIRIDGESTETASSIYQIHTLVQSSYFSGIEIKTINSVESWSEGIIVAVSGSVKSNRFHGWRKFVQTFFLAPQEKGYFVINDIFHFVSEDVTNHLPPSLATVHKDDFQPVYSFSKELSGSKDHGDYYASQERHYQQQEEDDSDTEDYEEESPVEQLSFDRVDYFQESFHQNTVDYDQEPFHQNTSSYVQEPIQAVNKPAPEPMKLTYASILQSNGKSMPPNPSQAPVIKSVAPTTKWHQPPEPVASSETTSHVPEDPLINIEGEYMSVYVRNLPTSVTSEEIFQEFKNFGKIKHDGVFIKNRKDVGICFAFVEFEDISGVHKAIEASPIQLAGKQVYIEERRANSSSSSRGGRGRGGRGRGSYDSTRGHNGGVSNGRSNGLRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GRX5</accession>
    <name>A0AAD5GRX5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_018168</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005977">
        <property type="protein sequence ID" value="KAI7751119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GRX5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7751119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="112" mass="12267" checksum="84D7445A7D81ACFB" modified="2024-05-29" version="1" fragment="single">MEFSMVGPYVVDVSVLIGGRNLLPWCKMMVTHCCGSSYYKNIMGGCKSAYACSLLLHQEIHWPYPNSAMHDVVAADNEDSKTLIEILRVAVTLIPDAAIANLSWTAAVGYDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2024-11-27" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GTY5</accession>
    <name>A0AAD5GTY5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_004981</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005540">
        <property type="protein sequence ID" value="KAI7753114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7753114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="274" mass="29638" checksum="ED346BAD2A1843FD" modified="2024-05-29" version="1" fragment="single">VFSYGMVVGLGVCFAKGAGSNLGVLGRCLDTWLSSRVGGAAAISDSPPLRXXXXLIQRLSYRPGYVGGRKIEKRMPARRRWPSQAALGGVCVAAPSLSLIYRSGTLSPIPFCYLRFMGRPEVKGFGGRSEQYSEGCLMGYDGSLACVPGVNGLRQRCCRGDAEVVGRFESACQSTEVYWISTGHLTYDPNVRKGVAKQLSIDHEPSKERDHIEDRGGFVSTFPGDDCLVCGLNSWLGNNILSGSIPDLGTLKMLKAFHDDEVENGKECHFGVND</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GU15</accession>
    <name>A0AAD5GU15_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CUE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_032629</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005703">
        <property type="protein sequence ID" value="KAI7752366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GU15"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48459">
        <property type="entry name" value="CUE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48459:SF1">
        <property type="entry name" value="CUE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="567"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="447"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="567"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="630"/>
            <end position="639"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7752366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="639" mass="69798" checksum="C5C38F629FCA9D3E" modified="2024-05-29" version="1">MGSENVFRVLQELFPKVDSILLQYAALQHPYDADSAAEVVLTKTMDVRPERPLTIGSSSNSHSSLSFSNDGIKKTQAESSMDRVFVNPFNVGSSSNSKSSLGSFNGFTFRADGGKKRPVTVNPFFAGHSNKNHVSSMDGNLLNATKAGSSSNSQSSSSFFDGTTFRTDGVKTPQVASTMDHVTVNPFIANHSTKDHVSSIDGSSKALDAAALSTQFTSHTVGENVSCEPFVMSVDDETNSETEAKCDGVPLPLETFGGSNSITADSESIGTENVDNEALIARERKQLVGTPETNLDATQESDPICSTKTLEKDDLADNFANSEDESAMSSVVTRSEQVCSTEYLDEVIEDAKNKKETFELAMNSVIDLMKAVESKEQDAEQANENATRGCSDILAKVDEVKHALVRAKETNDMHAGEVNAERAILATEMRELQVRLFNLSDERNKSLEILEEMRKALDIRMAAALEEIATAEDKKLERERSAKEALLYQESQMEKVVEESKKLKLVAEENSKLQEFITDRGHVIDILQGEMAVKCQDVLLLKEKFDKRIPLTGSLFASQASSILVSPSSPFRSTTPLEHETETLRTPKSSSLDGSPKSPDKETASFSNGKAKKDDDELKGLLGDDGWELFNNNGDLLFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GUW5</accession>
    <name>A0AAD5GUW5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Pectate lyase</fullName>
            <ecNumber evidence="3 8">4.2.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_029216</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>Eliminative cleavage of (1-&gt;4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.</text>
            <dbReference type="EC" id="4.2.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="8">Binds 1 Ca(2+) ion. Required for its activity.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 2/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the polysaccharide lyase 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.2.2" evidence="3 8"/>
    <dbReference type="EMBL" id="JAMZMK010005635">
        <property type="protein sequence ID" value="KAI7752683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GUW5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030570">
        <property type="term" value="F:pectate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018082">
        <property type="entry name" value="AmbAllergen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002022">
        <property type="entry name" value="Pec_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045032">
        <property type="entry name" value="PEL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31683:SF144">
        <property type="entry name" value="PECTATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31683">
        <property type="entry name" value="PECTATE LYASE 18-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00544">
        <property type="entry name" value="Pectate_lyase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00807">
        <property type="entry name" value="AMBALLERGEN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00656">
        <property type="entry name" value="Amb_all"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0106">Calcium</keyword>
    <keyword evidence="7 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Pectate lyase" evidence="9">
        <location>
            <begin position="47"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7752683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="335" mass="37416" checksum="99CF435D2D294ACE" modified="2024-05-29" version="1">MATMLPYADADSTLRGMAGVAEGFGRFAVGGVNGPLHHVTSLADEGPGSLREACRKKEPLWIVFDISGTIHLSSYLNVSSHKTIDGRGQRIKLTGKGLRLKECENIIICNLEFEGGRGHDVDGIQIKPNSKHIWIDRCSLRDYDDGLIDITRQSTDITISRCYFSGHDKTMLIGADPTHIDDRCIRVTIHHCFFDGTCQRHPRLRFGKVHLYNNYTRNWRVYAICASVEAQIYSQCNIYEAGQKKKAFEFYTEKAADKEEASSGLITSEGDLLLNGAQPCASKEAIEEPMFHPTEFYPKWTMQPASDTLKHTLHTFTGWQSVSRPAEQGNVVASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GV55</accession>
    <name>A0AAD5GV55_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Histone H2A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_010542</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 10">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">Belongs to the histone H2A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005426">
        <property type="protein sequence ID" value="KAI7753476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GV55"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000792">
        <property type="term" value="C:heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070828">
        <property type="term" value="P:heterochromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00074">
        <property type="entry name" value="HFD_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000026">
        <property type="entry name" value="Histone H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002119">
        <property type="entry name" value="Histone_H2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032454">
        <property type="entry name" value="Histone_H2A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032458">
        <property type="entry name" value="Histone_H2A_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23430">
        <property type="entry name" value="HISTONE H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16211">
        <property type="entry name" value="Histone_H2A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00620">
        <property type="entry name" value="HISTONEH2A"/>
    </dbReference>
    <dbReference type="SMART" id="SM00414">
        <property type="entry name" value="H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00046">
        <property type="entry name" value="HISTONE_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 10" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="12">
        <location>
            <begin position="20"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="Histone H2A C-terminal" evidence="13">
        <location>
            <begin position="99"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF16211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7753476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="148" mass="15491" checksum="53D659087BB1A16E" modified="2024-05-29" version="1">MDGAGKAKKGAGGRKGGPRKKAVTRSIKAGLQFPVGRIGRYLKKGRYAKRVGTGAPVYLAAVLEYLAAEVLELAGNAARDNKKNRIIPRHVLLAIRNDEELGKLLAGVTIAHGGVLPNINPVLLPKKTAAKEAKSPAKATKSPKKAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GV89</accession>
    <name>A0AAD5GV89_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_002206</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005397">
        <property type="protein sequence ID" value="KAI7753566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GV89"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05283">
        <property type="entry name" value="CAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000022">
        <property type="entry name" value="Cinnamyl alcohol dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.180.10:FF:000126">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047109">
        <property type="entry name" value="CAD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029752">
        <property type="entry name" value="D-isomer_DH_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42683">
        <property type="entry name" value="ALDEHYDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00065">
        <property type="entry name" value="D_2_HYDROXYACID_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="6">
        <location>
            <begin position="23"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7753566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="294" mass="31534" checksum="38AF5D4550D5B4A7" modified="2024-05-29" version="1">MAKSPELEHPFKAYGYAAQDASGVLAPFNFSRRATGEKDVRFKVLYCGICHSDLHFIKNEWGFTTYPVIPGHEIVGVVTEVGSKVDKFKVGDKVGVGCIVQSCRSCQSCVNDLENYCPKQIITYGVPDLDGTRTYGGYSDHMVTDEHFVLRWPENLPLDAGAPLLCAGITTYSPLRHFGLDKPGVKVGVVGLGGLGHLAVKFAKAFGAEVTVFSTSPAKKQEALEGLKADHFIVSKDLEQMQAAASSLDGIIDTVSATHPIAPLLSLLKPDGKLVLVGAPEKPLELAAFSLIMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GWG0</accession>
    <name>A0AAD5GWG0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">GATA-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_015921</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the type IV zinc-finger family. Class A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010002475">
        <property type="protein sequence ID" value="KAI7754781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GWG0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00202">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000018">
        <property type="entry name" value="GATA transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051140">
        <property type="entry name" value="GATA_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45658">
        <property type="entry name" value="GATA TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45658:SF51">
        <property type="entry name" value="GATA TRANSCRIPTION FACTOR 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00320">
        <property type="entry name" value="GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00401">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00344">
        <property type="entry name" value="GATA_ZN_FINGER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0010">Activator</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GATA-type" evidence="11">
        <location>
            <begin position="333"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="205"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="282"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="208"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7754781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="433" mass="48405" checksum="4FC230328BF08889" modified="2024-05-29" version="1" fragment="single">KNSYHLTHTFTPTIYTHFPPFLSSFKLLLFTSIPPFFISFLKINVFPVPILNHFSFSVQFPPSSLKFSPLQLFQDQSELFYFRKKMAEQNFIEEIDCASFFDHIDDLIDFPPVNDNALVSVDCNEFSSIWANNSDDLQVSDPIFCGGNGDDASNLSAELYVPYEDIVQMEWLSNSVDDSFSGGGLTVKKEDVPVEKETVVGHRQFQTSSPVSVLESSSSSSSSTSYSGSGSGCGKMIPLSPIQCGAQKARTKRPRPATFNPRDMVDLLSPLVLMPVSGSLETGKNFTSCHGPRLVSRDADQKKKKKIKKPKIQEENENRNQNQNQNPTGQGVRKCLHCEITKTPQWRAGPMGPKTLCNACGVRYKSGRLFPEYRPAASPTFVPTLHSNSHKKVVEMRTKFGPNDKMVTVRTEPEPKFEGEEGCYRTELAQLNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-02-05" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GWP3</accession>
    <name>A0AAD5GWP3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Terpene cyclase/mutase family member</fullName>
            <ecNumber evidence="4">5.4.99.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_023612</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the terpene cyclase/mutase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.-" evidence="4"/>
    <dbReference type="EMBL" id="JAMZMK010000768">
        <property type="protein sequence ID" value="KAI7755844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016871">
        <property type="term" value="F:cycloartenol synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016104">
        <property type="term" value="P:triterpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02892">
        <property type="entry name" value="SQCY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.20:FF:000002">
        <property type="entry name" value="Terpene cyclase/mutase family member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.20:FF:000022">
        <property type="entry name" value="Terpene cyclase/mutase family member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032696">
        <property type="entry name" value="SQ_cyclase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032697">
        <property type="entry name" value="SQ_cyclase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018333">
        <property type="entry name" value="Squalene_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002365">
        <property type="entry name" value="Terpene_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01787">
        <property type="entry name" value="squalene_cyclas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11764:SF20">
        <property type="entry name" value="LANOSTEROL SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11764">
        <property type="entry name" value="TERPENE CYCLASE/MUTASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13243">
        <property type="entry name" value="SQHop_cyclase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13249">
        <property type="entry name" value="SQHop_cyclase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01016">
        <property type="entry name" value="Prenyltransferase_Like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01074">
        <property type="entry name" value="TERPENE_SYNTHASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Squalene cyclase N-terminal" evidence="6">
        <location>
            <begin position="141"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="Squalene cyclase C-terminal" evidence="5">
        <location>
            <begin position="394"/>
            <end position="731"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7755844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="739" mass="83806" checksum="7A15EC3131B9D929" modified="2024-05-29" version="1">MWRLKIAEGGSPWLRTTNDHVGRQFWEFDPTLGSVEELAEVEKVRKTFRDNRFEKKHSSDLLMRXXXMRLGNVSYIVFAKERSLSASPPKVNIKDAEDITEDKVTNVLRRAIGFHSTLQSDDGHWPGDYGGPMFLLPGLNIDGGWGLHIEGHSTMFGSALNYVTLRLLGEGANDGDGAMEKGRKWILDHGGATYITSWGKFWLSVLGVFEWSGNNPMPPEMWILPYFLPVHPGRMWCHCRMVYLPMSYIYGRRLVGPITSTVLALRKELFTVPYHDINWNDARNLCAKEDLYYPHPLVQDILWATLDKFVEPVLMRWPGKKLREKALRTAMEHIHYEDENTRYICIGPVNKVLNMLCCWAEDPNSEAFKLHLPRIHDFLWLAEDGMKMQGYNGSQLWDTAFAIQAIIATNLIEDFGPTLKKGHMYIKNTQVLDDCYGDLDYWYRHISKGAWPFSTADHGWPISDCTAEGLKAALLLSTLPSEIVDEPLDAVRLYDAVNVLLSLQNSDGGFATYELTRSYSWLELVNPAETFGDIVIDYPYVECTSAAIQALVSFKKLYPGHRKEEVQRCIDKAAAFIESIQASDGSWYGSWAVCFTYGTWFGVKGLVAAGKSFSNCSSIRKACNFLLSKQLASGGWGESYLSCQNKVYTNLEGNRSHVVNTGWAMLALIDAEQAKRDPKPLHSAARVLINSQLENGDFPQEEIMGVFNRNCMITYAAYRNIFPIWALGEYRCRVLQAQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GWR3</accession>
    <name>A0AAD5GWR3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_013711</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000005">
        <property type="protein sequence ID" value="KAI7758350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GWR3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd14733">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18186">
        <property type="entry name" value="BTB_POZ_ZBTB_KLHL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044714">
        <property type="entry name" value="AtSIBP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46672:SF4">
        <property type="entry name" value="OS08G0495500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46672">
        <property type="entry name" value="OS08G0495500 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="2">
        <location>
            <begin position="163"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7758350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="328" mass="37165" checksum="E5ECF56C4447BA6A" modified="2024-05-29" version="1">MTDNAYRVDTTSRLAQWRIDNLASCTYRKSDPFKIGRWNWRLILEKNRNLLIKLFPEMSSANKESPPIASFIIRVVSSLGGRKALVHPEIRDKQLKSSEDFVWALEVPLTGKFIIELEFLDLKAASPNGGEASSVLSEGFTQKDLSATALSSLGRMLSESIHTDIIINATDGSIGAHRAVLAARSPVFRSMFSHNLKEKEMNVINISDMSIEACQVFLSYIYGNIIDQDFLDHRLDLLRAADKYDVMDLKEACHESLLQDIDTNNVLERLQNASLYHLPKLKICCMQYLVKFGKIYDILDEFNAFIQSADMELVGEVFNEVLSVWKGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BJS3</accession>
    <name>A0AAD5BJS3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transmembrane protein 50 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_016866</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0220 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012225">
        <property type="protein sequence ID" value="KAI7724415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BJS3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007919">
        <property type="entry name" value="UPF0220"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13180">
        <property type="entry name" value="SMALL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05255">
        <property type="entry name" value="UPF0220"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="78"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="110"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7724415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="135" mass="14789" checksum="EF32CB736B6F0A89" modified="2024-05-29" version="1">MDVSEMLAIFGPGLAGAVFGAGWWFWVDAVVCSAVKVSFLHYLPGIFASLAALMFNCVRRDDIDYSPYEEGEWRLKLWLFLAYVVSFVSLAASVGLLIQDTLVPEGPSAWTGTAGVLQCVFVLISGLVYWTSHSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BKL3</accession>
    <name>A0AAD5BKL3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Replication factor A C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_014089</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012141">
        <property type="protein sequence ID" value="KAI7724906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BKL3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013955">
        <property type="entry name" value="Rep_factor-A_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47165">
        <property type="entry name" value="OS03G0429900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47165:SF4">
        <property type="entry name" value="OS03G0429900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08646">
        <property type="entry name" value="Rep_fac-A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Replication factor A C-terminal" evidence="1">
        <location>
            <begin position="77"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7724906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="181" mass="20371" checksum="9E7F3CE1B3B30539" modified="2024-05-29" version="1">MNNKYNCFDTGTLQLKCTNGTYVYVNPTAVETGQLQDLYVSGIPPAPAAIAVAILTTTLSDILQLEKKEAQGKTFVINGHISEVHAYKEWYYKACPACHQCVYEKKSGWYCTRDENLVKPSNMFRLPATITDGTGNIEATVFDKVARYLLNKTCDELLEMSNPVDAFLKIENQETQYHIQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BNB5</accession>
    <name>A0AAD5BNB5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_016216</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIIA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011642">
        <property type="protein sequence ID" value="KAI7726526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BNB5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000211">
        <property type="entry name" value="Plasma membrane ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42861">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00120">
        <property type="entry name" value="HATPASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="202"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="57"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7726526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="249" mass="27820" checksum="7CBAD45236FEE253" modified="2024-05-29" version="1">MSSRTSVCLWKAFTNPRMRRLVRRHDLTNLRPSLLRTRLAGKGSWHEGRSISLSIMQGPQERAPAVAPPAAPASQPAAQPEAWNGNEYVPLFIPHGPIQRCIYCYIPIEELIEKADGFAGVFPEHKYEIVKKLQERKHICGMTGDDVNDAPTLKRVDIGIAVADATDAARGASDIVLTEPGLSVIMSAVLTSRAIFQRMKNCTIYAVSITIRVVLGFMLLALIWKFDFSPFMVLILLFLTMEPSRPYQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-04-02" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BP19</accession>
    <name>A0AAD5BP19_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_025203</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011625">
        <property type="protein sequence ID" value="KAI7726629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48163">
        <property type="entry name" value="BNAC02G25670D PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48163:SF2">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="60"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="322"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="46"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7726629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="387" mass="43573" checksum="C558DCF60651F2A4" modified="2024-05-29" version="1">MASHHASLGRRTLEEIRQKRAAQKLSKTSSGPDLTLPPNPTEVFGIKKSSSGSGISESDISGLVSQLQELQKRNVELDEENRRLSSELHSDKVENDVLRKRVNDLEQNTVPSLRRALKDVAMEKDAAVVAREDFSAQVRALKKRLKEAEEEQYRAEEDAASLRAEXXXXXXXXXXXXXXRGGPPDHMQAIEKELADLKSQLEEDKERLEKQLHDMAVAVERLESSRQKLLLEIDSQSSEIERLFEENSNLSSAYQEATGMVTQWENQVKDCLKQNEELRNMLDKLRTEQASISIINDREAHKRNMESNSGVSEISQAYTAEVVSLKGQLAKEQSKAETLSAEVLQLSAQLQQAVQAYNGLARLYKPVLRNIENNLLKMKQDGSLTVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BQ31</accession>
    <name>A0AAD5BQ31_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Deoxyhypusine hydroxylase</fullName>
            <shortName evidence="9">DOHH</shortName>
            <ecNumber evidence="9">1.14.99.29</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Deoxyhypusine dioxygenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Deoxyhypusine monooxygenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_003835</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the hydroxylation of the N(6)-(4-aminobutyl)-L-lysine intermediate to form hypusine, an essential post-translational modification only found in mature eIF-5A factor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>[eIF5A protein]-deoxyhypusine + AH2 + O2 = [eIF5A protein]-hypusine + A + H2O</text>
            <dbReference type="Rhea" id="RHEA:14101"/>
            <dbReference type="Rhea" id="RHEA-COMP:10144"/>
            <dbReference type="Rhea" id="RHEA-COMP:12592"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:82657"/>
            <dbReference type="ChEBI" id="CHEBI:91175"/>
            <dbReference type="EC" id="1.14.99.29"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="9">Binds 2 Fe(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Protein modification; eIF5A hypusination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the deoxyhypusine hydroxylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.99.29" evidence="9"/>
    <dbReference type="EMBL" id="JAMZMK010011356">
        <property type="protein sequence ID" value="KAI7727531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BQ31"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019135">
        <property type="term" value="F:deoxyhypusine monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000099">
        <property type="entry name" value="Deoxyhypusine hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000292">
        <property type="entry name" value="Deoxyhypusine hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03101">
        <property type="entry name" value="Deoxyhypusine_hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027517">
        <property type="entry name" value="Deoxyhypusine_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004155">
        <property type="entry name" value="PBS_lyase_HEAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12697:SF5">
        <property type="entry name" value="DEOXYHYPUSINE HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12697">
        <property type="entry name" value="PBS LYASE HEAT-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13646">
        <property type="entry name" value="HEAT_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03130">
        <property type="entry name" value="HEAT_PBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00567">
        <property type="entry name" value="EZ_HEAT"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0386">Hypusine biosynthesis</keyword>
    <keyword evidence="6 9" id="KW-0408">Iron</keyword>
    <keyword evidence="3 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="222"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="254"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="255"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7727531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="317" mass="34541" checksum="91CD8C48D19A0F43" modified="2024-05-29" version="1">MAVDGKSFEVNPETEKFLCDRLLDQTQPISERFRVLFSLRNLRGPAPRNALIQATKDPSNLLAHEAAFALGQMQDADAIPALTLVLNDLSLHPIVRHEAAEALGAIGLESNICLLKDSLDSDPAQEVRETCELALSRIQELKNVGESNQLSTTEASPFLSVDPAAPASCSSVNELREAILNEEKGMYERYAALFGLRNHGGDEAVAAIIESLNAKSALLRHEVAYVLGQLQNKAASDALSRVLKDVNEHPMVRHEAAEALGSIADDQCISLLEEFSTDPEPIVSQSCEVALSMLDFERSGKSFEYLFMQAPQLQEAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BQK9</accession>
    <name>A0AAD5BQK9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_021812</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ornithine cyclodeaminase/mu-crystallin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011335">
        <property type="protein sequence ID" value="KAI7727700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BQK9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1780.10">
        <property type="entry name" value="ornithine cyclodeaminase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003462">
        <property type="entry name" value="ODC_Mu_crystall"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023401">
        <property type="entry name" value="ODC_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13812">
        <property type="entry name" value="KETIMINE REDUCTASE MU-CRYSTALLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13812:SF19">
        <property type="entry name" value="KETIMINE REDUCTASE MU-CRYSTALLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02423">
        <property type="entry name" value="OCD_Mu_crystall"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001439">
        <property type="entry name" value="CryM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7727700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="282" mass="30492" checksum="FA6D45C4AF63D8A2" modified="2024-05-29" version="1">MATVTGNLMNPTPIFIDAATIKSVLPYKSLINHLQTSLSSATSVIESPIRHSHPTSSSSTLLLMPAWSLSSTTLPYIGVKLVTTHPNNSSLNLPGVHASYVLFHSLTGQTLASMDGTEITVRRTACVSALASHYLSHLNSQVLLMIGAGSLAPHLIKAHLTTRPSINRVMIWNRTWLKAGAHLDLVGSYKPSMRECDDEAVKRGRVFVDNEAALVEAGELVGAFERGVMKKEEIEGDLVELTKGVKIGRKDEKEITLFVSVGSAVVDLLSAQLAYETIRVKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BQV5</accession>
    <name>A0AAD5BQV5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Protein RETICULATA-RELATED 1, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_021027</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RETICULATA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011531">
        <property type="protein sequence ID" value="KAI7726803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BQV5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009706">
        <property type="term" value="C:chloroplast inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099402">
        <property type="term" value="P:plant organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021825">
        <property type="entry name" value="RETICULATA-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31038">
        <property type="entry name" value="EXPRESSED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31038:SF2">
        <property type="entry name" value="PROTEIN RETICULATA-RELATED 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11891">
        <property type="entry name" value="RETICULATA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0934">Plastid</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="412"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="412"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="429"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7726803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="442" mass="48617" checksum="85C25CA513A74451" modified="2024-05-29" version="1" fragment="single">NPLLPVSGYRGVVRKGLVVRNSSAAVDGDSVAALERCFRMTPVECPAASSVAAHGPVMKGGQYGAFGAVTLEKSKLDMTQKQTKSSPELFDRKFLEAVLNEWQKTMMDLPSGLRQAYEMGLVSSAQLVKYIAMNARPTTARFISRSLPQGLSRGFIGRMIADPAFLYRFLLEQAATIGSSVLWEVKNRKERIKEEWDLALINVLTVTACNAAVVWSLAPCRSYGNTFRFDLQNTLQKLPNNVFEKSYPMREFDMQKRIQSFFYKAAELCMVGLTAGAAQGALTNLVARKKEGRLSVTIPSVSTNALGYGAFLGIYANLRYQLLCGFDRAMVTQFDVIGVGLAFSTALRIMNAQLGETSRLAWLGVEVDPLAHSDDLLKQAYNRPSETANTSPKWFISKNPVTSGLGLLGFKQDQSQTSTEGGSQPPPPKARRKRIVRKKVTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BR37</accession>
    <name>A0AAD5BR37_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_002815</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011282">
        <property type="protein sequence ID" value="KAI7727970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BR37"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7727970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="213" mass="23212" checksum="E561D5F18CCBB16B" modified="2024-05-29" version="1">MEEDNGNAETLTLFQSSLSPQLFEEVSHSLSELDGKAVESSTLIEVAKVLSSQPDFDVQELNNLLIRLNCGKALEPSTSIEVAKELSSEVDFDVQEISDLLIRLNHGKTFEPMTSVEVVKGLDTDLDFDVQEISDLLIGLNCGIPFEPLTMVEVARGLASDLDFDVQQVSDLLIGLNCGDPFEPLTLVEVANGVALELDFDVQELSDLLNGLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-06-18" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BRW7</accession>
    <name>A0AAD5BRW7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Actin-interacting protein 1-2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_001056</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Binds actin. Enhances the F-actin depolymerization activity of actin-depolymerizing factor (ADF) proteins.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011193">
        <property type="protein sequence ID" value="KAI7728538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030864">
        <property type="term" value="C:cortical actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030042">
        <property type="term" value="P:actin filament depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000102">
        <property type="entry name" value="Actin-interacting protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000167">
        <property type="entry name" value="Actin-interacting protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19856:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19856">
        <property type="entry name" value="WD-REPEATCONTAINING PROTEIN WDR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="19"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="150"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="195"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="444"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="487"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00058835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7728538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="628" mass="68658" checksum="A315E7F8D8D5DD93" modified="2024-05-29" version="1" fragment="single">NGRSVIIRYLDRPLEVQVYAEHAYQTTVARFSPNGEWVASADVSGMVRIWGTHNGFVLKNEFRVLSGRIDDLQWSGDGMRIVASGDGKGKSFVRAFMWDSGSNVGEFDGHSRRVLSCAFKPTRPFRIVTCGEDFLINFYEGPPFKFKLSHRDHSNFVNCVRFSPDGSKFITVSSDKQGLLYDGKIAEKKGELSSEDGHKGSIYAVSWSPDSKQVLTVSADKTAKIWTISEDFNGTVAKTLCCPGSGGVEDMLVGCLWQNDYIVTVSLGGTIYLYSASDLDKDPTILCGHMKNITSLVVLKTNPETILSSSYDGLISKWIRGVGYNGKLERKDKNQIKXXXXLLASTIRIPLTGDECGDANIVDIGSQPIDLSVAIHKHELALISIEKGVVLLNGTQVLSTIDLGFTVSACAIAPDGTEAIVGGQDGKLHIYSVNGDSLTEEAVLEKHRGAITVIHYSPDVSMFASADANREAVVWDRVTREVKLKNMLYHTARINSLAWSPDNTMVATGSLDTCVIVYEISKPASSRITIKGANLGGVYAVSFVDDNTVVSSGEDACILAVVDSDAFLGWVDPWSTRPYFVKDFSEKPSMLAQKTSWILKILLVGTSDHWTFKACSRDSDLVVVLLHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BSI2</accession>
    <name>A0AAD5BSI2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">inorganic diphosphatase</fullName>
            <ecNumber evidence="3">3.6.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_019091</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>diphosphate + H2O = 2 phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24576"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="EC" id="3.6.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.1" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010011166">
        <property type="protein sequence ID" value="KAI7728720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BSI2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004427">
        <property type="term" value="F:inorganic diphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006796">
        <property type="term" value="P:phosphate-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00412">
        <property type="entry name" value="pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.80.10:FF:000002">
        <property type="entry name" value="Soluble inorganic pyrophosphatase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.80.10">
        <property type="entry name" value="Inorganic pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00209">
        <property type="entry name" value="Inorganic_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008162">
        <property type="entry name" value="Pyrophosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036649">
        <property type="entry name" value="Pyrophosphatase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10286">
        <property type="entry name" value="INORGANIC PYROPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00719">
        <property type="entry name" value="Pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50324">
        <property type="entry name" value="Inorganic pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00387">
        <property type="entry name" value="PPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7728720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="228" mass="26144" checksum="0BEE75A20C582A8B" modified="2024-05-29" version="1">MTPPIETPNKTAVSHHHKHPPLNERILSSMKRRSAASHPWHDLEIGPDAPTIFNCVIEISKGSKVKYELDKKTGLIKVDRVLYSSVVYPHNYGFVPRTLCEDNDPLDVLVIMQACQFILIIHFSLEPILPGCFLRAKAIGVMPMIDQGEKDDKIIAVCADDPEYRHYNDIKELPPHRLAEIRRFFEDYKKNENKEVAVNDFLPSAEAIEVVKHSMDLYADYIVESLRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BTB2</accession>
    <name>A0AAD5BTB2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cyclin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_007322</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the cyclin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011072">
        <property type="protein sequence ID" value="KAI7729090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BTB2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000212">
        <property type="entry name" value="Cyclin-T1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043198">
        <property type="entry name" value="Cyclin/Ssn8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10026">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028758">
        <property type="entry name" value="Cyclin, C/H/G types"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0195">Cyclin</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="4">
        <location>
            <begin position="31"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="4">
        <location>
            <begin position="146"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7729090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="231" mass="26306" checksum="96C8FA77D7E987F4" modified="2024-05-29" version="1">MSRDEIERLSPSRKDGIDALQETHLRYSYCAFLQNLGLRLELPQTTIGTAMVLCHRFFVRRSHASHDRFLVATATLFLAAKSEETPCPLNNVLRASSEIFHKQDFNVLCYLLPADWYDQYRERVLEAEQMVLTTLNFELNVQHPYAHLTSILDKLGLSQSLLVSLALSLVSEGLRSSLWLQFKPHQIAAGAAYLAARSLNMDLTSCQNVWQEFSTSPSVLKDVVQQLMELF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BVC2</accession>
    <name>A0AAD5BVC2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Xrn1 helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_020526</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011060">
        <property type="protein sequence ID" value="KAI7729143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BVC2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004534">
        <property type="term" value="F:5'-3' RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000956">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.1050">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027073">
        <property type="entry name" value="5_3_exoribonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041412">
        <property type="entry name" value="Xrn1_helical"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12341:SF41">
        <property type="entry name" value="5'-3' EXORIBONUCLEASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12341">
        <property type="entry name" value="5'-&gt;3' EXORIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17846">
        <property type="entry name" value="XRN_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Xrn1 helical" evidence="1">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF17846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7729143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="86" mass="9893" checksum="B0D829693173765B" modified="2024-05-29" version="1" fragment="single">MNGKRFAWQGIAKLPFIDEDRLLAEVKKVEHTLTEEEARRNSRMADMLFVASSHTLSPYVFSLNDRCKQLSANERVQVKEQIDPSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BVK1</accession>
    <name>A0AAD5BVK1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rab3-GAP regulatory subunit N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_025944</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010781">
        <property type="protein sequence ID" value="KAI7730442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BVK1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026059">
        <property type="entry name" value="Rab3GAP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032839">
        <property type="entry name" value="RAB3GAP_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12472:SF0">
        <property type="entry name" value="RAB3 GTPASE-ACTIVATING PROTEIN NON-CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12472">
        <property type="entry name" value="RAB3-GAP REGULATORY DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14655">
        <property type="entry name" value="RAB3GAP2_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab3-GAP regulatory subunit N-terminal" evidence="1">
        <location>
            <begin position="28"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="Rab3-GAP regulatory subunit N-terminal" evidence="1">
        <location>
            <begin position="317"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7730442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="416" mass="46491" checksum="89B3DFEAB5AA11D2" modified="2024-05-29" version="1">MARRTHLTDLGTIANLDLTELGAGKEGWLDNPNLLCAFDTDSLAISNRYFLLILEWSHPSAPASFRVKIRPNLSPIEAEYVSALEWLVFDDIRVIAVGTSCGYLLIYSLAGDLIHKQLVYPGKILRLRVRGTKRDITEDTSSSEEVCVVIPGIIARFDGSDIQRVLQRWYQETQNRFWNQSSIDKGSEEHEVSSSPIPYQCWNVNKYGSCVDAAVTGVMPPPLLELQVVFSYLNSAAFIVEQSSDRYYCAVTIGADAAISAFRLSEDRSRSFVGALLSKVVPATFSTIASFSKLVWRTRQPTKKPEPKPQPFARGLGYRDASCLFVERLVNKDSRERVKNDYCLCLAIHAPRKGIVEIWQMRTGPRILTVPCPKGSRMLQPTYRFGSTLAASSSASYKPLEVFFLNGDSGQLSRLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BVT8</accession>
    <name>A0AAD5BVT8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nitrogen regulatory protein areA GATA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_027409</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010784">
        <property type="protein sequence ID" value="KAI7730387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BVT8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013860">
        <property type="entry name" value="AreA_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044161">
        <property type="entry name" value="SPS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46039:SF7">
        <property type="entry name" value="SUCROSE-PHOSPHATE SYNTHASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46039">
        <property type="entry name" value="SUCROSE-PHOSPHATE SYNTHASE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08550">
        <property type="entry name" value="GATA_AreA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nitrogen regulatory protein areA GATA-like" evidence="4">
        <location>
            <begin position="151"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="193"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7730387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="373" mass="41904" checksum="2101564378D10C83" modified="2024-05-29" version="1" fragment="single">ASETAWNAARNKVADESTSLDKSVRINGTTTEEANVCRNHDLISGVCGLARMEAPFPKHEKTVASTAPNEKLPKFYPADDVKKPLFNKRKARPTKLRASITLGTVLNILAGRFKGKRVVFLKQLTSGLLLVTGNTAEMIKSIRDSEERITRLENMSWRIWNLARGKKKAKELNRKGNTKRELSRVPKSLLADMPEELSEGEKRDTVSDISPKGGISTKGGMKRINSVDVLKNFATQNKERKLYIVLISLHGLIRGENMELGRDSDTGGPVKYVDAAILHQSLMKRIGMISVSFVKDAEVIYELKNYLKIPKKRYSQMHKRLKEVQHFLWHQPLASLFVETKKGAKVAFVEAEAIYDDKQYSNGDSSDRSTCKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BW37</accession>
    <name>A0AAD5BW37_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_032305</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010681">
        <property type="protein sequence ID" value="KAI7730696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BW37"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035251">
        <property type="term" value="F:UDP-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016138">
        <property type="term" value="P:glycoside biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000060">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48047:SF28">
        <property type="entry name" value="F11M15.8 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48047">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7730696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="357" mass="38921" checksum="E4C95B2356DB9FDC" modified="2024-05-29" version="1">MQWFSAHINPPVALISDFFLGWTQHLADQLDIPRICFYSSGAFLSAVLDHCCHNMSLVRPQEMTVFSELPNSPTFAWEHLPSLLRVGNESDPEWKVAVDGNIANRSSWGWVVNTFDALEGEYLEYLRKLLGHGRVFGVGPVSLLGGSDPMTRGHSGHGSDFDVVGWLDGKGDGCVVYVCFGSQKFLTNDQLEGLVNGLEDSGVSYVLVVKAEQGSLVRAGSGRGVVIKGWAPQVEILNHLAVGGFLSHCGWNSVLEAILAGVMILAWPMEADQYIDARLLVEDHGIAVRVCEGPNTVPDSARLARIIAESMSGDRVEKLKSKEMKNKAIEAVKGGSSSMDLERLVKELSNFKPKIKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BXN9</accession>
    <name>A0AAD5BXN9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-CH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_000468</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010421">
        <property type="protein sequence ID" value="KAI7731596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BXN9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16495">
        <property type="entry name" value="RING_CH-C4HC3_MARCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000318">
        <property type="entry name" value="E3 ubiquitin-protein ligase MARCH4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022143">
        <property type="entry name" value="DUF3675"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033275">
        <property type="entry name" value="MARCH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23012:SF216">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE MARCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23012">
        <property type="entry name" value="RING/FYVE/PHD ZINC FINGER DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12428">
        <property type="entry name" value="DUF3675"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12906">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51292">
        <property type="entry name" value="ZF_RING_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-CH-type" evidence="4">
        <location>
            <begin position="16"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7731596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="153" mass="17165" checksum="3773A0DDFC86007F" modified="2024-05-29" version="1">MREVGLNINMDEEFIYSASEVICCRICHEAEFESCKPLEAPCSCSGTVKFAHRDCVQRWCDEKGNTNCEICLQKFEPGYLAPPPKKTTQDTHVTIRESVEASRTDDNLVIIAIADEEEESLVEREYAECSSTADATASMCRTLAIVDTERDNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BYZ8</accession>
    <name>A0AAD5BYZ8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Calcineurin B-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_030254</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Acts as a calcium sensor. CBL proteins interact with CIPK serine-threonine protein kinases. Binding of a CBL protein to the regulatory NAF domain of a CIPK protein lead to the activation of the kinase in a calcium-dependent manner.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer. Interacts with CIPK.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the calcineurin regulatory subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010516">
        <property type="protein sequence ID" value="KAI7731183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BYZ8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019900">
        <property type="term" value="F:kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000073">
        <property type="entry name" value="calcineurin B-like protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045198">
        <property type="entry name" value="CNBL1-10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23056">
        <property type="entry name" value="CALCINEURIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23056:SF116">
        <property type="entry name" value="CALCINEURIN B-LIKE PROTEIN 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13202">
        <property type="entry name" value="EF-hand_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13833">
        <property type="entry name" value="EF-hand_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="83"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="120"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="164"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU369080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7731183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="227" mass="25965" checksum="1FD6C8CDF13107AE" modified="2024-05-29" version="1">MSQCLEGIKHLCASLLYCCDFELSKQSQGLDDPAVLASQTVFSVSEIEALYELFKKISSSAVNDDGLINKEKFQLALFKTNKKESLFADRVFDLFDTKHNGILGFEEFARALSVFHPNAPIDDKINFSFQLYDLKQQGFIERQEVKQMVVATLAESGMNLSDEVIESIIDKTFEEADTKHDGKIDQEEWRNLVLRHPSLLKNMTLQYLKDITTTFPSFVFHSRVEDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BZE7</accession>
    <name>A0AAD5BZE7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">UDP-arabinopyranose mutase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_010046</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RGP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010260">
        <property type="protein sequence ID" value="KAI7732307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BZE7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009505">
        <property type="term" value="C:plant-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052691">
        <property type="term" value="F:UDP-arabinopyranose mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071669">
        <property type="term" value="P:plant-type cell wall organization or biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033356">
        <property type="term" value="P:UDP-L-arabinose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037595">
        <property type="entry name" value="RGP_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31682:SF43">
        <property type="entry name" value="UDP-ARABINOPYRANOSE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31682">
        <property type="entry name" value="UDP-ARABINOSE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03214">
        <property type="entry name" value="RGP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="3" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="244"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="247"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7732307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="794" mass="90546" checksum="B2C74CC7CCFE1E1E" modified="2024-05-29" version="1">MKVAKDPSGKEINALAQHINNLLTPAAPFFFNTLYDPYREGADFVRGYPFSLREGVTTAISHGLWLNIPDYDAPTQLVKPLERNTRYVDAVLTIPKGTLFPMCGMNLGFHREIIGPAMYFGLMGDGQPIGRYDDMWAGWCAKVICDHLGLGVKTGLPYIWHSKASNPFVNLKKEYKGIYWQEDIIPFFQNVTLPKECTTPQKCYIELSKLVKEKLGPIDPYFEKLSDAMVTWIDAWDELNPSTDGVTTAKAEPSKSKTTTTTSTTALHRHPSTLNRHIRPQQTHTITTKTTQFITLFITTFKTLIKKRIRDFQIPHVNLALYMIQSNNEIRKMWEWVIIVKLSLHISSSVSKAQILLPLNYISSSSPRRNLHRYGHQRRHTTTPLQPKKTVCRRMETVGAIKIIPRGKMMAVSCMHTHNKYPLLNPLNSPPHRHHVCSINRHQTAFDIVIPTIRNLDFLEQWRPFFQPYHLIIVQDGDPSIKIRVPEGYDYELYNRNDINRILGAKASCISFKDGACRCFGFLVSKKKYIFTIDDDCFVAKDPSGKEINALAQHVHNLSTPSTPFFFNTLYDPYREGADFVRGYPFSLREGVTTAISHGLWLNIPDYDAPTQLVKPRERNTRYVDAVMTIPKGTLFPMCAMNLAFDRELIGPAMYFGLMGQGHPIGRYDDMWAGWCAKVTCDHLGLGVKTGLPYLSHSKASNPFVNLKKEFKGLFWQEELIPFFQNVTLPEECTTAQKCYIELSKLVKEKLVSIDPYFEKLSDAMVTWIDAWNELNPSTDGEATVKVNKSSKPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C0A5</accession>
    <name>A0AAD5C0A5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Serine aminopeptidase S33 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_021625</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010398">
        <property type="protein sequence ID" value="KAI7731734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C0A5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000131">
        <property type="entry name" value="Monoglyceride lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051044">
        <property type="entry name" value="MAG_DAG_Lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11614">
        <property type="entry name" value="PHOSPHOLIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine aminopeptidase S33" evidence="1">
        <location>
            <begin position="130"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7731734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="388" mass="43691" checksum="A80CC3663757FFA4" modified="2024-05-29" version="1">METSIHMEELTSGASNRVLFFSSASLRRALFFLRSIFLWVIFHLRRRQPPHHHHRLPPSSIPKFSFRRIDVKIEEDVLRRRALAHEIRMLTSSIDDGDVLGSQCKAATWMFSGTGRNALFCRSWIPMSNEIRGILIIIHGLNEHSGRYADFARQLNSCNFGVYAMDWIGHGGSDGLHGYVPSLDHVVADTGSFVEKIKSDHPGIPCFLYGHSTGGSVVLKAASYPYIKELVEGVILTSPALRVTPSHPIVGAVAPLFSLVAPKYQFKGANKRGIPVSRDPAALVAKYTDPLVYTGPMRIRTGHEILRISSHLNRNFKSVTVPFFVLHGTADKVTDPLASQELYNHAASNYKHIKLYDGYLHDLLFEPEREEIGQDIIDWMEMKLSFHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C0A8</accession>
    <name>A0AAD5C0A8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein SIEVE ELEMENT OCCLUSION B-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_005140</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010178">
        <property type="protein sequence ID" value="KAI7732707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010088">
        <property type="term" value="P:phloem development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027944">
        <property type="entry name" value="SEO_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027942">
        <property type="entry name" value="SEO_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039299">
        <property type="entry name" value="SEOA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33232">
        <property type="entry name" value="PROTEIN SIEVE ELEMENT OCCLUSION B-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33232:SF20">
        <property type="entry name" value="PROTEIN SIEVE ELEMENT OCCLUSION B-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14577">
        <property type="entry name" value="SEO_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14576">
        <property type="entry name" value="SEO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sieve element occlusion N-terminal" evidence="1">
        <location>
            <begin position="20"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="Sieve element occlusion C-terminal" evidence="2">
        <location>
            <begin position="471"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="domain" description="Sieve element occlusion C-terminal" evidence="2">
        <location>
            <begin position="542"/>
            <end position="670"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7732707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="671" mass="76800" checksum="CDC4BAF5D7018F1C" modified="2024-05-29" version="1">MATSRTRRPRGDRHMFSSADDNAMMKNILATHSPDGTYVDVIPLLQIIEDIMQRAHPDASHHGPQSELDAIVDSVLYNDVNEMLEVMALTINKVACEISCKCIGGGDAHATTTGIFNMLSNYGWDAKAVITLAAFAVNYGEFWLVAQLYIANPLAKSLAHLKQLPAVLEHGDSLKPRFSAVTSLIKAMLDVTHCIIKFKELPEQYISPDTPELVIATTHIPTAVYWIIRSIVSCASILINLIGMGHEHVTTTTEAWELSSLAHKISNIHDHLKHHLDLCNHHINEKKHAEAYLTLLRVMDTPHLDNIKPLKHLIYLKDDQLPLYESSTKNRVSIEVLRKKIVLLLISDLDLPPEELTVLDQMYREARQDPTRPESQYEVVWLPVVPNHMTTPWTEENQIKFEGLRNMMPWYSVFHPSLLDSAVIKYIKEVWHFMKKPMLVVMDPQGRIVNTNALHMLWIWGSIAFPFTSLREEALWREETWRIELLTDSIEPMIFNWVAEGKYICLYXXXXWNGYENLQPQHKPWLVGPESSSKCYMWGKATRGKNMLHSKLQHGKSFDEDPILREINVMLTYDGNDQGWAVICRGSDDWMRRVNGDTILTSLTKYEEWMNEAKDKGFLIALNGYLEANRPPHHCNRLILPGTTGNVPERVVCAECGRSMERFFLYQCCTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C1X9</accession>
    <name>A0AAD5C1X9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_032042</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009879">
        <property type="protein sequence ID" value="KAI7733923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C1X9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043452">
        <property type="entry name" value="BZIP46-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22952:SF463">
        <property type="entry name" value="ABSCISIC ACID-INSENSITIVE 5-LIKE PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22952">
        <property type="entry name" value="CAMP-RESPONSE ELEMENT BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BZIP" evidence="5">
        <location>
            <begin position="349"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS00036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7733923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="399" mass="43595" checksum="360824F9567AD542" modified="2024-05-29" version="1" fragment="single">MVANSPLTRQSSIYSLTFDELQNTLGGGGKDFGSMNMDELLKNIWTAEETQAVASTSSFGIDGNAPNTGNIQRQGSLTLPRTLSRKTVDEVWRELLITSGNGAKDGDLMGQTNLQPQEREPTLGEMTLEDFLLKAGVVKEDHVVQTSSRPVVPQNGSFFGEVSQPNVENSSFIFGFQNPNQNQGFNGPSVNRITEAKTGVTLQPTPTPTPKSQLQPIFPKQAALDFTAPLTGQPKQAALHFTAPLTGQPKQAALDFTAPLTGQLASPRAKLGKFEQPVKTSVVQASDIENGVINIKSLGGISVNSPRNLIPKSNLDSTPSPPFYAFGEGDLRGRKTSGTLEKVVERRRRRMIKNRESAARSRARKQEELMERQNNQVVEKMKLPWGNKRLCLRRTLTGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C269</accession>
    <name>A0AAD5C269_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_023797</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009968">
        <property type="protein sequence ID" value="KAI7733499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C269"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7733499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="50" mass="5837" checksum="A5D06954FB9F184B" modified="2024-05-29" version="1">MSNPDRDPHVFLLVQFAAINNYQGKMSVTNSYEASRVFINEDIEELRTFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C2K6</accession>
    <name>A0AAD5C2K6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">U6 snRNA phosphodiesterase 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">3'-5' RNA exonuclease USB1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_011429</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010103">
        <property type="protein sequence ID" value="KAI7732949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C2K6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034477">
        <property type="term" value="P:U6 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1140.10">
        <property type="entry name" value="Cyclic phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027521">
        <property type="entry name" value="Usb1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13522">
        <property type="entry name" value="U6 SNRNA PHOSPHODIESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13522:SF3">
        <property type="entry name" value="U6 SNRNA PHOSPHODIESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09749">
        <property type="entry name" value="HVSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="7"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00030030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7732949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="164" mass="18266" checksum="0185EAF7FB1D3607" modified="2024-05-29" version="1">MEALIASYGDTSSDSDSESQPSINNQSSPSATSLPPPPIHLLNPPNTLGTFDYLERNSGSRIRSFPHIEGNYALHVYIPVFIPSERKKELAQFLKKTASLVPGLHVVDIDVPLHMLLKDEEKMAQAALGREFHISLGRTVPIRVHQIDSVVAMLRQKLQFQKRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C3R0</accession>
    <name>A0AAD5C3R0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_025070</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009694">
        <property type="protein sequence ID" value="KAI7734542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C3R0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080043">
        <property type="term" value="F:quercetin 3-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080044">
        <property type="term" value="F:quercetin 7-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000040">
        <property type="entry name" value="UDP-glycosyltransferase 76C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000120">
        <property type="entry name" value="UDP-glycosyltransferase 76C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926:SF1561">
        <property type="entry name" value="CYTOKININ 7-BETA-GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926">
        <property type="entry name" value="GLUCOSYL/GLUCURONOSYL TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7734542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="460" mass="51463" checksum="ED244F0654B36B55" modified="2024-05-29" version="1">MDIIATTTTSSRRRRLVLLPYPLQGHINPMLQLANILHSKGFAITILHTHFNAPDPSNNPHFNFMPIPGVAEENLPVSSTDNLIRLMHYLNDSCLQPIQGCLEQLLSEVAGVACLITDAQWFAVQTIADGLKLPRIVNWTSSVSAFLSFAAVPLLRQMGFFRPLSGDEMKSEELILGVEPLRVRDVSRFLTSDPEGASELMELMVQGMKPAQAIIWNTFKELEEFQLPKLSRGFPNQHFLIGPFHKYFPTLSSSLLTQDKSCMSWLDKQVPNSVLYVSFGSLAGVEEFEFLEIAWGLANSKQPFLWVVRPGSVKGSEWLEPLPDGFLETIVKGRGYIVKWAPQEEVLAHRSTGGFWTHNGWNSTLESICEGVPMICSPSFADQIPHARNVSDVWKIGVELKNGFERGEIESAIKTLMVDKKGLETRDRIINLKEKVNLCLKKGGSSYSSLEDLTNYILSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C4S6</accession>
    <name>A0AAD5C4S6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">methylmalonate-semialdehyde dehydrogenase (CoA acylating)</fullName>
            <ecNumber evidence="2">1.2.1.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_008922</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.27" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010009576">
        <property type="protein sequence ID" value="KAI7735045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C4S6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004491">
        <property type="term" value="F:methylmalonate-semialdehyde dehydrogenase (acylating, NAD) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006574">
        <property type="term" value="P:L-valine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006210">
        <property type="term" value="P:thymine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07085">
        <property type="entry name" value="ALDH_F6_MMSDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000002">
        <property type="entry name" value="Methylmalonate-semialdehyde dehydrogenase (Acylating)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000003">
        <property type="entry name" value="Methylmalonate-semialdehyde dehydrogenase [acylating]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010061">
        <property type="entry name" value="MeMal-semiAld_DH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01722">
        <property type="entry name" value="MMSDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43866">
        <property type="entry name" value="MALONATE-SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43866:SF3">
        <property type="entry name" value="METHYLMALONATE-SEMIALDEHYDE DEHYDROGENASE [ACYLATING], MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="5">
        <location>
            <begin position="46"/>
            <end position="505"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7735045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="527" mass="56716" checksum="EC5979C9FC1EB0B7" modified="2024-05-29" version="1">MLRLSIRRATTTFRPQIFVLKYYSNISTDRHSDSPPIVPNLIGGSFVDSQSSHFIDVINPATQQVVSQIPLTKNEEFKAAVSAAKQAFPLWRNTPVTTRQRIMLKLQQLIRRDMDKLAMNITTEQGKTLKDAQGDVFRGLEVVEHACGMASLQMGEFSSNVSHGIDTYSIREPLGVCAGICPFNFPAMIPLWMFPVAVTTGNTFVLKPSEKDPGASMILAELAMEAGLPSGVLNVVHGTNDIVNAICDDEDIKAISFVGSNTAGMHIYARASANGKRVQSNMGAKNHGIVMPDASPDATLNALVAAGFGAAGQRCMALSTVVFVGDPKEWETELVKRAKSLKVNAGTEPDADLGPAKERVCRLIQSGVESGARLLLDGRDIVVPGYEQGNFVGPTILSGVTEDMECYKEEIFGPVLLCMQADNLEEAINIVNRNKYGNGAAIFTSSGVAARKFQTDIEAGQVGINVPIPVPLPFFSFTGSKASFAGDLNFYGKAGVNFYTQIKTVTQQWKDLPGGSGVSLAMPTSQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C7J6</accession>
    <name>A0AAD5C7J6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_004748</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009474">
        <property type="protein sequence ID" value="KAI7735559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C7J6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7735559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="88" mass="10464" checksum="07583F6000521EBF" modified="2024-05-29" version="1">MPLERERKRARDIRWLLQWIWIVVVVAMVVTYKDYSHHSVDCSILTSKPYVRHLDRLIQPPDLVVICACRLVGRQNLLIKRLEIGDQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C7X6</accession>
    <name>A0AAD5C7X6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_014403</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009173">
        <property type="protein sequence ID" value="KAI7736862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C7X6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="115"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7736862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="189" mass="20770" checksum="180BD95769D365BF" modified="2024-05-29" version="1">MNYQSTTPRSNRPKGFMSKHMLQFALFAAFSLWLLYQIRQPTGEGGVGSKLSNDGLSTFMGRKGNVGLSKSTLNHHSGLTLEDVTNPGEEGEIPQYSKEIEVVEFRTNTVSLKEGNDQNTLSQDESSTVKKSNISFSDENGIPQHVRDKFIGKASNKTVNNTRVKSQSQSQSVIATRVGKGRNGLVEES</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C8H5</accession>
    <name>A0AAD5C8H5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_029077</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009421">
        <property type="protein sequence ID" value="KAI7735906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C8H5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.50:FF:000006">
        <property type="entry name" value="Fringe-related protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006740">
        <property type="entry name" value="DUF604"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10811">
        <property type="entry name" value="FRINGE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04646">
        <property type="entry name" value="DUF604"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7735906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="497" mass="57614" checksum="A2521AD5E9DE8BF6" modified="2024-05-29" version="1">MKQLQKDTDKIIWDQVRPNRVRNGYRKLMMYLMVFVCATYVVYTLTVFNTSNLHCSKHELVFSTRSSLQNASFSPDIVVPKKTELKHIVFGIAASAKLWERRKNYIKLWWKKDAKMRGIVWLDNHVETQPDDDGLPPVKISTDTSQFAYNNKQGHRSAIRISRIISETLRLGMNNVRWFVMGDDDTVFITDNLVRVLNKYDHNQYYYIGSLSESHLQNIFFSYGMAYGGGGFAISYPLAKALQRMQDRCIQRYPGLYGSDDRMQACMAELGVPLTKEVGFHQYDVYGNLFGLLAAHPVTPLVSLHHLDVVEPIFPEVTRVKALQRLMLPMKLDSAGLIQQSICYDSSKGWTVSVSWGFAIQVFRGILSPREVEMPSRTFLNWYRRADYTAYAFNTRPVARNPCQKAFVFYMTRVRLNSTTNMTVSQYARHRVPHPTCKWHMQDPSNLDKVIVYKRPDPQLWDRSPRRNCCRVLESKKKRLVVDVGLCREGEVTEVIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C9K5</accession>
    <name>A0AAD5C9K5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_005880</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008962">
        <property type="protein sequence ID" value="KAI7737747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C9K5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19501">
        <property type="entry name" value="RecA-like_FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000277">
        <property type="entry name" value="ATP-dependent zinc metalloprotease FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011546">
        <property type="entry name" value="Pept_M41_FtsH_extracell"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23076:SF122">
        <property type="entry name" value="AAA+ ATPASE DOMAIN, ATPASE, AAA-TYPE, CORE, PEPTIDASE M41, FTSH EXTRACELLULAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23076">
        <property type="entry name" value="METALLOPROTEASE M41 FTSH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06480">
        <property type="entry name" value="FtsH_ext"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="18"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="160"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="12">
        <location>
            <begin position="237"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7737747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="479" mass="53572" checksum="47B87FAAE469E0FB" modified="2024-05-29" version="1">MPTQNLLIFMMCRELSPLYYTIEQLFINIITLVLPLFGQRNVALCSFGIQKLPSSQYIEVPYSQLVDGIQDGSVTRVQFVENSRQIYFNKDLTEEQDVQASQADSLSTQLQVAIKKYFPNWQYHTRNVEDDKYELLRMLKDKEITYGSNRALLSASTKNFLFIFFQVAPFWIMVMLTFYQLNLQHDLGKMTKKKPSKKQSLVTFDDVKGVDAAKTELLEIVLCMKGDSKYIKLGAKPPKGVLLAGPPGTGKTLLARAVAGEAGMSFFSISGSELVEVFVGRGAARVRDLFREARKNAPSIIFIDEIDAVGGQRGRTLNCERDQTLNQLLTEMDGFEKEESVVVIAATNRPETLDSALMRPGRFSRKVRVDEPNERGRKEIFALYLKNVPFEDDKEAICEVVASLTPGLVGADLENIVRESVLLAARRGGEVVTKDDIFQAVERAKGKVYDHVAAGKPFPFGPTTQERAQGAAMGFGFPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CAZ9</accession>
    <name>A0AAD5CAZ9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_003280</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008760">
        <property type="protein sequence ID" value="KAI7738663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CAZ9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7738663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="83" mass="9236" checksum="398309A798A6295B" modified="2024-05-29" version="1">MVASSLSSIPDLCFQFSILDSPPLHYLLDISFSFASLLLSLRHLQSKILTLITVAGYLLAIDGNWYNVGWVCLVRLDFPCKCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CBL9</accession>
    <name>A0AAD5CBL9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Oil body-associated protein 1A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_007335</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the OBAP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008692">
        <property type="protein sequence ID" value="KAI7738946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CBL9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010686">
        <property type="entry name" value="OBAP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31360:SF0">
        <property type="entry name" value="OIL BODY-ASSOCIATED PROTEIN 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06884">
        <property type="entry name" value="DUF1264"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7738946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="239" mass="26869" checksum="10E3BEED3F017562" modified="2024-05-29" version="1">MEPVHASVPGEKTKITTSLVDTASAAIQTFGPPNKIHQHLCVFHMYADDMTRQVEAHHYCCNVNEDMRQCLIYDRPEPDARLIGVEYIVTEDLFLTLPDSEKPLWHSHEYEVKSGVLFMPGVPGPVERQDLDKMAKTYGKAIHFWQVDKGDELPLGLPHVMMALTRDGQLYPNLARDMENRYGVTFEKEKMNRANMEGLVHGVHPKANAAGIGLKTVLREIECKPSGISSLEPVPRAPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CBM2</accession>
    <name>A0AAD5CBM2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">M8C21_006929</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">AA19_3_7</strain>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010008855">
        <property type="protein sequence ID" value="KAI7738200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CBM2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000262">
        <property type="entry name" value="E3 ubiquitin-protein ligase XBAT33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056760">
        <property type="entry name" value="RING_XB3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24161">
        <property type="entry name" value="ANK_REP_REGION DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24161:SF85">
        <property type="entry name" value="PALMITOYLTRANSFERASE HIP14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24921">
        <property type="entry name" value="RING_XB3-XBAT31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10 11" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="repeat" description="ANK" evidence="11">
        <location>
            <begin position="44"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="11">
        <location>
            <begin position="111"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="11">
        <location>
            <begin position="177"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="11">
        <location>
            <begin position="220"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="14">
        <location>
            <begin position="319"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="456"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="475"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAI7738200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="514" mass="54781" checksum="DC48BCCD6EB159AE" modified="2024-05-29" version="1">MGNSFGCSASGERLVSAARDGDFVEAKMLLDCNPGLAKYSTFGGLNSPLHFAAAKGHNDIVALLLDNGADVNSRNYCGQTALMQACRYGHWEVVQTLLLFRCNVMRADYLSGRTALHFAAVNGHVRCIRLVVADFVPSAPFEAIDAHANGDGSNGHSPKNKFDHSALVKFVNKAADGGITALHMAALNGYADCVQLLLDLHANTSVVTFHYGTSMDLIGAGSTPLHYAACGGNLKCCQILLARGASRLTLNCNGWLPLDVARMWGRHWLEPLLAPNSDLAIPVFPPSNYLSLPLMSVLNIARECGLQSSGTLSDDTDICAVCLERSCTVAAEGCRHELCVRCALYLCSTSNNPSELLGPPGSIPCPLCRHGIISFTRLPGSPAKEIKLHLSLGFCTPCMLHPREPTESTPASTPEISKNRVASISPELFCPVTCSPFPSVAIPLCTCSDGTCPSSQSRDDEAQGGSDGSPSHRTTSGEDGKLGSLRLEKTSCSSMFWGRRSCSREQQCNSEINA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CCK8</accession>
    <name>A0AAD5CCK8_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_010755</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008628">
        <property type="protein sequence ID" value="KAI7739412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CCK8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="102"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="177"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7739412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="212" mass="23042" checksum="6DF1E1EFB038921C" modified="2024-05-29" version="1">MEDEQGRKPTPITAAEEFWSGIRADTERRRAAALADIAEGKGPCVQELGSASATTGRKAQVKGKRPCVVNPSSSSATTVMDETLDEDIRFLSELISSRYASTGKHSFGTKAPGSPKSPNYVDLSEYPPDTEIDYELHLEADGRRFVERVPRWAREKMQLMFQDPCDPEEDHSLATIKPEDIGSDGMHAVGGGQALGGNNNPNPPALVWVVSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CD60</accession>
    <name>A0AAD5CD60_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="24">Photosystem II protein D1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">M8C21_003945</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="22">AA19_3_7</strain>
            <tissue evidence="22">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 19">Belongs to the reaction center PufL/M/PsbA/D family.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008627">
        <property type="protein sequence ID" value="KAI7739447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CD60"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009523">
        <property type="term" value="C:photosystem II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016168">
        <property type="term" value="F:chlorophyll binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009772">
        <property type="term" value="P:photosynthetic electron transport in photosystem II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.85.10">
        <property type="entry name" value="Photosystem II protein D1-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055266">
        <property type="entry name" value="D1/D2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036854">
        <property type="entry name" value="Photo_II_D1/D2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000484">
        <property type="entry name" value="Photo_RC_L/M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055265">
        <property type="entry name" value="Photo_RC_L/M_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33149:SF12">
        <property type="entry name" value="PHOTOSYSTEM II D2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33149">
        <property type="entry name" value="PHOTOSYSTEM II PROTEIN D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00124">
        <property type="entry name" value="Photo_RC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00256">
        <property type="entry name" value="REACTNCENTRE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81483">
        <property type="entry name" value="Bacterial photosystem II reaction centre, L and M subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00244">
        <property type="entry name" value="REACTION_CENTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0148">Chlorophyll</keyword>
    <keyword evidence="14" id="KW-0157">Chromophore</keyword>
    <keyword evidence="11" id="KW-0249">Electron transport</keyword>
    <keyword evidence="16" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="17 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-0602">Photosynthesis</keyword>
    <keyword evidence="18" id="KW-0604">Photosystem II</keyword>
    <keyword evidence="7" id="KW-0934">Plastid</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="21" id="KW-0732">Signal</keyword>
    <keyword evidence="8 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042043445" description="Photosystem II protein D1" evidence="21">
        <location>
            <begin position="25"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="74"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="105"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="237"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU004331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="KAI7739447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="24">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="273" mass="29976" checksum="E0EB8FFCA8DA1E13" modified="2024-05-29" version="1">MISTLLTATSVFIIAFIAAPPVDIDGIREPVSGSLLYENNIISGVIIPTSAAIGSHFYPIWEATFVDEWLYNGGPYELIVLHFLLGVACYMGREWELSFRLGMRPWIAVAYSAPAAAATVVFLIYPIGQGSFSDCMPLGISGTFNLMIVFQAEHNILMHPFHMLGVASVFGGSLFSAMHGSLVTSSLIRETTENESANEGYRFGQEEETYNIVSAHGYFGRLIFQYASFNNSRSVHFFLAAWLVVGIRFTALGISTMAFNLNGFNFNQSVVDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CDS6</accession>
    <name>A0AAD5CDS6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_000697</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase A1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008597">
        <property type="protein sequence ID" value="KAI7739628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CDS6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05476">
        <property type="entry name" value="pepsin_A_like_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000028">
        <property type="entry name" value="Eukaryotic aspartyl protease family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034161">
        <property type="entry name" value="Pepsin-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032799">
        <property type="entry name" value="TAXi_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032861">
        <property type="entry name" value="TAXi_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13683">
        <property type="entry name" value="ASPARTYL PROTEASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13683:SF768">
        <property type="entry name" value="EUKARYOTIC ASPARTYL PROTEASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14541">
        <property type="entry name" value="TAXi_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14543">
        <property type="entry name" value="TAXi_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase A1" evidence="7">
        <location>
            <begin position="92"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="110"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7739628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="472" mass="50917" checksum="01CE8BD61EAC6EB9" modified="2024-05-29" version="1">MDLVMIRSRKRGGGGFVGVLVIIGFLLLLQLASANVVFQVHHKFATTTTSSSKRPSLLTHFKAHDSARHRRILSAVDLPIGGDGSPTSSALYFTKIRIGTPPKDYHVQVDTGSDLLWVNCHGCAKCPKKSDLGISLALYDPEASSTAKMVECNQDICMSTLSDPSNDCKAGMYCSYSVKYGDGSSTAGFFVRDTVQLDRVSGNLQTTFLNGSVAFGCGSQQSGQLGSSSQALDGILGFGQANSSLLSQLASAKKVKRIFSHCLDGSQGGGIFAIGEVAQPKVQTTPMIPEAHYNVELKQIEVGREVLDLPTDTFDVGGKQGTIIDSGTTLAYLPDLAYKEVLEKIGAAQPNIQPHIVEQQFTCYKYSGDVDEGFPVIQFLFENSLYLKVHPHQYLFEVQDDEWCVGFQDSELQSQGKDVTLLGGSSSIKVKDEESGKEYTLGAHNLSGGDRIGTSTGIIMLMFLVIKLINLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CDV3</accession>
    <name>A0AAD5CDV3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein RDM1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_021096</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008720">
        <property type="protein sequence ID" value="KAI7738799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CDV3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000419">
        <property type="term" value="C:RNA polymerase V complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080188">
        <property type="term" value="P:gene silencing by siRNA-directed DNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.690">
        <property type="entry name" value="RDM1 protein domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015270">
        <property type="entry name" value="RDM1_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036319">
        <property type="entry name" value="RDM1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36366">
        <property type="entry name" value="PROTEIN RDM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36366:SF1">
        <property type="entry name" value="PROTEIN RDM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09187">
        <property type="entry name" value="RdDM_RDM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109920">
        <property type="entry name" value="Hypothetical protein At3g22680"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7738799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="172" mass="19723" checksum="B37C959AE976415D" modified="2024-05-29" version="1">MKRTMTSQPLDISSDDDDDPAPSNGTKHLAVKRPKTELTVINLAKKVVGEELLIRSAKMYQEYMKQIPIPAERGSVISFTSWSGLAKSMKQMYRQPLHYLTNIRVKEWDRMRIDEAGKDEPLDAIIHPCKAETNIWLMEEVHRLTASHHFLAKLWSADPMYNASIDSVFPQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CE90</accession>
    <name>A0AAD5CE90_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_029315</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008543">
        <property type="protein sequence ID" value="KAI7739913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CE90"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7739913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="163" mass="18752" checksum="6B1567673C3F089C" modified="2024-05-29" version="1" fragment="single">HYNQAPEENLNPSFSKEEGFLLCFLPTLNMGILSQQPDKPSSSFSALSTTRNTKEEGLHLLFLHSNCLITISVCPFEIYTLDDNSEFRVMVPMTHLPPWSLLSFRYITDDHRLHSTCPSLINKKRNLGHDWLRAVTRGSLLASFDQKGLNDFNRMHQFSNLRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CGL9</accession>
    <name>A0AAD5CGL9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">QLQ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_005929</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008412">
        <property type="protein sequence ID" value="KAI7740540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CGL9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014978">
        <property type="entry name" value="Gln-Leu-Gln_QLQ"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08880">
        <property type="entry name" value="QLQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00951">
        <property type="entry name" value="QLQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51666">
        <property type="entry name" value="QLQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="QLQ" evidence="4">
        <location>
            <begin position="115"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7740540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="152" mass="16543" checksum="A01930D8976AA469" modified="2024-05-29" version="1" fragment="single">MSSNEYSTENIGCRNENSGKAPSQAAECDFGLALNMQTTNPTKKINFIFDHPSLYHHPQHRASIDFGGGRDRGGGSGGGGGRRLMFDNSRANSNQIAAGTSFINPTGGGEMQKMLFTASQLQEFENQTLIYKHIMSSNPIPPQLMLPLSTQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CHG1</accession>
    <name>A0AAD5CHG1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_019861</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008163">
        <property type="protein sequence ID" value="KAI7741584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CHG1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46398">
        <property type="entry name" value="ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46398:SF5">
        <property type="entry name" value="ALPHA_BETA-HYDROLASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7741584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="216" mass="24487" checksum="98F1DC4E497DF463" modified="2024-05-29" version="1" fragment="single">MFWLKRMTFNSSIKLYSPSLHLIFIMHVKSPPPFPGLFVSIQSFPCILCGTCLKETCTLQEQMLQDPRRLYAPGRLYHLIVRKPFGVGRISPVMKTAVPIDWRFEHMVLSCHVLSDHSIALMEPEFSNALDFLNCLNIESYNFLGFAAAEEYCTSRVTETIQRYSASMQYSCRRSTITLHVELEQVVADFGGKPAAMVIGMGYVTNSAILPVLVKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CI14</accession>
    <name>A0AAD5CI14_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Calcium uniporter protein C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_033041</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MCU (TC 1.A.77) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008323">
        <property type="protein sequence ID" value="KAI7740914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CI14"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990246">
        <property type="term" value="C:uniplex complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015292">
        <property type="term" value="F:uniporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036444">
        <property type="term" value="P:calcium import into the mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051560">
        <property type="term" value="P:mitochondrial calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006769">
        <property type="entry name" value="MCU_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039055">
        <property type="entry name" value="MCU_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13462:SF52">
        <property type="entry name" value="CALCIUM UNIPORTER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13462">
        <property type="entry name" value="CALCIUM UNIPORTER PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04678">
        <property type="entry name" value="MCU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="228"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="254"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="Calcium uniporter protein C-terminal" evidence="11">
        <location>
            <begin position="154"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF04678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7740914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="342" mass="39155" checksum="4ACF332CECCB7088" modified="2024-05-29" version="1">MALRRTITKRLIEDQPLIITNLLPHKTYPHHKLLTSPHKPSSGSTGFFRRFFQRNQSSTMSLPEMLAFPVGEKLREKLNINSYGLRSPVTEWLPEQPVKPVNVDSVGISVNDAKKIIRLSQLQKVRHALKQITMNSISYDEFVTVCIDTCRNHEQGVEFATMLDQAGDVIVFGNVVFLRPDQLAKSMEKLISQSIAVPNDARKQELEKLEMQKALIDKKSLSQVRAELYCGLGYLVMQTVAFMRLTFWELSWDVMEPICFFVTSFHFVLAYVFFIRTSKEPSFEGYFQRRFRTKQRKLMEVHGFDAQKYDDLCKVFYATNLSRGAGGSQDLSSGASSNVVFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CJ64</accession>
    <name>A0AAD5CJ64_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_012216</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007887">
        <property type="protein sequence ID" value="KAI7742808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CJ64"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23798">
        <property type="entry name" value="UBCc_UBE2I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000038">
        <property type="entry name" value="SUMO-conjugating enzyme SCE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="5">
        <location>
            <begin position="5"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="3">
        <location>
            <position position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7742808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="160" mass="17844" checksum="DFB0D406E2519193" modified="2024-05-29" version="1">MAAGVAVGRLTEERKAWRKNHPYGFVAKPQSVADGSVNMMVWQCIIPGKAGTDWEGGYYPLTLTFSEDYPSKPPICKFPQGFFHPNVYSSGMVCLSILSESSGWRPAITVKQILVGIQDLLDTPNPASPAQNEAYGFFVKDLAEYKRRVRQQGKRYPPKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CJB2</accession>
    <name>A0AAD5CJB2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_010407</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007852">
        <property type="protein sequence ID" value="KAI7742936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CJB2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36338">
        <property type="entry name" value="OS02G0495900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36338:SF1">
        <property type="entry name" value="OS02G0495900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="24"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7742936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="121" mass="14004" checksum="232963F6118F2588" modified="2024-05-29" version="1">MSILWEKSSAWRWLVRKTRDSKPFFFGFATVCAVVPGVVGYYVMQITNSRNDQLEAQLRRDARPDTLLMGKVNKERLGEFLGELQRKEDTNDRYVAALKGETLTRNPYIRIQPVNPPQADK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CK55</accession>
    <name>A0AAD5CK55_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">TNase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_005800</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007832">
        <property type="protein sequence ID" value="KAI7743054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CK55"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035437">
        <property type="entry name" value="SNase_OB-fold_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016071">
        <property type="entry name" value="Staphylococal_nuclease_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002071">
        <property type="entry name" value="Thermonucl_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12302">
        <property type="entry name" value="EBNA2 BINDING PROTEIN P100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12302:SF3">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00565">
        <property type="entry name" value="SNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00318">
        <property type="entry name" value="SNc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50199">
        <property type="entry name" value="Staphylococcal nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01284">
        <property type="entry name" value="TNASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50830">
        <property type="entry name" value="TNASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TNase-like" evidence="4">
        <location>
            <begin position="153"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7743054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="338" mass="38402" checksum="088644A9129E936A" modified="2024-05-29" version="1">MGNALRFLYGQCCSPSPQTTTDDVVLGHHGVSSSTVGVSALAQHLYHFEITSQVPDRLSVHVVSSKKAQSNWYKKLSKAWRESKPPPSTPEEASRLVVQTLQRHQVPDVQGLLSFYGLPIPYSLDDLTADEAPTVVDGLKFELHTLPVDGKTVPDGDGVNVYVNTTDARESSLVPHDVHWAAVERSQARAQRNYNMADALHKQITDAGYRVLNINDEEILARKYRIRLRGIDAPENQQPYGKEAKDELVKIVDGKCLQIQVFDEDRYGRFVGDVYCNGHLVQKRMLKKGLAWHYTAYDKRPQFAKWEKEARAKGIGLWALENPEMPWEWRRNHRGNRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CK60</accession>
    <name>A0AAD5CK60_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Calponin-homology (CH) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_003223</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Interacts with F-actin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007742">
        <property type="protein sequence ID" value="KAI7743342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CK60"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005884">
        <property type="term" value="C:actin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032432">
        <property type="term" value="C:actin filament bundle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051017">
        <property type="term" value="P:actin filament bundle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051639">
        <property type="term" value="P:actin filament network formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21296">
        <property type="entry name" value="CH_AtFIM_like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21299">
        <property type="entry name" value="CH_AtFIM_like_rpt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21302">
        <property type="entry name" value="CH_AtFIM_like_rpt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000045">
        <property type="entry name" value="Fimbrin-1 isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000041">
        <property type="entry name" value="Fimbrin-2 isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000031">
        <property type="entry name" value="Fimbrin-2 like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000034">
        <property type="entry name" value="Fimbrin-2 like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001589">
        <property type="entry name" value="Actinin_actin-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039959">
        <property type="entry name" value="Fimbrin/Plastin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19961:SF62">
        <property type="entry name" value="FIMBRIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19961">
        <property type="entry name" value="FIMBRIN/PLASTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00020">
        <property type="entry name" value="ACTININ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="8">
        <location>
            <begin position="122"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="8">
        <location>
            <begin position="266"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="8">
        <location>
            <begin position="391"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="8">
        <location>
            <begin position="513"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="629"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="630"/>
            <end position="646"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7743342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="687" mass="76923" checksum="2577B462DF1BA222" modified="2024-05-29" version="1">MSKFEGVLVSDHWLQSQFTQVQLRSLKSKFIASKNQSGVVTASDLPVLLAKLKPFSEMFKENEIADLLDKSGLDMSNGIDFEGFLRAYLNLHSQAAAKLGNSNNSSSFLKATTTTLLHTIDESEKESYVAHINSYLRDDPFMKQFLPIDPTTNALFELARDGVLLKLINVAVPNTIDERAINTKRVLNPWERNENHTLCLNSAKAIGCTVVNIGNQDLVEGRPHLVLGLISQIIKIQLLADLNLRKTPQLVELVEDNNDVEELMGLAPEKVLLKWMNFHLKKAGYKKPVTNFSSDLKDGEAYAYLLNVLAPEHCSPATLDTKDPTERANLVLEHAEKMDCKRYLAPKDIVEGSTNLNLAFVAQIFHQRNGLSTDNKKISYAEMMTDDEEMSRDERCFRLWINSLGISSYVNNLFEDVRNGWTLLEVVDKVSPGSVNWKHATKPPIKMPFRKVENCNQVIKIGKQLKFSLVNVAGNDFVQGNKKLILAFLWQLMRFNMLQLLKNLRSRSSQGKEITDADILKWANKKVKNTGRMSQMESFKDKSLSNGIFFLELLSAVEPRVVNWNLVTKGESEEEKKLNATYIISIARKLGCSIFLLPEDIMEVNQKMMLMLAASIMYWSLLQSTDDSESSPSSIAATPEASPAPSVNGDEGSEFSMSAEIANLSIDDAASDTTVSSIQENNDTPLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CLI6</accession>
    <name>A0AAD5CLI6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_010924</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007578">
        <property type="protein sequence ID" value="KAI7744136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CLI6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26312:SF168">
        <property type="entry name" value="OS06G0606700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26312">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7744136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="251" mass="27356" checksum="17C9B721AFC609FE" modified="2024-05-29" version="1">MLLRSSSTPSLTSCFQHQNHKPLTPSQTQQSFIHQNINPNPQIISLHSPKNITRSSSEGDLVTLSRSLCNNININPHNSDHHMMFSWSELDVDEGCHGGSSGGGKICGGGGGDEGYYYNGDGNGMDGYYRRMVEGDPSNSLVLGNYAKYLKEVRGDVLKAEEYCSRAILANPCDGTALSMYADLIWETQNDASRAQSYFDQAVNASPDDSYVMAAYARFLWDADEEESDANLTAPGFLHEASLQLPIAAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CM29</accession>
    <name>A0AAD5CM29_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_031023</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007701">
        <property type="protein sequence ID" value="KAI7743655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CM29"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7743655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="127" mass="14323" checksum="46A43508621A87D7" modified="2024-05-29" version="1" fragment="single">CSLQLEYLQHKMLMAVCLSFEGHFKDSDRISISKLIKLISREVKEVIISFKNEKRRSHYDATALEGTGAVTVKESITFFLERIRFGLEAVWSSSGAICSAWKKRDALAIFEWSGESASMALKAAVWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CMG0</accession>
    <name>A0AAD5CMG0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Myosin-15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">M8C21_020567</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">AA19_3_7</strain>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family. Plant myosin class XI subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007773">
        <property type="protein sequence ID" value="KAI7743211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030048">
        <property type="term" value="P:actin filament-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd23767">
        <property type="entry name" value="IQCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01384">
        <property type="entry name" value="MYSc_Myo11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.820:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.190">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.240.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002710">
        <property type="entry name" value="Dilute_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036018">
        <property type="entry name" value="MYSc_Myo11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004009">
        <property type="entry name" value="SH3_Myosin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140">
        <property type="entry name" value="MYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140:SF781">
        <property type="entry name" value="MYOSIN-15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01843">
        <property type="entry name" value="DIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02736">
        <property type="entry name" value="Myosin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM01132">
        <property type="entry name" value="DIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51126">
        <property type="entry name" value="DILUTE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="4 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="6 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8 10" id="KW-0505">Motor protein</keyword>
    <keyword evidence="7 10" id="KW-0518">Myosin</keyword>
    <keyword evidence="3 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Myosin motor" evidence="14">
        <location>
            <begin position="68"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="domain" description="Dilute" evidence="13">
        <location>
            <begin position="1035"/>
            <end position="1347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="10">
        <location>
            <begin position="619"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1159"/>
            <end position="1178"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="888"/>
            <end position="950"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1169"/>
            <end position="1178"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="162"/>
            <end position="169"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAI7743211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1347" mass="152951" checksum="3B93D0EE960F4F1A" modified="2024-05-29" version="1">MMMMSNINATMSLRNGSKVWIEDKDTAWVAGEVTAFAGNQVQLVTDSGKQVLALAEKLLLRDADADHGGVDDMTKLAYLNEPGVLDNLKKRYALNEIYTYTGSILIAVNPFTKLPHLYNVHMMEQYKGAPFGELSPHVFAVADASYRAMMGDAKSQSILVSGESGAGKTETTKLIMQYLTYVGGRAASDDRTVEQQVLESNPLLEAFGNARTVRNDNSSRFGKFVEIQFDANGRISGAAIRTYLLERSRVVQITDPERNYHCFYQLCASGRDADNYKLGHPSNFHYLNQSTVYELEGVSSAEEYLKTRRAMDIVGISNDEQEAIFRTLAAILHLGNIEFSPGKEHDSSMVKDEKSNFHLQTAANLFMCDAKLLLATLCTRSIQTREGIIVKALDCDAAVASRDALAKTIYARLFDWLVEKINRSVGQDSNSRMQIGVLDIYGFECFKHNSFEQFCINFANEKLQQHFNEHVFKMEQEEYRKEAITWSYIEFIDNQDVLDLIEKKPIGVIALLDEACMFPKSTHETFANKLFQNLRAHPRLGKAKFSETDFTISHYAGKVNYQTDSFLDKNRDYVVIDHCNLMSSSKCGFIAGLFPALPEESSRSSYKFSSVASRFKQQLQALMDTLSSTEPHYVRCVKPNSVNRPQKFENQSVLHQLRCGGVLEAVRISLAGYPTRKTYHEFVDRFGIIAMEVMDGSYDGRGMTEKILQKLKLENYQLGKTKVFLRAGQIGILDSQRAGVLDYAAKRIQLRLRTYIAHKNFTLKRAAAISFQAYCRGYLARVTYAAKRKDAAAILIQKYIRGWLLRNAYTQKYLSALLLQASIRGFTTRQRFLHIREHRAATVIQARWRMFKVRSAFQHRHRDITKIQCLWRRKLAKRELRRLKKSSLSTLEEKNSSLQSQLTEAKEDASNNLKKLREVEKTCLQLQQKLKSFEEKFSSLEKENLVLRQKTFSTPLKGNWPSSTKPYIEKLSGALALPFSERKSIYETPTPTKSGSLISQGVTDSRRRTXXXXSVIYKCLLHWHAFDSERTAIFDFIIEKINTVLKEGDEGVTLPYWLSNASALLCLLQRNFRSNGFLTPISQRSGVSTLPNGRAAQSFTPHKYIGFEDGISPMEARYPAILFKQQLTACVEKIFGLIRDNLKKEISPLLASCIQAPKNQRVHGGKPSRSPSGIPQQAAGSQWEKIIEFMDSLMERLRGNHVPSFFIRKLTTQVFSFINISLFNSLLLRRECCTFSNGEYVKSGLAELEKWIANAKEEVVAEMREMLSKDTQNLTSNSFLLDDDLSIPFSTEDIYMTIPAIDPTDIEAPAFLSEYPAAQFLLPNPNLGQVLDVKAKFMYMKYYNLTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CMX4</accession>
    <name>A0AAD5CMX4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">V-type proton ATPase subunit a</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_004123</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Essential component of the vacuolar proton pump (V-ATPase), a multimeric enzyme that catalyzes the translocation of protons across the membranes. Required for assembly and activity of the V-ATPase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the V-ATPase 116 kDa subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007700">
        <property type="protein sequence ID" value="KAI7743675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CMX4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000220">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051117">
        <property type="term" value="F:ATPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002490">
        <property type="entry name" value="V-ATPase_116kDa_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026028">
        <property type="entry name" value="V-type_ATPase_116kDa_su_euka"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629:SF98">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629">
        <property type="entry name" value="VACUOLAR PROTON ATPASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01496">
        <property type="entry name" value="V_ATPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001293">
        <property type="entry name" value="ATP6V0A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 9" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="352"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="397"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="477"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="502"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="557"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="677"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="30"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7743675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="740" mass="84215" checksum="106C78E340A70B81" modified="2024-05-29" version="1">MARKLRFFRDQMYKAGVSASKFAYGSVITLDELEIKLGELESELIEMNANSEKLQQAYNELLEYRLVLKKMGEVYNSAQRKATARHREQENRRVVEGAIDSPLLLEQEMTIDSSKQVKLGYVSGLVPRDKSMAFERILFRATRGNVFLKQESIEEPVIDPVSGQKVEKNVFIVFHSGERAKSKVLKICDAFGANRYPFTDDIVKQHQMIVEVAGKLSELKTTIDVGQHHWGSVLQAISDQYDQWKNMVKKEKSIYHTLNMLSFDVTKKCLVAEGWCPVSATDEIQKTLSQATTDSNSQIGAIFEILHSKEAPPTYFCTNKFTYAFQEIVNAYGVAKYQEANPGVFTVVTFPFLFAVMFGDWGHGICLFLVTLILILREKTYSRQKLGDIMEMAFSGRYVIIMMSIFSIYTGVIYNEFFSVPYEIFGPSAYACRDSSCSDATTVGLIKVRKTYPFGVDPTWHGSRSELPFLNSLKMKMSILLGVAQMNLGIILSYFNAKYQFVPQIIFLNSLFGYLSLLIIVKWCTGSQADLYHVMIYMFLSPMDDLNDNQLFMGQKYLQLLLLFLALIAVPWMLIPKPFLLKKQHEERHQGEAYVPLSTVDENLEPALTDGSHGGHEEFQFSEVVVHQLIHTIEFVLGSVSNTASYLRLWALSLAHSELSKVFFEKVLVLAWGYDSVLIRIIGGIVFIFATVGVLLVMETLSAFLHALRLHWVEFQNKFYEGNGYRFEPFSFALIGDDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CPQ9</accession>
    <name>A0AAD5CPQ9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Hydroxymethylglutaryl-CoA synthase</fullName>
            <shortName evidence="5">HMG-CoA synthase</shortName>
            <ecNumber evidence="5">2.3.3.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">3-hydroxy-3-methylglutaryl coenzyme A synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_008228</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the condensation of acetyl-CoA with acetoacetyl-CoA to form HMG-CoA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>acetoacetyl-CoA + acetyl-CoA + H2O = (3S)-3-hydroxy-3-methylglutaryl-CoA + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10188"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43074"/>
            <dbReference type="ChEBI" id="CHEBI:57286"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.3.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the thiolase-like superfamily. HMG-CoA synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.10" evidence="5"/>
    <dbReference type="EMBL" id="JAMZMK010007208">
        <property type="protein sequence ID" value="KAI7745529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CPQ9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004421">
        <property type="term" value="F:hydroxymethylglutaryl-CoA synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006084">
        <property type="term" value="P:acetyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010142">
        <property type="term" value="P:farnesyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016126">
        <property type="term" value="P:sterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00827">
        <property type="entry name" value="init_cond_enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000008">
        <property type="entry name" value="3-hydroxy-3-methylglutaryl coenzyme A synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000590">
        <property type="entry name" value="HMG_CoA_synt_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013746">
        <property type="entry name" value="HMG_CoA_synt_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013528">
        <property type="entry name" value="HMG_CoA_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010122">
        <property type="entry name" value="HMG_CoA_synthase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01833">
        <property type="entry name" value="HMG-CoA-S_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43323">
        <property type="entry name" value="3-HYDROXY-3-METHYLGLUTARYL COENZYME A SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43323:SF2">
        <property type="entry name" value="HYDROXYMETHYLGLUTARYL-COA SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08540">
        <property type="entry name" value="HMG_CoA_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01154">
        <property type="entry name" value="HMG_CoA_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01226">
        <property type="entry name" value="HMG_COA_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="5" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="5" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Hydroxymethylglutaryl-coenzyme A synthase N-terminal" evidence="6">
        <location>
            <begin position="3"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="Hydroxymethylglutaryl-coenzyme A synthase C-terminal" evidence="7">
        <location>
            <begin position="176"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="3">
        <location>
            <position position="84"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="3">
        <location>
            <position position="118"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="3">
        <location>
            <position position="248"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="253"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR610122-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR610122-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7745529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="456" mass="50377" checksum="D844B2FE3E8C3ABF" modified="2024-05-29" version="1">MAPQNVGILAMEIYFPPTCIQQDILEDFDGVSKGKYTIGLGQDCMAFCSEVEDVISMGLTAVTSLLEKYGVDPRQIGRLEVGSETVIDKSKSIKTFLMNVFEDCGNTDIEGVDSTNACYGGTAALFNCVNWVESNSWDGRYGLVVCTDSAVYAEGPARPTGGAAAIAMLIGPDAPIAFESKLRASHMSHVYDFYKPDLASEYPVVDGKLSQTCYLMALDSCYNGYCQKYEKLEGKQFSIADAEYFVFHSPYNKLVQKSFARVVFSDVVRNASYVDESANEKLGQFTSLTGDESYQNRDLEKASQQVAKPYYDKKVQPGTLINKQVGNMYTASIYAAFASLIHNKHSSLDGNRVMMFSYGSGSSATMFSLHLSEGKAPFSLSNIAKVMNVDEKLKRRTELAPEKFVQLMKVMEHRYGGKDFVTSKDTSGLAPGTYYLTEVDSKYRRFYAKKTSEVPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CQ99</accession>
    <name>A0AAD5CQ99_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_022017</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007505">
        <property type="protein sequence ID" value="KAI7744546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CQ99"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7744546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="62" mass="7247" checksum="82B0982013911B94" modified="2024-05-29" version="1" fragment="single">MKGTLDLFGAATEKFTCERRQSGEIDHLRSHNPKIRKFGKHYVFILPLEQDLIPLMFQKNSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CT81</accession>
    <name>A0AAD5CT81_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Alcohol dehydrogenase-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_026828</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006673">
        <property type="protein sequence ID" value="KAI7747818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CT81"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032440">
        <property type="term" value="F:2-alkenal reductase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000121">
        <property type="entry name" value="Prostaglandin reductase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045010">
        <property type="entry name" value="MDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43205">
        <property type="entry name" value="PROSTAGLANDIN REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43205:SF7">
        <property type="entry name" value="PROSTAGLANDIN REDUCTASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alcohol dehydrogenase-like C-terminal" evidence="2">
        <location>
            <begin position="90"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7747818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="268" mass="29507" checksum="E63E607563280923" modified="2024-05-29" version="1" fragment="single">PISGYGVAKVLDSTHPDFNKGDLVSGITNWEEYSLITNPESLIKIQHTDVPLSYYTGILGMAGLTAYACFYEICAPKKGDTVFVSAASGAVGQLVGQFAKLSGCYVVGSAGSKEKVDLLKNDFGFDEAFNYKEEQDLDIALKRYFPNGIDIYFENVGGKMLDAVLCNMRVHGRIAVCGMISQYNLNRPEGINNLMFLILKRIRMEGFLVVDYHHLYPKLFEMILPLIREGKITYVEDTVEGLDNAPAALVGLFSGRNVGKQVVAIAHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CTK1</accession>
    <name>A0AAD5CTK1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peroxygenase-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_004690</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the caleosin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007011">
        <property type="protein sequence ID" value="KAI7746286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CTK1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007736">
        <property type="entry name" value="Caleosin-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31495">
        <property type="entry name" value="PEROXYGENASE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31495:SF37">
        <property type="entry name" value="PLANT SEED PEROXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05042">
        <property type="entry name" value="Caleosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="91"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7746286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="236" mass="26713" checksum="ACF3D1C7A07CBD6B" modified="2024-05-29" version="1">MEVSDEPVAMATVAPMAPVTAQRNVRTDLESSIPKPYMARAMATPDAQHPDGTPEHEARGMSTLQQHSAFFDQDNNNIVYPWETYTGMRKVGFNIVISGLAALIINVGMSYPTLPGWLPSPLFPIHINNIHRSKHGSDSGTYDTEGRYMPVQFENMFSKYAKTKPDKLTFIEMWNMTEGNRVAFDFFGWFAAKLEWIILYVLAKDEEGMLPKEAARGCYDGSLFNYFAKMNKNKTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CTL2</accession>
    <name>A0AAD5CTL2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_017528</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ARG7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006712">
        <property type="protein sequence ID" value="KAI7747659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CTL2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009733">
        <property type="term" value="P:response to auxin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003676">
        <property type="entry name" value="SAUR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31374">
        <property type="entry name" value="AUXIN-INDUCED PROTEIN-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31374:SF29">
        <property type="entry name" value="SAUR-LIKE AUXIN-RESPONSIVE PROTEIN FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02519">
        <property type="entry name" value="Auxin_inducible"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7747659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="98" mass="11582" checksum="3A430F44D1F197E5" modified="2024-05-29" version="1">MELFYEVRQWNQVPKGFLAVNVGEAEGEKQRFVVPVMWFNHPLFVELLKVAEDEYGFEQKGTITIPCNVHHFCTTISSITHHYRKQQQYFACFKVWVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CXR8</accession>
    <name>A0AAD5CXR8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">MBD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_027532</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006268">
        <property type="protein sequence ID" value="KAI7749867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CXR8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.890.10">
        <property type="entry name" value="Methyl-cpg-binding Protein 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="158"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="165"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7749867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="337" mass="38777" checksum="0AECC1EDA6E2EBF7" modified="2024-05-29" version="1">MVNNSLENQSFIPTGKEIVLYDDKEAQNWRKCPNNIITAEPLTWWKPQEVSGAFEIISRRQKDETTIDPQELLINLKGWTVEKRKRIENNGKYRVDKTYRHPKSKRILRSLVEVKKFIEENSFIGKTSDNGEGGSQDATSNMYLQIFKRWEKTYGSGAQETFNNSSKEGSSSWSEQHEAVRMPKTTCGATLKSHDTLDLSLHLHRPFHYQERMDKKWNVVPQAEENNDLSNQQVFNNLTRSSEQECVRTFGVELPPCDVFGASSSSTNITSLDEMKLLVQSQVEAQIESRVAAQLAERISHYEKTQREMAQSIESMQRLLVLQGMLPPSQFPNTRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CYR8</accession>
    <name>A0AAD5CYR8_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_022089</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">May transport inorganic phosphate (Pi).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SYG1 (TC 2.A.94) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006152">
        <property type="protein sequence ID" value="KAI7750449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CYR8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000822">
        <property type="term" value="F:inositol hexakisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016036">
        <property type="term" value="P:cellular response to phosphate starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14476">
        <property type="entry name" value="SPX_PHO1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004342">
        <property type="entry name" value="EXS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034092">
        <property type="entry name" value="PHO1_SPX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004331">
        <property type="entry name" value="SPX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10783:SF112">
        <property type="entry name" value="EXS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10783">
        <property type="entry name" value="XENOTROPIC AND POLYTROPIC RETROVIRUS RECEPTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03124">
        <property type="entry name" value="EXS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03105">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51380">
        <property type="entry name" value="EXS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51382">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="368"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="401"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="464"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="522"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="564"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="domain" description="SPX" evidence="13">
        <location>
            <begin position="3"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="EXS" evidence="12">
        <location>
            <begin position="460"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="143"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="631"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00043939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7750449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="631" mass="72768" checksum="5334FD677E3E26E6" modified="2024-05-29" version="1" fragment="single">LAMKFGKEFASQMVPEWQEAYMNYNYLKTLLKQILIFHQRKTRSPEITKNSRSVMLSKEASLKRNLTLFRAFSGLTGGYGNSSPRKDKEDDVILVNVMQEADEEQGDDGDQTYKTIFLRSSEEGGDLEHIFFERLDDEFNKVIRFYKSKVEEVVMQAEELSNQMDALIALRIKVINPTAAAAIAVASGSHPLEVIQEEKQEKRTEAAKGYKMASLDVLNHVKINAAPKTLSALKNVFKCLKFDMHFNKNEVKVAEEKLEQAFVEFHQKLRFLKNYAFLNQLAFSKIMKKYDKITSRNASYAYMKMVEESYLSQSEEVAKIIDRVEAVFIKHFCNGNRPQGMDTVRPKTKREKHRITFLVGTELGFKEVLLLGFGLSVLTLAAAVILTAVCPFDIFYRANRFFLLVCLWHCICAPLYAVTLPDFFLADQFTSQVQLLRNLQFYVCYYGWGDFKRRNAETCNNSKVYDVLFIVTAVIPYWIRLLQCIRRWNDGRDSTQALNGLKYFSTIVALVTRTIYSQKGGIAMKVISAFTSGVATIFSTYWDIVMDWGLLTKNSHNPLLRDKLILPSRSIYFIAMVLNVILRLAWMQTVLDFHELPFLHKNALTAIFANLEIIRRGIWNFFSYEEGYKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CZG1</accession>
    <name>A0AAD5CZG1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_015084</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005956">
        <property type="protein sequence ID" value="KAI7751163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CZG1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11072">
        <property type="entry name" value="CYP71-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000054">
        <property type="entry name" value="Cytochrome P450 84A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053062">
        <property type="entry name" value="CYP450_84A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47945">
        <property type="entry name" value="CYTOCHROME P450 84A1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47945:SF5">
        <property type="entry name" value="CYTOCHROME P450 84A1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 9 10" id="KW-0349">Heme</keyword>
    <keyword evidence="7 9 10" id="KW-0408">Iron</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 9 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="9">
        <location>
            <position position="439"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7751163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="502" mass="56822" checksum="B235C35F50B630AF" modified="2024-05-29" version="1">MDFMSIVLYVIVPLLTIFLLSRPRRKPLPPGPKGWPIIGNMLMMDQLTHHGLANLAETYGGIFHLKMGFSHTVAVSSPEIVRQVLQVQDNIFSNRPATIAIAYLTYDRQDMAFANYGPFWRQMRKLCVMKLFSRKRAESWDSVRDEVDSMIKITADSSGSPVNLGELVFGLTHDIIYRAAFGSISHEGKEEFIRILQEYTKLFGTFNLADFIPWLGFIDPAGLNTRLPKARATLDGFIDKIIDEHLKKKKTADDADRDMVDEMLAFYCEDGNVNECEDMQNVIKLTRNNIKAIIMDVMFGGTETVASAIEWALTELMHTPESLKRVQHELANVVGLDRRVEESDFEKLTYFKCVIKEVLRLHPPIPLLLHQSSEDTEVVGYHIPKGTRVMVNGYAVNRDKNAWKDPNTFNPSRFLQSGAPDFKGNNYEFIPFGSGRRSCPGMQLGLYAMEMAVAHLLHSFTWQLPDGIKPSEIDMTDVFGLTAPKAIRLVAVPTPRLLCPLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CZS6</accession>
    <name>A0AAD5CZS6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ATP-dependent (S)-NAD(P)H-hydrate dehydratase</fullName>
            <ecNumber evidence="7">4.2.1.93</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">ATP-dependent NAD(P)HX dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_013993</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ATP, which is converted to ADP. Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(6S)-NADHX + ATP = ADP + phosphate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19017"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:64074"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.93"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>(6S)-NADPHX + ATP = ADP + phosphate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32231"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.93"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the NnrD/CARKD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.93" evidence="7"/>
    <dbReference type="EMBL" id="JAMZMK010006149">
        <property type="protein sequence ID" value="KAI7750495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CZS6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047453">
        <property type="term" value="F:ATP-dependent NAD(P)H-hydrate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110051">
        <property type="term" value="P:metabolite repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046496">
        <property type="term" value="P:nicotinamide nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01171">
        <property type="entry name" value="YXKO-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1190.20:FF:000028">
        <property type="entry name" value="ATP-dependent (S)-NAD(P)H-hydrate dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01965">
        <property type="entry name" value="NADHX_dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000631">
        <property type="entry name" value="CARKD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00196">
        <property type="entry name" value="yjeF_cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12592:SF0">
        <property type="entry name" value="ATP-DEPENDENT (S)-NAD(P)H-HYDRATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12592">
        <property type="entry name" value="ATP-DEPENDENT (S)-NAD(P)H-HYDRATE DEHYDRATASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01256">
        <property type="entry name" value="Carb_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51383">
        <property type="entry name" value="YJEF_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 7" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0521">NADP</keyword>
    <keyword evidence="1 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YjeF C-terminal" evidence="8">
        <location>
            <begin position="49"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="215"/>
            <end position="221"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="255"/>
            <end position="259"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="274"/>
            <end position="283"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="284"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7750495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="357" mass="38510" checksum="994CD600E991CC8B" modified="2024-05-29" version="1">MQQCYGGIIRRQQHLIRCLGGHHHLSNNNNNTRMQSVMSGGGPTLEADAVSILRSISPTLDSSKHKGQAGKIAVLGGCREYTGAPYFSAISALKIGADLSHVICTKDAAPVIKSYSPELIVHPLLEESYSVREEDRKSVSALVLAEVDKWMERFDCLVIGPGLGRDPFLLDCVSDIMKHARQSNVPMVIDGDGLFLVTNRLDLVSGYPLAVLTPNVNEYKRLVQKVLNCEVNDQDGPQQLVSLASSIGGVTILRKGLSDYISDGKEVRSVSIFGSPRRCGGQGDILSGSVAVFLAWARKLVGKEGPTMSPTMLGCIAGSALMRKAASLAFEDKKRSTLTSDIIECLGRSLEDVCPVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D031</accession>
    <name>A0AAD5D031_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cytochrome P450 89A2-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_023321</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006115">
        <property type="protein sequence ID" value="KAI7750615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D031"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016709">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11075">
        <property type="entry name" value="CYP77_89"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051103">
        <property type="entry name" value="Plant_metabolite_P450s"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24298:SF800">
        <property type="entry name" value="CYTOCHROME P450 89A2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24298">
        <property type="entry name" value="FLAVONOID 3'-MONOOXYGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0349">Heme</keyword>
    <keyword evidence="7 8" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="binding site" description="axial binding residue" evidence="7">
        <location>
            <position position="440"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7750615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="500" mass="56409" checksum="81ADFA88EBF2A2F9" modified="2024-05-29" version="1">MCGFLLCRRANKKLLPPGPSFFFSHLLLLTNSLPNLQFILSGLKSKYGPIVTLKIGSHLSVFVGSYSLSYQLLTQKGGTTFSNRPKTWRIGGISSASYGPVWKAYRHNLGSNFLHPSNVKSYSCARKRVLGFMIDRLQKQQEAVGIVKVVDDIELAMLRLSLFMCFGEEVDESRVNEIKSVQDGLLALAGTMRFNVFLVSRWLVKTFFRNTWKKFNHLRIEQKQLLVSLIESRIEGDRQEGGREKMVAYIDTLVKLQVPEEEGTNRNGGKLTQKEMVTLCSEFLVVASETTSSALQWIMANLVKHPSIQQKLYDEIVAVMGPPPPQPGVESKSVLSEETLQKIPYLRAVVLEGLRRHPPAHVALPHSVNEEVELQGYMIPKGATINFMIAEMGWDPEVWDEPLEFKPERFFQNGGSGCVFDIGGSKGIKMMPFGAGRRICPGADLALLHLEYFVANLIWYFEWSVPDGYHVDLKEKVKFTVVMKNPLQTKISSRTKMIMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D3V4</accession>
    <name>A0AAD5D3V4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GDSL esterase/lipase 1-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_012782</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005711">
        <property type="protein sequence ID" value="KAI7752269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D3V4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016298">
        <property type="term" value="F:lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044552">
        <property type="entry name" value="GLIP1-5/GLL25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45966:SF35">
        <property type="entry name" value="GDSL LIPASE_ESTERASE, SGNH HYDROLASE SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45966">
        <property type="entry name" value="GDSL-LIKE LIPASE/ACYLHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7752269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="230" mass="25817" checksum="0CA9B4C2B4075FE8" modified="2024-05-29" version="1" fragment="single">YYSTSSSQTHFVEHVSLFIFGDSLFDPGNNNYINTTPEFQANFWPYGISYFDPPTGRFSDGRLIPDFIAEYAGLPLIPTYLDPSNNEFVYGANFASGGAGALVETHAGFVVDLKTQLEYFGDLEKQLRQDLGDTKTDKLISEAVYLFSCGGNDYLSPVGNNDSVLYPYTHEQYKLKELEEELEGFMYSLFDLNTEVSHRMKNPSKYGNETVHVVVVVLLEAFPAVEARGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D4L6</accession>
    <name>A0AAD5D4L6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_031982</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005258">
        <property type="protein sequence ID" value="KAI7753828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D4L6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7753828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="73" mass="8382" checksum="2FB30BCD10A26785" modified="2024-05-29" version="1" fragment="single">MLPNASCPPISEVRLLLREMSMCGCLYWSRVTDAVNPYCCSTQQIANFEELFLGQEEGNRRDLELNLLIQRNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D500</accession>
    <name>A0AAD5D500_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Histidine kinase/HSP90-like ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_006039</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the heat shock protein 90 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010003865">
        <property type="protein sequence ID" value="KAI7754351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D500"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16927">
        <property type="entry name" value="HATPase_Hsp90-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11260:FF:000005">
        <property type="entry name" value="Heat shock protein 90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.80:FF:000005">
        <property type="entry name" value="heat shock protein 90-5, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000024">
        <property type="entry name" value="heat shock protein 90-5, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.790">
        <property type="entry name" value="Heat shock protein 90, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00505">
        <property type="entry name" value="HSP90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019805">
        <property type="entry name" value="Heat_shock_protein_90_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037196">
        <property type="entry name" value="HSP90_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001404">
        <property type="entry name" value="Hsp90_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020575">
        <property type="entry name" value="Hsp90_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003555">
        <property type="entry name" value="PRK05218.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11528">
        <property type="entry name" value="HEAT SHOCK PROTEIN 90 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13589">
        <property type="entry name" value="HATPase_c_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00183">
        <property type="entry name" value="HSP90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00775">
        <property type="entry name" value="HEATSHOCK90"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110942">
        <property type="entry name" value="HSP90 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00298">
        <property type="entry name" value="HSP90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="Histidine kinase/HSP90-like ATPase" evidence="7">
        <location>
            <begin position="188"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="384"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="387"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7754351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="749" mass="86127" checksum="90C9C0403B43CF66" modified="2024-05-29" version="1" fragment="single">IMESSSRSLPEISPCSDEKVRFLLHTGDRWIAQRTMEARYEGRNIRKYELEVPSDLRWFSELMGEVKRVSGLDYGERSRLSYKSGRVYFCFGVRPGEIGETRYCSSKISMDIHLADLMNHGVKKRTQPNSLLTCISSAVDKRGNSVVVKCEASAVAEKEATDSSAESFEYQAEVSRLMNLIVHSLYSHKEIFLRELVSNASDALDKLRFLSVTDPSLLGDAGELEIRIKPDPEKGTITISDTGIGMTREELIDCLGTIAQSGTSKFLNALKENKDLGSDNSLIGQFGVGFYSAFLVAEKVTVSTKSPKSDKQYIWEASAESSSYVIKEETDPEKQLRRGTEITLYLKDDDKYEFTEPSRVQGLVKNYSQFVSFPIYTWQEKSRTVEVEEEEEPKEGEETPPEGEKKKKKTKTEKYWDWELANETKPIWMRNPKEVEKDQYNEFYKKTFNEFLDPLAYTHFTTEGEVEFRSVIYIPGMAPMNNEDVVNPKTKNIRLYVKRVFISDDFDGELFPRYLSFVKGVVDSNDLPLNVSREILQESRIVRIMRKRLVRKTFDMIQELADSDNKEDYRKFWENFGKFVKLGCIEDTGNHKRITPLLRFFSSKSEDELISLDEYIDNMDEKQNAIYYIATDSLKSAKSAPFLEKLVQKGLEVLYLVEPIDEVAIQNLQTYKEKKFVDVSKEDLELGDTDEVKERENNQEYILLCDWMKQQLGDKVAKVQISSRLSSSPCVLVSGKFGWSANMERLVLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D7E2</accession>
    <name>A0AAD5D7E2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF4057 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_024320</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005580">
        <property type="protein sequence ID" value="KAI7752930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D7E2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025131">
        <property type="entry name" value="DUF4057"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31132">
        <property type="entry name" value="N-LYSINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31132:SF13">
        <property type="entry name" value="N-LYSINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13266">
        <property type="entry name" value="DUF4057"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4057" evidence="2">
        <location>
            <begin position="4"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="122"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="253"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="151"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="258"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7752930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="282" mass="30217" checksum="3ADB3B7BBA9F8E9E" modified="2024-05-29" version="1">MENKTPVRKPNSSTADLLTWPENPNAGSPAVTGSRSSTRSHQPSDGISKVVFGGQVTDEEVEALNKRKPVSGYKLKEITGSGIFAAGGENDSEEADATNRNPSNPTSIRMYQQAVVGISHISFGEDEPVSPKKPISEAKQRELSGTLDSESEARLKKQISDAKNKELSGHNIFAAPPEIQPRPLAARALALRESITIGEPEHNEESVIKTAKKIPNQKLTELSGNNIFTGDEAPQSVEKPLSSAKLREMSGSNIFADGKAEPRDFLRGARKPPGGESSIALV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D804</accession>
    <name>A0AAD5D804_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_015507</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="JAMZMK010000605">
        <property type="protein sequence ID" value="KAI7756068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D804"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009738">
        <property type="term" value="P:abscisic acid-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009651">
        <property type="term" value="P:response to salt stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009414">
        <property type="term" value="P:response to water deprivation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000085">
        <property type="entry name" value="Serine/threonine-protein kinase SRK2E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000045">
        <property type="entry name" value="Serine/threonine-protein kinase SRK2E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24343">
        <property type="entry name" value="SERINE/THREONINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24343:SF546">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE SRK2I-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="20"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7756068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="354" mass="40391" checksum="2FCC072447856B3E" modified="2024-05-29" version="1">MDRSASSAMDLPIMHDSDRYDLVKDIGSGNFGVARLMRDKLTKELVAVKYIERGEKIDENVQREIINHRSLRHPNIVRFRELILTPTHLAIVMEYASGGELFDRICNAGRFNEDEARFFFQQLISGVSYCHSMQVCHRDLKLENTLLDGSPAPRLKICDFGYSKSSVLHSQPKSTVGTPAYIAPEVLLRQEYDGKIADVWSCGVTLYVMLVGGYPFEDPNEPKDFRKTIHRILQVQYSIPENIQISPECRHLISRIFVGDPAQRITMAEIKQHEWFLKNLPANLMDEDKMINSQFEEPDQPMQSVDVIMQIISEATIPPAGLFDLDLMEDDLDDFDSDPDELDIDSSGEIIYAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D8N0</accession>
    <name>A0AAD5D8N0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">60S ribosomal protein L23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_005014</name>
        <name evidence="5" type="ORF">M8C21_025956</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011698">
        <property type="protein sequence ID" value="KAI7726366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAMZMK010002226">
        <property type="protein sequence ID" value="KAI7754957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D8N0"/>
    <dbReference type="SMR" id="A0AAD5D8N0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00337">
        <property type="entry name" value="Ribosomal_uL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.150.20:FF:000003">
        <property type="entry name" value="60S ribosomal protein L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.150.20">
        <property type="entry name" value="Ribosomal protein L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01367">
        <property type="entry name" value="Ribosomal_uL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000218">
        <property type="entry name" value="Ribosomal_uL14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019972">
        <property type="entry name" value="Ribosomal_uL14_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036853">
        <property type="entry name" value="Ribosomal_uL14_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006344">
        <property type="entry name" value="PRK08571.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11761">
        <property type="entry name" value="50S/60S RIBOSOMAL PROTEIN L14/L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11761:SF8">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00238">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01374">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50193">
        <property type="entry name" value="Ribosomal protein L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00049">
        <property type="entry name" value="RIBOSOMAL_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7726366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7754957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="140" mass="15015" checksum="BDD40B074432B368" modified="2024-05-29" version="1">MSKRGRGGSAGNKFRMSLGLPVAATVNCADNTGAKNLYIISVKGIKGRLNRLPSACVGDMVMATVKKGKPDLRKKVMPAVIVRQRKPWRRKDGVFMYFEDNAGVIVNPKGEMKGSAITGPIGKECADLWPRIASAANAIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DAB7</accession>
    <name>A0AAD5DAB7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_024092</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000775">
        <property type="protein sequence ID" value="KAI7755835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DAB7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22162">
        <property type="entry name" value="F-box_AtSKIP3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025886">
        <property type="entry name" value="PP2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32278">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32278:SF118">
        <property type="entry name" value="PHLOEM PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14299">
        <property type="entry name" value="PP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7755835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="319" mass="36023" checksum="6D128C1A35C61B3D" modified="2024-05-29" version="1">MRSLLMLNMCDQDNGFVVDFFGSIPEGFVAKTLSLTSPKDASRLSLVCSVFRSAAEWDAVWESFLPKDYQKIVAQAVEDGGCSLRFGSKKELYLWLCDHPLIIDGGNKSFSLDKQTGKKCYMLAARDLSIVWGDTPRYWRWISLPESRFTEVAELVSVCWFEVHGRISTSMLSPNTLYVAYIVFKSTSETYGFEYQPAEVSIGISGLESQTQTVFLDPEGEPRRRHPVVPRRRMGMYRRGRVAYPIMNQNMIAPVAKSASSNGPRQREDGWMEIELGEYYIKGGEEGELEMSMTEVKGGNWKGGLVIQGIEIRPKPCKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DB14</accession>
    <name>A0AAD5DB14_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Auxin-induced protein X10A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_015939</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ARG7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000603">
        <property type="protein sequence ID" value="KAI7756079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DB14"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009733">
        <property type="term" value="P:response to auxin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003676">
        <property type="entry name" value="SAUR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31929">
        <property type="entry name" value="SAUR-LIKE AUXIN-RESPONSIVE PROTEIN FAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02519">
        <property type="entry name" value="Auxin_inducible"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7756079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="72" mass="8215" checksum="7668E9C9810AC5EE" modified="2024-05-29" version="1">MLDVPKGYIAVYVGEVHKTRFVVPLSFLGYPPFQDLLRQSEEEFGFDHPMGGLTIRCREDAFIDLISRLPVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DBX7</accession>
    <name>A0AAD5DBX7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Fatty acyl-CoA reductase</fullName>
            <ecNumber evidence="1">1.2.1.84</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_021385</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reduction of fatty acyl-CoA to fatty alcohols.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 1">
            <text>a long-chain fatty acyl-CoA + 2 NADPH + 2 H(+) = a long-chain primary fatty alcohol + 2 NADP(+) + CoA</text>
            <dbReference type="Rhea" id="RHEA:52716"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:77396"/>
            <dbReference type="ChEBI" id="CHEBI:83139"/>
            <dbReference type="EC" id="1.2.1.84"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the fatty acyl-CoA reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.84" evidence="1"/>
    <dbReference type="EMBL" id="JAMZMK010000329">
        <property type="protein sequence ID" value="KAI7756634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DBX7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102965">
        <property type="term" value="F:alcohol-forming long-chain fatty acyl-CoA reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080019">
        <property type="term" value="F:alcohol-forming very long-chain fatty acyl-CoA reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035336">
        <property type="term" value="P:long-chain fatty-acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010345">
        <property type="term" value="P:suberin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05236">
        <property type="entry name" value="FAR-N_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026055">
        <property type="entry name" value="FAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013120">
        <property type="entry name" value="FAR_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11011:SF102">
        <property type="entry name" value="FATTY ACYL-COA REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11011">
        <property type="entry name" value="MALE STERILITY PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07993">
        <property type="entry name" value="NAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="1" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioester reductase (TE)" evidence="2">
        <location>
            <begin position="2"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7756634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="284" mass="31426" checksum="BC52F20B5B9A747A" modified="2024-05-29" version="1" fragment="single">FVEKILRVQPNVKKLYLLVRATDAKSALQRFNKEAVAKDLFNILKEKYGSNLQSFLSEKVTPVAGDITYENLGVKDSGLIEEMWGNIDVVVNVAATTNFDERYDIALALNTFGAKNVLNFASQCVKIKLLLHVSTAYVSGETPGLILETPYHMGMALNGAHGLDINTEKKIIEERLKELSYDETSTDKSITLVMKDLGIERANKFGWPNTYVFTKALGEMILGHMKGDMPLVILRPTIITSTYKEPFPGWIEGIRTIDSLAVGYGKGQLSCFLGDPESVIDVVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DCX7</accession>
    <name>A0AAD5DCX7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_008195</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000111">
        <property type="protein sequence ID" value="KAI7757499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DCX7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080188">
        <property type="term" value="P:gene silencing by siRNA-directed DNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044567">
        <property type="entry name" value="CLSY/DRD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45821:SF1">
        <property type="entry name" value="ATP-DEPENDENT HELICASE FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45821">
        <property type="entry name" value="SNF2 DOMAIN-CONTAINING PROTEIN CLASSY 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="7">
        <location>
            <begin position="129"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="8">
        <location>
            <begin position="467"/>
            <end position="635"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7757499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="658" mass="75373" checksum="D4F6350C86C3128C" modified="2024-05-29" version="1">MISDRFREREKGERSMDLSINQHANECEEECDHSFILKEDIGYVCRVCGVVERRIESIIEFQGPKRSKSTKTYCYEGRADKSGESAGAVLDAVKSPGKDFSVEEVAAHPRHKKQMKPHQIEGFNFLLSNLVSDNPGGCILAHAPGSGKTFMLISFIQSFMAKYLDARPLVVLPRGILATWKKEFKRWQVEEIPLFDFYSRKADTRAQQLEVLKQWANTRSILFLGYKQFSTIVCDNDRSAVAVTCQKILLTFPSLVILDEGHTPRNKDTDVFTAVEKVQTPRKVILSGTLYQNHVCEVFNILNLVRPRFLRMEASKTIKRRILSRVSIETRRNLLKKSTDNVFCELVEHALLKDENFKRKVTVIEDLREMTRSVIHYYKGDFLDELPGQVDFSVFLNLSPRQKQEVLEVKKMARKFKVSSDGSAIYVHPELKSLVKSATNGRNDENINKIDALLDNLDARQGVKAKFLLNLLRLCESSDEKLLVFGNYLLPLNFLLKLTMKIKGWRLNKEIFMITGDHDNEERELAMDQFNNSSGAKVFFASIKACGEGISLVGASRIIILDVHLNPSVTRQAIGRAFRPGQVKKVYTYRLVAAGSPEEEDHTTCHRKESIAKMWFEYNEYCGQNEFEMEKINIKDCGDEFLETPWLNEDVTALYKRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DFN1</accession>
    <name>A0AAD5DFN1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_019283</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000053">
        <property type="protein sequence ID" value="KAI7757836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DFN1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7757836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="86" mass="9985" checksum="3BFBD3A92EF94338" modified="2024-05-29" version="1" fragment="single">MTERPDSSRLRLQNFLFMWLIICFGFLSSTKLVKETNLFGSLSETLYTYKTDLFRVSIGNFHSGQQPVEVCLVVSRRKTNKTIECI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G1H0</accession>
    <name>A0AAD5G1H0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_023981</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012205">
        <property type="protein sequence ID" value="KAI7724527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G1H0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="161"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7724527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="275" mass="30825" checksum="800ECA069A3B2197" modified="2024-05-29" version="1">MERLKTRETLEIKGVGFGKGNVKGMSSAMIVPSQDFQQFGRIEFMVEMSKVKKQFDVMVKAKKKIEEALSQIYQKYPEKEEVSTLIAMYEALLEDKPTWSSKATGESMETEAKLKVALARIGEQVNNDFERTDNERMDEGEWNTNEEMIVVAKFVTDLMQPEKSSAGENEAGAEGDEKLVETGTESVVDKDNKAESTMKNMLNESNLLHVAKQTCIRLEEKESVDDKEGLENLVDNNIEEMIEGKDKPMQVKDEPNMDAVEVLMVTDGDGDGSGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G4K0</accession>
    <name>A0AAD5G4K0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_001048</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011193">
        <property type="protein sequence ID" value="KAI7728530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G4K0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="2">
        <location>
            <begin position="19"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7728530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="92" mass="10416" checksum="7DD582EDFE2D950F" modified="2024-05-29" version="1" fragment="single">MKYGKNNSMGFGFLEFDTADTIIHGFTAFEAAKNELHQLFSLFAQSLRIQTSFKKHRGFNFVEDATKQETKNALQALSNTHLYGCHPVGQWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G6A4</accession>
    <name>A0AAD5G6A4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_001584</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010834">
        <property type="protein sequence ID" value="KAI7730179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G6A4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053342">
        <property type="entry name" value="Exosome_cofactor/PTGS_suppr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37260">
        <property type="entry name" value="PHOSPHORELAY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37260:SF2">
        <property type="entry name" value="PROTEIN ECERIFERUM 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="58"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="208"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="287"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="33"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="208"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="287"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="306"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7730179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="316" mass="34451" checksum="48EDC630D071F498" modified="2024-05-29" version="1">MDSKSLAKSKRAHSLHHKKHHPNQKAKGATSSVVPAGAADKAPVKAVVKEKARVGPAALPSNWDRYADEDDPMVESQVDGQTSQPSDVVLPKSKGADYAYLISEAKSQNSTRLSSEFFPSLDEFVSDFGQGKESFFAVRGDSLLSSIQNDSFFVEDKTPANYEASFLSLNMHALANQLTKIERQVYDHHEVTPSTTATKSSTHMMVNSTVHQPGQNSNPNPVASLSANQPKLKAEAAETELDMLLDSFDDVNLKEKASSTLTSKKATLDFDIDGTLDDLLKETSTPSLLDQSNASQTQHLSKPEPIDDFDSWLDTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G6W1</accession>
    <name>A0AAD5G6W1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Core Histone H2A/H2B/H3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_012379</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the histone H2B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010931">
        <property type="protein sequence ID" value="KAI7729701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G6W1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22910">
        <property type="entry name" value="HFD_H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000043">
        <property type="entry name" value="Histone H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000558">
        <property type="entry name" value="Histone_H2B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23428">
        <property type="entry name" value="HISTONE H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00621">
        <property type="entry name" value="HISTONEH2B"/>
    </dbReference>
    <dbReference type="SMART" id="SM00427">
        <property type="entry name" value="H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="4">
        <location>
            <begin position="115"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="29"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="29"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="56"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7729701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="232" mass="25530" checksum="9DB8677B1173AF47" modified="2024-05-29" version="1">MAPKKKTVVKTTRKTIRETVQVSVIDQTTQTPITNNSSSNEKVEIFTIQTPQQQETLEDDDETQMDDEDEENDVVTGQKTREIEVQDATPTPTPKKEQKGKKTTTREGVVVAGGEKRKKKRRKLGESGGEGYKRYVHKVLKQVHPDLGISSKAMTIINNLVGDMFERLAEVAARLAMYNKKMTLTSREIQAAVKLVLPGELGKHAIAEGTKAVTNYVSYGGRGGGGGGRTRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G7S5</accession>
    <name>A0AAD5G7S5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_021470</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small hydrophilic plant seed protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010726">
        <property type="protein sequence ID" value="KAI7730586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G7S5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009737">
        <property type="term" value="P:response to abscisic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038956">
        <property type="entry name" value="LEA_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022377">
        <property type="entry name" value="Sm_Hydphi_plant_seed_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000389">
        <property type="entry name" value="Small_hydrophilic_seed_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34671">
        <property type="entry name" value="EM-LIKE PROTEIN GEA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34671:SF11">
        <property type="entry name" value="EM-LIKE PROTEIN GEA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00477">
        <property type="entry name" value="LEA_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00431">
        <property type="entry name" value="SMALL_HYDR_PLANT_SEED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="32"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="107"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7730586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="152" mass="16517" checksum="4A43E67FCDB09714" modified="2024-05-29" version="1">MAQAQEKRSELDAKAQHGETVVPGGTGGKSLEAQEHLAEGRSKGGQTRKEQLGTEGYQEMGSKGGQVRKEQLGTEGYQELGSKGGQARKEQLGTEGYQEMGSKGGQTRKEQMESEGYKEMGRKGGLTTTDKSAQERVEEEGIEIDESKYRTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G7Z9</accession>
    <name>A0AAD5G7Z9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Proliferating cell nuclear antigen</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_015976</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PCNA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010695">
        <property type="protein sequence ID" value="KAI7730633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G7Z9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043626">
        <property type="term" value="C:PCNA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030337">
        <property type="term" value="F:DNA polymerase processivity factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006272">
        <property type="term" value="P:leading strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006275">
        <property type="term" value="P:regulation of DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019985">
        <property type="term" value="P:translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00577">
        <property type="entry name" value="PCNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.70.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046938">
        <property type="entry name" value="DNA_clamp_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000730">
        <property type="entry name" value="Pr_cel_nuc_antig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022649">
        <property type="entry name" value="Pr_cel_nuc_antig_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022648">
        <property type="entry name" value="Pr_cel_nuc_antig_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11352">
        <property type="entry name" value="PROLIFERATING CELL NUCLEAR ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11352:SF8">
        <property type="entry name" value="PROLIFERATING CELL NUCLEAR ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02747">
        <property type="entry name" value="PCNA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00705">
        <property type="entry name" value="PCNA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55979">
        <property type="entry name" value="DNA clamp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proliferating cell nuclear antigen PCNA N-terminal" evidence="3">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="Proliferating cell nuclear antigen PCNA C-terminal" evidence="4">
        <location>
            <begin position="78"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7730633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="181" mass="20193" checksum="794771A9E8B3EBA0" modified="2024-05-29" version="1" fragment="single">HYRCVHIVSLGMNLGTVAKMLKCAGNDDIITIKANKGDDCVNFMFECPRKDSFLSYLFALTKLMNVDYEPLHIPEIRYDAIFYTGTANIVCRQNTIGDKTEEATVIEMVKPVSAKFGLSYLNSFTYATPLASQVTLSLCSKGPLMVEYKIAEMGHLRFYLAPKIKEEKKINGSSKEGGTII</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G8K0</accession>
    <name>A0AAD5G8K0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">LanC-like protein GCL1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_012518</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LanC-like protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010213">
        <property type="protein sequence ID" value="KAI7732462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G8K0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031179">
        <property type="term" value="P:peptide modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04794">
        <property type="entry name" value="euk_LANCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007822">
        <property type="entry name" value="LANC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020464">
        <property type="entry name" value="LanC-like_prot_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12736">
        <property type="entry name" value="LANC-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12736:SF25">
        <property type="entry name" value="LANC-LIKE PROTEIN GCL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05147">
        <property type="entry name" value="LANC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01951">
        <property type="entry name" value="LANCEUKARYTE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01950">
        <property type="entry name" value="LANCSUPER"/>
    </dbReference>
    <dbReference type="SMART" id="SM01260">
        <property type="entry name" value="LANC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158745">
        <property type="entry name" value="LanC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="7"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7732462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="430" mass="46419" checksum="01FCB059E4ACF2BB" modified="2024-05-29" version="1">MSSMLKLTSSTDNNNNNNSSHDDVHLDFLNNPTVDAAADNISPSPETFLEAAIALKEQVVKATWTDVCAGGVEIIDPTVYTGLLGTAFTCLRFHEATGSHQDLQLSADIVEACANVVTNTSTRYMTFLCGRGGIYALGAVIANYLGDHRKRDFYLAEFMELAQDTALPAGPEEGGFGMSYDLLHGRAGFLWAALFINKHIGQETVPSDVLNPIVKAVIAGGRAGAPDHAMCPLMYRWHGTRYWGAAHGLAGILHVLLHFPLCEEDADDVKGTLKYMMSKRFPHTGNYPSSEGNARDNLVQWSHGAGGMAITLCKASQVFPHDRELRDAAIEAGEVVWKSGLVEKAGLADGASGNAYAFLALYRLTGDSIYVDRAKAFGGFLFANARKLMTSTTSHGLDHGFSLFEGLAGAACLWSDMVRPDESRFPGFEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G9B0</accession>
    <name>A0AAD5G9B0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Macro domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_025326</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010301">
        <property type="protein sequence ID" value="KAI7732131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G9B0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02908">
        <property type="entry name" value="Macro_OAADPr_deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.220.10">
        <property type="entry name" value="Leucine Aminopeptidase, subunit E, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002589">
        <property type="entry name" value="Macro_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043472">
        <property type="entry name" value="Macro_dom-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001664">
        <property type="entry name" value="PRK00431.1-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11106">
        <property type="entry name" value="GANGLIOSIDE INDUCED DIFFERENTIATION ASSOCIATED PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11106:SF27">
        <property type="entry name" value="MACRO DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01661">
        <property type="entry name" value="Macro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00506">
        <property type="entry name" value="A1pp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52949">
        <property type="entry name" value="Macro domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51154">
        <property type="entry name" value="MACRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Macro" evidence="1">
        <location>
            <begin position="43"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7732131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="230" mass="24888" checksum="80D3E9AA6673ED79" modified="2024-05-29" version="1">MPLISSPSLYCSLKSPNYMLSTFVNPGSYSLRQRTRASSNMSTNVAQFNLSPTSFLKILKGDITQWSIDGSSDAIVNPANERMLGGGGADGAIHRAAGPELKTTCYDVPEVRPGVRCPTGEARITPGFKLPASHVIHTVGPVYDVDSNAPASLSNAYRNSLRVARENNIQYVAFPAISCGVYGYPFDEAATVAISTIKEHCDAIKEVHFVLFSDDIYNVWLKKAGELLPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GBZ3</accession>
    <name>A0AAD5GBZ3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Patatin</fullName>
            <ecNumber evidence="7">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_011847</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Lipolytic acyl hydrolase (LAH).</text>
    </comment>
    <comment type="domain">
        <text evidence="7">The nitrogen atoms of the two glycine residues in the GGXR motif define the oxyanion hole, and stabilize the oxyanion that forms during the nucleophilic attack by the catalytic serine during substrate cleavage.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the patatin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="7"/>
    <dbReference type="EMBL" id="JAMZMK010009272">
        <property type="protein sequence ID" value="KAI7736452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GBZ3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047372">
        <property type="term" value="F:monoacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004620">
        <property type="term" value="F:phospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07214">
        <property type="entry name" value="Pat17_isozyme_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1090.10:FF:000005">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32176:SF80">
        <property type="entry name" value="ACYL TRANSFERASE_ACYL HYDROLASE_LYSOPHOSPHOLIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32176">
        <property type="entry name" value="XYLOSE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 6 7" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="5 6 7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="3" id="KW-0611">Plant defense</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PNPLA" evidence="9">
        <location>
            <begin position="23"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="376"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXGXXG" evidence="6">
        <location>
            <begin position="27"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="6">
        <location>
            <begin position="65"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DGA/G" evidence="6">
        <location>
            <begin position="232"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="387"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="67"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7736452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="396" mass="43452" checksum="7D63ACCEB54E22A2" modified="2024-05-29" version="1">MSYMEGPRSPLQPPTYGNLITILSIDGGGVRGIIPGVILDFLETELQKLDGENARLADYFDVIAGTSTGGIVTAMLTTPNEEGRPVFTAKDVVDFYVEHCPKIFPHDSSSDLHSEHRHRHLFGKVVDPSKKAIKALMGPKHNGRHLHNILQEKLQGRRLHEAVTNVVIPTFDIKYLQPVIFSSYQLKTTPSLNAKFSDICIGTSAAPTYLPSHSFETKDSEGKVLREFNLIDGAVVANNPTLVAISEITKEITKGSADFFPIKPMDYGRLLVLSIGTGTPKFQEKYDATQSSSWGVLGWLAGGGSTPLVDVFTQASGDMVDYHVSTVFQALHSTENYLRIQDDTLSGELATLDLATQENLENLVKVGERLLKKQVSRVSHNSSPGEECPRIKIAEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GC79</accession>
    <name>A0AAD5GC79_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Calcineurin B-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_005469</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Acts as a calcium sensor. CBL proteins interact with CIPK serine-threonine protein kinases. Binding of a CBL protein to the regulatory NAF domain of a CIPK protein lead to the activation of the kinase in a calcium-dependent manner.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer. Interacts with CIPK.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the calcineurin regulatory subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009642">
        <property type="protein sequence ID" value="KAI7734803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GC79"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019900">
        <property type="term" value="F:kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000073">
        <property type="entry name" value="calcineurin B-like protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045198">
        <property type="entry name" value="CNBL1-10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23056">
        <property type="entry name" value="CALCINEURIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23056:SF138">
        <property type="entry name" value="CALCINEURIN B-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13202">
        <property type="entry name" value="EF-hand_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="40"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="77"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU369080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7734803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="132" mass="15086" checksum="F874E4682382B1B2" modified="2024-05-29" version="1" fragment="single">SVSEIEALYELFKKISSAVIDDGLINKEEFQLALFKTNKKESLFADRVFDLFDTKHNGILGFEEFARALSVFHPNAPIDDKIEFSFQLYDLKQQGFIERQEVKQMVVATLAESGMNLSDDVIESIIDKVDFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GE19</accession>
    <name>A0AAD5GE19_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CID domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_001971</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009207">
        <property type="protein sequence ID" value="KAI7736723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GE19"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16981">
        <property type="entry name" value="CID_RPRD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000018">
        <property type="entry name" value="ENTH/VHS family protein isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006569">
        <property type="entry name" value="CID_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12460:SF0">
        <property type="entry name" value="CID DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12460">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE INHIBITOR-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04818">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00582">
        <property type="entry name" value="RPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51391">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CID" evidence="4">
        <location>
            <begin position="3"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="126"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="254"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="135"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7736723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="293" mass="32819" checksum="6BD4C0C410C73D50" modified="2024-05-29" version="1">MSDDVFDGQALSDKLFKLNSSQQSIESLSKSCISQRKKAKQIVEIWHKSFKTAQREQHVTFLYLANDILQNSRRKGSEFVNEFWKVMPSALKFVNEHGDDNGKKAASRLVGIWDERRVFGSRGQNLKDEMLGKSPPTQLASNGKTSNTNPIKIVKRDANSLRLVSTISCFISDLLGYVLTSFDCSVPNFVCVLDFQKLAVGGLPEKIMTAFHLVHDENATEDTALNNCESAVRRFGDLERDSESQGVVPGSSLLDEIQEQENVLQDCVNQLETAERTRTSLISQLKEAIQDQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GF75</accession>
    <name>A0AAD5GF75_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_005042</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008870">
        <property type="protein sequence ID" value="KAI7738146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GF75"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7738146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="250" mass="28390" checksum="48BD9110FB0B1440" modified="2024-05-29" version="1">MIQKTSMFGLRFKGTGNGTIPDYRDFAADGHVWGREQGYLNPQGHMVFCKTLEETRPVQSRLCMFTSDLGLRIRMGSRMVRVKHHQGRKLEYFIKVSNGSLLVGSLERHRIAAERNISLRDIGVRWDADESLITGWKQAPNSAAELLELQQLSFLLQQEGSSDGSFSVASYKEMAVKDKGLHRDSLMRWIEWIPIKKTYLGQILIKGFKALRLFKHIREMKIKVVGELIGGGQKGRTSWLLGIPILVESS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GIP1</accession>
    <name>A0AAD5GIP1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA-directed RNA polymerase subunit beta</fullName>
            <ecNumber evidence="8">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_023848</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the RNA polymerase beta chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="8"/>
    <dbReference type="EMBL" id="JAMZMK010008234">
        <property type="protein sequence ID" value="KAI7741238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GIP1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032549">
        <property type="term" value="F:ribonucleoside binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00653">
        <property type="entry name" value="RNA_pol_B_RPB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.150:FF:000005">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1800.10:FF:000006">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.270.10">
        <property type="entry name" value="DNA-directed RNA polymerase, subunit 2, domain 6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1800.10">
        <property type="entry name" value="RNA polymerase alpha subunit dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015712">
        <property type="entry name" value="DNA-dir_RNA_pol_su2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007120">
        <property type="entry name" value="DNA-dir_RNAP_su2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037033">
        <property type="entry name" value="DNA-dir_RNAP_su2_hyb_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007121">
        <property type="entry name" value="RNA_pol_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007646">
        <property type="entry name" value="RNA_pol_Rpb2_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007647">
        <property type="entry name" value="RNA_pol_Rpb2_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007641">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014724">
        <property type="entry name" value="RNA_pol_RPB2_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20856">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04566">
        <property type="entry name" value="RNA_pol_Rpb2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04567">
        <property type="entry name" value="RNA_pol_Rpb2_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00562">
        <property type="entry name" value="RNA_pol_Rpb2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04560">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01166">
        <property type="entry name" value="RNA_POL_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 8" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="11">
        <location>
            <begin position="10"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="12">
        <location>
            <begin position="54"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed RNA polymerase subunit 2 hybrid-binding" evidence="9">
        <location>
            <begin position="110"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="10">
        <location>
            <begin position="461"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="562"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF04566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF04567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7741238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="562" mass="62600" checksum="8CEE0B34E8FF544D" modified="2024-05-29" version="1" fragment="single">ITQIESTGSLVEIKRDAKHKEVRIFCGAGRLLRPLLVVKNLHKIKLLKGGSYSFQDLLDNGVVELIGTEEEEDCSTAWDIKYLFLENGDHNPAKYTHCELDMSFMLGLSCGIIPFANHDHAKRVLFQSQKHSQQAIGHSTTNPDIRADTLSHQLFYPQRPLFKTVLSDCLEKGQPFGQSQKRMIPRPEFYNGQCAIVAVNVHLGYNQEDSLVMNRACLDRGMFRSEHTRSYKAEVENNEAVGKKPKSDEAVNFGKIQSKIGRVDSLDDDGFPFVGANLQSGDIVIGKFTEAGTDHSVKLKHTERGMVEKVVLSANDEGKNFAVVSLRQVRSPCLGDKFSSMHGQKGVLGYLESQENFPFTVQGMVPDIVINPHAFPSRQTPGQMLEAALGKGIALGGSQRYATPFSSLSVDAITDQLHRAGYSRWGNERIYDGRTDKVKFRNTGPVHPLTRQPVADRKRFGGIKFGEMERDCLIAHGASANLHERLFTLSDCSEMHVCRKCKRMASVIQRSVSGGHKIRGPYCRICDSMEDVIKVSVPYGAKLLSQELFSMGISLKYDTELC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GJK5</accession>
    <name>A0AAD5GJK5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein TIFY</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Jasmonate ZIM domain-containing protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_029928</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Repressor of jasmonate responses.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="2">The jas domain is required for interaction with COI1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the TIFY/JAZ family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007322">
        <property type="protein sequence ID" value="KAI7745240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GJK5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031347">
        <property type="term" value="P:regulation of defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000022">
        <property type="term" value="P:regulation of jasmonic acid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009611">
        <property type="term" value="P:response to wounding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018467">
        <property type="entry name" value="CCT_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040390">
        <property type="entry name" value="TIFY/JAZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010399">
        <property type="entry name" value="Tify_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33077">
        <property type="entry name" value="PROTEIN TIFY 4A-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33077:SF90">
        <property type="entry name" value="PROTEIN TIFY 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09425">
        <property type="entry name" value="Jas_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06200">
        <property type="entry name" value="tify"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00979">
        <property type="entry name" value="TIFY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51320">
        <property type="entry name" value="TIFY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1184">Jasmonic acid signaling pathway</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tify" evidence="4">
        <location>
            <begin position="49"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="159"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="161"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU369065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7745240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="181" mass="19815" checksum="EEA2431F1BC82524" modified="2024-05-29" version="1">MERDFMGLNSKEEAIESVLLKSSAAQWSLSNGPEDLNNRQSAENQKNIDLNGPTQLTIFYRGMVNVYDDMPPEKAHAIMSLARNEARAQAHAATSRTPAVDVVHVGQPMTTSSPVSVGPSTARDAPKAMSSFRRVMQSDVPQMRGASLARFLEKRRERVMASAPYNSKPASSSAPSKDEHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GKN5</accession>
    <name>A0AAD5GKN5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_004909</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007520">
        <property type="protein sequence ID" value="KAI7744504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GKN5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042179411" evidence="1">
        <location>
            <begin position="21"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7744504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="220" mass="25449" checksum="EBFCBFBEB874DDA3" modified="2024-05-29" version="1" fragment="single">QPYLLIIAFFSILLHRRLQSLHLHIPSNSTLALSGFAPLEKQHANNYVHGVICQLEHSYQDEYHVTQCRSDMPLVLDRAREIYDSELEKKTKYDGDVKKPFNFRVEDGGFNLNESQMCVDKIACERPGFPKDVKFEFLSKFGGMGRMKRLYEMLKSVNARSWESDAIVSIRRKSTNVKLSDRAPSPDVKLNLLMEIAEEYELDWDPTASETKLLKPHEDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GLH8</accession>
    <name>A0AAD5GLH8_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_002966</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006797">
        <property type="protein sequence ID" value="KAI7747295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GLH8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006927">
        <property type="entry name" value="DUF639"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31860:SF6">
        <property type="entry name" value="HEAT-INDUCIBLE TRANSCRIPTION REPRESSOR (DUF639)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31860">
        <property type="entry name" value="HEAT-INDUCIBLE TRANSCRIPTION REPRESSOR (DUF639)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04842">
        <property type="entry name" value="DUF639"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="184"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="268"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7747295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="338" mass="39003" checksum="B1AC89209AACFB99" modified="2024-05-29" version="1">MAAVSKTRNMIEGFVRDGSFKWLLKNRNPFHEEFEEMKASPSAGKNWIHELSPVANIVVRRCSKILKVPTGQLQENFNAEAIDAIKDPSCYARNFLEYSCYKALSAYTQVSGYMDDRKFRRLTFDMMLAWEVPDAASQPTNTMDELVFPVTELGNHLVKLFYWEEPIKSSIFCLVSTYVIYRGWFSYVVGLVLAFIALFIVITRWFSRGREVDEFRVIAPPPMNTMEQLLAVQNAISQAEELIQDGNILLLKLRGLLLSIYPQASDRFAIALVIMAVFVAVVPMKYLILAVLLEEWTKYSPMRRSSTERLNRRLREWWFSIPAAPVVLEKVNDDKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GLW1</accession>
    <name>A0AAD5GLW1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Vesicle transport protein GOT1B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_013826</name>
        <name evidence="8" type="ORF">M8C21_013930</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the GOT1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008613">
        <property type="protein sequence ID" value="KAI7739521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAMZMK010007544">
        <property type="protein sequence ID" value="KAI7744373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GLW1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045176">
        <property type="entry name" value="Got1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007305">
        <property type="entry name" value="Vesicle_transpt_Got1/SFT2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21493">
        <property type="entry name" value="CGI-141-RELATED/LIPASE CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21493:SF9">
        <property type="entry name" value="GOLGI TRANSPORT PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04178">
        <property type="entry name" value="Got1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="55"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7739521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7744373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="102" mass="11751" checksum="03C939855EAE870B" modified="2024-05-29" version="1">MNQILFISGVIMTIGVKSSLQFFMRRNNFKGTISFGIGFFFVIIGWPVIGMLAETYGFIILFSGFWPTLSVFVQKIPIIGWVFQQPFIRSFLDRYRGKRAPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GNN9</accession>
    <name>A0AAD5GNN9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">M8C21_018128</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">AA19_3_7</strain>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010006879">
        <property type="protein sequence ID" value="KAI7746906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GNN9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000239">
        <property type="entry name" value="Proline-rich receptor-like protein kinase PERK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000207">
        <property type="entry name" value="proline-rich receptor-like protein kinase PERK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047117">
        <property type="entry name" value="PERK1-13-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47982">
        <property type="entry name" value="PROLINE-RICH RECEPTOR-LIKE PROTEIN KINASE PERK4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47982:SF6">
        <property type="entry name" value="PROLINE-RICH RECEPTOR-LIKE PROTEIN KINASE PERK4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 16 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 16 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 17" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="90"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAI7746906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="425" mass="46890" checksum="F30D8FE85813572D" modified="2024-05-29" version="1">MCKKKKKKGYYIPEQSAGVGGGGPYYYGKPDHVVRVGTGGGWGQPPQPPPVNSSSEFPGPGMSMGSGNVPLAFSQSQFSYDELVAATGGFTQYNLLGQGGFGFVHKGVLPSGQEVAIKSLKPGSGQGEREFQAEVEIISRVHHRYLVSLVGYCISDGQRMLVYEYVPNNTLEFHLHGKGQVVMDWQTRMRIALGSAKGLAYLHEDCHPRIIHRDIKSANILLDHDYEAKVADFGLAKLTTANDTHVSTRVMGTFGYLAPEYASSGKLSDKSDVFSFGVMLLEILTGRKPIDPETEYMNDSLVEWARPLIAKAKEDGDYNELVDPRLKANYDRNEMEQMASCAAASIRHSARRRPKMSQIVRALEGNALLDSLDEKEIREEAALVTSESNVYDTRAYNDDMLRFRKMIMSTEESSGSEMGSSGPYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GRW0</accession>
    <name>A0AAD5GRW0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Sm-like protein LSM2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_008455</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of LSM protein complexes, which are involved in RNA processing.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the snRNP Sm proteins family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006274">
        <property type="protein sequence ID" value="KAI7749803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GRW0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990726">
        <property type="term" value="C:Lsm1-7-Pat1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005688">
        <property type="term" value="C:U6 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01725">
        <property type="entry name" value="LSm2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000009">
        <property type="entry name" value="U6 snRNA-associated Sm-like protein LSm2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016654">
        <property type="entry name" value="U6_snRNA_Lsm2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13829">
        <property type="entry name" value="SNRNP CORE PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13829:SF2">
        <property type="entry name" value="U6 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016394">
        <property type="entry name" value="U6_snRNA_Lsm2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 9" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 9" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Sm" evidence="10">
        <location>
            <begin position="2"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR016394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7749803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="93" mass="10719" checksum="CEBB503675E3119F" modified="2024-05-29" version="1">MLFFSYFKDLVGREVTVELKNDLAIRGTLHSVDQYLNIKLENTRVVDQDKFPHMLSVRNCFIRGSVVRYVQLPPDGVDIDLLHDATRREARGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GTL7</accession>
    <name>A0AAD5GTL7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Receptor-like serine/threonine-protein kinase</fullName>
            <ecNumber evidence="19">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="26" type="ORF">M8C21_021688</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="26">AA19_3_7</strain>
            <tissue evidence="26">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the leguminous lectin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="26">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="19"/>
    <dbReference type="EMBL" id="JAMZMK010002035">
        <property type="protein sequence ID" value="KAI7755037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GTL7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002229">
        <property type="term" value="P:defense response to oomycetes"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048544">
        <property type="term" value="P:recognition of pollen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00028">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01098">
        <property type="entry name" value="PAN_AP_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000195">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000240">
        <property type="entry name" value="Lectin-domain containing receptor kinase A4.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.90.10.10">
        <property type="entry name" value="Bulb-type lectin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001480">
        <property type="entry name" value="Bulb-type_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036426">
        <property type="entry name" value="Bulb-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000858">
        <property type="entry name" value="S_locus_glycoprot_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024171">
        <property type="entry name" value="SRK-like_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32444">
        <property type="entry name" value="BULB-TYPE LECTIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32444:SF89">
        <property type="entry name" value="S GLYCOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01453">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08276">
        <property type="entry name" value="PAN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00954">
        <property type="entry name" value="S_locus_glycop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000641">
        <property type="entry name" value="SRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00108">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00473">
        <property type="entry name" value="PAN_AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51110">
        <property type="entry name" value="alpha-D-mannose-specific plant lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50927">
        <property type="entry name" value="BULB_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50948">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 19 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 19" id="KW-0418">Kinase</keyword>
    <keyword evidence="13 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 19 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-0675">Receptor</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 19" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8 22" id="KW-0732">Signal</keyword>
    <keyword evidence="6 19" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="22">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042139655" description="Receptor-like serine/threonine-protein kinase" evidence="22">
        <location>
            <begin position="23"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="436"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="domain" description="Bulb-type lectin" evidence="24">
        <location>
            <begin position="23"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="25">
        <location>
            <begin position="338"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="508"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="536"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRNR" id="PIRNR000641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="EMBL" id="KAI7755037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="727" mass="82133" checksum="3B4A386811AADE9D" modified="2024-05-29" version="1">MSTILPLILCSLLFLNLQTSISTDTLKPTESFVDGFTLISSNQRFEFGFFSLGNSSSRDLYLGIWYYNLPFTVVWLANRNNPIRDSFGEISLKENGTLFLYSRSKGIVWSTDNITITSSFTPVLQLLDSGNLVIRDDGGNKTIFWESFDFPTDTLLPDLKLGSKLYVGLHRFLRSWKSNEDPSEGAYSFGLEPPEAPQLVLRSGSRKLSRWGPFDGTLFSGGTSLTSSPLFQNFYASNSEEVYFKYELLDESILSRLVISPTGLLQYFTWRVNSSKEWNLLLTFNSDPCDNYAKCGPYGSCYSLSSCKCLSGFMPDSPRDWGLFSYSGGCRRKHELSCGHGDGFVKYNALKFPDNPVVWGNYTNHDCEAKCLKNCSCMAYANVNVYGNGSRCVGWIGDLVDLKYLPNGGEQIYIRMAHTEIKSIADAKRKRSTVKIVSVVTVIVVLLLSGGAIWYLSWIIRHRRTDQIGRSSKAQQSGSFQYIDDNQDSGCQLPLYDLETICLACNNFSERNKIGQGGFGSVYKGELANGQEIAVKRLVEKSLQGVEELKNEIILIAKLQHRNLVKLLGCCIEGEETMLVYEYLPNKSLNNFIYDKPTRKLLTWRKRYHIIKGIARGLLYLHQDSRLRIIHRDLKTSNILLDSELNPKISDFGIARIFGTDQIQDMTKRIIGTYGYMSPEYAMNGHYSVKSDVYSFGVIVLEIISGQKNWAFHHPDHDLNLLGHTIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GTR4</accession>
    <name>A0AAD5GTR4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TLC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_025664</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010001583">
        <property type="protein sequence ID" value="KAI7755217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GTR4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006634">
        <property type="entry name" value="TLC-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050846">
        <property type="entry name" value="TLCD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13439">
        <property type="entry name" value="CT120 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13439:SF0">
        <property type="entry name" value="TOPOISOMERASE I DAMAGE AFFECTED PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03798">
        <property type="entry name" value="TRAM_LAG1_CLN8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00724">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50922">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="108"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="157"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="190"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="228"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="TLC" evidence="7">
        <location>
            <begin position="56"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7755217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="267" mass="30256" checksum="AC2D0C919AD9B98D" modified="2024-05-29" version="1" fragment="single">MAPSGFSTIVDHASSSNPRLWLFSVFGGILMCKIVYELTGIISPLIYKGFVKLENAQKLEWKNRGFSTFHAIFVAIGSVYLLVISDLFDEHARDEWIINRSSALSDAVLGISIGYFLTDLGMIIWTYPTLGGFEYFLHHGLSLLAIGQSLLSGQVQFYILIVLFTEITTPFVNLRWYLDVSGKKNSTLYMLNGVALFVGWLAARVILFVFFFSHMYARLYQVKQVYTMGFYSTVTIPPALALMNLFWFWKIAKGLIKTLTKAKGHKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GUC7</accession>
    <name>A0AAD5GUC7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Xyloglucan endotransglucosylase/hydrolase</fullName>
            <ecNumber evidence="8">2.4.1.207</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_027554</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Secreted</location>
            <location evidence="8">Cell wall</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Secreted</location>
            <location evidence="8">Extracellular space</location>
            <location evidence="8">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="8">Contains at least one intrachain disulfide bond essential for its enzymatic activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the glycosyl hydrolase 16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.207" evidence="8"/>
    <dbReference type="EMBL" id="JAMZMK010005640">
        <property type="protein sequence ID" value="KAI7752666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GUC7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016762">
        <property type="term" value="F:xyloglucan:xyloglucosyl transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042546">
        <property type="term" value="P:cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010411">
        <property type="term" value="P:xyloglucan metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02176">
        <property type="entry name" value="GH16_XET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000025">
        <property type="entry name" value="Xyloglucan endotransglucosylase/hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000757">
        <property type="entry name" value="Beta-glucanase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044791">
        <property type="entry name" value="Beta-glucanase/XTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008264">
        <property type="entry name" value="Beta_glucanase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010713">
        <property type="entry name" value="XET_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016455">
        <property type="entry name" value="XTH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31062">
        <property type="entry name" value="XYLOGLUCAN ENDOTRANSGLUCOSYLASE/HYDROLASE PROTEIN 8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00722">
        <property type="entry name" value="Glyco_hydro_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06955">
        <property type="entry name" value="XET_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005604">
        <property type="entry name" value="XET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00737">
        <property type="entry name" value="GLHYDRLASE16"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51762">
        <property type="entry name" value="GH16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0052">Apoplast</keyword>
    <keyword evidence="8" id="KW-0134">Cell wall</keyword>
    <keyword evidence="8" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 8" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="1 8" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041780511" description="Xyloglucan endotransglucosylase/hydrolase" evidence="8">
        <location>
            <begin position="27"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="GH16" evidence="9">
        <location>
            <begin position="21"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="102"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="106"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="106"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="6">
        <location>
            <position position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR005604-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR005604-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR608264-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7752666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="293" mass="33230" checksum="C15BFD73CB23F9FD" modified="2024-05-29" version="1">MAISSSSSSFFVIALFLSLLVISANATAKFEELFHPYWAADHFSFDGDAVNMKLDNFSGAGFSSKSKYMFGKVNVQIKLVEGDSAGTVTAFYMSSDGPKHHEFDFEFLGNTTGEPYIVQTNVYVNGVGNREQRLNLWFDPTKDFHTYSLLWNQRQVVFLVDETPIRVHTNLEHKGIPFPRDQAMGVYSSIWNADDWATQGGRVKTDWTHAPFVASYRAFEIDGCECPVTTTQSDNAKRCANSGGWWDQPVMSELNLHQSHQLIWVRANHMIYDYCSDTGRFPSVPVECEHHRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GUS1</accession>
    <name>A0AAD5GUS1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Gnk2-homologous domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_015549</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010004938">
        <property type="protein sequence ID" value="KAI7753984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GUS1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23509">
        <property type="entry name" value="Gnk2-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.430.20:FF:000015">
        <property type="entry name" value="Cysteine-rich receptor-like protein kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.430.20">
        <property type="entry name" value="Gnk2 domain, C-X8-C-X2-C motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052059">
        <property type="entry name" value="CR_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002902">
        <property type="entry name" value="GNK2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038408">
        <property type="entry name" value="GNK2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47973">
        <property type="entry name" value="CYSTEINE-RICH RECEPTOR-LIKE PROTEIN KINASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01657">
        <property type="entry name" value="Stress-antifung"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51473">
        <property type="entry name" value="GNK2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="255"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="Gnk2-homologous" evidence="11">
        <location>
            <begin position="26"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="Gnk2-homologous" evidence="11">
        <location>
            <begin position="133"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="348"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7753984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="368" mass="41581" checksum="44E1A3B5B530BDB1" modified="2024-05-29" version="1">MEIALICRQRHKIFHVQFFAKYHILEDEEIHTYNISKSNGDARTERIKLHCDDTLEDDREIFLQNYIPVAGTGPNANYGLAQCYNDLSSQDCILCYAQIRTYLPRCYGGNGGWIYLDGCFARVQNYSFFEEYTSPNDATVCGNTTSKSILFQESTSRAVINVVSGALRNNDYFAREEMLVSGSNDSVYVLAECWKTLSPSSCSACLENASMSITKCLPWSEGRVLNTGCFMRYSDTNFLNLIPVTESSSNKARKIAIAVSVVSTVVVITVALMTVLYIRKRRYIEHRRKGSYDVDKLAKILNDSSLNFNYSTVEKATGHWNDSNKLGQGGFGTVYKGVLADGREIAVKRLFCNYKFKAADFFNELFRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GV59</accession>
    <name>A0AAD5GV59_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_000082</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily. AB hydrolase 4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005584">
        <property type="protein sequence ID" value="KAI7752923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GV59"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047372">
        <property type="term" value="F:monoacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034338">
        <property type="term" value="F:short-chain carboxylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050960">
        <property type="entry name" value="AB_hydrolase_4_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10794">
        <property type="entry name" value="ABHYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10794:SF95">
        <property type="entry name" value="ALCOHOL O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7752923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="136" mass="15155" checksum="3DA7FDD96902A1DD" modified="2024-05-29" version="1">MLSTHIFNYLSDISLQACSVGEFNKYTGRITGNFETVDTFHRWASSVHVVNNVKVPLVCINAIDDPVCTHEAIPFDECRMNKNIVLATTQHGGHNAFFEGMSGKNLWWVGVIEEYFSVLHSSSLMNKNTGAPAFNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GW76</accession>
    <name>A0AAD5GW76_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Carboxypeptidase</fullName>
            <ecNumber evidence="11">3.4.16.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_023893</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Probable carboxypeptidase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the peptidase S10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.-" evidence="11"/>
    <dbReference type="EMBL" id="JAMZMK010003090">
        <property type="protein sequence ID" value="KAI7754511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GW76"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000060">
        <property type="entry name" value="Carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033124">
        <property type="entry name" value="Ser_caboxypep_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018202">
        <property type="entry name" value="Ser_caboxypep_ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF350">
        <property type="entry name" value="CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00560">
        <property type="entry name" value="CARBOXYPEPT_SER_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00131">
        <property type="entry name" value="CARBOXYPEPT_SER_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 11" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7754511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="455" mass="51192" checksum="21F7474B462A2367" modified="2024-05-29" version="1">MTNELNLHPNLEVNILKSKTSSLEDELKVSESGFIEKRLSLSVLGDSGATVGDLAQHAGYFRLQHTIDARMFYFFFESRRAKADPVVIWLTGGPGCGSELALFYENGPFKLTNNLSLIWNDYGWDKVSNIIYVDQPTGTGFSYSSSYEDIRHDEDGVSNDLYSFLQAFFKAHPDYINNDFYITGESYAGHYIPAFAARVNRGNKNKEGIHINLKGFGIGNGLTHPGIQYQAYTDYALANNLISESDYNQINQRLPACERAINQCGATGSDMCQPAYSFCQQIFEDILSLTDNINYYDIRKKCKGSLCYDFSNLENFLSKSSVKAALGVPSYIDYVSCSDLVFRSMLNDWMRNLEVEIPALLEDKIQLLIYVGEYDLICNWLGNSRWVHAMSWSGQKDFMSASNVSFIVDGKEAGKIKNHGPLTFIKVHNAGHMVPMDQPRAALKMLELWTTGKLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-02-05" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GWH3</accession>
    <name>A0AAD5GWH3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PB1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_018919</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010002421">
        <property type="protein sequence ID" value="KAI7754826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05992">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045012">
        <property type="entry name" value="NLP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055081">
        <property type="entry name" value="NLP1-9_GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053793">
        <property type="entry name" value="PB1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000270">
        <property type="entry name" value="PB1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32002:SF35">
        <property type="entry name" value="PROTEIN NLP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32002">
        <property type="entry name" value="PROTEIN NLP8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22922">
        <property type="entry name" value="GAF_NLP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00564">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00666">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51745">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PB1" evidence="2">
        <location>
            <begin position="448"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="406"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="406"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7754826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="563" mass="62874" checksum="74F86B030DFEC086" modified="2024-05-29" version="1">MDLRAVSRREAICASQLTKPYSCDSKYLTSLLVFRETKPCLELGAANMDPIHQKIQDKIVAALKLLTFREQHVLVQFWSPHAVGKHQLLTTRNQPHGIGEIDVGLCLYRQDSESQSFVVDKDHEEEDRSPVARVFTRGLPEWTSDLESCTLKHFPQQESAICCNLHGYLALPVFDLITRLCVGVLELLTSSKYMSYAYEVQQVHTALKTENLISPQGFDFPALYVHNESRQNNLDKIYGILKKACEIHSLPLAQTXXXXXXHEKVIEKSCSSFDTRCVGKVCMSTTNLPFNVQDLSMWPFFKACRERHLDVSCGFVGKALLSRGSCFCGDVTKLSEEEYPLVHNARMNRGQVLNLVQTLKQNCAIGSGFELGDNSCIQVVRLPTDLSVNMEPDVIQISSDSVADNFTSSDAESSMPNPTNLSEEKTGVASTDAGEKSKKRKLESFPWMVTAEISFNDDMKVFQFPISSGLSYLKNEVSQSFKLKGKKLSLKYIDEDDDSILIACNEDLQSAMVATGSNNIITVTCLQSQQYQLNLCQINLCDTHCCLVDLKFGNSFVACGCIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GWN3</accession>
    <name>A0AAD5GWN3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Polygalacturonase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_010885</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 28 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010002115">
        <property type="protein sequence ID" value="KAI7755006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GWN3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004650">
        <property type="term" value="F:polygalacturonase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051801">
        <property type="entry name" value="GH28_Enzymes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000743">
        <property type="entry name" value="Glyco_hydro_28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31339:SF4">
        <property type="entry name" value="PECTIN LYASE-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31339">
        <property type="entry name" value="PECTIN LYASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00295">
        <property type="entry name" value="Glyco_hydro_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00710">
        <property type="entry name" value="PbH1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7755006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="204" mass="22734" checksum="5B1CF7C4FF544721" modified="2024-05-29" version="1">MWWDLWWNRTLKHTRGHLLEIMNSRNILISNLTFRNSPFWTIHPVYCSNVVIKDMTILAPLNAPNTDGIDPDSCTNVCIEDCYIESGDDLVAIKSGWDQYGTSMARPSSNIIIRRVSGTTPTCSGVGIGSEMSGGISNIRVENLHIRNSAAGIRIKTDIGRGGYIENITITNITMDKVKVPLRFSRGADDHPDNGWDPHALPRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GXE0</accession>
    <name>A0AAD5GXE0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">LIM zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_005609</name>
        <name evidence="6" type="ORF">M8C21_006492</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008277">
        <property type="protein sequence ID" value="KAI7741090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAMZMK010000356">
        <property type="protein sequence ID" value="KAI7756519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GXE0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051017">
        <property type="term" value="P:actin filament bundle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd09440">
        <property type="entry name" value="LIM1_SF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09441">
        <property type="entry name" value="LIM2_SF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000002">
        <property type="entry name" value="LIM domain and actin-binding 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24206">
        <property type="entry name" value="OS06G0237300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0440">LIM domain</keyword>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="5">
        <location>
            <begin position="8"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="5">
        <location>
            <begin position="106"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7741090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7756519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="200" mass="21609" checksum="AA2E10DA02919C91" modified="2024-05-29" version="1">MSFIGTQQKCKACGKTVYPVELLSADGIDYHKSCFKCSHCKGTLKLSNYSSMEGVLYCKPHFEQLFKESGNFNKNFTSPAKAADKLSPMLTKSPSKAAGMFSGTQEKCATCGKTAYPLEKVTVENQAYHKSCFKCSHGGCSLSPSNYAALEGILYCKHHFSQLFKEKGSYNHLIKSASIKRTAAAATAAAATPEAASIPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GXE3</accession>
    <name>A0AAD5GXE3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glycosyltransferase</fullName>
            <ecNumber evidence="6">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_003167</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">May glycosylate diterpenes or flavonols in leaves.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="6"/>
    <dbReference type="EMBL" id="JAMZMK010000861">
        <property type="protein sequence ID" value="KAI7755726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GXE3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080043">
        <property type="term" value="F:quercetin 3-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080044">
        <property type="term" value="F:quercetin 7-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000056">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000065">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926">
        <property type="entry name" value="GLUCOSYL/GLUCURONOSYL TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926:SF1417">
        <property type="entry name" value="UDP-GLUCURONOSYL_UDP-GLUCOSYLTRANSFERASE, UDP-GLYCOSYLTRANSFERASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00053747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7755726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="480" mass="54074" checksum="DD21303CE393D214" modified="2024-05-29" version="1">MDPMLTTEKKPHVVCIPLPSQSHVKAMLKLAKLLHHKGLQITFVNTEFVHQLLLESGGPHSLDGSPGFHFETIPDGVPRSSKDSITTTRLLLLQSVETNLLAPFIDLVTKLPNPPTCIISDGFMSAFTIDAAQNLGIPVMMYWTFAACAIMGFYQINSLIEKGLAPLKDESYLTNGYLDTVIEWVPGMEDICLKDFPMTWTTDANDKQLVFSKEAPQRSVKVPHHIFHTFDELETSTIKALSSIYSRVYTIGPLQLLLDQIPREKMQSESSSTHGYSLWKEEPECFQWLQSKEQNSVIYVNFGTTTEMSLNDVRELGWGLANSNHYFLWIIRSNLVEGESAVLPPEFEEHINKRGFIASWCSQEKVLNHPSVGGFLTHCGWGSTIESLSAGVPMICWPFAWDQKTNRRSICKDWGVGVEMGNEVKRDEVSRLVQELMGERGHIMRNKAKEWKEKARVATSPNGSSSLNVDNIVKEIMMHC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GYM5</accession>
    <name>A0AAD5GYM5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_008992</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000064">
        <property type="protein sequence ID" value="KAI7757764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GYM5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11660">
        <property type="entry name" value="SANT_TRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057625">
        <property type="entry name" value="TPR1-6-like_ubiquitin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031105">
        <property type="entry name" value="TRP_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21717:SF84">
        <property type="entry name" value="HOMEODOMAIN-LIKE, UBIQUITIN-RELATED DOMAIN PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21717">
        <property type="entry name" value="TELOMERIC REPEAT BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23603">
        <property type="entry name" value="Ubiquitin_TPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="2">
        <location>
            <begin position="345"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7757764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="598" mass="67046" checksum="C5FE735D889959AD" modified="2024-05-29" version="1">MVLKKRIYDGGFHGLLPPVIPKAPRSLRKRRLHNKLSEDGEISGFELLAAVADKLLQEQESESSTSSTGSKGKEQISILEEGVKHESLEVNVKAVMSDHHDPASGAESELVAHQTTNLDASLKESPNSENDSGSDHASIVTNSGFVKKVNPSLIMEAIEDKPGDISDANVNDNRDGIHHKAVEKQSGVKTYKDGLKPCVHTGVSNKSYTSADLPFYKDHVRSACFPRDRGNVNISIRDDDEKYFRYNNCNKNRLSVFGARSRAGYRRMRKMMPSRYWKVARKLHEYELQNGYSGGVKSFYHKRKKICMRERFQADAAIKRRKLFHNSSKKSYIKEASSESMSNLVKFSIKSFKVPELYVEVPETETVGSLKRSVMEAVKAILHGKLHVGVVVQGKKVRDDNRTLQQSGISQNCNLETVGFTLESSLPEVSPFLAREEAPSCNSQQWQSRPITDGCFSSSSLDPPLAISLDNHLEKNEESKSFADEVTEVTTQDVVEDSKALVVVPPLNTEVLSVVPLSETSAKRYEFSQRRTRRPFSVSEVEALVEAVETLGAGRWRDVKMRAFGEANHRTYVDLKVSRVGLQRFNFLFFCKQLACHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GZ64</accession>
    <name>A0AAD5GZ64_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">U-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_014341</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000125">
        <property type="protein sequence ID" value="KAI7757436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GZ64"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058678">
        <property type="entry name" value="ARM_PUB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23315:SF129">
        <property type="entry name" value="ARM REPEAT SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23315">
        <property type="entry name" value="U BOX DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25598">
        <property type="entry name" value="ARM_PUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="U-box" evidence="5">
        <location>
            <begin position="182"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="3">
        <location>
            <begin position="218"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="18"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="39"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="50"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF25598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7757436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="462" mass="49459" checksum="DEC0E5C3DBF7E7BA" modified="2024-05-29" version="1" fragment="single">SHTSNPGRFQTTRPYYTPPSSSSPSPSRLQKHLGRSMRTIRSTLNQSDYTNNNNTFPFLTNIQQPLSPPPSENFSDSVIDVHLQQLATNNKNNNNNNNNKLSKSSCSTTSAEEYLGISFSEFSGSDISGELQRLACLPEETSTDTNSNVIKSLEKPDGCDPMEIIENISSEDLQPTVKMCVESLSVSNSIVVKRSAAEKLRLLAKNREENRALIGESGAVPLLLPLLRCSDPWTQENAVTALLNLSLLDANKPLISDASTEVSKQNAACALLNLAMIDENKNSIGACGAIPPLVSLLINGSNRGKKDSLTTLYKLCSIKANKRRAVSAGVVKPLVELVVAAGEGGEEQGAVGMAEKALVVLCSVAAVEEGKNAIVEEGGIPALVEVIEDGVSVKGKEFSVVLCEGVNGARNRGLLVGEGVIPPLVALSQSGTAKAKHKLYAFLYIRTLNGKQKYVVNCEIHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2019-07-31" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A4P8PIN6</accession>
    <name>A0A4P8PIN6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">MADS-box transcription factor PISTILLATA-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">PI</name>
        <name evidence="9" type="ORF">M8C21_025404</name>
    </gene>
    <organism evidence="10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2018-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Virag E."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009686">
        <property type="protein sequence ID" value="KAI7734609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="MK098083">
        <property type="protein sequence ID" value="QCQ83104.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A4P8PIN6"/>
    <dbReference type="OrthoDB" id="1898716at2759"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00265">
        <property type="entry name" value="MADS_MEF2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1810.10">
        <property type="entry name" value="Transcription factor, MADS-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050142">
        <property type="entry name" value="MADS-box/MEF2_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033896">
        <property type="entry name" value="MEF2-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002487">
        <property type="entry name" value="TF_Kbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002100">
        <property type="entry name" value="TF_MADSbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036879">
        <property type="entry name" value="TF_MADSbox_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48019">
        <property type="entry name" value="SERUM RESPONSE FACTOR HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01486">
        <property type="entry name" value="K-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00319">
        <property type="entry name" value="SRF-TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00404">
        <property type="entry name" value="MADSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00432">
        <property type="entry name" value="MADS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55455">
        <property type="entry name" value="SRF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51297">
        <property type="entry name" value="K_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00350">
        <property type="entry name" value="MADS_BOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50066">
        <property type="entry name" value="MADS_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MADS-box" evidence="7">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="K-box" evidence="8">
        <location>
            <begin position="84"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="77"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7734609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="QCQ83104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="196" mass="22915" checksum="6AF659E2E7A5706E" modified="2019-07-31" version="1">MGRGKIEIKRIENTNNRQVTYSKRKNGIIKKAKEITVLCDANVSLVVSGSSGKMYEYCSPNTNLIDMLDRYQRLSGNKLWDAKHENLQNEIDRIKKENESMQIELRHLKGEDITSLTIEELIAFEDALENGLTNIREKKDEIPKIMRKHEQLLEEENKHLMYMVQQSEMAAMGDYQANEPFSFRVQPMQPNLHERM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BKZ3</accession>
    <name>A0AAD5BKZ3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Histone H3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">M8C21_005031</name>
        <name evidence="13" type="ORF">M8C21_026053</name>
        <name evidence="14" type="ORF">M8C21_026065</name>
        <name evidence="16" type="ORF">M8C21_030116</name>
    </gene>
    <organism evidence="14 17">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="10">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the histone H3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011990">
        <property type="protein sequence ID" value="KAI7725255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAMZMK010011990">
        <property type="protein sequence ID" value="KAI7725267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAMZMK010009947">
        <property type="protein sequence ID" value="KAI7733543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAMZMK010005701">
        <property type="protein sequence ID" value="KAI7752383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BKZ3"/>
    <dbReference type="SMR" id="A0AAD5BKZ3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22911">
        <property type="entry name" value="HFD_H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000078">
        <property type="entry name" value="Histone H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000044">
        <property type="entry name" value="Histone H3.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000164">
        <property type="entry name" value="Histone_H3/CENP-A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11426">
        <property type="entry name" value="HISTONE H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00622">
        <property type="entry name" value="HISTONEH3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00428">
        <property type="entry name" value="H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00322">
        <property type="entry name" value="HISTONE_H3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00959">
        <property type="entry name" value="HISTONE_H3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 10" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="12">
        <location>
            <begin position="44"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7725255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7725267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAI7733543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAI7752383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="136" mass="15302" checksum="772079AD5D777A78" modified="2024-05-29" version="1">MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRFRPGTVALREIRKYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVAALQEASEAYLVGLFEDTNLCAIHAKRVTIMPKDMQLARRIRGERA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BNI0</accession>
    <name>A0AAD5BNI0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">Glucose-methanol-choline oxidoreductase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_020448</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the GMC oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011621">
        <property type="protein sequence ID" value="KAI7726637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BNI0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016614">
        <property type="term" value="F:oxidoreductase activity, acting on CH-OH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.410.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051871">
        <property type="entry name" value="GMC_Oxidoreductase-Related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000172">
        <property type="entry name" value="GMC_OxRdtase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45968:SF36">
        <property type="entry name" value="(R)-MANDELONITRILE LYASE 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45968">
        <property type="entry name" value="OSJNBA0019K04.7 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00732">
        <property type="entry name" value="GMC_oxred_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00623">
        <property type="entry name" value="GMC_OXRED_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00624">
        <property type="entry name" value="GMC_OXRED_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0274">FAD</keyword>
    <keyword evidence="2 4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucose-methanol-choline oxidoreductase N-terminal" evidence="5">
        <location>
            <begin position="41"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="Glucose-methanol-choline oxidoreductase N-terminal" evidence="6">
        <location>
            <begin position="201"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS00623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7726637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="216" mass="23510" checksum="C993E5E99963651B" modified="2024-05-29" version="1" fragment="single">ERYLGFTYEATDFTPEKEYDYIIIGGGTAGCPLAATLSEKGRVLGGSSMINFGFYSRAEDFFYENSGIELDMSAVNRSYEWVENSIITRPARLRRFQTSMFNALLEAGVRPDNGFTVNHLQGTKVGGSIFDDSGRRHGAVELLSKGYPKNLKVVVHAIVDRIIFSKSNQSLAAVGVKYHDSKGTNHEVHVRTAGEVILSAGAIGSPQLLLLSGLGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BNI4</accession>
    <name>A0AAD5BNI4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_019922</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011914">
        <property type="protein sequence ID" value="KAI7725558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BNI4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000336">
        <property type="entry name" value="Cysteine-rich receptor-like protein kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052059">
        <property type="entry name" value="CR_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47973">
        <property type="entry name" value="CYSTEINE-RICH RECEPTOR-LIKE PROTEIN KINASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="33"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7725558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="331" mass="37288" checksum="0CDAF6447235BF3D" modified="2024-05-29" version="1">MLSVADKSTGSTELLQGPLKYSYGDLKVATDNFSDEHRLGGGVFGEVYKGTLKDGNIVAIKKTTMASISGKLEELKIICNVHHRHLIRLLGYCNKGPFLFLVHEYMDNGSLDQFLYGYKSLNLNWRQRFEIIYGTARGLAYLHEQYHVTIIHREIKTSNILLDNEYQPKIADFGMIRLLPEDKTHLSTKAAASMDSGYVAPEYAVHGQLSEKVDTYSFGIVVLEIISGKRCHYVEDDGSIGQNLLEYAWNLYDRGAYLNLVDERIDPTEYANEDAKKIIEIALMCTQSPVSARPAMSEVVTLLSDKSLDEIPHVRPTSHEDNINIKVDTFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BP43</accession>
    <name>A0AAD5BP43_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Chlorophyll a-b binding protein, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_003353</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">The light-harvesting complex (LHC) functions as a light receptor, it captures and delivers excitation energy to photosystems with which it is closely associated.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast thylakoid membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the light-harvesting chlorophyll a/b-binding (LHC) protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011497">
        <property type="protein sequence ID" value="KAI7726905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BP43"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009522">
        <property type="term" value="C:photosystem I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009523">
        <property type="term" value="C:photosystem II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016168">
        <property type="term" value="F:chlorophyll binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009765">
        <property type="term" value="P:photosynthesis, light harvesting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3460.10">
        <property type="entry name" value="Chlorophyll a/b binding protein domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001344">
        <property type="entry name" value="Chloro_AB-bd_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022796">
        <property type="entry name" value="Chloroa_b-bind"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21649">
        <property type="entry name" value="CHLOROPHYLL A/B BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00504">
        <property type="entry name" value="Chloroa_b-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103511">
        <property type="entry name" value="Chlorophyll a-b binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 12 13" id="KW-0148">Chlorophyll</keyword>
    <keyword evidence="3 13" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="8 13" id="KW-0157">Chromophore</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 13" id="KW-0602">Photosynthesis</keyword>
    <keyword evidence="13" id="KW-0603">Photosystem I</keyword>
    <keyword evidence="11 13" id="KW-0604">Photosystem II</keyword>
    <keyword evidence="5 13" id="KW-0934">Plastid</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 13" id="KW-0793">Thylakoid</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="binding site" evidence="12">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>chlorophyll a</name>
            <dbReference type="ChEBI" id="CHEBI:58416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>chlorophyll a</name>
            <dbReference type="ChEBI" id="CHEBI:58416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="12">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>chlorophyll b</name>
            <dbReference type="ChEBI" id="CHEBI:61721"/>
            <label>1</label>
        </ligand>
        <ligandPart>
            <name>Mg</name>
            <dbReference type="ChEBI" id="CHEBI:25107"/>
        </ligandPart>
    </feature>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR601344-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7726905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="156" mass="16751" checksum="C06C7CAAD730E2AE" modified="2024-05-29" version="1" fragment="single">MASLAAASLGVSEMRGNPLNFSGASRAAPSPSTPATCKTVALFQKKKPAPPPKSKPAAVTPATDELAKWYGPERRIFLPEGLLDRSEIPEYLNGEVAGDYGYDPFGLGKKPEDFAKYQAYELIHARWAMLGAAGCIIPEAFNKYGANCGPEAVWFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BPE8</accession>
    <name>A0AAD5BPE8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">FACT complex subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">M8C21_008079</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">AA19_3_7</strain>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the FACT complex, a stable heterodimer of SPT16 and SSRP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the peptidase M24 family. SPT16 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011435">
        <property type="protein sequence ID" value="KAI7727127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BPE8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035101">
        <property type="term" value="C:FACT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01091">
        <property type="entry name" value="CDC68-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.150:FF:000004">
        <property type="entry name" value="FACT complex subunit SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.210:FF:000002">
        <property type="entry name" value="FACT complex subunit SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000017">
        <property type="entry name" value="FACT complex subunit SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.350.10:FF:000006">
        <property type="entry name" value="FACT complex subunit SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.230.10:FF:000005">
        <property type="entry name" value="FACT complex subunit spt16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.350.10">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.210">
        <property type="entry name" value="FACT complex subunit Spt16p/Cdc68p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029149">
        <property type="entry name" value="Creatin/AminoP/Spt16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029148">
        <property type="entry name" value="FACT-SPT16_Nlobe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056595">
        <property type="entry name" value="Fact-SPT16_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048969">
        <property type="entry name" value="FACT_SPT16_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013953">
        <property type="entry name" value="FACT_SPT16_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013719">
        <property type="entry name" value="RTT106/SPT16-like_middle_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040258">
        <property type="entry name" value="Spt16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033825">
        <property type="entry name" value="Spt16_M24"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13980">
        <property type="entry name" value="CDC68 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13980:SF15">
        <property type="entry name" value="FACT COMPLEX SUBUNIT SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14826">
        <property type="entry name" value="FACT-Spt16_Nlob"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24824">
        <property type="entry name" value="PH_SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08512">
        <property type="entry name" value="Rttp106-like_middle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08644">
        <property type="entry name" value="SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21091">
        <property type="entry name" value="SPT16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01285">
        <property type="entry name" value="FACT-Spt16_Nlob"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01287">
        <property type="entry name" value="Rtt106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01286">
        <property type="entry name" value="SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 11" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 11" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8 11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3 11" id="KW-0235">DNA replication</keyword>
    <keyword evidence="9 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 11" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="FACT complex subunit SPT16 N-terminal lobe" evidence="13">
        <location>
            <begin position="26"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="FACT complex subunit SPT16 middle" evidence="14">
        <location>
            <begin position="552"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="domain" description="Histone chaperone RTT106/FACT complex subunit SPT16-like middle" evidence="15">
        <location>
            <begin position="831"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="484"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="955"/>
            <end position="1065"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="484"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="972"/>
            <end position="985"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="992"/>
            <end position="1010"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1011"/>
            <end position="1031"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00062995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM01287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAI7727127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="1065" mass="119228" checksum="367391A776A68CFC" modified="2024-05-29" version="1">MADHRNGGNHPAASKGAGGASGGYTIDANTLTRRLQSLYSHWREHRDELWGSCNAFAVATPPPSEDLRYLKSSALNIWLLGYEFPETIMVFSEKQIHFLCSQKKASLLDVVKKSAKEAVNVDVVMHVKAKNDDGVTQMDAILRSIESVDSPVLGYITREAPEGKLLETWTEKIKSSGLQLSDVTNGLGDLFAFKDSGELTNVKKAAYLTASAMKQFVVPKLEKVIDEEKKVTHSSLMDDTEKAIMEPARIKVKLKADNVDICYPPIFQSGGNFDLRPSASSNDDHLYYDSASVIICALGSRYNSYCANVARTFLIDSNATQSNAYQVLLRAHEAAVGALYPGTKACDVYKAALAVVEQEAPELTSNLTKSAGTGIGLEFRESGLNLNEKNERILKPGMVLNVSLGFQNMQTKSSKAKSQNYALLIADTVIVTATGHEVVTSLSSKAFKDIAYSFNDGEEEEYERPQVKVDSKVNEALYSKATLRSDNHEMSKEELRRQHQAELARQKNEETAQRLAGGKSATGEGRSSSRASNDLIAYKNVNDVPPPRDLMIQVDQRNEAILIPLYGSMVPFHVATVKTVSSQADTSRNCYIRIIFNVPGTPFSSQDPNSLKNQTDIFLKEVSFRSKDPRHISEVVQQIKTLRRNVVSRESERAERASLVTQEKLVLAGNKFKPIRLTDLWIRPTFPGRGRKLPGTLEAHDNGFRYSTSRADERVDILFGNIKHAFFQAAEKEMITLLHFHLHNHIMVGNKKTKDVQFYVEVMDVVQTLGGGKRSAYDPDEIEEEQRERDRKNKINMNFQNFVNRVNDLWGQPKFKSFDLEFDQPLRELGFHGVPHKASAFIVPTSSCLVELIENPFLVVTLSEIEIVNLERVGLGQKNFDMAIVFKDFKRDVLRIDSIPSTSLDGIKEWLDTTDIKYYESRLNMNWRAILKTITDDPQNFIDEGGWEFLNLEASDSDSDASQESDQGYEPSDVEPESESEDEGSDSASLVESDDDVEEGSEEEGSEEEEGKTWEELEREASNADREKGDESDSEEERKRRKMKAFGKSRAGPSSSAPKRPKFRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BQ56</accession>
    <name>A0AAD5BQ56_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_019325</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011345">
        <property type="protein sequence ID" value="KAI7727576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BQ56"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1050.10">
        <property type="entry name" value="At2g23090-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045230">
        <property type="entry name" value="MBS1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026939">
        <property type="entry name" value="ZNF706/At2g23090_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21213">
        <property type="entry name" value="GEO09665P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21213:SF0">
        <property type="entry name" value="ZINC FINGER PROTEIN 706"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118359">
        <property type="entry name" value="Expressed protein At2g23090/F21P24.15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="35"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7727576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="68" mass="7582" checksum="9E132697938EB88A" modified="2024-05-29" version="1">MTRGKQKIEAQRKNAEKNSKSKGSQLEHRAVALKVTCPICKVQLANQNQLGDHYGSKHPKEKPPSNSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BQ86</accession>
    <name>A0AAD5BQ86_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PPM-type phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_003414</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011339">
        <property type="protein sequence ID" value="KAI7727623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BQ86"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="1">
        <location>
            <begin position="29"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7727623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="172" mass="18953" checksum="11738BCD66264880" modified="2024-05-29" version="1" fragment="single">MDSLCCLQLRGGPSSYSSGKGKNHEGDIKYGFELVKGKANHPMEDYHVARFVEIDDHELGLFAIYDGHSGDLVPSYLQKHLFDNILNESEFWVDPKRSISKAYEKTDAAILTDDSDLGRGGSTSVTAILMNGQRLWVANLGDSRAVISKGGEAIQLSTDHEPSTERVSIEHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BQC0</accession>
    <name>A0AAD5BQC0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_011922</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011354">
        <property type="protein sequence ID" value="KAI7727565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BQC0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7727565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="20" mass="2375" checksum="46DC7246F57EBEE2" modified="2024-05-29" version="1">MASSFEFSWMVRSSLFSVYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BQD3</accession>
    <name>A0AAD5BQD3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_003413</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011339">
        <property type="protein sequence ID" value="KAI7727622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BQD3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7727622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="58" mass="6429" checksum="A615487981A04847" modified="2024-05-29" version="1" fragment="single">MAKNLLKQEHDLATDLLEAIYHIVLELLDILKKMYDKGGKGGKSGMKSPDGFAKLKGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BSG4</accession>
    <name>A0AAD5BSG4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_012991</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011341">
        <property type="protein sequence ID" value="KAI7727611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BSG4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000047">
        <property type="entry name" value="Myb transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000394">
        <property type="entry name" value="MYB transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051953">
        <property type="entry name" value="Plant_SW-associated_TFs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47997">
        <property type="entry name" value="MYB DOMAIN PROTEIN 55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47997:SF34">
        <property type="entry name" value="TRANSCRIPTION FACTOR MYB86-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="8">
        <location>
            <begin position="9"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="7">
        <location>
            <begin position="9"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="7">
        <location>
            <begin position="62"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="8">
        <location>
            <begin position="66"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7727611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="326" mass="37061" checksum="6D9259F616D67061" modified="2024-05-29" version="1">MGRHSCCVKQKLRKGLWSPEEDEKLLKYISRFGVGCWSSVPKHAGLQRCGKSCRLRWINYLRPDLKRGTFSQQEEDRILNLHQVLGNRWAQIAAQLPGRTDNEIKNYWNSSLKKKLVKQGIDPNTHKPFTDKDYAKDKKIEFYASSSSSIPMAAELEHALHFNNGGIMLSSSYMDTGLIRDTLLSKSACDPLFLVEFQSGIDPTNYNSNLLAQFHQTNYETSLPNLASFDHHDVKMTTNSFMINEVRESSSTTNSLSAGFQPITYEGEMFQFNGANVKSEQGCVGQWEHNMHEVQVNNASDISVSTQQGHYLGNTLMSTIRYDWPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BSN2</accession>
    <name>A0AAD5BSN2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Anaphase-promoting complex subunit 4 WD40 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_005992</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011329">
        <property type="protein sequence ID" value="KAI7727733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BSN2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016593">
        <property type="term" value="C:Cdc73/Paf1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051510">
        <property type="entry name" value="SKI8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44090:SF1">
        <property type="entry name" value="SUPERKILLER COMPLEX PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44090">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="10"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="56"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="161"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="196"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="239"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7727733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="320" mass="34303" checksum="3C80EBDA8FDB2FC1" modified="2024-05-29" version="1">MKLAWLRSLESAHQDCVWACTWVPATDRSGGLLLTGSLDETVKLWNPEDLSVVRTNTGHCLGVVSVAAHPSGRIGASASMDSFIRVFDVESNNTIATLEAPPSEVWQMQFDPRGTTLAVAGGSSASVKLWDTAEWKLVSTLSIPRPEGAKPSDKGSSKKFVLSVAWSPSGRQLACGSMDGSISVFDVQRAKFLHHLEGHTMPVRSLAYSPLDPRVLVSGSDDECIHMYDAEGKTLFSSMSGHSSWVLSVDVSPDGAAIASGSSDRTVRLWDPKMRAAVQTMTNHTDQVWGVKFEAVGGGARGSLLASVSDDKSISLYNYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BTD1</accession>
    <name>A0AAD5BTD1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glutathione S-transferase</fullName>
            <ecNumber evidence="5">2.5.1.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_007133</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Is involved in the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
            <location evidence="5">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the GST superfamily. Tau family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="5"/>
    <dbReference type="EMBL" id="JAMZMK010011267">
        <property type="protein sequence ID" value="KAI7728138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BTD1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009407">
        <property type="term" value="P:toxin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03185">
        <property type="entry name" value="GST_C_Tau"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03058">
        <property type="entry name" value="GST_N_Tau"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000044">
        <property type="entry name" value="Glutathione S-transferase GSTU6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000016">
        <property type="entry name" value="Glutathione S-transferase U9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045074">
        <property type="entry name" value="GST_C_Tau"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045073">
        <property type="entry name" value="Omega/Tau-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11260:SF710">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11260">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE, GST, SUPERFAMILY, GST DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14497">
        <property type="entry name" value="GST_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13417">
        <property type="entry name" value="GST_N_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01152">
        <property type="entry name" value="Main.3:_Omega-_and_Tau-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1" id="KW-0216">Detoxification</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="6">
        <location>
            <begin position="5"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="7">
        <location>
            <begin position="91"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU369102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7728138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="230" mass="26486" checksum="026E44CE75B49D53" modified="2024-05-29" version="1">MGNESEIKLLGTPSSPFVNRVQFALNLKSIEYEYIEQNLAPKSEFLLKSNPVHKKVPVLLHADKPPICESQIIIEYLDEIRPDLFQLLPSVSLDRANCRFWATYIDTKFFPLYEELRKTTKKVGQEEIKEQMIEATQLLEEAFIKSSNGKAYFGGDQVGYLDVVLGCFIRWTKHVDELNDFNTFDEVRTPKLAKWAKTIASHEALKSVTPGKETLENFFAMLNVYKPPYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BUJ5</accession>
    <name>A0AAD5BUJ5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein ENHANCED DISEASE RESISTANCE 2 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_022002</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010969">
        <property type="protein sequence ID" value="KAI7729584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BUJ5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045096">
        <property type="entry name" value="EDR2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009769">
        <property type="entry name" value="EDR2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12136">
        <property type="entry name" value="ENHANCED DISEASE RESISTANCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12136:SF107">
        <property type="entry name" value="PLECKSTRIN DOMAIN, START DOMAIN, PROTEIN ENHANCED DISEASE RESISTANCE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07059">
        <property type="entry name" value="EDR2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein ENHANCED DISEASE RESISTANCE 2 C-terminal" evidence="2">
        <location>
            <begin position="111"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7729584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="326" mass="36228" checksum="FB709C1A2EE1A6E3" modified="2024-05-29" version="1" fragment="single">LKAYTNAKSTVLDKEVSLSVNDFDEEDDDDDDDDEVADQFYDAISAYSFSSSSEDDDYDDTDYDDTQHVSKDQSMDWTVSSLDKKSNPDPGSSHLCGSMCEAKSENDTNCWMSPSGKGFMIRGKTYLKDNTKVSGGDPLLKLIAVDWFKINNPISKVALHPKCLLQSEAGKKLPFVLVMNLQVPAKPNYSLVLYFCSDKPVVEDSLLGRFIDETDTFRNSRFKLIPSIVEGYWMVKRAVGTKACLLGKAVSCNYIRQDNFLEIDVDIGSSSVARNVIGIVLGYVTSIVVDLAILIEGKEEDELPEYILGTVRLNRVRPESAVPLES</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BVZ4</accession>
    <name>A0AAD5BVZ4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_007776</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011013">
        <property type="protein sequence ID" value="KAI7729364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BVZ4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7729364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="73" mass="8684" checksum="14699E0908DBE331" modified="2024-05-29" version="1">MLDVWLNHNLLQNCTDHIDMKNRRSGSTFISGAIQLQLQPFGKLYTTKRVENQKSYQQQQHGHFDLLTYEKYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-06-18" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BWQ8</accession>
    <name>A0AAD5BWQ8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">M8C21_019930</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">AA19_3_7</strain>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010010565">
        <property type="protein sequence ID" value="KAI7731041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000363">
        <property type="entry name" value="Leucine-rich repeat family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000328">
        <property type="entry name" value="Leucine-rich repeat protein kinase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000542">
        <property type="entry name" value="Leucine-rich repeat protein kinase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000453">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase HSL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45974:SF287">
        <property type="entry name" value="CONCANAVALIN A-LIKE LECTIN_GLUCANASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45974">
        <property type="entry name" value="RECEPTOR-LIKE PROTEIN 55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="14 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="477"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="549"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="577"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="20">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="20">
        <location>
            <position position="869"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAI7731041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="869" mass="95685" checksum="EB3808B56D9B784D" modified="2024-05-29" version="1" fragment="single">TISNAVRRRNLWLTWMKNGSDGDGVTVVALQALKDAWQGTPPSWKDGSDPCGDDWDGITCTNSRVTWITLSNMGLSGTLPGDIGQFTELLVLDLSYNKGLKGSLTSAIGNLAKLTNLILVGCDFNGPIPNSIGNLERLRYLSLNSNGFTGSIPASIGQLKNLYWLDLSDNQLTGTLPVSDGITPGLDMLTATKHFHLGDNQLSGEIPLGLFNSNMTLYHLRLDRNLLSGDIPSNINNLTNVTEMYLSNNRLTGLVPNLTGMNRLNYLNNRINGSLDISDNPSRQLELVDLRLNVIYDFRQRKQYTIGLILVDNPICMESGIDFAHFQQKLLPRTRPQGTTAHTLHVVLTLLQVLTVNAHIHSPAXXXXLINATIYGSLHDLLMSFFKNASLPVDSLSLSNPSRNLEDYLVIRLEVFPSGQPTFNRTGIIGISFALSNQTFKPSKTDFNTYTFVAENYKFLSESEIGVAKSKTSNTGIIIGSAVGGCVLVVLLVLAGMYAIRQKGRAEKAVQHNQPFAQWDSTGASGGVPQLKGARSFTFEELKKYTNNFSEDSLLGAGGYGNVYKGSLPNGQLIAIKRARQGSTQGGLEFKAELELLSRVHHKNVVGLIGFCFDQGEQMLVYEYIVNGTLKDSLSGRSGIRLDWMRRLKIALGAARGLQYLHDLADPPIIHRDIKTNNILLDQRLVAKVADFGLSKPLSDANRTHITTQVKGTMGYMDPEYYMTQQLTEKSDVYSFGVVLLELITARNPIEKGRYIVREVKQAMDKNKELYNLHEVLDPTIGLSNQLKGLEMFVDLSLRCVEEKGTQRPGMSEIVKELESIMTFIGLNPGSEPSTSANYEGNGKDFSHPYSNDSLFAYSGGFLPPKLVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BWX8</accession>
    <name>A0AAD5BWX8_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_009732</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010489">
        <property type="protein sequence ID" value="KAI7731253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BWX8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7731253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="257" mass="29638" checksum="6360B224192E3A20" modified="2024-05-29" version="1">MKRLYRRSWVNQARHQRSAYLRLKALGYSNGEVASTHDPCTQYHSPPLKLLRLINQLVSVSTNHYEVDVFFTPRELLRYKSQEGATVLLLVWDDKTPQSTQLRDGFLAFCNLLIERFETQLSTPAPTQSTKLIPLGLFNGHQVEYKWFFYDQTTWMTYRGCKRNDLSANTFGRVSAYHEFVKEDEESKMLCVQDMSQIAQTSLNIGGQLVASRAAQTRIADHQTIYHVLNQEDQTFCRCRSNGLLVLYAFYDQGAGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BXM1</accession>
    <name>A0AAD5BXM1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_019904</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010806">
        <property type="protein sequence ID" value="KAI7730311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BXM1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46151">
        <property type="entry name" value="NEP1-INTERACTING PROTEIN-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46151:SF28">
        <property type="entry name" value="ZINC FINGER, RING_FYVE_PHD-TYPE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="39"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="8">
        <location>
            <begin position="170"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7730311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="215" mass="23944" checksum="3FFCFC73DA9F57F3" modified="2024-05-29" version="1">MNNCLHSIKLNMISSSLEHDGHHYGDKLKELFTSILSHVFFKLVLLIFIFHFGVVGGTIGAVGGALIGSKNKMNFLHDVITGAVSGIVLSHKIIMVTFDHFMHLDHDDEHSCFFHLIDIVACDLESKLLRDQDHIFLITKVSSCEKLVKLAKIEISNHDVVDSSGNATCCSICLQDFELGNSAGIFPKCEHKFHPECISQWLLTHNSCPVCRTTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-06-18" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BZP0</accession>
    <name>A0AAD5BZP0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_028918</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the BBP/SF1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010296">
        <property type="protein sequence ID" value="KAI7732164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048024">
        <property type="term" value="P:regulation of mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02395">
        <property type="entry name" value="KH-I_BBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.60.10:FF:000011">
        <property type="entry name" value="splicing factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000047">
        <property type="entry name" value="splicing factor-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045071">
        <property type="entry name" value="BBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055256">
        <property type="entry name" value="KH_1_KHDC4/BBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032570">
        <property type="entry name" value="SF1-HH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047086">
        <property type="entry name" value="SF1-HH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11208">
        <property type="entry name" value="RNA-BINDING PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11208:SF151">
        <property type="entry name" value="ZINC FINGER, CCHC-TYPE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22675">
        <property type="entry name" value="KH-I_KHDC4-BBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16275">
        <property type="entry name" value="SF1-HH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RRM" evidence="12">
        <location>
            <begin position="452"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="129"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="380"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="529"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="598"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="729"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="17"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="27"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="399"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="531"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="627"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="653"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="731"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="750"/>
            <end position="766"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7732164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="781" mass="83995" checksum="B57A46165F4A87C2" modified="2024-05-29" version="1">MDSLPSQTLTPSSPSSTPTPPPPPSDPHLTQPDPTSHNNLTTTTTNNLPLDDDTTTRRRRRSRWDPPPTDTEPTDTKKRKSRWADDDPKPVFQLPDFMKDFTGGMDLDPEIQPLNARLMEISRILQSGQLLDDRPDGARSPSPDPVYDNIGTRINTREYRARERLNKERQDIISQIIKRNPAFKPPADYRPPKLQKKLYIPMKEYPGYNFIGLIIGPRGNTQKRMEKETGAKIVIRGKGSVKEGRFGQKRDMKYDPSENEDLHVLVEAETQEGLDAAAGMVEKLLHPVDEGLNEHKRQQLRELAALNGTIRDDEYCRLCGEAGHRQFACPSRLSTFKSDVLCKICGDGGHPTIDCPVKGTGGKKMDDEYQNFLAELGGTLPEGVTKQSNPLPIMGSGSGSSSSGSNPPWAGGNSSNGSSAGLGVSAHPGLGSTAAKSGTGFGLGFGKEIDDTNLYIGYLPPTFEDDALIRLFSPFGEIVMAKVIRDKVTGLSKGYGFVKYSDVSQANQAIAGMNGYRLDGRVIAVRVAGKPPQPTVPPGPPAPATPAAPVPAYXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAGGYPSQQMASGGPVGSAGPPGSYVTAPVPWGQPPPPPPPPYASYAQPPPPPPPGSNVYSSFTGQHMPSYVQYPPPNSSSTETKLSYLGGLHSQNSVPAQSPASNVYGNSLAGTAPNAQSAYPSASMSYASYYSVPPPPPPPATEQPSFARAPWSSNPPVSSAEQSQQTAYGAESEYEKFMAEMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C152</accession>
    <name>A0AAD5C152_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_006171</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the major facilitator superfamily. Phosphate:H(+) symporter (TC 2.A.1.9) family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010065">
        <property type="protein sequence ID" value="KAI7733207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C152"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051119">
        <property type="term" value="F:sugar transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17358">
        <property type="entry name" value="MFS_GLUT6_8_Class3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044775">
        <property type="entry name" value="MFS_ERD6/Tret1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050549">
        <property type="entry name" value="MFS_Trehalose_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48021">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48021:SF23">
        <property type="entry name" value="SUGAR TRANSPORTER ERD6-LIKE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="118"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="144"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="213"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="252"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="280"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="314"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="354"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="386"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="1"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7733207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="420" mass="45370" checksum="4D32B40C64C3085C" modified="2024-05-29" version="1">MSFRDDNEDGRSDRDLRKPFLHTGSWYRMGSRQSSMMTSSQMIRDRSVSVLACVLIVALGPIQFGFTGGYSSPTQAAITRDLNLTVSEYSLFGSLSNVGAMVGAIASGQIAEYIGRKGSLMIAAIPNIIGWLCISFATDTSFLYMGRLLEGFGVGIISYTAKMGMTDDFEASLQVLRGFDTDITVEVNEIKRSVASSNRRTAIRFSDLKQRRYWFPLMIGIGLLVLQQSSGINGVLFYSSNIFQTAGISSSDAATCGLGAIQVIATAVATWLVDKTGRRILLTVSSCGMTFSLVVVAASFFVKSFVEESSSLYAAMGILSLVGVVGMVIAFSLGMGPIPWVIMSEILPVNIKGLAGSIATLCNWFIAWLITMTAPLLLDWSGGGTFTLYMLMCAATVVFTTILVPETKGKTLEEIQFSLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C165</accession>
    <name>A0AAD5C165_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_005244</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the plant CAR protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010288">
        <property type="protein sequence ID" value="KAI7732199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C165"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009738">
        <property type="term" value="P:abscisic acid-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04038">
        <property type="entry name" value="C2_ArfGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044562">
        <property type="entry name" value="CAR1-11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45933:SF28">
        <property type="entry name" value="C2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45933">
        <property type="entry name" value="PROTEIN C2-DOMAIN ABA-RELATED 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0938">Abscisic acid signaling pathway</keyword>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="8" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="12">
        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7732199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="166" mass="19190" checksum="2849B809C5AB0D2B" modified="2024-05-29" version="1">MSMSQLLGLLRVHIHRGVNLAIRDLRSSDPYVIIRMGKQKLKTRVVKKNLNPVWDEDLTLSIVEPLPVKLEVYDWDILSADDKMGDAEFDFTPFWEAVKMNFEDLPNDTIITTVKPTRTNCLAEESYITWTNGRVVQNMVLRLRNVECGEIELKLSWINVPGCRNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C196</accession>
    <name>A0AAD5C196_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Pectinesterase inhibitor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_005036</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>[(1-&gt;4)-alpha-D-galacturonosyl methyl ester](n) + n H2O = [(1-&gt;4)-alpha-D-galacturonosyl](n) + n methanol + n H(+)</text>
            <dbReference type="Rhea" id="RHEA:22380"/>
            <dbReference type="Rhea" id="RHEA-COMP:14570"/>
            <dbReference type="Rhea" id="RHEA-COMP:14573"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17790"/>
            <dbReference type="ChEBI" id="CHEBI:140522"/>
            <dbReference type="ChEBI" id="CHEBI:140523"/>
            <dbReference type="EC" id="3.1.1.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the pectinesterase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the PMEI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009947">
        <property type="protein sequence ID" value="KAI7733548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C196"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004857">
        <property type="term" value="F:enzyme inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030599">
        <property type="term" value="F:pectinesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042545">
        <property type="term" value="P:cell wall modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15798">
        <property type="entry name" value="PMEI-like_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.40">
        <property type="entry name" value="Invertase/pectin methylesterase inhibitor family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035513">
        <property type="entry name" value="Invertase/methylesterase_inhib"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000070">
        <property type="entry name" value="Pectinesterase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006501">
        <property type="entry name" value="Pectinesterase_inhib_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01614">
        <property type="entry name" value="PME_inhib"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31707">
        <property type="entry name" value="PECTINESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01095">
        <property type="entry name" value="Pectinesterase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04043">
        <property type="entry name" value="PMEI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00856">
        <property type="entry name" value="PMEI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101148">
        <property type="entry name" value="Plant invertase/pectin methylesterase inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0063">Aspartyl esterase</keyword>
    <keyword evidence="6" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042076731" description="Pectinesterase inhibitor domain-containing protein" evidence="8">
        <location>
            <begin position="27"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="Pectinesterase inhibitor" evidence="9">
        <location>
            <begin position="30"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7733548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="414" mass="46036" checksum="774FEAB03C4EC00E" modified="2024-05-29" version="1">MASFNLFLSILLSVIMLLSLSTLSMQLNEETDGDINRWCQTTPHPNQCNYFLSRGPHRAPRHRSDFRLMALQAAIDNANEAQGCTKKLGKMCRTSATKAVWNDCYRLLNNTVFQLNQTVTGLKNNQSTNFDTQTWLSAALTNLQTCITGCNEMNLTDFAKPIATSNLTEMISNSLAINHQFLKQKPASNQTNDDFPTWFSRHDRRLLATVSLQSLANVTVSQVSGNRFMTIQSALDYAGSIDRQNKRFIIYIKAGVYKENIDIVNGLNNIMFVGDGIRYTIITGNRSAAGGSTTYGSATVVPAPDLIPVLGSVQTYLGRPWQQYSRTVFMKTYLDGFINPKGWLPWGNTDFAFTTLYYGEYGCFGNGSSVKNRVNWTGYHGDMSLDDAQQFTVSGLIAGRMWLPATGVPFISGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C1M7</accession>
    <name>A0AAD5C1M7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Dehydrogenase E1 component domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_022575</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010030">
        <property type="protein sequence ID" value="KAI7733320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C1M7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016624">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009083">
        <property type="term" value="P:branched-chain amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050771">
        <property type="entry name" value="Alpha-ketoacid_DH_E1_comp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001017">
        <property type="entry name" value="DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43380:SF11">
        <property type="entry name" value="2-OXOISOVALERATE DEHYDROGENASE SUBUNIT ALPHA 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43380">
        <property type="entry name" value="2-OXOISOVALERATE DEHYDROGENASE SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00676">
        <property type="entry name" value="E1_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dehydrogenase E1 component" evidence="3">
        <location>
            <begin position="30"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7733320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="99" mass="11489" checksum="A7DF0363019CB575" modified="2024-05-29" version="1" fragment="single">DGIVVRRKAYGIPSIQVDGNDTLAICSAALTYRAGHHSTSDDSTMYRTVEEIEWWRHDQNPVKIFKKWFGNKGWWSNEAESQHQNIIRKQPKQFKLQSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C3P3</accession>
    <name>A0AAD5C3P3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_014505</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009983">
        <property type="protein sequence ID" value="KAI7733446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C3P3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012862">
        <property type="entry name" value="DUF1635"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33431">
        <property type="entry name" value="ENABLED-LIKE PROTEIN (DUF1635)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33431:SF12">
        <property type="entry name" value="HIGH MOBILITY GROUP BOX PROTEIN, PUTATIVE (DUF1635)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07795">
        <property type="entry name" value="DUF1635"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="46"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7733446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="325" mass="35717" checksum="3CEF163F71D4E3E1" modified="2024-05-29" version="1">MEEFASLWSYQENIDVLKEKLLYTTIELEAVKAETNEEMRRNTESMKQLLHMLKIACQERDEAKDQLQKLLNKFMSSNDQQMFNVTNASVPNCFDQVQQPHQGALMMPAKAHSSITESNSLSEAYNQSSPPVDSLFDPISSPEFSNINVETTFVQDYNQNVGFSNGLNGSENVQKVDQATLVMENMIKGKTLPQKGSFLQAVVEAGPLLQNLLLAGPLPRWRNPPPLKSFHIPPMMAAVDQTTTVQKQDVTHPNKLLTSPTTLLKQSQPYAEMACGSSSQMMAGGCIGGSGGVLSFGDVNFQGRMMAACPGGGTLGSIAKRQRLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C547</accession>
    <name>A0AAD5C547_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_032203</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009785">
        <property type="protein sequence ID" value="KAI7734196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C547"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7734196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="90" mass="10192" checksum="AF2F37347EB3834F" modified="2024-05-29" version="1">MATIAGARDRGATEESEDLIEKLNFRRGRGMGSLYSLYSVSRQNHPLMDAVVRCLHIISLYHPDLWLSPAKRIRPPIVDAARTMKLCQLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C5H5</accession>
    <name>A0AAD5C5H5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RBR-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="5">2.3.2.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">M8C21_017830</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">AA19_3_7</strain>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Might act as an E3 ubiquitin-protein ligase, or as part of E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.31"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the RBR family. Ariadne subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.31" evidence="5"/>
    <dbReference type="EMBL" id="JAMZMK010009476">
        <property type="protein sequence ID" value="KAI7735472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C5H5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22584">
        <property type="entry name" value="Rcat_RBR_unk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031127">
        <property type="entry name" value="E3_UB_ligase_RBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002867">
        <property type="entry name" value="IBR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11685">
        <property type="entry name" value="RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01485">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00647">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="29"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="15">
        <location>
            <begin position="123"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="14">
        <location>
            <begin position="127"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAI7735472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="325" mass="36908" checksum="CD713545958F27D3" modified="2024-05-29" version="1">MFTGVINFTFFTLPKPSSSLLLLLSTEDICYTVVVAAAPLALLIIASLLYSKNKKKKKEEIIATDIDDEEIIWAPASDVFFEEDEDARMLEADAKYAEELQLQEVLLASQNSTHETSLSSLLTQVKCQICLEKQESWQMFMNSSCSHSFCYDCTAKHATTQIQARHKTVACPGINCNSTLDGNTLRLIIPRETLIKWDEFLCESMIPESHKLYCPFTDCSVLLINDGTNNSVTKIKCPVCRQSFCKACRVPWHSKLSCKKFGKLKVKDDEMAVALAKKKNWKRCPNCSFFVEKTEGCVHITCRCKYEFCYTCGAKWSHGHYECKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C717</accession>
    <name>A0AAD5C717_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_022732</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009328">
        <property type="protein sequence ID" value="KAI7736282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C717"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11393">
        <property type="entry name" value="bHLH_AtbHLH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000032">
        <property type="entry name" value="Transcription factor bHLH123 family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045239">
        <property type="entry name" value="bHLH95_bHLH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045843">
        <property type="entry name" value="IND-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16223:SF238">
        <property type="entry name" value="TRANSCRIPTION FACTOR BHLH114"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16223">
        <property type="entry name" value="TRANSCRIPTION FACTOR BHLH83-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="8">
        <location>
            <begin position="250"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="232"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7736282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="371" mass="40391" checksum="09D63EDB0A2D7F97" modified="2024-05-29" version="1">MAEEFQAEVCGENWWSSPITTFNANANSSFMGSYGGGCCQNNFMNMKTRSTDESSGCCGTAILPDSASQLMGSSPSTTTNNIWYQALLVNGRTGESFSQTLPEILNGLPNSGQNSSNFGMDQDQASNFITNSSDCTGDFGSNSYGYPSSLLQTLFDGASSSPPPPPPPDVANYIQVNSLNNFDQVPSMAFLCNPSALDVNQAGFLSSKQAYEEKPKCPNIKSQDDEIQDNMGSSVKKGSMDSTTFKRPRLETPSPLPTFKVRKEKLGDRVTALQQLVSPFGKTDTASVLHEAIEYIKLLHDQVNLLSSPYMKNGANMQVQQRSDKVEDNNEGNRLQDLRSRGLCLVPVSSTFPVTTDTTPDYWTSGFRSTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C7S3</accession>
    <name>A0AAD5C7S3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">mannan endo-1,4-beta-mannosidase</fullName>
            <ecNumber evidence="4">3.2.1.78</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_025727</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random hydrolysis of (1-&gt;4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.</text>
            <dbReference type="EC" id="3.2.1.78"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyl hydrolase 5 (cellulase A) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.78" evidence="4"/>
    <dbReference type="EMBL" id="JAMZMK010009232">
        <property type="protein sequence ID" value="KAI7736642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C7S3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016985">
        <property type="term" value="F:mannan endo-1,4-beta-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000012">
        <property type="entry name" value="Mannan endo-1,4-beta-mannosidase 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001547">
        <property type="entry name" value="Glyco_hydro_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045053">
        <property type="entry name" value="MAN-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31451">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31451:SF64">
        <property type="entry name" value="MANNAN ENDO-1,4-BETA-MANNOSIDASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26410">
        <property type="entry name" value="GH5_mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041938878" description="mannan endo-1,4-beta-mannosidase" evidence="8">
        <location>
            <begin position="22"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 5" evidence="9">
        <location>
            <begin position="36"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF26410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7736642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="437" mass="49495" checksum="26C20483F526CD9C" modified="2024-05-29" version="1">MKKHLALSILLVALLIQNHNTIFLETEATRLTNGGFITTRGVHFMLNGSPYYANGFNAYWLMILASDPSQRSKVTAAFHDASSHGLSVARTWAFSDGGVTPLQYSPGSYNEQMFKGLDFVVAEARRFGIKLILSMVNNYENMGGKKQYVNWARSQGQYLTSDDDFFTNPVAKGFYKNHVKTVLNRYNTITGVVYKNDPTIMAWELMNEPRCTSDTSGRTIQAWITEMAAHVKSIDTNHLLEAGLEGFYSRTDRQNPGFNVGTDFISNNQISGIDFATLHSYPDQWLTSYDDQTQLNFLNNWIRTHISDAQNVLKKPLLLTEFGKSVKDPNFSPYKRDQLYNLVYNNIYLSAKHGGAAAGGLFWQLLAEGMDSFGDGYDVVLSRETSTANIIGQQGHRLYQIRKIFARMRNVQRWKQARAARRAQWLAWKRGVKPIGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C8T4</accession>
    <name>A0AAD5C8T4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">AP-5 complex subunit zeta-1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_007970</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009333">
        <property type="protein sequence ID" value="KAI7736189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C8T4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055450">
        <property type="entry name" value="AP5Z1_ARM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056856">
        <property type="entry name" value="TPR_AP5Z1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47885">
        <property type="entry name" value="AP-5 COMPLEX SUBUNIT ZETA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47885:SF1">
        <property type="entry name" value="AP-5 COMPLEX SUBUNIT ZETA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14764">
        <property type="entry name" value="SPG48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25154">
        <property type="entry name" value="TPR_AP5Z1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041922210" description="AP-5 complex subunit zeta-1" evidence="1">
        <location>
            <begin position="35"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="domain" description="AP-5 complex subunit zeta-1 ARM repeats" evidence="2">
        <location>
            <begin position="59"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="AP-5 complex subunit zeta-1 C-terminal TPR" evidence="3">
        <location>
            <begin position="308"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7736189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="594" mass="65157" checksum="D59BDAD8FE474E90" modified="2024-05-29" version="1" fragment="single">PSSSLPVQPLTLFISVSLFLSTLILQITQSTTMAEDWEIHLRTLSSSARDSNFSDDPASDASLLHSVRKLIQLCKSENSENLIARVYPQLNKIFQRSVSSISQSRSSSGLLLLAILQFFVDYGEFVLHDADPSLRTFFRSCLSREFADPVVAEATLDFLNLNKRKILLTFPTLLPQFYPLMLKLIAWNGEKLEKPFLKVFPGLMSTGSFLPLFISLVDLPVLTGALEKVEKSSGPLIGSSIATIQKSAAPEMLLALMDEAYTGSTIADGGADSESEDSSSITSDPLFLELLKDENDGLAQRHWTSPGLEAALQAAISAPSDRLKQALKMAPRLLDVYFSIALHDVNQSLKCALIPMLLDRNSKLFPDKIFSYEVHKRLLNFILAAFHQSPEFVAILKKPIVDRLGEAYDSPEKTELALQLCWAIGEHGGGGASHKDAARELFESLELLLYENLSSSRLGVGDSVLGSSSSTVRRSSQSRLLCFVVTAIAKIATHHRELLPRARVSLGKVAHSRISDARVWKRARDYLGLMNEPAICLSVLGPSVPSSGSIQKPGTINWSEGKTKIIANIPFYILGGQEGPPHHDFSLMDILPAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C958</accession>
    <name>A0AAD5C958_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Intermembrane lipid transfer protein VPS13-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_001543</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009080">
        <property type="protein sequence ID" value="KAI7737220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C958"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056748">
        <property type="entry name" value="VPS13-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45523:SF6">
        <property type="entry name" value="OS02G0470400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45523">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT (TPR)-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25037">
        <property type="entry name" value="VPS13_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Intermembrane lipid transfer protein VPS13-like C-terminal" evidence="2">
        <location>
            <begin position="316"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="482"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="517"/>
            <end position="539"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7737220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="539" mass="59655" checksum="E226C680BBB98063" modified="2024-05-29" version="1">MRGIQVDNQLPLTTMPVLFRPQRIGIEEDYLLKFLMTKQSDGSLDFCVYPYIGLQGPENSAFLISIHEPIIWRLHGMIQQIDFSRFSDAQTTAVSVDPIIEIGVLNISEFRFKFSMAMSPTQRPVGVLGFWSSLMTALGNTENMPVRINQRSLENLSMRQSVLTSNAISNIKKDVLSQPLQLLSGVDILGNASSAFGHMSKGVAALSMDKKFIQSRQRQENKGVEDFGDVIREGGGALAKGLFRGVMGIVTKPLEGAKASGVEGFVSGVGKGIIGAAAQPVSGVLDLLSKTTEGANAMRMKIAAAITSEDLLLRRRLPRVISGDNLLRPYDDYRAQGQVILQLAESGSFFLQVDLFKVRGKFALSDAYEDHFSLRKDKILLVTHRRVILLQTSNIITQKKFNPARDPCLVLWDVLWEDLATMELTTGKKDHPNAPPSKLILYLKSKSSSENNDHTRVIKCYRDTKQAFQVYTSIEQAMNTYSPKGSKEMHKRKVKRPYSATSDISTAEAVFLKERPWTSQQVSAPLPPNSTFGSSSGNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CAJ2</accession>
    <name>A0AAD5CAJ2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_014438</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the histone deacetylase HD2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008838">
        <property type="protein sequence ID" value="KAI7738272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CAJ2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.340:FF:000004">
        <property type="entry name" value="Histone deacetylase HDT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.340">
        <property type="entry name" value="Nucleoplasmin core domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041232">
        <property type="entry name" value="NPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17800">
        <property type="entry name" value="NPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="329"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="216"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="237"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="307"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="343"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7738272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="352" mass="39135" checksum="419FD334C7CCB054" modified="2024-05-29" version="1" fragment="single">AYHHKQPPPQSPSFSPTITSNHRTHDPPATTFHTIAATNVLLPTQPPSPTQYHLPPHTTYHCRRHLERPTVVCDAEYHVSIPLPLYISKTLTFYYYYFLQILCRSSSLSNLRQTMEFWGVEVKSGQPLNVILGEDKVIHLSQANLGEVKNNKPSEPVYLHINIEGKKLVLGTLNSEILPQQIFDLVIDKDFQLSHNWKNGSVYFYGYKADQPYSSDEDDIPLPVAKGKQVVKKDDNPVGDDDSSDSEDESSDEEEETPQKQQSGKKRPNESAVKTPASDKKAKLTTPQKTDGKKAGGHVATPHPSKQAKTPANKSNQKSPASASTDGAHSCKPCNRNFKSDGALQSHNQAKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CAP0</accession>
    <name>A0AAD5CAP0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">M8C21_024862</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">AA19_3_7</strain>
            <tissue evidence="21">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the leguminous lectin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008808">
        <property type="protein sequence ID" value="KAI7738483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CAP0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002229">
        <property type="term" value="P:defense response to oomycetes"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000168">
        <property type="entry name" value="L-type lectin-domain containing receptor kinase IX.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000240">
        <property type="entry name" value="Lectin-domain containing receptor kinase A4.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050528">
        <property type="entry name" value="L-type_Lectin-RKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27007">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 17 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="16" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 17 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="18" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="26"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="20">
        <location>
            <begin position="98"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="15">
        <location>
            <position position="222"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="240"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000615-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR000615-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAI7738483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="319" mass="36049" checksum="DEDA06BF17141D7F" modified="2024-05-29" version="1">MNLGPNPAKLGPSPQPTEEKNNKVRLIVGILVGTLVTFIFLFMFSFVLWRRKEKKKKEAEEHEAEESSFEVEMNNKFEKGSGPKRFSYQELAHCTSDFAEAEKLGEGGFGGVYKGFLKDLDTYIAVKRVSKSSKQGMKEYASEVSIISQLRHRNLVQLIGWCHKKGEFLLVYEYMENGSLDSHLFKGKSLLTWGKRYKILHGLASALVYLHEEWEQCVLHRDIKSSNVMLDSNFNAKLGDFGLAKLVDHEKGSQTTVLAGTLGYMAPECLVTGRATKESDVFSFGVVALEIACGRKPIEDKAQENQVRLLEWVWDLLIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CAP2</accession>
    <name>A0AAD5CAP2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_020748</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008963">
        <property type="protein sequence ID" value="KAI7737735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CAP2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7737735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="86" mass="9564" checksum="6E33512621131AEA" modified="2024-05-29" version="1" fragment="single">KKKRTLPDALPNQNCSPRLPLYSSTALPQHIILFLCLSKNTTATQYHHYLPLPVMRLSDHGTWFGPCLGSSNRLSSSDVGEIVDKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CBY3</accession>
    <name>A0AAD5CBY3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_027281</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008666">
        <property type="protein sequence ID" value="KAI7739173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CBY3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48213:SF1">
        <property type="entry name" value="PROSTATIC SPERMINE-BINDING-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48213">
        <property type="entry name" value="VID27-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="118"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7739173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="190" mass="21403" checksum="953AC2A4D8A63662" modified="2024-05-29" version="1" fragment="single">MANKSMVMASSLTGEAFESVYVDFSDSDSDSWQVIDPSDSDDDNFSYGDVTTTTTTDDDDDEDDVVVPDVDDLLRQPFGSLSSDVSMQSVVDEITLHQQEVKLAYEIAHIRQIIDNGGSINGEDAYESDEDEDNADDDLDLDDELVPKWLNNKFERQRMRKLGKRAYPKMKKSKRLACMYNKPGCVHGKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CCT3</accession>
    <name>A0AAD5CCT3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 20">Pyruvate kinase</fullName>
            <ecNumber evidence="6 20">2.7.1.40</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">M8C21_013926</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="24">AA19_3_7</strain>
            <tissue evidence="24">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>pyruvate + ATP = phosphoenolpyruvate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18157"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.40"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 20">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Plastid</location>
            <location evidence="3">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 20">Belongs to the pyruvate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.40" evidence="6 20"/>
    <dbReference type="EMBL" id="JAMZMK010008613">
        <property type="protein sequence ID" value="KAI7739517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009570">
        <property type="term" value="C:chloroplast stroma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030955">
        <property type="term" value="F:potassium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004743">
        <property type="term" value="F:pyruvate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.33.10:FF:000003">
        <property type="entry name" value="Pyruvate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.60:FF:000025">
        <property type="entry name" value="Pyruvate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="entry name" value="Phosphoenolpyruvate-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.33.10">
        <property type="entry name" value="PK beta-barrel domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1380.20">
        <property type="entry name" value="Pyruvate kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001697">
        <property type="entry name" value="Pyr_Knase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011037">
        <property type="entry name" value="Pyrv_Knase-like_insert_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018209">
        <property type="entry name" value="Pyrv_Knase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015793">
        <property type="entry name" value="Pyrv_Knase_brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015795">
        <property type="entry name" value="Pyrv_Knase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036918">
        <property type="entry name" value="Pyrv_Knase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015806">
        <property type="entry name" value="Pyrv_Knase_insert_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004491">
        <property type="entry name" value="PRK05826.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01064">
        <property type="entry name" value="pyruv_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11817">
        <property type="entry name" value="PYRUVATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00224">
        <property type="entry name" value="PK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02887">
        <property type="entry name" value="PK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01050">
        <property type="entry name" value="PYRUVTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50800">
        <property type="entry name" value="PK beta-barrel domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52935">
        <property type="entry name" value="PK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00110">
        <property type="entry name" value="PYRUVATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="17 20" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="12 20" id="KW-0418">Kinase</keyword>
    <keyword evidence="14 20" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0934">Plastid</keyword>
    <keyword evidence="16" id="KW-0630">Potassium</keyword>
    <keyword evidence="18" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 20" id="KW-0808">Transferase</keyword>
    <keyword evidence="15" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Pyruvate kinase barrel" evidence="22">
        <location>
            <begin position="99"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate kinase C-terminal" evidence="23">
        <location>
            <begin position="457"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="76"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU000504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF00224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF02887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="KAI7739517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="570" mass="62926" checksum="6B2E0A470ABC1627" modified="2024-05-29" version="1">MATINLRTHAISPSFKSDTDRRLSSAKNLNASSPSVHEFVYSNPQQQQRVRKSVSSIAVKSLKIVSQSVEPVLFSPNGPVSSSSDSSLRQPQISPDAQRKTKIVCTIGPSTSSREMIWKLAETGMNVARLNMSHGDHASHKKTVDLVKEYNAQFNEKVISIMLDTKGPEVRSGDVPKPIFLQEGQEFNFTIERGVSTNDTVSVNYDDFINDVEPGDILLVDGGMMSLEVKSKTKDLVKCEVIDGGELKSRRHLNVRGKSATLPSITDKDWEDIKFGVDNQVDFYAVSFVKNAEVVRELKDYLKSCNADIHVIVKIESADSIVNLPSILAASDGAMVARGDLGAELPIEEVPLLQEDIIRRCQNLQKPVIVATNMLESMIGHPTPTRAEVSDIAIAVRQGADAIMXXXXLPMASKFPLKAVKVMHTVALRTESSSISMVPLARKTAKKSHMGEIFGYHAATIANTLSTPIIVFTRTGSMAVILSHYRPFSTIFAFTNVKRVQQRLMLYHGVMPIYMEFSDDAEETFSRALNIIVGKSLVKEGQYVTLVQSGAQPIWRKESTHHIQVRMVQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CDX6</accession>
    <name>A0AAD5CDX6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_025493</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008575">
        <property type="protein sequence ID" value="KAI7739718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CDX6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7739718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="27" mass="3118" checksum="92C6D23A1991E601" modified="2024-05-29" version="1">MGAAEIVLLMQRRRRWWGCTLSANGQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CE52</accession>
    <name>A0AAD5CE52_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_007338</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. KIN-12 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008692">
        <property type="protein sequence ID" value="KAI7738949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CE52"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044986">
        <property type="entry name" value="KIF15/KIN-12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37739:SF16">
        <property type="entry name" value="KINESIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37739">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIN-12D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 8" id="KW-0493">Microtubule</keyword>
    <keyword evidence="5 7 8" id="KW-0505">Motor protein</keyword>
    <keyword evidence="2 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="10">
        <location>
            <begin position="101"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="25"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="45"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="176"/>
            <end position="183"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7738949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="426" mass="46410" checksum="08DC44D52CF71FD8" modified="2024-05-29" version="1" fragment="single">TKQSRNQILREIPVDTPPSNPSPIKHTKSSSRKHQHKFSKENDSPYDVPDSSPSPSVATKSSPAATKLKSPLPPRPPPSSSLKRKLSNDFSLDNCVSGSDDTGVIVRVRPTSKNEEEGEVIVQKTSGDSLTILGQPFTFDSVADATSSQVDIFNLVGAPLVENCLAGFNSSVFAYGQTGSGKTYTVWGPANALLENELSSDQQGLTPRVFERLFARISEEQSKHADKQLIYQCRCSFLEIYNEQITDLLDPAQRNLQIREDIKTGVYVENLTEESVCSIKDVTQLLKKGLSNRRTGATSINMESSRSHSVFTCVVESRCKSMDGLSSLKTSRINLVDLAGSERQKLTGAAGERLKEAGNINRSLSQLGNLINILAEVSQSGKQRHIPYRDSKLTFLLQESLGGNAKLAMVCAVSPAQSCKSETLST</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CED4</accession>
    <name>A0AAD5CED4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Oligopeptide transporter 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_022124</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oligopeptide OPT transporter (TC 2.A.67.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008527">
        <property type="protein sequence ID" value="KAI7740062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CED4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035673">
        <property type="term" value="F:oligopeptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004648">
        <property type="entry name" value="Oligpept_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004813">
        <property type="entry name" value="OPT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00727">
        <property type="entry name" value="ISP4_OPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00728">
        <property type="entry name" value="OPT_sfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22601">
        <property type="entry name" value="ISP4 LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03169">
        <property type="entry name" value="OPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0571">Peptide transport</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="46"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="110"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="182"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="252"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="313"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="339"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="425"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="498"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="523"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="546"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="575"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="682"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7740062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="682" mass="76329" checksum="CE214ACC686A7940" modified="2024-05-29" version="1" fragment="single">FNNDGSTSHAPLPAREENSPIEQVALTVPVTDDPISAVKIFYGKTLTFWVALTVVVTTQVLGFGWAGIFRRYLVEPAAMWWPQNLVQVSLFRALHEKEPRPKGGLTRNEFFLIVFICSFAYYVLPGYLFPMLTSLSWICWIFPRSVLAQQIGSGLHGLGVGAIGFDWSSISSYLGSPLASPWFATANIAVGYFLVTYVITPLAYWFNVYEAKRFPIFSDDLFTFTGQSYNTSAIVDSNFHFDSDAYDQEGQIYLSIFFAMNYAVSFACLTATVVHVFVFHGRDLWQLSKSAFQEKKMDVHSKLMRRYKQVPEWWFFCILLTNIVATLFVIEYFKSQLQLPWWAFLLACGLAFFFTLPIGVITATTNQTPGLNVITEYIIGYLYPGYPVANMCFKVYGYISVKQGIVFLQDFKLGHYMKIPPRSMFMAQVCGTLISAFAHLGTAWWLMDTVPNICDRALLPPGSPWTCPGDHVFYDASVIWGLIGPRRIFGDLGYYNSINWFFLAGALAPIIVWLLHKAFPSQTWITLITTPVLLGATINMPPATSVNYNSWIIIGFLSGFVAYRYHHNWWGRHNYVMSGALDAGLAFMGVLLYVSLDMEGVSLDWWGSNPDGCSLASCPAAAGDELLRDKWSILYNLARDKSCSIASRLARLNPGAGADWGGGVLTRVMPGLGLNGQLVVVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CFK6</accession>
    <name>A0AAD5CFK6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ternary complex factor MIP1 leucine-zipper domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_001186</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008349">
        <property type="protein sequence ID" value="KAI7740753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CFK6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006869">
        <property type="entry name" value="DUF547"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025757">
        <property type="entry name" value="MIP1_Leuzipper"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46248">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46248:SF12">
        <property type="entry name" value="TERNARY COMPLEX FACTOR MIP1 LEUCINE-ZIPPER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04784">
        <property type="entry name" value="DUF547"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14389">
        <property type="entry name" value="Lzipper-MIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ternary complex factor MIP1 leucine-zipper" evidence="4">
        <location>
            <begin position="25"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="DUF547" evidence="3">
        <location>
            <begin position="349"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="192"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="28"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="114"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="210"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7740753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="423" mass="47255" checksum="711113A323A0AA49" modified="2024-05-29" version="1">MEIPRCKSKSKTTGKPLLNRRLTRSERKLALLEDVDKLKKKLRQEENVHRALERAFSRRLGTLPHLPPYLPPQTLELLAEVAVLEEEVVRLEEEVVSFRHGLYQEAVCLSSGISNNSDNNAVDQSAKSGDGSRLSMSPVEIHLETSKLAQDSEQKPLNSLLRSASSRISYSNRIGTDFMNRVVEKKPTADLKKSDLGISGDGVLGKENRSSANSNSKVKNSPERVAEEMQSLVKRPQIKFETAGKCSPPKLQNRLADQEKAQESCSTKTMDAEAFSNLMSLDLTGGDRGASFRDPYGVCLKSKRRDIGPYKDLFAIEAGTIDFKRKMNASLLMRRLKLLLEKLASVNLEGLTHQQKLAFWINTYNICMMNAYLEHGTPESPEMMPTLMQKATINAGGYLLNAITIEHSILRLPNRLKPVSCNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CJD7</accession>
    <name>A0AAD5CJD7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_020709</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007930">
        <property type="protein sequence ID" value="KAI7742702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CJD7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31818">
        <property type="entry name" value="O-FUCOSYLTRANSFERASE 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31818:SF3">
        <property type="entry name" value="O-FUCOSYLTRANSFERASE 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7742702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="148" mass="16766" checksum="DE4EAF0D4DC49C14" modified="2024-05-29" version="1">MGELKGWRYSGGTLVVANNNNNQRKKVKYNQQYYYRHCCCSWSVVCGVGLFVLGLISLFTGHVASNLEWYSQKLAKHRLWYYKVGGFGHGPIDIWKSEFSKLYYGCSERGPHYAPPVHESLSNGYLLIAASGGLNQQRTGVNFVFCPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CM06</accession>
    <name>A0AAD5CM06_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">GOLD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_030347</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Endomembrane system</location>
            <topology evidence="7">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 8">Membrane</location>
            <topology evidence="1 8">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the EMP24/GP25L family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007584">
        <property type="protein sequence ID" value="KAI7744097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CM06"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015720">
        <property type="entry name" value="Emp24-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036598">
        <property type="entry name" value="GOLD_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22811">
        <property type="entry name" value="TRANSMEMBRANE EMP24 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01105">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01190">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101576">
        <property type="entry name" value="Supernatant protein factor (SPF), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50866">
        <property type="entry name" value="GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="GOLD" evidence="9">
        <location>
            <begin position="8"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7744097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="187" mass="21836" checksum="1E90699D6654DD3A" modified="2024-05-29" version="1" fragment="single">IRFVIDREECLSHNVEYEGDTVHVSFVVIKSETMWHSTDDGVDLLVTGPGNEHVHALHDRTSEKYEFMAREKGVYHFCFKNKSPYHETIDFDVQVAHFAYHDQHAQDEHFHPLFEQISKLEEALYNIQFEQHWLEAETDRQAVVNEGMSKRAVHKAIIESIALVGVSILQVYLLQRLFERKLGTSRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CN78</accession>
    <name>A0AAD5CN78_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ethylene-overproduction protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_013553</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007370">
        <property type="protein sequence ID" value="KAI7745081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CN78"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010105">
        <property type="term" value="P:negative regulation of ethylene-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044631">
        <property type="entry name" value="ETO1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44203:SF3">
        <property type="entry name" value="ETO1-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44203">
        <property type="entry name" value="ETO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13176">
        <property type="entry name" value="TPR_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="738"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="783"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="919"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7745081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="919" mass="104532" checksum="18F7C147C2C05216" modified="2024-05-29" version="1" fragment="single">CKKPTQVHALTINSILSSSSHSDDSLSFLNVNNNNNNINVNVKSLHLPYGLPDLDFIVPKIESFLYHVDFIEKSCDLYKRIAVSCCGDEYGVCGLYLEQYALLCALGDGKLLRRSLQLAREYAVDVHSKVVLSAWVRYERREDELVGVSPLDCIGKVLECPKAALVDDGRACFRSVFDRCQCVFESVIDLSCNDDEVETETETETETVCFCIGNEVISCVRGKIAALSSPLNTLLYGSFVESVKEKIDFSQVGISVEAMRGVEAFSRTKKLMDFDSPSVVLEMLTFSQKFCCEEMKSACEVYLSSLVSNVHDALIFIEYGFEDNASLLVASCLQVILRELPGSLNNPKVLSLFCSHESMEKLSMVNHASFLLYYFLTQVAMEEKMTASNAVMLVERLLELATEKWQKTLAFHQLGVLLLEKEEYKSAQSCFEAAYEMGHVYSATGIARSMYKQGKTYLARDLINKIISDCKRPTGWMYQERSLYNLGSKKIQDLELASKLDPTLAYPYKYRAVSMVTENLINEAIQEINKIIRFKVSPDCLELRAWFYLVLEDYQAAVRDIRALLSLEPYYMMFHGKMRCDHLVDLLSQRVKEWTPADCWLQLYDRWSCIDDIGSLAIIHQMMAHDPLKSLLRFRQSLLLLRLNCQKAAMRSLRLARNLATAKHERLVYEGWILYDTGYREEALSKADESISIERSFEAYFLKAYTLADGSLDPDSSAVVIQLLVDALKCPSDGLRKGQALNNLGSIYVDCGKHDLAADCYINALDIKHTRAHQGLARVYSLKDDRKAAYDEMTKLIEKAENNASAHEKRSEYCDRDNAVNDLYVATQLDPLRTYPYRYRAAVLMDEQKEAEAVEELTKAIAYKPELQMLHLRAAFYESMSHYDLALRDCEAALCLDPNHKETLELYNRTLKESAEFYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CQF4</accession>
    <name>A0AAD5CQF4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_007943</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007110">
        <property type="protein sequence ID" value="KAI7745837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CQF4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7745837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="130" mass="14523" checksum="4EE14FC60710F466" modified="2024-05-29" version="1">MSSPYNQSLKVLQINTKEAAMTTKKAQRLLDASKSTHDNSVTSNGENQMHRVSYLLTLIGAELASQEERSAASLAAYIALAEELTEMGLFSKKLKLLDLVNCNKELKMVFVAKCEELEEHVVYLKDDRSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CR64</accession>
    <name>A0AAD5CR64_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Lipoxygenase</fullName>
            <ecNumber evidence="14">1.13.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">M8C21_003293</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">AA19_3_7</strain>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Plant lipoxygenase may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="14">Lipid metabolism; oxylipin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the lipoxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.13.11.-" evidence="14"/>
    <dbReference type="EMBL" id="JAMZMK010006962">
        <property type="protein sequence ID" value="KAI7746477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CR64"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016702">
        <property type="term" value="F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034440">
        <property type="term" value="P:lipid oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031408">
        <property type="term" value="P:oxylipin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.245.10:FF:000002">
        <property type="entry name" value="Lipoxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.60:FF:000002">
        <property type="entry name" value="Lipoxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.375.10:FF:000001">
        <property type="entry name" value="Lipoxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.375.10">
        <property type="entry name" value="Lipoxygenase-1, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.372.10">
        <property type="entry name" value="Lipoxygenase-1, Domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.245.10">
        <property type="entry name" value="Lipoxygenase-1, Domain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.60.20">
        <property type="entry name" value="PLAT/LH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000907">
        <property type="entry name" value="LipOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013819">
        <property type="entry name" value="LipOase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036226">
        <property type="entry name" value="LipOase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020834">
        <property type="entry name" value="LipOase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020833">
        <property type="entry name" value="LipOase_Fe_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001246">
        <property type="entry name" value="LipOase_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027433">
        <property type="entry name" value="Lipoxygenase_dom_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001024">
        <property type="entry name" value="PLAT/LH2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036392">
        <property type="entry name" value="PLAT/LH2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11771">
        <property type="entry name" value="LIPOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00305">
        <property type="entry name" value="Lipoxygenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01477">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00087">
        <property type="entry name" value="LIPOXYGENASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00468">
        <property type="entry name" value="PLTLPOXGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00308">
        <property type="entry name" value="LH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49723">
        <property type="entry name" value="Lipase/lipooxygenase domain (PLAT/LH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48484">
        <property type="entry name" value="Lipoxigenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00711">
        <property type="entry name" value="LIPOXYGENASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00081">
        <property type="entry name" value="LIPOXYGENASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51393">
        <property type="entry name" value="LIPOXYGENASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50095">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 13" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="11 14" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="9 13" id="KW-0408">Iron</keyword>
    <keyword evidence="3 14" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5 14" id="KW-0925">Oxylipin biosynthesis</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PLAT" evidence="16">
        <location>
            <begin position="37"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="Lipoxygenase" evidence="17">
        <location>
            <begin position="160"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="825"/>
            <end position="852"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAI7746477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="881" mass="100672" checksum="E79FF538A57F4A67" modified="2024-05-29" version="1">MADVDRRKRMKIEDSKIKGTLVLTNHHSVSSLKDEFFGRRVLLQLVSAFPSESTVIILAIEGRGVRGKLGKETHLNWLNTFITRSTKGDSTFELDFDPDDDIGVPGAFLIINRHHKEFYLKTLTLTNVPNVGRLHFVCNSWVYPEEHYTKRYRVFFSNQTYLPGETPEGLRHYREEELDELRGDGTGKREEWDRVYDYDVYNDLSQPEKGSELVRPIFGGSIDHPYPRRCRTGRPLSDTDPRMESRIGILHSLEIYVPKDERFSDLKMADVYAYGLKLVSQGLLPGFESVWDKISDEIVSGLEHILNHKFEGVSSNKFNEFSSFKDVLNLYEGGLPVPKSNVLEGLRAKIPSEFIRELLRSDGEHLSKFPLPKVIEVDRSAWRSDEEFGREMLAGICPVVIRRLQKFPVTSELDPVKFGNQNSRITEDHIRDQMDGFSVHEAIKNNRLFILDHHDPLMPYLRGINETGTKTYATRTILFLQNNSTLKPIAIELSLPHPNGEEAGVISTVHTPASHGAKGTIWLLAKAYANVNDSGYHQLVCHWLHTHASIEPFIIATNRQLSVLHPIHKLLHPHFRDTMNINALSRQTLINAGGLLEKTVFPDKYSLAMSCNMYKKWDFTKQALPADLIDRGMAVEDPNSPHGVRLVIEDYPFAVDGLEIWSAIKSWVQDYVFIYYKNDEDIQNDNELKNWWDEVRTKGHGDLKDADWWPKMHTREELIQSCTIIIWVASALHAAVNFGQYPYGGYLPNRPAMSRRLLPEPGTPDYEELETDPEKAFLKTVTPQLQSILGISLIEVLSRHSADEVYLGERDTPEWTADKQALEAFARFGSNLERIENKIEEMNLDKKLLNRTGPAQMPYTLLYPRSDIGLTGRGIPNSVSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CRF3</accession>
    <name>A0AAD5CRF3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_007281</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007093">
        <property type="protein sequence ID" value="KAI7745860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CRF3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7745860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="95" mass="10634" checksum="05E1B1526CED5D07" modified="2024-05-29" version="1" fragment="single">YLIGSSSLPTNYGSGFLSFCKSFLTGVFSCCGSDLTDPQRGRRPVSLPTASKWSWWSTMRRFQSSVCYQCQRHSKFLNGIRPGVLNLLGNQNFVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CRP7</accession>
    <name>A0AAD5CRP7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_005350</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007054">
        <property type="protein sequence ID" value="KAI7746010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CRP7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33735">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33735:SF14">
        <property type="entry name" value="PHAGE CAPSID SCAFFOLDING PROTEIN (GPO) SERINE PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="179"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7746010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="216" mass="23964" checksum="082B4A83BA712A5F" modified="2024-05-29" version="1">MAATLFMSCGLNNINKLSNQRFDPLSASFSTTATTWLPLSKGGMFLPPSNGSYLSSGNWASKSKRKFVVFSADQVADLLPIGMDHLPQDWPAWLPGVLLALVVPFFTYKWGPFGKFKAELDKVEEAVDNVADRVEEIAEKVEEFVGDIADDLPEGSQFRQTLEKVEMVADTIGKDAKMVSDMVDKMDEMEAKLETMLDKAAEKEKKTTTPTKLDQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CS83</accession>
    <name>A0AAD5CS83_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Vacuolar protein sorting-associated protein 55-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_013753</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the OB-RGRP/VPS55 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006918">
        <property type="protein sequence ID" value="KAI7746692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CS83"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032511">
        <property type="term" value="P:late endosome to vacuole transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007262">
        <property type="entry name" value="Vps55/LEPROT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12050">
        <property type="entry name" value="LEPTIN RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12050:SF0">
        <property type="entry name" value="RH04491P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04133">
        <property type="entry name" value="Vps55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="65"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7746692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="137" mass="15159" checksum="B53A0E74FE872A4A" modified="2024-05-29" version="1">MLSALMYILVPMPCMFFGDGSTESLINRDDDGCRFCLGGLEQLPFHSIRLVPGLLRQSPRVGGGWINAAKFLTGASLVGSLAIPIILRHADFISTGAMLIEFLSFFIFLGTVMCYYRVNSEDEWSTKESSLGMFFTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CUT9</accession>
    <name>A0AAD5CUT9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_010986</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006561">
        <property type="protein sequence ID" value="KAI7748319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36053:SF1">
        <property type="entry name" value="OS04G0680300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36053">
        <property type="entry name" value="OSJNBB0017I01.18 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="149"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="169"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7748319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="186" mass="20054" checksum="B270F62D1EDC62BD" modified="2024-05-29" version="1">MTGSEPRTVAVGSVFTFDPPAIRSVVEEISVTVGDLTEGRGLEAGKPKIGREMIPQQWTPPCGNQCTHKYAALMQIPWRVFCKKGCNADGDTLEECVSECDEICYKDPVLKXXXXYIDRSPGSASYSQDCFHACVSGCGYKFDIPPEKVTGIHPNRPSPPPPAAAKSDPAVDKSKKVSEDVPCTSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CUU7</accession>
    <name>A0AAD5CUU7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_028531</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006614">
        <property type="protein sequence ID" value="KAI7748027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CUU7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11393">
        <property type="entry name" value="bHLH_AtbHLH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000075">
        <property type="entry name" value="Transcription factor bHLH113 family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045239">
        <property type="entry name" value="bHLH95_bHLH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045843">
        <property type="entry name" value="IND-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16223:SF249">
        <property type="entry name" value="TRANSCRIPTION FACTOR BHLH154"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16223">
        <property type="entry name" value="TRANSCRIPTION FACTOR BHLH83-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="7">
        <location>
            <begin position="23"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="10"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7748027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="100" mass="10974" checksum="955D0232AC7156C1" modified="2024-05-29" version="1">MMEHKRSPVSIEQGSLTSLTPKKHKAGLSISAKERKANFGDKIAALQKLVSPYGKTDTASVLHEAMEYINFLHDQVKVLSAPYLLQSKPTALSQVRAITP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CV27</accession>
    <name>A0AAD5CV27_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Formin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_002330</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the formin-like family. Class-I subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006763">
        <property type="protein sequence ID" value="KAI7747409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CV27"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045010">
        <property type="term" value="P:actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2220">
        <property type="entry name" value="Formin, FH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015425">
        <property type="entry name" value="FH2_Formin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042201">
        <property type="entry name" value="FH2_Formin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027643">
        <property type="entry name" value="Formin-like_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23213:SF359">
        <property type="entry name" value="FORMIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23213">
        <property type="entry name" value="FORMIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02181">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00498">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51444">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="109"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="FH2" evidence="5">
        <location>
            <begin position="316"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="149"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="199"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="744"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="149"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="246"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="292"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00025793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU361260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7747409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="771" mass="85419" checksum="A686BB26084E16E8" modified="2024-05-29" version="1">MDNDDDMAELLSMNCETELASCMEDFQDVEYCDSDKTLMGKERMLKSINVQDRDVKKTVQDCLRPKRVALSKSRKERLNKKWYIEYLVSFYNKRSDANGRRRLDESDSLETVGVVVIVTAIVTLCLASLLYYCYVRKFGLAGIQSDERPLPRLSTDKDSTSGALKKLLKPKSKYRNTEDQFNGQMDASVAVDTGVHMKASLDPSVHPPAESVNASTQHTLKPRAVRAKSSSAGHAGSPVGGKESSHLQPQANGGSHVSSPATAPTASNPETSCAAHNAPETSSDKPLHEVVPSPPPPPPPKPGGNAGASIRPRRHADPAHAPKPKLKPFFWDKVTASPDQQMVWDKIKSGSFQFDEEMIERLFGYEDAVKNKDQDIKNTAKEPQIHFIQIINPRKAQNLSILLKALNVTAEEVSVALIEGNELPIEIVQTLLKMAPSSEEELSLRLYGGDLSRLGSAERFLKKLVEIPYAFKRLESLLFMCTLQDEEANIKESFETLEAACVELRKSRLFLKLLEAVLKTGNRMNVGTFRGSATAFKLDTLLKLSDVKGVDGKTTLLHFVVQEIIRGEGIRVARAAKAGNKNICPIKTGDLLLDPSKEEEADEHYCKLGLEVVSGLSSELENVTLAAMIDAERLTSSVSKLAYDLLKARESLNTDKKNLEGQSEDAEPDEFWLILSAFLETAEKEISWMVEEEKRIMGLVKNTVDYFHGNAGKDEGVRLFSVVRSFLIMLDKVCKEIQAAPIKPLKKKDDQSQPTSSDPMLRAFRSPPNAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CVE4</accession>
    <name>A0AAD5CVE4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PRC-barrel-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_029970</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006480">
        <property type="protein sequence ID" value="KAI7748712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CVE4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011033">
        <property type="entry name" value="PRC_barrel-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36740">
        <property type="entry name" value="PRC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36740:SF1">
        <property type="entry name" value="PRC-BARREL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50346">
        <property type="entry name" value="PRC-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="291"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="294"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="316"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7748712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="327" mass="36999" checksum="27CF43FF30E60ACA" modified="2024-05-29" version="1">MCDCTSSSLSSYSCIKFPPSSIHIISLPPSFHHTSPNPPKFSIKTSQNPNPKTSQSNPNQLNFKDTQTTKSQLNLNPNDHEDPEFLQFQGDGDDLVEIEDLELKLGELDDDDDDDDCKDKVVRRKGKQLMKRSNFIAKQVITIQSALSLGFVSQLWVDTNTVGDVVLVEDETVMDKEFKLVGLETLVGYSVVTPGRRDIGKVRGYTFNINSGAIESLELDSFGISIIPSSLVSTYSLLVEDVLEVVSDTVVVHDAAASRIQRLTKGFWDAQRTNYSMDDLDYYSDVEERSEYRKGRRRRRPQPGNPRRREKPIDNIPEDWDLPMDYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CVH8</accession>
    <name>A0AAD5CVH8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein trichome birefringence-like 34</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_001604</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PC-esterase family. TBL subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006411">
        <property type="protein sequence ID" value="KAI7749078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CVH8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016413">
        <property type="term" value="F:O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029962">
        <property type="entry name" value="TBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026057">
        <property type="entry name" value="TBL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025846">
        <property type="entry name" value="TBL_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32285:SF11">
        <property type="entry name" value="PROTEIN TRICHOME BIREFRINGENCE-LIKE 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32285">
        <property type="entry name" value="PROTEIN TRICHOME BIREFRINGENCE-LIKE 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13839">
        <property type="entry name" value="PC-Esterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14416">
        <property type="entry name" value="PMR5N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Trichome birefringence-like N-terminal" evidence="8">
        <location>
            <begin position="17"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="Trichome birefringence-like C-terminal" evidence="7">
        <location>
            <begin position="71"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF14416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7749078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="354" mass="41482" checksum="CED7CB1186109613" modified="2024-05-29" version="1" fragment="single">MIQARVDVTFSGDSQSECNLFSGKWVFDNISYPLYKEKDCKFMSDQLACNRFGREDLSYQHWRWQPHHCDLPRFNATKLLQRLRNKRMVFVGDSLNRGQWVSMVCLLDSAIHNPKMKFLSYVNGSNLIAFKAREYNASIEFYWAPLLVESNSDDPVNHRLPERIVKSQSIEKHGRVWTDADILVFNSYLWWRQPHIKVLWGSFEDKNGIYKEVEMLRSYELALKTWSDWLEIHVNRTKTSLFFVSMSPTHERANEWGASSDQNCYSETGMITKEGYRGSGTDPKMMRIVENSIDELKTRGLKVEMINITQLSEYRKEGHPSIYRKQWDALSEEQLSNPSSYSDCIHWCLPGVPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CWE3</accession>
    <name>A0AAD5CWE3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_000336</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Required for the accumulation of coenzyme A in the mitochondrial matrix.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006484">
        <property type="protein sequence ID" value="KAI7748687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CWE3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015228">
        <property type="term" value="F:coenzyme A transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990559">
        <property type="term" value="P:mitochondrial coenzyme A transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000014">
        <property type="entry name" value="mitochondrial coenzyme A transporter SLC25A42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24089">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="26"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="122"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="230"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00055062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7748687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="335" mass="36342" checksum="CF5432AF3C7E45F0" modified="2024-05-29" version="1">MGSSQGSSLSTNVAGLVEGSAIDTLPVYVKELIAGGAAGAFAKTAVAPLERTKILLQTRTQGFHSLGVYQSLKRLLKHEGVPGFYKGNGASVLRIVPYAALHFMTYEQYRCWILDNYSVFGTGPVVDLLAGSAAGGTAVLCTYPLDLARTKLAYQVVDAKPSLRNGSKSTTAQPRYSGIKNVLESVYREGGMRGLYRGVGPTLIGILPYAGLKFYIYEKLKSHVSEENQRSIMMRLSCGALAGLFGQTFTYPLDVVRRQMQVENLHAGGGVRHASTWKGITTIVSEQGWRQLFAGLSINYIKIVPSVAIGFTAYDMMKSWLRIPPRQKTQSISAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CWJ5</accession>
    <name>A0AAD5CWJ5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Expansin-like EG45 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_016319</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006675">
        <property type="protein sequence ID" value="KAI7747816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CWJ5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22269">
        <property type="entry name" value="DPBB_EG45-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="entry name" value="RlpA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007112">
        <property type="entry name" value="Expansin/allergen_DPBB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009009">
        <property type="entry name" value="RlpA-like_DPBB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47480">
        <property type="entry name" value="EG45-LIKE DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47480:SF1">
        <property type="entry name" value="EG45-LIKE DOMAIN CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03330">
        <property type="entry name" value="DPBB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="Barwin-like endoglucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50842">
        <property type="entry name" value="EXPANSIN_EG45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042202046" description="Expansin-like EG45 domain-containing protein" evidence="1">
        <location>
            <begin position="28"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="Expansin-like EG45" evidence="2">
        <location>
            <begin position="41"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7747816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="141" mass="15314" checksum="151D9F4BB8EBB4CF" modified="2024-05-29" version="1">MFNLKLHLSHPWLSLLILPILFEVIHCEVGTASLYNPPYTPTACYGSDASQFPSSNLFAAAGEGIWDNGAACGRQYLVKCISAAIPRTCIPGQTIEVRIVDRAQTSVSRPTRAGTTMVLSDTAFRTIANPISSINIEFQKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CYF6</accession>
    <name>A0AAD5CYF6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_008912</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Channel protein in tonoplast. These proteins may allow the diffusion of amino acids and/or peptides from the vacuolar compartment to the cytoplasm.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the MIP/aquaporin (TC 1.A.8) family. TIP (TC 1.A.8.10) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006306">
        <property type="protein sequence ID" value="KAI7749692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CYF6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009705">
        <property type="term" value="C:plant-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015250">
        <property type="term" value="F:water channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00333">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1080.10:FF:000002">
        <property type="entry name" value="Probable aquaporin TIP1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="entry name" value="Glycerol uptake facilitator protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034294">
        <property type="entry name" value="Aquaporin_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000425">
        <property type="entry name" value="MIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022357">
        <property type="entry name" value="MIP_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00861">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45665:SF37">
        <property type="entry name" value="AQUAPORIN TIP2-3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45665">
        <property type="entry name" value="AQUAPORIN-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00230">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00783">
        <property type="entry name" value="MINTRINSICP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81338">
        <property type="entry name" value="Aquaporin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00221">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 10" id="KW-0813">Transport</keyword>
    <keyword evidence="3" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="54"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="140"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="172"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="214"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00053065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7749692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="250" mass="25235" checksum="F302682E151111B6" modified="2024-05-29" version="1">MVKLAFGDFGDSFSGTSLKSYLAEFIATLLFVFAGVGSAIAYGKLTADAALDPAGLVAIALAHGLALFVGVSMAANISGGHLNPAVTFGLAIGGNITILTGLFYWIAQLLGATVASFLFQFVTGGLAVPTHAVADGMSAIQGVVFEIIITFALVYTVYATAADPKKGSLGTIAPMAIGFIVGANILAAGPFSGGSMNPARSFGPAVASGDFSQIWIYWVGPLIGGGLAGLIYGDVFIGSYASLPASEDYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CYH6</accession>
    <name>A0AAD5CYH6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">WAT1-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_012133</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the drug/metabolite transporter (DMT) superfamily. Plant drug/metabolite exporter (P-DME) (TC 2.A.7.4) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006277">
        <property type="protein sequence ID" value="KAI7749775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CYH6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030184">
        <property type="entry name" value="WAT1-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31218">
        <property type="entry name" value="WAT1-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="18"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="50"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="79"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="107"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="192"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="258"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="284"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="7">
        <location>
            <begin position="19"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7749775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="349" mass="38472" checksum="6589EA317994E4F2" modified="2024-05-29" version="1">MEMSTGWFWCFCRRGRPYLGVLFLQLGYAVNNILVKSALNEGLNPYTFSVYRNVAAAVAFGPFALYFERKIRTRMTFSVFWKIMLLALIEPVLDQILYYTGMKYTTATFAIAMCNVLPALTFVMAWIFRLEKVNVKKLHSQGKILGTLVTVGGAMIMTLVNGPPVPLPWTKEGAEVNHALAPNPVVSQDQHIKGAIMITAGCFCWASFYILQAMTLKEYPAQLSLTTLISSGHNLLGIVRSGASYYVSGLVMKEKGPFFVTAFNPLGMVIVAIVSSFALAERLVLGRVLGALIIVGGLYLIIWGKSKDSTLASSNSSEVEICDQEKCDDKDLDDAIKASKEDRRSEAVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CYH7</accession>
    <name>A0AAD5CYH7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">M8C21_032802</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">AA19_3_7</strain>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18 22">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17 22">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010006451">
        <property type="protein sequence ID" value="KAI7748881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CYH7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000394">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000041">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000146">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase IOS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024788">
        <property type="entry name" value="Malectin-like_Carb-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45631">
        <property type="entry name" value="OS07G0107800 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45631:SF19">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12819">
        <property type="entry name" value="Malectin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="392"/>
            <end position="669"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAI7748881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="715" mass="79507" checksum="8CB28F19AF7CB0E7" modified="2024-05-29" version="1">MIIRAPSSSVDVCLCCATTGSPFLSTLELRPLNLSMYATDFEDNFYLKVAARVNFGASSTEAVRYPDDPYDRIWDSDLAKRQNFLVGVAPGTVRINTTKEINTNTREYPPMKVMQTAVVGTQGILSYRLNLEDFPANARAYAYFAEIQDLPENETRKFTMQDPYIPDYSNAVTPDSTQGPLLNAVEISKYVLISSKTEKNDVNVLGTFRSMLDNGDLTEEGDPCVPVQWEWVTCSSTSPPRITKLVLSGLNLEGEIPSGIKDLEELTELWLNGNSLNGSIPDMSNLEKLTIIHLEDNKLTGSLPSYFGSLPNLKELYIQNNSLTGEIPSELLTGKIIFKKKAPPQRSDDKGAISSRKSIIGSTAYSVARAGSLMDEGVSYYFSLAEIEEATSGFSKKIGKGSFGPVYYGKMKDGKEVAVKMMADSSSHGAQQFVTEVALLSRIHHRNLVPLIGYCEEEHHRMLVYEYMHNGTLRDHSNMQTDKVNKKHLDWRARLRIAEDSAKGLEYLHTGCNPSIIHRDIKTSNILLDINMRAKVSDFGLSRQTEEDLTHISSVARGTVGYLDPEYYANQQLTEKSDVYSFGVVLLELISGRKPVSPEAYGADWSIVHWARSLIRKGDVVSIIDPTIANNVKIESVWRIAEVAIQCVEQHGSSRPKMQEIILAIQDAIKIEKGNDGKITSESSRAQSSRKTLLTTFLDIESPDLSNECLFPSAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CYJ3</accession>
    <name>A0AAD5CYJ3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Solute carrier family 40 member</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_013023</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">May be involved in iron transport and iron homeostasis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ferroportin (FP) (TC 2.A.100) family. SLC40A subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the major facilitator superfamily. Phosphate:H(+) symporter (TC 2.A.1.9) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006093">
        <property type="protein sequence ID" value="KAI7750721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CYJ3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005381">
        <property type="term" value="F:iron ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd17480">
        <property type="entry name" value="MFS_SLC40A1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009716">
        <property type="entry name" value="Ferroportin-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11660">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 40 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11660:SF57">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 40 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06963">
        <property type="entry name" value="FPN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="60"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="93"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="128"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="182"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="209"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="302"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="334"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="370"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="445"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="241"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="251"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="263"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU365065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7750721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="486" mass="54164" checksum="341E152965E05164" modified="2024-05-29" version="1">MEDRYYVRLEDEHHHQLQHPVPTSLLVYLYIGHFLARWGARTWEFSVGLYMINVWPDSLLMAAAYGVVESATTAVFGPLVGQWIDDSTYPKVLRVWLLTQNLSFIVAGVTVVGLLISPDMRVTNATTFTLLVVLTNLSGAVAVLSTLAGTILIEREWVVVISEGHSSNLLTKLNSMIRRIDLISTLFAPVVTGFIISFVSMIASAVGLAIWNILSVFLQYWFLNSVYKGIPALQERSRKRGLKLKTKPNDQEQAPSTSQEETNQDHDSSDDVASRSLVRKFVERVSESPFIHAWQVYLQQDIVLAGLSLAVLYFTVLSFGTLMTATLEWDGIPAYIIGTGRGISAIMGISATFLYPFLETRISTVRTGLWSIWSQWTCLLICVGSIWVKNNTTSAYMLMSGVAVSRIGLWVFDLSVIQQMQDQVPESDRAVVGGVQNSVQSFWDLMTYIMGLIISNPQVILLLYIIGFLEVDLGFFRVGDDSGGYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CYJ4</accession>
    <name>A0AAD5CYJ4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_010434</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006479">
        <property type="protein sequence ID" value="KAI7748715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CYJ4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058594">
        <property type="entry name" value="PB1-like_dom_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26130">
        <property type="entry name" value="PB1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="202"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="208"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="233"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="265"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7748715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="291" mass="32641" checksum="5873C41FC6480B1A" modified="2024-05-29" version="1">MYDQEFRRLGFRVCKNLTHEEVLELYVGALSLFSIKLHHNGRFTRFPDRTYVEGEENFIDLLDHDKFSVHEMDRILEKFGYQKDIERYYHYQVPGSDLDFGLRALGSDADGIGFIKVIPESSEHGSDDDDDASEDSDFLVDEGNLINEVETNMRDFRFFTIMPLFYHKAVGRPKKVRRKLVVELDEMTAYGKLRKVGTYGTCGKCGNKGHNSRSCNDRTPDESGSSKSKKDGKKAAKKDGKKAAAAGNDGKKAAAVGTTMQGQVKPKKGGVKMCKKCKGAGHNSRTCQKNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D0W7</accession>
    <name>A0AAD5D0W7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_003785</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the leguminous lectin family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the leguminous lectin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006231">
        <property type="protein sequence ID" value="KAI7750035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D0W7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06899">
        <property type="entry name" value="lectin_legume_LecRK_Arcelin_ConA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000985">
        <property type="entry name" value="Lectin_LegA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001220">
        <property type="entry name" value="Legume_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050258">
        <property type="entry name" value="Leguminous_Lectin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32401">
        <property type="entry name" value="CONCANAVALIN A-LIKE LECTIN FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32401:SF47">
        <property type="entry name" value="LEGUME LECTIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00139">
        <property type="entry name" value="Lectin_legB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00308">
        <property type="entry name" value="LECTIN_LEGUME_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0430">Lectin</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="248"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="584"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="454"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="701"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7750035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="701" mass="77588" checksum="DB2B1ADCE941C42C" modified="2024-05-29" version="1" fragment="single">FKIDRFSADATDILYRGDAIPSVGTIEFNKVNYITRVGQAMYHDAVPIWDRKSNKLTDFTTHFTFIIDTLGRAKSYYGHGLTFFLAPVTFEIPPNSAGGFLGLFNTSYTDSSRNQMIVVEFDSFVNPEWDPPVEHVGINKNSIHSSVYTAWNASLHSGDSADAWVSYNATTQDLNVRWSYGAGNDSKGDTNTSLSYHVDLREVLPERAMVGFSAATSGTPIERHILQSWEFNSSLVHKGEDNSKEWKLAVGTAVPLGVLVLVGMAACGLYWRKRRKPAEEAVEAVALTSINDDLERGVGPRRFSYSDLVSATNNFSNDRKLGEGGFGCQAGQKKLKGVKWRMLQHSITKGNMVLDIVINYYIKGSRTVIDHFDADATNILYRGDAIPSVGTIEFNKVNYLNRVGQAIYYDTVPIWDRQSGKLTDFTTHFTFIIDTQGRSPYGNGLTFFLAPTTFQIPTNSGGGFLGLFNTTYTDSPRNQLIVIEFDSFVNPEWDPPVEHVGINKNSIHPATYAAWDASSHSGETTNAWVSYNSTTQLLSLEWSYSTGNDSRGNLSYQVDLREVLPERVMVGFSAATGNSKKRKLVVEIGVPLGVLVLIAMAASVVISSLRHRNLVRLIASALLYLHEEWEQCVLHRDIKTSNIMLDSGFNVKLGDFGLARLMNHDQLSPQTTDLAGTLGYLAPECMATGKASKESDVYSFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D298</accession>
    <name>A0AAD5D298_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_013058</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="JAMZMK010006093">
        <property type="protein sequence ID" value="KAI7750756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D298"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1080:FF:000003">
        <property type="entry name" value="Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:006066">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF109">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DHX15 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="67"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="10">
        <location>
            <begin position="202"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7750756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="642" mass="72644" checksum="8CB5B4A680E4E9FB" modified="2024-05-29" version="1">MGTERKRKVSLFDVVDDSSTAKLTKSNGVDNMSTVNRWNLRPYSQRYYDILEKRKTLPVWHQKEEFLKALRDNQTLILVGETGSGKTTQIPQFVLEAVELESADRRKKFMVGCTQPRRVAAMSVSRRVAEEMDVTIGEEVGYSIRFEDCSSAKTVLKYLTDGMLLREAMTDPLLERPDLKLVVMSATLEAEKFQGYFFAIRTVVQIHMCEPPGDILVFLTGEEEIEDACRKITKEVGNLGDQVGPVKVVPLYSTLPPAMQQKIFEPAPPPLTEGGPAGRKIVISTNIAETSLTIDGIVYVIDPGFAKQKVYNPRVRVESLLVSPISKASAHQRSGRAGRTQPGKCFRLYTEKSFNNDLQPQTYPEILRSNLANTVLTLKKLGIDDLVHFDFMDPPAPETLMRALEVLNYLGALDDEGNLTKLGEIMSEFPLDPQMGKMLVVSPEFKCSNEILSIAAMLSVPNCFVRPREAQKAADEAKARFGHIDGDHLTLLNVYHAYKQNNEDQSWCYENFVNQRALKSADNVRQQLSRIMARFNLKLCSTDFNSRDYYINIRKAMLAGYFMQVAHLERTGHYLTVKDNQVVHLHPSNCLDHKPEWVIYNEYVLTSRNFIRTVTDVRGEWLVIFSYISFLLSTSINNGPLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D451</accession>
    <name>A0AAD5D451_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dilute domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_012813</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005829">
        <property type="protein sequence ID" value="KAI7751731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D451"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002710">
        <property type="entry name" value="Dilute_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052072">
        <property type="entry name" value="Vascular_dev_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16027:SF6">
        <property type="entry name" value="DILUTE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16027">
        <property type="entry name" value="DILUTE DOMAIN-CONTAINING PROTEIN YPR089W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01843">
        <property type="entry name" value="DIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01132">
        <property type="entry name" value="DIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51126">
        <property type="entry name" value="DILUTE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dilute" evidence="1">
        <location>
            <begin position="14"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7751731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="318" mass="35183" checksum="E706CA1B8D7334CA" modified="2024-05-29" version="1" fragment="single">QWRSFEAEKTSIFDRIIQTIGQAIETQDNNEVLAYWLSNASTLLLLLQRTLKASGAAGAPPNRRPSATLFGRMTQSFRGTPQGVNISLIAGETTGASNTLQQVEAKYPALLFKQQLTAYVEKIYGMIRDNLKKDISPLLGLCIQAPRISRANLLKGNAVALASAASQDILIAHWQGIVNNIGCFLNMLKSNNVPPFLVRKIFTQIFSFINVQLFNSLLLRRECCSFSNGEYVKAGLSELEQWCYKATEEVISNMRVLMTEDSNNAVSSSFLLDDDSSIPFSVDDLSKSMDQINIADIEPPPLVRDNSGFSFLLSRAES</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D7M5</accession>
    <name>A0AAD5D7M5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_018798</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="8">Belongs to the DEAD box helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="JAMZMK010002455">
        <property type="protein sequence ID" value="KAI7754799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D7M5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd17966">
        <property type="entry name" value="DEADc_DDX5_DDX17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000079">
        <property type="entry name" value="probable ATP-dependent RNA helicase DDX17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 8" id="KW-0347">Helicase</keyword>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="12">
        <location>
            <begin position="180"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="211"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="11">
        <location>
            <begin position="414"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="72"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="7">
        <location>
            <begin position="180"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="74"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="96"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7754799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="495" mass="53320" checksum="27892D9679EE4B3E" modified="2024-05-29" version="1">MNHYDDRVVDAGSYHQRRSVFMGLPPPAGLPAVGAAPNYGRGGPVPFQGPPPGVHHSAGIGRGIGNGFPPFEPGVGGGGGGRMSNGGHDGGRGRGGRGFDSGGRGGSRGGGGGGGRGGGSKGDLDSITLPKQDFGNLVPFEKNFYIECPAVSAMTDHEVAMYRGRREITVEGHDVPKPVRMFHEAGFPAYCLDVISRLGFHEPTPIQSQGWPMALKGRDLVGIAETGSGKTLSYLLPALVHVAAQPPLAQGDGPVVLVLAPTRELAVQIQQEAGKFGSHSRIRSTCIYGGAPKGPQIRDLQGGVEIVIGTPGRLIDMLEARHTNLRRVTYLVLDEADRMLDMGFEPQIRKIISQIRPDRQTLYWSATWPKEVESLARQFLCNPYKVIIGSPVLKANQSINQIVEVVTDVEKYNRLIKLLKEMMDGNRILIFVETKKGCDQVTRQLRMDGWPALSIHGDKSQDERDWVLAEFKSGRSLIMTATDVAARGLGRITVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DB39</accession>
    <name>A0AAD5DB39_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosome production factor 2 homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Ribosome biogenesis protein RPF2 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_031685</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Nucleus</location>
            <location evidence="1 4">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the RPF2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010001074">
        <property type="protein sequence ID" value="KAI7755511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DB39"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007109">
        <property type="entry name" value="Brix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039770">
        <property type="entry name" value="Rpf2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12728">
        <property type="entry name" value="BRIX DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12728:SF0">
        <property type="entry name" value="RIBOSOME PRODUCTION FACTOR 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04427">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00879">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50833">
        <property type="entry name" value="BRIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Brix" evidence="6">
        <location>
            <begin position="74"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="323"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="338"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7755511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="366" mass="41189" checksum="F7EFE29DC4D3AEB8" modified="2024-05-29" version="1" fragment="single">IATLFLLGFWVFHQKTAFLLLIIILLQVTGNRRVTGENPISQTIYIMLRIKTPKSLRAKRALDKRAPKLVELGKKTLILHGTKTSNTMNAVLTEIYHLKKDNAVRFSRRNDNIRPFESGGETSLEFYSLKTDCSLFVFGSHSKKRPDNLVIGRMYDHHIYDLVEVGVENFKSMSSCSYDKKIAPLIGSKPFFAFIGEGFENVDELKHLKEVLLDLFRGQVVSNLNLAGLDRVYVCTAVSSKKVLFTLCALRLKKSGSVVPRVELVEVGPSMDLVVRRHRLPDEGLKKEAMKVAPELIQKKEKNVSKDAIAGKIGKIYVPDQQVGNASLPFTPKGVKRERREAKGKGEDNKHAEKKQKQKQNDDASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DCS9</accession>
    <name>A0AAD5DCS9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">peroxidase</fullName>
            <ecNumber evidence="3">1.11.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">M8C21_026364</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">AA19_3_7</strain>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 17">
            <text>2 a phenolic donor + H2O2 = 2 a phenolic radical donor + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:56136"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:139520"/>
            <dbReference type="ChEBI" id="CHEBI:139521"/>
            <dbReference type="EC" id="1.11.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="12">Binds 2 calcium ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
        <text evidence="12">Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peroxidase family. Ascorbate peroxidase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.7" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010000431">
        <property type="protein sequence ID" value="KAI7756351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DCS9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140825">
        <property type="term" value="F:lactoperoxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.420.10:FF:000001">
        <property type="entry name" value="Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.420.10">
        <property type="entry name" value="Peroxidase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002016">
        <property type="entry name" value="Haem_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000823">
        <property type="entry name" value="Peroxidase_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019793">
        <property type="entry name" value="Peroxidases_heam-ligand_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31388:SF6">
        <property type="entry name" value="PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31388">
        <property type="entry name" value="PEROXIDASE 72-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00141">
        <property type="entry name" value="peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00458">
        <property type="entry name" value="PEROXIDASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00461">
        <property type="entry name" value="PLPEROXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00435">
        <property type="entry name" value="PEROXIDASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50873">
        <property type="entry name" value="PEROXIDASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0106">Calcium</keyword>
    <keyword evidence="10 13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0349">Heme</keyword>
    <keyword evidence="9 12" id="KW-0408">Iron</keyword>
    <keyword evidence="6 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Plant heme peroxidase family profile" evidence="14">
        <location>
            <begin position="6"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="12">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="85"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="15">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAI7756351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="216" mass="23912" checksum="94EC9A0A8E6F5B69" modified="2024-05-29" version="1" fragment="single">TLKIYVKLKTDQKLTINCLQSGGRRYDVPLGRRDGLVANQSGANIMLPAPFETVGNITRKFEAVGLNITDVVILSGAHTFGKASCATFRNRLFNFSGTNAPDSMLLDPNMVSVLQNLCPVNGNGRQTTALDWNSTENFDNHYFRNLVNGRGLLESDQALYSSDKAFSTTRSLVELYSKNWQIFLDDFKHSMVKLGNIQPLTGSAGEIRKNCRVVNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DES1</accession>
    <name>A0AAD5DES1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">PAP-specific phosphatase, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_007411</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the inositol monophosphatase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000033">
        <property type="protein sequence ID" value="KAI7758047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DES1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008441">
        <property type="term" value="F:3'(2'),5'-bisphosphate nucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000103">
        <property type="term" value="P:sulfate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01517">
        <property type="entry name" value="PAP_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.540.10:FF:000022">
        <property type="entry name" value="Putative PAP-specific phosphatase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.540.10">
        <property type="entry name" value="Fructose-1,6-Bisphosphatase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020583">
        <property type="entry name" value="Inositol_monoP_metal-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051090">
        <property type="entry name" value="Inositol_monoP_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000760">
        <property type="entry name" value="Inositol_monophosphatase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43200:SF4">
        <property type="entry name" value="PAP-SPECIFIC PHOSPHATASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43200">
        <property type="entry name" value="PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00459">
        <property type="entry name" value="Inositol_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00377">
        <property type="entry name" value="IMPHPHTASES"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56655">
        <property type="entry name" value="Carbohydrate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00629">
        <property type="entry name" value="IMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="6">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="333"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR600760-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7758047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="397" mass="43206" checksum="D4382042F8762084" modified="2024-05-29" version="1">MNLLQNPTTKLLQSHRRFTVLSPSLSNRRFSIRSTLPSTAQNAKYNGELRAAVDAVDRACRLCVDVKRSLFTSDGRILEKNDQTPVTIADFGVQALISLELGKLFPSIPLVAEEDSAFLRSNNLVDSVVNVVTDRASCQDKALTQADVVEAIDRRGEYGPKPATYWVLDPIDGTRGFVKGSDALYVVGLALVVEGQIVLGVMGCPNWRVDNSNANEIKNEPGIIMVAHVGCGTWAKGLPDIQNPSVNWYRCLVDGYTLVHKGRYCISESQTWDSLPLSSSFGSTTDVDNVGDEEVLLLPTCCGSLCKYLMVASGRASVFIQRTRTDRVIKVWDHAVGVICVHEAGGKVTDWNGDQLDLAADEVERKALFPPGGILVSNSGLHQEILEIIASKSSVIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G1B4</accession>
    <name>A0AAD5G1B4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_004582</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012171">
        <property type="protein sequence ID" value="KAI7724705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G1B4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010765">
        <property type="entry name" value="DUF1350"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34127">
        <property type="entry name" value="OS04G0405600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34127:SF1">
        <property type="entry name" value="OS04G0405600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07082">
        <property type="entry name" value="DUF1350"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7724705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="438" mass="48442" checksum="E59C0E9293CBD134" modified="2024-05-29" version="1">MTTCWRIPHLQLGSTQFESKLNRSHVRLCDVHHPRSCVRFRKFNRSKVICRNSDDQQSNNQPGGNGIQLYRDIEKLLTETVRQSQDAWGSLGDWQEVDGAWVLRPKNSKPVAVVHFVGGIFVGAAPQLTYRLFLERLSEKGVLVIATPYASGFDHFLIADEVQLKYDRCTRSLYETVQDLPSFGIGHSLGSVIHLLIGSRYAVQRCGNVYIAFNNKDAGLAVPLFSPVLLPVAQSIGPILSQIASSPTIRFGAEMTLKNLENLSPPIMKQVIPLIEQLPPLYMDLVNGRENFSPKPEETQRLIKSYYGISRNLLVKFKDDTIDETPVLAQVLSSESAVSSVLDMSIRYLPGDHGLPLQQALPDVPPGMVDAVNRGGEFWANLTAGTPLETVAKEVSNSLGVDSTAFGADFSKEFDLLVDTIADWIINNAVSGTRLLKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G1K2</accession>
    <name>A0AAD5G1K2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ras-related protein Rab11A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_009704</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Endomembrane system</location>
            <topology evidence="7">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011937">
        <property type="protein sequence ID" value="KAI7725439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G1K2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01868">
        <property type="entry name" value="Rab11_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000274">
        <property type="entry name" value="ras-related protein RABA5a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0636">Prenylation</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7725439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="221" mass="24624" checksum="781D38BFA093CB31" modified="2024-05-29" version="1">MATGYSDPSQKIDYVFKVVLIGDSAVGKSQILARFARNEFSLDSKATIGVEFQTRTLVIQHKSVKAQIWDTAGQERYRAVTSAYYRGAVGAMLVYDITKRQTFDHIPRWLEELRNHADKNIVIILIGNKCDLEDQRAVSTEDAKEFAQKEGLFFLETSALESTNVEDAFLTVLTEIFRIVNKKSLTADEEQNGNPGSLAGKKIIVPGPAQVIPEKKMCCSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G1W9</accession>
    <name>A0AAD5G1W9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_031771</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NDRG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011852">
        <property type="protein sequence ID" value="KAI7725814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G1W9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004142">
        <property type="entry name" value="NDRG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11034">
        <property type="entry name" value="N-MYC DOWNSTREAM REGULATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03096">
        <property type="entry name" value="Ndr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="117"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7725814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="334" mass="36700" checksum="57301CC6EFA3B207" modified="2024-05-29" version="1">MPTMTDSTDSFSIDIDTSSPGGKEHLIETAKGLISVAIFGDQDKPALITYPDVALNYMSCFQGLFLCPEAFSLLLHNFCIYHISPPGHEWGAAPVSRDDPALSADDLADQVADVLDYFGLGAVMCLGVTGGAYVLTLFAIKYTRRVIGLILVSPVCKTPSWSEWLSNKVVSNVLYYYGMCGLVKELLLMRYFSKEVRGGTLVPEADVVQSCRRALGDMQSLNILRYLEALNGRMDITDGLKRLQCRTLIFVGENSTFHLESLHMTSKLDRRFSALIEVGRCGSLVTEEQPDAIPCELNISPRSPLSPTSISPKLFKPESMGLKLKPIKTRVSTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G3I1</accession>
    <name>A0AAD5G3I1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cyclin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_003506</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclin family. Cyclin D subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011396">
        <property type="protein sequence ID" value="KAI7727360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G3I1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd20543">
        <property type="entry name" value="CYCLIN_AtCycD-like_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20544">
        <property type="entry name" value="CYCLIN_AtCycD-like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000069">
        <property type="entry name" value="Cyclin-D5-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039361">
        <property type="entry name" value="Cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10177">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="3 5" id="KW-0195">Cyclin</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="6">
        <location>
            <begin position="74"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7727360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="240" mass="27392" checksum="8ABD3B376D6C78E8" modified="2024-05-29" version="1" fragment="single">MDPHTTFSLPNLMCEEDDSSSNQVKLKIIQPRSEDDHYIQSLIQRETKSIDYGCLCNDDNSNNWFKCARLDTVNWIFSIREILGFNLSTAYLSLTYFDRFNSRRIIDSGKEWAIQLLGIACLSLAAKMEEQTVPLLSQFKAQGYTFESSVIQRMELLVLTTLEWKMCGITPFAYLHYFVSKICDECDCNEVLVSKAIGFVVDFSKEVSLMDHRPSVVAMAAVLLACDDQLTRSTLECKIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G675</accession>
    <name>A0AAD5G675_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_012930</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010892">
        <property type="protein sequence ID" value="KAI7729897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G675"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35830">
        <property type="entry name" value="OS05G0299200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35830:SF1">
        <property type="entry name" value="OS05G0299200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="175"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7729897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="470" mass="53730" checksum="9439940E55830BB6" modified="2024-05-29" version="1">MVKNHYKNQQSRHHALRITTTTHQPPVNKTSSVNAMNSSLNPLHHHYLPKPFHHRYLPKPFHHRYLPKPSPHHHLTTTTTFSSLRRRRHFRRRHPNNNTHIPTNDTVSSPPDSKLHLIIDLHQYIPNKGYKTLLKTAQLQFHNFIQSGNDAVHDLRTLITVDTDRRVMVSCKKSTVYFLGQLIALSLVTVVTFKELRREGGGSVSPLASVEDSILGYSGSMVKNWEKVRKKEVPDWWPEARAAPLEGIDKEENQRKANWLIRAIMDYRTSGKDIQEDDIILLRRICRMSGVNVVIDTSNSRDSIYRASVDFTLNTCGRVPSHSTFVQIDGEDAREFIAGLADNIGLERPRAARIVAAAVAARTRSWLLQAWALEMQGKHIEAVDELSKLCLIHRIFPPDEGSPEMEMVARGLEKHLKLEQRVYLLKMLIGVSTEENRRSLVEALGLEMPAGSVDDEQESMLSLHEDDQER</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G690</accession>
    <name>A0AAD5G690_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">(S)-N-methylcoclaurine 3'-hydroxylase isozyme 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_017605</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011113">
        <property type="protein sequence ID" value="KAI7728938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G690"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000126">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47950:SF49">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47950">
        <property type="entry name" value="CYTOCHROME P450, FAMILY 76, SUBFAMILY C, POLYPEPTIDE 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8 9" id="KW-0349">Heme</keyword>
    <keyword evidence="6 8 9" id="KW-0408">Iron</keyword>
    <keyword evidence="4 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="8">
        <location>
            <position position="427"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7728938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="485" mass="55000" checksum="AB83AA344D67A389" modified="2024-05-29" version="1">MLFSLLFLLPPLFLLFIINIYFKSKKNLPPGPRPWPIIGNLHQVGENPHVSTAILAQKHGPLISLRLGTKVVIVASSPEAAMGILKTQDRFLSSRFVPNAFHCKYLPFALVWSVDCNENWKSLRTLCRTEMFSVKALECQSSLREEKLAQMIDFLCSKDGQVVNIEEVVFITMFNTLSNIFFGKDFLDLKDQRGTASGLKEKLIKILENGLAPNISDFFPILQRLDLQGLRKQNLKHMEEVFDSWVGTINERRLAVASQKEQCFLDGLIKNGFTNEQINILALELFTAGTDTTTSTIEWAMAELIKNKNVMFELQQELKHKIKSSTILESEISKLPYLNACIKETLRLHPPVPLLLPHRATQTSKVLNYTVPRGAQILVNIWAIGRDPKLWEDPFSFKPERFLGSSLDFRAGQDFEFIPFGAGRRMCPGLPSGITSVQSILASLILRFDWVLPNDQDPVKLNMNEKFGVTLAKEKPLQLIFKRLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G6Z3</accession>
    <name>A0AAD5G6Z3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_004455</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010945">
        <property type="protein sequence ID" value="KAI7729673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G6Z3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057852">
        <property type="entry name" value="Beta-prop_WDR11_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057853">
        <property type="entry name" value="Beta-prop_WDR11_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057854">
        <property type="entry name" value="TPR_WDR11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039694">
        <property type="entry name" value="WDR11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14593">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14593:SF5">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23751">
        <property type="entry name" value="Beta-prop_WDR11_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23752">
        <property type="entry name" value="Beta-prop_WDR11_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23753">
        <property type="entry name" value="TPR_WDR11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WDR11 first beta-propeller" evidence="2">
        <location>
            <begin position="1"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="WDR11 second beta-propeller" evidence="3">
        <location>
            <begin position="169"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="domain" description="WDR11 TPR" evidence="4">
        <location>
            <begin position="591"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="340"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="340"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7729673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="1001" mass="109495" checksum="20A258E0149ECE8B" modified="2024-05-29" version="1" fragment="single">GEQVHVMCMIEELMPALGTPVPSPSVLAVVISRSDSTLQNVRKSLCDGHQNTEVDMDFDNPFDFCDESQVVSKTHLLSISDDGKIWKWLLTAEGSTENQKDTSDGIVAEISKDPALDTNADGLDSSSGFVKDVIKQTDKENIRKGHSSASKRSQDDLALKISLVGQLHLLSSSVTMLAVPSPSLTSTLARGGNHPAVAVPLVALGTQGGSVEVIDISANAVAASFSIHDSVVRGLRWLGNSRLVSFSYSQGNEKSGGGYTNRLVVTNLRTGLNRTFRVLQKPERAPIRALRASSSGRYLLILFRDAPVEVWAMTKTPIMLRSLALPFTVLEWTLPTVPRPTQSGVSKQSDASVPPTGMASQAESRALGPDGAQEEFSESFAFALVNGALGVFEVQGRRIRDFKPKWPSTSHISADGLVRAMAYRLPHVVMGDRAGNIRWWDVTTGQSSSFNTHREGIRRIKFSPVVPGDRSRGRIAVLFNDNTFAVFDLDSPDPLANSLLQPQFPGTLVLELDWLPVRTDKNDPLVLCITGADSSFRLVEVNVDKRVGYEAQTGAIKERFRPMPLCSPILLPTAHALALRLILQCGVKPSWFKTNNILINKENSQLSKSSSVDLRSYLIDIPTIGDSVVPELLLKVLEPYRREGCILDNEIVRQYAAILNKGSAARFSFAAAIFGENSEAYFWLQLPRALKHLMNNLANKSLQKNPVISSSSSETDDVSLLTRISSKGKSGTGSSKSNTVSDGELRLMAFEQDELWERANERITWHEKLGDEEAIQNRVHELVSIGNLEAAVSLMLSTPPESPYFYPNALRAVALSSAVSKSLNELAVKVVAANMVESDRSLAGTHLLCAVGRYQEACSQLQDAGCWTDAATLAATHLKGTDYARVLHRWAEHVLHAEHNIWRALILYVAAGSLQDALSALRDAQQPDTAAMFIIAFREIHAEFIQNLNSDDDSDSLIKEKLLVLPGLNPESEDVIAIGELYGQFQQKLVHLCMDSQPFSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G909</accession>
    <name>A0AAD5G909_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Receptor-like serine/threonine-protein kinase</fullName>
            <ecNumber evidence="17">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">M8C21_004136</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="24">AA19_3_7</strain>
            <tissue evidence="24">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16 17">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15 17">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="17"/>
    <dbReference type="EMBL" id="JAMZMK010010365">
        <property type="protein sequence ID" value="KAI7731846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G909"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048544">
        <property type="term" value="P:recognition of pollen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00028">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01098">
        <property type="entry name" value="PAN_AP_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000537">
        <property type="entry name" value="Putative receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000059">
        <property type="entry name" value="S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.90.10.10">
        <property type="entry name" value="Bulb-type lectin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.4.10">
        <property type="entry name" value="Hepatocyte Growth Factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001480">
        <property type="entry name" value="Bulb-type_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036426">
        <property type="entry name" value="Bulb-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000858">
        <property type="entry name" value="S_locus_glycoprot_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024171">
        <property type="entry name" value="SRK-like_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47974">
        <property type="entry name" value="OS07G0415500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47974:SF3">
        <property type="entry name" value="RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01453">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00954">
        <property type="entry name" value="S_locus_glycop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000641">
        <property type="entry name" value="SRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00108">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51110">
        <property type="entry name" value="alpha-D-mannose-specific plant lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50927">
        <property type="entry name" value="BULB_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50948">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 17 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 17 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0675">Receptor</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 17" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6 20" id="KW-0732">Signal</keyword>
    <keyword evidence="4 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041965441" description="Receptor-like serine/threonine-protein kinase" evidence="20">
        <location>
            <begin position="18"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="397"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="463"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="domain" description="Bulb-type lectin" evidence="22">
        <location>
            <begin position="20"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="23">
        <location>
            <begin position="338"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="21">
        <location>
            <begin position="516"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="544"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRNR" id="PIRNR000641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="KAI7731846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="808" mass="90716" checksum="71597C27AB7879A7" modified="2024-05-29" version="1">MNLLFSLILCLASLTSSSSSSSSSPPSALKLDSSLKVDNKHDFLISPNNVFTAGFYSVGDNAFCFSIWFTKPLSNGERTVVWMANRDAPVNGKRSKLSLTTTGNLILLDADQELPIWTTRTSDPTQSAELKLNNSGNLFLQSKDEYIIWQSFGSPTDTLLPNQPFTKDVTLVSSRSRTNYSSGFYKLIFDNDNVIRLVYNGPNVTGVYWPNPELTASDSGRNTYGSERIATIDQFGRFVSSDGLVFSTLDYGGQPMRRLTLDVDGNVRVYSFNDGTKVWDVTWQAIAQTCTIHGTCGENSTCTNVPNFGKKCTCLPYHKMVNETDWSYGCEPEFKDSCGNVNEYGFVHLAHFDFYGYDLRYMPNASLGVCMQECKKVCNCKGFQYKFDKEQGIYLCYLKFLLLNGYGSVSFNGSIYLRVPMSILSSNSMKRIQELGLNCSGIAKMVQLERVYVKKKEQRSLKFLLLFTYVFGAFEVLCIVYIFYKTRNNVQSSEGYLQVATGFERFSYADLRKASENFKIEIGRGGGAVVYKGILSDNRVAAIKCLKESNSNQGEVEFLAEVSTLGRLNHMNLIDIWGYCAEGNHKILVYEYMENGSLAENLHANDKLDWDKRFEVAVGTAKGLAYLHEECLEWVLHCDVKPHNILLDHDYSPKVADFGLLKFMDRDGRGNSEFTKARGTRGYMAPEWFYVNLPITSKVDVYSFGVVMLEMITGKGPQGGGDQNDGYKKPVVSWVRDKVAVAAEADGCDDMSDWIEEIVDSTVKGEYDRIRMGTLIKVALQCVEENKDQRPTMSQVVDMLLHGDDCKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G9S8</accession>
    <name>A0AAD5G9S8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_019881</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010245">
        <property type="protein sequence ID" value="KAI7732403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G9S8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13999">
        <property type="entry name" value="STKc_MAP3K-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000193">
        <property type="entry name" value="Serine/threonine-protein kinase CTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF255">
        <property type="entry name" value="OS02G0527600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000654">
        <property type="entry name" value="Integrin-linked_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7732403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="252" mass="28652" checksum="14F50034617DF6A9" modified="2024-05-29" version="1">MRRLRHPNIVLFMGAVTQPPNLSIVTEYLSRGSLYRLLHKPGAKETLDERRRLSMAYDVAKGMNYLHRRNPPIVHRDLKSPNLLVDRKYTVKVCDFGLSRLKANTFLSSKTAAGTPEWMAPEVLRDEPSNEKSDVYSFGVILWELATLQQPWGNLNPAQVVAAVGFKCKRLEIPPDVNPQIAALIEACWANEPWKRPSFSAIMETLRPLTKPPTPHTRIVGITMLSFVKPYPTFADRNHMLHNIRFGVEVVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GA21</accession>
    <name>A0AAD5GA21_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ABC1 atypical kinase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_031926</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. ADCK protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009888">
        <property type="protein sequence ID" value="KAI7733899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GA21"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05121">
        <property type="entry name" value="ABC1_ADCK3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004147">
        <property type="entry name" value="ABC1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050154">
        <property type="entry name" value="UbiB_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10566">
        <property type="entry name" value="CHAPERONE-ACTIVITY OF BC1 COMPLEX CABC1 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10566:SF119">
        <property type="entry name" value="OS04G0640500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03109">
        <property type="entry name" value="ABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC1 atypical kinase-like" evidence="2">
        <location>
            <begin position="161"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7733899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="723" mass="81085" checksum="800595A07D0F3CED" modified="2024-05-29" version="1">MILSPPPPSLYPTTRHHHHTRRLTVNFLRTPTLRHRTTTVQSAARDVDTFTESSGYLFELSTSEAESLTEYNISKISSIYLKKPLIVLRRLLQIGNTLGKWLAVRYFDSVSDRSDLMFEVRASQLREILAKLGPAYIKIAQAVSSRPDLIPPSYLNELSLLQDRISPFSTEIAFNTIEKELGLPLNQLFSEITPEPVAAASLGQVYQARLQDTGQIVAVKVQRPGVQAAISLDILILRVLAGLIRKAGKFNTDLQAVVDEWASSLFREMDYVNEAKNGVRFRELYGDLKDVLVPDFYVSKTTRKVITMQWIEGQKLSEVKDLYLVERTTARGRLGFITMPFNASSCLNNYIGKRQVGVYCSFNQLLEYGFYHADPHPGNLLHFGMMGEFKQELRDGFIEACIHLINRDYDSLAKDFVTLGLLPATADKDAVTKALTGVFQNAVSKGVRNISFGDLLGNLGTTMYKFKFQIPSYFSLVIRSLAVLEGIAINNDPNYKVLGGTYPWIARKVLTDSSPQLRSSLQSLLYEDGVFRIDRLESLLSESLRARTERALVKIEDDGSNEAIKQIMSFTLTEKGAFVREILLQEFAKGLDALGLATLESWTSAATANLPFAPSPSFLLTDEDMINLRNLHRLLQLLSGSPNIEDADSGVEAFNQYTKKRTNSEEVSLIFNEFTSVQDIVPLLNVIPELPPDLQQQLIRLPVDLVGKFISRVAARTLRRALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GA59</accession>
    <name>A0AAD5GA59_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Auxin response factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_017905</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Auxin response factors (ARFs) are transcriptional factors that bind specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs).</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimers and heterodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the ARF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009853">
        <property type="protein sequence ID" value="KAI7734004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GA59"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009734">
        <property type="term" value="P:auxin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10017">
        <property type="entry name" value="B3_DNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.1040:FF:000001">
        <property type="entry name" value="Auxin response factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.330.10:FF:000001">
        <property type="entry name" value="Auxin response factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000047">
        <property type="entry name" value="Auxin response factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.330.10">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010525">
        <property type="entry name" value="ARF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044835">
        <property type="entry name" value="ARF_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003340">
        <property type="entry name" value="B3_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015300">
        <property type="entry name" value="DNA-bd_pseudobarrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053793">
        <property type="entry name" value="PB1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31384">
        <property type="entry name" value="AUXIN RESPONSE FACTOR 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31384:SF150">
        <property type="entry name" value="AUXIN RESPONSE FACTOR 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06507">
        <property type="entry name" value="ARF_AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02362">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01019">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101936">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50863">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51745">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0927">Auxin signaling pathway</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TF-B3" evidence="11">
        <location>
            <begin position="127"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="PB1" evidence="12">
        <location>
            <begin position="716"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="688"/>
            <end position="715"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7734004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="850" mass="93992" checksum="6124939E657AAED5" modified="2024-05-29" version="1">MKLSSVGFDQQLPEGEKRCLNSELWHACAGPLVSLPTAGSRVVYFPQGHSEQVAASTNKEVDAHTPNYPSLPPQLICQLHNVTMHADAETDEVYAQMTLQPLNAEEQKEAFLPADLGAPSKQPTNYFCKTLTASDTSTHGGFSVPRRAAEKVFPPLDYSQQPPAQELIARDLHDNEWKFRHIFRGQPKRHLLTTGWSVFVSAKKLVAGDSVLFIWNEKNQLLLGIRHANRPQTVMPSSVLSSESMHLGLLAAAAHAASTNSRFTVFYNPRACPSEFVIPLAKYVKAVYHTRVSVGMRFRMLFETEESSVRRYMGTITGIGDLDQTRWPNSHWRSVKVGWDESTAGERQPRVSLWEIEPLTTFPMYPSQFPLRLKRPWPLGLPSFNGIKEEDLGMNSPLMWLRGNPCDRGIGSFNFQGLNGPQWLQPRLDMGMVGMQTDIYQAMAAAALQEMRSIDSLKPSNPSLLQFQQHQTVPAGSVPAQMVHQSQSQPTFVPENLPVSHQQPHLLSQQLNHQNPFNSQQQTLLPMIPSVQNQNFSSSSGNPVSLLGSFSHNETSHLLNMPRSTSMLTSAGWPAKRVAVDPLLVSGSSQSLLHQVEQLGPPSHTNLSQNTSLSQNAVSLPPFPGRECSIDQEPNNDPQSNILFGVSIDNPNLFLQGSDGDSLTMPLTSSNYNMSNAGNGFSTNPTVTPSSCVDESGFLQSPDNAGQTGQANPPTRTFVKVHKSGCFGRSLDIGKFSSYHELRRELGQMFGLEGQLEDPLRSGWQLVFVDRENDVLLLGDDPWPEFVNSVWCIKILSPQEVQEMGKQGLELLNSVQLPKPSDNSYENYAPSRQESMSLSNGVASVGSLEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GAS9</accession>
    <name>A0AAD5GAS9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_004941</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Endomembrane system</location>
            <topology evidence="7">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009569">
        <property type="protein sequence ID" value="KAI7735072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GAS9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000274">
        <property type="entry name" value="ras-related protein RABA5a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0636">Prenylation</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7735072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="164" mass="18302" checksum="7DF48770913805EB" modified="2024-05-29" version="1">MQVEGKIVKAQIWDTAGQERYRAITSAYYRGAVGALLIYDITKAQSFENVKRWLRELRDHADSNIVIMLAGNKADLSHHRAVQETDGQKLAEQEGLVFLETSAFEAYNVEKAFQMILLDIYKIVSKKALAAQEAASASVPVPSQTTTIKVGDYDKNSRRKCCSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GB49</accession>
    <name>A0AAD5GB49_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_001399</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009870">
        <property type="protein sequence ID" value="KAI7733976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GB49"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990246">
        <property type="term" value="C:uniplex complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036444">
        <property type="term" value="P:calcium import into the mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051560">
        <property type="term" value="P:mitochondrial calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039800">
        <property type="entry name" value="MICU1/2/3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12294:SF29">
        <property type="entry name" value="CALCIUM UPTAKE PROTEIN 1_2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12294">
        <property type="entry name" value="EF HAND DOMAIN FAMILY A1,A2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="192"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7733976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="237" mass="26491" checksum="8C05D32800FC33A2" modified="2024-05-29" version="1">MSSLRRTAPSIHRHLRSLSTASSSFITTPITTTTTSNSNNQNPNLIRWISAVVAGTGIGAGLSLYWCYPSLSSTYAVKSVMSFSDWLTATVDDIKKSYHGQTLSSSNFFIRDALRRRIFFNYEKRLRSLSPPEKVFEYFASVQNSEGETLMTPGDLMRAIVPVFPSSESNNVREGCLRGERLPGELHCAPSQFFMLFDVNNDGLISYKEYIFFVTVLAIPESSLSVAFKMFDIDNDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-10-08" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GDE6</accession>
    <name>A0AAD5GDE6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ketoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_023197</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009369">
        <property type="protein sequence ID" value="KAI7736108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057326">
        <property type="entry name" value="KR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045000">
        <property type="entry name" value="TR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42898">
        <property type="entry name" value="TROPINONE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42898:SF28">
        <property type="entry name" value="TROPINONE REDUCTASE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SMART" id="SM00822">
        <property type="entry name" value="PKS_KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ketoreductase" evidence="3">
        <location>
            <begin position="14"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7736108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="284" mass="30563" checksum="CA7D391A20B9B197" modified="2024-05-29" version="1">MSTSREQRWSLKGMTALVTGGTRGIGYAIVEELAGFGAAVHTCSRNQKEIDERLEEWKGKGYEVTASVCDLSSKENREELIKVVSSAFNGKLNILINNAASTLLKDATQHTLDDYASIMRTNVESPYHLTQLAHPLLKASGNASVVFISSVAGVIALPSISVYAASKVDQELXXXXQHTLDDYASIMRTNVESPYHLTQLAHPLLKASGNASVVFISSVAGEKIEDKVMEAYGELMARTPLRPIAEPDEISPLVAFLCLPGASYISGQVIVVDAGYTAGGFKQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GDU5</accession>
    <name>A0AAD5GDU5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_000482</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009257">
        <property type="protein sequence ID" value="KAI7736498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GDU5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13999">
        <property type="entry name" value="STKc_MAP3K-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000439">
        <property type="entry name" value="Integrin-linked protein kinase family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000763">
        <property type="entry name" value="Os01g0748600 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000180">
        <property type="entry name" value="serine/threonine-protein kinase STY46-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF140">
        <property type="entry name" value="INACTIVE PROTEIN TYROSINE KINASE PTKL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000654">
        <property type="entry name" value="Integrin-linked_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="88"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="138"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="19"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7736498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="474" mass="53797" checksum="C9E082AE211CAD15" modified="2024-05-29" version="1">MDAKGKVRFTIGKQSSMAPDREPDRDRDRETDRDKDREQDRDRDSDSDDGMEEIDPRVRLMFYASEGDLEGIKKLMKAGTDVNFKDIDNRTALHVAACQGFSDVAKLLLEGGADVDPKDKWGSTPLADAIHYKKHGVAKLLEKHGAKHLMAPMHVNNAREIPEYEIDAKELDFTNSIDITKGTFTMASWRGTQVAVKKLGDELCTDEDKVAAFRDELELLQKIRHPNVVQFLGAVTQSSPMMIVIEYLPKGDLKAYLKRKGPLKPLTALKFAMDIARGMSYLHEIKPEPIIHRDLEPSNILLDDSGHLKVADFGVSELLKGTNAAKHKKLMNYHDTSSRRYVAPEVLKNEDYDTKVDVFSFALILQEMIEGCQPFATKDDKDVPKLYAAKRRPPFNAPSKSYPNGLKQLIEECWHENPSKRPSFRQIIVRLESIYNSINHKNHWKFQPLKCFGLTSMCKGDDSDTSSYNRSSRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GG28</accession>
    <name>A0AAD5GG28_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_026494</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008242">
        <property type="protein sequence ID" value="KAI7741205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GG28"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31267">
        <property type="entry name" value="DENTIN SIALOPHOSPHOPROTEIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31267:SF2">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="203"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="306"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="138"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="221"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="306"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="655"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7741205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="655" mass="71618" checksum="78F439CE090C356E" modified="2024-05-29" version="1" fragment="single">IPEPENSDGSFGGHQRSQSSNSQGIGLQLGPPSQRLPMAMQAVKHNSFSQSQPSANSRGKGHAESSPFPPFQETSHGEFKSDRMSISGQSESQTMGNKMTNLSAALGSAEFPNSRNHLQNHPKAGNESFDGPASQIRQADERVQVSHSLPGEKLHASHPHASSGIQGAFPKMLSNASSNLPSQQLFSATQPHKSQFNIVESTSIGQQNSEEQDSEKRENKSSLNAQGINTSVGELSTEKVDLGQMMNGPAASQKGLEAFGRSLKPNNFHQQISLMNQMKAYKHTDNDPNNRVLNILKDSDNLLHGQQVTVSPGNSELPRSTLPTDNVGRSLASKDAPASDQNVPTFVKIDKSHISPQMAPSWFDQHGTLKTGQMSSQIADAVRTVEQPFKVSGRLETTAHISKETSASGASDNSKDGVIWRTPAPSSVVVDHRFTSQPLQPSITVQNLVPLRPKKRKWSRDEIRSWLKEDGFDGMVEDDGQLIEDIPRPKRRIILATQLMQQLFPPPPPVFLSTKATSNYESVAFYASRLALGDACNLVSCVSHKKSNLITEKSTESEKVDDNRLSKAVEDCLTKAKWVEGTFSRLDKRASILDLRVECQDLEKFSIINRFAKFHGRGQTDGESSSLSDAPANAPKPFPQRYVVAVALPKNLPER</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GGW9</accession>
    <name>A0AAD5GGW9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_003951</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008627">
        <property type="protein sequence ID" value="KAI7739453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GGW9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="206"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="250"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="323"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="413"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="527"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="704"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="781"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="259"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="323"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="361"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="418"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="434"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="545"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="573"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="606"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="644"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7739453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="827" mass="92402" checksum="4230F05CF12D7342" modified="2024-05-29" version="1" fragment="single">MAHDDGVANHHMVFIDTSLDTHLAIIVSDSDTVSDFKRNIMLEHRQLFPAIGDVKIECLKVKRRGSFYKLSESMLVKSAFEYAKGNWFVSVDASRLEQNDGSQQLGKHKDRDQLALPWVTHSRSIERHGNDGSPSVHSKTAPLVNQKVPIVDQLTSGDSCKDVSKNVKEDRLCNDKQLDYELQEKLDTANTDINLKKRTRDVHNEGSLNDAFLSGPSLKKKRKTQRGKSDVKALEENLALRHDSDKVNDKVANVNNDSSFEHRSKGKKLSDENQRINKGENCSALDQVATVVPTIEGVGSETVGDVAMVMENNIQQEIAIGRSQKDTDSEVVQKPDMGDEPSSLMRTPDCEHGGAGVTDVGHMKRSTEHKAAEKEASSTASIKKLHSTPKNAIESSPDVVANEGILLSRSECPVNNDADISTTGKNTSQVNAQEKQDEKMDDTTKKKKRRRTKVSAGRNEDELLTKHVNRDICESVKEGESVWPDTDRTDVDMSSTVLASVIDERKMNEDLEKTLIDGKKGDDVIMKEVENSVENMEEESRKEFTNANDVVTGTNEENLLPQKQNDKTDDTTKKKKKRKTKKSAGRNEDESVPKNVDGDIFENVIEGDRKDGDKVKEKDARISYDGLAGKESKNNKSQDLQVELESKKSQSVDHHHLPEKTSENITASDVSKVNNEVDIPETESRDLAFRDCFANGHLSKKIVSTDNRKDKKKTKVDSRNDQSQKLVSKIENNKFSVDVLKDPNITDIDKFKTPKRANKIDAKKTNRQPTTLLQTRAKTMENKKVKGSQPVKRSHARKGMEGAGKNNMNSQLYRGGRWEGWAALVLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GH75</accession>
    <name>A0AAD5GH75_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Homeobox-leucine zipper protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">HD-ZIP protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Homeodomain transcription factor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_019415</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Transcription factor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 10">Belongs to the HD-ZIP homeobox family. Class I subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007971">
        <property type="protein sequence ID" value="KAI7742495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GH75"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000200">
        <property type="entry name" value="Homeobox-leucine zipper protein ATHB-13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045224">
        <property type="entry name" value="HDZip_class_I_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000047">
        <property type="entry name" value="HTH_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003106">
        <property type="entry name" value="Leu_zip_homeo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24326">
        <property type="entry name" value="HOMEOBOX-LEUCINE ZIPPER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24326:SF615">
        <property type="entry name" value="HOMEOBOX-LEUCINE ZIPPER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02183">
        <property type="entry name" value="HALZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00031">
        <property type="entry name" value="HTHREPRESSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 8 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 8 9" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6 8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 10" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="13">
        <location>
            <begin position="61"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="8">
        <location>
            <begin position="63"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="41"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="113"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="51"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU369038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7742495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="276" mass="31495" checksum="A0ACD5AF878DC65B" modified="2024-05-29" version="1">MSFLPPEPMFFGYEDDHHPPHDFQGVQTLMMRRSMSYSGMDRCEETRGPAADDDMSEDDGTQLGEKKRRLNLEQVKALEKSFELGNKLEPERKTQLARALGLQPRQVAIWFQNRRARWKTKQLEKDYDVLRRQYESVKADNDALKNLNKKLHAELLAAKGGEANGVRPINLNKETEGSWSNGSENSCDLNTVTCNMTQSEESTMFYTQISNMYPTMSSMGPNSGLTQLLQSPLTTDLLGQRLNQTVGNEGLCNMFNGIEDQPAYWPWPEQNPQQLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GHF0</accession>
    <name>A0AAD5GHF0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">COBRA C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_022028</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the COBRA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007917">
        <property type="protein sequence ID" value="KAI7742750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GHF0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010215">
        <property type="term" value="P:cellulose microfibril organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052324">
        <property type="term" value="P:plant-type cell wall cellulose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056900">
        <property type="entry name" value="COB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006918">
        <property type="entry name" value="COBRA_pln"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31673">
        <property type="entry name" value="PROTEIN COBRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31673:SF61">
        <property type="entry name" value="PROTEIN COBRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25079">
        <property type="entry name" value="COB_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04833">
        <property type="entry name" value="COBRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038122">
        <property type="entry name" value="COBRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="407"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="COBRA C-terminal" evidence="8">
        <location>
            <begin position="218"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="COBRA C-terminal" evidence="8">
        <location>
            <begin position="260"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF25079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7742750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="426" mass="48101" checksum="85CD9B37A738BFDC" modified="2024-05-29" version="1" fragment="single">KSFCVFLDGLLIKPSSSLLAEAYDALDPNGNITIKWDIISWTPDGYVAVVTMFNFQQYRHISAPGWTLGWTWAKKEVIWSMMGGQATDQGDCSRYKTSPPHCCKKTPSIVDLLPGTPYNQQIANCCKGGVINSWAQEPNNYASSFQLSVGAAGTSNKTVRPPKNFTLLAPGPGYTCGPAVVGKPSKFISADGRRVTQAMMTWNVTCTYSQFLAQKTPTCCVSLSSFYNETIVPCPTCTCGCQNNDTHPGINGPGKNSLTPLVQCTRHMCPIRVHWHVKLNYKDYWRVKVTITNFNYRMNYSQWNLVVQHPNFDNLTQIFSFNYKPLTPYSSINDTAMLWGVKFYNDFLNQAGPMGNVQSELLFRKDKSTFTFEKGWAFPRRVYFNGDNCVMPPPDAYPHLPNASSRVKWSLFVFFMTFLTFFFLFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GIA9</accession>
    <name>A0AAD5GIA9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GDSL esterase/lipase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_002472</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008033">
        <property type="protein sequence ID" value="KAI7742284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GIA9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01837">
        <property type="entry name" value="SGNH_plant_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035669">
        <property type="entry name" value="SGNH_plant_lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22835:SF476">
        <property type="entry name" value="OS06G0160200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22835">
        <property type="entry name" value="ZINC FINGER FYVE DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042034250" description="GDSL esterase/lipase" evidence="5">
        <location>
            <begin position="17"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7742284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="354" mass="38978" checksum="71B2C3FE9A2F7F7F" modified="2024-05-29" version="1" fragment="single">FRMVMVVALVATLCEAGCEYEAIFNFGDSNSDTGGFWSVFPEAPPPNGMTYFKHPVGRACDGRLIVDFIAERIGIPFVSPYLMSIGSDYRHGANFATLASTVRLPQTSLFVSGLSPFALPIQVNQLKHFKAQVENLYSQGSTNLPAPDIFGKSLYTFYIGQNDFTSDLAKNGINRVKEILPQDLYALGGRAFFVLNLAPIGCYPSFLVELPHGDKDIDEFGCLISYNNAVDEYNDMLRDALNQTRQQLSDAKVIYVDSHRALLELFRHPTSHGLKYGTIACCGNGGGRYNFNPQVFCGNTKFINGQNLTASACDDPENYVSWDGVHTTEAANKIIVDAIFSGDFTDPPFEIQCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GJQ5</accession>
    <name>A0AAD5GJQ5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Agenet-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_011209</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007265">
        <property type="protein sequence ID" value="KAI7745390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GJQ5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008395">
        <property type="entry name" value="Agenet-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36805">
        <property type="entry name" value="AGENET DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36805:SF7">
        <property type="entry name" value="AGENET DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05641">
        <property type="entry name" value="Agenet"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Agenet-like" evidence="2">
        <location>
            <begin position="10"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="253"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="274"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="314"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7745390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="323" mass="36571" checksum="8816FCA2911D71D4" modified="2024-05-29" version="1">MGNPLNFQVGQLAEMKSFEDGFRCAWFRCKIKDIDLKKKMILPEYYDFDPAELTWEEIYQEGPSSRKSKRVKRYLMMRPEYPMTYKKSENMPPLDSISQVCVIVDGTWKVGDLVDWFTDGCYWSATVVKVLSNDKFQIEIFYESAGKLAKQKALCTFENISFMMTIIATFRKEHKVELPRPPLGNGEEGKEGKYDYEACCKDFRPSLIWSATKGWTLPTVEGRTCDAQLIFPRKQGKSTKLVFVIFNLSGMDLEPEPEPVPAAAGSTARTSAEVNKDSEQNEESVKMDCDVGMDLEPQPGPEPATAGSTTRMSAEGDRDGEQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GLI9</accession>
    <name>A0AAD5GLI9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_010516</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007462">
        <property type="protein sequence ID" value="KAI7744701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GLI9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7744701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="72" mass="7927" checksum="779636437A23050F" modified="2024-05-29" version="1">MLAMFSPLLPAPQCADQLVQVRRGCGVHGFCAGFDLVSTVRLCWVVLWSCASRFNLAIGNLRVLFGLALRPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GLK6</accession>
    <name>A0AAD5GLK6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_016881</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LOR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007447">
        <property type="protein sequence ID" value="KAI7744746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GLK6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.200">
        <property type="entry name" value="LURP1-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007612">
        <property type="entry name" value="LOR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038595">
        <property type="entry name" value="LOR_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025659">
        <property type="entry name" value="Tubby-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31087">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31087:SF122">
        <property type="entry name" value="TUBBY-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04525">
        <property type="entry name" value="LOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54518">
        <property type="entry name" value="Tubby C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7744746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="114" mass="12656" checksum="3F2FA3807DC596EA" modified="2024-05-29" version="1">MIQFKTNISMKLANKMSNNDDCDLRIKGSWLKGNCTIYMGDSSKVVAEMHKLQTSKYANDKFTVTIQANMDYACVVALLAIVDVMENPDEKKRYDVPLEDVVDAIGAGLKATSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GM28</accession>
    <name>A0AAD5GM28_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Small VCP/p97-interacting protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_005542</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007092">
        <property type="protein sequence ID" value="KAI7745914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GM28"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031632">
        <property type="entry name" value="SVIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36813">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36813:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15811">
        <property type="entry name" value="SVIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="1" id="KW-0519">Myristate</keyword>
    <keyword evidence="2" id="KW-0564">Palmitate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="16"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="16"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="50"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7745914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="77" mass="8425" checksum="75F3AB12EC2D9DF4" modified="2024-05-29" version="1">MGCFGCFDGGLAKQERLEEERRASQEARAKAAEAAERRQEEFAKSAVGRAARAQMQAAAKQASHTNKGEPTLKWQMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GMT5</accession>
    <name>A0AAD5GMT5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">CCR4-NOT transcription complex subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_012430</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006496">
        <property type="protein sequence ID" value="KAI7748630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GMT5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030015">
        <property type="term" value="C:CCR4-NOT core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000289">
        <property type="term" value="P:nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.180:FF:000012">
        <property type="entry name" value="Ccr4-Not transcription complex subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.840">
        <property type="entry name" value="CCR4-NOT transcription complex subunit 1 TTP binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032191">
        <property type="entry name" value="CNOT1_CAF1_bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024557">
        <property type="entry name" value="CNOT1_dom_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032193">
        <property type="entry name" value="CNOT1_TTP_bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038535">
        <property type="entry name" value="CNOT1_TTP_bind_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040398">
        <property type="entry name" value="Not1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13162">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13162:SF8">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16415">
        <property type="entry name" value="CNOT1_CAF1_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16417">
        <property type="entry name" value="CNOT1_TTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12842">
        <property type="entry name" value="DUF3819"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1 TTP binding" evidence="9">
        <location>
            <begin position="2"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1 CAF1-binding" evidence="8">
        <location>
            <begin position="228"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1" evidence="7">
        <location>
            <begin position="570"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="452"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="760"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="859"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="760"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF12842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF16415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7748630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="922" mass="99700" checksum="D9E4AFFCA099F138" modified="2024-05-29" version="1" fragment="single">MLIKNQLVTHLTLGIALRAVLDALRKPADSKLFIFGTKALENFVDRLIEWPQYCQHILQISHLRGAQPELVAFIERALARNSSGHPESDAGYSPSADQHHNYILQPNTEVVGSSYPLTGTGSSHLVSHISSPIQLQQRNQSYIDERHRASVTSSGYVKPNLNTTGQVSVALANEPVNIPKPLSVASSSAALASSPAFARPSRAVTSAKFGSLNIETLVAAAERRETPIEAPPSETQDKISFIINNLSTANIEAKAKEFTEILKEPYYAWFAEYMVMKRASIEPNFHDLYLKFLDKANSKPLNKEIVQATYENCKVLLGSELIKSSSEERSLLKNLGGWLGKITIGRNHVLLAKEIDPKPLIIEAYEKGLMIAVIPFTSKILESCSKSLAYQPPNPWTMGVLGLLAEIYAMPNLKMNLKFEIEVLFKNLNVDFKEVTPSSLLVDKVREVDSNPDFSNKDIGPTQQPMVGEVKSSKRSSLNQVELPVEIGSSSHSGGHPRILSQYAAPHHFPAVTLSEDEKMAALSLSDKLPSAQGLLQAQLPFSVGQLPSSSMDQQVIINPKLQALGLHMHFQRVLGISMELAIKEIVSSIVQRSVSIATQTTKELVLKVDYAMESDESRIHSAAHLMVASLAGSLAHVTCKEPLRGSISSQLRNNLQALSGNISSELLEHAVQLVTNDNLDLGCAFIEQAATEKAVQTIDSELAPQLSIRRKHREGVGSTYFDASLYTQGYLGVLPEALRPKPGRLSHSQQRDFVRLPWQNQSNQNPNSVSLGPSPSPGSGTLHGGYSSGTGSVSVSGQLNPGIFSSGINAVGHSMASEDMEPASLKLLSGSSVHSTMGGGTGSHNFEGGLSAPAPEVHVPGSSSAPKELGVHAQPLSSPSDHIINTTAEPSLTTKDAVAKYQFISEELESLIAKDAKEAEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-02-05" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GNC6</accession>
    <name>A0AAD5GNC6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">V-type proton ATPase proteolipid subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_029140</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Proton-conducting pore forming subunit of the membrane integral V0 complex of vacuolar ATPase. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex attached to an integral membrane V0 proton pore complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Vacuole membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the V-ATPase proteolipid subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006402">
        <property type="protein sequence ID" value="KAI7749125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033179">
        <property type="term" value="C:proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18175">
        <property type="entry name" value="ATP-synt_Vo_c_ATP6C_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18176">
        <property type="entry name" value="ATP-synt_Vo_c_ATP6C_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.610:FF:000007">
        <property type="entry name" value="V-type proton ATPase proteolipid subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.610">
        <property type="entry name" value="lithium bound rotor ring of v- atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002379">
        <property type="entry name" value="ATPase_proteolipid_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000245">
        <property type="entry name" value="ATPase_proteolipid_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011555">
        <property type="entry name" value="ATPase_proteolipid_su_C_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035921">
        <property type="entry name" value="F/V-ATP_Csub_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01100">
        <property type="entry name" value="V_ATP_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10263">
        <property type="entry name" value="V-TYPE PROTON ATPASE PROTEOLIPID SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00137">
        <property type="entry name" value="ATP-synt_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00122">
        <property type="entry name" value="VACATPASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81333">
        <property type="entry name" value="F1F0 ATP synthase subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="8 10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <keyword evidence="4 10" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="29"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="61"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="103"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="V-ATPase proteolipid subunit C-like" evidence="11">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="domain" description="V-ATPase proteolipid subunit C-like" evidence="11">
        <location>
            <begin position="70"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7749125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="133" mass="13472" checksum="244D25288781368A" modified="2024-05-29" version="1">MGAAYGTAKSGVGVASMGVMRPELVMKSIVPVVMAGVLGIYGLIIAVIISTGINPKAKSYYLFDGYAHLSSGLACGLAGLSAGMATGIVGDAGVRANAQQPKLFVGMILILIFXXXXXXGIILSSRAGQSRAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GNY0</accession>
    <name>A0AAD5GNY0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_029552</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006819">
        <property type="protein sequence ID" value="KAI7747191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GNY0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00018">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.730.10:FF:000002">
        <property type="entry name" value="AP2-like ethylene-responsive transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.730.10:FF:000003">
        <property type="entry name" value="AP2-like ethylene-responsive transcription factor ANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.730.10">
        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001471">
        <property type="entry name" value="AP2/ERF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32467">
        <property type="entry name" value="AP2-LIKE ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32467:SF157">
        <property type="entry name" value="AP2-LIKE ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR CRL5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00847">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00367">
        <property type="entry name" value="ETHRSPELEMNT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00380">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51032">
        <property type="entry name" value="AP2_ERF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="8">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="AP2/ERF" evidence="8">
        <location>
            <begin position="94"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="224"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="239"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="252"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7747191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="288" mass="32568" checksum="E0E61A767546041A" modified="2024-05-29" version="1" fragment="single">WTGRYEAHLWDNSCKKEGQSRKGRQVYLGGYDMEEKAARAYDLAALKYWGPSTHINFPLENYEQEILEMKNMSRQEYVAHLRRRSSGFSRGASIYRGVTRHHQHGRWQARIGRVAGNKDLYLGTFSTQEEAAEAYDVAAIKFRGVNAVTNFDISRYNVKKIIESNTLLAGELARRTKDVDTTNEILSDQPAAETGEPKTMLTEYNTNRNMLDWKMSLYDQNQTRPGVDVNGSSKLGAHVSNDSSLVTSLSSSREDSPERNSSRETWIPTAAAAQMRAQIPVFAAWTDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GP20</accession>
    <name>A0AAD5GP20_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">glucan endo-1,3-beta-D-glucosidase</fullName>
            <ecNumber evidence="4">3.2.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_012863</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of (1-&gt;3)-beta-D-glucosidic linkages in (1-&gt;3)-beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.39"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the glycosyl hydrolase 17 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.39" evidence="4"/>
    <dbReference type="EMBL" id="JAMZMK010006605">
        <property type="protein sequence ID" value="KAI7748074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GP20"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042973">
        <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000005">
        <property type="entry name" value="Glucan endo-1,3-beta-glucosidase 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1040:FF:000003">
        <property type="entry name" value="glucan endo-1,3-beta-glucosidase 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000490">
        <property type="entry name" value="Glyco_hydro_17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044965">
        <property type="entry name" value="Glyco_hydro_17_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012946">
        <property type="entry name" value="X8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32227">
        <property type="entry name" value="GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00332">
        <property type="entry name" value="Glyco_hydro_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07983">
        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00768">
        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042034251" description="glucan endo-1,3-beta-D-glucosidase" evidence="12">
        <location>
            <begin position="28"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="domain" description="X8" evidence="13">
        <location>
            <begin position="383"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="348"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="365"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7748074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="471" mass="49789" checksum="558707658F1E2620" modified="2024-05-29" version="1">MALPTTSLHKSFILLTFLLHFSTFTSSESFIGVNYGIVANNLPAPEATAKLLESTSIGKVRMYGADPAIIRALAGTGIGIVIGAANGDIPALASDPNFATQWVNSNVLAYPDSNIIVITVGNEVMTAVDQSLVSLCLPAMQNVQNALNAASLGGKIKVSTVHSMAVLASSDPPSSAVFNSGFSDTMRSLLKFHSANGSPFMINPYPFFAYQSDTRPETLAFCLFQPNAGRVDSGNGIKYMNMFDAQVDAVRSALNAMGFKDVEIVVAETGWAYKGDPNEAGPSIENAKAYNGNLINHLRSKVGTPLMPGKTIDTYIFALYDENLKPGPGSERAFGLFKPDLSMTYDVGLSKNSQTPTTPTPTSPTTPTTPTTPATPSPVQTGGWCMPKPGVSDAQLQSNLDYVCSQGLDCGPLQPGGACFEPNTVASHAAYAMNLLYQTAGRNPWNCDFLQTATLTSNNPSYNGCVYPGGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GTA0</accession>
    <name>A0AAD5GTA0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_006048</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010003865">
        <property type="protein sequence ID" value="KAI7754360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GTA0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22160">
        <property type="entry name" value="F-box_AtFBL13-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053781">
        <property type="entry name" value="F-box_AtFBL13-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055411">
        <property type="entry name" value="LRR_FXL15/At3g58940/PEG3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31639:SF312">
        <property type="entry name" value="CYCLIN-LIKE F-BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31639">
        <property type="entry name" value="F-BOX PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24758">
        <property type="entry name" value="LRR_At5g56370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="21"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7754360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="411" mass="46562" checksum="2722EF6CC429BEA7" modified="2024-05-29" version="1">MQIIMELNHGTHKASKFAPDQDVISRLPDNVISNILNLLPLQDAVRTGILSRKWRFKWTMLTQLILDENLITYLIKVGQRNHFKIISRVLYLLSGAITKFVLFIDRYGYFSLDFEDVNHWLLFLSRKGIKDLTIVQRHKSIDKLPTHLFSCLELKHLKLSNCSFHPPTTFHGFPNLLSLELSQVQFARGKFGELFTLCPLLEILNMNATGVVKLDDIAKLANLKTLSLSMYCLENTTITSTSSTIFELLDFLPKLQELNLDFQRCKLIQGGAEKRFPTAFPSLKALKLSEIDFGSAIMLSCVSEMIRHLPNLQTLEIIAASVSSAIPTPTICFSEIDYNPVGSSQLQSAVFLDLKGSENEACLIKCLLKCSPFLKMIGIHLHRPLFDGHLMFATKLLKLKGSYPEVEINLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GTC6</accession>
    <name>A0AAD5GTC6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_027810</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the RNase T2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005955">
        <property type="protein sequence ID" value="KAI7751198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GTC6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033897">
        <property type="term" value="F:ribonuclease T2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01061">
        <property type="entry name" value="RNase_T2_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.730.10:FF:000003">
        <property type="entry name" value="Ribonuclease 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.730.10">
        <property type="entry name" value="Ribonuclease T2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033697">
        <property type="entry name" value="Ribonuclease_T2_eukaryotic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001568">
        <property type="entry name" value="RNase_T2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036430">
        <property type="entry name" value="RNase_T2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018188">
        <property type="entry name" value="RNase_T2_His_AS_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033130">
        <property type="entry name" value="RNase_T2_His_AS_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11240:SF82">
        <property type="entry name" value="RIBONUCLEASE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11240">
        <property type="entry name" value="RIBONUCLEASE T2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00445">
        <property type="entry name" value="Ribonuclease_T2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55895">
        <property type="entry name" value="Ribonuclease Rh-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00530">
        <property type="entry name" value="RNASE_T2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00531">
        <property type="entry name" value="RNASE_T2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042237516" evidence="9">
        <location>
            <begin position="26"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="63"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="117"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR633697-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7751198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="239" mass="26852" checksum="575F5A278F549A3B" modified="2024-05-29" version="1">MMKLNGRTLLVTFLFTQSVAILTVAKEFDFFYFVQQWPGSYCDSRRGCCYPKTGKPAEDFSIHGLWPNYVDGTYPSNCDSSNPFDDSKVSDLESVLQEHWPTLACPSGDGLKFWRHEWEKHGTCAESVFDERGYFEAALSLKKKANLLGALENAGIRPADEKFHTMGQIKDAITNAVGYEPFIECNVDSSGYHQLYQVYQCVDASASNFIKCPVLPHGRACGNKVEFPSFSSTMSRDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GUM6</accession>
    <name>A0AAD5GUM6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Thaumatin-like protein 1b</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_011530</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005575">
        <property type="protein sequence ID" value="KAI7752951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GUM6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09218">
        <property type="entry name" value="TLP-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.110.10:FF:000001">
        <property type="entry name" value="THAUMATIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.110.10">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037176">
        <property type="entry name" value="Osmotin/thaumatin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001938">
        <property type="entry name" value="Thaumatin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017949">
        <property type="entry name" value="Thaumatin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31048">
        <property type="entry name" value="OS03G0233200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00314">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00347">
        <property type="entry name" value="THAUMATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00205">
        <property type="entry name" value="THN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49870">
        <property type="entry name" value="Osmotin, thaumatin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00316">
        <property type="entry name" value="THAUMATIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51367">
        <property type="entry name" value="THAUMATIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042270730" description="Thaumatin-like protein 1b" evidence="2">
        <location>
            <begin position="29"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="248"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="248"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="286"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="305"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7752951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="331" mass="34332" checksum="EF80EDA1E91AB0B4" modified="2024-05-29" version="1" fragment="single">MDLLCSSFVTHFLTLFFAISLLPRGVFGATFTFVNKCNYTVWPGILANAGSPPLDTTGFELREQNSRSFPAPTGWSGRFWARTGCKFDRSGSGSCQTADCGSGQLECNGAGAAPPATLAEFTLGTTGGSDFYDVSLVDGYNIAMIVEVSGGSGLCATTGCVNDLNLQCPNELRVESGHGCKSACEAFGSPEYCCSGAYNTPATCKPSVYSQMFKMACPRSYSYAYDDPTSTFTCAGADYTVTFCPSMPSQKSSSDMSPPATTTTDGSTSTTLDEGDESIPGSVTESGYGYASLSGSGLDSEPDTGSGSVSGSESGTEALEANESWLAGLAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GVM8</accession>
    <name>A0AAD5GVM8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NIN-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_018917</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010002421">
        <property type="protein sequence ID" value="KAI7754824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GVM8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045012">
        <property type="entry name" value="NLP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32002:SF35">
        <property type="entry name" value="PROTEIN NLP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32002">
        <property type="entry name" value="PROTEIN NLP8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7754824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="247" mass="27959" checksum="1275BF07027BBB09" modified="2024-05-29" version="1">MIRMTDMAFSSESRYLNPLKVFVNDPRITDLELGPDKVDTDNQKIQDLEVGANKVGHKKQDKIIAALKLLTFRERHVLVQFWSACESGKNRLLTTTNQPFGLGVIDVGLRSYRKDSEGRSFSVDNDHEEEDCSPTSRVFGRGLPEWTSDLDNYTPKHFPQQEFAIRCNLHGYLALPVFDSATQLCVGVLELLTSSKYTSYAYEARQVHNALEVSILSLNCDNILFVTHQCLCSDLPLQVEPLRLNCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GWV3</accession>
    <name>A0AAD5GWV3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Alpha/beta hydrolase fold-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_021598</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDXG' lipolytic enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000656">
        <property type="protein sequence ID" value="KAI7755979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GWV3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050466">
        <property type="entry name" value="Carboxylest/Gibb_receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033140">
        <property type="entry name" value="Lipase_GDXG_put_SER_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23024">
        <property type="entry name" value="ARYLACETAMIDE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23024:SF577">
        <property type="entry name" value="CARBOXYLESTERASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01174">
        <property type="entry name" value="LIPASE_GDXG_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="3">
        <location>
            <begin position="74"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7755979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="334" mass="36774" checksum="1E60CEB3AA8493F2" modified="2024-05-29" version="1">MGSVSREILHDVPPYIRVYKDKTIERLLGTEVIPPTTDPITGVTSKDIIISPQTGITARIFRPSQTTNKKLPLIIYFHGGAFVIASPAEPKYHNSVTKLVSESRAIAVSVDYRLAPEHPLPTAFDDSFAALQWVSSHIVVGTGSGSGSGLEEWIKNDVDFDKVYLAGDSAGATIAHHLAIRVGSNPSIRPDPDVRFKIRGMILINPYFWGKDPIGSEKTDPIRKAMVDKWWEFVCPSGLDDGSSKLDDPLINPMVNGAPDLAGLGCGRVIITVSEKDILRDRGWAYHEALVKSNWNGNVEMVEIEGEDHVFHIFDPNGEKAMNMMKKLASFINE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GZ39</accession>
    <name>A0AAD5GZ39_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">4-coumarate--CoA ligase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_017475</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Phytoalexin biosynthesis; 3,4',5-trihydroxystilbene biosynthesis; 3,4',5-trihydroxystilbene from trans-4-coumarate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000255">
        <property type="protein sequence ID" value="KAI7756808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GZ39"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016405">
        <property type="term" value="F:CoA-ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009698">
        <property type="term" value="P:phenylpropanoid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.30:FF:000007">
        <property type="entry name" value="4-coumarate--CoA ligase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24096:SF425">
        <property type="entry name" value="4-COUMARATE--COA LIGASE-LIKE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24096">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0587">Phenylpropanoid metabolism</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="80"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="6">
        <location>
            <begin position="28"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="7">
        <location>
            <begin position="301"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7756808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="390" mass="43053" checksum="87A0049D278FC2AE" modified="2024-05-29" version="1">MFLGSDSPRKDCFCYKDLILLSGSVSELPNVEINGDHTAALLYSSGTTGVSKGVVLSHMNFITVSQMIVSDQRLMNGKDYVYLCFLPMFHIFGFGVVLYAQLQEGNTIVSMEKFDFVGMLKNIERYRVTHLWVVPPVFLALAKQDVVKKFDLSSLKQIGSGAAPLGKELMEECAKKLPHVVVLQGYGMTETTGVISVESPVMGPRHSGSAGRLISGVEAKIVSVDTCKPLPPNQTGEIWVRGANMMQGYFNNPQATKLTIDKQRFVHTGDLGYFDDEGQLYVVDRIKELIKVKGFQVAPAELEGLLVSHSEILDAAVIPFPDAEAGEVPMAFVVRSPKSLLTEEDVKKFISEQVAPYKRLKRVTFVNSVPKSAAGKLLRRELIEKVRSKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BKJ3</accession>
    <name>A0AAD5BKJ3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Sulfite exporter TauE/SafE family protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_006975</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the 4-toluene sulfonate uptake permease (TSUP) (TC 2.A.102) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012149">
        <property type="protein sequence ID" value="KAI7724861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BKJ3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031464">
        <property type="term" value="C:Cul4A-RING E3 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002781">
        <property type="entry name" value="TM_pro_TauE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14255">
        <property type="entry name" value="CEREBLON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14255:SF5">
        <property type="entry name" value="SULFITE EXPORTER TAUE_SAFE FAMILY PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01925">
        <property type="entry name" value="TauE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="63"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="161"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="303"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="326"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="377"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="415"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="445"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="476"/>
            <end position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7724861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="519" mass="57491" checksum="8E2D58EFADCFFE2F" modified="2024-05-29" version="1">MATKTFVLYLLACSSAAVLTALLISTDHTHKTNNIIINRHPSNHGILNTDEKTWPELAFNWRIMLATLIGFLGSAFGTVGGVGGGGIFVPMLTLVVGFDTKSAAALSKCMIMGASASSFWYNLRVQHPCREVPILDYDLALLFQPMLMLGITLGVALSVVFPYWLITVLIIILFLGFTTKQLSPRSGRRLDLKSHITVLFGLTDDSANRSKTTNLGVTRGIEMWKEESILKKEMARPRETFVNSRGELKIPMVGDISWDIRPWVKYFTDGSTTVLIDAFDPLIPKEEKTQMEVFTFNLRWKKLMVLVAVWAAFLVLQNDLVACSTWYWVLTVAQFPATLAVFGFECIRLYKESKKRKSLGNTESVCEATINWTVPNLIFCALCGILGGTVGGLLGSGGGFILGPLLLEIGVIPQVASATATFVMMFSSSLSVVEFYLLKRFPIPYALYLMSVSVLAGFWGQFFVRKLVVFLKRASIIVFILSAVIFASALTMGVIGIEQSIRMIHNHEFMGFLDFCSNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BKQ1</accession>
    <name>A0AAD5BKQ1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phytosulfokine</fullName>
        </recommendedName>
        <component>
            <recommendedName>
                <fullName evidence="9">Phytosulfokine-alpha</fullName>
                <shortName evidence="9">PSK-alpha</shortName>
                <shortName evidence="9">Phytosulfokine-a</shortName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName evidence="9">Phytosulfokine-beta</fullName>
                <shortName evidence="9">PSK-beta</shortName>
                <shortName evidence="9">Phytosulfokine-b</shortName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_019153</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Promotes plant cell differentiation, organogenesis and somatic embryogenesis as well as cell proliferation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="9">PSK-alpha is produced by endopeptidase digestion. PSK-beta is produced from PSK-alpha by exopeptidase digestion.</text>
    </comment>
    <comment type="PTM">
        <text evidence="9">Sulfation is important for activity and for the binding to a putative membrane receptor.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the phytosulfokine family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012076">
        <property type="protein sequence ID" value="KAI7725055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BKQ1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008283">
        <property type="term" value="P:cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009438">
        <property type="entry name" value="Phytosulfokine"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33285">
        <property type="entry name" value="PHYTOSULFOKINES 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33285:SF55">
        <property type="entry name" value="PHYTOSULFOKINES 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06404">
        <property type="entry name" value="PSK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7 9" id="KW-0221">Differentiation</keyword>
    <keyword evidence="8 9" id="KW-0339">Growth factor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 9" id="KW-0732">Signal</keyword>
    <keyword evidence="5 9" id="KW-0765">Sulfation</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041770311" description="Phytosulfokine" evidence="9">
        <location>
            <begin position="27"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU368031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7725055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="86" mass="9663" checksum="761E6E45AF45AC97" modified="2024-05-29" version="1">MSKAIKVTTMCTIALLLLSSLSYAVARLPPSVNTVPPVQTHQLDEEIANGYVELNCKGDDDEEEECLRRRTLVAHLDYIYTQNKQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BLZ1</accession>
    <name>A0AAD5BLZ1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_016619</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Probable transcription factor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011850">
        <property type="protein sequence ID" value="KAI7725827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BLZ1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00265">
        <property type="entry name" value="MADS_MEF2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1810.10:FF:000003">
        <property type="entry name" value="MADS-box transcription factor MADS-MC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1810.10">
        <property type="entry name" value="Transcription factor, MADS-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050142">
        <property type="entry name" value="MADS-box/MEF2_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033896">
        <property type="entry name" value="MEF2-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002487">
        <property type="entry name" value="TF_Kbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002100">
        <property type="entry name" value="TF_MADSbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036879">
        <property type="entry name" value="TF_MADSbox_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48019">
        <property type="entry name" value="SERUM RESPONSE FACTOR HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01486">
        <property type="entry name" value="K-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00319">
        <property type="entry name" value="SRF-TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00404">
        <property type="entry name" value="MADSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00432">
        <property type="entry name" value="MADS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55455">
        <property type="entry name" value="SRF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51297">
        <property type="entry name" value="K_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00350">
        <property type="entry name" value="MADS_BOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50066">
        <property type="entry name" value="MADS_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MADS-box" evidence="8">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="K-box" evidence="9">
        <location>
            <begin position="85"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="119"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7725827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="250" mass="28921" checksum="D793C0B7C6F32C51" modified="2024-05-29" version="1" fragment="single">MGRGKIEVKLIENTTSRQVTFSKRRTGLIKKTHELSVLCDAQIGLIVFSSKGKLYEYTTHPLSMTQMIDKYLKATGSSLTQQTNQEQMRSELSRMKKETLNLQLSLQRYKGDDLSSAQIEELNQLEQQLEFSVQKVRARKLQLLQQQVDNLQRTEKVLEKENEEMYQWLMGKQQEEINRQQVSAMTELKLVGQEHQLFENFPFYGTEEPPNSALQLAATLPQLQLQSYPYHLQPAQPNLQESSSAQQINI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BME4</accession>
    <name>A0AAD5BME4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Bidirectional sugar transporter SWEET</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_014234</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Mediates both low-affinity uptake and efflux of sugar across the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the SWEET sugar transporter family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012062">
        <property type="protein sequence ID" value="KAI7725091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BME4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051119">
        <property type="term" value="F:sugar transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.290:FF:000001">
        <property type="entry name" value="Bidirectional sugar transporter SWEET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.290:FF:000002">
        <property type="entry name" value="Bidirectional sugar transporter SWEET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.290">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047664">
        <property type="entry name" value="SWEET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004316">
        <property type="entry name" value="SWEET_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10791:SF143">
        <property type="entry name" value="BIDIRECTIONAL SUGAR TRANSPORTER SWEET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10791">
        <property type="entry name" value="RAG1-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03083">
        <property type="entry name" value="MtN3_slv"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="71"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="133"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="164"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="192"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU910715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7725091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="245" mass="27097" checksum="4063B896AB26F5FC" modified="2024-05-29" version="1">MVSEETVRTALGVAGNITAVTLFLSTVPTFYRIWKKGSVEQYSPMPYLASFFNCGLWVLYGMPFVHPNSLLVVTTNGVGLVIEIGYLTIYLVCSEPRKRLQVALMVLLEVLVFSGIVLLVLTMVHTTNKRSTIVGSISVVSTILMYASPLSVMKLVITTKSVEYMPFLFSLFCFCNGLCWFVYALFPFDPFIAVPNGLGALFGILQLILYATFYKSTKPMLAARKEKPDLGLAVMTMHNGNDARA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BMI1</accession>
    <name>A0AAD5BMI1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cytochrome b5 heme-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">M8C21_004732</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">AA19_3_7</strain>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Microsome membrane</location>
            <topology evidence="12">Single-pass membrane protein</topology>
            <orientation evidence="12">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13 14">Belongs to the cytochrome b5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011765">
        <property type="protein sequence ID" value="KAI7726061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BMI1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.120.10:FF:000002">
        <property type="entry name" value="Cytochrome b5 type B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018506">
        <property type="entry name" value="Cyt_B5_heme-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050668">
        <property type="entry name" value="Cytochrome_b5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19359">
        <property type="entry name" value="CYTOCHROME B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19359:SF129">
        <property type="entry name" value="CYTOCHROME B5 ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00363">
        <property type="entry name" value="CYTOCHROMEB5"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00191">
        <property type="entry name" value="CYTOCHROME_B5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3 14" id="KW-0349">Heme</keyword>
    <keyword evidence="10 14" id="KW-0408">Iron</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0492">Microsome</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="104"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="15">
        <location>
            <begin position="2"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU362121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAI7726061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="146" mass="16359" checksum="8A2B34D943293353" modified="2024-05-29" version="1" fragment="single">MGKVFTLKEVADHNTPKDCWLIIDGKVFDVTKFLEDHPGGDDVLLSATGKDATDDFEDVGHSTTAKSMMDEFYVGDIDASTIPSKVEYKAPNQAHYNQDKTPEFIIKILQFLVPLVILGVAVGIRFYTKALILKLFDLRDDNNDMM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BMJ3</accession>
    <name>A0AAD5BMJ3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_031017</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase A1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011744">
        <property type="protein sequence ID" value="KAI7726137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BMJ3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032799">
        <property type="entry name" value="TAXi_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13683">
        <property type="entry name" value="ASPARTYL PROTEASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13683:SF800">
        <property type="entry name" value="EUKARYOTIC ASPARTYL PROTEASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14541">
        <property type="entry name" value="TAXi_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase A1" evidence="2">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7726137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="127" mass="14287" checksum="9A4CE958DD590391" modified="2024-05-29" version="1" fragment="single">VIKELSGKPVKEAVDDKTLPFCWKGRKPFRSIRDVSKYFKPLALRFSSGWRSKTQFEIPLEGYLLISSKGNVCLGVLNGTEVGLQDFNIIGDISMQDKMVIYDNEKQTIGWTPANCDRLPSSDTISI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BN85</accession>
    <name>A0AAD5BN85_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_025515</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011980">
        <property type="protein sequence ID" value="KAI7725299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BN85"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7725299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="18" mass="2100" checksum="E4166C2FF4F0D804" modified="2024-05-29" version="1" fragment="single">MLMIRLHMRQMGEVAGIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BP56</accession>
    <name>A0AAD5BP56_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_002333</name>
        <name evidence="1" type="ORF">M8C21_013234</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011818">
        <property type="protein sequence ID" value="KAI7725946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAMZMK010006763">
        <property type="protein sequence ID" value="KAI7747412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BP56"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7725946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7747412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="70" mass="7981" checksum="966CCF1321FF3BA0" modified="2024-05-29" version="1">MTKALPLLRYSVLVISSAVATNNIPSARIARVFQLLFSLKLPVFATPLRLQIRYRVARYKFGEENQCLNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BPU7</accession>
    <name>A0AAD5BPU7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Membrane insertase YidC/Oxa/ALB C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_027582</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the OXA1/ALB3/YidC (TC 2.A.9.2) family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the OXA1/ALB3/YidC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011403">
        <property type="protein sequence ID" value="KAI7727310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BPU7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032977">
        <property type="term" value="F:membrane insertase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051205">
        <property type="term" value="P:protein insertion into membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072598">
        <property type="term" value="P:protein localization to chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010027">
        <property type="term" value="P:thylakoid membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20070">
        <property type="entry name" value="5TM_YidC_Alb3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001708">
        <property type="entry name" value="YidC/ALB3/OXA1/COX18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028055">
        <property type="entry name" value="YidC/Oxa/ALB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047196">
        <property type="entry name" value="YidC_ALB_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03592">
        <property type="entry name" value="yidC_oxa1_cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12428:SF14">
        <property type="entry name" value="ALBINO3-LIKE PROTEIN 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12428">
        <property type="entry name" value="OXA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02096">
        <property type="entry name" value="60KD_IMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00701">
        <property type="entry name" value="60KDINNERMP"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="133"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="192"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="238"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="280"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="Membrane insertase YidC/Oxa/ALB C-terminal" evidence="9">
        <location>
            <begin position="135"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="340"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="412"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="351"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="412"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="448"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="473"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7727310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="482" mass="53609" checksum="086E75A40C7A8A45" modified="2024-05-29" version="1">MANSISFGSKSVLSPFPDPTRTRPINPFIRRTQFVNFNQNHKVLFRGSTLTVARFGSGHVQFPDPENFRELINRAEGLIYTIADAVVAANPDSDVTTNVATKQSSDWLSGITNYMETVLKFLKDGLSTLHVPYAYGFAIILLTVLVKAATFPLSKKQVESAMAIRSLQPQIKAIQERYAGDQERLQLETARLYKLAGINPLAGCLPTLATIPVWIGLYRALSNVADEDGHPPLGWSDTFAYLVLPVLLVISQYISVQIMQSSQPQSNDPNMKTSQTITKFLPLMIGYFALSVPSGLSLYWLTNNILTSAQQIWLQKMGGANNPAKKINYEMLKEDQPKVQESVFQTTSVKEPPKIEKPDSQGYRPGERFKQLKEQEARRKQQKEAEKAKEASLANEAVKGDIKVDIELKADSLNSASNGSPSSSDSSQVDKSLISKMENGEDSALYNSRRDQQHLDENLEKEKTATCTTENKSATKDEHQEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BRC5</accession>
    <name>A0AAD5BRC5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_007875</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011457">
        <property type="protein sequence ID" value="KAI7727043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BRC5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7727043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="268" mass="31107" checksum="933AB01418F58D3B" modified="2024-05-29" version="1">MCFEDATMESHKIEAILYEPLPVLPWKIRDHDYQLPFCKLVSNMKKLRWLSVSTMGQDKDEGPNFLSNELRYIEWYSYPAISPFLNGFLPVKLVVLKLTDCKQKELWKGYQLCQVSLIDGLSDRLLQFMLKGKAIENGSMRLQLEGLEIPKGLTPPLLRGNRCRLELPENWCNDYSGFLICVVTSYDYFAIPYRIWISMKQVSNNVSEDDVIWEERRGDEYMTWVWGFGVRLVEKKSGSGLTETSRYSSHHTPMIKIQHDSASTLTIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BSF6</accession>
    <name>A0AAD5BSF6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">HMA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_028676</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the HIPP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011152">
        <property type="protein sequence ID" value="KAI7728747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BSF6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009626">
        <property type="term" value="P:plant-type hypersensitive response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.100:FF:000035">
        <property type="entry name" value="Heavy metal-associated isoprenylated plant protein 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22814">
        <property type="entry name" value="COPPER TRANSPORT PROTEIN ATOX1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22814:SF370">
        <property type="entry name" value="HEAVY METAL-ASSOCIATED DOMAIN, HMA, HEAVY METAL-ASSOCIATED DOMAIN SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0104">Cadmium</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0488">Methylation</keyword>
    <keyword evidence="6" id="KW-0636">Prenylation</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMA" evidence="8">
        <location>
            <begin position="14"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7728747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="137" mass="15445" checksum="704617382C4A4752" modified="2024-05-29" version="1">MLYLTKMGRGYFVSQTVEIKVKMDCDGCERRVKNSVKSMKGVKTVEINRKQSRVTVSGNVDPNKVLKNVKSTGKRAEFWPYIPYNLVSYPYVSQAYDKRAPAGHVKNVVQAVAAHNTTDEHIAHFFSDDNPNACSIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BTY3</accession>
    <name>A0AAD5BTY3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_010250</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011006">
        <property type="protein sequence ID" value="KAI7729412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BTY3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7729412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="95" mass="10556" checksum="4D773EFBD8EF089F" modified="2024-05-29" version="1" fragment="single">NILIERDDKVWHQQTTESSLHIHQRDAVFSFTKQISESVGALVESPETLSICFMSSTIHIFSSNSLTTSIVGMSGFEFSSTETLESTKRSLLESA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BUA5</accession>
    <name>A0AAD5BUA5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_003072</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010925">
        <property type="protein sequence ID" value="KAI7729766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BUA5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="124"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="184"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="124"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7729766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="339" mass="38402" checksum="144EBE030435951A" modified="2024-05-29" version="1" fragment="single">LRVWDVILFEGNRSMLLRTALALMELYGPELGNAMDAGDAIILLQSLLGSTFDSSNLVVTACISFMDVTEDTLRELRDKHRPDVITAVEERIKGEHLRKSSKGLANKLYSFKHEREPLTRELSVKPGATREKRLDKDSSDLMSPLKRDNPFNTEPINAEVDSLPDIQDQVIWLKAQLCSMLEDKRSATVRAEELETALMEMAQQDNRLELSAKIELMEQDLFELQQLLSDKKEQEKAMLEVLLKVEQDQKITEDARISAERDAAAQKYLKKYEEAMVSLAHMEKRVVMAETTLEATLQYNYGLVKAQATPAGNTTTKTRTGILSFGLGWRDKNKVSKHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BUJ9</accession>
    <name>A0AAD5BUJ9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ubiquitin thioesterase OTU</fullName>
            <ecNumber evidence="3">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_031307</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Hydrolase that can remove conjugated ubiquitin from proteins and may therefore play an important regulatory role at the level of protein turnover by preventing degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010010878">
        <property type="protein sequence ID" value="KAI7729948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BUJ9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030968">
        <property type="term" value="P:endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003323">
        <property type="entry name" value="OTU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13312">
        <property type="entry name" value="HIV-INDUCED PROTEIN-7-LIKE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13312:SF0">
        <property type="entry name" value="UBIQUITIN THIOESTERASE OTU1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50802">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="OTU" evidence="4">
        <location>
            <begin position="5"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU367104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7729948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="150" mass="17181" checksum="18FF855D354ABD7B" modified="2024-05-29" version="1" fragment="single">MEGSIVRRVIPSDNSCLFNAVGYVMDHDKHKAAELRQVIAATVASDPTRYSEAFLGKPNEEYCAWILNPEKWGDYYGREIAAYDIQTTRCDLYGQEKKYGERVMLIYDGLHYDALAVCHSLSLLFLPLHLHTLRQWICFWVGALHLGLNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BUR6</accession>
    <name>A0AAD5BUR6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_002211</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010011186">
        <property type="protein sequence ID" value="KAI7728614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BUR6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056760">
        <property type="entry name" value="RING_XB3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24128:SF14">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE XBAT31-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24128">
        <property type="entry name" value="HOMEOBOX PROTEIN WARIAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24921">
        <property type="entry name" value="RING_XB3-XBAT31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10 11" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="repeat" description="ANK" evidence="11">
        <location>
            <begin position="46"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="11">
        <location>
            <begin position="80"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="11">
        <location>
            <begin position="114"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="11">
        <location>
            <begin position="159"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="11">
        <location>
            <begin position="196"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="13">
        <location>
            <begin position="324"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7728614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="447" mass="48236" checksum="1C26EAB1D27BA9E0" modified="2024-05-29" version="1">MGQGLSCRVVQDEHGLFTAVQYGHLDIVNNLLLKNPSLIHQTTVYDRHSALHIAAANAQIEIVCLLLKNGSVNPDCLNRHKQTPLMLAAMHGKIACVEKLIEAKANILMFDSLNGRTCLHYAAYYGHSDCLEAILSAARTSHVAASWGFSRFVNIRDGKGATPLHLASRQRRSQCVHMLLDNGALVSASTGGYGFSGSTPLHLAARGGSIDCIRELLAWGADRLHRDASGRIPYLVALKHKNEACAALLNPSSAAPLVWPSPLKFISELNQDAKALLEQALMEANRAREKTILKGAGYSLQSPSHSDTGTADDNISEASETELCYICFDQVCTIEVQDCGHQMCAQCTLALCSHNKPDPTTASLTAPVCPFCRSNIARLVVAKVKVNDFDNDLDLYPSCKLPKLRKARNFSEGSSSFKGLSGVSSFGKMVGRGSGKVSVDSECIDKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BV48</accession>
    <name>A0AAD5BV48_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent)</fullName>
            <ecNumber evidence="5">5.4.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_013488</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>O-phospho-L-seryl-[protein] + alpha-D-glucose 1-phosphate = alpha-D-glucose 1,6-bisphosphate + L-seryl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:68748"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:58392"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>alpha-D-glucose 1,6-bisphosphate + L-seryl-[protein] = O-phospho-L-seryl-[protein] + alpha-D-glucose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:68752"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:58225"/>
            <dbReference type="ChEBI" id="CHEBI:58392"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>alpha-D-glucose 1-phosphate = alpha-D-glucose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:23536"/>
            <dbReference type="ChEBI" id="CHEBI:58225"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="EC" id="5.4.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.2" evidence="5"/>
    <dbReference type="EMBL" id="JAMZMK010010907">
        <property type="protein sequence ID" value="KAI7729839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BV48"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009570">
        <property type="term" value="C:chloroplast stroma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004614">
        <property type="term" value="F:phosphoglucomutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004615">
        <property type="term" value="F:phosphomannomutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03089">
        <property type="entry name" value="PMM_PGM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.120.10:FF:000014">
        <property type="entry name" value="Phosphomannomutase/phosphoglucomutase isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.120.10:FF:000010">
        <property type="entry name" value="phosphomannomutase/phosphoglucomutase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.120.10:FF:000022">
        <property type="entry name" value="phosphomannomutase/phosphoglucomutase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.120.10">
        <property type="entry name" value="Alpha-D-Glucose-1,6-Bisphosphate, subunit A, domain 3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005844">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016055">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I/II/III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005845">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005846">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005841">
        <property type="entry name" value="Alpha-D-phosphohexomutase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050060">
        <property type="entry name" value="Phosphoglucosamine_mutase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42946:SF2">
        <property type="entry name" value="PHOSPHOGLUCOMUTASE (ALPHA-D-GLUCOSE-1,6-BISPHOSPHATE-DEPENDENT)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42946">
        <property type="entry name" value="PHOSPHOHEXOSE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02878">
        <property type="entry name" value="PGM_PMM_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02879">
        <property type="entry name" value="PGM_PMM_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02880">
        <property type="entry name" value="PGM_PMM_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00509">
        <property type="entry name" value="PGMPMM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53738">
        <property type="entry name" value="Phosphoglucomutase, first 3 domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="6" id="KW-0313">Glucose metabolism</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="11">
        <location>
            <begin position="72"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="12">
        <location>
            <begin position="264"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="13">
        <location>
            <begin position="355"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7729839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="507" mass="55262" checksum="5D009F8DB9402BA4" modified="2024-05-29" version="1" fragment="single">MSSTLVPSTSLNNNTPINHNFPSSCTPRTLLNTLNFPFSIPSKLTLISPKSSTTANYNDIGLDEEMGQIKRLQNGSDVRGVAVEGEPGRKVNLTPPAVEAIAESFGEWVMDGLVKEKGRLTEDVRVSLGRDPRISGGSLSVAIFSGLGRAGCITYDMGLATTPACFMSTVLAPFSYDASIMMTASHLPYTRNGLKFFTKKGGLTSPEVEDICNKAAFKYANRLTKVSTLLKTTPTRVDFMSVYAKHLRDIIKERVNHPQHYDTPLEGFQIIVNAGNGSGGFFTFDVLDKLGADTFGSLNLTPDGMFPNHIPNPEDKVAMSVTRTAVLETSADLGIVFDTDVDRSGVVDKEGNPINGDKLIALMAAIVLKEHPASTIVTDARTSMALTRFITDRGGHHCLYRVGYRNVIDKGVQLNEDGVDAHLMMETSGHGALKENYFLDDGAYMVVKIIIEMVRMKLEGSNEGIGSLIKDLEDPLESVELRMDILSEPKHAKAQAIEIIETFKTYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BWD9</accession>
    <name>A0AAD5BWD9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">28S ribosomal protein S34, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_000986</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010939">
        <property type="protein sequence ID" value="KAI7729698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BWD9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032053">
        <property type="entry name" value="Ribosomal_mS34"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35316">
        <property type="entry name" value="28S RIBOSOMAL S34 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35316:SF1">
        <property type="entry name" value="28S RIBOSOMAL S34 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16053">
        <property type="entry name" value="MRP-S34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="29"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7729698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="149" mass="16391" checksum="E30D4DC2B31EF3D9" modified="2024-05-29" version="1">MNTATRSVASIVKSWNPLSFTLTKRTLTTSSSSPASASNPSTTVAKSKRKNKKKNLFEVAQFLPNWGIGYQLAKTHWSGVAYQITKINLYKDGKHGKAWGIVHKDGVPAADAPKKISGVHKRCWKYIPNPKKTEQITSQSLPEPEVQAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BXD3</accession>
    <name>A0AAD5BXD3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_006553</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010526">
        <property type="protein sequence ID" value="KAI7731145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BXD3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="58"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7731145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="83" mass="9479" checksum="2470A4D73D455AD2" modified="2024-05-29" version="1" fragment="single">FIGYVQLCNDLDPVTKRNGIDTPKINFKLRDLDGVEINASLWEQHVVAMSTFMNDKNRDPHVTTASLTTLKEQELSSTQMLKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BY02</accession>
    <name>A0AAD5BY02_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Auxin-responsive protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_019556</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Aux/IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimers and heterodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Aux/IAA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010774">
        <property type="protein sequence ID" value="KAI7730476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BY02"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009734">
        <property type="term" value="P:auxin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000078">
        <property type="entry name" value="Auxin-responsive protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033389">
        <property type="entry name" value="AUX/IAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003311">
        <property type="entry name" value="AUX_IAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053793">
        <property type="entry name" value="PB1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31734:SF104">
        <property type="entry name" value="AUXIN-INDUCED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31734">
        <property type="entry name" value="AUXIN-RESPONSIVE PROTEIN IAA17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02309">
        <property type="entry name" value="AUX_IAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51745">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0927">Auxin signaling pathway</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0678">Repressor</keyword>
    <keyword evidence="5 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 8" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="PB1" evidence="10">
        <location>
            <begin position="147"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="94"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="98"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7730476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="272" mass="30100" checksum="89B1E54473CE2449" modified="2024-05-29" version="1">MITNMEKQAKAYEIMTNNKDLNLKATELRLGLPGSESPEREVVVHGSAIKILVAGAKRGFSDAINGGYGVGLVKNGGQETDVLVTPKPALLEKKTQVSDKLNGQSSSPAPKQQVVGWPPIRSFRKNTMAVSHTKNKEEDDPKIGSGCLYVKVSMDGAPYLRKVDLEIYSCYLDLSSALEKMFSCFTIGQYGSQEVRTREGLSETRLMDLLHGSEYVLTYEDKDGDWMLVGDVPWDMFIASCKRLRIMKSSDAIGLAPRAMEKSRNRTEPNNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BY75</accession>
    <name>A0AAD5BY75_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">beta-galactosidase</fullName>
            <ecNumber evidence="3">3.2.1.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_023052</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.</text>
            <dbReference type="EC" id="3.2.1.23"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the glycosyl hydrolase 35 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.23" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010010682">
        <property type="protein sequence ID" value="KAI7730688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BY75"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004565">
        <property type="term" value="F:beta-galactosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22842">
        <property type="entry name" value="Gal_Rha_Lectin_BGal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000142">
        <property type="entry name" value="Beta-galactosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041392">
        <property type="entry name" value="GHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031330">
        <property type="entry name" value="Gly_Hdrlase_35_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001944">
        <property type="entry name" value="Glycoside_Hdrlase_35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000922">
        <property type="entry name" value="Lectin_gal-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043159">
        <property type="entry name" value="Lectin_gal-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23421">
        <property type="entry name" value="BETA-GALACTOSIDASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17834">
        <property type="entry name" value="GHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01301">
        <property type="entry name" value="Glyco_hydro_35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02140">
        <property type="entry name" value="SUEL_Lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00742">
        <property type="entry name" value="GLHYDRLASE35"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50228">
        <property type="entry name" value="SUEL_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042242437" description="beta-galactosidase" evidence="8">
        <location>
            <begin position="19"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="domain" description="SUEL-type lectin" evidence="9">
        <location>
            <begin position="622"/>
            <end position="708"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7730688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="708" mass="78061" checksum="583D095447BD86C5" modified="2024-05-29" version="1">MMRLLIVWFLVVVAKVTAVNVTYDHRALIIDGRPRVLVSGSIHYPRSTPDMWPGLIKKSKEGGLDVIETYIFWNLHEPVRNQIENEYGNIDSPYGPAAKSYIKWAASMATSLDTGVPWVMCQQRDAPDPIIDTCNAFYCDGFKPNAENKPTMWTENWTGWFLLFGEAAPYRPVEDIAFAVTRFYQRGGTFQNYYMYHGGTNFGRTTGGPFIATSYDYNAPLDEYGTPRQPKWGHLKDLHQAIKLCEDALVATKPTTTSLGQHLEATVYKTSSTCAAFLANVDTKKDANVNFNGNYYHLPAWSVSILSDCKNLVFNTAKINSMATITRFVATSVRHDLSDSMAISSKWSYVSEPVGISSNDAFNRPGLVEQINTTADQSDYLWYSLSIDIKGAEPFLHDGFQTTLHVKSFGRVLHLFVNGQLVGSANGNMFTHGFTKNFSVVLKRGKNKLNLLSLTVGLQHFGAFFDKVGAGVTGPVKLEGLTNESTVDLSSQRWTYQIGLKGESLGLNTGGSSLWVLGSSKSQPLTWYKEWGKARRGSMVKVLGVIGQVILLKPAVVQYHVPRSWLKPSANVLVMFEEIGGDPTQISFKTQKLESLCSQVSESHPLPMEAWSQDNPTRKSKSMAKPRVLLECPDPNQVISSIKFASFGTPQGKCGSFSHGYCNSSNALTITQKACIGSRTCNIEVSSSTFGDPCVGVVKSLVVEASCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BYK0</accession>
    <name>A0AAD5BYK0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF2470 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_025248</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010399">
        <property type="protein sequence ID" value="KAI7731715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BYK0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="entry name" value="Electron Transport, Fmn-binding Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.180.10">
        <property type="entry name" value="PNP-oxidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019595">
        <property type="entry name" value="DUF2470"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037119">
        <property type="entry name" value="Haem_oxidase_HugZ-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13343">
        <property type="entry name" value="CREG1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13343:SF31">
        <property type="entry name" value="FMN-BINDING SPLIT BARREL, HEME OXYGENASE HUGZ-LIKE SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10615">
        <property type="entry name" value="DUF2470"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF2470" evidence="1">
        <location>
            <begin position="162"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7731715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="219" mass="24957" checksum="31D2D339E4BB730A" modified="2024-05-29" version="1">MSSPTQIPSQSHDDDDEVLKLIIAHQEKAARLPPVEEVKTILNHSTRGVLSTFSQDLLANPKCSLLVAKDTEDRTDLIITLLGDAVIVPENERDVVRTTYMARHPDAVWVDFDDFMFLRIEPKVVRYVYGVATDLFQSGEFTNNEFREAKVDQIYQFSKPISVDFAYMLDVDSLGFNAKAGYKDKNFKLRIAFPRRAEERKDVKTLIIEMLQAAKSRGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C0Y2</accession>
    <name>A0AAD5C0Y2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UspA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_030179</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010344">
        <property type="protein sequence ID" value="KAI7731991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C0Y2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23659">
        <property type="entry name" value="USP_At3g01520-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31964">
        <property type="entry name" value="ADENINE NUCLEOTIDE ALPHA HYDROLASES-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31964:SF122">
        <property type="entry name" value="OS02G0760500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="2">
        <location>
            <begin position="31"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7731991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="207" mass="22580" checksum="6851388AB0755152" modified="2024-05-29" version="1">MGQASSQLCVKTSSMSPQRRGRMGEKQEQLRKVVVAVDGSEESMKGLQWALDNVRLHPTEGSLVIIHVQSPPSISAGLNPGSIPFGAGPSDVEVPAFTAAIEAHQRRITEAIITHAFNICQHNNYEGEVKTQVVIGDPKDKICEAVEDLHADLLVMGCRSFGPIKSFNGGFWMTYESINNLLYIVCTLQALLFSPNIRVSISVVFDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C2Z4</accession>
    <name>A0AAD5C2Z4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">COP1-interacting protein 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_015035</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009846">
        <property type="protein sequence ID" value="KAI7734055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C2Z4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009416">
        <property type="term" value="P:response to light stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31008:SF4">
        <property type="entry name" value="COP1-INTERACTING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31008">
        <property type="entry name" value="COP1-INTERACTING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="105"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="261"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="328"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="475"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="668"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="125"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="269"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="331"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="348"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="376"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="406"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="424"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="504"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="699"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="743"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="785"/>
            <end position="801"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7734055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="801" mass="89698" checksum="FFB4C7AFE29A633E" modified="2024-05-29" version="1">MDPFIPLEYALFQLTPTRTRCDLLLCYGKNKEKLASGLVEPFISHLKLFKDQISKGGYSITLRPPTTNAFWFTKSTFQRLVRFINSPEVLERFIRLEREISQIENSNNESEEQAGEVGSLSSDGSTKKSTEPSKIKAGEAGDNAQEENSKVQLQRLLETRKAMLRKEQAMAYHRALVAGFETENMNDLILFADHFGASRLREACIEFRDLCKKKHNDGLWMDELAAMAAYPPSELAYTGTSGILIATESNASTLNNELNFEQEGDKNTTDQVPSTPSNVPMQMPWPNQMPQYMYNFPGPQGPRYPYPYPGMPPYYPAHMNWPANADDGYGRHHRSSSKKKSKSLLPDSSEEEDEDSNEEGESDSVSDSGTVSKHEKSSKKNKKKSSKTVVIRNINYITSNRKNSDGSDDSAEDEVDGVLESLVKHHSKSSKSHEKKKKDVDLDNTNSGKRNENWDAFQNLLLKDDDQDEHFANRNDAMDVGSESYVKNQPKARANDSFVVPDRNGFDESRKVSSHDFENGETFRMKGRDSSDADLVVSHRVGGGTDGMTNVRDLGSESSMIRNKRDGDWFVVKSSGSPVEKSTFSDDYASMRGDVFSSETNKKNEPIDDSFMVQPQTALPYDSEWRSTDVSIVESINKPDPNESSQAAKSGFYEPDDLYLMVSRDSVVEPSVRSSWTPEIDYGTEAQFTKSEPKPAPVETKEQTEESPVKAKTKKVAKPRPLSRSLPTDKRLPFVSRSVVPKSKREQEDEIRKRVEELAAERQRRIAERTAASASGSAKKPVIQKGSSGTPKKATTKATGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C2Z7</accession>
    <name>A0AAD5C2Z7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_020641</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the DnaJ family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009996">
        <property type="protein sequence ID" value="KAI7733433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C2Z7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031072">
        <property type="term" value="F:heat shock protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10747">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10719">
        <property type="entry name" value="DnaJ_zf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.260.20:FF:000005">
        <property type="entry name" value="Chaperone protein dnaJ 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000037">
        <property type="entry name" value="Chaperone protein dnaJ A6 chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.230.10:FF:000002">
        <property type="entry name" value="Molecular chaperone DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.260.20:FF:000009">
        <property type="entry name" value="Putative Mitochondrial DnaJ chaperone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.230.10">
        <property type="entry name" value="Heat shock protein DnaJ, cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.260.20">
        <property type="entry name" value="Urease metallochaperone UreE, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01152">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012724">
        <property type="entry name" value="DnaJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002939">
        <property type="entry name" value="DnaJ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008971">
        <property type="entry name" value="HSP40/DnaJ_pept-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001305">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036410">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02349">
        <property type="entry name" value="DnaJ_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008035">
        <property type="entry name" value="PRK10767.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43096:SF10">
        <property type="entry name" value="CHAPERONE PROTEIN DNAJ A6, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43096">
        <property type="entry name" value="DNAJ HOMOLOG 1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01556">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00684">
        <property type="entry name" value="DnaJ_CXXCXGXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57938">
        <property type="entry name" value="DnaJ/Hsp40 cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49493">
        <property type="entry name" value="HSP40/DnaJ peptide-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51188">
        <property type="entry name" value="ZF_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="4 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0934">Plastid</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="7 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="J" evidence="12">
        <location>
            <begin position="84"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="CR-type" evidence="13">
        <location>
            <begin position="214"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="CR-type" evidence="11">
        <location>
            <begin position="214"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00061004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7733433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="445" mass="47510" checksum="D795DF609BE80FFF" modified="2024-05-29" version="1">MAIVPGGSTWVARWGIQPQLMSRSSSASGSLMHSPRFSVNTKELASPCSAFFSQGSLHELYNFGSSGKSHHRSGGRLIVRAESDYYSVLGVSKNASKSEIKSAYRKLARSYHPDVNKEPGAEQKFKDISNAYEVLSDDEKRSIYDRYGEAGLKGAGVGSGDFSNPFDLFESLFDGLGGMGGMGGMGGRNTRNRATQGEDQGYNLVLNFKEAIFGVEKEIEVTRLESCGTCNGSGAKPGTSPSKCTTCGGQGQVVSSARTPLGVFQQVMTCSSCNGSGEISTPCNTCNGDGRVRKSKRISLKVPAGVDAGSRLRVRSEGNAGRKGGPPGDLFVMIDVLQDPVLKRDDTNILYTCKISYIDAILGTTLKVPTVDGMVDLKIPPGTQPGTTLVMAKKGVPLLNKNNMRGDQLVRVQVEIPKRLSGEEKKLIEELSNLKKGKVPNSSGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C320</accession>
    <name>A0AAD5C320_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_031262</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009718">
        <property type="protein sequence ID" value="KAI7734463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C320"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7734463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="76" mass="8761" checksum="582948E1071D2892" modified="2024-05-29" version="1" fragment="single">MIKANPLSVLLMLRRKVIHKCTLHRDTLMSSPLILLLSMPLRRSRWNNNNEDLSAVLRLCVAVAKMLFGLPKRRPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C4S0</accession>
    <name>A0AAD5C4S0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_003003</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009653">
        <property type="protein sequence ID" value="KAI7734750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C4S0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045881">
        <property type="entry name" value="MNM1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34682">
        <property type="entry name" value="AT HOOK MOTIF-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34682:SF10">
        <property type="entry name" value="AT HOOK, DNA-BINDING MOTIF-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="202"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="273"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="278"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7734750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="312" mass="33643" checksum="3FB0650D4301AE94" modified="2024-05-29" version="1">MNQQNISKAPEQLVKRKRGRPRKDESAARNVKQHTQPPLAMAAPPAPAPQPAATIFPTSNNVAKVDPSMVGQVVTCVIDGIFDNGYLLSACVGPNNTVLRGIVFQQGHVAPITPANDVAPHVKMCTRGEFPILPSNPGNPIQISTPEPACAKQPVQPVAMQNYQNTPVMVTGGSSTSLQQESLTLIQQDNLMQVFEVSKPVEGPQDCNQRAKNEHVVSDPTADNFPETDTIVNKATELETVVNKQPAKEPEGEKPEVITPVEPIVENQPPVANEGLQHESQAQTGEISNIDANQHQNHEIGVDLSLSNFPEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C656</accession>
    <name>A0AAD5C656_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_010800</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009441">
        <property type="protein sequence ID" value="KAI7735735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C656"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46080:SF3">
        <property type="entry name" value="MITOCHONDRIAL SUBSTRATE CARRIER FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46080">
        <property type="entry name" value="MITOCHONDRIAL SUBSTRATE CARRIER FAMILY PROTEIN J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 6 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="26"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="313"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="6">
        <location>
            <begin position="24"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="6">
        <location>
            <begin position="122"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="6">
        <location>
            <begin position="310"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7735735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="443" mass="47127" checksum="4C675BEE2A0D8BCE" modified="2024-05-29" version="1">METARSGAGSFAQTEVNWDKLDKTKFYVVGAGIFSALTVGLYPISVVKTRLQVATKDSVEKNAYAVIRGLLKTEGIPGFYRGFGTVITGAIPVRIVLLSALETTKVAAYKMLEPFSISDPSKAAIANGIAGLTSSLCAQFVFVPVDVISQRLMVQGYSGHASYNGGLDVARKVLKQDGIRGLYRGFGLSVMTYSPSSAVWWASYGSSQRVIWNLLGHGSDGNGSIPSQGTVILVQGAGGIFAGAASSLITTPLDTIKTRLQVMKHENRPSTRQVVKALIAEDGWKDFMATARSETGYFAQTEINWDKLDKTKFYVVGAGIFTGLTVGLYPISVVKTRMQVTTKDSVEKSAYAVIRGLLKAEGVPGLYRGFGTVITGAVPARVIFMTALETTKVAAFKIVEPFTLSEPTKAAIANGIAGMMASLCSQGVFVPIDVNGSALKIHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C7K9</accession>
    <name>A0AAD5C7K9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_011236</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Secondary metabolite biosynthesis; terpenoid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009192">
        <property type="protein sequence ID" value="KAI7736801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016712">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051762">
        <property type="term" value="P:sesquiterpene biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11072">
        <property type="entry name" value="CYP71-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000011">
        <property type="entry name" value="Cytochrome P450 83B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47955">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 71 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47955:SF9">
        <property type="entry name" value="PREMNASPIRODIENE OXYGENASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 12" id="KW-0349">Heme</keyword>
    <keyword evidence="9 12" id="KW-0408">Iron</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1151"/>
            <end position="1170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1177"/>
            <end position="1196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1268"/>
            <end position="1293"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="12">
        <location>
            <position position="1038"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7736801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1710" mass="193855" checksum="4B3A47B12CD308F7" modified="2024-05-29" version="1">MDIFTIFPSWLLTAASVLFMLFLYTLRSHRSSMAVTNYKLPPNPPKLPIIGNLHKLYGKHRHEALWQLSKEYGPVMQIHIGSKPFLIISSPAMAKQVLKTHDHIFCSRPLSKARKLLTYNYMDIAFSAPSDHQKEMRRILVSELLGPKRARSVNHVLGMEIESMVSFLSSHPPNVAVNLSKLILEMVKGAVCKVAFVGGYDVFPGTVALVNAWGIGRDPSIWGKNAAEFYPERFENLEVDFEFIPFGSGRRSCPAKNIAPSTVEIVIANLLYWFDWEVPDGVKNKELNMQEEGSLLLHQIDHPYHRHEALWQLSKEYGPLMLFHIGSKPFLIISSPSMAKQATKQLTHNYLDIAFSPQSDHQREMRKILVSEFLESSIVNHLLVTEIETTVHGLELHPPNVAVNLNKLFLAIAKEIVCKVAFGKNYRQQPIKGPSWEVLVVEALEMLGGSVGDSFPWVGHFIDHVSGWNRKLATSFSNLDSYIESIVDDHKNQSSEVSDDEKDFVHGLLELSSKDDTSGHHLTKEDIKALIMHLGRECAEFYPERFKKFEVDFEMVPFGGGRRSCPAMNIAPATVEIVIANLLYLFDWEVPNGMKNEELNIKEAVLFLFRILQYTARFTGSSMTITKHPPGPQRLPIIGNLHQLVGKHRHQALWKLSQTYGPVMLLYFGSKPYIIISSSALAKQILKTNDLLFCTRPFYEGTKVLTYNYLDIAFSPYDNHWKEMRKVFVSELLGPERAGLFNDVTEMEIEGVVQSIYSHLGTVVNLENKLLALVFAVVCKVAFGKSYREEPFRGATLKEMLDEAKHMLGGSIGDHFPIIGRKLDKLSGWSDRLDKCFSNLDGYVQMVLDEHLDHGGSEISGHEKDFVHALIELSSKDSRGSGLSKDDIKALIMDVLTGGTDTIVVTMVWAMSEIVRNPRVMQKLQNEIRSCIGRKQKIHKADIMKMQYLKMVVKETLRLHSPAPLLIPRECTQHCQVGGYDVFPGTRILINAWAIGRDSRIWGDNSTEFYPERFENVEADFVGENFDMVPFGGGRRSCPAFNQAVTTVELTIANLLHVFDWEVPDGLRNEELNMEEKGSLVVCRKSSLCLVPRKYNWELKQVCNKPGQLLFKGSTHLMKAFICYIQLKETYSHSLIQNFVTNLSNFRSGSVFLFIELKSFFLLVIIIYCTRMILMKMYCNGGLGFVISIRIGFVIYCNITFQLAYVLTYKLLYVLTLLCLDVKVRSNPTKRTNPGSIPFLLIPGHTGPINTKQNFNIYTTLGSHLMEFFTIFPSWLLTTASVFFMLCMFLYTLRSNRSSITSPKLPPSPPKLPIIGHLHKLIGKTHHQALWQLSKQYGPIMLLHIGSKPFLIISSPAMAKQVLKTQDHIFCSRPLSKAAKRLTYDYLDIAFSPQSDHRKEKRKILVSEFLGPKRARSFNHVLVMEIESMVSVLSSQSSNTEVNISKMLLALVKEVVCKVGFGKNYSEPIEGSSWEEILEEAVVMLKGSLSDNFSLVGEIFDKISGWNSKLEKCFGNLDAYIQMIVDDHNNHKIAEISEDEKDFVHRLIEMSSMENASNHQLSKEDVKALIMNVFTGGIDTTVVAMEWAMSEIARSPRVMQKLQNEIRNCTGRIQKNAAEFYPERFEKLDLDSGVANFDMLPFGGGRRSCPAINTAPITVELVIANLLYWFDWELPESLKNKTLNMEEEGSLIIHKKVPLCLVPTKHTWED</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C932</accession>
    <name>A0AAD5C932_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_013249</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009110">
        <property type="protein sequence ID" value="KAI7737129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C932"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002403">
        <property type="entry name" value="Cyt_P450_E_grp-IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050665">
        <property type="entry name" value="Cytochrome_P450_Monooxygen"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24282">
        <property type="entry name" value="CYTOCHROME P450 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24282:SF234">
        <property type="entry name" value="CYTOCHROME P450-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00465">
        <property type="entry name" value="EP450IV"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11 12" id="KW-0349">Heme</keyword>
    <keyword evidence="8 11 12" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 12" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="7 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="binding site" description="axial binding residue" evidence="11">
        <location>
            <position position="454"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR602403-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7737129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="506" mass="57683" checksum="FE9DB2BDA50536F5" modified="2024-05-29" version="1">MEITTVFCALVVAAIFFFYAWRASNRLWFKPKRIEKFLKEQGLKGTPYKFMYGDFKEMVQSTNEAKSQPMSLTHDIAPRVSPFFHKSITTLGKHCFTWMGTKPLVHISDPTMIREILANHNKFQKPRGGNPLTKLLARGLLDAEGDQWVKHRKIINPAFHMEKLKNMVPAFYASCNEMIEKWEELVYTENSCEVDVWPHLQTFSSAVISRTAFGSNFKEGENIFELQREQAELIINATNSIYIPGSRFLPTKNNKRMKEIEQQVKGSIKGMINNRVVAMKANEASNDDLLGILLDSNYKEIKLHGNSNLGLSIDEVIEECKLFYFAGQDTAGSHHSEWQARAREEVLHVFGDKNPDIDGLNHLKVMNIIFNEVLRLYSPVVLLRRLIPEETKLGNLILPPGTLVQINTLLLHHDKDMWGEDVNDFNPRRFSEGVSKFTKGRAAYLPFGGGPRICIGQNFAMLEAKMALSMILQRFSFELSPSYSHAPHAVATMQPQFGAHLILHKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CD16</accession>
    <name>A0AAD5CD16_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Branched-chain-amino-acid aminotransferase-like protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_020066</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008696">
        <property type="protein sequence ID" value="KAI7738905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CD16"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046394">
        <property type="term" value="P:carboxylic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001594">
        <property type="entry name" value="Branched-chain-amino-acid aminotransferase-like protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.10.10:FF:000002">
        <property type="entry name" value="D-alanine aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.10.10">
        <property type="entry name" value="D-amino Acid Aminotransferase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001544">
        <property type="entry name" value="Aminotrans_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036038">
        <property type="entry name" value="Aminotransferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043132">
        <property type="entry name" value="BCAT-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043131">
        <property type="entry name" value="BCAT-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050571">
        <property type="entry name" value="Class-IV_PLP-Dep_Aminotrnsfr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42743">
        <property type="entry name" value="AMINO-ACID AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42743:SF11">
        <property type="entry name" value="AMINODEOXYCHORISMATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01063">
        <property type="entry name" value="Aminotran_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19798">
        <property type="entry name" value="Sulfotransfer_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56752">
        <property type="entry name" value="D-aminoacid aminotransferase-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7738905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="511" mass="57130" checksum="D22D28713A0090A7" modified="2024-05-29" version="1">MAEEIETEVIHSWSAPRSLSTSLMYSFAQRDDTEVLDEPLYAHYLKVTGVDRPYREELLSKMESDGNKVVNNVIYGPGGKKYRYCKHIAKQRLPGLSGELTKKGKHFILIRNPLDILQSFDKVVPSSFHELGLGDLVSVYSELCAAGNRPPIIDAAELQEDPEAALRGLCEDLDIPFQASMLKWEAGPKSVDGIWAPWWYKSVHKSTGFSRAINYPHAFPRSLYEVLEQSLPFYNMLRCHARQKRFPKLAKPSLPVPANEKILVWVDDELLPRDSATVSVFDSVVQGGDSVWEGLRIYNGKVFKLEEHLDRLFDSAKALAFNNVPTRDYVTSGMSPGFNLYGCTLIVLAEWKPPVYDNTGGITLVTATTRRNSPNIEGNNANADDAIMLDKDGYVSETNATNIFLVKKGIVMTPHADYCLPGITRATVMDLVKKENLVLEERRISLSEFHTADEVWTTGTMGELSPVLKIDGRVVGDGIVGPITKRLQNSFKKLTEGLGETRAVHHDRDQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CE77</accession>
    <name>A0AAD5CE77_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_020451</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008710">
        <property type="protein sequence ID" value="KAI7738861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CE77"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054502">
        <property type="entry name" value="bHLH-TF_ACT-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051358">
        <property type="entry name" value="TF_AMS/ICE1/BHLH6-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31945:SF15">
        <property type="entry name" value="TRANSCRIPTION FACTOR BHLH61-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31945">
        <property type="entry name" value="TRANSCRIPTION FACTOR SCREAM2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22754">
        <property type="entry name" value="bHLH-TF_ACT-like_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="6">
        <location>
            <begin position="178"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="217"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7738861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="354" mass="40346" checksum="6D8D6F12C30B9291" modified="2024-05-29" version="1">MEFHHHSHGFLEEILDSNNIASFGINDIDDQFDHNALLATPNTLLESSPLSMTSQTSSSFEDFSLPFGFDPQTLNCSSFGQHYINPFIDHDLLSAATIHHNSTPSNIKLESPPLYNDQDYYSMLSMLDDVQNCHFMHDVSNRVNPEMPYPQGVMGDLEPERPTSFNMGKNGKNCKVEGQPSKNLMAERRRRKRLNDRLSMLRSVVPKISKMDRTSILADTIDYMKELIQKIKHLQEDIEMEMEMDTSSSSNQFDLITAKPKEIFIRNSPKFDVERRNSDTRVKVCCNGKPELLISTMSTLEALGLQIHQCVISCFNDFTMHASCSEEMEQRLVLNTDDIKQALFRNAGYGGRCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CF41</accession>
    <name>A0AAD5CF41_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_024680</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008428">
        <property type="protein sequence ID" value="KAI7740437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CF41"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036488">
        <property type="entry name" value="DUF1883-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021910">
        <property type="entry name" value="NGX6/PGAP6/MYMK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14319">
        <property type="entry name" value="FIVE-SPAN TRANSMEMBRANE PROTEIN M83"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14319:SF3">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12036">
        <property type="entry name" value="DUF3522"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141099">
        <property type="entry name" value="Atu1913-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="686"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="746"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="771"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="9">
        <location>
            <begin position="525"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="529"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="556"/>
            <end position="565"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7740437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="828" mass="93071" checksum="D41E78434D0CE392" modified="2024-05-29" version="1">MEKKTNYSILGCLYSNQVYFFIFVGSFIIICCCSYEINAVSTESFTLSSFTYGRTVLKPYDWRYIRVDLPTWFSSMTISLESDVDLDKSRFRRADKGSLPMICAREGSPPLPDTHNTSFTGLVWDPISNLSLAIEGLQFSEKCYPMQKNILIKLTNEQISPGVWYFGLFNGIGSTRTQSKMINRGSGYSFSGNVSIEGCLSPSISGQFCNETVDHLSCVVQNSTQGIVTSCRNDGERSCIHQNESKLYSLDLLGVTEEITISATNVIFNQTQLSKGANNDSNIILMCYARHGSISSPSTHDYSGNINNAPLVIRSPKVGRWYITVTSLNTSKEITGSLDVCYSLEWKLLQCPINKAGLNCTWERYTLQTIMRKNPSVPFESYYLPDSDKVSSNSPNFPLEPLLSNSSLGQNQSQHFGWTYFLVDIPSGASGGSIHIRLNSDKKINHEIYASYGGLPLEDKWDYFYANSTSNSNGSMFFKLYDSDEKTISFYIVYVRGGTWSFGVKHLTSAGNALKSQGQTTMSISIERCPRRCSSHGTCQNIVEMSGLSLYSYCWCDRTHGGFDCSVEIVSHQGHIWQSVSLIASNAAFVFPAYWALRQKAFAEWVIYTSSGISSGLYHACDVGTWCALSFRVLQFMDFWLSFMAVVSTFVYLADIDEGSKRTIHTVVAILTALMAENGPTRSSNIVLVIAIGAAGLLVGFLIEFFRHYRRFSFSAELLLNMLHRWQTIKEWFHNLIKTIVKRFRWFFVVAGFAALAMAAISWNLESTESYWFWHSMWHVSIYISSFFFIVSKVNGASNGEAPNANYELTRQDSLSRVQDRPENGRVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CGI7</accession>
    <name>A0AAD5CGI7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">25.3 kDa vesicle transport protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_001133</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
            <topology evidence="2">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008250">
        <property type="protein sequence ID" value="KAI7741187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CGI7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.50">
        <property type="entry name" value="Longin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044565">
        <property type="entry name" value="Sec22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45837">
        <property type="entry name" value="VESICLE-TRAFFICKING PROTEIN SEC22B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0653">Protein transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="172"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00046280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7741187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="190" mass="22136" checksum="DA140A3ED25078A6" modified="2024-05-29" version="1">MVKLTIVGRVHDGLPISQGPIYDEDDIVYKKHAEFLLHEISMDALPPSTTTIFLHNHCFKKLAFHYLQDLQKEFDKVDPQLVNHISKPYSFVKFDNIICNIRRQYIDTRTQTNLSKLNADHELDQLDVLTEEMSVIVERRRRSDMLEKMKNAHKSTSPVWESKTLEVIAAKWTPITIIFVVAFVLLWSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CGT3</accession>
    <name>A0AAD5CGT3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MCM C-terminal AAA(+) ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_023834</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MCM family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008234">
        <property type="protein sequence ID" value="KAI7741224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CGT3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042555">
        <property type="term" value="C:MCM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017116">
        <property type="term" value="F:single-stranded DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006271">
        <property type="term" value="P:DNA strand elongation involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902975">
        <property type="term" value="P:mitotic DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630:SF66">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0235">DNA replication</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MCM C-terminal AAA(+) ATPase" evidence="5">
        <location>
            <begin position="65"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7741224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="107" mass="11493" checksum="D2637CB27A4C4C9C" modified="2024-05-29" version="1" fragment="single">VNPSSATCSDDNGGRPCWLDGDGVASVNWNISRGPIRIHPLKGVRAEKIIKATLFVLSQYHSLLKSQLLEYIHKLAPRGIYTSGRGTSAVGLTGYVAEDPETGETIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CJ43</accession>
    <name>A0AAD5CJ43_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_013431</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007902">
        <property type="protein sequence ID" value="KAI7742778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CJ43"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7742778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="46" mass="5474" checksum="AA940E2D41872D0E" modified="2024-05-29" version="1" fragment="single">MIPSLKRLQFEIHVKPEDTRFYALDFYSIRFRPSWICGAGIVEIVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CMT2</accession>
    <name>A0AAD5CMT2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_031548</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007620">
        <property type="protein sequence ID" value="KAI7743970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CMT2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7743970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="73" mass="8582" checksum="E9921D635DF627B2" modified="2024-05-29" version="1" fragment="single">QTTTFTKDNNRRFFFSFSFNQHLQFSSIHQSINQSIPIHTCFLEMASSSDPWNREYSEASKLADDITNMISER</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CNF9</accession>
    <name>A0AAD5CNF9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">mitogen-activated protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_032746</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.24" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010007548">
        <property type="protein sequence ID" value="KAI7744330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CNF9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004707">
        <property type="term" value="F:MAP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07859">
        <property type="entry name" value="STKc_TDY_MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000017">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000046">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003527">
        <property type="entry name" value="MAP_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01351">
        <property type="entry name" value="MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="17"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="508"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="524"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7744330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="565" mass="64302" checksum="21E7B022104D13C3" modified="2024-05-29" version="1" fragment="single">SPKEVEFFTEYGESNRYKIMEIIGKGSYGVVCAAVDTQTGEKVAIKKITNIFEHSSDAIRILREIKLLRLLRHPDIVEIKRIMMPPSRRDFKDIYVVFELMESDLHQVIKANDDLTHEHHRFFLYQMLRALKYMHSANVYHRDLKPKNILANANCKLKICDFGLARAAFTDTPTTIFWTDYVATRWYRAPELCGSFYSKYTPAVDIWGIGCIFAEVLTGKPLFPGKSVVHQLELITDLLGTPSSDTISKVRNDKARKYLTDMRKKKPVSFSEKFQEVDPLALTLLQRLLAFDPKDRPTAEQALADPYFKGLSKIEREPSCHSFSKLEFEFERRRVTKDDIRELIFREILEYHPQLLKDYMAGNDGSNFLYPSAIGQFRRQFAYFEENVGNSGPVIPPERKHVSVPRSTIASSTTAPKLQQCLAAFDNRPLVDSVSHGGGDVDASGNIFSAASRPPPRMPKARVVGPVLPLESSRSMKDMYDPRVMVRNTNTVGLSPHYAYRSNTGDQIHSQQKSTDGKLAPGLVNNNPYYKQTHGQQQSKERIAIDAKFMQPQSTQREVGVFNMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CQ43</accession>
    <name>A0AAD5CQ43_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_009983</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007166">
        <property type="protein sequence ID" value="KAI7745672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CQ43"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7745672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="90" mass="10609" checksum="B098341C7F2E5EE0" modified="2024-05-29" version="1" fragment="single">FFKSDIRELTFVLQKRFKFPVKQCRALCWKSQQQGAICYCIERIYGVIVGGEPKAQRVDRFWIKTLASYTSTLIGFFFGLFFISTKYTNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CQI5</accession>
    <name>A0AAD5CQI5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Hydroxyproline-rich glycoprotein family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_003212</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007397">
        <property type="protein sequence ID" value="KAI7744980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CQI5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040420">
        <property type="entry name" value="At1g76660-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31798">
        <property type="entry name" value="HYDROXYPROLINE-RICH GLYCOPROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31798:SF10">
        <property type="entry name" value="OS02G0822000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="155"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="213"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="223"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7744980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="440" mass="47331" checksum="8D01A3679D29E583" modified="2024-05-29" version="1">MNMGSINNTVETVNAAATAIVSAERRVQPTSVQKRRWGSCWSLYWCFGSYKQSKQIRHALLAPEPALLGPAPSVTQNVNSNTIIFPFIAPPSSPASFLQSDPPSATYSPVGLLSFKSLSQNPYPQGVPSSVFTVGPYAYETQLVSPPVFSTYTTEPSTASFTPPPESVQITTPSSPEVPFAQLLTSSLARARRHSMGSSQKFPLSQYEFQPYTGSPGGGVMSAGSTIPNSGTSSPFPDKQQRVLKLRVGDGSKFIGYEYFSTRKWGSRLGSESATPTGVSGSATPYGYGLGSRIGSGSLSPIGVGEMYHMESQISEVASLANSATSSPKREPLLDHRVSFELTHLDAASGTGVANNLEASFRTSSGNETLLNKTESVCECCVENKNKNSSEIIETTLKECRCKRQSVSRDFNFDNRNGVELGSNNNWTFFPTLQQSVKPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-10-08" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CST1</accession>
    <name>A0AAD5CST1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_016272</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007018">
        <property type="protein sequence ID" value="KAI7746194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30231">
        <property type="entry name" value="DNA POLYMERASE III SUBUNIT EPSILON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30231:SF4">
        <property type="entry name" value="PROTEIN NEN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7746194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="324" mass="35897" checksum="4A81AEE89BC6CD88" modified="2024-05-29" version="1">MASLARHFGLGQQSHRSLDDVRMNFEVVKLCXXXXFLESSQQDTDNNGASLNASTINSCNRKFTLEVTRPNISPFSATATHTVPANLPNQVETINTQTSLIHTGPSVDRMETETLQSNEAMVEESSTTTVSNAFSSHNEFIDPDLVSLASITVMVAPFVNGPHRIQILHRDIPMQIHCNGMRIRFELSTQYVDHAGNPRLSFVVDVGSPKLCNILDACDDIAKRVDSGGNTQWRPIVRKTGLNNTIRLNIPTAAEDTTRWXXXXYYKNSSSIPCTQQLVSSVTELGSLLREGSLVDAYFSLDPYNYQQNAGIRLLAKKLIIYTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CT86</accession>
    <name>A0AAD5CT86_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_015342</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006983">
        <property type="protein sequence ID" value="KAI7746434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CT86"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050872">
        <property type="entry name" value="PPR_P_subfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46128">
        <property type="entry name" value="MITOCHONDRIAL GROUP I INTRON SPLICING FACTOR CCM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46128:SF157">
        <property type="entry name" value="PENTACOTRIPEPTIDE-REPEAT REGION OF PRORP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="167"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="202"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="247"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="282"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="317"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="353"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="388"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7746434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="536" mass="62771" checksum="C376EA83915737E7" modified="2024-05-29" version="1">MGCFVCVHEHQEKQDFDDMGWFVSVQEKQEKQENQEKQKLDDMGCFLGVQEHQEEQENQEKQEKQKLDDMGCVLGVQENQEKQEKQKLDDMGCFLGVQKHQEKQENQEKQELDDMGRFVGVQEEQENQEKQEIEDFYFLFDTLCKYGYAKVATEVFNKWKGWRFKADAKMYTILIYGWCKVNKIKMAERFFNEMLESGIEPNVVTYNVVLNGMCRRASLHPEGRFERTVKGAEKVFDEMVRRGVEPDVTSYSILLHVYSRAHKPDGTLEILKTMKDRGVDPSLASYTSVVKCLCSCGRVEDAYKLLDEMVQNGVTPSATTYNCFFKEYRGRKDVDSALKLYKKMKRDSVFIPDTHTYNILLGMFMKLDRFDLVKEIWDDMKGTGSGPDLDSYTLLVHHLCEQQKWQEACGYFVEMIEKGILPQKATFETLYKGLIQADMLRTWRRLKKKLDDEFISSVLGDEWLVTDLQTVFWKSITPQACLMREAIEAVMWTSRMQLEAPPHEQILQHKSISNTGFWEVREPKWKRETAKVRVNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CUU9</accession>
    <name>A0AAD5CUU9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_027982</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006552">
        <property type="protein sequence ID" value="KAI7748334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CUU9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14707">
        <property type="entry name" value="bZIP_plant_BZIP46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000036">
        <property type="entry name" value="ABSCISIC ACID-INSENSITIVE 5-like protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043452">
        <property type="entry name" value="BZIP46-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22952:SF385">
        <property type="entry name" value="ABSCISIC ACID-INSENSITIVE 5-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22952">
        <property type="entry name" value="CAMP-RESPONSE ELEMENT BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BZIP" evidence="5">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="41"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7748334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="66" mass="7711" checksum="5EF75D25E0BE09F5" modified="2024-05-29" version="1">MIKNRESAARSRARKQAYTHELENKISRLEEENERLKRQKEVGMVLPSAPPPNPKYQLRRTSSASF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CVB7</accession>
    <name>A0AAD5CVB7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_012656</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006860">
        <property type="protein sequence ID" value="KAI7747005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CVB7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7747005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="113" mass="12289" checksum="52E94213DD467FE4" modified="2024-05-29" version="1">MDLEAVADGPVNASSTTGIPTEGDRDSEQNENVKMDCGLVDSSESVSSMRVEKRKAAAAAAEEAKKRDLNVMHEDTLEAAMLDLEELVNKVKWLQRILQSSATSTSSSWKFAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CW08</accession>
    <name>A0AAD5CW08_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_004272</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006389">
        <property type="protein sequence ID" value="KAI7749281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CW08"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7749281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="164" mass="18534" checksum="2FBC55C9D2CE4258" modified="2024-05-29" version="1">MIADHMGSNVPRANSAFVKEESYEATAWMIKMIGSTMDQQMKRPKTKIFCTLGPGSRSVRIRCVEPAVKSLRGFDGVEEIETNTKDDRVTVKGKSIDAVRIELMEQDLFELQQLLSGKKEQEKAMLDVQTYVLLKVEQDQKITEDAHISAERDAAAQEYLEHSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CWC0</accession>
    <name>A0AAD5CWC0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Aquaporin TIP2-1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_024791</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Channel protein in tonoplast. These proteins may allow the diffusion of amino acids and/or peptides from the vacuolar compartment to the cytoplasm.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the MIP/aquaporin (TC 1.A.8) family. TIP (TC 1.A.8.10) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006495">
        <property type="protein sequence ID" value="KAI7748657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CWC0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015250">
        <property type="term" value="F:water channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019755">
        <property type="term" value="P:one-carbon compound transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00333">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1080.10:FF:000002">
        <property type="entry name" value="Probable aquaporin TIP1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="entry name" value="Glycerol uptake facilitator protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034294">
        <property type="entry name" value="Aquaporin_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000425">
        <property type="entry name" value="MIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022357">
        <property type="entry name" value="MIP_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00861">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45665">
        <property type="entry name" value="AQUAPORIN-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45665:SF9">
        <property type="entry name" value="AQUAPORIN-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00230">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00783">
        <property type="entry name" value="MINTRINSICP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81338">
        <property type="entry name" value="Aquaporin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00221">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="55"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="85"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="140"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="172"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="215"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00053065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7748657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="248" mass="25122" checksum="040BDB454C7E3FED" modified="2024-05-29" version="1">MPGIAFGRFDDSFSLSSIKAYIAEFISTLLFVFAGVGSAIAYAKLTSDAALDPAGLVAVAVCHGLALFVAVSIAANISGGHVNPAVTFGLAVGGQITILTGIFYWIAQLIGSIVACYLLNFVTGGLAVPTHGVAAGVGAIQGVVMEIIITFALVYTVYATACDPKKSALGTIAPLAIGLIVGANILAAGPFSGGSMNPARSFGPAVASGDFSGHWIYWVGPLIGGGLAGVIYPNVFMNHEHAPLSSEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CWH4</accession>
    <name>A0AAD5CWH4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">PHD finger protein ALFIN-LIKE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_011332</name>
        <name evidence="15" type="ORF">M8C21_016125</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">AA19_3_7</strain>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Histone-binding component that specifically recognizes H3 tails trimethylated on 'Lys-4' (H3K4me3), which mark transcription start sites of virtually all active genes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Interacts with H3K4me3 and to a lesser extent with H3K4me2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">The PHD-type zinc finger mediates the binding to H3K4me3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the Alfin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006715">
        <property type="protein sequence ID" value="KAI7747633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAMZMK010006714">
        <property type="protein sequence ID" value="KAI7747635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CWH4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd15613">
        <property type="entry name" value="PHD_AL_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000306">
        <property type="entry name" value="PHD finger alfin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045104">
        <property type="entry name" value="Alfin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021998">
        <property type="entry name" value="Alfin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044104">
        <property type="entry name" value="PHD_AL_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12321">
        <property type="entry name" value="CPG BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12321:SF178">
        <property type="entry name" value="PHD FINGER PROTEIN ALFIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12165">
        <property type="entry name" value="Alfin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="3 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0804">Transcription</keyword>
    <keyword evidence="7 11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="13">
        <location>
            <begin position="185"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="138"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU369089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7747633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAI7747635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="241" mass="27361" checksum="A26B2AA948EACD51" modified="2024-05-29" version="1">MSTISSSPRTVEEIFKDYSARRAGIVRALTYDVDEFYATCDPEKENLCLYGHPNETWEVTLPAEEVPPELPEPALGINFARDGMHKRDWLSLVAVHTDSWLLAVAFYFGARLNRNERKRLFSMINEVPTVFEVVTERKHVKDKPNADNGNKSRSSTKRTNDGQSKSTPRVYDDSFAEDEEDEHGETLCGTCGGNYSGDEFWIGCDICERWYHGKCVKITPAKAESIKQYKCPSCSTKKSRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CWX1</accession>
    <name>A0AAD5CWX1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Elongation factor Tu</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_008458</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 7">This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006274">
        <property type="protein sequence ID" value="KAI7749806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CWX1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070125">
        <property type="term" value="P:mitochondrial translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01884">
        <property type="entry name" value="EF_Tu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03697">
        <property type="entry name" value="EFTU_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03707">
        <property type="entry name" value="EFTU_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000001">
        <property type="entry name" value="Elongation factor Tu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000003">
        <property type="entry name" value="Elongation factor Tu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00118_B">
        <property type="entry name" value="EF_Tu_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041709">
        <property type="entry name" value="EF-Tu_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050055">
        <property type="entry name" value="EF-Tu_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033720">
        <property type="entry name" value="EFTU_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004541">
        <property type="entry name" value="Transl_elong_EFTu/EF1A_bac/org"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004160">
        <property type="entry name" value="Transl_elong_EFTu/EF1A_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00485">
        <property type="entry name" value="EF-Tu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000766">
        <property type="entry name" value="PRK00049.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009372">
        <property type="entry name" value="PRK12735.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009373">
        <property type="entry name" value="PRK12736.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43721:SF22">
        <property type="entry name" value="ELONGATION FACTOR TU, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43721">
        <property type="entry name" value="ELONGATION FACTOR TU-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03143">
        <property type="entry name" value="GTP_EFTU_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="6 7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="8">
        <location>
            <begin position="63"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7749806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="450" mass="49190" checksum="F0F1772216E186F4" modified="2024-05-29" version="1">MASVAFRNPNSRRLLFHHLRSTCRASVLSSSGFPTVESSSGNDTSSLAPNPWFRSMATFTRTKPHVNVGTIGHVDHGKTTLTAAITKVLADEGKAKAIAFDEIDKAPEEKKRGITIATAHVEYETAKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSAPDGPMPQTKEHILLARQVGVPSLVCFLNKVDAVDDPELLELVELELRELLSFYKFPGDEIPIVRGSALSALQGTNDEIGKNAILKLMEAVDDYIPTPERQKEKPFLMPIEDVFSIAGRGTVATGRVEQGTIKVGEEVEIMGLMQGSKKTTVTGVEMFKKSLDHGEAGDNVGLLLRGISRTDIQRGQVIAKPSSVKTYTKFEAEIYVLTKDEGGRHTAFFSNYRPQFYLRTADVTGKVELPEGVKMVMPGDNVAATFELITPVVLEPGAFPRNFFNFISHMHTALRSIPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CXR4</accession>
    <name>A0AAD5CXR4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_006639</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the autoinducer-2 exporter (AI-2E) (TC 2.A.86) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006553">
        <property type="protein sequence ID" value="KAI7748325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CXR4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002549">
        <property type="entry name" value="AI-2E-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21716">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21716:SF74">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN TQSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="253"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="318"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="348"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="384"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="414"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7748325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="435" mass="48008" checksum="0B00BF69C0809986" modified="2024-05-29" version="1">MAYEQFGVIGAIGLLGLGFVFSSNHVKSSLSVGLSFGRNSKQRVQSVHTAFLTKGVLNRLETFVAFGLIFGMIVGSVMGIMFFSYKIGVEGKDVVYSIKSHVEENNYAEKIGLRKWMDENDVPGMVDRYTTQFYETVNEQIDSLGVQYNVTEIVQEMKQLIVPRGLNVTNSSVSSTALATPNPYTEKILSLKRIVSNREWSAIYPEVNAMLNEARISKADVMEKAKAVAFQGKDIIQRVLASSKSIVGGSTKLVFIVIESVVSGAAGFLNFISQSMVFIWVLYSLITSDSGGVTEQVMYMIPIPRSTRKRCVEVLDRAISGVLLATAEIAFFQGCLTWLLFRLFNIHFLYTSTLLAFINPLLPIFPYFFSTIPAALQLVLEGRYIVAIFLSIIHLVLIDYGTTEIQEDIPGHSAYLTGLSIIGGVALFPSALEVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CYG8</accession>
    <name>A0AAD5CYG8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_027154</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006281">
        <property type="protein sequence ID" value="KAI7749760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CYG8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBC core" evidence="1">
        <location>
            <begin position="6"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7749760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="106" mass="11689" checksum="48BA086A1B30D7A1" modified="2024-05-29" version="1" fragment="single">VVDLVMASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVAFRTKVLLSICSLLTDPNPDDPLVPEIAHMYKTDKSKYEATARSWTQKYAMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D2B9</accession>
    <name>A0AAD5D2B9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_027103</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Secondary metabolite biosynthesis; terpenoid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005821">
        <property type="protein sequence ID" value="KAI7751770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D2B9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016712">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051762">
        <property type="term" value="P:sesquiterpene biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11072">
        <property type="entry name" value="CYP71-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000011">
        <property type="entry name" value="Cytochrome P450 83B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47955:SF16">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47955">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 71 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8 9" id="KW-0349">Heme</keyword>
    <keyword evidence="6 8 9" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="8">
        <location>
            <position position="444"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7751770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="502" mass="56719" checksum="DB86B55101706893" modified="2024-05-29" version="1">MELFSSLQTLSFPLLSCIILVIFICVRWISSNSYNRKNIPPSPRKLPIIGNFHQLGSSPNRNFQILSQKYGPLMLLHFGSIPTLVASSSEAAKEIMKTHDVSFCSRPSLIVPNIVFYGSKSISFAPYGEHWRQLKSLVVLKLLSNTRVKSYQKVRVDEIDHMIKVLGESCGTTIDMGLMFASLTNNIICRIALGRKLDEPKYKNLVSSLVDAFTVLNVGSYIPWLSWLDLVIGSVRKAEKIAKEFDEFLERIIEEHVNKKMREGSKTNGGEDFVDILLDLQEDNTSGFTLQRDSLKALILETFAAATETTQTGLEWTLSELIKNPRVMNKLQQEVTEIAQGRAMISEEDLEKMPYLKAVIKEALRLHPPLPLLLPRISVKDVKIMGYDIEAGTQVIINAWAIGRDPALWEESNKFRPERFLNNSITYQATQFEWLPFGAGRRLCPGIQFSVPVMELALANIVYKFDMTLPNGTKNEDLDMSETFGTAMHKKSPLLVLVTPRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D2R3</accession>
    <name>A0AAD5D2R3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_003042</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006077">
        <property type="protein sequence ID" value="KAI7750845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D2R3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000001">
        <property type="entry name" value="Calmodulin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039647">
        <property type="entry name" value="EF_hand_pair_protein_CML-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10891">
        <property type="entry name" value="EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="33"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="69"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="106"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="142"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="14"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7750845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="190" mass="21154" checksum="D3E974BEBC6FE696" modified="2024-05-29" version="1">MGLKNLFNRKKKPITTTTQNDAVSPSVNNSHHQIETELTQVFKKFDVNGDGKISSTELGSIMGSLGHRPTDEELEMMISNVDSDGDGFIDLNEFIELNTKGVGSTEVLESLKEAFSVFDIDKNGLISAEELQKVLGRLEEECTIGECRKMIAGVDVDGDGMISFEEFRGMMMRVDGFGFDKERMERESQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D3B9</accession>
    <name>A0AAD5D3B9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_022065</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ARG7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005786">
        <property type="protein sequence ID" value="KAI7751969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D3B9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009733">
        <property type="term" value="P:response to auxin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003676">
        <property type="entry name" value="SAUR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31929">
        <property type="entry name" value="SAUR-LIKE AUXIN-RESPONSIVE PROTEIN FAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02519">
        <property type="entry name" value="Auxin_inducible"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7751969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="100" mass="11255" checksum="8E1D8DC2D0EE9A7C" modified="2024-05-29" version="1">MAIRMPHISQARKILKRSLSNSSRTPATMDIPKGYFAIYVGEQEKKRFVVSLLLLSEPTFQQLLQQAEEEFGYNHPMGGLTIPCSEDAFMELASPFGAFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D5S5</accession>
    <name>A0AAD5D5S5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_017931</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005253">
        <property type="protein sequence ID" value="KAI7753857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D5S5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030865">
        <property type="term" value="P:cortical cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035303">
        <property type="term" value="P:regulation of dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21505">
        <property type="entry name" value="PPP2R3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000129">
        <property type="entry name" value="Serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039865">
        <property type="entry name" value="PPP2R3C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12085:SF6">
        <property type="entry name" value="CALCIUM BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12085">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE 2A REGULATORY SUBUNIT B'' SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="109"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7753857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="296" mass="33967" checksum="BBD5C9537D787475" modified="2024-05-29" version="1" fragment="single">VSLTQARIDMSELDEDSDGFLQPYEMEAYIRGLIPNLAQLRDMPAEFIQMYCHIATHKFFFFCDPSRRGKACIKKILLSNCLQELMELHQESEGEAADTEQPDNWFSLASTQRICDMFIDLDRDANGTLSEEELQGYADATLTDIFIQRAFDEHVRHGKTVNGLAWEMDLESFLDFVLALENKDTPEGLTYIFKCLDLQGKGYLTAADIHILFRDVREKWIQVGNYEVCIEDVRDEIWDMVKPVDPLRITLCDMLQCKQGGTIASMLIDVRGFWAHDNRENLLQEEGESLDIDGAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D6Q2</accession>
    <name>A0AAD5D6Q2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_016806</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005560">
        <property type="protein sequence ID" value="KAI7753051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D6Q2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46519">
        <property type="entry name" value="RING/U-BOX SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46519:SF11">
        <property type="entry name" value="ZINC FINGER, RING_FYVE_PHD-TYPE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="144"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="209"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="250"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="579"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="144"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="170"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="258"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="596"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="612"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7753051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="641" mass="73961" checksum="050B6D66EACDDDD7" modified="2024-05-29" version="1" fragment="single">MMTDVQQQILQKPESTTDACLNFERRGSDFERGFEEVMRGQLDECMSFASCNADDDDVSESGDQLIRRQAQEMISTIERRNRESELMALAGMQTVSMLDSSFLRESQSPSTNLRRQGNVERSSTRASTILQMWRELEEEHVLDRSRERVGVRLSPERSLDSSEHQGSLEDVTESENDYDQDNDGSSREQTPDIGEVEQERVRHIVQGWRETGVTDPAPDVAQRSGSRREEWLGETERERVRNVREWVQMTSQQRGTRGGREQSRERAGGQHDEGQTEHVRRDMARLRGRQALVDLLRKAERERRRELQDLTGYRAVSDFAHRNRIQSLLRGRFLRNERPVEEERPSSVAASELVQLRQRHTVSGLREGFRSRSENIVLGQESEHDNQDQSQLWGQETDIHQVTDHIGSLDTVASIQNANQQENINEDQREDWQQESSSYSEFNIRRGGNLEDAGMNWQENSGNDWRQETLETGGDGAHMHGTDEVWREDASQEAIENWSNGASDPPRMLHLGAVGRVNRFHLPDDDSVYSMELRELLSRRSVSSLLRSDFRESLDQLIQSYINRQDRASIDWDLHRPSVSLEGEGDQEHHQDEQTEQSQQDAIGGRPSLVLPSPPVPPPQPLWHHFPYSSWSRHTMHRSEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D758</accession>
    <name>A0AAD5D758_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_003717</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010003020">
        <property type="protein sequence ID" value="KAI7754589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D758"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009873">
        <property type="term" value="P:ethylene-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00018">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.730.10:FF:000001">
        <property type="entry name" value="Ethylene-responsive transcription factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.730.10">
        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001471">
        <property type="entry name" value="AP2/ERF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31677:SF229">
        <property type="entry name" value="(WILD MALAYSIAN BANANA) HYPOTHETICAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31677">
        <property type="entry name" value="AP2 DOMAIN CLASS TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00847">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00367">
        <property type="entry name" value="ETHRSPELEMNT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00380">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51032">
        <property type="entry name" value="AP2_ERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0936">Ethylene signaling pathway</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0611">Plant defense</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="9">
        <location>
            <begin position="27"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7754589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="147" mass="16966" checksum="56CF3C8E00A67B1F" modified="2024-05-29" version="1">MTTNNLHHHNNHPNTTITTAPPQKELRYRGVRKRPWGRYVAEIRDPWKKTRRWLGTFATAEEAALAYDAAAFTLRGAKAKMNFGFTPYLRPVVEKKDNFGGDLILNLGYEKQSVDYVKVNDDHDDDVKVETMKKPFLFDLNLPATLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D7U6</accession>
    <name>A0AAD5D7U6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_032654</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005294">
        <property type="protein sequence ID" value="KAI7753726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D7U6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7753726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="72" mass="7862" checksum="45DA99B791B95508" modified="2024-05-29" version="1">MPSWKAATDDDDDVGSLRDNRINISLGCHYISFGEPASGDTIRFSITTSTAESVTNVSINLIIIEGYSDEFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D894</accession>
    <name>A0AAD5D894_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_020840</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010002408">
        <property type="protein sequence ID" value="KAI7754837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D894"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052308">
        <property type="entry name" value="PPR_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47937:SF6">
        <property type="entry name" value="PENTACOTRIPEPTIDE-REPEAT REGION OF PRORP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47937">
        <property type="entry name" value="PLASTID TRANSCRIPTIONALLY ACTIVE CHROMOSOME 2-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="172"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7754837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="345" mass="38847" checksum="8218210D669129E8" modified="2024-05-29" version="1">MAVSVAKLCKPIHMFSPLFIRFSSPVHTISSQQLLEQSIKSAVEAKSYRQIADILNASKETSQAANPFSFLSSFHQEDRTKIIDEILQSFIPLRPRNHAQRAYTYLLSFTLQGPDPLPLSLAILQRTLRSGCTPVPQTHLLLSSVWIHQRKKFGHTVASMLLQMHSIGYKPDGGVCNYLISSLCKVDQYEEAVQVLRSMGGAGCVPDLDSFGSVIGLLCHLRKTKNIEELMKEMVSKFRLSPRKELVVTVLKSMRANKDAHKAVEMIIFLEEMDIQIGFESYELVVETCLESNLFVLAGKVVMRMTNKGFIPYIKVRQKVFDGLAAVGELEFAYILKKKFTELNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D9Q1</accession>
    <name>A0AAD5D9Q1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">65-kDa microtubule-associated protein 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_014502</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MAP65/ASE1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000360">
        <property type="protein sequence ID" value="KAI7756513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D9Q1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1520:FF:000002">
        <property type="entry name" value="65-kDa microtubule-associated protein 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1520">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007145">
        <property type="entry name" value="MAP65_Ase1_PRC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19321:SF0">
        <property type="entry name" value="65-KDA MICROTUBULE-ASSOCIATED PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19321">
        <property type="entry name" value="PROTEIN REGULATOR OF CYTOKINESIS 1 PRC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03999">
        <property type="entry name" value="MAP65_ASE1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="352"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="379"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="401"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7756513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="449" mass="51035" checksum="8CFD49D4BBDBB663" modified="2024-05-29" version="1">MMAVSPSSVRTATTCNTLLRELQQIWMDIGENEAEKDRMLMELERECLEVYRRKVDEAATAKARLHQAVAAKEAELATLIAALGDLNTHFPINIENKSSSLKAQLAMVTPLVEDLKLKDIAASLFELWQIMDSTREEKSYFSKITSVIRLSEAEIVEPAALSLEVIEQASQEVERLSKLKASRMKELVMKRRSELEAICLKTHIEPDPSTAIDKSNAMIDSAQISKVKDEALSRKEIMDRIERWLSACEEERWLEDYNLDQNRYSAGRGAHLSLKRAERARIAVNKIPTMVDNLISKTIAWENEKNKSFLYDGVRLVTILEEYKLTRLQKEEEKKRFRDQKKLQDLLLTERESIYGSKPSPRRASSFRMSNGHRGNGNGSMTPTPRRNSVASPTMEPMTPRSYSGRQNGYSKEMRRLTTAPLNFVAIPKEDTISFSSVYGSEPGSPLQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DDU4</accession>
    <name>A0AAD5DDU4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Leucine-rich repeat-containing N-terminal plant-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_018703</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000036">
        <property type="protein sequence ID" value="KAI7757982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DDU4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7757982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="101" mass="10562" checksum="429C5DCFC5202B18" modified="2024-05-29" version="1" fragment="single">MMRDSKLKIYAVAVVGLLSISTIPNCFGYTDPSDVVAINSLYVALGMPPLLGWLFAGGDPCLEGWQGVQCVNSNITGIVLNNASLGGELSENLGAFASIIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DE22</accession>
    <name>A0AAD5DE22_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">LOB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_017808</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LOB domain-containing protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000591">
        <property type="protein sequence ID" value="KAI7756095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DE22"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004883">
        <property type="entry name" value="LOB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31304">
        <property type="entry name" value="LOB DOMAIN-CONTAINING PROTEIN 38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31304:SF43">
        <property type="entry name" value="LOB DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03195">
        <property type="entry name" value="LOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50891">
        <property type="entry name" value="LOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LOB" evidence="2">
        <location>
            <begin position="46"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7756095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="223" mass="24466" checksum="8D22C6B2181E3EC4" modified="2024-05-29" version="1" fragment="single">KIRLKSTSFLHYKTTHTHTLVSRLPFYFLSKNPHKQTNQKTKQTNMSCHGCRVLRKRCGENCVLRPCLDWIESPEAKARATLFVSKFFGRGDLFSFISSVPVPKRNALFQSLLFEAVGRTVNPVNGAIGLLSAGNWHLCQAGVQTVLAGGTPTILSTQIPVTNGSSSVSQAIGGLSAMMIGNPAEDDEQCFSMMPESDVSNVPMSFRCSDGEDQEPKLLNLLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DEI5</accession>
    <name>A0AAD5DEI5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Methyltransferase</fullName>
            <ecNumber evidence="11">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_016936</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Endomembrane system</location>
            <topology evidence="10">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 11">Membrane</location>
            <topology evidence="1 11">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the methyltransferase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="11"/>
    <dbReference type="EMBL" id="JAMZMK010000013">
        <property type="protein sequence ID" value="KAI7758249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DEI5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000119">
        <property type="entry name" value="probable pectin methyltransferase QUA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004159">
        <property type="entry name" value="Put_SAM_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10108:SF1177">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10108">
        <property type="entry name" value="SAM-DEPENDENT METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03141">
        <property type="entry name" value="Methyltransf_29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 11" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="62"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU366043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7758249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="670" mass="75928" checksum="7A07D1103587CAB3" modified="2024-05-29" version="1">MSRPLHRGFSGGGRLSGHIQDFLEDSQMKIKPEKEDFDKNGYVVPDPFSSVSSRKRYKLTLFLLKLSLFVIVFLALTGSFWWTISLTTASRNHTFRGYRRLQEQLVSDLWDIGELSLGTSRTKETEFCSLESENHIPCFNISENLELGLTEGQEQDRHCRPTSKQNCLVLAPSKYKVPHRWPTGRDVIWIDNVKINAQEVLSSGSLTKRMMLLDDDQISFSFASSMVDDSIEDYSHQIAEMIGLRNESYLVQAGVRTVLDIGCGFGSLGAHFFQNHLLTMCIANYESSGSQVEITLERGLPAIVASFTTKKLPFPSLSFDMVHSAWDGIAWNHKDGIHLIEVDRVLRPGGYFVWTSSAANTRASVRKKDSSNIGDFVRNFAKDLCWNLLSQQDKTVVWKKTSSKDCYASRNHGSSPSVCKVGREVESPYYHPLEACIGGTHNRRWIPIEKRPKWPSRATLSSKELAAHGVLFDDVIEDDLNWKSAVRNYWSLLSPLIFSDHPKRPGIEDPIPPYNMVRNVLDMNAHFGGFNSALLGAGKSVWVMNVVPTSGPNHLPLILDRGFLGVLHDWCEAFPTYPRTYDLVHAEGFLSLETAKRRSCSMLDVFFEMDRILRPEGWVILRDTSSLIETARTITTRLKWEARVVEIESNSDEKLLVCQKPLIRRRSNPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G1E8</accession>
    <name>A0AAD5G1E8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Thaumatin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_017280</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012222">
        <property type="protein sequence ID" value="KAI7724437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G1E8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.110.10:FF:000013">
        <property type="entry name" value="Pathogenesis-related thaumatin superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.110.10">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037176">
        <property type="entry name" value="Osmotin/thaumatin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001938">
        <property type="entry name" value="Thaumatin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31048">
        <property type="entry name" value="OS03G0233200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00314">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002703">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00205">
        <property type="entry name" value="THN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49870">
        <property type="entry name" value="Osmotin, thaumatin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51367">
        <property type="entry name" value="THAUMATIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="13"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="18"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="26"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="47"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="57"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR002703-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7724437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="113" mass="12197" checksum="2358C2E0F23006FC" modified="2024-05-29" version="1">MLVVPRGGKGGACSSTGCLVDLNRACPTALRVARSGSRSVVACKSACEAFGDPRFCCSEGYNTPDTCPPTEFSAYFKHVCPRSYSYAYDDKTSTFTCVGADYMIIFCPLPYTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G1R7</accession>
    <name>A0AAD5G1R7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">M8C21_001674</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">AA19_3_7</strain>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="10">eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the DEAD box helicase family. eIF4A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="JAMZMK010011887">
        <property type="protein sequence ID" value="KAI7725649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G1R7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17939">
        <property type="entry name" value="DEADc_EIF4A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000089">
        <property type="entry name" value="Eukaryotic initiation factor 4A-II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000031">
        <property type="entry name" value="Eukaryotic initiation factor 4A-III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 13" id="KW-0347">Helicase</keyword>
    <keyword evidence="4 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="16">
        <location>
            <begin position="40"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="14">
        <location>
            <begin position="71"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="15">
        <location>
            <begin position="252"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="16">
        <location>
            <begin position="342"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="14">
        <location>
            <begin position="373"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="15">
        <location>
            <begin position="554"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="12">
        <location>
            <begin position="40"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="12">
        <location>
            <begin position="342"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAI7725649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="715" mass="81091" checksum="F276C7FBB1513007" modified="2024-05-29" version="1">MAGAAPEGSQFDARQFDSKMNELLSSDGQDFFTSYDEVYDSFDAMGLQENLLRGIYAYGFEKPSAIQQRGIVPFTKGLDVIQQAQSGTGKTATFCSGILQQLDYGVVECQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILSAGVHVVVGTPGRVFDMLRRQSLRADYIKMFVLDEADEMLSRGFKDQIYDIFQLLPSKVQVGVFSATMPPEALEITRKFMNKPVRILVKRDELTLEGIKQFYVNVEKEEWKLETLCDLYETLAITQSVIFVNTRRKVDWLTDKMRSRDHTVSATHGDMDQNTRDIIMREFRSADAQDFFTSYDEVYDSFDAMDLQENLLRGIYAYGFEKPSAIQQRGIVPFTKGLDVIQQAQSGTGKTATFCSGILQQLDYNIIECQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILSAGVHVVVGTPGRVFDMLRRQSLRPDYINMFVLDEADEMLSRGFKDQIYDIFQLLPTKVQVGVFSATMPPEALEITRKFMNKPVRILVKRDELTLEGIKQFYVNVEKEDWKLETLCDLYETLAITQSVIFVNTRRKVDWLTDKMRSRDHTVSATHGDMDQNTRDIIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTQPENYLHRIGRSGRFGRKGVAINFVTGDDQRMLYDIQKFYNVAVEELPSNVADLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G1V1</accession>
    <name>A0AAD5G1V1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Inositol oxygenase</fullName>
            <ecNumber evidence="4 11">1.13.99.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Myo-inositol oxygenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_011081</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>myo-inositol + O2 = D-glucuronate + H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23696"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:17268"/>
            <dbReference type="ChEBI" id="CHEBI:58720"/>
            <dbReference type="EC" id="1.13.99.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10 11">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="10 11">Binds 2 iron ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Polyol metabolism; myo-inositol degradation into D-glucuronate; D-glucuronate from myo-inositol: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the myo-inositol oxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.13.99.1" evidence="4 11"/>
    <dbReference type="EMBL" id="JAMZMK010011866">
        <property type="protein sequence ID" value="KAI7725754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G1V1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050113">
        <property type="term" value="F:inositol oxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019310">
        <property type="term" value="P:inositol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019853">
        <property type="term" value="P:L-ascorbic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007828">
        <property type="entry name" value="Inositol_oxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12588:SF12">
        <property type="entry name" value="INOSITOL OXYGENASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12588">
        <property type="entry name" value="MYOINOSITOL OXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05153">
        <property type="entry name" value="MIOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0060">Ascorbate biosynthesis</keyword>
    <keyword evidence="5 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 10 11" id="KW-0408">Iron</keyword>
    <keyword evidence="7 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="10">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR607828-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7725754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="279" mass="32678" checksum="4D0BD1F3FC557FD0" modified="2024-05-29" version="1">MTVSVEHSVPESQVVENKNHCYNTDELVLDGGFVVPDPVASGGFDAPEINSFGNTFRDYDVESLRNKQVEEFYRMNHINQTYEFVKKMREEYKKLDKLEMSIWECCELLNEVVDDNLGKVLLLPRFGELPQWAVVGDTHPLGCAFDESIVHHKYFKENPDINNPTYQTKNGVYEEGCGLENVMISWGHDDYMYLVAKENGTTLPQAGLFIIRYHSFYPLHRSGAYKHLMNEEDVENLKWLQIFNKYDLYSKSKVRVDVEKVKPYYLSLIKKYFPEKLRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G314</accession>
    <name>A0AAD5G314_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CRAL-TRIO domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_010782</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011533">
        <property type="protein sequence ID" value="KAI7726790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G314"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008526">
        <property type="term" value="F:phosphatidylinositol transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.525.10:FF:000008">
        <property type="entry name" value="Phosphatidylinositol transfer protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011074">
        <property type="entry name" value="CRAL/TRIO_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052578">
        <property type="entry name" value="PI_Transfer_CRAL-TRIO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45824:SF28">
        <property type="entry name" value="CRAL-TRIO LIPID BINDING DOMAIN, CRAL_TRIO DOMAIN, CRAL_TRIO DOMAIN SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45824">
        <property type="entry name" value="GH16843P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03765">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00180">
        <property type="entry name" value="CRETINALDHBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM01100">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46938">
        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="2">
        <location>
            <begin position="85"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="10"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7726790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="261" mass="30581" checksum="296CF841013771DD" modified="2024-05-29" version="1" fragment="single">MSIFRRRGSSHQQQEQDQPELQSESKVNELKTALGPLTGRSLLYCTDACLKRYLEARNWNVEKAKKMLEETLAWRSTYKPEEIRWHEVAVEGETGKLFRANFRDRFGRTVLVLKPGLQNTTSMDNQIKHLVYLMENAMLNLPEGQEEMAWLIDFTGWSFSTSVPVRTAKDTINILQDHYPQRLAVAFLYAPPRIFEAFWKIVKFFMDPKTSQKVKFVYPKNKESVELMRTYFDVDNLPTEFGGKATMKYDHEDFSRLMAQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G3D1</accession>
    <name>A0AAD5G3D1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_027601</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011403">
        <property type="protein sequence ID" value="KAI7727329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G3D1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7727329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="204" mass="23071" checksum="E8D846B53D4CDF35" modified="2024-05-29" version="1">MELKTDWVVQGCWDWARQPSNVAEMVEFTKYNAAIQRFNLTGSEYACHWTTASSDRIRKCQEAFIREWKMWPGLVKTAKEGVDAAEWSICCCRMEFWGPTRADVAKRRGRKLHCCSNKLRYFDLDSHANTLFVETKGGMIVVALAFAGHREGSNDVNVSITSSNDEYRYAGELSESSSSRTTNVLQALLLHIVAVTLGSSLWVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G4L1</accession>
    <name>A0AAD5G4L1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_016870</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011390">
        <property type="protein sequence ID" value="KAI7727376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G4L1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01837">
        <property type="entry name" value="SGNH_plant_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051238">
        <property type="entry name" value="GDSL_esterase/lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035669">
        <property type="entry name" value="SGNH_plant_lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45650">
        <property type="entry name" value="GDSL-LIKE LIPASE/ACYLHYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45650:SF50">
        <property type="entry name" value="TRIACYLGLYCEROL LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041935701" evidence="8">
        <location>
            <begin position="20"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7727376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="359" mass="39920" checksum="2ABAD82EE3113CF9" modified="2024-05-29" version="1">MVTTTQIMVFMMLAATLLANCEPQVPCYFIFGDSLMDNGNNNQLLTQAKVNFPPYGIDFPDGPTGRFSNGRNMADVIAQLLGFNKFIPPFATAKGMEVITGVNYASGSAGIRDETAEHLGARISMDKQIKNHWITILRLTYLIGRGSIIKTKDYLKRCIYTVGMGNNDYINNYFLPKYYKTSSVYTPEQYAEALVEQYSRQLQDLYKSGARMFGIFAAGYSGCTPGIMTEFGVNSCVDEVNSAVMLFNSRLNTTLNDLNNKLVDAKFILLDGSFEYPSDLNVTDTPCCVVSSTSGKGQCAPNQVPCSNRQNYYFWDAFHPTERISVLSGTKAYETLSSFYTLETIAMYKDKDTGYISVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G4P6</accession>
    <name>A0AAD5G4P6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transcription factor CBF/NF-Y/archaeal histone domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_020409</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011253">
        <property type="protein sequence ID" value="KAI7728217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G4P6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001046">
        <property type="term" value="F:core promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22906">
        <property type="entry name" value="HFD_DRAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003958">
        <property type="entry name" value="CBFA_NFYB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050568">
        <property type="entry name" value="Transcr_DNA_Rep_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10252">
        <property type="entry name" value="HISTONE-LIKE TRANSCRIPTION FACTOR CCAAT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10252:SF98">
        <property type="entry name" value="TRANSCRIPTION FACTOR CBF_NF-Y_ARCHAEAL HISTONE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00808">
        <property type="entry name" value="CBFD_NFYB_HMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription factor CBF/NF-Y/archaeal histone" evidence="4">
        <location>
            <begin position="133"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="210"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="329"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="16"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="32"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="57"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="97"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7728217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="385" mass="42385" checksum="B79FB1C64A945C59" modified="2024-05-29" version="1" fragment="single">IRNLHTETRNLHTKFDQQATILTSISDKLGIKPPPSPLPQPSPNPTPKPTQATTPTPKVPPPLPQPSLKPTPKPTQAPTPTPKVPPPTGSVIVPSSPNTPPTVPATPPPQPRTKPVTPPSRTIPTLAHNAIVRIKKIMQADEDVGKMAMAVPLLVSKALELFLQDLCDRTYKITLQRGAKTLNSKHLKQCVQSFTLFDFLRDIVGKVPDRDVSDAAGEDRSATKRRKVADGDEKPKREKPVEFYWPESGRVTGMCRSSGKFIPAGTGVDTTAFGYVKHETKPDITDEQKPKAPLDLFEDAESKLNLIPVEKTEDTVKVRNFDLNLDLDENGDTPMGLSTKPTSETKPEEYRGWSLMGVEKMAIDPVKVANLRNSVDEDEEDYDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G560</accession>
    <name>A0AAD5G560_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_015702</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011037">
        <property type="protein sequence ID" value="KAI7729220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G560"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7729220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="175" mass="19137" checksum="D75EDE16DEEEFC09" modified="2024-05-29" version="1">MVEMEPVTGFPLLIPSGDPSVNHYVHGYQVFLHTITIGFELSFHAGESTTQRKDTIEENGSRNVVRHGNLFSSINGNVTISVTANAPIETKVGFRGKRAFQMSAHEVCYPCDPSMKFGSSSSANLATASRSGKYSDGPNLADEWFKQGKYAHSEPLSCEALKEVGKTPHLWCTIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G5A2</accession>
    <name>A0AAD5G5A2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SCP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_002808</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011282">
        <property type="protein sequence ID" value="KAI7727963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G5A2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.33.10:FF:000004">
        <property type="entry name" value="CAP, cysteine-rich secretory protein, antigen 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018244">
        <property type="entry name" value="Allrgn_V5/Tpx1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001283">
        <property type="entry name" value="CRISP-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10334">
        <property type="entry name" value="CYSTEINE-RICH SECRETORY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00837">
        <property type="entry name" value="V5TPXLIKE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00198">
        <property type="entry name" value="SCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="PR-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01009">
        <property type="entry name" value="CRISP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01010">
        <property type="entry name" value="CRISP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0568">Pathogenesis-related protein</keyword>
    <keyword evidence="1" id="KW-0611">Plant defense</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042066378" description="SCP domain-containing protein" evidence="2">
        <location>
            <begin position="24"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="SCP" evidence="3">
        <location>
            <begin position="32"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7727963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="163" mass="18482" checksum="00BA67585ECCF73A" modified="2024-05-29" version="1">MGHWCHISLVLAIFISVLHLSSAQPQYEGEGNSPDDYVNQHNCIRRVLNLQPLKWCDKLAQRAQAWADKRKDCELIHSEGGGENLAYGADLNGLWAVQLWIDERLDYDHSKNECTAMCGHYTQVVWNTTERVGCGKSKCDNGWANFVVCNYDPPGNYIGSYPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G5A3</accession>
    <name>A0AAD5G5A3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_004721</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011036">
        <property type="protein sequence ID" value="KAI7729234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G5A3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37217">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37217:SF1">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7729234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="264" mass="29495" checksum="A869749C44677C26" modified="2024-05-29" version="1">MRVVMNSMFSSSSLSCLPSLCHNPKSYKQLPHFSFTNHWLSSLSIQSTFTSLKPTVCAVTPSEGTVPVINFEDFVEKDWSFLDADDISTDELYKQNIDRIISAGKVGEDSKILISTGSEGFVDRVVDTCSCKQLLVVHDSLFTLACIKEKYDTVKCWQGELIFVPDKWAPFDVVFLYFLPALSFELNQIFGALSKVCSQGARIVISHPKGREMLEKQKAEYSDVVVSGLPDKSTLESVASDHSFTMVDFVDEPGFYIAVLEHTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G5R6</accession>
    <name>A0AAD5G5R6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_001049</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011193">
        <property type="protein sequence ID" value="KAI7728531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G5R6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7728531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="61" mass="6802" checksum="FC9D4FEDEACEA926" modified="2024-05-29" version="1">MSGIKYSDIHNGASSLKGGLEYGLINWVMVELSHLATRHALRVYVGGLPPLARSKFYIYLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G6B5</accession>
    <name>A0AAD5G6B5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_032266</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Co-chaperone that regulates diverse cellular pathways, such as programmed cell death and stress responses.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010824">
        <property type="protein sequence ID" value="KAI7730209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G6B5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000774">
        <property type="term" value="F:adenyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000298">
        <property type="entry name" value="BAG family molecular chaperone regulator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.120">
        <property type="entry name" value="BAG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039773">
        <property type="entry name" value="BAG_chaperone_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036533">
        <property type="entry name" value="BAG_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003103">
        <property type="entry name" value="BAG_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12329:SF43">
        <property type="entry name" value="BAG DOMAIN, UBIQUITIN-RELATED DOMAIN PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12329">
        <property type="entry name" value="BCL2-ASSOCIATED ATHANOGENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02179">
        <property type="entry name" value="BAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00264">
        <property type="entry name" value="BAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63491">
        <property type="entry name" value="BAG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51035">
        <property type="entry name" value="BAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="4">
        <location>
            <begin position="51"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="BAG" evidence="5">
        <location>
            <begin position="144"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="228"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00058673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7730209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="309" mass="34643" checksum="670C56BC1125B087" modified="2024-05-29" version="1">MPMRVKMTELNSVRSATKDGGGDELEVRPGGMLVQKRHSDDALNNIPPRTIKVRVKYESIYHEMIINSTATFGELKRMLSGPTGLHHEDQKIIYKDKERASKTFLDVVGVKDRSKMVLMDDPVSREKRYVEARRNAKVAKAAKAISDIGLEVDRLGGQVLALKSVISKGGKVADEMMSNLMELLMNLLLRLDDVKVDGDVNQQKRFQVEKVQKLVETLDVFKIKNNVGNKGNNNEEHTHGQSVRKFAPVSAASQQQEVSSKNTSRGVVVTTQWETFDSFPIIIPAPPYKSTSITGDTIQHNFNWDLLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G6Q9</accession>
    <name>A0AAD5G6Q9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_010256</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011006">
        <property type="protein sequence ID" value="KAI7729418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G6Q9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072344">
        <property type="term" value="P:rescue of stalled ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16615">
        <property type="entry name" value="RING-HC_ZNF598"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057634">
        <property type="entry name" value="PAH_ZNF598/HEL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041888">
        <property type="entry name" value="RING-HC_ZNF598/HEL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056437">
        <property type="entry name" value="Znf-C2H2_ZNF598/HEL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044288">
        <property type="entry name" value="ZNF598/HEL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22938:SF0">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE ZNF598"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22938">
        <property type="entry name" value="ZINC FINGER PROTEIN 598"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23202">
        <property type="entry name" value="PAH_ZNF598"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25447">
        <property type="entry name" value="RING_ZNF598"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23230">
        <property type="entry name" value="zf-C2H2_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="10">
        <location>
            <begin position="5"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="309"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="530"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="588"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="603"/>
            <end position="617"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7729418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="666" mass="73854" checksum="FA9622B1F58A04A3" modified="2024-05-29" version="1">MDDTCAVCADSLEWVAYGRCGHKDVCSTCVARLRFICNDQRCCICKSDSSIIFVTKALGDYTKTISDFSVFPSENKEGRFGTYMYHEDTQSYFDDLDHYNMIRAMCRLSCSVCDKMEDNSGNKSKRKARFRDINQLKGHLFHQHKLMMCSLCLEGRKIHFRQEHFLCEDEACLTKKFIVFPSEAEMKVHNAQEHGGRLSRSKRNAVLQLPTSFTYGRNNEHDNRRGRRNTFLRDLPESELSRAIEGRETSNSNSRFLEASSSRGTVLSNNNINDVIPLVQPFESLAADSDPPSRYFTAVSHRARNTPLEESAFPPLSTARGASAQNPGGLPRNTMSENLRRKNKKKVNVLNTGNAWPAATRRKPTINLVAVPSRISHSTSAPNLVDVGSVSDFPPVSTLQTRTGSGIGPSTKSVVEVHAANKSMVDKIRAALDNNQDRFTSFKEVSAQYRQGAIDAETYLVYVDQFGLSHLVLDLARLLPDPHKEKELIAVYNANRAWKHKGKSVANNKISTNKLTDNILTTVRELQLNYRPPDDGVETLSKDGYRAANKGKSKVEVDDRTSPGSQLVIKPAKSEGDSAGGEGEGKSKQRKKTSKFLRVRLGNGSQASLDLNNSNGASDAPLAKGESGGKAEQETSDGIPVRGVWRNGGGHKLIVKDLKAPRVIHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G9E9</accession>
    <name>A0AAD5G9E9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Small ribosomal subunit protein uS2c</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_023605</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009982">
        <property type="protein sequence ID" value="KAI7733452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G9E9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01425">
        <property type="entry name" value="RPS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001865">
        <property type="entry name" value="Ribosomal_uS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005706">
        <property type="entry name" value="Ribosomal_uS2_bac/mit/plastid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023591">
        <property type="entry name" value="Ribosomal_uS2_flav_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12534">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S2 PROKARYOTIC AND ORGANELLAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12534:SF0">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00318">
        <property type="entry name" value="Ribosomal_S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52313">
        <property type="entry name" value="Ribosomal protein S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00035155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7733452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="84" mass="9441" checksum="9800CF94DE1F48EA" modified="2024-05-29" version="1">MEAGVHFGHGTRKWNPKMAPYIFAKRKGIHITNLTRTARFLSEAWDLDFDAVSRGKQFLIVGTKNKEVDSVAWAAIYKGSVSLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GCC1</accession>
    <name>A0AAD5GCC1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_026980</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the aromatic acid exporter (TC 2.A.85) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009327">
        <property type="protein sequence ID" value="KAI7736284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GCC1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015743">
        <property type="term" value="P:malate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034220">
        <property type="term" value="P:monoatomic ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020966">
        <property type="entry name" value="ALMT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31086">
        <property type="entry name" value="ALUMINUM-ACTIVATED MALATE TRANSPORTER 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11744">
        <property type="entry name" value="ALMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0407">Ion channel</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="45"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="76"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="101"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="124"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="156"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="187"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7736284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="420" mass="46343" checksum="7409EB7B46ADF951" modified="2024-05-29" version="1">MSTTIDIPIENANANENSSNSKKQSFVYSFASFIEKQFGQDMKKIIHSIKVGIALVLVSLLYLLDPLFEQVGENAMWAIMTVVVVFEFYAGALLSKGLLRGLGTILGGGLGCLAAILADDLGKIGNAAVVGSSVFVFGAAATYCRTIPTIKSKYDYGFMIFILTFNLVAVSGLRADKVIELARERLSTIGMGFAVCIFTSLLVFPMWASDELHRSTSSKFDKLACCIEDCMKAYFNIVDEKESMPKINVKDCKAVLHSKSSDESLANFAKWEPWHGRFGFYYPWDKYLQVGELIRELASLILSLKECLESPLQPSTQLQHEIKEPCKSVGLSLGLILRELGRSIRNMKRCQATVIIQELQLVKQELNLLSTSPKLKGIANVESLATANMLFLLMKIVDKVEVLAKEVEALGEVAGFQLSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GDG2</accession>
    <name>A0AAD5GDG2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Late embryogenesis abundant protein LEA-2 subgroup domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_009768</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008846">
        <property type="protein sequence ID" value="KAI7738237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GDG2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055301">
        <property type="entry name" value="Lea14-like_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31852">
        <property type="entry name" value="LATE EMBRYOGENESIS ABUNDANT (LEA) HYDROXYPROLINE-RICH GLYCOPROTEIN FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="113"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="54"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7738237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="303" mass="32918" checksum="F6D58A1C39BC6C6A" modified="2024-05-29" version="1">MSMHAKTDSEVTSLTASSPTRSPQRPVYYVQSPSRDSHDGGKTTNSFHSTPVLSPSGSPSRQSRNSSSTRYSGSVRPGSRKGGQVKGEKGFDAIEEEGLYDEEGGRGIPRRCYFPAFVVGFFVLFTFFALVLWGAARPQKPVVTMRSIAFDQFVVNAGADASGVATEMVTLNATVKFNFRNRGTFFGVHVSSTPLDLAYTELTLATGTVKKFYQSRKGHRTVSVNVQGVRIPLYGGGVNWSSENGKLTAPVPLNLNFTVKAKAYVLGKLVKPKFHRKVSCAVVYSPTKVINKPISLKNSCTVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GDL6</accession>
    <name>A0AAD5GDL6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_013278</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008817">
        <property type="protein sequence ID" value="KAI7738417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GDL6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01396">
        <property type="entry name" value="MeCP2_MBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.890.10">
        <property type="entry name" value="Methyl-cpg-binding Protein 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001739">
        <property type="entry name" value="Methyl_CpG_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011124">
        <property type="entry name" value="Znf_CW"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12396">
        <property type="entry name" value="METHYL-CPG BINDING PROTEIN, MBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12396:SF50">
        <property type="entry name" value="ZINC FINGER, CW-TYPE, DNA-BINDING DOMAIN PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01429">
        <property type="entry name" value="MBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07496">
        <property type="entry name" value="zf-CW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00391">
        <property type="entry name" value="MBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50982">
        <property type="entry name" value="MBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51050">
        <property type="entry name" value="ZF_CW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CW-type" evidence="11">
        <location>
            <begin position="107"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="MBD" evidence="10">
        <location>
            <begin position="172"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="26"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7738417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="331" mass="37089" checksum="2EBF1A1924B0FA37" modified="2024-05-29" version="1">MQSRSPKITIKMGGKRPDMTPPAVKSIDETPEKDQSAPHQGPSALSNNDRKETDENALDGSSSEYSSDDEDKNKQMVVYDHSLNGSGVIKAVKNPKSNKPRSASKKPPEVGAFTVQCANCLKWRLIPDQEKYEVIREHITDQPFLCETTRQWNRVVSCDDPTDIEQDGTRIWAIDKPDIARPPPGWKRILRLRNEGSSKFADVYYTSPTGAKLRSLPDVFRYFESHPEHSQGLGPGQFSFQIPRPLEKSYVKKRPAQMAAPKTPETLEPSPVEPLAWAGPAENLNLQLAEPLSWAGPVEDKDGMPAYLKTPEFDFENQAAKKAKRDEVNHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GFT3</accession>
    <name>A0AAD5GFT3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_033864</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GASA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008568">
        <property type="protein sequence ID" value="KAI7739779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GFT3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003854">
        <property type="entry name" value="GASA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23201">
        <property type="entry name" value="EXTENSIN, PROLINE-RICH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23201:SF100">
        <property type="entry name" value="GIBBERELLIN REGULATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02704">
        <property type="entry name" value="GASA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042074960" evidence="2">
        <location>
            <begin position="22"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7739779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="97" mass="10311" checksum="B7D287196EDF0B4B" modified="2024-05-29" version="1">MNHLPIVALLIAAFLLSSATAVDLSGPSPAPTSDPLGCDAKCAYRCSRSGWRDRCLKYCGICCGKCQGCVPAGPYADKAQCPCYRDLKNPKGRDKCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GHC0</accession>
    <name>A0AAD5GHC0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_029335</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ARG7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008543">
        <property type="protein sequence ID" value="KAI7739933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GHC0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009733">
        <property type="term" value="P:response to auxin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003676">
        <property type="entry name" value="SAUR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31929">
        <property type="entry name" value="SAUR-LIKE AUXIN-RESPONSIVE PROTEIN FAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02519">
        <property type="entry name" value="Auxin_inducible"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7739933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="96" mass="10925" checksum="9321EFAAC662F000" modified="2024-05-29" version="1">MGLHKFPKTKYTKLAIGRYQSSSPVNDVPKGHFSVYVGKNNLKRFIVPLAYLKHPSFQTLLNLAEEEFGYAHPMGGLTFPCKEETFLELVENIHLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GLN4</accession>
    <name>A0AAD5GLN4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GH18 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_004152</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006720">
        <property type="protein sequence ID" value="KAI7747582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GLN4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004568">
        <property type="term" value="F:chitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050542">
        <property type="entry name" value="Glycosyl_Hydrlase18_Chitinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45708">
        <property type="entry name" value="ENDOCHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45708:SF49">
        <property type="entry name" value="ENDOCHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GH18" evidence="3">
        <location>
            <begin position="1"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7747582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="217" mass="23246" checksum="51F5EC0230D2C620" modified="2024-05-29" version="1" fragment="single">WVQMPEALLSTGVKTGFYNNPPCQYAGGITNLEDAWKQWTSEIPATKIFLGLPASPSAAGSGYIPVGDLTSKVLPAIKGSTKYGGVMLWDKYYDDQTGYRIKVMLTLGGAAGSYYLTSAADAKQVATYLWNNFLGGKSSNRPLDAWKQWTSEIPATKIFLGLPASPSAAGSGYIPVGDLTSKVLPAIKGSAKYGGVMLWDKYHDNQTGYSSSIKSHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GNV6</accession>
    <name>A0AAD5GNV6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">phytol kinase</fullName>
            <ecNumber evidence="12">2.7.1.182</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">M8C21_027512</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">AA19_3_7</strain>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>phytol + CTP = phytyl phosphate + CDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38055"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17327"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:58069"/>
            <dbReference type="ChEBI" id="CHEBI:75483"/>
            <dbReference type="EC" id="2.7.1.182"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="11">Cofactor biosynthesis; tocopherol biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the polyprenol kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.182" evidence="12"/>
    <dbReference type="EMBL" id="JAMZMK010006268">
        <property type="protein sequence ID" value="KAI7749847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GNV6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031969">
        <property type="term" value="C:chloroplast membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010276">
        <property type="term" value="F:phytol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010189">
        <property type="term" value="P:vitamin E biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039606">
        <property type="entry name" value="Phytol/farnesol_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32523:SF8">
        <property type="entry name" value="DOLICHOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32523">
        <property type="entry name" value="PHYTOL KINASE 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0934">Plastid</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="29"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00039024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAI7749847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="106" mass="11759" checksum="2E3294080077833D" modified="2024-05-29" version="1">MMCGGDGIADIMGRRFGIHKIPYNERKSWVGSISMFIVGFLISIGMLYYFSKFGYFELDLVWTIERVALVAMVATVVESLPTKGNLDDNVSVPLVSMVTAYLSFGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GP40</accession>
    <name>A0AAD5GP40_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glutathione S-transferase</fullName>
            <ecNumber evidence="5">2.5.1.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_007956</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Is involved in the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 5">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
            <location evidence="5">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the GST superfamily. Tau family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="5"/>
    <dbReference type="EMBL" id="JAMZMK010006893">
        <property type="protein sequence ID" value="KAI7746818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GP40"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009407">
        <property type="term" value="P:toxin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03185">
        <property type="entry name" value="GST_C_Tau"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03058">
        <property type="entry name" value="GST_N_Tau"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000044">
        <property type="entry name" value="Glutathione S-transferase GSTU6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000016">
        <property type="entry name" value="Glutathione S-transferase U9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045074">
        <property type="entry name" value="GST_C_Tau"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045073">
        <property type="entry name" value="Omega/Tau-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11260:SF773">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE U26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11260">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE, GST, SUPERFAMILY, GST DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14497">
        <property type="entry name" value="GST_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13417">
        <property type="entry name" value="GST_N_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01152">
        <property type="entry name" value="Main.3:_Omega-_and_Tau-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1" id="KW-0216">Detoxification</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="6">
        <location>
            <begin position="5"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="7">
        <location>
            <begin position="91"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU369102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7746818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="230" mass="26333" checksum="6B05240130F366A3" modified="2024-05-29" version="1">MGNESEIRLLGTAASPFVNRVQFALNLKSIKYEYTEEYLARKSELLLKSNPVHKKVPVLLHADKPPICESQIIIEYLDEIQPDLFQLLPSASLDRANCRFWAAYIDNKFFPLYEELRRTTKKAGQEAIKEQMIEATKLLEEAFVKSSNGKAYFGGDQVGYLDVVLGCFVRWIKHVDELNDFNTFDEVRVPELAKWAKNVSSHEALKSVTPGIETLVSFFGLLHIYKPPYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-06-18" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GPT1</accession>
    <name>A0AAD5GPT1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">Pectinesterase</fullName>
            <ecNumber evidence="4 10">3.1.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_025273</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 10">
            <text>[(1-&gt;4)-alpha-D-galacturonosyl methyl ester](n) + n H2O = [(1-&gt;4)-alpha-D-galacturonosyl](n) + n methanol + n H(+)</text>
            <dbReference type="Rhea" id="RHEA:22380"/>
            <dbReference type="Rhea" id="RHEA-COMP:14570"/>
            <dbReference type="Rhea" id="RHEA-COMP:14573"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17790"/>
            <dbReference type="ChEBI" id="CHEBI:140522"/>
            <dbReference type="ChEBI" id="CHEBI:140523"/>
            <dbReference type="EC" id="3.1.1.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the pectinesterase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the PMEI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.11" evidence="4 10"/>
    <dbReference type="EMBL" id="JAMZMK010006074">
        <property type="protein sequence ID" value="KAI7750867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004857">
        <property type="term" value="F:enzyme inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030599">
        <property type="term" value="F:pectinesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042545">
        <property type="term" value="P:cell wall modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045490">
        <property type="term" value="P:pectin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd15798">
        <property type="entry name" value="PMEI-like_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.10:FF:000001">
        <property type="entry name" value="Pectinesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.40">
        <property type="entry name" value="Invertase/pectin methylesterase inhibitor family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035513">
        <property type="entry name" value="Invertase/methylesterase_inhib"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033131">
        <property type="entry name" value="Pectinesterase_Asp_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000070">
        <property type="entry name" value="Pectinesterase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006501">
        <property type="entry name" value="Pectinesterase_inhib_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31707">
        <property type="entry name" value="PECTINESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01095">
        <property type="entry name" value="Pectinesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04043">
        <property type="entry name" value="PMEI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00856">
        <property type="entry name" value="PMEI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101148">
        <property type="entry name" value="Plant invertase/pectin methylesterase inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00503">
        <property type="entry name" value="PECTINESTERASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0063">Aspartyl esterase</keyword>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="5 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pectinesterase inhibitor" evidence="11">
        <location>
            <begin position="1"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="323"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7750867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="486" mass="54330" checksum="32A71ADFDBA8B1FC" modified="2024-05-29" version="1" fragment="single">ETGGDINYWCETTPHPQQCKFFLSRGVALERAQDVQTCSTNLRNKCRSKRKKAVWLDCNKLLNGTIYQLSQTLNGLKHNLSSDFDAXXXXLSAALTNLETCFSGSRDFNLTNLLSPLKSHNLTEMISNSLAINQFFVKQKLESQDLNDDYPSWVTPGDRKLLQSNSIHMKANIIVSQARGSRFRTIQSALKFAAGLKRGNKRYIIYIRRGVYRENLEIGNNLNNIMFLGDGARYTIITGSRSVGGGFTTYSSATVGVDGIGFIARGITFRNTAGPAKSQAVAFRSSSDLSVYYACSFEGYQDTLFVLAQRQFYKMCYIYGTIDFIFGNAAVVFQNCMIMARRPLKGQANVITAQGRGDPFQNTGISIHNSRVMPAPDLKPVMRSVQTYLGRPWQEYSRTVYMKTFMDSHINPSGWSPWDNSNFAQKTLVYGEYGNFGPGSSTRNRVKWPGYQVIRSASVASQYTVERLIAGRSWLPATGIPFTPGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GQ01</accession>
    <name>A0AAD5GQ01_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PB1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_009925</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006417">
        <property type="protein sequence ID" value="KAI7749019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GQ01"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06410">
        <property type="entry name" value="PB1_UP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053198">
        <property type="entry name" value="Gynoecium_Dev_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000270">
        <property type="entry name" value="PB1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31066">
        <property type="entry name" value="OS05G0427100 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31066:SF88">
        <property type="entry name" value="PB1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00564">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00666">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PB1" evidence="2">
        <location>
            <begin position="42"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="390"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="454"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="398"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="460"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7749019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="473" mass="51336" checksum="5628608C7333DFF1" modified="2024-05-29" version="1">MDPPTTTTTTVVEPPHSHSLPPLPTSKLRLMCSYGGHIIPRPHSKSLCYAGGDTRIVAVDRRTTAATISSLSHHLSRTLFNNRPFTLKYQLPNEDLDSLISITTDEDLHNMLEEHDRIVSSPTPSRIRLFLFPTRPESVGSLLLDPKSESWFSDALKSTMIINRGLSDGSGLGNGLMGLECLDGNGDSLVNSGDSGKCGGGDSGSVPESMVLETCSSFGSINSSVSASNLVPVGVVNEDGLEKKVRVAAPVSVESDASARMYQDQMLRESESIIYNPSSPVQMHKNIQVSGYQMPYMPDQRQQPVQYIHSASPHYIQYPTGPALPISSYYPMYVPYQQPPPPLQNQPYPVYVMPVGPTDQAPNISTPTHIGNTHVAYKEVRVPQPPVERAAVAPQPTPSNRVPTPSGQSVGPTVTPTPTYENEYDNDLVYAQIYKSQASAPAFPPQMFDAVKKAEHVPLSDSSTQVPIGTSQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GQB1</accession>
    <name>A0AAD5GQB1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tropinone reductase-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_018169</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005977">
        <property type="protein sequence ID" value="KAI7751120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GQB1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045000">
        <property type="entry name" value="TR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42898:SF6">
        <property type="entry name" value="NADP-DEPENDENT MANNITOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42898">
        <property type="entry name" value="TROPINONE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7751120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="166" mass="17849" checksum="B8841B0E5149149F" modified="2024-05-29" version="1" fragment="single">INNVGTNIKKPTMEYTAQEYSMIMSTNLESSYHMCQLAHPLLKASGLASIVFISSVAGLVHMSSGSIYGATKGAMNQLAKNLACEWAKDNIRVNSVAPWYINTSLVEHVLSDKQFLDKVASRTPLKRPGEANEVSSMVAFLCLPAASYITGQTIAVDGGFSVNGFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GR37</accession>
    <name>A0AAD5GR37_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_006316</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006450">
        <property type="protein sequence ID" value="KAI7748903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GR37"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42678">
        <property type="entry name" value="AMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42678:SF34">
        <property type="entry name" value="OS04G0183300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042003427" evidence="1">
        <location>
            <begin position="20"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7748903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="210" mass="23032" checksum="24D6D845EEB2CB58" modified="2024-05-29" version="1">MFPICLNLSLNLLITFISCILHNTRQKGAILIENLEISNFSDIVSMLGGEYIAMTAEFKLALNAYLKQLVVSPVRSLADVIAFNKKFADLEKINDYPQDLFLEAEKTNGIGKLEKDALMNLAKASKYGFEKLMNDNKLDALVTPYYTVSGVLAIGGYPGISVPSGYDKDGGPYGICFGGLKGSEPTLIEIAYGFEQATKFRKPPTIKNQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GSB6</accession>
    <name>A0AAD5GSB6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">FYVE-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_020280</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005523">
        <property type="protein sequence ID" value="KAI7753175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GSB6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00065">
        <property type="entry name" value="FYVE_like_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13365">
        <property type="entry name" value="PH_PLC_plant-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.30:FF:000028">
        <property type="entry name" value="PH, RCC1 and FYVE domains-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013591">
        <property type="entry name" value="Brevis_radix_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058923">
        <property type="entry name" value="RCC1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051210">
        <property type="entry name" value="Ub_ligase/GEF_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22870">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22870:SF91">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION (RCC1) FAMILY WITH FYVE ZINC FINGER DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08381">
        <property type="entry name" value="BRX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16457">
        <property type="entry name" value="PH_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25390">
        <property type="entry name" value="WD40_RLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00633">
        <property type="entry name" value="RCCNDNSATION"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00626">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="repeat" description="RCC1" evidence="6">
        <location>
            <begin position="266"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="6">
        <location>
            <begin position="328"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="6">
        <location>
            <begin position="380"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="6">
        <location>
            <begin position="435"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="6">
        <location>
            <begin position="498"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="domain" description="FYVE-type" evidence="9">
        <location>
            <begin position="553"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="184"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="700"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="842"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="733"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="184"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="200"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="701"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="854"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="885"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="980"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7753175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="980" mass="106598" checksum="9400EE4F0DC02A30" modified="2024-05-29" version="1" fragment="single">ALIALKKGAHLLKYGRKGKPKFCPFRLSHGLILWFISTRKYAQLIRELLLVSSAEIALCSFPSCHLTFANGWNLNPQDESSLIWISDSGERSLKLASVSRIIPGQRTAVFRRYLRPEKDYLSFSLIYNNGKRSLDLICKDKVEAEVWIAGLKALISSLQEGRLKIDGWSDEGLYLDDNRDLASNSASDSSISAPRDFSSPDISISSNPTTSPKSHPPGLERSYAAALDQTNMQLKGSSSDAFRVSISSAPSTSSHGSGLDDSDSLGDVYIWGEVICDNVVKLGPDQNVNLSSTRTDVLLPRPLESNLVLDVSHIACGVRHAALVTRQGELFTWGEEFGGRLGHGVGKDESQPRLVESLAVSNIDFVACGEFHTCAVTSSGEMYTWGDGTHNIGLLGHGTDVSQWIPKRISGPLEGLQVASVACGPWHTALITSTGQLFTFGDGTFGVLGHGNHENVSYPKEVESLSGLRTVAVACGVWHTAAVVDVIVAQSSPSVLSGKLFTWGDGVNNRLGHGDEESRLKPTCVPALIDYNFHKVACGHSLTVGLTTSGHAFGFTRKRHNCYNCGLVHCHSCSSKKAPRAALAPNPGKPYRVCDSCYTKLSKMAETRITNRQNVKPRLSGENKDKLDKAELKLAKSGTPSNQDLIQLLDNKAAKQGKKGDAFLGQSTQGPSLMQLKDVMLLGDFLQAIPKSINIPSGITSRSVSPISRNPSPPRSGTHVPTTSGLSFSKGFVDSLKKTNELLNQEVLKLRGQVDILKRQCSIQECELQKSRKAAQEAMVLAAEESAKFKASQDVIKSLAAKLKDKAESTKSENGLEQNGVIQYAYVNGDDTLLSNVSLGPTETISRTEEPMQYDSSSPKSRTSNKNNECLNGVGVQLGSNNYVESKDYSPPHDEDNNFEPRSPVSSVGTPATTTNHVEAEWSAVQLRWAEFENRTGTVELVNKRTNPISRRRFGEQQAETWLSENCEKVCERYNVIGSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GSJ3</accession>
    <name>A0AAD5GSJ3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_013991</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006149">
        <property type="protein sequence ID" value="KAI7750493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GSJ3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34956">
        <property type="entry name" value="OS05G0397300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34956:SF2">
        <property type="entry name" value="OS05G0397300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7750493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="88" mass="10135" checksum="5A53D4C65D7C6E00" modified="2024-05-29" version="1">MEVVEDDLFFEDLSKRISLLIMDDDEDPTLYCPPVSFQVLSQTIHTNHNQIPSFNEQNGRREIKGTGVFIPQSSTNSRRNKKQVLNVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GSZ1</accession>
    <name>A0AAD5GSZ1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_024899</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006063">
        <property type="protein sequence ID" value="KAI7750898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010333">
        <property type="term" value="F:terpene synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016102">
        <property type="term" value="P:diterpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046246">
        <property type="term" value="P:terpene biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00684">
        <property type="entry name" value="Terpene_cyclase_plant_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.130:FF:000001">
        <property type="entry name" value="Isoprene synthase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.130">
        <property type="entry name" value="Terpene synthase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034741">
        <property type="entry name" value="Terpene_cyclase-like_1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044814">
        <property type="entry name" value="Terpene_cyclase_plant_C1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001906">
        <property type="entry name" value="Terpene_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036965">
        <property type="entry name" value="Terpene_synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050148">
        <property type="entry name" value="Terpene_synthase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005630">
        <property type="entry name" value="Terpene_synthase_metal-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31225">
        <property type="entry name" value="OS04G0344100 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31225:SF9">
        <property type="entry name" value="TERPENE SYNTHASE 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01397">
        <property type="entry name" value="Terpene_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03936">
        <property type="entry name" value="Terpene_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01019">
        <property type="entry name" value="Terpene_Cyclase_Like_1_C_Termi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Terpene synthase N-terminal" evidence="4">
        <location>
            <begin position="66"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="Terpene synthase metal-binding" evidence="5">
        <location>
            <begin position="429"/>
            <end position="648"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7750898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="703" mass="81139" checksum="0478B8A22F833413" modified="2024-05-29" version="1">MTSMCFIPCSVLHYKTCQYVSGQFSIVKPLHLSRSKSNVVITAKFANEVEPVTRRSANYKPSLWSSDEVQSLSSEYIGEDYKARADTLKMAVKMMIHKVGGPLSSLELVDDLQRLGISYHFEDEIKDLMKMIYHNYYKTHDKWNRMDMNLKALGFRLLRQHGYQVPQEIFLNFKDTTQDLKPHLLKDTVAILNLYEASYHSFEDESILDDVRDFTTRYLKENQDKIDESISSLVSHALEFPLHWRVPRVESKWFIELYEKRSDMNPTIVELAKLDFNMVQAFHIEDLKHASRWWSNTRWDKKLTFAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXWTVGYTYQPQFSLGRRNVTKVIAMVTAIDDIYDVYGTLNELEQFTDVVSRWDVNAIQELPDYMRICFLGFYNTINEIAYNTLTDSGVFILPYLKKAWADLCKSYLVEARWYYSGHVPTLQEYLHNACVTIATPLVMTHNNFSTSIGSTEEILQIFEESKNIVHHASLIFRLADDLGTSSDEMQRGDIPKSIQCYMHETGATEDEARRYIKNLIMKSWKKLNKEQSSAKSQLLRGFIDYAMANVRMAQFMYGKGDGHGRPNVIKSHVLSLLFNPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GTB0</accession>
    <name>A0AAD5GTB0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">P-type ATPase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_026976</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010003807">
        <property type="protein sequence ID" value="KAI7754390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GTB0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140326">
        <property type="term" value="F:ATPase-coupled intramembrane lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000025">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF217">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="162"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="219"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="600"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="629"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="675"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="711"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="741"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="770"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase C-terminal" evidence="8">
        <location>
            <begin position="564"/>
            <end position="799"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF16212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7754390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="827" mass="93052" checksum="D62818D73C2FB916" modified="2024-05-29" version="1">MSYVLVNDRFEQKRWKDIQVGEVVKVLADETIPCDIVLLSTSDLTGVAYIQTINLDGESNLKTRYAKQETLSRVPEKDIISGLIKCEKPNRNIYGFQGNMEIDGKRVSLGPSNIILRGCVIKNTDWAVGVAVYAGRETKAMLNNSGAPSKRSRLETHMNQEIMLLSFFLVTLCVVVSACAGVWLKRHQDELDMMQFYRRKDYSEGEEENYNYYGLGLEIFFSFLMSVIVFQIMIPISLYISMELVRVGQAYFMIHDDKMFDAASNTRFQCRALNINEDLGQINKDIYDFFLALAACNTIVPIVVNTSNPNEKLIDYQGESPDEQALVYAAAAYGFMLIERTSGHIVIDIQGERQRFNVLGMHEFDSDRKRMSVILGYPDGTVKVFVKGADTTMFKVIDESLNLDVVRATETHLHSYSSIGLRTLVVGMRALSVSEFDTWQTSYEIASTALMGRAGLLRKVAIKLENNLSLLGASAIEDKLQQGVPEAIESLRKADIKLFELASKCAVVLCCRVAPLQKAGIVALIKKRTDDMTLAIGDGANDVSMIQMADVGVGISGQEGRQAVMSSDFAMGQFRFLVPLLLVHGHWNYQRMAYMILYNFYRNALFVLVLYVLYTGFTLTTAITEWSSVLYSIIYTAIPTIVIGILDKDLSRKSLMTYPQLYGAGQRKESYNLKLFWVTMVDTLWQSVVAFFIPVYAYWKSDIDSSSLGDLWTLAVVFMVNIHLAMDVIRWTWISHASIWGSIIATCICVIVIDAIPVLPGAIFNLASSGLFWICVLGIPIISMIPRFVVKMFMQHCKPSDIQIAREAEKFRNSMEITRSGEIELNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GU25</accession>
    <name>A0AAD5GU25_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_016680</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000976">
        <property type="protein sequence ID" value="KAI7755607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GU25"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025886">
        <property type="entry name" value="PP2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32278">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32278:SF15">
        <property type="entry name" value="F-BOX PROTEIN PP2-B13-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14299">
        <property type="entry name" value="PP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7755607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="275" mass="32485" checksum="A45AF8EB891A23E6" modified="2024-05-29" version="1">MLYESEDYEIWEPKLPKDYKEIIHSISKHLEINNSLETKKDIYNMLTKGVLLQDIKVLFSLGSKGERNELVSARMFSRKNHTAHKWLSVPESRFPKVLRMHDSSNLKIQIKIRTQFLPPSANYKVFIIFRFCTPIKSRAKRIYVNLKYKMGNETMHSYFATWREDGWMTIELFRFLNHKKDTDFEVLLESFSRCYSGRHSVKHGGIRKLKEVQQVIKSKSKMDKLSSSKMKVKKDHMTWFSLSEIKREKNEMLSAEKVVYNSSDVAIQSSIMARV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GU43</accession>
    <name>A0AAD5GU43_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phospholipase A2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_030942</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000905">
        <property type="protein sequence ID" value="KAI7755667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GU43"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004623">
        <property type="term" value="F:phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050482">
        <property type="term" value="P:arachidonate secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.90.10">
        <property type="entry name" value="Phospholipase A2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036444">
        <property type="entry name" value="PLipase_A2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37246">
        <property type="entry name" value="OS07G0658000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37246:SF1">
        <property type="entry name" value="PHOSPHOLIPASE A2 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48619">
        <property type="entry name" value="Phospholipase A2, PLA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7755667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="221" mass="25548" checksum="9DA887154E2F834E" modified="2024-05-29" version="1">MMTDKADTSTPLEESPKQEAFDVKFLGMSLTKIVPWAIDTEKIKLPTFLNKKLKKHAQQSRLRNLSEPGLSYSTVRFRPYVCKVPWHTGPRAFLSQLFPRYGHYCGPNWSSGKDGGSLLWDKRPIDWLDYCCYCHDMGYDTHDQAELLKADLAFLECLERPHMATKGDVRVAHLYKTMCISGLRSILIPYRQHLVKLKSGQLYLGFGWLSNMKWKGWNAQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GUG9</accession>
    <name>A0AAD5GUG9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_025503</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005318">
        <property type="protein sequence ID" value="KAI7753699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GUG9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37249">
        <property type="entry name" value="OS03G0206201 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37249:SF3">
        <property type="entry name" value="OS03G0206201 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7753699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="66" mass="7249" checksum="5C6AAA4372B7D62E" modified="2024-05-29" version="1">MKVKENINADHYDQGNASNIDLLDYRPIDPVPSSKASIRHGPIQHDVPLVPYIPKPPPSATNDHGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GVH3</accession>
    <name>A0AAD5GVH3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nucleoside phosphorylase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_026190</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010002850">
        <property type="protein sequence ID" value="KAI7754659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GVH3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009116">
        <property type="term" value="P:nucleoside metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09008">
        <property type="entry name" value="MTAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1580">
        <property type="entry name" value="Nucleoside phosphorylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000845">
        <property type="entry name" value="Nucleoside_phosphorylase_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035994">
        <property type="entry name" value="Nucleoside_phosphorylase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21234:SF19">
        <property type="entry name" value="BARK STORAGE PROTEIN B-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21234">
        <property type="entry name" value="PURINE NUCLEOSIDE PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01048">
        <property type="entry name" value="PNP_UDP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53167">
        <property type="entry name" value="Purine and uridine phosphorylases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042154313" description="Nucleoside phosphorylase domain-containing protein" evidence="1">
        <location>
            <begin position="26"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoside phosphorylase" evidence="2">
        <location>
            <begin position="46"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7754659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="351" mass="38979" checksum="E9D1A3954B7396BA" modified="2024-05-29" version="1">MFRNIFSTFIIYVLIFVTSQEQVNGALHGSTKKMVDQANKNGPYLGLVIPNMFEMNPLLNNPHYKSTKLVIDYAGRRFRFGNIYNKPVILVMSGMGMVNSAIATQLLLSLFEIKGVIHYGIAGNANPNLNIGDVTIAQYWSHSALWNWQRYGDGPEDSLPFEGEGGFTRDIGYLKFGSYSSNGVDDNLLNNVWYQAEEVYPVDSTPEQTQQVFWVPVDSNYLSISKALEKLKLEDCINATTCLSKPPKVSTVQRGTSANIYLDNAAYRSFLYEKFNISPVEMESAGVALICYQQKVPFITFRALSDLAGGGSASSNEADTFSGLSANNSVIVTVEFIKLLAGYNRKMLSYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GVH5</accession>
    <name>A0AAD5GVH5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Beta'-coat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_030309</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000236">
        <property type="protein sequence ID" value="KAI7756895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GVH5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030126">
        <property type="term" value="C:COPI vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000016">
        <property type="entry name" value="Coatomer alpha subunit, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050844">
        <property type="entry name" value="Coatomer_complex_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19876">
        <property type="entry name" value="COATOMER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19876:SF76">
        <property type="entry name" value="WD40_YVTN REPEAT-LIKE-CONTAINING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 7" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="95"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="138"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="181"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="225"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00032920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7756895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="302" mass="34598" checksum="D1459B946703CB35" modified="2024-05-29" version="1">MGSTIEIKKTFELRSERVKSIDLHPTTPWVLLGMHSGSVCIWNHHSQVTEKTFEIANTRVRSAIFIASKEWIVIGADDGFIRVYNYNTMENIIEFKAHSDFVRSLIAHPSKSYLLSASDDKHIKLWDWDKDWECTKTFEGHEHYVMQLAFNPRDTNVFASVSLDHTIKFWDLGSMDLRSSINGHTEGLNSVDFFKDEGKFYVLTGSDDHTAKVWDYESMTCVKTLEGHTHNVTSFQCVKLNSSFIFTGSEDQTVRVWNAKTYKLDHVFTSELGRVWTIGFIEDSSKIILGCDEGLIVGQVTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GWD2</accession>
    <name>A0AAD5GWD2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Zinc finger protein BRUTUS</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">M8C21_032570</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">AA19_3_7</strain>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Probable E3 ubiquitin-protein ligase that may regulate the response to iron deficiency and thus contributes to iron homeostasis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Binds zinc and iron ions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000020">
        <property type="protein sequence ID" value="KAI7758172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GWD2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098711">
        <property type="term" value="P:iron ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034756">
        <property type="term" value="P:regulation of iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12108">
        <property type="entry name" value="Hr-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd16464">
        <property type="entry name" value="RING-H2_Pirh2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.520:FF:000009">
        <property type="entry name" value="Zinc finger protein BRUTUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000208">
        <property type="entry name" value="Zinc finger protein-related isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.520">
        <property type="entry name" value="nmb1532 protein domain like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012312">
        <property type="entry name" value="Hemerythrin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039512">
        <property type="entry name" value="RCHY1_zinc-ribbon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008913">
        <property type="entry name" value="Znf_CHY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037274">
        <property type="entry name" value="Znf_CHY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017921">
        <property type="entry name" value="Znf_CTCHY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037275">
        <property type="entry name" value="Znf_CTCHY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21319:SF0">
        <property type="entry name" value="AND RING FINGER DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G08900)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21319">
        <property type="entry name" value="RING FINGER AND CHY ZINC FINGER DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01814">
        <property type="entry name" value="Hemerythrin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05495">
        <property type="entry name" value="zf-CHY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14599">
        <property type="entry name" value="zinc_ribbon_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161219">
        <property type="entry name" value="CHY zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161245">
        <property type="entry name" value="Zinc hairpin stack"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51266">
        <property type="entry name" value="ZF_CHY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51270">
        <property type="entry name" value="ZF_CTCHY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 16" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CHY-type" evidence="18">
        <location>
            <begin position="945"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="domain" description="CTCHY-type" evidence="19">
        <location>
            <begin position="1017"/>
            <end position="1080"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="17">
        <location>
            <begin position="1081"/>
            <end position="1123"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="20">
        <location>
            <position position="1197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00053847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00063786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAI7758172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="22">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1197" mass="135723" checksum="4783534BF58D0AB6" modified="2024-05-29" version="1" fragment="single">HCSLLLNNIMATPLTELGVGGGVAVMATMNQIESSSNNNNNKHSKKQRSPIHIFLYFHKAIRSELDALHRSAIDFVNNNLVDIEPLLKRYHFLRSIYQHHCNAEDEVIFPALDIRVKNVARTYSLEHEGESVIFDQLFALLDSNMQNEESFRRELASCTGALQTSISQHMSKEEEQVIPLLVEKFSFEEQASLVWQFLCSIPVNMMAEFLPWLSASVSSDERQEMRSSLCRVIPEEKLLQQIIFTWMDGVNMLKKRKNLEDDVTNQCSPISEKNCACSSSWSIKQETSLRSSDSPLDRPVDEILHWHKAIEKELVDIADAARRIQLSGDFSDLSAFNKRLQFIAEVCIFHSIAEDKVIFPAFDAELSFAQEHAEEESEFDKFRCLIESIENDGANSSSSEFCSKLCSHANHIMAIIGNHFKNEELQVLPLARKHFSPKKQRELLYKSLCVMPLRLIECVLPWLVGSLTEEDAKSFLHNMRMAAPASDTALVTLFLGWACKGWPRETCLSSNATGCCSAKSFLEENNGSGPPFCPCNLPTKRSNTTSRKESNGFGIPGTLTGQVKKSCCVPGLGMNSNKLGTGSTKSLRSFSFGPSAPLSSSVFNWETGISLIDEGTGRPMDTIFKFHKAIRKDLEFLDVESAKLSESNESFLHQFDGRFRLLWGLYRAHSNAEDDIVFPALESKETLHNVSHSYTLDHKQEEKLFEDISMSLFELCELHDKNNSISSKCRNNLRNYAELATKVQGMCKSIRVTLDQHILREELELWPLFDIHFSVEEQDKLVGRIIGTTGAEVLQSMLPWVTSVLTQEEQDRMMDTWKQATKNTMFTEWLNEWWEGSSPSSDASPSYSDKNISQGSELHEALDPNGYTFKPGWKDIFRMNENELASEMRKVSSDPALDPRRKAYLRQNLMTSRWIAAQQKSPQGRESDKTDGEGLYGCSPSFRDTEKQIFGCEHYKRNCKLRAACCQKLFTCRFCHDNVSDHTMDRKATTEMMCMNCLKVQPVGPVCSTPSCNGLSMAKYYCSYCKFFDDERMVYHCPFCNLCRLGKGLGVDFFHCMTCNYCLGIKLLDHKCREKGLETNCPICCDFLFTSSAAVRALPCGHFMHSACFQAYACTHYICPICSKSMGDMSVYFGMLDALMASEVLPEDYRNRRHDILCNDCGKKGSAPFHWLYHKCGCCGSYNTRVIKVDQISDFLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GY82</accession>
    <name>A0AAD5GY82_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_005611</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000356">
        <property type="protein sequence ID" value="KAI7756521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GY82"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051564">
        <property type="entry name" value="LRR_receptor-like_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48055">
        <property type="entry name" value="LEUCINE-RICH REPEAT RECEPTOR PROTEIN KINASE EMS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48055:SF55">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7756521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="172" mass="19119" checksum="174D1A4B5FE9C453" modified="2024-05-29" version="1">MVAHVGDFGLARFLGTDVNKNNSLAIKGTIGYAAPEYGLGSEMTSSGDVYSFGILLLEVMTGKRPTDNIFNDGLNLHQFAYKALQDNHVTEVIDGDLLHHHQDVENDSDLNYDEDDEIAMKSKAHDTEKIEECMTSILKIGVLCSLDSPPQRMDIKNVVHELQRTIDTLRNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GZV5</accession>
    <name>A0AAD5GZV5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_001468</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000112">
        <property type="protein sequence ID" value="KAI7757483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GZV5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041896911" evidence="2">
        <location>
            <begin position="16"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="12"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7757483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="100" mass="10973" checksum="D7BBB03CDCC347F5" modified="2024-05-29" version="1">MLLFVLICFTQPGGNDDSYQVGATERRNPAYRPDMHGKSRPPYDLNDNNSHGNQISSAPKAPTSFSMGGTFKVKPDDDGVDRKASSSGWMRSLHRISSAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5H0J1</accession>
    <name>A0AAD5H0J1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Aminotransferase class I/classII large domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_018740</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. BioF subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000016">
        <property type="protein sequence ID" value="KAI7758218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5H0J1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009102">
        <property type="term" value="P:biotin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006665">
        <property type="term" value="P:sphingolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001917">
        <property type="entry name" value="Aminotrans_II_pyridoxalP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050087">
        <property type="entry name" value="AON_synthase_class-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13693:SF77">
        <property type="entry name" value="8-AMINO-7-OXONONANOATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13693">
        <property type="entry name" value="CLASS II AMINOTRANSFERASE/8-AMINO-7-OXONONANOATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00599">
        <property type="entry name" value="AA_TRANSFER_CLASS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="339"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="11">
        <location>
            <begin position="98"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7758218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="469" mass="51793" checksum="B837CD8BDFC825B6" modified="2024-05-29" version="1">MSCWDEWVEEALSRLESMKILRSLRPIHYPLENKPKTDNALLVFDEMRQWDRASVEIQIAESTFQRWLKDVPSSGDDLTCEDTDSECELGLCPERFRKLLLFSGNDYLGLSSHPAVANAATKVVQDHGMGPRGSALICGYTTYHRQLESCLAESKNKEDCLLCPTGFSANMAFMTVAGYVGSLSAVNETDCQDGVAIFSDALNHASIIDGIRLAERQGCVQVFIYRHCDMNHLNGLLLNCSMKKKVVVTDSLFSMDGDYAPMVQLSKLRKKHGFLLVVDDAHGTLVCGKNGGGVPEEFGCANDVDICIGTLSKAVGCHGGFIACSKRWKQLIQSRGRAFIFSTSTPVPIIAAAHAAVVVAKKETWRRKAIWNRVQEFRTLTGIPITSHIISLVVGSEEQALKASRHMLKCGFHITAIRPPTVPPNLCRLRITLSAAHTKSDIRKLTFALSQCISLRESGYFYTNGTPKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BJS8</accession>
    <name>A0AAD5BJS8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Mitochondrial import inner membrane translocase subunit TIM50</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_017285</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the TIM23 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TIM50 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012222">
        <property type="protein sequence ID" value="KAI7724442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BJS8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005744">
        <property type="term" value="C:TIM23 mitochondrial import inner membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050365">
        <property type="entry name" value="TIM50"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12210">
        <property type="entry name" value="DULLARD PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="1" id="KW-0653">Protein transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="1" id="KW-0811">Translocation</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="FCP1 homology" evidence="3">
        <location>
            <begin position="94"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="240"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="240"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU365079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7724442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="259" mass="29284" checksum="981C82F60C849733" modified="2024-05-29" version="1">MSMVTRSRQFFSIVKRRNKNPRLYSSVSANGHSEPVISSSILGNQPTSSIPPPPLESAAVDSGVGGKPWSFLKYTLVAALTGGVATAGYATYAYTLEEVDEKTKALRASAKFSGGIDLSSFDRDRGWRTFKRPGVDDFLEHLAQFYEIIVYSDQQSMYVDPVVDRDPSRVLYVSGNALESCLQSENCVPIKPWKCETEDTTLLDLIPFLEYVARHRPADIRPVLASYKGHDIAKEFIERSKEHQRRRQEQKQQGRLWRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BMB5</accession>
    <name>A0AAD5BMB5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Alpha-galactosidase</fullName>
            <ecNumber evidence="4">3.2.1.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Melibiase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_007005</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.</text>
            <dbReference type="EC" id="3.2.1.22"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 27 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.22" evidence="4"/>
    <dbReference type="EMBL" id="JAMZMK010012084">
        <property type="protein sequence ID" value="KAI7725046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BMB5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009505">
        <property type="term" value="C:plant-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004557">
        <property type="term" value="F:alpha-galactosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14792">
        <property type="entry name" value="GH27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002241">
        <property type="entry name" value="Glyco_hydro_27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026960">
        <property type="entry name" value="RVT-Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11452:SF80">
        <property type="entry name" value="ALPHA-GALACTOSIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11452">
        <property type="entry name" value="ALPHA-GALACTOSIDASE/ALPHA-N-ACETYLGALACTOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16499">
        <property type="entry name" value="Melibiase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13966">
        <property type="entry name" value="zf-RVT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00740">
        <property type="entry name" value="GLHYDRLASE27"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase zinc-binding" evidence="5">
        <location>
            <begin position="103"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7725046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="395" mass="43904" checksum="BC6AFDD204CC7314" modified="2024-05-29" version="1">MGFLAEEGDVGNGEQTRFWLDAWLGDTDLKSRFPLLFNRSKDKSAMVKDVWKEGGWFPIDFNEMFSQAEAQEWSALSPLLAQTRLVCKADKWLWDGSNSFAVSDVRKKIKDYRDAQVNFKFKWVSWVPTKCSVFAWRADLGRVPTVDALSKRGVHFDQATCNLCGAENESANHIFADYGFSSGVWTGIGWWVVLLVTRMVVGGGGYGVVGPGPAVGGIVDRIMLPERRNLLASGLADTPPMGWNIWKHFSCNINETVIRETADALVSTGLADLGYKYVNIDVCWAEKTHNQKGNLVARKSTFPSGIKALDDYVHNKGLKLGVYADSGTMTCSKMMPGSLGHKEQDAQTYPIMTRALMDTGRPIFFSLCEWGDLHPALWGSKVGNSWRTTNDISDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BMT1</accession>
    <name>A0AAD5BMT1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_008606</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011726">
        <property type="protein sequence ID" value="KAI7726262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BMT1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37250">
        <property type="entry name" value="OS05G0496000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37250:SF1">
        <property type="entry name" value="OS05G0496000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7726262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="113" mass="12490" checksum="73E3D6E3666D1E50" modified="2024-05-29" version="1">MEFNSANIPHGFTPDVSSTKNDTVKQETVKENKVEDEHDMGSGDVNMEASVTSDDVMRAGGYGARDDISSFLPVASDSTDFEASLRDAREYEEPQKDVCRPGLGWKEDVKKQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BNM5</accession>
    <name>A0AAD5BNM5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Desiccation-related protein PCC13-62</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_010221</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011930">
        <property type="protein sequence ID" value="KAI7725494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BNM5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052965">
        <property type="entry name" value="Pigment-catalase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31694">
        <property type="entry name" value="DESICCATION-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31694:SF12">
        <property type="entry name" value="DESICCATION-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13668">
        <property type="entry name" value="Ferritin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041915662" description="Desiccation-related protein PCC13-62" evidence="1">
        <location>
            <begin position="19"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7725494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="292" mass="31656" checksum="CDF7ADF20EC1F0B5" modified="2024-05-29" version="1">MVTTCMSMSILLLASAKAKLPKSDMDLLEFPLNLEYLEAEFFLYGSMGRGLDHIQPNLTGGGPPPVGVRTANLSPLVRDIIAQFGYQEIGHIRAIKSTVPGFARPLMNLSAASFANVINNAFGRPLHPPFDPYANDINYLIASYVIPYVGLTGYVGATPLLQTPTAKRLVGGLLGIESGQDAVLRTLLYQRAKERLIPYGVTVAEITNRISELRNKLGDAGLKDEGLINIDVGGEITGNILAANDTYWLSYGRTPEEVLRIVYQTGKEQHPGGFFPCGANGTIARQYLKSAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BPM8</accession>
    <name>A0AAD5BPM8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_033375</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011407">
        <property type="protein sequence ID" value="KAI7727247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BPM8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957:SF569">
        <property type="entry name" value="RAB-GTPASE-TBC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="1">
        <location>
            <begin position="67"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7727247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="212" mass="24115" checksum="A268FB71E9A6C03A" modified="2024-05-29" version="1">MMNICRKDPGLPTDSFYEIRAGCTDVPVTKFRIKAGKTLSVRRWRSAFTPEGYLDISKCLSRIYSGGIHPSIRGEVWEFLLGCYDPKSTFDEREKIRQRRSHMFPVVGSGKFVTARVITANGDPVQDPVVLLEANLENLPTPESQESDAVDSREHDKDIIQWKLALHQIGLDVVRTDRSLTFYEKQENLAKLWDVLAVYAWYDKDVGYGQGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BQG1</accession>
    <name>A0AAD5BQG1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein RRC1-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_019324</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011345">
        <property type="protein sequence ID" value="KAI7727575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BQG1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd21371">
        <property type="entry name" value="cwf21_RRC1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12223">
        <property type="entry name" value="RRM_SR140"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.790">
        <property type="entry name" value="Surp module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006569">
        <property type="entry name" value="CID_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013170">
        <property type="entry name" value="mRNA_splic_Cwf21_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047491">
        <property type="entry name" value="RRC1-like_cwf21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051485">
        <property type="entry name" value="SR-CTD_assoc_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035009">
        <property type="entry name" value="SR140_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000061">
        <property type="entry name" value="Surp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035967">
        <property type="entry name" value="SWAP/Surp_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23140">
        <property type="entry name" value="RNA PROCESSING PROTEIN LD23810P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23140:SF0">
        <property type="entry name" value="U2 SNRNP-ASSOCIATED SURP MOTIF-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04818">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08312">
        <property type="entry name" value="cwf21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01805">
        <property type="entry name" value="Surp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01115">
        <property type="entry name" value="cwf21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00582">
        <property type="entry name" value="RPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00648">
        <property type="entry name" value="SWAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109905">
        <property type="entry name" value="Surp module (SWAP domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51391">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50128">
        <property type="entry name" value="SURP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="181"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="SURP motif" evidence="6">
        <location>
            <begin position="330"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="CID" evidence="7">
        <location>
            <begin position="437"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="54"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="671"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="714"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="828"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="15"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="95"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="854"/>
            <end position="927"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7727575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="927" mass="104438" checksum="152D55D942A676F5" modified="2024-05-29" version="1">MSSFSITRKKTPFQKHREEEEAKKKRAEDETARLYAEFLESFQGDTAGLKTFVRGGTVNDEKVKPDTGGNSKEVSGSKKGSRYVPSFIPPPMATKGKEQERQKDEDRPKEKAKGKARNIDFFMEELKHEQEMREKRMQEREQWQDGRGATGNLSSRFDELPDEFDPSGKFGSFDDGDPQTTNLYVGNLAPQVDENFLLRTFGRFGPIASVKIMWPRTEEERKRQKNCGFVAFMNRADGQAAKDEMQGVVVYDYELNIGWGKSVSLPSQSLPAPPPGQMAIRSKEGATVILSGPSGPPVTSVPSQNSELVLTPNVPDINVFLLDDVRLRKVIDTMALYVLDGGCAFEQAIMERVRGNPLFSFLFELGSKEHTYYVWRLYSFAQGDTLQRWRTEPFIMITGSGRWVPPALPIAKSPENEDTGATFAAGRSRRVEAERTLTDAQRDEFEDMLRALTLERTQIKEAMGFALDNADAAGEIVEVLTESLTLKETPIPTKVSRLMLVSDILHNSSAPVKNASAYRTKFEANLPDILESFNDLYRSVTGRITAEALKERVLKVLQVWADWFLFSDAYVNGLRSTFLRSGNSGVVSFHSISSETEDKSNYDDTGDVGKTNPDAALAIGKSAAMKELMGLPLAELERRCRHNGLSLVGGREMMVARLLYLEESEKQRGYELNDSLKYPQNTPRESDVDTGPVGFSGRHHHVEDDIKTLSEGRVSASLPSQIPTPQPDLKVFPNKVKSDSVLPASKWAREDDESDDEQKSTKDLALGYSSSGSENAGGDLGNAEVTDHTSILTSSDSGLNEEKRHKLRQIEVALIEYRETLEERGIKKEEIEKKVVAHQKQLQYKYGLIDGSEDGSHKRTSSTDRREKREDSRESSRKRPRSHHDSESPQRKSSSRDREKGRTYEPQSERDHDRDKRRDRDRDSRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BR47</accession>
    <name>A0AAD5BR47_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Zinc transporter 11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_006911</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011607">
        <property type="protein sequence ID" value="KAI7726679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BR47"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003689">
        <property type="entry name" value="ZIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040:SF217">
        <property type="entry name" value="ZINC TRANSPORTER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040">
        <property type="entry name" value="ZINC/IRON TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02535">
        <property type="entry name" value="Zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042032060" description="Zinc transporter 11" evidence="6">
        <location>
            <begin position="23"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="46"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="77"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="115"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="177"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="203"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="238"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="269"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="307"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7726679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="327" mass="35143" checksum="2B2F2189405B88A2" modified="2024-05-29" version="1">MFRHLFFTSLLLLLILSAAAHGGADHDDDDATAGDTEKPNLRSKSLILVKIWCLIIIFFATFIGGVSPYFLKWNDGFLILGTQFAGGVFLGTAMMHFLSDANETFQDLTSVEYPFAFMLACGGYLLTMVADCVIFYVYGKQSGSDVDGDQEDNRNGKDGNAHPSIGITSASSFGDSILLIVALCFHSVFEGIAIGIADSKADAWKALWTISLHKIFAAIAMGIALLRMIPDRPLLSCASYAFAFGISSPIGVAIGIVIDATTQGRVADWIFAISMGIACGVFIYVSINHLLRGHQAHKTAAVDTPHYKLLAVTLGLGVIAVVMIWDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BR99</accession>
    <name>A0AAD5BR99_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Cellulose synthase</fullName>
            <ecNumber evidence="21">2.4.1.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">M8C21_005952</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="23">AA19_3_7</strain>
            <tissue evidence="23">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17 21">
            <text>[(1-&gt;4)-beta-D-glucosyl](n) + UDP-alpha-D-glucose = [(1-&gt;4)-beta-D-glucosyl](n+1) + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19929"/>
            <dbReference type="Rhea" id="RHEA-COMP:10033"/>
            <dbReference type="Rhea" id="RHEA-COMP:10034"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18246"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.4.1.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="21">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="21">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 21">Glycan metabolism; plant cellulose biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 21">Cell membrane</location>
            <topology evidence="2 21">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 21">Belongs to the glycosyltransferase 2 family. Plant cellulose synthase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.12" evidence="21"/>
    <dbReference type="EMBL" id="JAMZMK010011316">
        <property type="protein sequence ID" value="KAI7727814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BR99"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016760">
        <property type="term" value="F:cellulose synthase (UDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030244">
        <property type="term" value="P:cellulose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000009">
        <property type="entry name" value="Cellulose synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005150">
        <property type="entry name" value="Cellulose_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027934">
        <property type="entry name" value="CES_Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13301">
        <property type="entry name" value="X-BOX TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03552">
        <property type="entry name" value="Cellulose_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14569">
        <property type="entry name" value="zf-UDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 21" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16 21" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="12 21" id="KW-0135">Cellulose biosynthesis</keyword>
    <keyword evidence="6 21" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="15" id="KW-0464">Manganese</keyword>
    <keyword evidence="14 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 21" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 21" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11 21" id="KW-0862">Zinc</keyword>
    <keyword evidence="10 21" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="754"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="787"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="823"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="871"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="903"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="937"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="domain" description="Cellulose synthase RING-type zinc finger" evidence="22">
        <location>
            <begin position="4"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="active site" evidence="18">
        <location>
            <position position="298"/>
        </location>
    </feature>
    <feature type="active site" evidence="18">
        <location>
            <position position="676"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="262"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="268"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="269"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="298"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="439"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="440"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="464"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR605150-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR605150-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR605150-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU361116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF14569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="KAI7727814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="977" mass="109712" checksum="88411539FE47FA85" modified="2024-05-29" version="1">MMHAETPLCNTCGEQIGFASDGNGLFVACHECNFPICKSCLEYEINEGHNACIQCSTPYDVSLTTVGVAEKEPIARNTMAGHLSNSQDAGLHARTVSTVSTVDSDMIDEGGNPIWKNRVESWKDKKNKKKKGGGKVAKEVQVPADQHIEEKQQSADPNAMQPLSQIIPIPKSQITPYRIVIILRLIILGLFFHYRITNPVESSYGLWLTSVICEIWFAISWVLDQFPKWSPINRITFTDELSARYEREGEPSELAAVDFFVSTVDPLKEPPLITANTVLSILAVDYPVEKVSCYVSDDGAAMLTFESLVETSEFARKWVPFCKRFSIEPRAPEFYFSQKIDYLKDKVQPSFVKERRAMKREYEEYKVRVNALVAKAQKTPEEGWTMQDGTPWPGNNPRDHPGMIQVFLGHSGAHDIEGNELPRLVYVSREKRPGYQHHKKAGAENALVRVSAVLTNAPYILNLDCDHYLNNSKAVREAMCFLMDPQVSRDVCYIQFPQRFDGIDRSDRYANRNTVFFDVNMKGLDGIQGPVYVGTGCVFYRQALYGYGPQSLPTLPSPSSSSSCCCGPKKPKKDLEEFKRDARRDDLNAAIFNLKEIESYDDYERSLLISQMSFEKTFGMSSVFIESTLMENGGLAESANPATMINEAIHVISCGYEEKTAWGKEIGWIYGSVTEDILTGFKMHCRGWRSIYCMPVRPAFKGSAPINLSDRLHQVLRWALGSVEIFLSRHCPLWYGWGGGRLKLLQRLAYINTIVYPFTSLPLVAYCTLPAICLLTGKFIIPTLSNLAAVWFLCLFVSIITTSVLEIRWSGVSIEELWRNEQFWVIGGVSAHLFAVFQGFLKMLAGVDTNFTVTAKAADDQEFGELYMIKWTTVLIPPTTLLVLNLVGVVAGFSDALNKGYEAWGPLFGKVFFAFWVILHLYPFLKGLMGRQNRTPTIVVLWSVLLASVFSLVWVKIDPFVSKGDSSLTQGCIAIDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BRV3</accession>
    <name>A0AAD5BRV3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_005781</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011235">
        <property type="protein sequence ID" value="KAI7728325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BRV3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37215">
        <property type="entry name" value="ACYL-COA-BINDING DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37215:SF1">
        <property type="entry name" value="ACYL-COA-BINDING DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="9"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="51"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7728325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="128" mass="15257" checksum="632B8ABC2F79C897" modified="2024-05-29" version="1">MIIIMRRRLWIGVLIVMALGLSIVYLRLWTIDYQISSTETELLRRQFDLANKEAVDESAEWRLRFDEAEERATKCAKELKRVKENIGDNRMTQRLDMLQKENINLLERVETLKQELEAEKLKCSMQKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BRZ7</accession>
    <name>A0AAD5BRZ7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_001101</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011193">
        <property type="protein sequence ID" value="KAI7728583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BRZ7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24089">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 6 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="6">
        <location>
            <begin position="100"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="6">
        <location>
            <begin position="194"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="6">
        <location>
            <begin position="288"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7728583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="384" mass="41171" checksum="4078F7C96F230D8E" modified="2024-05-29" version="1">MDTRGLLESNGDGLFPNSGIGFLWCPRDDNMSQSGAFFASVGQMGRGSFGSVNDNEGVKLPCLSKVVSTPESGFMVLEVPKVGNFVKSKSGLKLKIKIGNPSLRRLISGAIAGAVSRTCVAPLETIRTHLMVGSSGHSTTEVFQDIMQTEGWTGLFRGNLVNVIRVAPSKAIELFAYDTVKKSLTPKLGEKSKPRIPESLIAGAAAGISSTICTYPLELLKTRLTVQRGVYNNLVDAFLKIVKEEGPAELYRGLTPSLIGVVPYAATNYFAYDTLRKVYKKILKKDEVGNIATLLIGSAAGAISSSATFPLEVARKHMQAGAVNGRVYDNMLHALLTILEKEGVVGLYRGLGPSCIKIVPAAGISFMCYEACKKILVETEDDEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BTC1</accession>
    <name>A0AAD5BTC1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]</fullName>
            <ecNumber evidence="9">1.3.1.88</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">M8C21_018406</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">AA19_3_7</strain>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>5,6-dihydrouridine(16) in tRNA + NAD(+) = uridine(16) in tRNA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53380"/>
            <dbReference type="Rhea" id="RHEA-COMP:13543"/>
            <dbReference type="Rhea" id="RHEA-COMP:13544"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
            <dbReference type="EC" id="1.3.1.88"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="12">
            <dbReference type="Rhea" id="RHEA:53382"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>5,6-dihydrouridine(16) in tRNA + NADP(+) = uridine(16) in tRNA + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53376"/>
            <dbReference type="Rhea" id="RHEA-COMP:13543"/>
            <dbReference type="Rhea" id="RHEA-COMP:13544"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
            <dbReference type="EC" id="1.3.1.88"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="11">
            <dbReference type="Rhea" id="RHEA:53378"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>5,6-dihydrouridine(17) in tRNA + NAD(+) = uridine(17) in tRNA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53372"/>
            <dbReference type="Rhea" id="RHEA-COMP:13541"/>
            <dbReference type="Rhea" id="RHEA-COMP:13542"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
            <dbReference type="EC" id="1.3.1.88"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="10">
            <dbReference type="Rhea" id="RHEA:53374"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>5,6-dihydrouridine(17) in tRNA + NADP(+) = uridine(17) in tRNA + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53368"/>
            <dbReference type="Rhea" id="RHEA-COMP:13541"/>
            <dbReference type="Rhea" id="RHEA-COMP:13542"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
            <dbReference type="EC" id="1.3.1.88"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="13">
            <dbReference type="Rhea" id="RHEA:53370"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the Dus family. Dus1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.88" evidence="9"/>
    <dbReference type="EMBL" id="JAMZMK010011077">
        <property type="protein sequence ID" value="KAI7729082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BTC1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017150">
        <property type="term" value="F:tRNA dihydrouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02801">
        <property type="entry name" value="DUS_like_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000162">
        <property type="entry name" value="tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035587">
        <property type="entry name" value="DUS-like_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018517">
        <property type="entry name" value="tRNA_hU_synthase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11082">
        <property type="entry name" value="TRNA-DIHYDROURIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11082:SF5">
        <property type="entry name" value="TRNA-DIHYDROURIDINE(16_17) SYNTHASE [NAD(P)(+)]-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01207">
        <property type="entry name" value="Dus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01136">
        <property type="entry name" value="UPF0034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0288">FMN</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="DUS-like FMN-binding" evidence="15">
        <location>
            <begin position="61"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="16"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF01207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAI7729082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="378" mass="43192" checksum="FB8A408F1CE3AB39" modified="2024-05-29" version="1">MAIDSITQTLPPPDSPTNNPQHLCSETTSPLLTPNPYLTFDARIEQAWTHWKSLGEPKLIVAPMVDNSELPFRLLCRKYGAEGAYTPMLHSRIFTENEKYRSTEFTTCKEDRPLFVQFCANDPDTLLEAARRVEPYCDYVDINLGCPQRIAKRGNYGAFLMDKLPLVKSLVEKLAQNLNVPVSCKIRIFPDLQDTLNYAKMLEDAGCALLAVHGRTRDEKDGKKIRANWEAIRAVKNALRIPVLANGNIRHMDDVKRCLEETGVDGVLSAETLLENPALFAGFRTVEWVSDNEEGVQDGKLDQADLVVEYLKLCEKYPVPWRMIRAHLHKMLGDWFRVHPHVRDDFNAQNILSFEFLYDMVNRLRELGVSRPLYVKNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BTI6</accession>
    <name>A0AAD5BTI6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Tubulin/FtsZ 2-layer sandwich domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_018508</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tubulin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011014">
        <property type="protein sequence ID" value="KAI7729361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BTI6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.600:FF:000005">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.20:FF:000001">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.600">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002452">
        <property type="entry name" value="Alpha_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023123">
        <property type="entry name" value="Tubulin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01162">
        <property type="entry name" value="ALPHATUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="11">
        <location>
            <begin position="3"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="174"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7729361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="193" mass="21634" checksum="8DACB08E7C6806F1" modified="2024-05-29" version="1">MFIPYPRIHFMLSSYAPAISAEKASHEQFSVAEITNTAFEPSSMMAKCDQRHGKYMACCLMYRGDVVTKDVNAAVATIKTKRTIQFVDRCPNGFKCGINYQPPTMIPGGDLAKVKSVVCMISNSTSVAEVFSRIDHKFDLMYAKRAYVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGAESGENEDEDEGDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BTS7</accession>
    <name>A0AAD5BTS7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Short-chain dehydrogenase TIC 32, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_030623</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011274">
        <property type="protein sequence ID" value="KAI7728074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BTS7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055280">
        <property type="entry name" value="TIC32"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48476">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE TIC 32, CHLOROPLASTIC-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48476:SF1">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE TIC 32, CHLOROPLASTIC-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7728074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="257" mass="28766" checksum="6A6F9CB6CF8918B0" modified="2024-05-29" version="1">MAVRNREKAIHVKENILQDTPDAKVDVMELDLSSMASVRDFAAKYHSSGFPLNILVNNAGVLSPQFKLSKENIELHFATNHLGHFLLTNLLLETMKTTSFEQKTEGRIINVSSEGHRFAYSGPLSDYTNNESSYNSVYAYGLSKLANILHANELARLLKEDGVNITANSLHPGFIDTELFRGHNIFSVICNKILRYFIKDVSQGASTTCYLALNSQVRGITGEFFSDSNLAKPSMRAKDLELAKELWDFSMCFTSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BU85</accession>
    <name>A0AAD5BU85_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_001771</name>
        <name evidence="6" type="ORF">M8C21_020942</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010930">
        <property type="protein sequence ID" value="KAI7729737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAMZMK010010929">
        <property type="protein sequence ID" value="KAI7729741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BU85"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048262">
        <property type="term" value="P:determination of dorsal/ventral asymmetry"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009908">
        <property type="term" value="P:flower development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000154">
        <property type="entry name" value="Transcription factor SRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044636">
        <property type="entry name" value="RADIALIS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43952">
        <property type="entry name" value="MYB FAMILY TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43952:SF72">
        <property type="entry name" value="MYB-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Myb-like" evidence="5">
        <location>
            <begin position="4"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7729737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7729741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="81" mass="9266" checksum="C357EF853AA2F788" modified="2024-05-29" version="1">MASSSNVWTWEQNKLFENSLATYNNDPDRFQKIAKIIGKTVDEVKVHYQNLVHDVNAIEAGKIPLPNYKTGEAQGQYNKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BUU8</accession>
    <name>A0AAD5BUU8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Pyruvate kinase</fullName>
            <ecNumber evidence="18">2.7.1.40</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">M8C21_009672</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">AA19_3_7</strain>
            <tissue evidence="21">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17 18">
            <text>pyruvate + ATP = phosphoenolpyruvate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18157"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.40"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 18">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 18">Belongs to the pyruvate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.40" evidence="18"/>
    <dbReference type="EMBL" id="JAMZMK010011168">
        <property type="protein sequence ID" value="KAI7728674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BUU8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030955">
        <property type="term" value="F:potassium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004743">
        <property type="term" value="F:pyruvate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00288">
        <property type="entry name" value="Pyruvate_Kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.33.10:FF:000001">
        <property type="entry name" value="Pyruvate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.60:FF:000001">
        <property type="entry name" value="Pyruvate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1380.20:FF:000005">
        <property type="entry name" value="Pyruvate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="entry name" value="Phosphoenolpyruvate-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.33.10">
        <property type="entry name" value="PK beta-barrel domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1380.20">
        <property type="entry name" value="Pyruvate kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001697">
        <property type="entry name" value="Pyr_Knase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011037">
        <property type="entry name" value="Pyrv_Knase-like_insert_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018209">
        <property type="entry name" value="Pyrv_Knase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015793">
        <property type="entry name" value="Pyrv_Knase_brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015795">
        <property type="entry name" value="Pyrv_Knase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036918">
        <property type="entry name" value="Pyrv_Knase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015806">
        <property type="entry name" value="Pyrv_Knase_insert_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004491">
        <property type="entry name" value="PRK05826.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004978">
        <property type="entry name" value="PRK06354.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01064">
        <property type="entry name" value="pyruv_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11817">
        <property type="entry name" value="PYRUVATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00224">
        <property type="entry name" value="PK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02887">
        <property type="entry name" value="PK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01050">
        <property type="entry name" value="PYRUVTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50800">
        <property type="entry name" value="PK beta-barrel domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52935">
        <property type="entry name" value="PK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00110">
        <property type="entry name" value="PYRUVATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15 18" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="11 18" id="KW-0418">Kinase</keyword>
    <keyword evidence="13 18" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0630">Potassium</keyword>
    <keyword evidence="16" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 18" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyruvate kinase barrel" evidence="19">
        <location>
            <begin position="19"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate kinase C-terminal" evidence="20">
        <location>
            <begin position="378"/>
            <end position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF02887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAI7728674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="508" mass="55197" checksum="F24A7E206929FA53" modified="2024-05-29" version="1">MDQMKTDQSMQQIGVKRPKTKIVCTLGPASRSVEMVEKLLKAGMNVARFNFSHGSYEYHQETLDNLNTAMDNTGILCAVMLDTKGPEIRTGFLKDGKPIQLKQGQEITISTDYDIKGDETMICMSYKKLAYDVKPGMVILCADGTISFTVLACDTENGLVRCRCENTAVLGERKNVNLPGVIVDLPTLTEKDKEDILRWGVPNKIDMIALSFVRKGSDLVEVRKLLGEHAKNILLMSKVENQEGVANFDEILANSDAFMVARGDLGMEIPIEKIFLAQKVMVYKCNIQGKPVVTATQMLESMIKSPRPTRAEATDVANAVLDGTDCVMLSGETAAGAYPELAVLTMARICVEAENTIDYPDVFKRIAANAPIPMSPLESLASAAVRTANSSRASLILVLTRGGSTAKLVAKYRPGMPVLSVVVPEIETDSFDWSCSDETPARHSLIFRGLVPILSAGSAKASHAESTEEVLDFALQHAKLKGLCKEGDAVVALHRVGIASVIKIINVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BWW4</accession>
    <name>A0AAD5BWW4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CRAL-TRIO domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_026597</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010674">
        <property type="protein sequence ID" value="KAI7730709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BWW4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47104">
        <property type="entry name" value="SEC14P-LIKE PHOSPHATIDYLINOSITOL TRANSFER FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47104:SF1">
        <property type="entry name" value="SEC14P-LIKE PHOSPHATIDYLINOSITOL TRANSFER FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46938">
        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="1">
        <location>
            <begin position="98"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7730709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="421" mass="47664" checksum="848D4EE502A3D81C" modified="2024-05-29" version="1">MGNKDNSSSTSAQSQMQIQKNKVDAVLQLLRKPSPLTLKQEKFCNEACVARFLKAKGDNVKKTAKQLRATLSWRENLGVDHLIADEFSGEIADGMAYVAGHDDRSRPVVIFRIKQDYLKFRSQKQFTRLLVFTLEVAIQTMAKSVEQLIVLFDASFFRSASGFMNILVAALKTIGEHYPGRLHKAFVIDPPSLFPYLWKGVKAFIELSSITLMVSSLDFDDFPDFNNFTPYPRAASLRFNPSSVPTKGAKVGSCSSSRFSFTVAHHFDSLKPWYLTLTDKPSFKVGPTSTPVLGPALISPVNARSYSFASPTARNMNTLRKNFFPSTPLPQKTQVMDHSGINHPRTPKPSFLHSPALFFKKECHLSKTEKCRESFIPFLRFYRRPYDEMIYRSKMKPPLGGLISIVSPQIRRRHMSVSQRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BXB2</accession>
    <name>A0AAD5BXB2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">PNPLA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_011351</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010541">
        <property type="protein sequence ID" value="KAI7731115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BXB2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07231">
        <property type="entry name" value="Pat_SDP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050301">
        <property type="entry name" value="NTE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021771">
        <property type="entry name" value="Triacylglycerol_lipase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14226:SF77">
        <property type="entry name" value="ACYL TRANSFERASE_ACYL HYDROLASE_LYSOPHOSPHOLIPASE, TRIACYLGLYCEROL LIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14226">
        <property type="entry name" value="NEUROPATHY TARGET ESTERASE/SWISS CHEESE D.MELANOGASTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11815">
        <property type="entry name" value="DUF3336"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 4" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="3 4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041938875" description="PNPLA domain-containing protein" evidence="6">
        <location>
            <begin position="27"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="domain" description="PNPLA" evidence="7">
        <location>
            <begin position="173"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="727"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="4">
        <location>
            <begin position="204"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="749"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="206"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7731115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="777" mass="87601" checksum="23BF9738847ECED4" modified="2024-05-29" version="1">MSWLHPRNPQGILVLVMLMAFLLKRCTNVKMRAEMAYKRKFWRNMMRAAVTYEEWAHAAKMLDKETPRMNECNLYDEELVRNKLQELRHRREEGCLRDVIFYMRADLVRNLGNMCNPELHKGRHQVPKLIKEYIDEVSTQLRMVCDSDCEELLLEERLAFMHETRHAFGRTALLLSGGASLGAFHVGVVKTLVEHKLLPRIIAGSSVGSIICAVIATKSWPELQSFFEESFDSFQFFGQLGGVFSIFKRVISLGALHDIRCLQVLLRNLTNNLTFQEAYDMTGRVLGITVCSPRKHEPPRCLNYLTSPHVVIWSAVTASCAFPGLFEAQELMAKDRSGELVPYHPPFHWGPEEANGSSTRRWRDGSLEIDLPMMQLKELFNVNHFIVSQANPHISPLLRIKEVVRAYGGRFAAKLAHLVEMEVKYRCNQVLELGFPLGGLAKLFAQDWEGDVTIVMPATLAQYSKIIRNPSHLEIQKAANQGRKRTWEKLSAIKANCGIELVLDECVAILNHMRRLKRSAERAASVSATATHGLANTARFNASKRIPSWNCMARENSTGSLEDLADMSSSLHQGHGGRNWRFNHSMHDGSDSESETAELHSWTRSGGPLMRTASADQFVDFVQNLDFDSRTTNKAMPHHQNSRSIVIPPEADTSFDLEPDQRDMNNRGVHTSITVTEGDLLQPEMIHNGIVFNVVKRGDLTPSNRSLDSETINSPSAVAECVQLDCPEKETDASSASEDGDADIRNDNHVSTPDYTSNMKDHTIFNNDDDAGDHQPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BY21</accession>
    <name>A0AAD5BY21_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_028828</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010758">
        <property type="protein sequence ID" value="KAI7730506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BY21"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7730506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="203" mass="23097" checksum="5A6A5A7A421945AE" modified="2024-05-29" version="1" fragment="single">EANDRCARTTPTVCRFRRLRRFHGVDGRGSGQVVFVPLTSDIVRKTSEGVEIGQLLLSLTTGQAGSYGRRSITSLKLRHLYAKVLVVSKVPLPNYRFDLDDKRPQREVSLPPELQMRVEAHLGEYLSHKAKNNNGSQDSLFSKTTNNGSICNDEGLFEQPELGPQIQLAMEKVLRIRSVQLQTDQQAWQVQDLFFTLMYLKFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BZX1</accession>
    <name>A0AAD5BZX1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_022171</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TOM1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010140">
        <property type="protein sequence ID" value="KAI7732843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BZX1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14231">
        <property type="entry name" value="GAT_GGA-like_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03561">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004152">
        <property type="entry name" value="GAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038425">
        <property type="entry name" value="GAT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044836">
        <property type="entry name" value="TOL_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002014">
        <property type="entry name" value="VHS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45898:SF4">
        <property type="entry name" value="TARGET OF MYB PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45898">
        <property type="entry name" value="TOM1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03127">
        <property type="entry name" value="GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00790">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00288">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89009">
        <property type="entry name" value="GAT-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50909">
        <property type="entry name" value="GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50179">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="VHS" evidence="7">
        <location>
            <begin position="38"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="GAT" evidence="8">
        <location>
            <begin position="178"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="146"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="350"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="416"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="157"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="359"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="416"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7732843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="631" mass="68898" checksum="65EFC7770E5D2D8E" modified="2024-05-29" version="1">MKDGRSLTVGAQRLKVFQFHFHYYIGSENPEGDSIRRAKDVVKGIKKRMGSKNPKAQLLALTLLETMMKNCGDFVHLHVAERDVLHEMVKIVKKKPDFHVKEKILILIDTWQEAFGGARARYPQYYAAYHELLRLGAVFPQKADRPAPVLTNPQTQSSSSSSYANNPENGNEAAESSEFPTLSLTELQNARGIMDVLAEMLTAIDPEKKEGLRQEVIIDLVEQCRTYKQRVVHLVNSTSDESLLCQGLALNDDLQRVLAKHEALISGTSQKAKPETSSQALVPVEASLIDTGDTKQSPAAVGTSSSTGTGVEADLLAITTVTTNAAAEVNPKFDLLSGDDFSSENSLAIVPMPMGEPESTSTTTTTASQSQQHDALALVDILSQNNNVPSAPVLAGQTAHQFQPSQSLDSLYTNAYTSNTPYDPQTPFNLGSTSSWNGHLSQQQPPSPPAAFGSEGLPPPPWEAQSMDDNKLGSDQGMSMGMGMYNQQPMSATHIPPLNKQFVYPQQQFQGQQIMGGMGMIPQHQIQPGQVHMYPPQQLAQQMYANQMPQPPPPPYGYGYGYGYAQQPNTQQFIDQRMSGLSLNNNATTVYKGPSMANASYVHVPSGKPTKAEDKLFGDLVDFSKVKPNKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C1G1</accession>
    <name>A0AAD5C1G1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_007240</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010049">
        <property type="protein sequence ID" value="KAI7733265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C1G1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7733265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="115" mass="13066" checksum="0F0D004BC136734F" modified="2024-05-29" version="1">MVKKGEQFCRSAICKISQARVGTFDEYVLGWVMSTTFARRTVSKKGDIGQLFIAFDGVVVEPINLTVPVTQAIATTFTSWLVDKETTDSSKTSQNIYGRFLHKSYSFSWNCWCRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C354</accession>
    <name>A0AAD5C354_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_001278</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009815">
        <property type="protein sequence ID" value="KAI7734145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C354"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033612">
        <property type="term" value="F:receptor serine/threonine kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000896">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000221">
        <property type="entry name" value="Leucine-rich repeat receptor-like protein kinase PXL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000095">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050647">
        <property type="entry name" value="Plant_LRR-RLKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48056">
        <property type="entry name" value="LRR RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48056:SF25">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="498"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="505"/>
            <end position="805"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7734145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="805" mass="88510" checksum="27BFEC1C3CE9A1A4" modified="2024-05-29" version="1">MFTVSSATDPFTEALLSLKSEFTTPPNVLKDWVITPKDPNPSQKIQSCTWTVKVINFAGSYFSGPIPGSYGLCKNLDFIHLAGNMLTGYLPVEFGQLKTVTHMEIGYNFYKGSIPWEFSNMSELRYLDIAGANLSGLIPKELGRLVKLDTLFLFKNSLSGTIPDEFGNISTLSSLDLSDNFLSGPIPDSFAKLKSLKLLSVMFNDMNGSVPDGIAKLPNLESLFIWDNLFSGTLPQELGMHSKLRWVDVSTNDFVGIIPSGICSGGELSKLMLFSNYFSGGLSSIANCSSLVRIRLEDNSFSGDVSLDFKVLSDVSYVDVSGNRLTGGIPYDIFHALKLEHFSVSNNPNLGGILPEKVWSLPVLQNFSASSCNVSGNFPEFPFCKSLSFIDLHGNHLSGNIPESISLCENLETLNFAENNLSGEIPVKLGSLMKLKFLNLSYNDLSGLIPNGNTFKSMDSSSFLGNPNLCGAPLARVCHHGNGISEGMELGSRRNHKVAWVLVLCAVVVVLSSLLFGIFYYRRQSVNRHWKMVSFGGLPELTAADVLKSFNSIDSVETLYSSNSVCKAVLLSGMTVIVRKIEWGAKSSNLLVDFINQIGNLRHKNLIRLLGFCYNKNLGYLLYEYLPNGNLDDKIGIKRDWGSKHRLVINIAKGLCYLHHDCHPAIPHGDLKASNIVFDENMEPRLAEFGFKTISAMETGEYKGLMDHDLKDDVFDFGEVVLEILTNGRRKNGGISIHKAPKDVLLKEIYNDNDVSASSSSSSSSKSIQEEIKTVLEVVLSCTTSKQSDRPSMEDVLKILSGLKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C3P5</accession>
    <name>A0AAD5C3P5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Superoxide dismutase [Cu-Zn], chloroplastic</fullName>
            <ecNumber evidence="6">1.15.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">M8C21_028772</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">AA19_3_7</strain>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Destroys radicals which are normally produced within the cells and which are toxic to biological systems.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 18">
            <text>2 superoxide + 2 H(+) = H2O2 + O2</text>
            <dbReference type="Rhea" id="RHEA:20696"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:18421"/>
            <dbReference type="EC" id="1.15.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the Cu-Zn superoxide dismutase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.15.1.1" evidence="6"/>
    <dbReference type="EMBL" id="JAMZMK010009963">
        <property type="protein sequence ID" value="KAI7733504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C3P5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004784">
        <property type="term" value="F:superoxide dismutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00305">
        <property type="entry name" value="Cu-Zn_Superoxide_Dismutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.200:FF:000003">
        <property type="entry name" value="Superoxide dismutase [Cu-Zn], chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.200">
        <property type="entry name" value="Superoxide dismutase, copper/zinc binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036423">
        <property type="entry name" value="SOD-like_Cu/Zn_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024134">
        <property type="entry name" value="SOD_Cu/Zn_/chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018152">
        <property type="entry name" value="SOD_Cu/Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001424">
        <property type="entry name" value="SOD_Cu_Zn_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10003">
        <property type="entry name" value="SUPEROXIDE DISMUTASE CU-ZN -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00080">
        <property type="entry name" value="Sod_Cu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00068">
        <property type="entry name" value="CUZNDISMTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49329">
        <property type="entry name" value="Cu,Zn superoxide dismutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00087">
        <property type="entry name" value="SOD_CU_ZN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="11" id="KW-0186">Copper</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Superoxide dismutase copper/zinc binding" evidence="15">
        <location>
            <begin position="81"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00067111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAI7733504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="215" mass="21982" checksum="8252722C4C232632" modified="2024-05-29" version="1">MAAHSILFSSTTTITNTLILPLSNPNNNINLSLHSSFHGVSLKPAVNRQTLTLSAATAPKPLTVVAATKKAVAVLKGTSSVEGVVTLTQEDTGPTTVNVKISGLTPGPHGFHLHEFGDTTNGCISTGPHFNPNGHTHGAPEDEIRHAGDLGNIIANADGVAEATIVDNQIPLTGPNAVVGRAFVVHELADDLGKGGHELSLSTGNAGGRLACAYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C6H4</accession>
    <name>A0AAD5C6H4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein root UVB sensitive 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_021704</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RUS1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009624">
        <property type="protein sequence ID" value="KAI7734901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C6H4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006968">
        <property type="entry name" value="RUS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055412">
        <property type="entry name" value="UVB_sens_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054549">
        <property type="entry name" value="UVB_sens_RUS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12770:SF20">
        <property type="entry name" value="PROTEIN ROOT UVB SENSITIVE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12770">
        <property type="entry name" value="RUS1 FAMILY PROTEIN C16ORF58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24160">
        <property type="entry name" value="UVB_sens_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04884">
        <property type="entry name" value="UVB_sens_prot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein root UVB sensitive/RUS" evidence="2">
        <location>
            <begin position="2"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="Root UVB sensitive protein C-terminal" evidence="3">
        <location>
            <begin position="231"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7734901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="393" mass="43229" checksum="72B04DA5D8876F5C" modified="2024-05-29" version="1">MSFIRSYVVPEGFPDSVSPSYVPYMTWRALKHFFGGAMGVFTTQALLHSVGVSKSQAMPGAVAINWIIKDGSGRIGKMLFSRQGKKFDYDLKQLRLSGDLLMELGAAVELATTAVPHLFLPLACAANVAKNVAAVTSTSTRTPIYKAFAKGENIGDVTAKGECVGNVADLLGTGFSIIMAKRNPSLFTTFALLSCGYLFSSYREVKSVVLHTLNRARFTVAVESFLKTGSVPTLQEGNMMETVFNIPWCKDGPVVLGSRFKEAFQDANSFYDVKPVFEKERYVVAYNPLKGNIYAVLKDKAKSDDILKAAFHAHVLLYVLRSAKHIQVSNEYTRGDRVTPLPTSVDLQAHVAESYNMVSALYEPFKSKAKEQGWVMSDSLLNPGRARICEQVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C6L3</accession>
    <name>A0AAD5C6L3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_022372</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009398">
        <property type="protein sequence ID" value="KAI7735960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C6L3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7735960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="133" mass="14969" checksum="F340F018DC0F44A8" modified="2024-05-29" version="1" fragment="single">MSLITIAGGDFSNHLTPSTHHDCCNHRHPHHDCCNHHHSYRHCCNVKLLASFWWGKNFTANGVIEEIEVTKEWLPNITRLSKQLVADGSAHAMARTVDQTAKKGEKEGKRQLPLRTFFSIAPTTACFFGYLAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C6W7</accession>
    <name>A0AAD5C6W7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_024120</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the glycosyl hydrolase 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009330">
        <property type="protein sequence ID" value="KAI7736207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C6W7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008422">
        <property type="term" value="F:beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001360">
        <property type="entry name" value="Glyco_hydro_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353:SF246">
        <property type="entry name" value="3-ALPHA-(S)-STRICTOSIDINE BETA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353">
        <property type="entry name" value="GLYCOSYL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00232">
        <property type="entry name" value="Glyco_hydro_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00131">
        <property type="entry name" value="GLHYDRLASE1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7736207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="389" mass="44941" checksum="BF2122F446D0CC24" modified="2024-05-29" version="1" fragment="single">GKVSMGKNLEGISYYNKLIDELKANDIEPFVTLFHWDLPNALEEEYKGFLSSKIVDDFVNYAEICFWEFGDRVKNWVTLNEPYRFAYGGYVEGIFAPGRGGNNQDGDPETEPYIVSYNLLNCHAAAYRKYQEDFKDVQKGQVGITLDTNFFKPFRGKDNKDDVQAVEYAYDFMLGWYLEPITSGNWPVNVQNFAIAPSLHHPQGRYLPVFTDEQKNKLIQSYDFLGINYYTANYAQLYNYSSSLSSGFLDDCHYKASGDKRSKRTDPNGNYIGEPTLFFNSIRLLSSENSIMTKPLVMGYFAWSFTDSFEWNSGYTLRFGMNYVDFKNNLLRYPKKSAIWFKKFLNANTLFETRKRSLIDVEQEDEVNGAQIKAEQAVEVVQKLKKAKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C979</accession>
    <name>A0AAD5C979_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phytocyanin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_019206</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009159">
        <property type="protein sequence ID" value="KAI7736943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C979"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039391">
        <property type="entry name" value="Phytocyanin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003245">
        <property type="entry name" value="Phytocyanin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33021">
        <property type="entry name" value="BLUE COPPER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33021:SF231">
        <property type="entry name" value="EARLY NODULIN-LIKE PROTEIN 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02298">
        <property type="entry name" value="Cu_bind_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51485">
        <property type="entry name" value="PHYTOCYANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042248621" description="Phytocyanin domain-containing protein" evidence="1">
        <location>
            <begin position="30"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="Phytocyanin" evidence="2">
        <location>
            <begin position="30"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7736943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="149" mass="16739" checksum="249FEB1C56DE0E92" modified="2024-05-29" version="1" fragment="single">MGRTNSIWATAIAIAFFATLAIMVPQVSAKRYIVGGSMGWTTNVNYTLWAANQTFFFGDWLSCLDVLYNLFLFLDCTVFVYDRNQNDVIEVNQTNYETCIAEHPVHNYTTGAGRDVVPLNVTHDRYFISSKGSCYGGMKLHVHLSPLPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CAK7</accession>
    <name>A0AAD5CAK7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">S-adenosyl-l-methionine decarboxylase leader peptide</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_005038</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008870">
        <property type="protein sequence ID" value="KAI7738142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CAK7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012511">
        <property type="entry name" value="AdoMetDC_leader"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35727">
        <property type="entry name" value="BNAA05G33520D PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35727:SF5">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE DECARBOXYLASE LEADER PEPTIDE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08132">
        <property type="entry name" value="AdoMetDC_leader"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7738142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="62" mass="6850" checksum="3CBD427704E0FDE1" modified="2024-05-29" version="1">MIFCWQLGQLNVLMESKGGKKSSSSKSFYEAPLGYSIEDVRPNGGIKKFRSAAYSNCARKPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CCW0</accession>
    <name>A0AAD5CCW0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_014566</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008605">
        <property type="protein sequence ID" value="KAI7739562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CCW0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901259">
        <property type="term" value="P:chloroplast rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050502">
        <property type="entry name" value="Euk_RNA-bind_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025">
        <property type="entry name" value="OS02G0815200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025:SF15">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="46"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="115"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="17"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7739562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="200" mass="22151" checksum="9204181ADB9710B5" modified="2024-05-29" version="1" fragment="single">VSLTSPSKLPHFAPHTSDWSQQVTQQTEQDEIEESEGSYAEPPEDAKIFVGNLPYDIDSEKLADLFSSAGVVEIAEVIYNRDTEQSRGFGFVTMSTVEEAEKAVETYNGYDLSGRLLTVNKAAPRGSQPERRVVANPFKIYVDARVIYDRETGRSRGFGFVTMASETEMNDAIAALDGQSLDGRAIRVNAAEERPKRGFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CDN1</accession>
    <name>A0AAD5CDN1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_019635</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008578">
        <property type="protein sequence ID" value="KAI7739687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CDN1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042178045" evidence="2">
        <location>
            <begin position="23"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7739687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="57" mass="5928" checksum="FAED00F8594FBBA3" modified="2024-05-29" version="1" fragment="single">MAMKLPLFLLASFLLLTTSVTSNEQEAAYVATTPAPTPPQVKAPPPSPPVAKPPTPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CEL0</accession>
    <name>A0AAD5CEL0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein LUTEIN DEFICIENT 5, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_025772</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008531">
        <property type="protein sequence ID" value="KAI7740024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CEL0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010291">
        <property type="term" value="F:beta-carotene 3-hydroxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016123">
        <property type="term" value="P:xanthophyll biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11046">
        <property type="entry name" value="CYP97"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050196">
        <property type="entry name" value="Cytochrome_P450_Monoox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291:SF171">
        <property type="entry name" value="PROTEIN LUTEIN DEFICIENT 5, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0349">Heme</keyword>
    <keyword evidence="3 4" id="KW-0408">Iron</keyword>
    <keyword evidence="3 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="2 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="54"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="56"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="3">
        <location>
            <position position="530"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7740024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="611" mass="68082" checksum="71CD00BADE8523BC" modified="2024-05-29" version="1">MVASLTTLQFPSPYLQSHITPTTKFNLKSPFIPKFNVYGAGVSRSCGIKCSYSNGRKPDSGEGKSEMTKEEKRRAELSARIASGAFTVEKPSLGSLLVSSLAKLGMPSAFLEPLSNLINAGENYPKIPEAKGAISAIRSEAFFIPLYELFLTYGGIFRLTFGPKSFLIVSDPIIAKHILKDNAKAYSKGILAEILEFVMGTGLIPADGEVWRVRRRVIVPALHLKYVAAMIGLFGEATDRLCKKLDAAAYNGEDVEMESLFSRLTLDIIGKSVFNYDFDSLTTDNGIVEAVYTVLREAEDRSVSPIPTWEIPIWKDISPRQKKVNEALKLINTTLDDLIAICKRMVDEEDVQFNEEYMNESDPSILHFLLASGDNVSSKQLRDDLMTMLIAGHETTAAVLTWTFYLLSKEPEIMSKLQNEVDAVLGDRFPTMEDMKKLKYTTRVINESLRLYPQPPVLIRRSIDDDVLDKYPIKRGEDLFISVWNLHRSPTHWVDADKFNPERWPLDGPNPNESNQNFSYLPFGGGPRKCVGDMFASFEAIVAVAMLVRRFNFQMALGAPPVKMTTGATIHTTEGLNMTVTRRMKPPIVPTLETQNLGSSVNIPEGDPVVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CF70</accession>
    <name>A0AAD5CF70_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mitochondrial ATP synthase 6 kDa subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_001016</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008619">
        <property type="protein sequence ID" value="KAI7739474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CF70"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052867">
        <property type="entry name" value="ATP_Synthase_Subunit_6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34565">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34565:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7739474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="59" mass="7004" checksum="E04CD6762BCF46D2" modified="2024-05-29" version="1">MRKFDPWNVFFKREFNRNWPFLVGFAVTGTIITKFSLGLTEEDRKNSVFAQRHKSCFAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CG96</accession>
    <name>A0AAD5CG96_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">mitogen-activated protein kinase kinase</fullName>
            <ecNumber evidence="7">2.7.12.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">M8C21_000822</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">AA19_3_7</strain>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.12.2" evidence="7"/>
    <dbReference type="EMBL" id="JAMZMK010008207">
        <property type="protein sequence ID" value="KAI7741362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CG96"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004708">
        <property type="term" value="F:MAP kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06623">
        <property type="entry name" value="PKc_MAPKK_plant_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000432">
        <property type="entry name" value="mitogen-activated protein kinase kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.50:FF:000012">
        <property type="entry name" value="Mitogen-activated protein kinase kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000527">
        <property type="entry name" value="mitogen-activated protein kinase kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018222">
        <property type="entry name" value="Nuclear_transport_factor_2_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48013:SF9">
        <property type="entry name" value="DUAL SPECIFICITY MITOGEN-ACTIVATED PROTEIN KINASE KINASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48013">
        <property type="entry name" value="DUAL SPECIFICITY MITOGEN-ACTIVATED PROTEIN KINASE KINASE 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50177">
        <property type="entry name" value="NTF2_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 11 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 12" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="83"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="NTF2" evidence="14">
        <location>
            <begin position="339"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00051693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAI7741362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="491" mass="54709" checksum="51E4D2657A30B76B" modified="2024-05-29" version="1">MAGLEELKKKLVPLFDAEKGFSSGSTLDPSDSYMLSDGGTVNLLSRSYGVYNINELGLQKCTSRPVKDVDETEKTYQCASHEMRIFGAIGSGASSVVQRAIHIPTHRIIALKKINIFEKEKRQQLLTEIRTLCEAPCYQGLVEFYGAFYTPDSGQISIALEYMDGGSLADIIKRQRCIPEAVLSLMVKKLLQGLSYLHGVRYLVHRDIKPANLLVNLKGETKITDFGISAGLENSMAMCATFVGTVTYMSPERIKNENYSYPADIWSLGLALFECGTGEFPYTANEGPVNLMLQKDADARPTAEQLLSHPFITKYEDSKVDLAAFVRNIFDPTQRMKDLADMLAVHYYLLFDGCDELWQYTESFYNESSTFSFSGKESIGPNDIFTTLSSIRSTLAGDWPSEKLVHVVEKLQCRAHGEDGVAIRVSGSFIVGNQFLICGDGIQAEDVPDFKDLNIDIPSKRMGTFQEQFIMEAGNIIGRYSIAKQELYIIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CGY6</accession>
    <name>A0AAD5CGY6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GDSL esterase/lipase APG</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_020742</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008366">
        <property type="protein sequence ID" value="KAI7740690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CGY6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01837">
        <property type="entry name" value="SGNH_plant_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1110:FF:000003">
        <property type="entry name" value="GDSL esterase/lipase APG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050592">
        <property type="entry name" value="GDSL_lipolytic_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035669">
        <property type="entry name" value="SGNH_plant_lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45642">
        <property type="entry name" value="GDSL ESTERASE/LIPASE EXL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45642:SF35">
        <property type="entry name" value="GDSL ESTERASE_LIPASE APG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042131854" description="GDSL esterase/lipase APG" evidence="2">
        <location>
            <begin position="29"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7740690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="360" mass="38653" checksum="D3A07B8E1836D5BD" modified="2024-05-29" version="1">MFMASITKTVSWWLFVVAVAIIITIGEAQDSTSLVPAIIAFGDSAVDVGNNDYLATIFKANYPPYGRDFVNHQPTGRFCNGKLATDITADTLGFSTYPPAYLSPEATRKKLLIGANFASAGSGYDDKTAIGSHAIPLSQQLQYYKEYQGKLAQVAGSTKSASIIKDALYLISAGNSDFVQNYYVNPFINKVYTADQYGSYLIGIFTSFIKDLYGLGARRIGVTSLPPLGCLPAVRTLFGSHEKGCVASFNTDAQAFNKKVNAAATQLGKQLPGLKLVIFDIFQPLYDLVKDPASNGFLEATRGCCGTGRVETTVFLCNPKSVGTCSNATQFVFWDSVHPSEAANQVLADALILQGIALIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CH57</accession>
    <name>A0AAD5CH57_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_000879</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008207">
        <property type="protein sequence ID" value="KAI7741419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CH57"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd21039">
        <property type="entry name" value="NURR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041337">
        <property type="entry name" value="hnRNP_Q_AcD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21245">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18360">
        <property type="entry name" value="hnRNP_Q_AcD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="77"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="452"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7741419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="473" mass="52002" checksum="36733D8B94BFDDF7" modified="2024-05-29" version="1" fragment="single">CSEVVGSILPSGSEAALNKKFKNRGFGFVKFSSHAAAARALKFGSKPDFVLGGTLHPSVKWAEEEAGADLEELAKIKIAFVKNLPSSTDENYLKFVFGPFGKVEKVVVHNKGASAVGFIHFAQRSHLDNAIGGLNEKIVQGPKGGPSFKLQVEVARPMDKKRKRVYEDTQITQSREILNNTRPQIYEPFLSSFNGYHEDTVQKEPVVADPHEAAVLLLPVAVRDRLLRILRLGIATRYDIEVESLANLAELPESTAILVLDQFMLSGAEEHDKGAYLTSLISKHQVDKFGLNQLPFNMSRLRDTNVREPVGLGPSGRAHLPAVDSLGSVGLPVDPSVPRSDGFTSRYSSLISEYGLLPSHAGVTRRDERSPLHLHELPTSSSTASYSRYLTAYGNTEEMSPNPSQQIPVSSSISYANHAQDPYRIPAFVKGGDVAPTRPQMRFDPYTGEPYKFDPFTGEPIAPEIPYENTYRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CHI7</accession>
    <name>A0AAD5CHI7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Heat shock 70 kDa protein 17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_000586</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008134">
        <property type="protein sequence ID" value="KAI7741742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CHI7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034663">
        <property type="term" value="C:endoplasmic reticulum chaperone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030968">
        <property type="term" value="P:endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10230">
        <property type="entry name" value="ASKHA_NBD_HSP70_HYOU1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.10:FF:000002">
        <property type="entry name" value="Heat shock 70 kDa protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45639">
        <property type="entry name" value="HSC70CB, ISOFORM G-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45639:SF3">
        <property type="entry name" value="HYPOXIA UP-REGULATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041975362" description="Heat shock 70 kDa protein 17" evidence="8">
        <location>
            <begin position="25"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="557"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="778"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="557"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="571"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="782"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="807"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="834"/>
            <end position="850"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7741742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="850" mass="94452" checksum="C13272AB427FBE2C" modified="2024-05-29" version="1">MSTFLYKLGLTVFLLLLISSPSQSAVSSIDLGSEWIKVAVVNLKPGQAPISIAINEMSKRKSPALVAFHANDRLIGEEAAGLIARYPNKVYSQTRDLIGKPFGYVKKFLDSLYLPFDIVEDVRGGVGVRIDDGVTVYSVEELTGMVLGYGMKLAEFHSKVVVKDAVVTVPPYFGQVERKGLMQAAELAGVNVLALLNEHSGAALQYGIDKDFSNGSRYVVFYDMGASSTYAALVYFSAYNAKEFGKTVSVNQFQVKDVRWDPELGGQHMELRLVEYFADEFNKQVGNGVDVRQSPKAMAKLKKQVKLLIDSMSTITRQKFEEMCEDLWERSVIPVKELLKHSGLKADEIYALELIGGGTRVPKLQAKLQEFFGRSGVDRHLDADEATVLGASLHAANLSDGIKLNRKLGMIDGSMYGLMMDLDGPELVKDDTTTQLLVPRLKKLPIKMFRSITHNKDFDVSLLYENEDLLPPGVTSRTFAKYSVSGLADASEKYMSRNLSSPVKANLHFSLSRSGILSLDRADAVIEISEWVEVPKKNQTLENANVSAPLNLTVEAESANSAEASDALNPDDSTVSSANSTANGQNTVDLGTEKKLKKRTFRIPLKISEKSVGPVAPLSEDALYEVKLRLDALDAKDAERRRTAELKNDLEGYIYATKEKLDSLEELQKVSSSEQRQLFIEKLDEVQDWLYTDGEDASATQFQERLDQLKAIGDPIFFRYKELTARPEASQSAKRYFSELQEIVSGWETKKPWISKEKIDQVLREAENFKKWLNDKEDEQQKLSASSSPAYTSQEVANTNKIPKPKPKVEKPTVKNETKSQTDEPKSSSSSNDDDIKGETDGQSTEHDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CIY5</accession>
    <name>A0AAD5CIY5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Dipeptide epimerase</fullName>
            <ecNumber evidence="7">5.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_001033</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6 7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="6 7">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the mandelate racemase/muconate lactonizing enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.1.1.-" evidence="7"/>
    <dbReference type="EMBL" id="JAMZMK010007989">
        <property type="protein sequence ID" value="KAI7742439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CIY5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016855">
        <property type="term" value="F:racemase and epimerase activity, acting on amino acids and derivatives"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03319">
        <property type="entry name" value="L-Ala-DL-Glu_epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.120">
        <property type="entry name" value="Enolase-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.10">
        <property type="entry name" value="Enolase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034603">
        <property type="entry name" value="Dipeptide_epimerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036849">
        <property type="entry name" value="Enolase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029065">
        <property type="entry name" value="Enolase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013342">
        <property type="entry name" value="Mandelate_racemase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013341">
        <property type="entry name" value="Mandelate_racemase_N_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48073:SF2">
        <property type="entry name" value="O-SUCCINYLBENZOATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48073">
        <property type="entry name" value="O-SUCCINYLBENZOATE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13378">
        <property type="entry name" value="MR_MLE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02746">
        <property type="entry name" value="MR_MLE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00001">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00180">
        <property type="entry name" value="muconate_cycloisomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00009">
        <property type="entry name" value="o-succinylbenzoate_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00922">
        <property type="entry name" value="MR_MLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
        <property type="entry name" value="Enolase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="Enolase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3 6 7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 6 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mandelate racemase/muconate lactonizing enzyme C-terminal" evidence="8">
        <location>
            <begin position="184"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; specific for (R)-substrate epimerization" evidence="5">
        <location>
            <position position="205"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; specific for (S)-substrate epimerization" evidence="5">
        <location>
            <position position="313"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="260"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="289"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR634603-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR634603-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7742439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="407" mass="44046" checksum="D65CEEF212A45B64" modified="2024-05-29" version="1">MGSISFFSLFSPNLHKTRLTTVATMAHPTTSVGFKNLMESLTVDISRAEGRKLNVPLKAPFTIATSRLEGVENVAIRVELSNGCCGWGEAPILPFVTAEDQTTALTKAAEVSHFLKESDAMSLGDVLRGIGQRLPGHHFASVRAGVEMAVIDAVATSISTPLWRFFGGVSNTVTTDITIPIVSPTEAAQLASKYYKQGFKTLKLKVGKDLNADIEVLKAIRAAHPDCMFILDANEGYTSSEAIQVLEKLHEMEVTPILFEQPVHRDDWEGLGHVTRIAKTKYGVSVAADESCRSLADVKEIVKRQLADVVNIKLAKVGVLGALEIIEFAKASGLDLMIGGMVETRLAMGFAAHLAAGLGGFKYIDLDTPLLLSEDPVFEGYEVSGPTYKFTNARGHGGFLHWDNIAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CIZ4</accession>
    <name>A0AAD5CIZ4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_007139</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008141">
        <property type="protein sequence ID" value="KAI7741725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CIZ4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01837">
        <property type="entry name" value="SGNH_plant_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1110:FF:000003">
        <property type="entry name" value="GDSL esterase/lipase APG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051238">
        <property type="entry name" value="GDSL_esterase/lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035669">
        <property type="entry name" value="SGNH_plant_lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45650">
        <property type="entry name" value="GDSL-LIKE LIPASE/ACYLHYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45650:SF3">
        <property type="entry name" value="OS01G0748500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041947984" evidence="8">
        <location>
            <begin position="25"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7741725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="368" mass="40224" checksum="0D643C89E96F3C4B" modified="2024-05-29" version="1">MASEQKRWLVVYVIAIILALNNVAYEVNGDPQVPCYFIFGDSLVDNGNNNGIASLARANYLPYGIDFPDGPTGRFSNGKTVVDTVAELLGFDAYIPPYSDAGGRDILNGVNYASAAAGIRDETGQQLGARISFGGQVNNYKNTVSQVVDILGDEDSAASYLRQCIYSVGLGSNDYLNNYFMPNIYQTSSQYSPEEYATVLIQQYSELIRELYNYGARKVVLNGIGQIGCSPNALAQNSEDGNTCVEKINSANQMFNNLLKSLVNNLNSELTDAKFIYINNYDIFQDLINSPQSYGFTVTNAGCCGVGKNNGQITCLPGQTPCQNRDEHLFWDAFHPTEAANAIVGRRSYSAQSSSDAYPMDIRQLAQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CJG2</accession>
    <name>A0AAD5CJG2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ACB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_005364</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ACBP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008152">
        <property type="protein sequence ID" value="KAI7741666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CJG2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000062">
        <property type="term" value="F:fatty-acyl-CoA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056819">
        <property type="entry name" value="ACBP4-6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000582">
        <property type="entry name" value="Acyl-CoA-binding_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035984">
        <property type="entry name" value="Acyl-CoA-binding_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46093:SF3">
        <property type="entry name" value="ACYL-COA-BINDING DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46093">
        <property type="entry name" value="ACYL-COA-BINDING DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00887">
        <property type="entry name" value="ACBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24922">
        <property type="entry name" value="ACBP4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47027">
        <property type="entry name" value="Acyl-CoA binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51228">
        <property type="entry name" value="ACB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="4" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ACB" evidence="6">
        <location>
            <begin position="12"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="533"/>
            <end position="560"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7741666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="560" mass="60942" checksum="DD7B0791BBD75C15" modified="2024-05-29" version="1">MAMARVTSGLAYPDRFYAAATYAGFGSSSASVTSKFSNDVALLLYALYQQATVGPCNLPKPRAWSPVEHSKWTSWNGLGNMASSEAMRLFVKILEEEDPGWYSRVSDFIPEPVVDVEMNHNSKAELPIKNESTLPEVKTIPAENGNSVDKDVMVEVIGSVGVYDQWVAPPVSGPRPKPRYEVSRGGQSVTLVGNTLVIFGGQDGNRTLLNDLHILDLETMTWDEIDTIGVSPSPRSDHAAAVHAERYLLIFGGGTHSTCFNDLHVLDLKTMEWSKPSQQGEIPSPRAGHAGVTVGESWFIVGGGDNKSGASETVVLNMSTLSWSVVTTVQGRVPLASEGLSLVLSSCNGEDILVSFGGYNGRYNNEVNLLKPSYKSTLQATRSGVPALDSVSGAQNATNGTRDVDLEFEAGQEAKFREISMENKEPQTSEVNEVSERSLAALKAEKEELESALSNEKSQSNELKQELIEAESKNSDLYKELQSLRVQLAAEQSRCFKLEVDVAELRQKLQTMDSLQKELEILQRQKAASEEAYAKQKQSSGGVWSWLAGTPPPTQKATDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CLB1</accession>
    <name>A0AAD5CLB1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein ACCUMULATION AND REPLICATION OF CHLOROPLASTS 6, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_029899</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007635">
        <property type="protein sequence ID" value="KAI7743897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CLB1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009706">
        <property type="term" value="C:chloroplast inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010020">
        <property type="term" value="P:chloroplast fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058032">
        <property type="entry name" value="CDP1-like_a_solenoid_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057137">
        <property type="entry name" value="CDP1-like_a_solenoid_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025344">
        <property type="entry name" value="CDP1-like_IMS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044685">
        <property type="entry name" value="CPD1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33925">
        <property type="entry name" value="PLASTID DIVISION PROTEIN CDP1, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33925:SF1">
        <property type="entry name" value="PROTEIN ACCUMULATION AND REPLICATION OF CHLOROPLASTS 6, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23468">
        <property type="entry name" value="ARC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13355">
        <property type="entry name" value="ARC6-like_IMS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25515">
        <property type="entry name" value="Arm_PDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Plastid division protein CDP1-like 1st alpha solenoid" evidence="4">
        <location>
            <begin position="170"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="Plastid division protein CDP1-like 2nd alpha solenoid" evidence="3">
        <location>
            <begin position="331"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="domain" description="Plastid division protein CDP1-like IMS" evidence="2">
        <location>
            <begin position="665"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7743897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="787" mass="87028" checksum="B5D1A49AF0945582" modified="2024-05-29" version="1">MYSPMTSLTHLRTTTTFPPSTTTTVKKPSKPNAVNNTPIFTTFSASKWANRLFSDFQFLPTTDLTTTTTTTTATTTPPYPPPLSTPSRYVSMPIDFYRVLGAEPHFLGDGIRRCYEARVFKPPQYGYSDDALVSRRFILQAACETLADLSTRRQYNIGLADDEFGTLVTDVPWDNVPGALCVLQEAGEDELVLQIGENLLKDRLPKSFKQDVVLAMALAYIEISRDTMAMSPPDYIKGCEFLETALKLLQEEGASSLAPDLQAQIDETLEEINPRYVLELLALPLDDEYQSRRTEGLQGVRNILWAVGGGGAAAIAGGFTREDFMNEAFLRMTAAEQVELFTATPHNIPAESFEVYGVALAFVAQAFMGKKPRLIKEADNLFQQLQQIKVTALGNSASMYNARENRETDFALERGLCSLLVGEVDECRSWLGLNNDNSPYREPSIAAFVMENSRNDPDNDLLPGLCKLLETWLIEVVFPRFRDTTDVQFKLGDYYDDPIVLRYLERLEGVSGSPLAAAAAIAKIGAEATAVLDSVKVGALQALQKVFPLGQKAESVKRQNNSEFDDSFVAETDDIISEENNLNGNQEQEIITYQIKDAAVKIMCAGVVVGLMTLAGLKFVPFKRGSTSTTAHNEVGSALASDTINVGDSVVEDAEEIPKMDARLAEGLVRKWQSIKSQALGPDHCHSKLSEVLDGEMHKIWLQRATEIAQRGWFWEYTLLNITIDSVTVSLDGRLAVVEATLEESAKLIDLTHPENNDSYNLTYTTRYEMSCAKSSWKITKGAVLKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CM09</accession>
    <name>A0AAD5CM09_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_032666</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007835">
        <property type="protein sequence ID" value="KAI7743001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CM09"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="123"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="129"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="141"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7743001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="151" mass="17320" checksum="809E8B06C51E6115" modified="2024-05-29" version="1" fragment="single">MESEQLIRSGGSGSHYIQMQTEPSRLSSFSFHQNPPESTRIFDELPKATIVQVSRPDAGDISPMLLSYTIDFQYKQFKWQLVKKASHLFYLHFALKKRALIEEIHEKQEQVKEWLQNLGIGDQPAVVHDDDDDDEPDDTVMVSNNEGSVKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CPU1</accession>
    <name>A0AAD5CPU1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">FLZ-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_017253</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FLZ family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007051">
        <property type="protein sequence ID" value="KAI7746043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CPU1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044533">
        <property type="entry name" value="FLZ1/2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007650">
        <property type="entry name" value="Zf-FLZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46057">
        <property type="entry name" value="FCS-LIKE ZINC FINGER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46057:SF48">
        <property type="entry name" value="ZF-FLZ DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04570">
        <property type="entry name" value="zf-FLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51795">
        <property type="entry name" value="ZF_FLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FLZ-type" evidence="6">
        <location>
            <begin position="15"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="FLZ-type" evidence="4">
        <location>
            <begin position="15"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="62"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="80"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7746043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="89" mass="10119" checksum="0C65D58CEEFB2795" modified="2024-05-29" version="1">MSYDALFYAGSEEPHFLDACTLCSKPLSHNSDIFMYRGNTPFCSQECRQEQIENDEARERRWKKMASKKSTETSKKSATVQTGTLVASN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CQY8</accession>
    <name>A0AAD5CQY8_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_029415</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006938">
        <property type="protein sequence ID" value="KAI7746611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CQY8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47261">
        <property type="entry name" value="CALCIUM-DEPENDENT LIPID-BINDING (CALB DOMAIN) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47261:SF2">
        <property type="entry name" value="CALCIUM-DEPENDENT LIPID-BINDING (CALB DOMAIN) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="147"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="114"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="121"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7746611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="173" mass="18628" checksum="05E62787FA9A00B3" modified="2024-05-29" version="1" fragment="single">SLKDTVPTDKIVALRGGWGLFRKGYAGEILLRLTYKAYVEDEEDEKAETVTIDTDISDDELSDDLEQAAATYGQRVNDFASGTNREAFMDVLAALLVSEEFQGIVASETSNAKYPTDVKNSESTDTSRGAKSAPDTSNSESGSRGQVLLWLAVITSVSILITFDVGGSSIFNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CRS5</accession>
    <name>A0AAD5CRS5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">aconitate hydratase</fullName>
            <ecNumber evidence="3">4.2.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_016167</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>citrate = D-threo-isocitrate</text>
            <dbReference type="Rhea" id="RHEA:10336"/>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
            <dbReference type="ChEBI" id="CHEBI:16947"/>
            <dbReference type="EC" id="4.2.1.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the aconitase/IPM isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.3" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010007214">
        <property type="protein sequence ID" value="KAI7745520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003994">
        <property type="term" value="F:aconitate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006101">
        <property type="term" value="P:citrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006102">
        <property type="term" value="P:isocitrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01586">
        <property type="entry name" value="AcnA_IRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01580">
        <property type="entry name" value="AcnA_IRP_Swivel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.19.10:FF:000001">
        <property type="entry name" value="Aconitate hydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.499.10:FF:000002">
        <property type="entry name" value="Aconitate hydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.499.10:FF:000005">
        <property type="entry name" value="cytoplasmic aconitate hydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.499.10">
        <property type="entry name" value="Aconitase, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.19.10">
        <property type="entry name" value="Aconitase, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044137">
        <property type="entry name" value="AcnA_IRP_Swivel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015931">
        <property type="entry name" value="Acnase/IPM_dHydase_lsu_aba_1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001030">
        <property type="entry name" value="Acoase/IPM_deHydtase_lsu_aba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015928">
        <property type="entry name" value="Aconitase/3IPM_dehydase_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006249">
        <property type="entry name" value="Aconitase/IRP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018136">
        <property type="entry name" value="Aconitase_4Fe-4S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036008">
        <property type="entry name" value="Aconitase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000573">
        <property type="entry name" value="AconitaseA/IPMdHydase_ssu_swvl"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006757">
        <property type="entry name" value="PRK09277.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11670">
        <property type="entry name" value="ACONITASE/IRON-RESPONSIVE ELEMENT FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00330">
        <property type="entry name" value="Aconitase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00694">
        <property type="entry name" value="Aconitase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00415">
        <property type="entry name" value="ACONITASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53732">
        <property type="entry name" value="Aconitase iron-sulfur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52016">
        <property type="entry name" value="LeuD/IlvD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00450">
        <property type="entry name" value="ACONITASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01244">
        <property type="entry name" value="ACONITASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aconitase/3-isopropylmalate dehydratase large subunit alpha/beta/alpha" evidence="10">
        <location>
            <begin position="65"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="Aconitase/3-isopropylmalate dehydratase large subunit alpha/beta/alpha" evidence="10">
        <location>
            <begin position="346"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="domain" description="Aconitase A/isopropylmalate dehydratase small subunit swivel" evidence="11">
        <location>
            <begin position="665"/>
            <end position="793"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7745520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="870" mass="94836" checksum="56453A72E0E435DC" modified="2024-05-29" version="1">MASENPFKGILTTLPRPGGGEFGKFYSLPALNDPRIDKLPYSIRILLESAIRNCDNFQVTRNDVEKIIDWENTSPKQVEIPFKPTRILLHDYTAVPAVVDLACMRDAMNKLGGDSKLINPLVPVDLVIDHSVQVNVSRSANAVQANMDLEFQRNNERFTFLKWGSHAFDNMLVVPPGSGIIHQVNLEYLGRIVFNTDGILYPDSVVGTDSHTTMIDGLGVAGWGVGGIEAEATMLGQPMSMVLPGVVGFKLSGKLQNGITATDLVLTVTQMLRKHGVVGKFVEFYGEGMSELSLADRATIANMSPEYGATMGFFPVDHVTLQYLKLTGRSDETHTCEQTICLSIIARPHDRVLIKDMKADWHSCLDNKVGFKGFAVPKETQDKVVSFPFHGQTAELKHGSVVIAAITSCTNTSNPSVLVGAGLVAKKASELGLEVKPWIKTSLAPGSGVVTKYLLKSGLQKYLDQQGFSTVGYGCTTCIGNSGDLDESVAAAITDNNIVAAAVLSGNRNFEGRVHPLTRANYLASPPLVVAYALAGTVDIDFEKEPIGTTKEGKSVYFKDIWPSNEEIAQVVQSSVLPDMFKSIYQAITQGNPMWNKLPVPSSSLYSWDPNSTYIHEPPYFKDMTMNPPGPHVMKDTYCLLNLGDSITTDHISPAGSIHKDSPAAKYLLDHGVDRRDFNSYGSRRGNDEVMARGTFANIRIVNKLLNGEIGPKTIHXXXXDKLSVFDVAMRYKEAGHATIVLAGSEYGSGSSRDWAAKGPMLLGVKVVIAKSFERIHRSNLVGMGVLPLCFKAGQDADTLGLTGYERYTIDLPAKASDIKPGQDVTVTTDNGKSFTCTVRFETQVELAYFDHGGILPYVIRNLIKKQKQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CSB5</accession>
    <name>A0AAD5CSB5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">At4g15545-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_022192</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007154">
        <property type="protein sequence ID" value="KAI7745715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CSB5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058936">
        <property type="entry name" value="At4g15545-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058935">
        <property type="entry name" value="At4g15545-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47383:SF8">
        <property type="entry name" value="OS01G0768300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47383">
        <property type="entry name" value="OS03G0659800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25972">
        <property type="entry name" value="At4g15545_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="At4g15545-like C-terminal" evidence="2">
        <location>
            <begin position="274"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="161"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="206"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="178"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="209"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="229"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="247"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7745715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="341" mass="37962" checksum="44145C94935BC043" modified="2024-05-29" version="1">MMSEHSTASSTSITTTNGPDFNLPDEILSVIPTDPYDQLDLARKITSMALASRVSKLESETASLRHKLNERDRVVMELEEKVSELHQTCRNAQLRLQLTVEDNRKLVEERDSLAVTAKKLGRDLAKLEAFKRQLMQSLNDNDSAPAAPVDIGTYDQSAPKAYSVKEEETNGYRRHHSYSGSIDSGNIQDDASKLAGQRFSMTPYISTRVTPTATPKTASASVSPRRYSASGTTSPTKIQYEGRTTMSSWYPSSQQSSAGNSPPRGPPIPGRTAKIDGKEVFRQARSRLSYEQFSAFLANIKELNAQKQSREETLRKAEEIFGSDNKDLYLSFQALLNRNKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CSK6</accession>
    <name>A0AAD5CSK6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Adenylosuccinate synthetase, chloroplastic</fullName>
            <shortName evidence="7">AMPSase</shortName>
            <shortName evidence="7">AdSS</shortName>
            <ecNumber evidence="7">6.3.4.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">IMP--aspartate ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">PURA</name>
        <name evidence="9" type="ORF">M8C21_025889</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Plays an important role in the de novo pathway and in the salvage pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP.</text>
    </comment>
    <comment type="function">
        <text evidence="8">Plays an important role in the de novo pathway of purine nucleotide biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>IMP + L-aspartate + GTP = N(6)-(1,2-dicarboxyethyl)-AMP + GDP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:15753"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57567"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="6.3.4.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="7">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7 8">Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Plastid</location>
            <location evidence="7">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 8">Belongs to the adenylosuccinate synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.4" evidence="7"/>
    <dbReference type="EMBL" id="JAMZMK010006748">
        <property type="protein sequence ID" value="KAI7747473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CSK6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004019">
        <property type="term" value="F:adenylosuccinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044208">
        <property type="term" value="P:'de novo' AMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046040">
        <property type="term" value="P:IMP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.440.10">
        <property type="entry name" value="Adenylosuccinate Synthetase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.170.10">
        <property type="entry name" value="Adenylosuccinate Synthetase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00011">
        <property type="entry name" value="Adenylosucc_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018220">
        <property type="entry name" value="Adenylosuccin_syn_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042109">
        <property type="entry name" value="Adenylosuccinate_synth_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042111">
        <property type="entry name" value="Adenylosuccinate_synth_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001114">
        <property type="entry name" value="Adenylosuccinate_synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11846">
        <property type="entry name" value="ADENYLOSUCCINATE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11846:SF15">
        <property type="entry name" value="ADENYLOSUCCINATE SYNTHETASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00709">
        <property type="entry name" value="Adenylsucc_synt"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00788">
        <property type="entry name" value="Adenylsucc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01266">
        <property type="entry name" value="ADENYLOSUCCIN_SYN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="6 7 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1 7 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 7 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 7 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0934">Plastid</keyword>
    <keyword evidence="4 7 8" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="84"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="112"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="83"/>
            <end position="89"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="84"/>
            <end position="87"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="109"/>
            <end position="112"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="111"/>
            <end position="113"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7747473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="268" mass="29439" checksum="29BCF82FD1848100" modified="2024-05-29" version="1">MTTNSVNLHTNPTTSPPFRSVTATGRTYTTTVFRRTFLHPHRINASIKCSASPIVAAEERTGQIPTRIESLGQVSAVLGCQWGDEGKGKLVDILAKHFDVVARCQGGANAGHTIYNSEGKKFALHLVPSGILNEETMCVIGNGVVVHLPGLFKEIDGLESNGVSCKGRILVSDRAHLLFDYHQEIDGLREAELAKNNRECRLQLGLCSKWVEVEYETVPGWQTDISSVRSYSDLPKAAREYVERIEQLVGVPIHYIGVGPGRDALIYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CTN4</accession>
    <name>A0AAD5CTN4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_029027</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006707">
        <property type="protein sequence ID" value="KAI7747689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CTN4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="89"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7747689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="139" mass="15800" checksum="3B4023CF5DE2D1E3" modified="2024-05-29" version="1" fragment="single">VNVHPSKQKSGFLPLFASHIPPPTIPALLRSAPNRGRRRNQRQIESGNFASSTTGVKVLRVLRIHELLALCQAAVDRDKVQMCSPYKTTVFRPPFIGLFLLVFYFNLPNVIHMNVKESIVTIKVFDEMRERGKGRKFVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CUE8</accession>
    <name>A0AAD5CUE8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phospholipid/glycerol acyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_008527</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GPAT/DAPAT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006929">
        <property type="protein sequence ID" value="KAI7746630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CUE8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090447">
        <property type="term" value="F:glycerol-3-phosphate 2-O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010143">
        <property type="term" value="P:cutin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06551">
        <property type="entry name" value="LPLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1440.100:FF:000004">
        <property type="entry name" value="Glycerol-3-phosphate 2-O-acyltransferase 6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.100">
        <property type="entry name" value="SG protein - dephosphorylation function"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056462">
        <property type="entry name" value="HAD_RAM2/GPAT1-8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15486">
        <property type="entry name" value="ANCIENT UBIQUITOUS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15486:SF91">
        <property type="entry name" value="PHOSPHOLIPID_GLYCEROL ACYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23270">
        <property type="entry name" value="HAD_RAM2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="253"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="9">
        <location>
            <begin position="312"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7746630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="525" mass="58291" checksum="02F445343A089C81" modified="2024-05-29" version="1">MADNHKNSDHSCEFPAIATCKTHGRDKHTVVSDLDGTLLRGHTSFPYFALVAFEVGGPLRLLFLLLASPIAGILYYFISEAAGIRVLIFATMAGMKVSHIESVARAVLPKFYSSDLHPEAWRVFVACGKRCVLTANPTLMVEPFLKEFLGADMVLGTELDSWRGRATGLVKPPGVLVGIHKANALMKAFKDASLPELALGDRESDYPYMKLCKEAYLVPQAPAELKAVTVDKLPKPVVFHDGRLVHKPTPLKALITIIWIPIGFLLACLRIAAGALLPMSLVYYAIWALGVRVYVKGTPPPPTKKSTGQTGVLFICSHRTLLDPVFLSIALRRPIPAVTYSLSRLSEIMSPIKTVRLTRDKTSDANMIKKLLTQGDLVICPEGTTCREPILLRFSSLFAKLTNELVPVAMSNKMSMFHGTTARGWKWMDSFYFLMNPSPAYEVTFLNKLPYELTCASGQLSHDVANYIQRVIASSLSYECTNFTRKDKYKMLAGNDGTVDTRKPKVAMLKISWDDRKWMEMSYCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CUQ0</accession>
    <name>A0AAD5CUQ0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_000373</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the COBRA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006571">
        <property type="protein sequence ID" value="KAI7748259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CUQ0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016624">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010215">
        <property type="term" value="P:cellulose microfibril organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052324">
        <property type="term" value="P:plant-type cell wall cellulose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006918">
        <property type="entry name" value="COBRA_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31673:SF48">
        <property type="entry name" value="COBRA, PLANT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31673">
        <property type="entry name" value="PROTEIN COBRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04833">
        <property type="entry name" value="COBRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7748259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="205" mass="22125" checksum="90F83E830D1F6929" modified="2024-05-29" version="1" fragment="single">AVVTMNNFQQYRHISPPGWTLGWTWAKKEIIWSMMGGQATEPGTPYNQPISNCCKGGVINSWAQDPNNYASSFQVSVGAAGTTNKTVKPPKNFTLLAPGPGYTCGPAIVGKRTKLITADGRRVNQAMSDGVVLRGESYGIPSIRGDGDDALAIYSVAYEARKMSIEKQTPVLIEALTYRTGHHSTSDDSTSIAWLKKLNGGELET</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CUY3</accession>
    <name>A0AAD5CUY3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_012876</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006605">
        <property type="protein sequence ID" value="KAI7748087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CUY3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990428">
        <property type="term" value="P:miRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21608">
        <property type="entry name" value="RRM2_NsCP33_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050886">
        <property type="entry name" value="RNA-binding_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048289">
        <property type="entry name" value="RRM2_NsCP33-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48024">
        <property type="entry name" value="GEO13361P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48024:SF56">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN A0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="8"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7748087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="107" mass="11888" checksum="D7221D6BC1406AAF" modified="2024-05-29" version="1">MSSADVEYRCFVGGLAWATTDQSLEEAFKPYGEVLDSKIINDRETGRSRGFGFVTFKDEHSLREAIEGMNGQSLDGRNITSDLNSEVAFLIFCDRGCLQTKPNPDIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CVW0</accession>
    <name>A0AAD5CVW0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">VDE lipocalin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_012112</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006565">
        <property type="protein sequence ID" value="KAI7748305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CVW0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046422">
        <property type="term" value="F:violaxanthin de-epoxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010028">
        <property type="term" value="P:xanthophyll cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012674">
        <property type="entry name" value="Calycin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044682">
        <property type="entry name" value="VDE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010788">
        <property type="entry name" value="VDE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33970:SF2">
        <property type="entry name" value="OS01G0716400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33970">
        <property type="entry name" value="VIOLAXANTHIN DE-EPOXIDASE, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07137">
        <property type="entry name" value="VDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VDE lipocalin" evidence="1">
        <location>
            <begin position="154"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7748305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="412" mass="46851" checksum="E68206C9487206BC" modified="2024-05-29" version="1">MSFLTLSPLSLNLLLNRNNNRPSLSFHVSRTCTSPPYLRHKFTFSKLRTVCSALQTNDGVSSDLCSTEPRGFVSDVRIVAVVGEGAVSPLKSASWLDVMSHTAERLKWIDDTYEMLVFTDNISESNHNVQNLQKETMELAETISEAWIRNNSDDIRKALQCLNQCSPVDQVCNYKCIASYESPILEEFSLCVLQKHNCLDLDAKIPEKPSVSPMVNFRDKVLSHEIAEDLFVGWLGNLEWSWRVVAGQNPAYDQFPCQYQLFYRGKARGSFWYEPVFQVKTLDGELVWRRRKYRVKRGKVAGTFYFSVLDNGVVSNEYWTIVDVCEDFSWALFHYSGAARVAGQSYTGAVLVSQDGEYPNEMEKDRLVSALDRCGIKEWELFDVDNCSCKDAPLGLPEGSRLHMTVMPDDCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CYV1</accession>
    <name>A0AAD5CYV1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_025945</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006064">
        <property type="protein sequence ID" value="KAI7750893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CYV1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.50:FF:000006">
        <property type="entry name" value="Fringe-related protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006740">
        <property type="entry name" value="DUF604"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10811">
        <property type="entry name" value="FRINGE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04646">
        <property type="entry name" value="DUF604"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7750893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="469" mass="53556" checksum="572FCA7EA48D457D" modified="2024-05-29" version="1">MPIFKTVPSKIKDICLIISSLSIFYLLSHPLTPLTPSVPSVTTVITPPPLTSRHVLFSIASSSKSYINRKPYLHLWYNPNSTNAITFLDRPISHVTNPNPNLPPIIISSDTSHFPYTFPKGLRSAIRVARIVKEAVDYVTVTESLKDNIRWYVFGDDDTVFVRENVVRVLSKYDDKKWYYIGGRSESYDQNMQHSFNMAFGGGGFAISGSLGRVLGRVLDSCLRRYPHVYGSDSRVYSCLVELGVELTYEPGFHQVDVRGDLFGILAAHPLSPLLSLHHFDIIDPIFPGLTKQESVKHLFKAVNHDPPRILQQAVCYDSSKSITISVSWGYVVQVFEGNQLLPDLIRVQKTFTSWRRKETSFSRLHMFDMRDYSKDPCKSPDLFFFEGIVPEEKRSHTFYTRKKSMNCSRSEGIRNLVNIKVFSRKIDADTQQISPPRRECCEILQPYNETMVIALRRCEIDELIAMDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CZT8</accession>
    <name>A0AAD5CZT8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Alcohol dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_028928</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006150">
        <property type="protein sequence ID" value="KAI7750457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CZT8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051903">
        <property type="term" value="F:S-(hydroxymethyl)glutathione dehydrogenase [NAD(P)+] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046294">
        <property type="term" value="P:formaldehyde catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.180.10:FF:000007">
        <property type="entry name" value="Alcohol dehydrogenase 6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000003">
        <property type="entry name" value="S-(hydroxymethyl)glutathione dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43880">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43880:SF49">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE SUPERFAMILY, ZINC-TYPE, GROES-LIKE, NAD(P)-BINDING DOMAIN PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alcohol dehydrogenase-like N-terminal" evidence="10">
        <location>
            <begin position="41"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="Alcohol dehydrogenase-like C-terminal" evidence="9">
        <location>
            <begin position="211"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="Alcohol dehydrogenase-like C-terminal" evidence="9">
        <location>
            <begin position="453"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="domain" description="Alcohol dehydrogenase-like N-terminal" evidence="10">
        <location>
            <begin position="592"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="domain" description="Alcohol dehydrogenase-like C-terminal" evidence="9">
        <location>
            <begin position="763"/>
            <end position="894"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7750457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="939" mass="102395" checksum="C0B9CDCF1EEB2797" modified="2024-05-29" version="1">MDGKCSSNTAGQPIRCKAAIVRKAGEPLVIEEVIVAPPKHREVRIKIICTSLCHSDLSFWKLTDPPAIFPRILGHEAVGIVESVGEGVHEVVEGDTVIPIFLADCGECTDCLSEKSNLCTKFPFNLSPWMEREETSRFTDINGEPLYHFLFVSSFSEYTVVDIAHLVKSDPAIPPNRACLLSCGVSTGVGAAWKTAKVEAGSTVAIFGLGAIGLAVAEGARLCGAKRIIGVDVNQDKFEIGKKFGVTDFVNPGNCGDKTVSQIILEITGGGADYCFECVGMKSLVHEAYASCRKGWGKVVVLGVDHPEAMLTFNSFEVLHSGKSIMGSFFGGLKPKSDIRILEKRYMDKEICLDEFVTHEVDFKDINKAFDLMTEGKTAIVRKPGEPLVIEEVIVAPPKRCEVRIKIICTVHSDFTIWKFKDPPAVFPRILGHEAVGIVESIGEGVNEVVEGDTIIMEMTGSGADYCFECVGMKSLVHEAYASCRKGWGKVVVVGVDHPEAMLSFNSFEVLHSGKTIMGSFFGGLKPKSDISILEKRYINKEINLDEFVTHEVDFEDINKAFDLMTEGKTAIVRKAGEPLVIEEVFVAPPKAREVRIKIICTSLCHSDVTFWKLQHPPAIFPRILGHEAVGVVESVGEGVHEVVEGDTVIPIFLPDCGECTDCLSVKSNLCSKLPFSVSPYIDREKTSRFTDINGETLYIFLFVSSFSEYTVVDIGHVTKIDPQTPPNRACLLSCGVSTGVGAAWKTANVEAGTTVAIFGLGAIGLAVAEGARLCGAKRIIGVDVNQDKFELGKKFGVTDFVNSRNCGDKSVSQVIMEMTGDGADYCFECVGMSSLVHEAYASCRKGWGKTVVLGVDQPGAKLTFTSYEVLHSGKSIMGSLFGGIKPKSDIPILIKRYMDNELQLDEFVTHEVEFEDINKAFDLLLEGKSLRCVIWMKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D0S1</accession>
    <name>A0AAD5D0S1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_028150</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="JAMZMK010005936">
        <property type="protein sequence ID" value="KAI7751292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D0S1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000060">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002">
        <property type="entry name" value="RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE SD1-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002:SF932">
        <property type="entry name" value="RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="1"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7751292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="219" mass="24657" checksum="0DB0F55898383990" modified="2024-05-29" version="1">MLNWPQRFNIIHGIARGILYLHQDSRLQIIHRDLKASNVLLDGDMNPKISDFGLARNFVGRDTTAKTKKVVGTHGYIPPEYAVHGHFSIKSDVFSFGVLVLEIICGKRNREFFHGDHSDNLLGHAWRLYKEGRSSELLNTSLQASCVVSEVLRSIHIALLCVQHHPEDRPTMWSVVLMLVSDGPLPKPKTPAFFTEESCGELKSLPSVDECTITLVYAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D259</accession>
    <name>A0AAD5D259_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">WAT1-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_015334</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005784">
        <property type="protein sequence ID" value="KAI7751982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D259"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030184">
        <property type="entry name" value="WAT1-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31218">
        <property type="entry name" value="WAT1-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="45"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="76"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="111"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="141"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7751982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="172" mass="18811" checksum="6B621ED46C550D1F" modified="2024-05-29" version="1">MEKVHLRSYTSHMKIVGTFLSISGAIIATVYSGPSLLSASMNTDWIIGGILIAGQYFLVSFCLIAQAKILLEYSVVLVVACVLNTCELFVAGLAALIMARDLEAWKLRLDVVLASTLYVGFFFGFVNVLVQFWVLRLKGPLYVAMFKPLSIIIAVIMGVLILGDPVYLGRLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D332</accession>
    <name>A0AAD5D332_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_031133</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005811">
        <property type="protein sequence ID" value="KAI7751804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D332"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7751804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="216" mass="23902" checksum="0BB00699791923A3" modified="2024-05-29" version="1" fragment="single">ILSARRRRCRWGSPLSLRRGRGCLGDKMVRGRGAHSEGEGEDEGGVRVKVGLEVMMMMRRRGCLIMHMNNNMVVADIPVPPQPDVPPQPPLDSPQQHWVRWAVVIGPHIPEPCSSYACEEDHASGFPFIPRQHVVTIQIKATAFPLFFSQFIPFFSNTVANSPCSRHGIQLTIISFLGGGQVIARTMMVEALIDKGYNSTAAIIILAARDVNFDDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D3R5</accession>
    <name>A0AAD5D3R5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PGG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_015117</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005722">
        <property type="protein sequence ID" value="KAI7752209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D3R5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026961">
        <property type="entry name" value="PGG_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24177:SF362">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING DOMAIN, PGG DOMAIN PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24177">
        <property type="entry name" value="CASKIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13962">
        <property type="entry name" value="PGG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="190"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="228"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="273"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="301"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="PGG" evidence="2">
        <location>
            <begin position="180"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7752209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="347" mass="39895" checksum="503C7B5C2D64DF46" modified="2024-05-29" version="1">MDLLRIVWENISTMNKSKIDDILRGRPLWNENAIKGYPSRVLFLAVKMGNTSFVIELIRSYPDLIWEQDDKGKTIFHLAAKQRQAEIYNLLYETGGRKKLIIRIEDKNGNNILHMAGMRPKHTYRKAYGGAAIQLQQELLWFTHVWLRIPRHYTNKKNKAGETPLELFSRTHADLLTEAKKWMKETSAQCMVATTLITTIVFTSAFTLPGGYNQNNGIPFFRKEPSFIIFVIADAVSLVSASTSVLLFLSILTSGYGEDEFFASLPSKLANGLITLFLSVLSMLVAFAANFFVLYNKDLEWMPVTITCLASFPVILFVILQYRLFKNVFYSNYRLRSNFRPKRILYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D4S3</accession>
    <name>A0AAD5D4S3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Epidermal patterning factor-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_009242</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Controls stomatal patterning.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the plant cysteine rich small secretory peptide family. Epidermal patterning factor subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005766">
        <property type="protein sequence ID" value="KAI7752061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D4S3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010052">
        <property type="term" value="P:guard cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039455">
        <property type="entry name" value="EPFL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33109">
        <property type="entry name" value="EPIDERMAL PATTERNING FACTOR-LIKE PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33109:SF60">
        <property type="entry name" value="EPIDERMAL PATTERNING FACTOR-LIKE PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17181">
        <property type="entry name" value="EPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041782197" description="Epidermal patterning factor-like protein" evidence="7">
        <location>
            <begin position="34"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7752061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="107" mass="12088" checksum="BEFCF74704837B05" modified="2024-05-29" version="1">MAPTRTYLLGPPKTVNVPAILLLFLTLSHSISGYGDEGVASVEENKKVLGSRPPRCVNKCVSCRPCEATLVIPPHYKTIRYRDQSHYEDNYYLLSWKCKCGDKLFQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D523</accession>
    <name>A0AAD5D523_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PGG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_015119</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005722">
        <property type="protein sequence ID" value="KAI7752211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D523"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026961">
        <property type="entry name" value="PGG_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24177:SF362">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING DOMAIN, PGG DOMAIN PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24177">
        <property type="entry name" value="CASKIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13962">
        <property type="entry name" value="PGG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="523"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="561"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="606"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="634"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="domain" description="PGG" evidence="3">
        <location>
            <begin position="514"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7752211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="682" mass="77026" checksum="4437118BF4E6266B" modified="2024-05-29" version="1">MNNTGAGSGSGTPPVPINHNHHQQPNHTTVPIASPPAPLPPNLPGSDLLEAERMNHPSTSSGHAIPVNQQQPNDAIVQISQQSMPPSVPPQRNQPSLDILQGSREEYVKVAVPLYEAAMKGDWKAAKTIIDEHPNVIRSAITENEETLLHIAASAESTTAVEEFVINLVNMMDQKDLELQNKSWDTALSSAAAAGNIETAKAMVGKNPALTEMHNQRNVMPLYMAALFAKPDMVRYLYGISNKMVGDFWTNDNRGWVLQKCVEADMFDVALKILSECPNLILTKGLLTEVLLALAQKPDAFRRKKTNIIFTYINSIFAVSSKKETQALRLLKIIWAKVAIMPKDDIDFIIRGPATTIFTDRERRMQKVVHRSRVLFVAAKMGNTRFIIELIRLYPDLIWKQDDKKKTIFHLAVKRRQVEIYKLLHEIGAMKDLITPMKDMYGNNMLHMVAKSVKQKRFQNVSGVAFQMQSELLWFQEVEKMIPPNYRQKKNNEGKTPQDLFTKMHEKLLKDGGDWMKHTASQCMVVATLIATIVFAAAFTLPGGYDQDTGIPFFRREASLIIFVISDAISLICSTTSVLMFLSILTSRYAERDFLEALPRKLVNGITTLFLSIVTMMIAFSASFFVLYHKKLKWMPITITALASVPVVFYVFLQQDLFRDVVKAAYQSKHLFKRNNKRTLYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D626</accession>
    <name>A0AAD5D626_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transcription factor HY5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_016352</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bZIP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010002137">
        <property type="protein sequence ID" value="KAI7754988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D626"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009585">
        <property type="term" value="P:red, far-red light phototransduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010099">
        <property type="term" value="P:regulation of photomorphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010218">
        <property type="term" value="P:response to far red light"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010114">
        <property type="term" value="P:response to red light"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14704">
        <property type="entry name" value="bZIP_HY5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.490:FF:000004">
        <property type="entry name" value="Transcription factor HY5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.490">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044280">
        <property type="entry name" value="Hac1/HY5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46714">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR HAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46714:SF6">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR HAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-0607">Phytochrome signaling pathway</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="BZIP" evidence="12">
        <location>
            <begin position="87"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="37"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="51"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00070194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00084091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7754988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="196" mass="22076" checksum="85C4A9BA8C448AB8" modified="2024-05-29" version="1" fragment="single">DQAATSSVAASLPSSSERSSSSALQANVKEGMESDDEIRKVPEIVREPETATASGTGRETVSVSNPHRAQGAVEGTRRKGKSPADKESKRLKRLLRNRVSAQQARERKKAYLSELEVRVKELEKKNSELDERLSTLQNENQMLRHVCIYNNIYLYMYICIFVCIYGCCIEDVDWIVNCCLCMQILKNTTAGIQERK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D729</accession>
    <name>A0AAD5D729_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_019732</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010004125">
        <property type="protein sequence ID" value="KAI7754207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D729"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7754207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="79" mass="9037" checksum="A973B0D0A39BCC47" modified="2024-05-29" version="1">MHRLKQQFLLAVEKKEMKMMQGKESKGGSGLSLLGLAQELFSHPQEGNRDALSLLYAHPYMHSQTQRTFHPVLCLQEKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D8A2</accession>
    <name>A0AAD5D8A2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_004020</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the plant ACBP60 protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010002517">
        <property type="protein sequence ID" value="KAI7754774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D8A2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080142">
        <property type="term" value="P:regulation of salicylic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046829">
        <property type="entry name" value="Calmod_bind_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046830">
        <property type="entry name" value="Calmod_bind_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012416">
        <property type="entry name" value="CBP60"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31713:SF79">
        <property type="entry name" value="CALMODULIN-BINDING PROTEIN60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31713">
        <property type="entry name" value="OS02G0177800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20452">
        <property type="entry name" value="Calmod_bind_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20451">
        <property type="entry name" value="Calmod_bind_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Calmodulin binding protein central" evidence="8">
        <location>
            <begin position="10"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="Calmodulin binding protein C-terminal" evidence="9">
        <location>
            <begin position="37"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF20452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7754774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="274" mass="30717" checksum="BCA7CA32374F5850" modified="2024-05-29" version="1" fragment="single">TCTETVYALQILGSGMSTKMWEAVVEHARRCMVDDKKLYLYCPSSLNRDGVVFNVVGQVLGVLSDSRYVVADNLSDLERDDAHKLVISAFQHQESIISYDDEASLRTGTCSISEDLYPSDRQIITGDPEGSKIRTSHKKGRFDYPQMSALSPDVNVMSTIFPLGGIDSLDEYGLSSMETDELRFDHDQTLNLPNEALICDSKSLKGHDDEDMQYFGTSSSSHVDLQRAVDGFLFPHSAIGKAQRRWKIVSSVLKWLSLMLEIRERDIMKCMNPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DBS5</accession>
    <name>A0AAD5DBS5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_009647</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000010">
        <property type="protein sequence ID" value="KAI7758288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DBS5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7758288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="84" mass="9008" checksum="53ACE77C14A3808B" modified="2024-05-29" version="1" fragment="single">TREINPSLHRLGIPVVGLAAAMNQRPVTKSFCCEIGGSDASPPTTVTMIVTLTAIEQIPARSVTYVDVILQSIDRQGEFVRNGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DFC6</accession>
    <name>A0AAD5DFC6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">CASP-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_009378</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3 8">Homodimer and heterodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Casparian strip membrane proteins (CASP) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000278">
        <property type="protein sequence ID" value="KAI7756725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DFC6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006459">
        <property type="entry name" value="CASP/CASPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006702">
        <property type="entry name" value="CASP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044173">
        <property type="entry name" value="CASPL"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01569">
        <property type="entry name" value="A_tha_TIGR01569"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36488">
        <property type="entry name" value="CASP-LIKE PROTEIN 1U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36488:SF8">
        <property type="entry name" value="CASP-LIKE PROTEIN 1U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04535">
        <property type="entry name" value="CASP_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="37"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="83"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="121"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="168"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="Casparian strip membrane protein" evidence="10">
        <location>
            <begin position="34"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7756725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="202" mass="21506" checksum="BA1F867450086E12" modified="2024-05-29" version="1">MAAVNTSHSSVDMPTPESEYKGSTGTGGVKNHVVVDVVLRVMLFGTSLVAMILMVTSKQTKLVPVAPGMAIPMVANFNQTPSFIYFVAAMSVACLYSLITGALSVLALLKLKRVSMKLMFHLAILDTLFLGILASATSAAGAVGYIGYKGNSHTRWNEVCNTFGSFCGHVAGSISVSLLPSIALLLLVWLSIFEFSKKIRLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DH62</accession>
    <name>A0AAD5DH62_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">M8C21_016501</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">AA19_3_7</strain>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the splicing factor SR family. RSZ subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000097">
        <property type="protein sequence ID" value="KAI7757610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DH62"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000214">
        <property type="entry name" value="Serine/arginine-rich splicing factor 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050907">
        <property type="entry name" value="SRSF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23147">
        <property type="entry name" value="SERINE/ARGININE RICH SPLICING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RRM" evidence="13">
        <location>
            <begin position="92"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="14">
        <location>
            <begin position="203"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="23"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="171"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="215"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="32"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="180"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="230"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00061011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAI7757610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="277" mass="31323" checksum="4D883691D0A974DC" modified="2024-05-29" version="1">MDREDDDHAILTCEVDECINIEHTNDEINQQDQRDKPPNGSKDVDRHEEPDQREVNEVPKEEDRTPNEVVAHGKKETAAYDYVIEEVLKKQRSLTLGRVGHNKNTIQFVEFYFFLDPRVNERDIEDEFRTFGVIKSVWVARRPPGYGFVDFDDKRDALDAIRELDGKNGWRVEMSHNSRGSGGGGGGGGRGGGRGRSGGSDLKCYECGESGHFARECRGGGGGRRRSRSRSPAARYRRSPSYGRRSYSPRGRSPPRRNNYSRSPPYRGRDAVPYANG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G144</accession>
    <name>A0AAD5G144_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">No exine formation 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_015460</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012184">
        <property type="protein sequence ID" value="KAI7724599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G144"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35313">
        <property type="entry name" value="NO EXINE FORMATION 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35313:SF1">
        <property type="entry name" value="NO EXINE FORMATION 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="56"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="119"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="146"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="200"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="226"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="261"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="293"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="325"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="368"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="408"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="466"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="496"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="520"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="549"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="585"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="617"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="670"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="695"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="719"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="750"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="806"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="883"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="941"/>
            <end position="960"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="980"/>
            <end position="1000"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1034"/>
            <end position="1051"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="838"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="843"/>
            <end position="853"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7724599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1063" mass="117822" checksum="0E88121F2332AF55" modified="2024-05-29" version="1">MLPPDHHHPPSYHSFISPTTFSPNRLHTNGTTTTTSNPRPIKNRLNTSSFLHNPRIAFTLIPCAAFLLDLGGTPVVAALTLGLMIAYILDSLNFKPGSFFAFKWVQIEYPTVVVTLERILFSCLPFIGSVVFSWAIVSAVGMMNASYYYMVFNCVFYWLYSVPRVSSFKVRRQVSYHGGEVPDDSLILGQFESCLHTLNLLFFPLMFHVVSHSAILFSSASAVCDLFLLFFVPFLFLLYASTRGALWWVTKSEDQLRSIRVVNGAVALVVVVVCLEVRVVFHSFARYIQVPWPLNYLLVTVTMLGGAAGAGAYEMGMIADASSSLAFTGLAVFVSAAGAVVVGFPVLFIPLPAVAGFYLARFFTKKNLSSYFAFVVLGSLMVTWFVVHNFWDLNIWMAGMSLKSFCKLIVADVILAMAVPGLALLPQKLHYLTEVGLIGHALLLCHIENRFYTYSGIYYYSFDDEVMYPSYMVVLTTFVGLALVRRLSVDHRIGPKAIWVLTCLYSSKLVMLFMTSKTALWASAILLLAVSPPLLLYKDRSRAASKMKPWQGYSHAAVIALSVWFCRETIFEALQWWNGRSPSGGLLLGFCILLTGLACVPIVALHFSHVMSAKRSLVLVIATGILFIAMQPPVPSSWTYHSELIKAARQSSDDISIYGFITSKPTWPSWLLMSAILLSLAAVTSIIPIKYIVELRLLFSIAMGIALGVYISAEYFLQATVLHILIVTTMVCTSVFCVFTHLPSASSTKVLPWVFALLVALFPVTYLLEGQVRVKTILAESGVGDLGEEDSKLTALLAVEGARTSLLGLYAAIFMLIALEIKFELASLMREKFNEKGSGIRHSQSGQSSNSSSTFPPKMRFMQQRRVSTVPAFTIKRMAAEGAWMPAVGNVATIMCFAICLILNVNLTGGSNRAIFFLAPILLLLNQDSDFVAGFGDKQRYFPVTIVISGYLILTSLYSIWEEIWQGNVGWGMQVGGPNWIFAVKNLALMILTLPSHILFNQFVWSYKKRNDSMPLLTIPLNLPPGIISDVVKVRILGLLGIIYSLIQYLVSRQQYMSGLKYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G1S3</accession>
    <name>A0AAD5G1S3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_029713</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011975">
        <property type="protein sequence ID" value="KAI7725365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G1S3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000754">
        <property type="entry name" value="Ribosomal_uS9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00380">
        <property type="entry name" value="Ribosomal_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7725365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="125" mass="14796" checksum="C614CB915727E083" modified="2024-05-29" version="1">MQQQQQQQQHQNWLEMHEQVETQKTTEQDRLLEKTHPPIRCSQRANYVTIVIIKSFTIEWRINRCDLCVLAAGFLTRDSRIVERKKPGMAKARRSYQWYHIYHHPSGLGKALRGRKKKNKGLDAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G1Z2</accession>
    <name>A0AAD5G1Z2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_033267</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011895">
        <property type="protein sequence ID" value="KAI7725605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G1Z2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7725605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="159" mass="18346" checksum="2BF7D02D299261A6" modified="2024-05-29" version="1" fragment="single">SIGHSFDVRFFDSAKASVKLLLLDEANAMTNDFETVCHHIHATHEYHAFGSKHRTLRNSNDSVAEWCSWIDKSNPNTTRSQTSYHTLMQPYVQLERKCNKKDSEIMISKYLSKPWFSNGLDHIGVQTVINNEHLSEPIKQVNLLGYGTIIWCPQFEDFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G2R2</accession>
    <name>A0AAD5G2R2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_007058</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the major facilitator superfamily. Phosphate:H(+) symporter (TC 2.A.1.9) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011926">
        <property type="protein sequence ID" value="KAI7725516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G2R2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015098">
        <property type="term" value="F:molybdate ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17487">
        <property type="entry name" value="MFS_MFSD5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008509">
        <property type="entry name" value="MOT2/MFSD5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23516:SF16">
        <property type="entry name" value="MOLYBDATE-ANION TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23516">
        <property type="entry name" value="SAM (S-ADENOSYL METHIONINE) TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05631">
        <property type="entry name" value="MFS_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="74"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="128"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="170"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="201"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="285"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="318"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="349"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="390"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="412"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00044504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7725516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="461" mass="50666" checksum="85DC2F1F29FA5F64" modified="2024-05-29" version="1">MEVFYYMVFGGLGLVVGALELSKSNKDRITTSQSFTSFNNNYLLVYSLMMAGDWLQGPYVYYLYTTYGYGKGDIGQLFIAGFGSSMLFGTIVGSLADKQGRKRACITYCCTYILSCFTKHSPHYKVLLLGRVLGGVATSLLFSAFESWLVAEHNKRGFDQQWLSLTFSKAIFLGNGLVAIISGLFGNFLVDVATLGPVAPFDAAACILAIGMAIILSSWGENYGDPSDNKDLLSQFKAAAVAIASDEKIALLGAIQSLFEGSMYTFVFLWTPALSPNDEEIPHGFIFATFMLASMLGSSVAARLMAHTSPRVESYMQIVFLVSAASLLLPIVTNFFVSPSEVKGGGISLGSSIQLVGFCAFEGCVGIFWPSIMKMRSQYIPEEARSTIMNFFRIPLNIFVCIVLYNVNAFPITIMFGMCSIFLFVAAVLQRRLLVITDKSKLDDWTSMKERDMENEPLNDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G3L0</accession>
    <name>A0AAD5G3L0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Flavin-containing monooxygenase</fullName>
            <ecNumber evidence="6">1.-.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_033241</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the FMO family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.-.-.-" evidence="6"/>
    <dbReference type="EMBL" id="JAMZMK010011476">
        <property type="protein sequence ID" value="KAI7726942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G3L0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004499">
        <property type="term" value="F:N,N-dimethylaniline monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000169">
        <property type="entry name" value="Flavin-containing monooxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000403">
        <property type="entry name" value="Flavin-containing monooxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000960">
        <property type="entry name" value="Flavin_mOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020946">
        <property type="entry name" value="Flavin_mOase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050346">
        <property type="entry name" value="FMO-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23023">
        <property type="entry name" value="DIMETHYLANILINE MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00743">
        <property type="entry name" value="FMO-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000332">
        <property type="entry name" value="FMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0274">FAD</keyword>
    <keyword evidence="2 6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="5 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7726942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="515" mass="58553" checksum="283FFBD42C633A98" modified="2024-05-29" version="1">MDKKQVIIVGAGISGLLACKYCLSKGFNPTVFDLESDIGGVWVKTIKTTRLQAPKHLYLFSDFPWPDSVMDVFPTQQQMLEYIHSYANHFNLMPHIKLQSRVNSISYDGPSSQTWSLWNGTGETFPPIGKWNVSVTDTNTTTTQVYIADFVILCLGRFKDVPNIPEFPVGKGPENFQGKAIHSMEYAAMDNEEAAAFVKEKKVVVVGFGKTGLDIARECASINGPEHPCTIVYRRDHWKLPDWAPWGIPLTYLYFNRFSELLVHKPGEGFVLNLLATLLSPLRWGVSKFVESHIKNKLPLAKFDMVPQHSFSKDARSCLILYMPDPDNFFNAVEEKSIKLKKSQSFSFYEKGISTDEDNTQIEAEIVIFATGFKGVEKLKDVFESSTFGHFISGSPRVPLYRECIHPHIPQVAVIGFSESLSNMYISEMRAKWVATLLEGMFKLPSVCEMEKDIARWDEYMKQSTEEYHYRSSIGALEIWYNDQLCKDMGMNQLRKNGTFANLFEPHGPMDYVKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G6B9</accession>
    <name>A0AAD5G6B9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">phosphatidylinositol-3,5-bisphosphate 3-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.95</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_019998</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class myotubularin subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.95" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010010852">
        <property type="protein sequence ID" value="KAI7730047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G6B9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052629">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate 3-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004438">
        <property type="term" value="F:phosphatidylinositol-3-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14507">
        <property type="entry name" value="PTP-MTM-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004182">
        <property type="entry name" value="GRAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030564">
        <property type="entry name" value="Myotubularin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010569">
        <property type="entry name" value="Myotubularin-like_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10807">
        <property type="entry name" value="MYOTUBULARIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10807:SF8">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-3-PHOSPHATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06602">
        <property type="entry name" value="Myotub-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00568">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51339">
        <property type="entry name" value="PPASE_MYOTUBULARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myotubularin phosphatase" evidence="8">
        <location>
            <begin position="247"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="545"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="703"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="549"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphocysteine intermediate" evidence="4">
        <location>
            <position position="488"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="402"/>
            <end position="403"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="488"/>
            <end position="494"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR630564-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR630564-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7730047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="891" mass="99614" checksum="BF7A6BAB885C3526" modified="2024-05-29" version="1" fragment="single">EKHKLVGKSESLVLLSPLIPTTNRRGLTFINHHHYHQMATPRNRRTTSLRDSDTTPLPDSSNNIINIIEPTATWDALDWTNIHQPVSRPVVPHGLSDFLYEAEEVIAEGYGVLVNTDEAGTLYVTNFRLLFLSEGSRDIIALGTIPLATIERFNKIVIKQQSGPRQSDKTPTRRLLQVIGKDMRIIIFAFRPRSKQRRAIFEALLRYARPTRLWDLYAFSSGPSRFSNTSPRVRLVNEYLRLLGVESHHSSLSTIEDGSFTLSNNWWRISDVNATYEMCPTYPFSLLLPKSIRDEDVLQACKFRARCRLPVISWCDGRTGAVLARSSQPLVGLMMNMRSNADENLVGALCTELTGGKRGRRKLYIADARPRKNALANGAMGGGSESSSNYFQSEIVFFGIDNIHAMRESLVRLRDYLDTHGTKSSDGMSSFLRHGGWTWGGGNLSSMSASVSTLGDSGWLIHIQNVLAGSAWIAARVAIESASVLVHCSDGWDRTSQLVALASLLLDPYYRTIKGFQALVEKDWLAFGHPFSDRAGMASLTGSGLELPRQSSSTNFPSSPMRQTPGSFPSQSPMSNQTQNSNHYSPIFLQWVDCVSQLLRMYPFAFEFSSAYLVDFLDCTLSYRFGNFFEKERQQYGVFDSCGCLWMYLDDIRASGGSSHVHYNLFYDPSKYVGPLLPPAAALAPTLWPQFHLRWSCPSEAQAREVEAQCRNLVEKISELQRAKDVAESKARETIATMEAIKAEIRNEKQISSSALNLATKANRENDIIKRAIQSLGCKISVSGNDECTINIESNLVNLPEKITSSRSTRGIPDAYDEKMDMSISVALTSDDDDVSENPILRVCENLCPQRTRDGGCKWPDSSCAQLESQLVGLRANYDAFDQLSIYDSYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G6M6</accession>
    <name>A0AAD5G6M6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 22">Peroxidase</fullName>
            <ecNumber evidence="5 22">1.11.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">M8C21_018573</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="24">AA19_3_7</strain>
            <tissue evidence="24">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 22">
            <text>2 a phenolic donor + H2O2 = 2 a phenolic radical donor + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:56136"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:139520"/>
            <dbReference type="ChEBI" id="CHEBI:139521"/>
            <dbReference type="EC" id="1.11.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="19 22">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="19 22">Binds 2 calcium ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="19 22">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
        <text evidence="19 22">Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 22">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peroxidase family. Ascorbate peroxidase subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="22">Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.7" evidence="5 22"/>
    <dbReference type="EMBL" id="JAMZMK010010792">
        <property type="protein sequence ID" value="KAI7730362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G6M6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140825">
        <property type="term" value="F:lactoperoxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00693">
        <property type="entry name" value="secretory_peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.420.10:FF:000010">
        <property type="entry name" value="Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.520.10:FF:000006">
        <property type="entry name" value="Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.420.10">
        <property type="entry name" value="Peroxidase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002016">
        <property type="entry name" value="Haem_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000823">
        <property type="entry name" value="Peroxidase_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019794">
        <property type="entry name" value="Peroxidases_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019793">
        <property type="entry name" value="Peroxidases_heam-ligand_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033905">
        <property type="entry name" value="Secretory_peroxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31235">
        <property type="entry name" value="PEROXIDASE 25-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00141">
        <property type="entry name" value="peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00458">
        <property type="entry name" value="PEROXIDASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00461">
        <property type="entry name" value="PLPEROXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00435">
        <property type="entry name" value="PEROXIDASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00436">
        <property type="entry name" value="PEROXIDASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50873">
        <property type="entry name" value="PEROXIDASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 19 22" id="KW-0106">Calcium</keyword>
    <keyword evidence="14 21" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 22" id="KW-0349">Heme</keyword>
    <keyword evidence="16 22" id="KW-0376">Hydrogen peroxide</keyword>
    <keyword evidence="13 19 22" id="KW-0408">Iron</keyword>
    <keyword evidence="9 19 22" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 22" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7 22" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 22" id="KW-0964">Secreted</keyword>
    <keyword evidence="10 22" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="22">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041777830" description="Peroxidase" evidence="22">
        <location>
            <begin position="21"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="Plant heme peroxidase family profile" evidence="23">
        <location>
            <begin position="23"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="17">
        <location>
            <position position="64"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="19">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="20">
        <location>
            <position position="60"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="21">
        <location>
            <begin position="33"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="21">
        <location>
            <begin position="66"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="21">
        <location>
            <begin position="116"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="21">
        <location>
            <begin position="194"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR600823-5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="RuleBase" id="RU362060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="KAI7730362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="324" mass="35399" checksum="8D422077F77DF354" modified="2024-05-29" version="1">MKIVLLAMIILFDILIVVNSEGQLKVGFYNQVCPNAESVVTNIVRDAAKSDHRMAAALLRLHFHDCFVEGCDGSILVDVGNTSERGAFGHQGLRGFEIIEEAKAKLESICPGVVSCADIVAMAARDAVAFSHGPVYQVETGRKDGLVSNIDLASRMPDVTDSIQLLKQKFIEKGLNDKDLVILSAAHTIGSTACFFMEERLYNFATSGRPDPSINPRFLPELTSTCPKHGDIMVRLPMDHGSEDTFDIQILQNIRSGFAVLQSDAKLMDDPRTRGIVESYFGVLAPLTGPSFEADFVTSMIRMGRIGVKNGSNGNIRRVCKSFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G897</accession>
    <name>A0AAD5G897_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_022163</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010140">
        <property type="protein sequence ID" value="KAI7732835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G897"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7732835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="188" mass="21555" checksum="F2AF1D7DDC7FC9DB" modified="2024-05-29" version="1">MSNQPSPFANLKNLKIHPEKDLLEVRQHETVKMSAEVRGYLLDSSPSATYTMVSREELRAMYDTRLAQNLIKQLRRFLEKEKAGIETKMAKMHEQGKAPVDIDMSWKDLSTQIEKGKEKASVIISMLKDINQVLTSLPASNRATFQPSFSTLRAETDIVMKKITDCIKMECDENQRRLSLCFHEFATT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G8F1</accession>
    <name>A0AAD5G8F1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_024976</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010099">
        <property type="protein sequence ID" value="KAI7732985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G8F1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043874">
        <property type="term" value="F:acireductone synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019509">
        <property type="term" value="P:L-methionine salvage from methylthioadenosine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01629">
        <property type="entry name" value="HAD_EP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000088">
        <property type="entry name" value="Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023943">
        <property type="entry name" value="Enolase-ppase_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01691">
        <property type="entry name" value="enolase-ppase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01549">
        <property type="entry name" value="HAD-SF-IA-v1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20371">
        <property type="entry name" value="ENOLASE-PHOSPHATASE E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20371:SF1">
        <property type="entry name" value="ENOLASE-PHOSPHATASE E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01133">
        <property type="entry name" value="C1.5.4:_Enolase-phosphatase_Li"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00044">
        <property type="entry name" value="enolase-phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7732985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="300" mass="33290" checksum="5C08702265EB322C" modified="2024-05-29" version="1" fragment="single">MFVLSSNRSILFSPLPKPPKCSNPAHLFLKAECQLDLDSSARSYGPFRKPKRILDLRITNTALKARALGIYNEAGSTPRCVVLDIEGTTTPISFVTDVLFPYARDNVRMHLEETKIKEDLQKGVVGAVPVPSDDAGKEEVIAALVANVEEMIKADRKITSLKQLQGHIWRTGFEKNEIKGVVYDDVPQAFERWHASGVKVYIYSSGSRLAQRLIFGYTNYGDLRKYLCGFFDTRVGNKRETQSYIEISESLGVDKPSDILFVTDVIQEAVAAKAAGLEVIISVRPGNGPLPENHGFNTIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G9T7</accession>
    <name>A0AAD5G9T7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">M8C21_001894</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">AA19_3_7</strain>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010010175">
        <property type="protein sequence ID" value="KAI7732716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G9T7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000004">
        <property type="entry name" value="Calcium-dependent protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000086">
        <property type="entry name" value="calcium-dependent protein kinase SK5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13202">
        <property type="entry name" value="EF-hand_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="25"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="17">
        <location>
            <begin position="128"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="17">
        <location>
            <begin position="164"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="17">
        <location>
            <begin position="200"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="17">
        <location>
            <begin position="239"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAI7732716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="290" mass="32642" checksum="008959536EEE4C1C" modified="2024-05-29" version="1">MNRRGSANQAFYVIGHKTENIRDLYTLGQKLGQGQFGTTYLCTENSTGIDYACKSISKRKLISREDVEDVRREIQIMHHLSGHPHIVSIKGAYEDPLYVHIVMELCNGGELFDRIIEMGNYRESLSEEEIAGLKEMFKAMDTDNSGAITFDELKAGLRKFGSTLMDTEIQDLMDAADVDNNGTIDYGEFVAATLHLNKLEREEHLVAAFKYFDKDGSGYITVDEVQQACADHRITDFPVEDIIKEVDQDNDGRIDYGEFVAMMTKQNAGIGRRTMRNSVNISMRGSPRAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GA85</accession>
    <name>A0AAD5GA85_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PWI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_020223</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010142">
        <property type="protein sequence ID" value="KAI7732823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GA85"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048024">
        <property type="term" value="P:regulation of mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1390.10">
        <property type="entry name" value="PWI domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002483">
        <property type="entry name" value="PWI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036483">
        <property type="entry name" value="PWI_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052225">
        <property type="entry name" value="Ser/Arg_repetitive_matrix"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23148">
        <property type="entry name" value="SERINE/ARGININE REGULATED NUCLEAR MATRIX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23148:SF0">
        <property type="entry name" value="SERINE_ARGININE REPETITIVE MATRIX PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01480">
        <property type="entry name" value="PWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00311">
        <property type="entry name" value="PWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101233">
        <property type="entry name" value="PWI domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51025">
        <property type="entry name" value="PWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PWI" evidence="3">
        <location>
            <begin position="27"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="120"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="124"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="189"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="293"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="316"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="434"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="493"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="526"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="538"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="568"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="582"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="621"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="668"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="679"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="742"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="769"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="790"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="811"/>
            <end position="828"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7732823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="848" mass="97858" checksum="E19D6FA0C848B565" modified="2024-05-29" version="1">MSGGFFRGTTADQDTRFSNKHAKLLKSHKFPSELEHLVDMSKVKMDVMRPWIAHRVTELLGFEDEVLINFIYGLLEEKVVNGKEIQISLTGFMEKNTSKFMKELWTHLLSAQQNASGVPQQFLDAKEEETRKKQEDTDHINRELKKAKEKESREHGQERGKMDREAVDVKAANLETHSRRRTKFSSKWSADEKGTDETNGSKETARMSRSPRPADRSMSPPRGTRSRSISSKSFTDSRSHSRSRSLSTSPKALRRSVSVGRRPCSLSRRSSTPNRVGSRLRPPSPAPSHRRSPSPARCRLRSPSPMPSRGRSPSPARRRLRSPIRRRSRSPFWRRSRSPARRRSRSPLRRRSRSPIYRRSRTPIRRRSRSPVRRRSPSPIRRRSRSPIQRRSRSPAQRRSRTPVRRWSRTPIRRRSPTPIQRKSPSPFQRKPRSPYQQRSQSSASPYPVHSGSPPAPGRSPSPMRRRYQRAPSTPSRRSPPIRRRTTILMRQRSPSPRSRSSSPSGRISVSPTRGISPSYPKRGSPNRQQRSPVQSPRDGHRIVQRDAPDRHQASQKEIAERPISVRSLERDPKGRKPDLSPSPYNSPSSSVSPPDAGRSPSQERSLIPPKSQRESMSLKNRSDRVEVGEKTSSRVKEDRREIKNPVNDKNDKNKFSPEKFAGHHSSGDQVQKNNSKNDYGKRNYPEFLRDRTHDTEKGYKADENQETIKMSKVDRHNQSGLLDSGSDESDHKRKHKKSKRKDVTSDDDTSHDSHAEGRKEAKRRRKEEKRLRKEEKYKRREERRRKKDSLRSEKLKLKADKNVSPSSGFDKSHGSHDESTLSDKKKLEIELREKALESLRAKKGVGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GB99</accession>
    <name>A0AAD5GB99_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_010881</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cyclic nucleotide-gated cation channel (TC 1.A.1.5) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009446">
        <property type="protein sequence ID" value="KAI7735717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GB99"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034220">
        <property type="term" value="P:monoatomic ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000024">
        <property type="entry name" value="Cyclic nucleotide-gated ion channel 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45651">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-GATED ION CHANNEL 15-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45651:SF48">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-GATED ION CHANNEL 8 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0407">Ion channel</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="10">
        <location>
            <begin position="39"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7735717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="277" mass="31829" checksum="6F067B62F462347E" modified="2024-05-29" version="1" fragment="single">KWVETRGVDEESLVQSLPKDLRRDIKRHLCLNLVKRVPLFANMDERLLDAICERLKPSLYTESTYVLREGDPVDEMLFIIRGRLESVTTDGGRSGFFNRGFLKEGDFCGEELLTWALDPKSAGNLPPSTRTVKALTEVEAFALIADEVKFITSQFRRIHSRQVQHTFRFYSQQWRTWAASFIQAAWRRNSRRKLLELHRLEEKDEYDDDDDDGYVSNDDINEESALIRGRASSSSSLGATLHVSRFATNAMKQIHRKRGMSGLISLQKPSEPDFAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GC55</accession>
    <name>A0AAD5GC55_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_005418</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009150">
        <property type="protein sequence ID" value="KAI7737005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GC55"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7737005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="90" mass="10182" checksum="C51300D6EDBA3072" modified="2024-05-29" version="1">MSSSSIGRERLATLSEEPIDEDGDYKHHHSIWYTSSKKAQTWLKTYFSLPHKHKPNNYLNVLLSVLGCPLFPVSQLPPQSSFSQPRVAGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GC92</accession>
    <name>A0AAD5GC92_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">cAMP-regulated phosphoprotein 19-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_025476</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the endosulfine family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009633">
        <property type="protein sequence ID" value="KAI7734848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GC92"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004864">
        <property type="term" value="F:protein phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006760">
        <property type="entry name" value="Endosulphine"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10358:SF28">
        <property type="entry name" value="CAMP-REGULATED PHOSPHOPROTEIN 19-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10358">
        <property type="entry name" value="ENDOSULFINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04667">
        <property type="entry name" value="Endosulfine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="49"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU363120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7734848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="98" mass="10918" checksum="B8D3143A6E83B77D" modified="2024-05-29" version="1">MDETSKVTSQQEEEVKKKYGGIVPKKPPLISKDHERAYFDSADWALGKQGVEKPKGPLEALRPKLQPTQQQTRYRKSPCAPADGEDGNGRPATEDSHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GDF3</accession>
    <name>A0AAD5GDF3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_028134</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009298">
        <property type="protein sequence ID" value="KAI7736361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GDF3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7736361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="98" mass="11206" checksum="A39E3C90B22E177E" modified="2024-05-29" version="1">MMTRGLVRDSVDRPRAALELNQIKNLEHCLPYFNNPFKDDEIEQSTIVDIAYPIQGRPVVCSFDLIGSWISLCYEDPDMAGVTLWYNAQGMYTLPKCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GDG6</accession>
    <name>A0AAD5GDG6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Mitochondrial import receptor subunit TOM20</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_015645</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the translocation pore.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion outer membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Tom20 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009347">
        <property type="protein sequence ID" value="KAI7736168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GDG6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005742">
        <property type="term" value="C:mitochondrial outer membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045040">
        <property type="term" value="P:protein insertion into mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010547">
        <property type="entry name" value="TOM20_imprt_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32409">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM20-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32409:SF16">
        <property type="entry name" value="PLANT SPECIFIC MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06552">
        <property type="entry name" value="TOM20_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="181"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="146"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7736168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="208" mass="22753" checksum="6F25A983232BDAD5" modified="2024-05-29" version="1">MEMQNDFDRLLFFEHARRSAEVTYTKNPLDTENLTRWGGALLELSTFQSVEESKSMIKDAISKLDEALSIDPKKHDALWCMGNAQTSYAFLTPDKDEAKGYFDSAYTFFLQAVEEDPGNELYQKSLEVATKAPELHSEVHKQGFGQQAMGAGAGGGGPSTSSASSVKGPKAQKSSDLKYDIFGWVILAVSIVAWVGFAKSSAPSPPQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GE48</accession>
    <name>A0AAD5GE48_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NADP-dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_007344</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008692">
        <property type="protein sequence ID" value="KAI7738955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050791">
        <property type="entry name" value="Aldo-Keto_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43625">
        <property type="entry name" value="AFLATOXIN B1 ALDEHYDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43625:SF55">
        <property type="entry name" value="PERAKINE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="3">
        <location>
            <begin position="72"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7738955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="300" mass="32782" checksum="EBA703DBCACE74C5" modified="2024-05-29" version="1">MSSGVRRIKLGSEGLEVSAIGLGCMGMSGGYGPPKPEQDMINLIHHAINSGVTHLDTSDVYGPHTNEILIGKRLDVDYIDLYYVHRIDTKVPIEITMGELKKLVEEGKIKHIGLSEAGPATIRRAHAVHPITAVQLEWSLWTRDAEEEVIPTCRELGIGIVPFCPLGSGFFASGPKVMENLADNDFRKVLPRLQXXXXXXXXXXXXXXXXXXXXXXXCTPSQLALAWVLHQGDDVGPIPGTTKIENLNQNLGALAVKLTAQEIAELESLASFKGARMPEFILAHTYKNSDTPPLSSWKSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GEZ5</accession>
    <name>A0AAD5GEZ5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Poly(A) RNA polymerase mitochondrial-like central palm domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_027387</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008925">
        <property type="protein sequence ID" value="KAI7737921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GEZ5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031499">
        <property type="term" value="C:TRAMP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990817">
        <property type="term" value="F:poly(A) RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043634">
        <property type="term" value="P:polyadenylation-dependent ncRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031123">
        <property type="term" value="P:RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05402">
        <property type="entry name" value="NT_PAP_TUTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054708">
        <property type="entry name" value="MTPAP-like_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045862">
        <property type="entry name" value="Trf4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23092:SF48">
        <property type="entry name" value="NUCLEOTIDYLTRANSFERASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23092">
        <property type="entry name" value="POLY(A) RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22600">
        <property type="entry name" value="MTPAP-like_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81631">
        <property type="entry name" value="PAP/OAS1 substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Poly(A) RNA polymerase mitochondrial-like central palm" evidence="2">
        <location>
            <begin position="953"/>
            <end position="1102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="411"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="803"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="411"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="440"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="450"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="803"/>
            <end position="819"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7737921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="1266" mass="140612" checksum="F2B5439BF3923B5D" modified="2024-05-29" version="1">MMQSKRQLIDSLTSHISLYNSHSSSPSSSNPNPNPRSSILKWFASLTPQQRQSHLTIVDKPFTQLLIQLQSKLNTNGHGSFIILPDIPSDSSNPNIPSFCFRKSHGLLTRVSFFSHPHNIIRQSVYLFDSKEGENSGTCCFSCLDSVTVSCEFVENVEAFVGVMDGVTNGEFLRGEDGVVMGSGDWVELNWLKGMGYYSLEAFVVNRLELALRLAWLQVNVGKKRGVKLKEKSVCAAAGVAANVFWRKVGCVDWWRKLDDQVKKKVFRVVLGKAARSLTHDILYGRSSGMEDELQTSCVREKQPLSYNQETIDTLRGKDFESGMTLFIPPSSKLSSIAQTFNRLLVVCEISNMLLAFDNGQFNEEALFFSSLEAVGTISDRILRRLRGLFMVVSLDYTKLELLEDLNSTKSVKKSKSKSKSGSAKKGKPRDVKKPIPVQISENISSTSDKPTKDEVDKSDTQEMDIKRESASTSVEMVVLFENGDGVISTTVQSTSKKNKRARKKHKKDGINNQLKNIVSQTAPVKVSGNPDTEIITNGASVVDDNPIEVNVSPATMPTFNNDNIIGSQNSDLIVREHVEEMETQSLLTSKEKIEQNPVVVHDGGPVTFSGNPPNERPIASPQLSYVHQSGFVHTVGLCTHDNHWVREDDFAVSGLDYSQYFGGGVMYWDSYDHPVASFSRPPSLSSDDSSWAWQEADMNRTVDDMVAFSSSYSTNGLTSPSATSFCSPFDPLGYVLPSEVSGKVLHSSSTMKDLSEENVSGSLANFPGDIEAKTGDLFPYPILRPIIIPNIDRSREFKRSYEHKSPCVPPSRREEPRIKRPPSPVVLCVPPVPDSRKQRGFPTVRSGSSSPRHWGVKGWFHGEKKSLSARQLSQTLPGALLQDRLIAISQLTRDQDSHPDLSIPLQSRELLDCSTRKSSLPVMHGLLNEEIDSFCKQVAGVNLTRKPYINWAVKRVTRSLQVLWPRSRTNIFGSNATGLSLPTSDVDLVVCLPPVRNLEPIKEAGILEGRNGIKETCLQHAARYLANQEWVKNDSLKIVENTAIPIIMLVVEVPCDAIVSVDQEISTHSNGGKHTDVQSIRIDISFKSSSHTGLQTTELVKQLTEQFPAATPLALVLKQFLADRSLDQSYSGGLSSYCLILLITRFLQHEHHYGRSISQNFGGLLMDFFYFFGNVFDPRRMRISVQGSGVYIDRERGYSIDPIYIDDPLYPANNVGRNCFRIHQCIKAFADAYSTLEDQLDNCDSSNGSFSVKLLPLIIPSIAPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GFH3</accession>
    <name>A0AAD5GFH3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_005044</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008870">
        <property type="protein sequence ID" value="KAI7738148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GFH3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7738148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="83" mass="9766" checksum="37921B885F40973A" modified="2024-05-29" version="1" fragment="single">MEDVNENQRTLATPDSFEIQLIVVITRKTKREGERQKKISNTGGGGHLRCHDGRDLQQWCWYLRGWWHPWWKPVTATVFGDIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GG18</accession>
    <name>A0AAD5GG18_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_028037</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008533">
        <property type="protein sequence ID" value="KAI7740019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GG18"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14707">
        <property type="entry name" value="bZIP_plant_BZIP46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000036">
        <property type="entry name" value="ABSCISIC ACID-INSENSITIVE 5-like protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043452">
        <property type="entry name" value="BZIP46-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22952">
        <property type="entry name" value="CAMP-RESPONSE ELEMENT BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22952:SF175">
        <property type="entry name" value="PROTEIN ABSCISIC ACID-INSENSITIVE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BZIP" evidence="6">
        <location>
            <begin position="329"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="137"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="269"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="354"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="14"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="144"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="269"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7740019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="420" mass="45923" checksum="6F517B631700145C" modified="2024-05-29" version="1">MVVQNTELMSPGEVEVESPIESDEQQNRPQLGRQSSIYSLTLDEFQHTVNESGKNFGSMNMDEFLNSIWTAEELQAQAQAQTQVQPLARASASGQFLMADPNMIARQQSLSRQGSLALPGSLSRKTVDEVWSEIRRAHEHDNNRPSNENNNIINNNNNNSGSDRVHGSAAQRQPTYGEMTLEDFLVKAGVVREQNHPSALVVQPQAEQVAPFGLFNPNGNNRVVGPPPGSAHMVRPTTGGGPFSPGYPVGKRAGPYQPQPPPCYPGMVGNGGGDGGYGQGLGTGSPASPVSSDGITLSQLDSGNQYGMDLGGVRGGRNRIIDGPVEKVVERRQRRMIKNRESAARSRARKQAYTVELEAELKLLKEENAQLKQAMIFEEMRMKGVPKEDESYVVWFYAKIDQGMEARSSHTFVGRVFSTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GG33</accession>
    <name>A0AAD5GG33_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">F-box protein At1g78280</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_019779</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the JARID1 histone demethylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008237">
        <property type="protein sequence ID" value="KAI7741220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GG33"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.650:FF:000045">
        <property type="entry name" value="F-box protein At1g78280"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041667">
        <property type="entry name" value="Cupin_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050910">
        <property type="entry name" value="JMJD6_ArgDemeth/LysHydrox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12480">
        <property type="entry name" value="ARGININE DEMETHYLASE AND LYSYL-HYDROXYLASE JMJD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12480:SF35">
        <property type="entry name" value="TRANSCRIPTION FACTOR JUMONJI, JMJC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13621">
        <property type="entry name" value="Cupin_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="8">
        <location>
            <begin position="21"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="JmjC" evidence="9">
        <location>
            <begin position="224"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7741220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="912" mass="105084" checksum="F9E8E22FD923D66D" modified="2024-05-29" version="1">MELDSVSQSEIVSKDRRPDGLGDFIVLPDEIVCSILNLLSPPDVARLACASSVMYILCNEEPLWMNLCLSKENRELQYKGSWKRTTLHGLQVLDKCVDPCKKPLHFDGFNSLFLYKRFYRCYTRLDGFAFDQGNVERKTDLSLEEFVQQYDAQKPVLVPGLADTWEARKTWTPEQMLSKYGDTSFRISQRSSNKIAMKLKDYVFYMQEQHDEDPLYIFDDKFGEVSPDLLKDYSVPHLFPDDYFNVLDSDQRPPFRWLIIGPERSGASWHVDPALTSAWNTLLCGRKRWALYPPGRVPLGVTVHVNDEDGDVDIETPTSLQWWLDFYPLLADEDKPIECTQLPGETIYVPTGWWHCVLNLETTIAVTQNYVNSKNFEFVCLDMAPGYQHKGVCRAGLLALEDNAKISMEEDNMSSSELTQKKKKLKIDEHMDDLSFDKNVSDLEFSYDINFLKMFLDKNRDHYNSLWSTSNCIGQRELRDWLWKLWIGKPGLRDLIWKGACIALNTNKWSKCMEELCSFHNISLPADEENEQILMEYGLHPYSLSSMTSESGLKNHVPNILATGIVFLENGSYKIMPWDGKGVPDVITEHSTIDDIFSGTEYPFGIWSKKLFEYKKAGMPLEELVSLGGHSNIWPYIITKRCKGKIYAELRDKLLEEDVSNLASFLGKQLHDLHVLPVTPLNELSYLHIEKKLEMGHDNGTMDNSIQNINLSAASKVFFTILNRKKKDVSIRLTKWGSPIPSTLIEKVNEYLPKDFVELHNLFKDENGKFMEHNCCSWVHTDIMDDNIHLEQMKESGNGKEGSWRPSYIYDFSNMSLGDPILDIIPIHLDIFRGDVELLKKLLDSYKLPFGKRCSSDDGCKVRRISYLAMCYCMIHEENILGAIFGMSKELRTAKSWEEVEEAVWGDLNKYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GHX2</accession>
    <name>A0AAD5GHX2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_008891</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008107">
        <property type="protein sequence ID" value="KAI7741909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GHX2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35099:SF10">
        <property type="entry name" value="BZIP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35099">
        <property type="entry name" value="OS02G0182700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="29"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="119"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="54"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="67"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7741909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="197" mass="21869" checksum="BE76F41FE3AF6CCD" modified="2024-05-29" version="1">MNTDDNWLQSAFDDVTLVAQVILNLRHSSPSSLPKTWTTRQRRSRPPPPATVKKPSSTRDSPTTPLSLSGGAATTSVSGGCGVEETSRLILDGSDALRSKVIRANETTPTKRPRRKKTLAELKEDEIALLKERKQLKRQLASLQATCQKQRKENESLKKMKNEMKSQQVLVVSNCTEEQKNDSFVIPDLNIPAGEEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GIN3</accession>
    <name>A0AAD5GIN3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">M8C21_020793</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">AA19_3_7</strain>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010007572">
        <property type="protein sequence ID" value="KAI7744175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GIN3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000173">
        <property type="entry name" value="proline-rich receptor-like protein kinase PERK8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000212">
        <property type="entry name" value="Proline-rich receptor-like protein kinase PERK8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047117">
        <property type="entry name" value="PERK1-13-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47982:SF32">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47982">
        <property type="entry name" value="PROLINE-RICH RECEPTOR-LIKE PROTEIN KINASE PERK4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 14 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 15" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="28"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="17">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAI7744175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="299" mass="33188" checksum="684CA33E7B7FE113" modified="2024-05-29" version="1" fragment="single">MYSPDHGGVGNSRAWFSYEELSEATDGFSGSNLLGEGGFGCVYKGLLADGREVAVKQLKIGGSQGEREFRAEVEIISRVHHRHLVSLVGYCISEHQRLLVYQYVPNNTLHYHLHGEGRPVMDWATRIRVAAGAARGLAYLHEDCHPRIIHRDIKSSNILLDDNFDAQVADFGLAKLAMDSNTHVTTRVMGTFGYMAPEYASSGKLTEKSDVFSFGVVVLELITGRKPVDGSQPLGDESLVEWVGSKARPLLNQALENEDFEELVDPRLGKNYVQVEMFRMIEAAAACIRHLAPKRPRMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GJH3</accession>
    <name>A0AAD5GJH3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ras-related protein RABA2a</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_004976</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007982">
        <property type="protein sequence ID" value="KAI7742451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GJH3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01868">
        <property type="entry name" value="Rab11_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000067">
        <property type="entry name" value="ras-related protein RABA1f"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0636">Prenylation</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7742451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="214" mass="23550" checksum="7549BF7E4C55A520" modified="2024-05-29" version="1">MGRRADEEYDYLFKVVLIGDSGVGKSNLLSRFTRNEFCLESKSTIGVEFATRTLQVEGKTVKAQIWDTAGQERYRAITSAYYRGALGALLVYDVTKPTTFENVKRWLKELRDHADSNIVIMLIGNKTDLKHLRAVGTEDAQTFAETEGLSFIETSALEAVNVEKAFQTILGEIYKIVSKKALAAANSAASIKQGETLVVGAQEGNTKSNCCSSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GLW4</accession>
    <name>A0AAD5GLW4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mitochondrial fission protein ELM1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_018034</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007377">
        <property type="protein sequence ID" value="KAI7745046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GLW4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000266">
        <property type="term" value="P:mitochondrial fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009367">
        <property type="entry name" value="Elm1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33986:SF17">
        <property type="entry name" value="MITOCHONDRIAL FISSION PROTEIN ELM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33986">
        <property type="entry name" value="OS02G0535700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06258">
        <property type="entry name" value="Mito_fiss_Elm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7745046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="387" mass="43437" checksum="34370A03C50070FF" modified="2024-05-29" version="1">MHTTVRRAIIIGNGFAGAENQCYGLIRALGLSNYHSLYRVCRPKGGINKWLNWLPVSTHRRLDRLKRQLFDPQSVVSDQSDILEADAELIAAKASETCEKEGPLLVVASGRDTISVASSVKRLAPDHVFTIQIQHPRSHLSRFDLVISPHHDYYPLTPEGKKQIPWFLRGWITPRQPPRKNVVLTLGALHQVDSDVLKCAASAWRDELASLCKPLLVVNIGGPIRHCRYGADLARQLTASLMNVLPTCGSVRISFSRRTPKQACIVSEILVREFRNHPKVSIWDGQDPNPHMGHLAWADAFVITADSVSMLSEACSTGKPVYVIGAERCTWKFSYFQKCLQERGMVRPFTGDENIAKTWTYPPLQDTREAANHVIRALAKRGWKLQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GM15</accession>
    <name>A0AAD5GM15_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Style cell-cycle inhibitor 1-A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_003881</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007513">
        <property type="protein sequence ID" value="KAI7744523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GM15"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044688">
        <property type="entry name" value="SCI-1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34117">
        <property type="entry name" value="STYLE CELL-CYCLE INHIBITOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34117:SF1">
        <property type="entry name" value="STYLE CELL-CYCLE INHIBITOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="149"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="156"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7744523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="172" mass="20364" checksum="0C9E1EB9D23BD963" modified="2024-05-29" version="1">MGSDRKSKKRRSPSAASSDEDEGMNKKRRSSEPKTNNNNNNNKKKKDKDPHRKHKSRKSSEGKSEEKHKHKHKHHKRDHREITNFKEISDADYFLKNNEFATWLKDERDTFFSDLSSESARKLFTEFVKAWNKKKLDSRYYDGIVTGPRSSHNWKIKSDAKGRRSLESERDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GNM9</accession>
    <name>A0AAD5GNM9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Flavin-containing monooxygenase</fullName>
            <ecNumber evidence="7">1.-.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_018037</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the FMO family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.-.-.-" evidence="7"/>
    <dbReference type="EMBL" id="JAMZMK010006882">
        <property type="protein sequence ID" value="KAI7746876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GNM9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004499">
        <property type="term" value="F:N,N-dimethylaniline monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000099">
        <property type="entry name" value="Flavin-containing monooxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000960">
        <property type="entry name" value="Flavin_mOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020946">
        <property type="entry name" value="Flavin_mOase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050346">
        <property type="entry name" value="FMO-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23023">
        <property type="entry name" value="DIMETHYLANILINE MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00743">
        <property type="entry name" value="FMO-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000332">
        <property type="entry name" value="FMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00370">
        <property type="entry name" value="FMOXYGENASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0274">FAD</keyword>
    <keyword evidence="2 7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6 7" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="5 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7746876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="422" mass="48205" checksum="D17FC252E6D62417" modified="2024-05-29" version="1">MTNSLNVAVIGAGVSGLVTARELLRQNHRVTVFEKSNQIGGTWVYNDQVEVDNCLDLDPNRTIVHSSIYKSLRTNLPRPLMGFSDFSFEDKSYGDPRLFPGHEEVLRFLQDFAKEFGVGEVIRFNSEVVRAELRGGGFVVEWWTTEGGYEEECFDAVVVCNGHHTEPRVADNVPGIQEWSRKQIHSHNYRVPEPYQDQVVVVIGSGASAFDISREIATTAKEVHLSSRSSNVKVTKLDGYENIWQHMMIKRVYDDGTIEFQDGYSIVADAILHCTGYKFHFPFLRINDMVHVDDNRVGPLYKHVFLPQLAPRLAFVGLTFNNGVVFRLVELQAKWIALALSGKISLPSKDEMLAYIHKFYQEMDETGIPKRNTHSLSFQFEYVDWLADQVGLHVEDRLKKIVDNLFENWMARPKGYRDVLVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GP56</accession>
    <name>A0AAD5GP56_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_017180</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006244">
        <property type="protein sequence ID" value="KAI7749965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GP56"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47361">
        <property type="entry name" value="RING/U-BOX SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47361:SF4">
        <property type="entry name" value="RING_U-BOX SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="12"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="155"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="166"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="200"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7749965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="222" mass="25224" checksum="46B7EF61EF333504" modified="2024-05-29" version="1">MVSTDENLYGYCAICLNKIALQETALVKGCEHAYCVICILRWATYKKEPTCPQCKNPFEFLNVHRSLDGSIHDYMFEESVCLLLRASWFHPLSVESREVLDDHVVEDVYPYAYEEAYDDEDEDLDEVYLGRSSNINIRIGNRRWGNNGFVRAGKQEARPVRHQPHSQDSGAGSSSREPKKKNAAARDEAAVGRRAKRALKREAADKAAAEKHQQHLVRLGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GPD3</accession>
    <name>A0AAD5GPD3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Trehalose 6-phosphate phosphatase</fullName>
            <ecNumber evidence="8">3.1.3.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_014573</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Removes the phosphate from trehalose 6-phosphate to produce free trehalose. Trehalose accumulation in plant may improve abiotic stress tolerance.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>alpha,alpha-trehalose 6-phosphate + H2O = alpha,alpha-trehalose + phosphate</text>
            <dbReference type="Rhea" id="RHEA:23420"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16551"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58429"/>
            <dbReference type="EC" id="3.1.3.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 8">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 8">Glycan biosynthesis; trehalose biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 8">Belongs to the trehalose phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.12" evidence="8"/>
    <dbReference type="EMBL" id="JAMZMK010006159">
        <property type="protein sequence ID" value="KAI7750387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GPD3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004805">
        <property type="term" value="F:trehalose-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005992">
        <property type="term" value="P:trehalose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01627">
        <property type="entry name" value="HAD_TPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1020:FF:000004">
        <property type="entry name" value="Trehalose 6-phosphate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000073">
        <property type="entry name" value="Trehalose 6-phosphate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006379">
        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044651">
        <property type="entry name" value="OTSB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003337">
        <property type="entry name" value="Trehalose_PPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01484">
        <property type="entry name" value="HAD-SF-IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00685">
        <property type="entry name" value="T6PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43768">
        <property type="entry name" value="TREHALOSE 6-PHOSPHATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43768:SF32">
        <property type="entry name" value="TREHALOSE-PHOSPHATE PHOSPHATASE C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02358">
        <property type="entry name" value="Trehalose_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0346">Stress response</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7750387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="375" mass="42260" checksum="3085E1B6200610C3" modified="2024-05-29" version="1">MTNPSAVVSDAQSAITMAITAAVNTSSHYSPSVPNSPCNITMFSRNKFPKSIETAVSATGNHPGLGKNIAWVDVMRASSPTRNSSEDSENKKHWIMKHPSALGVFEEIMSAAKMKQIVIFLDYDGTLSPIVANPDQAYMDPKMREAVKNVAKYFPTAIVSGRCRAKVYNFVRLSELYYAGSHGMDIKGPSSRKHQKGNQNVLCQPAKEFLPMMAEVYEILMEKIKDIDGANVENNKFCLSVHYRCVDEHSWSDLFKRVESVLNDYPNLKLTQGRMVLEIRPTIKWDKGNAIEFLLESLGYANSKDIVPIYIGDDRTDEDAFKVLRKRGQGFGILVSKTPKETEAVYSLQEPFEVMHFLQRLVSWKRSILRSYQQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GPD8</accession>
    <name>A0AAD5GPD8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">HSF-type DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_013507</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Homotrimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the HSF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006535">
        <property type="protein sequence ID" value="KAI7748389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GPD8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000037">
        <property type="entry name" value="Heat stress transcription factor B-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000232">
        <property type="entry name" value="HSF_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015">
        <property type="entry name" value="HEAT SHOCK TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015:SF455">
        <property type="entry name" value="WINGED HELIX-TURN-HELIX DNA-BINDING DOMAIN, HEAT SHOCK TRANSCRIPTION FACTOR FAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00447">
        <property type="entry name" value="HSF_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00056">
        <property type="entry name" value="HSFDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00415">
        <property type="entry name" value="HSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00434">
        <property type="entry name" value="HSF_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0346">Stress response</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HSF-type DNA-binding" evidence="11">
        <location>
            <begin position="61"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="144"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="241"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="145"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="247"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7748389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="310" mass="33485" checksum="7064BA5ED91E7620" modified="2024-05-29" version="1">MERSGGESTGAETSSRSIPTPFLSKTYQLVDDKAIDDVISWNEDGSSFIVWNPIEFAKDLLPKYFKHNNFSSFVRQLNTYGFRKVVPDRWEFSNDCFRRGEKHLLCDIQRRKIGSTAPSSSPPPPAVAAAAVVTVAALSPAKRATTVSPSNSCEEQVLSSNSSQGITTNLSRERTVSSGSDADLIGENERLRRVNDLLNKELSQMKQLCNNIYLMMSNYATNSPTESNSLKPLDLLPLKRSSEEFSGDGGGGGSGTDGGGIEDEVDINPRLFGVPIGMKRAREGTSGSDGADLQLKPPGGDVKSEPLDGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GQC8</accession>
    <name>A0AAD5GQC8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_019587</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006597">
        <property type="protein sequence ID" value="KAI7748138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GQC8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000537">
        <property type="entry name" value="Putative receptor-like protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47974">
        <property type="entry name" value="OS07G0415500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47974:SF9">
        <property type="entry name" value="RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="34"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="103"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7748138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="451" mass="50663" checksum="9A5B42C708646C18" modified="2024-05-29" version="1">MEERTANIITIASITNLIVLIIVTRIIFHLTEPFFLILGASLAAILSIISFFFIRRRNQSRRQKLETIIASEGKELRIEYSFLRKVAGLPAKFRLKELEQATDNWKSLLGKGASASVFKGCLKDGTAVAVKRILHGEHRSGEKEFKSEIAAIASVQHVNLVRLSKWIYNNNNKTSNSGSSNNNNNSLGGCLTWSQRLKVAIDVAKALAYLHHDCRSKVLHLDVKPENILIDGNYNAIVSDFGISKLMKRDDSRIVTGMRAPEWLLGMGISEKSDVFSYGIVLLELIGGRRSVKAIDVDSEGNKIRRIFEYFPKIVTEKLKSGNIMEVVDKRLIDTCHIDENEVIKLVHVALWCIQDKPRRRPSMTEVVKWLEGRVAVDEPPETKMVVVDLLSIAKGDHGEEVGWHNRNKKRKPGVVARVASQLNGCLAPNMYSVESRSKSFTYSMSIISPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GRM6</accession>
    <name>A0AAD5GRM6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_024028</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006236">
        <property type="protein sequence ID" value="KAI7749981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GRM6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006927">
        <property type="entry name" value="DUF639"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31860">
        <property type="entry name" value="HEAT-INDUCIBLE TRANSCRIPTION REPRESSOR (DUF639)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31860:SF3">
        <property type="entry name" value="PROTEIN, PUTATIVE (DUF639)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04842">
        <property type="entry name" value="DUF639"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="551"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7749981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="551" mass="62552" checksum="A9B54A98DEE66633" modified="2024-05-29" version="1" fragment="single">DYKIRLNDLDSSFFKDKINIWVSKTTNFVNDLTSPLVKNVQDNNPTIKNANEIDDMDELLMTEHTIEAKTPMGNLSLAAIISIEQFSRMNGLTGKKMQMMFKALVPENVSNDARNLVEYCCFKFLCRDNSEIHPSLKDQAFQRLIFVTMLAWEHPYSYGKDSLGNIKVSFQREVVEEEAFVRIAPHISGLADWSTAHNLFKALAGGQNGISFHVWSTYIRQLLKVYEGQKSNVFEDSPLVSSGERILCLGSSRKRPVLKWENNMAWPGKLTLTDKALYFQEIGLRGQRDAIRLDLTGTGSRVEKARVGPLGSDLFDSAVSITSGPDSKKWILEFVDFGGEMRRDGKEKAISCAINSIARLQALQFVRKLLDEPTKLVQFSYLQNTPYGDIVRQTLAVNFWGGPLISGLKQVNSEASEVYNNHVYDIDRSVYLRRWMRHSSWNSNDSIAFWKNSLVRQDLVLSKNHVVGYNNLVEKAAIVCKDKYQVVESTQATIDAAMIEGIPSNIDLFKELVLPLIMAAKSFEKLRRWEEPHVTASFLAFIYAVIFRKAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GTI0</accession>
    <name>A0AAD5GTI0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Serine-threonine/tyrosine-protein kinase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_023909</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010002904">
        <property type="protein sequence ID" value="KAI7754630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GTI0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27001">
        <property type="entry name" value="OS01G0253100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27001:SF88">
        <property type="entry name" value="PROTEIN KINASE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine-threonine/tyrosine-protein kinase catalytic" evidence="3">
        <location>
            <begin position="2"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7754630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="74" mass="8466" checksum="913407733E5BF2EF" modified="2024-05-29" version="1">MEFAVEVEILARVRHKNLLSLRGYCAEGQERLIVYDYMPNLSLLSHLHGQHSAECLLDWNRRMNIAIGSAEGIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GTZ8</accession>
    <name>A0AAD5GTZ8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Replication protein A 32 kDa subunit B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_015067</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005706">
        <property type="protein sequence ID" value="KAI7752321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GTZ8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005662">
        <property type="term" value="C:DNA replication factor A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04478">
        <property type="entry name" value="RPA2_DBD_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000184">
        <property type="entry name" value="replication protein A 32 kDa subunit A-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040260">
        <property type="entry name" value="RFA2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014646">
        <property type="entry name" value="Rfa2/RPA32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014892">
        <property type="entry name" value="RPA_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13989">
        <property type="entry name" value="REPLICATION PROTEIN A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13989:SF16">
        <property type="entry name" value="REPLICATION PROTEIN A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08784">
        <property type="entry name" value="RPA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036949">
        <property type="entry name" value="RPA32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="6" id="KW-0234">DNA repair</keyword>
    <keyword evidence="2" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OB" evidence="8">
        <location>
            <begin position="73"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="Replication protein A C-terminal" evidence="9">
        <location>
            <begin position="168"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7752321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="250" mass="27614" checksum="A046AE81A604DE7E" modified="2024-05-29" version="1">MYGGDFDGNAAFSGGGFMPSQATQSTDPFPSALSKHRDTQTLIPLTVRQINKALLSNDDKVNILIDGVDVNNVKLVGMVMNKAERVTDVSFVLDDGTGRIDCNRWVHEAVDTKEMEAILERMYVRVHGQLKGFQGKKQLVVFGIKPVTDFDEITHHFVECIYVHSYNTKLMKQQPGSSNQTHMPSAAVTTQTYQTGPSNQFTGQHVADGLQGVEKMVLDYLHLPGSLARESGVHQEELVKQLGIPLNKIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GVZ8</accession>
    <name>A0AAD5GVZ8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Lactation elevated protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_022214</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AFG1 ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005269">
        <property type="protein sequence ID" value="KAI7753778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GVZ8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001040">
        <property type="entry name" value="AFG1-like ATPase isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005654">
        <property type="entry name" value="ATPase_AFG1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040713">
        <property type="entry name" value="ZapE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12169:SF6">
        <property type="entry name" value="AFG1-LIKE ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12169">
        <property type="entry name" value="ATPASE N2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03969">
        <property type="entry name" value="AFG1_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="65"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7753778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="604" mass="69382" checksum="D5F24D2089BA3499" modified="2024-05-29" version="1">MSRLIISSFRSLRFASRHQTSYLPFTALRLYSDHPQPSTPTNNPGPLAQYKNLVQEGKLQFDPYQEKVALELDNLVERLVQYEKEMEEYHVKLAKWEEDRENERRRLLLKEAEGKQQRSEVLDKTHNRFLGRWLDRKKPVNVETGVGKWVSYLNREKKLDLVVGKRPAVPPAPKGLYIYGNVGSGKTMLMDMFYSATKDIVTHRRRFHFHEAMLEINERMHKVWKNEVQEKSMQSSIANWIMNLPFDTKVKEWIAAEESYKENMQMKNILPAVADKFLVDQKSDRRGASILCFDEIQTVDVFAIVALSGIISRLLSTGTVLVATSNRAPSDLNQDGMQWEIFLKLLDKLEDHCETVLIGSETDYRRLLSQRSINLVTYFYPLNSIATKKFENVWDETIRKIGGPVRSETISVMFGRSLEVPESCNGVAKFTFEYLCGRPVGAADYIAVAKNYHTIFVSDIPMMSMRIRDKARRFIILIDELYNHHCCLYCSAASSIDELFQGTEEGTLFDLERQFETETEGSKLRRDVLAEGNIGSGGAPAGIISMLSGKEEMFAFRRAVSRLVEMQTPFYQEGVRLHHPLFQADRNSFNTPNIKHVTGLATNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GWA1</accession>
    <name>A0AAD5GWA1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_017373</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000032">
        <property type="protein sequence ID" value="KAI7758067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GWA1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35277">
        <property type="entry name" value="OS09G0363700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35277:SF10">
        <property type="entry name" value="OS09G0363700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="121"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="185"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7758067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="217" mass="24111" checksum="5BC00BB849645AA6" modified="2024-05-29" version="1">MDESKPDPSSDNMGSPSDLRETHGSVEDIDVDTPISEVKGPNIFHRAKEEIEAIVDTIQSKREPASPRKQSSLADNSGSPSHCRDNGKGEDIDADTPISEFKGPNIFYRAKEEIEAIVDSIQSKRESASPKKQSSLTDRGGSPSHRRETHGREDIDPDTPISEFKAPNIFHRAKEEIEAIVDTINSKRESDRDAESPKKDGFRASISKMINKSRKRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BKC1</accession>
    <name>A0AAD5BKC1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_002709</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012210">
        <property type="protein sequence ID" value="KAI7724484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BKC1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7724484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="31" mass="3802" checksum="207C614CA45450E1" modified="2024-05-29" version="1">MSLAFDDNEDYFEKDTEYIVVRMTFCFATWN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BNG1</accession>
    <name>A0AAD5BNG1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA sliding clamp PCNA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_025181</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand.</text>
    </comment>
    <comment type="function">
        <text evidence="7">This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processivity during elongation of the leading strand.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the PCNA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011625">
        <property type="protein sequence ID" value="KAI7726607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BNG1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043626">
        <property type="term" value="C:PCNA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030337">
        <property type="term" value="F:DNA polymerase processivity factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006272">
        <property type="term" value="P:leading strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006275">
        <property type="term" value="P:regulation of DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019985">
        <property type="term" value="P:translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00577">
        <property type="entry name" value="PCNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.150.10:FF:000006">
        <property type="entry name" value="Proliferating cell nuclear antigen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.150.10:FF:000008">
        <property type="entry name" value="Proliferating cell nuclear antigen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.70.10.10:FF:000001">
        <property type="entry name" value="Proliferating cell nuclear antigen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.70.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00317">
        <property type="entry name" value="DNApol_clamp_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046938">
        <property type="entry name" value="DNA_clamp_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000730">
        <property type="entry name" value="Pr_cel_nuc_antig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022649">
        <property type="entry name" value="Pr_cel_nuc_antig_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022659">
        <property type="entry name" value="Pr_cel_nuc_antig_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022648">
        <property type="entry name" value="Pr_cel_nuc_antig_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00590">
        <property type="entry name" value="pcna"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11352">
        <property type="entry name" value="PROLIFERATING CELL NUCLEAR ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11352:SF0">
        <property type="entry name" value="PROLIFERATING CELL NUCLEAR ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02747">
        <property type="entry name" value="PCNA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00705">
        <property type="entry name" value="PCNA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00339">
        <property type="entry name" value="PCNACYCLIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55979">
        <property type="entry name" value="DNA clamp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01251">
        <property type="entry name" value="PCNA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00293">
        <property type="entry name" value="PCNA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proliferating cell nuclear antigen PCNA N-terminal" evidence="9">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="Proliferating cell nuclear antigen PCNA C-terminal" evidence="10">
        <location>
            <begin position="127"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00053268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7726607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="264" mass="29328" checksum="1DBB66B81B9B27E1" modified="2024-05-29" version="1">MLELRLVQGSLLKKVMESIKDLVNDANFDCSATGFSLQAMDSSHVALVSLLLRAEGFEHYRCDRNISMGMNLNNMSKMLKCAGNDDIITLKADDGSDTVTFMFESPTQDKIADFEMKLMDIDSEHLGIPEAEYHAIVRMPSSEFARICKDLSSIGDTVVISVSKEGVKFSTRGDIGTANVVCRQNTTVDKPEEATVIEMNEPVSLTFALRYMNSFTKATPLSNTVTISLSSELPVVVEYKIAEMGYIRFYLAPKIEEDDEENKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BNQ3</accession>
    <name>A0AAD5BNQ3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_018354</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAD(P)-dependent epimerase/dehydratase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011666">
        <property type="protein sequence ID" value="KAI7726464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BNQ3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43574">
        <property type="entry name" value="EPIMERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7726464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="352" mass="37944" checksum="A254F6EF6908C982" modified="2024-05-29" version="1">MGLLSPVSISPATFFTFTLPTNFLHSSSSSSLSFFKFPNNNNNNFTSKHISSSSISSISSSMTFSSLTVHASSSSSSSSSSTGAADQVDGSSSANVGATDLLIVGPGVLGRIVAENWRKEYPGCQITGETVTTDHHDELIKMGINPTLKGVKRDHKFPYVIYCAPPSRSSDYAGDVGEAASMWNGEGSFLFTSSSAPYDRYDNGDCIEDSPVVPIGKSPRTDILLKAEQKVLEAGGCVVRGMHLYYLKQGTIEANPDHLVNLIHYEDAASLCVAILKKKLRGRLFLGCDNHPVSRQEVMDLVAKSGKFDNTFVGFKGTDGVLGKKLNNSRTREEIGWEPKYKSFAHCLGVAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BNS7</accession>
    <name>A0AAD5BNS7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_032958</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011534">
        <property type="protein sequence ID" value="KAI7726787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BNS7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7726787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="84" mass="8984" checksum="54B394E2F2E14EAC" modified="2024-05-29" version="1" fragment="single">EEVVVVVVRHPLPSPSTAASGCGYGAADVRRCLIRGFRWRCGFEDGGFEDGGVVVTGNHQWWRPLIQILGLRMVGYGADGGGVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BQG0</accession>
    <name>A0AAD5BQG0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_021183</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011340">
        <property type="protein sequence ID" value="KAI7727612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BQG0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48223:SF1">
        <property type="entry name" value="ABC TRANSMEMBRANE TYPE-1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48223">
        <property type="entry name" value="DEFECTIVE 2759, PUTATIVE ISOFORM 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="105"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7727612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="221" mass="25430" checksum="40D343604CA357DA" modified="2024-05-29" version="1">MAETTAGSQAIQLFKSWLTLLRTPYSQSEIPDGTLEEGVSSNEMVETNCKIQNSGRSVILKAVWASFMGLDPAIKIPAMIFIPLYLAVNMKYGPEVAKELTPLWIFGPLVVALYVKIVQGLCLLYVYSFKQSVKVVKNLPTYYNYVMKGKLKEAIYTRLWQPVVDFRNLGYKEIWKRVQEWVIDKYLDYIESIWPHYCKLIRADLRQEVGNPILNWSSSQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BQG8</accession>
    <name>A0AAD5BQG8_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_025167</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011625">
        <property type="protein sequence ID" value="KAI7726593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BQG8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26312">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26312:SF226">
        <property type="entry name" value="TETRATRICOPEPTIDE-LIKE HELICAL DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="308"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="308"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="349"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7726593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="449" mass="50077" checksum="8536877F6784E610" modified="2024-05-29" version="1" fragment="single">MKVTTGCFNWSHPSIPHSSSSSSSKRRSFTDVSFVCRQSSIFGTKLNRSHSYETLISSNTNKCSIKRILSSTFDSHYSSNEYPPDIHDELHELTVRFQLSDDDVTNSDDDVTNSDDDVIELHNSDQFVTSYANWFGDMMPASVEWKAKSLGLPFSLRMIQKKKQLEEGLREVGEYAYCSMKKAFSSMVFIIRELQSYTLQMREVLFVEDLQGILMRVQKEMNASFVWLFQQVFSHTPNLMVCVMILLANYSVYAMSNQVGVAVLLPSPTVETTVSIVGEQMDVKFDSLTVKTCSITLGGKTTSIGGINSGGGKYGPVSGGTDGDGKYDQQNEHQTIVPDGGSSSTLNPASTSEESVSGEVSEAEELGLWNSIVAEAETMQGMTGVDGLDSETMRRFVSPVTVKLEDEADTEVYLKREFIYQMGLLEEPDNPLMLANYAQFLYLFYHDHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BQH3</accession>
    <name>A0AAD5BQH3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_013148</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Iojap/RsfS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011326">
        <property type="protein sequence ID" value="KAI7727758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BQH3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090071">
        <property type="term" value="P:negative regulation of ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004394">
        <property type="entry name" value="Iojap/RsfS/C7orf30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21043">
        <property type="entry name" value="IOJAP SUPERFAMILY ORTHOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21043:SF2">
        <property type="entry name" value="PROTEIN IOJAP, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02410">
        <property type="entry name" value="RsfS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7727758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="156" mass="17716" checksum="370E882473710B9D" modified="2024-05-29" version="1" fragment="single">YRFLSYLFQATDSLNKMASTSLSASFQIRFSASSLISPHDLCHLSPHANGWKSSHTTESYKLGRTIQRSLRGSSVVSPMALAALEFRNRINDLAEKQYGKFATGDTKANSWTLLDFGDVVVHIFLPEQREIYNLEEFYANATPIELPFENEQRFGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BR06</accession>
    <name>A0AAD5BR06_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_025193</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011625">
        <property type="protein sequence ID" value="KAI7726619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BR06"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01868">
        <property type="entry name" value="Rab11_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000067">
        <property type="entry name" value="ras-related protein RABA1f"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0636">Prenylation</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7726619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="216" mass="23533" checksum="818510185CBB15E2" modified="2024-05-29" version="1">MASRIDHEYDYLFKIVLVGDSGVGKSNILSRFTRNEFSLESKSTIGVEFATRTLEVDGKTIKAQIWDTAGQERYRAITSAYYRGAVGALLVYDITKNSTFDSLPRWLHELREHADANIVLMLAGNKSDLNHYRAVSEQDGQSFAEKEGLLFLETSALESYNVEKAFQTVLTDIYGIVSKKALVADEATLGLPGKGTTIDVGGGGASGNTKKGCCSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BRP8</accession>
    <name>A0AAD5BRP8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Non-specific lipid-transfer protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_020914</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Plant non-specific lipid-transfer proteins transfer phospholipids as well as galactolipids across membranes. May play a role in wax or cutin deposition in the cell walls of expanding epidermal cells and certain secretory tissues.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the plant LTP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011244">
        <property type="protein sequence ID" value="KAI7728272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BRP8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01960">
        <property type="entry name" value="nsLTP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.110.10">
        <property type="entry name" value="Plant lipid-transfer and hydrophobic proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036312">
        <property type="entry name" value="Bifun_inhib/LTP/seed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016140">
        <property type="entry name" value="Bifunc_inhib/LTP/seed_store"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000528">
        <property type="entry name" value="Plant_nsLTP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33076">
        <property type="entry name" value="NON-SPECIFIC LIPID-TRANSFER PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00234">
        <property type="entry name" value="Tryp_alpha_amyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00382">
        <property type="entry name" value="LIPIDTRNSFER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00499">
        <property type="entry name" value="AAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47699">
        <property type="entry name" value="Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="2 4" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042199788" description="Non-specific lipid-transfer protein" evidence="5">
        <location>
            <begin position="21"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical" evidence="6">
        <location>
            <begin position="24"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7728272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="110" mass="11022" checksum="BB27C4A598930EAC" modified="2024-05-29" version="1">MMLVITIVSLLMLSVPSTNAAISCSTVIQDVRPCVSYLTSGNGMPPAACCSGAKALAAAVTSTADKRAACSCLQSAARSLNPKPALAQSLPGNCGISLGFTISANVDCTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BT13</accession>
    <name>A0AAD5BT13_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CRAL-TRIO domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_003430</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011339">
        <property type="protein sequence ID" value="KAI7727639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BT13"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008526">
        <property type="term" value="F:phosphatidylinositol transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011074">
        <property type="entry name" value="CRAL/TRIO_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052578">
        <property type="entry name" value="PI_Transfer_CRAL-TRIO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45824:SF6">
        <property type="entry name" value="F16L1.9 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45824">
        <property type="entry name" value="GH16843P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03765">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01100">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46938">
        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="2">
        <location>
            <begin position="91"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="306"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="312"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7727639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="352" mass="40540" checksum="34A664437CC4FAA6" modified="2024-05-29" version="1">MNPQQKKLNSNGPLTLEDQHKLIIEVREAINKNKSFEKMKENSPNEFSRYCSDASISRYLRARNWNVKKAVKMFEASLTWRMAYKPEEILWEHVAAEAETGKIYRSSCKDKNGRVVLVLTPRFQNSKSVRAQVKYLVYCMENAILNLPSDQEQMIWLIDFHGFNLSNISIKSTKETAFVLQEQYPERLGLAILYNPPKFFEPFYKVRSLPYFAGQQVLGPGQTGQCYDPECQIASTVVKPFLEPKTANKVKFVYSDDPNTKTIMENLFCMDELESAFGGKHEEHFDIKKYAEKMKEDDAKRAVLYGRESCPEPSSNINSEPNPDNEEATDTNSVKEVDEEEISMETRKTDDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BT48</accession>
    <name>A0AAD5BT48_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PPPDE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_021811</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DeSI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011335">
        <property type="protein sequence ID" value="KAI7727699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BT48"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0101005">
        <property type="term" value="F:deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1720.30">
        <property type="entry name" value="PPPDE domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008580">
        <property type="entry name" value="PPPDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042266">
        <property type="entry name" value="PPPDE_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12378">
        <property type="entry name" value="DESUMOYLATING ISOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12378:SF44">
        <property type="entry name" value="PPPDE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05903">
        <property type="entry name" value="Peptidase_C97"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01179">
        <property type="entry name" value="DUF862"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51858">
        <property type="entry name" value="PPPDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPPDE" evidence="4">
        <location>
            <begin position="2"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7727699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="233" mass="25675" checksum="66C2DB23E6F90D23" modified="2024-05-29" version="1">MTEVILHVYDITNTGSDNTNNTILHINRIFKDGIRLGGIFHSAVQVYGEDEWSFGFCEQGTGVFSCPAKMNPMYTYRESVILGKTSLSKSKVNQILRELSREWPGNCYDLLSKNCNHFCNDFCESLGVSKLPGWVNRFANAGDTAVEIAETTAMRFRQAKTEIVTASKVAYRFLAGITSNNNTQGSLDSPGGSNRSNPRFQQPAWFKNLVAAGARPSTSATLESADGQIQKPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BTJ4</accession>
    <name>A0AAD5BTJ4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Polyadenylate-binding protein</fullName>
            <shortName evidence="11">PABP</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">M8C21_010246</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">AA19_3_7</strain>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Binds the poly(A) tail of mRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the polyadenylate-binding protein type-1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011006">
        <property type="protein sequence ID" value="KAI7729408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BTJ4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12378">
        <property type="entry name" value="RRM1_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12379">
        <property type="entry name" value="RRM2_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12381">
        <property type="entry name" value="RRM4_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1900.10:FF:000003">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000003">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000217">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000239">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000555">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1900.10">
        <property type="entry name" value="c-terminal domain of poly(a) binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036053">
        <property type="entry name" value="PABP-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006515">
        <property type="entry name" value="PABP_1234"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002004">
        <property type="entry name" value="PABP_HYD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034364">
        <property type="entry name" value="PABP_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045305">
        <property type="entry name" value="RRM2_I_PABPs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01628">
        <property type="entry name" value="PABP-1234"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24012">
        <property type="entry name" value="RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00658">
        <property type="entry name" value="MLLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00517">
        <property type="entry name" value="PolyA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63570">
        <property type="entry name" value="PABC (PABP) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51309">
        <property type="entry name" value="PABC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0945">Host-virus interaction</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 10 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="RRM" evidence="13">
        <location>
            <begin position="24"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="13">
        <location>
            <begin position="112"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="13">
        <location>
            <begin position="203"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="13">
        <location>
            <begin position="306"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="domain" description="PABC" evidence="14">
        <location>
            <begin position="542"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="467"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="481"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="15">
        <location>
            <position position="632"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAI7729408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="632" mass="69327" checksum="E256A2FA07082DE0" modified="2024-05-29" version="1" fragment="single">MATQVQVQGQAQAVNSGGQQFVPTSLYVGDLEWNVTDSQLYDLFNQLGQVVSVRVCRDLSTRRSLGYGYVNYASPQDAARAIEVLNFTPLNGKPIRIMYSHRDPSVRKSGSGNIFIKNLDKAIDQKALHDTFSTFGNIISCKIATDSTGQSKGYGFVQYDSEEAAQKAIEKLNGMLLNDKQVYVGPFLRRQEREMAVDKSKFTNIYVKNLSESTTDEDLNKIFSEYGTVTSAIVMKDADGNSKCFGFVNFESADDAAKAVDALNGQKFDNKEWYVGKAQKKEEREQELKQRFEQSMKEAVDKSQGLNLYIKNLDDTIDDEKLKELFSSFGTITSCKVMRDPNGTSKGSGFVAFSTSEEASRALVEMNGKMVASKPLYVALAQRKEDRRARLQAQFSQMRPIAMAPTVAPRMPMYPPGGPGLGQQLFYGQQPTFIPPQPGFGYQQQLVPGMRPGGAPMPNFFMPMVQQGQRPGGRRATAPGPQSQQPVPLMQPQMMPRGRVYRYPQGRNAPDASMSGMGMVSVPYDMSGIVPLHDAAVSQPIPIGALASALANASPTEQRTMLGENLYPLVEQIEPESAAKVTGMLLEMDQTEVLHLLESPDALKAKVSEAMEVLRSVSQQQAAAGGAANQLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BTM5</accession>
    <name>A0AAD5BTM5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Endoglucanase</fullName>
            <ecNumber evidence="10">3.2.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_016067</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endohydrolysis of (1-&gt;4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 8 10">Belongs to the glycosyl hydrolase 9 (cellulase E) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.4" evidence="10"/>
    <dbReference type="EMBL" id="JAMZMK010011002">
        <property type="protein sequence ID" value="KAI7729453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BTM5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008810">
        <property type="term" value="F:cellulase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000020">
        <property type="entry name" value="Endoglucanase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001701">
        <property type="entry name" value="Glyco_hydro_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033126">
        <property type="entry name" value="Glyco_hydro_9_Asp/Glu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018221">
        <property type="entry name" value="Glyco_hydro_9_His_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22298">
        <property type="entry name" value="ENDO-1,4-BETA-GLUCANASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00759">
        <property type="entry name" value="Glyco_hydro_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00592">
        <property type="entry name" value="GH9_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00698">
        <property type="entry name" value="GH9_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8 10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4 10" id="KW-0136">Cellulose degradation</keyword>
    <keyword evidence="6 8 10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3 8 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 8 10" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="71"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 9" evidence="13">
        <location>
            <begin position="109"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="512"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="560"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="569"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7729453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="619" mass="68622" checksum="3288A0436ADB0516" modified="2024-05-29" version="1">MYGRDPWGGPLEINNADSATDDDRSRNLNDFDKAALSRPLDETQQSWLLGPGEQKKKKYVDLGCIIVSRKIFVWTVGLILAAGFIAGFVTLIVKTVPRHHKHPPPPDNYTIALGKALRFFNAQKSGRLPKHNNVSWRGNSCLNDGKGDKSGAVLKDLVGGYYDAGDAIKFHFPKAFAMTMLSWSVIEYSAKYEAAGELNHVKEIIKWGTDYFLKTFNNTADSISQIVAQVGKGDTSGGESDPNDHSCWMRPEDIDYERPVTECSSCSDLAAEMAAALASASIVFKDNKAYSKKLVHGAATLWKFARDQRGSYSTGADAATFYNSSMYWDEFVWGGTWMYYATGNQSYLYLASHPTLAKHAGAFWGGPDYGVLSWDNKLTGAQVLLTRLRLFLSPGYPYEQILQTFHNQTSIIMCSYLPYFSSFNRTKGGLIQLNHGRPQPLQYVVNAAFLATLYSDYLDAADTPGWYCGPNFYSTNELRKFAQTQIDYILGDNPRKMSYLVGYGKRYPKQVHHRGASIPKNRVKYSCTGGYKYRDAKKPNPNTIVGAMVAGPDRHDGFHDVRTNYNYTEPTLAGNAGLVAALVALSGDSTVAKIDKNTIFSAVPPMFPTPPPPPAPWKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BTP3</accession>
    <name>A0AAD5BTP3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Single-stranded DNA-binding protein, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_003708</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011005">
        <property type="protein sequence ID" value="KAI7729437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BTP3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042645">
        <property type="term" value="C:mitochondrial nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006264">
        <property type="term" value="P:mitochondrial DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000160">
        <property type="entry name" value="single-stranded DNA-binding protein, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000424">
        <property type="entry name" value="Primosome_PriB/ssb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011344">
        <property type="entry name" value="ssDNA-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00621">
        <property type="entry name" value="ssb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10302">
        <property type="entry name" value="SINGLE-STRANDED DNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10302:SF13">
        <property type="entry name" value="SINGLE-STRANDED DNA-BINDING PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00436">
        <property type="entry name" value="SSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50935">
        <property type="entry name" value="SSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7729437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="205" mass="22591" checksum="7F1EC6C1857B5E17" modified="2024-05-29" version="1">MMMMSSVGRKLTRSLRVSLPNAFTPSLGKLYSSSSSSSGLGDDDDDIIDEEFLPEKPELQVQGVDPRKGWGFRGVHKAIICGKVGQAPMQKILRNGKTVTIFTVGTGGMFDQRTTGAKDLPKPAQWHRIAVHNETLAAYAVQQLTKNSSVYVEGDIETRVYNDSINGEVKNIPEICIRRDGKIRLFKTGESISSISFDELREGLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BTU3</accession>
    <name>A0AAD5BTU3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Autophagy-related protein 13 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_017433</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011017">
        <property type="protein sequence ID" value="KAI7729352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BTU3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990316">
        <property type="term" value="C:Atg1/ULK1 kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034497">
        <property type="term" value="P:protein localization to phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.900.10">
        <property type="entry name" value="HORMA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040182">
        <property type="entry name" value="ATG13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018731">
        <property type="entry name" value="Atg13_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036570">
        <property type="entry name" value="HORMA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13430:SF15">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 13B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10033">
        <property type="entry name" value="ATG13"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0072">Autophagy</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Autophagy-related protein 13 N-terminal" evidence="3">
        <location>
            <begin position="16"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="Autophagy-related protein 13 N-terminal" evidence="3">
        <location>
            <begin position="109"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="457"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="515"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="477"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="524"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="549"/>
            <end position="559"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7729352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="588" mass="65087" checksum="1F22AA1C3390F503" modified="2024-05-29" version="1">MAVNPHTEPAKMEQIITEFFAKSLHIILESRCPFVSSHNYSGEQLHSPSSSASASSSSSSIRPRNKWFNLPLRDCPAALENIDFWRQSNLEPMVVDVILVQRPVEFGCDGNSEKVIERWIVRYESRKGCKEGALGNKRSNTVTSHTLYKKSILLLRSLYVTVRLLPCYKIFREINSSGQIRSFSLVHRVSSSMEALTRRDEGEMQQFVFTPVDTSCGKLCISLLYLPSVYDAASESSTPMSPQFITDYVGSPLADPLKRLPSASLPQYSPSSPFGRRHSWSYDTRMASSPSTLPSPSPTYSESRTLYSRYGSHRPPISPRYISLEPAHVHAKDIGYDEYLPSPNYSQSPSPSPPVGSNMLTALLRSDSAPVYIPASRLGDAPLSSNKTVLPFSPPLNVTRPSLSLVERSLSIPTTSAKVLSLREEETARPSGVKISSNSSLHKSSFRSFRRLSFEDSYDDSEFSGPFVVDDDDDTIDPGSRPGSSDRSGNLGTQHERVKSQDAAVGALVQMLKKAPPLQKDLNRSQYVSKTSRPEKSSNQIQDHPAHASSNMSSFSRMSGIRTTSDALEELKGYVDVKQVLLKQSSKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BU62</accession>
    <name>A0AAD5BU62_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein RADIALIS-like 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_020943</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010930">
        <property type="protein sequence ID" value="KAI7729738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BU62"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048262">
        <property type="term" value="P:determination of dorsal/ventral asymmetry"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009908">
        <property type="term" value="P:flower development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000154">
        <property type="entry name" value="Transcription factor SRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044636">
        <property type="entry name" value="RADIALIS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43952">
        <property type="entry name" value="MYB FAMILY TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43952:SF72">
        <property type="entry name" value="MYB-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Myb-like" evidence="5">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="6">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7729738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="85" mass="10101" checksum="7EC2B97CA7E397BB" modified="2024-05-29" version="1">MASWTPRQNKQFEDALAYYNKDSPDRWHKIARAVGGKSVEEVKRHYDILVRDIMRIEADQVPLPNYRDIGRNGRGYENDQRLLRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BUE0</accession>
    <name>A0AAD5BUE0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_007748</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010901">
        <property type="protein sequence ID" value="KAI7729858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BUE0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7729858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="134" mass="14489" checksum="EFE63006EDA53717" modified="2024-05-29" version="1">MEPDRELIWTSPLPASQDKNGIQGCSITRLRVTRAEHGGYGCSASSFSKGVKRGVGRLSTSDASCFEVVPISAAVADVVRAPPRVFLFVKQEWATIRSSNSIALFCTKKSQNAVQDGDRDRIFVQNLGQDNASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BUL1</accession>
    <name>A0AAD5BUL1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cystatin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_010861</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010885">
        <property type="protein sequence ID" value="KAI7729916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BUL1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004869">
        <property type="term" value="F:cysteine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00042">
        <property type="entry name" value="CY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000010">
        <property type="entry name" value="Cystatin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046350">
        <property type="entry name" value="Cystatin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47364">
        <property type="entry name" value="CYSTEINE PROTEINASE INHIBITOR 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47364:SF2">
        <property type="entry name" value="CYSTEINE PROTEINASE INHIBITOR 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16845">
        <property type="entry name" value="SQAPI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54403">
        <property type="entry name" value="Cystatin/monellin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0789">Thiol protease inhibitor</keyword>
    <feature type="domain" description="Cystatin" evidence="3">
        <location>
            <begin position="52"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7729916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="93" mass="10294" checksum="0397929BB56842EA" modified="2024-05-29" version="1" fragment="single">GNWLEIASPDDPAVIEVGEFAVDEHNKDTNRVTLAENGQKTIGNWLEIASPDDPMVIEIGKFAVEEHNKESNDTLKFDEVIKGDTQIVGGMNW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BV13</accession>
    <name>A0AAD5BV13_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Patellin-4-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_001444</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the patellin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010835">
        <property type="protein sequence ID" value="KAI7730156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BV13"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011074">
        <property type="entry name" value="CRAL/TRIO_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044834">
        <property type="entry name" value="PATL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056794">
        <property type="entry name" value="PATL1-6_C_GOLD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45932">
        <property type="entry name" value="PATELLIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45932:SF3">
        <property type="entry name" value="PATELLIN-4-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03765">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25099">
        <property type="entry name" value="GOLD_PATL1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01100">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46938">
        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50866">
        <property type="entry name" value="GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="242"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="CRAL-TRIO" evidence="12">
        <location>
            <begin position="145"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="GOLD" evidence="13">
        <location>
            <begin position="311"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="13"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7730156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="421" mass="49277" checksum="026CDE9ACF025932" modified="2024-05-29" version="1">MDEDKDPQSPNETFHDTIEGEIIPKHEEQEPQTLIVTELKRKKKKALIELRTRLEDSIINYTLIRENYKSNENLRDIELWGVPLLPSRGQERTDMILKKFLKAKDYNVHNALEMIGNTIMWREDFNVDNVFNEVFGHDLDNIGCIEGRDKAGHPLCYQMFEGFKDKHMLRNRLGNKEACDEFLRWRIKLMEGCIRKLDFKHDGSDSVIQIIDVKNIPRHFLNEIFQGSKKYFSLLQENYPGLIYRFVIVNVPMWFFAFYTLNMRLMTRKYKVMYVKPSAITETLLKFIEPEQLLAQYGGLGREGGEFSPDDKVLEQKLKGHATEHIEIVTPEVGMTVYWDVTVTGSDVSYKEEFVPEDDGSYNVLVQKGKQIGRMTSNSFHVNEPGKILISFVNPTSKSRKIFYRYKLKPFQPMYTFSIHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BVF9</accession>
    <name>A0AAD5BVF9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_022738</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010833">
        <property type="protein sequence ID" value="KAI7730185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BVF9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071277">
        <property type="term" value="P:cellular response to calcium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04048">
        <property type="entry name" value="C2A_Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000099">
        <property type="entry name" value="Copine 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045052">
        <property type="entry name" value="Copine"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10857:SF106">
        <property type="entry name" value="C2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10857">
        <property type="entry name" value="COPINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="1">
        <location>
            <begin position="27"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7730185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="156" mass="17413" checksum="353B3670E97E730E" modified="2024-05-29" version="1">MGNCFSGAGVHGNFAVGGASSNPDPNGRNDAVDNFFKNRGVNGLFYQIELSLSASNLRDRDVFSKSDPIAVVYIKGKDGSLQELGRTEVVLNSLNPQWITKIKVTYCFEMVQTLLFRVYDVDTQFHDPQTLNLDDQQYLGECICQLSQIVTNPKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BW45</accession>
    <name>A0AAD5BW45_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">diphosphoinositol-polyphosphate diphosphatase</fullName>
            <ecNumber evidence="1">3.6.1.52</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_016068</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>1,5-bis(diphospho)-1D-myo-inositol 2,3,4,6-tetrakisphosphate + H2O = 1-diphospho-1D-myo-inositol 2,3,4,5,6-pentakisphosphate + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:79699"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:74946"/>
            <dbReference type="ChEBI" id="CHEBI:77983"/>
            <dbReference type="EC" id="3.6.1.52"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:79700"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>3,5-bis(diphospho)-1D-myo-inositol 1,2,4,6-tetrakisphosphate + H2O = 3-diphospho-1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:56312"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:140372"/>
            <dbReference type="ChEBI" id="CHEBI:140374"/>
            <dbReference type="EC" id="3.6.1.52"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:56313"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>5-diphospho-1D-myo-inositol 1,2,3,4,6-pentakisphosphate + H2O = 1D-myo-inositol hexakisphosphate + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22384"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58130"/>
            <dbReference type="ChEBI" id="CHEBI:58628"/>
            <dbReference type="EC" id="3.6.1.52"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="4">
            <dbReference type="Rhea" id="RHEA:22385"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>6-diphospho-1D-myo-inositol pentakisphosphate + H2O = 1D-myo-inositol hexakisphosphate + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:79703"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58130"/>
            <dbReference type="ChEBI" id="CHEBI:230534"/>
            <dbReference type="EC" id="3.6.1.52"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:79704"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein-tyrosine phosphatase family. Atypical dual-specificity phosphatase Siw14-like subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.52" evidence="1"/>
    <dbReference type="EMBL" id="JAMZMK010011002">
        <property type="protein sequence ID" value="KAI7729454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BW45"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052847">
        <property type="term" value="F:inositol-1,5-bisdiphosphate-2,3,4,6-tetrakisphosphate 5-diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052845">
        <property type="term" value="F:inositol-5-diphosphate-1,2,3,4,6-pentakisphosphate diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000024">
        <property type="entry name" value="probable tyrosine-protein phosphatase At1g05000"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020428">
        <property type="entry name" value="PFA-DSPs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004861">
        <property type="entry name" value="Siw14-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31126">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31126:SF46">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE DSP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03162">
        <property type="entry name" value="Y_phosphatase2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01911">
        <property type="entry name" value="PFDSPHPHTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="8">
        <location>
            <begin position="39"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00044949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7729454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="218" mass="24970" checksum="4DA1BEC10F31735F" modified="2024-05-29" version="1">MILLMIMNNPNQPDSAAVEGGNNNNDDGAGDLVLRPPINFATVEDRIYRSGFPQVSDFPFLDTLGLRSIICLCVEPYPKENLEYLKSRNIQLFQFGIDGTKDANIPKSVITNALKVLIDVRNHPVLIHCKRGKHRTGCLVGCFRKLQNWCLSSVLEEYKVYAGEKSRESDLEFLEKYDVSYLRQCLHSIIYQYHGYGSKKRRLLYKEDVQQKPPIRSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BWZ9</accession>
    <name>A0AAD5BWZ9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_002559</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010483">
        <property type="protein sequence ID" value="KAI7731283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BWZ9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7731283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="70" mass="8361" checksum="C53ECD1CB98029F4" modified="2024-05-29" version="1">MQVWFKFNQQRLLMEVGLAAKREDVAAMDPVAVKTMFLNEDEFRFCRVLLATTLGFGQFLSRRWRFVTIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BYH3</accession>
    <name>A0AAD5BYH3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">GOST seven transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_012937</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010636">
        <property type="protein sequence ID" value="KAI7730789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BYH3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053937">
        <property type="entry name" value="GOST_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009637">
        <property type="entry name" value="GPR107/GPR108-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21229:SF55">
        <property type="entry name" value="EXPRESSED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21229">
        <property type="entry name" value="LUNG SEVEN TRANSMEMBRANE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06814">
        <property type="entry name" value="GOST_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="130"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="162"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="219"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="GOST seven transmembrane" evidence="7">
        <location>
            <begin position="206"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF06814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7730789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="275" mass="31409" checksum="4F65AF773972841D" modified="2024-05-29" version="1" fragment="single">MQSRTGLIEAIVFEVKDTERIGGNFLKNSSNVLCCNPKLSADRSCTVGEVIIQKDENNPDWPKRIQTFFDGNNQEAKMPTQSVEINKTGMYYLYFMYCDPQFKGTTISGKTVWRNADGYLPGKMVPLMTFYGFISLAYLILGLAWFLRLYSFGKCDHLTCGYYNMGNCFLVTAFGGGNGIWCGEARTRWHDLKSVDSWFCIFFCLNLGTIDDFSGRTKLYLVLPVAFLDTWFILWIFSSLSKTLEKLQMRRSIAKLDVYRKFTNALALFVLVSIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BZ14</accession>
    <name>A0AAD5BZ14_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_030813</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010513">
        <property type="protein sequence ID" value="KAI7731213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BZ14"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052059">
        <property type="entry name" value="CR_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47973">
        <property type="entry name" value="CYSTEINE-RICH RECEPTOR-LIKE PROTEIN KINASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="139"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7731213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="171" mass="19487" checksum="7810E37CBBA3F74E" modified="2024-05-29" version="1">MAPEYALRGHLTYKADVYSFGVLLLEVVAGKSNTKHHPAGEFICLVDWVVALKQKGSLMDLMDPRLGSDFNKKEALRMIQIALLCINKYPAHRPTMSEVVKMLEGHIKIKEQDDNLTASDDEFRLQQLKMKLEEIQSPYFDEPETLTNPSSSIRDLYPDSQASEKGYLRLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BZE3</accession>
    <name>A0AAD5BZE3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_016851</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010339">
        <property type="protein sequence ID" value="KAI7732014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BZE3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009873">
        <property type="term" value="P:ethylene-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00018">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.730.10:FF:000001">
        <property type="entry name" value="Ethylene-responsive transcription factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.730.10">
        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001471">
        <property type="entry name" value="AP2/ERF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044808">
        <property type="entry name" value="ERF_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31190">
        <property type="entry name" value="DNA-BINDING DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31190:SF499">
        <property type="entry name" value="ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR ERF105"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00847">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00367">
        <property type="entry name" value="ETHRSPELEMNT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00380">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51032">
        <property type="entry name" value="AP2_ERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0936">Ethylene signaling pathway</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0611">Plant defense</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="9">
        <location>
            <begin position="115"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7732014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="259" mass="28546" checksum="60143CB5574D8267" modified="2024-05-29" version="1">MTSPDESSALDLIRQHLLIDDSTFLQTYTILSDHPNPSLISSCTNTITQQPQTLSNQLDFATEPDPEPIIQPGSHENTKQVQNNAYNNLKPSETVTINPFQIPVMERSELVEKKHYRGVRQRPWGKFAAEIRDPSKKGARVWLGTYDTAVEAAKAYDKAAFKLRGNRAILNFPLEIGEGGETVMVTETVTVRSNGRKRAGARLVEGDDGGSRKEVKVEAVKTVVDDGGDDDGDGKFRGVFDVQQLPYASMDFASGCMVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C0B2</accession>
    <name>A0AAD5C0B2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_028724</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010161">
        <property type="protein sequence ID" value="KAI7732787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C0B2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7732787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="127" mass="14600" checksum="D8012399E1277268" modified="2024-05-29" version="1">MLFKAQSNQWWPSIVLFVGYCCKLLYNKANSIPSNLDVWHIYWLDLPQILSKEIGFKGDPSDEYAFSTFFPELIRAFRCISIVTSYDYVDSHRGSTTTLFFVRQVSVTTDGNAYEVVYSTSPTQQPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C376</accession>
    <name>A0AAD5C376_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ENT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_025081</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009694">
        <property type="protein sequence ID" value="KAI7734553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C376"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050832">
        <property type="term" value="P:defense response to fungus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20404">
        <property type="entry name" value="Tudor_Agenet_AtEML-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1240.40:FF:000005">
        <property type="entry name" value="ENT domain containing protein, expressed"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1240.40">
        <property type="entry name" value="ENT domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014002">
        <property type="entry name" value="Agenet_dom_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033485">
        <property type="entry name" value="EMSY-LIKE_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005491">
        <property type="entry name" value="ENT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036142">
        <property type="entry name" value="ENT_dom-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002999">
        <property type="entry name" value="Tudor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33432">
        <property type="entry name" value="PROTEIN EMSY-LIKE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33432:SF28">
        <property type="entry name" value="PROTEIN EMSY-LIKE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03735">
        <property type="entry name" value="ENT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00743">
        <property type="entry name" value="Agenet"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01191">
        <property type="entry name" value="ENT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00333">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158639">
        <property type="entry name" value="ENT-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51138">
        <property type="entry name" value="ENT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENT" evidence="4">
        <location>
            <begin position="53"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="ENT" evidence="4">
        <location>
            <begin position="472"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="125"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="302"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="545"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="724"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="826"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="125"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="155"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="201"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="322"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="545"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="574"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="726"/>
            <end position="755"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7734553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="849" mass="92604" checksum="B1779E65E114AD81" modified="2024-05-29" version="1">MDYEPYDSSGTDDDLPPSHQNRIPRGGHVAGNGRSAVMGAVQYPPRVYGETDMEAQIHLLEQEAYSSVLRAFKAQADAITWEKESLITELRKELRLSNEEHRELLLKVNSDDVIRRIREWRQSSGLQPGMVNTGQAVHDPLPSPSVSGTRKKQKVNSSGPSQSFGVPPPHVPLPTSSSARRGPVPGSKAKNKKSMMGGASSIKTQHPTSGSAGRTQLGNRVTSGALANVPAETSSFSSLIGRKVRTRWPDDNNFYEATITDYNHNEGLHALVYDMYTVNETWEWVNLSEISPEDIQWVEGEDPGIPHRGSGHGMSRPVGRDSGPGGGRGRGMTKGQTRKDFPPSQNGIGKKGVNEIQLLNTDTLIKEVERVFGSSHPDPLEIEKARKVLKEHEQALADAIARLADISDGESVLVILMFRESGAAKSVLGTDDDLPPSHQNRIPRGGHVAGNGRSAVMGAVQYPPRMYGETDMETQIHLLEQEAYSSVLRAFKAQADAITWEKEGLITELRKELRLSNEEHRELLLKVNSDDVIRRIREWRQSSGLQPGMVNTSQAVHDPLPSPSVSGTRKKQKMNSSGPSQSFGVPPQHVPLPTSSSARRGPVPGSKGKNKKSMMGGVSSIKPQHPPSGSTGRAQLGNRVAPGVHANGAAETSSFSTLIGRKVRTRWPDDNNFYEATITDYNPNEGRHALVYDMYTTNETWEWVNLSEISPEDIQWVEGEDAGIPQKGGYGGSGRGVGRPVGRDSGPGGGRGRGMTKGQTRKDFPPSQNGIGKKRLDDIQLLNTDTLIKEVERVFGSGHPDPLEMEKARKVLKEHEQALADAIARLADISDGDSDEGGRSMRGQPVDRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C402</accession>
    <name>A0AAD5C402_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_009095</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009671">
        <property type="protein sequence ID" value="KAI7734677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C402"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053772">
        <property type="entry name" value="At1g61320/At1g61330-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34145:SF28">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34145">
        <property type="entry name" value="OS02G0105600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="56"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7734677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="521" mass="60020" checksum="5EA011311F531FE7" modified="2024-05-29" version="1" fragment="single">IHIPKINYITDERVQKSWIPKPSILCKSNHFQFIYSVTVLLETLAAASGIGEMDRISHLPDFIIHHILSFFDTSNGPPAELVRMSVLSKNWFQLTASFPILDFDIRNFASRESFFKYVEYTTSRFCHQNVPAHAFKLITSVREPAELDIVNRCLELVLQKGVGELVIDLSDPLEFQYESATYRLPNTLLSVSMLTCCPGLKRFCVPRHQNLQNVRISYKTLVESIDIESPNLSRLWVADWVQRGAPQMNLASCKKLTHVSYFGHPLPNSNGFTDFLSNFPFIENLVLVTQYNSNNLKLSSNSLRTLVLRSNCDFEEIEFRTPNLVSFSYPLNHTLLWPVVWHSTHLKPCMQIYPDRCIDTPWFKKLRRFLDKENGFKVFNLFIHAIYSQKLLVLENLKAIGLPPYELEHVELQLEPHEESSDHTAFVEAVLWCCRPRSLTLRSSFPTTDFEEQSDLVKFAYMKLLEQEDQGHTKVHIVSPSSSKAQKHFKGLMCEGKAISFIKEEGILSVRTKILRIASED</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C4D0</accession>
    <name>A0AAD5C4D0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_009102</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009671">
        <property type="protein sequence ID" value="KAI7734684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C4D0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7734684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="76" mass="9123" checksum="CEA794A6CFFF3420" modified="2024-05-29" version="1" fragment="single">MRCYPDHSVDTFWLQKLRLFLDKENGFKAFNLYIHTDQEVIVSEKLKAIELPPYELEHIELHFEDQESSDHVAFVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C4U3</accession>
    <name>A0AAD5C4U3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_008331</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009492">
        <property type="protein sequence ID" value="KAI7735391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C4U3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26312:SF132">
        <property type="entry name" value="OS01G0855200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26312">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7735391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="471" mass="53190" checksum="6C7438E8DAE31C7C" modified="2024-05-29" version="1">MISKMVNRCLNVSQHSVPHSPSSLKTLASINLSPSAKRRSLTQLVVAYRPAMFGTKLILNKSEPSSKHQPIKHVVNAILDENFSNDKENHNDLKLELELESESDWHGDMLLASTERKANSVDLPFSLRIIKKKKRCHNQIKELAGESAHSSVKKAFSSMVFILRELQTFTLQTIHLPSYEDLQAILVRVQSDMNASFVWLFQQVFSQTPPLMVSVMILLANYSVHSISNNFTTTSALPNVSMESVMNSNTYTEYNHSSMKTFCMNSGERTTSVGGNNGGGRKFKQVASGNDGNDKFDQFDTFLLENLGRVRANEEAVLGHQMNGGLDREMMKRFVSPVTARIEAEDVEDYLRTEVLYQMGLSQEPDNPLLLANYAQFLYVVARDYDRAEDYFKRASMVEPKDAEALNKYASFLWNVRKDLWAAEETYLEAISADPNNSFYAANYAHFLWNTGGEDTCFPLDSLENTFSSDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C502</accession>
    <name>A0AAD5C502_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">chitinase</fullName>
            <ecNumber evidence="2">3.2.1.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_025056</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">This protein functions as a defense against chitin containing fungal pathogens.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1-&gt;4)-beta-linkages in chitin and chitodextrins.</text>
            <dbReference type="EC" id="3.2.1.14"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the glycosyl hydrolase 18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.14" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010009694">
        <property type="protein sequence ID" value="KAI7734528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C502"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008843">
        <property type="term" value="F:endochitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02877">
        <property type="entry name" value="GH18_hevamine_XipI_class_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000015">
        <property type="entry name" value="Acidic endochitinase SE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045321">
        <property type="entry name" value="Cts1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001579">
        <property type="entry name" value="Glyco_hydro_18_chit_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050542">
        <property type="entry name" value="Glycosyl_Hydrlase18_Chitinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45708:SF69">
        <property type="entry name" value="CHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45708">
        <property type="entry name" value="ENDOCHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01095">
        <property type="entry name" value="GH18_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-0146">Chitin degradation</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041985479" description="chitinase" evidence="12">
        <location>
            <begin position="20"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="GH18" evidence="13">
        <location>
            <begin position="20"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00059418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7734528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="292" mass="31316" checksum="DAEC5F0EC97B5AC0" modified="2024-05-29" version="1">MAIYSALLLLATLVMGSNATGISIYWGQNGNEGTLAQTCATGNYEYVNIAFLWTFGNGRTPRMDLAGHCDPYSNGCTKLSADIESCQGKGVKVMLSIGGGVGSYSLASKQDAQQVATYLWDNFLGGKSSSIRPLGNASLDGIDFDIESGGSQHWDDLARYLSAYSTQGKKVYLTAAPQCPYPDAWLGGALQTGLFDYVWVQFYNNPPCQYSANISNLEDSWKQWTSDIPAAKIFLGLPAASAAAGSGFIPVTDLVSKVLPVIKSSPNYGGVMLWSKYYDDQTGYSAAIKKDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C6X4</accession>
    <name>A0AAD5C6X4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Hexosyltransferase</fullName>
            <ecNumber evidence="5">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_033770</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1 5">Glycan metabolism; pectin biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Golgi apparatus membrane</location>
            <topology evidence="5">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the glycosyltransferase 8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="5"/>
    <dbReference type="EMBL" id="JAMZMK010009566">
        <property type="protein sequence ID" value="KAI7735111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C6X4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047262">
        <property type="term" value="F:polygalacturonate 4-alpha-galacturonosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06429">
        <property type="entry name" value="GT8_like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029993">
        <property type="entry name" value="GAUT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002495">
        <property type="entry name" value="Glyco_trans_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32116">
        <property type="entry name" value="GALACTURONOSYLTRANSFERASE 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32116:SF110">
        <property type="entry name" value="HEXOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01501">
        <property type="entry name" value="Glyco_transf_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="3 5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="38"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7735111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="525" mass="60245" checksum="E43A3694F439A400" modified="2024-05-29" version="1">MQLQFSPSMRSITISSSSNGFFDFMKVKIAARHISYRTLFHAVLILACFLPFVFIVTALVTLEDCLGRRLGPKLLGRGEDSGRLVNELYKTLNHVSSVQVPNDLKLPENFTQLVTEMKDNKYDANEFAVILKGMMERSEKEIRESKFAELMNKHFAASSVPKGIHCLSLRLTDEYSSNAHARRQLPSPEFLPVLSDNSYYHFILSTDNILAASVVVTSAVQSSLKPDRIVFHVITDKKTYAGMHSWFALNPNSPAIIEVKGVHQFDWLTRDNVPVLEAVENHYGVRNYYHGNHVAGANLNDITPRFFASKLQARSPKYISLLNHIRIYLPELFPNLDKVVFLDDDIVIQRDLSPLWEVDLRGKVNGAVETCKGDDTWVMSKRFRTYFNFSQPLISNNLDPDECAWAYGMNIFDLRAWRRTDIRKTYHTWLKENLKSNLTLWRLGTLPPALIAFRGHVQPIDPSWHMLGLGYQNNTSVENVKKAAVIHYNGQSKPWLEIGFEHLRPFWSKYVNYSNEFVKSCHILE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C8E8</accession>
    <name>A0AAD5C8E8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_012644</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010009194">
        <property type="protein sequence ID" value="KAI7736769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C8E8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd14016">
        <property type="entry name" value="STKc_CK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055900">
        <property type="entry name" value="DUF7477"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24289">
        <property type="entry name" value="DUF7477"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="110"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="93"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="24"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7736769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="593" mass="66185" checksum="AE45CF3948615AB8" modified="2024-05-29" version="1" fragment="single">MPELRSGVRRRPVVNKNRSNPAPATVRTRAAAAREAAAAETVVVVEEVKRQRKKNNNNKNKNKKCLGNKVIVGLKKEEEEVKEVVMADGSGGLSANKVTGQEEEGNTAPFPDRVQVGGSPLYKVFVGRRVSGGTERISGPGATEVALKFEHRNSKGCSYGPPYEWQVYNTLGGSHGVPKVHYKGKQGEYYVMVMDMLGPSLWDVWNSSGQSMSSEMVSCIAVEALSILDKMHARGYVHGDVKPENFLLGQPSTSQEKKLFLVDLGLATKWRESGNGQHVDYDQRPDMFRGTVRYASVHAHLGRTASRRDDLESLAYTLIFLHRGRLPWQGYQGDNKSFLVCKKKMSTSPEVLCCFCPAPVRQFLEIVVNMKFDEEPNYSKLISLFEGLIGPNPAIRPINTDGAQKIIFQVGQKRGRMNIDDEDDGQPTKKIRMGVPATQWISIYNARLPMKQRYHYNVADARLSQHVERGNADGLLISSVASCSNLWALIMDAGTGFTSQVYELSPFFLHKEWIMEQWEKNYYISSIAGANNGSSLVVMSKGTPYSQQSYKVSDSFPFKWINKKWREGFHVTSMATAGTRWGIVMSRNAGFSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C9Y9</accession>
    <name>A0AAD5C9Y9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_015204</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycan biosynthesis; sucrose metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycoside-pentoside-hexuronide (GPH) cation symporter transporter (TC 2.A.2.4) family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the major facilitator superfamily. Phosphate:H(+) symporter (TC 2.A.1.9) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008882">
        <property type="protein sequence ID" value="KAI7738046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C9Y9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008506">
        <property type="term" value="F:sucrose:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17313">
        <property type="entry name" value="MFS_SLC45_SUC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000366">
        <property type="entry name" value="Sucrose transport protein SUT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000182">
        <property type="entry name" value="Sucrose transporter SUC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19432:SF93">
        <property type="entry name" value="SUCROSE TRANSPORTER 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19432">
        <property type="entry name" value="SUGAR TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13347">
        <property type="entry name" value="MFS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="8" id="KW-0769">Symport</keyword>
    <keyword evidence="7 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="98"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="131"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="209"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="255"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="368"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="421"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="451"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="494"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="529"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="561"/>
            <end position="580"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7738046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="597" mass="64623" checksum="85BFE1E8A97DBE6A" modified="2024-05-29" version="1">MDTMSIRVPYKNLNHDNDIEIVKLEEDETNRHMESDQSNNMFPNGNASNSNAPTQNCSMFTLVLSCAVAAGVQFGWALQLSLLTPYIQTLGIGHAFSSFIWLCGPITGLVVQPCVGIWSDKCTSKYGRRRPFILLGSLMISLSVLIIGYSADIGYLIGDTKEHCSTFKGTRPRAAFVFILGFWMLDLANNTGPARALLADLAGPDQRNSANAIFCLWMAVGNILGFSSGSSGKWHSWFPFLTSRACCEACGNLKAAFLVAVVFLTFCTMVTLYFAKEVPLAPKQHLRLSDVDRLLEDRQAQQHGLDHTESKPRKNDHKVGNRFENVFEIDASEKKVDSTYKEDQVESFSDSPSAVLVNLLTSLRHLPLGMHSVLIVMALTWLSWFPFFLFDTDWMGREVYHGDPKGSASESRAYGEGVREGAFGLLLNSVVLGISSFLIEPMCRWIGSKFVWAISNFIVFACMAGTAIITVISLKESRGTDNAVGGNEAIKTASLVVFAILGLPLAITYSVPFSVTADLTADTGGGQGLAIGVLNLAIVIPQMIVSLGAGPWDALFGGGNVPAFVLASLSALAAGIIATLKLPTPSNSYTPIGFHVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CAI8</accession>
    <name>A0AAD5CAI8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">tRNA/rRNA methyltransferase SpoU type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_019204</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009159">
        <property type="protein sequence ID" value="KAI7736941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CAI8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002938">
        <property type="term" value="P:tRNA guanine ribose methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18092">
        <property type="entry name" value="SpoU-like_TrmH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1280.10:FF:000016">
        <property type="entry name" value="rRNA methylase-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02060">
        <property type="entry name" value="tRNA_methyltr_TrmH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001537">
        <property type="entry name" value="SpoU_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033671">
        <property type="entry name" value="TrmH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43453">
        <property type="entry name" value="RRNA METHYLASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43453:SF1">
        <property type="entry name" value="TRNA_RRNA METHYLTRANSFERASE SPOU TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00588">
        <property type="entry name" value="SpoU_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="alpha/beta knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="1" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="tRNA/rRNA methyltransferase SpoU type" evidence="7">
        <location>
            <begin position="179"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7736941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="374" mass="42257" checksum="6ACCB577C533386E" modified="2024-05-29" version="1" fragment="single">SQAPICNLSLSSSSQSPARHSHIEMAACKFLFRYSISFLKLSTLRIETCTPSLFFPGHRLLSHTSGFKLKSYQRPYASIPAAVSVQYPEEEEEDNETKQTSVRDLIWRNSNDDVSSFMKMERRLIIDPHSNSRFPYLDKFKTGDTFLTSREVLDAVEPSLMELRKERFRNVVNNRTYSVCLVVEGLSDAGNVSAVFRSADALGFQSVHVVSLDSRKRYREHRHVSMGAEKWLDIELWDTTEECFEVLKSRGYRIATTHLGMETVSVYDMDWSQPTAIVVGNEGRGISEEALKLSDMHCSIPMKGMVDSFNVSVASGILMHHAVCDRTTRLGAHGDLTSEEKQILLAEFSLRHSRSSISIAEEYAKRKIAASPKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CB31</accession>
    <name>A0AAD5CB31_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_006635</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the polyprenol kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008896">
        <property type="protein sequence ID" value="KAI7738008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CB31"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031969">
        <property type="term" value="C:chloroplast membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039606">
        <property type="entry name" value="Phytol/farnesol_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32523:SF7">
        <property type="entry name" value="FARNESOL KINASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32523">
        <property type="entry name" value="PHYTOL KINASE 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0934">Plastid</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="46"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="68"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="112"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="135"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="168"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="194"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7738008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="242" mass="26382" checksum="54B1C0C9D2154355" modified="2024-05-29" version="1">MLFPESPVGGNLCALVLSTCLALSLLRLFEETARRGLFHQKLNRKLVHITIGLAFMFCWPLFSSGSGGAFVASLVPGVNIVKVLLIGLGILKDEATVKSMTRFGDYRELVKGPLYYASTITFCCIIYWRTSPISIAAICNLCAGDGFADIIGRRFGKKKLPYNKDKSFAGSIAMAIFGFMSSVGFMYYYSMFGFVERSFGMVIGFLVVSVASALVESHPLSTKLDDNLTVPLASVLIGIMVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CBP1</accession>
    <name>A0AAD5CBP1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Amine oxidase</fullName>
            <ecNumber evidence="1">1.4.3.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_028709</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-topaquinone</name>
            <dbReference type="ChEBI" id="CHEBI:79027"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
        <text evidence="1">Contains 1 topaquinone per subunit.</text>
    </comment>
    <comment type="PTM">
        <text evidence="1">Topaquinone (TPQ) is generated by copper-dependent autoxidation of a specific tyrosyl residue.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the copper/topaquinone oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.4.3.-" evidence="1"/>
    <dbReference type="EMBL" id="JAMZMK010008746">
        <property type="protein sequence ID" value="KAI7738712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CBP1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008131">
        <property type="term" value="F:primary methylamine oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009308">
        <property type="term" value="P:amine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.20">
        <property type="entry name" value="Copper amine oxidase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000269">
        <property type="entry name" value="Cu_amine_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015798">
        <property type="entry name" value="Cu_amine_oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036460">
        <property type="entry name" value="Cu_amine_oxidase_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10638:SF18">
        <property type="entry name" value="AMINE OXIDASE [COPPER-CONTAINING] ZETA, PEROXISOMAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10638">
        <property type="entry name" value="COPPER AMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01179">
        <property type="entry name" value="Cu_amine_oxid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49998">
        <property type="entry name" value="Amine oxidase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0186">Copper</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0801">TPQ</keyword>
    <feature type="domain" description="Copper amine oxidase catalytic" evidence="2">
        <location>
            <begin position="36"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7738712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="211" mass="24163" checksum="31C2AD25857EFC30" modified="2024-05-29" version="1">MIRGCGLISDQEALDYDLGASNLGKRLGETRDKIVNEDHGILWKHQAWRTGLADVRRSRRPTVSFICTVANCEYGFYWHFYQEDLQEKQQQYKKWKFVLHRTGKAVAGGCSYRAGSPNGSCRPLCSMFLLFVVEVDVKVEEPGKDSVHNNAFCTQETLLKSESHAMRDCNPLSARHWIVCVLDHSCSYVRRLTCDAIGEDWIYASGMFERK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CC45</accession>
    <name>A0AAD5CC45_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein DETOXIFICATION</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Multidrug and toxic compound extrusion protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_021957</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008762">
        <property type="protein sequence ID" value="KAI7738660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CC45"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13132">
        <property type="entry name" value="MATE_eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045069">
        <property type="entry name" value="MATE_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11206">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="142"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="175"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="205"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="233"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="280"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="319"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="361"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="435"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="461"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="22"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="22"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7738660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="561" mass="60974" checksum="F712B2A8428BEC2E" modified="2024-05-29" version="1">MCNPTQSSPSKVVVYKNTLFKNPNSNINPPKSMDNNNNNNHHQLHGWPTPSQALEEIKAIGKISGPTALTGLLLYSRAMISMLFLGYLGELELAGGSLSIGFANITGYSIISGLAMGMEPICGQAFGAKQMKLLGLTLQRTVLLLLSTSLPITFIWLNMKTILIWCGQDKEISNVAQTFIVFSIPDLFVLSLLHPLRIYLRTQNITLPLTCCSAVSILIHVPLNFLLVGYFKLGIIGVAVAVVITNLNLLFLLATFVYFSGIHRESWVPPSTDCLNGWSSLLALAIPTCVSVCLEWWWYELMILLCGLLSNPRATVASMGILIQTTSLVYVFPSALSLGVSTRVGNELGAKRPAKARISMIVSLVCAIAMGFIAMVFTTLMRHHWGRFFTTDVEILELTSVALPVVGLCELGNCPQTTGCGVLRGSARPTIGANINLGSFYFVGMPVAILMGFAVKLGFAGLWFGLFAAQVTCALLMLYVLFTTDWIVQVERANELAKPSSSSSTFSCLLPNSPLPTTIKNELKNSIEANLEEIKLISSDKEVELVKADCSSLETNPLIVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CC68</accession>
    <name>A0AAD5CC68_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Reticulon-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_026628</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Endoplasmic reticulum membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008639">
        <property type="protein sequence ID" value="KAI7739293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CC68"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009617">
        <property type="term" value="P:response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003388">
        <property type="entry name" value="Reticulon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045064">
        <property type="entry name" value="Reticulon-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10994">
        <property type="entry name" value="RETICULON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10994:SF65">
        <property type="entry name" value="RETICULON-LIKE PROTEIN B12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02453">
        <property type="entry name" value="Reticulon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50845">
        <property type="entry name" value="RETICULON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="34"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="128"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="Reticulon" evidence="7">
        <location>
            <begin position="24"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7739293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="154" mass="17403" checksum="6150A662E0D08936" modified="2024-05-29" version="1" fragment="single">MTSSDRLLFNRQATVHHLLGAGLFADVILWRDKNVTITILIITLASWMMFEKSDYTLLSYVSNVLLLLLTILFLWAKSAQILNRPTPPIPHLQLSEETVSEVATIIRDRTNMLLSVSYDIALGKDPKMFLKVAAYLVLISVIGSLTDFRTLCYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CCI1</accession>
    <name>A0AAD5CCI1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_013200</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008757">
        <property type="protein sequence ID" value="KAI7738665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CCI1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36245">
        <property type="entry name" value="GLYCINE-RICH PROTEIN DOT1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36245:SF5">
        <property type="entry name" value="GLYCINE-RICH PROTEIN DOT1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041996588" evidence="3">
        <location>
            <begin position="24"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="108"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7738665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="127" mass="13499" checksum="2F3671BE7DE1F5D8" modified="2024-05-29" version="1">MGAMKLMLLLLILLTFTSPLSFAHVVNYSGMNDNGVVNQSKFTHPGARGGGGRSTGENNNGIGGDSSSYRQGGGGAVIPVYAASGAAAKPNNHNNAANTCSMCWKHRFLIVISSFLSYFYYIYVVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CCL7</accession>
    <name>A0AAD5CCL7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">M8C21_030531</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">AA19_3_7</strain>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010008687">
        <property type="protein sequence ID" value="KAI7738998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CCL7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000004">
        <property type="entry name" value="Calcium-dependent protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000050">
        <property type="entry name" value="Calcium-dependent protein kinase 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="40"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="16">
        <location>
            <begin position="262"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="16">
        <location>
            <begin position="299"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="16">
        <location>
            <begin position="335"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="16">
        <location>
            <begin position="371"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="17">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAI7738998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="437" mass="50692" checksum="073BC9CDEC4DF535" modified="2024-05-29" version="1" fragment="single">NNIRRSRKQRKRIKHRTPKPFTRPIRVIKGTNGKDIYQHYKIGKELGRGEFGVSYECQDITTGERVACKKISKSKLRAQIDVEDVRREVSIMRHMPLHPNIVTFKDVYEDKDAIYLVMEICEGGELFDRIVAKGHYTERAAALVIKTILEVVQCPRECNNMDPKTEEGIAQAIIQGDINFIRDPWQRVSEDAKKLVKGMLDQDPYDRLTVEEALENHWIQNAHKVSIIPLGENVRSKIQQFSLMNKFKKKVITVVAENLPDEQVDGLKQMFDEMDKDKNGMLTFEEFKDGLCSIGDQPLDDHDVRMLMDAADLDENGVLNCEEFMMVAIHLKRISNDDHLRQAFHHFDKNKNGYIEFDELKESLFEEQNSHNEKLVNDIIHDADLDKDGRISYPEFAAMMTTGMDWKMASRQYSRVKLNAISVKMFKDQSKIKTIIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-10-08" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CCU4</accession>
    <name>A0AAD5CCU4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">AP2/ERF and B3 domain-containing transcription factor RAV1-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_006262</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AP2/ERF transcription factor family. RAV subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008719">
        <property type="protein sequence ID" value="KAI7738815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009873">
        <property type="term" value="P:ethylene-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00018">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10017">
        <property type="entry name" value="B3_DNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.730.10:FF:000008">
        <property type="entry name" value="AP2 domain-containing protein RAP2.8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.730.10">
        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.330.10">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001471">
        <property type="entry name" value="AP2/ERF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003340">
        <property type="entry name" value="B3_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015300">
        <property type="entry name" value="DNA-bd_pseudobarrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044800">
        <property type="entry name" value="LEC2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31140">
        <property type="entry name" value="B3 DOMAIN-CONTAINING TRANSCRIPTION FACTOR ABI3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31140:SF112">
        <property type="entry name" value="ETHYLENE RESPONSE DNA BINDING FACTOR 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00847">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02362">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00380">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01019">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101936">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51032">
        <property type="entry name" value="AP2_ERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50863">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0936">Ethylene signaling pathway</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="10">
        <location>
            <begin position="55"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="TF-B3" evidence="9">
        <location>
            <begin position="181"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="146"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="156"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7738815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="342" mass="38771" checksum="7B7AD4FF5D63C873" modified="2024-05-29" version="1">MDRSCIHDQTTTSEMTTRQMEISLCRVGSGSSVVXXXPDGGVEVEAQSRKLPSSRYKGVVPQPNGRWGAQIYEKHQRVWLGTFNEEDEAAKAYDIAVQRFRGRDAVTNIKHVVADDKEAAMEASFLSSHSKSEIVDMLRKHTYNDELEQSKRTRTSHQTLSRTGLTNSPGSGSIKPREHLFQKIVTPSDVGKLNRLVIPKQHAEKHFPVQKGSNSKGVLLHFEDNGSKVWRFRYSYWNSSQSYVLTKGWSRFVKEKNLKAGDSVSFHSTTGPDKQFYIDWKSKGPTQSIQPVKLVEPVQMLRLFGVDISSVISNNDQHERGKSEKELLGFRDCKKQRIIDVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CDC7</accession>
    <name>A0AAD5CDC7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_020873</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008558">
        <property type="protein sequence ID" value="KAI7739862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CDC7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7739862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="48" mass="5442" checksum="AB5107DCA75AE99B" modified="2024-05-29" version="1" fragment="single">MILLLRVKDSLRKTLHQRCSSDQIHCSLKNTKTKTNTNSSLTCSKDHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CDJ0</accession>
    <name>A0AAD5CDJ0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Outer envelope protein 61</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_004999</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008626">
        <property type="protein sequence ID" value="KAI7739454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CDJ0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053319">
        <property type="entry name" value="OEP61"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48433">
        <property type="entry name" value="OUTER ENVELOPE PROTEIN 61-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48433:SF1">
        <property type="entry name" value="OUTER ENVELOPE PROTEIN 61-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="536"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="180"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="227"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="393"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="393"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="420"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="433"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7739454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="558" mass="61944" checksum="4225D692114417DF" modified="2024-05-29" version="1">MFPGMMDPEMMRLAQDQMSRMSPADLARIQQQMMSNPDLVRMATDSMKHMKPEDLKYAAEQLKSTRPDEMAQIGEKMANATPEELAAMRSRVDAQISYELNAAQMLKKQGNEFHSQGRYKDALEKYMRAKNNLKGVPAAKGGTLLLACSLNLMSCYLKTGQYDECINEGTEVLVSDARNVKALYRRGQAYKNLGQLQKAVSDLSKALELSPDDETIADVLRDTRDKLSEQGDEDAPGGIKIEEIIDEVPTPLPEKDQASSSKSSEQKEDSNQVRSTAGFPNNDRESIRSFQNFLSQTDPETMASLGGGKFENLSPDMVKTASNMISKMPPEEFQKMLEMATSLQGGNGNPLLNRNNNESGMPNLSPEMIKSATEMMSKMPPEEMQKMFEMASSLNGGSQSNRNNSNNNLEGQESHRSSRDSGISGDSGPSRGFLNSTSAPQPNFPSSSADIRNQLKNPAMREMMSSMMKNMSPDMMANMSEQFGVKLSREDAERAQQAMSSLSPENLDRLMKWADRIQRVGENAVKTKNWLLGRQGLVLAICMLVFAMFLHWLGYIGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CES3</accession>
    <name>A0AAD5CES3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ATP-dependent RNA helicase</fullName>
            <ecNumber evidence="8">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_013890</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">RNA helicase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="domain">
        <text evidence="8">The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DEAD box helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="8"/>
    <dbReference type="EMBL" id="JAMZMK010008385">
        <property type="protein sequence ID" value="KAI7740642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CES3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd17946">
        <property type="entry name" value="DEADc_DDX24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24031">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 7" id="KW-0347">Helicase</keyword>
    <keyword evidence="2 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="13">
        <location>
            <begin position="163"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="11">
        <location>
            <begin position="195"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="12">
        <location>
            <begin position="341"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="73"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="631"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="543"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="6">
        <location>
            <begin position="163"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="79"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="114"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="645"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="657"/>
            <end position="683"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU365068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7740642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="683" mass="77133" checksum="0F7E5A71F50941CB" modified="2024-05-29" version="1">MSSVRPKKKQRQRNPSKPDAGQLSRLDSLRWTSSLPDAGDDDPFSSFAGLNDFEGGVLSLEEIDASEYGLELLEEIPKGGKKQKKQTKRKREESYGDEVVKEEDADGETVKEVKKSKKKKKKKTKKAKKPEESKDDEEEPVNVANTNEKTDVEEDEVDDSEYHAWYELRLHPLLMKSICRLKFKEPTPIQKACIPAGAHQGKDVIGAAETGSGKTLAFGIPILQRLLDEREKFERQAAERGEADEKIVSKGFLRALIITPTRELALQVTDHLKQVAVGTDVKVVPIVGGMSTEKQERLLKGRPEIVVGTPGRLWELMSGGEIHLVELHSLSFFVLDEADRMIDNGHFQELQSIIDMLPMVREGHAEDTENSLRHLSSLMKILNVNVWTLHAQMQQRARLKAIDRFRGSDHGILVATDVAARGLDIPGVRTVVHYQLPHSAEVYVHRSGRTARASADGCSIALISPKESSKFASLCRSFSKESFKQFPIEASYMPEVLKRLSLARQIDRVLRKESQDKADQSWFQRNAESIELMVDENDSEDERANKIRKKKASSAQLKKLQQELNSLLSRPLQPKTFSSRYLAGAGISPLLQQQFEELAKQKLADFGNRGDTNKKRKMVVIGQDCVEPLQALRSSGQEISMDTKGAAEKRRNLDNLRRKRKEDKRRLHDQKRKEKKRLKNGGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CGX6</accession>
    <name>A0AAD5CGX6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA helicase</fullName>
            <ecNumber evidence="4">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">M8C21_005294</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="25">AA19_3_7</strain>
            <tissue evidence="25">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DNA2/NAM7 helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="25">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="4"/>
    <dbReference type="EMBL" id="JAMZMK010008466">
        <property type="protein sequence ID" value="KAI7740314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CGX6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22318">
        <property type="entry name" value="DNA2_N-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.320.10:FF:000001">
        <property type="entry name" value="DNA replication helicase Dna2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051827">
        <property type="entry name" value="Cas4_exonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014808">
        <property type="entry name" value="DNA_replication_fac_Dna2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011604">
        <property type="entry name" value="PDDEXK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047187">
        <property type="entry name" value="SF1_C_Upf1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36531">
        <property type="entry name" value="CRISPR-ASSOCIATED EXONUCLEASE CAS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36531:SF6">
        <property type="entry name" value="DNA REPLICATION ATP-DEPENDENT HELICASE_NUCLEASE DNA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08696">
        <property type="entry name" value="Dna2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0227">DNA damage</keyword>
    <keyword evidence="17" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0235">DNA replication</keyword>
    <keyword evidence="16" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="12" id="KW-0347">Helicase</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-0408">Iron</keyword>
    <keyword evidence="15" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA replication factor Dna2 N-terminal" evidence="22">
        <location>
            <begin position="175"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase helicase" evidence="23">
        <location>
            <begin position="718"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase-like C-terminal" evidence="24">
        <location>
            <begin position="794"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="45"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="65"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00047995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF08696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF13086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF13087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="KAI7740314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="1042" mass="116142" checksum="C60EE528693A6842" modified="2024-05-29" version="1">MSMLEMQLSSCSTVKLLEGITLEGRNLDQEMISCGSIEKATDKSNASFRSPFMTPPSRHDKTSDSIDTNGESSQLGSRQHKKALLELLDQVEDVICVEDQVSTDTGTASTSYEIKQNNKIGINSDAAEGSSVGSTEKAKTKPANVYFLVFEVSEKQFVGSNGSQRTLKVLRLLDEECGEERSVYLSDDWFYSVIAPGDTVHVIGDFSEEGICEVNRDKNFLIVHPDILVSGTRVAASFSCPRRTVLDERLKNNEQSSAALAGLIRESPTKEFLEEYARVLLQKNYESLFACGVYEGDIHRTMLEAIPRLLKWILLFRDSKSPSSCVDFGSNDGVKKVKVSEVIDIEEMAWAPKFGLKGVIDASMHVKVASKANQTTDYIMPLEFKTGKATNGQAAIEHSAQVMLYTLLMSERYKKNIDYGLLYYLHTDQTQGIAVRRSDLVGLIMRRNELASDLLKASTMQQLPPMLQVPNTCKGCRHLKACTVYHKAHGGSIEGSGLGDVYSSLVNHLTTAHCNFLKKWERLIDLEANELEVVRKEMWGSRSTNGSHSSTCLSSIVLDTSDQVPRNNFIQGNRFVYRFMRQDLNPSSSGKDLECSLKTGDYVILSTDPGRLVVGRGVIMDMTRINVTVSFSKRLRLPGSSPSKIIHDLCQEVWRIDKDEFTASFAMMRFNLIQMFLQDDRSSHLRSMIVDLNAPRYDGGCIFSQDPAVSYIWSEKTLNDDQRKAILKILTAKDYTLILGMPGTGKTSTMVHAVKALLMRGASILLTSYTNSAVDNLLIKLKSQSTEARENGMGVSLFCRLSEAHPQAISALQSQYRMSAPIMELSNALIYGNRLRCGSPDVANAKLVYTNSTSTSLWLKKVLDPLRQVIFINTDLLPGLEVKDCKTVNNPLEAYIVTEITDKLTCQGIRGEDIGVITPYNSQANLIKQYVSNSVEIHTIDKYQGRDKDCILVSFVRSSNNPKNCKSSLLGDWHRINVALTRAKKKLIMVGSQRTLSFVPILKLLIEKVEEGCSILNVNREDIDINNQHKGELKRCSQLRSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CKG1</accession>
    <name>A0AAD5CKG1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Amino acid transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_028352</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008056">
        <property type="protein sequence ID" value="KAI7742189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CKG1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48017">
        <property type="entry name" value="OS05G0424000 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="34"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="64"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="187"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="276"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="314"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="378"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="404"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="436"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="8">
        <location>
            <begin position="30"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7742189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="466" mass="51250" checksum="DA8CA23CEA8CABFA" modified="2024-05-29" version="1">MANRTTNDHHSPLLQYSNSSDEESLHLTHRTGTVWTAIAHIITGVMGAGVLSLGWSLAQIGWLFGPPAIVVFAAVTVVSTTILSDCYLSPHSEHGPDRNASFLQAVSYYLGEKSQWICAVLLNESLYGNDIAYTIATATSMRAILKSNCYHDQGHDAGCAYPDNVYMLLFGVVQILMSQIPNFHNMAWLSAVAAIMSFAYSTIGLGLGLANVIVNGEIKGSIGGVPAASTVDKVWLVFQGLGDIAFAYPYSIILLEIQDTLKPPSETKTMKKASRSAILITTLFYLCCGSFGYAAFGNNTPGNLLTGFGFYEPYWLVDLANACVILHLVGGYQVFSQPVFAIVETWFSDRYPDSHFVNGFYQLKLPLVTTFQLNPFRLCFRTLYVVSTTGIAMLFPYFNQVLGVLGALNFWPLVVYFPVEMYFVQRKILPWSRKWIALKTFSFGCFLVSAVGFIGSLEGLIMAKLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CKU9</accession>
    <name>A0AAD5CKU9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Replication factor A C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_003552</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007738">
        <property type="protein sequence ID" value="KAI7743369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CKU9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013955">
        <property type="entry name" value="Rep_factor-A_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08646">
        <property type="entry name" value="Rep_fac-A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Replication factor A C-terminal" evidence="1">
        <location>
            <begin position="8"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7743369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="158" mass="17541" checksum="E1E4A2696FA3C69A" modified="2024-05-29" version="1">MEANKPTTTICTVVSIDHTNLYYTVCSICEKTLPDPTTTATSSTRCNHCTGSTSKRLFRVLMSVATDTNVFVVVLFDRAARVLFGCSAQDLFDFTNIHPFCGETASKILEGEMLRVTLSNPRNGNAQHQRVVSVVPLRSGFRSVMDSLTELYRVRSDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CLF9</accession>
    <name>A0AAD5CLF9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Hexosyltransferase</fullName>
            <ecNumber evidence="13">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_014980</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Golgi apparatus membrane</location>
            <topology evidence="2 13">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the glycosyltransferase 31 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="13"/>
    <dbReference type="EMBL" id="JAMZMK010007699">
        <property type="protein sequence ID" value="KAI7743714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CLF9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008378">
        <property type="term" value="F:galactosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002659">
        <property type="entry name" value="Glyco_trans_31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214:SF290">
        <property type="entry name" value="BETA-1,3-GALACTOSYLTRANSFERASE 13-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214">
        <property type="entry name" value="BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01762">
        <property type="entry name" value="Galactosyl_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10 13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12 13" id="KW-0464">Manganese</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7743714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="236" mass="26877" checksum="446CB00C41DB1CE7" modified="2024-05-29" version="1" fragment="single">IVVFKQDITGVSNKCEPNKPLSVSVSWDHSNGGGGGDQNRYKVMGFVGIQTGFASVGRRRSLRKTWFPSDHQGLQRYMRLGCQDDPGELEESTGLAFRFVIGKTHDKSKMAALKKEVEEYDDFLLLDIEEEYSKLPYKTLAFFKAAYALFDSDFYERAARTLQTLNFRMFRNEDVTIGAWMLAMNVKHEINRQLCQPECTPTSIAVWDIPKCSGLCNPEKKLLELHQMKRCANSPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CLZ0</accession>
    <name>A0AAD5CLZ0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Thionin-like protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_020301</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007850">
        <property type="protein sequence ID" value="KAI7742971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CLZ0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038975">
        <property type="entry name" value="THNL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36312:SF1">
        <property type="entry name" value="OS01G0594500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36312">
        <property type="entry name" value="THIONIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042253071" description="Thionin-like protein 2" evidence="1">
        <location>
            <begin position="20"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7742971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="119" mass="12800" checksum="266382A0ECDFA20E" modified="2024-05-29" version="1" fragment="single">GLMKFVVVMGIVMVSGCRGDVNSFRDCYAKCFIFCMIEPNQSLCSCTTQCLKECIFPSLPGTMATDHPHTQNLGYCKLGCATSLCSNISKAHNPDGKNVESCVGSCTNKCTMNYSSSSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CR05</accession>
    <name>A0AAD5CR05_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DRBM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_014203</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006927">
        <property type="protein sequence ID" value="KAI7746641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CR05"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46031">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46031:SF26">
        <property type="entry name" value="DOUBLE-STRANDED RNA-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="117"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="28"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="126"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7746641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="232" mass="25285" checksum="E155D27171E4C1AF" modified="2024-05-29" version="1">MVELAGITFTGEPAKNKKQAEKNAAMSAWSSLKQLAKQDASSSSEPENNDEQEQIRIARALLNYKLKQKTGNKTASYNDLPVAFQRKFTTLAPRPQSPQRPPLTASKILPLIYQARPVSPGPRDPSPSATTVAAAPSSARQNRPISPHFSPYVPVRHYRAPHHGLAPPVTMRTAVPVFSAPPMSPAPRPVKIAPPICVRQAVPVFSAPPVKKEEVPKLNESDTIKCLQNLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CSA8</accession>
    <name>A0AAD5CSA8_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_020653</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006873">
        <property type="protein sequence ID" value="KAI7746924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CSA8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004709">
        <property type="term" value="F:MAP kinase kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050538">
        <property type="entry name" value="MAP_kinase_kinase_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48016">
        <property type="entry name" value="MAP KINASE KINASE KINASE SSK2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48016:SF5">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7746924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="61" mass="7113" checksum="A8575761842387C6" modified="2024-05-29" version="1">MQPAALFKVMKETPPIPETMSSDGKDFLRSCFVRNPAERPTARMLLEHKFLMVQNPREHTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CSC8</accession>
    <name>A0AAD5CSC8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Profilin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_011053</name>
        <name evidence="9" type="ORF">M8C21_011057</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Occurs in many kinds of cells as a complex with monomeric actin in a 1:1 ratio.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the profilin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006899">
        <property type="protein sequence ID" value="KAI7746752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAMZMK010006899">
        <property type="protein sequence ID" value="KAI7746756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CSC8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003785">
        <property type="term" value="F:actin monomer binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00148">
        <property type="entry name" value="PROF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.30">
        <property type="entry name" value="Dynein light chain 2a, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048278">
        <property type="entry name" value="PFN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005455">
        <property type="entry name" value="PFN_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036140">
        <property type="entry name" value="PFN_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027310">
        <property type="entry name" value="Profilin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11604">
        <property type="entry name" value="PROFILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11604:SF49">
        <property type="entry name" value="PROFILIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00235">
        <property type="entry name" value="Profilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00392">
        <property type="entry name" value="PROFILIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01640">
        <property type="entry name" value="PROFILINPLNT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00392">
        <property type="entry name" value="PROF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55770">
        <property type="entry name" value="Profilin (actin-binding protein)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00414">
        <property type="entry name" value="PROFILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7746752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7746756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="87" mass="9336" checksum="C21DDABB4C693899" modified="2024-05-29" version="1" fragment="single">MSWQAYVDDHLMCEIEGNHLSAAAIIGHDGTVWAQSATFPQVKPEEITGIMNDFNEPGSLAPTGLYLGGTKYMVIQGEAGAVIRGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CSU9</accession>
    <name>A0AAD5CSU9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_022661</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the syntaxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006798">
        <property type="protein sequence ID" value="KAI7747287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CSU9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048278">
        <property type="term" value="P:vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15844">
        <property type="entry name" value="SNARE_syntaxin5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010989">
        <property type="entry name" value="SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045242">
        <property type="entry name" value="Syntaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021538">
        <property type="entry name" value="Syntaxin-5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006012">
        <property type="entry name" value="Syntaxin/epimorphin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957:SF228">
        <property type="entry name" value="SYNTAXIN-31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05739">
        <property type="entry name" value="SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11416">
        <property type="entry name" value="Syntaxin-5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47661">
        <property type="entry name" value="t-snare proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00914">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0653">Protein transport</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="4">
        <location>
            <begin position="255"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="38"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="170"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="174"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="208"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7747287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="333" mass="36511" checksum="6B587A25BF2B9DF7" modified="2024-05-29" version="1" fragment="single">TEALSMGSMSSGGTHTFRDRTNEFRSLFDTLKKIATTTPVTNTGGSHHQQTDPSTSFKPPAAAASSYQSNFNKKASRIGLVSKKSSIFDDPLKEIQELTALIKNDITALNVAVSDLQTLQNMEIADGNYSEDRVVHSTAVCDDLKNKLMVATKKFQDVLTARTENIKAHENRRQIFSTNITRENPLRQQQTKTMMEPPPWSSPSKSSGNLQQSESSGNGVQAGNQLRRRLAVDGTPSSSQHMGASMLQQVIPQQEHLTQNRAVALQSVESTITELSGIFTNLATMVAHQGELAIRIDDNMEETLSHVEGARGALLKHLNQISSNSMNLSKIYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CSY9</accession>
    <name>A0AAD5CSY9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">2Fe-2S ferredoxin-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_002970</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the adrenodoxin/putidaredoxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006797">
        <property type="protein sequence ID" value="KAI7747299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CSY9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140647">
        <property type="term" value="P:P450-containing electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001055">
        <property type="entry name" value="Adrenodoxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23426:SF65">
        <property type="entry name" value="FERREDOXIN-2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23426">
        <property type="entry name" value="FERREDOXIN/ADRENODOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00111">
        <property type="entry name" value="Fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="2Fe-2S ferredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="7">
        <location>
            <begin position="104"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7747299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="171" mass="18857" checksum="2EC3F28349CDA843" modified="2024-05-29" version="1">MAFTVNLPPLPLRSLETATGIDKLPKPIMSFTRKKPILSFAITNSAEQPQTTTPEIELEFIGPKACSDGKYPVDRATAVSGEKLLRNIMKDNKLELYGTYGKLMNCGGGGSCGTCIVEVVDGKELLNERTNTELKYLKKKPESWRLACQTIVGNKENSGKVVIQRLPQQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CUQ6</accession>
    <name>A0AAD5CUQ6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">M8C21_027891</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="24">AA19_3_7</strain>
            <tissue evidence="24">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010006619">
        <property type="protein sequence ID" value="KAI7747967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000400">
        <property type="entry name" value="Nuclear pore complex protein NUP107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000015">
        <property type="entry name" value="Somatic embryogenesis receptor kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000016">
        <property type="entry name" value="Somatic embryogenesis receptor-like kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051824">
        <property type="entry name" value="LRR_Rcpt-Like_S/T_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48006">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN DDB_G0281931-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48006:SF90">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="14 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-0675">Receptor</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8 22" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="22">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041908535" description="non-specific serine/threonine protein kinase" evidence="22">
        <location>
            <begin position="20"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="212"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="276"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="556"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="569"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="KAI7747967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="596" mass="66284" checksum="2298A29B2B7B2F6D" modified="2024-05-29" version="1">MKLQYLFCCLVIFFTYAYALLSPAGVNYEVQALMNIKNGLDDPHSVLNWDADAVDPCSWTMITCSPDKLVIGLLLQNNRILGPLPVELGRLPKLQTLDLSNNMLTGAIPPSLANIKTLQYLRLNNNSLTGPIPQPLANMTQLTFLDLSYNNLSGPVPKFQAKTFNIMGNPIICATGKEQDCNGTTPIPMSLXXXXGSQQKSQPSVTPKTNKMALAFGTSLGCFCLLFFGFGFAFWWRRRFNQQVFFDMNEQYREEVCMGNLRRFQFRELQVATSNFTSKNILGKGGFGIVYKGILSDGTVVAVKRLKDGNAVGGEIQFQTEVEMISLAVHRNLLRLYGFCMTSSERLLVYPYMSNGSVASRLKAKPALDWGTRKRIALGAARGLLYLHEQCDPKIIHRDVKAANILLDDYCEAVVGDFGLAKLLDHHDSHVTTAVRGTVGHIAPEYLSTGQSSEKTDVFGFGILLLELITGQRALEFGKSANQKGAMLDWVKKIHHENKLDMLVDKDLKTNYDRIELEEMVQVALLCTQYQPGQRPKMSEVARMLEGDGLAEKWEASQRAEANGGRANEFSHSERYSDLTDDSSLLAQAMELSGPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CVY0</accession>
    <name>A0AAD5CVY0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Bromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_015768</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006636">
        <property type="protein sequence ID" value="KAI7747919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CVY0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd04369">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051831">
        <property type="entry name" value="Bromodomain_contain_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22881">
        <property type="entry name" value="BROMODOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22881:SF31">
        <property type="entry name" value="CHROMATIN REMODELER BROMODOMAIN FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bromo" evidence="4">
        <location>
            <begin position="182"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="281"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="460"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="37"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="103"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="115"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="282"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="473"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="495"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="528"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="566"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7747919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="593" mass="65444" checksum="FC4240AE59389362" modified="2024-05-29" version="1" fragment="single">REKHKQKHKPCLSMGKQQPGGGGGATTTKKKKKGRPSLLDLQKRSLQQQQQNINNNNNNNNNNKQQQQQLKKRSSRRNPNTSSSDDDDERKQKKVKLVVRLPQSDKKQENPHLIRSSSVNSASCGSDSNANVDNQIPISGSDLQGEKVSKAMDTLHGSPLESGPTTPLPDRKLLVFILDRLQKKDTHAVFSEPVDPDELPDYHEIIKQPMDFGTVRSKLDGGLYSNLEELESDVYLICSNAMQYNSSDTVYFRQARAIQELAKRDFENLRIEGEDGELQPKVVRRGRPPGKLVKKTPGRPPLDRVGPESTSGATLATTIEDNTNESTPYNLRKAPPMLYKFQADGLLGSHRSRNGEHYSELMADWNEEFPERIRRADMKYGNKNLIIDETRRDTYKQFHPSAYAYESSLLSNFGGERKQLMAVGLHAEHGYARSLARFAANLGPVVWKIASKKIEKALPPGTKFAPGVVGEDPSPPSSFNPPGNPRPIPGLVPDSISNKPQTPSCSGQNPSTVNHPKVEMIEPGSGIRTDSSSNHVRNGFNSVFGSSTPAEHNRVGEAGCGPAKPTSWQQVPSSNPVAVQPDVNPNESNGDDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CWL2</accession>
    <name>A0AAD5CWL2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TPX2 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_029968</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TPX2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006480">
        <property type="protein sequence ID" value="KAI7748710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027329">
        <property type="entry name" value="TPX2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044216">
        <property type="entry name" value="WDL7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47067">
        <property type="entry name" value="TPX2 (TARGETING PROTEIN FOR XKLP2) PROTEIN FAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47067:SF23">
        <property type="entry name" value="TPX2 (TARGETING PROTEIN FOR XKLP2) PROTEIN FAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06886">
        <property type="entry name" value="TPX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TPX2 C-terminal" evidence="7">
        <location>
            <begin position="391"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="77"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="130"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="348"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="458"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="149"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="372"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="471"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="518"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="541"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="604"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="626"/>
            <end position="654"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF06886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7748710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="676" mass="74512" checksum="38AA695F272DF65A" modified="2024-05-29" version="1">MAGEIEEPFHLNFQADLLRSGSISFGRFESESLSWERRSVFSHNRYLEEVEKYSKPGSVTEKKAYFEAEFRRKALLKQRASEGQDGGESPTDGNGDLRDFEEYGNCDESPCSSDLSKCNTETEVQYANADVASRNSGYDEDNGISQHSESQKYELHRVTSQDELGETGQSENENVAGNTEQSIGLTIVDHVVPVDMSSEAHGDSSLACQIPEMDDDSISSTPIAITTVVDHLVLADVSSEAHDDSSLACQIPEKDDDSIRSAPIAMTTVSPKVKPAAEKKFTRSTLKTQINVDSSQKKVLNGASKGLMKPRTSESKGLIVTKTEKKSPRPASPSIGSRVKTLKAEVLRSEKAVKAQRPLSEKSVPGRPTVNRPKQSVNPSKTNIVRSATGFSFKTDQRAENRKEARIFYTKIAEKIHAKEVEINQVEAKILEKQAAEMKQFRKSLNFKAKPLPSFYNESARASDQHKATPISKTPNQQRSSLQAMATRHEKNPLKSSIPLNTRPSSSTSSSRRDKNPFKSSVPLNNRPGSSTSATRHDNNPVKSSVPLNNRPSSSMSSTRQGKNPLKSSVPLNNRPSSSTSSTRRDKIPLKSSAPXXXXVPLNIRPSSSSSSINRIRVSESPVRNHVPEKKDKEKGKDTKLNKQSDPEPRGRDMMRKYVNVKCSHTWNGPKVGIAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CYL5</accession>
    <name>A0AAD5CYL5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Bromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_013053</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006093">
        <property type="protein sequence ID" value="KAI7750751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CYL5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000627">
        <property type="entry name" value="Bromodomain and WD repeat domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000440">
        <property type="entry name" value="Bromodomain and WD repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000403">
        <property type="entry name" value="PH-interacting protein isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052060">
        <property type="entry name" value="Bromo_WD_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057451">
        <property type="entry name" value="BRWD/PHIP_AD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16266:SF38">
        <property type="entry name" value="TRANSCRIPTION FACTOR WD40-LIKE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16266">
        <property type="entry name" value="WD REPEAT DOMAIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25313">
        <property type="entry name" value="BRWD_AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="102"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="144"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="186"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="252"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="442"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="7">
        <location>
            <begin position="1211"/>
            <end position="1254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="653"/>
            <end position="965"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="722"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="758"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="778"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="839"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="858"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="891"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="901"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="921"/>
            <end position="934"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="935"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="952"/>
            <end position="965"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7750751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="1271" mass="143758" checksum="1AC82AF08D64E0B7" modified="2024-05-29" version="1">MAPSRGIFGKSPPTAADVPTLLGIGPFSLLGCTKNDTGPHVKPLPAYLRWPHMQADQVRGIGLREIGGGFPKHHRAPSIRFASYAIAKPSTMVQKMQNIKKLRGHRDAVYCAIFDRTGRYVITGSDDRLVKIWSMETAFCLASCRGHEGDITDLAVNLNNTLVASASNDYNIRVWRLPDGMPISVLRGHIGAVTAIAFSPRHSSVFQLLSSSDDGTCRIWDARHSQGCARIYVPRQTNNVAGSNNGPSSSTSSQQNNQILCCAYNANGTIFVTGSSDTFARVWSACKPNIDDSQQPVHEIDVLSGHENDVNYVQFSGCAVTSRSLTSDTSTDEKVPKLKTLWYSHDNIVTCSRDGSAIIWILKSRKSHGKVGRWTRAYHLKVPPPPMPPQPRRGGPRQRFLPTPRGVNMIVWSLDNRFVLAAIMDCRICVWNASDGRLVHSLTGHSESTYVLDVHPFNPRIAMSSGYDGKTIIWDIWEGIPIRIYEIGRFKLVDGKFSPDGTSIVLSDDVGQIYLLNTGQGESQKAAKYDQFFLGDYRPLARDAQGYAVDEETQLPPYKRNIQDALCDSSMLPYPEPYQSDYQRRRLGALKLEWRPPSLKFAIGTDIGLGQEYQMLPLADLDMVPEPQLELLDATLWEPENDIIIDSTDSEYNVTDDSEDEQASLCNSSVDAAESSGGNNIDDASRRRSKRKKPKDSEWTSSSEQRVKRRNTNEHEGGTSSRNKRTKKSKKNRNPKVTSSRAQRASARNGKKISSPEFGDDEEEEEEDYVSEHYTSESEPDIEEEEEEDEEEVKEEEEKEIEEKEEEEKEEAEKPKVSIPPESKPKLVLKFSIPKKSPVPQNTQNTLNRCNEPSTSSRPEKEEEIAKPSENHNTTKPTKIRIKPSGGECSQVKKEDDIGSQKKKTIKFKFKTSNAKENDNSSEVDSMKFQSSSRETNDAPQDHKNSVHLIPSNSSMASGSGSKSNGQLSKTNWLLLSGQEWRCRYIPQLGDEVAYFIQGHQEYIKDKASSSERGPWALFKNKVIRDVEFCLVSRLDYVNLAGSGESGCKLKLKFIDPLSDVLGKTFELTLPELVDSPDFLVERTRYEISLERDWSVGDKCSVWWRDEGGGGSMWHGFIESICDKSVDFPGSQWERYLVKYESEYERHSPWEFDDKDSEWEQPGIDLDIRKKMLYFLSELEHSARGNKDKYGIMKLKQTTERLEFINRFPVPLTLEIIELRIKNFYYRSVNAMKHDIMVMLSNAETYYTKHTEMKVKVKRLSERFERELSEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D156</accession>
    <name>A0AAD5D156_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_027804</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005955">
        <property type="protein sequence ID" value="KAI7751192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D156"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33474:SF27">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33474">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041993697" evidence="2">
        <location>
            <begin position="25"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7751192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="94" mass="10556" checksum="89C7B8AC68263D77" modified="2024-05-29" version="1">MAANSFYLIVTLLAFSHLLFMADAISTSGKVHRHMLHGKVEILASGHNKDQMINVEESSFLEDEFMSERMDLEKNDYPGPGANRNHTPKPPRGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D291</accession>
    <name>A0AAD5D291_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PROP1-like PPR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_016917</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005923">
        <property type="protein sequence ID" value="KAI7751369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D291"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051222">
        <property type="entry name" value="PPR/CCM1_RNA-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033443">
        <property type="entry name" value="PROP1-like_PPR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47942:SF50">
        <property type="entry name" value="OS03G0284900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47942">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT (TPR)-LIKE SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17177">
        <property type="entry name" value="PPR_long"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PROP1-like PPR" evidence="4">
        <location>
            <begin position="238"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="368"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="403"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="526"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="561"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="596"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="25"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="40"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF17177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7751369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="728" mass="81273" checksum="0E5CDEEAEBFE0E66" modified="2024-05-29" version="1">MRITTTTTTTTITASSISTPPPLPKHTRTTTKPKHSSKQPKPPPPPLPSPSSSTKSHRPHLFNTFQWNTHTNINYYAELASKLAQDARFDDFFMIVETVTSDSASSVDFSALLNAEIISNGILSVIESDEGVQRLNKVLTGFQKLGIGSVVKVLLSDRSVVNAIGRECRSVLNRGGVDDVVDFMETLSGFGLTIEDVMEPVEVIRNCVNKRNLHAVIRYAGLCARADEVFCSGILEFGKRGDMASALTVFEESKRKLGCVNMYAYRTIIDVCGLCHDHLQSRTIYEELNAQNINPNLYVFNSLMNVNARDLNYTMNIYKHMQNLGIAPDIASYNILLKSCSLAARRDLAQEIYEEVEHLESIGSLKLDVFTYSTMIKVFTDERMWQMALGIKDKMLEAGVTPNIVTWSSLISACAKAGLVDQAFVLFEEMLLAGCMPNSQCCNVLLHACVKALQYDRAFRLFQNWKNNSFQLTNGNLTLGGSQTAINVPFKPTVSTYNVLMKACGTDHFLAKALMEEMETVGLSPNHISWSTLIGVYGGSGDVKRAVQVLKTMRESGVPPDVIAYTAAIKVCVKQRELNLAFSLFAEMKRYQIQPNMVTYNTLLRARTRYGSLIEVQQCLSIYQDMRKSGFKPNDYYLKELIEEWCEGIIQDNHRSQGQLKNTSRRDLGGPHSLLLEKVASHLQKGTQSIAVDLRGLTKVEARIVVLAVLRMIKENYHPGNGEFFENL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D2U3</accession>
    <name>A0AAD5D2U3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_009213</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005766">
        <property type="protein sequence ID" value="KAI7752032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D2U3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7752032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="148" mass="16833" checksum="9E60176E6E60C748" modified="2024-05-29" version="1" fragment="single">PHSSSERGERKSIVKVKREREKVTWSITRFAPPPPPPSELPPQPIIKILSATQVFDEMILKPKVIASEETSIHQVRLPSPYTTKAKRTNNIYMAIQFPRSKGLKPPHEPTHAIAAAIQGNHTPQREWRIKSDTAGKMVILTFHLMKYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D462</accession>
    <name>A0AAD5D462_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Homogentisate phytyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_029085</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UbiA prenyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005009">
        <property type="protein sequence ID" value="KAI7753953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D462"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031969">
        <property type="term" value="C:chloroplast membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016765">
        <property type="term" value="F:transferase activity, transferring alkyl or aryl (other than methyl) groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.140">
        <property type="entry name" value="UbiA prenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1780">
        <property type="entry name" value="UbiA prenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044878">
        <property type="entry name" value="UbiA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43009:SF9">
        <property type="entry name" value="HOMOGENTISATE PHYTYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43009">
        <property type="entry name" value="HOMOGENTISATE SOLANESYLTRANSFERASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="42"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="102"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="150"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="206"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7753953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="229" mass="25426" checksum="60612D3E4AC58F38" modified="2024-05-29" version="1" fragment="single">VNKPSLPLAAGELSMTTATIVSTLSAIMGFSIAWIVGSPSLFWGFVGWFAAATAYSANVYPLLRWKRFPFATAFYMLISKALVVPVAYYMHMQNSINGGASLVSRPLLFAVGMLSAFSLSTIFFKDIPDIEGDRMHGIKSLAIRLGEKRVYWTCIWILEMAYVAAALVGATSSITWSKYATIISHLAMGMLLWIRAKSVDVNNAKAVQSMYMFLWQLFYVEYGLILLVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D4H9</accession>
    <name>A0AAD5D4H9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Isopenicillin N synthase-like Fe(2+) 2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_011687</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the iron/ascorbate-dependent oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005274">
        <property type="protein sequence ID" value="KAI7753768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D4H9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10209:SF429">
        <property type="entry name" value="1-AMINOCYCLOPROPANE-1-CARBOXYLATE OXIDASE HOMOLOG 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10209">
        <property type="entry name" value="OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Isopenicillin N synthase-like Fe(2+) 2OG dioxygenase" evidence="5">
        <location>
            <begin position="121"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7753768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="197" mass="21745" checksum="6560C1902B6CCFAB" modified="2024-05-29" version="1" fragment="single">GVKGLVDAAAGGVVDIPNIFIRPPDEVAEDLELSRSSLQVPVIDLNGVGEKGTNWRDTLVIDMAYTYDLHLHDLPSVCRESTVDYLKHVTKFVDTLFELLSEALGLDANYLKCMELDKGKTLVGHYYPACPKPDRTLGISKHTDASFITLLVQDDVGGLQVLYQNQWVSVDPISSALVVPTLSQKKTPYKRTKCLKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D5W5</accession>
    <name>A0AAD5D5W5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_015118</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005722">
        <property type="protein sequence ID" value="KAI7752210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D5W5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47303">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47303:SF1">
        <property type="entry name" value="NF-KAPPA-B INHIBITOR BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7752210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="248" mass="27560" checksum="ABBA331BBDAB99A6" modified="2024-05-29" version="1" fragment="single">MKQPSTGSGLSIPVIPSHHQQQQNHTIVPISPTTVPPPVPQLPNLPRTDLLEGSRENYIKVGVPLYEAAMKGNWKAAKPILDKQPDLIRFAITDNCETLLHIAATAERTKSVEEFLVNLITLMEIEDLELQNRNNNTALSLAAATGNVEAARMMVEKNQNLMEMVDGNGMMPLNLAALFAEVDTMTYLYNASKKMSGDCWSKENQGWVLQKCVEDLAIQIVKDHPQLMDDKEIATDVLFALARKPRAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D709</accession>
    <name>A0AAD5D709_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RNA polymerase Rpb4/RPC9 core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_014107</name>
        <name evidence="6" type="ORF">M8C21_014112</name>
    </gene>
    <organism evidence="5 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the eukaryotic RPB4 RNA polymerase subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010001017">
        <property type="protein sequence ID" value="KAI7755566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAMZMK010001017">
        <property type="protein sequence ID" value="KAI7755571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D709"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030880">
        <property type="term" value="C:RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.40:FF:000004">
        <property type="entry name" value="Dna-directed rna polymerase ii subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010997">
        <property type="entry name" value="HRDC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006590">
        <property type="entry name" value="RNA_pol_Rpb4/RPC9_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045222">
        <property type="entry name" value="Rpb4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005574">
        <property type="entry name" value="Rpb4/RPC9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038324">
        <property type="entry name" value="Rpb4/RPC9_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21297">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03874">
        <property type="entry name" value="RNA_pol_Rpb4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00657">
        <property type="entry name" value="RPOL4c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47819">
        <property type="entry name" value="HRDC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNA polymerase Rpb4/RPC9 core" evidence="4">
        <location>
            <begin position="16"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7755566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7755571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="138" mass="15980" checksum="E1491E247132F7BE" modified="2024-05-29" version="1">MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPMNQVSQVFEKSLQYVKRFSRYKNPDAVRQVRELLSRYQLAEFELCVLGNLCPETVEEAIAMVPSIKSRGRGHDDEAIEKMLNDMSLIKKFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D8H1</accession>
    <name>A0AAD5D8H1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">M8C21_021409</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">AA19_3_7</strain>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010005380">
        <property type="protein sequence ID" value="KAI7753575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D8H1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048544">
        <property type="term" value="P:recognition of pollen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00028">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01098">
        <property type="entry name" value="PAN_AP_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000060">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.90.10.10">
        <property type="entry name" value="Bulb-type lectin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001480">
        <property type="entry name" value="Bulb-type_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036426">
        <property type="entry name" value="Bulb-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000858">
        <property type="entry name" value="S_locus_glycoprot_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002">
        <property type="entry name" value="RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE SD1-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002:SF932">
        <property type="entry name" value="RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01453">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08276">
        <property type="entry name" value="PAN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00954">
        <property type="entry name" value="S_locus_glycop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00108">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51110">
        <property type="entry name" value="alpha-D-mannose-specific plant lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50927">
        <property type="entry name" value="BULB_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50948">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Bulb-type lectin" evidence="15">
        <location>
            <begin position="2"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="16">
        <location>
            <begin position="202"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="329"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="17">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAI7753575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="782" mass="88688" checksum="2DD4ED37E77C7BA7" modified="2024-05-29" version="1" fragment="single">SSDTIAVHQNFSDGQTIVSANTRFELGFFSPGNSKNRYLGIWFKNTSPQTFVWVANRETPLIDTLGTVKLDNQGNLTLVNGSGEIIWSQLLDTGNLVIRNGNKTNNEIVIWQSLDYPGDTYLPGMKMGKNFITGRETYPTSWRSADDPSPVSPGGKLENFQLNLPNPEWLQSNSLPVDYCDNYEVCGPYGSCNTATYPYCGCLKGFERKTSDDTSPDNDTSVCKRSIALNCGPGEGFKNCEIACKNNCSCTAYANPNITPGGVGCLLWFGDLIDVRVYPQNGQALYVRLAASELSVVLTLLGRILALYIRGKRKKRSNAEREGKATNDFSVENKLGEGGFGPVYKGVLEEGLEIAVKRLSKFSRQGLDEFENEVMCIAKLQHRSLVRLLGYCTHGDDMNICLTKVSTVFYLVHESRKLLLDWPQRFQIFQGIARGLLYLHQDSRLKVVHRDLKARNILLDHQMHPKISDFVLAKMFKEDESEANTKRVVGTLGYISPEYAVNGLFSVKSDIFSFGVLVLEIVSGKKNTGFIHEDHDDNLLAYAWRLHKEGKSLDLIDECLLHVGLLCVQQRVEDRPNTRTVVRMLDGEGFEKTKGEKHTYCYDINRGRCKCILTMDDEVLEITDDATTSDKERGFKFTLFVTIYKSRKLSARRSMKCLDEEFGLWKKEKKPFDWTDMGWNWLKAAETGNMAMQFVLSECMSNEIELDQEIQLSLKFWIDTGWVCNKECKDDNITEYDAEIVLKKLPPERQSMLSCWAKKLSRKYMCNLLKTEIRGMENMMKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D8Z9</accession>
    <name>A0AAD5D8Z9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cationic amino acid transporter C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_029115</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily. Cationic amino acid transporter (CAT) (TC 2.A.3.3) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005260">
        <property type="protein sequence ID" value="KAI7753815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D8Z9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029485">
        <property type="entry name" value="CAT_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43243:SF41">
        <property type="entry name" value="CATIONIC AMINO ACID TRANSPORTER 7, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43243">
        <property type="entry name" value="INNER MEMBRANE TRANSPORTER YGJI-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13906">
        <property type="entry name" value="AA_permease_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="44"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="68"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="109"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="152"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="196"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="248"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="272"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="305"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="334"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="364"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="396"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="domain" description="Cationic amino acid transporter C-terminal" evidence="7">
        <location>
            <begin position="365"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7753815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="445" mass="48542" checksum="B0044F268FB20F9C" modified="2024-05-29" version="1">MDYVFSNAAVARSFTTYLGTAIGVSAESQWRITITSLPKGFNQIDFIAVIVIVILTVIICYSTRESSVLNMILTALHILFIMFVIIMGFLRGDSKNFTEPSNPTQPGGFFPFGASGVFNGAALVYLSYIGYDAVSTLAEEVKNPVTDIPIGVTGSVILVTVLYCLMATSMSMLLPYDLINPEAPFSGAFVGKSNRWNWVSNVIGVGASFGILTSLLVAMLGQARYLCVIGRSNIVPVWFAKVHPKTSTPVNASAFLGIFTAAIALFTELDVLLNLVSIGTLFVFFMVANAVIYRRYVSVETPSPWPTLSFLICFSCTSVMFTLLWWLVPPGKPKGFVLGACLIVVCGLVVVFNYAVPQARKPEFWGVPLMPWIPCVSIFLNIFLLGNLDGPSYKRFGFFSALAVVVYLVYSVHASFDAEEEAIGNLESGIKESIELEDLTPKLQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D9F2</accession>
    <name>A0AAD5D9F2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_014857</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Co-chaperone that regulates diverse cellular pathways, such as programmed cell death and stress responses.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000235">
        <property type="protein sequence ID" value="KAI7756901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D9F2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000774">
        <property type="term" value="F:adenyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17054">
        <property type="entry name" value="Ubl_AtBAG1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000298">
        <property type="entry name" value="BAG family molecular chaperone regulator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.120">
        <property type="entry name" value="BAG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039773">
        <property type="entry name" value="BAG_chaperone_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036533">
        <property type="entry name" value="BAG_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003103">
        <property type="entry name" value="BAG_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12329:SF11">
        <property type="entry name" value="BAG FAMILY MOLECULAR CHAPERONE REGULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12329">
        <property type="entry name" value="BCL2-ASSOCIATED ATHANOGENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02179">
        <property type="entry name" value="BAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00264">
        <property type="entry name" value="BAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63491">
        <property type="entry name" value="BAG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51035">
        <property type="entry name" value="BAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="4">
        <location>
            <begin position="28"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="BAG" evidence="5">
        <location>
            <begin position="123"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="208"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="208"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="233"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00058673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7756901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="292" mass="33009" checksum="1E17EC18922F7010" modified="2024-05-29" version="1">MMPTEELEVRPGGMLVQKRDPNEEHIPPTIRVRVKYGSLYHEINISSQATFGELKKMLSGPTGLHHEDQKLMYKNKERSSKTFLDVVGVKDRSKMVLVEDPISQERRYVEMRKNAMMEKAAKSISEISLEVDRLAGQVSAHESVISKGGKVAEKALLILIELLMNQLLKLDEITAEGDVKLQRKMQVKRVQKYVETLDVLKIKNSIQVEEQPASRNQEHGCSNTDRPRPFPNSRRSVGNFSSVPVVPKQQQPSTSGEVVVTTKWETFDSLPTPLPAPPSNTINPKFSWDLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D9P2</accession>
    <name>A0AAD5D9P2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein YIP</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_014487</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Golgi apparatus membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the YIP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000360">
        <property type="protein sequence ID" value="KAI7756498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D9P2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006977">
        <property type="entry name" value="Yip1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039765">
        <property type="entry name" value="Yip5/YIPF1/YIPF2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12822">
        <property type="entry name" value="PROTEIN YIPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12822:SF2">
        <property type="entry name" value="PROTEIN YIPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04893">
        <property type="entry name" value="Yip1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="124"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="169"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="198"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="224"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="255"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="Yip1" evidence="8">
        <location>
            <begin position="112"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="24"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="64"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7756498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="281" mass="30911" checksum="4665D8E30632E440" modified="2024-05-29" version="1">MMSGNYTTIDNQNVSGSVPAVSDSPGQVSVKFNDTSLQTFPPSGAQGKISAAAGPPRDADDTFSKPASGSPESPSSGGWFKMFTVAAYRPYFDVDTSDVLERIKDSLLPFNGSFNEKTANNPDLYGPFWICTTLIFVAASIGTFVTYLAHKLQHKEWNYDINLVTWSAGVFYGYVLIVPLCLYVILKYFSAPSTLVQLFCLYGYSLFVFIPAMCLSVIPVELFRWVITGVAGIMSAMFVALNLRNHIVSAGERWFLIVVGIFLLQLALSFVLKLYLFNVSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DAP8</accession>
    <name>A0AAD5DAP8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 8">Cytokinin riboside 5'-monophosphate phosphoribohydrolase</fullName>
            <ecNumber evidence="2 8">3.2.2.n1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_016474</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 8">Cytokinin-activating enzyme working in the direct activation pathway. Phosphoribohydrolase that converts inactive cytokinin nucleotides to the biologically active free-base forms.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>9-ribosyl-trans-zeatin 5'-phosphate + H2O = trans-zeatin + D-ribose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:48564"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16522"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:87947"/>
            <dbReference type="EC" id="3.2.2.n1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 8">
            <text>N(6)-(dimethylallyl)adenosine 5'-phosphate + H2O = N(6)-dimethylallyladenine + D-ribose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:48560"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17660"/>
            <dbReference type="ChEBI" id="CHEBI:57526"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="EC" id="3.2.2.n1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the LOG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.n1" evidence="2 8"/>
    <dbReference type="EMBL" id="JAMZMK010000097">
        <property type="protein sequence ID" value="KAI7757583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DAP8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016799">
        <property type="term" value="F:hydrolase activity, hydrolyzing N-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009691">
        <property type="term" value="P:cytokinin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.450:FF:000013">
        <property type="entry name" value="Cytokinin riboside 5'-monophosphate phosphoribohydrolase LOG8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005269">
        <property type="entry name" value="LOG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031100">
        <property type="entry name" value="LOG_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00730">
        <property type="entry name" value="Rossman fold protein, TIGR00730 family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31223:SF11">
        <property type="entry name" value="CYTOKININ RIBOSIDE 5'-MONOPHOSPHATE PHOSPHORIBOHYDROLASE LOG8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31223">
        <property type="entry name" value="LOG FAMILY PROTEIN YJL055W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03641">
        <property type="entry name" value="Lysine_decarbox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102405">
        <property type="entry name" value="MCP/YpsA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0203">Cytokinin biosynthesis</keyword>
    <keyword evidence="4 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="193"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7757583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="213" mass="23314" checksum="00DFBD78279870AE" modified="2024-05-29" version="1" fragment="single">MEGGYSSSKFKKICVFCGSNPGHRDVFSVAATELGDELVNRKINLVYGGGSVGLMGMISQRVFDGGCHVLGVIPKALVPLEISGETVGEVRIVSDMHERKAEMAREADAFIALPGGYGTMEEVLEMITWSQLGIHKKPVGLLNVDGYYNSLLALFDNGVKEGFIKPSARDIVLSASNAIDLLTKMEEYKPEHDHVAPHESWQMEQLGNYPNKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DBZ2</accession>
    <name>A0AAD5DBZ2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Methyltransferase</fullName>
            <ecNumber evidence="7">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_014858</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Endomembrane system</location>
            <topology evidence="6">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the methyltransferase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="7"/>
    <dbReference type="EMBL" id="JAMZMK010000235">
        <property type="protein sequence ID" value="KAI7756902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DBZ2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004159">
        <property type="entry name" value="Put_SAM_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10108:SF1049">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10108">
        <property type="entry name" value="SAM-DEPENDENT METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03141">
        <property type="entry name" value="Methyltransf_29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7756902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="200" mass="22740" checksum="8C7806779994B46E" modified="2024-05-29" version="1">MVPPRVSSGSIDGVTPEIFQEDIILWKKRISYYTTVNSQLGQPGRYRNLLDMNAFLGGFAASLVDDPLWVMNIVPVEAKADTLGAIYERGLIGTYQSWCEAMSTYPRTYDLIHADSVFTLYKDRCEMEDIMLEMDRILRPEGSVIIRDDVDLLVKIKRIAEGLNWESQIVDHEDGPLEREKLLFAVKLYWTAPADSDQGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DC91</accession>
    <name>A0AAD5DC91_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_002353</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000220">
        <property type="protein sequence ID" value="KAI7756922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DC91"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7756922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="59" mass="6394" checksum="DC8F079808F0A96C" modified="2024-05-29" version="1">MLRGGSHIASDTRSKNSPCDCYQEMKAKSRTKASGIKTSSCEARDSIALANFKLPFNAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-02-05" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DCT3</accession>
    <name>A0AAD5DCT3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_017121</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000136">
        <property type="protein sequence ID" value="KAI7757337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051504">
        <property type="entry name" value="Plant_metabolite_acyltrans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31625">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02458">
        <property type="entry name" value="Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7757337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="187" mass="21218" checksum="8BF0DFB224DE74FB" modified="2024-05-29" version="1">MTNHHCLCDASINILFLKAWTSIARAGTNKSFLANGTMPFYGRVVKNPELDEKHLKFAEVEKFKEYQPFKLCGPTDKVRATFILSRRVINKLKTLVSTQLPTLPYVSSFTVACAYVWSCLAKTRENGLQLFRFGIDCRARMSPPIPAAYFGNCVVKGCNAMGKXXXXLVFAYRLWRWNLLFVSLNMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DDM4</accession>
    <name>A0AAD5DDM4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RHOMBOID-like protein</fullName>
            <ecNumber evidence="6">3.4.21.105</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_007497</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Serine protease involved in intramembrane proteolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>Cleaves type-1 transmembrane domains using a catalytic dyad composed of serine and histidine that are contributed by different transmembrane domains.</text>
            <dbReference type="EC" id="3.4.21.105"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the peptidase S54 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.105" evidence="6"/>
    <dbReference type="EMBL" id="JAMZMK010000250">
        <property type="protein sequence ID" value="KAI7756816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DDM4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1540.10">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002610">
        <property type="entry name" value="Peptidase_S54_rhomboid-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022764">
        <property type="entry name" value="Peptidase_S54_rhomboid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22936:SF107">
        <property type="entry name" value="RHOMBOID-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22936">
        <property type="entry name" value="RHOMBOID-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01694">
        <property type="entry name" value="Rhomboid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0720">Serine protease</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="22"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="54"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="85"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="124"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="149"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="197"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S54 rhomboid" evidence="7">
        <location>
            <begin position="14"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7756816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="290" mass="32605" checksum="91E5329AB49B07C1" modified="2024-05-29" version="1">MGALEYKKVIKESEEWRLLTCMWLHAGVFHVFANMLGLVLIGSRLEHEFGFVRIGALYILSGIGGSILSSLFVRATASVGASGAIFGLLGGMLSELITNWTIYANVGIRLFVHKHIFCFKYCQFVALSTLIVIILINIAIGILPHIDNYAHIGGFFTGFFLGFVILIRPQFKWINQRHVPHGYIAPTTRTKYKSYQCILLIISLIALLVGFTTGLILLTRGIDGNDYCSWCHYLTCIPTPLWRCESRCRLMQLDTQLNMTCLANQRSGSYTVEDPNDTVEMQKLCFELCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DE52</accession>
    <name>A0AAD5DE52_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_019281</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the MIP/aquaporin (TC 1.A.8) family. PIP (TC 1.A.8.11) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000053">
        <property type="protein sequence ID" value="KAI7757834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DE52"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015267">
        <property type="term" value="F:channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00333">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1080.10:FF:000001">
        <property type="entry name" value="Probable aquaporin PIP1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="entry name" value="Glycerol uptake facilitator protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034294">
        <property type="entry name" value="Aquaporin_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000425">
        <property type="entry name" value="MIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022357">
        <property type="entry name" value="MIP_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00861">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45687">
        <property type="entry name" value="AQUAPORIN OR AQUAGLYCEROPORIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00230">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00783">
        <property type="entry name" value="MINTRINSICP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81338">
        <property type="entry name" value="Aquaporin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00221">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="41"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="84"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="126"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="206"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="253"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7757834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="289" mass="30941" checksum="1324D74072E5667F" modified="2024-05-29" version="1">MTKDVEVAEHHGGGSEYSAKDYHDPPPAPFFDADELTKWSFYRALIAEFVATLLFLYVTVLTVIGYKSQTDPKLNADQCGGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLARKVSLIRALGYMIAQCLGAICGVGLVKAFQSSYYNRYGGGANELADGYNKGTGLGAEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIYNNEKAWDDQWIFWVGPFIGAFAAAVYHQFVLRAAAIKALGSFRSNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DG32</accession>
    <name>A0AAD5DG32_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">TFIIS N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_028485</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000185">
        <property type="protein sequence ID" value="KAI7757085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DG32"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00183">
        <property type="entry name" value="TFIIS_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.10">
        <property type="entry name" value="Conserved domain common to transcription factors TFIIS, elongin A, CRSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003617">
        <property type="entry name" value="TFIIS/CRSP70_N_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035441">
        <property type="entry name" value="TFIIS/LEDGF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017923">
        <property type="entry name" value="TFIIS_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46548">
        <property type="entry name" value="BAH AND TFIIS DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46548:SF1">
        <property type="entry name" value="BAH AND TFIIS DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08711">
        <property type="entry name" value="Med26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00509">
        <property type="entry name" value="TFS2N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47676">
        <property type="entry name" value="Conserved domain common to transcription factors TFIIS, elongin A, CRSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51319">
        <property type="entry name" value="TFIIS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TFIIS N-terminal" evidence="5">
        <location>
            <begin position="141"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="257"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="588"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="808"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="882"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1121"/>
            <end position="1141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="25"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="46"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="262"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="284"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="314"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="333"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="412"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="455"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="470"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="614"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="888"/>
            <end position="898"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7757085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="1167" mass="123205" checksum="6FAA1A49265D0E76" modified="2024-05-29" version="1">MHGAVQAGGRSPKPLNGPNGPAQLKASSDNVQNVSSHAKSKKREHGVHNSESTKRDRSSKVDDADSGQVRPEQILKTEIAKITDKGGLVDFVGVEKLVQLMRPDKTEKKLDLTCRTMLVDVISATDKFDCLGQFVHQKGLQVLDEWLQEIHKGKIGDGSPKENDKSIEEFLFSLLCALDKLPVNLHALQTCNVGKSVNHLRSHKNSEIQKKAMSLVSTWKRRVEAEMNIIETKSNTIRGGSLPNKQMISEVSHIGGARRVGGSSSEVGPKSSASQPSPLKAPQPKQSSGDAIAKSPTSPGSTKLSPSSAKEGRSTSQSPNNSQSCSSDHGKAGTSSVSKISSSDLHSKNSGNGFHGATIPGAQKEAGKTGYLSRSFASEKGSPTRHSTERVPDASLVDNGNSSSLIVRLPNTGRSPTQTGSLESAEDSSTISGKGSLSAPSKKQEQKISGKIDSLQGNNVPNPNTGNDLSRGKEGPILPDEEHGKKDASGSSSGVTPQPGKLYEPSYSSINALVESCAKFSEASVPPSIGDVGGINLLASVAAGEMSRSDLSPACSPDCSSHVPEGNSEGNNINVANGDVLVKQIGSVGGDTNNTGSVNMMEDATNMKPNSRSALEEDKCRDDKPIRNSVGPLDAAAASVKLETSQINEEAASLSSSDMHEDDKKLVHKRLTDQPVATTSCGDIDLKPKYEETDDKKAHENMDSGPSVSERAYGNMEKDNKAPVVAMQEVRTCGKVTDVTEANCAGDRTVKLDFDLNEVLPSDDGIQCDVEKAAIHAPNPLPSNNKSWSGLITVAAAAKGPFYPSESLSRGKPELGWKGSAATSAFRPAEPRKVDVPVPDSRTSKPARALFDFDLNVGVEDANQPNGSSGLDLDLNACEESPEAAHLSVSTSSRPSRSALPGGLNSSRDFDLNGPGMEEVGGESATFSKNGMQFISSVPNVRMNNMDMGNFSTWFPPNNSYSAITIPSQRMLTPVTSSTSLNPEMFRGPVLSSSPAVAFPSTAYQYSAFPFETNFSLPSISNTFSSVSNAYVDSSSSSGGPLCFPAIPSVGPNGVMSMPYRPYYMGLPGGSANVGPDGRKWGSQGLDLNAGPGDDKLATTLRQLPLGGGDEQLRMFHQMAAGGGSATKRKEPDGSWDGNRSVWNSPSEVLLSEVCGFPATSLVYDKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G1H2</accession>
    <name>A0AAD5G1H2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_032715</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the AB hydrolase superfamily. Epoxide hydrolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012107">
        <property type="protein sequence ID" value="KAI7724990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G1H2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000639">
        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43329">
        <property type="entry name" value="EPOXIDE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="3">
        <location>
            <begin position="39"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7724990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="330" mass="37327" checksum="F34526A8BFA67135" modified="2024-05-29" version="1">MHQFTTHTHTIAMDQITHKFVQVNGLKLHVAEIGLESSPVVIFLHGFPEIWYTWRYQMIAVAKAGFRAIAPDFRGYGLSEQPAEPEKATFADLINDTASILDSLNISKVFVIGKDFGAIVAYPFALQYPEKVAGIITIGLPFMPPGAFTTEFVLPEGFYPRRWQEPGRAEADFGRFDVKTVVRNVYILFSQSELQIANENQEIMDLVKPSTPLPSWFTEEDLEAYAALYEKSGFRTALQVPYRTMHMVGSEVQNPKVEVPTTMIMGEADYVLKIPGIGEYIRSGEVKKYVPNQETIYVPHGTHFLPEQFPEKVNELVLEFLNNHKHLVAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G2I6</accession>
    <name>A0AAD5G2I6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Hydroxyphenylpyruvate reductase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_016606</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011872">
        <property type="protein sequence ID" value="KAI7725727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G2I6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030267">
        <property type="term" value="F:glyoxylate reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016618">
        <property type="term" value="F:hydroxypyruvate reductase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12156">
        <property type="entry name" value="HPPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000213">
        <property type="entry name" value="Putative 2-hydroxyacid dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050223">
        <property type="entry name" value="D-isomer_2-hydroxyacid_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029752">
        <property type="entry name" value="D-isomer_DH_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10996:SF178">
        <property type="entry name" value="2-HYDROXYACID DEHYDROGENASE YGL185C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10996">
        <property type="entry name" value="2-HYDROXYACID DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00065">
        <property type="entry name" value="D_2_HYDROXYACID_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase catalytic" evidence="5">
        <location>
            <begin position="1"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding" evidence="6">
        <location>
            <begin position="51"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7725727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="255" mass="27918" checksum="DB1554CC8C5A054A" modified="2024-05-29" version="1" fragment="single">RDLIDSLPALEIISSFSVGLDKVDLEYCKEKGVRVTNTPDVLTDDVADLAIGLVLATLRRICEADRFVRGELWKKDEFKMTMKFSGKKVGIVGLGRIGTAIAKRAESFNCTISYHSRSEKPELIYKYFSSVIKLAANCDILVVACALTEETRHIINREVINALGPKGVLVNIGRGAHIDEPELVSALVEGRLAGAGLDVFEHEPHVPEELFGLDNVVMLPHVASSTVETEIAMADLVVANLEAHFLKKPLLTQVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G3C6</accession>
    <name>A0AAD5G3C6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Cardiolipin synthase (CMP-forming), mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_020197</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011749">
        <property type="protein sequence ID" value="KAI7726118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G3C6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043337">
        <property type="term" value="F:cardiolipin synthase (CMP-forming)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032049">
        <property type="term" value="P:cardiolipin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1760:FF:000020">
        <property type="entry name" value="cardiolipin synthase (CMP-forming), mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050324">
        <property type="entry name" value="CDP-alcohol_PTase-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043130">
        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048254">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14269:SF60">
        <property type="entry name" value="CARDIOLIPIN SYNTHASE (CMP-FORMING)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14269">
        <property type="entry name" value="CDP-DIACYLGLYCEROL--GLYCEROL-3-PHOSPHATE 3-PHOSPHATIDYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00379">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="12" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7726118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="326" mass="35856" checksum="A7879990ACEBE328" modified="2024-05-29" version="1">MAILRSLRNLTRKTSYTTTTRPSFLSTPNPTSFRTLKPSIFLSRSSSLLSPPIPNNFHCPLFLSFPPWKLLQSATPLYFQSDVVSVPKVLRPFDLIQEPKFPIKLGFVKSEGHVGDREGSEVLQSFVNWPNFISMTRLVSGPVLGWMIMQDMYLPAFIGLVVSGATDWLDGYVARKMGINSVVGSYLDPLADKVLIGCVALAMVERGLLHSGLVALVVIRDLALVGGAVYIRANHLGSKSRSWSEFFNLDGVRPQKVEPLMISKVNTCLQLALVTGALLQPEFGTQETGSYITYLSWLVAATTVGSTAAYGAQHLKSRSIFVRKNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G3I9</accession>
    <name>A0AAD5G3I9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_009496</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LOR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011386">
        <property type="protein sequence ID" value="KAI7727390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G3I9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.200">
        <property type="entry name" value="LURP1-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007612">
        <property type="entry name" value="LOR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038595">
        <property type="entry name" value="LOR_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025659">
        <property type="entry name" value="Tubby-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31087">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31087:SF60">
        <property type="entry name" value="PROTEIN LURP-ONE-RELATED 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04525">
        <property type="entry name" value="LOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54518">
        <property type="entry name" value="Tubby C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7727390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="212" mass="23630" checksum="4F084538F27A4CB5" modified="2024-05-29" version="1">MVIVEEGFAYENETHLTVMKTSLFFSGDGFVVYDSNGQLVFRVDSYSPDTRDTGELVLMDTNGRCLLNVRRKRPSLHQRWEGFIGDGWEGKKPIFSVRRSSIIGRSNMTVELFGDRTEEYQIEGQFAQRNCTIYDSSKEIMAEIRRKVDTTTNVTLGKDVFLLTLKPGFDGAFAMGLVLVLDQINGDDIGDDDGGAVDGDIVDPNTDCNVSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G440</accession>
    <name>A0AAD5G440_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_004460</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011500">
        <property type="protein sequence ID" value="KAI7726866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G440"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053197">
        <property type="entry name" value="F-box_SCFL_complex_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34223:SF118">
        <property type="entry name" value="F-BOX DOMAIN, LEUCINE-RICH REPEAT DOMAIN SUPERFAMILY, F-BOX-LIKE DOMAIN SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34223">
        <property type="entry name" value="OS11G0201299 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="29"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7726866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="331" mass="38204" checksum="3628A498448E3ABE" modified="2024-05-29" version="1">MAQRKSLRIRERAREMLNDDEDDDRISRLPKDVIHHIFSFMDARFVVQSGRLSRSWRNTWKSHPHLNFDISPSRDQTRGSKFSNFVHRFLSMRDEVTELSTINFRSNSINHSLLREIIIYATSHGTRNLKIEFSGVKQTSRGEFDLSLFRSRYLEHLFLSINVELKLNPSLTWDFPALTTLTIECVTFTLQLPNDGGTPKLSSFAYYGVARFSLTANDLLSLNTVNFQLPPSMAAFTGVMNYFSRSSPDVKHEELIVWFLLRERQLTDAFTPRQIGVAECKKIPVMNMVVTFLAAKKMLKLFWAEAIVWTACVLNRCPTKACTDVIPQEKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G4F8</accession>
    <name>A0AAD5G4F8_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_006367</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011220">
        <property type="protein sequence ID" value="KAI7728409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G4F8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7728409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="51" mass="5386" checksum="B3D2F881E860214E" modified="2024-05-29" version="1">MGCNGSVSAATHGVDEAVVDIGVPVVRLGFTATDGCECLVIPWCRWVFLAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G4H5</accession>
    <name>A0AAD5G4H5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Malectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_026223</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011288">
        <property type="protein sequence ID" value="KAI7727932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G4H5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.430:FF:000004">
        <property type="entry name" value="Putative leucine-rich repeat receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.430">
        <property type="entry name" value="Galactose-binding lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021720">
        <property type="entry name" value="Malectin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34081">
        <property type="entry name" value="MALECTIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34081:SF1">
        <property type="entry name" value="MALECTIN, LEUCINE-RICH REPEAT DOMAIN, L DOMAIN-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11721">
        <property type="entry name" value="Malectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Malectin" evidence="1">
        <location>
            <begin position="55"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF11721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7727932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="296" mass="32453" checksum="64D6844123B28D55" modified="2024-05-29" version="1">MLCNDLSYNNFTSQNSSDFSCQIRETNLFASYSTDNVSKEVSCLGNNICSTSSTSLYIDCGGNGVSVGNKDYEGDFVSGGASHFSSTDNRWGFSNTGHFLDDDQHDSYILTSSSQVPINNSELYMNARTSAISLTYYGFCMRNGSYNVTLHFAEIAFTDDTKFSSLGRRVFDIYIQGMLVEKDFDISDRAGGVGKAIVTTYTVNVTTSLEIRLYWAGKGTINVPTRGRYGPLISAISVDPCEGLKTLGVALSDNLEELALTNCDVIKEQNGFLVELAQNLKKIKILDLSLQSYSAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G528</accession>
    <name>A0AAD5G528_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SLC26A/SulP transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_023536</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011093">
        <property type="protein sequence ID" value="KAI7728994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G528"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011547">
        <property type="entry name" value="SLC26A/SulP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001902">
        <property type="entry name" value="SLC26A/SulP_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11814">
        <property type="entry name" value="SULFATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00916">
        <property type="entry name" value="Sulfate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="SLC26A/SulP transporter" evidence="5">
        <location>
            <begin position="92"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7728994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="138" mass="15753" checksum="3B24134B65544C09" modified="2024-05-29" version="1">MPDQDDKDHEMNWTSLTFQDGQTPNGPYIHKIGVPPKPNLLKEFKTTVKETFFPDDPLRTFKDQSRSRKIILGAQAIFPILEWGRSYNITKFRGDLIAGLTIASLCIPQDIGYSNLAYLKPQYGLCKCFLHLFVVQSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G608</accession>
    <name>A0AAD5G608_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Short-chain dehydrogenase TIC 32, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_019084</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011166">
        <property type="protein sequence ID" value="KAI7728713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G608"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05327">
        <property type="entry name" value="retinol-DH_like_SDR_c_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055280">
        <property type="entry name" value="TIC32"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48476">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE TIC 32, CHLOROPLASTIC-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48476:SF1">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE TIC 32, CHLOROPLASTIC-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7728713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="324" mass="35546" checksum="C74B9E2E7AE2EAD1" modified="2024-05-29" version="1">MGIIRLITGWPGPSGFGSATTAEQVTEGIDASHLTVVITGGASGIGLETARVLALRGAHVIIAARNMEAANDAKQNIIKNNQHAKVDILQLDLSCLKSVKAFADNFLALNIPLNILINNAGIMFCPYQLSQDGIEMQFATNHLGHFYLTKLLLDKMKSTASATGIEGRIVNVSSVAHLHTYKEGIKFDTINDKDSYSEKLAYGQSKLANLLHANELSRLLKEEGANVTVNSVHPGLIMTNLMKHSIKLMRIMNLLTYVLWKNVPQGAATTCYVAVHPNMKGVSGNYFLDCNEWPASDFARDPKMAKKLWDYSNHLLQSVIQHNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G705</accession>
    <name>A0AAD5G705_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_012381</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010931">
        <property type="protein sequence ID" value="KAI7729703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G705"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7729703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="183" mass="20594" checksum="9949ACBF4E7DBBD4" modified="2024-05-29" version="1" fragment="single">DFYNFLLDSGMDPVSMAATEAVRATPRKRYLRYLDSLTTSPDENGDLGLLMEGAIRKKLGIIPNDVNWGGQSDLVDELLNKGVNVTIYNGQLDVICSTKGTEAWVKKLKWDGLKTFLNTDRTPLYCGDDLITKGFTKSYRNLHFYWILEAGHFVPVDQPCIALEMVGNITRSSVSTNEDEYEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G9H6</accession>
    <name>A0AAD5G9H6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Leucine-rich repeat-containing N-terminal plant-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_004336</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010303">
        <property type="protein sequence ID" value="KAI7732118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G9H6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000041">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47988">
        <property type="entry name" value="SOMATIC EMBRYOGENESIS RECEPTOR KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042113265" description="Leucine-rich repeat-containing N-terminal plant-type domain-containing protein" evidence="7">
        <location>
            <begin position="27"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="8">
        <location>
            <begin position="29"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7732118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="203" mass="22968" checksum="BE4252FCD2A63249" modified="2024-05-29" version="1">MDRIPSLFSFSTLLLFILVLYKVSSAYGNAEGDALYALKIQLSDPNNVLQSWDPTLINPCSWNQVTCNNNNSVTRVINPSFCKVGIFPNRIHVNGFMLLATMRVVLLECKHMVIDPDLTNANLSGRLIPELGQLTNLEYLELYCNNISGKIPTELGELKNLVSLELYSNRLEGNIPDTLGNLKKLRFLYDFTSFRFSFNEISH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GD03</accession>
    <name>A0AAD5GD03_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ricin B lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_026692</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009155">
        <property type="protein sequence ID" value="KAI7736959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GD03"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042053345" description="Ricin B lectin domain-containing protein" evidence="1">
        <location>
            <begin position="26"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7736959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="224" mass="24583" checksum="06D9CB1B72D42450" modified="2024-05-29" version="1">MDIIIRTLCSIIILINLALPVHVGATTPLVLQSKRLCSQCSKCDTKTCPPSEAYPHMTALDNTLIAGALQSDYVNASDRGVYSVPNIVGGESAQYNAYFGWKSTSGSASGYHRFSNYMDKCSGGENYLTVSKQGKVSLQSLTSLESLAFADWKSIAPPKHLNHREYRFWVSRGTGKCLTMFGGNSKKRTVGVADCKFDGANKGQLFAFRFHYHNSFCCCGHHNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GD95</accession>
    <name>A0AAD5GD95_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF8040 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_004393</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008909">
        <property type="protein sequence ID" value="KAI7737997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GD95"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058353">
        <property type="entry name" value="DUF8040"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46929">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46929:SF4">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26138">
        <property type="entry name" value="DUF8040"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF8040" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF26138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7737997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="111" mass="12646" checksum="54784AA9ABE4C90D" modified="2024-05-29" version="1">MSEKAFMTLCSILKRDGGLRPTQRMSVEEHVATFLHISKPDAATLKTKKISNYNEMLELFARDRASGAQAETAKERNARMQKTDNINIESIPEDDFLAANDVMLESQKHYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GFQ5</accession>
    <name>A0AAD5GFQ5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Lethal giant larvae (Lgl)-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_008451</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat L(2)GL family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008822">
        <property type="protein sequence ID" value="KAI7738388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GFQ5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045159">
        <property type="term" value="F:myosin II binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15873">
        <property type="entry name" value="R-SNARE_STXBP5_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013905">
        <property type="entry name" value="Lgl_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10241">
        <property type="entry name" value="LETHAL 2 GIANT LARVAE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10241:SF27">
        <property type="entry name" value="TRANSDUCIN_WD40 REPEAT-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08596">
        <property type="entry name" value="Lgl_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lethal giant larvae (Lgl)-like C-terminal" evidence="3">
        <location>
            <begin position="539"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1018"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7738388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="1018" mass="112075" checksum="3E99923235AA0596" modified="2024-05-29" version="1" fragment="single">IVLKSFDYVLLAFDLRNLVEKASLKKPGGNADSLKSDDVNPRLIYHYGPPSGCNLLAYDPIQKILAMSTIDGRIKLFGQDNTQFVMESPETVPSKFMQFIHNQQFLININAKNQIEVWDIDTKYVGDSFGNVSILKFDKDTCNITQMQYRIPFAAAQGNSTEDLNDAAVMYILPQPAAESKRILIIYKNGFVTLWSIQDSKPVFTTGGTLIYSQTHDNKKVTAACWACPFGSKVVIGYNNGEILMWSVPHSVDSKTELSTDKALSGTQSGPLFKLNLGYKLDKTPIARLKWVYAEGKASRLYVLGASDFSSANSLQIVLINEQIESRTTKLGIASPEPCVDFEIVSSCSEQNKHKEDCFLVVGKSGHVYLYDDFSIEKYLLQSQSKSPPSLPKEVKVRLPYADSCITVAKFITDNPCMLSSADEDYRLLSKKIPAVFSFEAKQKDGSSSSGHSNGAINFWDASSPLLMPIVSFNQQSEDDQSLSGVPLTALCFDMEARLLVSGDQSGTVRIFKFKPEPYATESNFLSLQGSSKKGSNIIQSVKLVKVNGAVLSLITSHDTKHIAVGSEQGYVSVIDMTGPTLLYEKHIASELSTAVISLQFGMCSLHGFEKSVLVAATKDSSAWALESETGNILNTAAVHPKKPSKALFIQLLDEHGTSGRGSNVSKGGNSFDDGIVKDLLLFCSEKAVYVYSLPHVVQGVKKVCYKKKFHSSTCCWASTCCSDTGLVLVFTNGKIEIRSLPELSLLKATSIRGLALSTSKPNTLPDSSICASQNGDIVMVNGDQEVFIASVLLQREIFRHLDSATRVYDKDLIIPQGKQSDHVIHKEKKKGLFGSMIKGSKPKHELETEPESARESFEELSILFSVANFPSESGTRENLPTDEDEVELDIDDIDIDGPEEKPKGKTMMAALNKQKLTSKFNAFKGKLKEMNAKKEKVQVKEEPQDEKTGVDQIKKKYGFSSTGETSAAKMAQDKLGDNIRKLKGINLRTAEMQDNAQTFSSMAKEMLRTAENDRRTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GI07</accession>
    <name>A0AAD5GI07_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_029274</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008087">
        <property type="protein sequence ID" value="KAI7742014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GI07"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000051">
        <property type="entry name" value="Heterogeneous nuclear ribonucleoprotein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48033:SF4">
        <property type="entry name" value="OS08G0320100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48033">
        <property type="entry name" value="RNA-BINDING (RRM/RBD/RNP MOTIFS) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="15"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="103"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="177"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="184"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7742014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="350" mass="35996" checksum="7AFF668AFF8B0783" modified="2024-05-29" version="1" fragment="single">MASKRSDYGDGASPGKIFIGGLAKETTIDTFVKYFGKYGEITDSVIMKDRLTGRPRGFGFITYADPSVVDTVIAETHVISGKQVEIKRTIPKGSAESKDFKTKKIFVGGIPAAVTEDEFKEFFSKYGKVSEHEIIRDHVTKRPRGFGFIVFENEQSVDEILTNGNMIDMNGTQVEIKKAEPKKSSNSGINSYNREPKGRGYGGSYDGFDDSYGGYAGSGGGYGPPPYRSFGGPPSRFGDYGPYGPGAGEFGGRYGDYGGDYGGYRGGEPPLGGYSSRLSSYGGGGGGGYGGSGSGGYGGGYGGSGFGGGYGRGGPAGGGGYGGYGGPAGGGGYESGAGSGYGGAGDPSRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GJD8</accession>
    <name>A0AAD5GJD8_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_016826</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007405">
        <property type="protein sequence ID" value="KAI7744927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GJD8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7744927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="142" mass="15776" checksum="94A57FA56B4404E2" modified="2024-05-29" version="1" fragment="single">RTPPIQPSGQRYTTRLQPPARPVHHSLQPPPTTTCVNHQVRSRAALDLCFGVNPLPASLATASILILWSTHFTGYICYIQRCWINIKSVVSKSQRQTWAEAVLRLDNGGSLRHFPLLLGFDPDVGEYMSSDNGSCLSYLIST</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GKI9</accession>
    <name>A0AAD5GKI9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PUM-HD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_032658</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007835">
        <property type="protein sequence ID" value="KAI7742993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GKI9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000818">
        <property type="entry name" value="Pumilio homolog 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012959">
        <property type="entry name" value="CPL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033133">
        <property type="entry name" value="PUM-HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040059">
        <property type="entry name" value="PUM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001313">
        <property type="entry name" value="Pumilio_RNA-bd_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13389">
        <property type="entry name" value="PUMILIO HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13389:SF0">
        <property type="entry name" value="PUMILIO HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08144">
        <property type="entry name" value="CPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22493">
        <property type="entry name" value="PUF_NOP9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00025">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50302">
        <property type="entry name" value="PUM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50303">
        <property type="entry name" value="PUM_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="PUM-HD" evidence="6">
        <location>
            <begin position="91"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="4">
        <location>
            <begin position="155"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="4">
        <location>
            <begin position="191"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="4">
        <location>
            <begin position="342"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="426"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="42"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="442"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7742993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="633" mass="70709" checksum="C2784C1970FED2C8" modified="2024-05-29" version="1">MAFKNQPNKRKKLTGGTDAAKPTPNKKLKTSSNDQQNKGFKKKFDSKKPKFDKPDTTNNAPQTKRELRIQAKELAEARKKKRKPHYTLEQELASLWEKMRRRNIGKEDRSKLVSEALRKMKGKISEIAGSHVSCRVLQTCAKYCTQDERNAVYEELKPHFLTLACNTYAVHLITKLLDNASKEQLASFISSLRGHVAPLLRHMVGSVVVEHAYQLGNASQKQSLLMELYSTELQLFKDLALVKERRLVDIISKLNLQKSAVVRHMSAVVQPILEKGIVDHSILHKALVEYLTIADKSSAAEVIQHLSSALLVRMIHTKDGSKIGILCIKHGSAQERKKIIKGMKGHVSKIALDRFGSMVLTCILSVVDDTQLIKKIIIRELQPVLKELICDKNARRPLLQLLHPNCSRYLSPDDLSLLDSSIPSLITKGGSEPETETKSSEVNDDDVDMSDAEDNVKSVAGGKKDPTLRRKELLVDSGLAESLIDACCESAEELLRSNHGKEVLFEVASGGADSILHPHLDEKLDSLHEAIASRVSLPKCEGQEEEHLLENFHSSRTIRKLILDCPSFASTLWEKTFKGNCKTWAQGHSAKVVSAYLETSDPKVLKLAKKELKPLLDQGLLKLPEQSQPKKEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GKT1</accession>
    <name>A0AAD5GKT1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_003596</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007765">
        <property type="protein sequence ID" value="KAI7743248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GKT1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7743248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="85" mass="9410" checksum="64F2A7EBEFEB5113" modified="2024-05-29" version="1" fragment="single">SLSLSHSLSLSKTTTISAALSSTEPPSPSANEYRRLERKPPVTAALFAANTIIYLRPSFLHQVIPPVDDVYFNAYLILKMVSRTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GLS1</accession>
    <name>A0AAD5GLS1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_021159</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007558">
        <property type="protein sequence ID" value="KAI7744253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GLS1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34553:SF4">
        <property type="entry name" value="G1_S-SPECIFIC CYCLIN-E PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34553">
        <property type="entry name" value="OS05G0597400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7744253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="125" mass="14268" checksum="8CA02FC6AD195A6B" modified="2024-05-29" version="1">MASYALNGSVIVAFCSRLDRTLFGFIVFQVAWKDVRCINYLNELQTDTSLAIEAKYMKRWEFDSINQAVKRINTWDSTQTRSIGRASQLHASPAAVFNHPVRQIKKARVARHSLQKIRHCGTLGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GMA1</accession>
    <name>A0AAD5GMA1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcription initiation factor TFIID subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_031829</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007031">
        <property type="protein sequence ID" value="KAI7746139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GMA1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1730:FF:000015">
        <property type="entry name" value="Transcription initiation factor TFIID subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037813">
        <property type="entry name" value="TAF2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15137">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15137:SF9">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7746139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="394" mass="44772" checksum="CB7B619A7F1581B7" modified="2024-05-29" version="1" fragment="single">MAKPRKHKTEDQKADNNSEAVVKHQKLCLAIDLQKRRIYGYTELEVAVPDNGIVGLHADNLMIECVTVDGEPARFEIFPHYQQMDSDDRWCSVSSANSAADAAGSVYVSCLERELVPNLLIMCSNEAAKPVPEQLEQTVQENGTQTSSESKQNVKLIHIDYWVEKIETGIHIEKDVMHTNNQIRRARCWFPCMDDSLQRCCFDLEFTVANNLVAASTGTLMYQVLTNDDPPRKTYIYKMNVPVAAQWVSLAVAPFEIFPDRHNNLITHICLSHNLPKLRNTVGFLYSAYSHYEHYLSTKFPFGSYTQVFIDPEMAVSSLSLGASMNIFTSQILFDEKIIDQTIDTRIKLAYGLARQWFGVYITAEAPNDEWLLDGLAWFLTDSFIKQFMGNNEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GRI7</accession>
    <name>A0AAD5GRI7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_027617</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006377">
        <property type="protein sequence ID" value="KAI7749383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GRI7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47711">
        <property type="entry name" value="PROTEIN PLASTID TRANSCRIPTIONALLY ACTIVE 16, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47711:SF2">
        <property type="entry name" value="PROTEIN PLASTID TRANSCRIPTIONALLY ACTIVE 16, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13460">
        <property type="entry name" value="NAD_binding_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-binding" evidence="2">
        <location>
            <begin position="31"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="329"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="388"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="329"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="395"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7749383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="441" mass="47670" checksum="1EBDE92B55E7453F" modified="2024-05-29" version="1">MRALIPVVSKTSSSGTVFGKRKDPNTVFVAGGTGLAGVRIVERLIRDGFGVRAGVPELGAAQELARLAVDYKVISTEESKRLNAVESTFQDPEAIAKAIGNATKAVVTIGPCENGPTTELTTSEALQLIQGAQLAGVSHVIIIYETSPPSFTSTNNVLDGISMFFNNLFSNTQPLTFKEFVDGLIATDVNYTLIKTRMTDDNASESSYNFVVSAEGITRENDYKVARSQIASLVADVFSNTEVAENKPDPFSLKHNRFIHVVIPEDGRRKAYAEANAKAKAEEAMLAAEKATKLAENEVKKLDPQDDFQERATIARKSFTDLVDRFKTVTKATEEPEPTSKDGSDQEPDGAPGLARSWEKLSAGFASAVKNNPIELPKVQIATVRGQAKAKNLTPKKAVVKKPATAKKAPSAVQPKPKESKTDVKKIFGGMFQQETIYVDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GRQ9</accession>
    <name>A0AAD5GRQ9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_016621</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006317">
        <property type="protein sequence ID" value="KAI7749638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GRQ9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7749638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="74" mass="8393" checksum="AB0065B688E44BB5" modified="2024-05-29" version="1">MPPPVQNPFMVPQTMISPLHIGTTSPTIHNRLPVNTTVPFNDPHNVLLAQPAGVQHINMDMYNNMVEFYRQQVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GTE3</accession>
    <name>A0AAD5GTE3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_021312</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005949">
        <property type="protein sequence ID" value="KAI7751243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GTE3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7751243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="95" mass="11151" checksum="CABDE010666D3D27" modified="2024-05-29" version="1">MTVVFSRFDGAVDSGLLRRRSRCFRFLTAHMLSDHRPMMVVGIYFALRHWMQEYDLILRVVDLKNMLSGQDSSESYFLNMIMKNEIPAAKRGVIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BKA5</accession>
    <name>A0AAD5BKA5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Copine C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_025682</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012218">
        <property type="protein sequence ID" value="KAI7724454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BKA5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071277">
        <property type="term" value="P:cellular response to calcium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045052">
        <property type="entry name" value="Copine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010734">
        <property type="entry name" value="Copine_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10857:SF106">
        <property type="entry name" value="C2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10857">
        <property type="entry name" value="COPINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07002">
        <property type="entry name" value="Copine"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Copine C-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="Copine C-terminal" evidence="1">
        <location>
            <begin position="71"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7724454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="131" mass="14357" checksum="18C17EAB3C9A31EB" modified="2024-05-29" version="1" fragment="single">DGELTDQQETIDALVTASDLPLSILIVGVGGADFKELEVLNYFHAFLLLCRLSACHEYHTKPSGKKSMICILDADKGEKLRSSTGRVASRDIVQFVPFRDIQGGGMSVVQSLLAELPSQFLTYMRNNGIQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BM54</accession>
    <name>A0AAD5BM54_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein TIFY</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Jasmonate ZIM domain-containing protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_004606</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Repressor of jasmonate responses.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="2">The jas domain is required for interaction with COI1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the TIFY/JAZ family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012171">
        <property type="protein sequence ID" value="KAI7724729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BM54"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031347">
        <property type="term" value="P:regulation of defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000022">
        <property type="term" value="P:regulation of jasmonic acid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009611">
        <property type="term" value="P:response to wounding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018467">
        <property type="entry name" value="CCT_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040390">
        <property type="entry name" value="TIFY/JAZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010399">
        <property type="entry name" value="Tify_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33077">
        <property type="entry name" value="PROTEIN TIFY 4A-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33077:SF5">
        <property type="entry name" value="PROTEIN TIFY 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09425">
        <property type="entry name" value="Jas_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06200">
        <property type="entry name" value="tify"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00979">
        <property type="entry name" value="TIFY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51320">
        <property type="entry name" value="TIFY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1184">Jasmonic acid signaling pathway</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tify" evidence="3">
        <location>
            <begin position="85"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU369065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7724729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="187" mass="20721" checksum="64745FD826D744DF" modified="2024-05-29" version="1">MSESSVIHEIDFFRLKEQSEESYPKKLSPRRNIRDVISKINPELLKPAVASAFANNRFVGNKSYASHPPLQTLPVYNHECSSVNTSTTAVPLTIIYNGVVTVFDVSPSHADNIMKLADNVNPVSKPVAESTVAVKHENNQNPSIGGVLNKDLPLSRKKSLKRFLEKRKERQVSISPYANSQGNASLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BMV3</accession>
    <name>A0AAD5BMV3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Prolyl 4-hydroxylase alpha subunit domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_027683</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-prolyl-[collagen] + 2-oxoglutarate + O2 = trans-4-hydroxy-L-prolyl-[collagen] + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:18945"/>
            <dbReference type="Rhea" id="RHEA-COMP:11676"/>
            <dbReference type="Rhea" id="RHEA-COMP:11680"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:50342"/>
            <dbReference type="ChEBI" id="CHEBI:61965"/>
            <dbReference type="EC" id="1.14.11.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012010">
        <property type="protein sequence ID" value="KAI7725198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BMV3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031418">
        <property type="term" value="F:L-ascorbic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004656">
        <property type="term" value="F:procollagen-proline 4-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045054">
        <property type="entry name" value="P4HA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006620">
        <property type="entry name" value="Pro_4_hyd_alph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044862">
        <property type="entry name" value="Pro_4_hyd_alph_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869:SF123">
        <property type="entry name" value="PROLYL 4-HYDROXYLASE 10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869">
        <property type="entry name" value="PROLYL 4-HYDROXYLASE ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13640">
        <property type="entry name" value="2OG-FeII_Oxy_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00702">
        <property type="entry name" value="P4Hc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <feature type="domain" description="Prolyl 4-hydroxylase alpha subunit" evidence="9">
        <location>
            <begin position="1"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7725198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="145" mass="16390" checksum="E3D0FBFF0218B841" modified="2024-05-29" version="1" fragment="single">YCVFTWKSQEECEYLINMAKPHMVKSTVVDSDTGKSKDSRVRTSSGTFLARGRDKTVRTIEKRIADFTFLPVEHGEGLQVLHYEVGQKYEPHYDYFQDEYNTQNGGQRMATVLMYLSDVEEGGETVAARLSRGTSGPLQNGYAST</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BPT7</accession>
    <name>A0AAD5BPT7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_027569</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011403">
        <property type="protein sequence ID" value="KAI7727297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BPT7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050232">
        <property type="entry name" value="FBL13/AtMIF1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31900">
        <property type="entry name" value="F-BOX/RNI SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31900:SF31">
        <property type="entry name" value="F-BOX_LRR-REPEAT PROTEIN 13-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7727297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="220" mass="25044" checksum="B99422336E7148A4" modified="2024-05-29" version="1" fragment="single">SGGELQVVPTQALTKLRIGLGLPSLVWECPCLVNLPSLKTLDIAVFDDPFVNAFELIRGCPVLERSKHLEVIKNKAVEEEYVFNIPTLKRLKLTWRRAFPTSVTKKVVLNVPNLEYLFVGGISGSLFVMEDLSSLVEVSVSFDYIRSDDMCWEFLKGISGVKSLSIEKFPFTSALPIFPNMKHLEFKRYWYPKIVLQHLESCPELKHLCIHMLEAQNSMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BPU8</accession>
    <name>A0AAD5BPU8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PDZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_008592</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011726">
        <property type="protein sequence ID" value="KAI7726248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BPU8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF047558">
        <property type="entry name" value="TPR_END_plus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47661">
        <property type="entry name" value="PHOSPHOGLUCAN PHOSPHATASE LSF1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47661:SF3">
        <property type="entry name" value="PROTEIN CONTAINING PDZ DOMAIN, A K-BOX DOMAIN, AND A TPR REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="2">
        <location>
            <begin position="85"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7726248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="323" mass="36492" checksum="BD2908B3C01C9F57" modified="2024-05-29" version="1">MATAPNSYPSLCSSPPLPRTHIQPTKQSLSFVTSTKNSLKLRDFIRLSVSGSGKQSGLMVRASAEETPTEEEKYEEYEVEIEQPFGLKFAKGRDGGTYIDAIAPGGSADKINMFTVGDRVLATSAMFGTEIWPAAEYGRTMYTIRQRIGPLYMRMQKRYGKREDMGELTEKEIIRAERNSGVVSSKVREIQMQNYLRKKEQKETRARDLREGLQLYKSGKYDDALEKFESVLGSKPELDEASVASYNVACCYSKLDQIQAGISALKDALQAGFEDFKRIRTDPDLENLRKSEQFEPLIKQYDESFINENALNAIKSLFGFNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BQK6</accession>
    <name>A0AAD5BQK6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">methionine adenosyltransferase</fullName>
            <ecNumber evidence="10">2.5.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">M8C21_012707</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="23">AA19_3_7</strain>
            <tissue evidence="23">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The reaction comprises two steps that are both catalyzed by the same enzyme: formation of S-adenosylmethionine (AdoMet) and triphosphate, and subsequent hydrolysis of the triphosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-methionine + ATP + H2O = S-adenosyl-L-methionine + phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21080"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="2.5.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="7">Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the AdoMet synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.6" evidence="10"/>
    <dbReference type="EMBL" id="JAMZMK010011591">
        <property type="protein sequence ID" value="KAI7726726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BQK6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004478">
        <property type="term" value="F:methionine adenosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006556">
        <property type="term" value="P:S-adenosylmethionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.10:FF:000001">
        <property type="entry name" value="S-adenosylmethionine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022631">
        <property type="entry name" value="ADOMET_SYNTHASE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022630">
        <property type="entry name" value="S-AdoMet_synt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022628">
        <property type="entry name" value="S-AdoMet_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002133">
        <property type="entry name" value="S-AdoMet_synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022636">
        <property type="entry name" value="S-AdoMet_synthetase_sfam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11964">
        <property type="entry name" value="S-ADENOSYLMETHIONINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02773">
        <property type="entry name" value="S-AdoMet_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00438">
        <property type="entry name" value="S-AdoMet_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55973">
        <property type="entry name" value="S-adenosylmethionine synthetase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00376">
        <property type="entry name" value="ADOMET_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="19" id="KW-0170">Cobalt</keyword>
    <keyword evidence="11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="18" id="KW-0630">Potassium</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="S-adenosylmethionine synthetase N-terminal" evidence="21">
        <location>
            <begin position="4"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="S-adenosylmethionine synthetase C-terminal" evidence="22">
        <location>
            <begin position="155"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00001958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00003583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00005224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00009685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00012828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF00438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF02773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="KAI7726726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="257" mass="28190" checksum="788AD0298547631D" modified="2024-05-29" version="1">METFLFTSESVNEGHPDKLCDQVSDAILDACLEQDPESKVACETCTKTNMVMVFGEITTKAVVDYEKIVRDTCRNIGFTSADVGLDADKCNVLVNIEQQSPDIAQGVHGHLTKRPEEIGAGDQGHMFGYATDETPELMPLTHVLATKLGAKLTDAAKSVVASGLARRCIVQVSYAIGVAGPLSVFVDTYKTGTIPDSDYILALIKENFDFRPGMMAINLDLTRGRNYRYQKTAAYGHFGREDPDFTWETPKILKPKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BQY2</accession>
    <name>A0AAD5BQY2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">RBR-type E3 ubiquitin transferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_006065</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RBR family. Ariadne subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011277">
        <property type="protein sequence ID" value="KAI7727998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BQY2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22583">
        <property type="entry name" value="Rcat_RBR_ARI7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.380">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031127">
        <property type="entry name" value="E3_UB_ligase_RBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036443">
        <property type="entry name" value="Znf_RanBP2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11685">
        <property type="entry name" value="RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22191">
        <property type="entry name" value="IBR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00547">
        <property type="entry name" value="ZnF_RBZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90209">
        <property type="entry name" value="Ran binding protein zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01358">
        <property type="entry name" value="ZF_RANBP2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50199">
        <property type="entry name" value="ZF_RANBP2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="11">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="RanBP2-type" evidence="10">
        <location>
            <begin position="296"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="259"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="260"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7727998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="323" mass="36850" checksum="52E99B61051CFEED" modified="2024-05-29" version="1">MNFRILANSKPCPKCKRPIEKNQGCMHMTCNPPCKYEFCWLCLGQWSDHGERTGGFYACNRYESAKQEGAYDESERRREMAKNSLERYTHYYERWATNQSSRQKALADLHQMQTVHGLHFVPGSITGEITPWHFDALAKLSEKQCQPETNLKFIIDAWLQIVECRRVLKWTYAYGYYLPEKEHAKRQFFEYLQGEAEAGLERLHQCAEKELNTYLNEDGSQDDFNNFRTKLAGLTSVTRNYFENLVRALENGLADVDAHGASSKTSSSKNAAGTSKSKGTKGKGSTRGGATSRTDDPSGWSCDQCTYMNPKTVTSCQMCHNSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BS03</accession>
    <name>A0AAD5BS03_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_032611</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011252">
        <property type="protein sequence ID" value="KAI7728219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BS03"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7728219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="106" mass="12416" checksum="7A0ADC2A34012206" modified="2024-05-29" version="1" fragment="single">DEGPVNPVFAASFFQIRAIDDFQSQERREQQFWATEKREMVEGEKKEEDEKIHYNYSLHFHHRRINLVPQVRLETSASVGPTLKMASSNSPILKNANWEAKPNQFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BS40</accession>
    <name>A0AAD5BS40_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosome assembly factor mrt4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_027045</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 6">Component of the ribosome assembly machinery. Nuclear paralog of the ribosomal protein P0, it binds pre-60S subunits at an early stage of assembly in the nucleolus, and is replaced by P0 in cytoplasmic pre-60S subunits and mature 80S ribosomes.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Ribosomal protein P0 is the functional equivalent of E.coli protein L10.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Associates with the pre-60S ribosomal particle.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
            <location evidence="6">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the universal ribosomal protein uL10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011212">
        <property type="protein sequence ID" value="KAI7728460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BS40"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000956">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05796">
        <property type="entry name" value="Ribosomal_P0_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1730:FF:000005">
        <property type="entry name" value="Ribosome assembly factor mrt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.105.20:FF:000004">
        <property type="entry name" value="Ribosome assembly factor mrt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.105.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033867">
        <property type="entry name" value="Mrt4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001790">
        <property type="entry name" value="Ribosomal_uL10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040637">
        <property type="entry name" value="Ribosomal_uL10-like_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043164">
        <property type="entry name" value="Ribosomal_uL10-like_insert_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043141">
        <property type="entry name" value="Ribosomal_uL10-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051742">
        <property type="entry name" value="Ribosome_Assembly_uL10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45841:SF1">
        <property type="entry name" value="MRNA TURNOVER PROTEIN 4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45841">
        <property type="entry name" value="MRNA TURNOVER PROTEIN 4 MRTO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00466">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17777">
        <property type="entry name" value="RL10P_insert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160369">
        <property type="entry name" value="Ribosomal protein L10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL10-like insertion" evidence="8">
        <location>
            <begin position="126"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU364039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7728460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="229" mass="26166" checksum="A549F084C536C4A8" modified="2024-05-29" version="1">MPKSKRDRAVTLSKTKKKGREHKESIVSSIRDAVEKYNSIYVFSFENMRNLKFKQFREQLKSTSRFFLGSNKVMQVALGRSDSDEMRPGLHKVSKLLRGDSGLCLTNMPVEEAQRIFGEYEDYDYARTGSIATETVELKEGPLDQFTHEMEPFLRKQGMPVRLNKGVVELVGDFVVCEEGNPISPEAARILRSMGMKMATFKLNLICRWSPEDFEVYQEGLQGSDIESS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BT32</accession>
    <name>A0AAD5BT32_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MYND-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_000952</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011097">
        <property type="protein sequence ID" value="KAI7728970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BT32"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09917">
        <property type="entry name" value="F-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="6.10.140.2220:FF:000033">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057136">
        <property type="entry name" value="At2g35280_TPR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044508">
        <property type="entry name" value="At5g50450/At1g67340-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002893">
        <property type="entry name" value="Znf_MYND"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46758">
        <property type="entry name" value="MYND DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46758:SF16">
        <property type="entry name" value="ZINC FINGER, MYND-TYPE, TETRATRICOPEPTIDE-LIKE HELICAL DOMAIN PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23310">
        <property type="entry name" value="TPR_27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01753">
        <property type="entry name" value="zf-MYND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144232">
        <property type="entry name" value="HIT/MYND zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50865">
        <property type="entry name" value="ZF_MYND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MYND-type" evidence="5">
        <location>
            <begin position="278"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7728970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="341" mass="37649" checksum="9C58E486BFF08334" modified="2024-05-29" version="1">MRTRRRSYYEGANNMSGPHHKRLKLFSTLDYLDSLPDDIVLLILANLCSTATSPADFITVLSTCKRLNSLGVHSLVLSKASLNCLAVNPKNWSESSHRFLKRCSDAGSAEASYTLGMIRFYCLQNRTSGASLMAKAAIRSHAPALYSLAVIQFNGSGGMKNDKDLRAGVALCARAAFLGHVDALRELGHCLQDGYGVPKNVTHGRRLLIQANARELARVFSLSSNQLLLTGSVCPLFSDFGCNVPAPDPHHANRFLVGWFSDKLQLELESPDTRLCSYGGCGRMETRRHEFRRCSVCGDVNYCSRACQARDWKLHHNMTCRVGLEGVGFNANVNDTMVDER</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BTQ4</accession>
    <name>A0AAD5BTQ4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_001323</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011023">
        <property type="protein sequence ID" value="KAI7729292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BTQ4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7729292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="133" mass="15479" checksum="2BB0406DA2983BDE" modified="2024-05-29" version="1" fragment="single">NKIEYSEKLERERSRDERQIRGDDRLAAVCAQLIGPHLSYTTRQAFLPFLYSPDDYRMTESVQAPHPYHYMYQHLSSGINYDFFCLGTNGFYTLCKTNVGNTVAQRRRQNADVRSIDMVILGGRPSILLFQHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BXG5</accession>
    <name>A0AAD5BXG5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_030810</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DnaX/STICHEL family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010513">
        <property type="protein sequence ID" value="KAI7731210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BXG5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009360">
        <property type="term" value="C:DNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005663">
        <property type="term" value="C:DNA replication factor C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003689">
        <property type="term" value="F:DNA clamp loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18137">
        <property type="entry name" value="HLD_clamp_pol_III_gamma_tau"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000013">
        <property type="entry name" value="DNA polymerase III subunit gamma/tau"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012763">
        <property type="entry name" value="DNA_pol_III_sug/sutau_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050238">
        <property type="entry name" value="DNA_Rep/Repair_Clamp_Loader"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054506">
        <property type="entry name" value="DnaA_N-like_STI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045085">
        <property type="entry name" value="HLD_clamp_pol_III_gamma_tau"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02397">
        <property type="entry name" value="dnaX_nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669:SF46">
        <property type="entry name" value="PROTEIN STICHEL-LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669">
        <property type="entry name" value="REPLICATION FACTOR C / DNA POLYMERASE III GAMMA-TAU SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13177">
        <property type="entry name" value="DNA_pol3_delta2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23007">
        <property type="entry name" value="DnaA_N-like_STI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22608">
        <property type="entry name" value="DNAX_ATPase_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="post-AAA+ oligomerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="9">
        <location>
            <begin position="489"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="143"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="279"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="363"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="802"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="744"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="159"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="178"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="194"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="287"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="385"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="813"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1087"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7731210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="1087" mass="120297" checksum="0F182A38A6F08D3C" modified="2024-05-29" version="1" fragment="single">TIVSINNILHHLHLSSLSLSRATLSLSRPPSSVSVSNEPTLIRAATVKESTLVKLIFNEENGSLASLPLIQVLRKKMVYLNRIMTRVVRDRILKDANGDISDHLRNHIHLTNCIHLKNHMHKNSPITTDRSLIRDLVVLQRSRSLRDPSMSPPPFQSPSGGGGGGGGGGGGDGGARRSVGLSGNSSSNVGTGDVIHRRIKKEESSRRSFNDDSPLDAANLARDIYPGSSDSKGKQKGRKSIKSRQDDHVKTLLQQLNDGPIESDNVASSHNRFHGGVEVESSLHSHGVSRAKRRKFKGGRRASRAPKNEVSMASNFVPHVDFDGQQNVSGAPKNGCGIPFNWSRIHDRGKSFLDIAGKSLSCGLSDSRSKRGGQRDSGQIPVLSDDSTSSTNGESLPLLLDGSQGSRENPAAWVHDYSGELGIYADNLLNGGTDSDLVSEARSGHQKRARDNTRHQNLTQKYTPRTFRDLVGQNLAVQALSNAIAKKKVGLLYVFYGPHGTGKTSCARIFARALTCQSLDHPKPCGYCNSCVAHDMGKNRSVREVGPMNNLGYKRIMELIENMIVSHIPSQYRVFIIDDCDSLPPNCWSAISKVIDRAPRRMVFLLVNSSLDVLPHIIISRCQKFFFPKLKDADIIYTLQWIATKEDFEIDKDALKLIASRSDGSLRDAEMTLEQLSLLGQRISVSLVQELVGLISDDKLVDLLDLALSADTVNTVKHLREIMESGVEPLALMSQLATVITDILAVSKEDMERLRLALKTLSEAEKQLRTSNDKLTWLTAALLQLAPDQQYVLPTSSVDTSIHHSPMGLSNGGRRDGPRKSNFEHGETTGIQRNILKNVGNSSKMKERLYQQEIEEIWFEVLENIPINSIKEFLYHEGKLTSVSFGAAPTVQLNFSSHVTKSKAEKFKTHILKAFEHVLGSPVTIEIRSESRKETGESQEDLLYANRSEIIEIEEPKAKLRIDNNNNTIDNTEATTSHNNSNIGFQRRLGEQSQSLSLVKGKVSLANVIQQAESHRNGWSTRKAVSIAEKLEQENLRMEPRSRSLLCWKASRVARRKTKKAPGFAEIHVLWKVSFNEITEVTQKCLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BY55</accession>
    <name>A0AAD5BY55_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_011495</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010453">
        <property type="protein sequence ID" value="KAI7731384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BY55"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00018">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.730.10:FF:000001">
        <property type="entry name" value="Ethylene-responsive transcription factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.730.10">
        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001471">
        <property type="entry name" value="AP2/ERF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050913">
        <property type="entry name" value="AP2/ERF_ERF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31194:SF140">
        <property type="entry name" value="ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR CRF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31194">
        <property type="entry name" value="SHN SHINE , DNA BINDING / TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00847">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00367">
        <property type="entry name" value="ETHRSPELEMNT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00380">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51032">
        <property type="entry name" value="AP2_ERF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0611">Plant defense</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="7">
        <location>
            <begin position="86"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7731384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="196" mass="21599" checksum="D33D4A3B6554B838" modified="2024-05-29" version="1">MNTLFQTLPYLPANPNSIEYVNPSLETQGLSEQDHPAVLEGIAAVVGEHVLFGKSGIMSNKETDYVDVKKETKFGSVSNSGGVEKNYRGVRKRPWGRWSAEIRDKIGRCRHWLGTFDSAEEAARAYDAAARRLRGSKARTNFEIPSVFPLEMSLTTEVKKRKPDGCSSSSSSSNKCHVVTSVGQLFSTTGPMAKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BYC0</accession>
    <name>A0AAD5BYC0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box/LRR-repeat protein 15/At3g58940/PEG3-like LRR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_004263</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010349">
        <property type="protein sequence ID" value="KAI7731973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BYC0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000648">
        <property type="entry name" value="F-box/LRR-repeat protein 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055312">
        <property type="entry name" value="FBL15-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055411">
        <property type="entry name" value="LRR_FXL15/At3g58940/PEG3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34709:SF57">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34709">
        <property type="entry name" value="OS10G0396666 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24758">
        <property type="entry name" value="LRR_At5g56370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box/LRR-repeat protein 15/At3g58940/PEG3-like LRR" evidence="1">
        <location>
            <begin position="498"/>
            <end position="607"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF24758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7731973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="716" mass="78325" checksum="7B433C72347C0FC2" modified="2024-05-29" version="1">MCHRYPNATHVNINGSPAIHTLVMQAISSLRNLEYLTLGKGQLGETFFQALTDCTMLKSLIVTDAILGNGIQEIPIYHDSLQHIQIVKCRVVRISVRCPQLQTLSLKRSSMAHAVLNCPLLHDLDIASCHKLLDAAIRSAVTSCPLLESLDMSNCSCVSDETLREISITCGNLHILNASYCPNISLESVRLPMLTVLKLHSCEGITSASMAAIAYSYMLEVLELDNCSLLTSVFLDLPRLQNIRLVHCRKFVDLSLRSLALLSIKVSNCPSLQRINIVSNSLQKLVLQKQESLSCLELQCQSLQEVDLTDCESLTNSICEVFSDGSGCPMLKSLILDNCESLTAVEFCSNSLTSLSLAGCRGITSLELTCPYLEQVSLDGCDHLERAICSPVGLRSLNLGICPKLNTLKIEAPTMVLLELKGCGVLSEASINCPLLTSLDASFCSQLKDDCLSATTASCPLIESLILMSCTSVGSDGLASLRWLPNLTSLDLSYTFLTNLQPVFDSCLQLKVLKLQACKYLTDSSLEPLYKEGALPTLRELDLSYGSLCQASIEELLACCTHLTHVSLNGCVNMHDLNWSFNVDRVYQCFLQNLNCVGCPNIKKVLIPSIAHFSNLSSLNMSLSAYLKEVDVTCSNLCFLNLSSLEILKLECPRLTSLFLQSCNISEAAVESAISRCHMLETLDVRFCQKISPASVGKLRTACPSLKRIFSSMVPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C0C1</accession>
    <name>A0AAD5C0C1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_029405</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010153">
        <property type="protein sequence ID" value="KAI7732802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C0C1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041266">
        <property type="entry name" value="EDS1_EP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002921">
        <property type="entry name" value="Fungal_lipase-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044603">
        <property type="entry name" value="SAG101-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46898:SF3">
        <property type="entry name" value="FUNGAL LIPASE-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46898">
        <property type="entry name" value="SENESCENCE-ASSOCIATED CARBOXYLESTERASE 101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18117">
        <property type="entry name" value="EDS1_EP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01764">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0611">Plant defense</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fungal lipase-type" evidence="7">
        <location>
            <begin position="48"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="EDS1 EP" evidence="8">
        <location>
            <begin position="331"/>
            <end position="537"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF18117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7732802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="550" mass="63096" checksum="0790616B027E972B" modified="2024-05-29" version="1">MRQTMFSSEVELGKFLGSIGIIPEAYQAILETDLTYELHTTRSGISVLAFKCSSDYNNRFLKGEFNLVPSDGLQVVDFIRTKVNSSFSINQAAVELFQQLGDDLKELENKHKNSSLIITGSGLGGYLAILSTLRLHHAIDVEESNGSRNTKRPICITFGCPLVGDVALQRAIAERPQWKSSFLNVVVSKDPVATFFSSNTPYKPFGTFLFCVESGGHTAFEDPDSILPVLDAMKLLNVGNLQSYDYTNVLNSIRRKVLYRGSSELDESNVSSLRAGIMLQLKEVDVLSDITNDEIGKMEKKQADMIKKKNLVSAYDPTKKLNEMKISLTYMEWYMKTTKSKGGYYDSYKNPKSKEEQERQNEIVKHRVRLNLYWKKTVEDKDRMPQKEGAKLRKRWLFGGTNYRRIVEPLDIAEYYKKGNINYIENRPDHYKLLEKWSDEDRRNGRNTSEEKRNKAASLTEDSCFWAHVEEAFISLRELVNGGSANSEQKLEEFEAYMMRAINNYLLSPDVFVEGSSLMKWWDEYKAHKGYGYASEFAEYMTNESYRSYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C142</accession>
    <name>A0AAD5C142_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Hexosyltransferase</fullName>
            <ecNumber evidence="5">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_022525</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1 5">Glycan metabolism; pectin biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Golgi apparatus membrane</location>
            <topology evidence="5">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the glycosyltransferase 8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="5"/>
    <dbReference type="EMBL" id="JAMZMK010010093">
        <property type="protein sequence ID" value="KAI7733070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C142"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047262">
        <property type="term" value="F:polygalacturonate 4-alpha-galacturonosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06429">
        <property type="entry name" value="GT8_like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029993">
        <property type="entry name" value="GAUT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002495">
        <property type="entry name" value="Glyco_trans_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32116">
        <property type="entry name" value="GALACTURONOSYLTRANSFERASE 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32116:SF7">
        <property type="entry name" value="GALACTURONOSYLTRANSFERASE 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25557">
        <property type="entry name" value="GAUT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01501">
        <property type="entry name" value="Glyco_transf_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="3 5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="154"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="89"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="157"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7733070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="610" mass="70330" checksum="42DB0E486786A80C" modified="2024-05-29" version="1">MKMKMHTWLRKPVLALLFITVLAPIVLYTDKVSTFSSYDFVQDPSTLSLNRDVTDVRPLNLLPQELSVAQEEPSADVYVENITTSVHVSDGERSRKTRQLNEESKDIGTDVSADEDNLIREVTEGENENRLELDENEDHVKVKGAEHSMIAAAQAETKPEAQKSTKTKTEKPNERVLADSRVRYLKDQLIRARVYLSLSSTRNNVQFNRELRARMKDVQRALGDASKDADLPRNAYDKLKAMDQTLAKGKQMQDDCTAIVKKLRAIIHSTEEQLRVHQKQALFLTHLTAKTVPKGLHCLPLRLSTEYYSLNSSAQQFPNQEKLDDPKLFHYALFSDNVLATAVVVNSTVTNAKEPWKHVFHIVTDKLNYAAMRMWFLTNPPGKATIQVQNIDEFTWLNASYSPVLKQLASQNMIDYYFKTHKAVEPDSNLKFRNPKYLSMMNHLRGLWSIDLKGKVNGAVETCGENFHRFDRYLNFSNPIIAKNFDPRACGWAYGMNVFDLDEWKKQNITDVYHSWQNLNHERQLWKLGTLPPGLITFWKRVYPLERSWHVLGLGYNPSVSQKDIERAAVIHYNGNLKPWLEIGIPKFRGYWSKFVDYDQVYLRDCNMSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C252</accession>
    <name>A0AAD5C252_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Histone H2A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_020685</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="8">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the histone H2A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009928">
        <property type="protein sequence ID" value="KAI7733702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C252"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00074">
        <property type="entry name" value="HFD_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000009">
        <property type="entry name" value="Histone H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002119">
        <property type="entry name" value="Histone_H2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032454">
        <property type="entry name" value="Histone_H2A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032458">
        <property type="entry name" value="Histone_H2A_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23430">
        <property type="entry name" value="HISTONE H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16211">
        <property type="entry name" value="Histone_H2A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00620">
        <property type="entry name" value="HISTONEH2A"/>
    </dbReference>
    <dbReference type="SMART" id="SM00414">
        <property type="entry name" value="H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00046">
        <property type="entry name" value="HISTONE_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 8" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="10">
        <location>
            <begin position="13"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="Histone H2A C-terminal" evidence="11">
        <location>
            <begin position="96"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF16211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7733702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="139" mass="14506" checksum="C4C7346F07EB50D3" modified="2024-05-29" version="1">MAGKSNTSVKKGGRGKAKAVSRSSKAGLQFPVGRIARFLKAGRYAQRVGAGAPVYLSAVLEYLAAEVLELAGNAARDNKKSRVGPRHLQLAVRNDEELSKLLGGVTIANGGVLPNIHATLLPKKAGKEKGDIGSASQEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C2G0</accession>
    <name>A0AAD5C2G0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_022159</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010140">
        <property type="protein sequence ID" value="KAI7732831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C2G0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7732831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="108" mass="12403" checksum="B8FD6FF2434703DF" modified="2024-05-29" version="1">METLQEVKAVKNVTSARNLILELQDKLKENIVTNMSHKEQSQDLRRKGWMLQMIDELLTKVPTSHPDKDNLQAKFCGLRVEANNIMKQWEIQCDNKLHESATSSPPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C408</accession>
    <name>A0AAD5C408_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Vps72/YL1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_004384</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009936">
        <property type="protein sequence ID" value="KAI7733596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C408"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029525">
        <property type="entry name" value="INO80C/Ies6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013272">
        <property type="entry name" value="Vps72/YL1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31200">
        <property type="entry name" value="INO80 COMPLEX SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31200:SF1">
        <property type="entry name" value="INO80 COMPLEX SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08265">
        <property type="entry name" value="YL1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00993">
        <property type="entry name" value="YL1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Vps72/YL1 C-terminal" evidence="5">
        <location>
            <begin position="73"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7733596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="123" mass="14569" checksum="E9E575A082FB57C6" modified="2024-05-29" version="1" fragment="single">MEQEVIESEMILPTYMPFKKIQMHDKYPKGQARGRHWKHLRQIIQAENYQNYPPHEPNYVNIESPPSMHPRKQICDITGFEAPYSDPRTHLRYANTEVFKLIRSLPNEYVQSYLGLRNAAVVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C4J0</accession>
    <name>A0AAD5C4J0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein GIGANTEA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_031923</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009888">
        <property type="protein sequence ID" value="KAI7733896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C4J0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042752">
        <property type="term" value="P:regulation of circadian rhythm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048586">
        <property type="term" value="P:regulation of long-day photoperiodism, flowering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026211">
        <property type="entry name" value="GIGANTEA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36319">
        <property type="entry name" value="PROTEIN GIGANTEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36319:SF1">
        <property type="entry name" value="PROTEIN GIGANTEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02081">
        <property type="entry name" value="GIGANTEA"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="151"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7733896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="338" mass="37046" checksum="56B30FF88690D4D0" modified="2024-05-29" version="1" fragment="single">MAATSDKWTDGLRFSSLFWPPPLDTQQRKAQITAYVDYFGQFTSEQFPEDIAELVRHQYPSQEKRLFDDVLATFVLHHPEHGHTVILPIISCIIDGTIEYDKNTPPFASFISLVCPNDDNEYSEQWALACGEILRILTHYNRPIFKVDQDRSVSGSQASTSKSPDGQTSSPAVQNEVKPLRPLSPWITDILLAAPLGIRSVVVSWGIMASSRGSGKHPQLIPSTPRWAVANGAGVILSVCDEEVARYETATLTAAAVPALLLPPPTTSMDEHLVAGLPALEPYARLFHRYYAIASPSATQRVLLGLLEAPPSWAPDALDAAVQLVELLRAAEDYASGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C4L4</accession>
    <name>A0AAD5C4L4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_026546</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009848">
        <property type="protein sequence ID" value="KAI7734029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C4L4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7734029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="59" mass="7023" checksum="8859FD59110FB0D5" modified="2024-05-29" version="1">MHINHQINFVHFNARIVLWADPVFNTLTPLLLKLISLFPLFIQKIDPFSQLMIFPFRSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C4S3</accession>
    <name>A0AAD5C4S3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_003981</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the major facilitator superfamily. Phosphate:H(+) symporter (TC 2.A.1.9) family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009625">
        <property type="protein sequence ID" value="KAI7734894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C4S3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051119">
        <property type="term" value="F:sugar transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17358">
        <property type="entry name" value="MFS_GLUT6_8_Class3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000218">
        <property type="entry name" value="facilitated trehalose transporter Tret1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044775">
        <property type="entry name" value="MFS_ERD6/Tret1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050549">
        <property type="entry name" value="MFS_Trehalose_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48021">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48021:SF23">
        <property type="entry name" value="SUGAR TRANSPORTER ERD6-LIKE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="49"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="88"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="120"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="145"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="179"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="203"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="284"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="324"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="351"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="385"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="12">
        <location>
            <begin position="55"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7734894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="416" mass="44679" checksum="A7C40C10C511DA60" modified="2024-05-29" version="1" fragment="single">MSFRDDNEEGRTASDGHLKKPFLHTGSWYRMGSRQSSVMTSSQTIKDRSVSVLACVLIVALGPIQFGFTGGYSSPTQAAITRDLKLSVSEFSLFGSLSNVGAMVGAIASGQIAEYIGRKGSLMIAAIPNIIGWLIISFAKDLSFLYLGRLLEGFGVGVVSYTVPVYIAEIAPQNMRGGLGSVNQLSVTIGIMLAYLLGLFVNWRMLAVLGTLPCSILIPALFFIPESPRWLAKMGMTDDFEASLQVLRGFDTDITVEVNEIKRAVASSNRRATIRFSDLKQRRYWFPLMIGIGLLVLQQISGINGVLFYASNIFRSAGISNSDAATLALGAIQVIATGISTWLVDKTGRRILLTVSSAGMTLSLAVVAASFFVKSFVKDSSSLYTAMGILSVVGVVGMVIGFSLGMGPIPWIIMSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C5M4</accession>
    <name>A0AAD5C5M4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GFO/IDH/MocA-like oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_004736</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Gfo/Idh/MocA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009474">
        <property type="protein sequence ID" value="KAI7735547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C5M4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006740">
        <property type="term" value="P:NADPH regeneration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055170">
        <property type="entry name" value="GFO_IDH_MocA-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42840:SF5">
        <property type="entry name" value="NAD(P)-BINDING ROSSMANN-FOLD SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42840">
        <property type="entry name" value="NAD(P)-BINDING ROSSMANN-FOLD SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22725">
        <property type="entry name" value="GFO_IDH_MocA_C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GFO/IDH/MocA-like oxidoreductase" evidence="2">
        <location>
            <begin position="62"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7735547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="160" mass="17654" checksum="6ABCCAA9923CEE17" modified="2024-05-29" version="1" fragment="single">VDMSLKLLKAGKHVLQAISEAETALSSYKSLHNNPSAQKIWAVAENYRFEPAFVESKKLVSEIGDMMSVQVLIEGSMNSSNPYFSSSWRRNFTGGFILDMGVHYIAGLRMLVGCEVAAVSATTSHVDTTLPPPDTISSLFDNVFFLAFLVNLRTDVQEFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C611</accession>
    <name>A0AAD5C611_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">H15 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_003904</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the histone H1/H5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009438">
        <property type="protein sequence ID" value="KAI7735757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C611"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031492">
        <property type="term" value="F:nucleosomal DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030261">
        <property type="term" value="P:chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045910">
        <property type="term" value="P:negative regulation of DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00073">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005819">
        <property type="entry name" value="H1/H5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005818">
        <property type="entry name" value="Histone_H1/H5_H15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11467">
        <property type="entry name" value="HISTONE H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11467:SF131">
        <property type="entry name" value="HISTONE H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00538">
        <property type="entry name" value="Linker_histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00624">
        <property type="entry name" value="HISTONEH5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00526">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51504">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="4 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="H15" evidence="8">
        <location>
            <begin position="53"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="122"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="24"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="37"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="130"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="159"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="186"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="250"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7735757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="270" mass="28122" checksum="CF06C838B3FAB722" modified="2024-05-29" version="1">MATEEAVVVTEATEPVTAPADVTPEEKVEAPVDVKPKKVKKASVKGKPRSPSLHPPYFEMIKEAIVTLKERTGSSQYAITKFIEEKQKNLPANFKKVLFTQLKKFVAAGKLVKVKASYKLPAASAPAKKPAAKPKTVAKPKTAKKKAPAKKAAVAKPKPAPKAKTAAKPKTVAKPKPAAKAKPVAKPKSVTKPAAKAKTVGKPKAVAAKAKPKAKAAAKPSKAARTSTRTSPGKKVAAAPKKAVVKKATVKSLKPKGGVAKKAAVKAGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C726</accession>
    <name>A0AAD5C726_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_007981</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009333">
        <property type="protein sequence ID" value="KAI7736200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C726"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="249"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="259"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7736200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="275" mass="31550" checksum="99BA8462A5EBCB3F" modified="2024-05-29" version="1">MNVRGLSIAHVKNDWMYHNLIHYCYTTVLSQRGTMPLQGRPHIYSNLYSRSSPFGHLKQANGGIDLARNLEVGNYYSRSHLLDSALRPTPSVQHFLSRHQQWLSNQSLTSSSSLRTEFGHANIRKDIMNQIQDRPSGLNKFHTFESRTQNGTMRPSQFLEDKKLWLPHDQRTDKWSPITRNCASSGSSDPFGVFSEKARNFVQSHSSFSYSRSLMNAQSEASFLHELRHEKGFNDKEKNHDLQLRLSQNKGTDEDKHPMSTSEINTALSLSLQET</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C914</accession>
    <name>A0AAD5C914_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_019529</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008995">
        <property type="protein sequence ID" value="KAI7737568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C914"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000003">
        <property type="entry name" value="Calmodulin A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039647">
        <property type="entry name" value="EF_hand_pair_protein_CML-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10891">
        <property type="entry name" value="EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13405">
        <property type="entry name" value="EF-hand_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="53"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7737568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="124" mass="14012" checksum="3F93A5249F53D699" modified="2024-05-29" version="1">MKFGRFENGRAKKALGMNSKIMMGRLGMFWDPDDQYESIGLDDMADLFVEDEPSLDEVKDAFSVFDKNGDGYIDAKELQNVLSEMGFLQISESDCRSMILGYSAHKDGKINFQAFLNVVEDAFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C9R8</accession>
    <name>A0AAD5C9R8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cation-transporting P-type ATPase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_025156</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIIA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008967">
        <property type="protein sequence ID" value="KAI7737722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C9R8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000045">
        <property type="entry name" value="ATPase 4 plasma membrane-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000191">
        <property type="entry name" value="Membrane H(+)-ATPase1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000211">
        <property type="entry name" value="Plasma membrane ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42861">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00120">
        <property type="entry name" value="HATPASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="66"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="101"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="355"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="420"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="494"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="527"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="12">
        <location>
            <begin position="18"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7737722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="659" mass="73029" checksum="D47B0E044B19DC53" modified="2024-05-29" version="1">MGGDTALSLEGIKNETVDLEKVPIEEVFEQLKCNREGLSSDEGAQRLEIFGPNKLEEKKESKILKFLGFMWNPLSWVMEAAAIMAIALANGGGKPPDWQDFVGIVCLLVINSTISFIEENNAGNAAAALMAGLAPKTKILTLCGCKEDLKKKVHAMIDKFAERGLRSLGVARQEVPQKSKDSAGGPWEFVGLLSLFDPPRHDSAETIRRALNLGVNVKMITGDQLAIAKETGRRLGMGTNMYPSSSLLGGHKDESIAGLPIDELIEKADGFAGVFPEHKYEIVKKLQERKHICGMTGDGVNDAPALKKADIGIAVADATDAARSASDIVLTEPGLSVIISAVLTSRAIFQRMKNYTIYAVSITIRIVFGFLFIALIWKFDFSPFMVLIIAILNDGTIMTISKDRVKPSPLPDSWKLKEIFATGVVLGGYLALMTVIFFWIMKDTDFFTEKFSVKSLRNSETEMMAALYLQVSIVSQALIFVTRSRSWSFVERPGFLLMGAFLAAQLVATLIAVYAEWGFARIKGVGWKWAGVIWLYSIVFYFPLDIMKFAIRYILSGKAWLSMIDNRTAFTTKKDYGRGEREAQWAHAQRTLHGLQAPDTSNLFNEKSSYRELSEIAEQAKRRAEVARLREVLTLKGHVESVVKLKGLDIDTIQQHYTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CAS8</accession>
    <name>A0AAD5CAS8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">phosphoenolpyruvate carboxylase</fullName>
            <ecNumber evidence="5">4.1.1.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_000215</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>oxaloacetate + phosphate = phosphoenolpyruvate + hydrogencarbonate</text>
            <dbReference type="Rhea" id="RHEA:28370"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="4.1.1.31"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PEPCase type 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.31" evidence="5"/>
    <dbReference type="EMBL" id="JAMZMK010008802">
        <property type="protein sequence ID" value="KAI7738511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008964">
        <property type="term" value="F:phosphoenolpyruvate carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015977">
        <property type="term" value="P:carbon fixation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048366">
        <property type="term" value="P:leaf development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1440.90:FF:000001">
        <property type="entry name" value="Phosphoenolpyruvate carboxylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.90">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021135">
        <property type="entry name" value="PEP_COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018129">
        <property type="entry name" value="PEP_COase_Lys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033129">
        <property type="entry name" value="PEPCASE_His_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30523">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30523:SF33">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE CARBOXYLASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00311">
        <property type="entry name" value="PEPcase"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00150">
        <property type="entry name" value="PEPCARBXLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00781">
        <property type="entry name" value="PEPCASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00393">
        <property type="entry name" value="PEPCASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0120">Carbon dioxide fixation</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" evidence="12">
        <location>
            <position position="172"/>
        </location>
    </feature>
    <feature type="active site" evidence="13">
        <location>
            <position position="578"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7738511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="869" mass="98875" checksum="C149CF388544950A" modified="2024-05-29" version="1">MGDRKTEQLASIDAQLRLLAPAKVSEDDKLIEYDALLLDKFLDVLQNVHGEALRETVQQCYELSAEYEGEHDPKKLDELGNVLTSLDAGDSIVIAKAFSNMLNLANLAEEVQIAHRRRIKIKTGDIGDEALATTESDIEETFKRLVNKLKKSPQEVFDALKNQTVDLVFTAHPTQAVRRSLLQKHGSYQFLIVIQAAFRTDEIRRSPPTPQDETRAGMSYFQETIWKGVPKFLRRVDTALKNIGINERVPYNAPLIQFSSWMGGDRDGNPRVTPEVTRDVCLLARMMAANMYFNQIEELMFELSMWRCSNELRVRAEEIYTSARRDVKHYIEFWKQVPFNEPYRVILGDVRDKLYNTRERSRHLLAHGFSDIPEESAYTYIEQFLEPLELCYRSLCACGDKVIADGTLLDFLRQVSTFGLALVRLDIRQESERHTDVLDAITQHLEIGSYRDWSEEKRQQWLLEELSGKRPLFGPDLPQTEEIADVLDTFHVLAELPSDCFGAYIISMATSPSDVLAVELLQRECHVKTPLRVVPLFEKLADLEAAPAAMSRLFSIDWYKNRIHGKQEVMIGYSDSGKDAGRLSAAWMLYQAQEKLVEVAKKYGVKLTMFHGRGGTVGRGATLEHGMNPPVSPRPEWRQLMDNLATYATEEYRSIVFKEPRFVEYFRLATPETEYGRMNIGSRPSKRKPSGGIESLRAIPWIFAWTQTRFHLPVWLGFGGSXXXDLVEMVFAKGDPGIAALNDKLLVSSDLLKFGESLRNNYVETKNLLLQIVGHKDLLEANPYLKQRLKLRDSYITTLNVCQAYTLKRIRDPNYHVTLRPHISKDGPEPSKPADELIKLNPASEYAPGLEDTLILTMKGIAAGMQNTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CC35</accession>
    <name>A0AAD5CC35_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_025155</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008967">
        <property type="protein sequence ID" value="KAI7737721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CC35"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37768">
        <property type="entry name" value="OS06G0694800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37768:SF2">
        <property type="entry name" value="OS06G0694800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="102"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="46"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7737721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="141" mass="14938" checksum="E5B95FDE39618D1F" modified="2024-05-29" version="1">MNRVNESNQLAKLALVTMAAGVLTLGSIDPAFAAKSGGRVGGQAFRSAPRSAPRSSSPRINNSRTNIYINPRVAPPLIGGYGYGYGGWGWSPFSFFAPGPSVAVGIGGGFDTLVLFMFLGAAAAVVRRIFRSRNDYDDEDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CCG5</accession>
    <name>A0AAD5CCG5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_002056</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008700">
        <property type="protein sequence ID" value="KAI7738893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CCG5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051222">
        <property type="entry name" value="PPR/CCM1_RNA-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47942:SF16">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT DOMAIN CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47942">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT (TPR)-LIKE SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12854">
        <property type="entry name" value="PPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="247"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="282"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="317"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="352"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="387"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="422"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="457"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="492"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="527"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="562"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="597"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="632"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="667"/>
            <end position="701"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7738893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="744" mass="84542" checksum="4C8F84878F42D9B2" modified="2024-05-29" version="1">MTLHVRSSKSIGKHSFHYIKVRLTFFSSNSFITQSPTFLNNNINNNNNTHHPINCPEFHFKNLLLSTVKTKSWKTNPNPFQQSLQELILDPQLVTKVLYSVKDHPRLALRFFHWVSSQPGCVLPDTAVVAVFDILAQRGLMNRAYPVMERVVCGDFDGVLERVLDCLVSGNLNANVADKVFDFMLFFLAKKTMVEQCLLVFDKMVRSKFLPDVKNCNRILKMLRGMRLVRRRNEVYSMMNEYGIKPNIITYNTLLDSYCKEGDIDQALHLLREMQTRGCAPNDVTYNALISGLVKEGDFEQAREMISKMTVLGLKVSSYSYNPLIRGYCEKGLIVKAFNLAEEMTVAGASPTVYTYNILMYGLCKQGRVLNARQQYSKMLKKNITPDIVSYNILIYGCSVFGSIAEAFSLLYELRRIDLFPTMITFNTLIQGLCTKGNMEKARQLKDEMVNLGFPPDVYTYTILVNGCYKVGDLTMAKEMFDEMITRGLKPDHYAYTTRIVGELTLGDTSTAFNLLEEMLEKGFMPNLITYNVFVHGLCKAANIEEANKLLKEMVDSGFVPDHVTYTSIICSYLDMGNIQKARDLFYEMETKGVNPTVVTYTVMIHAHAVTGRVESAFMYFMQMLKKGIMPNVITYNALINGYCKAGRMENAYECFSEMESRGLVANKYSYTILINGNCESGNWSEALRLYNEMVDRGIEPDSCTHSALLKQIGLNAKAHAVQYLENVVLGNYAHDEPKVVRYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CE34</accession>
    <name>A0AAD5CE34_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Chalcone synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_010728</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the thiolase-like superfamily. Chalcone/stilbene synthases family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008628">
        <property type="protein sequence ID" value="KAI7739385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CE34"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030639">
        <property type="term" value="P:polyketide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00831">
        <property type="entry name" value="CHS_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000014">
        <property type="entry name" value="Chalcone synthase 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000025">
        <property type="entry name" value="Chalcone synthase 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012328">
        <property type="entry name" value="Chalcone/stilbene_synt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001099">
        <property type="entry name" value="Chalcone/stilbene_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018088">
        <property type="entry name" value="Chalcone/stilbene_synthase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011141">
        <property type="entry name" value="Polyketide_synthase_type-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11877">
        <property type="entry name" value="HYDROXYMETHYLGLUTARYL-COA SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11877:SF108">
        <property type="entry name" value="POLYKETIDE SYNTHASE, TYPE III, THIOLASE-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02797">
        <property type="entry name" value="Chal_sti_synt_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00195">
        <property type="entry name" value="Chal_sti_synt_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00441">
        <property type="entry name" value="CHALCONE_SYNTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Chalcone/stilbene synthase N-terminal" evidence="5">
        <location>
            <begin position="2"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="Chalcone/stilbene synthase C-terminal" evidence="6">
        <location>
            <begin position="194"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="Chalcone/stilbene synthase N-terminal" evidence="5">
        <location>
            <begin position="352"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="domain" description="Chalcone/stilbene synthase C-terminal" evidence="6">
        <location>
            <begin position="585"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="748"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7739385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="748" mass="82325" checksum="D36C54366CF07249" modified="2024-05-29" version="1" fragment="single">ADYADYYFRVTKSEHMSNLKEKFKRDKTMIKKRFVFLTEEFLKENPNMCEYMSPSLNTRQDLLVTAIPKLGKEAAIKAIEEWGVSRSRITHLIFCTTSGIDMPGADLQLTKLLGLSPLGCFAGGTALRLAKDLAENNKGSRVLVVCSDSMASIFRGPSDNHIDSLVGQALFGDGAAAIIVGSDPDLSTEHPLFEIVSSNQTIVPDTEMAMSLNLREEGLTFHLHKDVPKMISENIESVLKHAVSPLGLSDWNSLFWIVHPGGRAILDNVELKLKLDKEKLRASRHVLSEYGNITSACVLFILNETRNKSLEDGKSTTGEGLDWGVLFGFGPGLTIETVVLRSQPVAKTVDVETIRQTQRAQGLATILAIGTATPFNCIHQADYADYYFRVTKSEHMTNLKEKFKRICDKTMIKKRFTFLTEEFLKENPNMCEYMSPSLNTRQDLLVTAVPNLGKEAAIKAIEEWGVSRSRITHLIFCTTSGIDMPGADLQLTKLLGLSPLVNRLMIYQQGCFAGGTALRLAKDLAENNKGSRVLVVCSDSMASIFRGPSENHIDSLVGQALFGDGAAAIIVGSDPDISTEHPLFEIVSANQTIVPDTEMAMNLHLREEGLTFHLHKDVPKMISENIESVLKHAVSPLGLSDWNSLFWIVHPGGRAILDNVELKLKLDKEKLRASRHVLSEYGNLNSACILFILNEMRNKSLEDGKSTSGEGLDWGVLFGFGPGLTIETVVLRSQPAATPAATITDGAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CE37</accession>
    <name>A0AAD5CE37_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Polygalacturonase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_009431</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 28 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008454">
        <property type="protein sequence ID" value="KAI7740358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CE37"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004650">
        <property type="term" value="F:polygalacturonase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051801">
        <property type="entry name" value="GH28_Enzymes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000743">
        <property type="entry name" value="Glyco_hydro_28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31339:SF44">
        <property type="entry name" value="PECTIN LYASE-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31339">
        <property type="entry name" value="PECTIN LYASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00295">
        <property type="entry name" value="Glyco_hydro_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00710">
        <property type="entry name" value="PbH1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7740358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="480" mass="53831" checksum="5F9C6184FF9C0617" modified="2024-05-29" version="1">MVETLINLRRSISSYKTLFALLWIVVFTSLFIWQRSFLDGFLIFRRPIVSVRPLPLFRPVAYNITEFGGVGDGVTLNTVAFERAVFAISKLGKKGGGQLNVPAGKWLTAPFNLTSHFTLFLDQDAVILGVDDEKLWPLMPPLPSYGYGREHPGPRYGSLIHGQNLRDVVITGNNGTIDGQGQRWWKRYRQKLLNNTRGPLVQIMYSSDIVISNITLRNPPFWTLHPYDCKNVTIRNMTILAPLFEAPNTDGIDPDSCEDMVIEDSYISVGDDAIAIKSGWDQYGTAYGRPSKNILIRNLIVRSMVSAGISIGSEMSGGVSNVTVENVLIWNSRRAVRIKTAAGRGAYIRDIKYRNITFENVRVGIIIKTDYNEHPDDGFDRKAFPVIRDISYISIHGEGVRVPVRIHGSSDIPVRNVTFRDMSVGITYKKKHIFQCAYVSGKVIGSIFPKPCENLDLYDEQGTLVKKSVSENASDIDYDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CFW3</accession>
    <name>A0AAD5CFW3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_033045</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008323">
        <property type="protein sequence ID" value="KAI7740918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CFW3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34555:SF7">
        <property type="entry name" value="DUF3741 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34555">
        <property type="entry name" value="INTEGRAL MEMBRANE HEMOLYSIN-III-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7740918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="243" mass="27319" checksum="39B7F5ADBFD01144" modified="2024-05-29" version="1">MINSEININDVGTNVAGTAAFHKQTVFPVKKVALREVQNSNNNLTRDTYSASLLPADGQLFVDASKTCGTKRATPDYSAPSNLTNHGAQERFNFLRNKHEGYQGKNVGYPQSASLPQIKQEVSQINGKNMHYGSFVAPNQMASKTSVSRLNEFNDERRTDRLVNLQNFIDQCDKSDYRDNIQSLLHLSPLELSRHAVELEKRAIHLTIEEGKEMQRMQALNILGKPSITRSSMPITQHIQHKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CI10</accession>
    <name>A0AAD5CI10_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_008896</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Secondary metabolite biosynthesis; terpenoid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008107">
        <property type="protein sequence ID" value="KAI7741914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CI10"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016712">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051762">
        <property type="term" value="P:sesquiterpene biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11072">
        <property type="entry name" value="CYP71-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000011">
        <property type="entry name" value="Cytochrome P450 83B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47955:SF16">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47955">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 71 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8 9" id="KW-0349">Heme</keyword>
    <keyword evidence="6 8 9" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="8">
        <location>
            <position position="444"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7741914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="502" mass="57593" checksum="F7075EB285DE7D6E" modified="2024-05-29" version="1">MEIFSSFETFLLSLFSCIILVIFILIKWFSFDSKNRKNIPPSPPKLPIIGNFHQLGSSTNRNLQILSQKYGPLMLLHLGSIPTLVASSNEAAREIMKTHDVSFCSRPSLIASNIFFYGSKDVAFAPYGEYWRQLKSLVVLKLLSNIRVKSYKKVRENEIVDMIQVLGESCGTTIDMRPMFTSLTNNIICRIALGRKLDGPKYQKLLSRIADMFNAFYIGDYFPWLSWVDHIVGSIQRAEKIAKECDEFLERTIEEHVNNKKREDAKGDEEEDFVDILLDLQKDNTSGLQLQRDTLKALILEVFAAGTDTTQTSLEWALSELIRNPRVMKKLQQEITEIGKGKSMIYEEDLERMPYIKAVVKESLRLHTPLPFLLPRKLVQDVKVMGYDIAADTQVIINAWAIARDPTLWEEPNEFRPERFLNNSIKYQGLNFEWIPFGAGRRSCPGIQFSLPVMELALANIVYKFDMALPNEIKNEELDMTEAFGITTYRKFPLVVSVSPRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CIB6</accession>
    <name>A0AAD5CIB6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_026180</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008005">
        <property type="protein sequence ID" value="KAI7742381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CIB6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052101">
        <property type="entry name" value="Plant_StressResp_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47983:SF3">
        <property type="entry name" value="OS05G0135800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47983">
        <property type="entry name" value="PTO-INTERACTING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="137"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7742381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="396" mass="43832" checksum="D7DE48454B1633A4" modified="2024-05-29" version="1" fragment="single">MAGLKESDHLTLPFKEIEVATRNFETCIGKGGYGRVYRGELLIAGTRTMLSQLGYTSIEEVPSDKQPTVIQPITVPAHQVAVFSAIEAKPTDKQLVHYFKRVQILLSFATIKNRPKSNFAAVPALQMGELKKITGNFHVSSKIGEGLFGSVYHGVLRSGQAAAIKMLNSSEQPDQDFLAQVSMASRLKHDNVIQMLGYCVDDGSKGHKGAQQTRPVLSWAERVKIAFGAAKGLVYLHETAHIVHRDIKSSNVLLFDDHVAKIADFDLSNQDPNMSGRTQSTRTFNFGSHAPDFGVVLLELLTGRKPVDRTLPRGQQSLVTWATSKFDEDKVKECIDTRLNGEYPRKEVAKMAAIATLCLQYEPEHRPDMRMVVKEMQLLMDPSSRPLAGASEVSRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CL28</accession>
    <name>A0AAD5CL28_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_029898</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007635">
        <property type="protein sequence ID" value="KAI7743896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CL28"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030126">
        <property type="term" value="C:COPI vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050844">
        <property type="entry name" value="Coatomer_complex_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19876">
        <property type="entry name" value="COATOMER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19876:SF38">
        <property type="entry name" value="COATOMER SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="47"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="89"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="131"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7743896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="159" mass="18610" checksum="B2C0E0DF4FEA874B" modified="2024-05-29" version="1" fragment="single">MLTKFETKSNRVKGLSFHSKRPWILASLHSGVIQLWDYRMGTLIDRFDEHDGPVRGVHFHHSQPLFVSGGDDYKIKVWNYKLHRCLFTLLGHLDYIRTVQFHHENPWIVSASDDQTIRIWNWQSRTCISVLTGHNHYVMCALFHPKEDLVVSASLDQTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CLA8</accession>
    <name>A0AAD5CLA8_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_011934</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007955">
        <property type="protein sequence ID" value="KAI7742596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CLA8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042051612" evidence="2">
        <location>
            <begin position="24"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7742596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="126" mass="13958" checksum="B7ADEEF033DA9F34" modified="2024-05-29" version="1" fragment="single">KKKKKRTAFFIGLPICLHDLFLGITSSSIKEHPSSSTIANINFTPYHPKSSSTTKRPNPSPPNKTTTVADPSQLLSDPPRHYHCNCSKSLPPAARRLPQSSLLSYAAIAAHRYMYEISAVNQEKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CNE1</accession>
    <name>A0AAD5CNE1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Autophagy-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_032026</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the ATG8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007631">
        <property type="protein sequence ID" value="KAI7743915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CNE1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16128">
        <property type="entry name" value="Ubl_ATG8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004241">
        <property type="entry name" value="Atg8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10969">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEINS 1A/1B LIGHT CHAIN 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02991">
        <property type="entry name" value="ATG8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0072">Autophagy</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7743915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="175" mass="19822" checksum="67E3FBC15927B5ED" modified="2024-05-29" version="1" fragment="single">RRQAEAARIREKYPDRIPVIVEKAERSDVPDIDKKKYLVPADLTVGQFVYVVRKRIKLSAEKAIFIFVKNVLPPTAAMMSAIYEENKDEDGFLYMTYSAAFEVGQPSNQSVPTTVTGWEWLPFEENRHFHFGAAIVHHSNQMLVSVALTTKAGNLRLMLGMGRVGLYKELADICC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CNL9</accession>
    <name>A0AAD5CNL9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_004826</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007680">
        <property type="protein sequence ID" value="KAI7743781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CNL9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05121">
        <property type="entry name" value="ABC1_ADCK3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004147">
        <property type="entry name" value="ABC1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051130">
        <property type="entry name" value="Mito_struct-func_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43173">
        <property type="entry name" value="ABC1 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43173:SF12">
        <property type="entry name" value="PROTEIN KINASE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03109">
        <property type="entry name" value="ABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="132"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7743781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="479" mass="54322" checksum="7C73A31C90861180" modified="2024-05-29" version="1">MPLDFKDIQEKISVGFKPWHRSFQFWVRALDIYTDYKVFQVRVNFEKDEKKAEAMWESQHEHAADKVYSMCSDMGGFFLKLAQILGKPDLAPAAWVRRLVTLCDQAPATPSNMVQLVIEEELCKNVDEMFERFEWSPIGSASIAQVHRARLKGDKNDVVVKVQHPGVQDLMMTDLRNMKAFALFIQKTDVKFDFFSVCKEMEKQIGYEFDFTREADAMDRIRTFLLKVNRKAPVKVPRVIPNAVTRRVLVMEYIDGVPILNLGDEIKKRGINPDGKMAAAAKQNILKSLTLAYGQMILKSGFFHADPHPGNILICNGSGVALLDYGQVKDLPNALRLGYANLVLAIADNDHARASEGFRELGIDTLSLCSNEQAELFKLAQVMFDTKLPPGVKVLQPFSDDSSIKKVAVQAFPEELFSVLRTVQLLRGLSVGLGINYSCAEQWRPIAEEALYHAGRLKVEDMKTREGSKHGFIRRIFWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CQA5</accession>
    <name>A0AAD5CQA5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">25S rRNA (uridine-N(3))-methyltransferase BMT5-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_032871</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007016">
        <property type="protein sequence ID" value="KAI7746221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CQA5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070042">
        <property type="term" value="F:rRNA (uridine-N3-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019446">
        <property type="entry name" value="BMT5-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10354">
        <property type="entry name" value="BMT5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="25S rRNA (uridine-N(3))-methyltransferase BMT5-like" evidence="2">
        <location>
            <begin position="70"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7746221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="149" mass="17260" checksum="50DAC871B6633CC5" modified="2024-05-29" version="1">MYCSDRPAGYLKSPQPLVDEDKKKLEKENEEPLETKEPEEDMKKDEEGQRKTEIKDLYNTDQKILIGCVGDFSRARYIASELGDASKMIVMSQYSCVDELETMYENARSQMLDLTKLGARIVFDVDFKKFAKDSRINHTQYDIILLDLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CQF6</accession>
    <name>A0AAD5CQF6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DRBM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_024652</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007046">
        <property type="protein sequence ID" value="KAI7746087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CQF6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014720">
        <property type="entry name" value="dsRBD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46031">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46031:SF16">
        <property type="entry name" value="DOUBLE-STRANDED RNA-BINDING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00035">
        <property type="entry name" value="dsrm"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00358">
        <property type="entry name" value="DSRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50137">
        <property type="entry name" value="DS_RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DRBM" evidence="5">
        <location>
            <begin position="4"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM" evidence="5">
        <location>
            <begin position="82"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="256"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="257"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7746087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="382" mass="41241" checksum="E1FFC0051AFD84BC" modified="2024-05-29" version="1">MAHLHKTKLQTFAQKKKIPLPTYTVETQGPPHARLFKAQVTIDGNTYAGPGFSSTLKDAEHEAAKVALVSLSQDGAQEDDTLYKSLLQELAQKKGLILPVYTTNTTGQPHMPCFVSTVEVSGQSYVGEDARTKKQSEMNAAKVAYKALTEGGPQAKNATAISNTVGASSSGSCSLQTVMTHKNELTSSKVHTIINSGPTYTIPDDPEKAKGYLELKDLQDVIEEHKMTLSPVMIQPQPQSTPNVVPFAKPDIKQKNDVTQASQGSGSNVKRVEPSFNERTLTPVMDLDSAKLSPSVVDVPKPSMKLTNVESQASEGSVSNGKKIQPFVKEANLNIVMDSGSGMPHSIKIVIRPHVQGMTYDGPIQVADEEWVAMKVKVEDGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CQR6</accession>
    <name>A0AAD5CQR6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">tyrosine--tRNA ligase</fullName>
            <ecNumber evidence="4">6.1.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Tyrosyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_006998</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>tRNA(Tyr) + L-tyrosine + ATP = L-tyrosyl-tRNA(Tyr) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10220"/>
            <dbReference type="Rhea" id="RHEA-COMP:9706"/>
            <dbReference type="Rhea" id="RHEA-COMP:9707"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78536"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.1" evidence="4"/>
    <dbReference type="EMBL" id="JAMZMK010007436">
        <property type="protein sequence ID" value="KAI7744801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CQR6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004831">
        <property type="term" value="F:tyrosine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006437">
        <property type="term" value="P:tyrosyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000085">
        <property type="entry name" value="Tyrosine--tRNA ligase 1 cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000103">
        <property type="entry name" value="tyrosine--tRNA ligase 1, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002305">
        <property type="entry name" value="aa-tRNA-synth_Ic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023617">
        <property type="entry name" value="Tyr-tRNA-ligase_arc/euk-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050489">
        <property type="entry name" value="Tyr-tRNA_synthase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006330">
        <property type="entry name" value="PRK08560.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46264:SF4">
        <property type="entry name" value="TYROSINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46264">
        <property type="entry name" value="TYROSINE-TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00579">
        <property type="entry name" value="tRNA-synt_1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006588">
        <property type="entry name" value="TyrRS_arch_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="8 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 13" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 13" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00033323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7744801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="355" mass="40696" checksum="D4F0AF5BD2D794AE" modified="2024-05-29" version="1">MMSVEEKFRIVRSVGEECIQEEELLNLLTKKPQPICYDGFEPSGRMHIAQGIMKTLNVNKLTSAGCKVKIWIADWFALLNDKMGGDLDKIQTVGRYLIEIWKAAGMNLEGGQVEFLWSSEEINSRAHEYWPIVMEIARRNKLPRIVRCGQAMGRDERHELTAAQIFYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIKRKNKPIILSHHMLPGLKKGQEKMAKSDPSSAIFMEDEEDDVISKINNAYCPPNVVDKNPCLEYIKYIVLPWFSEFKVERKAESGGEKIFTSFEELAADYEKGDLHPADLKPALSKALNRILQPVREHFKHDENAKALFMKVKGFTATKRSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CQU4</accession>
    <name>A0AAD5CQU4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_031835</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007044">
        <property type="protein sequence ID" value="KAI7746114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CQU4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000502">
        <property type="term" value="C:proteasome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009553">
        <property type="term" value="P:embryo sac development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009555">
        <property type="term" value="P:pollen development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010498">
        <property type="term" value="P:proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000009">
        <property type="entry name" value="26S protease regulatory subunit 6A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000076">
        <property type="entry name" value="26S protease regulatory subunit 6A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000037">
        <property type="entry name" value="26S protease regulatory subunit 6A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050221">
        <property type="entry name" value="26S_Proteasome_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032501">
        <property type="entry name" value="Prot_ATP_ID_OB_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23073">
        <property type="entry name" value="26S PROTEASOME REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16450">
        <property type="entry name" value="Prot_ATP_ID_OB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0647">Proteasome</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="12">
        <location>
            <begin position="185"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="28"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7746114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="405" mass="45544" checksum="118FD0172C6D83E0" modified="2024-05-29" version="1">MATPMVEDTSFEDDQLSSMSTEDIQRASRLLDNEIRILKEELQRTNLELDSFKEKIKENQEKIKLNKQLPYLVGNIVEILEMNPEDEAEEDGANIDLDSQRKGKCVVLKTSTRQTIFLPVVGLVDPDKLKPGDLVGVNKDSYLILDTLPSEYDSRVKAMEIQELVEAIVLPMTHKERFQKLGIRPPKGVLLYGPPGTGKTLMARACAAQTNATFLKLAGPQLVQMFIGDGAKLVRDAFQLAKEKSPCIIFIDEIDAIGTKRFDSEVSGDREVQRTMLELLNQLDGFSSDERIKVIAATNRADILDPALMRSGRLDRKIEFPHPTEEARARIMQIHSRKMNVHPDVNFEELARSTDDFNGAQLKAVCVEAGMLALRRDATEVNHEDFNEGIIQVQAKKKSSLNYYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CRL2</accession>
    <name>A0AAD5CRL2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein PLASTID REDOX INSENSITIVE 2, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_011756</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006861">
        <property type="protein sequence ID" value="KAI7747001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CRL2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039349">
        <property type="entry name" value="PRIN2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35987">
        <property type="entry name" value="PROTEIN PLASTID REDOX INSENSITIVE 2, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35987:SF3">
        <property type="entry name" value="PROTEIN PLASTID REDOX INSENSITIVE 2-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7747001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="169" mass="19199" checksum="8D27F959AF7D4270" modified="2024-05-29" version="1">MAVFHTPIVSLSSTGLITSLVPTTSKTLILANVRSSSYNIKHFPSKTSHLFHLRAAPLEKYVYPDPIPEFALHETKKFREELKKKLYSERDMFGDDLDTVVDTCTELFSHFLHNEYGGPGTLLVEPFTNMLIELKQKKLPGANLAARASLLWAQNYLDCDWDIWNSKSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CSS0</accession>
    <name>A0AAD5CSS0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Cytochrome b561 and DOMON domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_032539</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006713">
        <property type="protein sequence ID" value="KAI7747646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CSS0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd08760">
        <property type="entry name" value="Cyt_b561_FRRS1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09631">
        <property type="entry name" value="DOMON_DOH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006593">
        <property type="entry name" value="Cyt_b561/ferric_Rdtase_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045266">
        <property type="entry name" value="DOH_DOMON"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005018">
        <property type="entry name" value="DOMON_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23130">
        <property type="entry name" value="CYTOCHROME B561 AND DOMON DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23130:SF115">
        <property type="entry name" value="OS01G0680900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03188">
        <property type="entry name" value="Cytochrom_B561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03351">
        <property type="entry name" value="DOMON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00665">
        <property type="entry name" value="B561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00664">
        <property type="entry name" value="DoH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50939">
        <property type="entry name" value="CYTOCHROME_B561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50836">
        <property type="entry name" value="DOMON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042043447" description="Cytochrome b561 and DOMON domain-containing protein" evidence="9">
        <location>
            <begin position="25"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="213"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="241"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="279"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="319"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="343"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="DOMON" evidence="10">
        <location>
            <begin position="52"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b561" evidence="11">
        <location>
            <begin position="168"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7747646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="382" mass="42486" checksum="EF4E5C728E076C54" modified="2024-05-29" version="1">MISGSRFGFLAVFVSFVAVSVVLGDQCNEDINSILPLPYANMSHMVCKPIWNSYVIRFSQSKDNVVTIVLSTTYTSGYVAMGFSRDGMMLNSSAMVGWITRKGTPVIKQYYAEGFSPSQVKHDKGELPLTGIPPFVTVYGGNIHLAYQLKYPSRLKTQPILLAFSSKYPEHHLLSVHDDKTTIHLDFSQGSSGSKSNSVTPSNNLHRSKTTHGALGLLGWGIILPYGAIVARYFKHKEPLWYYLHVGFQFVGFIFAVAAGFVGLNMYNKFNFHAPAHKGLGITAIVLSVLQVMAFFIRPEKDSKHRRYWNWYHHYVGRIALFFGCLNILLGIQLADAGSSWKIGYGFVIASVILTSIVLEVFKLKAPNKFPPNPSLPLDPAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CTN5</accession>
    <name>A0AAD5CTN5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_030596</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006994">
        <property type="protein sequence ID" value="KAI7746345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CTN5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044661">
        <property type="entry name" value="MED15a/b/c-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33137">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 15A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33137:SF4">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 15A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7746345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="173" mass="19991" checksum="C5FB7C4C91B6E389" modified="2024-05-29" version="1">MMLTIETRLQNPVRSSAAALYNSVNPSVPLASSANNGDWQDEVYQQIKAMKDLYDRYLLDSTAHTGNLNGEDVLEEVYQKIKAMKDLYLLDLIDVHQKVASKLKQHHSYPRQLHEKLKLFKEMVERFIQFLQFPKHGKLANYKDKLGTYERQIISVINTTKRKPGSSQQQPQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CUY2</accession>
    <name>A0AAD5CUY2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Aminotransferase class I/classII large domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_007615</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006526">
        <property type="protein sequence ID" value="KAI7748457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CUY2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016212">
        <property type="term" value="F:kynurenine-oxoglutarate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000096">
        <property type="entry name" value="ATP-binding cassette sub-family A member 3-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000086">
        <property type="entry name" value="Kynurenine--oxoglutarate transaminase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051326">
        <property type="entry name" value="Kynurenine-oxoglutarate_AT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43807:SF12">
        <property type="entry name" value="AMINOTRANSFERASE, CLASSES I AND II FAMILY PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43807">
        <property type="entry name" value="FI04487P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="6">
        <location>
            <begin position="29"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7748457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="393" mass="43422" checksum="AE6BB9DE38AF0B92" modified="2024-05-29" version="1">MEKKLSSIAKSLKPSPIQQLSLLAQRGNAVNLAEGFPDFPAPPHIKAAALSAITSDFNQYRHVQGICSLLVDKMKQMHGLDVDPLTDIAICCGQTEAFAATMFAIINPGDEVILFEPSFETYGTCISMAGGIPVYVALDPPYWTLDEVKLLDAFTAKTKALVLNSPHNPTGKVFNMGELEIIAEYCRTKDCIAITDEVYEHIIYDNEPHISLASLPGMQNRTIITSSLSKTYSVTGWRVGWAIGPSCIASAIRNIHTKVTDSAPAPFQEAALTALRSPPEYYISLRAEYESKRDFVFKLLCQVGFQVEFKPLGSFFIFAALPETCKLTDVEFVEELIKEAGVVAVPGCGFFHTKHDYHNRYIRFAFCKDDATLTSAARKMQQLVDASGRLRFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CW26</accession>
    <name>A0AAD5CW26_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_023646</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006388">
        <property type="protein sequence ID" value="KAI7749311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CW26"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052583">
        <property type="entry name" value="ATP-helicase/E3_Ub-Ligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45865:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SHPRH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45865">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SHPRH FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7749311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="302" mass="34655" checksum="C1BF72A3B8A5A904" modified="2024-05-29" version="1" fragment="single">MGRRKQIRPRRAGGGDSGSVSKEESCDPNASEALENESSGDEKPFYIEVDRSNWGSEEHYDIAEVVLINLNVCEEFHVQKSEEQCSWDDDKYKLRFRLRNVNDLIARSRMKVKQKLGHWHWANISASDIYLEFIEKRESLDTDAHVMVTGNFDEPSEGVSGLVHLVDMRYLTLRPVGSTFSGSLPSIKLRVEIQKYTFEACESLLEDARRSPWQKSMMNVMAWLRPEVMTSEARYGYKMPADMAVGLEPDEESVVSRKQARFDVSGFYEAIKPSKDSPMLTVDGLPDLLPELRPYQRRAAFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CW29</accession>
    <name>A0AAD5CW29_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ribosomal protein L2 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_002331</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006763">
        <property type="protein sequence ID" value="KAI7747410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CW29"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000020">
        <property type="entry name" value="60S ribosomal protein L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.950.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000006">
        <property type="entry name" value="60S ribosomal protein L8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.950.10">
        <property type="entry name" value="Ribosomal protein L2, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01320_A">
        <property type="entry name" value="Ribosomal_uL2_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002171">
        <property type="entry name" value="Ribosomal_uL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023672">
        <property type="entry name" value="Ribosomal_uL2_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022669">
        <property type="entry name" value="Ribosomal_uL2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022671">
        <property type="entry name" value="Ribosomal_uL2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014726">
        <property type="entry name" value="Ribosomal_uL2_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022666">
        <property type="entry name" value="Ribosomal_uL2_RNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007180">
        <property type="entry name" value="PRK09612.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13691:SF16">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13691">
        <property type="entry name" value="RIBOSOMAL PROTEIN L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03947">
        <property type="entry name" value="Ribosomal_L2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00181">
        <property type="entry name" value="Ribosomal_L2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002158">
        <property type="entry name" value="Ribosomal_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01383">
        <property type="entry name" value="Ribosomal_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01382">
        <property type="entry name" value="Ribosomal_L2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00467">
        <property type="entry name" value="RIBOSOMAL_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL2 RNA-binding" evidence="6">
        <location>
            <begin position="11"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL2 C-terminal" evidence="5">
        <location>
            <begin position="96"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="210"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7747410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="259" mass="28060" checksum="0A6497F50C6BD8E6" modified="2024-05-29" version="1">MGRVIRAQRKGAGSVFKSHTHHRKGPARFRSLDFGERNGYLKGVITEIIHDPGRGAPLARVTFRHPFRYKHQKELFVAAEGMYTGQFVFCGKKANLMVGNVLPLRSIPEGAVVCNVEHHVGDRGSFARASGDYAIVISHNPDNGTTRVKLPSGAKKIVPSGCRAMIGQVAGGGRTEKPMLKAGNAYHKFRVKRNCWPKVRGVAMNPVEHPHGGGNHQHIGHASTVRRDAPPGQKVGLIAARRTGRLRGQAAATAAKADK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CWS8</accession>
    <name>A0AAD5CWS8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">AP-3 complex subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_012156</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Subunit of non-clathrin- and clathrin-associated adaptor protein complex 3 (AP-3) that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. AP-3 appears to be involved in the sorting of a subset of transmembrane proteins targeted to lysosomes and lysosome-related organelles. In concert with the BLOC-1 complex, AP-3 is required to target cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Clathrin-coated vesicle membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the adaptor complexes large subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006277">
        <property type="protein sequence ID" value="KAI7749798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CWS8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030123">
        <property type="term" value="C:AP-3 adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030665">
        <property type="term" value="C:clathrin-coated vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026740">
        <property type="entry name" value="AP3_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056314">
        <property type="entry name" value="AP3B1/2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029390">
        <property type="entry name" value="AP3B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026739">
        <property type="entry name" value="AP_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002553">
        <property type="entry name" value="Clathrin/coatomer_adapt-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11134">
        <property type="entry name" value="ADAPTOR COMPLEX SUBUNIT BETA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01602">
        <property type="entry name" value="Adaptin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24080">
        <property type="entry name" value="AP3B1_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037096">
        <property type="entry name" value="AP3_complex_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01355">
        <property type="entry name" value="AP3B1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6 11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AP-3 complex subunit beta C-terminal" evidence="13">
        <location>
            <begin position="776"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="706"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="716"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="733"/>
            <end position="754"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR037096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7749798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="1064" mass="117568" checksum="776613E95EF7FE6A" modified="2024-05-29" version="1">MFTQLSTTADNFSKASSMVFRIGTDAHLYDDPDDVSISPLLDSKFDSEKCEALKRLLALIAQGLDVSNYFPQVVKNVASQSLEVKKLVYLYLLHYAERQRDLGDQNPLVRAWALRTMAGIRLHVISPLVLVAVRKCARDPSVYVRKCAANALPKLHDLRLEENAAAIIEIIEILLNDNSPGVVGAAAAAFAFVCPNDLPVIGRNYKRLCEILPDVEEWGQIILIGILLRYVIARHGLAKESIMASLLPNIFSNEKTGSESDTGYLEIKKTYEIGLQEPELVAMVSRSYLAGPDKHLLHLNNSDVISSDYISSRFTSSKTDDNVKILLQCTLPLLWSRNSAVVLAAAGVHWIMAPMEEISKIVKPLLFLLRSSDASKYVVLCNIQVFAKVMPSLFAANIEDFFISSSDAYQVKALKLEILSSIATDASISVIFQEFQDYVRDPDRRFAADTVAAIGVCAKRNPQVANTCVEGLLALISPKSINSTSGSMDDEAVVLIQALTSIKDIITQDPLSHENAIIYLIRNLDAIKVPVARAMIVWIVGEYNSIGNIIPKMVSIILRFLARGFPSESIETKYQILNAAVKVLLNSKEEDFDTAKSILNYVFELAKFDFNYDVRDRARVLRKLLSCYFSSSGLEEKPDNNEIWLLLAEHIFGEKVKPSTEMVNNRFYLPGSLSQIVFHAAPGYEPLPEPCSLTESDMVKGLEEPAQAEANDSDVDSGSLNEEGSYYSEDSVTTSTRTDSESAASESDNNNTDSLINLSDVGKPQEETNDLGELMSKGDLESWLDDNDNPGPSQNVAERVPSARISIGDIKESAVSPPLVSVDTFFENCSSDSMSKIFLTNEDSESLDQGSSLESQKDVPVFAPMEEISILEPGQTIKRVIHVHFHHHLLPLKLILWCNDKKHPVKLRPDIGFFLKPLMMDLDAFSQKESQLPGMFEYTRRCSFTDHLAELGSDGKDGDVSPTKDSFLIICQCLASRMLSNANLHLVSVNMPVDANLNDASGLCLRFSGEILSNSIPCLVSVTLDGKCFEPLNASVKINCEETVFGLNLLNRIVNFLAEPQLTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CYC3</accession>
    <name>A0AAD5CYC3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Methionine aminopeptidase</fullName>
            <ecNumber evidence="7">3.4.11.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_008920</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.</text>
            <dbReference type="EC" id="3.4.11.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <cofactor evidence="6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <cofactor evidence="6">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="6">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="6">Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe(2+)-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase M24A family. Methionine aminopeptidase type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.18" evidence="7"/>
    <dbReference type="EMBL" id="JAMZMK010006306">
        <property type="protein sequence ID" value="KAI7749700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CYC3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004239">
        <property type="term" value="F:initiator methionyl aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01086">
        <property type="entry name" value="MetAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.230.10:FF:000011">
        <property type="entry name" value="Methionine aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01974">
        <property type="entry name" value="MetAP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001714">
        <property type="entry name" value="Pept_M24_MAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002467">
        <property type="entry name" value="Pept_M24A_MAP1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00500">
        <property type="entry name" value="met_pdase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43330">
        <property type="entry name" value="METHIONINE AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43330:SF1">
        <property type="entry name" value="METHIONINE AMINOPEPTIDASE 1B, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00599">
        <property type="entry name" value="MAPEPTIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00680">
        <property type="entry name" value="MAP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6 7" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 6 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 6 7" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Peptidase M24" evidence="8">
        <location>
            <begin position="64"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="220"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="283"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="283"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7749700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="299" mass="33286" checksum="F4113016412C90F3" modified="2024-05-29" version="1">MRIRREREQNTSTPTKRRIPLRRLKVSPRLPVPDHIQKPPYVGSKLLPEISSEFQIHDEEGITRMRAACQLAARVLEYAGTLVRPSVTTNEIDKAVHHMIIDNGAYPSPLGYGGFPKSVCTSVNECMCHGIPDSRQLQDGDIINIDVTVYLNGYHGDTSKTFFCGNVNDAAKRLVKVTEECMERGIAVCKDGALFRKIGKRISEHAEKFGFGVVERFVGHGVGTVFHSEPLIMHHRNEKPGSMVKGQTFTIEPILTLGTTECITWEDNWTTLTKDGSLAAQFEHTILITATGAEILTKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CYM9</accession>
    <name>A0AAD5CYM9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_022779</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006464">
        <property type="protein sequence ID" value="KAI7748775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CYM9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7748775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="98" mass="11164" checksum="A10F548EFF286268" modified="2024-05-29" version="1" fragment="single">SKLASPLKTHRLLHTSVIHIPLLLGFSDHRRPPLHGGPPITSPETTTTTTCFSSSLQLLENVGVKNVKLLYQRVQHQDEVIKIKEDPHKFMKGYLFLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CZB2</accession>
    <name>A0AAD5CZB2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sulfotransferase</fullName>
            <ecNumber evidence="3">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_022496</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the sulfotransferase 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010006121">
        <property type="protein sequence ID" value="KAI7750587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CZB2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11783">
        <property type="entry name" value="SULFOTRANSFERASE SULT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sulfotransferase" evidence="4">
        <location>
            <begin position="62"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7750587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="323" mass="37978" checksum="73D99C917B9D3754" modified="2024-05-29" version="1">MSSAPDDMEYNKICEKHKHLIETLPKGNGWRVQHLYNYNGFWLPPMIIKNNLLIHTYFKSQPTDIYLASFMKSGNTWLKALMFSTLNRHRYTFSNHFLHKHNPHSAFPFLEFLSYPVTGFTVPRLFATHSARTLLPDCMSSGRFVYVCRDPKDVLVSKWLFMSRLRAKDLPPLSLEDAFELFCRGVSEYGPYWEHVMSYWRANLEYPDKILFVKYEEVKKHPEVVVRKLAAFMGKPITEEEEEKGVVREIVKLCSFENLSNLDVNKNGVEKFGDMVEVENHTYFRKGEIGDWKNYLSEEMKERVDRITYEKLRGSGLILTTMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CZT5</accession>
    <name>A0AAD5CZT5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_013054</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="JAMZMK010006093">
        <property type="protein sequence ID" value="KAI7750752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001023">
        <property type="entry name" value="Os07g0541700 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000299">
        <property type="entry name" value="Receptor-like serine/threonine-protein kinase ALE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47989">
        <property type="entry name" value="OS01G0750732 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47989:SF25">
        <property type="entry name" value="PROLINE-RICH RECEPTOR-LIKE PROTEIN KINASE PERK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041937556" description="non-specific serine/threonine protein kinase" evidence="12">
        <location>
            <begin position="26"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="187"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="263"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="160"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="220"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="173"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="220"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="291"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7750752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="508" mass="55702" checksum="D56D2F9B3A51A4D5" modified="2024-05-29" version="1" fragment="single">SINTNCCFLLKQLLILYIIILTVHADSTDNRGRQRIISQPKRRLSEYGPLATSALPPSISHMSEPLMRKSESTPPVSGFSPPLADIAPTQSGAGRLPTGLAQPPLSPRASRISFTSFEASRINSSLYTHKVHLDPQLVGGYELLNLTWFKPIAPAPAPHIATPPKKLPPSMPSSPATSNTSSSGKRLSWVLVVGIGAGILFVAVICVLILCSCASRRRKPESLPKETAKPRTMESLQTAGSFTHPTSTRFLEYEELKAATNNFEHASIVGEGGFGRVFKGVLSDGTHVAIKRLSSGGQQGGKEFLVEVEMLSRLHHRNLVKLVGYHSNRDNSENLLCYELVPNGSLEAWLHGKLFFRNCTFCHNTLCKGCYCLFAGPLGVNCPLDWETRMKIALDAARGLAYLHEDSQPCVIHRDFKASNILLENDFHAKVADFGLAKLAPEGRATXXXXLWEIIYRYVAPEYAMTGHLLVKSDVYSYGVVLLELLTGRKPVDMSQPSGQENLVSWVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D018</accession>
    <name>A0AAD5D018_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Metalloenzyme domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_022486</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the interconversion of 2-phosphoglycerate and 3-phosphoglycerate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R)-2-phosphoglycerate = (2R)-3-phosphoglycerate</text>
            <dbReference type="Rhea" id="RHEA:15901"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="EC" id="5.4.2.12"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Carbohydrate degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the BPG-independent phosphoglycerate mutase family. A-PGAM subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006121">
        <property type="protein sequence ID" value="KAI7750577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D018"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004619">
        <property type="term" value="F:phosphoglycerate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16011">
        <property type="entry name" value="iPGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006124">
        <property type="entry name" value="Metalloenzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004456">
        <property type="entry name" value="Pglycerate_mutase_ApgM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31209">
        <property type="entry name" value="COFACTOR-INDEPENDENT PHOSPHOGLYCERATE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31209:SF0">
        <property type="entry name" value="METALLOENZYME DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01676">
        <property type="entry name" value="Metalloenzyme"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10143">
        <property type="entry name" value="PhosphMutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metalloenzyme" evidence="6">
        <location>
            <begin position="9"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="Metalloenzyme" evidence="6">
        <location>
            <begin position="326"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7750577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="494" mass="52945" checksum="8073CA375F1146B0" modified="2024-05-29" version="1">MVNSDTLRKRVAFVLIDGLGDVSIPRFGYKTPLEAAKVPNLDAIASAGVNGLMDPVEVGLGCGSDTAHLSLLGYDPRVYYRGRGAFESMGAGLAMAPGDIAFKSNFATLDEKTGVVTSRRADRHFEEEGPILCAALDKMKLPSFPEYEVRVRYATEHRCGVVVKGPKLSGNISGTDPLKDNRLLLEVKPLDNTEEAKHTAAVVNELSKEISKILVAHPLNQKRATEGKNIANIVLLRGCGIRIEVPAFQKNHGLRPCMVAPTKIIAGLGLSLGIDILEAPGATGDYRTLLTSKATAIANALSAPMRTCPNVFVPGEDEHKPGVSDGYDFGFLHIKAIDDAGHDKATVFKVKGLEAVDKAIGQLARLLWETESTGQFQFYLCVTGDHSTPVEYGDHSFEPVPFTLCRLKDYVGAVGGESVVSQTSLDTFPLPTVEAGDDLSAYVAAGEEISQAGRAFSGDSVWKLNEIAAARGSLGRFPGSEMMGIIKKFIQLEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D0Y6</accession>
    <name>A0AAD5D0Y6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">SOSEKI DIX-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_028747</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="8">Homodimer. Forms long polymer filaments with other SOKs proteins polymers (e.g. SOK1, SOK2, SOK3 and SOK4) crucial for polar localization and biological activity. Binds to ANGUSTIFOLIA (AN).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the SOSEKI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005918">
        <property type="protein sequence ID" value="KAI7751397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D0Y6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051258">
        <property type="term" value="P:protein polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051302">
        <property type="term" value="P:regulation of cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000067">
        <property type="term" value="P:regulation of root morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090708">
        <property type="term" value="P:specification of plant organ axis polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010369">
        <property type="entry name" value="SOK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048351">
        <property type="entry name" value="SOK_DIX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021182">
        <property type="entry name" value="SOK_magnoliopsida"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31083:SF6">
        <property type="entry name" value="PROTEIN SOSEKI 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31083">
        <property type="entry name" value="UPSTREAM OF FLC PROTEIN (DUF966)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06136">
        <property type="entry name" value="SOK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF031043">
        <property type="entry name" value="UCP031043"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SOSEKI DIX-like" evidence="10">
        <location>
            <begin position="46"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="154"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="229"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="458"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="159"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="229"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="468"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF06136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7751397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="528" mass="58150" checksum="F31AACD1AFEE6B67" modified="2024-05-29" version="1">MEGQVRKYGQKHQQLSPDRAKVWKERSCKYKQLQPEAVQEPKSRKVAVVYYLCKNRQLEHPHFIEVSLSSSEGLFLKDVIEKLNGLRGSGMASMYSWSCKRSYKHGFVWHDLCDDDIIIPVNGNEYVLKGSELVEESNSDGFAPSVKLQNLKQLPEPPSARSQDDSSSSSTSMSVRDTKNSQDDDLSPPVRHSGSSGVSPESKGRKSTASNGGLSLTEYKIYKTDSLADASTQTEENTKANKAQETCTRGVSTDDVVPKLDPSPPCSSGTSSSVGKTETLESLIIADASKYSNFRRLKGSQAQVNSKLKAADMLMQLISCGSISVKDHSFGLIPSYKPGLSDSKFQSPLFSTSVMLRELDCYSDNSRFTSMRLEDKEYFSGSVVDSKLLKEEGTATLKRSSSYNADRTDKGNHVSDKEETLTLIKCIPKSLKPSLSKNSKNEQMKSAAYEGQCISSEAGPSSRIVSPCPSNSSSKRITTDPCSSKKHSGRLESFREEKENAIKVEESLLQELRLSSTLEAVYDTEGNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D1K6</accession>
    <name>A0AAD5D1K6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">La protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_006987</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Binds to the 3' poly(U) terminus of nascent RNA polymerase III transcripts, protecting them from exonuclease digestion and facilitating their folding and maturation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005840">
        <property type="protein sequence ID" value="KAI7751697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D1K6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08030">
        <property type="entry name" value="LA_like_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12291">
        <property type="entry name" value="RRM1_La"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000795">
        <property type="entry name" value="La protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045180">
        <property type="entry name" value="La_dom_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006630">
        <property type="entry name" value="La_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014886">
        <property type="entry name" value="La_xRRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002344">
        <property type="entry name" value="Lupus_La"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22792:SF140">
        <property type="entry name" value="ACHILLES, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22792">
        <property type="entry name" value="LUPUS LA PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05383">
        <property type="entry name" value="La"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08777">
        <property type="entry name" value="RRM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00302">
        <property type="entry name" value="LUPUSLA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00715">
        <property type="entry name" value="LA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50961">
        <property type="entry name" value="HTH_LA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51939">
        <property type="entry name" value="XRRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="HTH La-type RNA-binding" evidence="9">
        <location>
            <begin position="4"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="117"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="XRRM" evidence="10">
        <location>
            <begin position="284"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="194"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="234"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="387"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="251"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="281"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="403"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00057261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7751697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="428" mass="48166" checksum="8FE49952B3D695BF" modified="2024-05-29" version="1">MVSTEFDEETVKKIIRQVEFYFSDSNLPRDNFLRNTISESEDGMVSLGLICSFSRMRGHLGLGDIKVEDVSDETVEAVADILKTKSTTLKISHDGKKVGRTTELLKPEEAIEQLDSRTIAASPFQYDIKLEDVESFFGQSAKVNSVRLPRHVADKRVLCGTALVEFSSEEEATHILTQNLVFRGAKLELKPKKEFDEQRAKEEEVENSRRDRKNASQEEDYPKGLLVAFKLKSTSSEDGSAKPESVAEESVDVKSSSENDEKNTEAKKVSSSENEEETEEDNKNEKKTETKKMSADMYKDDKDVVLREDLKSVFQKFGTVKYIDFKMGEESGYIRFEEAEGAQKARAAAVLTEEGGLIVKNFVAVLDPVTGEAEKEYWNMLRGQEKFRGNKGNWSRGGKNRGGRQSNRNSRDNNSGNRPNKFQKVAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D1L6</accession>
    <name>A0AAD5D1L6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_004350</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005838">
        <property type="protein sequence ID" value="KAI7751712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D1L6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009640">
        <property type="term" value="P:photomorphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044630">
        <property type="entry name" value="SPA1/2/3/4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44218">
        <property type="entry name" value="PROTEIN SPA1-RELATED 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44218:SF6">
        <property type="entry name" value="PROTEIN SUPPRESSOR OF PHYA-105 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="111"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="114"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7751712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="485" mass="53795" checksum="D36BB9D975371E83" modified="2024-05-29" version="1" fragment="single">MDSEVNATNDINVQDKKSGQFTNVSDNKNNDWVGTSSHASTSPRCADNPVVSVEELTLTNFSGGKSEIINAPNIIDGIKDTSTPNPRVWQDSDSNFFSEFIEKKQEIVSQIQNERTDNSPQDEKQPSHGGIRTKIVSKSGFSEFFVKNTLKGKGVIYKGPARDRVSVHSRGQSDIVSFPVITPDPRPSVHPNNNNVSLREWLTTRDSSHSRGVVLEALKPSCFMLFPSNDVLYLGPPANKQLTETNKRRRLGDNRNSFARWLQLPNQVSGYGLNEENVGSDSDSLEAQWYAGPEDGRERCSTLSSNMYSLGVLLFELLASFESGREHVVAMINLRQRILPPSFLSVNPMEAGYCLWLLHPEASARPTTRDILKSKLVSGIEEVSTAGLFSSIVQEDTESDLLLHFLASLEEQKQKCATKLIEDINCIESDISEIESRRANNSTFETNVTSSSVIKTNLIHLEKAYFSVRSGINVSENDTTGISDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D2D9</accession>
    <name>A0AAD5D2D9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TF-B3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_000055</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005584">
        <property type="protein sequence ID" value="KAI7752896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D2D9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd10017">
        <property type="entry name" value="B3_DNA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.330.10">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003340">
        <property type="entry name" value="B3_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015300">
        <property type="entry name" value="DNA-bd_pseudobarrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044837">
        <property type="entry name" value="REM16-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31391:SF106">
        <property type="entry name" value="B3 DOMAIN-CONTAINING PROTEIN OS01G0723500"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31391">
        <property type="entry name" value="B3 DOMAIN-CONTAINING PROTEIN OS11G0197600-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02362">
        <property type="entry name" value="B3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM01019">
        <property type="entry name" value="B3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101936">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50863">
        <property type="entry name" value="B3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TF-B3" evidence="6">
        <location>
            <begin position="21"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="TF-B3" evidence="6">
        <location>
            <begin position="224"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="TF-B3" evidence="6">
        <location>
            <begin position="433"/>
            <end position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7752896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="552" mass="61569" checksum="D9A600E2D39B0323" modified="2024-05-29" version="1">MNAKPPHFIKGFNPNISIHKLKIPSKFVKHLEGKTAGTVYLTGPSGNTWHADLAQETDGLFILNGWASFVRDHFLENGDSLVFRYDGNLHFTLQIFDLSSCEKETAFSAECHQDLSIFDQHFGKKREREYASLFTNTADCVPKKPRSSQAAHEMQLGLQESINGQCEVADFLSNADFCSSALKSPLNTVMPISEVCPNDDELGRLSASEADKIAQSFTSSFPHFTQVMKRFNISGSYTMNVPYQFAMAYLPNCKVKIVLHNLKGESWTVNSIPTTRVQTSHTFCGGWLAFVRGNNINIRDICIFELVGKCEMRVNILRVRQELLDYEHSDLKGSTNGASHKVSGRLTKKVKRKSLKTALTESQKFAFSIEKVKHGIAAKGSVGSQLRTINGRSGNARVFQENRGSSMTGFTSMKSAPEEKIAAESFVSSFPYFVRVMKKFNIGGSYTLKIPFRFSMEHLPNFRTEIILHNLKGECWTVNSVPTLKVQTLHTICGGWMAFVRDNDIRLGDICIFELVGKCEMRVHITSVGKTGINLDYQSGTGPSNELANVSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-02-05" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D429</accession>
    <name>A0AAD5D429_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Large ribosomal subunit protein uL15/eL18 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_023567</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005175">
        <property type="protein sequence ID" value="KAI7753893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.100.10.10:FF:000001">
        <property type="entry name" value="60S ribosomal protein L18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.100.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000039">
        <property type="entry name" value="Ribosomal_eL18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021131">
        <property type="entry name" value="Ribosomal_uL15/eL18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036227">
        <property type="entry name" value="Ribosomal_uL15/eL18_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10934">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10934:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17135">
        <property type="entry name" value="Ribosomal_L18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52080">
        <property type="entry name" value="Ribosomal proteins L15p and L18e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL15/eL18" evidence="5">
        <location>
            <begin position="2"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="153"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="159"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="176"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF17135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7753893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="186" mass="20995" checksum="462A38AAEB99F73A" modified="2024-05-29" version="1">MGIDLVAGGKSKRTKRTAPRSDDIYLKLLVKLYRFLVRRTGSNFNAVILKRLFMSKVNKPPISLSRLIRYMSGKEDKIAVVVGTVTDDVRVHEIPCMKVTALRFTETARARIEKAGGECLTFDQLALRAPLGQNTVLLRGPKNAREAVKHFGPAXXXHSHTKPYVRSKGRKFEKARGRRNSRGFRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D594</accession>
    <name>A0AAD5D594_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_011663</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005552">
        <property type="protein sequence ID" value="KAI7753094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D594"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="23"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7753094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="45" mass="5238" checksum="8BC4336E1D4C9C6D" modified="2024-05-29" version="1">MYLAGANQHQLPPNGFEYYSRSFYFTFNALLVPLISFVHDALVVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D5G7</accession>
    <name>A0AAD5D5G7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_024732</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase A1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005301">
        <property type="protein sequence ID" value="KAI7753719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D5G7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05476">
        <property type="entry name" value="pepsin_A_like_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034161">
        <property type="entry name" value="Pepsin-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13683:SF822">
        <property type="entry name" value="ASPARTIC PEPTIDASE A1 FAMILY, ASPARTIC PEPTIDASE DOMAIN, XYLANASE INHIBITOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13683">
        <property type="entry name" value="ASPARTYL PROTEASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="5">
        <location>
            <begin position="76"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="94"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7753719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="455" mass="50137" checksum="1856827C91DC83D7" modified="2024-05-29" version="1">MDLHGRGLVLAIGFVVVQLCMLASANVVFQVHHKFAGRKRYLKALISHDDNRRQRFLSATELPLGGDSLPTAAALYFTKLQIGSPPKDFHVQVDTGSDLLWVNCIECENCPKKSDLGIPLALYDPKASSSAKKVTCDDKFCKATFTDQSNECKAGMLCAYSVKYGDGSATSDRCGAKQSGQLGSSDQALDGILGFGQSNTSMLSQLSSAKKMRKMFSHCLDGKRGGGIFAIGEVVQPKIKTTPLIDEQTHYNIELKSIDVNGEDIKLPTGILDFFQKQAAVVDSGTTLAYFPDEVYNQIMEKIMAAQPGKKPHVVQKRFKCYKYSGNIDDGFPVVNLHFANSLSLKVLPHQYFFQVQDDEWCIGFQDSNLKGNTGKEITLLGDIVLSDKLVTYDMEKKVIGWIDYNCSSSIKVKDEESGKVYEVVAKDISSSHHRSTSRMILGLLLFLTATFLIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D6P2</accession>
    <name>A0AAD5D6P2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_023004</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010002366">
        <property type="protein sequence ID" value="KAI7754863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D6P2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7754863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="143" mass="16467" checksum="313ED372C88E9EEC" modified="2024-05-29" version="1" fragment="single">MINEEGFTHTAIKTKKGNKSVNERIHDERRPLLKPKKGNKSVKERIDDEGVAKTAVVYNTWKVNEDDLRENIKKCRKAKARSKDVIRARTSPNKSMGFRKIPKLKMENIPGSLGLYILGQLDTDKMRINVNKEHLDVDIESIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D8R0</accession>
    <name>A0AAD5D8R0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">UDP-arabinopyranose mutase</fullName>
            <ecNumber evidence="4 9">5.4.99.30</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_023299</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Probable UDP-L-arabinose mutase involved in the biosynthesis of cell wall non-cellulosic polysaccharides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>UDP-beta-L-arabinofuranose = UDP-beta-L-arabinopyranose</text>
            <dbReference type="Rhea" id="RHEA:28350"/>
            <dbReference type="ChEBI" id="CHEBI:61457"/>
            <dbReference type="ChEBI" id="CHEBI:61463"/>
            <dbReference type="EC" id="5.4.99.30"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the RGP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.30" evidence="4 9"/>
    <dbReference type="EMBL" id="JAMZMK010000694">
        <property type="protein sequence ID" value="KAI7755928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D8R0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009505">
        <property type="term" value="C:plant-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052691">
        <property type="term" value="F:UDP-arabinopyranose mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071669">
        <property type="term" value="P:plant-type cell wall organization or biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033356">
        <property type="term" value="P:UDP-L-arabinose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00761">
        <property type="entry name" value="Glyco_tranf_GTA_type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004901">
        <property type="entry name" value="RGP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037595">
        <property type="entry name" value="RGP_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31682:SF45">
        <property type="entry name" value="UDP-ARABINOPYRANOSE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31682">
        <property type="entry name" value="UDP-ARABINOSE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03214">
        <property type="entry name" value="RGP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016429">
        <property type="entry name" value="UPTG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6 11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="site" description="Required for activity" evidence="10">
        <location>
            <position position="152"/>
        </location>
    </feature>
    <feature type="site" description="Required for activity" evidence="10">
        <location>
            <position position="159"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (Glc...) arginine" evidence="11">
        <location>
            <position position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR016429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR016429-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR016429-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7755928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="365" mass="41244" checksum="A17F1F7E70769FDE" modified="2024-05-29" version="1">MSVPATTVTPPLADDLDIVIPTIRNLDFLEQWRSFFQPYHLIIVQDGDPSKTIRVPDGFDYELYNRNDINRILGTKASCISFKDSACRCFGFLVSKKKYIFTIDDDCFVAKDPSGQEINALAQHIHNLLTPSTPYFFNTLYDPYREGADFVRGYPFSLREGVTTAISHGLWLNIPDYDAPTQLVKPLERNSRYVDAVLTIPKGTLFPMCGMNLGFHRELIGPAMYFGLMGDGQPIGRYDDMWAGWCAKVICDHLGLGVKTGLPYIWHSKASNPFVNLKKEYKGIYWQEEIIPFFQSVILPKDCTTPQKCYVELAKLVKEKLGPIDPYFGKLSGEMVTWVNAWNELNPSTDVAAVKNIDAAAPKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D9Z5</accession>
    <name>A0AAD5D9Z5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">CASP-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_026682</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="7">Homodimer and heterodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cell membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the Casparian strip membrane proteins (CASP) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010002287">
        <property type="protein sequence ID" value="KAI7754896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D9Z5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006459">
        <property type="entry name" value="CASP/CASPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006702">
        <property type="entry name" value="CASP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044173">
        <property type="entry name" value="CASPL"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01569">
        <property type="entry name" value="A_tha_TIGR01569"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36488:SF11">
        <property type="entry name" value="CASP-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36488">
        <property type="entry name" value="CASP-LIKE PROTEIN 1U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04535">
        <property type="entry name" value="CASP_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="49"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="94"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="135"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="185"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="Casparian strip membrane protein" evidence="9">
        <location>
            <begin position="47"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7754896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="207" mass="21890" checksum="507B66753083FD24" modified="2024-05-29" version="1">MEGDSTTINITETPKERKGKAPLLATPPASSSIKPVVQKAPKRGYKRGLAILDVVLRIAGIAAALGAAIAMASTEETLPFFTQFFQFQAQYDDLPAFTFFVIANSITAAYLAVSIPISIVCIVRPHLVAPRIILIFLDTVMVALTTAAAGGAASIVYLAHNGNSDANWPAICQQFSDFCQEVSGAVVASFLTVVVLMVLVVLSAFSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DB52</accession>
    <name>A0AAD5DB52_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">PA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">M8C21_006713</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">AA19_3_7</strain>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Intramembrane-cleaving aspartic protease (I-CLiP) that cleaves type II membrane signal peptides in the hydrophobic plane of the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endosome membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase A22B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000346">
        <property type="protein sequence ID" value="KAI7756554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DB52"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098554">
        <property type="term" value="C:cytoplasmic side of endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030660">
        <property type="term" value="C:Golgi-associated vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098553">
        <property type="term" value="C:lumenal side of endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042500">
        <property type="term" value="F:aspartic endopeptidase activity, intramembrane cleaving"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033619">
        <property type="term" value="P:membrane protein proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.30:FF:000007">
        <property type="entry name" value="Signal peptide peptidase-like 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007369">
        <property type="entry name" value="Peptidase_A22B_SPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006639">
        <property type="entry name" value="Preselin/SPP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12174">
        <property type="entry name" value="SIGNAL PEPTIDE PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12174:SF90">
        <property type="entry name" value="SIGNAL PEPTIDE PEPTIDASE-LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04258">
        <property type="entry name" value="Peptidase_A22B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00730">
        <property type="entry name" value="PSN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0967">Endosome</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0732">Signal</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042106887" description="PA domain-containing protein" evidence="13">
        <location>
            <begin position="37"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="194"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="250"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="273"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="308"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="342"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="369"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="424"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="460"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="489"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="domain" description="PA" evidence="14">
        <location>
            <begin position="87"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAI7756554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="532" mass="58122" checksum="3AE5572E79C8508C" modified="2024-05-29" version="1">MKMRNRNRNCNPSSIIPILTLIYAILFSTLSPTATADGSLIFCDNTSVSVMIKVWVDGVEGKTLVGQSATFSAKIPKTANEIAKQSVVLTNPLNCCSNLSSELSGFTGLARRGDCDYLVKAKVAQAAGAAGLVVINDSEDIVEMSCSEKTALDISIPVVMISKHGGEALNKSLSSGGKVELLVYAPESPIIDPGAAFLWLMAVATVVCASLWSEITKSRETNGNYDELSPKSNHKDDEENEVIEMSTTSAVVFVISASTFLVLLYFFMSSWFIWVLIVFFCLGAIEGMHSCIVALVKSKCRGCGQRTVNVPFFGTTTVMSLLVLLFCMAFAIFWVWTRKESYSWVGQDILGICLIIAVLQLAQLPNVKVATVLLCCAFMYDIFWVFISPLFFNDSVMISVAQGDSSSGESIPMVLRFPRLFDPWGGYSVLGFGDILFPGLLLSFTVRYDKAKKRTWQDGYFVWLAIGYACGLLLTYLALYLMNGHGQPALLYLVPCTLGTCIILGLVRGELKELWNYNADESKQTLVGDGQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DC38</accession>
    <name>A0AAD5DC38_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">O-acyltransferase WSD1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_030647</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000279">
        <property type="protein sequence ID" value="KAI7756724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DC38"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008374">
        <property type="term" value="F:O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019432">
        <property type="term" value="P:triglyceride biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045034">
        <property type="entry name" value="O-acyltransferase_WSD1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009721">
        <property type="entry name" value="O-acyltransferase_WSD1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31650">
        <property type="entry name" value="O-ACYLTRANSFERASE (WSD1-LIKE) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31650:SF34">
        <property type="entry name" value="O-ACYLTRANSFERASE WSD1-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06974">
        <property type="entry name" value="WS_DGAT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="O-acyltransferase WSD1 C-terminal" evidence="1">
        <location>
            <begin position="43"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7756724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="197" mass="22159" checksum="FAA74A84804E2FD9" modified="2024-05-29" version="1">MEETCEEAKNARSTALVLLNTRSIGGYKSVNEMVGNQEAQVQWGNQFGFLHVSLPKLNEYDQCLNPLKFVQETQNIIKRKRNSSAVFLTGMLLESVRKYIGPEATAKYIHNTLKNSSMSVSNMIGPVEKMTLANQPVKGLYFMVVNGPQSLTVTIMSYMDQLKVAFGTDKGHIDQENFRKYIEKAFSIMYNTAVVAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DC85</accession>
    <name>A0AAD5DC85_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Hexosyltransferase</fullName>
            <ecNumber evidence="12">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_029477</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>myo-inositol + UDP-alpha-D-galactose = alpha-D-galactosyl-(1-&gt;3)-1D-myo-inositol + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12464"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17268"/>
            <dbReference type="ChEBI" id="CHEBI:17505"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:66914"/>
            <dbReference type="EC" id="2.4.1.123"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyltransferase 8 family. Galactosyltransferase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="12"/>
    <dbReference type="EMBL" id="JAMZMK010000178">
        <property type="protein sequence ID" value="KAI7757124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DC85"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047216">
        <property type="term" value="F:inositol 3-alpha-galactosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070417">
        <property type="term" value="P:cellular response to cold"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006012">
        <property type="term" value="P:galactose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02537">
        <property type="entry name" value="GT8_Glycogenin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000049">
        <property type="entry name" value="Hexosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002495">
        <property type="entry name" value="Glyco_trans_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050587">
        <property type="entry name" value="GNT1/Glycosyltrans_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11183">
        <property type="entry name" value="GLYCOGENIN SUBFAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01501">
        <property type="entry name" value="Glyco_transf_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0299">Galactose metabolism</keyword>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00050449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU362027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7757124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="315" mass="36219" checksum="FEE8844D4DE3E426" modified="2024-05-29" version="1" fragment="single">AYVTFLAGNGDYVKGVVGLAKGLRKVNSGYPLVVAVLPDVPVEHRRVLEAQGCVVREIQPVYPPESQTQFAMAYYVINYSKLRIWEFVEYEKMIYLDGDIQVFDNIDHLFDLPDGYFYAVMDCFCEKTWSHTPQYKIGYCQQSPERVRWPKDMGLKPPLYFNAGMFVFEPSIDTYHDLLNTLRVTTPTSFAEQDFLNLYFKDVYKPIPLIYNLVLAMLWRHPENVDLEQVKVVHYCAAGSKPWRYTGKEENMQREDIKMLVKKWWDIYNDKSLDYMNPNGNMAAHVTTGRGSNGEMVLPSALTDPKVVVCAPSAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DCG4</accession>
    <name>A0AAD5DCG4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_028786</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000254">
        <property type="protein sequence ID" value="KAI7756810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DCG4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010333">
        <property type="term" value="F:terpene synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.130">
        <property type="entry name" value="Terpene synthase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001906">
        <property type="entry name" value="Terpene_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036965">
        <property type="entry name" value="Terpene_synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050148">
        <property type="entry name" value="Terpene_synthase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005630">
        <property type="entry name" value="Terpene_synthase_metal-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31225">
        <property type="entry name" value="OS04G0344100 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31225:SF137">
        <property type="entry name" value="TERPENE SYNTHASE 11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01397">
        <property type="entry name" value="Terpene_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03936">
        <property type="entry name" value="Terpene_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Terpene synthase N-terminal" evidence="3">
        <location>
            <begin position="10"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="Terpene synthase metal-binding" evidence="4">
        <location>
            <begin position="199"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7756810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="468" mass="54272" checksum="12EC91D16BBE1A31" modified="2024-05-29" version="1">MKVIPQRVDVSKRMDELRENTKRALMVTSDPKMTLNLIDTIQRLGIGHCFKEEINNILEDLTQVLSDDDLYTVALNFCLRRHNGLDSNPAFFQIFMEQNGELKKSSTEDIEGLLSSSGENVLSHLREITTRHLSSSISQLSTKLHKKLPRHMRMERLEARGYIEEYGNEDDHNPIVLEFAKYKYNKVQSTFQMELVEEHLGLSAKLPFVRDRHIECFLWIVGLLPEPSFSGSRIELAKAIAIMLVIDDIYDTYGSYDDLVPFTEAIQRWDLNEMEQLPEYMKICFKALYNTNNEICVKVLSERGLSVQPFLCKTWIEMVKAYMVEVEWVKRGRTLTIKDYIKNGVTTFGTYMALVHLFFLVSNGLTIENMQHLLDPYPKFFTVAGTILRGTEKRGYVIKHTFTHEREKHNIGEERNITVTQDVVLFPIIKVAVNMSRASQVVYKHNDDSYLLSVEHHVQSLFYKPVDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DCR1</accession>
    <name>A0AAD5DCR1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_034053</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000184">
        <property type="protein sequence ID" value="KAI7757099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DCR1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7757099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="50" mass="5699" checksum="462BB37933794B83" modified="2024-05-29" version="1" fragment="single">MTPNFSVLEDLFSGKTRHKWNHDGPAEFGVMGHMRFGSQWIQSIGYGSKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DDS4</accession>
    <name>A0AAD5DDS4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_029123</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000107">
        <property type="protein sequence ID" value="KAI7757522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DDS4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7757522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="96" mass="10979" checksum="2548410513681A4A" modified="2024-05-29" version="1" fragment="single">DNFKDLTLSPSTPSTHTTPLFTPSINWFSYKTTGSPPPTSIHQHLQLPSPYRSPFVVDEVACRRIWPIKIFGSSDLFTLDLYSYLFRSMDDVKVIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DE97</accession>
    <name>A0AAD5DE97_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Chitin-binding type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_029183</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Defense against chitin-containing fungal pathogens.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 19 family. Chitinase class I subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000057">
        <property type="protein sequence ID" value="KAI7757807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DE97"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004568">
        <property type="term" value="F:chitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016998">
        <property type="term" value="P:cell wall macromolecule catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00325">
        <property type="entry name" value="chitinase_GH19"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00035">
        <property type="entry name" value="ChtBD1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.20.10:FF:000001">
        <property type="entry name" value="Endochitinase (Chitinase)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.530.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.20.10">
        <property type="entry name" value="Endochitinase, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.10">
        <property type="entry name" value="Endochitinase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001002">
        <property type="entry name" value="Chitin-bd_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018371">
        <property type="entry name" value="Chitin-binding_1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036861">
        <property type="entry name" value="Endochitinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000726">
        <property type="entry name" value="Glyco_hydro_19_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22595:SF194">
        <property type="entry name" value="CHITINASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22595">
        <property type="entry name" value="CHITINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00187">
        <property type="entry name" value="Chitin_bind_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00182">
        <property type="entry name" value="Glyco_hydro_19"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00270">
        <property type="entry name" value="ChtBD1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="Lysozyme-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57016">
        <property type="entry name" value="Plant lectins/antimicrobial peptides"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00026">
        <property type="entry name" value="CHIT_BIND_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50941">
        <property type="entry name" value="CHIT_BIND_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00773">
        <property type="entry name" value="CHITINASE_19_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00774">
        <property type="entry name" value="CHITINASE_19_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5" id="KW-0146">Chitin degradation</keyword>
    <keyword evidence="3 7" id="KW-0147">Chitin-binding</keyword>
    <keyword evidence="6 7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0611">Plant defense</keyword>
    <keyword evidence="5" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042265552" description="Chitin-binding type-1 domain-containing protein" evidence="8">
        <location>
            <begin position="27"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-1" evidence="9">
        <location>
            <begin position="26"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="31"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="36"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7757807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="505" mass="55498" checksum="58CAAF8591381A4E" modified="2024-05-29" version="1">MKTNILSLLFTCIFLLGITFFEPVASQKCKCAPNMCCSKYGYCGTSSAYCGKDCRGGPCSLPAPNNNANVAGIVTISFFNNIVYKSRGNCPGRRFYTRDAFLKAIKNYPHFGRSGSIADSKREIAAFFAHVTRVTGHFCYIEEIKGSSRNYCDMTATKYPCNPKKSYYGRGPIQLAWNYNYGEVGKSIGFDGLNNPEIVARDPVVSFKTALWFWMENVHWDFASGKGFGATIKAVNGNECNGKKRASVSARVAYYIGYCKQFGVPAGKKLSQWGYCGTSSAYCGKGCQSGPCTRPPPNNNANVASIVTVSFFNSIGAMSAKGCPARGFYTRNAFLKAIKAYPRFGRTGSLADSKREIAAFFAHVTHETGHFCYKEEIGGHSKTYCDKSSTKYPCNPKKRYYGRGPFQLTWNYNYGKAGKTLGFDGLNNPDIVARDPVISFKTALWFWMNNIHSGFTSGKGFGATIRAINGGECNGHMPAAVRARVKYYTAYCKKFGVSPGKNLSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DFG6</accession>
    <name>A0AAD5DFG6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_014456</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000259">
        <property type="protein sequence ID" value="KAI7756785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DFG6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000294">
        <property type="entry name" value="Transformation/transcription domain associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050517">
        <property type="entry name" value="DDR_Repair_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046805">
        <property type="entry name" value="Tra1_ring"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11139">
        <property type="entry name" value="ATAXIA TELANGIECTASIA MUTATED ATM -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11139:SF1">
        <property type="entry name" value="TRANSFORMATION_TRANSCRIPTION DOMAIN-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20206">
        <property type="entry name" value="Tra1_ring"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041966055" evidence="1">
        <location>
            <begin position="20"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7756785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="777" mass="86616" checksum="4870B18D40F7A2C0" modified="2024-05-29" version="1" fragment="single">MAEKSVFKILLMTIIAASAEPDLADPKDEYVANICHHFAIIFHLENSSTNAPVSAIPLGGPLLSSNTSNSLKQRNGNSSNLKELDPLIFLDALVEVLADENRLHAKAALNALNIFSETLLLLARSKHADVLMSRGGPGTPMIVSSPSMSPVYSPPPSVRVPIFDQLLPRLLHCCYGSTWQAQMGGVIGLGALVGKVTVETLCVFQMRVVRGLVFVLKRLPVYAAKEQEETSQVLTQVLRVVNNVDEANNEARRQSFQGVVEFLASELLNTNATVRVSKVVQSCLALLASRTGSEVSELLEPLYQPLLQSLLMRPLRSKNVDQQVGTVTALNFCLALRPPLLKLTPELVNFLQEALQIAEADETVWVAKFMNPKVAMSLTKLRTACIELLCTAMAWADFKTQNHSELRAKIISMFFKSLTSRTPEIVAVAKEGLRQVIQQQRMPKELLQSSLRPILVNLAHTKNLNMPLLQGLARLLELLSNWFNVALGSKLLEHLKRWLEPEKLQQIQKSWKSGEEPKIAAAIIELFHLLPSAAGKFLDELVTLTIDLEGALPPGQFYSEINSPYRLPLTKLMYIIRSDAGQPLREELAKSPEKILSSAFPEFIPKAETSMAQGSLETSSLEGDDNAQPQSNPAAPNVLPDAYFQGLALVKTLVKLMPGWLQSNCIVFDSLVLLWKSPARISRLQNKQEQSLVQVKESKWLVKCFLNYLRHDKNEVNVLFEILSIFLYHTRIDFTFLKEFYIIEVAEGYPPSMKKTLLLHFLDIFQSKQMGHDHLVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DIV7</accession>
    <name>A0AAD5DIV7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_013736</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000005">
        <property type="protein sequence ID" value="KAI7758375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DIV7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7758375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="43" mass="4784" checksum="C919C1BC6500179E" modified="2024-05-29" version="1">MVKATASTKHKPPTARYAMPMKLFFPPSQLAVESTSFFLPSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G3A9</accession>
    <name>A0AAD5G3A9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Chitin-binding type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_021774</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Defense against chitin-containing fungal pathogens.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 19 family. Chitinase class I subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011762">
        <property type="protein sequence ID" value="KAI7726073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G3A9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004568">
        <property type="term" value="F:chitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016998">
        <property type="term" value="P:cell wall macromolecule catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00325">
        <property type="entry name" value="chitinase_GH19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00035">
        <property type="entry name" value="ChtBD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.20.10:FF:000001">
        <property type="entry name" value="Endochitinase (Chitinase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.530.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.20.10">
        <property type="entry name" value="Endochitinase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.10">
        <property type="entry name" value="Endochitinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001002">
        <property type="entry name" value="Chitin-bd_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018371">
        <property type="entry name" value="Chitin-binding_1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036861">
        <property type="entry name" value="Endochitinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016283">
        <property type="entry name" value="Glyco_hydro_19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000726">
        <property type="entry name" value="Glyco_hydro_19_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22595">
        <property type="entry name" value="CHITINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22595:SF193">
        <property type="entry name" value="ENDOCHITINASE EP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00187">
        <property type="entry name" value="Chitin_bind_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00182">
        <property type="entry name" value="Glyco_hydro_19"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001060">
        <property type="entry name" value="Endochitinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00270">
        <property type="entry name" value="ChtBD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="Lysozyme-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57016">
        <property type="entry name" value="Plant lectins/antimicrobial peptides"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00026">
        <property type="entry name" value="CHIT_BIND_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50941">
        <property type="entry name" value="CHIT_BIND_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00773">
        <property type="entry name" value="CHITINASE_19_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00774">
        <property type="entry name" value="CHITINASE_19_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5" id="KW-0146">Chitin degradation</keyword>
    <keyword evidence="3 9" id="KW-0147">Chitin-binding</keyword>
    <keyword evidence="6 8 9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0611">Plant defense</keyword>
    <keyword evidence="5" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041939621" description="Chitin-binding type-1 domain-containing protein" evidence="10">
        <location>
            <begin position="23"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-1" evidence="11">
        <location>
            <begin position="22"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="126"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8 9">
        <location>
            <begin position="27"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8 9">
        <location>
            <begin position="32"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="82"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="144"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="230"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001060-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001060-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7726073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="262" mass="28456" checksum="87DD779ACC7DD6BB" modified="2024-05-29" version="1">MKTYGILIFSGVLLAGLLNSAAQNCGCSPDLCCSQWGYCGTGDPYCGPGCREGPCYTSDVDVGSIVTDAFFNGIVDQSDSSCEGRGFYTRATFLEALGNYAEFGTASSEEGSRREIAAFFAHVTHETGHFCYINEINGASRDYCDERYPQYPCNPDKYYYGRGPLQLSWNYNYGAAGNAIGFDGLNNPEIVGTNPLISFQTALWFWMNNVHSIIGQGFGATIRAINGMECNGGNPGTVSARVQYYTQYCSELGVTPGDNLWC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G4S6</accession>
    <name>A0AAD5G4S6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_001830</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011234">
        <property type="protein sequence ID" value="KAI7728352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G4S6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7728352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="116" mass="13176" checksum="E6C6AD5A269EA9AA" modified="2024-05-29" version="1">MAANITSSFFFMHPNLNPIKKSSKSVLMLANKDSPNTSSSLIRVGSPIKIKKVFEDKSRGIMIMGRSHVKDLMKGLACIKVLQDFRVIREEIKILLVFLKGVCLKLQMVRWRENPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G5F6</accession>
    <name>A0AAD5G5F6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA topoisomerase (ATP-hydrolyzing)</fullName>
            <ecNumber evidence="3">5.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_029263</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP-dependent breakage, passage and rejoining of double-stranded DNA.</text>
            <dbReference type="EC" id="5.6.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.2" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010010982">
        <property type="protein sequence ID" value="KAI7729535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G5F6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003918">
        <property type="term" value="F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000712">
        <property type="term" value="P:resolution of meiotic recombination intermediates"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000819">
        <property type="term" value="P:sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.670:FF:000001">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.670">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050634">
        <property type="entry name" value="DNA_Topoisomerase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013760">
        <property type="entry name" value="Topo_IIA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013759">
        <property type="entry name" value="Topo_IIA_B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001154">
        <property type="entry name" value="TopoII_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031660">
        <property type="entry name" value="TOPRIM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10169:SF38">
        <property type="entry name" value="DNA TOPOISOMERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10169">
        <property type="entry name" value="DNA TOPOISOMERASE/GYRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16898">
        <property type="entry name" value="TOPRIM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01158">
        <property type="entry name" value="TOPISMRASEII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00418">
        <property type="entry name" value="TPI2FAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56719">
        <property type="entry name" value="Type II DNA topoisomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="Toprim" evidence="9">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7729535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="153" mass="17854" checksum="5B14FC6D25A93B18" modified="2024-05-29" version="1" fragment="single">HGKVYDNVKSLRYGHLMIMADQDHDGSHIKGLLINFLHSFWPSLLKVPEFVLEFITPIVKATNKKTKNVIAFYTMPEYEAWKENLGIRAREYKIKYYKGLGTSNGKEGAEYFADLERHKKDFIWADDEDGDAIELAFSKKKIEARKNWLRALQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-10-08" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G5Q6</accession>
    <name>A0AAD5G5Q6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">PABC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_016548</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the polyadenylate-binding protein type-1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011200">
        <property type="protein sequence ID" value="KAI7728501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034450">
        <property type="term" value="F:ubiquitin-ubiquitin ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090263">
        <property type="term" value="P:positive regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1900.10:FF:000003">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1900.10">
        <property type="entry name" value="c-terminal domain of poly(a) binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036053">
        <property type="entry name" value="PABP-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002004">
        <property type="entry name" value="PABP_HYD_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46276">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE UBR5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46276:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE UBR5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00658">
        <property type="entry name" value="MLLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00517">
        <property type="entry name" value="PolyA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63570">
        <property type="entry name" value="PABC (PABP) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51309">
        <property type="entry name" value="PABC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="PABC" evidence="9">
        <location>
            <begin position="113"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="36"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="49"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7728501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="210" mass="22401" checksum="D2554F84E7A3E30A" modified="2024-05-29" version="1" fragment="single">XXXXPPQPGFGYQQQLVPGMRPGGGPMPNFFMPMVPPGQQGQRPGGRRVGVPGQQNQQQPVPFMQPQMVPRGRMYRYPPGRNIGDVPMGSVPYDVGNPMHLRDAGGVSSQPIPIGALASALANASPVEQRTMLGENLYPLVEQLEAESAAKVTGMLLEMDQTEVLHLLESPEALKAKVAEAMEVLKSVSQQQGSPADQLAALSLNDGIVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G643</accession>
    <name>A0AAD5G643_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_022057</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010844">
        <property type="protein sequence ID" value="KAI7730089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G643"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34112:SF12">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34112">
        <property type="entry name" value="C-JUN-AMINO-TERMINAL KINASE-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="95"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="340"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="422"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="15"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="422"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="447"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="466"/>
            <end position="485"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7730089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="562" mass="59826" checksum="DCDAE2DF27D5F8B3" modified="2024-05-29" version="1">MERSEPTFVPEWLKSSGSGSVSTTSQQQSQLSSLRSDEQVVSKGIRCKSHVNASDSDLGRASVLERTTSSYFRRPSSSNGSSHLRSYGSFGRYSDKEKLESRHRDYSDPLGNILPSRFEKEGLRHSHSSVSAKRGEIWPRKTVADSTGANKNIHSHSNGGALRSAGGISGVKIAFERDFPSLGSEDKQTDAEIGRVPSPGLSSAIQSLPIGNSAVIGGDGWTSALAEVPVIVSSNGSNTSVVPLTPVSATASMTTGRNMAETLAQGPPRAQTAPQLSAGTQRLEELAVKQSRQLIPMTPSMPKALALNSSDKPKAKVAQLQFDSIKTSPVGKLHVLKPSRERNGATPIAKDNLSPTATGKLPNSPLGVPSAVGPAPPLRNNPAVAVAERKPGVPILEKRPSPQAQSRNDFFNLMRKKSLTSVTPDTGTAISASDKPNEAEISAVTEGGSQRSSESTACNGDADVATEGSTSTTNNNGKTNLNSNGILYSEEEEARLLRLFGWEETAEEEEGLTEEEISSFYRDVSKYLDSRAASKIFKGPQPKLLMTLGKNGDIRPDSKMES</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G6J9</accession>
    <name>A0AAD5G6J9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein DETOXIFICATION</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Multidrug and toxic compound extrusion protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_020209</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010615">
        <property type="protein sequence ID" value="KAI7730840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G6J9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13132">
        <property type="entry name" value="MATE_eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045069">
        <property type="entry name" value="MATE_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11206">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="65"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="97"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="139"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="172"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="202"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="230"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="277"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="316"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="358"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="432"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="458"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7730840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="559" mass="60765" checksum="614B6FE4678F8BAD" modified="2024-05-29" version="1">MCNPTQSSPSKVVVYKNTLFKNPNSNINPPKSMDNNNHHQLHGWPTPSQALEEIKAIGKISGPTALTGLLLYSRAMISMLFLGYLGELELAGGSLSIGFANITGYSIISGLAMGMEPICGQAFGAKQMKLLGLTLQRTVLLLLSTSLPITFIWLNMKTILIWCGQDKEISNVAQTFIVFSIPDLFVLSFLHPLRIYLRTQNITLPLTCCSAVSILIHVPLNFLLVGYFKLGIIGVAVAVVITNLNLLFLLATFVYFSGIHRESWVPPSTDCLNGWSSLLALAIPTCVSVCLEWWWYELMILLCGLLSNPRATVASMGILIQTTSLVYVFPSALSLGVSTRVGNELGAKRPAKARISMIVSLVCAIAMGFIAMVFTTLMRHRWGRFFTKDVEILELTSVALPVVGLCELGNCPQTTGCGVLRGSARPTIGANINLGSFYFVGMPVAILMGFAVKLGFAGLWFGLFAAQVTCALLMLYVLCTTDWIVQVERANELAKPPSSSSSTFSCLVPNSPLPTTIKNEVKNSIEANLEEIRLISSDKEVELVKADCSSLETNPLIVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G7N7</accession>
    <name>A0AAD5G7N7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Vacuolar protein sorting-associated protein 55 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_006140</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the OB-RGRP/VPS55 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010431">
        <property type="protein sequence ID" value="KAI7731559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G7N7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032511">
        <property type="term" value="P:late endosome to vacuole transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007262">
        <property type="entry name" value="Vps55/LEPROT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12050">
        <property type="entry name" value="LEPTIN RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12050:SF0">
        <property type="entry name" value="RH04491P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04133">
        <property type="entry name" value="Vps55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="24"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="58"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="82"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7731559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="113" mass="12522" checksum="CE4D4B9419FC00AA" modified="2024-05-29" version="1">MFSSSLLLQILACAIYNNWWPMLSALMYVLVPMPCMFFGGGSTEYLLSRDGGGWIDAAKFLTGASAVGSIAIPIILRHAHLISTGAMMIEFMSFFIFVGTVMCFHRANLEDEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G848</accession>
    <name>A0AAD5G848_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Kinesin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_030182</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010344">
        <property type="protein sequence ID" value="KAI7731994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G848"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005875">
        <property type="term" value="C:microtubule associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051231">
        <property type="term" value="P:spindle elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969">
        <property type="entry name" value="CHROMOSOME-ASSOCIATED KINESIN KIF4A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969:SF6">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIN-4C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 2" id="KW-0505">Motor protein</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="3">
        <location>
            <begin position="16"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="95"/>
            <end position="102"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7731994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="148" mass="16072" checksum="625AC842872CC596" modified="2024-05-29" version="1" fragment="single">MEGSDAKNNNNNGSQSVRVAVNIRPLVTPELLVGCSDCISVTPGEPQVQIGSHSFTFDYVYGSTGAPSSRIFDDCVEPLVDALFHGYNATVLAYGQTGSGKTYTMGTNYNGECTNGIIPKVMHMIFKRVEDTKGTTEFLIRVSFIEVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G8M7</accession>
    <name>A0AAD5G8M7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_006174</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010065">
        <property type="protein sequence ID" value="KAI7733210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G8M7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38396">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38396:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042042669" evidence="1">
        <location>
            <begin position="22"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7733210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="108" mass="12356" checksum="CD190E063EDC426A" modified="2024-05-29" version="1">MGARICFVILFFWAVLTIVTPILVHLSTKPNLLECYDEEELANFQSGIGLRFWWRRKCQCTEGPLEPISTPAPAPTSAPYDTRTPEFYMMQLTCKGVSRKVLVMVQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GAC3</accession>
    <name>A0AAD5GAC3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_006175</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TUB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010065">
        <property type="protein sequence ID" value="KAI7733211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GAC3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.90.10">
        <property type="entry name" value="Tubby Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025659">
        <property type="entry name" value="Tubby-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000007">
        <property type="entry name" value="Tubby_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16517:SF104">
        <property type="entry name" value="TUBBY-LIKE F-BOX PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16517">
        <property type="entry name" value="TUBBY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01167">
        <property type="entry name" value="Tub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01573">
        <property type="entry name" value="SUPERTUBBY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54518">
        <property type="entry name" value="Tubby C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="4">
        <location>
            <begin position="121"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="Tubby C-terminal" evidence="3">
        <location>
            <begin position="182"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="25"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="32"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7733211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="343" mass="37771" checksum="D375C33C237476F4" modified="2024-05-29" version="1" fragment="single">DGGGVPANPTRETAPFSLINLPDARKLDCRDPSSSPVMQLTTTRSPTRAPKPYKPCISSTNYLNMPFKCLVRGDTEEGMTENKSRNRGQKKNMVSWGRSHIAPVNYDSSMLLPPLINNGGWENLPPELLLDIIHRVEAGKTTWPARRDVVACAAVCRLWRRAIKQVVKTPEQCGLLTFPVSLKQPGPRDEPIQCFIRRDRAASTYRLYLGLSPALAGDASKLLLAAKKVRKATGTDFLISLAANEFSRATNSYVGVLSSNFLGTKFVIYDDQPPYDALIPSNSKFGIKKVTCGALPTVKSKVINISYELNFLRTKGPRRMRCTMNTIPSSAVQQDGTGTWTAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GAQ4</accession>
    <name>A0AAD5GAQ4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_025087</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Heterotetramer of two alpha and two beta chains arranged as a dimer of alpha/beta heterodimers.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009694">
        <property type="protein sequence ID" value="KAI7734559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GAQ4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004298">
        <property type="term" value="F:threonine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000246">
        <property type="entry name" value="Peptidase_T2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10188">
        <property type="entry name" value="L-ASPARAGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10188:SF8">
        <property type="entry name" value="THREONINE ASPARTASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01112">
        <property type="entry name" value="Asparaginase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7734559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="65" mass="7065" checksum="A14636E631F3AC43" modified="2024-05-29" version="1" fragment="single">KWHCVVTNGIKGRGVLGECVDDVSATIEILENNPCTNVGRGANLTEDGYFECYASVMEGNMPEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GDD3</accession>
    <name>A0AAD5GDD3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SWIM-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_010297</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008879">
        <property type="protein sequence ID" value="KAI7738085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GDD3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097196">
        <property type="term" value="C:Shu complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28498">
        <property type="entry name" value="ZINC FINGER SWIM DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28498:SF1">
        <property type="entry name" value="ZINC FINGER SWIM DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50966">
        <property type="entry name" value="ZF_SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SWIM-type" evidence="2">
        <location>
            <begin position="46"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7738085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="116" mass="12952" checksum="2E6F6D2A31116229" modified="2024-05-29" version="1">MDGAVSDDQLAILHILFGKNLERATRIVDEGGVKRILGEPSGRSIFQVVGESRRKEEYLCFPQHYCACYSFFYDIVNRGEQLCCKHQLAARLAVALGTCVDVKVSDDHLAHLLAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GER1</accession>
    <name>A0AAD5GER1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PCI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_008666</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Component of the 19S regulatory particle (RP/PA700) lid subcomplex of the 26S proteasome. The 26S proteasome is composed of a core protease (CP), known as the 20S proteasome, capped at one or both ends by the 19S regulatory particle (RP/PA700). The RP/PA700 complex is composed of at least 17 different subunits in two subcomplexes, the base and the lid, which form the portions proximal and distal to the 20S proteolytic core, respectively.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the proteasome subunit p55 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008600">
        <property type="protein sequence ID" value="KAI7739615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GER1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000070">
        <property type="entry name" value="26S proteasome non-ATPase regulatory subunit 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054559">
        <property type="entry name" value="PSMD12-CSN4-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040134">
        <property type="entry name" value="PSMD12/CSN4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040896">
        <property type="entry name" value="RPN5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10855:SF1">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10855">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 12/COP9 SIGNALOSOME COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22241">
        <property type="entry name" value="PSMD12-CSN4_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18098">
        <property type="entry name" value="RPN5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0647">Proteasome</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="4">
        <location>
            <begin position="212"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00064920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7739615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="422" mass="48456" checksum="98FEDA461AF288AF" modified="2024-05-29" version="1">MRLNGDVAGTKKAAIDILQLCFENRAWKTLNDQIVVLSKRRGQLKQAVTAMVQQAMQYIDETPDIETKIELIKTLNSVSAGKIYVEIERARLIKRLAKIKEEQGQIAEAADLMQEIAVETFGAMAKTEKIAFILEQVRLCLDRQDYVRAQILSRKISPRVFDADTSKEKKKPKEGESIVEEAPADIPSLLELKRIYYELMIRYYSHSNDYLEICRCYKSIYEIPSVKEDSSKWIPVLRKICWYLVLAPHDPMQSSLLNSTLDDKNLSEIPHFRLLLKQLLTMEVIQWTSLWNTFKDEFENEKNMLGGSLGDKAADDLKERVIEHNILVVSKYYARITVKRLAQLLCLTVEEAEKHLSDMVVSKALIAKVDRPMGIVSFQSVKDSNDILNSWATNLEKLLDLVEKSCHQIHKETMVHKAALKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GEU9</accession>
    <name>A0AAD5GEU9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_000516</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008574">
        <property type="protein sequence ID" value="KAI7739750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GEU9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110102">
        <property type="term" value="P:ribulose bisphosphate carboxylase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037494">
        <property type="entry name" value="RAF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040858">
        <property type="entry name" value="Raf1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040781">
        <property type="entry name" value="Raf1_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041358">
        <property type="entry name" value="Raf1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35299">
        <property type="entry name" value="RUBISCO ACCUMULATION FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35299:SF3">
        <property type="entry name" value="RUBISCO ACCUMULATION FACTOR 1.2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18579">
        <property type="entry name" value="Raf1_HTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18578">
        <property type="entry name" value="Raf1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18087">
        <property type="entry name" value="RuBisCo_chap_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rubisco accumulation factor 1 helix turn helix" evidence="5">
        <location>
            <begin position="90"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="Rubisco accumulation factor 1 alpha-helical" evidence="4">
        <location>
            <begin position="162"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="Rubisco accumulation factor 1 C-terminal" evidence="3">
        <location>
            <begin position="292"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="48"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="48"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF18087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF18578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF18579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7739750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="454" mass="50867" checksum="763B957D349749C9" modified="2024-05-29" version="1">MISFTLNPSPPQTTTTFLHTPFLSTPISLTPTHKPIKPHRATISSIIIPSSSNPQQPKQQQQQSQQQKLYQPFRAPLTQSPIPQKYRSLDTTARLDVLTNRLGVWYEYAPLIPPLIQDGFDPSILEEITGISGHEQNTLVVGAQVRESLVQSGVDDYILEFFDKGGAELLYEIRLLTNNQRADAAKYMLENKFEPSDAQELARAIKDYPRRRGDRGWERFDYTIPADCLAFMYYRQALEYRESELRVSALEKALSVAVSDRAKEWITKELEGKIQGGEGQGRGGGVIDGVKVPVVRLNLGEIAEATVVAVFPVCSAEDKIKGVEEAPWEYRSKGDFGIVEADKGWKRWVVLPAWGPVMGLKEGGVAVAFSDAKALPWRVNRWYKEESILVVANRGRKEVTEDDGFYLVSSDDELKIERGATLKEKGCTETLGSVAIVVRPPRESNDELSPEDWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GFW2</accession>
    <name>A0AAD5GFW2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_033049</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008323">
        <property type="protein sequence ID" value="KAI7740922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GFW2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06464">
        <property type="entry name" value="ACD_sHsps-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16100">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.60:FF:000018">
        <property type="entry name" value="AT-rich interactive domain-containing protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.790:FF:000014">
        <property type="entry name" value="AT-rich interactive domain-containing protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.60">
        <property type="entry name" value="ARID DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045147">
        <property type="entry name" value="ARI3A/B/C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001606">
        <property type="entry name" value="ARID_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036431">
        <property type="entry name" value="ARID_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15348:SF17">
        <property type="entry name" value="AT-RICH INTERACTIVE DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15348">
        <property type="entry name" value="AT-RICH INTERACTIVE DOMAIN-CONTAINING PROTEIN ARID DOMAIN- CONTAINING PROTEIN DEAD RINGER PROTEIN B-CELL REGULATOR OF IGH TRANSCRIPTION BRIGHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01388">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01014">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00501">
        <property type="entry name" value="BRIGHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46774">
        <property type="entry name" value="ARID-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51011">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="ARID" evidence="9">
        <location>
            <begin position="134"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="SHSP" evidence="8">
        <location>
            <begin position="343"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="261"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="295"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="34"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="297"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7740922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="444" mass="50057" checksum="64B1A1C5056CC2E1" modified="2024-05-29" version="1">MQANKGPNEIGQDEEETQLNKGPNEIEQGEEEMQDNKIEQESLVDEAHDNGKDGSAMGDDKVVDSKIHAAYMDDDHRGSLKMPESNIEREYTGNEEHQELVEHSPDKVEDHGIVTLKNVLESTKMDEGYESGTQEDQETFMNELEAVYREKGLEFKPPKFYGQPLNCLKLWRSVIKLGGYDRVTGSKLWRQVGESFHPPKTCTTVSYTFRQFYEKFQTRVSFQVGTYAVLVVDTYALLEYERHKVQIGELEFPLATVYPETPNVENETPGSGPGRARRDAAARAMQGWHAQRLVGYGEEKNSNNSQKREKSLKSIGSLKQKRPSEVEYSVKAARTEASRQLVTTVADVGPPANWVKINVLVMKDCFEVYALVPGLLREEVRVQSDPAGRVVITGQPEQPDNPWGITAFKKVINLPSRIDALRTSAVVSLHGRLLVRVPFEQSNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GHU2</accession>
    <name>A0AAD5GHU2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Subtilisin-like protease SBT1.9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">M8C21_019736</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">AA19_3_7</strain>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the peptidase S8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007772">
        <property type="protein sequence ID" value="KAI7743215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GHU2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02120">
        <property type="entry name" value="PA_subtilisin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04852">
        <property type="entry name" value="Peptidases_S8_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000006">
        <property type="entry name" value="Subtilisin-like protease SBT1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.80:FF:000003">
        <property type="entry name" value="Subtilisin-like protease SBT1.9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.80">
        <property type="entry name" value="Peptidase S8 propeptide/proteinase inhibitor I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034197">
        <property type="entry name" value="Peptidases_S8_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010259">
        <property type="entry name" value="S8pro/Inhibitor_I9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037045">
        <property type="entry name" value="S8pro/Inhibitor_I9_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045051">
        <property type="entry name" value="SBT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041469">
        <property type="entry name" value="Subtilisin-like_FN3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10795">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17766">
        <property type="entry name" value="fn3_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05922">
        <property type="entry name" value="Inhibitor_I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 10" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 10" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042226635" description="Subtilisin-like protease SBT1.9" evidence="11">
        <location>
            <begin position="22"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="domain" description="Inhibitor I9" evidence="13">
        <location>
            <begin position="24"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="12">
        <location>
            <begin position="131"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="domain" description="Subtilisin-like protease fibronectin type-III" evidence="14">
        <location>
            <begin position="649"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="9 10">
        <location>
            <position position="140"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="9 10">
        <location>
            <position position="211"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="9 10">
        <location>
            <position position="536"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF05922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF17766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAI7743215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="763" mass="82120" checksum="94D3623764836420" modified="2024-05-29" version="1">MANHITLSCVWLLSLLVLAMSHETYIVHMDLSAMPTVFSSHHSWFSATLESITTTTITSTIVTSSRLVYSYTSAINGFTAILSPYELEQVKNMPGYLYMNKDTTVELDTTHSTQFLGLNAQSGAWPVGSYGEDVIIGVVDTGVWPESESFNDAGMSSVPDRWKGECEDGDEFNSSLCNKKLIGARYFNKGLLSNRRNLTLAMNSARDTDGHGTHTSSTAAGGYVKHASYFGYGTGTAIGVAPNARVAVYKAVWEEGVFVSDILAAIDQAINDGVDVLSLSLGQDGIPLHQDPIAIATFAAVEKGIFVSASAGNNGPYLGSLHNGTPWVLTVAASTMDREFTGMLTLGNGVSVTGFALYPANSSSTHGPIYFVGACENDLDASQIEEKIVVCLDKNDTLGEQYYNVQGTNVLGAIFITNDTDVEFFMQSTYPVLLLDPESGKKVTDYMRKLNNGEAKASMKFHGTRLGTKPAPRVASYSARGPSNSCPVVLKPDITAPGSLILAAWPDSVSLATLKKDVGTGTELLYSKFNLLSGTSMSCPHASGVAALLKAAHPNWTPSAIRSAMMTTSDILDNTLQPIQDIGDYNNPATPLAMGSGHVAPNRALKPGLVYNVNTQDYVNLLCGLNYTNIQIQAITRSSTINCSNPSLDLNYPSFIAYFDGNNSKSAKVIHEFKRTVTYVGIGRSTFTAKLTGIGRLNVSVSPQKLSFQSLNEKKSYKLRIEGSRLMENEVVFGFLSWIDNKGKFVVRSPIVATSLTSTAYMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GLI7</accession>
    <name>A0AAD5GLI7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">GrpE protein homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_031546</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Probable component of the PAM complex, at least composed of SSC1 (mtHsp70), MGE1, TIM44, PAM16/TIM16, PAM17 and PAM18/TIM14. Interacts with SSQ1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the GrpE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007620">
        <property type="protein sequence ID" value="KAI7743968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GLI7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001405">
        <property type="term" value="C:PAM complex, Tim23 associated import motor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000774">
        <property type="term" value="F:adenyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00446">
        <property type="entry name" value="GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.22.10:FF:000002">
        <property type="entry name" value="GrpE protein homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.20.20:FF:000005">
        <property type="entry name" value="GrpE protein homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.20.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.22.10">
        <property type="entry name" value="Head domain of nucleotide exchange factor GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01151">
        <property type="entry name" value="GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000740">
        <property type="entry name" value="GrpE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013805">
        <property type="entry name" value="GrpE_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009012">
        <property type="entry name" value="GrpE_head"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21237:SF43">
        <property type="entry name" value="GRPE NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21237">
        <property type="entry name" value="GRPE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01025">
        <property type="entry name" value="GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00773">
        <property type="entry name" value="GRPEPROTEIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58014">
        <property type="entry name" value="Coiled-coil domain of nucleotide exchange factor GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51064">
        <property type="entry name" value="Head domain of nucleotide exchange factor GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01071">
        <property type="entry name" value="GRPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 10" id="KW-0143">Chaperone</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="28"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="71"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="54"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00063669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7743968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="247" mass="27528" checksum="3D447DD6EFBD7E0C" modified="2024-05-29" version="1" fragment="single">SYDKVIAAQVSILHNSAYNVSAFQRFGFSSSASPESNEKEKENGRTTGSSNDATENDNSDDKGEGEEDLTMEDLIKLVTEKEELLKTKQEEIEKMKDKVLRTYADMENIMDRTKREAENSKKFAIQNFAKNLLDVADNLDRASSVVKESFSKIDTSKDTAGAVPLLKTLLEGVEMTEKQLAEVLKKFGVEKYDPTNEEFDPNRHNAVFQVPDATKPPGTVAVVLKAGYMLHDRIIRPAEVGVTIKTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GMI7</accession>
    <name>A0AAD5GMI7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Survival protein SurE-like phosphatase/nucleotidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_005123</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SurE nucleotidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007182">
        <property type="protein sequence ID" value="KAI7745646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GMI7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008252">
        <property type="term" value="F:nucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1210.10">
        <property type="entry name" value="Survival protein SurE-like phosphatase/nucleotidase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030048">
        <property type="entry name" value="SurE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002828">
        <property type="entry name" value="SurE-like_Pase/nucleotidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036523">
        <property type="entry name" value="SurE-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30457">
        <property type="entry name" value="5'-NUCLEOTIDASE SURE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30457:SF0">
        <property type="entry name" value="PHOSPHATASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G01070)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01975">
        <property type="entry name" value="SurE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64167">
        <property type="entry name" value="SurE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Survival protein SurE-like phosphatase/nucleotidase" evidence="4">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="Survival protein SurE-like phosphatase/nucleotidase" evidence="4">
        <location>
            <begin position="42"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7745646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="273" mass="29452" checksum="2FF6CD125DCADB9D" modified="2024-05-29" version="1" fragment="single">MITNDDGIHAPGLQALVRVLVATDQYRVWVCAPDSDNSAARTPSDCTSLGISTALFPSVPDLVLSGINMGSNCGLHIAYSGTAAGAREAFIHGVPSVAISYEWIEGTSNINYFTLAAEACLPMISAIFVEIKNKTYPKECFLNVNLPANMLNHKHYKDRSIKELRPVPMGFKLTKQGNSIMKTGWKQVSSNSQKTTIDPSSVAMKSDAKAGSQEPLLFKMELKGSQVETDGTDFGALQEGYITVTPLGALTNADTDCHTFLKQWLPGVVECTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GND8</accession>
    <name>A0AAD5GND8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">mRNA cap-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">M8C21_003744</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">AA19_3_7</strain>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="11">EIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G. EIF4E is also known to interact with other partners. In higher plants two isoforms of EIF4F have been identified, named isoform EIF4F and isoform EIF(iso)4F. Isoform EIF4F has subunits p220 and p26, whereas isoform EIF(iso)4F has subunits p82 and p28.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the eukaryotic initiation factor 4E family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007049">
        <property type="protein sequence ID" value="KAI7746068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GND8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016281">
        <property type="term" value="C:eukaryotic translation initiation factor 4F complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000340">
        <property type="term" value="F:RNA 7-methylguanosine cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009615">
        <property type="term" value="P:response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.760.10:FF:000003">
        <property type="entry name" value="Eukaryotic translation initiation factor 4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.760.10">
        <property type="entry name" value="RNA Cap, Translation Initiation Factor Eif4e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023398">
        <property type="entry name" value="TIF_eIF4e-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001040">
        <property type="entry name" value="TIF_eIF_4E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019770">
        <property type="entry name" value="TIF_eIF_4E_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960:SF45">
        <property type="entry name" value="MRNA CAP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01652">
        <property type="entry name" value="IF4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55418">
        <property type="entry name" value="eIF4e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00813">
        <property type="entry name" value="IF4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 13" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8 13" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 13" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0810">Translation regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="23"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAI7746068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="222" mass="25354" checksum="70BA91F9C6B3F59E" modified="2024-05-29" version="1">MVEEAAKSEDLKTNNARNKQRGVRSDRQEAEGGRGDTSAGTDIIKRHPLEHSWTFWFDNPSAKSKQVAWGSSIRPIYTFSTVEEFWSLYNNIHRPSRLASGADLHCFKNRIEPKWEDPVCTGGGKWTMTFPKSKSDTCWLYTLLAMIGEQFDHGDEICGAVVNVRSRQEKIALWTKNAANETAQMSIGKQWKELLDYNDMIGFIFHEDAKKLDRGAKNKYTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GNE4</accession>
    <name>A0AAD5GNE4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Bet v I/Major latex protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_024648</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BetVI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007046">
        <property type="protein sequence ID" value="KAI7746083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GNE4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010427">
        <property type="term" value="F:abscisic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004864">
        <property type="term" value="F:protein phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009738">
        <property type="term" value="P:abscisic acid-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07816">
        <property type="entry name" value="Bet_v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.530.20:FF:000007">
        <property type="entry name" value="Major pollen allergen Bet v 1-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000916">
        <property type="entry name" value="Bet_v_I/MLP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024949">
        <property type="entry name" value="Bet_v_I_allergen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050279">
        <property type="entry name" value="Plant_def-hormone_signal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31213:SF70">
        <property type="entry name" value="MAJOR ALLERGEN PRU AR 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31213">
        <property type="entry name" value="OS08G0374000 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00407">
        <property type="entry name" value="Bet_v_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00634">
        <property type="entry name" value="BETALLERGEN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bet v I/Major latex protein" evidence="2">
        <location>
            <begin position="2"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7746083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="108" mass="11884" checksum="9C1B027C193924E6" modified="2024-05-29" version="1">MGVLAYNDERTSPVPPARIFKASILDSHILMPKLLPDAIKSIEFIEGDGGAGSIKQINFVGGFAKHQINEVDEKAFTYKYTLIEGIGISDKIEKVVEAYLLKNPDAYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GQK7</accession>
    <name>A0AAD5GQK7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Laccase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_004802</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the multicopper oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006319">
        <property type="protein sequence ID" value="KAI7749634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GQK7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001117">
        <property type="entry name" value="Cu-oxidase_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF9">
        <property type="entry name" value="LACCASE-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00394">
        <property type="entry name" value="Cu-oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Plastocyanin-like" evidence="3">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="4">
        <location>
            <begin position="184"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7749634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="325" mass="35933" checksum="D5091546B1732E6A" modified="2024-05-29" version="1" fragment="single">FFFKIANHNLTVVAVDAAYTNPLQTDVVVVGPGQTTDVLLTANQSPGLYYMVANPYANAVGVPFNGNTTTGILSYPNATQTTPIPILPVLPVFNDTPTAFTFSSNLTALVTSPFWSPVPQTVDEKMFITMGLGLSTCGSNQTCGGIFGQRMAASMNNNSFVLPTRMSLLEAFFRNVSGVYTTDFPDGPPMVFDYTNVNNSFNQSLLMTPKLTNLKKIKFNSTVEIVLQNTALIGIENHPMHLHGFNFYNETHWECPLAAGLSSDSEQIILVCGLFTAIWKYICHGVWAQLSWWRTEGHRNLHCRHRLPTFLSARSRSICIHLLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GR77</accession>
    <name>A0AAD5GR77_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein ECERIFERUM 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_021928</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RNase PH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006341">
        <property type="protein sequence ID" value="KAI7749561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GR77"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035925">
        <property type="term" value="F:mRNA 3'-UTR AU-rich region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000467">
        <property type="term" value="P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071028">
        <property type="term" value="P:nuclear mRNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071035">
        <property type="term" value="P:nuclear polyadenylation-dependent rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071038">
        <property type="term" value="P:TRAMP-dependent tRNA surveillance pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034473">
        <property type="term" value="P:U1 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034476">
        <property type="term" value="P:U5 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11368">
        <property type="entry name" value="RNase_PH_RRP45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.70:FF:000007">
        <property type="entry name" value="Exosome complex component RRP45B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="entry name" value="GHMP Kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001247">
        <property type="entry name" value="ExoRNase_PH_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015847">
        <property type="entry name" value="ExoRNase_PH_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036345">
        <property type="entry name" value="ExoRNase_PH_dom2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050590">
        <property type="entry name" value="Exosome_comp_Rrp42_subfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033100">
        <property type="entry name" value="Rrp45"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11097:SF14">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT RRP45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11097">
        <property type="entry name" value="EXOSOME COMPLEX EXONUCLEASE RIBOSOMAL RNA PROCESSING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01138">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03725">
        <property type="entry name" value="RNase_PH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55666">
        <property type="entry name" value="Ribonuclease PH domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="9">
        <location>
            <begin position="37"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="10">
        <location>
            <begin position="197"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="313"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="364"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="313"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="382"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="402"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="418"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00079975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7749561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="429" mass="46901" checksum="4954CF0A87DA3845" modified="2024-05-29" version="1">MEQRLANSLIMTVNEKKFIESALLSDIRVDGRRPFDYRNLTIRFGSEDGSAEVQLGQTQVIGFVTGQLTQPYRDRPNEGSFAIFTEFSPMADPSFEPGRPGETAIELGRIVDRGLRESRAIDTESLCVIAGKLVWSIRIDLHIIDNGGNLVDAANIAALAALSTFRRPECTLGGEDGQELIVHPPDVREPLPLILHHFPVAVTFGFIGKENTVLIDPTHYEEAVMGGSMTATLNTNGDVCAIQKAGGDGVLQTVIMQCLRIASVKAGDITSKIKNAVESYNTQRSLRKIKRHNPDQQGSSDVARHHMERLTVGSDQTGIASQFNEHDKTNKRNSTLKGFSGGPASWDPYSKGVDGDALRTTLASRVIPPLDKQKEESSQVNPETKSSPKSSNTNSFASTHESGLEKKQSKTLKDAVKPKHKRRNKSNAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GTA6</accession>
    <name>A0AAD5GTA6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_010563</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005857">
        <property type="protein sequence ID" value="KAI7751676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GTA6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7751676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="39" mass="4431" checksum="0A7AB04A0F2DB13E" modified="2024-05-29" version="1" fragment="single">MKLEIHGENKRNSRNGASRKLAAALQGFPRFLLFLIYVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GY37</accession>
    <name>A0AAD5GY37_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ethanolamine-phosphate cytidylyltransferase</fullName>
            <ecNumber evidence="10">2.7.7.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">CTP:phosphoethanolamine cytidylyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_010962</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="9">Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from ethanolamine: step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytidylyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.14" evidence="10"/>
    <dbReference type="EMBL" id="JAMZMK010000416">
        <property type="protein sequence ID" value="KAI7756386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GY37"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004306">
        <property type="term" value="F:ethanolamine-phosphate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006646">
        <property type="term" value="P:phosphatidylethanolamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02174">
        <property type="entry name" value="CCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02173">
        <property type="entry name" value="ECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041723">
        <property type="entry name" value="CCT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044608">
        <property type="entry name" value="Ect1/PCYT2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45780">
        <property type="entry name" value="ETHANOLAMINE-PHOSPHATE CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45780:SF5">
        <property type="entry name" value="ETHANOLAMINE-PHOSPHATE CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="7" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="20"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="domain" description="Cytidyltransferase-like" evidence="13">
        <location>
            <begin position="69"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="Cytidyltransferase-like" evidence="13">
        <location>
            <begin position="265"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7756386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="428" mass="48187" checksum="991E935E7B6CEFBE" modified="2024-05-29" version="1">MEFESNDSNWDGVYYYPQLFGGVMLTAAVLGLSTSYFGGTVGLPTLPYMLPYLKNLQKKKREKRKIRVYMDGCFDLMHYGHANALRQAKALGDELVVGLVSDEEIVANKGPPVLSMEERLALVSGLKWVDEVIADAPYAITEEFMSRLFNEHKIDYIIHGDDPCLLPDGSDAYALAKKVGRYKQIKRTEGVSSTDIVGRILASMEDKKVCQVPQESSWNDEPNEMNKNVESLIKSKPASNFLPTSRRIVQFSNAKGPGPNARVVYIDGAFDLFHAGHVEILKRARQLGDFLLVGIHTDEIVSEQRGPHFPLMHLHERSLSVLACRYVDEVIIGAPWKITKDMITTFNIKLVVHGTVCESKSSVNSQPDPYSVPKNMGMFRMIESPKDITTTSVAQRIKANHEIYEKRNAKKEASEKKFYEERKYISGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GYX2</accession>
    <name>A0AAD5GYX2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GDSL esterase/lipase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_025736</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000170">
        <property type="protein sequence ID" value="KAI7757166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GYX2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46020:SF32">
        <property type="entry name" value="GDSL ESTERASE_LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46020">
        <property type="entry name" value="OSJNBB0059K02.9 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7757166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="360" mass="39657" checksum="3875A53A08B7C9F3" modified="2024-05-29" version="1">MELQGILSYIFFLMCLFLITGNFTSEFTVAGNIHHQIHKHGRFRRPTKLFVFGDSYADTGNNPKAFASSWKPPYGVTFPGKPAGRYSDGRVFTDYLARFMGIRSPLPYKFRKYAPGKLRGGLNFACGGTGVFDTGNLQPNLTTQIGFLQGLIKERVYTKRDLKASLALVTISGNDYGTYTSTGGSEQGLPTFISQVVNQLAKDLKLLHDTGVRRVLVTTLQPIGCLPTVTVSSSYQQCNVSQNSAAGFHNQLLQHAVAKLNNNTKDSSFLILDIFSAFNTVLKSKRDLTGNSKFDTPLKPCCIATSSDANCGSLDENGKKLYTVCKRPASTFFWDTVHPTQAGWHAVYITLRSSLNRINA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5H0L2</accession>
    <name>A0AAD5H0L2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FAD-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_009637</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the 3-hydroxybenzoate 6-hydroxylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000010">
        <property type="protein sequence ID" value="KAI7758278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5H0L2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044560">
        <property type="entry name" value="MOase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45934">
        <property type="entry name" value="FAD/NAD(P)-BINDING OXIDOREDUCTASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45934:SF28">
        <property type="entry name" value="OS03G0153100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding" evidence="4">
        <location>
            <begin position="9"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7758278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="414" mass="45907" checksum="B1A4EAE7A7E4C6C4" modified="2024-05-29" version="1">MKVHNHEDIDIVIVGAGLAGLTTALALHRLGLTALVLESSESLRVTGFALTLWTNAWRALDVVGIGDSLRQMCIQIKGFKMVSPDTGLFTSGQALDKDEKFKGFESRCVRRKDLLETIVNELPPGTIRYSSKVVTIDGLDRFKMVHLADGLILKTKVLIGCDGINSVVARWLALPAPVSVGRSAIRGLVKFPNGSGFDPMSHLNFGGGTRFGFRPVDDKTVYWFCTFTHSQVPSYDKELQENPMKMKQFVLGLINKMPREAQEVVEKTSLSNISYSTLKLRLPWDILFGNIVKENVCVAGDALHPMTPDINQGGCSALEDAIVLARCLGEAFLKKSSEKEDEFDRIEKGLLKYGRERRWRSVSLISVAYCVGFIQQSKGKVMSFLRKVWLSNYTSTALLKMSNYDCGDLVSKSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BJY3</accession>
    <name>A0AAD5BJY3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_012848</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012185">
        <property type="protein sequence ID" value="KAI7724577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BJY3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7724577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="60" mass="6511" checksum="FB5B9FFBC5E13507" modified="2024-05-29" version="1">MYADLISRISAPIPTPNTSYNELELPVQNDLIVSTVVGRSAVRLGELNNKEEDAAIAIDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BKC0</accession>
    <name>A0AAD5BKC0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">M8C21_005753</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">AA19_3_7</strain>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Required for vesicular transport from the ER to the Golgi complex. Functions as a SNARE associated with ER-derived vesicles.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the BET1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012154">
        <property type="protein sequence ID" value="KAI7724807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BKC0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15841">
        <property type="entry name" value="SNARE_Qc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000056">
        <property type="entry name" value="Bet1-like protein At4g14600"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12791">
        <property type="entry name" value="GOLGI SNARE BET1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="15">
        <location>
            <begin position="40"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="43"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00060029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAI7724807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="165" mass="18555" checksum="F6A2CE232D6A58E5" modified="2024-05-29" version="1">MASSSNRGGDGYYGAAPYRSREGLSTRQGTGSDEIQLRIDPMHADLDDEISGLRKQVRQLKNVATEIESEAKFQNEFINQLQMTLIKAQAGVKNNMRKLNKSIIQSGSSSYLSITKPELAEKLVWAQSKGRVGWLYELKPWLCGLVKFFDKMGTMVKESWRSGVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BNH7</accession>
    <name>A0AAD5BNH7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cyclin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_014185</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclin family. Cyclin AB subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011624">
        <property type="protein sequence ID" value="KAI7726631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BNH7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd20506">
        <property type="entry name" value="CYCLIN_AtCycA-like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000013">
        <property type="entry name" value="Cyclin A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000167">
        <property type="entry name" value="Mitotic cyclin 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039361">
        <property type="entry name" value="Cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004367">
        <property type="entry name" value="Cyclin_C-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048258">
        <property type="entry name" value="Cyclins_cyclin-box"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10177">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02984">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01332">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00292">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="3 5" id="KW-0195">Cyclin</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="6">
        <location>
            <begin position="133"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin C-terminal" evidence="7">
        <location>
            <begin position="226"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="6">
        <location>
            <begin position="230"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7726631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="363" mass="41589" checksum="7DB5C708ABD0AD6E" modified="2024-05-29" version="1">MGDQENNGEVRVTRLAKKRAMEAIGCQLQPVNKRKRVVLGELSNNQVVGCDVVDIKTCGSKKVKNKSVKSVIERDLDVFDGTGSDPQMCEAYVNDIHEYLSSMEMEVKRRPVADYIEKVQKDVTVNMRSVLIDWLVEVAEEYKLLPDTLYLTVSYIDRYLSVNVLNRQRLQLLGVSSMLIASKYEEITPPHTEDFCYITDNTYTKQEVVKMEADVLKTLKFEMGNPTVKTFVRRFTRLAQEDYDTPNLQLEFLSYYLAELSLLEYSCIKFLPSLVAASVTFLSRFMLKPESHPWNLPLEELSGYKPSDLKECVEILHDLQSSRRAGNLVAIREKYNQHKFKCVSGLSSPSVIPTSYFEDIKTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BNL2</accession>
    <name>A0AAD5BNL2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">alpha,alpha-trehalose-phosphate synthase (UDP-forming)</fullName>
            <ecNumber evidence="3">2.4.1.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_018783</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">In the C-terminal section; belongs to the trehalose phosphatase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">In the N-terminal section; belongs to the glycosyltransferase 20 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.15" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010011678">
        <property type="protein sequence ID" value="KAI7726404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BNL2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003825">
        <property type="term" value="F:alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004805">
        <property type="term" value="F:trehalose-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005992">
        <property type="term" value="P:trehalose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03788">
        <property type="entry name" value="GT20_TPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01627">
        <property type="entry name" value="HAD_TPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000100">
        <property type="entry name" value="Alpha,alpha-trehalose-phosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000046">
        <property type="entry name" value="alpha,alpha-trehalose-phosphate synthase [UDP-forming] 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000039">
        <property type="entry name" value="alpha,alpha-trehalose-phosphate synthase [UDP-forming] 1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001830">
        <property type="entry name" value="Glyco_trans_20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012766">
        <property type="entry name" value="Trehalose_OtsA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003337">
        <property type="entry name" value="Trehalose_PPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011071">
        <property type="entry name" value="PRK14501.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02400">
        <property type="entry name" value="trehalose_OtsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788:SF130">
        <property type="entry name" value="ALPHA,ALPHA-TREHALOSE-PHOSPHATE SYNTHASE [UDP-FORMING] 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788">
        <property type="entry name" value="TREHALOSE-6-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00982">
        <property type="entry name" value="Glyco_transf_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02358">
        <property type="entry name" value="Trehalose_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="806"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="7"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="811"/>
            <end position="824"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7726404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="914" mass="102908" checksum="B46BA558FDA5956D" modified="2024-05-29" version="1">MPGNKYNNNNGNSSSVPSSRIERLLRERELRKNSRASYANEPTTNDGIEFSDNLLEQYLEGASAARDGWEKPDGGLFTQRLLVVANRLPVSAVRRGEESWSLEVSAGGLVSALLGVKEVEAKWIGWAGVNVPDEPGQRALTKALAEKRCIPVFLDEELVHQYYNGYCNNILWPLFHYLGLPQEDRLATTRSFQSQFAAYKKANQMFADVVNEHYEEGDVVWCHDYHLMFLPKCLKDHNRNMKVGWFLHTPFPSSEIHRTLPSRSELLRAVLAADLVGFHTYDYARHFVSACTRILGLEGTPEGVEDQGRLTRVAAFPIGIDSDRFIRALENPLVQEHMKELKERFSGRKVMLGVDRLDMIKGIPQKILAFEKFLEENPYWRDKVVLLQIAVPTRTDVPEYQKLTSQVHEIVGRINGRFGTLTTVPIHHLDRSLDFQALCALYAVTDIAIVTSLRDGMNLVSYEFVACQDDKRGVLILSEFAGAAQSLGAGAILVNPWNITEVAASIGQALNMSAEEREKRHRHNFLHVTTHTAQEWAETFVSELNDTVVEAQQRIRQVPPPLPVEEAIERYLQSSNRLIILGFSATLTEPVDTPDRRGGDQIREMDLKLHPELKESLTKLCDDPHTTVVVLSGSDRTVLDDNFGEFNMWLAAENGMFLRSTKGTWMTTMPEHSNMEWVDSVKHVFEYFTERTPRSHFELRETSLVWNYKYADVEFGKLQARDMLQHLWTGPISNASVDVVQGSRSVEVRAAGVTKGITIVRILGEIVHSKSISSPIDYVLCIGHFLGKDEDIYTFFEPELPYNGMGIPRPKLNDPAKGPGDKRPGKSGSKSSQKARAPQSPDNKRTTNTNRNNGFENGGKRSSVSADKVSWNVLDLKADNYFSCAVGRTRTNARFVIPSSDEVVTFLKELTQAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BP77</accession>
    <name>A0AAD5BP77_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">glucan endo-1,3-beta-D-glucosidase</fullName>
            <ecNumber evidence="4">3.2.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">M8C21_002255</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">AA19_3_7</strain>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of (1-&gt;3)-beta-D-glucosidic linkages in (1-&gt;3)-beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.39"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <topology evidence="2">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the glycosyl hydrolase 17 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.39" evidence="4"/>
    <dbReference type="EMBL" id="JAMZMK010011592">
        <property type="protein sequence ID" value="KAI7726719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BP77"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009506">
        <property type="term" value="C:plasmodesma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042973">
        <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000002">
        <property type="entry name" value="Glucan endo-1,3-beta-glucosidase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1040:FF:000001">
        <property type="entry name" value="Glucan endo-1,3-beta-glucosidase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000490">
        <property type="entry name" value="Glyco_hydro_17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044965">
        <property type="entry name" value="Glyco_hydro_17_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012946">
        <property type="entry name" value="X8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32227">
        <property type="entry name" value="GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00332">
        <property type="entry name" value="Glyco_hydro_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07983">
        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00768">
        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0611">Plant defense</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 16" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042044722" description="glucan endo-1,3-beta-D-glucosidase" evidence="16">
        <location>
            <begin position="24"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="domain" description="X8" evidence="17">
        <location>
            <begin position="365"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAI7726719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="457" mass="50004" checksum="6D3813FA72399E4A" modified="2024-05-29" version="1">MSSGFWLVFATSLLLMLLGTCNGSKVGICYGRNADDLPTPDKAVDLIKNQNISYVRIYDSNIQVLKAFANTGIELMVGIPNSDLLPFSQFQSNADTWLKNSILPYYPATKITYITVGAEVTEARDNSSSMVVPAMTNVYNALRKAGLHRRIKVSTTHSLGVLSRSYPPSAGSFNSKHAGFLKPLLDFLAENQSPFLVNVYPYYAYRDSSNNVSLDYALFESSSEVIDPNTGLLYMNLFDAQIDSIYFALAGLNYKTIKIKVTETGWPSKGSPKETAATPENAQTYNTNLIRHVINNTGTPARPGEELDVYIFSLFNENRKPGLDSERNWGLYYPDQTSVYNVDFTGKGTVDVNNGGNLTTVNGTSWCIASPNASEVDLRNGLDWACGSGNVDCSPIQPSQPCFQPDSLVSHASYAFNSYYQQNGATDIACSFGGAGVKTNKNPSYDNCLYMTAGDKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BPI2</accession>
    <name>A0AAD5BPI2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_004219</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011408">
        <property type="protein sequence ID" value="KAI7727231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BPI2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38371">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38371:SF1">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="246"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="29"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="80"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7727231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="350" mass="39060" checksum="12A209521B770CEA" modified="2024-05-29" version="1">MADFEPPSFSLGLDYNLFDSEPQTPPVPQHTHPSSSSSNRFSLPFEDNNDDFQTLVVDDSEPEYPDSDPKLNRRRRSLTVGATSSSSPLLHSRHTDTDQHLHTQHHVVCTSLNGLEPSCDNAVFPKLAASPVRGFCLTHSDSDACDRVVSRKCNGSDSNSNHGKFDDLPQHVQRKQNVSIAWGDFPKAYRYFFHDDPRIQELVRSRLPNFFPLGNMLNTGLEQPGTSKIDYMGQFGVGESSRQAARTIKENKSSTSRKNSGKLKTEEMSQGWVNPKLGVDKVTTKGAVKRSVHTVRQSAGHWLTGSDGKKVYVGKSGQELTGRVAYMQYKKESGFGFRKVKRKSVAKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BPP7</accession>
    <name>A0AAD5BPP7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pollen Ole e 1 allergen and extensin family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_004225</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011408">
        <property type="protein sequence ID" value="KAI7727237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BPP7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38400">
        <property type="entry name" value="OS02G0317800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01190">
        <property type="entry name" value="Pollen_Ole_e_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042099926" description="Pollen Ole e 1 allergen and extensin family protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7727237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="148" mass="16318" checksum="340A9463B9A05660" modified="2024-05-29" version="1">MEHKSTMVNLFITLLIFPFITTCTVKAWTGEIHGRVVCDVCADGSVGPEDHVLEGAEVAVLCITKSGEVLNYQAFTNSRGIYTVAETMPESERWDACLARPISSFHEHCTHLGDGFSGVKFSYNHVSGYTHAVKPFVYRPVSIPIYCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BQE4</accession>
    <name>A0AAD5BQE4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">MYB-CC type transcription factor LHEQLE-containing domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_014190</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011624">
        <property type="protein sequence ID" value="KAI7726636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BQE4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000118">
        <property type="term" value="C:histone deacetylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.960.30:FF:000001">
        <property type="entry name" value="F-box-like/WD repeat-containing protein TBL1XR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000218">
        <property type="entry name" value="WD40 repeat-containing protein HOS15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.960.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045183">
        <property type="entry name" value="Ebi-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025756">
        <property type="entry name" value="Myb_CC_LHEQLE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22846:SF2">
        <property type="entry name" value="F-BOX-LIKE_WD REPEAT-CONTAINING PROTEIN EBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22846">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08513">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14379">
        <property type="entry name" value="Myb_CC_LHEQLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00667">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2 7" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="263"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="369"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="411"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="462"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="domain" description="MYB-CC type transcription factor LHEQLE-containing" evidence="9">
        <location>
            <begin position="588"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="100"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="197"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="113"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF14379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7726636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="714" mass="81881" checksum="DC456ED13A275F3B" modified="2024-05-29" version="1" fragment="single">MINMNSHDLNYLVYRYLHESGFTHTAFTLGYEAALNKSTIDGNVVPPGALVTFVQKGIQYLELEANLTCNDSDIDEDFSFIQPIDLITKNVYELQQMIKEKKQNLPKTKHRERNRDNREHEQEHSREKDREHSREKEKEHAKGREKEIEQEHAREREKERQHVREREKKMEIEIYREKEQEKEKHIRDKERELEKEKIEREKDKAKQKEKKNEDLADVNRDEDKIRVQHEENVTPEVGADPMEIDNNSAPMSALIPNSDVTVLKGHTSEVFVCAWSPTESLLASGSGDSTARIWTIRDGPCRSDSQYGPSNGEGTLLATGSYDGQARIWSKDAPTLDVDWRNNVSFATCSTDNMIYVCKVGDTRPVKTFAGHQGEVNAIKWDPTGTLLASCSDDSTAKIWSLKQDTCLHDFKEHTKEIYTIRWSPTGPGTNNPNQPLVLASASFDSSIKLWDVETGKVLHSLVAHGDPVYSVAFSPNGEYLASGSLDKCMHIWSVKEARVVKTYEGSGGIFEVCWNKQGDKIGACFSNNVVCILDFRMRLDAFSFRAAKELRRWDYGKRKRDHSKGEDGNWEKIVFADAATYMKINFQVTEALRTQMEIQRRFHEQLEAQRKLQFRIETQGNYLKAILENAHKADNTVGRQNIGEVGLEAPNLQLSEWISPVSATYLRDDGMKHLSNQPTFFSTEGMDVEMMDVNYLSSGNNEPLKLKDRFFFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BR92</accession>
    <name>A0AAD5BR92_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_031883</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011474">
        <property type="protein sequence ID" value="KAI7726998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BR92"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052370">
        <property type="entry name" value="Meta-cleavage_hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43139:SF22">
        <property type="entry name" value="AB HYDROLASE-1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43139">
        <property type="entry name" value="SI:DKEY-122A22.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042214040" description="AB hydrolase-1 domain-containing protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="AB hydrolase-1" evidence="2">
        <location>
            <begin position="58"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7726998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="309" mass="35169" checksum="074346B3E3B16BBC" modified="2024-05-29" version="1">MGNTIVLLTPLLHGLVKLAGLTPQTIEIEPGTTMNIWVPKETVTKHDQNIVYVPPTKPAVLLLHSFAMDGIFTWFLQVFALTREYAVYVPDFLFFGGSITDRNERTANFQAEFVAKGLGKLNVEKVTLVGLSYGGMVGFKMAKMYPNLVKSMVVSGTVVELTESISLESYKRLGLMRWSDLLMPKTVEGLKMMFSIGFHKVPWVPDFIYRDLLETMFSNRKERNELLEALVVSDEDATSYTNYPQEIHMVWGDDDKIFDLDLATTMKTRLGDKASLEWIKDAGHLVPLEQPFKYNKRLKCILEHVTKYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BRX7</accession>
    <name>A0AAD5BRX7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Heparanase-like protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_001069</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Endoglycosidase which is a cell surface and extracellular matrix-degrading enzyme. Cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Lysosome membrane</location>
            <topology evidence="9">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 79 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011193">
        <property type="protein sequence ID" value="KAI7728551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BRX7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009505">
        <property type="term" value="C:plant-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004566">
        <property type="term" value="F:beta-glucuronidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000023">
        <property type="entry name" value="heparanase-like protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005199">
        <property type="entry name" value="Glyco_hydro_79"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14363:SF17">
        <property type="entry name" value="HEPARANASE-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14363">
        <property type="entry name" value="HEPARANASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03662">
        <property type="entry name" value="Glyco_hydro_79n"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0458">Lysosome</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042223805" description="Heparanase-like protein 3" evidence="11">
        <location>
            <begin position="27"/>
            <end position="545"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00055929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7728551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="545" mass="60809" checksum="8F3218A82410A72C" modified="2024-05-29" version="1">MGSFILQMGVCFYVLLFSFSCNLVYSQIQTRKDVLQGKIFINGKTSIAKIDSDFICATMDWWPPEKCDYGTCSWGHASLLNVDLNNKIFQNAIKAFSTLKIRLGGSLQDKVVYQTENDPKPCTQFSRNTSEMFGFTQGCLPLSRWDQLNTFFKETGAEIIFGLNALAGKKILANGSAVGPWDPANAESLIRYTVKKNYPMYAWELGNELSGSGVGTRITASQYAFDTKALKNIVQKSYEGIEPKPRIIAPGGFFDANWFKEFIDKTPKTLDIITHHIYNLGPGVDQHLVEKILDPSYLDGEADTFKQLENILKASGTSVSAWVGEAGGAYNSGHNLVTNAFVFSFWYLDQLGMSSVYDTKTYCRQSLIGGNYGLLNTTTFVPNPDYYSALLWHRLMGQKVLSVNFNGTKKIRSYAHCAKESQGITLLLINLDSKTTVDVSLSLDSTWSLHKLRSRAHYHHHKANKPRHSKISGTNTREEYHLTAKDGDLHSQVMMLNGKELSVNSNGEIPELVPMYVDSSEPISVAPFSIVFAHIPYLTLDACNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BTB6</accession>
    <name>A0AAD5BTB6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_002173</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011069">
        <property type="protein sequence ID" value="KAI7729110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BTB6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004306">
        <property type="term" value="F:ethanolamine-phosphate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006646">
        <property type="term" value="P:phosphatidylethanolamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044608">
        <property type="entry name" value="Ect1/PCYT2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45780">
        <property type="entry name" value="ETHANOLAMINE-PHOSPHATE CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45780:SF11">
        <property type="entry name" value="ETHANOLAMINE-PHOSPHATE CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7729110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="162" mass="18330" checksum="8C219A6B98CCC32B" modified="2024-05-29" version="1">MHLHERSLSVLACRYVDDVIIGAPWEISKDMITTFNISLVGHGTVSEVNYTPDAVVDPYKVPKSMGIFRTLESPKNITTTFVAERIKANHEIYQVLAMDIYNRYKKNISCILEKRDRADPKGSKSEEYDDDNNKNNVLLMGQRAQAIVAGRTEKGPSYMDVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BV39</accession>
    <name>A0AAD5BV39_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Katanin p60 ATPase-containing subunit A1</fullName>
            <shortName evidence="8">Katanin p60 subunit A1</shortName>
            <ecNumber evidence="8">5.6.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">p60 katanin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">KATNA1</name>
        <name evidence="11" type="ORF">M8C21_029427</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Severs microtubules in an ATP-dependent manner. Microtubule severing may promote rapid reorganization of cellular microtubule arrays.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>n ATP + n H2O + a microtubule = n ADP + n phosphate + (n+1) alpha/beta tubulin heterodimers.</text>
            <dbReference type="EC" id="5.6.1.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cytoplasm</location>
            <location evidence="1 8">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the AAA ATPase family. Katanin p60 subunit A1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.1.1" evidence="8"/>
    <dbReference type="EMBL" id="JAMZMK010010909">
        <property type="protein sequence ID" value="KAI7729824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BV39"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008568">
        <property type="term" value="F:microtubule severing ATPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051013">
        <property type="term" value="P:microtubule severing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000055">
        <property type="entry name" value="Katanin p60 ATPase-containing subunit A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000159">
        <property type="entry name" value="Katanin p60 ATPase-containing subunit A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03023">
        <property type="entry name" value="Katanin_p60_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028596">
        <property type="entry name" value="KATNA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050304">
        <property type="entry name" value="MT-severing_AAA_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074">
        <property type="entry name" value="AAA DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074:SF155">
        <property type="entry name" value="KATANIN P60 ATPASE-CONTAINING SUBUNIT A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7 8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3 8" id="KW-0493">Microtubule</keyword>
    <keyword evidence="4 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="10">
        <location>
            <begin position="113"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="121"/>
            <end position="128"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7729824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="365" mass="40725" checksum="86433EA8CA12E409" modified="2024-05-29" version="1">MVSPKICYLVNLAAMRHLLAIRPLWYFGSGFRFYCPPAYDYQNGDAEEGKSKRGQYEGPDADLAATLERDVLDAAPGVRFDDVAGLSEAKRLLEEAVVLPLLMPDYFQGIRRPWKGVLMFGPPGTGKTLLAKAVATECGTTFFNVSSASLASKWRGESERMVRCLFDLARSYAPSTIFIDEIDSLCTSRGASGEHESSRRVKSEVLVQVDGVNNTGTNEDGSRKIVMVLAATNFPWDIDEALRRRLEKRIYIPLPNFESRRELIKINLRTVEVAADVDIDEVARRTEGYSGDDLTNVCRDASLNGMRRKIAGKTREEIKNMSKEDISKDPIAMCDFEEAITKVQPSVSAADIERHEKWFAEFGSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BWU8</accession>
    <name>A0AAD5BWU8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_030808</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DnaX/STICHEL family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010513">
        <property type="protein sequence ID" value="KAI7731208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BWU8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009360">
        <property type="term" value="C:DNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005663">
        <property type="term" value="C:DNA replication factor C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003689">
        <property type="term" value="F:DNA clamp loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18137">
        <property type="entry name" value="HLD_clamp_pol_III_gamma_tau"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000013">
        <property type="entry name" value="DNA polymerase III subunit gamma/tau"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012763">
        <property type="entry name" value="DNA_pol_III_sug/sutau_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050238">
        <property type="entry name" value="DNA_Rep/Repair_Clamp_Loader"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054506">
        <property type="entry name" value="DnaA_N-like_STI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045085">
        <property type="entry name" value="HLD_clamp_pol_III_gamma_tau"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02397">
        <property type="entry name" value="dnaX_nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669:SF46">
        <property type="entry name" value="PROTEIN STICHEL-LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669">
        <property type="entry name" value="REPLICATION FACTOR C / DNA POLYMERASE III GAMMA-TAU SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13177">
        <property type="entry name" value="DNA_pol3_delta2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23007">
        <property type="entry name" value="DnaA_N-like_STI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22608">
        <property type="entry name" value="DNAX_ATPase_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="post-AAA+ oligomerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="9">
        <location>
            <begin position="405"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="60"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="195"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="279"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="351"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="714"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="660"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="76"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="94"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="110"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="203"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="301"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="729"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="928"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7731208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="928" mass="102369" checksum="3017B0E56317AF6E" modified="2024-05-29" version="1" fragment="single">MTRVVRDRILKDANGDISDHLRNHIHLTNCIHLKNHMHKNSPITTDRSLIRDLVVLQRSRSLRDPSMSPPPFQSPSGGGGGGGGGGGDGGARRSVGLSGNSSSNVGTGDVIHRRIKKEESSRRSFNDDSPLDAANLARDIYPGSSDSKGKQKGRKSIKSKQDDHVKTLLQQLNDGPIDSDNVASSHNRFHGGVEVESSLHSHGVSRAKRRKFKGGRRASRAPKNEVSMASNFVPHVDFDGQQNVSGAPKNGCGIPFNWSRIHDRGKSFLDIAGKSLSCGLSDSRSKRGGQRDSGQIPVLSDDSTSSTNGESLPLLLDGSQGSRENPAAWVHDYSGELGIYADNLLNGGTDSDLASEARSGHQKRSHDNTRHQNLTQKYTPRTFRDLVGQNLAVQALSNAIAKKKVGLLYVFYGPHGTGKTSCARIFARALTCQSLDHPKPCGYCNSCVAHDMGKNRSVREVGPMNNLGYKRIMELIENMIVSHIPSQYRVFIIDDCDSLPPNCWSAISKVIDRAPRRMVFLLVNSSLDVLPHIIISRCQKFFFPKLKDADIIYTLQWIATKEDFEIDKDALKLIASRSDGSLRDAEMTLEQLSLLGQRISVSLVQELVGLISDDKLVDLLDLALSADTVNTVKHLREIMESGVEPLALMSQLATVITDILAVSKDDMERLRLALKTLSEAEKQLRTSNDKLTWLTAALLQLAPDQQYVLPTSSVATSTHHSPMGLSNGGRRDGPRKSNFEHGETTGIQRNILKNVGNSTKMKERLYQQEIEEIWFEVLENIPINSIKEFLYHEGKLTSVSFGAAPTVQLNFSSHVTKSKAEKFKTHILKAFEHVLGSSVTIEIRKATTSHNNSNIGFQRRLGEQSQSLSLVKGKVSLANVIQQAESHRNGWSTRKAVSIAEKLEQENLRMEPRSRSLLCWKASRVARR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BZJ9</accession>
    <name>A0AAD5BZJ9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_002577</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010483">
        <property type="protein sequence ID" value="KAI7731301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BZJ9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14702">
        <property type="entry name" value="bZIP_plant_GBF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045314">
        <property type="entry name" value="bZIP_plant_GBF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="7">
        <location>
            <begin position="18"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="8"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7731301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="62" mass="6946" checksum="EE6FD3FE55C84E67" modified="2024-05-29" version="1">MPSLGSDGDPKYANLDERESARPSRAKKQQKLDELLAEISRLQNENNTIMKKIDGALTSCIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BZV7</accession>
    <name>A0AAD5BZV7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">WAT1-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_028718</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the drug/metabolite transporter (DMT) superfamily. Plant drug/metabolite exporter (P-DME) (TC 2.A.7.4) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010161">
        <property type="protein sequence ID" value="KAI7732781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BZV7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030184">
        <property type="entry name" value="WAT1-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31218">
        <property type="entry name" value="WAT1-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="16"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="45"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="78"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="106"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="189"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="221"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="253"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="285"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="311"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="7">
        <location>
            <begin position="17"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="7">
        <location>
            <begin position="191"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7732781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="358" mass="39856" checksum="7DD0179B8D32E20E" modified="2024-05-29" version="1">MMNSPKLVMAYENNKPYIAMFIVQAIYAGMALLSKASISSGMNPFIFVVYRQAFAFLSLAPFAYFFERKNVAHLSYTLTWKIFFSSLIGITICMNMYYHALGHTSATFAAATTNLIPAITFVIALALRIERMYIRELHGWAKLIGSIVSVSGALVFAFVKGPTLNFMTWYTPSTEVGSITNQFSSKGEFIKGPFMILCANVLWSCWGIIQAPIMKEYPAKLSLITLQCFFSLTQATVAAIAMERNLSAWKLGWDVNLLSVAYCGVIVTALTYWLQLWSIEKRGPVFTSMFTPLALIITAIVSMFVWKEPLYVGSVCGAVLLAGGLYCVLWGKSKEMKGTITEEKPEVEITLESIKTQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C1U7</accession>
    <name>A0AAD5C1U7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF1421 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_006467</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009993">
        <property type="protein sequence ID" value="KAI7733440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C1U7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010820">
        <property type="entry name" value="DUF1421"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31805:SF16">
        <property type="entry name" value="FORMIN-LIKE PROTEIN (DUF1421)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31805">
        <property type="entry name" value="RECEPTOR-LIKE KINASE, PUTATIVE (DUF1421)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07223">
        <property type="entry name" value="DUF1421"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF1421" evidence="2">
        <location>
            <begin position="407"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="162"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="177"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="201"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="216"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="278"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="317"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7733440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="458" mass="50889" checksum="686AD0D33A0786AC" modified="2024-05-29" version="1">MASGSSKGFDFASDDNILCSYQDYTHQEQSNGSHLDSAISSNPTKQEFNKNRMARASVFPINDCDNQEVILMAVERSMKKHTDNVMRFLEGLSSRLSQLELYCYNLDKSIGQMRSDLTRDHEESDAKLKFLEKHLQEVHRSVQILRDKQELAETQKELAKLKIARKESSSSHNSQQNDERSSTPDTRKNETHGQQLALVPAPQPAPVPAPQPPMTQPQAYYLPPGSTQYPPQPPQVQSVPQYQQQWTPQVQPQPQPQPMMQPVSITSSPQIYPQYLPTKPPATPSPAPLPNSTPMQMTYSAMPPPGSNQPMTYSYGPPAQTAQPQLPPQAQQQQQHLKTGYGAQPGEGYVAGGPPNTYMVYDNASGRVQHPNQGGQYYPPNQQQGSVISGGGMMPRPSQQFMRGHPYSEVVDKLVSMGYRSDHVMSVIQRMDEAGQTIDFNSVLDQLNGHSSGRGWSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C407</accession>
    <name>A0AAD5C407_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">phosphatidate phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_018662</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the lipin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.4" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010009929">
        <property type="protein sequence ID" value="KAI7733684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C407"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008195">
        <property type="term" value="F:phosphatidate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026058">
        <property type="entry name" value="LIPIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031703">
        <property type="entry name" value="Lipin_mid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007651">
        <property type="entry name" value="Lipin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013209">
        <property type="entry name" value="LNS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031315">
        <property type="entry name" value="LNS2/PITP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12181">
        <property type="entry name" value="LIPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12181:SF59">
        <property type="entry name" value="PHOSPHATIDATE PHOSPHATASE PAH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16876">
        <property type="entry name" value="Lipin_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04571">
        <property type="entry name" value="Lipin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08235">
        <property type="entry name" value="LNS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00775">
        <property type="entry name" value="LNS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LNS2/PITP" evidence="6">
        <location>
            <begin position="558"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="95"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="218"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="304"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="488"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="220"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7733684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="761" mass="85389" checksum="F943C21C2B919EE3" modified="2024-05-29" version="1">MISRVGSYLSQSVYSVATPFHPFGGAVDIIVVQQQDGSFRSTPWYVRFGKFQGVLKGAEKVVKIEVNGVEADFHMYLDNSGEAYFIKEITPEKDNDVKDSNGSLESTKDDFATENNNNVEVQFRDDRAESTESDSEKRFYEFQDEQASSLDGSVDLSDFGSNRVDGQILSAPITSMEENTDNVQLSTPQFHLGPVEAQEFEGGLDLSNESTWAQYLSDLSRPKDKGNETRSDVEKEDETEGVNLEVKSESTAVNSHDTVPKVSDVVINGDQDHESLKLKVENGSIRVSSLDTEPKSSDVEMIKDHDREESEWVENSSNRTEIEDGCTTSVTEEPQSYYDDNENFDRPKQTRIEISLCGNLLRPGMGLSTAAETFNAHRIPEEEFKISAASIIKNDSLIVKIEDKYFTWEKAAHIVLGMAAYGLEMPVEPTDAIPVEWSRPSEDAGLPTTPSGRRWRLWPIPFRRAKTLEHNASNISGEDVFLDSESVFDSPQPVETTPTRSQSPQKQFGRTHVPTTEQISSLNLKDGQNMVSFIFSTRLLGVQKVDAHIYSWKWNARIVISDVDGTITKSDVLGQFMPLVGKDWTQSGVARLFSAIKENGYQLLFLSARAIVQAYLTRSFLINLKQDGKALPNGPVVISPDGLFPSLFREVIRRAPHEFKIACLEDIKALFPSDYNPFYAGFGNRDTDELSYRKIGIPKGKIFIINPKGEVAISHRVDMKGKSYTSLHTLVNDMFPPTSMVEQEDYNSWNYWRVPLPDVEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C4Y6</accession>
    <name>A0AAD5C4Y6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RCC1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_029153</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009551">
        <property type="protein sequence ID" value="KAI7735135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C4Y6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058923">
        <property type="entry name" value="RCC1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051210">
        <property type="entry name" value="Ub_ligase/GEF_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22870:SF155">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE HERC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22870">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00415">
        <property type="entry name" value="RCC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25390">
        <property type="entry name" value="WD40_RLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00633">
        <property type="entry name" value="RCCNDNSATION"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00626">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="RCC1-like" evidence="3">
        <location>
            <begin position="4"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="27"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="126"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="177"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="235"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="288"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7735135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="513" mass="55175" checksum="C0D3DCC9B15DE4A8" modified="2024-05-29" version="1">MDSGNLSRKVTAIATGEAHTLALTGDGKVYSWGKGNFGRLGNASEADQHSPVKIHFNSSDYLKIIGISAGAYHTLALSDGQLGVDGDNSVVPCLVKMPITDENEKPLKISSVKAGGMTSLAIDDLGRLWMWGNLQGISSEMESTPIPTSINFNGHTVIKVSCGGGHIVALVNNGDHLSCYSWGNNDHGQLGLGDTEMRSNPQIIDKFNTLEAYEVACGASHTCVLAYTTKTKTESLCWSFGRGSNGQLGLGTTKDSPSPEMVNGLPGNLLLVSVACGLFHTSVVSSSGNVWSWGMENGLGLCPEATFAEGDGGDALSPRLINGPNFPQPIQVACGAAHTVVVADGGYKLWSWGRGRSGVLGNGQEVDFFSPKAVLWPPPSEEAYPETVGKIMEKHPEELTDMEKKLSAAMEEMNVLRSRLSVMEQYASILHGSIFGRPFDGDRDIPLSLRDSQGAFDITKEWENMLESCDRGKLIRLQMFYRNMLAGVEDKMMKQRIKEMLKEFLGSSAGTSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C6D0</accession>
    <name>A0AAD5C6D0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Diacylglycerol O-acyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_009755</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a long chain fatty alcohol + a fatty acyl-CoA = a long-chain alcohol wax ester + CoA</text>
            <dbReference type="Rhea" id="RHEA:38443"/>
            <dbReference type="ChEBI" id="CHEBI:17135"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:77636"/>
            <dbReference type="ChEBI" id="CHEBI:235323"/>
            <dbReference type="EC" id="2.3.1.75"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>an acyl-CoA + a 1,2-diacyl-sn-glycerol = a triacyl-sn-glycerol + CoA</text>
            <dbReference type="Rhea" id="RHEA:10868"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="ChEBI" id="CHEBI:64615"/>
            <dbReference type="EC" id="2.3.1.20"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Glycerolipid metabolism; triacylglycerol biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Lipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">In the N-terminal section; belongs to the long-chain O-acyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009432">
        <property type="protein sequence ID" value="KAI7735840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C6D0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004144">
        <property type="term" value="F:diacylglycerol O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047196">
        <property type="term" value="F:long-chain-alcohol O-fatty-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019432">
        <property type="term" value="P:triglyceride biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045034">
        <property type="entry name" value="O-acyltransferase_WSD1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009721">
        <property type="entry name" value="O-acyltransferase_WSD1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004255">
        <property type="entry name" value="O-acyltransferase_WSD1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31650">
        <property type="entry name" value="O-ACYLTRANSFERASE (WSD1-LIKE) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31650:SF48">
        <property type="entry name" value="O-ACYLTRANSFERASE, WSD1, O-ACYLTRANSFERASE WSD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06974">
        <property type="entry name" value="WS_DGAT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03007">
        <property type="entry name" value="WS_DGAT_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="O-acyltransferase WSD1-like N-terminal" evidence="11">
        <location>
            <begin position="50"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="O-acyltransferase WSD1 C-terminal" evidence="12">
        <location>
            <begin position="281"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF03007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF06974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7735840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="432" mass="48359" checksum="F81373D64DEA61C2" modified="2024-05-29" version="1">MGIETDEPLSPAGRLFVQPATHQIINSVFGLEHAIGLDALRTVLSDSLLIKHPRFSSLLVTDDHGLEHWRKTELDIDRHIIIRPDPVGEGDNDEEIVNEYIADLTVSCPLNTDKPLWEIHLLSAHKCLVLRVHHALGDGISLASLMLTLCRKLDDPEQTPTIRPLSSSTNKVHKKLGTWDNFMKVLKMVWFSLVYVCEFMMRGAWVKDGKTAVSGGEGVEMWPRKLVTAKFSFEDMKTVKSAVANAEGLQITGVALVNLRPSPGLQDMKELMKKNAGTTGWGNKVGIMLLPVYYHRNRSDPLQYLKRAKMMMDRKKLSMEAVLSHKIGYFVMKYFGAKVASLLEYKVICNTSFTISNVVGPREEITVAGIPITYMRTTSSSLPHAITMHMVSYAGKADMQILVAKDLIPDPEKLAKCFEDALQEMKEQAVQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C7S5</accession>
    <name>A0AAD5C7S5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_020604</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009252">
        <property type="protein sequence ID" value="KAI7736530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C7S5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16461">
        <property type="entry name" value="RING-H2_EL5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000971">
        <property type="entry name" value="Putative RING zinc finger domain superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053070">
        <property type="entry name" value="RING-type_E3_ubiquitin-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47035:SF4">
        <property type="entry name" value="OS02G0676500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47035">
        <property type="entry name" value="OS11G0150450 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="3">
        <location>
            <begin position="71"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="137"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7736530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="156" mass="17918" checksum="A871044DC117D724" modified="2024-05-29" version="1" fragment="single">MSATFIVFVCARLFCGRFRRYESRELFEIDSRIDLERQEHRVVGLEPDIVAAIPTMKFDREAFGSMEDAQCTICLGEYQEKEVLRIMPKCGHSYHLSCIDLWLRKQSTCPVCRLSVIGTSSPEISRDISMERSRQWLLSRPGQGSSQSQREDSAMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C963</accession>
    <name>A0AAD5C963_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Triosephosphate isomerase, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_033821</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="4">Carbohydrate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the triosephosphate isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009290">
        <property type="protein sequence ID" value="KAI7736369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C963"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004807">
        <property type="term" value="F:triose-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046166">
        <property type="term" value="P:glyceraldehyde-3-phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019563">
        <property type="term" value="P:glycerol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00311">
        <property type="entry name" value="TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000025">
        <property type="entry name" value="Triosephosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00147_B">
        <property type="entry name" value="TIM_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035990">
        <property type="entry name" value="TIM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022896">
        <property type="entry name" value="TrioseP_Isoase_bac/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000652">
        <property type="entry name" value="Triosephosphate_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020861">
        <property type="entry name" value="Triosephosphate_isomerase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00419">
        <property type="entry name" value="tim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21139">
        <property type="entry name" value="TRIOSEPHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21139:SF2">
        <property type="entry name" value="TRIOSEPHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00121">
        <property type="entry name" value="TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51351">
        <property type="entry name" value="Triosephosphate isomerase (TIM)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00171">
        <property type="entry name" value="TIM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51440">
        <property type="entry name" value="TIM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7736369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="299" mass="31931" checksum="717ACC65751E9C1D" modified="2024-05-29" version="1" fragment="single">MATMLSTCPQLWGPNSAPLFSGLRRSSTSTQSFFQPSSSSSSRPSRTIIAMAGTGKFFVGGNWKCNGTKDSIKQLVSDLNSATLEPDVDVVVGPPFVYIDQVKSSLTDRIEIAAQNSWIGKGGVFTGEISAEQLKDIGCKWVILGHSERRHVIGEDDQFIGKKAAYALGQNLGVIACIGELLEEREAGKTFDVCFKQLKAYAGRSSSSILLVVNAVPSWDNIVIAYEPVWAIGTGKVASPEQAQEVHVAVRDWLAKNVSAEVASKTRIIYGGSVNAGNSAELAKKEDIDGFLVGGASLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-06-18" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C9G5</accession>
    <name>A0AAD5C9G5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">HSF-type DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_011419</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Homotrimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the HSF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009094">
        <property type="protein sequence ID" value="KAI7737195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000037">
        <property type="entry name" value="Heat stress transcription factor B-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000232">
        <property type="entry name" value="HSF_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015">
        <property type="entry name" value="HEAT SHOCK TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015:SF329">
        <property type="entry name" value="HEAT STRESS TRANSCRIPTION FACTOR B-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00447">
        <property type="entry name" value="HSF_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00056">
        <property type="entry name" value="HSFDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00415">
        <property type="entry name" value="HSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0346">Stress response</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HSF-type DNA-binding" evidence="11">
        <location>
            <begin position="6"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="103"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="119"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7737195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="279" mass="31341" checksum="3C357D09121E77E4" modified="2024-05-29" version="1">MTHRSAPAPFLSKTYSLVDDPTTDDVISWNESGTTFVVWKTADFAKDLLPNSFKHNNFSSFVRQLNTYGFRKTVPDKWEFANENFKRGQRELLLKICRRKTVTTSQSPTNGKPADNDRPTSSPTSSGEELGSSSTSSPDSKXXXXXXXXXXXXXXKLKKEKLILTSELAQMKKQCDDLVAFLTQNVKVAPDQINRIMNGDIVVDDTKASGDDESDDDEKRNSFKLFGVLVKEEKKKRGRNEINDSLGAKRKEMKMNKFNKSASEMNVYAITGQSTKVCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C9J8</accession>
    <name>A0AAD5C9J8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Response regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_017051</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008982">
        <property type="protein sequence ID" value="KAI7737617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C9J8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009736">
        <property type="term" value="P:cytokinin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045279">
        <property type="entry name" value="ARR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43874">
        <property type="entry name" value="TWO-COMPONENT RESPONSE REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43874:SF205">
        <property type="entry name" value="TWO-COMPONENT RESPONSE REGULATOR ORR23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="1" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="Response regulatory" evidence="5">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="4">
        <location>
            <position position="26"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7737617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="50" mass="5832" checksum="D9F95C5D673E2273" modified="2024-05-29" version="1" fragment="single">TTTNQARTALKMLRENRKRFDLVISDVHMPDMDAVLQSQQLLHARKQVLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C9X0</accession>
    <name>A0AAD5C9X0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_011925</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DRM1/ARP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008893">
        <property type="protein sequence ID" value="KAI7738016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C9X0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008406">
        <property type="entry name" value="DRM/ARP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33565">
        <property type="entry name" value="DORMANCY-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33565:SF23">
        <property type="entry name" value="DORMANCY_AUXIN ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05564">
        <property type="entry name" value="Auxin_repressed"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="32"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="68"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="78"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7738016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="121" mass="13026" checksum="D51B0087C08CABE8" modified="2024-05-29" version="1">MSLLNKLWDDTIAGPPPDKGLGKLRKQNLSFRSLNSEKESETGGNTSAAVEYPAAKVTRSIMIVKPERTLSETPPASPAGSTTPVSPFSGESFRFRRKSASDAFEKASGIGSRSPRPPYEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CB86</accession>
    <name>A0AAD5CB86_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_022836</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008916">
        <property type="protein sequence ID" value="KAI7737990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CB86"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051504">
        <property type="entry name" value="Plant_metabolite_acyltrans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31625">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02458">
        <property type="entry name" value="Transferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="803"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7737990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="803" mass="89798" checksum="DFD01ED6C43BBF80" modified="2024-05-29" version="1" fragment="single">KEMDSLPNLTVLEQSEVSPPPAAIGRRSLLLTFLDIVWLAHSEPAHSLFFYELQLITRTQFTQTIIPNLKQSLSLTVTFAECNNLDYPKFIMLKCNNLDFNDLIGNHPRECDKFYPLIPLLGRTTKVSDYITIPVFAVQVTLFPNHGISIGMTNQHSLGDANAMFCFLKAWTSIAQSGGVDELFLTNGTLPIFDRLVETYNEEYKLPRLSGPTDKVRATFILSRITISGLKKLVSTQLPTLAYISSFTVACAYIWNCLAIFVFAVDCRSRTDPPIPEAYFGNCLMPCLNVARAFVLTGKEGFLAAAELIGENLNKMLTDKEGVLKEKEPMENLFVDGPPTRITGDTCLSLISTNTIHALHYTNTKPTYYTFTTCPLIFPFVMMDGPTTFANQCSKLNNKNMHSMGRREWRPPWRAVETAPNAVGKVEWRPPWQPTSRFDEATPNAFVRLEWRPEASEDLEIGVCLSATEMDVFVGSFKSGLELALTLFEGGCMITIEKRAHWEIGRVLVRLDLIEGYGAPGNPNGVGILIFDGGVLLCVATQKEMDPLSNLTVLEQSEVLPPPAAIGCRSLLLTFLDIVWLAHPEPTHTLFFYELPMITRTHFTQTIVPSLKQTLSLTLQHFFPFAVFAVQVTLFPNCGISIGITNHHSLCDTSTMFCFLKAWTSIAQSSGSNESFLANGTLPIFDRLLKYPKRDESCLKNVKVEETYNEEYKLPRLSGPTDKVRATFTLTRTTINRLKKLVSTQEKKAAAELIGKNLNKMLTDKEGVLKENGPMENLFVDGPPTRITGVSGTPKFKFYDLDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CBC0</accession>
    <name>A0AAD5CBC0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_021093</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008720">
        <property type="protein sequence ID" value="KAI7738796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CBC0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7738796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="34" mass="3914" checksum="CEC981BBFBCE7810" modified="2024-05-29" version="1">MVNTTRIILQKQMLHFCVRGARIQVDALAKLTMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CBI7</accession>
    <name>A0AAD5CBI7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">M8C21_008408</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">AA19_3_7</strain>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the monovalent cation:proton antiporter 2 (CPA2) transporter (TC 2.A.37) family. CHX (TC 2.A.37.4) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008822">
        <property type="protein sequence ID" value="KAI7738345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CBI7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006885">
        <property type="term" value="P:regulation of pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057291">
        <property type="entry name" value="CHX17_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057290">
        <property type="entry name" value="CHX17_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050794">
        <property type="entry name" value="CPA2_transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32468">
        <property type="entry name" value="CATION/H + ANTIPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32468:SF18">
        <property type="entry name" value="CATION_H(+) ANTIPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23256">
        <property type="entry name" value="CHX17_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23259">
        <property type="entry name" value="CHX17_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0630">Potassium</keyword>
    <keyword evidence="3" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="51"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="84"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="112"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="147"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="180"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="211"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="251"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="292"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="330"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="391"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="12">
        <location>
            <begin position="31"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H(+) antiporter central" evidence="13">
        <location>
            <begin position="471"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H(+) antiporter C-terminal" evidence="14">
        <location>
            <begin position="613"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="484"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="490"/>
            <end position="506"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF23256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF23259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAI7738345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="776" mass="86063" checksum="BD51A05234788B29" modified="2024-05-29" version="1">MDETSELSCPENNMMNPALTMTTQVSAILVLSHVFQIFLKPLGQPAPLAQILAGFVLGPSGLSRAPSIHVFFFQNFAVDFYESMALYCRISIMFLLGLEMDISYLMRNLRPVIIIACGGCLMCSIFASAISLFVYEETGSHGSRFTLALVIIVVLANTASPIVFRLAADLKFATSDIGRLAISTSLVGDMYAVFILVMISGEKHKQTVNGWALMAFCSLLLLVGIIIFTSYLVNWLNKRNRDEKNLQAVEVMLLCLVILVAAMTLETMGFSSIIACFLMGSTFPRGGKSARTLLAFLTYMVRNFVFPIYFGYTGFKADTSYLNEIKRVAIVLMVIVLSIGGKILGTLAACWYLKTPLNEGVLLAFLMNMKGHVDILTLTTAMQNKVLNSPLFYNLMVSAIVISSLISASLISFLVRRESDTIGAKHIPMEYHTPDKELRLLTCVHSRHPVDCMVKLIVSLRGSDNVPISPYLMHLVELPEKTKKSSSFYDQEDDEFSDDEDDDSNDDVEINASVDMYIEETKIMIHQAKTVAPFLTMYQDVCDFSDDIRASFIVLPFHKHQRIDGKFENDKDGIRTTNQKVLRNAQCSVAILVDRAHTTTGTQAAGSESLQQIAILFFGGPDDREALGFSKRLSTHHHSSVTVFRFLPTSAKDPNEGINVSQKEEDVVVTIQDQQIEVDEADSSALANFYHRYVTSGKVRFVEKYVRNGAETGMVLREMAETYSMFIVGKGGRGDCLLTTGISDWEECPELGVVGDFLASSEFDISGSILVVQQQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CCL3</accession>
    <name>A0AAD5CCL3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="2">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_005001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010008626">
        <property type="protein sequence ID" value="KAI7739456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CCL3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46463:SF27">
        <property type="entry name" value="OS03G0788800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46463">
        <property type="entry name" value="ZINC FINGER, RING/FYVE/PHD-TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17123">
        <property type="entry name" value="zf-RING_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="9">
        <location>
            <begin position="30"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7739456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="60" mass="6503" checksum="6705B36D19C8E0EF" modified="2024-05-29" version="1">MEESTKSKDHLISAAAFVEAGAQDACDDACSICLEAFSDSDPPTVTGCKHEFHLQCILEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CCY3</accession>
    <name>A0AAD5CCY3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">GTD-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_030427</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008631">
        <property type="protein sequence ID" value="KAI7739342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CCY3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080115">
        <property type="term" value="F:myosin XI tail binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007656">
        <property type="entry name" value="GTD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039306">
        <property type="entry name" value="MYOB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31448:SF52">
        <property type="entry name" value="MYOSIN-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31448">
        <property type="entry name" value="MYOSIN-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04576">
        <property type="entry name" value="Zein-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51775">
        <property type="entry name" value="GTD_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="GTD-binding" evidence="7">
        <location>
            <begin position="368"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="251"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="367"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="254"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7739342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="720" mass="82684" checksum="CBE1302C5620853B" modified="2024-05-29" version="1">MSGVHDMCYDCSSGVGERSNFYVFLGEKIEKKIEFVEKDDVCEVGLKCSCCGVEFIRKGVEFDISNNNDNNNDNDNNNNSSCFVINSSWDLLKFEKDLNSVVDVIGSGEKYDLEDVNEVKFVVKETEETEETEDLIKFHEKEGQDLEFLLDYSGNQLVSIESIDEDHEFGDFQKAQDVLESKTEIVTEDLLQEKEERYSVLEDADEKRDELSKFVELDSMEFEETENSLVFHVNSCDFTVEKPAISETIQASSDSKDVQHIEGSDSDHEEAEVSIGTEIPVLDSCDEIKAQDNFNLYTLSHEEEPSSSSHDLDFNLESGFQEARDDEKLEELKNLNQEVDTLLVIERKDSMVDESFDGSEMDGVDPINTTEKLKSALRAERKALQELYTELEEERSASAVAASETMAMINRLQEEKAAMQMEALHYQRMMEEQSEYDQEALQLLNELMVKKEKELELYRKKVLDYETKERMRFLSTSTKSGTCSASCSHSEDGDGMWVDLSHEPKEESRNHNTPVDTIMNLDSSFMDFEDERLSILEQLKVLEEKLFALSDEEDRHFSNFRQIEDYFEENGKHLNNGQQVNGIANGFPKEPLETRYQDRRNIGSTGKRLLPLFDALENESDEGVITSNGHENGFHSDKVEDVAITRFELQKKRIDIEEEVDQLYVRLQALEADREFLKHCIGSLKKGDKGMELLQEILQHLRDLRNVDLREKSFTDGTLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CEB6</accession>
    <name>A0AAD5CEB6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_025780</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008531">
        <property type="protein sequence ID" value="KAI7740032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CEB6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00018">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.730.10:FF:000002">
        <property type="entry name" value="AP2-like ethylene-responsive transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.730.10:FF:000003">
        <property type="entry name" value="AP2-like ethylene-responsive transcription factor ANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.730.10">
        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001471">
        <property type="entry name" value="AP2/ERF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32467">
        <property type="entry name" value="AP2-LIKE ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32467:SF248">
        <property type="entry name" value="AP2_ERF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00847">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00367">
        <property type="entry name" value="ETHRSPELEMNT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00380">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51032">
        <property type="entry name" value="AP2_ERF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="7">
        <location>
            <begin position="235"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="AP2/ERF" evidence="7">
        <location>
            <begin position="337"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7740032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="475" mass="52932" checksum="7BFCD742D43A1BB5" modified="2024-05-29" version="1">MTTNNINDNNKNNDNWLGVSLSPSNPTSFFTLPSNFNYQNIYYGGVECVNGGGIYSHNFPTMPLKSDGSLGMVATSTPKLENFFGGVTMGTPGNLDNNASTMYYHQNNYHIQPQHYQDYSGLYPTLQQHEAKIVANTTCGLYLPSIGENWIANSYHSGPSGDGLQSLRLSMSPGCSQSSSCVTVSQQQMKPSAAANNITETVVMDTKKRGSGKVNQQKQIVHRKSLDTFGQRTSQYRGVTRHRWTGRYEAHLWDNSCKKEGQTRKGRQVYLGGYDMEEKAARAYDLAALKYWGPSTHINFPLENYEQELEEMKNMSRQEYVAHLRRRSSGFSRGASIYRGVTRHHQHGRWQARIGRVAGNKDLYLGTFSTQEDAAEAYDVAAIKFRGTNAVTNFDMTRYNVEKIIQSNTLLTGELARRTKDPGTTNQVIPNQEPTNESISIVGSKAMNEWIPTAAAATTGEVRSQVPIFGAWTDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CF58</accession>
    <name>A0AAD5CF58_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Alpha-1,3-glucosyltransferase</fullName>
            <ecNumber evidence="10">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_010729</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2 10">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Endoplasmic reticulum membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the ALG6/ALG8 glucosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="10"/>
    <dbReference type="EMBL" id="JAMZMK010008628">
        <property type="protein sequence ID" value="KAI7739386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CF58"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042281">
        <property type="term" value="F:dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004856">
        <property type="entry name" value="Glyco_trans_ALG6/ALG8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12413">
        <property type="entry name" value="DOLICHYL GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12413:SF1">
        <property type="entry name" value="DOLICHYL PYROPHOSPHATE MAN9GLCNAC2 ALPHA-1,3-GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03155">
        <property type="entry name" value="Alg6_Alg8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 10" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="162"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="254"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="320"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="344"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="406"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="435"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="468"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7739386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="509" mass="58834" checksum="06C9690529FAC1C8" modified="2024-05-29" version="1">MLVILAFCAYKASLSKKIRYVSDSKEMKKSRVTKAVKVEINNNNAWWWLTNTRISAPFICVSLFAILVRIAVSLHPYSGAGDPPKYGDFEAQRHWMEITTNLPVNEWYQNTTRNDLGYWGLDYPPLTAYQSYVHGLVLRFFHPESVSLYNSRGHESYFGKLLMRWTVLSSDVLIFFPAVLWLIIAFADQSKGHKRLTVGAIAAVLCDKDLVASFLFSLALNHKQMSAYYAPAFFSYLLGKCLKRQNPLLEVSKLGMIVIGTFALVWWPYLQSRDAVLEVLSRLAPFERGLYEDYVANFWCTTSVLIKWKRLFTTQSLKLLCLFMTVCSCLPSMFLQIRAPSPRAFLYGLLNSAFSFYLFSFQVHEKSILLPLLPASILAIEEPFVYQWFTQFALLSMFPLLCRDKLLLPYIALYGIFIFIYYAPNGKPDTPKPRFWSGVTFFVVFLLFLSFVLHIIYLTVDPPKKYPFLFEAIIMLFCFSQFILVVVYTNWKQWILSRKSPVGSEKKRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CGX9</accession>
    <name>A0AAD5CGX9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cytochrome P450 78A5-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_026506</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008144">
        <property type="protein sequence ID" value="KAI7741706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CGX9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000016">
        <property type="entry name" value="Cytochrome P450 78A5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051996">
        <property type="entry name" value="Cytochrome_P450_78A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47946">
        <property type="entry name" value="CYTOCHROME P450 78A7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47946:SF13">
        <property type="entry name" value="CYTOCHROME P450 FAMILY PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8 9" id="KW-0349">Heme</keyword>
    <keyword evidence="6 8 9" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="7"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="8">
        <location>
            <position position="464"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7741706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="524" mass="59176" checksum="5B6E680E82B9B3E7" modified="2024-05-29" version="1">MKIFVKGGFLPLVICFVIGFYNLSLPHLFLISFVSLFPFFVNIWLVPGGFAWRNPNLNPTKIPGPVGWPFLGILPKMGPHAHSKLAFIASSMGATRLMAFSLGSTRVVITSHPETAKEILCGSAFSDRPIKESASLLMFERAIGFAPSGQYWRHLRRIAANHMFSPKRVSSLECLRQRVCDQMMEKVSKEMSVRGVVELRGILQKGSLRNVIESVFGSGLGLEIEEELGFMVKEGYELISEFNWGDYFPIRVLDFTGVKRRCHKLSLKVQRLVGQIVEETRRDINTQGTSNDRNDFLSVLLSLPGEDQLNHPDMVAVLWEMIFRGTDTVAILLEWIMARMVLHQDIQAKAQDEIKELVGEHRLVQDSDIPNLGYVQAIVKEVLRLHPPGPLLSWARLSTRDVQLGKFFVPAGTTAMVNMWAITHDPSIWKNPWDFKPERFMEEDFPIMGSDLRLAPFGSGRRVCPGKSLGLATVNLWLARLLQQYKWLPSTKVDLSECLKLSLELKTPLKCLAVMRCEHTSSTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CHT0</accession>
    <name>A0AAD5CHT0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_001616</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008065">
        <property type="protein sequence ID" value="KAI7742157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CHT0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000394">
        <property type="entry name" value="MYB transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015495">
        <property type="entry name" value="Myb_TF_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47994">
        <property type="entry name" value="F14D16.11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47994:SF5">
        <property type="entry name" value="F14D16.11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="20"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="20"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="82"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="95"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7742157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="229" mass="25994" checksum="CC005CCEF57340D3" modified="2024-05-29" version="1" fragment="single">LAGLRRCGKSCRLRWTNYLRPDLKRGLLNEYEEQLVIDLHARLGNRWSKIAANMPGRTDNEIKNHWNTHIKKKLLKMGIDPVTHKRLQKEPEADMTSSSSAKQLMQHEYSSPSASATTLQSYEQNSSCTMSENSIAIQDQGTQANLLENLSEDEKLLSYLLGEDEPPLVDTSTWKLPNTGPSFNDRSDGFASWDDCVTWLLDCQDFGVHDFGMDTFNDVEISIIDIVNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CIK7</accession>
    <name>A0AAD5CIK7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Serine hydroxymethyltransferase</fullName>
            <ecNumber evidence="12">2.1.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_021154</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the interconversion of serine and glycine.</text>
    </comment>
    <comment type="function">
        <text evidence="12">Interconversion of serine and glycine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + glycine + H2O = (6S)-5,6,7,8-tetrahydrofolate + L-serine</text>
            <dbReference type="Rhea" id="RHEA:15481"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="EC" id="2.1.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 11 12">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 12">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 12">Belongs to the SHMT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.1" evidence="12"/>
    <dbReference type="EMBL" id="JAMZMK010008265">
        <property type="protein sequence ID" value="KAI7741139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CIK7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004372">
        <property type="term" value="F:glycine hydroxymethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019264">
        <property type="term" value="P:glycine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00378">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000050">
        <property type="entry name" value="Serine hydroxymethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00051">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001085">
        <property type="entry name" value="Ser_HO-MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049943">
        <property type="entry name" value="Ser_HO-MeTrfase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019798">
        <property type="entry name" value="Ser_HO-MeTrfase_PLP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039429">
        <property type="entry name" value="SHMT-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000586">
        <property type="entry name" value="PRK00011.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680:SF63">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE 3, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00464">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000412">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00096">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 12" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="8 11 12" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Serine hydroxymethyltransferase-like" evidence="13">
        <location>
            <begin position="82"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="11">
        <location>
            <position position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00059150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000412-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7741139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="529" mass="57901" checksum="23DB0217AD3CEF7D" modified="2024-05-29" version="1">MHTCTGVIMGSLQQPVSIRESNFLPKGPCVSRLLRQVNLGSFKLCRSLKIEGSLATGKSSTPSVSVTIPEGGNGSNFVDHELSNVDPEVSEIIYNEKQRQFRSLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYGGNEHIDELEILCQKRALAAFHLDENKWGVNVQPLSGSPANFEVYTAILKPHDRIMGLDLPHGGHLSHGFMTPKRRVSGTSIYFESMPYRLDEATGLVDYDMLEKTATLFRPKLIIAGASAYPRDFDYPRMRKIADSVGAFLMMDMAHISGLVAASVVGNPFEYCDIVTTTTHKSLRGPRGGMIFFKKDPVLGIELESAINNAVFPGLQGGPHNHTIGGLAVCLKHAQSPEFKAYQNQVVSNCRALAKRLMELGYTLVSGGSDNHLVLVDLRPLGLDGARVEKILDLVSITLNKNSVPGDKSALVPGGIRIGSPAMTTRGFTEQEFIYIAEFIHEGVQLTREVKQAVSGSKLQDFMKFVASLDFNSFTDKVSDLRTRVEALTTQFPIPGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CJB6</accession>
    <name>A0AAD5CJB6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF4378 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_006327</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008171">
        <property type="protein sequence ID" value="KAI7741559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CJB6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025486">
        <property type="entry name" value="DUF4378"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47212">
        <property type="entry name" value="ADHESIN-LIKE PROTEIN, PUTATIVE (DUF3741)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47212:SF10">
        <property type="entry name" value="DUF4378 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14309">
        <property type="entry name" value="DUF4378"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4378" evidence="2">
        <location>
            <begin position="413"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="256"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="261"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7741559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="527" mass="60159" checksum="E485EC477F31491E" modified="2024-05-29" version="1">MLTFDEQIRSIYINSKKKAASVNQSLKKEEAWKKTVEKCFWRKMKTRYENMSKKKENTNVIDTVVVLKPSRRVMDCPVDVGCRCLHSHPHHNSASKKHMVKHSHVLFNDVRKKLKISKLKSLRKMKSCGESDETGFKAGNLSLVPYTKHQEPEVFMEARRHLAERLRLVALGTGVDEPPGSSCKQVARTLERILRTSPIHESMAVLEREMERTYGEKSLHTTWSALSPLKQNEYSSSSGFLEITNTNDFLSRGSKFRDSDPNNIGPSPQGTNGCIEGPQQDLLLDVISPKSGCKPENMDNDQFRENPSPVSVLDPFFSDNFDSPTSTIESGTYIYNNHKFLSTVITNCLMYIIDLVPVEHHIQPRRLDFEEHSSQTSSPPQKSGASFFPEDRVFISSYVKELYQASESNWEDFLSTDYAFESPSDVKVLHDCVREVLVSLHSRMMLFSTRVQPFSLEKDVINKVIDQVDWHNGQPVGPWTLDQLIRMDMAKFGSWLDTLSERNDIMVEAADDILHDLVIEASIDICF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CKN6</accession>
    <name>A0AAD5CKN6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_004119</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007700">
        <property type="protein sequence ID" value="KAI7743671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CKN6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00265">
        <property type="entry name" value="MADS_MEF2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1810.10">
        <property type="entry name" value="Transcription factor, MADS-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050142">
        <property type="entry name" value="MADS-box/MEF2_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033896">
        <property type="entry name" value="MEF2-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002487">
        <property type="entry name" value="TF_Kbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002100">
        <property type="entry name" value="TF_MADSbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036879">
        <property type="entry name" value="TF_MADSbox_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48019">
        <property type="entry name" value="SERUM RESPONSE FACTOR HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01486">
        <property type="entry name" value="K-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00319">
        <property type="entry name" value="SRF-TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00404">
        <property type="entry name" value="MADSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00432">
        <property type="entry name" value="MADS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55455">
        <property type="entry name" value="SRF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51297">
        <property type="entry name" value="K_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00350">
        <property type="entry name" value="MADS_BOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50066">
        <property type="entry name" value="MADS_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041928402" evidence="7">
        <location>
            <begin position="26"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="MADS-box" evidence="8">
        <location>
            <begin position="72"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="K-box" evidence="9">
        <location>
            <begin position="155"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="148"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7743671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="300" mass="34941" checksum="48934498FC3366DE" modified="2024-05-29" version="1" fragment="single">HICSNTSLFWKLFLFTFFLFPFTQTQNTHTNTHFIFYIFIPYPPHILPSISNLLHLLLPQIQTTNNISIINMARGKIQIKKIENSTNRQVTYSKRRNGLFKKANELTVLCDAKVSIIMVSCTEKLHEYISPSITTKQFFDQYQKVSGIDLWNSHYEKMQEELRQLKDVNTNLRRQIRQRLGDCLENAGFEELLDLEKESQEAVYIIRERKIKVIGNKLETCKKKVRSAQDVYKKLMHEFDIRGEDPQYGMIEDAGEYEALYGYPPHIAAAQRILTLRLQPNHPNDIHAAASDLTTYALLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CNH3</accession>
    <name>A0AAD5CNH3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tropinone reductase-like 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_009276</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007420">
        <property type="protein sequence ID" value="KAI7744899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CNH3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005559">
        <property type="entry name" value="PRK07231.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43943">
        <property type="entry name" value="DEHYDROGENASE/REDUCTASE (SDR FAMILY) MEMBER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43943:SF2">
        <property type="entry name" value="DEHYDROGENASE_REDUCTASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7744899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="255" mass="27114" checksum="47ECEAF9951C6B3F" modified="2024-05-29" version="1">MEKSKLGRRFEGKIAIVTASTQGIGFSIAQRLGIEGASVVISSRKQRNVDEAVGKLKAQGIDVLGLVCHVSNAQQRKNLIDQTVQKYGKIDVVVLNAAVNPSVNPILQTKESTLDKLWEVNVKTSILLLQDASPHLSNGSSIVFTSSISAFQPPPGMAMYGVTKTTLLGLTKALATELAPNIRVNCVAPGFVPTHFASYITSNETKRKALEEMTSLKRLGTTEDVAAATAFLASDDASYITGETIVVSGGTPSRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CNM3</accession>
    <name>A0AAD5CNM3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_017315</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007538">
        <property type="protein sequence ID" value="KAI7744435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CNM3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.80">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003441">
        <property type="entry name" value="NAC-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036093">
        <property type="entry name" value="NAC_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31744:SF233">
        <property type="entry name" value="NAC DOMAIN-CONTAINING PROTEIN 72-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31744">
        <property type="entry name" value="PROTEIN CUP-SHAPED COTYLEDON 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02365">
        <property type="entry name" value="NAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101941">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51005">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NAC" evidence="6">
        <location>
            <begin position="15"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7744435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="222" mass="25317" checksum="D43418071900DB04" modified="2024-05-29" version="1">MEPINPNNNPTLSPLPPGCQFFPSEQQLITHYLTLKNTGDHLDPNSPNLITEIDLYRFDPFNLPDTARFLFGREGKRKHWYCFVPMPRVKRGRGEKRPAGCGFWRRSGSVKDIVGSGIQGVVVGTRKTFVFYFGETCKTAVRTKWVLYEYSLVDQKMAEASYVLCRVFAKSHHRCNTSNDKRKPLNETVKTVSHIGIQCDEKVNSLAFEGDFLELNDLLSPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CQ18</accession>
    <name>A0AAD5CQ18_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glycosyltransferase</fullName>
            <ecNumber evidence="6">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_007298</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">May glycosylate diterpenes or flavonols in leaves.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="6"/>
    <dbReference type="EMBL" id="JAMZMK010007093">
        <property type="protein sequence ID" value="KAI7745877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CQ18"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080043">
        <property type="term" value="F:quercetin 3-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080044">
        <property type="term" value="F:quercetin 7-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000056">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000065">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926">
        <property type="entry name" value="GLUCOSYL/GLUCURONOSYL TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926:SF1417">
        <property type="entry name" value="UDP-GLUCURONOSYL_UDP-GLUCOSYLTRANSFERASE, UDP-GLYCOSYLTRANSFERASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00053747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7745877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="480" mass="54169" checksum="E135A04E30A45764" modified="2024-05-29" version="1">MDPMLTTEKKPHVVCIPLPSQSHVKAMLKLAKLLHHKGLQITFVNTEFVHQLLLESGGPHSLDGSPGFHFETIPDGVPRSSKDSITTTRLLLLQSVETNLLAPFIDLVTKLPNPPTYIISDGFMSAFTIDAAQNLGIPVMMYWTFAACAIMGFYQINSLIEKGLAPLKDESYLTNGYLDTVIEWVPGMEDIRLKDFPMTWTTDANDKQLVFSKEAPQRSVKVPHHIFHTFDELETSTIKALSSIYSRVYTIGPLQLLLDQIPREKMQSESSSTHGYSLWKEEPECFQWLQSKEQNSVIYVNFGTTTEMSLNDVRELGWGLANSNHYFLWIIRSNLVEGESAVLPPEFEEHINKRGFIASWCSQEKVLNHPSVGGFLTHCGWGSTIESLSAGVPMICWPFAWDQKTNRRSICKDWGVGVEMGNEVKRDEVSRLVQELMGERGHIMRNKAKEWKEKARVATSPNGSSSLNVDNIVKEIMIHC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CQ19</accession>
    <name>A0AAD5CQ19_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">LOB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_022186</name>
        <name evidence="5" type="ORF">M8C21_030698</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LOB domain-containing protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007154">
        <property type="protein sequence ID" value="KAI7745709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAMZMK010002326">
        <property type="protein sequence ID" value="KAI7754878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CQ19"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004883">
        <property type="entry name" value="LOB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31301:SF58">
        <property type="entry name" value="LOB DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31301">
        <property type="entry name" value="LOB DOMAIN-CONTAINING PROTEIN 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03195">
        <property type="entry name" value="LOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50891">
        <property type="entry name" value="LOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LOB" evidence="3">
        <location>
            <begin position="20"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="128"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7745709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7754878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="176" mass="19285" checksum="1B7991170118F2FA" modified="2024-05-29" version="1">MKDVGSSGHGGSGRKQGAAAPCAACKLLRRRCSQDCVFAPYFPADEPHKFASVHKVFGASNVNKMLQELPEHHRGDAVSSMVYEANARIRDPVYGCVGAISSLQQQIDVLQTQLAVSQAEVVHMRMRQYSSNQSPENISPSSRHHQSQTPTRSLFSMDMVDQTNMGMGESLWSSCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CQ57</accession>
    <name>A0AAD5CQ57_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">K-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_010390</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007525">
        <property type="protein sequence ID" value="KAI7744486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CQ57"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002487">
        <property type="entry name" value="TF_Kbox"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01486">
        <property type="entry name" value="K-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51297">
        <property type="entry name" value="K_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="K-box" evidence="3">
        <location>
            <begin position="26"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="122"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="26"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="134"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7744486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="179" mass="20119" checksum="C3BC5EEAED8CF5C3" modified="2024-05-29" version="1">MPEMLGRYKLHSDKNIDKADEPSLNLQLVENDEVRLNKEINDKNQELRQLSGQDLQELTIDELEHLENLLQGGLNRVIQTKDEKIGNEISNLQMKVGAKLMEENKLLKEQARVIKFMTMLSNGKKPRSMRTDARNLTSNPEDQGQSSESVTTNVCSCNSGPPPDDDGSITSLKLGLPFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CQX5</accession>
    <name>A0AAD5CQX5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">CAAX prenyl protease</fullName>
            <ecNumber evidence="14">3.4.24.84</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">M8C21_020492</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">AA19_3_7</strain>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Proteolytically removes the C-terminal three residues of farnesylated proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 14">
            <text>Hydrolyzes the peptide bond -P2-(S-farnesyl or geranylgeranyl)C-P1'-P2'-P3'-COOH where P1' and P2' are amino acids with aliphatic side chains and P3' is any C-terminal residue.</text>
            <dbReference type="EC" id="3.4.24.84"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13 14">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="13 14">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Endoplasmic reticulum membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the peptidase M48A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.84" evidence="14"/>
    <dbReference type="EMBL" id="JAMZMK010007345">
        <property type="protein sequence ID" value="KAI7745159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CQX5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071586">
        <property type="term" value="P:CAAX-box protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07343">
        <property type="entry name" value="M48A_Zmpste24p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2010.10:FF:000002">
        <property type="entry name" value="CAAX prenyl protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2010.10">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027057">
        <property type="entry name" value="CAXX_Prtase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001915">
        <property type="entry name" value="Peptidase_M48"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032456">
        <property type="entry name" value="Peptidase_M48_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10120">
        <property type="entry name" value="CAAX PRENYL PROTEASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01435">
        <property type="entry name" value="Peptidase_M48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16491">
        <property type="entry name" value="Peptidase_M48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 13 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 14" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2 14" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7 13 14" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="70"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="109"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="CAAX prenyl protease 1 N-terminal" evidence="16">
        <location>
            <begin position="2"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M48" evidence="15">
        <location>
            <begin position="128"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="199"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="12">
        <location>
            <position position="242"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR627057-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR627057-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU366005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF01435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF16491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAI7745159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="300" mass="34728" checksum="3E694085EDB0B4D3" modified="2024-05-29" version="1">MFEKYRDYNLDKSTFHLVKEFVTIVTESVILFFGFTPWLWKKSGDVLEIAGLDAENEIYQTLAFLAGAKIWYQITDLPFSLFLAFVIEEDHGFNKQTIWMFIRDMFKDMIVAIILGPPYVAAIILILPLPQGELRTKIETLASSLNFPLKNLYVDRSTRSSDRNVYMYGLFKNQRIVLFNTLIQQCKSEEEVVAVIAHELGHWKMNHTTYSFIAFQLTTMPIQHLVSFGRNLLTRAFDFQADAFAMKLGYAKPLRDRLVQLQEGALSEMNTDPWYSAYHYSSPLLVERVAALDEADKKED</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CR14</accession>
    <name>A0AAD5CR14_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_018869</name>
        <name evidence="9" type="ORF">M8C21_025228</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011050">
        <property type="protein sequence ID" value="KAI7729178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAMZMK010007177">
        <property type="protein sequence ID" value="KAI7745665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CR14"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000001">
        <property type="entry name" value="Phospholipid-transporting ATPase IC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42861">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-1278">Translocase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7729178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7745665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="133" mass="14319" checksum="E954B3DF033B01F3" modified="2024-05-29" version="1" fragment="single">MARLNAIVRSLPSVETLGCTTVICSDKTGTLTTNMMSVSKICVLQSASHGVVAAEYSVSGTTYAPEGSIFDGMGLQLDFPAQFPCLLHIAMCSALCNESVIQYNPDKKKYEKIGESTEVALRILAEKVDSHIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CR41</accession>
    <name>A0AAD5CR41_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_009264</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the polyadenylate-binding protein type-1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006916">
        <property type="protein sequence ID" value="KAI7746701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CR41"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1900.10:FF:000003">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1900.10">
        <property type="entry name" value="c-terminal domain of poly(a) binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036053">
        <property type="entry name" value="PABP-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002004">
        <property type="entry name" value="PABP_HYD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052462">
        <property type="entry name" value="SLIRP/GR-RBP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48027">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN 87F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00658">
        <property type="entry name" value="MLLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00517">
        <property type="entry name" value="PolyA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63570">
        <property type="entry name" value="PABC (PABP) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51309">
        <property type="entry name" value="PABC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="RRM" evidence="11">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="domain" description="PABC" evidence="12">
        <location>
            <begin position="203"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="129"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="137"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="147"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7746701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="297" mass="32054" checksum="9CEE836300D0AD7C" modified="2024-05-29" version="1">MRDPNGTSKGSGFVAFSTSEEASRALMEMNGKMLAGKPLYVALAQRKEDRRARLQAQFSQMRPIAMAPAGAPRMPMFPPGGPGMGQQMFYGQAQPTFIPPQPGFGYQQQLVPGMRPGGGPLPNFFMPMVPPGQQGQRPGGRRGGVPGQQNQQQPVPIMQQQMMPRGRVYRYPPGRDVSMGSVPYDMGNAMPLRDTAGISQPIPIGALASALANASPTEQRTMLGENLYPLVEQLEAESAAKVTGMLLEMDQTEVLHLLESPEALKAKVAEAMEVLRNVAQQQQPADQLAALSLNDLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CRB9</accession>
    <name>A0AAD5CRB9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_011910</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006989">
        <property type="protein sequence ID" value="KAI7746384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CRB9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016125">
        <property type="term" value="P:sterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11043">
        <property type="entry name" value="CYP90-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000022">
        <property type="entry name" value="Taxadiene 5-alpha hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24286:SF209">
        <property type="entry name" value="BETA-AMYRIN 28-OXIDASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24286">
        <property type="entry name" value="CYTOCHROME P450 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0349">Heme</keyword>
    <keyword evidence="8 10 11" id="KW-0408">Iron</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="7 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="10">
        <location>
            <position position="431"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7746384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="484" mass="54987" checksum="3F12A3A7B93BCAC8" modified="2024-05-29" version="1">MIMIMIRLLPIPIFLIIISYVLWSLYSILKSKTNKVNLPPGTFGWPILGESLSLLRAGFDGVPERFDQERVEKHGNSLVFKTSLFGNRVAVFCGPAGNKFLFGNENKLVSVWWPYSLRKLFGRCLVTSRGDEAKWIRKMMLSYLGPDAFATHYAATMDAATRRHIEVYWRGKEEVKVHQTIKLYAFELACRLFMSLEEPNHIAKLGSLFNIFLKGIIGIPIDFPGTRFHSSKKAASAIKAELLTLIKARRVEIEEGKESSSQDLLSHLLTSSDESGRHLTEMEIANNILILLFAGYDTSTITITLLMKRLGEHPDVYDKVLKEQLEISKAKKAGELLKWEDIQKMKYSWNVVCEVMRLNPPAIGAFREALVDFEYAGYTIPKGWKLYWSAAGTHKDEANFEDPTRFDPSRFEGSGPAPFTFVPFGGGPRMCLGKELSRLEVLTFLHNIITNFKWDLTIPDEKIRYAPMAIPEKGLPVRLHPHQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CT77</accession>
    <name>A0AAD5CT77_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Desiccation-related protein PCC13-62-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_032417</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007033">
        <property type="protein sequence ID" value="KAI7746135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CT77"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052965">
        <property type="entry name" value="Pigment-catalase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31694">
        <property type="entry name" value="DESICCATION-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31694:SF12">
        <property type="entry name" value="DESICCATION-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13668">
        <property type="entry name" value="Ferritin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7746135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="317" mass="34253" checksum="A5CDF5705FDD7EAC" modified="2024-05-29" version="1">MKIFNLFFRSPPSSTTTATVATIIFLFISTSVNSTANLPKTDMDLLEFPLNLEYFEAEYFLFGSLGKGLDTIEPGLAAKGPPPIGAKQANLSLLIKDVITQFAYQEIGHLRAIKKLVQGFPRPLLNLSAKSFGTVMDAAVGNPLSPPFDPYANDLNYLISCYVIPYVGLTGYVGANPKLESPDSRKLLYGYDKLVAGLLGVESGQDAVIRSLLYERANEKVVPYDITVGEFTQKISQLRNKLGNDGLKDEGLKVPIELGAEKNTQGNVLAGDEDSVAYGRTPQEILRIVYGTGDEKAPGGFYPQGGDGAIAKGYLNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CU97</accession>
    <name>A0AAD5CU97_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Leucine-rich repeat-containing N-terminal plant-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_032994</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RLP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006700">
        <property type="protein sequence ID" value="KAI7747727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CU97"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000041">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000213">
        <property type="entry name" value="Tyrosine-sulfated glycopeptide receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046956">
        <property type="entry name" value="RLP23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48061:SF12">
        <property type="entry name" value="DISEASE RESISTANCE LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48061">
        <property type="entry name" value="LEUCINE-RICH REPEAT RECEPTOR PROTEIN KINASE EMS1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 13" id="KW-0732">Signal</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042181008" description="Leucine-rich repeat-containing N-terminal plant-type domain-containing protein" evidence="13">
        <location>
            <begin position="24"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="834"/>
            <end position="856"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7747727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="869" mass="97763" checksum="661B2309E7BC1CE7" modified="2024-05-29" version="1">MAKSIHVLILLILVLLVLESALANKLSSLSHADECSHLYQFKESMSINKSASWDSRAYPKVASWNLNISYGARNCCLWDGVECKFGHVIGLDLSSSFLYGPISSNNSLFNLIHLRTLNLADNDFCSSQIPSGIGQLSQLANLNLSYSWFSGDVPKQISQLVSLISLDLSGYHNQLKLQSSDFQNLVQNSSETLRELLLSHVKIDSELPASIGNLTHLNILDLMSCGFTGTLPASISNLTQLTYLELCINKFTGSLPSLESFSNLTYLSLCYNNFDRWKLPDWFGKLKKITYLALENVNLYGEIPSSFFNLTHVEKLYLHKNQLKGQLSSALLNFQNLEEFYLAGVTVDFSLFLSLTKLKSLSLSGNNITFQVIDSRHTNETQHQFITLSLSYCNLKAFPEFLRFQHQLESLFLDNNNIEGLIPGWMWNISKETLERLSLSQNLLMGFEQHSPIAPWVRLQDLDLSYNMLHGSIPVPPPTTNWYSVSNNNLTGNIPPSICDLLSLWVLDLSFNNISGLIPPCLEKLSNSNLNVLNLSCNTLQGTIPNIFTTESKLQSIDLSRNKLEGKVPRSLEKCESLQILNLGYNFIDDIFPFWLGVLSKLQVLILKFNKFHGIIRIPAKFKLNFQKLHIIDLSHNLFSGDLPYQYFQEWSAMKETKANATYMQTNIGVPFTLQITNKGLTLVYVKIPYTFFVVDMPSNMFTGKIPESIQALTGLKVLNLSNNELSGVIPSSIGNLINLESLDLSRNKLSGMIPQDLVQLNSLAFFNVSNNSLTGRIPLGKQFNTFSNDSYIGNKALCGFPLSMHCGDSEESKPPTVSFKEDTESDFPNRIDWVVILIGVVTGLVIGIVCGDHLTTRYYMWFFQRFRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CWB7</accession>
    <name>A0AAD5CWB7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Haloacid dehalogenase-like hydrolase domain-containing protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_028244</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006396">
        <property type="protein sequence ID" value="KAI7749189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CWB7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd16415">
        <property type="entry name" value="HAD_dREG-2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.720">
        <property type="entry name" value="Haloacid dehalogenase-like hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011949">
        <property type="entry name" value="HAD-SF_hydro_IA_REG-2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044924">
        <property type="entry name" value="HAD-SF_hydro_IA_REG-2-like_cap"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02252">
        <property type="entry name" value="DREG-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01549">
        <property type="entry name" value="HAD-SF-IA-v1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46649">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46649:SF5">
        <property type="entry name" value="F14L17.7 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00413">
        <property type="entry name" value="HADHALOGNASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7749189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="260" mass="28576" checksum="718E1346F3E5E0DC" modified="2024-05-29" version="1">MDAGGLLRTLKPSNIIILKKPFTLRCSSSSNSPTTLHTASERPVKRAYDGLLLDAGGTLLQLSKPVADTYASIGRKYGLNATPADIKQGFKRAFSAPWPEKLRYQGDGRPFWKLVVSEATGCANNDYFEEVYEYYANGDAWHLPTGAYKTLLTLKESGVKLAVVSNFDTRLRKLLQDLNIIDVFDAVVISSEVGYEKPDAQIFETALDQISVEAAKAVHVGDDQTADKIGANAVGINCWLWGTEVKTFSDIQDRILVPEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CXB0</accession>
    <name>A0AAD5CXB0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Amidohydrolase-related domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_028222</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the metallo-dependent hydrolases superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006396">
        <property type="protein sequence ID" value="KAI7749167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CXB0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016831">
        <property type="term" value="F:carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000052">
        <property type="entry name" value="Catalytic/ hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032465">
        <property type="entry name" value="ACMSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21240">
        <property type="entry name" value="2-AMINO-3-CARBOXYLMUCONATE-6-SEMIALDEHYDE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21240:SF19">
        <property type="entry name" value="CATALYTIC_ HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04909">
        <property type="entry name" value="Amidohydro_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="1 2" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="3">
        <location>
            <begin position="53"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU366045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7749167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="337" mass="37400" checksum="F2E9E69CC4B95412" modified="2024-05-29" version="1">MAIVRINLTSLTPLLSSRTFLPSSRILFSSPPPVFRTDCTATNMTTTTTTKIIDSHLHVWASPQEAIDKFPYVPGQEPSIQGNFDFLLDCMEEAGVDGALIVQPINHKFDHSYVTSVLKKHPSKFVGCCLANPAEDGTGVQQLEHLILEDGYRAVRFNPYLWPSGQLMTNEIGKAMFAKAGELGVPVGFMCMKGLNLHISEIKKLCSEFPKTVVLLDHLAFCKPPMDDEEAKTFSELLGLSKFPQVYVKFSALFRVSRKPFPYEDLSPLLSQVVSSFGANRVMWGSDFPFVVEECGYKKAKEAVSAIANQIPISSSDLEWIMGKTAMQLFKGQWTSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CXP8</accession>
    <name>A0AAD5CXP8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_011008</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010006190">
        <property type="protein sequence ID" value="KAI7750181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CXP8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13999">
        <property type="entry name" value="STKc_MAP3K-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000476">
        <property type="entry name" value="PAS domain-containing protein tyrosine kinase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000060">
        <property type="entry name" value="Serine/threonine-protein kinase isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055164">
        <property type="entry name" value="EDR1/CTR1/ARMC3-like_pept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF96">
        <property type="entry name" value="OS04G0610900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14381">
        <property type="entry name" value="EDR1_CTR1_ARMC3_pept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="368"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="395"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="607"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7750181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="607" mass="67879" checksum="E6B28009BE0E469E" modified="2024-05-29" version="1" fragment="single">SATYFNRFYAPQRAHGERGVDVIDGGNGDDSRWSMVKGWCKSYYSQLTLAKRLTQEGTISAERALVEDSGCGGVGYDAESVSYRLWVNGTLSYDDKISDGFYDILGMDPYMWMMCNDSVEGKRLPSLLELKAVEAATSSMEVILVNRYKDSRLRDLEDIAQELYFSAETALMLAEKLAKLVAASMGGSFPVEQGDLHAQWETTSKRIKELQNNILVPIGSLSTGLCRHRAILFKKLADYVGLPCRIARGCKYCAEDHRSTCLIKIKDDKLAREYVVDLVGQPGNFYNPDSSINGDIVFHLEDIQVHVGHASISEVKSLHAPTGHALYTDVEVKSDGKNRGNLPVTTPTFLTPEPSLAMDWLEIPWDDLHIKERIGAGSFGTVYSAEWHGSVVAVKVLTAQDLHDNQLKEFLREVSIMRRVRHPNLVLFMGAVTMHKRFSIVTEYLPRGSLFRLLHHPASGEILNMKRRMHMALDVAKGVNYLHCLTPPIVHWDLKSPNLLVDKNWTVKVCDFGLSRFKTETFISSKAITGTPEWMAPEVLRGEPADEKSDVYSFGVILWELVTMQQPWKGLNAAQVVGAVAFQKRKLTIPPNASPALTSLMESCWAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CZ60</accession>
    <name>A0AAD5CZ60_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_029717</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006004">
        <property type="protein sequence ID" value="KAI7751058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CZ60"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45693:SF46">
        <property type="entry name" value="TRANSCRIPTION FACTOR TGA2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45693">
        <property type="entry name" value="TRANSCRIPTION FACTOR TGA9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BZIP" evidence="2">
        <location>
            <begin position="72"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS00036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7751058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="93" mass="10440" checksum="BEB63D0039877AEE" modified="2024-05-29" version="1">MRVTCIHKRRSIPTTLIICVNRGSIMAQLSPRTDTSTDGDTEDKAMGFHSSQSHGPVVSDASDKSRDQKTLRRLAQNREAARKSRLRKKVSFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CZ75</accession>
    <name>A0AAD5CZ75_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_028313</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006302">
        <property type="protein sequence ID" value="KAI7749704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CZ75"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13999">
        <property type="entry name" value="STKc_MAP3K-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF11">
        <property type="entry name" value="OS09G0443600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="167"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7749704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="444" mass="50680" checksum="921887A77EDC12AB" modified="2024-05-29" version="1">MIRQQQPKSIPVTANMSSTRQGNTSRSSQKRFCGAFGDCMTKPTIAASPPRHSSQRLEQQVEELHKEVKKEKDLKSVYKMRLERTQDYLKYCLQIAQDNGFLNLILNKKDDPQESSSASISSPSPSSVIIQASTDPHMPPVVQQNTDLSALIEQAKRYGWYIDPQEIELHKKIAQGSTAEIYQATWRGLEVAVKCIFPDYFKLNDCGVNFFAQEVETLSRQRHSYVLQLMGACLDPPNHGWLVTEFLNMTLKDWLHGPGKRKKERVIPLPPFEDRLAKALEIAQGMQYLHEHKPMIIHRDLKPSNIFLDDALHVRVADFGHARFLSDEEKAFTGETGTYVYMAPEVIRSEPYDEKCDVYSFGVILNELLTGQHPYIETNYGPSKIALEVGEGKIRPALLEHEENIEDLIELIQLSWDEDPEYRPSFATITRGLKLIYNRLTSSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-10-08" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D0N2</accession>
    <name>A0AAD5D0N2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">K Homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_027799</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005955">
        <property type="protein sequence ID" value="KAI7751187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009911">
        <property type="term" value="P:positive regulation of flower development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd22459">
        <property type="entry name" value="KH-I_PEPPER_rpt1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22460">
        <property type="entry name" value="KH-I_PEPPER_rpt2_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.210:FF:000002">
        <property type="entry name" value="KH domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10288">
        <property type="entry name" value="KH DOMAIN CONTAINING RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="7">
        <location>
            <begin position="32"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="7">
        <location>
            <begin position="128"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="7">
        <location>
            <begin position="256"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="7">
        <location>
            <begin position="341"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="426"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="8"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="428"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7751187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="449" mass="48686" checksum="23B3C0349FE2E7DC" modified="2024-05-29" version="1">MANQMNDYGKRDSNHQPDFPADAKESHGIGLEDTVYRYLCPSRKIGSIIGRGGEIVKQLRSETGAKIRISENLPGCEERVVIIYSSSDQTNSCGDVDEVVSPAQDALFKVHDRAIDEEAVGDEFDEMQQVVVRMLVPCDQIGCVIGKGGQVVQNIRNETRAQIRVLSGDYLPICALSFDELLQISGDAIAVRKALYQVASRLHDNPSRSQHLLFSSSSIHRGSGGYMGPNPGAPMGAYSLMGPYASYKNDSGNQAKEFSLRFVCPAENIGAVIGKGGVIIKQIRQESGAFVTVHSSAAEGDDCLITVSAKEVSIVLSKHGKIGGNAXXXCSERFEKDAGFFILTTRLLVPSSRIGCLIGKGGSIISEMRNLTRANIRILSIENENFPKVASEDEQVVQITGEINAASNALLQVTTRLRANVLEMDMPDVSRHNNRDGNYRGRGSQATTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D0T1</accession>
    <name>A0AAD5D0T1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="7">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_002867</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:20630"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:47005"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the PPP phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="7"/>
    <dbReference type="EMBL" id="JAMZMK010005932">
        <property type="protein sequence ID" value="KAI7751307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D0T1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07415">
        <property type="entry name" value="MPP_PP2A_PP4_PP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000005">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047129">
        <property type="entry name" value="PPA2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45619">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE PP2A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0464">Manganese</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="8">
        <location>
            <begin position="134"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7751307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="353" mass="40358" checksum="522E9E3C68CBE18B" modified="2024-05-29" version="1" fragment="single">GSRDKTEETKLHLPNPSKPLVPSAVVDSPIDLWISKVKEGNHLSEDELQLLCEYVKDILIEESNVQPVNSPVTVCGDIHGQFHDLMKLFQTGGHVPETNYIFMGDFVDRGYNSLEVFTILLLLKARYPANITLLRGNHESRQLTQVYGFYDECQRKYGNANAWRYCTDVFDYLTLSAIIDGTVLCVHGGLSPDVRTVDQIRVIERNCEIPHEGPFCDLMWSDPEDIETWAFNHINKLDLVCRAHQLVQEGLKYMFQDKGLVTVWSAPNYCYRCGNVASILSFSENMEREVKFFTETEENNQMRGPRTGREPEDSSLKLKFDSFRLVILNLEEFRVANQALQAGSIEELLDIPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D181</accession>
    <name>A0AAD5D181_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_013391</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005881">
        <property type="protein sequence ID" value="KAI7751532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D181"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24012">
        <property type="entry name" value="RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="80"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="287"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7751532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="428" mass="50275" checksum="792FC7B1F20AB850" modified="2024-05-29" version="1">MEQHINSSWSYDGPPPFNHGGGGGYNHHHRRFHNNNNNYHDFNDDSFGYSNGPAFSGRKRPFSHFSHPPPEYFDDDGRSNKLYVSSVPREVTEEDIHSLFGKHGTITEVVLFKQLKNLQQHGTICFFFIYDTNTVLLSFILNERKEKEKKRKRTKGNERKEGYVIVLQLCPHEKKLYVEYLFTNYSRAHHLEHRVEFGLMNCIFLLEFMHEYPHYNINELKSVYPDCCFVKYATIEDATQAIEALHNQYTFPGRLRPIEVRYATKKPERPGPGFLKTHENKFFGHENKVFVGSLNKYASKAEIAAIFSPYGFVENVFLNYDEQRQCRGTGFITFSDRAMAADAINGLNGKYVMKGCDQPLVVRFAEPKKPRVWENRYPPYFNDPWEKPEEYSFYEQQLENWNQQCNPTWGFLGLLLTKQLISPNYQFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D320</accession>
    <name>A0AAD5D320_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TF-B3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_020400</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005745">
        <property type="protein sequence ID" value="KAI7752152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D320"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd10017">
        <property type="entry name" value="B3_DNA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.330.10">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003340">
        <property type="entry name" value="B3_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015300">
        <property type="entry name" value="DNA-bd_pseudobarrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044837">
        <property type="entry name" value="REM16-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31391:SF106">
        <property type="entry name" value="B3 DOMAIN-CONTAINING PROTEIN OS01G0723500"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31391">
        <property type="entry name" value="B3 DOMAIN-CONTAINING PROTEIN OS11G0197600-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02362">
        <property type="entry name" value="B3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01019">
        <property type="entry name" value="B3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101936">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50863">
        <property type="entry name" value="B3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TF-B3" evidence="6">
        <location>
            <begin position="19"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="TF-B3" evidence="6">
        <location>
            <begin position="225"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7752152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="425" mass="47839" checksum="DABFF4B578FE2A63" modified="2024-05-29" version="1">MNARRPHFIKGFNPNISFDKLKIPSKFVKHLEGKTSGTVYLVGPSGNTWHADLAQETDGLFILNGWTAFVRDHFLENGDSLVFRYDDNLHFTLQIFDQSCCEKETAFSAECHQDLSIFDQHFGKKRDREYASWLSSTVDFVPKKPRSQPHEADNLHESVNEQCEAADFLNNVDFSSSGLETPITSAMPISEVSPNDDEIERLSASEADKIARSFTSSFPHFTQNVPYQFAMAYLPDRKVKIMLHNSKGESWTINSIPTTRVQTSHTFCGGWVAFVRSNNINVGDICIFELVGNCEMRVNILRVRQEPLDYDHGDVNGPVNGASKRISGRLTKTVKRKSFTTQSPALLEGQKVAFSIEKVKHGIAAKGSVDCHSRTTNKKSVMKNFNISGSYTLKIPVQFSMEHLPNCKTAITLRNLEGECWTVRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D3P1</accession>
    <name>A0AAD5D3P1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_021464</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the PP2C family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010005939">
        <property type="protein sequence ID" value="KAI7751265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D3P1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000291">
        <property type="entry name" value="Protein phosphatase 2C 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000222">
        <property type="entry name" value="PP2C_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01032">
        <property type="entry name" value="PPM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0464">Manganese</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="10">
        <location>
            <begin position="96"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7751265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="390" mass="42723" checksum="0BD6824F99F00280" modified="2024-05-29" version="1">MAEICYGVVNDKEASSPESSSRVARRKRMEIRKFKYVPTVGNDINGCKKPKLTVSPKSMSRGCYDTVDNICLTELKEDRGGGANAGGFVNNEPNPKFGVASVCGRRREMEDAVAIHPSFIEDNQKSHNLHYFGVYDGHGCSHVARRCKNRLHLLVREEYKNNDDWNNTMGQSFRRMDEEVTVWNQQLTTGDCRCELHAPESYGVGSTAVVAVVTPNKIIVANCGDSRAVLCRNGKAVPLSHDHKPDRPDELNRIESAGGKVLYWDGARVCGVLAMSRAIGDGYLKPYVISEPEVKITERTAEDECLILASDGLWDVVSNDTACGLASLCLKGNGPSAEMRSPPTNEVSEYENCDRACSNASLLLTKLALARGSADNVSVVVVDLRKNGSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D5D4</accession>
    <name>A0AAD5D5D4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Aminotransferase class I/classII large domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_009226</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005766">
        <property type="protein sequence ID" value="KAI7752045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D5D4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050596">
        <property type="entry name" value="AspAT/PAT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46383:SF5">
        <property type="entry name" value="AMINOTRANSFERASE CLASS I_CLASSII DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46383">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="6">
        <location>
            <begin position="33"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7752045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="190" mass="20895" checksum="510610C9F9CD2802" modified="2024-05-29" version="1" fragment="single">MGSYEMLARRAVNTVSPVMVQIQELVRGVKGAMSLAQGVVYWQPPRPALEKVQQLVWEPSISRYGADEGLPELREALTKKLREENNLCKSSVMVTAGANQAFVNIVLTLCDAGDSVVMFAPYYFNAYMSFQMTGVTDILVGPGDPNTLHPDADWLEKTLRETKPTPKLVTVVNPGNPSGTYIPEPLLKVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D5V0</accession>
    <name>A0AAD5D5V0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">HP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_029091</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the villin/gelsolin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005009">
        <property type="protein sequence ID" value="KAI7753959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D5V0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051693">
        <property type="term" value="P:actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051014">
        <property type="term" value="P:actin filament severing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11292">
        <property type="entry name" value="gelsolin_S3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11293">
        <property type="entry name" value="gelsolin_S4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11288">
        <property type="entry name" value="gelsolin_S5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000001">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.950.10">
        <property type="entry name" value="Villin headpiece domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007123">
        <property type="entry name" value="Gelsolin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036180">
        <property type="entry name" value="Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007122">
        <property type="entry name" value="Villin/Gelsolin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003128">
        <property type="entry name" value="Villin_headpiece"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036886">
        <property type="entry name" value="Villin_headpiece_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11977">
        <property type="entry name" value="VILLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11977:SF138">
        <property type="entry name" value="VILLIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00626">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02209">
        <property type="entry name" value="VHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00597">
        <property type="entry name" value="GELSOLIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00262">
        <property type="entry name" value="GEL"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00153">
        <property type="entry name" value="VHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82754">
        <property type="entry name" value="C-terminal, gelsolin-like domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47050">
        <property type="entry name" value="VHP, Villin headpiece domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51089">
        <property type="entry name" value="HP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0117">Actin capping</keyword>
    <keyword evidence="4" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="HP" evidence="6">
        <location>
            <begin position="829"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="674"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="736"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="789"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="704"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="755"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="797"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="894"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7753959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="894" mass="100018" checksum="9E5BD4BADAF0B81C" modified="2024-05-29" version="1" fragment="single">TNALKSGALRHDIHYWLGKDTSQDEAGAAAIKTIELDAALGGRAVQYREVQGHETERFLSYFKPCIIPQSGGVASGFNHAKPVEHKISMFVCQGKYVVHVPFARSSLNHDDIFILDTEHKIFQFNGSNSSIQERAKALEVLQYIRNAYHDGKCDIATIEDGRLMSDAETGEFWGFFGGFAPLPRKTQTDDALSTKALPTNKLFCVVKGQAEPVDAEPLTRELLDTNKCYILDCGVEIYVWLGRSTSLDERKAASGATEEYIHSQDRRKPQIICVIENFETVTFRSKFDSWPQATAVTVSEDGRGKVAALLKRQGVNVKGLLKAAPSKEEPQPYIDCSGNLQVWRVNGQEKILLPASDQSKFYTGDCYIYQYMYSGDDQDECLIGTWFGKQSIEEDKVSATSQANKMLESQKFLAAQLRVYEGKEPILFFAIFQSFMVLKSGLSDGYKKYILESELPNGTGIEEEVALFRVQGSGPENMQAIQLEPVASSLNSSYCYILHSGSSVFTWIGNLTTPEEQELVERQLDVIKPNMQSRLQKEGSESVEFWKMLGGKSEYPSQKTTMATESDPHLFSCTFSKGELKDDLMTEDIFILDCHSSVFVWVGQEVDPKIRTQALVIGEKFLERDFLLEKLPLQTPLYIIMEASEPQFFTRFFTWDSTKSAMHGNSFQRKLSILKNGGRPTSNNKPKRRTHVSNGGRSVAVEQPQRSRSVTFSSDRVRVRGRSPAFAALASTFENPNARHLSTPPPLENKIYPKSNGSDSSKPASRSTAIASLTATFEPPRAKFIPRSIKVMPEPPAKSESKSNGDSKGNRIETPTIHEDVKAGETADEEGLALYPYERLTVSSTNPAPDINIFKRETYLSSAEFRAKFGMAKSAFYKLPKWKQNKLKMALHLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D6R9</accession>
    <name>A0AAD5D6R9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_006756</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010002278">
        <property type="protein sequence ID" value="KAI7754908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D6R9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35717">
        <property type="entry name" value="OS05G0156200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35717:SF1">
        <property type="entry name" value="OS05G0156200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="122"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="15"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="127"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7754908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="259" mass="28089" checksum="7DFB4D6300A7684B" modified="2024-05-29" version="1">MRVEGGVSETNPTTPSESESLHVSASAASVTASPSSSTVASPSGKRSRDPEDEVYVDNLHSHKRYLSEIMASSLNGLSVGDPLPENLMDSPARSDSMFYLRDELPLQYSPMSEDLDECRYYESNACSPSPSQPDSQPTSPVSPYRFQRLAAATTFSPGPVYPGAPAVTPSQARQRGTEPEGRFPSSPSDICHSADLRRAALMRSVQMRTQPMGSSQLDAGFSPMQEVVQEVDSECPIHEKSCRVLSMNMNMKGDDETTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D6W1</accession>
    <name>A0AAD5D6W1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_017997</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010002199">
        <property type="protein sequence ID" value="KAI7754968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D6W1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35699">
        <property type="entry name" value="F2J10.10 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35699:SF1">
        <property type="entry name" value="F2J10.10 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="129"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7754968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="197" mass="22416" checksum="A6F37A68521BDDF0" modified="2024-05-29" version="1">MASIQILKSGLHPFSSLQSSSHTNKAFICYKSNIRINRRFRVSCKGQDSDDNDIKGEEPTESLFMKELKRRGISRTSLLEKDENVGYKEEDGGFSNTYADIEENLSNQRERSMSLNSEGLEGLIPRGKVLLTLGGTFFLAFWPLILVTVASFSAVYLYFGPKFVHDATTRQVYLPRYVDPYELLEDQRISETAPRLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D6Z2</accession>
    <name>A0AAD5D6Z2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">HTH myb-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_011788</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010003272">
        <property type="protein sequence ID" value="KAI7754484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D6Z2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000007">
        <property type="entry name" value="Two-component response regulator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044825">
        <property type="entry name" value="GLK1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006447">
        <property type="entry name" value="Myb_dom_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01557">
        <property type="entry name" value="myb_SHAQKYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31312">
        <property type="entry name" value="TRANSCRIPTION ACTIVATOR GLK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31312:SF1">
        <property type="entry name" value="TRANSCRIPTION ACTIVATOR GLK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="7">
        <location>
            <begin position="138"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="100"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="281"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="284"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7754484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="419" mass="45719" checksum="B4E57A62A7C5D110" modified="2024-05-29" version="1">MLAIVSPLGTNTGRDGRDQGCDPVGFSVGNEFPDFSDDNLLESIDFDDLFVGMDDQDMLPGLEMDPELLTGEFSISGGDDSCEYNNNSVTSFSDDKKIDDFTSRKEAPGSNLVPKQKETNVRGVKSLSDKSSKNSAQGKRKVKVDWTPELHRRFVQAVEQLGVDKAVPSRILEIMGIGCLTRHNIASHLQKYRSHRKHLLAREAEAASWSHRRQMYGGTATKSVGGVGSAGGKRDMGPWVAPPPSMGFPSMTPVPHYRPLHVWGHPSMDRPLMPVWPKHIAHSPSPPPPPPSPSPHLWPPSTSPPPTWHPGHQRAPYFPQILPQTRFPIPHVAVPGVPPPHAMYKVDSGIGTTSPSTGQSCPRPPLDLHPTKESIDAAIGDILSKPWQPLPLGLKPPALDSVMGELHRQGIQRIPPSCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D872</accession>
    <name>A0AAD5D872_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Dof zinc finger protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_022812</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Transcription factor that binds specifically to a 5'-AA[AG]G-3' consensus core sequence.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005438">
        <property type="protein sequence ID" value="KAI7753425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D872"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045174">
        <property type="entry name" value="Dof"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003851">
        <property type="entry name" value="Znf_Dof"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31992:SF350">
        <property type="entry name" value="DOF ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31992">
        <property type="entry name" value="DOF ZINC FINGER PROTEIN DOF1.4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02701">
        <property type="entry name" value="Zn_ribbon_Dof"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01361">
        <property type="entry name" value="ZF_DOF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50884">
        <property type="entry name" value="ZF_DOF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 8 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3 9" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 8 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Dof-type" evidence="11">
        <location>
            <begin position="50"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="16"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="91"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="19"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="105"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU369094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7753425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="298" mass="32498" checksum="35CAA5263EE102BA" modified="2024-05-29" version="1">MYEIVMNPMEEILVPYTTNSSSSDNNNNNNLSKPSSSFERRVRPQKAAAVNCPRCDSTNTKFCYYNNYSLSQPRYFCKSCRRYWTEGGTLRNIPVGGGSRKNKRSSSSSSSTTSSSSYSSKLPDLVIPTLSTAVRSQNPRISHDQYGQDLNLGFISSTNNFKNVPEFFQVPNFDATRNSSTCGSLVHRVCPTESAQFSSLEPLIGVATNSFVPISNSVNTPLGELMIPIPEFKIPSLGFSLDGMGSGVGGDGRILFPLEEVKTGTTSTSHDGRQSTKDVQNCDSNGFWNGMMGSGGSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DD17</accession>
    <name>A0AAD5DD17_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_031048</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000366">
        <property type="protein sequence ID" value="KAI7756486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DD17"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7756486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="154" mass="17699" checksum="7A79DC2BC316F9CD" modified="2024-05-29" version="1" fragment="single">MLTAYIDLLQGLRKESGHHIEPMALSGPRIYDNKLLLYTPQRFYFLEVLQILQQASLLTHAEPLTPHYLLVEDVVVLRWFISHLEQPLEVPESNSHKVMFGRDAYIVRKMGGRVDMESTNVSGIRNEFKREGAGPGNWAFERFVVTVRVAATFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DE25</accession>
    <name>A0AAD5DE25_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Pectate lyase</fullName>
            <ecNumber evidence="3 8">4.2.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_032084</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>Eliminative cleavage of (1-&gt;4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.</text>
            <dbReference type="EC" id="4.2.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="8">Binds 1 Ca(2+) ion. Required for its activity.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 2/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the polysaccharide lyase 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.2.2" evidence="3 8"/>
    <dbReference type="EMBL" id="JAMZMK010000085">
        <property type="protein sequence ID" value="KAI7757680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DE25"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030570">
        <property type="term" value="F:pectate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018082">
        <property type="entry name" value="AmbAllergen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002022">
        <property type="entry name" value="Pec_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045032">
        <property type="entry name" value="PEL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31683:SF136">
        <property type="entry name" value="PECTATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31683">
        <property type="entry name" value="PECTATE LYASE 18-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00544">
        <property type="entry name" value="Pectate_lyase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00807">
        <property type="entry name" value="AMBALLERGEN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00656">
        <property type="entry name" value="Amb_all"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0106">Calcium</keyword>
    <keyword evidence="7 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041778998" description="Pectate lyase" evidence="8">
        <location>
            <begin position="22"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="domain" description="Pectate lyase" evidence="9">
        <location>
            <begin position="150"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7757680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="569" mass="60802" checksum="9500E3E65A781CF2" modified="2024-05-29" version="1">MSPCACTRLLTYMLYLTCISSTIINTTLPHQHPDPEAIVMQLQRKVNASIASRRRLLETNPLYIQSKDQTPCQTGNPVDDCWRCDPNWANDRQHLADCAIGFGQGAMGGKGGQIYVVSDPSDGDPENPQPGTLRHAVIQSEPLWIIFAHDMHINLKTQLIVSSSKTIDGRGAIVHITGKGCIVIENVGNIIIHGLYIHDCEPSGKAKIRVSPTDVVGRGKSDGDGLTVRGVRNLWIDHCSFARCTDGLVDITEGSTAVTVTNSYFTEHDKVMLLGHSDDFLADAGMQVTVAFNHFGKGLVERMPRCRHGYFHVVNNDYTEWQLYAIGGSAAPTINSQGNRFVAQPNSHKEVTKRMEAKEDEWRGWNWRTEGDLMVNGAFFVPSGPEFSVQYDKASSIPAMSAYLINQLTMHAGVLVGVPSNVGDNPPPGIIPPGSGGGPMIMPPVDGQPNGGNPPGTIPPYGGDYGYSPGTIPPGGNRYGATPPGCYAPGKTQGGCIPGMVSGGGYSSVQGSPPFGVLQGCGPGIIPYCGGPDGRRVSGVSSNIYKMARSSVPINILMSSLVVLSSVLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DE33</accession>
    <name>A0AAD5DE33_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_029180</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000057">
        <property type="protein sequence ID" value="KAI7757804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DE33"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006502">
        <property type="entry name" value="PDDEXK-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01615">
        <property type="entry name" value="A_thal_3542"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31579">
        <property type="entry name" value="OS03G0796600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31579:SF1">
        <property type="entry name" value="OS03G0796600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04720">
        <property type="entry name" value="PDDEXK_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="287"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="338"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="287"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7757804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="384" mass="43007" checksum="62D5C388A09747C3" modified="2024-05-29" version="1">MHFQMKIQPIDNNSYDESVNSESPATVKPPVLKSRFKRLFDRQFPSVLKINTDKVNVSGEVKDAEFEPSSVCLAKMVQNFIEDAAPEKPKCGRNRCNCFNGNINDVSDDEFDLSAAGCFGETLKSLTPCVSVIQRNLLADTSKIVEKNKTCKRKDELIKLVAEELLLIGYDASICKSHWEKSSSYPAGEYEYLDVIFEDSERVLIDIDFRSEFEIARPTGNYKAILQSLPYIFVGEADRLQQILSIVTEAAKLSLKKKGMHVPPWRKFEYMRSKWLSPHIRTPLPSPLTLPSPLPLTPPTPPPTPDANAASKQVFARTAVEDKEPATECGVFEQETTAFQSKSDEGFETPTVGKWEPPAIKPRNVERGSKLVVTGLASLFREKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G2L6</accession>
    <name>A0AAD5G2L6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_031774</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011852">
        <property type="protein sequence ID" value="KAI7725817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G2L6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.20">
        <property type="entry name" value="Apolipoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34360">
        <property type="entry name" value="OS08G0519400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34360:SF1">
        <property type="entry name" value="OS08G0519400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58113">
        <property type="entry name" value="Apolipoprotein A-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042147696" evidence="2">
        <location>
            <begin position="26"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="33"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="104"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7725817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="351" mass="40324" checksum="B98FE7211B765258" modified="2024-05-29" version="1">MASFNLHTFFLLSLSFALIFTFSHADTAIQQPVDAFRSELDKLTSKIHSLELLVEEKNHELKNKDEMIAQKEKVIAEKEKIIIEKSDSISSLKNDVASLEVKASLDANEQLKKAHTRVHELEKQVDELQTEIKLKENLREGLETRIKELEEKTVDLNSKLNDLQQVADERKRKLRKTERALKIAEEELMKAKFEATSKIKELTEVHGAWLPPWLAARLAGFQVFEKKAQAEKWAEPHVQTIKTKWIPAAREQWVLVVTNVEPQLQSLTKKTKEVYIQSKDALTPHVIKIKDVVDPHFQTVKKVSKPYIDQIATATKPHLDKARETVAPYTKEAVIAYGKFLESATKYHHQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G3R1</accession>
    <name>A0AAD5G3R1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">M8C21_019334</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">AA19_3_7</strain>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011345">
        <property type="protein sequence ID" value="KAI7727585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G3R1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000101">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase ERECTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000282">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase FEI 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046959">
        <property type="entry name" value="PRK1-6/SRF4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48007">
        <property type="entry name" value="LEUCINE-RICH REPEAT RECEPTOR-LIKE PROTEIN KINASE PXC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48007:SF76">
        <property type="entry name" value="OS03G0145102 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="39"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="295"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="362"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="394"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAI7727585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="565" mass="62085" checksum="D3B8FA4EBDEED739" modified="2024-05-29" version="1">MNSVGILEVFSSITPCPKLLTFADFIFNIHQFFDTFRKAVIFLLLCFSSLVSTSITAMGIFFTTFQGPLLVYCLLLFCLVNQLHCLTSDGEALVNFRTSIISSDGLLRQWRPEDPDPCGWKGVTCDMKTKRVVYLNLSNHKLSGPVSPDIGKLDRLKFLDLHYNNFYGAIPPELGNCTELQGLFLQNNYFSGSIPTEIGNLSKLQTLDVSSNSLSGSIPTSLVYLNSLSIFNVSNNFLAGPIPSDGAFDKFEANSFLGNRGLCGTHISQSCKNDGGSPNSHPTGSQSVKKNSGRLLISASATVGALLLVALMCFWGCFLYKKFGKNDVKGLAVDVSGGASIVMFHGDLPYSSKDILKKLETLNEEHVIGAGGFGTVYKLAMDDGNIFALKRIVKLNEGFDRFFESELQILGSIKHRYLVNLRGYCNSPTSKLLLYDYLSGGSLDEALHEYMQSGRATEKTDIYSFGVLVLEVISGKRPTDASYIEKGLNIVGWLNYLITEDRQREIIDVNCEGVESATLDAVLSLAIQCVSSSPEERPTMHRVVQTLESEIMTPCPSDFYDSSSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G536</accession>
    <name>A0AAD5G536_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">PGG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_019083</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011166">
        <property type="protein sequence ID" value="KAI7728712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G536"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000217">
        <property type="entry name" value="Ankyrin repeat-containing protein ITN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000245">
        <property type="entry name" value="Ankyrin repeat-containing protein ITN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026961">
        <property type="entry name" value="PGG_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24186:SF49">
        <property type="entry name" value="ANKYRIN REPEAT FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24186">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13962">
        <property type="entry name" value="PGG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 9" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0346">Stress response</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="repeat" description="ANK" evidence="9">
        <location>
            <begin position="211"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="9">
        <location>
            <begin position="279"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="9">
        <location>
            <begin position="347"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="PGG" evidence="11">
        <location>
            <begin position="461"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="17"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF13962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7728712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="541" mass="58881" checksum="C17699417B4EBD55" modified="2024-05-29" version="1">MKTVFEGEERDLEMGSDPSSNPIHQRSPSPRRVIDPFPAPSPRAPALVVSNSGKFSVSSSNAAKFYGASSSNSLLNASSSSKSLPYTSSNSGKSLLGSCSGKSLMSQGGKKKYVQQVTGKHNDTELHLAAKHGDVMEVRRILDEINEQMLRTMKGADFDDDVAEIRASVVNEMNELGETALYTAAERGFIEVVKELLPYTTKEGLALKNISEFDPLHIAAREGHREIVKILLDHDPGLSKTVGQWNSTPLTTAAARGHSSVVNELLSQDSTLVDITRSNGKNALHLAARQGHVDIVNALLKKDPQLARRTDKKGQTALHMAVKGVCCEVVRLLLHADPAIVMLPDKFSKTALHVATRKKRVEIVNELLLLRDTNVNALTRDHKTALDIVDNLPLSEETSEIKEILTRYGARKANDLNQPKDELRQTVSEIQKDVHDQVEQARRTNRNMSGIAKELRKLHREGINNATNSVTVVAVLFATVAFAAIFTVPGGDYVDGTAVMAHAPSFQVCYVVYVVNDGGCGGDKNSIKVLKYKHNGGEGGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G599</accession>
    <name>A0AAD5G599_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_030479</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011126">
        <property type="protein sequence ID" value="KAI7728877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G599"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45667">
        <property type="entry name" value="S-ADENOSYLMETHIONINE MITOCHONDRIAL CARRIER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="352"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="8">
        <location>
            <begin position="8"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7728877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="378" mass="41260" checksum="5D917A4D8CA9AEF0" modified="2024-05-29" version="1">MEDFIKSQTFAIHAAAGAGAITLGTATTYPFETIKTLIQVGSGPDKQLSAAQVFDRVRKLSGSFGLYNGFGWLAMGRMLGVGARFGTYELLTAFYKDGRKDNYVYVSEALIAGIVAGGVESLMSSPFELFTVRAQVAAASRAPNSSTNLQKPSVSSPAANLLRGYHPDVKTLDHSVGLLSTLNTKQSNLTGSLKEYPWMMTGTGRAPPVYHVRRPIDVISLEGWGAMWRGIRSGLVRDCIFGGVFFSSWQFLHRLMLDWKAVGMNPIPSSDEDVGPLHPLAVSLAAGFSGSIAAAASHSFDTAKCRSQCLVLPKYITMERKFLRWSLPGKRFERLTGIHPRDRNILFRGIKLRMACSGVASFILTGSYFFIVDHLVSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G5A5</accession>
    <name>A0AAD5G5A5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_007782</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011013">
        <property type="protein sequence ID" value="KAI7729370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G5A5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7729370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="179" mass="20160" checksum="09CA224589E85BDC" modified="2024-05-29" version="1">MSVEMFLPCSRILLCSQVDDPKNEAALNVRCKKSPKNLEIQLLSQFKITTFHLNTTVLQLMTCLEPNMNSQNHWQEKRNINHLLLEGIVSICRKNNNGPAAVRNLQLGSTNETTTQQLQTCKYMSDKYSQLPRSVTVGQSKPILPIAKMKMKPAPTRTTATRLGTTSSFEAMISPVYTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G5I4</accession>
    <name>A0AAD5G5I4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">(+)-abscisic acid 8'-hydroxylase</fullName>
            <ecNumber evidence="14">1.14.14.137</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">M8C21_019440</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">AA19_3_7</strain>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>2-cis-(+)-abscisate + reduced [NADPH--hemoprotein reductase] + O2 = (+)-8'-hydroxyabscisate + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12897"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:37569"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:58490"/>
            <dbReference type="EC" id="1.14.14.137"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 15">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="13">Plant hormone degradation; abscisic acid degradation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.14.137" evidence="14"/>
    <dbReference type="EMBL" id="JAMZMK010011071">
        <property type="protein sequence ID" value="KAI7729102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G5I4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010295">
        <property type="term" value="F:(+)-abscisic acid 8'-hydroxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046345">
        <property type="term" value="P:abscisic acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009737">
        <property type="term" value="P:response to abscisic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009414">
        <property type="term" value="P:response to water deprivation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016125">
        <property type="term" value="P:sterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11043">
        <property type="entry name" value="CYP90-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000014">
        <property type="entry name" value="Abscisic acid 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24286:SF375">
        <property type="entry name" value="ABSCISIC ACID 8'-HYDROXYLASE 4-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24286">
        <property type="entry name" value="CYTOCHROME P450 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 15 16" id="KW-0349">Heme</keyword>
    <keyword evidence="9 15 16" id="KW-0408">Iron</keyword>
    <keyword evidence="11 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 15 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 16" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="8 16" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="15">
        <location>
            <position position="417"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00050609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00060633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00066338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAI7729102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="469" mass="53713" checksum="63691F904694CF83" modified="2024-05-29" version="1">MTIFAIIFYSAIFLSSLFSYYMFKKKKRRHKTRARLPPGSMGLPCIGETLQMYTQDPNVFFASKQKRYGDIFKTRILGYPCVMLASPEAARFVLVSHAHLFKPTYPKSKEKLIGPSALFFHTGNYHACMRKLVQTSFSPEVTRKLIPDIERLAISSLESWDNGQVINTFDAMKKFAFEVGILSIFGHLNKKYTDLLKKNYSILEKGYNCFPLKLPGTAYHKSLMARKRLNQILGKIVRERKENKLSANNLLGYLLNFRDEKGNTLSEETIADNIIGVLFAAQDTTASVLTWILKYLSDDPKLLVAVKGEQKAICESEFGENRSLTWAMTRNMPLTYRVILESLRKASVISFTFREAAVDVEYEGYLIPKGWKVMPLFRNIHHNPEFFADPGNFDPFRFENPPKPFTYMPFGSGMHACPGNELAKLEMLVLLHHLLTKYRWELVGSANLIQYNPFPIPENGLGARFTKEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G5J4</accession>
    <name>A0AAD5G5J4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 13">Hypoxanthine phosphoribosyltransferase</fullName>
            <ecNumber evidence="5 13">2.4.2.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">M8C21_033181</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">AA19_3_7</strain>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate</text>
            <dbReference type="Rhea" id="RHEA:17973"/>
            <dbReference type="ChEBI" id="CHEBI:17368"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="EC" id="2.4.2.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 13">Purine metabolism; IMP biosynthesis via salvage pathway; IMP from hypoxanthine: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the purine/pyrimidine phosphoribosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.8" evidence="5 13"/>
    <dbReference type="EMBL" id="JAMZMK010011062">
        <property type="protein sequence ID" value="KAI7729132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G5J4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004422">
        <property type="term" value="F:hypoxanthine phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032263">
        <property type="term" value="P:GMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006178">
        <property type="term" value="P:guanine salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046100">
        <property type="term" value="P:hypoxanthine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032264">
        <property type="term" value="P:IMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000057">
        <property type="entry name" value="Hypoxanthine phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050408">
        <property type="entry name" value="HGPRT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005904">
        <property type="entry name" value="Hxn_phspho_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01203">
        <property type="entry name" value="HGPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43340:SF1">
        <property type="entry name" value="HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43340">
        <property type="entry name" value="HYPOXANTHINE-GUANINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 13" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="12 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10 13" id="KW-0660">Purine salvage</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="14">
        <location>
            <begin position="18"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU364099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAI7729132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="193" mass="21211" checksum="D048DD9B1CF2EA08" modified="2024-05-29" version="1">MSSRRCIDDDILTVLWTQDQLSHKVEDIASQITHDFSSSSSSCCPVIVGVATGAFLFLADLVRNIKLPVTVDFVRAESYGSGTVSTGVPKLSCDVKLDVTGKHVVLVEDIVDTGNTVSRLISYLEAKGATSVSVCTLLDKPARRKVHFELLGDGKFYSGFECPDYFVVGYGLDFNEQYRNLPYIGVLKPEIYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G722</accession>
    <name>A0AAD5G722_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF7054 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_026127</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010927">
        <property type="protein sequence ID" value="KAI7729748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G722"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040358">
        <property type="entry name" value="At4g22758-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055482">
        <property type="entry name" value="DUF7054"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33270:SF36">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33270">
        <property type="entry name" value="BNAC05G50380D PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23156">
        <property type="entry name" value="DUF7054"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7054" evidence="1">
        <location>
            <begin position="19"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF23156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7729748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="149" mass="16677" checksum="AF4EF888434205FD" modified="2024-05-29" version="1">MMIKNKMKERERLTMTRPKLTKLLLNVTVERSLVPVHVIISPESTVGDLIAAALRQYSKEGRWPVIPLVNYHGFDLHYSQFCLESLSRDEKLNELGSRNFFLCSNQSVTVSGSNGSGMTASPSSSTCSKDTGEVMRGGTVLLRFMEFML</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G7V6</accession>
    <name>A0AAD5G7V6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rhodanese domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_016341</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010248">
        <property type="protein sequence ID" value="KAI7732385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G7V6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00158">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044664">
        <property type="entry name" value="STR11-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45187">
        <property type="entry name" value="RHODANESE-LIKE DOMAIN-CONTAINING PROTEIN 11, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45187:SF2">
        <property type="entry name" value="RHODANESE-LIKE DOMAIN-CONTAINING PROTEIN 11, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="1">
        <location>
            <begin position="101"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7732385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="292" mass="32392" checksum="515C6D189D263560" modified="2024-05-29" version="1">MEATVLSISPLISTPCNPNNNPHSNTHRLNKTHLFNSLSFTHKPLIFQSTYKGVVKAQAVDEDYELKQVKDMAAARKRWDALVREGKVKVLTPKEAGYAIQLSGKTLLDVRPSSEHEKAWVKGSTWIPIFDVDTQLDAGTLSRKVTSYMMGGWWSGVPTLSFDNRFISKVTEKFPEDADLIVACQKGLRSLAACELLYNAGYRNLFWVQGGLEAAEEEDLEREGPQPFKLAGVGGLSGFLGWTDQQRAQAAKEGWGYRLVFTGRLIGVVLAADALFLAVQQAARYLQDIRGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G8N3</accession>
    <name>A0AAD5G8N3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_024173</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010205">
        <property type="protein sequence ID" value="KAI7732567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G8N3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7732567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="82" mass="9338" checksum="B6A08511050955BB" modified="2024-05-29" version="1" fragment="single">NIIAKNSGTKIDVTIKKIRNADLKEIKLLYYMDNENLLDDTDLQQILPRGLKPSNLLLNANCDLKICDFGLARTSETHFMTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GA63</accession>
    <name>A0AAD5GA63_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_013523</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Functions as an E3 ubiquitin ligase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="JAMZMK010010162">
        <property type="protein sequence ID" value="KAI7732763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GA63"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16664">
        <property type="entry name" value="RING-Ubox_PUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000082">
        <property type="entry name" value="RING-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058678">
        <property type="entry name" value="ARM_PUB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057623">
        <property type="entry name" value="PUB12-19-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045210">
        <property type="entry name" value="RING-Ubox_PUB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003613">
        <property type="entry name" value="Ubox_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23315">
        <property type="entry name" value="U BOX DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23315:SF52">
        <property type="entry name" value="U-BOX DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25598">
        <property type="entry name" value="ARM_PUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25368">
        <property type="entry name" value="PUB10_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04564">
        <property type="entry name" value="U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00504">
        <property type="entry name" value="Ubox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51698">
        <property type="entry name" value="U_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="U-box" evidence="9">
        <location>
            <begin position="169"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="8">
        <location>
            <begin position="262"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="8">
        <location>
            <begin position="303"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="8">
        <location>
            <begin position="344"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7732763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="494" mass="54435" checksum="97D2C907F346AA1D" modified="2024-05-29" version="1" fragment="single">MSRRVVFSSSSCLSDLMAALQAVRKLLISSANFDHKLSPDIGSAKKISLQFQSVTFKLEQALANLPYDSFDISEEVKEQVDLVRDQMRRARERYGKPLNSAADKDLLHSAETTINIIHQDSKTKAESASKEPVTNAYNQNEAVYQLETLKSSSETSCLLDENKKLDAPAIPVDFLCPISLELMRDPVIVSTGQDLSVDTYERSYIQRWIDCGNTTCPKTQQKLQNIILTPNYIYKQLKFSFETYQARQSKNEDNRILLAEAGAIPVLVSLLTSEDNFTQENAVTSLLNLSIYENNKELIMLANAVPSVVQLLRTGTMETRENAAAALFSLSLANENKIIIGASGAIPELVNLLGNGSNRGKKDAATALFNLCVYHGNKGRAVRAGIIHVLLKMLSDSSMVDESLTILSILASYKEAKTAVVKASIIPVLIDLTRTGPPRSKENATSVLLSLCRRDKDNLECISRLGAVIPLTELAVNGSERAKRKAKSLLEHLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GAK0</accession>
    <name>A0AAD5GAK0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Proteasome subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_017863</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Component of the proteasome, a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="function">
        <text evidence="2">The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleavage of peptide bonds with very broad specificity.</text>
            <dbReference type="EC" id="3.4.25.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the 20S core complex of the 26S proteasome. The 26S proteasome is composed of a core protease (CP), known as the 20S proteasome, capped at one or both ends by the 19S regulatory particle (RP/PA700). The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the proteasome complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the peptidase T1B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009476">
        <property type="protein sequence ID" value="KAI7735505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GAK0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005839">
        <property type="term" value="C:proteasome core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004298">
        <property type="term" value="F:threonine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03763">
        <property type="entry name" value="proteasome_beta_type_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000005">
        <property type="entry name" value="Proteasome subunit beta type-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000243">
        <property type="entry name" value="Pept_T1A_subB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF4">
        <property type="entry name" value="PROTEASOME SUBUNIT BETA TYPE-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00141">
        <property type="entry name" value="PROTEASOME"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="8 11" id="KW-0647">Proteasome</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0888">Threonine protease</keyword>
    <feature type="active site" description="Nucleophile" evidence="10">
        <location>
            <position position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR600243-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7735505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="273" mass="29523" checksum="1D08C059CFE290A2" modified="2024-05-29" version="1">MSTEAMDVPKTGFSFDLCRRNDMLTKKGLKSPGYLKTGTTIVGLIFENGVILGADTRATEGPIVADKNCEKIHYMAPNIYCCGAGTAADTEAVTDNISSQLKLHRYHTGRESRVVTALTLLKSHLFRYQGHVSAALVLGGVDVTGPHLHTIYPHGSTDTLPFATMGSGSLAAMAIFESEYREGLTRDEGVNLVTKAICSGIFNDLGSGSNVDVCVIEKGKKEYLRNHLTPNPRTYISSKGYTFSKKTEVLLTKITPLKELVDVVQVGGDAMEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GB88</accession>
    <name>A0AAD5GB88_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Potassium transporter 11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_018235</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HAK/KUP transporter (TC 2.A.72.3) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009838">
        <property type="protein sequence ID" value="KAI7734096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GB88"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015079">
        <property type="term" value="F:potassium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003855">
        <property type="entry name" value="K+_transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053952">
        <property type="entry name" value="K_trans_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053951">
        <property type="entry name" value="K_trans_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30540">
        <property type="entry name" value="OSMOTIC STRESS POTASSIUM TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30540:SF95">
        <property type="entry name" value="POTASSIUM TRANSPORTER 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02705">
        <property type="entry name" value="K_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22776">
        <property type="entry name" value="K_trans_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0630">Potassium</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="64"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="112"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="146"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="174"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="202"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="239"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="279"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="K+ potassium transporter integral membrane" evidence="11">
        <location>
            <begin position="7"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="K+ potassium transporter C-terminal" evidence="12">
        <location>
            <begin position="253"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF22776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7734096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="495" mass="55715" checksum="512B20BF948DA1C8" modified="2024-05-29" version="1">MLNDLRTEALFADLAHFPVSAIQLAFTVVVFPCLLLAYSGQAAYLMNNKDHVSDAFYRSIPDGIYWPMFIIATLAAIVASQATISATFSIIKQANALGCFPRVKVIHTSSKYLGQIYIPDINWILMILCIVVTAGFKKQSQIGNAYGTAVVIVMLATTFLMILIMLLVWRCHWILVFIFAALSLIVELTYFSAVLFKIDQGGWVPLVIAAVFLFIMYVWHYGTVKRYEFEMHSKISMAWILGLGPSLGLVRVPGIGLVYTELASGVPHIFSHFITNLPAIHSVVVFVCVKYLPVYTVPEDERFLVKRIGPKNFHMFRCVARYGYKDLHKKDEEFEKKLFDNLFTFVRLESMMEGGSDSDDYSVYGQQTQRSKDFLLSDNNNNTFSSVVDLTISSCDSIVPATKSPLHALNSGTSSGQVSSQRDVDEMDFLTTCRDAGVVHIMGNTVVKARRDSPFYKKLSIDYVYAFLRKICREHSVIFNVPHESLLNVGQVFYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GC25</accession>
    <name>A0AAD5GC25_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_027251</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009151">
        <property type="protein sequence ID" value="KAI7736975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GC25"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043561">
        <property type="entry name" value="LHW-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025610">
        <property type="entry name" value="MYC/MYB_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46196:SF15">
        <property type="entry name" value="MYC-TYPE, BASIC HELIX-LOOP-HELIX (BHLH) DOMAIN, TRANSCRIPTION FACTOR MYC_MYB N-TERMINAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46196">
        <property type="entry name" value="TRANSCRIPTION FACTOR BHLH155-LIKE ISOFORM X1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14215">
        <property type="entry name" value="bHLH-MYC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23176">
        <property type="entry name" value="bHLH_LHW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="6">
        <location>
            <begin position="437"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="410"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="435"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7736975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="681" mass="75778" checksum="07AE898776959739" modified="2024-05-29" version="1">MATTQRVSTVTNVIKNLCRSYGWSYAVLWSFDQSNSMLLTMQDAYFEDHIQGLVDDLLSQSSMFGGGLIGQAALTKRHKWISSKDNYIGQSSSGSIWDMFKDDSKLFRQFSSGIETIAAIPVEPQGVVQFGSTNKVVENIEFINHTKRIFNDIVNGNAPTSSSSSPNGLLSSLMSSEDHYSINQTCFGFPQQSFASGLHSQSMFLDHSRSTSLTSFKEPPLHDMFDLPMDLGSMDELFQTGDFNISQWYPQSPIKSNVTINDHLLSQATGFFPISSDEQVNPLSISGVDVNLFGNTGDLGDIVTPVLNGNHLGYDSYNSGRTLASQTTQSIDNDLATFPPKKGLFSNLGIKELFEGVSSTSTSCVDDQVSSCSKRRKTGNPILEMGSLQPAVYNKSFGLKSEPLFNSDRWSGDGYSNDGSSTILQAKKPVEPTKPKKKAKPGTRPRPKDRQMILDRMAELRELIPNGEKMSIDCLLDRTIKHMLFLQSVTEHADRIKQVDEPKHYGVTKNDYLKDPSNNGVTVACELGSQSMICPLIVEDLSTPGQMLIEMLCEEHGFFLEIVDIIRGFGLTILKGVMEAREEKIWARFIVEAEAKRHVTRHEIFAALVQLLQTIGSNDNHFDKNIMQTGNSLLNDFHHPEMQIPDTMSADIHDWYSWKKFNYTKRGAILPELETEAVDAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-06-18" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GCN1</accession>
    <name>A0AAD5GCN1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptidyl-prolyl cis-trans isomerase</fullName>
            <shortName evidence="5">PPIase</shortName>
            <ecNumber evidence="5">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_017879</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the cyclophilin-type PPIase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="5"/>
    <dbReference type="EMBL" id="JAMZMK010009476">
        <property type="protein sequence ID" value="KAI7735521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016018">
        <property type="term" value="F:cyclosporin A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01926">
        <property type="entry name" value="cyclophilin_ABH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000001">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071:SF561">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="6">
        <location>
            <begin position="88"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7735521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="265" mass="28627" checksum="55AFB5C1E22D42C0" modified="2024-05-29" version="1">MALSFTSLSNIGSISAPRTSPPVVRSCLKTHNKLSKCKVLASSTLLSGSLRLSSSTDTSLRHKSRAFCVRASSAQEVATQAKVTNKVFFDISIGNPVGNSVGRIVIGLFGDEVPQTAENFRALCTGEKGFGYKGSAFHRVIKDFMIQGGDFDKGNGTGGKSIYGRTFKDENFNLTHTGPGIVSMANAGPNTNGSQFFICTVKTPWLDQRHVVFGQVLEGMDVVKLIESQDTXXXXAQQRELLSVIVVSFHWSSQLRPAGVIYELL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GDE3</accession>
    <name>A0AAD5GDE3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_029535</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009034">
        <property type="protein sequence ID" value="KAI7737409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GDE3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000095">
        <property type="entry name" value="protein STRUBBELIG-RECEPTOR FAMILY 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052101">
        <property type="entry name" value="Plant_StressResp_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47983:SF3">
        <property type="entry name" value="OS05G0135800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47983">
        <property type="entry name" value="PTO-INTERACTING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="1"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7737409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="186" mass="20633" checksum="B85E5BB504B8023B" modified="2024-05-29" version="1">MVFINAFGWLASSGIPTQGRKGVKATQGGPSLSWGQRVKIAVEAAKGLEYLHETTQPNIIHRDIKSSNVLLFNNYVAKIADIDISNHYPDMSVRSDSTRAFNFGYHAPEYAMTGQLTTKSDVYTFGVVLLELLTGRKPVDHTLPRGQQSLVTWATPRLIEDKVHTCVDTRLNNEYPLKAVAKVFLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GDX1</accession>
    <name>A0AAD5GDX1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_027385</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008925">
        <property type="protein sequence ID" value="KAI7737919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GDX1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7737919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="92" mass="10371" checksum="AFBDB9A47C9C994D" modified="2024-05-29" version="1">MVEIDIYTIEATSSIVRVEEPFRLNFQLCSHTHVGEFSEFEGRLIKVCLCDPCGDGLYQEYSYSHSKGGVIGRVVKQVSMVKGMLGIFFDQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GEV3</accession>
    <name>A0AAD5GEV3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">G3BP-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_018413</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008571">
        <property type="protein sequence ID" value="KAI7739765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GEV3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.50:FF:000003">
        <property type="entry name" value="Nuclear transport factor 2 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002075">
        <property type="entry name" value="NTF2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018222">
        <property type="entry name" value="Nuclear_transport_factor_2_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039539">
        <property type="entry name" value="Ras_GTPase_bind_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10693:SF29">
        <property type="entry name" value="GB|AAD20086.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10693">
        <property type="entry name" value="RAS GTPASE-ACTIVATING PROTEIN-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02136">
        <property type="entry name" value="NTF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50177">
        <property type="entry name" value="NTF2_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="NTF2" evidence="5">
        <location>
            <begin position="14"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="342"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="137"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="190"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="284"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="418"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="195"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="284"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="324"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="445"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7739765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="458" mass="51103" checksum="B199E5AF417A7A21" modified="2024-05-29" version="1">MANSYSSSVTASQVGSYFVQQYYQVLQQQPEFVHQFYADSSTMVRVDGESTETASAIFQIHSLIQSLHFSGIEIKTINSLESWSQGIVVVVSGSVKSKLFNGWKKFVQTFFLAPQEKGYFVMNDIFHFVSEEVTHQRSPPVASEAKVDPQPVTSELPVGEDALEVEARENLNSLNLEGNNEDDYYNASHETHLQEEEEEEDDEDYESESYDEQPPLEDNVQNTVDYAQQEQPIHYQNTVVDEYVQDPVHHNTVEYVQQPVSAVEEPVAEPVKFTYASILRSKGKSSPSVFAQPPTTKSATPAPEWHLTPEPVAQPPPPVSSASDYNTQSAQESFTNEEGDSKSVYVRNLPTTVTSLEIYEEFKNFGKIKQDGVFLKNRKDVGVCFAFVEFEDFDGVQRAIEASPIQLAGRQIYIEERRANSSSSSIRGGRGRGGGRGRGDASRGRYGGGSYARGNGFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GGB7</accession>
    <name>A0AAD5GGB7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DExH-box ATP-dependent RNA helicase DExH14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_020825</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008472">
        <property type="protein sequence ID" value="KAI7740289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18022">
        <property type="entry name" value="DEXHc_ASCC3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18795">
        <property type="entry name" value="SF2_C_Ski2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000198">
        <property type="entry name" value="Activating signal cointegrator 1 complex subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3380.10:FF:000002">
        <property type="entry name" value="Activating signal cointegrator 1 complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000004">
        <property type="entry name" value="RNA helicase, activating signal cointegrator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000012">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3380.10">
        <property type="entry name" value="Sec63 N-terminal domain-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050474">
        <property type="entry name" value="Hel308_SKI2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004179">
        <property type="entry name" value="Sec63-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057842">
        <property type="entry name" value="WH_MER3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961:SF4">
        <property type="entry name" value="ACTIVATING SIGNAL COINTEGRATOR 1 COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961">
        <property type="entry name" value="DNA POLYMERASE THETA, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G05260)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02889">
        <property type="entry name" value="Sec63"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23445">
        <property type="entry name" value="WHD_SNRNP200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00973">
        <property type="entry name" value="Sec63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158702">
        <property type="entry name" value="Sec63 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="6">
        <location>
            <begin position="181"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="7">
        <location>
            <begin position="384"/>
            <end position="553"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7740289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="914" mass="103991" checksum="7167354950E45920" modified="2024-05-29" version="1">MKEREIGAMIRYAPAGRVVKQYLGFFPSIILSATISPITRTVLKVDLVITPDFVWKDRWHGAAQRWWILVEDSDNDHIYHSELFTLTKRAAKGDPQKLSFTVPIFEPHPPQYYIRAVSDSWLQAEAFYTISFKNLALPESHTTHTELLDLKPLPVTALGNKAYEELYRFSHFNPIQTQAFHVLYHTQNNILLGAPTGSGKTISAELAMLHLFNSAPDMKVIYIAPLKAIVRERMNDWKNGLVTKLGKKMVEMTGDYTPDMMALMSADIIISTPEKWDGISRNWHSRSYVTKVGLMILDEIHLLGADRGPILEVIVSRMRYISSQTERPVRFVGLSTALANAQNLADWLGVGEIGLFNFKPSVRPVPLEGYPGKFYCPRMNSMNKPTYAAICTHSPTKPVLIFVSSRRQTRLTALDLIQFAASDEHPVQFLAMPEDALQMILSQVLVCTSTLAWGVNLPAHLVIIKGTEYYDGKAKRYIDFPITDILQMMGRAGRPQYDQHGKAVILVHEPKKSFYKKFLYEPFPVESSLRDQLHDHINAEIVSGTISHKQDAVHYLTWTYLFRRLMVNPAYYGLEDCDPESLSSYLSSLVQNTFEDLEDAGCIKMDEENVEPLMLGSIASQYYLKYMTISMFASNIGPDTSLEVFLNILSAASEYDELPVRHNEENYNEALSAKVPLAVDKNRLDDPHVKTNLLFQAHFSQVELPISDYYTDLKSVLDQSIRVIQAMIDVCANSGWLSSSITCMHLLQMVMQGMWFERDSPLWMLPCMTEDLVTSLQKGGISNVQQLLNLPNMNMQSRFGNIASRLQQDLQHFPRIQVKLQIQGRDADSDSIPSLSIRLEKSNRRRTSRAFTPRFPKLKDEAWWLVLGNTSTSELHALKRISFTDRLVTRMKLPSTTPSFQGVXXXSFRLLSWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GIF4</accession>
    <name>A0AAD5GIF4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_028849</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the plant acyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008203">
        <property type="protein sequence ID" value="KAI7741431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GIF4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050266">
        <property type="term" value="F:rosmarinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.10:FF:000015">
        <property type="entry name" value="Spermidine hydroxycinnamoyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.10:FF:000008">
        <property type="entry name" value="Tryptamine hydroxycinnamoyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050317">
        <property type="entry name" value="Plant_Fungal_Acyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31642:SF336">
        <property type="entry name" value="HYDROXYCINNAMOYL-COENZYME A: SHIKIMATE_QUINATE HYDROXYCINNAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31642">
        <property type="entry name" value="TRICHOTHECENE 3-O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02458">
        <property type="entry name" value="Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="204"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7741431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="441" mass="48823" checksum="ADF5770604B8D0DD" modified="2024-05-29" version="1">MGSDHKKMNININQSSYVQPSKPTKPCTQQLWTSNLDLVVGRIHILTVYFYRPNGCSNFFDPVVMKKALADVLVTFYPMAGRLSKDQNGRVVIDCNDEGVLFVEAESDSSLDDFGEFTPSPELRRLTPTVDYSGDISSYPLFFAQVTHFKCGGVGLGCGVFHTLADGLSSLHFINTWSDMARGLSIAIPPFIDRTLLRARDPPTPTFDHIEYHPPPSMKLDSQKPESSRKPSTTILKLTLDQVNALKAAAKNEGGNTNYSTYEILAAHLWRCACKARGLPDDQLTKLYVATDGRSRLSPQLPPGYLGNVVFTATPIAKSSDLTAQPLANAASLIRTTLSKMDNDYLRSAIDYLEVQPDLSALIRGPSYFASPNLNINTWTRLPVHDADFGWGRPVFMGPACILYEGTIYVLPSPNNDRSMSLAVCLNADEQPLFEKFLYDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GIL9</accession>
    <name>A0AAD5GIL9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_001749</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010007617">
        <property type="protein sequence ID" value="KAI7743982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GIL9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000596">
        <property type="entry name" value="CK1 family protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="1"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="289"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="360"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="391"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7743982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="437" mass="49434" checksum="BD5E97AC10803AA2" modified="2024-05-29" version="1">MDRVVGGVNLHTGEECAVKLEPTKTKHPQLHYESKIYMLLQGGSESLNLAGVPNLKWFGVEGEYNAMAIDLLGPSLEDLFNYCNRKFSLKTVLMLADQLINRVEYMHARSFLHRDLKPDNFLMGLGRKANQVYIIDFGLAKKYRDLQTHRHIPYRENKNLTGTARYASVNTHLGVEQSRRDDLESLGYVLMYFLRGSLPWQGLKAGTKKQKYDRISEKKMSTSIEALCKSYPSEFSSYFHYCRSLKFEDKPDYSYLKRLFRDLFIREGYQFDYVFDWTMLKYPQISASSRGRVQGGNAGQNVGTSAERPGRTSAVQDVREALSRRNPIGGPRLELSRNRTPDVVASSKDVQPDSEKVRTSRNGSSSKRAAFLTNNKPSSSADATDGRTSRHMSSSGARLSTIQRGYDNKPSPAGLSGTSKGGREDLLRSFDLLQIRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GJE0</accession>
    <name>A0AAD5GJE0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DM2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_024307</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008104">
        <property type="protein sequence ID" value="KAI7741928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GJE0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd10568">
        <property type="entry name" value="SWIB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.245.10">
        <property type="entry name" value="SWIB/MDM2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019835">
        <property type="entry name" value="SWIB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036885">
        <property type="entry name" value="SWIB_MDM2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003121">
        <property type="entry name" value="SWIB_MDM2_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13844">
        <property type="entry name" value="SWI/SNF-RELATED MATRIX-ASSOCIATED ACTIN-DEPENDENT REGULATOR OF CHROMATIN SUBFAMILY D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02201">
        <property type="entry name" value="SWIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00151">
        <property type="entry name" value="SWIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47592">
        <property type="entry name" value="SWIB/MDM2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51925">
        <property type="entry name" value="SWIB_MDM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DM2" evidence="1">
        <location>
            <begin position="183"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7741928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="402" mass="45787" checksum="7F3A32600F162CFE" modified="2024-05-29" version="1">MLELTPAARLKKKPKLNGKRLQERVASILPPSALYTQLREFESLIDAALVKKKVDVQEAVKFPPYVQKTLRIYIFNTFANQSRTNSGKPNAEPPTWTLKIVGRILEEGMDPDQAGLMSKPNLMYPKFSSFVKRVAISLDQKLYPDNHIIVWDSSRSTAHHEGFEVKRKGDEEFIVKIRFEMNYVPEKFMLSSPLMEVLGTEVDTRARIVAALWQYVKYKKLQNVDDPSCFNCDPQLLKVFGEEKVRFSMVSQTMSNHLSPLPPILLEHKIKLSGDSPAGNACYDVLVDVPLLVQKDMKAVLANKARKKEIEACDEAILTTIRKINEHRRRRAFFLGFSESPVKFIDALIESQGRDLKLLAGDANRNAEKESTRADFYSEPWVEDAVIQYLNTRPARIADEGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GKD1</accession>
    <name>A0AAD5GKD1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_031830</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007031">
        <property type="protein sequence ID" value="KAI7746140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GKD1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051283">
        <property type="entry name" value="Sec_Metabolite_Acyltrans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31896">
        <property type="entry name" value="FAMILY REGULATORY PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G14730)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31896:SF12">
        <property type="entry name" value="HXXXD-TYPE ACYL-TRANSFERASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02458">
        <property type="entry name" value="Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7746140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="156" mass="17393" checksum="3E7D11FEC262A8E1" modified="2024-05-29" version="1">MAADNRKRLSPPLPETYLGNSLHSVRAIATAGQLLHHNIGWAAWRLHQAVADHDDRVIREFVDSWLRNPFVYTVSRFFDPNCIQIGSSPRFDMYGNKFGLGEGLAVLSGYANKFDGKVTVYPGRDGGGSMDLEICLLPENMQALESDEEFMSVITC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GL58</accession>
    <name>A0AAD5GL58_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF4228 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_011752</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006861">
        <property type="protein sequence ID" value="KAI7746997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GL58"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025322">
        <property type="entry name" value="PADRE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33413:SF30">
        <property type="entry name" value="D-RIBOSE-BINDING PERIPLASMIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33413">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14009">
        <property type="entry name" value="PADRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="97"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="135"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7746997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="161" mass="17865" checksum="EAF6E03959431BBC" modified="2024-05-29" version="1">MGNCQAVDAAALVIQHPSGKIERMYWSVTAGEIMKMNPGHYVSLIIPLPGEEKQDGSGNKTVRFTRVKLLRPTDTLVLGRAYRLVTSHEVMKVLRAKRQAKMNKEQPKTTVEKENDGDELESSSRVLAHDRTIKQRSGSFSKSKTWRPSLQSISEAATASG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GLS6</accession>
    <name>A0AAD5GLS6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Leucine-rich repeat-containing N-terminal plant-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_018103</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007553">
        <property type="protein sequence ID" value="KAI7744268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GLS6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45631:SF45">
        <property type="entry name" value="LEUCINE-RICH REPEAT (LRR) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45631">
        <property type="entry name" value="OS07G0107800 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="3">
        <location>
            <begin position="55"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7744268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="103" mass="11232" checksum="4D3859310D9B8B86" modified="2024-05-29" version="1" fragment="single">DLNVTTKGVTVYGTEWPLSGDTQITLTPRSDMPVGPTINAGEIYQILPLAVTTLARDVLALQMLKQSLKNPPDDWNGDPCLPKEHPWFGIGCTEGKQVRVTSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GLV8</accession>
    <name>A0AAD5GLV8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Trichome birefringence-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_017024</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PC-esterase family. TBL subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006684">
        <property type="protein sequence ID" value="KAI7747807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GLV8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016413">
        <property type="term" value="F:O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029962">
        <property type="entry name" value="TBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026057">
        <property type="entry name" value="TBL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025846">
        <property type="entry name" value="TBL_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32285:SF357">
        <property type="entry name" value="PMR5 DOMAIN, PC-ESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32285">
        <property type="entry name" value="PROTEIN TRICHOME BIREFRINGENCE-LIKE 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13839">
        <property type="entry name" value="PC-Esterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14416">
        <property type="entry name" value="PMR5N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Trichome birefringence-like N-terminal" evidence="9">
        <location>
            <begin position="76"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="Trichome birefringence-like C-terminal" evidence="8">
        <location>
            <begin position="130"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="432"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="433"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="444"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF14416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7747807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="454" mass="51554" checksum="0630552E162B9078" modified="2024-05-29" version="1">MTHMDFLKKFKKFKPFELGCICISLCFILLLFFLDYRSVGSGFIRARGLGGVWFGSSKFRHSGHERFGFLEMGRVEKCDIFDGSWVWDESYPLYEGKDCLLLDDGFRCSENGRVDRFYTKWRWQPKDCNLPRFDAKTMLERLRNRRLAFVGDSIGRNQWESLLCMLSTAVANKSSIYEVNGRSITKHSGFLVFKFADFNCTIEYYRAPFLVLQRRPPPGSPKQVRTTLKLDKMDWGAGKWKGADIIVLNSGHWWNSEKTTRVGCYFQEADGVKMNMSIETAYRKSIETIVDWISKEVDTNKTQVIFRGYSPVHFRGGDWKSGGSCHLETLPDLSSSPVSPATMFYSNIIGSVLSKHGNRSSAQTKLELLNVTYMSSKRKDGHASVYYSGPKNGGPAPLHRQDCSHWCLPGVPDSWNELLYAVFLKREYSRAGSSPATTAASPPENERSLATDGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GM41</accession>
    <name>A0AAD5GM41_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_017580</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006625">
        <property type="protein sequence ID" value="KAI7747955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GM41"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7747955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="67" mass="7837" checksum="6B1C1D21C474614E" modified="2024-05-29" version="1">MFLNLTRSITLAIISIIRFVICRPTMFLFHALFISTTIMIFSLPKRLSLRLQILGNGRLVWGRQRMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GRA9</accession>
    <name>A0AAD5GRA9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_020814</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006401">
        <property type="protein sequence ID" value="KAI7749128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GRA9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7749128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="131" mass="15513" checksum="0D0A47BF0563332B" modified="2024-05-29" version="1" fragment="single">MTFKEKKQETEKKWRLAKWLRLNWVKLKRKQGVWLRLVRFDYDFKRNRYICGVIIGVAIGSSTPPFPLPEFLLGVARLLPPYQHLSTPYDLFLIDKLERRVVIGKQVAGATKWKVEKTKVKVVLPFQFHTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GRN6</accession>
    <name>A0AAD5GRN6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">(+)-lariciresinol reductase</fullName>
            <ecNumber evidence="5">1.23.1.1</ecNumber>
            <ecNumber evidence="6">1.23.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">(+)-pinoresinol reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_015881</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Dimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NmrA-type oxidoreductase family. Isoflavone reductase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.23.1.1" evidence="5"/>
    <dbReference type="EC" id="1.23.1.2" evidence="6"/>
    <dbReference type="EMBL" id="JAMZMK010005665">
        <property type="protein sequence ID" value="KAI7752530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GRN6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010284">
        <property type="term" value="F:lariciresinol reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010283">
        <property type="term" value="F:pinoresinol reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902125">
        <property type="term" value="P:(+)-pinoresinol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902138">
        <property type="term" value="P:(-)-secoisolariciresinol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05259">
        <property type="entry name" value="PCBER_SDR_a"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008030">
        <property type="entry name" value="NmrA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050608">
        <property type="entry name" value="NmrA-type/Isoflavone_red_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045312">
        <property type="entry name" value="PCBER-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43349:SF47">
        <property type="entry name" value="NMRA-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43349">
        <property type="entry name" value="PINORESINOL REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05368">
        <property type="entry name" value="NmrA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0521">NADP</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NmrA-like" evidence="9">
        <location>
            <begin position="4"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="NmrA-like" evidence="9">
        <location>
            <begin position="311"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="domain" description="NmrA-like" evidence="9">
        <location>
            <begin position="611"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="domain" description="NmrA-like" evidence="9">
        <location>
            <begin position="727"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="840"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00042771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00043173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF05368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7752530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="840" mass="94045" checksum="73B515F666AC7743" modified="2024-05-29" version="1" fragment="single">MGLKSKVLIIGGTGYLGKRLVKASLQQGHETYVLQRPEIGVDIEKIEMLLSFKKQGARLVIGSFEDHRSLVEALKQVDVVICAVSGVHIRSHQILLQLKLVDAIKEAGNIKRFLPSEFGTDPARMADAMEPGRVTFDDKMVVRKAIEEAGIPFTYVSANCFAGYMVGGLCQPGHILPSRDSVTLFGDGNKKSIFVDEDDIAAYTIKTIDDPRTLNKTLYIRPPANILSQREVVGLWEKLIGKQLHKSSLSEQQFLNIMKEQDYAEQVGLTHYYHVFYDGCLANFEIGKDAEEASILYPDIKYIKHKDMGIKSRVLITGGTGHLGKRLVKASLEQGHETYVLQRPEIGVDIEKIQMLLSFKKQGARLVIGSFDDHCSLVEALKQVDVVICAISGMHIRSHQILLQLKLVDAIKEAGNIKRFLPSEFGMDPARMADAIEPGRVTFDDKMVVRKAIEEAGIPFTYVSANCFAGYMVGGLCQPGHILPSRESVTLFGDGNVKAIFVDEDDIAAYTIRTIDDPRTLNKTLYLRPPANILTQREVVGLWEKLIRKELHKSCLPEQEFLNIMKEQGYAEQVGLTHYYHVYYDGCLANFEIGKDSEEASVLYPDVKYIKSRVLIIGATGYLGKRLVKASLEQGHETFVLQRPEIGVDIEKIQILLSFKKQGARLRFLPSEFGTDPARMSDAMEPGRVTFDDKMVVRKAIEDAGIPFTYVSANCYAGYFIGGLCQPAIFVDEDDIAAYTIKTIDDPRTLNKTLYIRPPANTLSQREVVGLWEKLIGKQLHKSSLSAQQFLNILKEQGYGEQVGLTHYYHIFYDGCLTNFEIGKDAEEASVLYPDIKYIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GT67</accession>
    <name>A0AAD5GT67_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_018080</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005699">
        <property type="protein sequence ID" value="KAI7752394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GT67"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7752394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="77" mass="8734" checksum="EBEA4426BACD5A1E" modified="2024-05-29" version="1" fragment="single">CGGDLNLLTVQKNPPPRSRRPPFSSTIVTVQPTPHTKFPSPTFIIALLPFRYVTGLMVLSGWVVDEDREDHEMFLLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GU46</accession>
    <name>A0AAD5GU46_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_002630</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="JAMZMK010005794">
        <property type="protein sequence ID" value="KAI7751903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GU46"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17917">
        <property type="entry name" value="DEXHc_RHA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF221">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DHX34-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="12">
        <location>
            <begin position="28"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="13">
        <location>
            <begin position="248"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="11">
        <location>
            <begin position="732"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="10">
        <location>
            <begin position="732"/>
            <end position="759"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7751903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="1057" mass="119400" checksum="A6AA31876B481310" modified="2024-05-29" version="1">MAEPLTSSALSSESFKNLPIMSLRSKIVEKIMENRVTLIVGETGCGKSSQVPQFLLEENIEPIICTQPRRFAVVAVAKMVAKSRNCVIGGEVGYHIGHSRVMSSSSKIIFKTAGVLLEEMREKGMNALKYKVIILDEVHERSVESDLVLACVKQFLIKNTGLRVVLMSATADITRYKEYFGDIDRGERVEVLGIPSSSQHTLYQRRVSYLEQVVELLEMSSESLSLKYCSGPSPDLSEATMNEEVHQLIHNLVLHIHKNEPDMEKSILIFLPTYHALEQQWHAMSRYRSAFKVHILHSSIDTEQALNAMRILKSHRKVILATNIAESSVTIPKVAYVIDSCRSLQVYWDANRKTESSEIVWVSESQAEQRRGRTGRTCDGEVYRLVTEPFFGQLEKYEAPSILKLSLRQQVLLTTCAESRAINDPKGKAKLLFPYTILLYKVMDPPKPEVVEDALELLVHIHALEKTPPRGRYEPTFYGRLLSSFSLSFDASMVILKFGEAGMLREGIIIGILMDTQPLPIIRPFGQETLFSEFTDGYYSQHGKLTGLMGRKEVIFMANFCAFQFWQRTFQANLRLERLKHLLKFDATTDTQITSLKIEEEWCFFHNLVQSSLQHIASIYDSVLNNMHRYRPNFLATSNGLPSYYEPYEFQHTCLLTVDRNDDSEPPATDDDLSNPLFETKKCVALPFVGKNGFNKNEVAFRLAESIKEIRVCYHGDSTGNQQTQDLVSYNGQEASLCRYFASGTCNKGSECLFSHSLEAKKPVCKFFLSLQLETKAYYHSGKMGGQGWVLDNISKISPDQLSLPNSEHTSCAAEDISADASLLLSFFPSPDDGCLLLFDDFDIQFSSNFSHFYNPSSIVCTTSAVKSSNDPFLQDLRIVWGLSHPHGTIISKAANNSIPWEEVKCAVWIPKFGNDVENWEEQKGLIKTFFEYLAIRILGDALYEVQVILVLNNLRFAQLQAGGVGLTFGFEGMSLLVFFGVFGLNVESLGRENFFYLRESFAFDEYSFGKLYDGVATKKPMLASKAIVYVFDLHPPTDIQFGDYTSLLHNQLHNIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GVQ2</accession>
    <name>A0AAD5GVQ2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_029143</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the glycosyl hydrolase 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010004627">
        <property type="protein sequence ID" value="KAI7754031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GVQ2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008422">
        <property type="term" value="F:beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001360">
        <property type="entry name" value="Glyco_hydro_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353:SF175">
        <property type="entry name" value="BETA-GLUCOSIDASE 18-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353">
        <property type="entry name" value="GLYCOSYL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00232">
        <property type="entry name" value="Glyco_hydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00131">
        <property type="entry name" value="GLHYDRLASE1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7754031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="172" mass="20045" checksum="A79AA6F2771A9427" modified="2024-05-29" version="1">MAYERWAFPQKQCSQPFGNCLAGNSDATQGRLVGLVAHCFMYEPLTNSYFDQKAAERTMLFNVGYCSPNVLDAVVEELLNDFKRIEFHQTYLALAEAIKNGADVRGYFIWSLMDNYEWNYGYKVRFGLHYVDRKTYGRIPKLSARWYQEFLKNNSLVDVPSIRSRKSLLISY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-04-02" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GWR7</accession>
    <name>A0AAD5GWR7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_013726</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000005">
        <property type="protein sequence ID" value="KAI7758365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043067">
        <property type="term" value="P:regulation of programmed cell death"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16649">
        <property type="entry name" value="mRING-HC-C3HC5_CGRF1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42647:SF12">
        <property type="entry name" value="BOI-RELATED E3 UBIQUITIN-PROTEIN LIGASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42647">
        <property type="entry name" value="SBP (S-RIBONUCLEASE BINDING PROTEIN) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="274"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7758365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="336" mass="38263" checksum="5103E767A459725D" modified="2024-05-29" version="1">MFLSVNEAPHLSLYPPPPPHLLLFNPMMMMLDAIDTQQSTLTNRVPFHPMHPLSADTFLPMYSSSLANYYPDPDSDLTYTTTLPVSRKRSRDCSYMGSMDHSASFTFLGEDISYQMRQQQAEIDQFVARHAEKLRTDIEERRKRNSRKLITALEEGVTHIFRAKEEEIMKMMKLNYALEEKLKSLCIENQIWREIAQTNEATANALRTNLKQVLDQLVNDNCSGYGKHRTKEGENDVALANDAESCCESNNKDEERTLAEQDSGESNVKNKRLCKSCGKGESCVLLLPCRHLCLCTVCASSVHVCPICKSTNNISVHVXXXFDWNIESYRKLSTMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GWT1</accession>
    <name>A0AAD5GWT1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mitogen-activated protein kinase</fullName>
            <ecNumber evidence="9">2.7.11.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_013098</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="9">Activated by threonine and tyrosine phosphorylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.24" evidence="9"/>
    <dbReference type="EMBL" id="JAMZMK010000003">
        <property type="protein sequence ID" value="KAI7758410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004707">
        <property type="term" value="F:MAP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010225">
        <property type="term" value="P:response to UV-C"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07858">
        <property type="entry name" value="STKc_TEY_MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000013">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000046">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003527">
        <property type="entry name" value="MAP_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01351">
        <property type="entry name" value="MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="36"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7758410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="369" mass="42438" checksum="37D1018FDE41731B" modified="2024-05-29" version="1">MESSSGEHIKGTPTHGGRYVQYNVYGNLFEVSRKYVPPIRPVGRGVNGIVCAAMNAETREEVAIKKIGNAFDNRVDAKRTLREIKLLRHMDHENVIAIKDIIRPPLKENFNDVYIVYELMDTDLHQIIRSNQPLADDHCRYFLYQMLRGLKYVHSANVLHRDLKPSNLLLNANCDLKIGDFGLARTTSETDFMTXXXXTRWYRAPELLLNCSEYTAAIDIWSVGCILGEIMTRQPLFPGKDYVHQLRLITELIGSPDDASLGFLRSDNARRYVRQLPQYPKQQFSARFPNKSPGALDLLEKMLVFDPNRRITADEALCHPYLAPLHDMNEEPVCPRPFSFDFEQPTCTEENIKELIWKESVKFNPDPTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BJZ2</accession>
    <name>A0AAD5BJZ2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_015441</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012184">
        <property type="protein sequence ID" value="KAI7724580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BJZ2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46992">
        <property type="entry name" value="GYF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46992:SF1">
        <property type="entry name" value="GYF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="254"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="425"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="12"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="254"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="268"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7724580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="550" mass="61430" checksum="39A12EBA4024AF70" modified="2024-05-29" version="1">MPGLTGQDLEHIWALQLQQQQQQQQQQQLQQQQRQIELQQQMLLKEQQSRQQMLLEQLVHNQRSHTDPTRSHNVHDQMLLKHQILSEMQQREHLIQAKYGHQGHTNDLLEMMMRAKHGQVSSLENQLLQREQLRQLGLRQRVEMEEERQLGSDWFHLANSPGLGIGSSDLSHIEQNLLMQEQIQRGIYDPKLSPYERSLSMSGGGSGMNPDHVGGFQHPLGSNSFHRSHADVMEGQMSNEWMESRIQQLHINNEKQKRDMESRRSSEDQSQWMSGGTSDDTSKRLLMELLHQKPSNQPAEPLDINNGLNHPFGVGSYGLNPGARIDDTVLGFKGNAESQNPINIINGLGAETQESMVTQQGGVTAVDHRHNSLGGGGLYVDNIGVGPPDFFALDAEDRATTTSKRPENILLKRPPVARTALSHESLAELASKSDARGRSVPTIISPEGGRREAGGNAANQVTENTMDVRFRRTSSWSDTDVSETASFSDMLKSNAKKADNHAAALENADGQGGKVGKKKGKKGRQIDPALLGFKVTSNRIMMGEIQRIED</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BMS7</accession>
    <name>A0AAD5BMS7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Tubulin alpha chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">M8C21_019157</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">AA19_3_7</strain>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12 14">Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="14">Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the tubulin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012076">
        <property type="protein sequence ID" value="KAI7725059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BMS7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02186">
        <property type="entry name" value="alpha_tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.600:FF:000005">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.20:FF:000001">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000007">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.600">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002452">
        <property type="entry name" value="Alpha_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023123">
        <property type="entry name" value="Tubulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01162">
        <property type="entry name" value="ALPHATUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10 14" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 14" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="16">
        <location>
            <begin position="49"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="17">
        <location>
            <begin position="214"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="396"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="397"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAI7725059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="416" mass="45640" checksum="52EA5DA7D39CF883" modified="2024-05-29" version="1">MRECISVHIGQAGIQVGNACWELYCLEHGIQPDGQMPSDKTVGGGDDAFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRCGTYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLDRIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTSVVEPYNSVLSTHSLLEHTDVISSLTASLRFDGALNVDVTEFQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITNTAFEPSSMMAKCDPRHGKYMACCLMYRGDVVPKDVNAAVATIKTKRTIQFVDWCPTGFKCGINYQPPTVVPGGDLAKVQRAVCMISNSTSVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGAEGGDDEEDEGDEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BN32</accession>
    <name>A0AAD5BN32_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_020243</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the peptidase A1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011941">
        <property type="protein sequence ID" value="KAI7725421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BN32"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06098">
        <property type="entry name" value="phytepsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000044">
        <property type="entry name" value="Lysosomal aspartic protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000115">
        <property type="entry name" value="Lysosomal aspartic protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.225.10">
        <property type="entry name" value="Saposin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033869">
        <property type="entry name" value="Phytepsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007856">
        <property type="entry name" value="SapB_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011001">
        <property type="entry name" value="Saposin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF80">
        <property type="entry name" value="ASPARTIC PROTEINASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05184">
        <property type="entry name" value="SapB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47862">
        <property type="entry name" value="Saposin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="6 8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 9" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041937551" description="Peptidase A1 domain-containing protein" evidence="10">
        <location>
            <begin position="22"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="11">
        <location>
            <begin position="90"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="121"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7725421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="508" mass="54900" checksum="4E11A0816DECFDAF" modified="2024-05-29" version="1" fragment="single">VNMERTLTIFLLLSTLLCARAFSTSGDGLIRVNLKKLKFDDTNRVTSDLVLDEGDLLIESIRKYKQSANNLVEQQEPDIVSLKNYMDAQYYGEIGIGTPQQKFTVVFDTGSANLWVPSSKCIFSLACLVHTRYRSGQSQTYKANGTPAAIQYGTGSISGYFSQDNVQLGDLAVIDQMFIEATREPGVTFLAGKFDGILGLGFKNISVGNAVPVWENMVNQHLVKEPVFSFWLNRKSENGEGGEIVFGGIDPKHFTGEHTFVPVTQKGYWQFDMGDVLIGGKPSGFCTNGCSAIADSGTSLIAGPSEVVKQINDAIGAAGVLNEEYQRAVKLLGSHVFDQLVNSVGNATQVCAHIGACPRQPGASSIGIKSVVDSSDDVSSGFEDTPSCTACKMIVSWAHSELMENKTRDSIINLGSDNPLDQSIIDCAKISSMPTISFTIGGKEFQLSPDQYILKVGEGASARCISGFIALDIPPPRGPLWILGDTFMRPYHTVFDYGNARVGFAQAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BPL4</accession>
    <name>A0AAD5BPL4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein unc-13 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_026937</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011404">
        <property type="protein sequence ID" value="KAI7727276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BPL4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014770">
        <property type="entry name" value="Munc13_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014772">
        <property type="entry name" value="Munc13_dom-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057984">
        <property type="entry name" value="PATROL1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008528">
        <property type="entry name" value="unc-13_homologue"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31280:SF1">
        <property type="entry name" value="OS03G0138600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31280">
        <property type="entry name" value="PROTEIN UNC-13 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25761">
        <property type="entry name" value="TPR_PATROL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51258">
        <property type="entry name" value="MHD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51259">
        <property type="entry name" value="MHD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MHD1" evidence="2">
        <location>
            <begin position="497"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="domain" description="MHD2" evidence="3">
        <location>
            <begin position="780"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="75"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7727276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="947" mass="106054" checksum="FAA507EF633DBE06" modified="2024-05-29" version="1">MLSDLSCPFETLDGLDSDDIQATAYEIFFTSCRSSPGFGGRNAIQFYSSDGDNDSKTSRFKRAVGLKMLKRSVSSRRNNSNNNNNNCVGISGRRPRRPLTSAEIMREQMKVSEASDNRLRKTLMRTLVGQMGRRAETIILPLELIRHLKPSDFDDSNEYHMWQKRQLKVLEAGLIVHPINPLDRTNNLAMCLKDIIYSSHEKPIDIGKASETMRTLCNCAVSLAWRSADGSPADVCHWADGFPFNVNLYASLLCSVFDLKEQTCVLDEVDELLELMKKTWTTLGFNKPMHDLCFAWVVFQQYVMTGQVESDLLSASLTMLTEVANESKKVDKDHVYVKMLSSVLTSMTMWCEKGLMDYHGCFTVEAMEHILPLVFSATRILEEDVPGYAIDRGDASAGNKVDQYTRSSLRNAFSKMVENGIHVISRKMSFQQVCETLVRLAQETEELAVKEKKSFSMVLKKWHPISAGVAAATLHSCYGALLKEFLTCNSDITNEMLTVLQRADKLEKVLVNIVVEDSVECEDGGKAVVREMVLYDVDSIIVKFLGQSVSGRLKRIKVVLHRAKETETWNPKSKTEPYSQSAVELMKQAREVVVSFFDIPIAIAENLVREFTDGIEEILRDYAAFVGSCGSKQNYIPTLPPLTRCSRDSRLIKLWKKATPCTVASLAPYHLGLGLGLGVGLEEDGNNTRPSTSRGTQRLYIRLNTLHYIISQLTLLDKCFSHSFKNLPSHKNQRHAHFEYTRSAILGIAQHVSEVAAYRLIFLDSNSVMYGSLYEGNVVNARISPALRVLKQNLTLLCAIVTERAQALAVKEVMRASFEAFLMVLIAGGSARCFTRADHEMIEEDMKHLKRVFGEGVMAGDVVEKEAETVEGVVNLMGKTTEQLVDDFTKLTCETSGVAMGQTLPMPPTTGKWSSSDPNTILRVLCHRKDRVANSFLKTTFHLAKRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BS11</accession>
    <name>A0AAD5BS11_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Serine aminopeptidase S33 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_009509</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011386">
        <property type="protein sequence ID" value="KAI7727403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BS11"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000192">
        <property type="entry name" value="Caffeoylshikimate esterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051044">
        <property type="entry name" value="MAG_DAG_Lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11614">
        <property type="entry name" value="PHOSPHOLIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine aminopeptidase S33" evidence="1">
        <location>
            <begin position="39"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7727403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="313" mass="35056" checksum="3456D6C4B1574E14" modified="2024-05-29" version="1" fragment="single">TPEHEYYTSQNLRNTKSYFQTPNATIFTQSWLPLDPNQPIKATVFMTHGYRSDCSWSFQEICIAYAKWGYAVFAADLIGHGRSDGLHGYIGDMDKAAATSLSYYVSVRMSEEYRNLPAFLFGQSMGGMITMLMYFQSDPDTWSGLIFAAPLLVLPEAATPSKLHLTMYGLLFGLADTWAVMPDPRMGAKAVRDVEKQKILAMNPKLYLGKPRVGTMREVVRVTNYLQQNFERVKVPFFTAHGTADGLASHSGSEMLYEKAATAKEDKCLKLYEGMYHSLINGEPDEDADVVLGDMKAWIDEKAQKFGPKITGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BSU5</accession>
    <name>A0AAD5BSU5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_001979</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011124">
        <property type="protein sequence ID" value="KAI7728885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BSU5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7728885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="106" mass="11408" checksum="8A81A07E119A3156" modified="2024-05-29" version="1" fragment="single">QVESSFQKWPTCLVDPLVAKSGPIGNIFGNKVNTPGSSSMVELQIIWSAASAASLPSRITKSKIIWFVAESPTHAHKKLLGSMSGMEVLNAHGHCQNCSRARTKDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BT50</accession>
    <name>A0AAD5BT50_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_021495</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011092">
        <property type="protein sequence ID" value="KAI7729000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BT50"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000016">
        <property type="entry name" value="Somatic embryogenesis receptor-like kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052059">
        <property type="entry name" value="CR_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47973">
        <property type="entry name" value="CYSTEINE-RICH RECEPTOR-LIKE PROTEIN KINASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="1"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7729000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="235" mass="26583" checksum="1EC6A0C48CCB5445" modified="2024-05-29" version="1" fragment="single">FITEIIFPELNSLILLWVLPDQIQGKPVLDWSRRKNIALGTARGLLYLHEQSDPKIIHRDVKAANILLDEDFEAVVGDFGLAKLLDHRDSHVTTAVRGTVGHIAPEYLSTGQSSEKTDVFGFGILLLELITGQKALDFGKTAANQKGVMLDWVKKLHQDGKLNLMVDKSLKNNFDRVELEEMVQVALLCTQFSPSNRPKMSEVLRMLEGEGLVERWEASQNIETQRFRTQESYPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BTG9</accession>
    <name>A0AAD5BTG9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_018537</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011021">
        <property type="protein sequence ID" value="KAI7729317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BTG9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7729317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="77" mass="8366" checksum="C9AF13606E6EED90" modified="2024-05-29" version="1" fragment="single">SVAARVAKEKKLVSNLIRKLVILFDLKSDKFSSQTSRPSASIAYFGAAKLVNGRQGQLEMGKKNAVLPANEVDFNTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BUJ3</accession>
    <name>A0AAD5BUJ3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glucan endo-1,3-beta-D-glucosidase</fullName>
            <ecNumber evidence="3">3.2.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_020932</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of (1-&gt;3)-beta-D-glucosidic linkages in (1-&gt;3)-beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.39"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the glycosyl hydrolase 17 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.39" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010010930">
        <property type="protein sequence ID" value="KAI7729727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BUJ3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042973">
        <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000005">
        <property type="entry name" value="Glucan endo-1,3-beta-glucosidase 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000490">
        <property type="entry name" value="Glyco_hydro_17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044965">
        <property type="entry name" value="Glyco_hydro_17_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32227">
        <property type="entry name" value="GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00332">
        <property type="entry name" value="Glyco_hydro_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042297584" description="glucan endo-1,3-beta-D-glucosidase" evidence="9">
        <location>
            <begin position="28"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="354"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="373"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7729727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="430" mass="45505" checksum="5BC8723A954A4998" modified="2024-05-29" version="1">MASPPSTTTTTVATFLLLLHAAVVARSQSYIGVNYGRVADNLPPPSATAKLLQTTSFEKVRLYEADSSIIKALANTNIGITIGAANDAIPPMAADVSFAKTWIGANVLPYYPSSKIILINVGNEVLQYGTPELKTQLLPAMKNMQSALEEASLGGKIKVSTVHSMSVLGQSEPPSAGRFDPSVVDLLKGLLEFNKETGSPFMINPYPFFAYQTDPRPETLAFCLFQPNAGRPDSGTNVKYMNMFDAQVDAVKAALNAMSFNDVEIVVAETGWPYKGGDDEAGATIENAKAYNGNLVAHLRSMVGTPLMPGKSIDTYLFALYDEDLKPGKPSERAFGIYNVDLTPIYDANLSKGSQGGLANAPAPTISPPTDAPTTSGATTLPTNTSTTPNTTTPRDSPTARVNASKNDATLDKMGASLIIVVSLAVALML</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BUN7</accession>
    <name>A0AAD5BUN7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein BZR1 homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Protein BRASSINAZOLE-RESISTANT 1 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_028680</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Functions in brassinosteroid signaling. May function as transcriptional repressor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the BZR/LAT61 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011152">
        <property type="protein sequence ID" value="KAI7728751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BUN7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009742">
        <property type="term" value="P:brassinosteroid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008540">
        <property type="entry name" value="BES1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033264">
        <property type="entry name" value="BZR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31506">
        <property type="entry name" value="BES1/BZR1 HOMOLOG PROTEIN 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31506:SF15">
        <property type="entry name" value="BES1_BZR1 HOMOLOG PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05687">
        <property type="entry name" value="BES1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1070">Brassinosteroid signaling pathway</keyword>
    <keyword evidence="3 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BES1/BZR1 plant transcription factor N-terminal" evidence="7">
        <location>
            <begin position="7"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="96"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="195"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="99"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU369040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7728751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="305" mass="32862" checksum="906DDF732B7B8261" modified="2024-05-29" version="1">MTGGTASGRLPSWKERENNKRRERRRRAIAAKIYAGLRSQGNYRLPKHCDNNEVLKALCAEAGWVVEEDGTTYPKGCKPPPSEIAGMSTNISTCSSIQPSPMSSAFPSPAPSYHASPTSSSFPSPTRCENPPNHSSYILPYLCNLSSLPPLRISNSAPVTPPLSSPTARGTKRKPNWEMLSTSALQSFRHPLFAASAPSSPTRRHHIPPATIPECDESDASTVDSGRWVSFQAVPASAAPTSPTFNLVKPASQQPFFQNGLVVQNGPGSEFEFESSTLKAWEGERIHEVGADDLELTLGSGKATC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BXX7</accession>
    <name>A0AAD5BXX7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">glucomannan 4-beta-mannosyltransferase</fullName>
            <ecNumber evidence="11">2.4.1.32</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Glucomannan synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">M8C21_006054</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">AA19_3_7</strain>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>GDP-mannose + (glucomannan)n = GDP + (glucomannan)n+1.</text>
            <dbReference type="EC" id="2.4.1.32"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the glycosyltransferase 2 family. Plant cellulose synthase-like A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.32" evidence="11"/>
    <dbReference type="EMBL" id="JAMZMK010010771">
        <property type="protein sequence ID" value="KAI7730493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BXX7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047259">
        <property type="term" value="F:glucomannan 4-beta-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051753">
        <property type="term" value="F:mannan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000015">
        <property type="entry name" value="Glucomannan 4-beta-mannosyltransferase 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32044">
        <property type="entry name" value="GLUCOMANNAN 4-BETA-MANNOSYLTRANSFERASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32044:SF77">
        <property type="entry name" value="GLUCOMANNAN 4-BETA-MANNOSYLTRANSFERASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13632">
        <property type="entry name" value="Glyco_trans_2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="43"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="360"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="392"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="498"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="14">
        <location>
            <begin position="182"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00051800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00060879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00066505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00076024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF13632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAI7730493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="524" mass="60433" checksum="9059DB7ABE3C5E1C" modified="2024-05-29" version="1">MEKLLTVAYLSELFVGAQDDIAGQIVVIWGQIKVSFVVPFLRIFMYINLVMSIMLFTEMVYMAIVVAFNKLFGKKPQQRYKWEPFQDDVELGSSVYPLVLVQVYKLSIGAACGLSWPSDRIVIQVLDDSTDPLIKGMVEMECESWASKGVNIHYQVRDNRKGYKAGALKEGLKHDYAKQCEYVVIFDADFQPEPDFLTQTIPYLHHNSELGLVQGRWKFVNSNDCLMTRLQEMSLNYHFKVEQEYGSIAFAFFGFNGTAGVWRMAALDEAGGWKDRTTVEDMDLAVRASLKGWKFLYLGSLMVKNELPSVFKAYRYQQHRWSCGPANLFRKMFFEIMRNERVTWWKKLYVLYSFFFVRKIVSHIVTFTLYCVVFPATVLVPEVMIPRWFTVYIGTMITLLSAIGTPRSFYLVVFWVLFENVMSLHRAKATFVGLFETQRVNEWVVTEKHGDASKVKATTQQSLSWFRIGERVHMLELCVGIVLFGCACYDLAYGKYHYYVYLYLQAIAFITMGCGCIGTQVSNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BYI6</accession>
    <name>A0AAD5BYI6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Histone deacetylase interacting domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_028780</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010372">
        <property type="protein sequence ID" value="KAI7731827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BYI6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000118">
        <property type="term" value="C:histone deacetylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1160.11:FF:000001">
        <property type="entry name" value="Paired amphipathic helix protein Sin3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1160.11:FF:000003">
        <property type="entry name" value="Paired amphipathic helix SIN3-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1160.11">
        <property type="entry name" value="Paired amphipathic helix"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013194">
        <property type="entry name" value="HDAC_interact_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003822">
        <property type="entry name" value="PAH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036600">
        <property type="entry name" value="PAH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039774">
        <property type="entry name" value="Sin3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031693">
        <property type="entry name" value="Sin3_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12346:SF0">
        <property type="entry name" value="SIN3A, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12346">
        <property type="entry name" value="SIN3B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02671">
        <property type="entry name" value="PAH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08295">
        <property type="entry name" value="Sin3_corepress"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16879">
        <property type="entry name" value="Sin3a_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00761">
        <property type="entry name" value="HDAC_interact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47762">
        <property type="entry name" value="PAH2 domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51477">
        <property type="entry name" value="PAH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <feature type="domain" description="Histone deacetylase interacting" evidence="7">
        <location>
            <begin position="451"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="246"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="734"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="246"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="269"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="290"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="737"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="785"/>
            <end position="805"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7731827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="1141" mass="129994" checksum="667AF54BAE9CA625" modified="2024-05-29" version="1">MVDGDLVTGMKRSRDDDDAYSSPGIKRPFVSERQTFVVPIHLPTSLCNHQVLNDLGWTTLMTVNDASLRHVQDFSRHRQPPMTSKGSSQKMTANDVLTYIQKVKDIFHDKKETYDDFLKIMQDFKAQRLDTTGVIARVKELFDGHPELILGFNPFLPKGSEITLPREHGQGHFKKPLDFEEAIGFVNKIKMRFQGQDHVYKYFLDTLNKYKKENKSIKEVHQEVASLLNDQQDLLKEFTNFFPDSSAAGSNHSGHSSSNNNSVHDDRSSPSVTTRPLQSKKANASASCVESKDNVDRADAVHEKGRVVADKETDTSEDTELKEHNSNDKDCDGTHQHKPARTLEDSVAELFHQDLHGQMLCLREKVKERLSNSEDYQAFLKCIEQYCTEAITRPQLQSLVNNLLGAYADLVEEVNEFIDRSEMTRSGSKPSSLSSKDKYAAKSIQELDLSDCECCTPSYRLLPNDYPIPSASQKTKIGFEVLNDHWVSVTSGSEDYSFKHMRKNQYEESLFRCEDDRFELDMLVESVNVTAKRVEELLDRINDNSGKTDNSVHIEDFTAIHLKCIGRLYGDHGHDVLDVLRKNASLALPVILTRLKQKQEEWLRCRADNKKVWAEIYAKNYHKSLDHRSFYFKQQDSKSLSTKALLAEIKEMSEERSVEDNIRQCIAPGGKQNTIPHQEFKYSDLDIHEDLYQVMKYAVAQTGSPEQINKAVKIWTTFVEPILGVPPRSSRIADQNAAKTGNHAVTSGITAVKSTNRSRMLDDDSGGKENGFSNTDHSEHTGSESGDEEDLSPDADLDNNGESDDVAYGMSDEVYPIESLLERVKPVMLHVPAALHNKERMSRVFYGNDDFYVFFRLHQILYSRLEEAKEKSLVERWRGSNDSTPNDSYARFLDILYSFLDGAIDSAKYEDECRAVLGTWSFPVFTLDKLIDKLTKLLLAITMDEINNKLLDLYAYESLRKERFIDELYNANASVIVNDSKIFRFDCSSIPETHRQTRLTIQIINFGYEKSEPPAASLDPTFATYLNSQLLSVNPKKKRHRIFLKRNKQKYACEDVDLAMAKAMEKFHIVNGLEFKIAAFTHKVYYVMGTSDSMVQMCRKRINSRNGSGQSPENATVSNGRSFKALKFQKLLLSRLQPLEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C042</accession>
    <name>A0AAD5C042_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">Pyruvate kinase</fullName>
            <ecNumber evidence="4 14">2.7.1.40</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">M8C21_019882</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">AA19_3_7</strain>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>pyruvate + ATP = phosphoenolpyruvate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18157"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.40"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the pyruvate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.40" evidence="4 14"/>
    <dbReference type="EMBL" id="JAMZMK010010245">
        <property type="protein sequence ID" value="KAI7732404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C042"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030955">
        <property type="term" value="F:potassium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004743">
        <property type="term" value="F:pyruvate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.33.10:FF:000004">
        <property type="entry name" value="Pyruvate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="entry name" value="Phosphoenolpyruvate-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.33.10">
        <property type="entry name" value="PK beta-barrel domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1380.20">
        <property type="entry name" value="Pyruvate kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001697">
        <property type="entry name" value="Pyr_Knase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011037">
        <property type="entry name" value="Pyrv_Knase-like_insert_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015793">
        <property type="entry name" value="Pyrv_Knase_brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015795">
        <property type="entry name" value="Pyrv_Knase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036918">
        <property type="entry name" value="Pyrv_Knase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015806">
        <property type="entry name" value="Pyrv_Knase_insert_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01064">
        <property type="entry name" value="pyruv_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11817">
        <property type="entry name" value="PYRUVATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00224">
        <property type="entry name" value="PK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02887">
        <property type="entry name" value="PK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01050">
        <property type="entry name" value="PYRUVTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50800">
        <property type="entry name" value="PK beta-barrel domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52935">
        <property type="entry name" value="PK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11 14" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="8 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyruvate kinase barrel" evidence="15">
        <location>
            <begin position="30"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate kinase C-terminal" evidence="16">
        <location>
            <begin position="395"/>
            <end position="504"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF02887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAI7732404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="524" mass="56954" checksum="D83BD7518A90AE92" modified="2024-05-29" version="1">MHSNHLLLEEPIRMASILEPSKPSFFPAMTKIVGTLGPRSRSLDVISGCLTAGMSVARFDFSWGDCGYHQETLENLRAAVKSTKKLCAVMLDTVGAEMQVVNKSEKAISLQQDDVVILTPDEGQEASSQILPINFAGLAKAVKKGDTIFVGQYLFTGSETTSVWLEVDKVEGADVVCMIKNSATLAGALFTLHASQVHIDLPTLTDKDKENINTWGVRNKIDFLSLSYTRHAEDVREAREFLSKLGDLSQTQIFAKIENVEGLTHFDEILQEADGIILSRGNLGIDLPPEKVFLFQKAALYKCNMAGKPAVVTRVVDSMTDNLRPTRAEATDVANAVLDGSDAILLGAETLRGLYPVETISIVGKICAEAEKVFNQDMYFKKTVKYVGEPMTHLESIASSAVRAAIKVKASVIICFTSSGRAASGAFEETTAPSPPEGGWVVEEDIVYFRARQSLIVRGLFPMLADPRHPAESTSATNESVLKVALDHGKTSGVIKSHDRVVALDISPPRLQSLKSPKLLETLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C0Q2</accession>
    <name>A0AAD5C0Q2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_018571</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010359">
        <property type="protein sequence ID" value="KAI7731901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C0Q2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009506">
        <property type="term" value="C:plasmodesma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004714">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045272">
        <property type="entry name" value="ANXUR1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27003">
        <property type="entry name" value="OS07G0166700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27003:SF361">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="22"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7731901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="407" mass="46240" checksum="4977C1EC8C71C1AE" modified="2024-05-29" version="1">MSEMEKWKHLKIPFSDIKEATRNFQKKIGKGGYGSVYEGKLLVNGSDMKVAVKRSTAISHACGTLGYVEPELTQTSIATRKTDVYSFGMVLFEVLSGRLCNFKYEDGIILTAAVAKQYYASGDMDRIIDPVLLGQMSPNSLRKFSAVAIDCLKAREQRPLMDVVKKELEESLQIQVTHLTPRNSMPGTNLLRIPFTEIKDATKGFQNPIIEGDYGFMYEGELSIHGIYTKVMIKRSSMEFGQGIEEFVTEIQMLSAIISDTDVYDDPNTSTIVETKKADVYSFGVVLFEVLCGRLCNVKYDDGIMLSTKTAKEYYAKGKMDKLIDPILREQMRIDSLRKLSAIAYECLQDLEQRPFIDVVKIKLEEMLKNEVLHWRGFFSRHWYILDVEGILSPETHIEVSVDCRIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C2R4</accession>
    <name>A0AAD5C2R4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_026544</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009848">
        <property type="protein sequence ID" value="KAI7734027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C2R4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22157">
        <property type="entry name" value="F-box_AtFBW1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013187">
        <property type="entry name" value="F-box-assoc_dom_typ3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017451">
        <property type="entry name" value="F-box-assoc_interact_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01640">
        <property type="entry name" value="F_box_assoc_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31111">
        <property type="entry name" value="BNAA05G37150D PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31111:SF125">
        <property type="entry name" value="F-BOX PROTEIN CPR30-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08268">
        <property type="entry name" value="FBA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="12"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7734027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="385" mass="44367" checksum="4195B9FE03B02234" modified="2024-05-29" version="1">METIEHKQADDDDSKISYPEEIIEQIISRLPIKSILRFKSISKPWLSLISNPSFTKLHSTVGPSTTSLFLTLVDSSTNTRYFFSGANNDNGSVANLIKLDNTPYVFGTVEHLNGLLCLIDRDYHSDNIYASVINPTTHKFFKLPDRPLVPLLNEDMCYFFGFDESSNEHKVLYVDVGLIKLTTIEIMIFSFLNYSWRKINVDLPIGVSRDRWFYGTKVSVCVNSIIHMMIRNPFEILAFDLRTETFSIVNVPPNPMVQNIYTFVCPYHRFLKINGLLGIAVIGNYLEEIDKVDIWILQDYENRVWVRETVTFPPNSQLCYGVGSPYPFDSINMDEIIFYPRVLSENLISVPIYNMKTRCFKSIQLTLGHRFLCPESVEVEEIKLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C3C4</accession>
    <name>A0AAD5C3C4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">Pyruvate kinase</fullName>
            <ecNumber evidence="4 14">2.7.1.40</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">M8C21_003911</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">AA19_3_7</strain>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>pyruvate + ATP = phosphoenolpyruvate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18157"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.40"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the pyruvate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.40" evidence="4 14"/>
    <dbReference type="EMBL" id="JAMZMK010009754">
        <property type="protein sequence ID" value="KAI7734347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C3C4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030955">
        <property type="term" value="F:potassium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004743">
        <property type="term" value="F:pyruvate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.33.10:FF:000004">
        <property type="entry name" value="Pyruvate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1380.20:FF:000006">
        <property type="entry name" value="Pyruvate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="entry name" value="Phosphoenolpyruvate-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.33.10">
        <property type="entry name" value="PK beta-barrel domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1380.20">
        <property type="entry name" value="Pyruvate kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001697">
        <property type="entry name" value="Pyr_Knase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011037">
        <property type="entry name" value="Pyrv_Knase-like_insert_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015793">
        <property type="entry name" value="Pyrv_Knase_brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015795">
        <property type="entry name" value="Pyrv_Knase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036918">
        <property type="entry name" value="Pyrv_Knase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015806">
        <property type="entry name" value="Pyrv_Knase_insert_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11817">
        <property type="entry name" value="PYRUVATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00224">
        <property type="entry name" value="PK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02887">
        <property type="entry name" value="PK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01050">
        <property type="entry name" value="PYRUVTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50800">
        <property type="entry name" value="PK beta-barrel domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52935">
        <property type="entry name" value="PK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11 14" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="8 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyruvate kinase barrel" evidence="15">
        <location>
            <begin position="3"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate kinase C-terminal" evidence="16">
        <location>
            <begin position="328"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="17">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF02887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAI7734347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="460" mass="50182" checksum="390C97475A1CF7A2" modified="2024-05-29" version="1" fragment="single">CVVARFDFTWGDAEFHQETLENLKIAIKKTKKLCSVMLDTTGPELLVVNKADHPIPLEAESFVVLTPDQEKEATSDLLPVNFGGLAKAVKPGDTIFLGQYLFTGSETTSVWLEVSEVKGDDVVCLIKNSAVLAGTLYTLHVSQVISTWGVKNNVDFLSLSHTRSGQDIREAREYLSKLGDLSQTQIFAKIENVEGLVNFDEILQEADGIILARGNLGIDLPPEKVFLFQKAAVYKCNMAGKPAVVTRVVDSMTDNLRPTRAEATDVANAVLDGADAILLGAETMRGLYPVETISIVGKICGESEKVYNQDSYFKNTVKYVGEPMSHMESIASSAVRAAIKVKASVIICFTSSGRAARLIAKYRPTMPVLSVVIPRLKTNQLRWTFTGGFEARQSLIIRGLFPMLADPQHPGGSKSATNETVLKVALDHGRNIGVIKSHDRVVVCQKVGDDSVVKIIELQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C3K6</accession>
    <name>A0AAD5C3K6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">NADH:quinone oxidoreductase/Mrp antiporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_033109</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a plastoquinone + NADH + (n+1) H(+)(in) = a plastoquinol + NAD(+) + n H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:42608"/>
            <dbReference type="Rhea" id="RHEA-COMP:9561"/>
            <dbReference type="Rhea" id="RHEA-COMP:9562"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17757"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:62192"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a plastoquinone + NADPH + (n+1) H(+)(in) = a plastoquinol + NADP(+) + n H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:42612"/>
            <dbReference type="Rhea" id="RHEA-COMP:9561"/>
            <dbReference type="Rhea" id="RHEA-COMP:9562"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17757"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:62192"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast thylakoid membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009658">
        <property type="protein sequence ID" value="KAI7734733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C3K6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003954">
        <property type="term" value="F:NADH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048039">
        <property type="term" value="F:ubiquinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015990">
        <property type="term" value="P:electron transport coupled proton transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003918">
        <property type="entry name" value="NADH_UbQ_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001750">
        <property type="entry name" value="ND/Mrp_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43507:SF21">
        <property type="entry name" value="NAD(P)H-QUINONE OXIDOREDUCTASE CHAIN 4, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43507">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00361">
        <property type="entry name" value="Proton_antipo_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01437">
        <property type="entry name" value="NUOXDRDTASE4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="2" id="KW-0934">Plastid</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-1278">Translocase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="121"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="171"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="206"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="NADH:quinone oxidoreductase/Mrp antiporter transmembrane" evidence="8">
        <location>
            <begin position="11"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7734733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="282" mass="31558" checksum="9E6E62E68CDAFC0D" modified="2024-05-29" version="1" fragment="single">AKLVFLFRKIIFYIGFFVAFAVKLPILPLHTWLPDTHGKVHYSTCMLLAGILLKMGAYGLIRINMELLPHAHSIFSPWLMIVGTIQIVYAASTSPGQSYSSVSHMGFIRIASITHMRLNGAILQIISHGFIGAALVFLAGTSYDRIRLVYLDEMGGVAIPMPKIFTTFSRFSMASLALPGMRGFVAEVLVFLGIITSQKYLLMPKIAITFVMAIGMILTPIYLLSMSCRLFYGYKLFNTPNSYVFFILDHASIGMYPDFVLSLSVDKVEDSKKKKDFYSLNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C4H7</accession>
    <name>A0AAD5C4H7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FHA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_024599</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009531">
        <property type="protein sequence ID" value="KAI7735211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C4H7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.20:FF:000028">
        <property type="entry name" value="FHA domain-containing protein DDL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050923">
        <property type="entry name" value="Cell_Proc_Reg/RNA_Proc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23308">
        <property type="entry name" value="NUCLEAR INHIBITOR OF PROTEIN PHOSPHATASE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FHA" evidence="2">
        <location>
            <begin position="324"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="106"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="163"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="207"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="218"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7735211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="419" mass="48472" checksum="3006CB66E0B2ACA1" modified="2024-05-29" version="1" fragment="single">VIQPASRRYLMGRESYDRSESPVRNHRSSHRSPPRRERSPARHRSSHSTKSPPREKPSSRTRSPKRPKSPISHSPLRDKPSSHNKSSRHTRSRSPSDRLPAKEPALRSPGSRSPSPRSKRLRRDRSDHKDDKLSERVPEKNHRKTRSSESRSPSPRTRRLRRAQPDSGEKVSDRGVRKEKDLERERRDRSSGRDEDGGGSYKSRQVEHHRTRRRSRSPPRDEVSGDIHSCERGNNSREDHRNGDDDSVARMKAAEEALETKEKQKPSFELSGKLAAETNRVKGVTLLFTEPPDARKPDIRWRLYVFKGGEVLNEPLYVHRQTCYLFGRERRVADIPTDHPSCSKQHAVLQYRQVEKELPDGMLGKEVKPYIMDLGSTNGTFINDNRIEPERYYELMEQDTIKFGNSSREYVLLHENSAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C4T8</accession>
    <name>A0AAD5C4T8_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_014850</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009847">
        <property type="protein sequence ID" value="KAI7734046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C4T8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000121">
        <property type="entry name" value="Myb transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015495">
        <property type="entry name" value="Myb_TF_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47999">
        <property type="entry name" value="TRANSCRIPTION FACTOR MYB8-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47999:SF9">
        <property type="entry name" value="TRANSCRIPTION REPRESSOR MYB5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="8">
        <location>
            <begin position="9"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="7">
        <location>
            <begin position="9"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="8">
        <location>
            <begin position="62"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="7">
        <location>
            <begin position="62"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="102"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="133"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7734046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="247" mass="27786" checksum="ED572DA61E9161D4" modified="2024-05-29" version="1">MGRAPCCAKVGLHRGAWSDEEDKLLTNYIQTHGEGQWRSLPSKAGLLRCGKSCRLRWMNYLRPGIKRGSFTADEDDTIIRLHSVHGNRWSFIATELPGRTDNEIKNHWNSHLKRKTIEGGSDNQPKKKKKKKTTEQKKVMKTVEKPVETCSSHESVSLTLRKNDSFDNGVMSGASSSCCTVDQGEADFPWPDWSPLFEMEGSGSMFVDDPSLSFDGCDLLNSCDDDSQMLDRLYSEYLNLLEDGEGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C6F4</accession>
    <name>A0AAD5C6F4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Tetraspanin-6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_022375</name>
        <name evidence="8" type="ORF">M8C21_022379</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tetraspanin (TM4SF) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009398">
        <property type="protein sequence ID" value="KAI7735963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAMZMK010009398">
        <property type="protein sequence ID" value="KAI7735967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C6F4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009734">
        <property type="term" value="P:auxin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044991">
        <property type="entry name" value="TET_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32191">
        <property type="entry name" value="TETRASPANIN-8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00259">
        <property type="entry name" value="TMFOUR"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="43"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="74"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7735963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7735967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="226" mass="25327" checksum="3F15B1C78E2D9C6F" modified="2024-05-29" version="1" fragment="single">MSRVSNTLIGFLNLLTLLVSIPIIGGGLWMAKSTATCESFLQRPLMVVGFVILLVSLAGFIGACFHVVWALWLYLAVMLFLIAALLAVTVFGFVVVGPGGGVDVPGRVYKEYHLQSYSPWLRKRIENPTYWMTVKSCILGSKTCNKIVMWTPADYWTRNLSPIQSGCCKPPTACDYQAPMMTQDPDCYKWNNDPNLLCYDCDSCKAGVLEDVRRDWHRLSILNIIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C6T3</accession>
    <name>A0AAD5C6T3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_025937</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009574">
        <property type="protein sequence ID" value="KAI7735051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C6T3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7735051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="219" mass="24294" checksum="A7DDD923047EEBC3" modified="2024-05-29" version="1" fragment="single">AIGEVASVFLTAAIAIQECLIHLSSLALRALGFAYKEDQPEFTTYNGDEDHPVHNQFRKSHGKIDENKVYNWKRKRKIDRLVVTEEAIDIRNDPQFLSYQSLFGIVKANVSLVEGRTLNRFGGKGCSVLGRVRIHDSFVVKDPLIRKFVKPLNTAEQQESSYCHKPEITRTQLKKLKDLGEVIAMTGDGVNDAAALKLAEIGKVVKEASDMVVAAVGEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C6V0</accession>
    <name>A0AAD5C6V0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Bulb-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_022386</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009398">
        <property type="protein sequence ID" value="KAI7735974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C6V0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048544">
        <property type="term" value="P:recognition of pollen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00028">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.90.10.10">
        <property type="entry name" value="Bulb-type lectin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001480">
        <property type="entry name" value="Bulb-type_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036426">
        <property type="entry name" value="Bulb-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000858">
        <property type="entry name" value="S_locus_glycoprot_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32444">
        <property type="entry name" value="BULB-TYPE LECTIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32444:SF235">
        <property type="entry name" value="OS01G0783900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01453">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00954">
        <property type="entry name" value="S_locus_glycop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00108">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51110">
        <property type="entry name" value="alpha-D-mannose-specific plant lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50927">
        <property type="entry name" value="BULB_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042208751" description="Bulb-type lectin domain-containing protein" evidence="4">
        <location>
            <begin position="24"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="Bulb-type lectin" evidence="5">
        <location>
            <begin position="24"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7735974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="316" mass="35419" checksum="68B63CA3F9CB21E0" modified="2024-05-29" version="1">MRTLTKLLFFSIALFSALSNSAAVDTITGGQALIDNDTIVSSNEMYELGFFSPGNSKNRYLGIWFKKIYPQTVVWVANRETPLTGTKGVLRVNSNGMLLLFSGNNTMICSTDQDFIWQSFDYPTDTLLAGMKFGKDLVTGLDRHLTSWKSMDDPSPGLYVSWMDTNGYPQAFERRGSVLRLRFGPWTGVSSIMSRVYLTPEGNEMRLNYIDPTQGWMPYLVTTVDMCTQYGLCGAYGICNINTSPACTCTEGFQPRYPVQWNAGNWSGGCKHEKPLNCGNANGFKKLSGVKLPDTRHSWYDQLNKKLQPLQEHSSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C7W4</accession>
    <name>A0AAD5C7W4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">X8 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_030769</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009463">
        <property type="protein sequence ID" value="KAI7735606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C7W4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009506">
        <property type="term" value="C:plasmodesma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1040:FF:000001">
        <property type="entry name" value="Glucan endo-1,3-beta-glucosidase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012946">
        <property type="entry name" value="X8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044788">
        <property type="entry name" value="X8_dom_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31044">
        <property type="entry name" value="BETA-1,3 GLUCANASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31044:SF28">
        <property type="entry name" value="CARBOHYDRATE-BINDING X8 DOMAIN SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07983">
        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00768">
        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="X8" evidence="9">
        <location>
            <begin position="40"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7735606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="134" mass="14241" checksum="28230E329004C18E" modified="2024-05-29" version="1">MFSFPSPTGFIPTPPLFEPPVVYPPPNGPPPPHSEAASALWCVAKPSVPDPIIQEAMTYACGSGADCESLQPNGDCFQPDTLFAHASYAFNSYWQRTKVAGGTCEFGGSAMLVTVDPSKAIISQPIPHQILTFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C7X5</accession>
    <name>A0AAD5C7X5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">WEB family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_022332</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WEB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009459">
        <property type="protein sequence ID" value="KAI7735621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C7X5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009904">
        <property type="term" value="P:chloroplast accumulation movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009903">
        <property type="term" value="P:chloroplast avoidance movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008545">
        <property type="entry name" value="Web"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32054">
        <property type="entry name" value="HEAVY CHAIN, PUTATIVE, EXPRESSED-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32054:SF4">
        <property type="entry name" value="OS07G0677900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05701">
        <property type="entry name" value="WEMBL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="386"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="76"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="14"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="398"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7735621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="531" mass="58647" checksum="0C8A35C7ACE07699" modified="2024-05-29" version="1">MESLDSDPNIGSGFIQTGSDPKQSNGGGGTRGEVDTSAPFESVKEAVTRFGGVGFWKPHSKNLIASENGTAEEFDAAKAEEQAAQLANDLMVKERETLEVLKELEATKSLVEELKVKLQKESAAVNDQLKEVKESRDEHDENALELKSAGDSLMSSSSSSAPGFILMELKQAKLNLTRTTSDLADIRATVETYSKMIEKERFELEKTRQRISTNCSKILSLKDVDFPKELHRLTSETEQFKKVGEVAKSEVLRSMNQIKQTKRKIKTAEIRLIAARKLKEAARASEALARIEMKSISESQTLAEGDGVTLSFEEYNCLKLKAQEAEESLVKKVNESVVKDNQSEILNRVEEATEEVKHSKRALEEALSKAEAANNDKLKAEEALRKWRSDHGQRRKSTVQNSTKFKNSSSSRNSTRMLDVNEAHLGGNVTNPMLAGPILAPTMSIGQILSRKLLLTEEYYEKSDMKRKVSLAQMLSKPSIIGGGNGGGGVDGCRGAKGKRKKFGFGRIQFLVTKPSKKKKKHTVSPPRLND</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C9E9</accession>
    <name>A0AAD5C9E9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">dolichyl-diphosphooligosaccharide--protein glycotransferase</fullName>
            <ecNumber evidence="7">2.4.99.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">M8C21_024754</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="22">AA19_3_7</strain>
            <tissue evidence="22">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>a di-trans,poly-cis-dolichyl diphosphooligosaccharide + L-asparaginyl-[protein] = N(4)-(oligosaccharide-(1-&gt;4)-N-acetyl-beta-D-glucosaminyl-(1-&gt;4)-N-acetyl-beta-D-glucosaminyl)-L-asparaginyl-[protein] + a di-trans,poly-cis-dolichyl diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22980"/>
            <dbReference type="Rhea" id="RHEA-COMP:12804"/>
            <dbReference type="Rhea" id="RHEA-COMP:12805"/>
            <dbReference type="Rhea" id="RHEA-COMP:19506"/>
            <dbReference type="Rhea" id="RHEA-COMP:19509"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:50347"/>
            <dbReference type="ChEBI" id="CHEBI:57497"/>
            <dbReference type="ChEBI" id="CHEBI:57570"/>
            <dbReference type="ChEBI" id="CHEBI:132529"/>
            <dbReference type="EC" id="2.4.99.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the oligosaccharyltransferase (OST) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the STT3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.99.18" evidence="7"/>
    <dbReference type="EMBL" id="JAMZMK010009130">
        <property type="protein sequence ID" value="KAI7737063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C9E9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004579">
        <property type="term" value="F:dolichyl-diphosphooligosaccharide-protein glycotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12610:FF:000002">
        <property type="entry name" value="dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003674">
        <property type="entry name" value="Oligo_trans_STT3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048999">
        <property type="entry name" value="STT3-PglB_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048307">
        <property type="entry name" value="STT3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13872">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13872:SF1">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT STT3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02516">
        <property type="entry name" value="STT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21436">
        <property type="entry name" value="STT3-PglB_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="17" id="KW-0464">Manganese</keyword>
    <keyword evidence="15 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="10 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="105"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="137"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="163"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="193"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="224"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="258"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="290"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="320"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="383"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="408"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="domain" description="Oligosaccharyl transferase STT3 N-terminal" evidence="20">
        <location>
            <begin position="42"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="domain" description="STT3/PglB/AglB core" evidence="21">
        <location>
            <begin position="557"/>
            <end position="615"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF02516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF21436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="KAI7737063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="734" mass="82758" checksum="1FCC30BAAC6C27F0" modified="2024-05-29" version="1">MVSKSDTMKPTHQPDLSSKPPLPFSFKSFKLKTKQQELLIRVSILCLVYILAFITRLFSVLRYESMIHEFDPYFNYRTTLYLTEKGFYEFWNWFDSESWYPLGRIIGGTLYPGLMVTAALIYWILLFLRFTVHIREVCVLTAPFFASNTTIVAYLFGKEVWDTGAGLVAAALIAICPGYISRSVAGSYDNEGVAIFALLLTFYLFVKAVNTGSLAWALGSAFGYFYMVSAWGGYVFIINLIPLYVLVLLVVGRYSMRLYVAYNCMYIVGMLLAMQIRFVGFQHVQSGEHMAAMGVFFLIQVFYFLAWVKHLLNDTKLFQALLRIGVTSAVGVGAIALGLGTASGYISPWTGRFYSLLDPTYAKDHIPIIASVSEHQPTAWSSFMFDFHILLFLFPAGLYFCFKRLSDATIFIVMYGLTSMYFAGVMVRLILVATPAVCLISAIAVSATIKNLTQIIRGKTGQTVSAKGTSTAKGSSKQQASVDQSIPFLKNGATILLFGAFYLLSRYAIHCTWVTSEAYSSPSIVLAARGAHGQRVIFDDYREAYFWLRQNTPSDAKIMSWWDYGYQITAMGNRTVIVDNNTWNNTHIATVGRAMSSYEDEAYEIMRSLDVDYVLVVFGGVTGYSSDDINKFLWMVRIGGGVFPVIKEPDYLVNGEYRVDKGAAPKMLNCLMYKLCYYRFGELTTEYGKPPGYDRARGVEIGNKDVKLEYLEEAFTTSNWIVRIYKVKPPTNRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CB98</accession>
    <name>A0AAD5CB98_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">HSF-type DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_014435</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Homotrimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the HSF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008838">
        <property type="protein sequence ID" value="KAI7738269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CB98"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000037">
        <property type="entry name" value="Heat stress transcription factor B-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000232">
        <property type="entry name" value="HSF_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015">
        <property type="entry name" value="HEAT SHOCK TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015:SF337">
        <property type="entry name" value="HEAT STRESS TRANSCRIPTION FACTOR A-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00447">
        <property type="entry name" value="HSF_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00056">
        <property type="entry name" value="HSFDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00415">
        <property type="entry name" value="HSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00434">
        <property type="entry name" value="HSF_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0346">Stress response</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HSF-type DNA-binding" evidence="12">
        <location>
            <begin position="56"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="203"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="117"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="210"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7738269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="253" mass="29240" checksum="5BE309DD59848273" modified="2024-05-29" version="1">MVPKPLDCLQGTQIPAFLSKTFDLVNDTTLDPIVSWHRSGQSFVIWDPVTFARMLLPKNFKHNNFSSFVRQLNSYGFRKIDPDKWEFANESFLRGHKHLLKNIQRRRSNQSMSSSSSLLANEEFNNLEAEIQRLHKEKTEMMQQVVELKNENCVTHQYMESINKKLKAAENKQKQMLLFMAKMFKNPTEQHEQMLRISSARTARKFGKHQPHEEGDSRGKNVLETDQSEAAPDHLLEPLSGGIDGITAKQEDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CD39</accession>
    <name>A0AAD5CD39_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">MHD2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_031821</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008695">
        <property type="protein sequence ID" value="KAI7738935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CD39"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014772">
        <property type="entry name" value="Munc13_dom-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057984">
        <property type="entry name" value="PATROL1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008528">
        <property type="entry name" value="unc-13_homologue"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31280">
        <property type="entry name" value="PROTEIN UNC-13 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31280:SF2">
        <property type="entry name" value="PROTEIN UNC-13 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25761">
        <property type="entry name" value="TPR_PATROL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51259">
        <property type="entry name" value="MHD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MHD2" evidence="2">
        <location>
            <begin position="772"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="150"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="169"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="197"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="934"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7738935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="934" mass="106357" checksum="B254E4A368751CD6" modified="2024-05-29" version="1" fragment="single">SKAFENISLHFDSHSSHSNFTYHRSRFLKINLENEVELLQRYRRDRRILLNYLLSGSLIKKVVMPPGAVSLDDVDLDQELLLLNEIATVSKFRLRSKVYERWKELGLNTKTKCGSLKRGEMLELSEAIRDYHDSTGLPQMNAESEGEFFLATNPESSGSPPRRAPPAVPFVVPSPIVPDLTSSNPFDANEEEDEPDIISNSQSINSGQDRNLTVDDIDDFEDEDDLEELTSRRYSRRTLNDTADLVVGLPSFATGVNDDDLRETAYEILLAAAGASGGLIVPSKEKKKEKKSRLLKKLGRSKSEQVTAQSQQSTGLTGLLETMRVQMEISEAMDIRTRQGLLNAMAGKVGKRMDALLIPLELLSCVSRTEFSDKKAYIRWQKRQAIQLNMLEEGLVNHPVVGFGESGRKASEMRVLLARIEESESFAPSVGELQRIECLRSLREIAIPLAERPARGDLTGEVCHWADGYHLNVRLYEKLLLSVFDVLDEGKLTECHIKSNIFGSLNNLQVRKVSRYCEVEEILELLKSTWRILGITETIHHTCYAWVLFRQFVMTREHGLLRLAIEQLNKVPMKEERGPQERMHLKGLYCKVENEEGSRELTFLQSFLMPIQKWADRQLADYHLHFSEQGDFFRRNLDRRESGIKAFVKKELNDPRLPDMRKSRDINILTTPTLCVQLNTLYVSGNIIVKFYENYGISHLNKLEDSIWERWTRKTKHGKSNNENSKKESFDGSRKDINAAIDRICEFTGTKIIFWDLREPFIENLYRPSVSESRLETLIEPLDVELNQLCDVIVEPLRDRIVTSLLQASLDGLLRVLLDGGPSRLFIPSDAKSLEEDLEVLKEFFISGGDGLPRGVVENQVARVRQVIKLQGYEGGKWAGHTRELIDDLKSASASDRSKLGADTKTLLRILCHRCDSEASQFLKKQYKIPKSAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CE96</accession>
    <name>A0AAD5CE96_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 15">Histone acetyltransferase</fullName>
            <ecNumber evidence="3 15">2.3.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">M8C21_020870</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">AA19_3_7</strain>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-lysyl-[protein] + acetyl-CoA = N(6)-acetyl-L-lysyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45948"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the MYST (SAS/MOZ) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="3 15"/>
    <dbReference type="EMBL" id="JAMZMK010008558">
        <property type="protein sequence ID" value="KAI7739859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CE96"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004402">
        <property type="term" value="F:histone acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18642">
        <property type="entry name" value="CBD_MOF_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000067">
        <property type="entry name" value="Histone acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.60:FF:000001">
        <property type="entry name" value="Histone acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000002">
        <property type="entry name" value="Histone acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.60">
        <property type="entry name" value="N-acetyl transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002717">
        <property type="entry name" value="HAT_MYST-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050603">
        <property type="entry name" value="MYST_HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025995">
        <property type="entry name" value="Tudor-knot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040706">
        <property type="entry name" value="Zf-MYST"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10615">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10615:SF161">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE KAT7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01853">
        <property type="entry name" value="MOZ_SAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11717">
        <property type="entry name" value="Tudor-knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17772">
        <property type="entry name" value="zf-MYST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51726">
        <property type="entry name" value="MYST_HAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0007">Acetylation</keyword>
    <keyword evidence="11" id="KW-0010">Activator</keyword>
    <keyword evidence="14" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13 15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MYST-type HAT" evidence="16">
        <location>
            <begin position="152"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="17">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU361211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAI7739859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="319" mass="37841" checksum="0F0A56BB3C6F9F9D" modified="2024-05-29" version="1" fragment="single">TIYVTPPEQELNPGISIKAKEINPLAVFVVRIELNRMGVMQALEVGTRVLCRWRDGKHHPVKVIERRRLQSGGPNDYEYYVHYTEFNRRLDEWVRLEQLDLDSVENDVDEKVEDKVTGLKMTRHQKRKIDETHVEGHEELDAASLREHEEFTKVKNIATIELGKYEIETWYFSPFPAEYNDCLNLYFCEFCLTFMKRKQQLQRHMKKCDLKHPPGDEIYRSGTLSMFEVDGKKNKVYGQNLCYLAKLFLDHKTLYYDVDLFLFYVLCECDDRGCHMVGYFSKEKHSEESYNLACILTLPPYQRKGYGKFLIAFFFDMFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CEE4</accession>
    <name>A0AAD5CEE4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FBD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_011415</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008525">
        <property type="protein sequence ID" value="KAI7740077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CEE4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006566">
        <property type="entry name" value="FBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31639:SF326">
        <property type="entry name" value="F-BOX DOMAIN, FBD DOMAIN, F-BOX-LIKE DOMAIN SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31639">
        <property type="entry name" value="F-BOX PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08387">
        <property type="entry name" value="FBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00579">
        <property type="entry name" value="FBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FBD" evidence="1">
        <location>
            <begin position="306"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7740077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="379" mass="43861" checksum="8B008F6C9013AF5E" modified="2024-05-29" version="1">MEAEGLPLDRISSLPPSIIHLILIRMPVRDAFRTSILSQNWRHHCRYIPTLWFDNFHGPYDETLSNKCTMLHSVVYPILLLHRGPILQFILYLYGVNSCCEIDQIILHLSRNAALKDFRLWICSGDEHKLLPAFFKLQELMVLELRNCAFQPPVTFKGFSMLVKLYFYEVSIAAKDFLRIISNCPLLKDFTLIADEKHLLGLWSFNFIELFERLPLLEHLCMSSYPIQCFAKGVIRQKLPISLGRLKSLEFYGFGLCFGREVDLLSVLLLVTSSPNIETINMEMELYPTEAVSQTTMNLVDHQDYSYVMLDHLRVVVISSFSNMKTSMDFVKLILAKSPMLKKVVIFINEQVDIHGKVKILSEMIRYPRASAKAEIRVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CG95</accession>
    <name>A0AAD5CG95_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_031079</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008264">
        <property type="protein sequence ID" value="KAI7741143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CG95"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11453">
        <property type="entry name" value="bHLH_AtBIM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044295">
        <property type="entry name" value="BIM1/2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46412">
        <property type="entry name" value="BES1-INTERACTING MYC-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46412:SF3">
        <property type="entry name" value="TRANSCRIPTION FACTOR BIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="6">
        <location>
            <begin position="240"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="100"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="157"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="222"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="24"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="161"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="173"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="222"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="246"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7741143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="393" mass="43713" checksum="661E609FBAABAF1F" modified="2024-05-29" version="1">MELSQPRPYGTQGRKPTHNFLSLYASSQQDQPSTPQGGFLETHDFLQPLERVGKNVDKEPTTAVEHILPGGIGTYSISHISCISQSQRVAKAEGGVIRSVQSSGSDKNDENSNCSSQTGSGFSLWEEPTLKMGKTGKENIAGNTCVIREGVAKTGGVSWMTSTERPSESTSSGHNYPTVTKSLSSSRPPSGQNNPCFVDMFKYAKSSQDDEDDEEHEFVIKQEPSSHYKVDTHHDQKPNTPRSKHSATEQRRRSKINDRFSILKELIPHGDQKRDKASFLLEVIEYIRYLQEKAHKYEDSCRNNNNNSKNQRPIEGFIDQPLVNKGNVLLNVSKKGQTLIDSDLSAHERVDHNLSLTNKGSYTNYCPASDEVKDEEVTIESGTISISTVYSQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CGH4</accession>
    <name>A0AAD5CGH4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">UDP-glycosyltransferases domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_009360</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008222">
        <property type="protein sequence ID" value="KAI7741293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CGH4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080043">
        <property type="term" value="F:quercetin 3-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080044">
        <property type="term" value="F:quercetin 7-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000027">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926">
        <property type="entry name" value="GLUCOSYL/GLUCURONOSYL TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926:SF1498">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7741293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="308" mass="34840" checksum="604C7385414D91D8" modified="2024-05-29" version="1">MSYETNGYLETSLDWIPGMNNIRLRDFPSFIRTTNINDIMLNYLVTEAEALPRASAVVLNTFDDLEQDTVDMLTASMPQIFTVGPLNLMQQHLQDERVIQLGSSLWKEDVSCIHWLNSKDPGSVIFVNFGSIAVLTKEQLIEFGWGLANSKKDFLWITRPDIVGGNEAVLTPDFLDETKGRSLIISWCPQEQVLKHPAIGGFLTHCGWNSTLESISSGVPMLCWPFFAEQQTNCRYSCVEWGIGMEIDTNVKREEVEAQVKEMIEGAKGKMMKAKAMELKRKSEEAVTIQGSSRLNFEKFINEVLSKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CGH8</accession>
    <name>A0AAD5CGH8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GDSL esterase/lipase 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_019351</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008252">
        <property type="protein sequence ID" value="KAI7741172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CGH8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016298">
        <property type="term" value="F:lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01837">
        <property type="entry name" value="SGNH_plant_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044552">
        <property type="entry name" value="GLIP1-5/GLL25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008265">
        <property type="entry name" value="Lipase_GDSL_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035669">
        <property type="entry name" value="SGNH_plant_lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45966:SF4">
        <property type="entry name" value="GDSL ESTERASE_LIPASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45966">
        <property type="entry name" value="GDSL-LIKE LIPASE/ACYLHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01098">
        <property type="entry name" value="LIPASE_GDSL_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7741172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="350" mass="39087" checksum="37A3BE3E69604C23" modified="2024-05-29" version="1">MLLTINHVSAAGVFVFGDSIFDAGNNNYINTTSLDQSNFPPYGQTYFGFPTGRFSDGRIIPDFICEHASLPLIPPYLDPMSHAFFHIGANFASAGAGALLQTFQGSVISLQTQLRYHKRVEKVLRKMYGNTKANTILSKAVYLFSIGSNDYTSPYLITNSTHFNSSYSNAHLVRIVIGNLTTAIKDLHHRGARKFGFLNLGPLGCSPGLRIILNPSNDSGGCVQSASLLATMHNKALAKSLKILAKQLHGFRYSLYDFHHNLNQRLKHPSRYGYKQGKTACCGTGRFRGTFSCGGKRPVKEYWVCENPKEYVFWDSYHLTERVHKQMAHQMWNQQPTTGTSNLKTLFQSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CH39</accession>
    <name>A0AAD5CH39_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_021328</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SAPS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008440">
        <property type="protein sequence ID" value="KAI7740419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CH39"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019903">
        <property type="term" value="F:protein phosphatase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007587">
        <property type="entry name" value="SAPS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12634:SF37">
        <property type="entry name" value="SIT4 PHOSPHATASE-ASSOCIATED FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12634">
        <property type="entry name" value="SIT4 YEAST -ASSOCIATING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7740419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="125" mass="13915" checksum="A9868FE21042BC66" modified="2024-05-29" version="1">MLWRIPFIACEIFTCEVDIILKALVEDDESMNLLFSFLDPEHLHSTQLAGYFSYCMPAIAKDSSLNELFSGGEANLTLVDIVIEGGAVVLTLKDQNVLADGDINEGVDMLENVEIGEQKRRNEAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CJ50</accession>
    <name>A0AAD5CJ50_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sulfotransferase</fullName>
            <ecNumber evidence="3">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_012389</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the sulfotransferase 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010007890">
        <property type="protein sequence ID" value="KAI7742802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CJ50"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11783">
        <property type="entry name" value="SULFOTRANSFERASE SULT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sulfotransferase" evidence="4">
        <location>
            <begin position="66"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7742802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="329" mass="38075" checksum="5953DA0DF6AC2564" modified="2024-05-29" version="1">METPHPQEPKYLKDDEVSQECMSLLSTLPSESGWLSKKLYKYQGFWHNSRQLQGVISCQKHFQPQDTDILLVTTPKSGTTWLKAILFSLVNRTHFKPTKGDHPLLTNNPHTLVPFLELKLYVDGQTPDLSSFASPRLFSTHLPYVSLPKSIQDSKCKLVYLCRNPKDIFISLWAFTNKLRLQEMGTNSIEQVFEKFCEGVSLYGPFWDHMLSYWNESLKNPDKIYFLTYEEMKEQPNLHLRKLADFLGCPFSTKEVEENMVDDILDICSFDNMSNLKVNKEGKLPSGESNNAFFRNGKVGDWKNYLTMEMVERLEKICEEKLVGSGLKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CJR3</accession>
    <name>A0AAD5CJR3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transcription initiation factor TFIID component TAF4 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_007256</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">TAFs are components of the transcription factor IID (TFIID) complex that is essential for mediating regulation of RNA polymerase transcription.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008074">
        <property type="protein sequence ID" value="KAI7742115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CJR3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08045">
        <property type="entry name" value="HFD_TAF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000015">
        <property type="entry name" value="Transcription initiation factor TFIID subunit 4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045144">
        <property type="entry name" value="TAF4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007900">
        <property type="entry name" value="TAF4_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15138">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15138:SF14">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05236">
        <property type="entry name" value="TAF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription initiation factor TFIID component TAF4 C-terminal" evidence="8">
        <location>
            <begin position="267"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="140"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="215"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="389"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="431"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="14"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="149"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="215"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="389"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="464"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="477"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00058775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF05236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7742115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="525" mass="57737" checksum="3EB15E3F943BBA99" modified="2024-05-29" version="1">MNLNRFSHLAQLSVKRNTQKSSASVATSSIRRSSDSNTRPTENKSQKTQLMEHQSDSRGVQHMGFPQSSFSTYRYPSGNYHTGASPNTNMSSVSLQTRQGPVHPVRMHGGGSTHFISSLPNLSRWQPSMHENQSLMSSMAYGGPDHMDQTNGQQQKSQVSASQGPSSFLQASGVPGSSKDNTFDMLSSMHGFSKPVDKPGLRSITAQLELQHASAGNLSSEAGSNAKTPPKKATVAQKKPLEVPHVSSLSKRQKVCGAFSDQSIEPFNDVTAVSGVDLREEEEQLFSGFNEENRVSEDARKLVQEEEERLFLQKVPLQKKVAEIMSKCGVKNKSNDVERCISWCVKERMHGLITNLTRVSKQRVDIEKPRHRTVITSDIRQQIMSLNQKAREECDEKQVNKVEDDKSRTADANVAARAALGGHDMLSKWQLKAEQARQKRKGGPGAVSASKPSLDVGFGSKPEPTSEKNSTKDNPERTSGSVTTTSGPISIPLRRTISVKDVISVLGRETQMSRSILIHRLYANV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CL56</accession>
    <name>A0AAD5CL56_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Peptide-methionine (R)-S-oxide reductase</fullName>
            <ecNumber evidence="8">1.8.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_023955</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the reduction of methionine sulfoxide (MetSO) to methionine in proteins. Plays a protective role against oxidative stress by restoring activity to proteins that have been inactivated by methionine oxidation. MSRB family specifically reduces the MetSO R-enantiomer.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>L-methionyl-[protein] + [thioredoxin]-disulfide + H2O = L-methionyl-(R)-S-oxide-[protein] + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:24164"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="Rhea" id="RHEA-COMP:12313"/>
            <dbReference type="Rhea" id="RHEA-COMP:12314"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16044"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:45764"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.8.4.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the MsrB Met sulfoxide reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.8.4.12" evidence="8"/>
    <dbReference type="EMBL" id="JAMZMK010007624">
        <property type="protein sequence ID" value="KAI7743941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CL56"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033743">
        <property type="term" value="F:peptide-methionine (R)-S-oxide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030091">
        <property type="term" value="P:protein repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.150.20:FF:000001">
        <property type="entry name" value="Peptide methionine sulfoxide reductase MsrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.20">
        <property type="entry name" value="Peptide methionine sulfoxide reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028427">
        <property type="entry name" value="Met_Sox_Rdtase_MsrB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002579">
        <property type="entry name" value="Met_Sox_Rdtase_MsrB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011057">
        <property type="entry name" value="Mss4-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00357">
        <property type="entry name" value="peptide-methionine (R)-S-oxide reductase MsrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10173">
        <property type="entry name" value="METHIONINE SULFOXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10173:SF52">
        <property type="entry name" value="METHIONINE-R-SULFOXIDE REDUCTASE B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01641">
        <property type="entry name" value="SelR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51316">
        <property type="entry name" value="Mss4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51790">
        <property type="entry name" value="MSRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0249">Electron transport</keyword>
    <keyword evidence="2 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="3 8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="MsrB" evidence="10">
        <location>
            <begin position="77"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="46"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="46"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="68"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU365044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7743941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="199" mass="22314" checksum="47306B9AA82464BD" modified="2024-05-29" version="1">MASHSLTTSTHFRLSLNFSSKTHLNSALKSSNRVRFRAPTTRSYSLTRVMGSSSSSQKPDTTNQQETGNRDYKSVSDQEWKTKLTGEQFYVARQKGTERAFTGEYWNTKTPGTYHCICCDTPLFESTTKFDSGTGWPSYYEPIGTNVKSKLDLSIIFMPRQEVLCAVCDAHLGHVFDDGPPPTGKRYCINSASLKLNPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CLF8</accession>
    <name>A0AAD5CLF8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_030341</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007584">
        <property type="protein sequence ID" value="KAI7744091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CLF8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000051">
        <property type="entry name" value="Heterogeneous nuclear ribonucleoprotein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48033:SF4">
        <property type="entry name" value="OS08G0320100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48033">
        <property type="entry name" value="RNA-BINDING (RRM/RBD/RNP MOTIFS) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="15"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="105"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="180"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7744091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="359" mass="36826" checksum="47F9259C4E1E1369" modified="2024-05-29" version="1">MGSKRPDFGDGASPGKIFIGGLAKDTTIDTFVKYFGKYGDLTDSVIMKDRQTGRPRGFGFITYADPSVVDTVIAETHVINGKQVEIKRTIPKGSGESKPKDFKTKKIFVGGIPTSVTEDEFKGFFSKYGNVVEHEIIRDHATKRSRGFGFIVFDDDQVVDTIMVDGNMIDMNGTKVEIKKAEPKSKNPGPVSYGGGEPRGRGYGDSYGGYGDSYGGYGGDGGGYGPPPYRSLGGLSSRYGDYGGYGPSGGEFSSRYADYGGDYGGYRGGEPPLGGYSSRFSSFGSGGYGAGYGGSGVGGPYGRGGAGGGYGGYGGPGGAGGGYEPGGGSGYGGPGGPYGGRAGYGGGAGGGARYHPYGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CNG6</accession>
    <name>A0AAD5CNG6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">25S rRNA (uridine-N(3))-methyltransferase BMT5-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_027938</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007676">
        <property type="protein sequence ID" value="KAI7743809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CNG6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070042">
        <property type="term" value="F:rRNA (uridine-N3-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019446">
        <property type="entry name" value="BMT5-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11538:SF63">
        <property type="entry name" value="25S RRNA (URIDINE-N(3))-METHYLTRANSFERASE BMT5-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11538">
        <property type="entry name" value="PHENYLALANYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10354">
        <property type="entry name" value="BMT5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="25S rRNA (uridine-N(3))-methyltransferase BMT5-like" evidence="2">
        <location>
            <begin position="43"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7743809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="235" mass="26235" checksum="BF9836F37EE0BCAA" modified="2024-05-29" version="1">MSSSSSAALESDDGSSSSSSDNDQEQVKKDIWKGHYSSAHKILLVGEGDFSFSLCLARAFGSARNIVATTVDTHQELVKKYSNVVENVRELQDRGSVVLYGVDATAMSRHFFLTTQRFHRIVYNFPHVGFHFPEGNGCQIKLNKQLVKGFLRNAKTLLKKENGEIHVSHKVGEPYDSWDLVKKAEKIGLSLHKTSRFSKKMYPGYAPKRAHGAAADAAFKLGESVTYKFRLRECT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CQE0</accession>
    <name>A0AAD5CQE0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_024355</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007013">
        <property type="protein sequence ID" value="KAI7746281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CQE0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03213">
        <property type="entry name" value="ABCG_EPDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000932">
        <property type="entry name" value="ABC transporter G family member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052215">
        <property type="entry name" value="Plant_ABCG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48042">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48042:SF12">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="107"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="62"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="12"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="76"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7746281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="376" mass="41371" checksum="AB85C1F942320D1B" modified="2024-05-29" version="1" fragment="single">MEEIQSHSENYRSSSSSASSPASRVPSSNFFYLRKQGALRQPISFEDSPDWEENTDVEVRVEEGGDSINTATTPISPSLSKLNSGSLPSPPLPEGAVVARKTAGAAIVWKDLTVTIKGKRKYSDRVVKSSNGYALPGTMTVIMGPGKSGKSTLLRALAGRLDDSAKTYGEVFVNGAKSSLQYGSYGYVERKNTLIGSLTVREFLYYSALLQLPGFFYQKKSEVEDAIQAMSLGDYANKLIGGHCYMKGLPSGERRRVSIARELVMRPQVLFIDEPLYHLDSVSALLMMVTLKKLASTGCTLIFTIYQSSTEVFGLYDRICLLSNGNTLFFGETLACLQHFSNAGFPCPIMQSPSDHFLRAINTDFDRIITMCKTWQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CTQ7</accession>
    <name>A0AAD5CTQ7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein RADIALIS-like 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_012877</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006605">
        <property type="protein sequence ID" value="KAI7748088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CTQ7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048262">
        <property type="term" value="P:determination of dorsal/ventral asymmetry"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009908">
        <property type="term" value="P:flower development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000154">
        <property type="entry name" value="Transcription factor SRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044636">
        <property type="entry name" value="RADIALIS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43952">
        <property type="entry name" value="MYB FAMILY TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43952:SF68">
        <property type="entry name" value="PROTEIN RADIALIS-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23082">
        <property type="entry name" value="Myb_DNA-binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Myb-like" evidence="5">
        <location>
            <begin position="6"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="6">
        <location>
            <begin position="9"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7748088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="79" mass="8905" checksum="3A72AB243DD8DF91" modified="2024-05-29" version="1">MASSSMSSRESSSWTVKQNKAFEKALAVFDKDTPDRWHNVAKAVGGKTAEEVKQHYQVLVEDLKSIESGRVPYPNYRTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CTW9</accession>
    <name>A0AAD5CTW9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glycosyltransferase</fullName>
            <ecNumber evidence="5">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_010460</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="5"/>
    <dbReference type="EMBL" id="JAMZMK010006970">
        <property type="protein sequence ID" value="KAI7746466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CTW9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035251">
        <property type="term" value="F:UDP-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000020">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050481">
        <property type="entry name" value="UDP-glycosyltransf_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48048">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48048:SF30">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7746466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="425" mass="46718" checksum="E546F8E3060F4221" modified="2024-05-29" version="1">MPTIVLYPAPAMGHLISMVELGKFILKHHPNYSITVLTLTNSFNTGSTVSYVRHISATVPAISFHHLPDIALDLEAYPTSEAIIFDLIGRSVHNVDAALRSISPTALIIDLFCTSAMMAAASVNIPVYYFITSGACCLVEILYFPVLDRDYLGSFRDMNRLVYSPGMPPIPSSDMPAVPIKAITDGLCVEGHTPPLYCVGPVLSDGGDGSHECLKWLDSQPSESVVYLCFGSEGLFSCDQLKEIAKGLEMSGQRFLWVIRSPPTVKKGELFTPPELPDLDSLLPDRFLERTKDRGLVVKGWAPQVKVLSHDSVGGFVTHCGWNSVLESVRAGVPMVAWPQYAEQRMNKIVMVEEMKVALPMDESERGKVAAAEVGRRVRQLMESEEGKFVREVVKARKEDAARAINHTNGSSRVALGKLVESWTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CV38</accession>
    <name>A0AAD5CV38_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_024935</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006453">
        <property type="protein sequence ID" value="KAI7748848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CV38"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901259">
        <property type="term" value="P:chloroplast rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd21608">
        <property type="entry name" value="RRM2_NsCP33_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050502">
        <property type="entry name" value="Euk_RNA-bind_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048289">
        <property type="entry name" value="RRM2_NsCP33-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025:SF3">
        <property type="entry name" value="31 KDA RIBONUCLEOPROTEIN, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025">
        <property type="entry name" value="OS02G0815200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="3" id="KW-0934">Plastid</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="6 8" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="74"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7748848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="159" mass="17625" checksum="8BB671C46F7102E9" modified="2024-05-29" version="1" fragment="single">VFVGGLKVIYNRETDQSRGFGFVTMSSVEEAEKAIDMFHGYELGGRILTVNKAAAKGSRPVRVERTSLNSGPSYTMYVGNLPWDTDGERLEHIFREHGKVVNARIVYDRESGRSRGFGFVSMSSESEMNAAIETLDGQSLGGRAIRVNVAEERPRRGSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CW51</accession>
    <name>A0AAD5CW51_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NADP-dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_000106</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006501">
        <property type="protein sequence ID" value="KAI7748567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CW51"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044550">
        <property type="term" value="P:secondary metabolite biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19124">
        <property type="entry name" value="AKR_AKR4A_4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000014">
        <property type="entry name" value="NAD(P)-linked oxidoreductase superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044497">
        <property type="entry name" value="AKR4A/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11732">
        <property type="entry name" value="ALDO/KETO REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00798">
        <property type="entry name" value="ALDOKETO_REDUCTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00062">
        <property type="entry name" value="ALDOKETO_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00063">
        <property type="entry name" value="ALDOKETO_REDUCTASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="5">
        <location>
            <begin position="21"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="56"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Tyr" evidence="4">
        <location>
            <position position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7748567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="321" mass="36153" checksum="1501D4CE1C99944C" modified="2024-05-29" version="1">MVSIPETTISSSDGRRPIPLMGLGTATRTESGDQVKAAVIEAIKVGYRHFDTASLYGTEKPLGEAIQEALRLGLIKSRAELFITTKLWCDSTEGHLVVPALNKSLRELGLEYVDLYLIHWPLKLNQDDVKSPLTKDNIAAINLKDVWEGMEKCQDLGLTKSIGVSNFYPRLIDEILSFAKIPPVVNQVEMNPLWQQKNLNNFCKKNDILLTAYSPLGAFGTEWGHNRVMECDVLQDIAKSRGKTVAQISLRWMYEQGVSFVVKSFNTERMKQNLDIFDWSLTEDELIKISQIPQQRHVYLSGTMLKEPHDVLAEIDAGLSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CWV4</accession>
    <name>A0AAD5CWV4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">WAT1-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_012134</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the drug/metabolite transporter (DMT) superfamily. Plant drug/metabolite exporter (P-DME) (TC 2.A.7.4) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006277">
        <property type="protein sequence ID" value="KAI7749776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CWV4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030184">
        <property type="entry name" value="WAT1-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31218">
        <property type="entry name" value="WAT1-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="9"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="48"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="77"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="105"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="192"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="219"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="260"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="285"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="7">
        <location>
            <begin position="17"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="7">
        <location>
            <begin position="189"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7749776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="321" mass="35504" checksum="5C5989CE06B61D01" modified="2024-05-29" version="1">MSKEWFQRVYVCTKPFLLMIFLQTGYAVNGLVVKSALNKGLNHYTFAVYRNAIAALVFGPFAFFLEKKVRPQMTISVFLKIMLLGLLEPVMDLNLFFAGMKLTTATFAAAMCNIVPAITFVMAWMLRLEMVKIKSLHSQGKIIGTLITVGGAMIMTLIRGPAIQFPWTNHHTLHHQNSANIVSTQDQIKGSIMIAASCFSLASFFIVQALTLKSYPAELSLTALVCMIGTLEGSILTVMVERANPSIWSINWDNKLFATVYSGIMCSGCTYYICGVIMQERGPVFVTAFNPLGMVIIAVLGSFILAEKLNLGRKCTQNLHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CX78</accession>
    <name>A0AAD5CX78_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">3-hydroxy-3-methylglutaryl coenzyme A reductase</fullName>
            <shortName evidence="12">HMG-CoA reductase</shortName>
            <ecNumber evidence="12">1.1.1.34</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_029137</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>(R)-mevalonate + 2 NADP(+) + CoA = (3S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:15989"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:36464"/>
            <dbReference type="ChEBI" id="CHEBI:43074"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.34"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 3/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Endoplasmic reticulum membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the HMG-CoA reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.34" evidence="12"/>
    <dbReference type="EMBL" id="JAMZMK010006402">
        <property type="protein sequence ID" value="KAI7749122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CX78"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004420">
        <property type="term" value="F:hydroxymethylglutaryl-CoA reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015936">
        <property type="term" value="P:coenzyme A metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009753">
        <property type="term" value="P:response to jasmonic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016135">
        <property type="term" value="P:saponin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016126">
        <property type="term" value="P:sterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016104">
        <property type="term" value="P:triterpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00643">
        <property type="entry name" value="HMG-CoA_reductase_classI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3270.10:FF:000002">
        <property type="entry name" value="3-hydroxy-3-methylglutaryl coenzyme A reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.420:FF:000001">
        <property type="entry name" value="3-hydroxy-3-methylglutaryl coenzyme A reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.770.10:FF:000001">
        <property type="entry name" value="3-hydroxy-3-methylglutaryl coenzyme A reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.770.10">
        <property type="entry name" value="3-hydroxy-3-methylglutaryl-coenzyme A Reductase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3270.10">
        <property type="entry name" value="HMGR, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.420">
        <property type="entry name" value="Hydroxymethylglutaryl-CoA reductase, class I/II, NAD/NADP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002202">
        <property type="entry name" value="HMG_CoA_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023074">
        <property type="entry name" value="HMG_CoA_Rdtase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023076">
        <property type="entry name" value="HMG_CoA_Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004554">
        <property type="entry name" value="HMG_CoA_Rdtase_eu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023282">
        <property type="entry name" value="HMG_CoA_Rdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009023">
        <property type="entry name" value="HMG_CoA_Rdtase_NAD(P)-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009029">
        <property type="entry name" value="HMG_CoA_Rdtase_sub-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00533">
        <property type="entry name" value="HMG_CoA_R_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10572">
        <property type="entry name" value="3-HYDROXY-3-METHYLGLUTARYL-COENZYME A REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10572:SF56">
        <property type="entry name" value="3-HYDROXY-3-METHYLGLUTARYL-COENZYME A REDUCTASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00368">
        <property type="entry name" value="HMG-CoA_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00071">
        <property type="entry name" value="HMGCOARDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55035">
        <property type="entry name" value="NAD-binding domain of HMG-CoA reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56542">
        <property type="entry name" value="Substrate-binding domain of HMG-CoA reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00066">
        <property type="entry name" value="HMG_COA_REDUCTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00318">
        <property type="entry name" value="HMG_COA_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01192">
        <property type="entry name" value="HMG_COA_REDUCTASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50065">
        <property type="entry name" value="HMG_COA_REDUCTASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0521">NADP</keyword>
    <keyword evidence="8 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="83"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7749122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="579" mass="62359" checksum="8FBACCF895A3E4C4" modified="2024-05-29" version="1">MDARRQSSLKPHPSDQQLVFKPNNKKMVAGSNASNPLPTNSMAISNGIFFTVFFSVTYFLLIRWREKIRDSTPLHVVTISEMAAIMVFVASSIYLLGFFGISFVQTVPFSDDDEEEEKEEEILRKEDTRVTSCGAALDCESDVVVKHVINKDLVNLPKETTVTEEDAALIQEVVSGKIPSYSLESKLGNCQRAAFVRRMALERLTGKSLEGLPLEGFDYQSILGQCCEMPVGYVQIPVGIAGPMLLDGKEFWVPMATTEGCLVASTNRGCKAIYVSGGATSVLLKDGMTRAPVVRFGTAKRAAELKFFLEEPLNFDTLASAFNKSSRFGRLQRIQCAIAGKNLYIRFSCSTGDAMGMNMVSKGVQNVLDYLQLDFPDMDVLGISGNYCSDKKPAAVNWIEGRGKSVVCEAIIKEEVVKKVLKTTVASLVELNMLKNLTGSAMAGALGGFNAHASNIVSAVYIATGQDPAQNIESSHCITMMEAVNEGKDLHVSVTMPSIEVGTVGGGTQLSSQSACLNLLGVKGANKESAGMNARQLAKVVAASVLAGELSLMSAIAAGQLVKSHMKYNRSSKDIAAKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CXX9</accession>
    <name>A0AAD5CXX9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Knottins-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_000105</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006501">
        <property type="protein sequence ID" value="KAI7748566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CXX9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00107">
        <property type="entry name" value="Knot1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.10">
        <property type="entry name" value="Knottin, scorpion toxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008176">
        <property type="entry name" value="Defensin_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003614">
        <property type="entry name" value="Knottins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036574">
        <property type="entry name" value="Scorpion_toxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33147">
        <property type="entry name" value="DEFENSIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33147:SF129">
        <property type="entry name" value="DEFENSIN-LIKE PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00304">
        <property type="entry name" value="Gamma-thionin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00288">
        <property type="entry name" value="PUROTHIONIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00505">
        <property type="entry name" value="Knot1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57095">
        <property type="entry name" value="Scorpion toxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00940">
        <property type="entry name" value="GAMMA_THIONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042018118" description="Knottins-like domain-containing protein" evidence="5">
        <location>
            <begin position="21"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="Knottins-like" evidence="6">
        <location>
            <begin position="33"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7748566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="78" mass="8619" checksum="A49D9E46DB138075" modified="2024-05-29" version="1">MKSSMKLIATLLLVVMCLMANEMGGPIVVEARTCLSQSHKFKGTCLSDTNCGNVCKSEGFPRGKCRGFRRRCFCVKHC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CYG0</accession>
    <name>A0AAD5CYG0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_015600</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006289">
        <property type="protein sequence ID" value="KAI7749745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CYG0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14241:SF24">
        <property type="entry name" value="G DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14241">
        <property type="entry name" value="INTERFERON-INDUCED PROTEIN 44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7749745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="365" mass="41109" checksum="6B14AE6A28E2673A" modified="2024-05-29" version="1">MTNLHSSSPSPFCWWKSIPELSSHDASLKPPSSSSSSSMMKLLREMERLSLISEHGVDDLKHKLMIYKSGDFWVPIGGIKKEDMDIPPVITVLLVGLSGSGKSSLINLMYSVVGRSGFIPFSQTSNEMSNYSTMILEEHNVLRSTRNGFCVYDSRGLDCDRIEDCLKEVTRWMTDGVRHNEPCRFGDEDVSVGVHGSYDGFVRRKVNYVMVVVDLSDVHKAFFRGGDFKAVEAVKSLFRCPSLKTSNVDPILILTHGDMLQPMERLNCRIKICSYLGIPVTTGAYDIVCLTEQGILAEESDSITSFALTEAIYRSLLQSDQTHLPKKTYKDWTIDVLSRGMCSLAYFFDVLSRILQRWSDKYKTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CYN1</accession>
    <name>A0AAD5CYN1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_011141</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006280">
        <property type="protein sequence ID" value="KAI7749767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CYN1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008479">
        <property type="entry name" value="DUF760"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33598">
        <property type="entry name" value="OS02G0833400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33598:SF4">
        <property type="entry name" value="OS02G0833400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05542">
        <property type="entry name" value="DUF760"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="53"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="90"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="270"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="93"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7749767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="399" mass="44911" checksum="0F0326AD7A7646F2" modified="2024-05-29" version="1">MSYFLTLKTPFLSSHSSSSSSVLASCKNNHLLRLSSVYFSSKPSFNRGRLRLRAESQPQDSSQSSNSSSDSQQPNGSVLCYVIRLKLRPRAESQPQDSSNSGNSVIDGGGREGVEDAMKTLLKQHNKYICVCVELWRELVKTSRRDMLLEYVKNVQPEFMELFVKRAPQQVVDAMRQTVTNMMGTLPPQFFAVTVTTVAENLAQLMYSVLMTGYMFRNAQYRLELQQSLEQVALPDTSQEEKDAPVYAPGTQKKVTGEVIRWNNVSGPEKIDAIKYIELLEAEVEELNRQVERISANGSNELLDYLKTLEPQNLKDLTSSAGEDVVVAMNTFIKRLLAVSDPTQMKTSVTETSAPELAKLLYWLMVVGYSIRNIEVRYDMERVLGSPPKRQELPPAENI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CYR2</accession>
    <name>A0AAD5CYR2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tyrosine-protein phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_014688</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006160">
        <property type="protein sequence ID" value="KAI7750362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CYR2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043036">
        <property type="term" value="C:starch grain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019203">
        <property type="term" value="F:carbohydrate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004721">
        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005983">
        <property type="term" value="P:starch catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14526">
        <property type="entry name" value="DSP_laforin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02859">
        <property type="entry name" value="E_set_AMPKbeta_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032640">
        <property type="entry name" value="AMPK1_CBM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045204">
        <property type="entry name" value="DSP_laforin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47661">
        <property type="entry name" value="PHOSPHOGLUCAN PHOSPHATASE LSF1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47661:SF2">
        <property type="entry name" value="PHOSPHOGLUCAN PHOSPHATASE LSF1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16561">
        <property type="entry name" value="AMPK1_CBM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="4">
        <location>
            <begin position="267"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7750362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="566" mass="63453" checksum="6E090E29DFBD5153" modified="2024-05-29" version="1">MSLLHASNYGVFCQSQCSRSFLLTHSSVNASMCSLFWGKEVCMINEDSTDGYFRRRNGLEGRRIKVLAMSASTNPRFKMNLNEYMVTLEKPLGIRFALSLDGKILVHSMKKGGNAEKSRIIMVGDTLKKASASSGGSLIEIKDFSDADDLDIQFNRGRVPIATWNKSILTSSLQASSEGNGNPGFIVFTPRFSTPKGWNLLINQNGQPRGRIQKNIPDEPFTQLVTIFSDQESGDDWSHGSFPLEEYLKALDRSRGELYYDHSLGMRYSKITDQMYVGSCIQTEEDIETLANVAGVTAVLNFQGGNEAENWEIDSKSINESCQQNNILMINYPIRERDSFDLRSKLPFCVGLLLRLLKKNHCVYVTCTTGFDRSPTCVIAYLHWMTDTSLHAAYNYVSGLHTCRPDRPAIAWATWDLIAMVENGKHDGPATHSVTFVWNGHEGENVELIGDFSNWKEPIKATHKGGPRYEAEVRLAQGKYYYKFIVNGDWRHSTSSPTERDDRGNLNNILEVGGIANVRPSIPQPKKDANIAKVIERPLTENERFTLAKAARCIAFSVCPIRLAPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CYR6</accession>
    <name>A0AAD5CYR6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_011766</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006272">
        <property type="protein sequence ID" value="KAI7749812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CYR6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090575">
        <property type="term" value="C:RNA polymerase II transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015660">
        <property type="entry name" value="MASH1/Ascl1a-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13935">
        <property type="entry name" value="ACHAETE-SCUTE TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13935:SF63">
        <property type="entry name" value="BHLH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="5">
        <location>
            <begin position="13"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7749812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="224" mass="25536" checksum="A74B4447F4E2493B" modified="2024-05-29" version="1">MEGSIKCSSSRTLQKLERKVIEKNRRNQMKFLYSRLYSLVPPHFISKGCGASDRVDRTIEYIQSLKDNVEVNKKKKEKLLSQKRLRDNTKINSNMSYESLDIQIHEIGYQLDAVLVTGLNNYSSFCDVVQLLGRYSDEVTLANFSSNGHSTFHIRQKKIEADDVCKRLKNLGDGYSSSIKELPLNDASLSFCNELVEPDLSIWDFDFQSSVWGCDLGVMLQTSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D0Y2</accession>
    <name>A0AAD5D0Y2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cas1p 10 TM acyl transferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_013011</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PC-esterase family. CASD1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006093">
        <property type="protein sequence ID" value="KAI7750709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D0Y2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016407">
        <property type="term" value="F:acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009834">
        <property type="term" value="P:plant-type secondary cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045492">
        <property type="term" value="P:xylan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010411">
        <property type="term" value="P:xyloglucan metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012419">
        <property type="entry name" value="Cas1_AcylTrans_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13533">
        <property type="entry name" value="N-ACETYLNEURAMINATE 9-O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13533:SF48">
        <property type="entry name" value="PROTEIN REDUCED WALL ACETYLATION 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07779">
        <property type="entry name" value="Cas1_AcylT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Cas1p 10 TM acyl transferase" evidence="8">
        <location>
            <begin position="93"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7750709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="177" mass="20634" checksum="A3AA265FC94206A2" modified="2024-05-29" version="1" fragment="single">MVIRGGQQPHLWVTVRVPNKGCFLQKPYLGTCRDSDINLVELGNDTVKEDDRVVLLEGGTLRSASPISHSSTIASPVVKLITMDESFFLENRLTLRAILEFGLTMFYFYLCDRTNFFAHSTKYQQLPLSRYIMINHLPRKAIMYLNRHQTEEWKGWMLVLFLVYHYFAATEIYNAIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D2M5</accession>
    <name>A0AAD5D2M5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Arogenate dehydratase</fullName>
            <ecNumber evidence="3 8">4.2.1.91</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_018309</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Converts the prephenate produced from the shikimate-chorismate pathway into phenylalanine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-arogenate + H(+) = L-phenylalanine + CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:12536"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:58180"/>
            <dbReference type="EC" id="4.2.1.91"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Amino-acid biosynthesis; L-phenylalanine biosynthesis; L-phenylalanine from L-arogenate: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Plastid</location>
            <location evidence="1 8">Chloroplast stroma</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.91" evidence="3 8"/>
    <dbReference type="EMBL" id="JAMZMK010005606">
        <property type="protein sequence ID" value="KAI7752778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D2M5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009570">
        <property type="term" value="C:chloroplast stroma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047769">
        <property type="term" value="F:arogenate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004664">
        <property type="term" value="F:prephenate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009094">
        <property type="term" value="P:L-phenylalanine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04905">
        <property type="entry name" value="ACT_CM-PDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001086">
        <property type="entry name" value="Preph_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018528">
        <property type="entry name" value="Preph_deHydtase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21022:SF42">
        <property type="entry name" value="AROGENATE DEHYDRATASE_PREPHENATE DEHYDRATASE 2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21022">
        <property type="entry name" value="PREPHENATE DEHYDRATASE P PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00800">
        <property type="entry name" value="PDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00857">
        <property type="entry name" value="PREPHENATE_DEHYDR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00858">
        <property type="entry name" value="PREPHENATE_DEHYDR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51171">
        <property type="entry name" value="PREPHENATE_DEHYDR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5 8" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="7 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="6 8" id="KW-0584">Phenylalanine biosynthesis</keyword>
    <keyword evidence="8" id="KW-0934">Plastid</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Prephenate dehydratase" evidence="9">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="ACT" evidence="10">
        <location>
            <begin position="79"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7752778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="175" mass="19586" checksum="51FD8FD143F46E57" modified="2024-05-29" version="1">MIDYSLLFANVLYCHPSWQHVALHKLDDTGAVASAAAAKIYGLNLLQEDIQDYSDNVTRFLMLAREPIIPRTDRPFKTSIVFSLDEGPGMLFKALAVFAMRQINLTKIESRPLQRQALPAHNESTNGSPGSFPYLFYVDFQASMADQRAQNALSHLKEFATFLSVLGSYPEDISI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D3Z9</accession>
    <name>A0AAD5D3Z9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_033240</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005647">
        <property type="protein sequence ID" value="KAI7752655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D3Z9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47426">
        <property type="entry name" value="ACYL-COA N-ACYLTRANSFERASES (NAT) SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47426:SF4">
        <property type="entry name" value="N-ACETYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="228"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7752655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="306" mass="35507" checksum="F0727273F9DFE3C9" modified="2024-05-29" version="1" fragment="single">DQLVKRDVFCYELSHGSLIPHFKIRRPRLVFYKRIKKQDSLLIVDLFRDNFPISYTRCRTLETEWDNTQSIRHLIVPSNDDSKQPELSFNRLQLSDEEYTDMQCSFGRYIAREAILNEEYWMAAWLRAEAHWEASAYKRHAEIFKIKYADQEFKALKKRCSGQDGNLLQCFCLVAVKKEVKNMKRTVLNSIVGTMDLSIRQYLQGETYPGEIKKQSCVLASKQPLDAHKYAYISNVVVAKYARRRRIAINMLGLAAHIAASVGMKKLYVHVMADNKPGQELYKKGGFEFVTTNASNNGQLLMLMEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D482</accession>
    <name>A0AAD5D482_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_021757</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase A1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005602">
        <property type="protein sequence ID" value="KAI7752805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D482"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032799">
        <property type="entry name" value="TAXi_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032861">
        <property type="entry name" value="TAXi_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13683">
        <property type="entry name" value="ASPARTYL PROTEASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13683:SF875">
        <property type="entry name" value="EUKARYOTIC ASPARTYL PROTEASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14541">
        <property type="entry name" value="TAXi_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14543">
        <property type="entry name" value="TAXi_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042021879" description="Peptidase A1 domain-containing protein" evidence="2">
        <location>
            <begin position="21"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="3">
        <location>
            <begin position="80"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7752805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="311" mass="33309" checksum="9311CC6085684A95" modified="2024-05-29" version="1">MKLLIVLLVAAAAAVAVVNGGFPAKVLSLERVFPMKETVEIEVVRRRDSVRHKRMLQRFSGGIVDFNVVGTSDPYYGGLYFTKVKLGSPAKEFNVQIDTGSDVLWVTCSSCSGCPRSSGLQIDLNFFDAGSSSTASTVSCSDSICSSLVQTSDASCSAGNQCGYKFQYGDGSGTSGHYVTDLLHFDTIVGPSLIANSSASITFGCSTYQTGLRSFYLINSISGIFPDVSLNFAGGAAMILKPENYLVHGDPVNLCIYNLSEGGTPWCIGVQKAQNGVSILGDLVLKDKIFVYDLSKKRIGWTDYDCKHKVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D4C0</accession>
    <name>A0AAD5D4C0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">glucose-6-phosphate 1-epimerase</fullName>
            <ecNumber evidence="3 5">5.1.3.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_016996</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>alpha-D-glucose 6-phosphate = beta-D-glucose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:16249"/>
            <dbReference type="ChEBI" id="CHEBI:58225"/>
            <dbReference type="ChEBI" id="CHEBI:58247"/>
            <dbReference type="EC" id="5.1.3.15"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the glucose-6-phosphate 1-epimerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.15" evidence="3 5"/>
    <dbReference type="EMBL" id="JAMZMK010005657">
        <property type="protein sequence ID" value="KAI7752584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D4C0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047938">
        <property type="term" value="F:glucose-6-phosphate 1-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09020">
        <property type="entry name" value="D-hex-6-P-epi_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008183">
        <property type="entry name" value="Aldose_1/G6P_1-epimerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025532">
        <property type="entry name" value="G6P_1-epimerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014718">
        <property type="entry name" value="GH-type_carb-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11122">
        <property type="entry name" value="APOSPORY-ASSOCIATED PROTEIN C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11122:SF10">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE 1-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01263">
        <property type="entry name" value="Aldose_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016020">
        <property type="entry name" value="PHexose_mutarotase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="6">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR016020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR016020-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7752584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="310" mass="35104" checksum="751ECEADDBECB3BD" modified="2024-05-29" version="1">MGHSAAVWDTRMALETTKDWNGIEQIVLRNPLGASAKVSLLGGQVTSWRNERGEELLFMSSKGILKSPKAMRGGIPICFPQFSNCDSLEQHGFARNKIWVVDDDAPPLLSNDSQWKSFVDLLLKPSEEDLKFWPHSFEFRLRVSLGMDGNLTLISRVRNVNGKPFSFSFAYHSYLSVSDISEVRIEGLETMDYFDNLCKRERFTEQGDAITFESEIDRVYLSSPSCIAVLDHERKRTYVLKKEGLPDVVVWNPWEKKSKAMSDFGDDEYKQMLCVDGAAIEKPITLKPGEEWTGRLEIAVVPSSFCSEGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D4D0</accession>
    <name>A0AAD5D4D0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_008816</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005324">
        <property type="protein sequence ID" value="KAI7753693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D4D0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000554">
        <property type="entry name" value="protein indeterminate-domain 12-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000131">
        <property type="entry name" value="protein indeterminate-domain 5, chloroplastic-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055187">
        <property type="entry name" value="C2CH-3rd_BIRD-IDD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055185">
        <property type="entry name" value="C2CH-4th_BIRD-IDD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055186">
        <property type="entry name" value="C2H2-2nd_BIRD-IDD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031140">
        <property type="entry name" value="IDD1-16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10593:SF136">
        <property type="entry name" value="PROTEIN INDETERMINATE-DOMAIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10593">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE RIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22995">
        <property type="entry name" value="C2CH-3rd_BIRD-IDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22992">
        <property type="entry name" value="C2CH-4th_BIRD-IDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22996">
        <property type="entry name" value="C2H2-2nd_BIRD-IDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="96"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7753693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="456" mass="50087" checksum="A1F19A579860D5ED" modified="2024-05-29" version="1">MMFSPSVYSSSNKTSTSLSDQDAATSIVSPVITSKIHHEFNPSITLSSISSHIQEHDHVYPSPQKIKKKRNLPGNPDPDAEVIALSPKTLMATNRFVCEICNKGFQRDQNLQLHRRGHNLPWKLKQRNNKDEIKKRAYVCPEPSCVHHHPSRALGDLTGIKKHFCRKHGEKKWKCDKCSKIYAVQSDWKAHSKTCGTREYRCDCGTLFSRKDSFITHRAFCDALAEETARLSAAAAAASSISNTHHPSIINNVTNTPFNIHLTSQNSPSLFPFNHNSPNPNWIPTHDPNPTRIKPESIHSHDPTHHLLPANSLPFYPEPDPTRIISLPPVTFHGVNVSTGGYQLPVAAHMSATALLQKAATVGHVTGHVGSRMGGLDMGELGGHVTQVGMTSGNMATWQKTDRTRDFLGLTGDQEVDHHHGEANVSVRSMLTYAGGIEIPADGGFGTSGLDAWGNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D5V1</accession>
    <name>A0AAD5D5V1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">FACT complex subunit SSRP1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">M8C21_006408</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">AA19_3_7</strain>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Component of the FACT complex, a stable heterodimer of SPT16 and SSRP1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 12">Belongs to the SSRP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005651">
        <property type="protein sequence ID" value="KAI7752631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D5V1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035101">
        <property type="term" value="C:FACT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.30.10:FF:000016">
        <property type="entry name" value="FACT complex subunit SSRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.150:FF:000001">
        <property type="entry name" value="Fact complex subunit ssrp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000298">
        <property type="entry name" value="FACT complex subunit SSRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050454">
        <property type="entry name" value="RTT106/SSRP1_HistChap/FACT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048993">
        <property type="entry name" value="SSRP1-like_PH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000969">
        <property type="entry name" value="SSRP1/POB3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035417">
        <property type="entry name" value="SSRP1/POB3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45849">
        <property type="entry name" value="FACT COMPLEX SUBUNIT SSRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45849:SF6">
        <property type="entry name" value="FACT COMPLEX SUBUNIT SSRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21103">
        <property type="entry name" value="PH1_SSRP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17292">
        <property type="entry name" value="POB3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00887">
        <property type="entry name" value="SSRCOGNITION"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 12" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5 12" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9 12" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4 12" id="KW-0235">DNA replication</keyword>
    <keyword evidence="7 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10 11 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 12" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HMG box" evidence="14">
        <location>
            <begin position="249"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="11">
        <location>
            <begin position="249"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="176"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="291"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="183"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="200"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="291"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="320"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU364013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAI7752631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="330" mass="36668" checksum="4A0CBB4EA75E5F62" modified="2024-05-29" version="1">MADGHSFNNISLGGRGGTNPGQLKVHSGGVLWRKQGGGKAVEVDRSDILGLTWMKVPRSNQLAVKTKDGLKYKFTGFRDQNLLPMLHDHGRKLGRIWCAVASNTSMGRYNVELHLSFLRLQGQANDFKIQFSSVVRVFVLPKSNQPHTFVVVTLDPPIRKGQTMYPHIVLQDEDFVADKDDGGSPTDDSGGDDSNGSLSEGDKEKPVKKEPKKEPSTSKAVSNKKRTKSSEDDVSKKRKPKKKKDPNAPKRAMSGFMFFSQLERENVKKDIPGIAFQEIGRVLGERWNKLSAEEKEPYEAKAQVDKKRYQNEISGYKPKPSNSGNASDSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D5X6</accession>
    <name>A0AAD5D5X6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Bet v I/Major latex protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_026684</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BetVI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010002287">
        <property type="protein sequence ID" value="KAI7754898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D5X6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010427">
        <property type="term" value="F:abscisic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004864">
        <property type="term" value="F:protein phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009738">
        <property type="term" value="P:abscisic acid-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07816">
        <property type="entry name" value="Bet_v1-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.530.20:FF:000007">
        <property type="entry name" value="Major pollen allergen Bet v 1-A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000916">
        <property type="entry name" value="Bet_v_I/MLP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024949">
        <property type="entry name" value="Bet_v_I_allergen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050279">
        <property type="entry name" value="Plant_def-hormone_signal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31213:SF70">
        <property type="entry name" value="MAJOR ALLERGEN PRU AR 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31213">
        <property type="entry name" value="OS08G0374000 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00407">
        <property type="entry name" value="Bet_v_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00634">
        <property type="entry name" value="BETALLERGEN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bet v I/Major latex protein" evidence="2">
        <location>
            <begin position="1"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="Bet v I/Major latex protein" evidence="2">
        <location>
            <begin position="172"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7754898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="301" mass="33225" checksum="706BF130148491B5" modified="2024-05-29" version="1">MGVLTYTDEHTSPVIPTRIFKASIVDSHNLMPKLLPDAIKSITLIKGDGGVGSIKQINFAGGFAKHQIDEIDEKSLTYKYTLIEGNGISDKIEKVSYDIKFESSPDGGSISKMTTTIYTRGDFEIKEDELKAGKEKVLGLYKVVEAYLINNPDAYSVIFIQRFIKQAAILDSHNLMPKLLPEAIKSIELIKGDGGAGSIKQINFAGGFAKHQIDEIDEKTLTYKYTLIEGNGISDKIEKVSYDIKFESSSEGGTVSNMTTTIYTRGDIEIKEEELKAGKEKVLGLYKVAEAYLINNPDAYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D6B1</accession>
    <name>A0AAD5D6B1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">R3H-associated N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_032638</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005703">
        <property type="protein sequence ID" value="KAI7752375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D6B1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.50">
        <property type="entry name" value="R3H-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025952">
        <property type="entry name" value="R3H-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036867">
        <property type="entry name" value="R3H_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039629">
        <property type="entry name" value="R3HDM4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32019">
        <property type="entry name" value="R3H DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32019:SF2">
        <property type="entry name" value="R3H DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13902">
        <property type="entry name" value="R3H-assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82708">
        <property type="entry name" value="R3H domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="R3H-associated N-terminal" evidence="1">
        <location>
            <begin position="72"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7752375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="237" mass="26990" checksum="9864E6AA3E51F7F6" modified="2024-05-29" version="1" fragment="single">TFYLPQSLNFQSMATPDVVLQGEHDHFPFDHPKGDGKSRGLALEKKIEYLESLNDRAYGPCFWKIRLTLGTRDEAQPSPFSMTNVGEWDKFRNIDMDKEAGAIDALKGSSSKKKSSVDVDKIAALTAWHRVDCRTREAFRRSFLPELVNGFEVNIRAFVSEATNEEVLVMYAQDPFHRLLLHGVCEFYNLVSTTESETKGTKVLKMTKIKKKKKAGNKELPTITLCEFLKMAKDGFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D869</accession>
    <name>A0AAD5D869_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_025972</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010001697">
        <property type="protein sequence ID" value="KAI7755102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D869"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015020">
        <property type="term" value="F:glucuronosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044610">
        <property type="entry name" value="GLCAT14A/B/C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003406">
        <property type="entry name" value="Glyco_trans_14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45719:SF4">
        <property type="entry name" value="CORE-2_I-BRANCHING BETA-1,6-N-ACETYLGLUCOSAMINYLTRANSFERASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45719">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02485">
        <property type="entry name" value="Branch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7755102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="405" mass="46507" checksum="74323EC8244CC3EF" modified="2024-05-29" version="1">MSLKLFLISFTLTSLLFTMLYISTYLTPSNITSLTQPSLTTLVIPKTINNTKPYPVSFAYLISASKGDTAKLKRTLQALYHPNNLYLIHLELDATEQEHKEVARFVRDNNVFQTVGNVLVVGKPNRVTYRGPTMLATTLHAMSMLLRIDGTWDWFINLSASDYPLVTQDDLISAFSVMPKHLNFIQHTSRLGWKLNKRGKPIMIDPGLYSSNKSDIWWVIKQRTLPTAFKLYTGSAWTILSRSFAEYCIIGWENLPRTLLLYYTNFVSSPEGYFQTVICNSKDYKNTTVNHDLHYISWDTPPKQHPRLLGPRDYRKMVLSSRPFARKFRHNNAVLDRIDRELLKRRPNHFTHGGWCSVGDGKHQKCSNLNNDGFGVLRPGAGARRLRTLLTKLTSNPNFSRRQCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D9D4</accession>
    <name>A0AAD5D9D4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_030307</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000236">
        <property type="protein sequence ID" value="KAI7756893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D9D4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030126">
        <property type="term" value="C:COPI vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050844">
        <property type="entry name" value="Coatomer_complex_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19876">
        <property type="entry name" value="COATOMER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19876:SF76">
        <property type="entry name" value="WD40_YVTN REPEAT-LIKE-CONTAINING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="28"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="72"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7756893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="149" mass="16590" checksum="AA2C8641851BBDC3" modified="2024-05-29" version="1" fragment="single">DANVFASVSLDHTIKFWDLGSMDLRSSINGHTEGLNSVDFFEDEGKLYILTGSDDHTAKVWDYESMTCVQTLEGHTHNVTSFQCVKLNASFIITGSEDQTIRIWNSKTYNQDHVFASELGRVWTIGFIEGSSKVILGCDEGLVVGQVTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DBF0</accession>
    <name>A0AAD5DBF0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DC1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_008199</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000111">
        <property type="protein sequence ID" value="KAI7757503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DBF0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004146">
        <property type="entry name" value="DC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053192">
        <property type="entry name" value="Vacuole_Formation_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32410">
        <property type="entry name" value="CYSTEINE/HISTIDINE-RICH C1 DOMAIN FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32410:SF216">
        <property type="entry name" value="PHORBOL-ESTER_DAG-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03107">
        <property type="entry name" value="C1_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="DC1" evidence="2">
        <location>
            <begin position="4"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="DC1" evidence="2">
        <location>
            <begin position="184"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="DC1" evidence="2">
        <location>
            <begin position="312"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7757503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="359" mass="42094" checksum="36BCB809C29E54DC" modified="2024-05-29" version="1" fragment="single">MDHPSHPHQLQRSFDRMTSRCKACGNENSGTFYHCTSCSWFKIHLDCALLPAKLLIQQSTNESFRHSHLSNLVYSFPYIEQKAKFFPKCRVCDKNFPDTNIWHYRCNKCRYYVHIDCATSRKEAFMCCILMPAGQGKTHINFKVEDHPNLIRYPFPDESVNLVMHHFINKGEKTIKEKIVGEMFSHRHPLILVDTLLNGSISLHDPMKKVELLCDGCVRPITDVPFYKCCQQDCGFVLHERCTRLPSEIQHHPCHHPEHKLVLCQRSLKICWVCFGVRFVSYIAMVSHMDARNVNIIMWISIVGSYLMIRHKYDNHPLSLRYYPAENHSEEYFCEICEVELNPERWFYHCSGCALSMLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DCL7</accession>
    <name>A0AAD5DCL7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Brix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_017133</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the BRX1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000136">
        <property type="protein sequence ID" value="KAI7757349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DCL7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007109">
        <property type="entry name" value="Brix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026532">
        <property type="entry name" value="BRX1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13634">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN BRIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13634:SF0">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN BRX1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04427">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00879">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50833">
        <property type="entry name" value="BRIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Brix" evidence="6">
        <location>
            <begin position="62"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="8"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7757349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="296" mass="33956" checksum="AC3F96B5D38EAD3C" modified="2024-05-29" version="1">MGKKRKHSEAEAPAVKKEETAPERPKRTLLGWKEDNPQQQQSITGFRNREKVLVTCSRRINYSKVVELMVVNVFRYRHLMLNLVSVLPHCKKDNKVESKATKGATLNELVELKSCSSCLFFECRKHKDLYLWMSKCPNGPSVKFLVNAVHTMEELKLTGNHLKGSRPLLTFSSNFDKDPHWKLLKEMITQNMASELTISCPHTGADKIDRGGLEKMTLIEVGPRFCLNPIKIFGGSFGGPTLYENPFYVSPNQIRSLEKRQKAGKYAKKVKAKTRRKMHEMTNPLEADEFADMWKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DD30</accession>
    <name>A0AAD5DD30_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_007166</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000971">
        <property type="protein sequence ID" value="KAI7755630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DD30"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46225">
        <property type="entry name" value="C3H4 TYPE ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46225:SF1">
        <property type="entry name" value="RING_U-BOX SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="85"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="116"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7755630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="166" mass="18975" checksum="62787C4C296B4DAA" modified="2024-05-29" version="1" fragment="single">SLCNSAVAVSFSLISTAREEGTTEDVGPRNRAMTRPTAQVPSTFLVRMAIRISRARWYSFARRVFHYQNAARSDIGSNPFNTGSWMFMEFLGLVFQIVMITYTLSVSKEEKPVWPMRTWVSGYGAGCVLNLGLLFWRYRELAIDEQISNIYGAILCHMQSTRRRKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DDJ0</accession>
    <name>A0AAD5DDJ0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_015223</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000108">
        <property type="protein sequence ID" value="KAI7757519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DDJ0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7757519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="117" mass="13183" checksum="ED3EA3D0CD6F9644" modified="2024-05-29" version="1" fragment="single">MGMVVSVVVAEDQNIVAFSQGKLGSYLQDFSLPAYKISACLLFTSAILLFQFSLQQYWIQSACAGGLRHWLGYSLVVYLAISILWRYMEKSGLKETANSFHCEAKLYMLHVMGKPME</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DDN5</accession>
    <name>A0AAD5DDN5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Endoglucanase</fullName>
            <ecNumber evidence="10">3.2.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_016485</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>Endohydrolysis of (1-&gt;4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 8 10">Belongs to the glycosyl hydrolase 9 (cellulase E) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.4" evidence="10"/>
    <dbReference type="EMBL" id="JAMZMK010000097">
        <property type="protein sequence ID" value="KAI7757594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DDN5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008810">
        <property type="term" value="F:cellulase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001701">
        <property type="entry name" value="Glyco_hydro_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033126">
        <property type="entry name" value="Glyco_hydro_9_Asp/Glu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018221">
        <property type="entry name" value="Glyco_hydro_9_His_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22298">
        <property type="entry name" value="ENDO-1,4-BETA-GLUCANASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00759">
        <property type="entry name" value="Glyco_hydro_9"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00592">
        <property type="entry name" value="GH9_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00698">
        <property type="entry name" value="GH9_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8 10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4 10" id="KW-0136">Cellulose degradation</keyword>
    <keyword evidence="6 8 10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3 8 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 8 10" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycoside hydrolase family 9" evidence="11">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 9" evidence="11">
        <location>
            <begin position="67"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="341"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="389"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="398"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7757594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="447" mass="49331" checksum="7A6F58F7C65D91C8" modified="2024-05-29" version="1" fragment="single">GKLPKNNGIKWRGNSGLQDGADATDVKGGLVGGYYDAGDNTKFNFPMSFAMTMLSWSVGGSQNGSTTNDDHTCWQRPEDMDYKRPTQMITAGADLAGEMAAALAAASIVFKDDAEYSKKLVKGASTVWTFARDGGKRSPYSRGNLFIAPYYNSTGYYDEYLWGSAWMYYATGNSSYLWLATQEGLPRHARALSNNPSQRVLSWDNKSPAAMLLLTRMRVFLNPGYPYEDLLRSYHNATALTMCSFLQEYKTFNWTQGGLIELSQGRPQNLQYVVNAVFLASLYADYLETANLPGWYCGVTFFRTNRLREFATSQMDYILGNNPMKMSYVVGHGKKFPTHVHHRGASIPTNNIKYSCKGGFQWLSSGKPNPHNITGAMVGGPDRFDKYKDVRSNYSYTEPTLAGNAGLVAALVSLTTTAGDGVDKNSIFSAIPPLFPTAPPPPPPWKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DEG5</accession>
    <name>A0AAD5DEG5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein groES</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_025114</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Seems to function only as a co-chaperone, along with cpn60, and in certain cases is essential for the discharge of biologically active proteins from cpn60.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Forms stable complexes with CPN60 in the presence of ATP.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the GroES chaperonin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000044">
        <property type="protein sequence ID" value="KAI7757897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DEG5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00320">
        <property type="entry name" value="cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.33.40:FF:000002">
        <property type="entry name" value="10 kDa chaperonin, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.33.40">
        <property type="entry name" value="GroES chaperonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020818">
        <property type="entry name" value="Chaperonin_GroES"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037124">
        <property type="entry name" value="Chaperonin_GroES_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10772">
        <property type="entry name" value="10 KDA HEAT SHOCK PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10772:SF37">
        <property type="entry name" value="GROES-LIKE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00166">
        <property type="entry name" value="Cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00297">
        <property type="entry name" value="CHAPERONIN10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00883">
        <property type="entry name" value="Cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00053355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00062160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00083733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7757897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="143" mass="15781" checksum="7BA6650B5044BED8" modified="2024-05-29" version="1">MCLRRTKRSEIDSVPYSAMAKRLIPCLNRVLVEKIVPPSKTTAGILLPEKSSKLNSGKVIAVGPGTRDKSGNNIPVAVKEGDTVLLPEYGGTEVKLGEKEYVFLFCIICSGMRTFWELCMTSYSQTEIFSMSGCSNVMFEMLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-06-18" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DI86</accession>
    <name>A0AAD5DI86_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Scaffold protein Nfu/NifU N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_017366</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Molecular scaffold for [Fe-S] cluster assembly of mitochondrial iron-sulfur proteins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NifU family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000032">
        <property type="protein sequence ID" value="KAI7758060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.130:FF:000001">
        <property type="entry name" value="NFU1 iron-sulfur cluster scaffold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.70:FF:000001">
        <property type="entry name" value="NifU-like protein 4, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.130">
        <property type="entry name" value="Fe-S cluster assembly (FSCA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.70">
        <property type="entry name" value="Scaffold protein Nfu/NifU, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034904">
        <property type="entry name" value="FSCA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014824">
        <property type="entry name" value="Nfu/NifU_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036498">
        <property type="entry name" value="Nfu/NifU_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035433">
        <property type="entry name" value="NFU1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001075">
        <property type="entry name" value="NIF_FeS_clus_asmbl_NifU_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11178">
        <property type="entry name" value="IRON-SULFUR CLUSTER SCAFFOLD PROTEIN NFU-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11178:SF1">
        <property type="entry name" value="NFU1 IRON-SULFUR CLUSTER SCAFFOLD HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08712">
        <property type="entry name" value="Nfu_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01106">
        <property type="entry name" value="NifU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036773">
        <property type="entry name" value="HIRIP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00932">
        <property type="entry name" value="Nfu_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117916">
        <property type="entry name" value="Fe-S cluster assembly (FSCA) domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110836">
        <property type="entry name" value="Hypothetical protein SAV1430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Scaffold protein Nfu/NifU N-terminal" evidence="3">
        <location>
            <begin position="3"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7758060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="198" mass="21430" checksum="411BBB1609A07AEC" modified="2024-05-29" version="1">MFIQTQSTPNPLSLMFYPGKPVMEVGSADXXXXNSPLAKALFGIDGITRVFFGSDFVTVTKSDDASWDFLKPEIFAAIMDFYSSGKPLFIDAATSASMDTAIHEDDSETVAMIKELLETRIRPAVQDDGGDIEYIGFDPESGVVKLKMQGACSGCPSSSVTLKSGIENMLMHYVPEVKAVEQEFDAEDENGGLASELD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G241</accession>
    <name>A0AAD5G241_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Oleosin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_025812</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May have a structural role to stabilize the lipid body during desiccation of the seed by preventing coalescence of the oil. Probably interacts with both lipid and phospholipid moieties of lipid bodies. May also provide recognition signals for specific lipase anchorage in lipolysis during seedling growth.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Lipid droplet</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the oleosin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012146">
        <property type="protein sequence ID" value="KAI7724886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G241"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012511">
        <property type="term" value="C:monolayer-surrounded lipid storage body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019915">
        <property type="term" value="P:lipid storage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050826">
        <property type="term" value="P:response to freezing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010344">
        <property type="term" value="P:seed oilbody biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000136">
        <property type="entry name" value="Oleosin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33203">
        <property type="entry name" value="OLEOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33203:SF44">
        <property type="entry name" value="OLEOSIN 20.3 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01277">
        <property type="entry name" value="Oleosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00811">
        <property type="entry name" value="OLEOSINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="46"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="81"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7724886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="178" mass="18944" checksum="666392F8DBA6FE0D" modified="2024-05-29" version="1">MATTTYDRHQLTTTQPHYRHDQQARLTHPQHQQGPSTGKILTMMALLPLTGILFGLAGITLVGTVIGLAIATPLFVIFSPVIVPAAIAIGLAVTGFLTSGTFGLTGLSSLSYLFSMVRRSTMSVPDQMGYVKGKLQDVGEYTGQKTKDLGYKIQHTAHEMGDQGQGGRKEGGKGGDKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G480</accession>
    <name>A0AAD5G480_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DYW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_012906</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. PCMP-H subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011254">
        <property type="protein sequence ID" value="KAI7728214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G480"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000344">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000325">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein At4g14820"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032867">
        <property type="entry name" value="DYW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046848">
        <property type="entry name" value="E_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF409">
        <property type="entry name" value="DYW DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14432">
        <property type="entry name" value="DYW_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20431">
        <property type="entry name" value="E_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="5"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="137"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="238"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="DYW" evidence="4">
        <location>
            <begin position="455"/>
            <end position="547"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7728214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="547" mass="61383" checksum="EFF57CC04FD368D2" modified="2024-05-29" version="1">MPKKNVMSCNILIGGFVQNGELGNARKVFDEMPERNIATWNGMITGLTDFECNEEALRVLYEMRVSGFYLDEFTLGSALRGCAGLRSLNAGRQIHAYAVKYGLEVNVVVGSSLAHMYIKSGSLAEGENVIKSMPVHSVAVYNTLITGRAQSGCNEEALNQYHIMKISGFKPDKITFVTVLSSCSQLATLGQGQQIHAEAIKSGATSSVSVLSSLISMYSRCGCFKDALKSFSETKIHDVVLWSSMIAAYGFHGMGKEAVELFNQMEKEELVPNDVTFLSLLYACSHCGLKDEGIKIFDLMTEKYKLAPQLEHYTCVVDLLGRSGCLEEAENVIRSMPVNVKADSIIWKTLLSACRKYRNADMAKRIAEEVIRVDPHDSASYVLLSNIQASAKQWHGVSDYRKAMRDRRVKKEPGVSWFEMKNQVHQFCMKDKSHPDSEKIKLYLNELTGEMKLLGYKPDYGSILHDMDLEEKEDDLGQHSEKLAIAFALMNTPDCVPIRIMKNLRVCNDCHVAIKYISTIKKREIIVRDTSRFHHFKEGQCSCGDYW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G4P4</accession>
    <name>A0AAD5G4P4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">M8C21_019066</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">AA19_3_7</strain>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the syntaxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011166">
        <property type="protein sequence ID" value="KAI7728695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G4P4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048278">
        <property type="term" value="P:vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15848">
        <property type="entry name" value="SNARE_syntaxin1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00179">
        <property type="entry name" value="SynN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000008">
        <property type="entry name" value="Syntaxin 132"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010989">
        <property type="entry name" value="SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045242">
        <property type="entry name" value="Syntaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006012">
        <property type="entry name" value="Syntaxin/epimorphin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006011">
        <property type="entry name" value="Syntaxin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957:SF307">
        <property type="entry name" value="PROTEIN SSO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05739">
        <property type="entry name" value="SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00804">
        <property type="entry name" value="Syntaxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00503">
        <property type="entry name" value="SynN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47661">
        <property type="entry name" value="t-snare proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00914">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="8 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="274"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="14">
        <location>
            <begin position="202"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="25"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="15">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAI7728695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="301" mass="34202" checksum="51AA62EA65478956" modified="2024-05-29" version="1" fragment="single">MNDLLSTFSSPQRSSHGGGDIETGKRSAEIELDEFFKKVQHIENEFEKLYRLLKKLQDSHEESKSVTKAAAMKAIKKRMEHDVDEVGMIARIIKAKIEELDKENLTNRQKPGCGQGTGVDRSRTGTTLALKKKFKDKVTEFQTLRENIHQEHRAVVERRVFTVTGTRADEETIDRLIETGDSEQIFQKAIQEQGRGQVVDTLAEIQERHDAVIEVERKLLDLQQIFQDLAILVDAQGEILNNIETHVSSAVDHVQSANKALKKTKKLQKNTRKWICFAIVVLLIVISVVLVVVVKPWQSTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G585</accession>
    <name>A0AAD5G585_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">WRKY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_025316</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011296">
        <property type="protein sequence ID" value="KAI7727918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G585"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.80:FF:000006">
        <property type="entry name" value="WRKY transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.80">
        <property type="entry name" value="WRKY domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003657">
        <property type="entry name" value="WRKY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036576">
        <property type="entry name" value="WRKY_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044810">
        <property type="entry name" value="WRKY_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31221:SF360">
        <property type="entry name" value="WRKY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31221">
        <property type="entry name" value="WRKY TRANSCRIPTION FACTOR PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03106">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00774">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118290">
        <property type="entry name" value="WRKY DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50811">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="WRKY" evidence="8">
        <location>
            <begin position="152"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="133"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="206"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="16"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="139"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="215"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="257"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7727918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="270" mass="30440" checksum="A1D697A10F0606C0" modified="2024-05-29" version="1" fragment="single">DLIASNNNNNNNNNNQDTNWGFFSSNNSGVESPKVPATPFSPSSFLSTPTGLNQNQFLDSPFFPFNSNIVPSPTTGSFSGLDFKDDERKFSSFSFQPQPRPVSSVSANKMEEQVKSQPQEWSLTKQTDYIPEINKPKSESTQYVSDQSQATRDQTRLEDGYNWRKYGQKQVKGSENPRSYYKCTYPNCPTKKKVERNLEGHITEIVYKGSHTHSKPQNARKSSSNNYIEAPTENSHFDSSASFGDDDFEPASSISKSGDDHENEPEAKRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G6B6</accession>
    <name>A0AAD5G6B6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_025430</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011095">
        <property type="protein sequence ID" value="KAI7728983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G6B6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11393">
        <property type="entry name" value="bHLH_AtbHLH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045239">
        <property type="entry name" value="bHLH95_bHLH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045843">
        <property type="entry name" value="IND-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16223:SF46">
        <property type="entry name" value="TRANSCRIPTION FACTOR BHLH123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16223">
        <property type="entry name" value="TRANSCRIPTION FACTOR BHLH83-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="7">
        <location>
            <begin position="291"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="206"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="260"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="219"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7728983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="410" mass="45152" checksum="2459AEEA57C90E50" modified="2024-05-29" version="1">MADSWWDSRTTRPCLNSVTSMNLFQETTDSTTTTATCSGGGGSGGLLESNPNLQMMELGLSSQPISQSLDWNQALYRGDQRNDDNQSGYQTLIQEDHGLSSNTSNFQENQWKLHKMYSGSPSEFKQVSVRNLRLDEHGQYSDENRLNPGFQSMDDHSYGSNSTLIQSLFGSDSNTNLQQDSSYDQNQGISYSYPSSYGGMTISGGSGGGEVYAPPPPQQEFSTNSSPSKVQANLATQLQFSNNARFWNASASNFFPLQMQSPSSTIEDKPKNPISEMMKKGNTKSSSSSTKRPRNENPPMPPLKVKMGDRITALQQLVSPFGKTDTASVLSEAIVYIKFLHEQVNVLSTPYMNNAAIMQQQKQISDNPSDGSRQDLRSRGLCLVPISSTFPVTNETPVDFWTPSFGGTFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G7D2</accession>
    <name>A0AAD5G7D2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Glucosamine inositolphosphorylceramide transferase 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">M8C21_019999</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">AA19_3_7</strain>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the glycosyltransferase 64 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010852">
        <property type="protein sequence ID" value="KAI7730048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G7D2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.115.10.20:FF:000004">
        <property type="entry name" value="Glucosamine inositolphosphorylceramide transferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000095">
        <property type="entry name" value="Glycosyltransferase family protein 64 protein C5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.115.10.20">
        <property type="entry name" value="Glycosyl hydrolase domain, family 43"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004263">
        <property type="entry name" value="Exostosin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056442">
        <property type="entry name" value="GINT1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023296">
        <property type="entry name" value="Glyco_hydro_beta-prop_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015338">
        <property type="entry name" value="GT64_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48261">
        <property type="entry name" value="ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48261:SF6">
        <property type="entry name" value="GLYCOSYLTRANSFERASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24793">
        <property type="entry name" value="GINT1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09258">
        <property type="entry name" value="Glyco_transf_64"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75005">
        <property type="entry name" value="Arabinanase/levansucrase/invertase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0464">Manganese</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="360"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="417"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="domain" description="Glucosamine inositolphosphorylceramide transferase 1 N-terminal" evidence="16">
        <location>
            <begin position="55"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase 64" evidence="15">
        <location>
            <begin position="456"/>
            <end position="695"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00069035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF09258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF24793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAI7730048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="704" mass="80638" checksum="B0BAD8543B7E8B0F" modified="2024-05-29" version="1">MGWGPTSDKTHGSRWRVHPLFVSFLLCISFGVFYTFYLFGNNPNLGYYGSNSLGCQEDNDGSWGIGVFYGDSPFSLKPIEDMNIWDNKSAAWPVANPVLTCASVTDSGFPSNFVLDPFLYAQDDILYMFFESKNPITRQGDIGVARSMDDGATWEHLGVVLDKESHLSHPYVFDYNGQIYMMPDESSKGDVRVYRATEFPLKWKLEKIILDHPLVDAFIIQHEGKYWMFGSYQSQVTCQHLEIWYSNSPFGPWTPHSKNPVSGPRNGGRPFLYNGNLYRLAHDERVRVFKIEVLSVHNYKEVEVDLGVEKPTKGKNAWNGARSHHLNVHQLRTGKWLAVADGDRTVYGDVTSRHMVGCGLIITAGSIIYIICWLLGFVKCLVTYNSLKRKNGANLVWERLKLRLRLNINPNTYKGKFIVSLLVMISVTIMCVGFWYIYGGNGAEKPYPVDGRFSQFTLLAMTYDARIWNLKMYVKHYSRCASVREIVVVWNKGKPPNLNDLDSTVPVRIRVEEQNSLNNRFKTDPLIKTKAILELDDDIMMNCDDLERGFKIWRENPERLVGFYPRLVTGPPPLQYRPEKHARKHNGYNMILTGAAFMDRELAFERYWGKEAESGRNTVDEVFNCEDVLMNFLYANATVGSVSVEYVKPAWAIDTSKFSGVAISGNTQAHYRVRSKCLEKFTRLYGGLSDRKVEFRRRKDGWDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G7Q3</accession>
    <name>A0AAD5G7Q3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_027418</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010010419">
        <property type="protein sequence ID" value="KAI7731604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G7Q3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd14016">
        <property type="entry name" value="STKc_CK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055900">
        <property type="entry name" value="DUF7477"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24289">
        <property type="entry name" value="DUF7477"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="134"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="22"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7731604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="699" mass="77713" checksum="9D04689E83353F51" modified="2024-05-29" version="1">MPDLRRGVRQAKKGNNINVEDNAAALLAPTPRRGTRRGKVQALKSPAASPVVPAYPYPMPRPAGRGRGARPTDHDKNPDIFGGGIGRPPLNLDVGVCNPLVGGRSAEKLAADAEDEGSTSPLPERVQVGNSPAYRLERKLGKGGFGQVYVGRRVIGGSGNTGPDAVEVALKLEHRNGKGCSYGPPYEWQVYSTLNGCYGVPMVHHKGRQGDYYILVMDKLGPSLWDVWNSSNQILSEEMVACIAVEALSILEQLHLRGFVHGDVKPENFLLGQPGTPNEKKLYLVDLGLASKWRDATSGEHVQYDQKPDVFRGTVRYASVHAHLGRTGSRRDDLESLAYTLIFLLRGKLPWQGFIGENKGFLVCRKKMATSPEMLCYLCPSPFKQFLETVNNMKFDEEPNYLKLIGLFENCLVSTASLRPIRIDGALKVGQKRGRLLIDLEDDAQPRKKTRLGTPATQWISIYTSRAPMKQRYYYNVSDARLHQHVEKGKNDGLSISCVASSSNLWAIVMDAGTGFTSQVYELSPVFLHKEWIMEQWEKNYYITSLAGATNGSALVVMSKGTSYTQQSYKVSDVFPFKWINKKWKEGFLVTSMTTSGSRWGIVMSRDATYSNQVVELDFLYPSEGIHRRWENGYRITAAAATEDQAAFILSTSKKRSQDVTQETLRTSTFPSTHVKHPSVTEELYLDILKNLSELLPAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G7V4</accession>
    <name>A0AAD5G7V4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_023040</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010682">
        <property type="protein sequence ID" value="KAI7730676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G7V4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7730676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="81" mass="9314" checksum="4BD8FE1FDA36FD4A" modified="2024-05-29" version="1">MQSILNNGLPLCLAGVTCYHQLTRTKRIADHDRRREEFRRAQPRIRETEGVIRSLDLMTLNHDDDVDQLSIRHTTCAAKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G919</accession>
    <name>A0AAD5G919_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Fungal lipase-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_004240</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010102">
        <property type="protein sequence ID" value="KAI7732954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G919"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008970">
        <property type="term" value="F:phospholipase A1 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00519">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002921">
        <property type="entry name" value="Fungal_lipase-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043367">
        <property type="entry name" value="PLIP1/2/3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46483:SF1">
        <property type="entry name" value="PHOSPHOLIPASE A1 PLIP1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46483">
        <property type="entry name" value="PHOSPHOLIPASE A1 PLIP2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01764">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fungal lipase-type" evidence="3">
        <location>
            <begin position="376"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="8"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="152"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7732954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="682" mass="76894" checksum="A8CE97A793AE0E11" modified="2024-05-29" version="1">MACSIISISSQSTTPKAESSVGGKKPNILNRSNSSQNLRQQAGIRRAHSDNNLCYSANHVQASMNQPKLKNSRSVGIFNLNLSSSLIPNSLKTLFIDPDTNTDTDMYLDTDTEQRDVPADVNDVEMDKEEKKRANWIERLMEIRSQWVQKQNNKLDGENEEEKGCDEDGKGEGCEVCYSDDEDEVIINQETFSGMLKHVSWSDTKHFSQLAFLCNMAYVIPEIEEDDLRRYYDLTFVTSSLEKKVLAQEIASNSSHVPMTTSTTNQRPEKNTHRTSAYEIAASAATYVQFQAGGLVNFESEPFHEEVDDSILDKTDSSSRIYNSEMAAYMAASTMTAVVAAPEKEKQEAARDLQSLHSSPCEWYICDDSSIYTRCFVIQGSDSVASWQANLFFEPTEFEGTGVPVHRGIYEAAKGIYEQFMPHILEHLNRYGERAKLQFTGHSLGGSLSLLVNLMLLTRNMVKPSALRPVVTFGSPFVFCNGQKILDKLGLDENHVHCVMMHRDIVPRAFSCNYPNHVAQLLKRLCGTFRSHPCLNRNSFLYTPLGKMFILQPDEKSSPHHPLLPPGSALYAMENTHRGLTKTAIRAFLNSPHPIETLQHPTAYGSDGTILRDHDSSNYLKAVNGIIRQHTKTFIRRPKQQRNLLWPLLTSQSPHHWSQETKIKEKKLTVSDQKRLVTTEVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GCY3</accession>
    <name>A0AAD5GCY3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_032330</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009168">
        <property type="protein sequence ID" value="KAI7736899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GCY3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33870">
        <property type="entry name" value="CARDIOMYOPATHY-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33870:SF4">
        <property type="entry name" value="CARDIOMYOPATHY-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="148"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="440"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="454"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="483"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7736899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="526" mass="58853" checksum="95EBE178559AC2F5" modified="2024-05-29" version="1" fragment="single">MGIEMVRNHISNSVRICYRSASRHPLLVFMVFVLIWTYIWFPLLFTLLLHASPVIASTFLLLGTLLFYGHANAPAIQKEGTSSHHHDIHNLVFGGMGNNEFDEETYVVHNMNINDGHKFGISIDSRVSRKQEGNSELGSRRPVLVNRGGQFQPFETGNPQLKSRDGSRSDLFDPLHSVLGHIDDDDNKGWDSGSDASLADNIPMLDEHLPLLESGGIINETDYDVDDQYDHEDIVVKDDYDDDDETLIRWTEDNQRNLMNMGTLELERNQRLESLVARQKVIKNMRMLAEKNMANISPDIRFMDAHISTAGNNPFDLPNHSHENIIGSAPSNMQPRRHSLQLPYDPHGGKPDQLGPTFQFNSVALPTNLPLGRRQRSSDVGTAGHSSQYLYSAPVRIGSDTESIILTHASDINEHIIPRRRRLSLDGDTVKLNQAAGFEDKTSNKSSSSSFCDLTDDINDGDDNSEEEEFGSVNSPTTPDLKDEQDMDDSGDSYTDTDSDSENDGDSDSDSDEAGFTERLISWFTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GEA8</accession>
    <name>A0AAD5GEA8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_027275</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008666">
        <property type="protein sequence ID" value="KAI7739167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GEA8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31150">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31150:SF42">
        <property type="entry name" value="ZINC FINGER, RING_FYVE_PHD-TYPE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="3">
        <location>
            <begin position="254"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="68"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="136"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="73"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="136"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7739167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="350" mass="39040" checksum="6F605C93F6219A1D" modified="2024-05-29" version="1">MGSACCVAAKDRTITDRSSNDAVTRNDRYSPSWSFRWDNRGRVAGEESSINDRLDNKSHTTVETAYATEGSPLDSSRSLRWQKSPPSEEIGLPQLDMLSSKNLHEVKDSRGTQIPVALELSPTIMLTPAHSISSLSASPLSSSRSQLMPMSSLTPSRWSRRSPGHHHLLRQISDSRIRGMMSPNFSISEDGSPFMHHPGLTNKSNIGSHGGSSDGWSNRDRWSFDSDAMSFSRDRFSRSSVRVSSSPTVEIMTCGICSKLLTERSSWSSHKIMATNGLPVVSVLICGHVYHAECLENMTSEINKYDPACPVCTLGEKKGSYDGVKFQHEKLYKYRKTVFEETFFFRLQTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GEI7</accession>
    <name>A0AAD5GEI7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Chorein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_001541</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009080">
        <property type="protein sequence ID" value="KAI7737218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GEI7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026854">
        <property type="entry name" value="VPS13_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45523:SF2">
        <property type="entry name" value="OS02G0470600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45523">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT (TPR)-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12624">
        <property type="entry name" value="VPS13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Chorein N-terminal" evidence="2">
        <location>
            <begin position="46"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="697"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7737218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="697" mass="78437" checksum="F2864D89ED0025E9" modified="2024-05-29" version="1" fragment="single">MYHDSNQLPWNMEKKWEDLSPKDWVEIFEDGINEPAKGHKFSECAGDRNYLVSPINGVLKYHRLGNSERTNPEMPFEEAHLILGDVSLALTEAQYHDWIRLLEVIKRYKSYIEVSHLRPVVSVTEGPKLWWNYAAQAALQQKKMCYRLSWAQIQHLSSLRRRYIQLYADSLQELSDSDDTEIRSIERDLDPKVILLWRFLAHAKVESVKSKEEAEQRMLKKNSWFSLGWGSASSDPLQQDVPEGSQTVVNGLSKEEWKALNNILSSQPDEDSVSQSGKDMQNMTQRMVIVSIGQAAARIININETELVCGRFEQLQVSTKFKHRSVYCDLTLKFYGLSAPEGPLCQSVSSKHKANALGACFVYSPVGENLDWRLSATISPCHVTVLMESYNHFLEFMKRSSSVSPTVAFETATALQNKLESATRRAQEQFQTVLEEQSSFALDIDLDAPKIRVPIRTSASSEYDSLFLLDFGNFTLRTEEGRHDNDGQNLYSRFCISGRDIAAFFTDGNSNIQTCDVESSGYGSQLSAYPVTGSACHSYSLVDRCGIVVMVDQIKVPHPCHPSTRVSVQVPTLGIHLSPSIFSLLMELLKILSGTIESSTKLVEDYQAEHAPWSSPDLATEVQILVWRGIGYSVASWQSSYLVLSGLYLYLLESSTSQNYQRCYSMAGKQVYEVPSNSVGGSSSCIALCIRGMDIQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GFU1</accession>
    <name>A0AAD5GFU1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DHHA1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_027222</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008325">
        <property type="protein sequence ID" value="KAI7740905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GFU1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.310.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038763">
        <property type="entry name" value="DHH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46922">
        <property type="entry name" value="DHHA1 DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46922:SF4">
        <property type="entry name" value="DHHA1 DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64182">
        <property type="entry name" value="DHH phosphoesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7740905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="335" mass="37108" checksum="F072A2376E938E5B" modified="2024-05-29" version="1">MKAGKEEEELDHRPPLDGGAPPCMAAHEDYPNRMALKQTAVVYHYPCPDGAFAALAAHIFFSHSSLPALFFPNTVYSPIKVEDLPVDQIGDAYLLDFVGPPGFVQQLSPKVDRVIILDHHKTAVKMLEDGEFGSENVAKVIDMGRSGATIAYDYFKEKISALANGNNTSGLLKFEQSRRLFEYIEDADLWRWRLHNSKAFSSGLKDLNLEFSVTLNPLLFQQVINQGVNSLSQKQTLINEAVDADSVSDLRSELGHQLAEKSRNHNLRGIGAVVYRVPELNDDQILKISLRSIDNEDTTSISQKHGGGGHRNASSFMLSCQEFEQWKVCCATSGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GHE2</accession>
    <name>A0AAD5GHE2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">MHD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_033154</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Endomembrane system</location>
            <topology evidence="5">Peripheral membrane protein</topology>
            <orientation evidence="5">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the adaptor complexes medium subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007959">
        <property type="protein sequence ID" value="KAI7742557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GHE2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030119">
        <property type="term" value="C:AP-type membrane coat adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09256">
        <property type="entry name" value="AP_MuD_MHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1170:FF:000027">
        <property type="entry name" value="Adaptor complexes medium subunit family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1170:FF:000025">
        <property type="entry name" value="AP-5 complex subunit mu isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1170">
        <property type="entry name" value="Mu homology domain, subdomain B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036168">
        <property type="entry name" value="AP2_Mu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039591">
        <property type="entry name" value="AP5M1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028565">
        <property type="entry name" value="MHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16082">
        <property type="entry name" value="AP-5 COMPLEX SUBUNIT MU-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16082:SF2">
        <property type="entry name" value="AP-5 COMPLEX SUBUNIT MU-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00928">
        <property type="entry name" value="Adap_comp_sub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49447">
        <property type="entry name" value="Second domain of Mu2 adaptin subunit (ap50) of ap2 adaptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51072">
        <property type="entry name" value="MHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="MHD" evidence="7">
        <location>
            <begin position="312"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="505"/>
            <end position="531"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7742557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="626" mass="68154" checksum="51B0A4718F2DE6DF" modified="2024-05-29" version="1">MNSSCSIRALWILNNQDTVLFSRRFPVVERRWNVAVNRANKRCKGDQGFIVLPSSFPTDSEIAAAFTERKKREGSALGYGIRAKQSVEGSDSWVDDPIMRHIVSLHVKKEENGEDYTFWPLILHAKVQYYILVLPLVEPEHLKAYNTLCRRSDCGNGVGVDGNLSSLLLQLPCITGAFMVAHVLGDIITSDIPEPEVVVSAGPSVGGLLDSLTGSIGISARPKPVAPPVAASTNSTAIGGGSLASDAPKTGPRNLDKDALRSFISTAMPFGTPLDLNYSNITAIRTNGFSPADVAPVDSKQPAWKPYLYKGKQRLLFTIHEIVNAAMYDRDEIPDSISVLGQVNCRAELEGLPDVLFPLTGLDSKRIEILSFHPCAQVPEQGGGDKQAVMFSPPLSNFVLLRYQAPCSIGVPVKGFYQLSMVSENEGAFLFKLSLMEGYKAPLTMEFCTVNMPFPRRKVISFEGTPSIGTVSYTKHSVEWKIIPSGRGVGAKSIEATFPGTVKFAPWKGQSSDDSGSVQGGDEDSDTEIEQGNSMVNVEEYLVEKMNKHLPAVDLEEPFCWHAYNYAKVSFKMAGTSLAGMMIDTKSVNIFPSVKVPVEYSTQVHSGDYILWNTLGKCPVAATPAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GIA3</accession>
    <name>A0AAD5GIA3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">M8C21_007769</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">AA19_3_7</strain>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Vacuole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase C1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007693">
        <property type="protein sequence ID" value="KAI7743755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GIA3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010623">
        <property type="term" value="P:programmed cell death involved in cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02248">
        <property type="entry name" value="Peptidase_C1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000055">
        <property type="entry name" value="cysteine protease XCP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025660">
        <property type="entry name" value="Pept_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039417">
        <property type="entry name" value="Peptidase_C1A_papain-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013201">
        <property type="entry name" value="Prot_inhib_I29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08246">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00705">
        <property type="entry name" value="PAPAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00848">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00639">
        <property type="entry name" value="THIOL_PROTEASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0926">Vacuole</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041996607" evidence="12">
        <location>
            <begin position="24"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="Cathepsin propeptide inhibitor" evidence="14">
        <location>
            <begin position="46"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="13">
        <location>
            <begin position="133"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAI7743755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="351" mass="39262" checksum="4F000AE55139F172" modified="2024-05-29" version="1">MTLSLKSPLLVFFLALFACSAFGRDLSIVGYAPEDLTCIDKVINLFESWVSKHAKFYESVEEKLHRFEIFKDNLKHIDETNKKVSNYWLGLNEFADLTHEEFKSMFLGLKGEFPQRRDHSSPQDFTYTDFVNLPKSVDWRKKGAVVPVKNQGSCGSCWAFSTVAAVEGINQIVTGNLTELSEQELIDCDTSFNNGCNGGLMDYAFSFIVRNGGLHKEEEYPYIMSEGTCDEMKDVSEKVTISGYHDVPQNDEQSFLKALANQPISVAIDASGRDFQFYSGGVFDGHCGTDLDHGVAAVGYGTSKGVDYVIVRNSWGPKWGEKGYIRMKRKTGKSEGMCGLYKMASYPTKPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GJV7</accession>
    <name>A0AAD5GJV7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Rac-like GTP-binding protein RAC2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_031559</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the small GTPase superfamily. Rho family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007202">
        <property type="protein sequence ID" value="KAI7745555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GJV7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04133">
        <property type="entry name" value="Rop_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000535">
        <property type="entry name" value="rac-like GTP-binding protein RAC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0636">Prenylation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7745555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="196" mass="21845" checksum="87987DBBAE9A9732" modified="2024-05-29" version="1">MAAARFIKCVTVGDGAVGKTCMLISYTSNTFPTDYVPTVFDNFSANVVVGESTVNLGLWDTAGQEDYNRLRPLSYRGADVFLLAFSLISRPSYENISKKWISELRHYAPTVPIVLVGTKLDLREDKQYLSDHPNVTPITTSQGEELKKTIGAAVYIECSSKTQQNVKAVFDAAIRVVLQPPKMKKKRSKQRLCVYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GME5</accession>
    <name>A0AAD5GME5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TF-B3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_034015</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006537">
        <property type="protein sequence ID" value="KAI7748377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GME5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd10017">
        <property type="entry name" value="B3_DNA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.330.10">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003340">
        <property type="entry name" value="B3_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015300">
        <property type="entry name" value="DNA-bd_pseudobarrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050655">
        <property type="entry name" value="Plant_B3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31920">
        <property type="entry name" value="B3 DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31920:SF122">
        <property type="entry name" value="B3 DOMAIN-CONTAINING PROTEIN REM23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02362">
        <property type="entry name" value="B3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01019">
        <property type="entry name" value="B3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101936">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50863">
        <property type="entry name" value="B3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TF-B3" evidence="6">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="TF-B3" evidence="6">
        <location>
            <begin position="117"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7748377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="223" mass="25548" checksum="446210A260CA56F7" modified="2024-05-29" version="1">MLLAQSSPRLPLSPDFVNKLLEDKIQQGPIMLTTKGGQSWRLEIKQIDDTYYFTNGWNNVVEDMQLRFGDFLSFRPLDQSTIKMSIFSPKDCDRFLAPKVITHDHGGNGKNEDGDPFFTSKINKNHIRSRVLRLPIEFARLAGIDGEGSMRVKNLDGKECVMGLKLDKSFRGKERYMLTSGWPQFLQENELSEGDECVFKFIRSEGKLQLAKVTKKQLKYGFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GN69</accession>
    <name>A0AAD5GN69_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Diacylglycerol kinase</fullName>
            <shortName evidence="12">DAG kinase</shortName>
            <ecNumber evidence="12">2.7.1.107</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">M8C21_011091</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">AA19_3_7</strain>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a 1,2-diacyl-sn-glycerol + ATP = a 1,2-diacyl-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10272"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.107"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the eukaryotic diacylglycerol kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.107" evidence="12"/>
    <dbReference type="EMBL" id="JAMZMK010006422">
        <property type="protein sequence ID" value="KAI7748960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GN69"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004143">
        <property type="term" value="F:ATP-dependent diacylglycerol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00029">
        <property type="entry name" value="C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.40:FF:000006">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10330:FF:000006">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037607">
        <property type="entry name" value="DGK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000756">
        <property type="entry name" value="Diacylglycerol_kin_accessory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001206">
        <property type="entry name" value="Diacylglycerol_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11255">
        <property type="entry name" value="DIACYLGLYCEROL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11255:SF54">
        <property type="entry name" value="DIACYLGLYCEROL KINASE THETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00609">
        <property type="entry name" value="DAGK_acc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00781">
        <property type="entry name" value="DAGK_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00045">
        <property type="entry name" value="DAGKa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00046">
        <property type="entry name" value="DAGKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50146">
        <property type="entry name" value="DAGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0346">Stress response</keyword>
    <keyword evidence="4 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="26"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="DAGKc" evidence="15">
        <location>
            <begin position="362"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="263"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="287"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="303"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAI7748960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="733" mass="80682" checksum="929C594F380014E2" modified="2024-05-29" version="1">MDDYSQPEILPPAWIKKNPSELAESWIFIFSCFVAGIIGILTIVYTAFQFRRNNSLSLMKATARKKKDPKSRKKVPLAAHTWYLESGSRGKNLNCCVCLKSVSYSQALGPVVASDSFIHRCSICGAAAHLSCSKNAHRDCKCVSMIGATHVLHLWAIRSTEVVDQPDEISYCSHCEEPCSASFIGGSPIWCCLWCQRLVHVDCHSGFYSDTGDVCDLGPFRRLIVSPLHVKELSRSSSGGILSSITHGANEIASSVRATIISQSKKTKQGNEVSMERSNGVVRESSTESTADSTLANDVSNRTQENQNGNTNTEGTSPNQDNNVVNKLGRKLSFKRSLSNSQRDESSVAGMKQKYELMDLGPDARPLLVFINKKSGAQRGDSLRLRMNILLNPVQVFELSSIEGPEVGLYLFRKVANFRILVCGGDGTVGWVLDAIEKQNFVSPPPVAILPAGTGNDLARVLGWGGGLGSVERQGGLCTMLQDIEHAALTILDRWKISITTQKGKQLRAPKFMNNYLGVGCDAKVALEIHNLREENPEKFYNQFMNKVLYAREGARTIMDRTFADYPWQVKVEVDGVDVEVPEDAEGVLVVNIGSYMGGVDLWQNEDDNYDNFDPQSMHDKRLEVVSISGTWHLGKLQVGLSRARRLAQGKTIKIQLLAQLPVQIDGEPWMQSPCTLTISHHGQAFMLKRSSEEPLGHAAAIVADVLAHAETNNVISASQKRTLLQEMALKLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GNW2</accession>
    <name>A0AAD5GNW2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">4-hydroxybenzoate octaprenyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_008915</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UbiA prenyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006306">
        <property type="protein sequence ID" value="KAI7749695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GNW2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016765">
        <property type="term" value="F:transferase activity, transferring alkyl or aryl (other than methyl) groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1780">
        <property type="entry name" value="UbiA prenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039653">
        <property type="entry name" value="Prenyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11048:SF28">
        <property type="entry name" value="4-HYDROXYBENZOATE POLYPRENYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11048">
        <property type="entry name" value="PRENYLTRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7749695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="68" mass="7545" checksum="06ED1882DAD9D6D8" modified="2024-05-29" version="1">MYLYKRCLAFNKAGLPYYVFLAAASGQLAWQIWTVDISSAADCSRKFVSNKWFGALVFGGILMGRLSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GRB8</accession>
    <name>A0AAD5GRB8_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_020680</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005682">
        <property type="protein sequence ID" value="KAI7752457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GRB8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="132"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="132"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7752457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="156" mass="17075" checksum="6E6E4E9AE297C3EC" modified="2024-05-29" version="1">MASVEVEPVTNNLQEKVESTHEASKEIEPIVATPPAPEAEVEEPTKEPLTIDAPALVEEPPVIQDPIETETKAMEEPKNEVEEVKAEATQEEVKQNEESKKETLATTDVVAEETSATIDAVAKETQTSVTEEVKKHVKEDEKSDEVVASTEVAKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GSK2</accession>
    <name>A0AAD5GSK2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glucose-6-phosphate dehydrogenase (NADP(+))</fullName>
            <ecNumber evidence="3">1.1.1.49</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_010541</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>D-glucose 6-phosphate + NADP(+) = 6-phospho-D-glucono-1,5-lactone + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15841"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57955"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:61548"/>
            <dbReference type="EC" id="1.1.1.49"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glucose-6-phosphate dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.49" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010005426">
        <property type="protein sequence ID" value="KAI7753475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GSK2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004345">
        <property type="term" value="F:glucose-6-phosphate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009051">
        <property type="term" value="P:pentose-phosphate shunt, oxidative branch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001282">
        <property type="entry name" value="G6P_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019796">
        <property type="entry name" value="G6P_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022674">
        <property type="entry name" value="G6P_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23429:SF0">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE 1-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23429">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE 1-DEHYDROGENASE G6PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00479">
        <property type="entry name" value="G6PD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00079">
        <property type="entry name" value="G6PDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00069">
        <property type="entry name" value="G6P_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-0313">Glucose metabolism</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="domain" description="Glucose-6-phosphate dehydrogenase NAD-binding" evidence="10">
        <location>
            <begin position="48"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7753475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="91" mass="10461" checksum="0AC6F820D2CDF928" modified="2024-05-29" version="1">MDNDQVTIDVASGRFRMPMNRLSSPFVTIRLLFVKLILVVGLELLLRFEKPFGWDLASAEELSAHIGELFDEPQIYRIDHYLGKELVQNLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GSX2</accession>
    <name>A0AAD5GSX2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_021310</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005949">
        <property type="protein sequence ID" value="KAI7751241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GSX2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016603">
        <property type="term" value="F:glutaminyl-peptide cyclotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007788">
        <property type="entry name" value="QCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31270">
        <property type="entry name" value="GLUTAMINYL-PEPTIDE CYCLOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31270:SF1">
        <property type="entry name" value="GLUTAMINYL-PEPTIDE CYCLOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05096">
        <property type="entry name" value="Glu_cyclase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7751241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="95" mass="10647" checksum="03C89609BB83AAC4" modified="2024-05-29" version="1" fragment="single">MKDGWGLATDGKILFGTDGSSSLYHLHPRTMKVIKKHVMKYKGHESDCIARISPADGSVIGWVLLPELRAELLSVGNRVSIHIHELIIASCYYWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GTD3</accession>
    <name>A0AAD5GTD3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">X8 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_009482</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010002666">
        <property type="protein sequence ID" value="KAI7754722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GTD3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009506">
        <property type="term" value="C:plasmodesma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012946">
        <property type="entry name" value="X8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044788">
        <property type="entry name" value="X8_dom_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31044">
        <property type="entry name" value="BETA-1,3 GLUCANASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31044:SF71">
        <property type="entry name" value="MAJOR POLLEN ALLERGEN OLE E 10-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07983">
        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00768">
        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041987046" description="X8 domain-containing protein" evidence="2">
        <location>
            <begin position="27"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="X8" evidence="3">
        <location>
            <begin position="29"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7754722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="107" mass="11530" checksum="6805F3405C1A09B8" modified="2024-05-29" version="1">MAVQLSTSVILLLAFLSIGTLRSVHAQKIWCVAKPSTSEATLLENINFACSQVDCVILQKGGACYSPDNVFNHASIAMNLYFQSKGGNNWNCDFKSSGLLTVSNPSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GTL1</accession>
    <name>A0AAD5GTL1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidase S8/S53 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_018234</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the peptidase S8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010002609">
        <property type="protein sequence ID" value="KAI7754735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GTL1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045051">
        <property type="entry name" value="SBT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10795">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0720">Serine protease</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Peptidase S8/S53" evidence="7">
        <location>
            <begin position="49"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7754735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="152" mass="16774" checksum="40AE0303E79F5504" modified="2024-05-29" version="1" fragment="single">MNAHPFIMEWILLPFSFIPIKITDRLIDNSFGSGLHRWAGVMGRPSGLETDKRRVKFNILSGTSMSCPHVSGVATWLMAQNPSWSPAAIKSAIMTTAYIHDNTFDPLTDSSTGSPSGPYDHGAGHINPPRALDPGLVYDIEPQHYFDFLCTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GTL6</accession>
    <name>A0AAD5GTL6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_023445</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005931">
        <property type="protein sequence ID" value="KAI7751348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GTL6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="15"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7751348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="82" mass="9190" checksum="190595366AA62C77" modified="2024-05-29" version="1">MATVAAAARSIFRSSSVRSTAARASSQAKAGRSPFRIGTQNNSLSNRIFRYLTNRHRSLLDLLSFDLEALTRLDEDRETKME</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GU42</accession>
    <name>A0AAD5GU42_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_002615</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005794">
        <property type="protein sequence ID" value="KAI7751888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GU42"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7751888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="96" mass="10637" checksum="DB615DF7A0754B16" modified="2024-05-29" version="1">MEDDTGERSSFVIGLIENRAKEVGVAAFDLRSASLHLSEYIETSSSYQNTKTLLHFYDPIVIIVPPNKLAPDGMVGVSELVDKFYSSIRKVISIIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GVS6</accession>
    <name>A0AAD5GVS6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Serine aminopeptidase S33 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_014729</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010004484">
        <property type="protein sequence ID" value="KAI7754106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GVS6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42886:SF82">
        <property type="entry name" value="FERULOYL ESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42886">
        <property type="entry name" value="RE40534P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine aminopeptidase S33" evidence="1">
        <location>
            <begin position="75"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7754106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="305" mass="34200" checksum="178D7D953513D5ED" modified="2024-05-29" version="1">MFCLSSCSVLLPAASKSLKIGLHINNQSHSLQYLKTEMATTTNSLHLNPGVEQKRIVIQNKCCEKLVGLLHESASKQIVILCHGFRDSKNYSMMVELAFALEKHGISAFRFDFSGNGESEGLFQFGNYSKEVDDLHAVIQHFTAENRVISAILGHSKGGNVVVLYASLHHDVHTVINVSGRYKMDRGIEERLGKDYLERVKRDGFIDIKSKQGAFLFRVTEESLMERLNTNMHEAGLKVDPSCKVLTVHGSVDEVIPVEDALQFDKIIPNHELRIIEGADHGYNNHRDELLSVVLAFLKDNMLRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GWP8</accession>
    <name>A0AAD5GWP8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Tryptophan synthase beta chain-like PALP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_014667</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cysteine synthase/cystathionine beta-synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000008">
        <property type="protein sequence ID" value="KAI7758305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GWP8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019344">
        <property type="term" value="P:cysteine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000006">
        <property type="entry name" value="Cysteine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050214">
        <property type="entry name" value="Cys_Synth/Cystath_Beta-Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10314">
        <property type="entry name" value="CYSTATHIONINE BETA-SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0198">Cysteine biosynthesis</keyword>
    <keyword evidence="6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="8">
        <location>
            <begin position="3"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7758305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="166" mass="17562" checksum="8A1F8AD802AC6F88" modified="2024-05-29" version="1" fragment="single">IHYETTGPEIWKASEGKVDAFVSGIGTGGTVTGVGKFLKEKNADIKIYGVEPSESAVLNGGNPGPHKIQGIGNGIIPDVLDVRVLDEVIMITSDEAIETAKLLALKEGLLVGISSGAAAAAAIKIAKRPEYEGKLIVVVFPSFGERYLSTELFDSVRKEVENLPIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GXG1</accession>
    <name>A0AAD5GXG1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 18">Adenine DNA glycosylase</fullName>
            <ecNumber evidence="5 18">3.2.2.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">M8C21_012499</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">AA19_3_7</strain>
            <tissue evidence="21">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Adenine glycosylase active on G-A mispairs.</text>
    </comment>
    <comment type="function">
        <text evidence="17">Involved in oxidative DNA damage repair. Initiates repair of A*oxoG to C*G by removing the inappropriately paired adenine base from the DNA backbone. Possesses both adenine and 2-OH-A DNA glycosylase activities.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 18">
            <text>Hydrolyzes free adenine bases from 7,8-dihydro-8-oxoguanine:adenine mismatched double-stranded DNA, leaving an apurinic site.</text>
            <dbReference type="EC" id="3.2.2.31"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="18">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 18">Belongs to the Nth/MutY family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.31" evidence="5 18"/>
    <dbReference type="EMBL" id="JAMZMK010000823">
        <property type="protein sequence ID" value="KAI7755771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GXG1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034039">
        <property type="term" value="F:8-oxo-7,8-dihydroguanine DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035485">
        <property type="term" value="F:adenine/guanine mispair binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032357">
        <property type="term" value="F:oxidized purine DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000701">
        <property type="term" value="F:purine-specific mismatch base pair DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03431">
        <property type="entry name" value="NUDIX_DNA_Glycosylase_C-MutY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1670.10:FF:000002">
        <property type="entry name" value="Adenine DNA glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.30:FF:000002">
        <property type="entry name" value="Adenine DNA glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.79.10:FF:000026">
        <property type="entry name" value="Adenine DNA glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.10">
        <property type="entry name" value="Helix-hairpin-Helix base-excision DNA repair enzymes (C-terminal)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.30">
        <property type="entry name" value="Hypothetical protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023170">
        <property type="entry name" value="HhH_base_excis_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000445">
        <property type="entry name" value="HhH_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044298">
        <property type="entry name" value="MIG/MutY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029119">
        <property type="entry name" value="MutY_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42944">
        <property type="entry name" value="ADENINE DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42944:SF1">
        <property type="entry name" value="ADENINE DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00633">
        <property type="entry name" value="HHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14815">
        <property type="entry name" value="NUDIX_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="DNA-glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="9 18" id="KW-0227">DNA damage</keyword>
    <keyword evidence="14" id="KW-0234">DNA repair</keyword>
    <keyword evidence="16 18" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11 18" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HhH-GPD" evidence="20">
        <location>
            <begin position="108"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="7"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00058024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU365096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="SMART" id="SM00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAI7755771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="464" mass="52599" checksum="5C89052BC2E7BD3C" modified="2024-05-29" version="1">MKKKKANNTTPPITDTITKSNKRPRRACQLQPKPEPEPEPEPETTFTIGDIEDFKFRVEEVTEIRTSLLTWYDINKRDLPWRRISEGEANTDDEIDRKAYAVWVSEIMLQQTRVQTVIDYFNKWMVKWPTVHHLAQASIEEVNEMWAGLGYYRRARFLLEGAKMVVEEGGKFPRSVNDLRRVKGIGEYTAGAIASIAFKEAVPVVDGNVIRVIARLKTISANPKDKTTIKSIWRLAGQLVDGHRPGDFNQAVMELGATLCTPSTPNCSSCPVSSQCSALLKTKNDPSALVTDYPTKVAKVKQRHDYSAVTVVEIIENEQGKVNTSDSGSKFLIVKRPEEGLLAGLWEFPSVSLNEKDDLVTRRRAVDDFLKNSFGLNINRSSSIVSRDEVGEYVHIFTHIRLKMYIELLVLRSQDLHRKKNKGLMSFKYVDSETLATMGLSTGVRKAYNMVQKFRQNKSSPISR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GYK1</accession>
    <name>A0AAD5GYK1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">mitogen-activated protein kinase kinase kinase</fullName>
            <ecNumber evidence="2">2.7.11.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_002346</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.25"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.25"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.25" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010000459">
        <property type="protein sequence ID" value="KAI7756313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004709">
        <property type="term" value="F:MAP kinase kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd06606">
        <property type="entry name" value="STKc_MAPKKK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000387">
        <property type="entry name" value="Serine/threonine-protein kinase STE11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050538">
        <property type="entry name" value="MAP_kinase_kinase_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48016">
        <property type="entry name" value="MAP KINASE KINASE KINASE SSK2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48016:SF56">
        <property type="entry name" value="MAPKK KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="75"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="497"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7756313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="635" mass="70462" checksum="58BE5BA0CE70906F" modified="2024-05-29" version="1">MQDFIVSVRRSLVFKPSPTNDDGIGTSFGGFVEKLGSSIRKSKIGLFSKSSAVQALPAPRTVRMKKEEAGSQIRWRKGELIGCGAFGRVYMGMNLDSGELLAVKQVSVVVNTASKDKTQAHIRELEEEVKLLKNLSHPNIVRYLGTAREEESLNIFLEFVPGGSISSLLGKFGSFPESVIRMYTKQILLGLEYLHKNGIMHRDIKGANILVDNKGQIKLADFGASKKVVELXXXMLFVAALFHIGTTKAHPPIPDHLSSDAKEFLLKCLQKEPDLRSNASELLQHPFVIGEYKETHPVFRTSVVDTSGNQIATARQEPRNPISPETTTKCGTANAHSINSVRCSTIYPEKFSGGPMWGSNRYDDDMCQLDDDIMAGTSMKLDSSLLTNNVNKSFNPMNEPDDDWSYQIDGSSEVGTKAANLFSDQVNEITTDIPGELGNEDNDFTFPNGTPGVEEEEEVTEIKIRAFLDEKALDLKKMQTPLYEEFYNSLNAAISPVGNENKENSPSHLQLPPKSKSPNSNNRLLRKRLSSAVDVSYIGRSANKSDRVSNIGGVNAPNPHECLSPQHGQVNENILDSQKHAISPSASFSERQRRWKEELAEELERKRELMRQAGTSSPKDRIINNRQKRFAFLGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GZ92</accession>
    <name>A0AAD5GZ92_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_016492</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000097">
        <property type="protein sequence ID" value="KAI7757601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GZ92"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7757601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="103" mass="11126" checksum="751DF96E9EDE5940" modified="2024-05-29" version="1">MATGDDAPTGRSGGKILKDKRIASRKTPYDRPQPHHPQETPNWLTGLVSPAKFVAGSASKIISSIWNPNTWASRSSDTDSDSVRVCYKALKHVWAPPADSSAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GZW9</accession>
    <name>A0AAD5GZW9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_014653</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000008">
        <property type="protein sequence ID" value="KAI7758291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GZW9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35300">
        <property type="entry name" value="COACTIVATOR CBP, KIX DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35300:SF5">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7758291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="84" mass="9682" checksum="8F8C185E43B39035" modified="2024-05-29" version="1">MYSKANSQTKYMNLETLWDRANDAINTIFRLDESTETGVFLQPCIEVHENCHLALVCGFQETQSWMIALHEDDNINDATRTRGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BK25</accession>
    <name>A0AAD5BK25_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_006389</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012174">
        <property type="protein sequence ID" value="KAI7724655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BK25"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38360:SF1">
        <property type="entry name" value="F12P19.7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38360">
        <property type="entry name" value="OS03G0120000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53807">
        <property type="entry name" value="Helical backbone' metal receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042278167" evidence="1">
        <location>
            <begin position="29"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7724655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="294" mass="33187" checksum="469A39332BEE72E0" modified="2024-05-29" version="1">MLLMYLAVLSSALGGLLILNGGVMVAEGAAVQVTGNISKIEDASYFRIYYGKTFKVIKNDLDGKSYLLIQSDSKMAIKTKYCTPRIKSFVIPLANFSMDVSYFPVSFFERAEWIKYLGVFANVETRANEIYDVVKSNYQCLVKSAASKKIRPIVAWMEFNDGVWTFTKDAYKLKTVDVLIDGTLTQDPFDYNGTMFLQNLNIEDQSCLAFLSHESVWRHDKRLSTDMALDWFDGAVSQPQLVLADMIEMLFPTGSYTTTYFRNLLKGEEVTILGLENCNRDTFTAMEPTTIACT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BK69</accession>
    <name>A0AAD5BK69_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">40S ribosomal protein S7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_004051</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012168">
        <property type="protein sequence ID" value="KAI7724757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BK69"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000554">
        <property type="entry name" value="Ribosomal_eS7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047861">
        <property type="entry name" value="Ribosomal_eS7_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11278">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11278:SF30">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01251">
        <property type="entry name" value="Ribosomal_S7e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00948">
        <property type="entry name" value="RIBOSOMAL_S7E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU364105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7724757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="191" mass="21948" checksum="2E0AAB35BD5F5599" modified="2024-05-29" version="1">MYTSRKKIHKDKDVEPTEFEDSVAQALFDLENANQDLKSDLKDLYINSATQIDVSGNRKAVVIHVPYRLRKAFRKIHLKLVRELEKKFSGKDVVLIATRRMLPPPKKGSAAQRPRNRTLTAVHDAMLEDIVYPAEIVGKRVRYRVDGSKVIKIFLDPKARNDTEYKLETFGGVYKKLCGKDVVFEYPTTEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BLA9</accession>
    <name>A0AAD5BLA9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_000087</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011969">
        <property type="protein sequence ID" value="KAI7725386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BLA9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004252">
        <property type="entry name" value="Probable_transposase_24"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03004">
        <property type="entry name" value="Transposase_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7725386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="140" mass="16663" checksum="D219A6DBB811F562" modified="2024-05-29" version="1" fragment="single">MWSDPWQGWKDVPSHHRKRLFERFQQYYRWEDRSESLIYSCWEKCIKGKFPDLLKRARDKAKTLADQEDIELGNDLTPILPFKPLRISQEYWETLVEAWNTDSWKGKSSQNSENRGKAIGGRHTLGSKSFATVRKNMVRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BLJ2</accession>
    <name>A0AAD5BLJ2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_010493</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011921">
        <property type="protein sequence ID" value="KAI7725535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BLJ2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7725535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="125" mass="14667" checksum="AFE04A6D896A834A" modified="2024-05-29" version="1" fragment="single">MNDDFNSVTIGFPRNPIFILSPVEARTRYAFPLFLVAALHFLKMNLERWRHTDQNIFCWRMNAIVIEKYLGRYSFITNFEPTKSWQFALCMLEHGEVDIDGSLVVANWLERDRTVRVMVTATSCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BLN6</accession>
    <name>A0AAD5BLN6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">M8C21_020251</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">AA19_3_7</strain>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Responsible for microtubule translocation. May be important for the organization of phragmoplast-specific arrays of microtubules. Plays an essential role in stabilizing the mitotic spindle. Required during mitotic cytokinesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Spindle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. KIN-5/BimC subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011941">
        <property type="protein sequence ID" value="KAI7725429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BLN6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005876">
        <property type="term" value="C:spindle microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008574">
        <property type="term" value="F:plus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090307">
        <property type="term" value="P:mitotic spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051231">
        <property type="term" value="P:spindle elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01364">
        <property type="entry name" value="KISc_BimC_Eg5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000019">
        <property type="entry name" value="Kinesin-like protein KIN-5D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047149">
        <property type="entry name" value="KIF11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047241">
        <property type="entry name" value="KIF11-like_kin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47970">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIF11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47970:SF9">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIN-5D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3 11" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6 10 11" id="KW-0505">Motor protein</keyword>
    <keyword evidence="4 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="14">
        <location>
            <begin position="45"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="403"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="9"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="131"/>
            <end position="138"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00046159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAI7725429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="865" mass="97499" checksum="73DE0332454322A9" modified="2024-05-29" version="1">MEKKGANAIPSSPSQTPRSVEKSARDIRHVEGNMSGKHDKEKGVNIQVVVRCRPMNADEERLHTPVVVTCAENKREVCAVQNIANKQIDRSFVFDKVFGPKSEQRDLYHSAVSPIVFEVLEGYNCTIFAYGQTGTGKTYTMEGGGRKKNGEFPSDAGVIPRAVRQIFDILEAQSAEYSMKVTFLELYNEEITDLLAPEEVSKFPEDKSKKPIALMEDGKGGVLVRGLEEEMVSTADEIYRILEKGSAKRRTAETLMNKQSSRSHSIFSITIHIKERTPEGEEVIRCGKLNLVDLAGSENISRSGAKEGRAREAGEINKSLLTLGRVINTLVERSGHIPYRDSKLTRLLRDSLGGKTKTCIIATISPSIHSLEETLSTLDYAHRAKNIKNKPEINQKMMKSAMMKDLYSEIERLKQEVYAAREKNGIYIPKDRYLQEEEEKKAMAEKIERMELDNESRGKQMMELQELYNSQQLITANLTEKLGKTEVKLQETEHSMFDLEEKHRQANVTIKEKEFLISNLLKSEKALLERAFDLRGELENAASDVSRLFTKIERKDKIEDENRILVQQFQSQLTNQLDTLHKTIAASVTQQEQQLKGMEEDMKSFVSTKAEATEELRAALEKLKTMYGSGIKALDDIADELDGNSQSTFGLLNSQVSKNSSALGELFKGIASEAEKLLGDLESILYSQENKLTAFAQQQREAHSRAVETSRTISKTTMNFFDTLDMHATKLIKIVEEGQTVNDQKLSELERKFEECAAIEEKQLLEKVAELFAVSNARKKELVRTAVDGLRETAASKAKRFDQEMSTMKDSSSCIRNDWINYTNKTESHYLEDTAAVESGKADLDEFAKDENGVTTMEQRSGVFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BNX6</accession>
    <name>A0AAD5BNX6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_012346</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011499">
        <property type="protein sequence ID" value="KAI7726872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BNX6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7726872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="80" mass="9174" checksum="E5ED23A408C2E090" modified="2024-05-29" version="1" fragment="single">FDDAKMIEEKGGKLCAKGKMEFAEEELMNEFLYTEINLTQGPKRFLLIEDQLIALTGYLSEAPLYNSMPMTMGRLQIINH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BP38</accession>
    <name>A0AAD5BP38_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box/LRR-repeat protein 15-like leucin rich repeat domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_011377</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011475">
        <property type="protein sequence ID" value="KAI7726952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BP38"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057207">
        <property type="entry name" value="FBXL15_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25372">
        <property type="entry name" value="DUF7885"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box/LRR-repeat protein 15-like leucin rich repeat" evidence="1">
        <location>
            <begin position="43"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF25372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7726952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="127" mass="14495" checksum="F398AD5A5BB7A0AF" modified="2024-05-29" version="1">MATIISKLDVSSICAVTLISKTFRISTQDIFHFLPNFHLLEIAAPIDRLRRLLVPNASLRSLKLDCRRLNESSIDCILQPGLVELSLRNCYKFSGKLLSEVGARCKHLRLLSTCLYNLILYRLFKCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BQ10</accession>
    <name>A0AAD5BQ10_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">low-specificity L-threonine aldolase</fullName>
            <ecNumber evidence="9">4.1.2.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_008009</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Threonine aldolase involved in threonine degradation to glycine. May play a role in the removal of L-allo-threonine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-allo-threonine = acetaldehyde + glycine</text>
            <dbReference type="Rhea" id="RHEA:26209"/>
            <dbReference type="ChEBI" id="CHEBI:15343"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:58585"/>
            <dbReference type="EC" id="4.1.2.48"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:26210"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-threonine = acetaldehyde + glycine</text>
            <dbReference type="Rhea" id="RHEA:19625"/>
            <dbReference type="ChEBI" id="CHEBI:15343"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="EC" id="4.1.2.48"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:19626"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="8">Amino-acid degradation; L-threonine degradation via aldolase pathway; acetaldehyde and glycine from L-threonine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the threonine aldolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.48" evidence="9"/>
    <dbReference type="EMBL" id="JAMZMK010011702">
        <property type="protein sequence ID" value="KAI7726353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BQ10"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008732">
        <property type="term" value="F:L-allo-threonine aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006545">
        <property type="term" value="P:glycine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006567">
        <property type="term" value="P:L-threonine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000041">
        <property type="entry name" value="Low-specificity L-threonine aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000063">
        <property type="entry name" value="probable low-specificity L-threonine aldolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001597">
        <property type="entry name" value="ArAA_b-elim_lyase/Thr_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023603">
        <property type="entry name" value="Low_specificity_L-TA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041359">
        <property type="entry name" value="GntG_guanitoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007825">
        <property type="entry name" value="PRK10534.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48097:SF9">
        <property type="entry name" value="L-THREONINE ALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48097">
        <property type="entry name" value="L-THREONINE ALDOLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01212">
        <property type="entry name" value="Beta_elim_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017617">
        <property type="entry name" value="Thr_aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aromatic amino acid beta-eliminating lyase/threonine aldolase" evidence="11">
        <location>
            <begin position="7"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="10">
        <location>
            <position position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00051558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00052637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00058325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00060555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00066573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR017617-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7726353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="359" mass="38523" checksum="91323099E580ECD4" modified="2024-05-29" version="1">MVTRTIDLRSDTVTKPTETMRLAMAKAEVDDDVLVYDPTARQLEIEMARITGKEAALFVPSGTMGNLISVLVHCEIRGSEVILGDNSHIHIYENGGISTIGGVHPRPIKNNEDGTMDVGLIEAAIRNPDFEICYPKTRLICLENSHANTGGRCLSVEYTDQVGELAKKHGLKLHIDGARIFNASAALKVPVDRLVQAADSVSVCLSKGLGAPVGSVIVGTEAFINRARILRKTLGGGMRQVGVLCAAALVALQENVPKLVTDHKNAKTLAEGLDKIEGLSVDIASVETNIVYFDILKGSRICALKLGKIMEEHGILLMPDSSSRVRIVIHHQVSASDVQYTLSCIKQAITGVTDENGGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BQ81</accession>
    <name>A0AAD5BQ81_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_007689</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NUP186/NUP192/NUP205 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011358">
        <property type="protein sequence ID" value="KAI7727505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BQ81"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021827">
        <property type="entry name" value="Nup186/Nup192/Nup205"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31344">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31344:SF0">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11894">
        <property type="entry name" value="Nup192"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7727505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="244" mass="27907" checksum="440AB8A28127646C" modified="2024-05-29" version="1">MLRIKEEEDEGGFSRWCGRDCLRSRLGSKFSDEVEFCGTMGFLLVYWLWVMENTGILTLQPKGNEPSLEMRVVRKEVASRKWSRESTDQQWTQNCASTHAIKYKPPKASDRAQVESKEVKLPDSGLIPLDDQDVEIALKLSDDLHLNEIDCVRLLASANQEAIVLDQGLKADLQADVLKYVEDLINSELRQRLISLIKLRIKLVALIASQNDKYCMPTNRQLKPKQYMLRVLGHEDVMKMVASP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BRS8</accession>
    <name>A0AAD5BRS8_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_033379</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011407">
        <property type="protein sequence ID" value="KAI7727251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BRS8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7727251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="38" mass="4390" checksum="3E07B2747A60C493" modified="2024-05-29" version="1">MSSTASQKQDPVTFSYQELDWDSHRAEEVDLCILSIRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BST3</accession>
    <name>A0AAD5BST3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_023806</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011132">
        <property type="protein sequence ID" value="KAI7728827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BST3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="90"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7728827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="133" mass="14637" checksum="6996EFEC4A687E3B" modified="2024-05-29" version="1">MDTEPLGTVAAASSAASEPTGTSIDATLFDKLTNQLLNKTCDLVLEMSNPYDAFQEIENQDAQFHVQIQKDKETRCTVSKVYVLNKEQETGSSTPSEPSTPAPKARDTASIKRSMMSPDDEATVDKRKRHKTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BUE7</accession>
    <name>A0AAD5BUE7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Kinesin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_028393</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010986">
        <property type="protein sequence ID" value="KAI7729509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BUE7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968">
        <property type="entry name" value="CENTROMERE PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968:SF33">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIN-7C, MITOCHONDRIAL ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0505">Motor protein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="188"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="Kinesin motor" evidence="4">
        <location>
            <begin position="1"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7729509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="234" mass="25519" checksum="95AF038CAC914BD0" modified="2024-05-29" version="1">MHGEQKSPGIIPLAIKDVFGIIQEVINDLLNPTGQNLTIGEDSQGTYVEGIKEEVVPSPAHALSLIASREAHRHVGSNNFNLLSSRSHTIFTLMIESSPCGENFEEEVTLSRLHLIDLAGFESSKTETTGMRRKEGSYINKSLLTLGTVISKLTDGKSAHISYQDSKLTRLLQSSLSGHGRVFVTSKIFFVVFGSLSATSICVWFVLALHYGHDANCGLGYEALMNVCAQFGFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BV81</accession>
    <name>A0AAD5BV81_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_023099</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010821">
        <property type="protein sequence ID" value="KAI7730262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BV81"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003872">
        <property type="term" value="F:6-phosphofructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047334">
        <property type="term" value="F:diphosphate-fructose-6-phosphate 1-phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015979">
        <property type="term" value="P:photosynthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009749">
        <property type="term" value="P:response to glucose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035966">
        <property type="entry name" value="PKF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43650">
        <property type="entry name" value="PYROPHOSPHATE--FRUCTOSE 6-PHOSPHATE 1-PHOSPHOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43650:SF20">
        <property type="entry name" value="PYROPHOSPHATE--FRUCTOSE 6-PHOSPHATE 1-PHOSPHOTRANSFERASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53784">
        <property type="entry name" value="Phosphofructokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7730262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="203" mass="22402" checksum="88F57AC3587B4BB5" modified="2024-05-29" version="1">MQYAKQKEGTYKGKKFTAICHFLGYQARGSLPSKFDCDYAYVLGHICYHILAAGLNGYMATVTNLKDSLNKWRCGAAPLTLQTNHCYTARPSKVVYIHINTCTVVPLPRVLKQNATKFLMEDVYRNPGPLQFDGPGADSRTVSLHIQDLDYMGRIKELNTYLDKVRSIVKPGCSQDILKAALSAMSSVADVLSVMASPSNGTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BWW9</accession>
    <name>A0AAD5BWW9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">mannose-1-phosphate guanylyltransferase</fullName>
            <ecNumber evidence="3">2.7.7.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_016701</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; GDP-alpha-D-mannose from alpha-D-mannose 1-phosphate (GTP route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.13" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010010496">
        <property type="protein sequence ID" value="KAI7731238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BWW9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004475">
        <property type="term" value="F:mannose-1-phosphate guanylyltransferase (GTP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009298">
        <property type="term" value="P:GDP-mannose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06425">
        <property type="entry name" value="M1P_guanylylT_B_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000013">
        <property type="entry name" value="mannose-1-phosphate guanyltransferase beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056729">
        <property type="entry name" value="GMPPB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045233">
        <property type="entry name" value="GMPPB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050486">
        <property type="entry name" value="Mannose-1P_guanyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22572">
        <property type="entry name" value="SUGAR-1-PHOSPHATE GUANYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25087">
        <property type="entry name" value="GMPPB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleotidyl transferase" evidence="8">
        <location>
            <begin position="2"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="Mannose-1-phosphate guanyltransferase C-terminal" evidence="9">
        <location>
            <begin position="251"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF25087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7731238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="361" mass="39563" checksum="9ADB6CFE42D3A865" modified="2024-05-29" version="1">MKALILVGGFGTRLRPLTLSFPKPLVDFANKPMILHQIEALKATGVTEVVLAISYQPEVMLNFLKDFESKLGIKITCSQETEPLGTAGPLALARDILADETGEPFFVLNSDVISEYPLKEMIAFHKSHGGEASMLVTKVDEPSKYGVVVTEESTGKVEKYVEKPKIYVGNKINAGIYLLNPSVLDRIELKPTSIEKEIFPKMASEKQLYAMVLPGYWMDIGQPKDYITGLRLYLDSLRKKSQSKLASGAHIIGNVLVDESAKIGEGCLIGPDVAIGPGCVIESGVRLSRCTIMRGVRIKEHACISSSIIGWHSTVGRWARVENMTILGQDVHVCDEIYSNGGVVLPHKEIKASILKPEIVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BX36</accession>
    <name>A0AAD5BX36_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ras-related protein RABA6a</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_019977</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Endomembrane system</location>
            <topology evidence="7">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010852">
        <property type="protein sequence ID" value="KAI7730026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BX36"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01868">
        <property type="entry name" value="Rab11_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000274">
        <property type="entry name" value="ras-related protein RABA5a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0636">Prenylation</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7730026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="219" mass="24934" checksum="2A80909820EEDC1E" modified="2024-05-29" version="1">MGSNLFDHDECDYLFKTILVGDSAVGKSNLLSRFSKDEFHLDSKPTIGVEFAYRNMKIGDKIVKAQVWDTAGQERFRAITSSYYRGALGAMLVYDITRRGTFENLRKWLHELREYGSRDMVIILVGNKSDLIDSREVDTEDGQSLAQLEDLCFMETSAKENLNVEDAFLQMITKIFEIASQKSLEAKEKEATKVLHERKEIIYVVDEVSATKQTSCCTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BX61</accession>
    <name>A0AAD5BX61_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MACPF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_026422</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010609">
        <property type="protein sequence ID" value="KAI7730859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BX61"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009626">
        <property type="term" value="P:plant-type hypersensitive response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000031">
        <property type="term" value="P:regulation of salicylic acid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044663">
        <property type="entry name" value="CAD1/NSL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020864">
        <property type="entry name" value="MACPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33199">
        <property type="entry name" value="MACPF DOMAIN-CONTAINING PROTEIN CAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33199:SF1">
        <property type="entry name" value="OS01G0958700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01823">
        <property type="entry name" value="MACPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00457">
        <property type="entry name" value="MACPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51412">
        <property type="entry name" value="MACPF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MACPF" evidence="1">
        <location>
            <begin position="1"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7730859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="652" mass="73699" checksum="D77548D6AB181B18" modified="2024-05-29" version="1">MALKVPAAEAAKVAIESIGCGYDISLDLRLKYRKGYDKNQSFRLIEVDQDEGRDIVLPGGILISNVSKWVKCDKGERTRFSSDVLSFQQMSEQFNQEISLTGKIPSGHFNTMFEFSGSWQKDASSTKTLAFDGVFISLYTVALEKSQMVLCDHVKNAVPSSWEPCQLARFIEKFGTHIIVGVKMGGKDVIYMKQKHASSLQPADVQKKLKEMADKRFLDSDEQYVIDSEHVSPKDKDRASRQHMSILRYSLELVSALESFGLGLRCSSCETRLDFIWLLVFRRYEVREQRLRFADIDSSSSYSYKEDLVSIYKRRGGSDDRNLKHKEWLHSVQLEPDVITMSFVPITSLLNGVSGSGYLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPVFSDLPLGPQRKQQSTASLQFSFFGPKLYVNTNPVDVGKRPVTGLRLYLEGKRSNRLAIHLQHLSSLPKVIQLQDDVDGNFNNEPYDRRYYEKVQWKHFSHVCTAPVESENELSIVTGAHLHVGEYGLKNVLFLRLRFSTALGVAAIKNPEWDGSPGLARKSGLISTLISHHFTAVLKPPPQPADVNINSAVYPGGPPAPVQAPKLLKFVDTTEMTRGPQQTPGYWVVSGARLVVDKGKISLRVKYSLLTLKLPDDEEEDEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BXN1</accession>
    <name>A0AAD5BXN1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mei2-like C-terminal RNA recognition motif domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_025096</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010796">
        <property type="protein sequence ID" value="KAI7730339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BXN1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007201">
        <property type="entry name" value="Mei2-like_Rrm_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23189">
        <property type="entry name" value="RNA RECOGNITION MOTIF-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04059">
        <property type="entry name" value="RRM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Mei2-like C-terminal RNA recognition motif" evidence="3">
        <location>
            <begin position="283"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="75"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="81"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7730339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="380" mass="41090" checksum="0DB8F0B679D9CAAE" modified="2024-05-29" version="1" fragment="single">PVEHNPLQNVSKSPVLGSVSPNFGNGLSGLASILHPQPARIAPIGKDNGRTNRNHLEQNNTFQQSHSLPNSILSQFNEPVSSFGGSSSTGSGIETLSGPQFLWGSPNPSPSILTDQPKGPSHGFRSPTVGHPFTTSKVPPGITHGFPITGRHGSLLHQHHHVGSAPSGIPFEGHFGHYHESSETVFMSPPAYGGVGVGHIDRGFLGSRGSIGNGSPSFSTMHSPRVSSMFLGGGPYAGFGATIAENMSDRGRNRRADQNGSQTDSKKQFQLDLDKITSGEDTRTTLMIKNIPNKYTSKMLLAAIDENHSGTYDFLYLPIDFKNKCNVGYAFINMLSPTHIVPFYQVFSTLRRSMGKNGRNSTVKKLLPWLMLESKGRWHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BY97</accession>
    <name>A0AAD5BY97_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_022445</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010401">
        <property type="protein sequence ID" value="KAI7731692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BY97"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03213">
        <property type="entry name" value="ABCG_EPDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000337">
        <property type="entry name" value="ABC transporter G family member 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050352">
        <property type="entry name" value="ABCG_transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041:SF22">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="346"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="378"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="419"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="455"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="485"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="562"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="5"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="590"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7731692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="590" mass="65818" checksum="0EEC97C33546CB2D" modified="2024-05-29" version="1" fragment="single">FQEVVYKIDIEKQGCIKNNNNIDNPKEKQILKGVTGMVLPGEMLAMLGPSGSGKTTLVTALGGRLGGKLGGTITYNGKPFSSIIKRNTGFVTQDDVLYPHLTVTETLVFTALLRLPNSLTNQQKIAKAEDVIDQLGLIRCKTSIIGGPTLRGISGGERKRVSIGQEMLINPSLLFLDEPTSGLDSTTAQRIVSTLWELAEGGRTIVMTIHQPSSRLFYMFHKVLLLSDGNPLYFGKGSEAMSYFHSIGFSPFVAMNPSDFLLDLANGISPDDSNPEDQGTIKQKLVTAYKLKLDDKLKAETREYEEQVDDVLDYKKSEQWNTTWLQQFTILLKRDLKERRHESFDVLKIAQVLVISVLCGLLWWQSDTAHIQDQSGLLFFYTGFWGFFPLFQAIFTFPQERAMLSKERSSGMYRLSSYFMSRTIGDLPMELVLPTFFCIVTYWMAGLKPNLGSFLYALFALLLSVLVSQGLGLALGALVMDLKSATILGSVIMLSFTLAGGYYVQHVPVFISWIKYVSISLYTYKLLLGSQYEHGQTYRCGNQTCLVADFPAIKNIGLDGQFTSFIALVIMLVFYRIVAYIALMRIGVPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BYG8</accession>
    <name>A0AAD5BYG8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_011361</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010317">
        <property type="protein sequence ID" value="KAI7732063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BYG8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08241">
        <property type="entry name" value="QOR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002364">
        <property type="entry name" value="Quin_OxRdtase/zeta-crystal_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051397">
        <property type="entry name" value="Zn-ADH-like_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43677:SF4">
        <property type="entry name" value="QUINONE OXIDOREDUCTASE-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43677">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE/REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01162">
        <property type="entry name" value="QOR_ZETA_CRYSTAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="2">
        <location>
            <begin position="45"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7732063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="371" mass="40142" checksum="9AB0A67BFBFAE78C" modified="2024-05-29" version="1" fragment="single">REREVFREREREPETMETTGDDGGLPETTTQKLGDPTTPPDSSPTTALTISTSHPIPTLTSPTSIRVQIKSTSLNYANYLQILGKYQEKFPLPFIPGSDYSGVVESVGPNVTKFKVGDRVCSVANTGTFARFIVVDEKDLYRVPDGCDLVAAGALPVAYGTTHVALVHRANLKSGQVLLVLGAAGGVGLAAIQIGKICGATVVAAARGTEKVEFLKSMGVDHVVDLSKEGVIDSVRAFLKAKKLKGVDVLYDPVGGKLTKESMKLLNWGAQILVIGFASGEVPLIPVNIALVKNWTIHGLYWGSYKYNNPSVLEDSLKELLSWLARGLIAINISRSYKLQEANLAFSDIRDRKVIGKVMITFDDPRTISKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BYQ8</accession>
    <name>A0AAD5BYQ8_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_008944</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Co-chaperone that regulates diverse cellular pathways, such as programmed cell death and stress responses.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010350">
        <property type="protein sequence ID" value="KAI7731932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BYQ8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000774">
        <property type="term" value="F:adenyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17054">
        <property type="entry name" value="Ubl_AtBAG1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.120:FF:000011">
        <property type="entry name" value="BAG family molecular chaperone regulator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000298">
        <property type="entry name" value="BAG family molecular chaperone regulator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.120">
        <property type="entry name" value="BAG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039773">
        <property type="entry name" value="BAG_chaperone_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036533">
        <property type="entry name" value="BAG_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003103">
        <property type="entry name" value="BAG_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12329:SF11">
        <property type="entry name" value="BAG FAMILY MOLECULAR CHAPERONE REGULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12329">
        <property type="entry name" value="BCL2-ASSOCIATED ATHANOGENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02179">
        <property type="entry name" value="BAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00264">
        <property type="entry name" value="BAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63491">
        <property type="entry name" value="BAG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51035">
        <property type="entry name" value="BAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="4">
        <location>
            <begin position="45"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="BAG" evidence="5">
        <location>
            <begin position="140"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="228"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="31"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="249"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00058673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7731932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="275" mass="31156" checksum="D3AB2E844292A848" modified="2024-05-29" version="1" fragment="single">MMHMKTKSTEPPSVNGGDKEWEVRPGGMLVQRRDPTEEQNRLPPPTIRVRVKYGSVYHEINISSQATFGELKKMLSGPTGLHHEDQKLMYKDRERSSKTFLDVVGVKDRSKMVLVEDPISQEKRYIEMRKNAKMEKAAKSISEISLEVDRLAGQVSAFESVISKGGKVAEKTLLNLIELLMNQLLKLDGITAEGDVKLQRKMQVKRVQKYVETLDVLKIKNSIIGTNENGDEKQVKEPPASQGQEERFSSNGHVNAASPNQHERYQTPSFPKSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BZ35</accession>
    <name>A0AAD5BZ35_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">MPN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_024483</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">May be part of a complex that binds ubiquitinated proteins and that is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Protein degradation; proteasomal ubiquitin-dependent pathway.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NPL4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010243">
        <property type="protein sequence ID" value="KAI7732408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BZ35"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08061">
        <property type="entry name" value="MPN_NPL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17055">
        <property type="entry name" value="Ubl_AtNPL4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000331">
        <property type="entry name" value="NPL4-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016563">
        <property type="entry name" value="Npl4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007717">
        <property type="entry name" value="NPL4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024682">
        <property type="entry name" value="Npl4_Ub-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12710">
        <property type="entry name" value="NUCLEAR PROTEIN LOCALIZATION 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12710:SF0">
        <property type="entry name" value="NUCLEAR PROTEIN LOCALIZATION PROTEIN 4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05021">
        <property type="entry name" value="NPL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11543">
        <property type="entry name" value="UN_NPL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="MPN" evidence="6">
        <location>
            <begin position="130"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00060043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00060618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7732408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="413" mass="46244" checksum="C2A637220683E067" modified="2024-05-29" version="1">MTLIRIRSRDGIERLQINNPNATIADLKTLIEQHLHVPINNQTLSTDKHLLLAKTPNEIARFNDMSNPDTLIQTLGIVHGSLIFLAYEGERTIAGPKVVPAGSFGRKMTMDDLIAKQMRIVRQENAKCEMVSFDRDAANGFQNYVNESLGFGVKRGGFMYGTVSDEGKVEVDFIYEPPQQGTEDSLVLMRDENEERVVEAIALGLGMRKVGFMFTQTVGQQGKREYTLSNREVLQAVEMQGESELKEWVTVVVKLEVNEEGGADVHFEAFQMSDLCVKLFKEGWFETEVGEGDDPKLSKMKKDVVVGGKDTREVDNDFFLVVVKIFDHQGPLSSTFPVENRNTVITMRTLKNHLDRTKNLPFVKRISDFHLLLLLGRFLDVNSDVPALAGCVRAQATIPEGYQILIESMAASG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C0N9</accession>
    <name>A0AAD5C0N9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_001897</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010175">
        <property type="protein sequence ID" value="KAI7732719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C0N9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="293"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="351"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="293"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="307"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7732719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="439" mass="48451" checksum="39E89E5FC7DE8591" modified="2024-05-29" version="1">MTDLPSGEISSEPAGHAVPGASTNLTRLVVFRNGDPIILRDSEPESDNVDPNHQNIQDLELGADLNYQNTQDMIADNITDAEEKHNRLKRGRKRKMDSLTLEAVEKFFGKPIEEAAKSLDGNRSTLKRFCPVHDMTCQVDMPSGEISSEPSGHAVPSKSTDLTPLVVFRRDSQPESDNVDPNHQNIQDLELGADLNYQNTQDKIAQQLTSLPGLHDPGLSTENWPSAQQYESDDALWELLWADSMIQKSDFSASNVSFGSRENNKVISHLNPMGAALIIISGVVMDHEKSCQGKMDHGLNESEPRKSGSFSHISSTNSSFRRRSFNRFGSSHHEIDDDSVSEAGDIGDRALSSRRYSESGRPRFSFDNTGENVGLPIQEHPFKDSQIPTASPASPGAILLGKDQNEECKKELPWYLTYMSSRVHLAVLGILGLRTALSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C141</accession>
    <name>A0AAD5C141_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_031360</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010316">
        <property type="protein sequence ID" value="KAI7732081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C141"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.420.10">
        <property type="entry name" value="Peroxidase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7732081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="147" mass="16375" checksum="1FF01C6436693FB3" modified="2024-05-29" version="1">MCSPEWIDTWWHDIYVWFELGSMDVPVRGVFTAPHDTFGASEGLMMPPFIPPQSQVAVPKDGPGAPGQSWTVKWLRFDNSYFKVYAEKYVVDEKAFFDDCAEVHAKLSNLGAKFDPPEGFSINDEPPPPAVAAPEKFVAAKYSSGKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C163</accession>
    <name>A0AAD5C163_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_033407</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010094">
        <property type="protein sequence ID" value="KAI7733050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C163"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7733050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="60" mass="7060" checksum="D2F9CC94C06F697E" modified="2024-05-29" version="1" fragment="single">GKRPQKFLESQLKPNQLKEYGHSYLGNERFTSYMALIRYKGALMQAPLRPHTNQQLQQLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C571</accession>
    <name>A0AAD5C571_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Patatin</fullName>
            <ecNumber evidence="7">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_030829</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Lipolytic acyl hydrolase (LAH).</text>
    </comment>
    <comment type="domain">
        <text evidence="7">The nitrogen atoms of the two glycine residues in the GGXR motif define the oxyanion hole, and stabilize the oxyanion that forms during the nucleophilic attack by the catalytic serine during substrate cleavage.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the patatin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="7"/>
    <dbReference type="EMBL" id="JAMZMK010009524">
        <property type="protein sequence ID" value="KAI7735255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C571"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047372">
        <property type="term" value="F:monoacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004620">
        <property type="term" value="F:phospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07214">
        <property type="entry name" value="Pat17_isozyme_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1090.10:FF:000005">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32176">
        <property type="entry name" value="XYLOSE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32176:SF92">
        <property type="entry name" value="XYLOSE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 6 7" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="5 6 7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="3" id="KW-0611">Plant defense</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PNPLA" evidence="8">
        <location>
            <begin position="25"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXGXXG" evidence="6">
        <location>
            <begin position="29"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="6">
        <location>
            <begin position="67"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DGA/G" evidence="6">
        <location>
            <begin position="213"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="69"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7735255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="404" mass="43759" checksum="3E80875D49C8688E" modified="2024-05-29" version="1">MTSETTSTSRIGLPPPTSGNLITILNIDGGGIRGIIPGVILQYLESELQALDGEEARLADYFDVVSGTSTGGLITVMLTAPNENNRPLYAAKDIVPFYVENSPQIFPQVGGPFAGFIKLLKTLVGPKYNGKYLKNLLTTLLGSTKLSQTLTNVVIPTFDIRDMQPVIFSSFQVPREPSMDAQLSDICIGTSAAPTYLPAHYFQNGDREFNLIDGGVAANNPSLVAIGEVTRQVLKSDPNFPTIKPLEYDRYLLISIGTGMQKQTPKFDAKMAAKWGVIGWLTNQGSTPLIEAFTQASADLVVMHNNVIFETLDSINNYLRIQDDDLTGDLASVDVATKENLDGLVEVGKALLDKPVSRVNTDTGVVEPVPDGGTNREALKRFAERLSNERKLRESKYSDGGVTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C5X7</accession>
    <name>A0AAD5C5X7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_022358</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009398">
        <property type="protein sequence ID" value="KAI7735946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C5X7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7735946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="136" mass="14446" checksum="644B92E3E514FBF1" modified="2024-05-29" version="1">MGMGLIPTGDIGSVVTGASEVTILFSSGPGRASLALRHIDFIFQKICTWSILFIQKFNGFSCTVLVTMSTDPACTAWAKVGAILVVVGEVLLVDDAWLLSGHHYRNCLPHVLSAVVDLGGNFAASAMKAELHTLQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C9C1</accession>
    <name>A0AAD5C9C1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">FAD-binding PCMH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_007429</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oxygen-dependent FAD-linked oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009113">
        <property type="protein sequence ID" value="KAI7737120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C9C1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.462.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.10">
        <property type="entry name" value="Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012951">
        <property type="entry name" value="BBE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016167">
        <property type="entry name" value="FAD-bd_PCMH_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32448">
        <property type="entry name" value="OS08G0158400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08031">
        <property type="entry name" value="BBE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="7">
        <location>
            <begin position="1"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7737120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="455" mass="51470" checksum="D053F709EA6CA2A1" modified="2024-05-29" version="1">MPQPQILRFNIQIRIRSGGHDNAGLSYTSTYQSSFVVLDLRELRSVTIESGDHNTAWVETGATLGELYYRVSQESPNLGFAAGTCPTVGVGGHISGGGFGNMMRKYGLAADNVIDARIVDANGKILDRKSMGEDLFWAIRGGGGGSFGVVVAWKLKLVYVPDKVSVFSLSKTIEEGGSSLFNKWQYISHKVSRDLFMRVIALPALNEAGNRTIQVIFNSMFLGNVDKLMETITDSFPELGLQEKDCSEMSWIETVLYFDNYTVGRSIDVLKDRKPVPKSYFNGKSDYVKEPIPKEKLEEIWKWCLEGDSPVLVMEPHGGMMHEIDDTRIAYPHRKGYLYNIQYLEQWQDDSTESSAKRISWMRKMYENMTPYVAKYPRAAYVNYRDLDLGENDNADNTSYLNAIEWGSKYFGDNFRRLAMVKGVVDPDNYFFYEQSIPPLIVSEEKCGIGSYKSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C9V0</accession>
    <name>A0AAD5C9V0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_022834</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008916">
        <property type="protein sequence ID" value="KAI7737988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C9V0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044296">
        <property type="entry name" value="HIPP46"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46371">
        <property type="entry name" value="OS04G0464100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7737988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="126" mass="13327" checksum="116D52D5A9D909E4" modified="2024-05-29" version="1">MAKQKIVVKMAANNEKKSRKALKIAVSVPGVESVAFVGSDKDQIALTGEGIDSVELATLLRKSVGYTELVSVGSVEEKKPAAPASSVAVQTAAVPPQLVYPHHYGYGVPYHVYEGPCHNDSSCTVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CA97</accession>
    <name>A0AAD5CA97_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">WRKY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_011241</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the WRKY group I family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009192">
        <property type="protein sequence ID" value="KAI7736806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CA97"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.80:FF:000006">
        <property type="entry name" value="WRKY transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.80:FF:000003">
        <property type="entry name" value="WRKY transcription factor 57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.80">
        <property type="entry name" value="WRKY domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003657">
        <property type="entry name" value="WRKY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036576">
        <property type="entry name" value="WRKY_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044810">
        <property type="entry name" value="WRKY_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31221:SF90">
        <property type="entry name" value="WRKY TRANSCRIPTION FACTOR 44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31221">
        <property type="entry name" value="WRKY TRANSCRIPTION FACTOR PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03106">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00774">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118290">
        <property type="entry name" value="WRKY DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50811">
        <property type="entry name" value="WRKY"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="WRKY" evidence="11">
        <location>
            <begin position="154"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="WRKY" evidence="11">
        <location>
            <begin position="311"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="115"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="115"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00061157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7736806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="381" mass="42739" checksum="CC5F2C934BEAD39E" modified="2024-05-29" version="1">MNIEEAAERTVIAKPVASRPTISDFRSFSCLIASSEPTVTAIRPKTVRFKPAMSSAPPEMVSSQAELTGTRACHSSDKILKSNHTSPLLYKPLAKTVSRTAISLLANMETCNASHEQALTQNPASQSETVEGTNLPKPMPVKSGNDGRPQLYANNSDGCYDGYNWRKYGQKQVKGSEYPRSYYKCTHPNCMVKKKVERSVDGKIAEIVYQGEHNHSKPRVPRRHAFDATSEDLNRQFPNNQVNDDEVLGSSSRVTNSVKVKESCNHGSNEAFDVPRVQDDLMSKRRKSFSRLNETQGEGLQEPRVVVHDGTDSEAISDGFRWRKYGQKIVKGNPYPRSYYRCTSVKCNVRKHVERASDDPNVFITTYEGKHNHEMPIKKPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CAN4</accession>
    <name>A0AAD5CAN4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Expansin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_005414</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Causes loosening and extension of plant cell walls by disrupting non-covalent bonding between cellulose microfibrils and matrix glucans. No enzymatic activity has been found.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Secreted</location>
            <location evidence="7">Cell wall</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Membrane</location>
            <topology evidence="7">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the expansin family. Expansin A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009150">
        <property type="protein sequence ID" value="KAI7737001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CAN4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009664">
        <property type="term" value="P:plant-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22274">
        <property type="entry name" value="DPBB_EXPA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.760:FF:000001">
        <property type="entry name" value="Expansin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.760">
        <property type="entry name" value="Expansin, cellulose-binding-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="entry name" value="RlpA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007118">
        <property type="entry name" value="Expan_Lol_pI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002963">
        <property type="entry name" value="Expansin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007112">
        <property type="entry name" value="Expansin/allergen_DPBB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007117">
        <property type="entry name" value="Expansin_CBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036749">
        <property type="entry name" value="Expansin_CBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009009">
        <property type="entry name" value="RlpA-like_DPBB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31867">
        <property type="entry name" value="EXPANSIN-A15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03330">
        <property type="entry name" value="DPBB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01357">
        <property type="entry name" value="Expansin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01226">
        <property type="entry name" value="EXPANSIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01225">
        <property type="entry name" value="EXPANSNFAMLY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00837">
        <property type="entry name" value="DPBB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="Barwin-like endoglucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49590">
        <property type="entry name" value="PHL pollen allergen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50843">
        <property type="entry name" value="EXPANSIN_CBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50842">
        <property type="entry name" value="EXPANSIN_EG45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0134">Cell wall</keyword>
    <keyword evidence="6 7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Expansin-like EG45" evidence="8">
        <location>
            <begin position="2"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="Expansin-like CBD" evidence="9">
        <location>
            <begin position="124"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7737001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="207" mass="22376" checksum="EFCF66A6FCC90B6D" modified="2024-05-29" version="1">MGGACGYGNLYSQGYGTNTAALSTALFNNGLACGACFEIKCVNDKRWCLPGSIIVTATNFCPPNPAQSNNDGGWCNPPLQHFDLAQPVFLKIGQYKAGIIPVQYRRVACKKRGGVRFTINGHSYFNLVLLTNVGGAGDVIAVSIKGSRSRTWQAMSRNWGQNWQSGAYLDGQALSFKVTTSDGRTVVSNNVTPPNWSFGQTYTGRQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CAZ4</accession>
    <name>A0AAD5CAZ4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_000216</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008802">
        <property type="protein sequence ID" value="KAI7738512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CAZ4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056511">
        <property type="entry name" value="IDM1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032308">
        <property type="entry name" value="TDBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46508:SF2">
        <property type="entry name" value="INCREASED DNA METHYLATION 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46508">
        <property type="entry name" value="PHD FINGER FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23209">
        <property type="entry name" value="IDM1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16135">
        <property type="entry name" value="TDBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="8">
        <location>
            <begin position="505"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetyltransferase" evidence="9">
        <location>
            <begin position="656"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="296"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="345"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="309"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="350"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7738512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="862" mass="96834" checksum="50374ED4F1145D0F" modified="2024-05-29" version="1">MESHVSKLLVIRPEPIQCSKRKWKDSSFVELDEDELLALPEDSATDIKLLLRFHIHRLLREAKWVIGRRNRTTHCKGHGEFVFKSPSGRPIREFYRAWNMCGQKLVADANYVAGDSNGIQWTSLPEFQSDFSDTLIEIEEQLRNLEPATALAHWWYLLDPFAKVVFIDKSLRNLKEGKPVKTKRSVVDGFYFSDDPVLPSTNEVIVANQITKKVGKGSSDLVPFSCQTNNLYSSTGGSEIVFAHEDGVMQTKEHKKSKKLSEMKLSASYGMQEVSEISVQLKSNVAESNGSYLKSSFCSSDDQHEKKQSRLKKSTHHHKHGKEKKGHCNLSDDDLLLSAILKNRSTNKSSRVKTKSRVPKATRRSNKGTKGSCRLRPRSLTKGDQHQVEGRWSGLGVRTVLSWLIDFGVIKVNEVIQYRNPRDDLVVKDGLVTRDGILCQCCEKILSVSEFKSHAGFSLKSPCLNLYMESGKSYTLCQLEAWSAEYKVRKGATRTIEVEDIDEHDDSCGLCGDGGELICCDNCPSTFHLKCLCVEELPEGNWYCSRCSCWICGNVVDDNEASSLGALKCLQCEHKYHVECLREKGMERELGPSASWFCGESCEEIHSGLHSQIGIMNPISDGFSCTLLKCIHGDLKVHSAQRLVALKAECNLKLAVALTIMEECFLPMVDPRTGIDMIPHVMYNWGSEFARLNYEGFYTIILEKDDVIVCVASIRIHGANVAEMPLIATCSRYRRQGMCRRLMNAIEEMLKSLKVGKLVVSAIPSLVDTWTEGFGFTHLEANEKKSLTKTNLMVFPGTVWLTKPMYQSAVQSPPTEAEDLGEDEDQDLMLSREEPPQASLKAEVNSFDTGFEVNPIITGNFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CBE4</accession>
    <name>A0AAD5CBE4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_000886</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008792">
        <property type="protein sequence ID" value="KAI7738577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CBE4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="182"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="182"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7738577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="428" mass="45192" checksum="28A3492CC21DE4A9" modified="2024-05-29" version="1">MVIGTRSCKRQHVELSPAGALVCLPYDVQANTIDVGSSTPVLKAIKKLKRTLKSTQTPRETMSVTARRRPPNGKDGCVGENIELALRVAREKLAEFKLVKLGVEKILSSLSSKHPNNMRVVALTDDYKKSLSFAREDAGETSKTLDTPSKRMMDVVPELGECYGIVAMSNVAVDVQEGHVESADVDSHEIPLGRDASGDDDVLSDSSEDNDWLFTRVGAVGQGSGGHPENDPTVGASGVPVTGILSNTSAGVIKQSDPIQGDTASNISPSKNVAMNIGVETTKNESAVPTSLGLGFKQALVHVVQWCGEMQSTLAGAMGAHRKKLGLACTIWKPDAGVKDDQTTAPSQSSFTLGLTQDIVPNCGAGAVGCVEDQFTSRAGKKYNEGMAGDLVVNMPNDVAGEKLSGDQMLRIMVRGALLATKILSPHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CDD2</accession>
    <name>A0AAD5CDD2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DOMON domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_021526</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008818">
        <property type="protein sequence ID" value="KAI7738396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CDD2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd09629">
        <property type="entry name" value="DOMON_CIL1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045265">
        <property type="entry name" value="AIR12_DOMON"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005018">
        <property type="entry name" value="DOMON_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23130:SF157">
        <property type="entry name" value="AUXIN-INDUCED IN ROOT CULTURES PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23130">
        <property type="entry name" value="CYTOCHROME B561 AND DOMON DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04526">
        <property type="entry name" value="DUF568"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50836">
        <property type="entry name" value="DOMON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041953342" description="DOMON domain-containing protein" evidence="6">
        <location>
            <begin position="31"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="DOMON" evidence="7">
        <location>
            <begin position="52"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7738396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="241" mass="25623" checksum="83A3E0302022434D" modified="2024-05-29" version="1">MSFNHPNPPSLRLLLLTCASLLSLTSPAHSITCSSQKFSNNLYSNCTDLPTLNASLYWNHINSSSFSIAFVAPPSKPDGWIAWAINPTGTGMAGSQALIAFKDSKGMMNVKTYNISSYSSIVEGKISFEVSGSRAEYSAGSMKIYATVKLPETMTEVNHVWQVGGSVVDGVPAKHEFQPDNLNAKGKLMLRVAKKAHVSGGPVGSPAGAPSPNPNPVPSSGYRNNVMYVLFMVIVGALISV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CET1</accession>
    <name>A0AAD5CET1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_024117</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008403">
        <property type="protein sequence ID" value="KAI7740566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CET1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7740566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="116" mass="13048" checksum="66EEEB04B2657E89" modified="2024-05-29" version="1">MRDVKVTCPRYCCRRKDFLEVSHAIFHTYFFSTQTNANCEATTNSGSSGQMNIDPCAWTSEGYRTPFLAQVIIKHAKAGVLDDKVYVYSCVDGIGLLFNSILEVVVQPLMVKIIYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CFG7</accession>
    <name>A0AAD5CFG7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_013895</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008385">
        <property type="protein sequence ID" value="KAI7740647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CFG7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35719">
        <property type="entry name" value="OS01G0680600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35719:SF5">
        <property type="entry name" value="T6K12.7 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="152"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="199"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="204"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="225"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="237"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7740647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="269" mass="30811" checksum="A2E4D7EBA93FAD91" modified="2024-05-29" version="1" fragment="single">MVFTVFFIRIHPPPPPLLPLHHRQPPWPMVSPAASFFINPSRIIIPLRLQANRRWDSNAETFRTTNFDFDSIDDDDFEDDDDDDSSQWLDILEDFIDGVWIFKVFRSFGWMLPAIISSLLLTSGPKAFLMALAIPMGQSALSLLFQTVWGRPRAKTRRRGKSKRKQPPPRQPPRGASYMDIDDEEEYVEGERMRATGYQTWVAGDGSSSDKTNGSSSSFGGWEELDGRTRTRSSKDGKRKQSKSKMSRRVKRSETPLLLRLLIAVFPFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CH32</accession>
    <name>A0AAD5CH32_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_000834</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008207">
        <property type="protein sequence ID" value="KAI7741374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CH32"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016702">
        <property type="term" value="F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.640.10">
        <property type="entry name" value="Haem peroxidase domain superfamily, animal type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037120">
        <property type="entry name" value="Haem_peroxidase_sf_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050783">
        <property type="entry name" value="Oxylipin_biosynth_metab"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11903:SF31">
        <property type="entry name" value="PEROXIDASE SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11903">
        <property type="entry name" value="PROSTAGLANDIN G/H SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7741374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="122" mass="14089" checksum="691F852DD15108B6" modified="2024-05-29" version="1">MKSFMSFAKNPLLSPIKHFIHPDFHKFYEKMRPIDRPLFLIVHGVDKSRIGWHRFPVFLGLTYLGIRRNLHDKYNLLNVGNTPVGDRYDNLKEGASSEGTFFGRNMVPVDKKDKVSIGISTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CII5</accession>
    <name>A0AAD5CII5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HMG box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_007633</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007977">
        <property type="protein sequence ID" value="KAI7742472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CII5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044601">
        <property type="entry name" value="HMGB6/HMGB13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46912">
        <property type="entry name" value="HIGH MOBILITY GROUP B PROTEIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46912:SF1">
        <property type="entry name" value="HIGH MOBILITY GROUP B PROTEIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMG box" evidence="3">
        <location>
            <begin position="45"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="HMG box" evidence="3">
        <location>
            <begin position="171"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="1">
        <location>
            <begin position="45"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="1">
        <location>
            <begin position="171"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="25"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="148"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="153"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="183"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7742472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="254" mass="30092" checksum="441AAA39BA5CC129" modified="2024-05-29" version="1">MKLLEDEQKQKMAMELLEQYLQFKQEAEKDGDNKKNKKEKDPLKPKRPESAYFLFMNERRAALIAENKSVVEIAKITGEEWKNMTEKQKARYAKVANEKNEKYTQEMEIYKQNKETEAEIAKKEEDEVLKVLKQEALQLLKKKEKTETIIKKTKEKRNKTKNNKNDDPNKPKRPASSFLLFSKETRKDLSKEKPDISNAQLTALISVKWKELGEEEKQRWNSEAAKAMEIFKKEMEEYNKKNVVETPKNDDNQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CIJ1</accession>
    <name>A0AAD5CIJ1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_001132</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008250">
        <property type="protein sequence ID" value="KAI7741186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CIJ1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7741186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="81" mass="9283" checksum="253DFFC482E6441D" modified="2024-05-29" version="1">MLQWNLTKGCITGQPLPRLLVFGLKVMTILFYTKEVLLYTVGLNIPNIVSHTIVVTIHYLIRYSFPMVSLAGILIYHVTEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CJA6</accession>
    <name>A0AAD5CJA6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_005436</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008110">
        <property type="protein sequence ID" value="KAI7741890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CJA6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd20071">
        <property type="entry name" value="SET_SMYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044237">
        <property type="entry name" value="ATXR2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002893">
        <property type="entry name" value="Znf_MYND"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47436">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE ATXR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47436:SF1">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01753">
        <property type="entry name" value="zf-MYND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144232">
        <property type="entry name" value="HIT/MYND zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SET" evidence="4">
        <location>
            <begin position="38"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7741890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="432" mass="48977" checksum="2BDAC713BA74CD9E" modified="2024-05-29" version="1">MEVVDAQFSHQISLLLEPPSPLQVKEYFHELTTTKHLHGIQVKPTNHYGKGVYADIDFEEEELVLKDQMLVGMQHSSNKIDCLVCGCCFRFIGSIEIQIGRKLYLQGLGGGPHDDCFNEKVELLMHQRLELPHSKKFPLPEVINCPGGCKEVYYCSKYCAQADWDSCHSLLCTGDGSKSISNKALSKFIQHANETNDIFILAAKAISCTILKYRKLKAACDKENQKEKNHLSDVLGNHNFSLLLKAWKPLSMGYKRRWWDCIALPDDVDVSDEASFRMQIRQLAFESLELLKAAILDEECLPSSPVEDYFLYVDDLPEAEKKEVDEITQPFLDALGEDYSVCCQGTAFYPIQSCMNHSCCPNAKAFKREEIIIKQREDKDGQATILACKPIKKGEEITISYIDEDLDLEERQALLADYGFICKCARCVEEDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CNG5</accession>
    <name>A0AAD5CNG5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_029842</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007429">
        <property type="protein sequence ID" value="KAI7744847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CNG5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009658">
        <property type="term" value="P:chloroplast organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45641:SF19">
        <property type="entry name" value="NEPHROCYSTIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45641">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN (AFU_ORTHOLOGUE AFUA_6G03870)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="915"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="205"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="947"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7744847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="947" mass="105848" checksum="2B5D81FF4B786B39" modified="2024-05-29" version="1" fragment="single">SKQVEKQIQAHPLTTSQFTTTTDDNRHTPPMSSLCPSIRSDLLKKGIVSFFNQPQKGKCNTRLYVVPNGISTRCSVFRCYATAKSLQVGGPVNVNQVSSVDNGSEKSQRKQLGDDLEMMLEELFSEIKGLIKMGNKGDALDLLEANYEAVKEQIIAGDKTIQEAAVLDVIALGYMAIGDLKMVKPILSTLDEIVRDLGDDEPLVDSILIHMGSMHSTLGRFEKSMLFYKRALEISERNHGDNSASLVMPLLGMGKALGCVGRATKAIDIYNRIISILESNLGAESEEIVVPLNALGNLLIEQGKAQDAENPFMRIVGIYTKIYGKNDQRVGMAMCSLANAKCAKGDAESAIKLYKNALQVLEHSENMNLDDSILEKTRIDLAELLHVVGREEEGRKLLGECLSITKKYKGEEDPSFVTHVINLATSYSRSKNYVEAERLLRSSLEIMERTVGLDDPSITFVMLQLAVTLYNMNRDEEAEQLALKVLRVREKAFGDASVPVGEALDCLICIQKRMERDDGEILDLLKRNLSIQEKAFGDESEQVVETLKKIVFFMDRLGIKDHKAPFQRRLSLLRNKIMRFRFEASNTHKFVCKQIKSCHKTMPFCEVGDYQSGTDAINNKGIKIFYKTYGGGPIKVFMIIGLAATHDSWNPQIEGLVGSVKPNDVVDRVSDKDSDNERGGMIASKLAALFPERLLSLALLNVTGGGYECLPKLDRRTLSIAVRFLRAKTPEQRAAVDLDTHYTEEYLAEHVGVETRRAVLYKEYVKAISSSGMQSNHGFDGQIHACWTHKISRTDIEMIRNEGFLISVIHGRYDVIAQVSHARRLAKKLYPYAKMVELHGGHLVSHERTKEVNEALLELIRAAETKTSPHDWTNLSEKSTGFWSMNWNCSSAYNSEGSGSSESINISIAETIRRMMVYIFSLFVLAFGFIRRVPRRIRPVRVGTGFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CPR9</accession>
    <name>A0AAD5CPR9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_002251</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. KIN-13 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007373">
        <property type="protein sequence ID" value="KAI7745050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CPR9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007019">
        <property type="term" value="P:microtubule depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903338">
        <property type="term" value="P:regulation of cell wall organization or biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01367">
        <property type="entry name" value="KISc_KIF2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000012">
        <property type="entry name" value="Kinesin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47971:SF9">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIN-13B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47971">
        <property type="entry name" value="KINESIN-RELATED PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 7" id="KW-0493">Microtubule</keyword>
    <keyword evidence="4 6 7" id="KW-0505">Motor protein</keyword>
    <keyword evidence="2 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="9">
        <location>
            <begin position="199"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="75"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="553"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="106"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="566"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="585"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="290"/>
            <end position="297"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00061030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7745050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="663" mass="73212" checksum="DB01E6BA959CCD82" modified="2024-05-29" version="1">MNAVGRHGQRSSASGPHHQRQYSDNFLEATSSSNNNNNSRWLQSAGLQHLQQPSSNASSDFGYYYGGGGVGGQGGSRMNVGAQRSLSGGTDLFAEPLTPPHQKKNGGNGGGGGGGGDDNNGFSPGLLDLHSFDTELISEVPMTGAYGAPPYNGDRGRGFDDAELYLGNNKQASKARGLPDNNLVKSFAPEKEKTSNVAKIKVVVRKRPLNKKELAKNEEDIITTEPHSNCLTVHETKLKVDLTEYMEKHGFVFDAVLNEEVSNDEVYHETVEPIVPIIFQRTKATCFAYGQTGSGKTFTMKPLPLKASRDILRLVNHTYRSQGFQLYFSFFEIYGGKLYDLLNDRKKLCMREDGSKQVCIVGLQEYKVSDVETIKELIERGNATRSTGTTGANEESSRSHAILQLVVKRSVDGSETKPPRVVGKLSFIDLAGSERGADTTDNDKQTRMEGAEINKSLLALKECIRALDNDQGHIPFRGSKLTEVLRDSFVGNSRTVMISCISPNAGSCEHTLNTLRYADRVKSLSKGNKKDATFSSKEPTSVSMSTTVLPRVSSYEDNMVDSYPEQVDEDEYESAEEFYEAEQPSWKNTNSKVEPYSYSNAPDKNRRTNGQTTSREPPKSETRNWNPDDELNALLQEEEDLVNAHRKQVEDTMDIVRVISKAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CPV7</accession>
    <name>A0AAD5CPV7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_021071</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007135">
        <property type="protein sequence ID" value="KAI7745787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CPV7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7745787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="68" mass="7868" checksum="9666B4077A93A92E" modified="2024-05-29" version="1" fragment="single">EHKRLSWKQKNMGNGGANITKRIEDGAKEEGGGTNEKEMIMFKMIIQMLCIMVVQAQQKEFKMMVHKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CQP9</accession>
    <name>A0AAD5CQP9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">LOV domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_019595</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007231">
        <property type="protein sequence ID" value="KAI7745485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CQP9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009881">
        <property type="term" value="F:photoreceptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009637">
        <property type="term" value="P:response to blue light"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000036">
        <property type="entry name" value="Putative LOV domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001610">
        <property type="entry name" value="PAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47429:SF8">
        <property type="entry name" value="PHOTOTROPIN-1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47429">
        <property type="entry name" value="PROTEIN TWIN LOV 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13426">
        <property type="entry name" value="PAS_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00086">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0157">Chromophore</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0288">FMN</keyword>
    <keyword evidence="1" id="KW-0600">Photoreceptor protein</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0716">Sensory transduction</keyword>
    <feature type="domain" description="PAS" evidence="8">
        <location>
            <begin position="93"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="9">
        <location>
            <begin position="167"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="71"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7745485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="248" mass="27735" checksum="F93DBB9510BFF7FC" modified="2024-05-29" version="1">MDMTDAESSTKKTNNKWMAFDKDPVQNKPPVARVTPPEDGEIDMETITERTAEWPGGAMVASLIGDEEMAKRSSSASWRSSHDDSFSSNQPRVSQELKDALATLQQTFVVSDATKPDCPIMYASSGFFTMTGYSSKEVIGRNCRFLQGKDTDKNEVDKIRKAVKTGSSYCGRLYNYKKDGTPFWNLLTITPIKDDNGNTIKFIGMQVEVSKYTEGVNEKELRPNGLPKSLIRYDGKETHAAFAMYTME</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CQV3</accession>
    <name>A0AAD5CQV3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein FAR1-RELATED SEQUENCE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_013567</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006976">
        <property type="protein sequence ID" value="KAI7746453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CQV3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7746453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="157" mass="17372" checksum="DCF06DE5C0F0484B" modified="2024-05-29" version="1">MARWKRDVLPRSVFSIDARYGVDHSATAVMRNGLLEMFNQCVDRLSGLPEMLRAFSEQFQGMKDEVIGKGVGDAEHDGANKHVIANLLRVPEEIEGSCSNPQGIRKKGCGTNQRLVGPGEKAANRKKKTTHQCKTCFEVVSHHDSRNCPSKNKDPAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CQW4</accession>
    <name>A0AAD5CQW4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_031834</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007031">
        <property type="protein sequence ID" value="KAI7746144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CQW4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7746144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="63" mass="7356" checksum="7ECDD35F1BD25683" modified="2024-05-29" version="1">MEMGFNLHTVMQHWISLQKLLTDYHNSSSESNREPHLTSEQQELMEMCVLPEINELNINLEAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CSR6</accession>
    <name>A0AAD5CSR6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Hexosyltransferase</fullName>
            <ecNumber evidence="13">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">M8C21_002019</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">AA19_3_7</strain>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Golgi apparatus membrane</location>
            <topology evidence="2 13">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the glycosyltransferase 31 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="13"/>
    <dbReference type="EMBL" id="JAMZMK010006813">
        <property type="protein sequence ID" value="KAI7747227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CSR6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008378">
        <property type="term" value="F:galactosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025298">
        <property type="entry name" value="DUF4094"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002659">
        <property type="entry name" value="Glyco_trans_31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214">
        <property type="entry name" value="BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214:SF124">
        <property type="entry name" value="HYDROXYPROLINE O-GALACTOSYLTRANSFERASE HPGT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13334">
        <property type="entry name" value="DUF4094"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01762">
        <property type="entry name" value="Galactosyl_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10 13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12 13" id="KW-0464">Manganese</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="37"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4094" evidence="15">
        <location>
            <begin position="39"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF13334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAI7747227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="291" mass="33079" checksum="87D5B23C6FACF23E" modified="2024-05-29" version="1">MDNSPPYYNKEGLPLSTTKSSKSDKQRFRSSSSSRSSIPSIFFAFFSCVAWLYIAGRLWQDAENRTLLANLLMQNSAERPKVLTVEDKLVVLGCKDLERKIVEAEMEITLAKSQGFLKDRLKQPGLSSSKKLLAVIGVYTGFGSRLSRNVFRGSWMPKGDSLKKLEERGIVIRFVIGRSPNRGDSLDRNIDEENRTTKDFLILDGHEEADEESPKKAKFFYSTAVQNWDAEFYVKVDNNIALDLEGLIELLESRRGQDSVYIGCMKSGEVVAEEGKPWYEPDWWKFGDEKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CT48</accession>
    <name>A0AAD5CT48_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Succinate dehydrogenase subunit 7A, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_002310</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006763">
        <property type="protein sequence ID" value="KAI7747389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CT48"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045273">
        <property type="term" value="C:respiratory chain complex II (succinate dehydrogenase)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034573">
        <property type="entry name" value="SDH7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36041">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE SUBUNIT 7A, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36041:SF2">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE SUBUNIT 7A, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7747389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="96" mass="10817" checksum="3A7D7D5DA0D7E601" modified="2024-05-29" version="1">MASFLKSNTFSTLRPHHLQGLPSQSRRQLHVEPGAREKALLAKDPALERFKSYRKGAASIRRIGDYLTIAVVAGCCYEIYVRAVTREEARKALKSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CU20</accession>
    <name>A0AAD5CU20_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">Pyrrolo-quinoline quinone repeat domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_015343</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyrroloquinoline quinone</name>
            <dbReference type="ChEBI" id="CHEBI:58442"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial PQQ dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006983">
        <property type="protein sequence ID" value="KAI7746435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CU20"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.140.10.10">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018391">
        <property type="entry name" value="PQQ_b-propeller_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002372">
        <property type="entry name" value="PQQ_rpt_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32303:SF10">
        <property type="entry name" value="OUTER MEMBRANE PROTEIN ASSEMBLY FACTOR BAMB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32303">
        <property type="entry name" value="QUINOPROTEIN ALCOHOL DEHYDROGENASE (CYTOCHROME C)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01011">
        <property type="entry name" value="PQQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13360">
        <property type="entry name" value="PQQ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00564">
        <property type="entry name" value="PQQ"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041923874" description="Pyrrolo-quinoline quinone repeat domain-containing protein" evidence="4">
        <location>
            <begin position="23"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="domain" description="Pyrrolo-quinoline quinone repeat" evidence="5">
        <location>
            <begin position="27"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="Pyrrolo-quinoline quinone repeat" evidence="6">
        <location>
            <begin position="423"/>
            <end position="502"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7746435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="529" mass="56663" checksum="8A4B23246E99066B" modified="2024-05-29" version="1">MAPNGLYFAILIWLFLIIQTSAKPTDWTNHGGDLQNRRYAYGESKISPKTVASLRLKWKFVAGNDVTATPAIYNNTIYFPSWNGNIYALNASDGSVIWQHNLGQLTGLNSTGLIQGVNWTVSRATPTVANDLLIVGLYGPAYILGLNRSNGEFVWSTQLDTHYAAYITMSGTYYKSAFYVGTSSAEEASSIEACCTFRGSFFKLNATNGKILWQTYMLPDNNGSTDEYAGAGIWGSSPSIDIHRNAVYIATGNMYSAPLNVTQCQEAQNNQTTPTEEDKCIEPDNHSNSILALDLDSGHINWYKQLGGYDLWFFACNNLSTPGCPPGPNPDADFAEAPMILTISDHGKKRDIVATVQKSGIAWALDRDNGSIVWSTEAGPGGAGGGGTWGSATDGKTVYTNIANSLRSNFTLKPSNITTTSGGWVSMDAATGNVLWSRENPSNATASGPVSLANGVVFAGSTNATGPIYAMSAATGEILWSYDTGATVYGGMSISDGCVYVGNGYKVNLGFFDPTYTSGDTLFAFCIFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CUW0</accession>
    <name>A0AAD5CUW0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Sugar phosphate transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_015894</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006475">
        <property type="protein sequence ID" value="KAI7748733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CUW0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004853">
        <property type="entry name" value="Sugar_P_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050186">
        <property type="entry name" value="TPT_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11132">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03151">
        <property type="entry name" value="TPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="84"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="123"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="177"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="199"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="230"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="269"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="296"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="321"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="Sugar phosphate transporter" evidence="7">
        <location>
            <begin position="60"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="357"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="363"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7748733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="381" mass="41868" checksum="06DBD4B7695AC556" modified="2024-05-29" version="1">MLGTKALFSFLSRKDVRRILKRKDSDAGERGRAMDELRASLFSKFRRQHQSLLGPTFALTFNFVVSVSIILMNKLVLVKVGFNYPIFLTFIHYIISWFIMALLKAFSLLPPPPSSKSTRFSSLLGLGIVMSLSTGLANVSLKYNSVGFYQMAKIAVTPAIVLAEFMLYRKRISFRKVLALTVVSVGVAVATVTDLQFHFFGACIAVAWIIPSATNKILWSNLQQQESWNALALMWKTTPITVFFLVMMMPSLDPPGVLSFDWSFYNTSIIAASAVLGFLLQWSGALALGETSATTHVVLGQFKTCVILLGGFIMFGSNPGSTSICGAVTALAGMSFYTHLNLRPQQQSLKASIRQSSLPKSKLGKENGDTHDHGNHGDESV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-10-08" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CWU7</accession>
    <name>A0AAD5CWU7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phytocyanin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_011782</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">May act as a carbohydrate transporter.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the early nodulin-like (ENODL) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006272">
        <property type="protein sequence ID" value="KAI7749828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000018">
        <property type="entry name" value="Lamin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039391">
        <property type="entry name" value="Phytocyanin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003245">
        <property type="entry name" value="Phytocyanin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33021">
        <property type="entry name" value="BLUE COPPER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33021:SF231">
        <property type="entry name" value="EARLY NODULIN-LIKE PROTEIN 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02298">
        <property type="entry name" value="Cu_bind_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51485">
        <property type="entry name" value="PHYTOCYANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042261310" description="Phytocyanin domain-containing protein" evidence="6">
        <location>
            <begin position="27"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="Phytocyanin" evidence="7">
        <location>
            <begin position="27"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7749828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="195" mass="21710" checksum="E4CE9CF3821FADB9" modified="2024-05-29" version="1" fragment="single">MGRTGSIWATAIAIAFLAIMVPQVSAKRYIVGGSMGWTTNVNYTLWAANQTFFFGDWLFFVYDRNQNDVIEVNQTNYETCNAEHPVHNYTTGAGRDVVPLNVTHDRYFISSKGSCYGGMKLHVHLSPLPPPPQAAPAKSDSSRFIVLRXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CYF2</accession>
    <name>A0AAD5CYF2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_012440</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006496">
        <property type="protein sequence ID" value="KAI7748640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CYF2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37744">
        <property type="entry name" value="STAR LIPID TRANSFER-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37744:SF1">
        <property type="entry name" value="STAR LIPID TRANSFER-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="14"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7748640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="128" mass="13514" checksum="0018AFDDD98FDAC1" modified="2024-05-29" version="1">MEKESNKGGGKGGYWWWGAASGVQLGWGIYSFRKGYSGDSRLMPLKAFAIASLFVGATATATLGTLRASGIHSVKDCIEAGASIRSSLGVQTDNNNLIGLNSFLEELQLLDFTLGTAAFGLGIVFNIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CYZ0</accession>
    <name>A0AAD5CYZ0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_028953</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006150">
        <property type="protein sequence ID" value="KAI7750482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CYZ0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34956">
        <property type="entry name" value="OS05G0397300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34956:SF2">
        <property type="entry name" value="OS05G0397300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="115"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7750482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="133" mass="15402" checksum="ABB43651EB073185" modified="2024-05-29" version="1">MEVVEDDLFFEDLSKRISLLIMDDDEDPTLYCPPVSFQVLSQTIHTNHNQIPSFNEQNGRREIKGTGVFIPQSSTNSRRSKQSRSKSSITRNHQSQTQSQKHVNASSGHFPHEPYNNQNTNYTYNSLNHKRCY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D0K3</accession>
    <name>A0AAD5D0K3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rhodanese domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_002864</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005932">
        <property type="protein sequence ID" value="KAI7751304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D0K3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01518">
        <property type="entry name" value="RHOD_YceA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022111">
        <property type="entry name" value="Rhodanese_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020936">
        <property type="entry name" value="TrhO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43268:SF3">
        <property type="entry name" value="RHODANESE-LIKE DOMAIN-CONTAINING PROTEIN 7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43268">
        <property type="entry name" value="THIOSULFATE SULFURTRANSFERASE/RHODANESE-LIKE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12368">
        <property type="entry name" value="Rhodanese_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="1">
        <location>
            <begin position="165"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7751304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="376" mass="42725" checksum="23FCB7062970F6A8" modified="2024-05-29" version="1">MGSLTLPCFPPINPITITRNTYYYSSYSIPIKTTNINYINIRLQPCKCTTPLSSSTTSTTTLEPNWVKGDEGLEENQRVFVVVNFYRFVSIKHPEDEVAKHLEFLQYSGPSEDALAYASWIKKDERFKDILVQMEGGVSHLPLVDSSMRATPLTPCEWRKKVSNFDKDVVLLDVRNGYEWDIGHFNGAQRPNVDCFRSTTFGLSESEDVASDPLANVDKENTDILMYCTGGIRCDVYSTILRQKGYKNLYTLKGGISHYLECEGPVEWIGNLFVFDSRLSLPPSLVKHDSTDHESIKRELDGNAVFAKCYICGSQVSELRHRNCANLDCNLLFLCCSSCMNDLRGCCTLKCTSAARLRPVLPGHERYKKWHHYRDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D119</accession>
    <name>A0AAD5D119_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Allantoate deiminase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_022925</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005912">
        <property type="protein sequence ID" value="KAI7751442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D119"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016813">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amidines"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006144">
        <property type="term" value="P:purine nucleobase metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010158">
        <property type="entry name" value="Amidase_Cbmase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001261">
        <property type="entry name" value="ArgE/DapE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32494">
        <property type="entry name" value="ALLANTOATE DEIMINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32494:SF19">
        <property type="entry name" value="ALLANTOATE DEIMINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001235">
        <property type="entry name" value="Amidase_carbamoylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="Bacterial exopeptidase dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00758">
        <property type="entry name" value="ARGE_DAPE_CPG2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0464">Manganese</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0659">Purine metabolism</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7751442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="402" mass="43481" checksum="C5987E194B2B33EA" modified="2024-05-29" version="1">MASINNLSIPFFIFVSLFPIVNLLLLSPLLTAASSIISDGLKIDLFPEILRDEAVARLYQLGKVSDADGYLERTFLSPASVRAGTLIRTWMEDAGLTTWVDGLGNVHGRIQPPNASKKTLLIGSHLDTVVDAGMFDGALGIITAISAIKVLNVTGRLKHLRHPVEVIAFSDEEGVRFQSTFLGSAAIAGVLPVATLQIPDKSGLTVQNVLKENLIETTEENLNLLKYEPESVLGYIEVTVKGSQGHAGTVPMSMRQDPMVAAAELIVTFTVDIRTIDDTGREAIIYELSNQMHSICDRRSVSCLMERKHDADAVKCDSELNSQLKSAAYSAVKRMTGEDIGDVPVLMSGAGHDAMAMSHLTNVGMLFVRCRGGISHSPEEHVLDDDVWVAGLAIQAFLETNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D705</accession>
    <name>A0AAD5D705_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Sugar phosphate transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_009584</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010004554">
        <property type="protein sequence ID" value="KAI7754069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D705"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004853">
        <property type="entry name" value="Sugar_P_trans_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03151">
        <property type="entry name" value="TPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sugar phosphate transporter" evidence="1">
        <location>
            <begin position="36"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7754069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="73" mass="8190" checksum="05C18170E98A29B3" modified="2024-05-29" version="1" fragment="single">MIMFTASEGLPKTNNRHQRSINGLYKSFHCYGAHPRQTVFSNVCLGKVAVSFSHIIIAMEPYLSVILSAMFRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D706</accession>
    <name>A0AAD5D706_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Heat stress transcription factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_012595</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the HSF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010004258">
        <property type="protein sequence ID" value="KAI7754177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D706"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000057">
        <property type="entry name" value="Heat shock transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000232">
        <property type="entry name" value="HSF_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015">
        <property type="entry name" value="HEAT SHOCK TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015:SF337">
        <property type="entry name" value="HEAT STRESS TRANSCRIPTION FACTOR A-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00447">
        <property type="entry name" value="HSF_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00056">
        <property type="entry name" value="HSFDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00415">
        <property type="entry name" value="HSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00434">
        <property type="entry name" value="HSF_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0346">Stress response</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HSF-type DNA-binding" evidence="13">
        <location>
            <begin position="92"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="154"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00055747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00081483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7754177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="389" mass="43828" checksum="493295CB294C2612" modified="2024-05-29" version="1" fragment="single">SDPFSLGWSPQPVSSHLPLPVSSAAGEATVTIAERDTIPQPLESLQEPQIPPFLSKTFDLVDDPRLDPIISWGASGNSFVVWDPIEFARIILPRHFKHNNFSSFVRQLNTYGFHKIDPDKWEFANRSFLRGQRHLLKNIQRRKSNQSSSSSSVLANQEVNNMLIEAEIERLHKEKREMMQQIIELKTGNIETHQHMESVNQKLEAAEEKQKQMVSFLAQVFENPTFVSSIRAKTEETLRISSPRTTRKFVKHQHIGGTSRSLEFGTKPEDFGSKGKNVMAIEPEGAPEYLLDPNSGGIDDIVVKQENIWSDIGNYELPELPPELAPELVPELAPELAPELPEIRAGGGELTDDLWNLGTSGGENWVDDDICFDDILENQEQDSSKKMDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D7T3</accession>
    <name>A0AAD5D7T3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Receptor-like serine/threonine-protein kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_021510</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010002874">
        <property type="protein sequence ID" value="KAI7754642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D7T3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00028">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.90.10.30:FF:000003">
        <property type="entry name" value="Os04g0303100 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000178">
        <property type="entry name" value="serine/threonine-protein kinase PBS1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.90.10.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001480">
        <property type="entry name" value="Bulb-type_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036426">
        <property type="entry name" value="Bulb-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051343">
        <property type="entry name" value="G-type_lectin_kinases/EP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47976">
        <property type="entry name" value="G-TYPE LECTIN S-RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE SD2-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47976:SF30">
        <property type="entry name" value="RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01453">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08276">
        <property type="entry name" value="PAN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00108">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51110">
        <property type="entry name" value="alpha-D-mannose-specific plant lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50927">
        <property type="entry name" value="BULB_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="356"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="Bulb-type lectin" evidence="10">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="418"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="446"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7754642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="624" mass="69783" checksum="7AA1714649E4A753" modified="2024-05-29" version="1">MGYPQVVWSANRDHPVSDGAILTLTSTGGLVLQDVDGSTVWTTNTTGKSVAGLNLTDDGNLVLFDINSFVVWQSFDYPTDCLLLGQRLSQGQQLIPSVSSTDWTAQKGLFSLQVTDKGLFAYVGSNPPQAYYSYAVSGNDTNKGKRYVTFLNGSLSLFTLTAEPSNPDGVITIPQASTTQYIKLMPDGHLKLFEWQAGSWLVVADPFNSYLEGCKNPLACGRNALCLGNQRDQPCKCLPGDFIAVDYQKPHLGCTEITPLKCNATQDQTWYPIQNVAYFNIIADMENVDIETCKQACVNNCSCKAAIFRYGLDASYGDCYLPYELFTMMTFPYVIHRNASAFIKVRIPSLSPYSKLSLAGIILISTIVSVVFLLGVIGFLIYLICKRRRATLMEEEPIGELPGMPNRFYYEELKTATDNFNKKLGEGGFGAVYEGTLEDGSKIAVKCLEGLGQVNKSFLAEVQSIGSIHHVNLVRLRGFCAWKSQRFLVYEFMSNGSLDQWIYHRDQEHILEWECRKKIILDVAKGLAYLHEDWMLLDMVDKYSENMQTHALEVVEMMKVASWCLQYDYTKRPSMSTVVKALEGVIEVESRLDYNFTDPRTQNTIVEQEKDMTPLMGSVLSGPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D8A4</accession>
    <name>A0AAD5D8A4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">UTP--glucose-1-phosphate uridylyltransferase</fullName>
            <ecNumber evidence="3">2.7.7.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_026017</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>alpha-D-glucose 1-phosphate + UTP + H(+) = UDP-alpha-D-glucose + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19889"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.7.7.9"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UDPGP type 1 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.9" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010001697">
        <property type="protein sequence ID" value="KAI7755147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D8A4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003983">
        <property type="term" value="F:UTP:glucose-1-phosphate uridylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006011">
        <property type="term" value="P:UDP-alpha-D-glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01425">
        <property type="entry name" value="RPS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.10.10:FF:000001">
        <property type="entry name" value="UTP--glucose-1-phosphate uridylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001865">
        <property type="entry name" value="Ribosomal_uS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018130">
        <property type="entry name" value="Ribosomal_uS2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023591">
        <property type="entry name" value="Ribosomal_uS2_flav_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002618">
        <property type="entry name" value="UDPGP_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016267">
        <property type="entry name" value="UDPGP_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43511">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00318">
        <property type="entry name" value="Ribosomal_S2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01704">
        <property type="entry name" value="UDPGP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00395">
        <property type="entry name" value="RIBOSOMALS2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52313">
        <property type="entry name" value="Ribosomal protein S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00962">
        <property type="entry name" value="RIBOSOMAL_S2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7755147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="562" mass="62855" checksum="24F368A669F27F4B" modified="2024-05-29" version="1">MTIHSAVIQKLLQTNAHLGRRVAENHYKIYTYGSRNGTTIIDSDKTLICMRSACNFIGNLAKLNARFLFVNTNPLLDEIIQQMIINTGCRNDNSWRLGGFLTNRFSPKRFRSRNQKFNLTSVNRPDCVVIFDTERKSSVILEATRLHIPIVGLVDPSMPLETYKKITYPVPANDSVQFVYLFCNLITKTIQYEQKKLAAAKPKVVVKEEEAKVGEEALPAGKIKIDGVDEELLVLRYEGLSPASDDPLEIKELLEKLVVVKINDNTGTKMGFDGPKSAVEIANGLTSLDLLANYIESLNSKYGCNVPLLLVDDANTHDKTLQIVEKQSNKNIICLVKDQHQDDDVKISFDQREALLSLKKSGKLDDLLLQGKEFILMLNSNNLAEVVDLNILNHLITNKIKYCMEIEERFRGTTTLNTWLNINSITNHPETKLFGPPIAVTVPVSQNLPLEEASDLLIFKSDLYSCVNGTLIRNEARISPFNPVIELGPEFEKVADFESRFESIPSIIDLDSLKVSGDVWFGSDVILKGLVSINARPGEKIVIPDGAVLENKSINSQQDVPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D8B7</accession>
    <name>A0AAD5D8B7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Vignain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_018803</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010002455">
        <property type="protein sequence ID" value="KAI7754804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D8B7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02248">
        <property type="entry name" value="Peptidase_C1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000023">
        <property type="entry name" value="Senescence-specific cysteine protease SAG39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025661">
        <property type="entry name" value="Pept_asp_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025660">
        <property type="entry name" value="Pept_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039417">
        <property type="entry name" value="Peptidase_C1A_papain-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013201">
        <property type="entry name" value="Prot_inhib_I29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08246">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00705">
        <property type="entry name" value="PAPAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00848">
        <property type="entry name" value="Inhibitor_I29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00640">
        <property type="entry name" value="THIOL_PROTEASE_ASN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00639">
        <property type="entry name" value="THIOL_PROTEASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0788">Thiol protease</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042162338" description="Vignain" evidence="8">
        <location>
            <begin position="23"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="Cathepsin propeptide inhibitor" evidence="10">
        <location>
            <begin position="40"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="9">
        <location>
            <begin position="128"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="344"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="347"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7754804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="363" mass="40308" checksum="9849DE084BA3B5B8" modified="2024-05-29" version="1">MAINKFLLVSLSLSLLLGVAWSFDFHEKELETDDGLWDMYERWRSHHKVATNHDEKQRRFNVFKSNVLHVHETNKMDKPYKLKLNKFADMTNHEFKSVYAGSKIRHHRSLQGDRIGSKTFMYANVKSVPTSVDWRKKGAVNPVKDQGQCGSCWAFSTVAAVEGINKIKTNELVSLSEQELVDCDTLENQGCNGGLMDLAFDFVKKTGGLTREDAYPYTAADGKCDSNKMNAPVVSIDGHEDVPKNNEQALMQAVANQPVAVAIDAGGPDFQFYSEGVFTGSCGTELDHGVAAVGYGTTLDGTKYWIVRNSWGPEWGEKGYIRMQRGISDERGLCGIAMEASYPIKNSADNPKSSPTSSLKDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D8H0</accession>
    <name>A0AAD5D8H0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Endoglucanase</fullName>
            <ecNumber evidence="9">3.2.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_026142</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>Endohydrolysis of (1-&gt;4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 8 9">Belongs to the glycosyl hydrolase 9 (cellulase E) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.4" evidence="9"/>
    <dbReference type="EMBL" id="JAMZMK010000421">
        <property type="protein sequence ID" value="KAI7756376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D8H0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008810">
        <property type="term" value="F:cellulase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000020">
        <property type="entry name" value="Endoglucanase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001701">
        <property type="entry name" value="Glyco_hydro_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018221">
        <property type="entry name" value="Glyco_hydro_9_His_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22298">
        <property type="entry name" value="ENDO-1,4-BETA-GLUCANASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00759">
        <property type="entry name" value="Glyco_hydro_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00592">
        <property type="entry name" value="GH9_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8 9" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4 9" id="KW-0136">Cellulose degradation</keyword>
    <keyword evidence="6 8 9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3 8 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 8 9" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycoside hydrolase family 9" evidence="10">
        <location>
            <begin position="3"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="383"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7756376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="445" mass="49439" checksum="47D141BD44DB8669" modified="2024-05-29" version="1" fragment="single">HDYSDALRKCILFFEGQRSGRLPPDQRVTWRGHSALHDGASVGVDLRGGYYDAGDNIKFGFPMAFTTTMLAWSVIDFGRLMGAELGNAIKAVKWGTDYLLKATAIDGVVFVQVGDPISDHNCWERPEDMDTLRTAYRIDRNHPGSDVAGETAAALAAASIVFRSRDPVYSRILLNRAVKVFRFADTYRGAYSDYLSSAVCPFYCDNNGYQDELLWAAAWLHKASRRRQYREYIVRNEVILRAGDTINEFGWDNKHAGINVLISKEVLMGRSPNLKSFKNNADAFICSILPGTAHLQVQYSPGGLIFKTGGSNMQHVTSLSFLLLAYSNYLSHANHVVPCGDKIASPALLKSLARRQVNYILGDNPMRMSYMVGYGPRYPQRIHHRGSSLPSVRIHPSHIGCKAGSRYFFSPDPNPNVLVGAVVGGPNTTDAFPDSRPFFQETEPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D8T0</accession>
    <name>A0AAD5D8T0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Transmembrane 9 superfamily member</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_008751</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the nonaspanin (TM9SF) (TC 9.A.2) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000352">
        <property type="protein sequence ID" value="KAI7756548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D8T0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072657">
        <property type="term" value="P:protein localization to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004240">
        <property type="entry name" value="EMP70"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10766:SF164">
        <property type="entry name" value="TRANSMEMBRANE 9 SUPERFAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10766">
        <property type="entry name" value="TRANSMEMBRANE 9 SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02990">
        <property type="entry name" value="EMP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="354"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="419"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="455"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="496"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="523"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="578"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="610"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="645"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="689"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="721"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7756548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="721" mass="81993" checksum="AEDC999AB758446E" modified="2024-05-29" version="1" fragment="single">IPPHTPLLYTLSIPLPNISSTITDEIQDRHFVEHLISPSHIPTTNSSFTNLFHCFHHIRSNKKKMKPFEHFKIWGLLICLISQLGHGYYLPGSYPHKYVVGDQLSVKVNSLTSIETEIPYSYYSLPFCKPQEGVKDSAENLGELLMGDRIENSPYKFKMHTNETEIFLCQTKPLSTDEFKLLTTRIDEMYQVNVILDNLPAIRYTKRDNFLVRWTGYPLGIKVQETYYVFNHLKFTVLVHKYEETNVASVMGTGDAAEVIPTVDKPGSDIPGYIVVGFEVQPCSFQHNKESLKNLKMYGKYPSKITCETNTVTMGIKENEPVAFTYEVEFVESDIKWPSRWDAYLKMDGAKVHWFSILNSLMVVTFLAGIVLVIFLRTVRRDLTHYEELDKEAQAQMNEELSGWKLVVSDVFRVPDYPALLCVMVGDGVQILGMAVVTILFAALGFMSPASRGTLLTGMLFFYMVLGILAGYVAVRMWRTIFSGDHKGWVSVCWKVSCFFPGIAFLILFVLNFLLWGSGSTGAIPFLLFVVLILLWFCVSVPLTLVGGYFGAKAPHIEYPVRTNQIPREIPAQKYPSWLLVLGAGTLPFGTLFIELFFIMSSIWMGRVYYVFGFLFIVLILLVVVCAEVSLVLTYMHLCVEDYKWWWKSFFASGSVALYMFLYSINYLVFDLKSLSGPVSATLYLGYSLFMVLAIMLATGTVGFLSSFWFVHYLFSSVKLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D963</accession>
    <name>A0AAD5D963_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">H15 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_028767</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the histone H1/H5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000265">
        <property type="protein sequence ID" value="KAI7756766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D963"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031492">
        <property type="term" value="F:nucleosomal DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030261">
        <property type="term" value="P:chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045910">
        <property type="term" value="P:negative regulation of DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00073">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005819">
        <property type="entry name" value="H1/H5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005818">
        <property type="entry name" value="Histone_H1/H5_H15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11467">
        <property type="entry name" value="HISTONE H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11467:SF131">
        <property type="entry name" value="HISTONE H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00538">
        <property type="entry name" value="Linker_histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00624">
        <property type="entry name" value="HISTONEH5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00526">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51504">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="4 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="H15" evidence="8">
        <location>
            <begin position="40"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="107"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="108"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="120"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="153"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="211"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="236"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7756766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="261" mass="27671" checksum="353353B914C18428" modified="2024-05-29" version="1">MSTEEPIASTEAPEAPKVETKVKKTKKASSTPRKAKETARHPPYFEMIKKAIVTLKERNGSSQRAIEKFIEEKEKNLPKIFKKSLLTQLKKSVAEGKLVKVKASYKLPSSRPASVSAPAKKTKKKPAVKAKPKTETKKTPAKKKPAAAAAKPVKAKAPVKAKTKPATKAKPVAKAKPASKAKPVAKAKPAAKAKVAVKAKPKTPVKKKAAAKPAAKVARTSTRTTPGKKAAAPKAAVKKTPVKKVAAKKTAAATRKGRGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DAK1</accession>
    <name>A0AAD5DAK1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">TATA-box-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_008204</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TBP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000111">
        <property type="protein sequence ID" value="KAI7757508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DAK1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04516">
        <property type="entry name" value="TBP_eukaryotes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.10:FF:000001">
        <property type="entry name" value="TATA-box-binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.10:FF:000002">
        <property type="entry name" value="TATA-box-binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.10">
        <property type="entry name" value="TATA-Binding Protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00408">
        <property type="entry name" value="TATA_bind_prot_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000814">
        <property type="entry name" value="TBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030491">
        <property type="entry name" value="TBP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012295">
        <property type="entry name" value="TBP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033710">
        <property type="entry name" value="TBP_eukaryotic"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10126">
        <property type="entry name" value="TATA-BOX BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00352">
        <property type="entry name" value="TBP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00686">
        <property type="entry name" value="TIFACTORIID"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55945">
        <property type="entry name" value="TATA-box binding protein-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00351">
        <property type="entry name" value="TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7757508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="250" mass="28376" checksum="BE97A1C75DAC2002" modified="2024-05-29" version="1">MADQVLEGGSQPVDLSKHPSGIVPTLQNIVSTVNLDCKLDLKAIALQARNAEYNPKRFAAVIMRIRDPKTTALIFASGKMVCTGAKSEQQSKLAARKYARIIQKLGFPAKFKDFKIQNIVGSCDVKFPIRLEGLAYSHGAFSSYEPELFPGLIYRMKQPKIVLLIFVSGKIVLTGAKVRDETYTAFENIYPVLTEFRKNQQWFVTSLSFYFIFSCNKLDRFLNCHHKLFSIQRVFFFSSHKNNIIVKMFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DAW2</accession>
    <name>A0AAD5DAW2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Ras-related protein RABC2a</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_032085</name>
        <name evidence="13" type="ORF">M8C21_032088</name>
    </gene>
    <organism evidence="12 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Intracellular vesicle trafficking and protein transport.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000085">
        <property type="protein sequence ID" value="KAI7757681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAMZMK010000085">
        <property type="protein sequence ID" value="KAI7757684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DAW2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000456">
        <property type="entry name" value="Ras-related protein RABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0636">Prenylation</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00060176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7757681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7757684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="222" mass="24819" checksum="0043B112B1743DCB" modified="2024-05-29" version="1">MSGEIKGGWKRSQISPTSYDYSFKILVIGDSGVGKSSLLLSFISSSDHPLQHISPTIGVDFKMKMLAVEGKRLKLTIWDTAGQERFGTLTSSYYRGAHGIILVYDVTRRETFTNLSEIWAREVDLYSTNPDCVKILVGNKVDRDVERAVTVEEGMALAKAHGCLFYECSARTRANVQQCFKDLSLKILDKPGLLEQGSVVVKRQILKEKQLSEKKRSDNCCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DBY4</accession>
    <name>A0AAD5DBY4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">M8C21_013702</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="23">AA19_3_7</strain>
            <tissue evidence="23">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the leguminous lectin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="EMBL" id="JAMZMK010000005">
        <property type="protein sequence ID" value="KAI7758341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DBY4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002229">
        <property type="term" value="P:defense response to oomycetes"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06899">
        <property type="entry name" value="lectin_legume_LecRK_Arcelin_ConA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000342">
        <property type="entry name" value="L-type lectin-domain containing receptor kinase VIII.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050528">
        <property type="entry name" value="L-type_Lectin-RKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001220">
        <property type="entry name" value="Legume_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27007">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00139">
        <property type="entry name" value="Lectin_legB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0430">Lectin</keyword>
    <keyword evidence="15 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-0675">Receptor</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9 21" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042273903" description="non-specific serine/threonine protein kinase" evidence="21">
        <location>
            <begin position="27"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="294"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="22">
        <location>
            <begin position="355"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="662"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="662"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="673"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="384"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="KAI7758341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="688" mass="77229" checksum="57E3A5F211365F14" modified="2024-05-29" version="1">MTSTKHLVFLFSTLLTTSLLPNPSTTHPRTTTFHFPPFPLHNLTFLGDAHHLTTTTTTLTLDSPFPSSGTIIYTTPIRFTDTRTNSTTSFSTSFSFSILNLNRDFGGGFSFFISPLNNTLGSPGPYLGLLNSSHLTLNKFIAIEYDTRLDPYFNDPNDNHVGLDIDSLNSIQTADCGSLGIDLKTGFSFTSWVDYVHDRRTLEVFLTRGSGFKPEYPLLNVSIDLSGYFQEVMYLGFSGSTDGSQETHSIDHWSFRSFGIKRFKPITDNPNNVSTDMVPVRFPGRLNNNRKRNFGFGFGLEVSGSVFIFAGLVIFGYVSVKKWREVKTEMNIKAEVARNPREFSYKELKIATNNFHSSHIIGHGSFGTVYKAFIMTSGTPAAVKRSLRSGEAKAEFLAELSAIAGLRHKNLVQLLGWCVKRGELLLVYELMPYGSIDNVLYQDPTHWAFLKWPNRYKIATGLASVLAYLHQECDKLVIHRDIKSSNVMLDSNLNPRLGDFGLARLIDHDKSPVTTLTAGTAGYLAPEYLHYGKATDKTDVYSYGVVVLEVCCGRRPIEREIGGLRMVNLVDWVWDLHANGAVLDAIDKRLKGEFDEEEGKRLLMVGLSCVNPRSDMRPSMRRVLQVLNREAEEMEVPSVKPKVSFSSGMPLSVDDLMRYVEQDEQDLVHSEEHEEENGSDSEFEIRID</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DC62</accession>
    <name>A0AAD5DC62_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_018689</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000036">
        <property type="protein sequence ID" value="KAI7757968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DC62"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017053">
        <property type="term" value="C:transcription repressor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028226">
        <property type="entry name" value="LIN37"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37173">
        <property type="entry name" value="HYDROXYPROLINE-RICH GLYCOPROTEIN FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37173:SF1">
        <property type="entry name" value="PROLINE-RICH FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15306">
        <property type="entry name" value="LIN37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="151"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7757968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="221" mass="24636" checksum="13EAB151A6D031E6" modified="2024-05-29" version="1">MSSPPPPPNPLPTTTTFLKPTKHSHPSIYKTFYNPYTVNGSHGLLNLDHLVNPLSAPLQQTLVGGGHSVSANMKVVGQSPVAADNSVRDKNIDDAFVIIRDRKVQVSEGASLYAQCRSWLKNGFTLEKQPQYPDCVKSLPKPLPASTVEARKEDDAEIEEKTENVDHLSAKELLQLHVRRAKKVRARLRNQRLQRIERYKDRLALLLPAVADQQPKNNPAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DHC2</accession>
    <name>A0AAD5DHC2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="4">3.4.19.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Deubiquitinating enzyme MINDY-3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_010832</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Hydrolase that can remove 'Lys-48'-linked conjugated ubiquitin from proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MINDY deubiquitinase family. FAM188 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="4"/>
    <dbReference type="EMBL" id="JAMZMK010000083">
        <property type="protein sequence ID" value="KAI7757700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DHC2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990380">
        <property type="term" value="F:K48-linked deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071108">
        <property type="term" value="P:protein K48-linked deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025257">
        <property type="entry name" value="MINDY-3/4_CD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039785">
        <property type="entry name" value="MINY3/4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003903">
        <property type="entry name" value="UIM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12473">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE MINDY-4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12473:SF8">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE MINDY-4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13898">
        <property type="entry name" value="MINDY-3_4_CD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02809">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01174">
        <property type="entry name" value="DUF4205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Deubiquitinating enzyme MINDY-3/4 conserved" evidence="8">
        <location>
            <begin position="118"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="560"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="22"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00033208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7757700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="620" mass="67048" checksum="F20B11D4205DDFE0" modified="2024-05-29" version="1">MEDHMEDDLQKALRMSMQYEPPEAKRSKTPVEESPEVKNRRELMAAAAEKRMMAATASASVSVSTAPSAVVSSGVGSSVGKSVGSESSEMKVEESVVVMKEEERSGEELAVEKANQLFSMVFGNGVSKGILAQWSNQGIRFSPDEETSMGLVQHEGGPCGVLAAIQAFVLKYLLFFPQELVKDGSKTPIISVSKRLQETQDSATNVFGSLTEETKSRALVKSMGEILFLCGNNNSATIASLRVLDHEIEGKDESSKDEIVARSLDGLSIESGDDLQKALKVSTFTSLSSAMEKLEEIIPIFRSRMGALLFLLSALLSRGLEAVQADRDDPTQPLVTAPFGHASQEIVNLLLSGMAVANVFDGKMDLGGGMFVKGILTPVEVGFLTLLESLNFCKVGQSLKSPKWPIWVIGSESHYTVLFALDPRVQDENEFESRETMIRRAFDAQDQSGGGGFISVEGFHQVLKDANVNLPADKVNHLCSSGFIVWSEFWQVLLDLDKNLGGLKDSTGLMGKKVFDLYHFNGIAKSVLGGSGGETPVQRPRLTKLRVCVPPRWTPEEFMANVSLPGSSSGAGGGDSSGTQDAMSEVSKPERAQHAPLVDCIRTRWARAVCNWEGDPPSIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G141</accession>
    <name>A0AAD5G141_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Carotene epsilon-monooxygenase, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_003316</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012189">
        <property type="protein sequence ID" value="KAI7724554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G141"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016117">
        <property type="term" value="P:carotenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11046">
        <property type="entry name" value="CYP97"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050196">
        <property type="entry name" value="Cytochrome_P450_Monoox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291:SF134">
        <property type="entry name" value="CAROTENE EPSILON-MONOOXYGENASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0349">Heme</keyword>
    <keyword evidence="3 4" id="KW-0408">Iron</keyword>
    <keyword evidence="3 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="2 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="3">
        <location>
            <position position="514"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="576"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7724554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="576" mass="64925" checksum="97CAA385040E1252" modified="2024-05-29" version="1" fragment="single">QNCKLTNRSRFDFQLQAESLLPLSKKIIISKVNFKHHPQNNMPTVHSLSPLITTINHHNHHRFPTTQPKPLLQPTTHCPFTIKSSLEPKPTSKNGSWVSPDWLTSLVRSVTFTGTDDSNIPIASAQLDDVSDLLGGALFLPLFKWMNDYGPIYRLAAGPRNFVIVSDPQIAKHVLRNYGSIYAKGLVAEVSEFLFGSGFAIAEGSLWTARRRAVVPSLHKKYLSVIVDRVFCKCSERLVEKLRSYARDGTSVNMEQQFSQLTLDVIGLAIFNYNFDSLTADSPVIESVYTALKEAEAHKSIMQIKAEQAVTVIRETVEELIMKCKEIVEKEGEKIDDEDYVNDADPSILRFLLASREEVSSQQLRDDLLSMLVAGHETTGSVLTWTAYLLSKDPSSLMKAQEEVDRVLRGRRPTYEDIKNLKFITRCINESMRLYPHPPVLIRRAIVADELPGNYKVNPGQDIMISVYNIHHSSKVWERAEEFIPERFGLDGPVPNESNTDYKFIPFSGGPRKCVGDQFAMLEAIVALAIFLQNMEFELVPNQKINMTTGATIHTTSGLYMKVKERKVNSMAMSSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G1L4</accession>
    <name>A0AAD5G1L4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PGG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_025679</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012218">
        <property type="protein sequence ID" value="KAI7724451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G1L4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026961">
        <property type="entry name" value="PGG_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24177:SF475">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING DOMAIN, PGG DOMAIN PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24177">
        <property type="entry name" value="CASKIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13962">
        <property type="entry name" value="PGG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="492"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="351"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="domain" description="PGG" evidence="3">
        <location>
            <begin position="699"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7724451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="748" mass="84877" checksum="9363D78C10C30D95" modified="2024-05-29" version="1" fragment="single">MNEKVLNDLVDLGTAQEIWMKLESLFSSPVRDTEEGYSYNALDFIAPAVSSIREDLKYIQASSVNAVSSIREDLKYIQASSVNVSSFVTVKLSGRHNYEIWKAQMICLMESQELLHILGDHYPFPRDKGAHMIWLYDRLVKGWIFGSMEERVLNNFTHFDFARDVWTQLQLTFTSATIGDSLNAREELKYTQATHVNVSNFVSVKLSGRSNYKIWKAQMLGLIDSQDLLHFINNEIPFPGDANVHFLCGLYKRYDQLVQGWIFSSLNERLLKKFLNSESAIGLWMRLESTFYPPTSDGEDSLGFMFGIESVRDVPDTKRLLYKAAVEGCWWKAKSILKVETNAATEPVTANGNTILHLAVEMGHNYFVEKLLEHLKDGNGIETQNKKGRTALYSAARVGNEYAAQLLVQKRKELLLIKDNNGESPLDSANISNKFNISTYMLKTAPPSTWKAANRAIQTAIIGKQYDLAGDLLVKFPSCASTRVPIATLYPIYQLICFLILVLRLPFSMLYFLLWKVLAFTVPPIKNIERKKKEYKEAKNILSLICHQMGTSNPSYSYPLFEAVRQDTYEVVDEILFRSPSTFKYKDEEGHNIVQLAVINRSKKVYNLIHHIIEHTESYRTMIDSSENNLVHLAGRLAPANVLGRTTGAALQLQQELLWRAEVEKLMLPLELTKLNTFEETPAMVFTREHHDLMKEGENWMKTTAESCSITAALIVTVVFAAAITVPGGSNQESGIPVFEKEIAFTIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G1R0</accession>
    <name>A0AAD5G1R0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Peroxisomal membrane protein PMP22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_005689</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the peroxisomal membrane protein PXMP2/4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012217">
        <property type="protein sequence ID" value="KAI7724468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G1R0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007248">
        <property type="entry name" value="Mpv17_PMP22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 2, PXMP2 MPV17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266:SF86">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PMP22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04117">
        <property type="entry name" value="Mpv17_PMP22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="154"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7724468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="183" mass="21387" checksum="4655C65C5C9B5F5A" modified="2024-05-29" version="1">MSIAKRGFQHYLLQLQQHPLRTKAITAGVLSAISDIVSQKLSGIQKLQIRRLLLKVLFGFAYLGPFGHYYHILLDKLFKGKKDSKTVAKKVVVEQLTSGPWNNLIFMLYYGFVIEGRQWIHVKSKIKKEYPTVQYAGWTFWPVIGWINHRHVPLQFRVIVQSFVAMCWYGYILEFKSKIKDID</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G226</accession>
    <name>A0AAD5G226_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_018023</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011800">
        <property type="protein sequence ID" value="KAI7725964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G226"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006734">
        <property type="entry name" value="PLATZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31065">
        <property type="entry name" value="PLATZ TRANSCRIPTION FACTOR FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31065:SF11">
        <property type="entry name" value="PROTEIN RGF1 INDUCIBLE TRANSCRIPTION FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04640">
        <property type="entry name" value="PLATZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="162"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="225"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7725964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="244" mass="27939" checksum="81E89D7D99557359" modified="2024-05-29" version="1">MGIRKPAWLEALFNQKFFQACTIHENSKKNEKNICCVDCCISICPHCVHIHRFHRLLQVRRYVYHDVVRLEDLERLIDCSNVQAYTINSAKVVFIKKRPQNRQFKGSGNYCTSCDRSLQEPFIHCSLGCKVDFVLKHYRDLTPFLRVCKTLQLGPDFILPNDTGEDEMTNETPHSTIVDSDDPMSSGSGSFGSETMSIMCTGFVRKKRSGLYACERFSSKVNSEEDMATSMSRRKGIPQRSPMC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G2F5</accession>
    <name>A0AAD5G2F5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_004726</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011765">
        <property type="protein sequence ID" value="KAI7726055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G2F5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01837">
        <property type="entry name" value="SGNH_plant_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1110:FF:000003">
        <property type="entry name" value="GDSL esterase/lipase APG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051058">
        <property type="entry name" value="GDSL_Est/Lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035669">
        <property type="entry name" value="SGNH_plant_lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45648:SF58">
        <property type="entry name" value="GDSL ESTERASE_LIPASE LTL1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45648">
        <property type="entry name" value="GDSL LIPASE/ACYLHYDROLASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_4G14700)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042123764" evidence="4">
        <location>
            <begin position="21"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7726055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="359" mass="39239" checksum="5A9F6C6D54E643E7" modified="2024-05-29" version="1">MALVEVLTILLLVAMPKAHGARAFFVFGDSLVDNGNNNYLITDARADSPPYGIDYPTHRPTGRFSNGFNIPDLISQKLGAEPTLPYLSPELTGNKLLVGANFASAGIGILNDTGIQFASIIRIGQQLENFEAYQSQVSALIGPDQTQRLVNQALVLITLGGNDYVNNYFLAPVTARRLQYTIPQFTKYIISEYRKILMNLYNLGARRILVTGSGPLGCVPSQLALAAANGQCANEPQQAAALYNPQLVQMIQSLNQELGSDHFVAVNAMLMQNDFISNPQAFGFITSKVACCGQGPYNGVGICNPTSNLCDNREIYVFWDPFHPTERANKIIVETIYSGSEKYMHPMNLSTIMALDSMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G391</accession>
    <name>A0AAD5G391_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ribose-5-phosphate isomerase</fullName>
            <ecNumber evidence="4">5.3.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_022519</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>aldehydo-D-ribose 5-phosphate = D-ribulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:14657"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="ChEBI" id="CHEBI:58273"/>
            <dbReference type="EC" id="5.3.1.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate degradation; pentose phosphate pathway; D-ribose 5-phosphate from D-ribulose 5-phosphate (non-oxidative stage): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ribose 5-phosphate isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.6" evidence="4"/>
    <dbReference type="EMBL" id="JAMZMK010011410">
        <property type="protein sequence ID" value="KAI7727224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G391"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004751">
        <property type="term" value="F:ribose-5-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009052">
        <property type="term" value="P:pentose-phosphate shunt, non-oxidative branch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050262">
        <property type="entry name" value="Ribose-5P_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004788">
        <property type="entry name" value="Ribose5P_isomerase_type_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43748">
        <property type="entry name" value="RIBOSE-5-PHOSPHATE ISOMERASE 3, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43748:SF1">
        <property type="entry name" value="RIBOSE-5-PHOSPHATE ISOMERASE 4, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06026">
        <property type="entry name" value="Rib_5-P_isom_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75445">
        <property type="entry name" value="D-ribose-5-phosphate isomerase (RpiA), lid domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7727224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="295" mass="32182" checksum="0077AB4DEAAEB208" modified="2024-05-29" version="1" fragment="single">LHYTCYTPLNILSVAFAKMQFEDKCTMTLNVRGGQVLDVRQGNTNIQIGVAEGLDFLEIWFTAMKVDTYLQSGMVVGLGSGLASSMAIEYLGQKLRVGLLKDIVGIPTSDRIASEAEKAGIPLQQYQDNTQIDIAFNDADIVEEGSLNVVIGRQRPQGEESIVEEKKILDVTENLVIMVKEKQYKTAVEGSIPVSWMDTAEEIDDLFVGDAEVWRRPSMGHADPTGGNFPLVTREGHNILDVIFTTPIPNLAEVANLLNNIDGVACHGIITKTPCTTVIATESGLRIIENSNVSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G3I5</accession>
    <name>A0AAD5G3I5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_014354</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011719">
        <property type="protein sequence ID" value="KAI7726298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G3I5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7726298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="121" mass="13130" checksum="EC19B52B0821E539" modified="2024-05-29" version="1" fragment="single">MDSNRGSVSNKTANGMMLSRRNAEADGKGGLIGLLPSNPTASEVRTEENSLFGRQALTDANREREKTILKGMGYSPPSPSHSDTRTIDDNISEGGREWVEWHTDVLTKLNTLEMSTNGHAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G3W2</accession>
    <name>A0AAD5G3W2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Methyltransferase type 11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_022522</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011410">
        <property type="protein sequence ID" value="KAI7727227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G3W2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009820">
        <property type="term" value="P:alkaloid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051419">
        <property type="entry name" value="Lys/N-term_MeTrsfase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12176:SF66">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE-DEPENDENT METHYLTRANSFERASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12176">
        <property type="entry name" value="SAM-DEPENDENT METHYLTRANSFERASE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="4">
        <location>
            <begin position="53"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7727227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="147" mass="16956" checksum="182684FF210239A9" modified="2024-05-29" version="1">MLRDESACNTYNYGDSDYWDARYINESSFDWYQRYDALRPFLTQYIHTSSRVLMVGCGNAVISEDMVKDGYESIMNVDISCVAIEMMRRKYEHVPQLEYMQMDVRDMGFFLDDSFDSVIDKGTLDALMCGTDGPTSASQMLGEVSRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G4W7</accession>
    <name>A0AAD5G4W7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">O-acyltransferase WSD1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_016549</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011200">
        <property type="protein sequence ID" value="KAI7728502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G4W7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008374">
        <property type="term" value="F:O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019432">
        <property type="term" value="P:triglyceride biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045034">
        <property type="entry name" value="O-acyltransferase_WSD1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009721">
        <property type="entry name" value="O-acyltransferase_WSD1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31650:SF52">
        <property type="entry name" value="DIACYLGLYCEROL O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31650">
        <property type="entry name" value="O-ACYLTRANSFERASE (WSD1-LIKE) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06974">
        <property type="entry name" value="WS_DGAT_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="O-acyltransferase WSD1 C-terminal" evidence="2">
        <location>
            <begin position="77"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="O-acyltransferase WSD1 C-terminal" evidence="2">
        <location>
            <begin position="224"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7728502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="276" mass="31958" checksum="1EEE867EBD8A1063" modified="2024-05-29" version="1" fragment="single">YLRGRSTKSHACLIIFWTINDVLFGIVSYGLSKYLDSRSSKPLREGLRMTGVAMVNLRPQREYQDLEEFVRDPKAGWGNKFGMLVLPTYYHRSGSDPLEYLRRAKAMIDQKKSSLESYFSYKFGYLIMSILGAKYASRLNYRILCNTTFTISNIVGPKEKLTFAGHPVDYIKTTSSSLPHNPEKFVHLEDLRNDFRKMMIPQAELVERLTWMMSWLVWLMTRIKAITMHMLSYAGTAYMQILVAKDLVHDPEVLAKCLEDAMLEMKEAALPKIKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G5V3</accession>
    <name>A0AAD5G5V3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_001096</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011193">
        <property type="protein sequence ID" value="KAI7728578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G5V3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26312:SF214">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT (TPR)-LIKE SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26312">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7728578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="230" mass="25204" checksum="33DADD1FECBC2D37" modified="2024-05-29" version="1">MNSPKKLIRASSETDLVINSLYPVIKTTASLIASIAVEEDTEGEESDHEFFMFSTSGLYEVEVDGTGGGGGEGKRLGGHGCGNGHEDDNNNNSTDLYYQNMIEANPGNAMLLSNYAKYLKEVRGDFVKAEEYCSRAILANPNDGSVLSMYADLIWETQKDAPRVQNYFDQAVQASPDDCYVMASYARFLWDADDEEEEEEVVNQDNCDMNVLTPSFLTGTSQLPPIAAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G6M2</accession>
    <name>A0AAD5G6M2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_020383</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010995">
        <property type="protein sequence ID" value="KAI7729476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G6M2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45376">
        <property type="entry name" value="CHAPERONE DNAJ-DOMAIN SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45376:SF1">
        <property type="entry name" value="CHAPERONE DNAJ-DOMAIN SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="2">
        <location>
            <begin position="83"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7729476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="148" mass="16679" checksum="BF588942572295F3" modified="2024-05-29" version="1">MNRAATLALLNLNNSPYYIKAALFHAGRFYRSSSWGWHEHEREPSLSHGSSRIITNSGFNNRVQKNDDDKDNTSEKVETDLRNERIALGLSGSGPLSLEDVKNAYRACALQWHPDHHNDSSKAIAAEKFKVCNAAYQSLCDKLMTNKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G720</accession>
    <name>A0AAD5G720_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">AP2/ERF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_005765</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the AP2/ERF transcription factor family. AP2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010584">
        <property type="protein sequence ID" value="KAI7730959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G720"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00018">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.730.10:FF:000004">
        <property type="entry name" value="AP2-like ethylene-responsive transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.730.10">
        <property type="entry name" value="AP2/ERF domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001471">
        <property type="entry name" value="AP2/ERF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036955">
        <property type="entry name" value="AP2/ERF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32467">
        <property type="entry name" value="AP2-LIKE ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32467:SF118">
        <property type="entry name" value="ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR RAP2-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00847">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00367">
        <property type="entry name" value="ETHRSPELEMNT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00380">
        <property type="entry name" value="AP2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51032">
        <property type="entry name" value="AP2_ERF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AP2/ERF" evidence="9">
        <location>
            <begin position="47"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="AP2/ERF" evidence="9">
        <location>
            <begin position="139"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="21"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="21"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7730959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="379" mass="41728" checksum="8E50077ED0FA585F" modified="2024-05-29" version="1">MLCPDSNELREMRSVQLQQQVQVQQQKPNVQPVGKKSRRGPRSRSSQYRGVTFYRRTGRWESHIWDCGKQVYLGGFDTAHAAARAYDRAAIKFRGVDADINFSISDYEEDMKQMPNFSKEEFVHILRRQSTGFARGSSKYRGVTLHKCGRWEARMGQFLGKKYMYLGLFDSEVDAARAYDKAAIECNGREAVTNFEAHTYEGNMLPTSHHGADHNLNLSLGISTGPSHGAGSSGNETLDQAHMKYMHDTRRLQLENPVSSTVGMTALKGLPISSGHTPSWGGVYPTIVPNYQGSATNSKTISVSPQGPPFGWQIHGLNHIPAAAASSGFSAPAATSGSHQSRDTILIAPLNIHFSPSTAATSSSQYYYQIRPPPPPPPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G792</accession>
    <name>A0AAD5G792_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Triosephosphate isomerase, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_007952</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="4">Carbohydrate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the triosephosphate isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010408">
        <property type="protein sequence ID" value="KAI7731665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G792"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004807">
        <property type="term" value="F:triose-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046166">
        <property type="term" value="P:glyceraldehyde-3-phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019563">
        <property type="term" value="P:glycerol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00311">
        <property type="entry name" value="TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000025">
        <property type="entry name" value="Triosephosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00147_B">
        <property type="entry name" value="TIM_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035990">
        <property type="entry name" value="TIM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022896">
        <property type="entry name" value="TrioseP_Isoase_bac/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000652">
        <property type="entry name" value="Triosephosphate_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020861">
        <property type="entry name" value="Triosephosphate_isomerase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00419">
        <property type="entry name" value="tim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21139">
        <property type="entry name" value="TRIOSEPHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21139:SF2">
        <property type="entry name" value="TRIOSEPHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00121">
        <property type="entry name" value="TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51351">
        <property type="entry name" value="Triosephosphate isomerase (TIM)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00171">
        <property type="entry name" value="TIM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51440">
        <property type="entry name" value="TIM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="11"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7731665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="297" mass="31505" checksum="152AB025455B5B54" modified="2024-05-29" version="1">MAAMVSTYLTPSSKPSAAASPPQFAGLRRSLSTSHSFFHSVNSHTLRSSNRPTRGIVAMAGSGKFFVGGNWKCNGTKDSIKQLVSDLNSATLEPDVDVVVGPPFVYIDQVKNSLTDRIEIAAQNSWIGKGGAFTGEISAEQLKDIGCKWVILGHSERRHVIGEDDQFIGKKAAYALGQDLGVIACIGELLEEREAGKTWDNIVIAYEPVWAIGTGKVASPEQAQEVHVAVRDWLAKNVSAEVASKTRIIYGGSVNAGNSADLAKKEDIDGFLVGGASLKGSDFATIINSVTAKKVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G8E8</accession>
    <name>A0AAD5G8E8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Replication protein A subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">M8C21_028010</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">AA19_3_7</strain>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Component of the replication protein A complex (RPA) required for DNA recombination, repair and replication. The activity of RPA is mediated by single-stranded DNA binding and protein interactions. Probably involved in repair of double-strand DNA breaks (DSBs) induced by genotoxic stresses.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Heterotrimer of RPA1, RPA2 and RPA3 (canonical replication protein A complex).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the replication factor A protein 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010262">
        <property type="protein sequence ID" value="KAI7732297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G8E8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04474">
        <property type="entry name" value="RPA1_DBD_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04475">
        <property type="entry name" value="RPA1_DBD_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04476">
        <property type="entry name" value="RPA1_DBD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000041">
        <property type="entry name" value="Replication protein A subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000064">
        <property type="entry name" value="Replication protein A subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000090">
        <property type="entry name" value="Replication protein A subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047192">
        <property type="entry name" value="Euk_RPA1_DBD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013955">
        <property type="entry name" value="Rep_factor-A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007199">
        <property type="entry name" value="Rep_factor-A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031657">
        <property type="entry name" value="REPA_OB_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004591">
        <property type="entry name" value="Rfa1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003871">
        <property type="entry name" value="RFA1B/D_OB_1st"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00617">
        <property type="entry name" value="rpa1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47165">
        <property type="entry name" value="OS03G0429900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47165:SF4">
        <property type="entry name" value="OS03G0429900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02721">
        <property type="entry name" value="DUF223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04057">
        <property type="entry name" value="Rep-A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08646">
        <property type="entry name" value="Rep_fac-A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16900">
        <property type="entry name" value="REPA_OB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3 12" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Replication factor-A protein 1 N-terminal" evidence="14">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="Replication protein A 70 kDa DNA-binding subunit B/D first OB fold" evidence="13">
        <location>
            <begin position="102"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="Replication protein A OB" evidence="16">
        <location>
            <begin position="232"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="Replication factor A C-terminal" evidence="15">
        <location>
            <begin position="392"/>
            <end position="537"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU364130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF04057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF08646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF16900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAI7732297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="546" mass="61201" checksum="F951D36F3A7B149B" modified="2024-05-29" version="1">MLQSSLASEVINGNIQNLGLIRVVDYTLNDIPNKNEKYLIVTKCEAVSPPLEAEVKVEVKTEGAGILLKPKQEVVTKSAAQIIHEQNGNMAPSARMSMTRRVHPLASLNPYQGNWTIKVRLTSKGNMRHYKNARGEGCVFNVELTDEDGTQIQATMFNEAAKKFFDMFEMGKVYYISKGTLKVANKQFKTVQNDYEMTLNEYSQVEEAVNEAAFIPETKFNFVPIDQLGPYVNQKELIDVIGVVQSVSPTMSIRRKIDNESIPKRDITIADDTKKTVVISLWNTLATDLGQKLLDMAGESPVVAIKSLRVSDFQGVSLSTVNKSIIEINPNSPDSEKLKSWFQTEGKETNMESIGSNLSPSMKNGGRSMYTDRVTLDQITSNSSLGEDKPVWYSVRACMSMIRSDQAMYYQACKTCLKKVTEDMGSGYWCQACQKNESDCSLRYILSAKFTDTSSGAWFSIFSDEAEKLIGCSASELHNLKSQDDSNDFQTQLKKATWVPYCFRVMVAQKEYNNEKRQRITVKSIAPVDFAAESKMLLEELSSMKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GAM9</accession>
    <name>A0AAD5GAM9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_008800</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009631">
        <property type="protein sequence ID" value="KAI7734862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GAM9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053234">
        <property type="entry name" value="RPM1_Interactor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33443:SF31">
        <property type="entry name" value="RPM1 INTERACTING PROTEIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33443">
        <property type="entry name" value="ZGC:112980"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="270"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="318"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="271"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="325"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="370"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7734862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="500" mass="55282" checksum="13C7D35404D96C60" modified="2024-05-29" version="1">MDPKSAVLEISSDEEVDDHKWITELLGDDSDDVVVVNEFDDDKVKDFKKQNLKSLLVNNNNNDDVFYDDECILLEKDPNAPVEVLDVSANNGDDDDIVVVSEKGQVACRDYPHSRHLCIKFPFSSTPNQSHCNQCYCYVCDSLAPCVYWGNGSTSTDHCHATDKEEFWKHERKNARKGGKPVQPVTQLTPLAMLRSQNQIPRPGPILANHTSATFQYPGVISQSRNNMLASRNRLHPNLVSRLGHRAQQRSNTGYRVHRPVFKRPHPVRAATTTNQIPYSSYRGNIGNPIRQPNYQVNGMLSGSSKYLGSLQPNAVNAPVPYPPSQTYTSPNVNQPQPQFSYNPNPNFQSHVNSNPISETPLYPQVRNAPPSSSRLQPASSQQTYTVPVQPSYHVPSQTQVGSPLFPVSDFAHNTLQGNQSQGHVVDSGFKDYGLGLPTGQDSATVEGVGVTNESSLAAGSGGLADYQFDWIFDNQPIEPGFTDYSSDSAFIDTGPIFQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GAS4</accession>
    <name>A0AAD5GAS4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Actin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_018963</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the actin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009685">
        <property type="protein sequence ID" value="KAI7734619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GAS4"/>
    <dbReference type="SMR" id="A0AAD5GAS4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048767">
        <property type="term" value="P:root hair elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd10224">
        <property type="entry name" value="ASKHA_NBD_actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000291">
        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000001">
        <property type="entry name" value="Actin, muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000404">
        <property type="entry name" value="Major actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000058">
        <property type="entry name" value="Putative actin-related protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020902">
        <property type="entry name" value="Actin/actin-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004001">
        <property type="entry name" value="Actin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00190">
        <property type="entry name" value="ACTIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00406">
        <property type="entry name" value="ACTINS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00432">
        <property type="entry name" value="ACTINS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01132">
        <property type="entry name" value="ACTINS_ACT_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7734619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="377" mass="41712" checksum="2478DE523F80283D" modified="2024-05-29" version="1">MADAEDIQPLVCDNGTGMVKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDAYVGDEAQSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDSLMKILTERGYMFTTTAEREIVRDMKEKLAYVALDYEQELETAKSSSSVEKNYELPDGQVITIGAERFRCPEVLFQPSLIGMEAAGIHETTYNSIMKCDVDIRKDLYGNIVLSGGSTMFPGIADRMSKEITALAPSSMKIKVVAPPERKYSVWIGGSILASLSTFQQMWISKGEYDESGPSIVHRKCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GAV3</accession>
    <name>A0AAD5GAV3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">GINS subunit domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_032113</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GINS3/PSF3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009661">
        <property type="protein sequence ID" value="KAI7734694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GAV3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000811">
        <property type="term" value="C:GINS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902975">
        <property type="term" value="P:mitotic DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11713">
        <property type="entry name" value="GINS_A_psf3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21693">
        <property type="entry name" value="GINS_B_Psf3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2050">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021151">
        <property type="entry name" value="GINS_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036224">
        <property type="entry name" value="GINS_bundle-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010492">
        <property type="entry name" value="GINS_Psf3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038437">
        <property type="entry name" value="GINS_Psf3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055221">
        <property type="entry name" value="PSF3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22768">
        <property type="entry name" value="DNA REPLICATION COMPLEX GINS PROTEIN PSF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22768:SF0">
        <property type="entry name" value="DNA REPLICATION COMPLEX GINS PROTEIN PSF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22466">
        <property type="entry name" value="PSF3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05916">
        <property type="entry name" value="Sld5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158573">
        <property type="entry name" value="GINS helical bundle-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160059">
        <property type="entry name" value="PriA/YqbF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA replication complex GINS protein PSF3 N-terminal" evidence="6">
        <location>
            <begin position="5"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="domain" description="GINS subunit" evidence="5">
        <location>
            <begin position="69"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF05916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF22466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7734694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="186" mass="20623" checksum="24AEAE7B9D6100C3" modified="2024-05-29" version="1">MARYYDIDDILADEELVPAVFLEAVNGVGLLESNDTNRVEPRSKVELPFWLASELHLRQAASVSVPPCFNKKTREEIGADGAHVDLRSRCSYFYQLGCKIVQLIGDKTIGSLLLVAFQTRYKEVLIKAHTSASALAPKFLSLLTKEETKLYAAATSSTAAFKTWRMGGPRFQKASVLGRKRKPTGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GDI1</accession>
    <name>A0AAD5GDI1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PNPLA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_011846</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the patatin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009272">
        <property type="protein sequence ID" value="KAI7736451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GDI1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047372">
        <property type="term" value="F:monoacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004620">
        <property type="term" value="F:phospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32176:SF80">
        <property type="entry name" value="ACYL TRANSFERASE_ACYL HYDROLASE_LYSOPHOSPHOLIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32176">
        <property type="entry name" value="XYLOSE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PNPLA" evidence="4">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DGA/G" evidence="3">
        <location>
            <begin position="89"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7736451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="166" mass="17906" checksum="3E1EDC1513B0A6D1" modified="2024-05-29" version="1" fragment="single">MLTTPNEEGRPAYAAKDVKEFYLEHCPKIFPHENHPFAGATNLKNIPSLDAKISDICIGTSAAPTYLPSHSFQTEDSEGKSLREFNLIDGAVVANNPTLAAISEVSKEITTGSPDFFPIKPLEYGRFLVLSLGTGSQKFQEKYDATKSSSWGVLGWLAGGGSTPLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GEV6</accession>
    <name>A0AAD5GEV6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">BRO1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_002042</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008700">
        <property type="protein sequence ID" value="KAI7738879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GEV6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.280">
        <property type="entry name" value="alix/aip1 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004328">
        <property type="entry name" value="BRO1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038499">
        <property type="entry name" value="BRO1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23030">
        <property type="entry name" value="PCD6 INTERACTING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23030:SF30">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE NON-RECEPTOR TYPE 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03097">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01041">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51180">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BRO1" evidence="1">
        <location>
            <begin position="15"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7738879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="234" mass="25748" checksum="577C4089F70B5F8D" modified="2024-05-29" version="1">MSSPSSSSSSTTTNIMLAIYEKKTTTTDLYRPLRNYITFNYSEHEAQTLDDDLQTLNQMRTTIERSSPIDSLTSRRDLLQNYFKTLTLVESRFPISNNKEDINTVTFTWYDAFKSKQKAVQQNVHLEKAAVLFNLGAVYSQIGLGFDRMSVEGRREAVKAFVAAAGAFAFLKESEAGKASGGDLKTTVDVSVECAGMLERLMLAQAQECVFENSIAKGSSSGVCSKIARQVGAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GF35</accession>
    <name>A0AAD5GF35_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_022129</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010008527">
        <property type="protein sequence ID" value="KAI7740067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GF35"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000030">
        <property type="entry name" value="Ethylene receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.130:FF:000025">
        <property type="entry name" value="Histidine kinase 1-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050736">
        <property type="entry name" value="Sensor_HK_Regulatory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43711:SF18">
        <property type="entry name" value="HISTIDINE KINASE RESPONSE REGULATOR AND TRANSCRIPTION FACTOR RR-A-TYPE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43711">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="52"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="426"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="10">
        <location>
            <begin position="485"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="11">
        <location>
            <begin position="1005"/>
            <end position="1136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="923"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="925"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="7">
        <location>
            <position position="1067"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7740067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="1140" mass="128032" checksum="02EDF3DDD13F3F65" modified="2024-05-29" version="1">MITPVAMASFVRRMLDRISASVSSSGRNHLPTRLRRSRSDVNQDEFRCYSVFVARVAIMVMLAVLIGLLTLLTWHFTKVYTRASLNSLAYGLRYELLQRPLLRMWKILNSTVEITTSQVRLSEYVIRKYGKGENQAQQVELYEVMRDVTWALFSSRKALNSMSIKYRNGFVQAFHRDLRDKNTYYIYSNLYNYTMVEPYSMNRSSTHQGWNDQMIHGNVSAIWYREPLDPDTGRKTGKQKEIPPDELINIAGISQVPDGAASWHVSVSKFTSSPLLSAALPVWDEDRGSIVAVVGVTTALYSVGQLMKELVDLHGGHIYLTSQKGWLLATSSSTPLLRNSSDGYTLTMAEDSQDGIIRSAAKWLNKTNHNKLPLNHEVHSATATLGGERYYIDSFFLNLTRLPMVGVLCIPRKNIMGKVDAREIKTLIILISASVGILVVGCICIFILTNGVSKEMKLRAQLISHLDARRKAEASSNFKSQFLANMSHELRTPMAAVIGLLDILLSDDSLTNEQYATISQIRRCSTALLRLLNNILDISKVESGKLVLEEAEYNLGKELEGLVDMFSVQCKNRNVEAIIDLSDDMPKVVRGDSGRVVQIFANLISNSIKFTTSGYIVLRGWCENLNSFSNNENFFVDHKALWSAYQKKIKRKTCKSDNIMILFFEVEDTGCGIDPSKWESVFESFEQADPSTTRLHGGTGLGLCIVRTLVNKMGGEIRVVKKNGSGTLMQLYLLISTPADIPKESYRLKFAEHNLKVVLALNGRMSGSILSRWLHKLGVHIWEANDWNQLTHVLQELFKPTSYAQSLTYERSETESLRTEGVKMTIFVIVIDIGMLNISTDIWKEQLNFLDAFNTRAKFAWILNHDTSNTIKIELRRRGHSLMVNGPLYKTKMIQIIEAVIKESSLEQSKKYIDAHECLQTNAKSSDGSEITETTSTGSSQNEETNQENFQVYGVVSNCFPELTEGRSEWGRLTNFPNDSSNCEQGIHQADQKVKNGKKALTGLHILLAEDTPILQKVATMMLVQMGAKVVVVGDGVQAVEALNIQKEVENVEKSCEPRPFDLILMDCQMPKMDGYEATKAIRRAEMESESHIPIVALTAHAMSSDEAKCLEVGMDAYLTKPIDCKLMASTILSLTKCRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GFE9</accession>
    <name>A0AAD5GFE9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_008434</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008822">
        <property type="protein sequence ID" value="KAI7738371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GFE9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="82"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="107"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7738371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="128" mass="13834" checksum="FDA9B07C75354D4B" modified="2024-05-29" version="1" fragment="single">PRKTETIVYAFTAASKMVVVLSAMLVVAALVPAVQDSRNFFMRVGTPPPPTPFTLCGEVKMKESGQTSLQLPSINEQITLSHQQQQVNIPIASHANSGPTPSPTPNPNTRRLTPNLPCRDLLTGNFDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GKI6</accession>
    <name>A0AAD5GKI6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_013822</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007544">
        <property type="protein sequence ID" value="KAI7744369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GKI6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37248">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37248:SF1">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7744369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="237" mass="26112" checksum="F1B9EC3EC346779D" modified="2024-05-29" version="1">MPKRKAKKVTKPPASSQLNDSQCNADTRVLPEGNAFIDQEVERQSAAIRAIRDVETERLLTAIRLLKSSISKEQLQTPLLQFFEENLPNLTISRTEKNGPIEVKWKNKGADISMTTADGGNMISYFLQHMANPEFSAAIPSLGGFDISSQAGRTSFIGADNLQMKDFVLEEPSDTHILGIEDYMQTPGVTSGRLSVGMTPKTLSLPKQGEMLLSVHGSPLGVYKENNMDAIRETEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GM75</accession>
    <name>A0AAD5GM75_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_017259</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007051">
        <property type="protein sequence ID" value="KAI7746049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GM75"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7746049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="72" mass="8245" checksum="186E398D2AFA2180" modified="2024-05-29" version="1" fragment="single">INCCRLSDDADLIYGLIWDELVSSCIEIGTLDTMGISMRNAVAQSRIQKFDQRIHYPYQYKETIDGRSTLAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GMG1</accession>
    <name>A0AAD5GMG1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein DETOXIFICATION</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Multidrug and toxic compound extrusion protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_007580</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006526">
        <property type="protein sequence ID" value="KAI7748422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GMG1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13132">
        <property type="entry name" value="MATE_eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045069">
        <property type="entry name" value="MATE_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11206">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="64"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="97"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="124"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="169"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="213"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="315"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="359"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="390"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="418"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7748422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="446" mass="49138" checksum="6ABDD2A19FF8E029" modified="2024-05-29" version="1" fragment="single">LGMSSATETLCGQAFGAGQHHMMGIYLQRSWIVDLVTLTILLPIFIFATQIFKLLGQDEAVANSGGYISLWFIPFVYNYVFSLTIQMYLQAQLKNMVIAWLSIFRFGIHILLSLLLVYKLNLGVVGAMIALSMSSWFLVIGEFVYIFGGWCPNSWKGFTVAAFKDLVPVVKLSISSGVMLCLELWYYALLVLLAGYMADAEVAISAFSISLNIYAWEFMISLEFVYIFGGWCPYSWKGFTFAAFKDLIPVVKLSISSGVMVCLELWYDAVLVLLAGYMADAEVAISAFSICLNINTWDVRVANELGRGNAKAAKFSIKVLLGTSIAIGVFFFVLSLIFGEKIAYLFTNDDRVADTISDLSLLLSFSILLNSIYPVLSGVAVGAGMQGTAAIVNLVCFYLIGIPIGALLGYLTNLQVKGIWMGMIGGFVTETIALIYMVWAINWDEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GNC9</accession>
    <name>A0AAD5GNC9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_019367</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006776">
        <property type="protein sequence ID" value="KAI7747339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GNC9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7747339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="156" mass="17131" checksum="70F200463787D346" modified="2024-05-29" version="1">MMGSVSLVGLKRSIRVGVGRWKRKKSVVRGSVVRLNVVKCLGVGKGVMKGNVTIIRCRGGERVHVGNGFMKGWGVMLWCRLVVGRVIRSCGVGFIGVRGEDVIGFIYNRFLVIKMKYVKVNAKECGIVDAKHVNENVVMVLLVQSNAEEEDTAGRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GNE2</accession>
    <name>A0AAD5GNE2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Fiber protein Fb34</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_001705</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the DESIGUAL family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006934">
        <property type="protein sequence ID" value="KAI7746621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GNE2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009606">
        <property type="entry name" value="DEAL/Modifying_wall_lignin1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052222">
        <property type="entry name" value="DESIGUAL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31769">
        <property type="entry name" value="OS07G0462200 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06749">
        <property type="entry name" value="DUF1218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0732">Signal</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041896908" description="Fiber protein Fb34" evidence="8">
        <location>
            <begin position="22"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="48"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="87"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="138"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7746621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="181" mass="19493" checksum="AC5A78CD51F73286" modified="2024-05-29" version="1">MSSTLLLAVVFVLDLVAFALAVAAEQRRASARIAQDARSRYCVYESDIATGLGVGALLFLLVSQLLVMAATRCLCCGRVLIPGRSRALAIFLFITCWVTFIIAEACLLAGSVRNAYHTEYRTVLSANPPSCETLRKGMFGAGAAFVVLTGIASELYYVFYSKSDEGVIPTRDTGIRMGAFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-04-02" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GPW3</accession>
    <name>A0AAD5GPW3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_024608</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006717">
        <property type="protein sequence ID" value="KAI7747613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008194">
        <property type="term" value="F:UDP-glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48045:SF35">
        <property type="entry name" value="UDP-GLUCURONOSYL_UDP-GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48045">
        <property type="entry name" value="UDP-GLYCOSYLTRANSFERASE 72B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7747613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="147" mass="16805" checksum="82A60DEF978C2BBA" modified="2024-05-29" version="1">MDAKVLTEMAWGLMKSDQRFLWAVRPGLVSGSEWLELLPEGFIEKTKERGLIVKWAPQKDVLAHFAVGAFWSHCGWNSCLESISLGVVMICQPFDVDQWVNARYVSFVWKIGIELEQVERGEIERXXXXXXXXXXXXXXXXXXXXXX</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GRV4</accession>
    <name>A0AAD5GRV4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RNA polymerase sigma-70 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_031840</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006173">
        <property type="protein sequence ID" value="KAI7750266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GRV4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071482">
        <property type="term" value="P:cellular response to light stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.601.10">
        <property type="entry name" value="RNA Polymerase Primary Sigma Factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000943">
        <property type="entry name" value="RNA_pol_sigma70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007624">
        <property type="entry name" value="RNA_pol_sigma70_r3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050239">
        <property type="entry name" value="Sigma-70_RNA_pol_init_factors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30603">
        <property type="entry name" value="RNA POLYMERASE SIGMA FACTOR RPO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30603:SF52">
        <property type="entry name" value="SIGMA FACTOR A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04539">
        <property type="entry name" value="Sigma70_r3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00046">
        <property type="entry name" value="SIGMA70FCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="Sigma2 domain of RNA polymerase sigma factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="Sigma3 and sigma4 domains of RNA polymerase sigma factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00715">
        <property type="entry name" value="SIGMA70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0731">Sigma factor</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="RNA polymerase sigma-70" evidence="6">
        <location>
            <begin position="291"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7750266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="531" mass="59611" checksum="0F95D2CA2B284355" modified="2024-05-29" version="1">MMATNAVIGLRAGERLLTPSSYYSDVNEKLSSGNELGFVAVPTKNVIAAKKSTNYGHGFIPKHHNQDTHYIRALKVTTASGFSSIDQWVHGFEHLVHEDADQEFSVEALLSLQKSLLEKQWNLSTETTLTKVKLSEKSSKKIYVNGSGKSARRRRIDAQRKIPNRRLSSNEVGLKKQLKFVISPELLQNRASGYVKGITSDTLLTHAEVVILSKKIKIGQRLEERKSRLKESLGSEPSEVQLAASLKISRAELQMKQIECKLAREKLAMSNVRLVMSVAQKYANMGAEIGDLIQGGLIGLLRGIEKYDSSRGFKISTYVYWWIRQGVSKALIENSRTLRVPVHVHERLSAIRHAKAKLEKKGITPSIEKIAESLNMSTKKVLNATEANCKVFSLDRPAFPSLNGLSSDTFHSYMADDHPENNPWHGVDNGALKDEVNKLMTTSLREREREIIRLYYGLDNEFLTWEDISRRLRVTGQNGYRLNGSLLIASLNTLSSMGLSRERVRQVGLVALEKLKHAARKTNLDAMLVEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-06-18" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GUA5</accession>
    <name>A0AAD5GUA5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PWWP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_031674</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010001074">
        <property type="protein sequence ID" value="KAI7755500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006569">
        <property type="entry name" value="CID_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000313">
        <property type="entry name" value="PWWP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12550">
        <property type="entry name" value="HEPATOMA-DERIVED GROWTH FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12550:SF49">
        <property type="entry name" value="PROTEIN HUA2-LIKE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00855">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00293">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00582">
        <property type="entry name" value="RPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50812">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PWWP" evidence="3">
        <location>
            <begin position="24"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="129"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="227"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="450"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="531"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="612"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="837"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="912"/>
            <end position="934"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="983"/>
            <end position="1113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="129"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="246"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="461"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="477"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="497"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="542"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="845"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="916"/>
            <end position="934"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="984"/>
            <end position="1063"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1066"/>
            <end position="1080"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1100"/>
            <end position="1113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7755500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="1287" mass="139569" checksum="B3221E1469E1F29E" modified="2024-05-29" version="1">MAPSRRRGAGKAAAVAAACRKWKVGDLVLAKMKGFPAWPAMVSEPEKWGYSVDWKKVLVFFFGTQQIAFCNPADVEAFTEEKKVSLLNKCHGKGADFRRAVREIIDSYEELKKHEQDNNANDIIGQVASTNGIKSEVPSSGSKDEADTPTVDSCPKISDSLEPNSEGAALLVQDDKITSEWHNGNLAVKKAPLPTTYSRKKYSGTQVPSARRLRSLVREDAVLNNGNSLSLSRDVPQKRTKRVGTSPGSSDGLGSNASPEENGSEIVTVDSDTKNLNEGNCVQSDYNKVMENEYVKQLSQTLKFQNKAVITKKKRKPSRKREFCDKVDKINKTESTQNSVIEETIGKYSKEDGDEHLPLVKRARVRMGRTIETVSRPSHDCGVSIDAEDTARKGLEPCSTLHNCSVNKLPSQEANNNNNHFGCFVDDEAALPPSKRLHRALEAMSAYVAEDKQGSPGGPSTMKTVINGNLESKGADDSSSNLSSPVEENETIDGIDTCSQPSTTSSPEQKPIEVADVKDYVMLDSFTEAVETVAGPRSPKSSSEDIEKKESSPELKKDPVSSNLENGIVSHDDVTLLPSPPQKSCSIVDHPVQDAANDNAIFCNRVEDDEETKKSMMEVDDSPSHTSVKELTVDPDSELLNSTHISEDDMRHKSVSGIRTPSLLTEPLDSADVSRASPPNNTSICLTTSTSYNSNLLENSGCSSPNVQEKAEHNSNKWSTISEANAALASFEASLGALSRTKKNIDRATRIAIDCAKFGIAVKVVETIVRSLEAQSSLHKRVDLFFLVDSIAQCSRGLRGDVGGLYPSALQAMLPRLLLAAAPPGSSALENRFEAVARKKDPSRTNDPSPYSINNSHRPFRNERAFDDPVRDVEGMLVDEYGSNSSMQLSGFCMPTMLKDEKEESDSDAEGFEAVTPERDPEICDEGDKVPTPSAEKHTHVLEDVDGELEMEDVAPICEGEVTVTALLAKAAEVSQLHLQQQLPPLPEDLPPSSPPLPKSPPPHASFPPPPPPPQASVPLPPPPPATLPPPPATLPPLPPLAILPPPPPPPPATLPPPPPPPVTITSLPDTLPPSSTSVSAPLSLPDTIGNVPDAKPYVASQNGNDDSQQSTDALSVTYHSSEVPESSNSCSFGVLRPPVQAASNVHLRPPHPAPSSQFSYFQSDQLCSEIPPPSYPGRFHFVSSTDTGNFHSDHDRMHVPAQDDSWRFQPPHFSGPFHPDGPRGQYPPNMYAGPHCEPPMSSNTWHYPVRPPNHRDMATHRPYPEASVHMTTRVSTLIWAKCSPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GVW6</accession>
    <name>A0AAD5GVW6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_015815</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005359">
        <property type="protein sequence ID" value="KAI7753673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GVW6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032436">
        <property type="term" value="P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09917">
        <property type="entry name" value="F-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14939">
        <property type="entry name" value="F-BOX ONLY PROTEIN 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14939:SF5">
        <property type="entry name" value="F-BOX ONLY PROTEIN 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="8"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7753673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="353" mass="38593" checksum="E5C0EFBAE37C7CB5" modified="2024-05-29" version="1" fragment="single">MASPTTIDHLGMDLLHNILARLSSIDFASADCVSRSWNHVCGRILCRPKLSSACSFNQSLQVAVEEVVNKVLSERIRPHFAIASVGTRFHIKFNIEEAHKLITAKLGSKVPLITNCSPGIIGRDVNSGEFKEASRAYGDTSITDEFDRSLVQERAKGGIMLTVGFLPGLKVKLIKLAKEDLTSQDARAYSKKFINDIREFSASISGCQSPAAIMMFSEPLQGVIDVMEKVDHAMSPETVIVGDYGYRFHSDTESLNNITATKERVSAVVALVFVMDRTKPLDPATALSSIANVSNHLRSFKQERTAAGDKREVFGGFIFTSYDRSDFRKPNVNSSPFLDNFPSATLGGILGGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GWU7</accession>
    <name>A0AAD5GWU7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Probable magnesium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_015786</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 9">Acts as a Mg(2+) transporter. Can also transport other divalent cations such as Fe(2+), Sr(2+), Ba(2+), Mn(2+) and Co(2+) but to a much less extent than Mg(2+).</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 9">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Early endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the NIPA (TC 2.A.7) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010001662">
        <property type="protein sequence ID" value="KAI7755201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GWU7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3730.20:FF:000006">
        <property type="entry name" value="Probable magnesium transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3730.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008521">
        <property type="entry name" value="Mg_trans_NIPA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12570:SF65">
        <property type="entry name" value="MAGNESIUM TRANSPORTER NIPA9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05653">
        <property type="entry name" value="Mg_trans_NIPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0967">Endosome</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="72"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="98"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="128"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="170"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="200"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="232"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="266"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7755201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="335" mass="36216" checksum="6B623BB182208651" modified="2024-05-29" version="1" fragment="single">WEAILLTVAATAGNNIGKVLQKKGTVILPPLSLKLKVVRAYASNKPWLIGFIMDIFGALLMLRALSLAPVSVIQPVSGCGLAILSVFSHFYLKEVMNVFDWMGITLAGVGTIGVGAGGEEQKASSISILHLPLLACGVAFLFVLLNGWLRIYRRQRREQELMQSEVVEEIIYGLESGILFGIASVISKMGFLFLEQGFSALLVPVCISISICCSASGLVYQTRGLKHGRAVVVSTCAAVASIVTGVLAGMLALGERLPESPASRSWLLLGWLLIVTGVMLLVTSTRLIRFLPRKWRPLERRQPNSLRSREPGSNPSTVIQATTLHHLVTSSKAKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GXG8</accession>
    <name>A0AAD5GXG8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Alpha,alpha-trehalose-phosphate synthase (UDP-forming)</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_004031</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the N-terminal section; belongs to the glycosyltransferase 20 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000817">
        <property type="protein sequence ID" value="KAI7755786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GXG8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003825">
        <property type="term" value="F:alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004805">
        <property type="term" value="F:trehalose-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005992">
        <property type="term" value="P:trehalose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03788">
        <property type="entry name" value="GT20_TPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01627">
        <property type="entry name" value="HAD_TPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000100">
        <property type="entry name" value="Alpha,alpha-trehalose-phosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000039">
        <property type="entry name" value="alpha,alpha-trehalose-phosphate synthase [UDP-forming] 1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001830">
        <property type="entry name" value="Glyco_trans_20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003337">
        <property type="entry name" value="Trehalose_PPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788:SF130">
        <property type="entry name" value="ALPHA,ALPHA-TREHALOSE-PHOSPHATE SYNTHASE [UDP-FORMING] 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788">
        <property type="entry name" value="TREHALOSE-6-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00982">
        <property type="entry name" value="Glyco_transf_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02358">
        <property type="entry name" value="Trehalose_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="556"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="561"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="596"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="664"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7755786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="664" mass="74550" checksum="474B8FD7CEE818DA" modified="2024-05-29" version="1" fragment="single">FPSSEIHRTLPSRSELLRAVLAADLVGFHTYDYARHFVSACTRILGLEGTPEGVEDQGRLTRVAAFPIGIDSDRFIRALENPLVQEHMKELKERFSGRKVMLGVDRLDMIKGIPQKILAFEKFLEENPYWRDKVVLLQIAVPTRTDVPEYQKLTSQVHEIVGRINGRFGTLTTVPIHHLDRSLDFQALCALYAVTDIAIVTSLRDGMNLVSYEFVACQDDKRGVLILSEFAGAAQSLGAGAILVNPWNITEVAASIGQALNMSAEEREKRHRHNFLHVTTHTAQEWAETFVSELNDTVVEAQQRIRQVPPPLPVEEAIERYLQSSNRLIILGFSATLTEPVDTPDRRGGDQIREMDLKLHPELKESLTKLCDDPHTTVVVLSGSDRTVLDDNFVEFNMWLAAENGMFLRSTKGTWMTTMPEHSNMEWVDSVKHVFEYFTERTPRSHFELRETSLVWNYKYADVEFGKLQARDMLQHLWTGPISNASVDVVQGSRSVEVRAAGVTKGITIVRILGEIVHSKSISSPIDYVLCIGHFLGKDEDIYTFFEPELPYNGMGIPRPKLNDPAKGPGDKRPGKSGSKSSQKARAPQSPDNKRTTNNNRNNGFENGGKRSSVSADKVSWNVLDLKADNYFSCAVGRTRTNARFVIPSSDEVVTFLKELTQAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GYG3</accession>
    <name>A0AAD5GYG3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AIG1-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_032414</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. AIG1/Toc34/Toc159-like paraseptin GTPase family. IAN subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000523">
        <property type="protein sequence ID" value="KAI7756208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GYG3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000840">
        <property type="entry name" value="Immune-associated nucleotide-binding protein 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006703">
        <property type="entry name" value="G_AIG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045058">
        <property type="entry name" value="GIMA/IAN/Toc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903:SF184">
        <property type="entry name" value="GTP-BINDING PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903">
        <property type="entry name" value="GTPASE, IMAP FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04548">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51720">
        <property type="entry name" value="G_AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIG1-type G" evidence="4">
        <location>
            <begin position="15"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7756208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="336" mass="38094" checksum="7EE68B340A275903" modified="2024-05-29" version="1" fragment="single">MSMEGSLFEGDREFSFAQTLLLVGRKGTGKSATGNTILGTNLFRSERSSPGVTSTCELRSSKLKDGRMINVIDTPGIDINHGILGSSVDTEFSEEVINMAWDGIHAILVVLSVCSRFSKEEEAVISRLLSVFGSKVYDYMILVFTGGDQLDKDDETLESFLHDCPEKLKELLNLCDTRCVLFDNKTKDEVKRSDQVEKLFSIVNMVSRKNGGKLYTNEIFADWKKEAKELKEQAENSQALKEFKPYTKLEVSMQTDPMHDGAFKRIIEMVEPKLKERALRLERQLAEERAARLKAEEDATVAQKKSDEEIRYINKQIETAMMKRRRALRNPTGIEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BKR2</accession>
    <name>A0AAD5BKR2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_014233</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012062">
        <property type="protein sequence ID" value="KAI7725090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BKR2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.34.10:FF:000002">
        <property type="entry name" value="Heat shock 70 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.10:FF:000015">
        <property type="entry name" value="Luminal-binding protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="HEAT SHOCK PROTEIN 70KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="233"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="136"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="246"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7725090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="256" mass="28530" checksum="57D34EAB72C7A445" modified="2024-05-29" version="1">MLMLPTCSQADILLLDVAPLTLGIETVGGVMTKLIPRNTVIPTKKSQVFTTYQDQQTVVSIKVYEGERSLTKDCRLLGTFDLTGIPPAPRGTPQIEVTFEVDANGILNVKAEDKASGKSEKITITNEKGRLSQEEIERMVREAEEFAEEDKKVKEKIDARNALETYVYNMKNQINDKDKLADKLESDEKEKIETATKEALEWLDDNQSAEKEEYDEKLKEVEAVCNPIVTAVYQRSGGAPGGGAESTEDEDEHDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BL06</accession>
    <name>A0AAD5BL06_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_025416</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012015">
        <property type="protein sequence ID" value="KAI7725191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BL06"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049500">
        <property type="entry name" value="Peptidase_M50B-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33979">
        <property type="entry name" value="OS02G0221600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33979:SF7">
        <property type="entry name" value="PEPTIDASE M50B-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13398">
        <property type="entry name" value="Peptidase_M50B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="109"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="140"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="164"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="190"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7725191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="244" mass="27047" checksum="E8105F1DC6A15DEB" modified="2024-05-29" version="1">MANWELRNCCNHDQLVFLITLAFCVIVILALWRTIVLLPFKLVTIFLHEASHAVACKLTCGHVEGMQIFADEGGMTQTRGGVYWFILPAGYLGSSFWGMVLILASTNLIAARIAAGCLAAALIIVLFVAKNILYKILDDLTNSILYKSVSSPLIFTLVSCSQWTLRGLCIGFIVVLAVVWVLQETTKVRILRYIIMFIGVMNSVFSIYDIYGDLISRQVHTSDAEKFAEVCPCPCNGVGWGVIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BLF8</accession>
    <name>A0AAD5BLF8_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_018180</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012193">
        <property type="protein sequence ID" value="KAI7724551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BLF8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011498">
        <property type="entry name" value="Kelch_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46422">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE BSL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46422:SF4">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE BSL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07646">
        <property type="entry name" value="Kelch_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7724551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="121" mass="12601" checksum="ACDD25A727B306FF" modified="2024-05-29" version="1" fragment="single">PLASAYGLAKHRDGRWEWAIAPGVSPSPRYQHAAVFVNARLHVSGGALGGGRMVEDSSSVAVLDTAAGVWCDTKSVVTTPRTGRYSADAAGGDAAVELTRRCRHAAAAFGDLIFMYGGLRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BNG2</accession>
    <name>A0AAD5BNG2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_005120</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011950">
        <property type="protein sequence ID" value="KAI7725404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BNG2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="134"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7725404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="162" mass="18144" checksum="0EB676B15E4E654C" modified="2024-05-29" version="1" fragment="single">VLERGVKVIKRRRHSNVVGCCSADADGFPSTFTITGCQFLFSAIFVYGYTEFALTIEIRDEAVMIKFRSSKIERCIPYPYLGLAIRMAILVPFATVMRIWAKAVASTFRSVVTVGVRFLCADHGVGNLQRPKHYLFFLSIAQITSAGITGLIKHRKFFIINA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BNM3</accession>
    <name>A0AAD5BNM3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_032481</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011550">
        <property type="protein sequence ID" value="KAI7726738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BNM3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7726738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="51" mass="5802" checksum="03E4CA9A1F420D4D" modified="2024-05-29" version="1">MGCLFFACGEVFASPTSICSRTFFNLERPTSITHKPSYVVSSSDKWDFKFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BNZ0</accession>
    <name>A0AAD5BNZ0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Ras-related protein RABE1c</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_013954</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011856">
        <property type="protein sequence ID" value="KAI7725783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BNZ0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01867">
        <property type="entry name" value="Rab8_Rab10_Rab13_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000308">
        <property type="entry name" value="ras-related protein RABE1c-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050305">
        <property type="entry name" value="Small_GTPase_Rab"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47980">
        <property type="entry name" value="LD44762P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0636">Prenylation</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="183"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="193"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7725783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="215" mass="23806" checksum="C663C719A8512CE8" modified="2024-05-29" version="1">MAAPPARARADYDYLIKLLLIGDSGVGKSCLLLRFSDGSFTTSFITTIGIDFKIRTIELDGKRIKLQIWDTAGQERFRTITTAYYRGAMGILLVYDVTDESSFNNIRNWIRNIEQHASDNVNKILVGNKADMDESKRAVPTSKGQALADEYGIKFFETSAKTNMNVEEVFFSIGRDIKQRLAETDSKAEPQTIKINQPDQSAGGSQAGQKSACCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BP28</accession>
    <name>A0AAD5BP28_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_008284</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011478">
        <property type="protein sequence ID" value="KAI7726937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BP28"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031624">
        <property type="term" value="F:ubiquitin conjugating enzyme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048437">
        <property type="term" value="P:floral organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046621">
        <property type="term" value="P:negative regulation of organ growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000226">
        <property type="entry name" value="E3 ubiquitin ligase BIG BROTHER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033276">
        <property type="entry name" value="BB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46400:SF5">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46400">
        <property type="entry name" value="RING/U-BOX SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="3">
        <location>
            <begin position="175"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="77"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7726937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="232" mass="26666" checksum="34241F38213F59A5" modified="2024-05-29" version="1">MDFFEGLTYEHVNFIFSEVTYPQDSTQSTINTNAYKFAYFEPGSFSYYDYCDAYPINNDETYGNNGYSRQLDNASTMANETTSVHTQQDGHSSSNSVADHAAADCPQSHHNPADYQIVWEDNVDPDNMTYEELLELGEAVGSQSRGLSQEAISMLPVSKFKWCFFWRKKSKNERCVICQMEYKRGDRQLTLPCKHIYHAGCGSQWLSINKACPICYKEVLVHTPKHSQRFFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BP74</accession>
    <name>A0AAD5BP74_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CRAL-TRIO domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_031882</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SFH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011474">
        <property type="protein sequence ID" value="KAI7726997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BP74"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.20">
        <property type="entry name" value="N-terminal domain of phosphatidylinositol transfer protein sec14p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011074">
        <property type="entry name" value="CRAL/TRIO_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051026">
        <property type="entry name" value="PI/PC_transfer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45657">
        <property type="entry name" value="CRAL-TRIO DOMAIN-CONTAINING PROTEIN YKL091C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45657:SF5">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL_PHOSPHATIDYLCHOLINE TRANSFER PROTEIN SFH6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03765">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01100">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46938">
        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="366"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="CRAL-TRIO" evidence="9">
        <location>
            <begin position="149"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="432"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="7"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="39"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7726997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="516" mass="58998" checksum="9AD49DB8B18D153F" modified="2024-05-29" version="1" fragment="single">FEGSSGNDERPVRRERSERRERKSDFENSEDERRTRIGSLKKKALNASSRFKHSLKKKGSRRKGDGRVSSVSIEDIRDVEELRTVDAFRQALVLDELLPEKLDDYHMMLRFLKARKFDIEKAKQMWADMINWRKDFGADTIIEDFDFHEMNEVLKYYPHGNHGVDKEGRPVYIERLGKVDPNKLMQVTTMDRYLKYHVREFEKSFAFKFPACSIAAKRHIDSSTTILDVQGVGLKNFSKSARELVMRLQKIDGDNYPEVLGNKNKLLEVIDASELPEFLGGTCTCADKGGCLLSDKGPWNNPEILQLALNSEARRTRQVVKVLNSEGEIVAYAKPQLPMVERHNFGTRILIEPAAQKGPEKFHSRIWVMLLAMFMTIFTLLSSAMSHVTKKVADSPSEVAQTAEDFSFDASTTKEECRPPSPTRLFKETEIVNSVLKRLGELEEKVDTLKAKPSEMPYEKEELLNAAVCRVDALEAELIATKKALHEALMRQEELLAYIDSQEEKKFRGSGLQNGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BPY4</accession>
    <name>A0AAD5BPY4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Vacuolar iron transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_008076</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Vacuolar Fe(2+) uptake transporter.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>Fe(2+)(in) = Fe(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:28486"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:28487"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Vacuole membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the CCC1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011435">
        <property type="protein sequence ID" value="KAI7727124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BPY4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140315">
        <property type="term" value="F:iron ion sequestering activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005381">
        <property type="term" value="F:iron ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005384">
        <property type="term" value="F:manganese ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030026">
        <property type="term" value="P:intracellular manganese ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008217">
        <property type="entry name" value="Ccc1_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31851">
        <property type="entry name" value="FE(2+)/MN(2+) TRANSPORTER PCL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01988">
        <property type="entry name" value="VIT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0410">Iron transport</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0813">Transport</keyword>
    <keyword evidence="4 9" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="88"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="115"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="145"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU369115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7727124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="169" mass="18440" checksum="21A590395D8B75C3" modified="2024-05-29" version="1">MLRMYLAAKSEADHYTRELQREQEEIDTIPDTEAAEVAEILAEYGVEPHEYGPVVNALRKNPKAWLDFMMKFELGLEKPDPKRALQSAFTIAVAYVLGGLVPLFPYMFIPVAQKAVVASVIVTILALLIFGYAKGYFTGSKPVWSALQTALIGAIASAAAFGMAKAVQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BTD2</accession>
    <name>A0AAD5BTD2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_030640</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011274">
        <property type="protein sequence ID" value="KAI7728091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BTD2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7728091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="132" mass="14508" checksum="20AEF384EF2A4E20" modified="2024-05-29" version="1" fragment="single">NDNGNLIPSFITVTCNLMVHRPPPPIYSTSSSTSSTTTSAGTLIVVKNLESDNPNTCFSNSSSNASFWTSEVDTRMIQMKLRIWCHDIATVMLEVVKTKLSMVMTAGCRQNGEQYARKIWEEMADAIGVGKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BUF4</accession>
    <name>A0AAD5BUF4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Extra-large guanine nucleotide-binding protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_010229</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the G-alpha family. XLG subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011169">
        <property type="protein sequence ID" value="KAI7728663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BUF4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005834">
        <property type="term" value="C:heterotrimeric G-protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001664">
        <property type="term" value="F:G protein-coupled receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031683">
        <property type="term" value="F:G-protein beta/gamma-subunit complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007188">
        <property type="term" value="P:adenylate cyclase-modulating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00066">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.400.10:FF:000005">
        <property type="entry name" value="Extra-large guanine nucleotide-binding protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001044">
        <property type="entry name" value="Extra-large guanine nucleotide-binding protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.400.10">
        <property type="entry name" value="GI Alpha 1, domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001019">
        <property type="entry name" value="Gprotein_alpha_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011025">
        <property type="entry name" value="GproteinA_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218:SF334">
        <property type="entry name" value="EXTRA-LARGE GUANINE NUCLEOTIDE-BINDING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218">
        <property type="entry name" value="GTP-BINDING PROTEIN ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00503">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00318">
        <property type="entry name" value="GPROTEINA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00275">
        <property type="entry name" value="G_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47895">
        <property type="entry name" value="Transducin (alpha subunit), insertion domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51882">
        <property type="entry name" value="G_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="7 11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="67"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="126"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="840"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="67"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="84"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="128"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="141"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="465"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="626"/>
            <end position="632"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="632"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="749"/>
            <end position="752"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="815"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00060880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7728663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="865" mass="98126" checksum="A92743A709102FA5" modified="2024-05-29" version="1">MRDMDEHGSKDWREAVKKMLPPGTVLPEDSDELDYSIAIEYMGPDVSYELPRVEPLDMNTRSIPTASFAESFSGSRRSVTRDGPGPPVIEPIPLPVSYIAGVTDSLNRSRRLSGSSESLSSVLHNADSYSASPSGSPGSVHNRDDANGNEVRRGPVVTFNTVDRSESREVGLERPGRSEYVAVSNGTKKKTQKRRVCYRCGKGKWESKESCLVCDAKYCSNCVLRAMGSMPEGRKCVTCLGESIDESKRLILGKNSKVLSRLLSPLEVKQIMKAEKECSANQLRPEQLIVNGYPLKPEEMAELMGCMLPPRKLKPGTYWYDKESGLWGKEGEKPDRVVSSNLSFTGKLSPSASNGDTEVYMNGREITKLELRVLKVAHVQCPRDTHFWVYDDGRYEEEGQNNIKGNIWEKASTRFLCSLLSLPVPHGQPQREKDEASNYAAVPNYFEPKKIQKLLLLGLEGSGTSTIFKQAKFLYGNRFTDEELLNIKLMIQSNMYRYLSILLDGRERFEEEALMKKNAHDQSNDTSNDGNDEETNVCVYSLNPRLKNFSDWLLDIIATGDLDAFFPAATREYAPLVEEVWKDPAIQETYKRRSELHFLPDVAEYFLSRAVEVSSNEYEPSEHDILYAEGVTQGNGLACIEFSLDDRSPMSETFTDNLDGQSQPLSRYQLIRVNAKGMNEGCRWVEMFEDVRVVVFCVALSDYDQMWLSPDNTGSGSLLQNKMIQSKELFEAMVKHPCFKDTPFVLILNKYDIFEEKLKQTPLSACEWFTDFSPVRPYTNNHNLAHQAYYYVAVKFKDLYASLTSQKLYVWQAKARDRVTVDEAFKYIREIVKWDDEKEDTYYGGPEDSFYSTTDVSSPPHVRPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BVC0</accession>
    <name>A0AAD5BVC0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cupin type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_001435</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Seed storage protein.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Hexamer; each subunit is composed of an acidic and a basic chain derived from a single precursor and linked by a disulfide bond.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the 11S seed storage protein (globulins) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010835">
        <property type="protein sequence ID" value="KAI7730147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BVC0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045735">
        <property type="term" value="F:nutrient reservoir activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02243">
        <property type="entry name" value="cupin_11S_legumin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02242">
        <property type="entry name" value="cupin_11S_legumin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000073">
        <property type="entry name" value="Glycinin G1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022379">
        <property type="entry name" value="11S_seedstore_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006044">
        <property type="entry name" value="11S_seedstore_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006045">
        <property type="entry name" value="Cupin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050253">
        <property type="entry name" value="Seed_Storage-Functional"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31189:SF54">
        <property type="entry name" value="11S GLOBULIN SEED STORAGE PROTEIN 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31189">
        <property type="entry name" value="OS03G0336100 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00190">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00439">
        <property type="entry name" value="11SGLOBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00835">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00305">
        <property type="entry name" value="11S_SEED_STORAGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0708">Seed storage protein</keyword>
    <keyword evidence="2 5" id="KW-0758">Storage protein</keyword>
    <feature type="domain" description="Cupin type-1" evidence="7">
        <location>
            <begin position="31"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="Cupin type-1" evidence="7">
        <location>
            <begin position="319"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="199"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="202"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7730147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="488" mass="54912" checksum="708E4C49C2A5F75E" modified="2024-05-29" version="1" fragment="single">TTVLATTTRSPLATTRSPRHGGPEFCNLQQLNSYQPTQRLDFEGGSIEYYWSQNEEPHQCLGTSPSRMTIQPNSLFLPSFQSFPRLLFIEQGEGIVGIQIPGCAETFDTGVQFQHQQQQQQQPRRMGEQGFDASADSHQKVHRFRQGDIIAIPAGAVHWAYNDANQEVVAVIVDDVNNPSNQLDLQAKISFLAGGISSEHIQGQQGQGRRQQQGQQGRRQQQQDRRRSQHQGRFQEDRLEKDNVFAGFDTELLAEAFNSDPQIVRALQESRNRGLIVRVQQPMQFVTPEEHQLEHMRQRRSGGPANGMEETICSAKLMFNLDNQREADVFNRQAGKLNMVNEHKLPILSLLDLSAEKGHLQPNALISPHWNLNSHTIVYVLSGDAQVQVVSNNGEAVLDEQVSRGDIFPVPQFFATTARAGQNGFEWVAFKTNRSPLKSPLAGYTSVFRAMPLDVITNAYEVSPSQAQNLKTNRETESLLFSPQRQQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BW85</accession>
    <name>A0AAD5BW85_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HTH TFE/IIEalpha-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_016671</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010975">
        <property type="protein sequence ID" value="KAI7729561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BW85"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005673">
        <property type="term" value="C:transcription factor TFIIE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039997">
        <property type="entry name" value="TFE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017919">
        <property type="entry name" value="TFIIE/TFIIEa_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002853">
        <property type="entry name" value="TFIIE_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024550">
        <property type="entry name" value="TFIIEa/SarR/Rpc3_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13097:SF7">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13097">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIE, ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02002">
        <property type="entry name" value="TFIIE_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00531">
        <property type="entry name" value="TFIIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51344">
        <property type="entry name" value="HTH_TFE_IIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH TFE/IIEalpha-type" evidence="4">
        <location>
            <begin position="6"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="182"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="188"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="209"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7729561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="241" mass="27860" checksum="2D396A4E3C6784A3" modified="2024-05-29" version="1">MSIEPFNKLVKLAARAFYDDITAKGDNQPKSGRSDNRGIAVVVLDALTRRQWVREEDLAKDLKLHLKQLRRTLRFFEEEKLVTRDHRKETAKGARAYSAAVAATADNLPGRDGEEKIKLHTHSYCCLDYAQIHDVVRYRLQRMKKKLKDELDNKNTVQEYDEYVKAYYAAILQRQKEQEEAAKREQDLLTNNIPTDVDPSSSRKRQRDDDGEDVEWEETPTAEVSGDEEGDDDDGIDWEEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BW90</accession>
    <name>A0AAD5BW90_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_030425</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010723">
        <property type="protein sequence ID" value="KAI7730595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BW90"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="113"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7730595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="165" mass="18534" checksum="CF00DF8444D95912" modified="2024-05-29" version="1">MKSSITGKRLSNPKLSLSARNQRVHGFFDVERLFLLSSRIPLRQHGHNSSNNLDGALTTIVKMSSRNVVVKEGFADQFQIPKPLSEAMCKDHTMHFQYTRTTGPARSLLAVKKATHTKKPPNTTPDNIRTPDSTYKKSDTTTSTSKRSFEEPGPISETKRIRLPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BX41</accession>
    <name>A0AAD5BX41_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RPW8 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_019975</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the disease resistance NB-LRR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010852">
        <property type="protein sequence ID" value="KAI7730024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BX41"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008808">
        <property type="entry name" value="Powdery_mildew-R_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766">
        <property type="entry name" value="PLANT BROAD-SPECTRUM MILDEW RESISTANCE PROTEIN RPW8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766:SF15">
        <property type="entry name" value="POWDERY MILDEW RESISTANCE PROTEIN, RPW8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05659">
        <property type="entry name" value="RPW8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51153">
        <property type="entry name" value="RPW8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0611">Plant defense</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="RPW8" evidence="5">
        <location>
            <begin position="1"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7730024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="651" mass="73699" checksum="4DC3598E53B8FD5B" modified="2024-05-29" version="1">MAEAVIIGAVVGDAVSKLSDAIIHVMKTTSQFRSKLTQLHQTITRINPIINEIQKLNQLLDRSKQETDMFIDQLKGAEKLVRKCEHIKWNFYKRYTHSLELDDLNASLLRFFQIDVQLQEARDVKEVLVVVKDVQRRMEDRRLGDQASSVPLLKASSVPLLKGNVIGFDDRVRELKVLMVKDSGVDDCEVVVVSAAGGCGKTTLVTMFCHDHDVQGIFGGNIYFATISNTPNLKVVIKNLLQKNQGGQQPDFTSDEDAIQQWGSFLGEQKSAVLLVLDDVWDVSIVKAFKFKLPGYKILVTSRSTFTQFRKYELHLLNHQDATDLFRYSAFSEGGSECSNIPDELVDELVKCCKRHPLALSVIGGLLKGTHITNWHIMLNNLSEGKKSVLDFEDMRLRLARSLDVFKEGSEIKECYLDLGLFPEDQKIAATALMDIWISLYNHDEKGSATLNNLFELSSKNLATLLPIRKDFVAIANYCDENAVMQHDMMRMLAINISSQEPAEHRKRLIVNAVGQNLPQLPQTINARLFSISTDEAFSFQWNDIRTPKVEVFVLNFMSKIYMFPQFMRNMESLTVLIITNYGYYFSDLHNFPAPKYLSRLTSIRLEHVSISSISASILGLVNLQRLSLIMCKFYAISFALRNLASLTAMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BY60</accession>
    <name>A0AAD5BY60_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein BUD31 homolog 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_009104</name>
        <name evidence="5" type="ORF">M8C21_011613</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the BUD31 (G10) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010703">
        <property type="protein sequence ID" value="KAI7730609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAMZMK010007957">
        <property type="protein sequence ID" value="KAI7742579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BY60"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001748">
        <property type="entry name" value="BUD31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018230">
        <property type="entry name" value="BUD31/G10-rel_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19411:SF0">
        <property type="entry name" value="PROTEIN BUD31 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19411">
        <property type="entry name" value="PROTEIN BUD31-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01125">
        <property type="entry name" value="BUD31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00322">
        <property type="entry name" value="G10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00997">
        <property type="entry name" value="G10_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00998">
        <property type="entry name" value="G10_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7730609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7742579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="145" mass="17252" checksum="12C5C14873944F60" modified="2024-05-29" version="1">MPKVKTNRVKYPEGWELIEPTLRELQGKMREAENDPHDGKRKCEALWPIFKIAHQKSRYIYDLYYRRKEISKELFEFCLDQGYADRNIIAKWKKPGYERLCCLRCIQPRDHNFQTTCVCRVPKHLREEKVIECVHCGCKGCASGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C105</accession>
    <name>A0AAD5C105_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Dicarboxylate transporter 1, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_033487</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC13A/DASS transporter (TC 2.A.47) family. DIT1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010092">
        <property type="protein sequence ID" value="KAI7733082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C105"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009706">
        <property type="term" value="C:chloroplast inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015140">
        <property type="term" value="F:malate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030676">
        <property type="entry name" value="CitT-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001898">
        <property type="entry name" value="SLC13A/DASS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42826">
        <property type="entry name" value="DICARBOXYLATE TRANSPORTER 2.1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00939">
        <property type="entry name" value="Na_sulph_symp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0934">Plastid</keyword>
    <keyword evidence="4" id="KW-1001">Plastid inner membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="89"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="119"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="153"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="188"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="218"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="260"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="312"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="360"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="400"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="431"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="459"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="487"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="528"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="19"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="19"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7733082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="554" mass="59053" checksum="AB5F54E77A95F875" modified="2024-05-29" version="1">MASLPLTTSVTLNFRSPTTVNHRLSTKQSPPLNLHRSNGLFHLRSSPSQSPSLKLKSLNKTLKFTVKATSATVPAKQDPSPAPVPWQGAAIKPLIASIATGIILWFVPVPNVGIITQPLPLGAVALLGLGACVLTKTLTFAAAFSAFGDPIPWLIALAFFFARGLGYSLVFSEALLAPAIPSVSARAGGIFLPLVKSLCVACGSNVGDGTENKLGAWLMLTCFQTSVISSSMFLTAMAANPLSANLTFNTIKQTIGWTDWAVAAIVPGLVSLIVVPLILYLIYPPTIKSSPDAPKLAKEKLEKMGPMTKNEIIMAGTLLLTIVILVEQCAADVSVFTAANSVKTLRLNIVDLERECVENYVGLWIFGGVLNVDAVTAAILGLSVLLITGVVTWKECLAEAVAWDTLTWFAALIAMAGYLNKYGLISWFSQTVVKFVGGLGLQWQASFGILVLLYFYSHYFFASGAAHIGAMFTAFLSVASALGTPPLFGAMVLAFLSNLMGGLTHYGIGSAPVFYGANYVPLGKWWGYGFLISVVNIIIWLGVGGVWWKFIGLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C2M1</accession>
    <name>A0AAD5C2M1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">spermidine synthase</fullName>
            <ecNumber evidence="3">2.5.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_003052</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>S-adenosyl 3-(methylsulfanyl)propylamine + putrescine = S-methyl-5'-thioadenosine + spermidine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12721"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:57443"/>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
            <dbReference type="ChEBI" id="CHEBI:326268"/>
            <dbReference type="EC" id="2.5.1.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amine and polyamine biosynthesis; spermidine biosynthesis; spermidine from putrescine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the spermidine/spermine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.16" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010010060">
        <property type="protein sequence ID" value="KAI7733238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C2M1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004766">
        <property type="term" value="F:spermidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008295">
        <property type="term" value="P:spermidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.140.10:FF:000003">
        <property type="entry name" value="Spermidine synthase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000048">
        <property type="entry name" value="Spermidine synthase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.140.10">
        <property type="entry name" value="Spermidine synthase, tetramerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00198">
        <property type="entry name" value="Spermidine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030374">
        <property type="entry name" value="PABS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030373">
        <property type="entry name" value="PABS_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001045">
        <property type="entry name" value="Spermi_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030668">
        <property type="entry name" value="Spermi_synthase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035246">
        <property type="entry name" value="Spermidine_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037163">
        <property type="entry name" value="Spermidine_synt_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002010">
        <property type="entry name" value="PRK00811.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00417">
        <property type="entry name" value="speE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11558:SF11">
        <property type="entry name" value="SPERMIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11558">
        <property type="entry name" value="SPERMIDINE/SPERMINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17284">
        <property type="entry name" value="Spermine_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01564">
        <property type="entry name" value="Spermine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000502">
        <property type="entry name" value="Spermidine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01330">
        <property type="entry name" value="PABS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51006">
        <property type="entry name" value="PABS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0620">Polyamine biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0745">Spermidine biosynthesis</keyword>
    <keyword evidence="4 8 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PABS" evidence="11">
        <location>
            <begin position="31"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7733238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="323" mass="35445" checksum="A92FA5815AF75240" modified="2024-05-29" version="1">MAEQTTTTTTVSNSPPLKRPREGDGISSVIPGWFSEISPMWPGEAHSLKVEKVLFQGKSDYQNVMVFQSATYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPNPKKVLVVGGGDGGVLREVARHASVEHIDICEIDKMVVDVSKEFFPNVAIGYEDPRVKLHIGDGVAFLKAAPEGSYDAVIVDSSDPIGPAQELFEKPFFESVAKALRPGGVVCTQAESIWLHMHIIEDIVANCRQVFKGSVNYAWTTVPTYPSGVIGFMLCSTEGPEVDFKNPINPIDENDNECKSVGPLKFYNREIHSAAFCLPSFAKKVIEAKARK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C3Z6</accession>
    <name>A0AAD5C3Z6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_017187</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009722">
        <property type="protein sequence ID" value="KAI7734459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C3Z6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7734459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="75" mass="7926" checksum="43DADD35FAB5B72D" modified="2024-05-29" version="1">MKVAEQLLCDGCLVKAVVDLRFGSLFPLGSSSSSFKAFEILILCNNVEVGSELSDSRNSTDVGAGKWISGEPSQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C6N6</accession>
    <name>A0AAD5C6N6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_023704</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009381">
        <property type="protein sequence ID" value="KAI7736087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C6N6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7736087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="271" mass="30535" checksum="6FA2F12338A1621C" modified="2024-05-29" version="1" fragment="single">AGDDGGVVVVRWRDEYRKGKGEREHRDNLTRSLMVILRLWYYAGGGGAAEVERLGGGAMAGDAFVICEYTVTSVFLLHFISDFFALHWDWIELFAGQDDRQRPVKKKNKKLQSSKSNKDNISFASIGRALLFCNKEEYKARDQIQIHRSGARETKGGCSLLLEVLSSNILRGVFKLVLFFPEGYPMASPNIWDVLGVYVFMNEGMEAKPMDSVLNCNVILIYVGTTSPELQPCAEFILAFFIILQAKSFFRSPAYNCKPVSQFTFHSRCGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C868</accession>
    <name>A0AAD5C868_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">NAD(P)H-quinone oxidoreductase subunit O, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_034046</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009148">
        <property type="protein sequence ID" value="KAI7737012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C868"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016655">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020905">
        <property type="entry name" value="NdhO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36728">
        <property type="entry name" value="NAD(P)H-QUINONE OXIDOREDUCTASE SUBUNIT O, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36728:SF2">
        <property type="entry name" value="NAD(P)H-QUINONE OXIDOREDUCTASE SUBUNIT O, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11910">
        <property type="entry name" value="NdhO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0618">Plastoquinone</keyword>
    <keyword evidence="1" id="KW-0874">Quinone</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-1278">Translocase</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="53"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="55"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7737012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="168" mass="18406" checksum="5D732A7FEE830039" modified="2024-05-29" version="1">MAMTYSSSFTSFPYTKPYTPTITITKPGLLPATGSHGGGRGRFCFSVKAEKSAESADSTPAAAATATETPPAAAKPPPKKPVYSMKKGQIVRVDKEKYLNSINYLSVGHPPYYKGLDYIYEDRGEVLDIRIFETGEYALIAWVGVPTAPAWLPTDMLIKSDKLDYERI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C8R4</accession>
    <name>A0AAD5C8R4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_011123</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009349">
        <property type="protein sequence ID" value="KAI7736159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C8R4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11119">
        <property type="entry name" value="XANTHINE-URACIL / VITAMIN C PERMEASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7736159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="60" mass="6649" checksum="4DFDD1939FB18524" modified="2024-05-29" version="1" fragment="single">IFLATMRAIQDALIVALSEQIILGYSQLWAICSRFFSPVGMVPVIELDGFGLFDRGFLVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C9I6</accession>
    <name>A0AAD5C9I6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_001548</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NEMF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009080">
        <property type="protein sequence ID" value="KAI7737225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C9I6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990112">
        <property type="term" value="C:RQC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072344">
        <property type="term" value="P:rescue of stalled ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990116">
        <property type="term" value="P:ribosome-associated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.310.10">
        <property type="entry name" value="ibrinogen binding protein from staphylococcus aureus domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021846">
        <property type="entry name" value="NFACT-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008532">
        <property type="entry name" value="NFACT_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051608">
        <property type="entry name" value="RQC_Subunit_NEMF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15239">
        <property type="entry name" value="NUCLEAR EXPORT MEDIATOR FACTOR NEMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15239:SF6">
        <property type="entry name" value="RIBOSOME QUALITY CONTROL COMPLEX SUBUNIT NEMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11923">
        <property type="entry name" value="NFACT-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05670">
        <property type="entry name" value="NFACT-R_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05833">
        <property type="entry name" value="NFACT_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="8">
        <location>
            <begin position="915"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="137"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="453"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="648"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="715"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="775"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="329"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="453"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="715"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="787"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="850"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="861"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="889"/>
            <end position="899"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7737225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="1074" mass="119988" checksum="38D2D2967F155190" modified="2024-05-29" version="1">MESGVRLHTTAYLRDKSNTPSGFTLKLRKHIRTRRLEDVRQLGYDRIVLFQFGVGANAYYIILELYAQGNIILTDSDFMVLTLLRSHRDDDKGFAIMARHPYPIENCRLFERTDSAKLQSTLTSFNNKKIEQHVEDGLGVNDTSGAPQAKKGNHKAVKSGDSAKKKDSTNTKQATLKVVLGEALGYGPALSEHIILDAGLTPNLKVSKDMKLEDSSIQDLTEGVKRFEDWLEDVISGAKIPEGYILMQEKKIGKDHIPVESSGTDQLSSEIDAYDFLIYDEFCPLLLNQFRLRVSVKFETFDAALDEFYSKIESQRAEQQQQAKESSAMQKLSKIRNDQENRVQLLKKEVDRAVTMAELIEYNLEDVDAAILAVRVALANGMNWDDLTRMVKEEKKSGNPVAGLIDKLHLERNSMSLLLSNNLDEMDDDEITQPVEKVEVDLSLSAHANARRWYEQKKKQESKQEKTLTAHAKAFKAAEKKTRQQLSQEKSVAAISHIRKVHWFEKFNWFISSENYLVISGRDAQQNEMIVKRYMSKGDLYVHAELHGASSTVIKNHKPDNPVPPLTLNQAGSFTVCHSQAWNSKIVTSAWWVYPHQVSKTAPTGEYLTVGSFMIRGKKNFLPPHPLVMGFGILFRLDETSLGSHLNERRVRGEDEGLNESEDTEPFKELSDTGSENEAPDAEYPVHMSDLSTDNQKLANSSSEDNHKMANLSSEDNQKLANLSSEADSNHEISSISGQDAVEEKTSAATVSPELEDLLDRALELGSAAKKYGLLASSQMDSDIDNNTEKKKETVSREKPYVSKAERRKLKKGQTHSEGGDGGHVEKKKHKAEQKNVENLKVDGGGKVSRGQKGKLKKMKEKYGDQDEEERKIRMALLASAGKAKSNEPEVQSETITTSKELKPVGSGNEDAIKRCYKCKKVGHLAKDCQEDANGVVQLNEAERMAMEEDDIKEIGEDEKEKLNDVDYLTGVPLPNDILLYAVPVCAPYAAVQSYKYRVKIIPGTAKKGKAAKMAMNLFGHMPEASQREKELMKACTDPELMAAIIGSVKISAAGLTQLKQKQKKGKKAASKQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C9M1</accession>
    <name>A0AAD5C9M1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_032147</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008927">
        <property type="protein sequence ID" value="KAI7737883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C9M1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009534">
        <property type="term" value="C:chloroplast thylakoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025067">
        <property type="entry name" value="DUF4079"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34679">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34679:SF2">
        <property type="entry name" value="OS02G0122500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13301">
        <property type="entry name" value="DUF4079"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="170"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="204"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="233"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7737883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="267" mass="28877" checksum="5183ED2A4E24E0AB" modified="2024-05-29" version="1">MAATLAFLQIPTTTIISSKSSSHSQQLPPIPSSSKVSFCPMSSNSISLPLSAAAAVTLFLNPQDALAAGGEFGILEGRSLALIHPIVMGGLFVYTLYAGYLGWQWRRVRTLQDQINELKKQDLPLTVTVTPEGTPAAAAAADPSPVQLQIQRLSEERKELIKGQYREKHFNAGSILLGFGVFESIGGGLNTYLRTGKLFPGPHLYAGAAITVLWAAAAALVPPMQKGNETARTLHIALNILNVLLFIWQIPTGWDIVLKVFEFTKWP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C9V4</accession>
    <name>A0AAD5C9V4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Peptidyl-prolyl cis-trans isomerase</fullName>
            <shortName evidence="6">PPIase</shortName>
            <ecNumber evidence="6">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_032161</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the cyclophilin-type PPIase family. PPIL3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="6"/>
    <dbReference type="EMBL" id="JAMZMK010008927">
        <property type="protein sequence ID" value="KAI7737897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C9V4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01928">
        <property type="entry name" value="Cyclophilin_PPIL3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000012">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024936">
        <property type="entry name" value="Cyclophilin-type_PPIase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044666">
        <property type="entry name" value="Cyclophilin_A-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625:SF2">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE-LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001467">
        <property type="entry name" value="Peptidylpro_ismrse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4 6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="7">
        <location>
            <begin position="6"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7737897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="154" mass="16678" checksum="0E797ECB9359C618" modified="2024-05-29" version="1">MSVTLHTNLGDIKCEIACDEVPKASENFLALCASGYYDGTIFHRNIKGFMIQGGDPTGTGKGGTSIWGKKFNDEIRESLKHNARGVLSMANSGPNTNGSQFFITYAKQPHLNGLYTIFGKVIHGFEVLDLMEKVILCMRIYPNGGSWGPATGGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CAN8</accession>
    <name>A0AAD5CAN8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">M8C21_009018</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">AA19_3_7</strain>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the RING-type zinc finger family. ATL subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="JAMZMK010009096">
        <property type="protein sequence ID" value="KAI7737179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CAN8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16461">
        <property type="entry name" value="RING-H2_EL5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000187">
        <property type="entry name" value="E3 ubiquitin-protein ligase ATL6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053238">
        <property type="entry name" value="RING-H2_zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14155:SF263">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE ATL6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14155">
        <property type="entry name" value="RING FINGER DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="17" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042127575" description="RING-type E3 ubiquitin transferase" evidence="17">
        <location>
            <begin position="27"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="42"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="18">
        <location>
            <begin position="123"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="284"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="335"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="338"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="362"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAI7737179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="372" mass="40738" checksum="F6B5CDC32F19D4BE" modified="2024-05-29" version="1">MNYIKNITTFYMLLLLLISSIYSIQAQPSPPPDDYQYARFSPSMAIIIVVLVAALFCMGFFSIYIRRCSEDGNPSARGGLSMRRAARLAAASRGLDADVLETFPTFAYSTVKGLKIGKGALECAVCLNEFEDEETIRLIPKCDHVFHAECVDAWLEHHVTCPVCRCNLVPKPGESTHGTNESEFVMIENNQQTPVSEENTDISIQVGDTVSNPNPVLNRVKSANPYRPPRSKSVRGPALFEKFRSHSTGHSLVQVGEDTERFTLRLPEDVRKQVIDNALLNRSGSTVRLPGEGSAKKGYRTGSSGSGSYKRMGSLDRTVFSRAPSFLSRAFSVRSQKSASANTGASTSTTSDKVPLYNIGPKSDEVVGRPPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CCG6</accession>
    <name>A0AAD5CCG6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_013767</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008675">
        <property type="protein sequence ID" value="KAI7739102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CCG6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019199">
        <property type="term" value="F:transmembrane receptor protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044812">
        <property type="entry name" value="CERK1/LYK3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46204">
        <property type="entry name" value="CHITIN ELICITOR RECEPTOR KINASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46204:SF27">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="24"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="1"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7739102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="270" mass="29675" checksum="8869EB8D12463A8D" modified="2024-05-29" version="1">MLLADENGNYPPLRISSETSLSGYAIGGMTVGVVAVVLFLSGCLYIRVYKKKNAETSSIVLQNAQVQVQVQVQYTPVQSITIKKSVEFSYEELSTATCNFSLANKIGQGGFGAVYYAELRGKKAAIKKMDRQASREFLAELKAGHVGWEMGQIIHGYVQYGDVSPKVDVFAFGVVLYELISAKEAIIRSNEPDAEPKGLVALFDEVLSQADPKEDLVKMIDPRLGDKYTLYSVYKMAQLAKVCTCEDPQRRPSMRSIGIALMSLLSSTED</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CE95</accession>
    <name>A0AAD5CE95_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Sec-independent protein translocase protein TATC, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_002054</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008700">
        <property type="protein sequence ID" value="KAI7738891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CE95"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033281">
        <property type="term" value="C:TAT protein transport complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009977">
        <property type="term" value="F:proton motive force dependent protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043953">
        <property type="term" value="P:protein transport by the Tat complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00902">
        <property type="entry name" value="TatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019820">
        <property type="entry name" value="Sec-indep_translocase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002033">
        <property type="entry name" value="TatC"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00945">
        <property type="entry name" value="tatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30371:SF6">
        <property type="entry name" value="SEC-INDEPENDENT PERIPLASMIC PROTEIN TRANSLOCASE TATC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30371">
        <property type="entry name" value="SEC-INDEPENDENT PROTEIN TRANSLOCASE PROTEIN TATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00902">
        <property type="entry name" value="TatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01840">
        <property type="entry name" value="TATCFAMILY"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01218">
        <property type="entry name" value="TATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="129"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="180"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="212"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="263"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="296"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="320"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7738891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="345" mass="37935" checksum="105987B3D2E28EB8" modified="2024-05-29" version="1">MMGTNAFTNLQLSTNSCTKCVLSRTNNPNLLQMNQKKTNLCISNKRRNNRIMCFKARFPPDEVKDMQRKLGIGGGGPVVEQQTKVVNEVKQDSQVKGALYNFLNPDKELLPEDKEMSLFDHLEELRERLFVSVLAVGGAILGCFVYSKELIVLLEAPVSSQGVRFLQLAPGEFFFTTLKVSGYCGILLGSPVILYEIIAFVLPGLTKSERKFLAPIVLGSSVLFYTGIAFSYSVLTPAALNFFVTYAEGVVESLWSIDQYFEFVLVLMFSTGLSFQVPVIQLLLGQVGLVSGDQMLSIWRYVVVGAVIAAAIVTPSTDPLTQLLLAGPLLGLYFGGAWTVKLMGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CFZ4</accession>
    <name>A0AAD5CFZ4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">4-coumarate--CoA ligase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_027219</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Phytoalexin biosynthesis; 3,4',5-trihydroxystilbene biosynthesis; 3,4',5-trihydroxystilbene from trans-4-coumarate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008325">
        <property type="protein sequence ID" value="KAI7740902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CFZ4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106286">
        <property type="term" value="F:(E)-caffeate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009698">
        <property type="term" value="P:phenylpropanoid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12118">
        <property type="entry name" value="ttLC_FACS_AEE21_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.30:FF:000008">
        <property type="entry name" value="2,3-dihydroxybenzoate-AMP ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000003">
        <property type="entry name" value="Long-chain-fatty-acid--CoA ligase FadD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006020">
        <property type="entry name" value="PRK08162.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43859">
        <property type="entry name" value="ACYL-ACTIVATING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43859:SF4">
        <property type="entry name" value="BUTANOATE--COA LIGASE AAE1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-0587">Phenylpropanoid metabolism</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="7">
        <location>
            <begin position="21"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="8">
        <location>
            <begin position="455"/>
            <end position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7740902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="553" mass="60820" checksum="00FA7CE77942AA6B" modified="2024-05-29" version="1">MEGTIRCSANYIPLSPISFLERSSVVYRDRLSVVYGDHVKFTWKQTHERCVKLASSIASRFRISRGDVVAVLAPNIPAMYELHFAVPMAGAVLCTLNIRHDSKMVSTLLKHSNAKLIFVDHQFIDVAKGAIEIMSKSSATVPQIVLILVSGELPVLTKSEVVEYEALLAMGSLDFEIRRPIDECDPIALNYTSGTTSNPKGVVYSHRGAYLNSLAAVLLNEMRSMPVYLWTVPMFHCNGWCLTWAVAAQGGTNVCLRAVTAKGIFGSISQHGVTHMGGAPTVLNMIINAPETERVPLPGKVTVMTGGAPPPSQVVFGMEQLGFDVCHAYGLTETYGPGTVCTPKPEWDALPLESQAKLKSRQGVNHLGLEEVDVKDPVTMKSVAWDAKTMGEVMFRGNTVMNGYLNDINATQEAFKDGWFRSGDLGVRHSDGYIELKDRSKDIIISGGENISTIEVESVLFRHPAVLEAAVVGRPDEHWGETPCAFVELKQGYKLKEDELIAYCRNNLPRYMAPRTVVFRELPKTSTGKTQKFVLREWAKAMGSLPSRGKSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CIB0</accession>
    <name>A0AAD5CIB0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_000825</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008207">
        <property type="protein sequence ID" value="KAI7741365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CIB0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7741365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="67" mass="7728" checksum="03A2049D6D44CEA4" modified="2024-05-29" version="1">MIWWLICSERKLKVLFVVDNCHPPIVLEDIVLLFAEQEKVQAGFDELSVMFLSLAGNMLSILNYICQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CIF5</accession>
    <name>A0AAD5CIF5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_029943</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008059">
        <property type="protein sequence ID" value="KAI7742184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CIF5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11017:SF573">
        <property type="entry name" value="ADP-RIBOSYL CYCLASE_CYCLIC ADP-RIBOSE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11017">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="738"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7742184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="738" mass="84941" checksum="B2A6B9D8B16EB9CD" modified="2024-05-29" version="1" fragment="single">ITFGCMICCNKCVGKFFTGNHRHDGNHIAIKYHEDVVDILLSNPKGTEAVEVINQEPYKGEVNDCFITDLTCFSNMTKLKFLRISNVHFPQGLKYLSNDLRILEWYGCSLKFLPSMFKPKHIYELEVCSSQLERLWKKDLELPNLRSINLSFSKNLTKVPDITSAWNLVKLNLQGCTNLTRLHESVLLHKRLRYLNLKGCTCLQSLGRRSMEMEALEALLLSGCSKLENIPKFGKNMKRLEHLYVDGTRIKSLPENLGQMHDLRNLDASGTFIEELPSSIYRLKRLRLLNYLRDEVHERKWTVKLREKYWMTPEAVFEIVGAGSHIPFEFVHPVSGGLILEGPWIGVVICAVISVHHVDVYMESKYTVTAHIHLGEKHWKIPVPINFLVAESETHVDPEGQSHENDSNLHVTKFSARFIDNEYMMHLKRPEVLSGCTREWKYAQCVSSYYAMSLGASVLYNDFVQSLKDVNDGWFSVKLALEQIMIKSRAENYSYIEILEQLNDIEAPQCVGFSDEFITVLECVFKHTCSIVPFFNAELQRVTTLEPEKLAKKKFKRVKALARISSITILAVFCMMAAYIGDLEPLKELKQSALDSLWQRLFLPFADKRKFFTEDGLFIDTHCSSELQISEFYENAIFLQNLMNKMGRSSCFTKDNEEEMTSVIDKLQMNIHKLSYTIQAMSSHADQYSDYIGNARKHVKAFRRHPKDDTSIIFTIPVLFKSNLGTAKTRAIVITREL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CL64</accession>
    <name>A0AAD5CL64_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_029576</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007876">
        <property type="protein sequence ID" value="KAI7742880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CL64"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7742880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="104" mass="11266" checksum="7ACC3F00149A0DEE" modified="2024-05-29" version="1">MCYSSPWKGKCSAGTTATAVVAAPKEEDTTSTVVRMFGNTTSTLDLKSAATDNVETNNCYFCNISPAVDAAPVFNVYFNLLVRSATWRSGLNNGCQCLRYLFNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CLN6</accession>
    <name>A0AAD5CLN6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_005336</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007894">
        <property type="protein sequence ID" value="KAI7742791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CLN6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7742791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="80" mass="8769" checksum="4FCA412CDDBD9098" modified="2024-05-29" version="1" fragment="single">YKTIDLTDAAEKDPRTGITTCIVNNALFLQQPDTLNTTSSSASVSMTPTPFTPDIATSNKKHKEAIAKAVCLNTRRRKRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CMD2</accession>
    <name>A0AAD5CMD2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">F-box protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_017302</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Acts as a component of a SCF E3 ubiquitin ligase complexes.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Component of the SCF-type E3 ligase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007538">
        <property type="protein sequence ID" value="KAI7744422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CMD2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd22151">
        <property type="entry name" value="F-box_AtGID2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.50:FF:000045">
        <property type="entry name" value="F-box protein 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045464">
        <property type="entry name" value="Hrt3/FBXO9_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12874:SF9">
        <property type="entry name" value="F-BOX ONLY PROTEIN 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12874">
        <property type="entry name" value="F-BOX ONLY PROTEIN 48-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19270">
        <property type="entry name" value="FBO_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="F-box" evidence="3">
        <location>
            <begin position="57"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU369085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7744422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="327" mass="37527" checksum="7B8540B1A47AA11A" modified="2024-05-29" version="1">MASDYLTNIVAELESASQLRAAGFFITRRPWLDLYGVNVRPVAPFGSTYGKPVVDSSLIHRSLPDELLFEIFARMTPYNLGKAACVCRKWRNTVRNPVFWRNACLKAWQISGVVENYKLMQSHYDSSWRKMWLLRPRVRMDGIYVSRNTYIRAGVAEWKVTNPVHVVCYFRYLRFYPSGKFLYKNSSEKLKDVMKFMNFRSTKAEICHSGRFTMSEDKVEGALLYPGMRPTIWRIRLRLRGTTAGANNRMDLLSIVTSGVQESEVPGPDGDILGVVEGWQDDETHNPEVPAISHTRGLTPFVFVPFEEAETSVLNLPVERMDYYVPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CQB5</accession>
    <name>A0AAD5CQB5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_000401</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007014">
        <property type="protein sequence ID" value="KAI7746236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CQB5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7746236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="55" mass="6117" checksum="DA4E02607394077F" modified="2024-05-29" version="1">MRNGKNADTWRSQQRHEIKGGFGLQVLKVVQVASQSLARTGSCLECWQQVLKSSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CQQ5</accession>
    <name>A0AAD5CQQ5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">C2 NT-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_000402</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007014">
        <property type="protein sequence ID" value="KAI7746237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CQQ5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019448">
        <property type="entry name" value="NT-C2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31182">
        <property type="entry name" value="C2 NT-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31182:SF15">
        <property type="entry name" value="F26K24.5 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10358">
        <property type="entry name" value="NT-C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51840">
        <property type="entry name" value="C2_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2 NT-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="625"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7746237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="625" mass="70154" checksum="FE17CD0E5370765C" modified="2024-05-29" version="1" fragment="single">AMATTAVKEIQGEIGGEEDGRAVEIRWKGPKVSLSSFRRTVKTNCTREEKVTHSNGVVHWDEEFHTISTLSCSKDNHFNPWDIGFTVLDLNPTAVLGDGLSPGPKSKISIIGTASLNLAEYVSAAEEKDLDLTIPLTLSCESTGPRPSLHVSDAYNSLMMSVVVKEVNSPLKIFHKVKEDDDVTFRKSFSYGTLAYANCTTGSVHEDGVYYSNRKSDVGCSQNDDSDASVSEPYVIPNQKRVIFPWKKRKMNLRSPKTKGEPLLKKAYAEEGGDDIDFDRRQLSSAQSLGWYMLNDESSVSEFGDDDFAVGNWEHREIISRDSNMKLRTQVFFASIDQRSERAAGESACTVLVAVIADWFQNNHNHMPIKSQLDSLIREGSLEWRNLCENESYKAQFPDKHFDLDTILEANLRPLLVVPEKSFIGFFHPEEIQEGSFDFLHDAMSFDTMWDEISRESEPRVYIVSWNDHFFVLKVDSDAYYIVDTLGERLFEGCNQAYILKFDRNTIIYQLPTSESGSDDSNRKSTSEAMEPVKNLGENEVICEGKESCKVYLKCFLAAIPIRELQADMKKGLASSIPLHHRLQIEFHYTQLQPPPATATANPDLVPVTATSAPEVDDTAQLVAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CQX3</accession>
    <name>A0AAD5CQX3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_010356</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007023">
        <property type="protein sequence ID" value="KAI7746159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CQX3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47641:SF1">
        <property type="entry name" value="GOLGI-ASSOCIATED OLFACTORY SIGNALING REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47641">
        <property type="entry name" value="PERIAXIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="104"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="125"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7746159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="161" mass="17540" checksum="5BCD2E98E4BE8F3F" modified="2024-05-29" version="1" fragment="single">TLASIVSESRNLLEVPEFPKPEVPEIPKPALPDIPKPKLPEVPKPKPAIPEIPKPELPKPEVPKAPEFHDLPKPTLPEVPKPEVPVLPKPEIPEIPKPTLPDIPKPKLSEVPKPKVPVLTKPEIPELPKPALPEIPKPTIAEIPTIPGLPKDLPNPSIPHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CSD9</accession>
    <name>A0AAD5CSD9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_023188</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007179">
        <property type="protein sequence ID" value="KAI7745656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CSD9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7745656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="223" mass="24974" checksum="11A1633B212FADA7" modified="2024-05-29" version="1" fragment="single">MENYQFRFPITTTTSYTNDPFKNSERRGSGVVEEPFKNSEKQDPNNMNPDPVAAQLAAIASPMESLQHVLQSSCIRLNERNPLVVPTNLKTWVLPERRGSLKMDFEGRKVLSCGEKYDPAHRCKTGTFKLLKTSDNQEVPFGTKEEASGVQEELAEISLHVTFSKSHMSTMKLQGMLGTTEVLILVDSRSTLNFISDSLVRQLKIATKFMTQFGVQIGNGDII</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CSW8</accession>
    <name>A0AAD5CSW8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_012658</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006860">
        <property type="protein sequence ID" value="KAI7747007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CSW8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043539">
        <property type="term" value="F:protein serine/threonine kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047173">
        <property type="entry name" value="STRAD_A/B-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48014">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE FRAY2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48014:SF7">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE BLUS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="10"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="444"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7747007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="486" mass="53900" checksum="104AF3E18E171F97" modified="2024-05-29" version="1" fragment="single">SNYPLDPNSYQILHEIGRGCSAIVYKAACLCTTTTTSTSTVVAIKSIDLDCSTTGDFDSIRREAKTMSLLSHPNVLKAHCSFTVGRRSLQSIMTSSFPNGISETCISIILKETLMGLCYLHDQGHLHRDIKAGNILMDSNGSIKLADFGVSASVYESSSRCLNLNEITGTPYWMAPEVIHSHNGYSYKADIWSFGITALELAHGKPPLAQLPFSESLVMKITRGMRFSEYQKEEEKGKKPKLSKYFKEMVGLCLEQDPCKRPSAAKLLKHYFFKNCKGCDFLVKNLLHGLPSVEFRFKERSASMSEEHDDDDEEEEEGLVGSNVKKHRRISGWNFSLEACQHGPVFPVVDSISDLSTNTSCLEEDSAKQVAFSGESVVSENEGSSRSNVTEDGSCVDKEVVMGSLMTLKRSLDDQREKVVYMLAVVGAEETGGSGGGDEYGGKEEKLMQVIEKLKLELENEKRKRTSLEMELDFLKLHITTNTSHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CT79</accession>
    <name>A0AAD5CT79_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_024656</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007046">
        <property type="protein sequence ID" value="KAI7746091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CT79"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009506">
        <property type="term" value="C:plasmodesma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004714">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045272">
        <property type="entry name" value="ANXUR1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27003">
        <property type="entry name" value="OS07G0166700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27003:SF383">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE, NON-RECEPTOR JAK_TYK2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="25"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="353"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7746091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="439" mass="49874" checksum="C8BE2385BDB1F3ED" modified="2024-05-29" version="1" fragment="single">MSSFDHKYAHLRLPLKDIKIATDNFADENIVRRDGSGNEYKGQLVLSSGQLIDILARRLDPRWGQGIKEFQTEIMMLSNLNHQNIVSIVGFCDEDEEKIIINKFEAKGNLYEYLSDPVTLPWTQRLQICLGIARALSYIHYDNQRSFSVIHRDVCSFKILLGDNWEAKLSGFELSIMQPAAKRQGLILDYPCGTHGYCDPAYIASETVNHKSDVYSFGVVLFEVMTGRKAHNPDNRDLKFSAKTAKSHFENGNLNDIIHPDLQKQMDQGSLKKFSEAAYYCVNDQRSQRPNLDQLIFALDQALKLQLAHEESLSLKEPSTLADEGTLSNYWKGKKLEHLRIGFDAIKSATNNFDETCLIGKGGYGWVYKAELEHFDPYATKGDRESEVPRRHSIVAIKYIIPREDAQGEQGFTAEIETLSCCKHPNIVSLLGFCHEHPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CTM4</accession>
    <name>A0AAD5CTM4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_002778</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006709">
        <property type="protein sequence ID" value="KAI7747674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CTM4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7747674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="150" mass="16580" checksum="67DE165C7BF0D987" modified="2024-05-29" version="1" fragment="single">TYAVFFTTTTLPPVLGQPARQVRWNAFSGVASNGPIQLRLRSVDGGQGGGGTPSLGGGWWRRLSVAAFALLWRLYLRVDVTVSLSRLGAVVLGFRLEQEHVIASTTPVVAWMLLCHRNNVSHRIVSPRLYCYIQAADIFSNRFVLVEKLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CTT8</accession>
    <name>A0AAD5CTT8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF538 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_014371</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006662">
        <property type="protein sequence ID" value="KAI7747842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CTT8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.240.10">
        <property type="entry name" value="At5g01610-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036758">
        <property type="entry name" value="At5g01610-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007493">
        <property type="entry name" value="DUF538"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31676:SF130">
        <property type="entry name" value="DUF538 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31676">
        <property type="entry name" value="T31J12.3 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04398">
        <property type="entry name" value="DUF538"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141562">
        <property type="entry name" value="At5g01610-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7747842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="178" mass="20391" checksum="633B815244131C8F" modified="2024-05-29" version="1">MEKALIKVGSIKAGSFWVSKKAKEEISNISQDLSTFSNTVEKKAKWVFDKLKGKPSKPLPDFLREYNLPPGLFPQNITCYEFDESKLKLVVHLPYPCEVCFKDQSVVRYATRVKGIVSRGKFTVIDGMKTKVLVWVKVTSVVVENYRSDKVWFTAGVKKSRPRDAYETPRDATRVEDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CUT8</accession>
    <name>A0AAD5CUT8_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_028876</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006615">
        <property type="protein sequence ID" value="KAI7748012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CUT8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005655">
        <property type="term" value="C:nucleolar ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000172">
        <property type="term" value="C:ribonuclease MRP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039182">
        <property type="entry name" value="Pop1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055079">
        <property type="entry name" value="POP1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009723">
        <property type="entry name" value="Pop1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012590">
        <property type="entry name" value="POPLD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22731">
        <property type="entry name" value="RIBONUCLEASES P/MRP PROTEIN SUBUNIT POP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22731:SF3">
        <property type="entry name" value="RIBONUCLEASES P_MRP PROTEIN SUBUNIT POP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22770">
        <property type="entry name" value="POP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06978">
        <property type="entry name" value="POP1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08170">
        <property type="entry name" value="POPLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Pop1 N-terminal" evidence="4">
        <location>
            <begin position="55"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="POPLD" evidence="5">
        <location>
            <begin position="442"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="domain" description="POP1 C-terminal" evidence="6">
        <location>
            <begin position="682"/>
            <end position="769"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF06978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF22770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7748012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="780" mass="87421" checksum="F29F8B6348040F4A" modified="2024-05-29" version="1">MTTDDGSKQLKCHAIPPGELLVQKFTESRAPELDSLYSTIATRLNNDFRSQRDKRRRTTSFDNRASKTRFRTKQKVGILNQPKRDENLEKIVSKAPPRHIRRKKELNNNLESGFGSSGDGTKRLRTHVWHAKRFTMIKRWGFHLPLGLHGRGKGSRALLKWLKNGTVIHDASYYSAVQLQGTQDSLFAILSTVMSPFSSSDSENVLSGGIYASSMLHHAEAINSRTIAPVIYMWRPIQQSTDGELECDRTKGFRQLWIWIHAAALTEGYNALKSACESQGKANEDFASVRCISLEGQFGTIEVIGSKASQLLQNILHPVSRRVIHLSDKSSVLRNSSSMGAVGEHIPCSGIFPLTVDDPRALTKENTRFDANYTDLWDAEKGLYCPVEESVLCMEKHHQRLLSFCLTEKTPADINASTRIHSSRICPIMLLRRNNLMDSVTRWTIILPLSWVKAFWVPLVSIGARAIGLREMSWVSCEAGIPNFPSEFPECDSYSSLMEAEVAAMDQEAALRPASVKTFSIPVPPPWNCVRLAFGMNPNESKTTQICSNESDAVAEPALSDNDIVVARSSRMLSAFLSAINDGRLPLFPYFSDKNTCLSKYMKDEKILDHDPVTDSFLGNRCRKVCFVRVILQAYKEGAIEDGAVVCAPRLADIKLWTSGSNNKGELQVPQSSIATYFVKQDSGKWEFQVPEDTAAMESNRWPIGFVTSGFVQGSKKPLAGGLCDAVLLARLRHEQWSSVPLKQRKKEIYVLTRNLRSTAYRLALARIVLEHQQEDLDYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CUW4</accession>
    <name>A0AAD5CUW4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_014378</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006549">
        <property type="protein sequence ID" value="KAI7748349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CUW4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7748349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="64" mass="7253" checksum="E47DF6A5A0417381" modified="2024-05-29" version="1">MLVSAKKKNPLAWDCALRISHGLAYHRLLPFPLLPSPSFSAVPSYVLIRFRSATGTQVRFFFCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CUW5</accession>
    <name>A0AAD5CUW5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_007600</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006526">
        <property type="protein sequence ID" value="KAI7748442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CUW5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051973">
        <property type="entry name" value="tRNA_Anticodon_Mtase-Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14344">
        <property type="entry name" value="WD REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14344:SF3">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="3" id="KW-0853">WD repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7748442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="120" mass="13206" checksum="F845EC05ABD8B5AA" modified="2024-05-29" version="1">MCSLCFIVDRTNSFQLAFITTGCEDGTVRLTRYTSGVDNRSASKLLGEHVGGSTVRSLCSVKKVHTFTVYMTSKAGAIIKHGTDLDDQKDHFLLISVGAKRVITAWKHKTTSTTKSEMLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CVW4</accession>
    <name>A0AAD5CVW4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cupin type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_031271</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the germin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006640">
        <property type="protein sequence ID" value="KAI7747904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CVW4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02241">
        <property type="entry name" value="cupin_OxOx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000005">
        <property type="entry name" value="Germin-like protein subfamily 1 member 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006045">
        <property type="entry name" value="Cupin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001929">
        <property type="entry name" value="Germin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019780">
        <property type="entry name" value="Germin_Mn-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31238">
        <property type="entry name" value="GERMIN-LIKE PROTEIN SUBFAMILY 3 MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00190">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00325">
        <property type="entry name" value="GERMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00835">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00725">
        <property type="entry name" value="GERMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0052">Apoplast</keyword>
    <keyword evidence="6 10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 8" id="KW-0464">Manganese</keyword>
    <keyword evidence="5 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042087744" description="Cupin type-1 domain-containing protein" evidence="11">
        <location>
            <begin position="22"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="Cupin type-1" evidence="12">
        <location>
            <begin position="60"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="31"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7747904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="306" mass="33220" checksum="9A10B97BFD529176" modified="2024-05-29" version="1">MSNKLIILSFVALTFACLAFAYEPKPLQDFCVADPNTSVKVNGLVCKDPKQVQAEDFFFSGLHLRGNTSNAVGSRVTTVFAAQLPGLNTLGISLVRIDYAPWGLNPPHTHPRATEILTVIEGTLKVGFVTSNPDNRFITKVLQKGDVFVFPVGLVHFQQNVGNGYAVAIAALSSQNPGAITIANAVFGANPPISGDILAKAFQVDKSAPVTDLEFRGVSRRKVQRSIGENRGYRSILYSFDVKFLDNQVIIGQIWSRKGYGYPYHRGLHVGFDGIELVLHITGTEAIALARLLGVVRAQGRPLLES</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CXX6</accession>
    <name>A0AAD5CXX6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_024403</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006356">
        <property type="protein sequence ID" value="KAI7749467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CXX6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7749467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="161" mass="17270" checksum="DAD5A46A7B23B42A" modified="2024-05-29" version="1" fragment="single">LGPTSRLPQRPAYITGIIKGHGRLCWKVSCEDPCWDDGFSKASEAGGEGGSSGSVVIRRCGFILQNGGGGSVVEWCTLCTGVCPCSNVVTACRLVGFHEDVGGLTRSKHEDFGGERFYIRSISSHHCKLVAALITLIRLVSPESTEANLCLSIYKDLQVDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CY30</accession>
    <name>A0AAD5CY30_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">WD repeat-containing protein 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_007603</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the WD repeat WDR6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006526">
        <property type="protein sequence ID" value="KAI7748445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CY30"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051973">
        <property type="entry name" value="tRNA_Anticodon_Mtase-Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14344">
        <property type="entry name" value="WD REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14344:SF3">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="3 7" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="226"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="996"/>
            <end position="1010"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="871"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1031"/>
            <end position="1076"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7748445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1224" mass="136177" checksum="FEC6484FB440736A" modified="2024-05-29" version="1">MESKQYLGEISALCFLHLPPPSPSLPFLLAGTGSQLLFYNLATSTMLASFQVFDGLRVHGISCLPSPDTETTFTVAVFGERRLKLFLFDIRLSDYDNQTLVNLVSVQSLPNLGHWILDVCFIQDSVNEGRQFIAIGCTDNSVYFWDMLTSTVSYQVSSPDICLLYTMRMWGDTIDSIHVASGTIFNEIIVWKVVCGDYTPCLDSPLKDSEAHVCNRQYKAAPVCRLAGHEGSIFRITWSLDGSKLVSVSDDRSARIWEVFNAKKDCNNDAEVTISPSSGPVLFGHSARVWDCCMSDSLIVTVGEDCTCRVWGLDGTQLRIIKEHIGRGVWRCLYDPVSSLLVTAGFDSAIKVHQLHSSLPMGFTKCNGVEEDEKKQIFTIQIPNPNRTGLMDSKSEYVRCMHFASESALYVATNNGVLYLANISDTGDVAWTQLFRASENIAIVCISVFPGATSCVDNWIALGDGKGRLTVVRVVGLPTPEVNFSLTWSAEAERQLLETFWCESLGPTFIFTADPRGKLKLWRIESFSQYLVAEFTSCFPIRIICLDASFKEEVLVCGDLRGNLVLFPLLRDLLLSTPVSSVAQISPLNYFKGAHGISSVTSVSIYGSSSSNIELHSTGGDGCICYLEYDKFEQKMDFIGMRQVKELSLVKSFFPNENPDYAIGFASADFIIWNLSTETKVAEISCGGWRRPHTFFLGDIPEMKNCFAFVKDEVIYMHKHWVVASDKIYPQNLHLQFHGREMHSLCFIVDRTNSIPLAFIATGCEDGTVRLTRYSSGVDNWSASKLLGEHVGGSAVRSLCSVQKVHTFTCHTMSTAGAIIKQGTDLDDQEDQFLLISVGAKRVITAWKRKSTSTPKSEMLFQWLSSDLPNRKGGTNLNGSKKEETNRNLDNVSIDKPPPENSEVCCNDGKENDWRYLAVTAFLVKISGSRTSVCFIVVSCSDATVTLRALVLPHRLWFDIALLIPSASPVLSLQHVIIPELPSFEDKSQTKSVFTVISGSTDGSIAIWDVTETVQTFMQKVSNLKKEDCRNFQRRPRTGRGSQGGRQWRSLDNSDKETVATLPKPSENTKDDMQDQTSLNVKDNESCFSCNVISGGDDQALHSFCFNVMASDRPTSETHFPRHVKTFGNYQIMFSHPDKIASAHSSAVKGVWTDGHWVFSTGLDQRLRCWRVSTDGKVSEHSHLIVSVPEPEALDVRKYGRNRYQIAVAGRGMQMMEFIPPVEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CZV9</accession>
    <name>A0AAD5CZV9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_006343</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005917">
        <property type="protein sequence ID" value="KAI7751411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CZV9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7751411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="62" mass="7385" checksum="A34F6216038EEA38" modified="2024-05-29" version="1" fragment="single">MCHRWIRHGGIRVDRSTEMVSKRLWYPYNRSRLAYLESGIWNLTTPSATLKMVWYGILLAQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D174</accession>
    <name>A0AAD5D174_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">NADH:flavin oxidoreductase/NADH oxidase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_030728</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NADH:flavin oxidoreductase/NADH oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006186">
        <property type="protein sequence ID" value="KAI7750231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D174"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02933">
        <property type="entry name" value="OYE_like_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000073">
        <property type="entry name" value="12-oxophytodienoate reductase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001155">
        <property type="entry name" value="OxRdtase_FMN_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045247">
        <property type="entry name" value="Oye-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22893">
        <property type="entry name" value="NADH OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22893:SF91">
        <property type="entry name" value="NADPH DEHYDROGENASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00724">
        <property type="entry name" value="Oxidored_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0288">FMN</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADH:flavin oxidoreductase/NADH oxidase N-terminal" evidence="8">
        <location>
            <begin position="7"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="113"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="113"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7750231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="364" mass="40446" checksum="553FFC1D74F6EDBE" modified="2024-05-29" version="1">MADKIPLLTPYKMGNFELSHRVVLAPLTRQRSFGNVPQPHAILYYSQRATKGGFLIAEATGVSDTAQGYPETPGIWTKQQVEAWKPIVDGVHEKGGIFFCQIWHVGRVSNTGFQPNGQAPISSTDKGLTYETRGDGNDASPPRKLTTEEIPHVVNDFRAAARNAIDAGFDGVEIHGAHGYLIDQFMKDGVNDRTDEYGGSLENRCRFPLEVVDAVVKEIGADKVGIRLSPFANYMQSGDSNPRALGLYMAESLNKHKILYCHMVEPRMKYLGEKVDSLHSLVPMRKAFKGTFISAGGYAMEDGNKAVAENRTDLVAYGRLFLANPDLPKRFELNAPLNKYNRETFYTPDPVLGYTDYPFLETMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D196</accession>
    <name>A0AAD5D196_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">signal peptidase I</fullName>
            <ecNumber evidence="5">3.4.21.89</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_020401</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins.</text>
            <dbReference type="EC" id="3.4.21.89"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Plastid</location>
            <location evidence="2">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase S26 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.89" evidence="5"/>
    <dbReference type="EMBL" id="JAMZMK010005745">
        <property type="protein sequence ID" value="KAI7752153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D196"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009003">
        <property type="term" value="F:signal peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010027">
        <property type="term" value="P:thylakoid membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06530">
        <property type="entry name" value="S26_SPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.109.10:FF:000012">
        <property type="entry name" value="Peptidase/ serine-type peptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.109.10">
        <property type="entry name" value="Umud Fragment, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036286">
        <property type="entry name" value="LexA/Signal_pep-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000223">
        <property type="entry name" value="Pept_S26A_signal_pept_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019758">
        <property type="entry name" value="Pept_S26A_signal_pept_1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019533">
        <property type="entry name" value="Peptidase_S26"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02227">
        <property type="entry name" value="sigpep_I_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43390">
        <property type="entry name" value="SIGNAL PEPTIDASE I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43390:SF2">
        <property type="entry name" value="THYLAKOIDAL PROCESSING PEPTIDASE 2, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10502">
        <property type="entry name" value="Peptidase_S26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00727">
        <property type="entry name" value="LEADERPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51306">
        <property type="entry name" value="LexA/Signal peptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00761">
        <property type="entry name" value="SPASE_I_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0934">Plastid</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Peptidase S26" evidence="12">
        <location>
            <begin position="41"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="68"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR600223-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF10502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7752153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="181" mass="20166" checksum="5FF0EAD4624548A2" modified="2024-05-29" version="1">MVKDDKDKEEKKVVSESVKRCEVVVKSGWFNKVLNVCSDDAKAAFTALSVSILFRSQLAEPRSIPSASMSPTLDVGDRILAEKVSYVFRRPEVSDIVIFKAPPILQKYGYSSGDVFIKRIVAKAGDCVEVRGGKLLINGVAQDEEFILEPLAYEMEPMVVPEGYVFVLGDNRNNSFDSHNW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D2E5</accession>
    <name>A0AAD5D2E5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Disease resistance protein At4g27190-like leucine-rich repeats domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_000070</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005584">
        <property type="protein sequence ID" value="KAI7752911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D2E5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057135">
        <property type="entry name" value="At4g27190-like_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050905">
        <property type="entry name" value="Plant_NBS-LRR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463">
        <property type="entry name" value="NB-ARC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33463:SF198">
        <property type="entry name" value="RPP4C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23247">
        <property type="entry name" value="LRR_RPS2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0611">Plant defense</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Disease resistance protein At4g27190-like leucine-rich repeats" evidence="2">
        <location>
            <begin position="52"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance protein At4g27190-like leucine-rich repeats" evidence="2">
        <location>
            <begin position="177"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7752911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="263" mass="30453" checksum="D2F7E4C9CE1C6B61" modified="2024-05-29" version="1" fragment="single">MDSQSSRQLAAIQPQQLLPYLYLERIHLEYLKKMSHVWKCNWNKFLIPQHQPLQFPFQNLTDIYVEQCDKIKYLFSPLMAKYLSNLKTVNLEWCRDIEEVISSRDDENTTSTSSHQNTTFFPHFDTLKLKDLPGLKRIDGEEFTWSRRDKISSEITNIIQDDLQSAQVTASYWSLCQYARKISIYNCHTLSSLIPWYAVGQTKRLQELKIEKCSGMTEVFENELMNNNTNNVDKGSGGATSLTSLPLKNSTTVVVPQLTNLNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D2R8</accession>
    <name>A0AAD5D2R8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Mitochondrial import inner membrane translocase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_014814</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">The twin CX3C motif contains 4 conserved Cys residues that form 2 disulfide bonds in the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small Tim family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005705">
        <property type="protein sequence ID" value="KAI7752327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D2R8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.810">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit tim13 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004217">
        <property type="entry name" value="Tim10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035427">
        <property type="entry name" value="Tim10-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02953">
        <property type="entry name" value="zf-Tim10_DDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144122">
        <property type="entry name" value="Tim10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="1" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="1" id="KW-0653">Protein transport</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0811">Translocation</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Tim10-like" evidence="2">
        <location>
            <begin position="14"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU367043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7752327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="115" mass="13389" checksum="9FBF4D80A82FF154" modified="2024-05-29" version="1" fragment="single">MDPSALNNPQLQQLINQEKERAMANEMIAKLTSACWDKCITGTPGSKFSSSESNCLSNCAQRYMDMSMMIVKRDKDTFHMLARFTREAKESSYIRKKTKTMYTNIYYRKKQDPTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D7D5</accession>
    <name>A0AAD5D7D5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PCI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_000074</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005584">
        <property type="protein sequence ID" value="KAI7752915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D7D5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050756">
        <property type="entry name" value="CSN3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057985">
        <property type="entry name" value="TPR_PSMD3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10758:SF2">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10758">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 3/COP9 SIGNALOSOME COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25573">
        <property type="entry name" value="TPR_PSMD3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="1">
        <location>
            <begin position="116"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7752915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="292" mass="32560" checksum="B47E66509A96E34B" modified="2024-05-29" version="1" fragment="single">MTQDIEMKEQQSPSNSTSSTVPVLQHLKEIASLIETGAYAREVRRISRAVRLTIALRKKLKGSVISSFLGFALAPGSEANQRLSSYLSKDDQNDMEVDTALSGATVKHSPELEIYCYLLVLIFLIDQKKYNEAKACSSASIVRLKNLNRRTVDVLASRLYFFYSLSYELTGELSEIRGNLLALHRVATLRHDELGQAVRIGDLELFKTVAERFSTTFSSDRTNNLIVRLRHNVIRTGLRNISISYSRISLADVAKKLRLDSPNPVADAESIVSKAIRDGAIDATLDHANGWM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D7R5</accession>
    <name>A0AAD5D7R5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_005314</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010002740">
        <property type="protein sequence ID" value="KAI7754695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D7R5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005875">
        <property type="term" value="C:microtubule associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051231">
        <property type="term" value="P:spindle elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000236">
        <property type="entry name" value="Kinesin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969">
        <property type="entry name" value="CHROMOSOME-ASSOCIATED KINESIN KIF4A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969:SF12">
        <property type="entry name" value="PLUS-END-DIRECTED KINESIN ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25764">
        <property type="entry name" value="KIF21A_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="3 5" id="KW-0505">Motor protein</keyword>
    <keyword evidence="1 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="8">
        <location>
            <begin position="1"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="36"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="577"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="796"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="375"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="747"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="37"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7754695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="923" mass="105066" checksum="27B355B4C33F71B7" modified="2024-05-29" version="1">MNALFNKIESLKDQIEFQLHVSFIEILKEEVRDLLDPNSSNKSETPNGQTGKVNIPGKPPIQIRENSNGVITLAGSTECSVQTLKEMADCLEHGSLSRATGSTNMNNQSSRSHAIFTITVEQIRKTNSSDNSLNESMGDEYLCAKLHLVDLAGSERAKRTGSDGMRFKEGVHINKGLLALGNVISALGDEKKRKEGAHVPYRDSKLTRLLQDSLGGNSRTVMIACVSPADINAEETLNTLKYANRARNIQNKPVVNRDPMSSEMLRMRQQLECLQAELCARGGGSPVELQALRERISWLEATNQDLCRELHLYRSRSIAIHHSEMNDNQVDDSFCMENEVLRKSVDSSDYQMSENGDSAVIDEAAAKEWEHTLLQDSMDKELHELNKRLEQKESEMKLVGGYDTMTLKQHFGKKIMELEEEKREVQKERDRLQTEIENLSSTSDGQTQKLQDLHAHKLKSLESQIQDLKKKKDSQVMVTKQKQKSDEAAKKLQDEIQYIKAQKVQLQHRIKQEAEQFRQWKASREKELLQLKKEGRRNEYERHKLQALNQRQKMVLQRKTEEAAMATKRLKELLEARKSTRDNSATGNGNGNGTNVQSNEKSLQRWLDHEVEVMVNVHEVRHEYEKQSQVRAALAEELAVLRQVDEYAAKGMSPPRGKNGFSRASSLSPEARMSRIASLESMLSISSNSLVLMATQLSEAEERERAFANRGRWNQLRSMADAKNLLQYMFNSLADARCQSWEKDIEMKEMEEQLQELVGLLSQSELRRKEVEKELKVREQQTVAIALASSAAGNSHNSLKHLADEKSGPLSPNSVPVPKQLKYTAGIVNGSVRESAAFTDPKRKMVPMSQLSMKKLGQLALAGHSSGKLWRWKRSHHQWLLQFKWKWQKPWRLSELIKHSDETIMRSKPRPLPAGSETMYHRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D856</accession>
    <name>A0AAD5D856_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phospho-N-acetylmuramoyl-pentapeptide-transferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_004970</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005117">
        <property type="protein sequence ID" value="KAI7753906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D856"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016780">
        <property type="term" value="F:phosphotransferase activity, for other substituted phosphate groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044038">
        <property type="term" value="P:cell wall macromolecule biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000715">
        <property type="entry name" value="Glycosyl_transferase_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22926">
        <property type="entry name" value="PHOSPHO-N-ACETYLMURAMOYL-PENTAPEPTIDE-TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22926:SF5">
        <property type="entry name" value="PHOSPHO-N-ACETYLMURAMOYL-PENTAPEPTIDE-TRANSFERASE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00953">
        <property type="entry name" value="Glycos_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="39"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="65"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="89"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="169"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7753906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="193" mass="20080" checksum="DF75AA97AF7B7441" modified="2024-05-29" version="1">MVVPLPLPLGLVCMGGSYVLLTSLCFVSMANGMELTDGLDGLAGGTAALAFIGMSIAVLPICSDLAVFGASMAGACVGFLFHNRYKASVFMGDTGSLALGGALAAMAASTGMFFPLLISSGVIVLEALSVLLQVSYFKTTKHMHGIGRRLFRMIPLHYHLESCGIKEPVIVAGAYMISCILMLYAGYVGLISV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-02-05" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D8T3</accession>
    <name>A0AAD5D8T3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_006711</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010004039">
        <property type="protein sequence ID" value="KAI7754236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000013">
        <property type="entry name" value="TBC1 domain family member 8B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000018">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF20">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="3">
        <location>
            <begin position="259"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="122"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="214"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="621"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="140"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="228"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7754236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="738" mass="84603" checksum="17AA50FCD8E51365" modified="2024-05-29" version="1">MNDEAKTSVWEVELVSFVKYVVILAEDAYGFTVRPQYLETFKEFDRIYKNEEEERSTKWRIFLQQHSEVGQTNTSIEEDVTNADADVTEHETKHVLETIEEEDVTKADADVTENETEHVLETIEEDNVDVSSDEKPTNDDQTENAPEEVVSEATEAVSEATEVVSEATKIIKPVVQNWAEVRSSLNTIENMMCHRVKNVPKSSPVEHLQTIQEVGPTTEDSNEELNDSKQPNSSGGSSAEVNPESLFPWKELESLVRGGVPRDLRGEAWQAFVGVKERRVXXXXXXXXXXXXXKVERAHVPEQCKKQIEKDLPRTFPGHPALNEEGRNSLRRLLLAYARHNPDVGYCQAMNFFAAMLLLMMPEENAFWTLVGIIDNYFDGYFTTDMIESQVDQLVFEDLMRERYPNLVDHFDRMGVEMGWICGPWFLSIFVNMIPWESVLRVWDIILFEGNRAMLLRTALALMELYGPDVGSTMDAGDAITLLQSLVGSTFDSSYLIVTACISFSYVTEDTLQTLRQKHRPEVIAAVQERMKGEHLRKSSKGLATKLYSFKKEREPIVRELNTKQGLNEKGSDKDSLDLMSPLKRDMSFKMEPITEVDSLSDLQDQVIWLKAQLCSMLEDKRSATVRAEELETALMEMAQQDNRREMSARIEDMEHEVSELEQLLADKKEQEKAMLEVLMRVEQEYKIAEDARRSAERDAAIQRHLVRVLELFYQKFVNQVNLCLISVGNFIYTLLHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D926</accession>
    <name>A0AAD5D926_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">1-aminocyclopropane-1-carboxylate synthase</fullName>
            <ecNumber evidence="9">4.4.1.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_023889</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>S-adenosyl-L-methionine = 1-aminocyclopropane-1-carboxylate + S-methyl-5'-thioadenosine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21744"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:58360"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="4.4.1.14"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="8">Alkene biosynthesis; ethylene biosynthesis via S-adenosyl-L-methionine; ethylene from S-adenosyl-L-methionine: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.4.1.14" evidence="9"/>
    <dbReference type="EMBL" id="JAMZMK010001674">
        <property type="protein sequence ID" value="KAI7755197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016847">
        <property type="term" value="F:1-aminocyclopropane-1-carboxylate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009693">
        <property type="term" value="P:ethylene biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009835">
        <property type="term" value="P:fruit ripening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000051">
        <property type="entry name" value="1-aminocyclopropane-1-carboxylate synthase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050478">
        <property type="entry name" value="Ethylene_sulfur-biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43795:SF10">
        <property type="entry name" value="1-AMINOCYCLOPROPANE-1-CARBOXYLATE SYNTHASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43795">
        <property type="entry name" value="BIFUNCTIONAL ASPARTATE AMINOTRANSFERASE AND GLUTAMATE/ASPARTATE-PREPHENATE AMINOTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00753">
        <property type="entry name" value="ACCSYNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0266">Ethylene biosynthesis</keyword>
    <keyword evidence="7" id="KW-0292">Fruit ripening</keyword>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="11">
        <location>
            <begin position="32"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00033478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7755197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="387" mass="43430" checksum="A7CBDC7526E26833" modified="2024-05-29" version="1">MQLSRTGFLVLPRMGRVREESXXXXXXXKGIIQMGLAENQLSFDLLESWLEKNPQPMSFKNHSNQSIFKDLALFQDYHGLPAFKNSLVKFMSEIRGGSVSFDPKNLVLTAGATSANETLMFCLANPGDAFLLPTPYYPGFDRDLKWRTGAEIVPIQCSSLNGFRITKSALEEAYKQAQKHNLKVKGILVTNPSNPLGTSLTLHELDLLVNFISSKNIHLISDEIYSGTVFSSPCFISIMEVLKNKNLMNTEIAKQVHIVYSLSKDLGLPGFRIGAIYSNDEKVVSAATKMSSFGLVSSQTQYLLSEILSDQKFTKTYLSENQRRLKERHDMLVKGLRKSGIACLPSNSGLFCWVDMSHLLSSNTFEGEMELWKKIVYEDNSTKQITF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D9J4</accession>
    <name>A0AAD5D9J4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Kinesin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_015902</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010001117">
        <property type="protein sequence ID" value="KAI7755437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D9J4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005876">
        <property type="term" value="C:spindle microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008574">
        <property type="term" value="F:plus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090307">
        <property type="term" value="P:mitotic spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051231">
        <property type="term" value="P:spindle elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047149">
        <property type="entry name" value="KIF11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47970:SF30">
        <property type="entry name" value="KINESIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47970">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIF11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3 5" id="KW-0505">Motor protein</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="7">
        <location>
            <begin position="57"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="20"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="36"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="142"/>
            <end position="149"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7755437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="214" mass="23600" checksum="A6DA190F4B4DBAE4" modified="2024-05-29" version="1" fragment="single">MASFRNGSRGPSKVDRVQPAGSTNLRASASFKSKTSNVRRSSSSSNVRDSVDSVSGRVRVAVRLRPQNAEEMVADADFADCVELQPEFKRLKLRKNNWDSDTYEFDEVLTESASQKRVYEVVAKPVVESVLDGYNGTVMAYGQTGTGKTFTLGRLGDEDTSARGIMVRAMEDILANISRDSDSVTVSYLQLYMETIQDLLNPVNDNIAIVEDPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D9X0</accession>
    <name>A0AAD5D9X0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_007351</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000957">
        <property type="protein sequence ID" value="KAI7755644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D9X0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004560">
        <property type="term" value="F:alpha-L-fucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000028">
        <property type="entry name" value="Alpha-L-fucosidase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049053">
        <property type="entry name" value="AFCA-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016518">
        <property type="entry name" value="Alpha-L-fucosidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054363">
        <property type="entry name" value="GH95_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027414">
        <property type="entry name" value="GH95_N_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31084:SF16">
        <property type="entry name" value="ALPHA-L-FUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31084">
        <property type="entry name" value="ALPHA-L-FUCOSIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14498">
        <property type="entry name" value="Glyco_hyd_65N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21307">
        <property type="entry name" value="Glyco_hydro_95_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22124">
        <property type="entry name" value="Glyco_hydro_95_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF007663">
        <property type="entry name" value="UCP007663"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyl hydrolase family 95 N-terminal" evidence="1">
        <location>
            <begin position="44"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 95 catalytic" evidence="3">
        <location>
            <begin position="301"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha fucosidase A-like C-terminal" evidence="2">
        <location>
            <begin position="721"/>
            <end position="778"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF21307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7755644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="816" mass="91805" checksum="7E9A78C1E02006DD" modified="2024-05-29" version="1">MIKREIQGWRHCKLEHRPYKHAANCVIVKMQAVMVEKDNLKVRFKGEAKHWTDALPIGNGRLGGMVWGGVLSETINLNDDTLWTGRPGNYTNPDAPKALSEVRKLVSDGKYAEATTSAVKLSGEASNAYQLLGDINLKFHETDAKYNETTYRRELDLDTATVKVMYSIGEVEFTRQHFVSHPDQVMVSKFSGNKSGSLSFTVFLDSKMPHRASVNAQNQIVMEKTGDGIKFSAVLDLRTSNGIGTISVLEDNKIKVEGCDSVVILLAAYSSFAGPFINPADSKNDPTIDCLNNLKHLKGYSYEQLYTRHLDDYHKLFHRVSINLSKSSILTDSYVATSERVKSFKTDEDPLLVELLFQYGRYLLISCSRPGTQPANLQGIWNDKVYPPWDGAPHLNINLQMNYWHSLSCNLHECQEPLFDFIKNLSVNGSKTAKVNYEAKGWVAHQVSDIWAKTSPDRGEAVWALWPMGGAWLCTHLWEHYTYTMNKEFLAKIAYPLLEGCVLFLLDWLIEGQDKYLQTNPSTSPEHMFTAPNGQPASVSYSTTMDMSIIKEVFSAIVSAAQVLGKSEDDLIKKVVESQSKFYPTHIAKDGSIMEWAQDFVDPDVHHRHISHLFGLFPGHTITLKETPELCKAAEVTLNKRGVEGPGWSTTWKVASWARLHNSEHAYQMIKHLFDLVDPENESKYEGGLYSNLFTAHPPFQIDANFGFSAALAEMLIQGSVTELYVLPALPRDKWRSGSVEGLKARGNVTVSISWSDGDLHKLSLWSDDRKSQRFTKTIHYKEMSIVATMVTRKVYTFDGKLKAIKTAPLLDSSKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DAH5</accession>
    <name>A0AAD5DAH5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ubiquitin-like 1-activating enzyme E1A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_023296</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein sumoylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Heterodimer of SAE1A or SAE1B and SAE2. The complex binds SUMO proteins via SAE2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ubiquitin-activating E1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000694">
        <property type="protein sequence ID" value="KAI7755925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DAH5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031510">
        <property type="term" value="C:SUMO activating enzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019948">
        <property type="term" value="F:SUMO activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000404">
        <property type="entry name" value="SUMO-activating enzyme subunit 1B-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF162">
        <property type="entry name" value="SUMO-ACTIVATING ENZYME SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="THIF-type NAD/FAD binding fold" evidence="10">
        <location>
            <begin position="14"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00063459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7755925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="319" mass="35424" checksum="FF46A9DACD285459" modified="2024-05-29" version="1">MNGEELTEQETALYDRQIRVWGVDAQRRLSKSHVFVSGLKGTAVEFCKNIVLAGVGSLTLNDDNPVTEEALSANFLIPFDENMGGGGSLAGLCCDCLKDFNPMVHVSVEKGDLSSFDVDFFQKFDVVVISCCSLAAKKSVNEKCRKLSKRVAFYTVDCRDSCGEIFVDLQKYAYAKKKADETVECQLQYSSFEEAIAIPWRSLPKRVSKLYFAMRVVERFEEVERRKPGETSIEDLPKVLKLRNELCLAHSLNESQIPDSLLERLVVSTKEFPPVSAVLGGILGQEVIKAISGKGDPLKNFFFFDAVDGKGLIEDISTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DAS2</accession>
    <name>A0AAD5DAS2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">L-ascorbate oxidase homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_026500</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the multicopper oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000643">
        <property type="protein sequence ID" value="KAI7756004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DAS2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001117">
        <property type="entry name" value="Cu-oxidase_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF27">
        <property type="entry name" value="OS01G0816700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00394">
        <property type="entry name" value="Cu-oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041938052" description="L-ascorbate oxidase homolog" evidence="3">
        <location>
            <begin position="20"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="6">
        <location>
            <begin position="30"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="4">
        <location>
            <begin position="156"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="5">
        <location>
            <begin position="382"/>
            <end position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7756004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="552" mass="62096" checksum="5B17352CA118F862" modified="2024-05-29" version="1">MDARTFGVFTLLLITLANAEDPYHFFEWKVTYGTMSPLGIPQQVILINGKFPGPVINCTSNNNIVVNVFNNLDEPFLLTWNGVQQRKNSWVDGTPGTMCPIQPGKNFTYKFQVKDQIGSYYYFPTTALHKAAGGMGIIQIHSRPLIPVPFDNPAEEFAVLVGDWFNKGHKSMKNILDSGKSIGRPDGLHINGKSAQVGAKAEPSWTFEAGKTYRFRICNVGMRTSVNFRFQGHDLKLVELEGSHVMQNEYLSMDLHVGQCMSVLVTADQPPKDYYLVVSSRFTKQPHTIVATVRYKGSNSPASPELPPPPPGNTAGIAWSLNQFRSYRWNLTASAARPNPQGSYHYGKINITRTIKLVNSRNYIDGKLRFALNGVSHLDPKTPLKLSEYFGVADKEFKYNLIADEPPPDVEKNIKIAPNVINATYRNFVEIIFENHEKTIQTYHLDGYSFFAVAVEPGKWSPDKRKNYNLEDALSRHNVAVFPGCWAAVMLTLDNAGMWSLRSEMWERFYLGQQLYVSVLSPERSLRDEYSLPDTTQLCGIVASMPKPKGYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DCL0</accession>
    <name>A0AAD5DCL0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Oxidation resistance protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_027920</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the OXR1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000238">
        <property type="protein sequence ID" value="KAI7756885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DCL0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006571">
        <property type="entry name" value="TLDc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354:SF62">
        <property type="entry name" value="MUSTARD, ISOFORM V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354">
        <property type="entry name" value="NUCLEOLAR PROTEIN 7/ESTROGEN RECEPTOR COACTIVATOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07534">
        <property type="entry name" value="TLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00584">
        <property type="entry name" value="TLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51886">
        <property type="entry name" value="TLDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TLDc" evidence="6">
        <location>
            <begin position="203"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="36"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="155"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="43"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="64"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="164"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="188"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00040604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7756885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="388" mass="42519" checksum="BAA0B8C800082D4F" modified="2024-05-29" version="1">MKLGKQQQQQQERQRLTTSIRSKAAHFVSDLTTVILNPISDKPSSPSSGNGSNEPRRNTQQCSQEDDVPVLEDGPDTSSFTAFLYSFLSPGRSEETENEYANWNGNQSVATNKHDSSSANALIKENSGKKGIFSRGKQSIGRALTQAARYGTGYRFHSSGKVNTESKVDDGKETPGSQVGANEGVLAQNPSGPKLPNTSEPSQLMTEDTRAALYVALPVLSQGKKWVLLYSTWRHGISLSTLYRRSNLCPGLSLLVVGDRKGAVFGGLVEAPLRPSTKKRYQGSNDTFVFTNTPGCPVIFRPTGINRYFTLCSTEYLALGGGNHFALYLDHDLLNGSSLASETYGNSCLSHTQDFEVKEIELWGFVYASEYEETISMLRTEAPGICRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G1Q4</accession>
    <name>A0AAD5G1Q4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_017946</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011977">
        <property type="protein sequence ID" value="KAI7725305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G1Q4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000084">
        <property type="entry name" value="Wall-associated receptor kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045274">
        <property type="entry name" value="WAK-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27005:SF492">
        <property type="entry name" value="LOW QUALITY PROTEIN: WALL-ASSOCIATED RECEPTOR KINASE-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27005">
        <property type="entry name" value="WALL-ASSOCIATED RECEPTOR KINASE-LIKE 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="85"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00047558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7725305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="366" mass="41448" checksum="FE2EA8726153689C" modified="2024-05-29" version="1">MFKKSNDNVFIPHTGIDIGSFMPKKPYLGMNLASLTPKRWKQLTPREKFFQKNGALLLEDKLKSGVGSIKLFQAEELERATMNFAESMIIERGGGNGIVYKGILSDNRVVAIKRSRTLDEEQVEPFMNEIVVLGQINHRNVVQLLGCCLETEVPLLVCEFVSNYNLYHHIHQNTSDNGKFPWDSRLRIAHESAGALAYLHSDATTTIIHRDVKSANILLDENYTSKVADFGASKSLPLGHDQVFTVVYGTLGYMDPEYMTSGQLTDKSDVYSFGVVLAELLTGLKPIDEEDTNLATFFVQAKRENRLREIVDRQVLEEATDAQLDAVCNLVCRCLDREGRNRPSMKEVTMELERLRKHKSFNVYHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G216</accession>
    <name>A0AAD5G216_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RNase III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_004154</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011811">
        <property type="protein sequence ID" value="KAI7725949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G216"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004525">
        <property type="term" value="F:ribonuclease III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030422">
        <property type="term" value="P:siRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00593">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1520.10:FF:000004">
        <property type="entry name" value="Endoribonuclease dicer-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1520.10">
        <property type="entry name" value="Ribonuclease III domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014720">
        <property type="entry name" value="dsRBD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000999">
        <property type="entry name" value="RNase_III_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036389">
        <property type="entry name" value="RNase_III_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14950">
        <property type="entry name" value="DICER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14950:SF53">
        <property type="entry name" value="RIBONUCLEASE 3-LIKE PROTEIN 3 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00035">
        <property type="entry name" value="dsrm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00636">
        <property type="entry name" value="Ribonuclease_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00535">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69065">
        <property type="entry name" value="RNase III domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00517">
        <property type="entry name" value="RNASE_3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50142">
        <property type="entry name" value="RNASE_3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RNase III" evidence="9">
        <location>
            <begin position="72"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7725949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="309" mass="35793" checksum="A8F2A1B8DF76550C" modified="2024-05-29" version="1">MELHLHLQKKMLCKAYGTEHRYSMAPSSSWWRSLFSPSNNPFTLLHCFKRLQDYTNLKLVTSSQEKKALASLKEMEKIIGYKFNKKLLLQQALTHPSYQESESYERLEYLGDSILNFLISKQQFFMYPNLSPGSLTALRAANVDTEKLARVAVKYNFHKYLRHENPVLSKQIQVFIKAIEKYPFHSYGLIDAPKTLADVVESTIGAIYVDSNSSIDTTWEVAKILLEPMITPEMLQQNPVRKLNELCHKRKLKIRLRDKWLKQGIYEVFIDNQLIGKGVYKAKKEIALNRAAEDACNNILNNISESLDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G4B5</accession>
    <name>A0AAD5G4B5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_012162</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SINA (Seven in absentia) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="JAMZMK010011456">
        <property type="protein sequence ID" value="KAI7727061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G4B5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16571">
        <property type="entry name" value="RING-HC_SIAHs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03829">
        <property type="entry name" value="Sina"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000041">
        <property type="entry name" value="E3 ubiquitin-protein ligase SINAT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000483">
        <property type="entry name" value="E3 ubiquitin-protein ligase SINAT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.210.10:FF:000004">
        <property type="entry name" value="E3 ubiquitin-protein ligase SINAT5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.210.10">
        <property type="entry name" value="Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018121">
        <property type="entry name" value="7-in-absentia-prot_TRAF-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052088">
        <property type="entry name" value="E3_ubiquitin-ligase_SINA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049548">
        <property type="entry name" value="Sina-like_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008974">
        <property type="entry name" value="TRAF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013010">
        <property type="entry name" value="Znf_SIAH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10315">
        <property type="entry name" value="E3 UBIQUITIN PROTEIN LIGASE SIAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10315:SF164">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21362">
        <property type="entry name" value="Sina_RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03145">
        <property type="entry name" value="Sina_TRAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21361">
        <property type="entry name" value="Sina_ZnF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51081">
        <property type="entry name" value="ZF_SIAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="11">
        <location>
            <begin position="60"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="SIAH-type" evidence="12">
        <location>
            <begin position="113"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7727061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="322" mass="36497" checksum="D0A94DFE40E96AD9" modified="2024-05-29" version="1">MMDLSTIECASSSDGLEDEEIHSQITHQFVSKPHNNNNSNVVGIPPGLPPATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTQVQNRCPTCRQELGNIRCLALEKVAESLELPCRYFSLGCPEIFPYYSKLKHEALCNFRPYNCPYAGSECSVTGDIPFLVTHLRDDHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDEADARNYSYSLEVGANGRKLTWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSGGDRKELKLRVTGKIWKEQQHPEGGVCIPNLCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G5D8</accession>
    <name>A0AAD5G5D8_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_006358</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011258">
        <property type="protein sequence ID" value="KAI7728186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G5D8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7728186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="78" mass="9069" checksum="CDBDF2A26CD2E820" modified="2024-05-29" version="1">MRGGASPFPNRILSYCREDVWANNDNSRIRSCVYTTARKLSLHSLAIWLHAILYIKLCFDCKECFITVHHNWDLLAAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G652</accession>
    <name>A0AAD5G652_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glycosyltransferase</fullName>
            <ecNumber evidence="5">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_016320</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="5"/>
    <dbReference type="EMBL" id="JAMZMK010010805">
        <property type="protein sequence ID" value="KAI7730315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G652"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080043">
        <property type="term" value="F:quercetin 3-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080044">
        <property type="term" value="F:quercetin 7-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000027">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000065">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058980">
        <property type="entry name" value="Glyco_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926:SF1557">
        <property type="entry name" value="7-DEOXYLOGANETIN GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926">
        <property type="entry name" value="GLUCOSYL/GLUCURONOSYL TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26168">
        <property type="entry name" value="Glyco_transf_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyltransferase N-terminal" evidence="6">
        <location>
            <begin position="13"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF26168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7730315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="480" mass="53056" checksum="9DB3C4D4B0C23878" modified="2024-05-29" version="1">MASNQEHKPHALFIPAPAQGHLNPMIKLAKILHSKGFLITFVNTEFTHRRLLKSQGSAALNSVSSFRFETIPDGLPPPENTEVTQNVYDICRSTQDTCLQPFKSLVAKLSDTFSPVTCIVSDLLMGFTLDAAKELGIPEFIFSTGGAGALLCSEQFPTLLDKGLMPLKDPSYVVNGYLDTIVDSIPGMKGIPLKYIPPFIRYINPGDEFMVEFTNAQIKKAKTASGIIFNTFDDLEHDVLDSLASIFPPCYGIGPLHLQEHNIADKSLASIGSNLWIDEPECIKWLDTQAASSVVYVNFGSITVMTHQQLVEFCWGLANSKHPFLWIMRAGLVDGESEALPQEFFKETSGRGMLAGWCPQEQVINHPSVGGFLTHCGWNSTVESISSGVPMICWPFFSDQFPNCWLSCTKWGVAMEIDSDVKRDEVERLVIELMAGEKGMVMRKNAMNWKDKANAACSVPNGSSVVDLEKLVHQLKTTRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G8W0</accession>
    <name>A0AAD5G8W0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_025259</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010399">
        <property type="protein sequence ID" value="KAI7731726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G8W0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002">
        <property type="entry name" value="RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE SD1-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002:SF932">
        <property type="entry name" value="RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="48"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7731726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="189" mass="20974" checksum="2B63F7B14E15424F" modified="2024-05-29" version="1" fragment="single">KQNEDEVGFSFTNTLKSIKGSFTEKINGENVDFHVFDLSTLTTATDNFSHLNKLGVGGFGTVYKGKLLNGQEIAVKRLSQSSVQGMQEFRNEVWDLWKQEKPLLAVDSSLGDTFNAQEVLLCIHVGILCVQELAVDRPTMTDVASMLSNHETVLPSPNQPAFIFKQLNHGRDHDVGSVNDEDITILHAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GA75</accession>
    <name>A0AAD5GA75_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_010791</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009745">
        <property type="protein sequence ID" value="KAI7734367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GA75"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46519">
        <property type="entry name" value="RING/U-BOX SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46519:SF3">
        <property type="entry name" value="RING_U-BOX SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7734367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="130" mass="15233" checksum="56A9E36A5D9D670A" modified="2024-05-29" version="1">MARWDWTLGQILLIHSVKLWILPKLLFGMDLWACLNLRRTKNSVTKTCENNGKQDGLGVDQTTVGARRSVRLRYGRYALLDLLTRFEKERKKEMQSLLESRPVSAFAHRKRIQEFISVTLPHCRKLQLPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GB23</accession>
    <name>A0AAD5GB23_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Amino acid transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_031191</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009410">
        <property type="protein sequence ID" value="KAI7735925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GB23"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48017">
        <property type="entry name" value="OS05G0424000 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="46"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="87"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="128"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="161"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="202"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="235"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="278"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="317"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="343"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="376"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="8">
        <location>
            <begin position="32"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7735925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="407" mass="44703" checksum="546F7014DDA9BADA" modified="2024-05-29" version="1" fragment="single">IRSVKKKKKLPPSFSIDWIYLHRLIDRFHTKGVGILSIPYALASGGWVSLILLFLIAGSTFYTGLLIKRCMDLDPTIRNYPDIGDRAFGKTGGTIVSVIVNIELYLVATGFLILEGDNMSNLFPNMDFYIYGTHIAAKTGFVILVAIIILPTSWLNNLSILSYISASGVLATVIILSSVFWAGAFDGIGFEEKGTFLNWSGIPSAISLYAFCYCAHPVFPTLYTSMRNQHQFSKVLFVCFAFCTVTYSLMAIIGYLMFGSNAESQITLNLPTHHISSRVAICTTLVSPIAKYALMLTPTVNAIEARFQSYNKRKCSFLIRTILMISTVIVALSLPFFGYLMSLVGALLSATTSITIPCLCYLKISGIYRRIGVEVVVVGFVVLSGLIVAVVGTYVSLIDIINHLGVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GBI8</accession>
    <name>A0AAD5GBI8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Bet v I/Major latex protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_033015</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BetVI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009488">
        <property type="protein sequence ID" value="KAI7735414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GBI8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010427">
        <property type="term" value="F:abscisic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004864">
        <property type="term" value="F:protein phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009738">
        <property type="term" value="P:abscisic acid-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009820">
        <property type="term" value="P:alkaloid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07816">
        <property type="entry name" value="Bet_v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000916">
        <property type="entry name" value="Bet_v_I/MLP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050279">
        <property type="entry name" value="Plant_def-hormone_signal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31213:SF19">
        <property type="entry name" value="BET V I_MAJOR LATEX PROTEIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31213">
        <property type="entry name" value="OS08G0374000 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00407">
        <property type="entry name" value="Bet_v_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0017">Alkaloid metabolism</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bet v I/Major latex protein" evidence="3">
        <location>
            <begin position="3"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7735414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="155" mass="16985" checksum="093B0F74BFC976FF" modified="2024-05-29" version="1">MFGTLSEEAEVKVPASKAWALYGTLEIGKLVVGKVLEAVDVVEGDGGLGTILKLTFKPGSGFPYYKEKFTKVDNENMVKETEVVEGGFLDIGFTLYRIRFEIKDNPNDDTGSSCLQKLTIEYDVKEEFAANASLVTIEPLLGIMSVANEHLLKSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GC22</accession>
    <name>A0AAD5GC22_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_028051</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009236">
        <property type="protein sequence ID" value="KAI7736572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GC22"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7736572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="176" mass="19103" checksum="2EF6CFBA2D9724E0" modified="2024-05-29" version="1">MCRFHLPPPYAGPMQPITNLEKILMNMWINKFPTQDSILNAQKGSEIFGQHGWTCGKEKGMVVSGVKTLSAVVWDLYGRDSGVIGQALCGLPGNYQDQKMGVATLVWPLWAQKPIERLMNTVEAKIYWAIYGQVFGNGGCNSRQTCASGEVEDVDSSYVDPGIVVASGPGCKACGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GCT0</accession>
    <name>A0AAD5GCT0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_002783</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Mediates both low-affinity uptake and efflux of sugar across the plasma membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SWEET sugar transporter family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008999">
        <property type="protein sequence ID" value="KAI7737532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GCT0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051119">
        <property type="term" value="F:sugar transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.290:FF:000002">
        <property type="entry name" value="Bidirectional sugar transporter SWEET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047664">
        <property type="entry name" value="SWEET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004316">
        <property type="entry name" value="SWEET_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10791">
        <property type="entry name" value="RAG1-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10791:SF30">
        <property type="entry name" value="SUGAR TRANSPORTER SWEET1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03083">
        <property type="entry name" value="MtN3_slv"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="16"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="50"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="77"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="110"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="138"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7737532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="183" mass="20143" checksum="231672B89C1D1B35" modified="2024-05-29" version="1">MLWIFYGLPFNHPNSTLVVTINGTGLVLELIYLSVFLLYGKSSHRKKITFCLIVEVIFLGLVAGLDFRFFHTHKSRSAFVGAFCVVFGVAMYASPLTIMYKVIKTKSVEFMPFPLSLAAFLNGICWTTYALLKFDLNILIANGIGAVLGAVQLVLYGCYLKTTPKKSKTSAAAANGEVQLQNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GFB9</accession>
    <name>A0AAD5GFB9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_024378</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008837">
        <property type="protein sequence ID" value="KAI7738281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GFB9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7738281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="74" mass="8504" checksum="9AA3765E5FB6DF63" modified="2024-05-29" version="1" fragment="single">MAGRQAADYHRNRQSAAKTPLPVTVLMKSWPEQLLRCDQVFYHFPLYVHVFGAKRVFPDTLTGPRHTAPVPTRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GFC7</accession>
    <name>A0AAD5GFC7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Transcription factor CBF/NF-Y/archaeal histone domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_009423</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008454">
        <property type="protein sequence ID" value="KAI7740350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GFC7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016798">
        <property type="term" value="F:hydrolase activity, acting on glycosyl bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003958">
        <property type="entry name" value="CBFA_NFYB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044255">
        <property type="entry name" value="Dr1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47173">
        <property type="entry name" value="PROTEIN DR1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47173:SF2">
        <property type="entry name" value="PROTEIN DR1 HOMOLOG ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00808">
        <property type="entry name" value="CBFD_NFYB_HMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042045635" description="Transcription factor CBF/NF-Y/archaeal histone domain-containing protein" evidence="1">
        <location>
            <begin position="16"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription factor CBF/NF-Y/archaeal histone" evidence="2">
        <location>
            <begin position="92"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7740350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="219" mass="24820" checksum="734DC1752C0AAF4C" modified="2024-05-29" version="1">MVVMFFFVDFIAAIAKVMNRFSKKSDSCQGRVLEDTSVSLSMIGGSIPVYWYELEFKLDRKASSRLLQSYLFECMVNLLLVTEFKRLWSVEPLPTMTKIIKEMLSPDVHVARDTQDLLIECCVEFINLVSSKSNEVCGIEDRRTIAPGHGANNWSGRGCQIGVHKSMGDGKILPKYGNVFASATQRTQTWNGIQQDITTRVERKLAYNVIARVRILLQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GFK5</accession>
    <name>A0AAD5GFK5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Disease resistance protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_019498</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008390">
        <property type="protein sequence ID" value="KAI7740620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GFK5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005744">
        <property type="term" value="C:TIM23 mitochondrial import inner membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013261">
        <property type="entry name" value="Tim21"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766:SF61">
        <property type="entry name" value="NB-ARC DOMAIN DISEASE RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36766">
        <property type="entry name" value="PLANT BROAD-SPECTRUM MILDEW RESISTANCE PROTEIN RPW8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08294">
        <property type="entry name" value="TIM21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0611">Plant defense</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="318"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7740620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="457" mass="51210" checksum="1979E672A4BAAC56" modified="2024-05-29" version="1" fragment="single">ELSIQGMDAIKVIGLECTSTASVTFPALEILKFEVEELSIEHCDEIRHLWESNVEASKVSSCKKLVSLGEKEEDEDNIGSNLLSSLRKLVIRSCESMERLCCPNSIESLHINGYNSIKQVSFPGATTTDCESIESFGNLQLPNLTDLDIISCKNMKEIRDLQIPNLISWSIIGCKNLESIPEVELCNLTLLRDMRITSCPMTDASFPGGIWPSNLQSLEIGGLKKPISEWGYQNFPASLVKLVLYNGGEVRNLSELSHLFPSSLTSLNIYAFKNLESFSMGLQHLTSLQHLQIWECPEVKDLPETLLPTLLSLTIEGASYSVVILAGLGIAAAASYGVFKELIFEPKKYKVFNKALRRIQDESQVRVRMGHRLPDTVKKAEIEQPDNGSLIEFGKTMMVLSMLRFNFISEAHMERVGFLQRCLKTKMTNNGSLCICKLKSTLHLHQECYLNHIFQPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GG77</accession>
    <name>A0AAD5GG77_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">WAT1-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_018557</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the drug/metabolite transporter (DMT) superfamily. Plant drug/metabolite exporter (P-DME) (TC 2.A.7.4) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008493">
        <property type="protein sequence ID" value="KAI7740184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GG77"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030184">
        <property type="entry name" value="WAT1-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31218">
        <property type="entry name" value="WAT1-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="68"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="93"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="131"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="186"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="218"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="250"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="280"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="306"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="8">
        <location>
            <begin position="5"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="8">
        <location>
            <begin position="187"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="336"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="348"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7740184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="397" mass="44311" checksum="3B0B8A02A18AF86A" modified="2024-05-29" version="1">MQRLKAILMMVAAQFAVAAFSISYKLASYDGMHMRVLITYRYLFASILIFPLALFLERNKRPKLTWSILFQAFLCAIFGGPMSQNMYAESLVLTSATFTVAFTNLIPPLTFIIAVLFRLEKVEIRKRKGKAKVIGTLIGVAGAMILTFYKGHQLNIKSTHFNFLHGGQHMDEHVATTNNLSTHDHIFGSILALGYSISIALFYIFLSKLSVNYPCHYSNTFLYSVIGFLQSLVCVLPTERSWSKWKLNSSMRLFSAIFQGICAVLGLFLTTTVVHLEGPLFASIFNPLVLVFVGLADFLILGEKLYVGSLIGSIIVIVGLYLVLWGKGKETKTLSKSVSSANSKDKNSSLNTTSRTTTLKTTHMPHTSLWFDVDKHDELPADSSMENTKVKEILEEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GGE5</accession>
    <name>A0AAD5GGE5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pyridine nucleotide-disulfide oxidoreductase domain-containing protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_010734</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Probable oxidoreductase that may play a role as regulator of mitochondrial function.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Interacts with COX5B; this interaction may contribute to localize PYROXD2 to the inner face of the inner mitochondrial membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the carotenoid/retinoid oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008628">
        <property type="protein sequence ID" value="KAI7739391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GGE5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10668">
        <property type="entry name" value="PHYTOENE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10668:SF103">
        <property type="entry name" value="PYRIDINE NUCLEOTIDE-DISULFIDE OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13450">
        <property type="entry name" value="NAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amine oxidase" evidence="6">
        <location>
            <begin position="212"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00037217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00040298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7739391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="560" mass="61044" checksum="A29EA983B3EC6C6F" modified="2024-05-29" version="1">MWRRSFCTTTAATLATKKWDALIIGGGHNGLTAAAYLAQSGLSVAVLERRHVIGGAAVTEELIPGFKFSRCSYLQSLLRPSIIRDLELHRHGLKLLKRNPSSFTPCLDGRYLLLGSDAEMNRNEISKFSERDADAYPRYEDQLEKFCKFMDPLIDSSPPEAHQGVSSFNSRLKHKLQKSEFWAHCLHQSVSMGQQGLVDFMDLLLSPASKVLNNWFESEVLKATLATDAVIGTTTSAHTPGSGYVLLHHVMGETDGHRGIWSYVEGGMGSVSLAIGKAAKEAGATIVTDAEVSKMMIEDSDKVTGVMLADGTKIHSSVVLSNATAYKTFVELVPENVLPDDFSRAVKHSDYSSATTKINLAVDKLPQFRCCNIGYPEAGPQHMGTIHIGSESVEEIETACQEARNGLPSRRPVIEMTIPSVLDKTISPPGKHVINLFVQFTPYKPTDGSWEDTAYREAFAKRCFNLIDEYAPGFSSSVIGYDMLTPPDLERIIGLTGGNIFHGAMGLDSLFLMRPVKGWSNYRTPLRGLYLCGSGAHPGGGVMGAPGRNAARVVIDDIKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GIG8</accession>
    <name>A0AAD5GIG8_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_022915</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007992">
        <property type="protein sequence ID" value="KAI7742434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GIG8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008479">
        <property type="entry name" value="DUF760"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33598">
        <property type="entry name" value="OS02G0833400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33598:SF10">
        <property type="entry name" value="SEED MATURATION-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05542">
        <property type="entry name" value="DUF760"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7742434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="359" mass="41001" checksum="C75850232C1B7618" modified="2024-05-29" version="1">MAAPSSRPLLSHRLTNPHHKPLPHPYSHHLLFRPFLPNLSTASTSNNNRSNYYSTINCRISSENGDVSDEFLSTNSFYREFSVIANMLKQIEPLDTSVISKGVSDSVKESMNRMISSMLGLLPSDQFAVRISVSKRPLVRLLASSVITGYTLWNAEYRVTLMRNFETSTKRLNSGNCDDATKDQSEEECFSEELERLKLESCLGDLSSDAMNYIQELESELSTAKKDLHDQKQETTQIGHARESNNDILKYLRSLDPDMLDELSRPSSSEVKRILQQLVQCASRRVFKEDITSGLMGDAEVVQENHKNGDNFCETRDHLAKLLFWSSVKRFGEQILASYDHQSLQHIQMYKKNLYQTKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GJ01</accession>
    <name>A0AAD5GJ01_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_010400</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007852">
        <property type="protein sequence ID" value="KAI7742929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GJ01"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7742929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="141" mass="15321" checksum="6E294D0F25DAD08C" modified="2024-05-29" version="1">MATRPTFPATRPTFPNESMTIALAAHESNIDIWVLLSSVSSVVEFWRLERLDLTYNNLASLEVDVGFFLLCAMTSTDPAGDGDGCRHPKWDGCDMSGTGVGGNVGWSSCRCRFHLNSVEEAATLLLGVERVNTACQYGISD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-02-05" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GKN4</accession>
    <name>A0AAD5GKN4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Transmembrane 9 superfamily member</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_015518</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the major facilitator superfamily. Phosphate:H(+) symporter (TC 2.A.1.9) family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the nonaspanin (TM9SF) (TC 9.A.2) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006967">
        <property type="protein sequence ID" value="KAI7746470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072657">
        <property type="term" value="P:protein localization to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004240">
        <property type="entry name" value="EMP70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10766:SF55">
        <property type="entry name" value="TRANSMEMBRANE 9 SUPERFAMILY MEMBER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10766">
        <property type="entry name" value="TRANSMEMBRANE 9 SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02990">
        <property type="entry name" value="EMP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="290"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="356"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="389"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="423"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="460"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="510"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="542"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="577"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="621"/>
            <end position="647"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7746470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="653" mass="74182" checksum="79A6E80186FBF07B" modified="2024-05-29" version="1">MDFFDKFKIWVLSICLISQLGHGFYLPGSYPHKYVVGDQLSVKVNSLTSIETEIPYSYYSLPFCHPPEGIKDSRENLGELLMGDRIENSPYKFNMYTNETEVFLCETKPLSGEEYKMLVNRIDEMYQVNVILDNLPAIRYTKRDGYYVRWTGYPIGIKVDNGYYLFNHLKFTVLVHKYEETNVASVMGTGDGADVIPTVGKARSDVPGYMVVGFEVTPCSVRHDLEAVKNLKTYGKYPSKITCEDNTVNMAIKENEPVAFTYEVAFVESDIKWPSRWDAYLKMEGAKVHWFSILNSLMVITFLAGIVLVIFLRTVRRDLTHYEELDKEAQAQMNEELSGWKLVVADVFRAPSNPGLLCIMVGNGVQILGMAVVTIMFAALGFMSPASRGTLVTGMLIFYMVLGILAGYVAVRMWRTIFCGDHRGWVSVSWXXXXIAFLILFTLNFLLWGSHSTGAIPFSLFVILILLWFCISVPLTFVGGYFGAKAPHIEYPVRTNQIPREIPPQKYPSWLLVLGAGTLPFGTLFIELFFIMSSIWMGRVYYVFGFLFVVLILLVVVCAEVSLVLTYMHLCVEDWKWWWKSFFASGSVALYIFLYSINYLIFDLKSLSGPVSATLYLGYSFLMVLAIMLATGTVGFLSSFWFVHYLFSSVKLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GKX4</accession>
    <name>A0AAD5GKX4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">65-kDa microtubule-associated protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_031544</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MAP65/ASE1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007620">
        <property type="protein sequence ID" value="KAI7743966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GKX4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000911">
        <property type="term" value="P:cytokinesis by cell plate formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1520:FF:000002">
        <property type="entry name" value="65-kDa microtubule-associated protein 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1520">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007145">
        <property type="entry name" value="MAP65_Ase1_PRC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19321:SF41">
        <property type="entry name" value="FASCETTO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19321">
        <property type="entry name" value="PROTEIN REGULATOR OF CYTOKINESIS 1 PRC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03999">
        <property type="entry name" value="MAP65_ASE1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="391"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="460"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7743966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="469" mass="52900" checksum="CF0B88C8F141BAAD" modified="2024-05-29" version="1">MAEPTCGSLLLQLQKIWDEVGESDEERDKALLQIEQECLNVYKRKVEQAAKSKEHLLQTLEDAKLEFSTLLKSLGEKSVLNISQRLKKVLEFVSNLHDICGVLGIDFYRTVSDVHPSLIDASSAQSKSISNDTLARLAKSVIALEEAKKQRLHRLQELAAQLTELWNLMDTSEEERSLFNHVTCNISASVDEVTVPGALALNLIEQAEVEVERLDKLKASKMKEIALKKQEELEEIFAHVHIQNDTKAAREKILALIDSGNVEPAELLADMDAQVIKAKEEALSRKDILDKVEKWMSACEEESWLEDYNRDNKRYSGGRGAHLNLKRAEKARILVSKIPGIVDTLVAKTQTWEEEHGITFAYDGVPLLDMLNEYSVLRHDREEEKRRIRDQKKYHEQLNTGQEAMFGTKPSPARPLSSSKKMTGPNGAPNRRLSLNQNGTRSVKRDHTRPVAPLNYVAISKEDPAAVTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GLE8</accession>
    <name>A0AAD5GLE8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">PHD finger protein ALFIN-LIKE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_017206</name>
        <name evidence="15" type="ORF">M8C21_017208</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">AA19_3_7</strain>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Histone-binding component that specifically recognizes H3 tails trimethylated on 'Lys-4' (H3K4me3), which mark transcription start sites of virtually all active genes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Interacts with H3K4me3 and to a lesser extent with H3K4me2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">The PHD-type zinc finger mediates the binding to H3K4me3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the Alfin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007495">
        <property type="protein sequence ID" value="KAI7744579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAMZMK010007495">
        <property type="protein sequence ID" value="KAI7744581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GLE8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd15613">
        <property type="entry name" value="PHD_AL_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000306">
        <property type="entry name" value="PHD finger alfin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045104">
        <property type="entry name" value="Alfin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021998">
        <property type="entry name" value="Alfin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044104">
        <property type="entry name" value="PHD_AL_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12321">
        <property type="entry name" value="CPG BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12321:SF178">
        <property type="entry name" value="PHD FINGER PROTEIN ALFIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12165">
        <property type="entry name" value="Alfin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="3 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0804">Transcription</keyword>
    <keyword evidence="7 11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="13">
        <location>
            <begin position="185"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="143"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU369089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7744579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAI7744581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="241" mass="27361" checksum="330C7715DB3CF768" modified="2024-05-29" version="1">MEMAAISSSPRTVEEIFKDYSARRSGIVRALTYDVDEFYATCDPEKENLCLYGHPNETWEVTLPAEEVPPELPEPALGINFARDGMHRRDWLSLVAVHTDSWLLAVAFYFGARLNRNERKRLFSMINELPTVFEVVTERKHVKDKPVDNGNKSRSSTKRTSDGQAKSTPRAYDESYAEEEDEHGETLCGTCGGNYSGDEFWIGCDICERWYHGKCVKITPAKAESIKQYKCPSCSTKKGRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GLF9</accession>
    <name>A0AAD5GLF9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RSE1/DDB1/CPSF1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_032019</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DDB1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007631">
        <property type="protein sequence ID" value="KAI7743908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.910:FF:000003">
        <property type="entry name" value="DNA damage-binding protein 1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000239">
        <property type="entry name" value="DNA damage-binding protein 1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050358">
        <property type="entry name" value="RSE1/DDB1/CFT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004871">
        <property type="entry name" value="RSE1/DDB1/CPSF1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10644">
        <property type="entry name" value="DNA REPAIR/RNA PROCESSING CPSF FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03178">
        <property type="entry name" value="CPSF_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RSE1/DDB1/CPSF1 C-terminal" evidence="4">
        <location>
            <begin position="78"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7743908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="421" mass="47874" checksum="B8CA1553C05E241B" modified="2024-05-29" version="1" fragment="single">XXXXMCPFNSAAFPDSLAIAKEGELTIGTIDDIQKLHIRSIPLGEHARRICHQEKSRTFAICSLKYNQSSTEESEMHFIRLLDDQTFDFISTYSLDQFEYGCSILSCSFSDDNNAYYCVGTAYVMPEENEPSKGRILVFIVEDGKLQLVAEKETKGAVYSLNAFNGKLLAAINQKIQLYKWMLRDDGTRELQSECGHHGHILALYVQTRGDFIVVGDLMKSISLLIYKHEEGAIEERARDYNANWMSAVEILDDDIYLGAENNFNLFTVRKNSEGATDEERGRLEVVGEYHLGEFVNRFRHGSLVMRLPDSDVGHIPTIIFGTVNGVIGVIASLPQDQYIFMEKLQTSLRKVIKGVGGLSHEQWRSFYNEKKTADSRNFLDGDLIESFLDLGRNRMEEISKTMNVSVEELMKRVEELTRLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GLG9</accession>
    <name>A0AAD5GLG9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_027832</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006803">
        <property type="protein sequence ID" value="KAI7747265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GLG9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7747265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="110" mass="12644" checksum="E5BDB99EC9566675" modified="2024-05-29" version="1">MQMKEGNEDKVCKHGWRPDLVVVCLLHDIFFWHNHMNVGIDQTPVTIFKRTATRAAKIRYPVLIFSDLIPVNPCSYFDGNVNGQMRIDIDSSDKHVLEPSYPLDLIQFAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GNA2</accession>
    <name>A0AAD5GNA2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_027831</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the peroxisomal membrane protein PXMP2/4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006803">
        <property type="protein sequence ID" value="KAI7747264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GNA2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007248">
        <property type="entry name" value="Mpv17_PMP22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266:SF106">
        <property type="entry name" value="PEROXISOMAL MEMBRANE 22 KDA (MPV17_PMP22) FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 2, PXMP2 MPV17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04117">
        <property type="entry name" value="Mpv17_PMP22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="123"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="166"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7747264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="188" mass="21658" checksum="9BEE8919BAA3E934" modified="2024-05-29" version="1" fragment="single">MNATFHKLSNQTYNHIRTRCSIPNTIHRRRTHVQRNLHLPNPFCPRFFLPIRRRFCSKTNGTTPSASFTNSGPIWWYLSMIKARPILTKSLTSAIIYTAADLTSQTITKSSSEAYDFIRTCRMAGFGMTIFGPIMTALFFSVNAGLQGERVQEIMARLKRDMLPTMISNAIYWPLCDFLTFRFIPVHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GPJ3</accession>
    <name>A0AAD5GPJ3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_033754</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006155">
        <property type="protein sequence ID" value="KAI7750430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GPJ3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18919">
        <property type="entry name" value="bHLH_AtBPE_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000002">
        <property type="entry name" value="Basic helix-loop-helix transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024097">
        <property type="entry name" value="bHLH_ZIP_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12565:SF184">
        <property type="entry name" value="BHLH TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12565">
        <property type="entry name" value="STEROL REGULATORY ELEMENT-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="5">
        <location>
            <begin position="146"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7750430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="331" mass="37790" checksum="79F8B18FE395433F" modified="2024-05-29" version="1">MLHCPYTAMNIPGNTDMSVFERQQARIKLQQQQQQQQELFNGNGHQFSGRVEVKPDPGMQYGWPDFSDVCQDQMISPLVVDNNIKKRKTLEDEKQKVVVKENSVKEKKIKMCSEDINSAETSSKEKQKIAEAPKQDYIHVRARRGQATDSHSLAERVRREKISERMKYLQDLVPGCNKITGKAGMLDEIINYVQSLQKQVEFLSMKLATLQPRLDFDIDSLICMKEMLEVPAVAYSSEVANSTYFQVNSLLEMGNINTIDAMLRRSIGGPVSIPETFVVSSCFNQMHPGVTWDGDLHNLYRMEIQQESSTIPFQPHKFTGSYEGNNLKMEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GQJ1</accession>
    <name>A0AAD5GQJ1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ABC transmembrane type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_031160</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006445">
        <property type="protein sequence ID" value="KAI7748916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GQJ1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18579">
        <property type="entry name" value="ABC_6TM_ABCC_D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044746">
        <property type="entry name" value="ABCC_6TM_D1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF375">
        <property type="entry name" value="ABC TRANSPORTER C FAMILY MEMBER 11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="99"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="9">
        <location>
            <begin position="24"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7748916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="194" mass="22226" checksum="99C08AC87A4F3454" modified="2024-05-29" version="1" fragment="single">MHMRQHTMMMVLLIVEGEGLKKSLGVLCEAQYFQNVARVGFRIRSILVSAVFRKSLRLTHEARKNFPSGKTMNMITTDANALQQVCNQLHGLWSAPFRIVLSMILLYQQLGIASLVGSLVLILMFPIQTMIVSKMRKLSKEGLQYTDKRVGLMSEILAAIQTVKCYAWEESFKSKIQTIRKDELLWLWKSQFLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GQW8</accession>
    <name>A0AAD5GQW8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Dynein light chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_017179</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Cytoplasm</location>
            <location evidence="2 10">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the dynein light chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006244">
        <property type="protein sequence ID" value="KAI7749964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GQW8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005868">
        <property type="term" value="C:cytoplasmic dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.740.10:FF:000005">
        <property type="entry name" value="Dynein light chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.740.10">
        <property type="entry name" value="Protein Inhibitor Of Neuronal Nitric Oxide Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037177">
        <property type="entry name" value="DLC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001372">
        <property type="entry name" value="Dynein_light_chain_typ-1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11886">
        <property type="entry name" value="DYNEIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11886:SF39">
        <property type="entry name" value="DYNEIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01221">
        <property type="entry name" value="Dynein_light"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01375">
        <property type="entry name" value="Dynein_light"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54648">
        <property type="entry name" value="DLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-0243">Dynein</keyword>
    <keyword evidence="5 10" id="KW-0493">Microtubule</keyword>
    <keyword evidence="10" id="KW-0505">Motor protein</keyword>
    <keyword evidence="6" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU365010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7749964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="96" mass="10652" checksum="AA805ACF04F5C3F7" modified="2024-05-29" version="1">MAISMQNNAFKCSTEYLHSIPPGNGNGNVKKIDSKRLALALKKKFDCLYGPAWHCIVGTSFGSYVTHSSGCFLYFSIDKVHVLLFKTTVDPVDIQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GR23</accession>
    <name>A0AAD5GR23_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FHA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_008172</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006197">
        <property type="protein sequence ID" value="KAI7750144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GR23"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022212">
        <property type="entry name" value="DUF3741"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032795">
        <property type="entry name" value="DUF3741-assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025486">
        <property type="entry name" value="DUF4378"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46634">
        <property type="entry name" value="M REDUCTASE II SUBUNIT GAMMA, PUTATIVE (DUF3741)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46634:SF3">
        <property type="entry name" value="M REDUCTASE II SUBUNIT GAMMA, PUTATIVE (DUF3741)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12552">
        <property type="entry name" value="DUF3741"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14309">
        <property type="entry name" value="DUF4378"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14383">
        <property type="entry name" value="VARLMGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FHA" evidence="2">
        <location>
            <begin position="20"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="295"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="448"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="525"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="653"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="308"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="448"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7750144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="855" mass="95723" checksum="304B435FD003EBC5" modified="2024-05-29" version="1">MSWIQHGKKQSPEKPISGCLGRMVNLFDLNTSVGGNKLLTDTPYYDGSSFSRRHSDVARTSLVDDHMDDKLIVSDLRKSPSNRRLNGTPIKMLIAQEMSKEEDSKQSPSNLVAKLMGLDALPHQHQHDSASRMSQSGSLEFLQQEQGEYKDVYEIRQNDSPQKGKCSEKQKMALVREKFMEAKRLSSDEKLRQTKQFQDALEVLSSNKDMFLKYLQEPNSLFSQNIYNLQSVPPPPESRRITILKPSKLVDDEKPDWNKKGSDILFSPECCKSDDAPNLPTRIVILKPSSVKPQNIKVVSSSPSNSTSHDDVFDGHTEETADELSESLSGLRRDETLVSSVFSNGYIGDDSSYCKSEIDYATGNLSDSEVVSPASRHSWDYINRYESHYGASSSRTSYSPESSVCREAKKRLSERWAMISSNKNVVEQRQIQRSSSTLGDMLALSDFKKPVKPEENHETSDFGKAEDTGRGSRNLLRSNSVPARLGLEDSGKLKDKVDDTKEAVKEKIVKSSSFKGRVSSLFFSKNKKSSKEKSQQSNDIPQSTRFSPKHGTKGSQCSGDMVTEEASCSGPKKGLFLPEAGFSFTKPDFPGNHIANPDQPSPISVLEQQFEEDDQTTNCHHSLRLNKYGMEPMRYNLIDKSPPIGSIARTLSWDDSTGSATPYHGKPSSTPLNPEEEEQECLSYVQTLLSAAGLSQIQSNSILGKWHSPESPLDPSLRDKYTNLSEKEPTTGQTKLRHHRASSKLVFDCVNEALIDISARGPYSGAHNNIVDKTMSSAMLEDHVWGQMKEWLWREEMCDWEEGDVGGSMVVVKEVVGRGWVEGLRMEVVNTRKEIERKLLEELVQECVPELTSRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GT55</accession>
    <name>A0AAD5GT55_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">Target of rapamycin complex subunit LST8</fullName>
            <shortName evidence="6">TORC subunit LST8</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Lethal with SEC13 protein 8 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_008899</name>
        <name evidence="7" type="ORF">M8C21_032193</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of TORC1 complex, which is an essential cell growth regulator that controls plant development. Acts by activating transcription, protein synthesis and ribosome biogenesis, and inhibiting mRNA degradation and autophagy.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">The target of rapamycin complex 1 (TORC1) is composed of at least RAPTOR, LST8 and TOR.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the WD repeat LST8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008067">
        <property type="protein sequence ID" value="KAI7742148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAMZMK010004112">
        <property type="protein sequence ID" value="KAI7754210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GT55"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031931">
        <property type="term" value="C:TORC1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031932">
        <property type="term" value="C:TORC2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032956">
        <property type="term" value="P:regulation of actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031929">
        <property type="term" value="P:TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000179">
        <property type="entry name" value="Target of rapamycin complex subunit LST8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037588">
        <property type="entry name" value="MLST8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19842">
        <property type="entry name" value="G BETA-LIKE PROTEIN GBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19842:SF0">
        <property type="entry name" value="TARGET OF RAPAMYCIN COMPLEX SUBUNIT LST8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 5 6" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="74"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="115"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="207"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="253"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU369068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7742148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7754210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="316" mass="35272" checksum="99D5433C1703EDDF" modified="2024-05-29" version="1">MTQPSVILATASYDHTIRFWEAKSGRCYRTIQYPESQVNRLEITPDKRFLAAAGNPHIRLFDVNSTSPQPVMSYDSHTNNVMAVGFQCDGKWMYSGSEDGTVKIWDLRAPGCQREYESRAAVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELVPEVDTAVRSLTVMWDGSLVVAANNKGTCYVWRLLRGTQTMTNFEPLHKLQAHDGYILKCLLSPEFCEPQRYLATASSDSTVKIWNVDGFTLEKTLVGHQRWVWDCVFSVDGAYLITASSDTTARLWSMSTGEDIRVYQGHHKATVCCALHDGAETTGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GTF8</accession>
    <name>A0AAD5GTF8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phosphatidylinositol 4-phosphate 5-kinase</fullName>
            <ecNumber evidence="6">2.7.1.68</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_024582</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14425"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58178"/>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.68"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.68" evidence="6"/>
    <dbReference type="EMBL" id="JAMZMK010003072">
        <property type="protein sequence ID" value="KAI7754540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GTF8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016308">
        <property type="term" value="F:1-phosphatidylinositol-4-phosphate 5-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17302">
        <property type="entry name" value="PIPKc_AtPIP5K_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.810.10">
        <property type="entry name" value="2-Layer Sandwich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.110.10">
        <property type="entry name" value="Histone H3 K4-specific methyltransferase SET7/9 N-terminal domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.800.10">
        <property type="entry name" value="Phosphatidylinositol Phosphate Kinase II Beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017163">
        <property type="entry name" value="PIno-4-P-5_kinase_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027483">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002498">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027484">
        <property type="entry name" value="PInositol-4-P-5-kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023610">
        <property type="entry name" value="PInositol-4/5-P-5/4-kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23086:SF113">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4-PHOSPHATE 5-KINASE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23086">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01504">
        <property type="entry name" value="PIP5K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037274">
        <property type="entry name" value="PIP5K_plant_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00330">
        <property type="entry name" value="PIPKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82185">
        <property type="entry name" value="Histone H3 K4-specific methyltransferase SET7/9 N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51455">
        <property type="entry name" value="PIPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 6 7" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 6 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PIPK" evidence="9">
        <location>
            <begin position="340"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="279"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR037274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7754540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="724" mass="82304" checksum="D98908EFF9C16D89" modified="2024-05-29" version="1">MSKDLGGFLKSWEGKVKKSRAVIMFTTMSVAHVDEECCDSEAVPVHVEKIFSNGDIYMGEGMAENCPHGSGKYLWADGCMYCGEWCKGMMNGRGKFSWPSGATYEGQFKNCYMDGEGTFTGSLNHTYKGCWVMNRRHGKGSMSYANGDHYEGDWRKGVYDGVGTYEWSSGHRYTGQWKRGKMSGNGVMVLANGNQFDGSWECGLPKGNGAFRFEDGSFYMGFWGKDQKEQLVKFYPSNSTVAHDDWDPNQLFSFMSECVVYAVESLSVFPSDKMFSWSSSDGKHKKQPVRMNSRNENKGRRRSIDAMGGLDDLENGHIRGLGINQQPTEKQGVTICKGHKNYELMLNLQLGIRHSVGRPSAPKPLDLKPITFNTKEKIWTRFPPEGSKHTPPHQSCDFKFKDYCPSIFRTLRNLFNVDPVDYMLSICGNEALRELSSPGKSGCFFYMTSDNKYMIKTMKKAEVKVLRRMLPAYFEHMKSFENTLVTKFFGLHCVKLSGSSQRKVRFVVMGNLFCTNVPINRRFDLKGSSHGRITDKPESEIDANTTLKDLDLNCIFRLRKDRFQEFYRQINKDCEFLEQERIMDYSLLVGISAQECNQDISAGSWNAELDSSGSSPRISSADTDASLDPTGWAHIRLGVNMPARAELTVRSHETQLVGEPTQEFYDVTLFFGIIDILQDYDISKKLEHAYKAFQYDPTSISAVDPIQYSKRFRDFIFSVFKEEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GU54</accession>
    <name>A0AAD5GU54_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_029471</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the major facilitator superfamily. Phosphate:H(+) symporter (TC 2.A.1.9) family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005471">
        <property type="protein sequence ID" value="KAI7753339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GU54"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015798">
        <property type="term" value="P:myo-inositol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015791">
        <property type="term" value="P:polyol transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17360">
        <property type="entry name" value="MFS_HMIT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000073">
        <property type="entry name" value="MFS myo-inositol transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050814">
        <property type="entry name" value="Myo-inositol_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020:SF39">
        <property type="entry name" value="MAJOR FACILITATOR, SUGAR TRANSPORTER, MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020">
        <property type="entry name" value="PROTON MYO-INOSITOL COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="26"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="67"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="101"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="124"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="157"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="188"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="270"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="308"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="339"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="372"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="406"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="439"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="30"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7753339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="492" mass="52737" checksum="EFF2BE8A8FB1C95B" modified="2024-05-29" version="1">MTIGLVPGSSGRIDDDKKITYFSNKYVLGLTVVAGIGGLLFGYDTGVISGALLYIRDDFEAVNQSSFLQETIVSMALLGAMIGAAAGGWLNDVQGRKSATLIADVIFAVGSFVMAAAPDPYVLIFGRLLVGLGVGIASVTAPMYIAEAAPSEIRGGLVSTNVLMITGGQFLSYLVNLGFTQVRGTWRWMLGIAAVPAILQFGLMLLLPESPRWLYKNRGSSEAVVTLSKIYDPDRLNDELQHLSNALEEERSGRNSVRYRDVFRIKEIRLAFLAGAGLQAFQQFTGINTVMYYSPTIVQMAGFGSNQLALQLSLIVALMNAAGTVVGIYLIDYVGRRKLALSSLTGVILSLVLLSVAFFLESSGNTSMGWVAVLGLVLYISSFAPGMGPVPWTVNSEIYPESYRGICGGMAATVNWVSNLIVAQSFLSVADAVGTGMTFSILAVIAVVAVAFVLIFVPETKGLTFEEVEKLWRIRARGNHNGAESLLEDNRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GUT6</accession>
    <name>A0AAD5GUT6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein DETOXIFICATION</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Multidrug and toxic compound extrusion protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_005245</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000385">
        <property type="protein sequence ID" value="KAI7756447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GUT6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13136">
        <property type="entry name" value="MATE_DinF_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044644">
        <property type="entry name" value="DinF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42893:SF46">
        <property type="entry name" value="PROTEIN DETOXIFICATION 44, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42893">
        <property type="entry name" value="PROTEIN DETOXIFICATION 44, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="207"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="250"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="283"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="408"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="447"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="480"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="506"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="38"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="38"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="68"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7756447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="546" mass="58655" checksum="5150EF1E809EBF94" modified="2024-05-29" version="1">MTTFVFQHHHPLLPSQIFKHNNNNNSFKLQSFRHIPNSSVRFRTSPKSSSRQNPNTSAPKPSVQNPQNDSEITSNDDNNNNSDSYSPSSSTSSNPLVLLVQQFREKLAFDELGMEIMSIALPAAVALAADPITSLVDTAFVGHLGSAELAAVGVSISIFNLVAKLLNIPLLNITTSFVAEEQAVLVEADDMVGRPNKKFLPSVSTSLAMATAFGIAETIALSFGSGFLLNAMGITVDSPMRVPAEQFTSIRAFGAPAIVLALAAQGTFRAAGNLLNAILDLVLIFFCGFGVDGAAIATVISEYLTAFILLWKLNEEVVLVTPNIDGEKVAQYLKSGALLMSRSFSVLVTMTLSTSVAAREGSVPMAGHQICLEVWLALSLLTDALALAGQAILAGSYSQRNYGEARRVIYRVLQIGLIAGCSLSFILLFEFEQLSYLISTDSEVLNIARSGTLFVVASQPVNAIAFVLDGLYYGVSDFGYASYSMVVIGLGTCAFFLLAGPKFGLAGVWAGLFVFMTLRVVAGIWRLGTKRGPWKLVYSETDQENR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GVQ7</accession>
    <name>A0AAD5GVQ7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Vesicle transport v-SNARE N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_027024</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Prevacuolar compartment membrane</location>
            <topology evidence="8">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the VTI1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010002276">
        <property type="protein sequence ID" value="KAI7754914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GVQ7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012507">
        <property type="term" value="C:ER to Golgi transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15862">
        <property type="entry name" value="SNARE_Vti1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.400:FF:000001">
        <property type="entry name" value="Vesicle transport through interaction with t-SNAREs homolog 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000002">
        <property type="entry name" value="Vesicle transport through interaction with t-SNAREsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.400">
        <property type="entry name" value="t-snare proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027027">
        <property type="entry name" value="GOSR2/Membrin/Bos1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010989">
        <property type="entry name" value="SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038407">
        <property type="entry name" value="v-SNARE_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007705">
        <property type="entry name" value="Vesicle_trsprt_v-SNARE_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21230:SF96">
        <property type="entry name" value="GOSR2_MEMBRIN_BOS1, VESICLE TRANSPORT V-SNARE DOMAIN SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21230">
        <property type="entry name" value="VESICLE TRANSPORT V-SNARE PROTEIN VTI1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05008">
        <property type="entry name" value="V-SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12352">
        <property type="entry name" value="V-SNARE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028865">
        <property type="entry name" value="Membrin-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47661">
        <property type="entry name" value="t-snare proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="176"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="Vesicle transport v-SNARE N-terminal" evidence="11">
        <location>
            <begin position="10"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="9"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00060376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF05008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7754914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="198" mass="22650" checksum="6941EE60E6163A41" modified="2024-05-29" version="1" fragment="single">YRKNSWNFLEQKKQKIAEVKAGIDEAEALIRKMDVEARNLPPNVKAVLLAKLREYKSDLNNLKSEIKRIASTNLNQAARDELLESGMADAAAVSADQRGRLLFSTERLNKSSDRVRDSRRTMLETEELGVSLLQDLHQQRQSLLHAHGTVHGVDENIGRSKKIMTNISRRMNRNKWIIGFMVTILVVAIILILYFKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GW20</accession>
    <name>A0AAD5GW20_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FBD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_006034</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010003865">
        <property type="protein sequence ID" value="KAI7754346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GW20"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006566">
        <property type="entry name" value="FBD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08387">
        <property type="entry name" value="FBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FBD" evidence="1">
        <location>
            <begin position="68"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7754346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="138" mass="15930" checksum="86CA59E8EDE52EA7" modified="2024-05-29" version="1">MLPHLRDLILFGPSFGKEDEIRFVLFLVTSSPNLKKLKIEMNYNPTEAISQTANSLFDLLDYSYVKLDHLDYLEITNFSNMKPEMDFLKLVLAKSPMLKKVRIVINNDVSATDEVKMLKDLLWNPCAYPGVQIKCERS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GW74</accession>
    <name>A0AAD5GW74_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">alanine--glyoxylate transaminase</fullName>
            <ecNumber evidence="5">2.6.1.44</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_027260</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.44" evidence="5"/>
    <dbReference type="EMBL" id="JAMZMK010000082">
        <property type="protein sequence ID" value="KAI7757705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GW74"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008453">
        <property type="term" value="F:alanine-glyoxylate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009436">
        <property type="term" value="P:glyoxylate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019481">
        <property type="term" value="P:L-alanine catabolic process, by transamination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049704">
        <property type="entry name" value="Aminotrans_3_PPA_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45688">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45688:SF3">
        <property type="entry name" value="ALANINE--GLYOXYLATE AMINOTRANSFERASE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8 11" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="13"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7757705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="465" mass="50749" checksum="8378ED8BB9A0CC46" modified="2024-05-29" version="1">MVVAVKGSLVGAETCSGDQGDREDGVVVEGSDEYNDGHRRPRRFSSLQPPELPSFDYEPVPYKGPSAQQVFEKRKKFLGPSLFHYYQKPLNIVEGKMQYLFDEDGKRYLDAFAGIVTVSCGHCHPEILRAIEEQNKLLQHATTIYLHHAIADFAEGLAEKMPGNLKVVYFVNSGTEANELAMLMARLYSGNLGMIALRNAYHGGSAGTIGLTALNTWKYPIPQGEIHHVVNPNPYRGVFGSDASLYAQDGVGGAVELAPGYLKMVYEMVRKAGGVCIADEVQTGFGRTGSHYWGFQTQDVIPDIVTMAKGIGNGLPLGAVVTTPEIAQVMAQKIQFNTFGGNPVCSAGGLAVLKVLDKENRQKHCADVGSHLITRLKNLQQKFDIIGDVRGRGLMVGIELVTDRKDKTPAKAETAVLFEKLRELGVLVGKGGLHGNVFRIKPPMCFNKDDADFLVDALDYSMTKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-02-05" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GWF3</accession>
    <name>A0AAD5GWF3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Nuclear transcription factor Y subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_018681</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Heterotrimeric transcription factor composed of three components, NF-YA, NF-YB and NF-YC. NF-YB and NF-YC must interact and dimerize for NF-YA association and DNA binding.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the NFYA/HAP2 subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000036">
        <property type="protein sequence ID" value="KAI7757960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016602">
        <property type="term" value="C:CCAAT-binding factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018362">
        <property type="entry name" value="CCAAT-binding_factor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001289">
        <property type="entry name" value="NFYA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12632">
        <property type="entry name" value="TRANSCRIPTION FACTOR NF-Y ALPHA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02045">
        <property type="entry name" value="CBFB_NFYA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00616">
        <property type="entry name" value="CCAATSUBUNTB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00521">
        <property type="entry name" value="CBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00686">
        <property type="entry name" value="NFYA_HAP2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51152">
        <property type="entry name" value="NFYA_HAP2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="3 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 8" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="107"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="143"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="157"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU367155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7757960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="171" mass="19522" checksum="D872800C2D439386" modified="2024-05-29" version="1">MKVISGKAQMIVRFSPHLLQLXXXXISHPSNAQYPTLGQAAYPYPDPYYRSLFTPYEAQPYPTHPYPVQPMLMGIQQGGVPLPSDAVEEPVFVNAKQYHGILRRRQCRAKAESENKARKSKKPYLHESRHLHALRRSRGCGGRFEKKDKQQKEGSDDNSQMKNINLNSDNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GYT7</accession>
    <name>A0AAD5GYT7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Eukaryotic translation initiation factor 3 subunit B</fullName>
            <shortName evidence="6">eIF3b</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">eIF-3-eta</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">eIF3 p110</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_018695</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the eIF-3 subunit B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000036">
        <property type="protein sequence ID" value="KAI7757974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GYT7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005852">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12278">
        <property type="entry name" value="RRM_eIF3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000286">
        <property type="entry name" value="Eukaryotic translation initiation factor 3 subunit B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000235">
        <property type="entry name" value="Eukaryotic translation initiation factor 3 subunit B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03001">
        <property type="entry name" value="eIF3b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011400">
        <property type="entry name" value="EIF3B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034363">
        <property type="entry name" value="eIF3B_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013979">
        <property type="entry name" value="TIF_beta_prop-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14068">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 EIF3 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14068:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08662">
        <property type="entry name" value="eIF2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036424">
        <property type="entry name" value="eIF3b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 6" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5 6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Translation initiation factor beta propellor-like" evidence="7">
        <location>
            <begin position="339"/>
            <end position="535"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7757974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="644" mass="74370" checksum="0802146370D568C6" modified="2024-05-29" version="1">MAAVMSMEDIRTEATRLNIDLSSVDWASVRLPPGEDCGIISDDDDLHEEDSLEFDAGFGNIIIVDNLPVVPREKFEKLEGVVRKIYSQIGVIKENGLWMPVDEDTGKTRGYCFIEYNTPQENLQRWLTDEKGRDQFVIRAGSDTEVLWNDARQVKADPVYKRPFWTESFVQWSPLGTYLATVHRQGAAVKLIDFSPGEKYLVTYSSHEPSNPRDTHRVVLNIFDVRTGKVMRDFKGSADEFAIGGTGGFSGVSWPVFKWGGGKEDKYFARMGKNVISVYDTETFGLIDKKSIKVDNVMDFSWSPTDPIFALFVPELGGGNQPARVSLFQIPSKVELRQKNLFSVSDCKMYWQSNGEYLAVKVDRYTKTKKSTYTGFELFRIKERDIPIEVFELDNKNDKVISFAWEPKGHRFAVIHGDNPRPDVSFYSVKGGKVLKLTTLKQKQANALFWSPSGRFIILAGLKGFNGQLEFYNVDELETMATAEHFMATDIEWDPTGRYVATSVTSVHEMENGFNIWSFNGKLLYRILKDHFFQFLWRPRPPSFLSPEKEEEIFKNLKKYSKKYDVQDQDISVLLSEQDREKRKQLKDDWERWVNEWKRYHEQEKMDRQMLRDGEASDVEEEYEAKEVEVEELLDVSEEIIQDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BMG4</accession>
    <name>A0AAD5BMG4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_005153</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012059">
        <property type="protein sequence ID" value="KAI7725121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BMG4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7725121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="72" mass="7867" checksum="A6D170F54B449D23" modified="2024-05-29" version="1">MWKDKSPGVKILWIWTIGTAAVLVANVATSRVRDMNKIINTPEQSPSPPNDLQTTDSIITGTVLDSSLEKES</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BN36</accession>
    <name>A0AAD5BN36_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_016952</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011676">
        <property type="protein sequence ID" value="KAI7726406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BN36"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009733">
        <property type="term" value="P:response to auxin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000001">
        <property type="entry name" value="MYB-related transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015495">
        <property type="entry name" value="Myb_TF_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10641">
        <property type="entry name" value="MYB FAMILY TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10641:SF1376">
        <property type="entry name" value="MYB-RELATED PROTEIN 306-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="HTH myb-type" evidence="7">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="6">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="7">
        <location>
            <begin position="39"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="6">
        <location>
            <begin position="39"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="100"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="193"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="193"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7726406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="304" mass="34385" checksum="9A72A43273268443" modified="2024-05-29" version="1">MLVSYVQQHGPGNWRQVPTNTGLRRCSKSCRLRWTNYLRPGIRRGDFTDDEEKMIIQLQALLGNKWAAIASYLPERTDNDIKNYWNTHLKKKLRKLQMGSDEHDLNHKKKDRFASSTTSSSSSHCISRGQWEKRLQTNIHLAKQALNDALSVDQKPILVPQSIKSPTQTPIYPSSTDNIAKLLKGFMKNTSNNNKTCSNASTRESSDEGNALSSESKSYSGIALTEAFESLFEFDRSFGSSNSSEFSQSNTSPEGSVFQGDSKDELRFMMFENWLLDDKTAVGVVDQGELSNFSFDENTDFFDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BPM4</accession>
    <name>A0AAD5BPM4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_033506</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011411">
        <property type="protein sequence ID" value="KAI7727207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BPM4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7727207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="29" mass="3157" checksum="B2A5E48BBB7E6DED" modified="2024-05-29" version="1" fragment="single">MGRENGMKVTCQVASKLNKSLISKSCDLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BQM7</accession>
    <name>A0AAD5BQM7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">M8C21_005989</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">AA19_3_7</strain>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the DEAD box helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="JAMZMK010011329">
        <property type="protein sequence ID" value="KAI7727730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BQM7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17939">
        <property type="entry name" value="DEADc_EIF4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000031">
        <property type="entry name" value="Eukaryotic initiation factor 4A-III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 12" id="KW-0347">Helicase</keyword>
    <keyword evidence="4 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="15">
        <location>
            <begin position="40"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="13">
        <location>
            <begin position="89"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="14">
        <location>
            <begin position="231"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="11">
        <location>
            <begin position="40"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAI7727730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="400" mass="45485" checksum="596E5BEC161058BC" modified="2024-05-29" version="1">MAALTQEGSQFDTRQFDTKMDELLSADGQDFLTSYDEVYDSFDSMGLQENLLRGIYAYGFEKPSAIQQRGIVPFTKGLDQLDYTVVECQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVRDDQRILSAGVHVVVGTPGRVFDMLRRQSLPSDNIKMFVLDEADEMLSRGFKDQIYDIFQLLPPKVQVGVFSATMPPEALELTRKFMNKPVRILVKRDELTLEGIKQFYVDAGKEEWKLEILCDLYETLAITQSVIFVNTRCKVDWLTDKMRSRDHTVSATHGDMDQNTRDIIMREFRSGSSRMLITTDLLARGIDVQQVSLVINYDLPTQPENYLHRIGRSGRFGRKGVAINFVTVDDTRMLADVQKFYNVLVEQLPSNLAVRKLHLYRKAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BR21</accession>
    <name>A0AAD5BR21_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">1-phosphatidylinositol-3-phosphate 5-kinase</fullName>
            <ecNumber evidence="1">2.7.1.150</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Phosphatidylinositol 3-phosphate 5-kinase type III</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_007472</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="6">Component of the PI(3,5)P2 regulatory complex at least composed of ATG18, SAC/FIG4, FAB1 and VAC14.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.150" evidence="1"/>
    <dbReference type="EMBL" id="JAMZMK010011290">
        <property type="protein sequence ID" value="KAI7727927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BR21"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000285">
        <property type="term" value="F:1-phosphatidylinositol-3-phosphate 5-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03334">
        <property type="entry name" value="Fab1_TCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.810.10:FF:000001">
        <property type="entry name" value="1-phosphatidylinositol 3-phosphate 5-kinase FAB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000007">
        <property type="entry name" value="1-phosphatidylinositol 3-phosphate 5-kinase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.810.10">
        <property type="entry name" value="2-Layer Sandwich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027483">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002498">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_core"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45748">
        <property type="entry name" value="1-PHOSPHATIDYLINOSITOL 3-PHOSPHATE 5-KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45748:SF4">
        <property type="entry name" value="1-PHOSPHATIDYLINOSITOL-3-PHOSPHATE 5-KINASE FAB1D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01504">
        <property type="entry name" value="PIP5K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00330">
        <property type="entry name" value="PIPKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51455">
        <property type="entry name" value="PIPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PIPK" evidence="10">
        <location>
            <begin position="577"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="626"/>
            <end position="674"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00077223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7727927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="944" mass="106111" checksum="28D3DD0170BDDF09" modified="2024-05-29" version="1">MTYDSLISPPSSSISPAVSLAKTDSTISFYNEYLVADPSNERSLREEARVDGFSLPQPYLGYGGIDSHIWFPPEPEDKEDDIEGSVANCDDDDDDDDVYGDGMKWGKPSHLSRFGQEGSGSFRFREETRKALNEVMNGKFKALVEHLLKSMGISCSGNDGDNWVDIVTALSWEAASFLKPDAFEEKTMDPDGYVKVKCIATGTRSQSEVFKGLVFKKHAAHKHMPTKCKKPKLLLIKGALSVSSDGLSSFESMKQESDRVNHLIEMIEKLNPNVVLVEKTVSRDIQESIYSKGMTLVLEMKQHRLERVARCTTSPILSSDNLNDQKLRQCESFYFEKIVEEHADVSESGKKPKKTLMFLEGCPSRMGFTILLKGCHNDELKKVKCVVQFAVIMAYHLILESSFILNQRAMFSTTAPDGMTIFSNNDEETISSVSSQPLYTNLDDTHVPIVNESSPETSPINTVDIPISSHLDENGSDNLGLEGTSLSSYDAYDPIVLSGLSSLSASLQKVVGIPLFDTVGYNDVNTHNHSEANGKDLSSTETVDKADTEVKVGVEETNFENSEECIQRKDDIRAVLDSESILVLMSRRNVARGITSTYSVSLPPWKVEFSNSTEGDYLNEEVEDVLQEGNSLFTEVENSLNKMENESKKLSDVKEMLKQEQDQFQVDMKNLTTNSSSDVYVNKWLQKPLYLNHKRWELFLRSYVWDERLYSILSSDLGTIGPQITDESGTEQSSFEEKDNIDHEMEIEAVSDDHSYLKMNTAEETNSIPSEVITAEIDIESSNSVQENGVQENVDGGGDVLLDQNFVNDMNVSPLYVNTKAKRRLQRAVWNDTAFLYSINVMDYSLLVGVDVEQKELVCGIIDYVRQYTWDKQLENWVKSFVVPKNQLPTIISPKEYKKRFRKFIDTHLFAVPDDWCSQRSSNRCSLCGVGSHSKSQHHEEMSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BRE4</accession>
    <name>A0AAD5BRE4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Senescence domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_029983</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011272">
        <property type="protein sequence ID" value="KAI7728107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BRE4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009686">
        <property type="entry name" value="Senescence/spartin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045036">
        <property type="entry name" value="Spartin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21068:SF50">
        <property type="entry name" value="PROTEIN EARLY-RESPONSIVE TO DEHYDRATION 7, CHLOROPLASTIC-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21068">
        <property type="entry name" value="SPARTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06911">
        <property type="entry name" value="Senescence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Senescence" evidence="2">
        <location>
            <begin position="221"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="20"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7728107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="416" mass="44386" checksum="0AB9563E5689C47A" modified="2024-05-29" version="1">MASQPPKSLYPQVDQSNPDSLSSFTSSQSTMYPSLQPNDLAQKLFPDPDQQQQIPIESSEEVVIKIPNSIVHLIDKQQSIELASGVFEIIRLRQSGNVVAVLARISDDIQWPLAKDEAAVKLDGSHYFFTLRVPSDSTNNDVVPEDDAINYGLTIPGNDVVLGEELDKVLDEYSAFTMKEVESGGAVVVAEGGGTAEARAAAYWTTLAPNVEDYSGSVARMIAVGSGQLIKGILWCGDVTVDRLKWGNEVLKKRMKAGGKSEVSPEALRRMKRVKKLTKMSESVATGLLSGVVKVSGFFTGAIVNSVPGKKFFNLLPGEIILASLDGFNKVCDAVEVAGRNVMSTTSTVTTDLVTHKHGEDAAKVTSTGMDAAGHAIGTAWAVFKIRKALNPKSVVKPTTLVKAAASKSSSSKSAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BS25</accession>
    <name>A0AAD5BS25_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">MPN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_009507</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M67A family. BRCC36 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011386">
        <property type="protein sequence ID" value="KAI7727401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BS25"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070552">
        <property type="term" value="C:BRISC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070536">
        <property type="term" value="P:protein K63-linked deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08068">
        <property type="entry name" value="MPN_BRCC36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000042">
        <property type="entry name" value="Mov34/MPN/PAD-1 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040749">
        <property type="entry name" value="BRCC36_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000555">
        <property type="entry name" value="JAMM/MPN+_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050242">
        <property type="entry name" value="JAMM_MPN+_peptidase_M67A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033860">
        <property type="entry name" value="MPN_BRCC36"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10410">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18110">
        <property type="entry name" value="BRCC36_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01398">
        <property type="entry name" value="JAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00232">
        <property type="entry name" value="JAB_MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102712">
        <property type="entry name" value="JAB1/MPN domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="MPN" evidence="10">
        <location>
            <begin position="6"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="381"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="353"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="414"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7727401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="431" mass="47128" checksum="E6BDC645817C32D5" modified="2024-05-29" version="1">MSLTSVKMSEDVWLTCLTHALSTETEEIMGLLLGDIQHSKNGVTALIWGALPQPRSDRQKDRVETNPEQLTAASVEAEKMTAETGRTTRVIGWYHSHPHITVLPSHVDVRTQAMYQLLDSGFIGLIFSCFNEDQYKVGRIQVTAFQSLDGKQSNMLRTVPASPISKSSIIDLESSLSTDNSLTTSGSTRVEGLELDTGDSRAAVGISKGGGKSSALKGFFANADSNYSGRIGSNFPASNIETSIVDIDPMDMSDSMQEAMHRSNLEMSGAEYVRKEVPLHVLPTLSLLNLESPLKSFTDLQSVLYEEEKTAYHQAILQNMRDGKVHPLSFIHHTSTYQASICKLMEYCLSPAINALQDRIKENEIRLSMLATEAKILEKESTRGVDLSSSSNSPRKVSSHGGGSPKDLYSPFESNNVRSVSGSSSRSRKGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BS36</accession>
    <name>A0AAD5BS36_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_001070</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the iron/ascorbate-dependent oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011193">
        <property type="protein sequence ID" value="KAI7728552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BS36"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.330:FF:000018">
        <property type="entry name" value="2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050295">
        <property type="entry name" value="Plant_2OG-oxidoreductases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47991">
        <property type="entry name" value="OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0408">Iron</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="5">
        <location>
            <begin position="205"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7728552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="354" mass="39928" checksum="B78FED035EC3CE37" modified="2024-05-29" version="1">MASTDSLPAKTAYQMAIDGDQPPSKYILSNTNNKFGSLETSPPFAPVPIIDISCFVSSSKQDDDQQAELAKLRSALSTWGCFQAVNHGLPESYLDSVRQVIKQFLELPLEEKRKYFREAGSAEGYGNDVTVSESQVQDWCDRLSLRVLPKDQRNLEFWPENPSNFRETIDDFTMKIKSISGVIFKAMAKSLDLEENCFSKQFTEEHDVMKGRFILYPPCPTPDQVFGLKAHSDRSGITLLLQDPGTEGLQVCNDGKWYMVPVLRDALFINLGDQMQIMSNGIFKSPLHRVVTNKEKGRISVAMFTEPDPNKEIGPVDVLVDEKRPRVYRTVKNYAVFNYECFQKGIVALDAVKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BSM7</accession>
    <name>A0AAD5BSM7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_001097</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011193">
        <property type="protein sequence ID" value="KAI7728579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BSM7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26312:SF214">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT (TPR)-LIKE SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26312">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7728579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="231" mass="25291" checksum="F7073C017F354BAA" modified="2024-05-29" version="1">MNSPKKLIRASSETDLVINSLYPVIKTTASLIASIAVEEDTEGEESDHEFFMFSTSGLYEVEVDGTGGGGGEGKSLGGHGCGNGHEDDNNNNNNSTDLYYQNMIEANPGNAMLLNNYAKYLKEVRGDFVKAEEYCSRAILANPNDGSVLSMYADLIWETQKDAPRVQNYFDQAVQASPDDCYVMASYARFLWDADDEEEEEVVNQDNCDMNVLTPSFLTGTSQLPPIATAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BTL2</accession>
    <name>A0AAD5BTL2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Inhibitor of growth protein N-terminal histone-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_004719</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011036">
        <property type="protein sequence ID" value="KAI7729232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BTL2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17015">
        <property type="entry name" value="ING_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028651">
        <property type="entry name" value="ING_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024610">
        <property type="entry name" value="ING_N_histone-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10333">
        <property type="entry name" value="INHIBITOR OF GROWTH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10333:SF42">
        <property type="entry name" value="INHIBITOR OF GROWTH PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12998">
        <property type="entry name" value="ING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01408">
        <property type="entry name" value="ING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Inhibitor of growth protein N-terminal histone-binding" evidence="5">
        <location>
            <begin position="9"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7729232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="224" mass="25882" checksum="283EF62F92B3731B" modified="2024-05-29" version="1" fragment="single">MAIARTGVFVDDYLEYASTLPAELQRLLNTIRELDDRSQSMINQTRQQTKSCLDMASQNSYKGIHEDDDMAFEKMKKEIEANQDNALSLCTEKVLLARQAYDLIDSHVKRLDEDLHNFAEDLKQEGKLPADEPAILPPLPLVPKNEKRKLLYSTPQSKKLDYRDREWDHRDRDFELMPPPGGFKRDYATPLEMDQPIDPNEPTYCVCHQVSYGDMIACDNENID</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BVA0</accession>
    <name>A0AAD5BVA0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Piwi domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_004506</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010809">
        <property type="protein sequence ID" value="KAI7730292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BVA0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003165">
        <property type="entry name" value="Piwi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02171">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Piwi" evidence="1">
        <location>
            <begin position="8"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7730292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="37" mass="4065" checksum="69FD6E76812C210C" modified="2024-05-29" version="1">MLTGVEIILSGTVVDSKICHPIEFDFYLCSHAGIQVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BVK8</accession>
    <name>A0AAD5BVK8_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_023097</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010821">
        <property type="protein sequence ID" value="KAI7730260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BVK8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7730260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="41" mass="4918" checksum="90720E2280A958FE" modified="2024-05-29" version="1">MEILTLLEIKYDLIRSALKWHNVIFNLHWAVCRCVRYAAVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BWZ1</accession>
    <name>A0AAD5BWZ1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PI-PLC X domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_032492</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010588">
        <property type="protein sequence ID" value="KAI7730935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BWZ1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08588">
        <property type="entry name" value="PI-PLCc_At5g67130_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.190:FF:000048">
        <property type="entry name" value="PI-PLC X domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051057">
        <property type="entry name" value="PI-PLC_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13593">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13593:SF140">
        <property type="entry name" value="PLC-LIKE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26178">
        <property type="entry name" value="PI-PLC_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50007">
        <property type="entry name" value="PIPLC_X_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041970594" description="PI-PLC X domain-containing protein" evidence="1">
        <location>
            <begin position="29"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7730935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="422" mass="46326" checksum="9D93424143D13169" modified="2024-05-29" version="1">MKKKKNISSFLLLLPIIILFTFLLTTSACSNGDCQILESCSAATDCAPGLYCGNCPELGKTQPFCTRGQAIIPTAIVNGLPFNKYTWLVTHNAFSIVDAPLLTGPQRITFYNQEDSVTNQLRNGVRGLMLDMYDFENDIWLCHSFRGQCYNITAFQPAINMLREVEAFLSSNPTEIVTIIIEDYVHTPKGLNKLFTDAGLDKYWFPVSQMPKKGEDWPTVTSMAEDNHRLLVFTSDSSKEATEGIAYQWRYMVENAPGDPGVQQGSCPNRKESKPLTSKTASLFLQNYFPTMPVQAEACKENSTPLMEMVGTCYKAAGNTMPNFLAVNFYMRSDGGGVFDALDRMNGQTLCGCASVASCQAGAPFGTCRNNAVTNNSTSPRGPSGSFSGSVQMTSSASRIDFTSLLAVYGFYFLMNMVGILL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BX65</accession>
    <name>A0AAD5BX65_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Defective in cullin neddylation protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_011499</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Neddylation of cullins play an essential role in the regulation of SCF-type complexes activity.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010453">
        <property type="protein sequence ID" value="KAI7731388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BX65"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097602">
        <property type="term" value="F:cullin family protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031624">
        <property type="term" value="F:ubiquitin conjugating enzyme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032182">
        <property type="term" value="F:ubiquitin-like protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045116">
        <property type="term" value="P:protein neddylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000162">
        <property type="entry name" value="Defective in cullin neddylation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.200:FF:000005">
        <property type="entry name" value="Defective in cullin neddylation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.200">
        <property type="entry name" value="Cullin, PONY binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014764">
        <property type="entry name" value="DCN-prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042460">
        <property type="entry name" value="DCN1-like_PONY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005176">
        <property type="entry name" value="PONY_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12281:SF12">
        <property type="entry name" value="DEFECTIVE IN CULLIN NEDDYLATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12281">
        <property type="entry name" value="RP42 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03556">
        <property type="entry name" value="Cullin_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51229">
        <property type="entry name" value="DCUN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DCUN1" evidence="2">
        <location>
            <begin position="35"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU410713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7731388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="230" mass="26925" checksum="EC8AE1472EA37851" modified="2024-05-29" version="1">MRRASSKKASSNSTTSTNSSAMDLFRSATGKAATKELERIDQLFYSYSNESSGMIDPEGIEYLCSDLEVEHTDVRILMLAWKMQAEKQGYFTLEEWRKGLKALHADTIVKLKKALPELENEVRRPSNYVEFYSYAFRYCLTEEKQKSIDIESICELLELVLGSQFPQQVDFFVQYLKTQVDYKVINMDQWMGFYRFCNEISFPDFNNYDEELAWPLILDNFVDWVKSKQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BYJ7</accession>
    <name>A0AAD5BYJ7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_017008</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010361">
        <property type="protein sequence ID" value="KAI7731897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BYJ7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42647:SF22">
        <property type="entry name" value="BOI-RELATED E3 UBIQUITIN-PROTEIN LIGASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42647">
        <property type="entry name" value="SBP (S-RIBONUCLEASE BINDING PROTEIN) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="168"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7731897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="268" mass="31449" checksum="BF13797906FEDC05" modified="2024-05-29" version="1" fragment="single">SLCFLLITRGHPTPNQKHHHITKENESLFLYIQTHQLLIFRPTDGGYRMAIQARLYSENLVFPFINDDGCGDANPSQDLIENACCLNDLYLYNLQQQQQYQPQPAHVNHDSCFNNEFNKPMSLQYYSSSVAANIQKQNQEIEGMISLQHEKMRLALQAHKKQQILTILKNYESKSKLLLRQKDEEIKRATKRRIELEEFLTKTDIERQKWQIAAKETEAMVINLNNTIEQLVSDQNMKKHAEDEGSCCYDNNENMKTKEMMNIIHKFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BYQ1</accession>
    <name>A0AAD5BYQ1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SKP1 component dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_008969</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SKP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010350">
        <property type="protein sequence ID" value="KAI7731957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BYQ1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009867">
        <property type="term" value="P:jasmonic acid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016897">
        <property type="entry name" value="SKP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001232">
        <property type="entry name" value="SKP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036296">
        <property type="entry name" value="SKP1-like_dim_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016072">
        <property type="entry name" value="Skp1_comp_dimer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11165">
        <property type="entry name" value="SKP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01466">
        <property type="entry name" value="Skp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00512">
        <property type="entry name" value="Skp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81382">
        <property type="entry name" value="Skp1 dimerisation domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="SKP1 component dimerisation" evidence="5">
        <location>
            <begin position="112"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="202"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="214"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="231"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7731957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="351" mass="39687" checksum="579A94DBFC3BF086" modified="2024-05-29" version="1">MSIVKPEMKSFVWLQTGDGLIQQVEEEVAMFCPMICREVFQKSMGSSKNHAIKLPERVTPAVLGLILDYCRFHQAAGRSNKERKIFDEKFIRLDASKLCDLVSAADSLQLRPLVDLTSCAIARMISGKTAEEIRETFHIADDLTEEEKLEPIKFKTDDSRVRLLNTLYARKRKQLKAREQSKNVEVEEERVEDRSVDDLLSFINSGDEDSKGIKPAKNKKRNRRKKHLRKDRSPNIENKYHYKETDSLSSSHNSEVEDMPNQSISLKLLGYASAESDFDDGLDPALKAQLDRHGREVGPISSHNAVWSTFVGMLLLGHYTATIVNGGPLLGLGKSFDLLKTKENENCSCKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BZU5</accession>
    <name>A0AAD5BZU5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_005248</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010206">
        <property type="protein sequence ID" value="KAI7732532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BZU5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044986">
        <property type="entry name" value="KIF15/KIN-12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37739">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIN-12D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37739:SF8">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIN-12D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0493">Microtubule</keyword>
    <keyword evidence="5" id="KW-0505">Motor protein</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7732532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="162" mass="18411" checksum="DBFD93CE3C89C93B" modified="2024-05-29" version="1">MMLPRMDDDDNHDDDEEMEIVEEAEQLVPVDFEPLDDNISKQIVIPKAVEKVLSGAIRRKMALEEFCTRQNAEIMQLNRLVQQYKHERECNLIIGQLQEDKIARLENLMEGVPLAEEFVDEDLTSLANQHVPTIVLASRLPDLSGMVLHSPHVASGQTNLLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C2X2</accession>
    <name>A0AAD5C2X2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_006008</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009743">
        <property type="protein sequence ID" value="KAI7734371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C2X2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7734371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="77" mass="8816" checksum="B7120CE561B9E911" modified="2024-05-29" version="1" fragment="single">MFMLTTERLLTIKFAILNTVTSTFVLRIDRLEPPIPPITMFFSIILVSLQISCMSLCIRYAMLQQVVVVSTNFPSFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C545</accession>
    <name>A0AAD5C545_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_032052</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009475">
        <property type="protein sequence ID" value="KAI7735541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C545"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052059">
        <property type="entry name" value="CR_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47973">
        <property type="entry name" value="CYSTEINE-RICH RECEPTOR-LIKE PROTEIN KINASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="1"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7735541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="161" mass="18065" checksum="EECE3B09352566CF" modified="2024-05-29" version="1">MAPEYLAHGQLTEKADVYSFGVLILEVVTGMENNKSKTTEYTESLPSTAWKHFKQDTVLEIFDPNLMMHSYRNNDFQKDAIKVVHVGLLCTQEAPSLRPSMSAALKMLAKDDEPLPAPSSPPFMDEKTMELNNIRQRLGNHCDGDDCSSVASVSHSQFYPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C636</accession>
    <name>A0AAD5C636_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Plectin/eS10 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_011858</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic ribosomal protein eS10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009467">
        <property type="protein sequence ID" value="KAI7735569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C636"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005326">
        <property type="entry name" value="Plectin_eS10_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037447">
        <property type="entry name" value="Ribosomal_eS10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12146">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12146:SF0">
        <property type="entry name" value="RIBOSOMAL PROTEIN S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03501">
        <property type="entry name" value="S10_plectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Plectin/eS10 N-terminal" evidence="7">
        <location>
            <begin position="2"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="30"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="47"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="69"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="102"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7735569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="120" mass="12989" checksum="8BA4CBF0C189AC1F" modified="2024-05-29" version="1">MHYYWYLTNDGIEFLRTYLNLPSDIVPATLKKSAKPLGRPTGGPPGDRPRGPPRFEGDRPRFGDRDGYRGGPRGPPGEFGGEKGGAPADYQPAFNRERLATSRRLGSGSRPSFGRGGGGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C665</accession>
    <name>A0AAD5C665_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="2 5">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_022325</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="2 5"/>
    <dbReference type="EMBL" id="JAMZMK010009459">
        <property type="protein sequence ID" value="KAI7735614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C665"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43811">
        <property type="entry name" value="FKBP-TYPE PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FKPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43811:SF32">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FKBP16-4, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase FKBP-type" evidence="7">
        <location>
            <begin position="90"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="50"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7735614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="186" mass="20553" checksum="62493541A3B2FFBE" modified="2024-05-29" version="1" fragment="single">MDEVQQIGSVGQDGETEIMVNGSSVTHGEPPQNGQDAEEEIVMEGSSFVQGGPPQDDNGPPKVDTKVEAIHEKVTKQVIKEGHGEKPSKYSTCFLHYRAWTESTQHKFEDTWQELQLTEVVLGKEKKEMAGLALGLYSMKAGERALLHVGWELGYGEEGSFSFPNVPPKADIIYEVELVGFDETKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C689</accession>
    <name>A0AAD5C689_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Myosin-6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_008792</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="9">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009631">
        <property type="protein sequence ID" value="KAI7734854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C689"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030048">
        <property type="term" value="P:actin filament-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd15475">
        <property type="entry name" value="MyosinXI_CBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.530:FF:000002">
        <property type="entry name" value="Class V myosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.720:FF:000011">
        <property type="entry name" value="Myosin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.190:FF:000018">
        <property type="entry name" value="Myosin XI D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.190:FF:000001">
        <property type="entry name" value="unconventional myosin-Va"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.190">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1590">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002710">
        <property type="entry name" value="Dilute_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037975">
        <property type="entry name" value="MyosinXI_CBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140">
        <property type="entry name" value="MYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140:SF836">
        <property type="entry name" value="MYOSIN-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01843">
        <property type="entry name" value="DIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM01132">
        <property type="entry name" value="DIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51126">
        <property type="entry name" value="DILUTE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="5 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0505">Motor protein</keyword>
    <keyword evidence="6 9" id="KW-0518">Myosin</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Myosin motor" evidence="12">
        <location>
            <begin position="1"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="domain" description="Dilute" evidence="11">
        <location>
            <begin position="843"/>
            <end position="1084"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="9">
        <location>
            <begin position="308"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="585"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="666"/>
            <end position="742"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7734854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1138" mass="129729" checksum="4BE9E071A201AE39" modified="2024-05-29" version="1">MDVVGINSEEQDAIFRVVAAILHLGNIEFAKGKEMDSSTPKDEKSWFHLKTAAELFMCDVKALEDSLCKRVIVTRDETITKWLDPESAAVSRDALAKVVYSRLFDWLVDRINSSIGQDPDSKYIIGVLDIYGFESFKTNSFEQFCINLTNEKLQQHFNQHVFKMEQEEYTKEEINWSYIEFIDNQDILDLIEKKPGGIIALLDEACMFPRSTHETFAQKLYQTFKNHKRFAKPKLARSDFTICHYAGDVTYQTELFLDKNKDYVIAEHQALLSASKCSFVACLFTTSSDESSKSSKFSSIGTRFKQQLQQLLETLSSTEPHYIRCVKPNNLLKPAIFENHNVLQQLRCGGVLEAIRISCAGYPTRKPFDEFLDRFGILAPEVLDGSDEIRACKKLLEKAGLEGYQIGKTKVFLRAGQMAELDSRRTEVLGRSASIIQRKVRSFIAQKSYISLKRSALQIQSVCRGQLARQIYESMRREASSIRIQRNLRMHLSRKAYKELQFSAVSIQTGLRGMAARNELRFRRQTKAAILIQSHCRKFLARLHFIKAKKGAVSLQCAWRGKVARKELRKLKLDARQTGALQAAKNKLEKQVEDLTWRLQLEKRMRSDLEEAKTQENAKLQAALQDMQLQFKETKELLMKEREIAKKVVETVPVIQEVPIVDHELTNKLASENEKLKALVSSLEKKIDDAEKKYEESNKLSEERLKQALDAETKIIQLKTAMQSLQEKVSDMASENQILRQKGLSTTASRVAEYPQTPDAKSMANGHFGHEEPQTPARNLTTEFDSKAKRPPIDRQHENVDALIECVMKDIGFSQGKPVAAFTIYKCLIHWKSFEAEKTSVFDRLIQMIGAAIEDQDNNEHMAYWLSNASTLLFLLQRSIKSDGANSVRKPPPPTSLFGRMTMGFRSSPSTVNLTAAAAALEVVRQVEAKYPALLFKQQLTAYVEKMYGIIRDNLKKELGSFLTLCIQASLLHFCLLLRRECCTFSNGEYVKAGLAELELWCCQAKEEYAGSAWDELKHIRQAVGFLVIHQKYRISYDEIIHDLCPILSVQQLYRICTLYWDDNYNTRSVSPDVISSMRILMTEDSNNAASSSFLLDDNSSIPFSVDDLSTSLQVKEFSDVKPAVELAENPAFQFLHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-06-18" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C6B1</accession>
    <name>A0AAD5C6B1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_017888</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">May glycosylate diterpenes or flavonols in leaves.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009476">
        <property type="protein sequence ID" value="KAI7735530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035251">
        <property type="term" value="F:UDP-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000056">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000080">
        <property type="entry name" value="Glycosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050481">
        <property type="entry name" value="UDP-glycosyltransf_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48048">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48048:SF45">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00053747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7735530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="1381" mass="152738" checksum="1A63570351A7F0E0" modified="2024-05-29" version="1">MANSVAELVFIPAPGLGHIMSTIEIAKLFVNRDQRFSITILVIKPPSSSSGSAVTAYFESLGKKSINRVSFVELPQEDAPPMHDLISFVTSFNEYVKSHCKYVRKVVAELMSQPGSGRLAGFVVDMFCTSMVDVANEFNAPTYVFFTSGAAFLGFEFFMQALNDEHKQDLVQLTNSDTEVLVPSFVKPVPTKVFWSVLRTKEGMEFAMSGARKLREVKAIMVNTFLELETHAFESLSADISVPTVYPVGPILNLEGSSGKPLDDDVFKWLDNQPPSSVVLLCFGSMGSFDEVQVKEIAHALELSGHRFVWSLRRPPAEETTKIPSDYEDPGAVLPEGFLERTNGVGKVIGWAPQVALLAHSAVGGFVSHCGWNSLLESVWFGVPVATWPVYSEQQMNAFQMVVELGLSVEIKWDYIRDLFDPSAKTDIICAKEIESGIRHVMEDQEVRRKVKEMSEKSRAAVAKGGSSYAAVGNLIQDFIIAHLVFIPVPGLGHIMSTIELAKLFMNRDQRLSVIWITSFIELPQDETPPTRDSKSPLTSFNEHINSHCKYVRNIVGDMIGQPGLGRVVGFVIDMFCTCMIDVANEFNVPTYVFCTSSAAFLGFELYVQTMFDVQNQDVIQLSHSDTDVSISTFSKPVPTKVFPSVVQTREALDVFLSYARRLREVKAIIVNSFIELESYAIKSLSYDNTVPPVYPVGPILNLESGSSVGKQLDNDVLKWLDNQPPSSVVFLCFGSMGSFEEVQVKEIACALEKSGHPFVWSLRQPSAGKLSTVTGDYENPKVVLPKGFLERTAGVGKVIGWAPQVAVLAHMATWPIYAEQQINAFEMVVEIGLAVEIKMDYKRDMFIPIAETEMVVVTSEEIESGIRRLMEDDLVRTKVKIMSEKCRSTVVEDGSSYESFGSLVFIPAPGHGHIMSTIEIAKLFVNRDQRLSVTVLVIKPPSSGSGSTTTYIESLGKRSADRISFIELPQQEIAPPSDSKGPLTSFSEFIKSHCKYVRNIVADMISQPGSGQVAGFVIDMFCTCMIDVANEFNVPTYVFYTSNAAFLGFKLFIQTLNDVQNQDVVELSNSDTEIPVPTFVTPVPTKVFPSVVQTRRTLDFVLSSARGLREVKAIIVNSFMELETHAIESLSADNTIPPVYPVGPILNLEDGSGAGKPLDDDIIKWLDNQPPSSVVFLCFGSMGSFDEVQVKEIARALEQSGHRFVWSLRQPPLDKPPRVTRDYEDFRVVLPEGFLERTSGIGKVIGWAPQVAVLGHSAVRGFVSHCGWNSLLESLWFGVPVAAWPMYAEQQLNAFEMVVEMGLAIEIKLDYKKDSFVHVGETVVVTAKEIENGIRRVMEDDLVRTKVKKISERSRSTVKEGGSSYASFGSLIQDIITNIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C6W2</accession>
    <name>A0AAD5C6W2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_022401</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009398">
        <property type="protein sequence ID" value="KAI7735989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C6W2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03213">
        <property type="entry name" value="ABCG_EPDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000367">
        <property type="entry name" value="ABC transporter G family member 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050352">
        <property type="entry name" value="ABCG_transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041:SF142">
        <property type="entry name" value="P-LOOP CONTAINING NUCLEOSIDE TRIPHOSPHATE HYDROLASES SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19055">
        <property type="entry name" value="ABC2_membrane_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042152032" description="ABC transporter domain-containing protein" evidence="11">
        <location>
            <begin position="26"/>
            <end position="1092"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="289"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="863"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="892"/>
            <end position="912"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="968"/>
            <end position="989"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1068"/>
            <end position="1089"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="487"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="352"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7735989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="1092" mass="121810" checksum="6606940FF3E85C0F" modified="2024-05-29" version="1">MTRWKLFAAVAIAVVVLYSPEFVLSQVVGDEFAGQINDTAFLPFITKRVYSSLSNITSTILNSDVGNRASFCVKDREAEWDRAFNFSNNLDFLTACIQRTNGDVTRRVCTSAELKFYFGAFFSGDSTYLKPNRNCNITSRVSGCEPGWACATSPDENIDMEDSKVIPARTESCQPCCEGFFCPSGLTCMIPCPLGAHCPTATLDKASGICKPYSYQLPPGMPNHTCGGANIWADIRTSEELFCSSGSYCPTSTQQLICSKGNYCQRGSTSEKSCFKLTSCDPGSSSQNIHAYGAMLIAAIFTIMLIIYNCSDQIITTRERRHAKTREAAARRAKEKVKAHERWKMAREAAKKHAAQISRTFSRKKKPAPQTEEMSILHQSSEDVEFDEPVTSSSEENRLELRSQTFKKKDEDLYIYDHILEMESKVTNSKKKMPKEKQIHTHSQIFNYAYGQIEKEKAMQENQNLTFSGVISMAVNTETRKRPKIEISFRDLTLTLKGKDKHLLRCVTGKIMPSRITAVMGPSGAGKTTFLSALAGKAHGCKITGSILINGKPDSIHSYKKIIGFVPQDDIVHGNLTVEENLWFSAKCRLPASMLKQDRVLVVERVIESLGLQAVRSSLVGTVEKRGISGGQRKRVNVGLEMVMEPSLLILDEPTSGLDSSSSQLLLRALRRETLEGVNVSMVVHQPSYSLFQMFDDLVLLAKGGLTVYHGPVRKVEEYFSGLGINVPDRVNPPDYFIDVLEGMIKLNTSSGVSYEQLPVRWMLHKGYPVPPDMRKNAAMYPEGQVSGNEVNDNEGAAEEYSLAGEIWQDVKSNVEVRRDIIRHNFLRTKDLSNRTTPGVLLQYRYFLGRIAKQRLRESKLQLVDYLILLLAGACLGAIIKSNEETFGAPGYTYSIIAVSLLCKVAALRTFSLDKLQYWRERASGISSLAHFLAKDTIDHFNTAIKPAVYLSMFYFFSYPRSSFAENYTVLLSLVYCVTGIAYMLAIFLDPGPSQLFAVLLPVVLTLVSTQTGDSEFLKNVSKLCYPKWALEAFVIANANRYSGVWLITRCGALLKFGYNVHDWGLCIFVLFMIGVASRISAFFGMLVVHKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C721</accession>
    <name>A0AAD5C721_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">M8C21_000485</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="22">AA19_3_7</strain>
            <tissue evidence="22">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010009257">
        <property type="protein sequence ID" value="KAI7736501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C721"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000217">
        <property type="entry name" value="probable LRR receptor-like serine/threonine-protein kinase At1g53430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000044">
        <property type="entry name" value="Putative LRR receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.430">
        <property type="entry name" value="Galactose-binding lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051824">
        <property type="entry name" value="LRR_Rcpt-Like_S/T_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021720">
        <property type="entry name" value="Malectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48006">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN DDB_G0281931-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48006:SF66">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11721">
        <property type="entry name" value="Malectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 18 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="13 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 18 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-0675">Receptor</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 19" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="84"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="21">
        <location>
            <begin position="142"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="22">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="KAI7736501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="479" mass="53008" checksum="55D3A7D63CFA9237" modified="2024-05-29" version="1" fragment="single">GVSTIKNFDIKNEAGGVDKAVIKIFNNILVTNRTLEIRLQYAGKGTTAVPMRGLYGPLISAISIESEFMPLELKQVTHGKKRTFLVIGVVAAVFLFILIVVAIAWKKGYIGNQISRERDLRGLDLNTGVFTYKQIRAATDNFAESNKLGEGGFGSVYKGTLLDGTLIAVKQLSSKSKQGNREFVNEIGMMAGIQHPNVVRLHGCCAEHNQLLLVYEYMENNSLAHALFDPDKSKMEIDWPTRQRICVGIATGLAFLHEETALKMVHRDIKAANILLDTNLTPKISDFGLAKLNEKENTHISTRVAGTIGYMAPEYALWGHLTYKADVFSFGVLALEIIAGKNNMNYRPNEDYVSLLDLAIVLEEKGSLMDLVDPRLGSDFNKIEAVRMMEVALLCTNQSPALRPTMPDVVNLLEGLPAKIKESDMNLATSEDDFRLQSVIGKVEDSQLQNHNAVQQSSTSIYDLRPNSQISEESQINCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C7U6</accession>
    <name>A0AAD5C7U6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_015823</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009187">
        <property type="protein sequence ID" value="KAI7736817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C7U6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7736817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="124" mass="13517" checksum="A98577ADF13CE715" modified="2024-05-29" version="1" fragment="single">TNMLQYADQQVQVHPHLHPTMDFNNPLWSGVVDAPDWVDHSSYAPSLLSSSVAPPQASVSTLSHVMYPPSESGSVTLAASPLDQGPSYGGSENDYEVGWGGVTDMDPHYHESSDPQTLPFYWQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C944</accession>
    <name>A0AAD5C944_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Branched-chain-amino-acid aminotransferase</fullName>
            <ecNumber evidence="9">2.6.1.42</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_022784</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-isoleucine + 2-oxoglutarate = (S)-3-methyl-2-oxopentanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24801"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:35146"/>
            <dbReference type="ChEBI" id="CHEBI:58045"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:18321"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:17865"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-valine + 2-oxoglutarate = 3-methyl-2-oxobutanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24813"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57762"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.42" evidence="9"/>
    <dbReference type="EMBL" id="JAMZMK010008985">
        <property type="protein sequence ID" value="KAI7737613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C944"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004084">
        <property type="term" value="F:branched-chain-amino-acid transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009082">
        <property type="term" value="P:branched-chain amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01557">
        <property type="entry name" value="BCAT_beta_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.10.10:FF:000003">
        <property type="entry name" value="Branched-chain-amino-acid aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.10:FF:000003">
        <property type="entry name" value="Branched-chain-amino-acid aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.10.10">
        <property type="entry name" value="D-amino Acid Aminotransferase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001544">
        <property type="entry name" value="Aminotrans_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018300">
        <property type="entry name" value="Aminotrans_IV_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036038">
        <property type="entry name" value="Aminotransferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005786">
        <property type="entry name" value="B_amino_transII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043132">
        <property type="entry name" value="BCAT-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043131">
        <property type="entry name" value="BCAT-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033939">
        <property type="entry name" value="BCAT_family"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01123">
        <property type="entry name" value="ilvE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009897">
        <property type="entry name" value="PRK13357.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42825">
        <property type="entry name" value="AMINO ACID AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42825:SF2">
        <property type="entry name" value="BRANCHED-CHAIN-AMINO-ACID AMINOTRANSFERASE 3, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01063">
        <property type="entry name" value="Aminotran_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006468">
        <property type="entry name" value="BCAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56752">
        <property type="entry name" value="D-aminoacid aminotransferase-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00770">
        <property type="entry name" value="AA_TRANSFER_CLASS_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="3 9" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="9" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="5 8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="6">
        <location>
            <position position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR006468-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7737613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="406" mass="43723" checksum="C9210593ED7E49FD" modified="2024-05-29" version="1">METAAVFSTAAATTTTTVAGRCFLRSPHLSFLPKLRNQLRFGSLNTCNIYANVNARAIEVASSLSVPSETVELDWDNLGFGLVHTDYMYTMKCSLGEAFTKGELVPFGNIEINPSAGVLNYGQGLFEGLKAYRTEDGNILLFRPEENAKRMIAGAERMCMPSPSVDQFLQAVKATVLANERWIPPPGKGSLYIRPLLMGSGSVLGLAPAPEYTFLIYVSPVGNYFKEGLSPINLVVETEMHRATPGGTGGVKTIGNYAAVLKAQGAAKAKGYSDVLYLDSTHKKYLEEVSSCNIFVVKGNTISTPDIKGTILPGITRKSIIDVARDQGFEVFEKPVAVDELLAADEVFCTGTAVVVSPVGSITYLGKRVTYGNTGVGLVSQQLYSGLTRLQMGLADDKMGWIVELK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C9G8</accession>
    <name>A0AAD5C9G8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_027856</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008972">
        <property type="protein sequence ID" value="KAI7737687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C9G8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45763:SF36">
        <property type="entry name" value="AB HYDROLASE-1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45763">
        <property type="entry name" value="HYDROLASE, ALPHA/BETA FOLD FAMILY PROTEIN, EXPRESSED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="183"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7737687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="332" mass="37660" checksum="4D63FB55A172D77B" modified="2024-05-29" version="1">MNGFNGGAPPPPPPYAAGYRTNVHRVSPDDWRFHATEFAKGVAELTVEFGKGCRDVVKQSILRDDSFVVRTFNGPCKSACMRLKFLNQYLPEDRDPVHSWSVISFVFAVVIAVLIVTSESDASIPMIQKLHIYPGNATRILLPDGRHLAYQEQGVSYERARFTLVTSHSFLSSRLAGIPGIKASLLEEFGVRLVTYDLPGFGESDPHPGRNLESSAMDLLHFSYAVHITDKFWMVGYSGGSLHAWAALKYIPDRIAGAFMVAPLLNPYELSMNKVERRRTWEKWTLRRKFMYFLARKFPVLLPYFYRRSFLSGNIDQIDKWLALSLGKRMSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CAL2</accession>
    <name>A0AAD5CAL2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Monocopper oxidase-like protein SKU5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">M8C21_021536</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">AA19_3_7</strain>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <topology evidence="2">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the multicopper oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008818">
        <property type="protein sequence ID" value="KAI7738406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CAL2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13846">
        <property type="entry name" value="CuRO_1_AAO_like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000012">
        <property type="entry name" value="Monocopper oxidase-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000016">
        <property type="entry name" value="Monocopper oxidase-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000023">
        <property type="entry name" value="Monocopper oxidase-like protein SKU5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001117">
        <property type="entry name" value="Cu-oxidase_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034273">
        <property type="entry name" value="CuRO_1_AAO-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF58">
        <property type="entry name" value="SKU5 SIMILAR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00394">
        <property type="entry name" value="Cu-oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0186">Copper</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="544"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="15">
        <location>
            <begin position="11"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="13">
        <location>
            <begin position="136"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="14">
        <location>
            <begin position="370"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAI7738406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="565" mass="63145" checksum="8E8AEA9DA9235E20" modified="2024-05-29" version="1" fragment="single">DPYVYYDWTLSYITASPLGVKQQVIAINGHFPGPILEATTNWNVVVNVKNDLDEPVLLTWNGIQHRKNSWQDGVLGTNCPIPAGWNWTYSFQVKDQIGSFFYFPSTNFQRAAGGYGGIIINNRDVIPVPFGNPDGDITLNIGDWFVKSHKDLRKNIENGVDLGSPDGILFNGLGPYRYDSTIVPDGIEYQVINVEPGKTYRFRIHNVGISTSLNFRIQNHNLLLVETEGSYTVQQNYTNMDIHVGQSYSFLVTMDQTGSNDYYIVASPRFLNSPKAVGVAILHYSNSQGPVSGPLPDIPNESDPSFSMNQARSIRMNVTAGAARPNPQGSFKYGEITVTEVYLLQNRPPELINGKRRTTLNGISFFTPSTPLKLAQQYNVLGIYKLDFPNKPMNRPPVIDTSVINGTYKGFIEIILQNNDTTVQSYHLDGYAFFVVGMDYGSWTENSRSVYNKWDGVARSTTQVFPGAWTAILVYLDNAGLWNLRAQNLDSWYLGQEVYLSVVNPEVSEKTELPVPENTIYCGVLSSLQNDQAQRVRFSGAPPIPMVITRVFIAFIIAFVGDSMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CAR6</accession>
    <name>A0AAD5CAR6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_020177</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008939">
        <property type="protein sequence ID" value="KAI7737843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CAR6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000140">
        <property type="entry name" value="Myb transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051953">
        <property type="entry name" value="Plant_SW-associated_TFs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47997">
        <property type="entry name" value="MYB DOMAIN PROTEIN 55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47997:SF76">
        <property type="entry name" value="OS07G0497500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="8">
        <location>
            <begin position="8"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="7">
        <location>
            <begin position="8"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="8">
        <location>
            <begin position="55"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="7">
        <location>
            <begin position="55"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7737843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="334" mass="37935" checksum="12D09134CA7B6827" modified="2024-05-29" version="1">MGHHCCSKQKVKRGLWSPEEDEKLIKHITNHGHGCWSSVPKLAGYCRLRWINYLRPDLRRGSFTQQEERTIIDVHRILGNRWAQIAKHLPGRTDNEVKNFWNSCIKKKLISQGLDPNTHNLLSPHQIPILNNANISKNIDPIKPVFAIINNSSSQTKDAKKVQPFQPHLSINCNDLVMSHNLYENTLTRESSDLNYEDYPLFLKNDVRHESTSGFSSQPLVESTKMPVLLPLSCSSNPLDLRTVVQSCAKNSCLGVVSEHTRRPEMYRETLQSQMVCEGLLAKEFVNKGMDLTNGDMSLLDGCNIDLEFMEAALMPCGMFYNVGSLDQLAWDCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CBG9</accession>
    <name>A0AAD5CBG9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_021094</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008720">
        <property type="protein sequence ID" value="KAI7738797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CBG9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7738797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="62" mass="7223" checksum="E2554A29C5755DC8" modified="2024-05-29" version="1" fragment="single">MQKEENQASSSYNNECFEIIDPVYLKLHSITFFRGDEAMITESFKKQSKVRMVGTYITIPDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CBS2</accession>
    <name>A0AAD5CBS2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">GPN-loop GTPase 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_031587</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Small GTPase required for proper nuclear import of RNA polymerase II and III (RNAPII and RNAPIII). May act at an RNAP assembly step prior to nuclear import.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Binds to RNA polymerase II (RNAPII).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the GPN-loop GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008811">
        <property type="protein sequence ID" value="KAI7738458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CBS2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17872">
        <property type="entry name" value="GPN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000552">
        <property type="entry name" value="GPN-loop GTPase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004130">
        <property type="entry name" value="Gpn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030228">
        <property type="entry name" value="Gpn3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231:SF7">
        <property type="entry name" value="GPN-LOOP GTPASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231">
        <property type="entry name" value="XPA-BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03029">
        <property type="entry name" value="ATP_bind_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7738458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="268" mass="30606" checksum="90D5E0D0C4E0E53D" modified="2024-05-29" version="1">MGYAQLVIGPAGSGKSTYCSSLYQHCEATRRTIHIVNLDPAAENFDYPVAMDIRELISLDDVMEELGLGPNGGLLYCMEHLEENLDEWLNEELENYMDDDYLVFDCPGQIELFSHVPVLKNFVEHLQRKNFNVCAVYLLDSQFITDVTKFISGCMASLSAMVQLELPHVNILSKMDLVARKRDIEDYLNPEPQTLLAELNQRMGPQFQKLNKALIELVDQYSMVSFVPLDLRKESSIQYVLSQIDNCIQFGEDADVKIKDFDEDEDQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CD20</accession>
    <name>A0AAD5CD20_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent)</fullName>
            <ecNumber evidence="5">5.4.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">M8C21_004435</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">AA19_3_7</strain>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the reversible isomerization of alpha-D-glucose 1-phosphate to alpha-D-glucose 6-phosphate. The mechanism proceeds via the intermediate compound alpha-D-glucose 1,6-bisphosphate. This enzyme participates in both the breakdown and synthesis of glucose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>O-phospho-L-seryl-[protein] + alpha-D-glucose 1-phosphate = alpha-D-glucose 1,6-bisphosphate + L-seryl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:68748"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:58392"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>alpha-D-glucose 1,6-bisphosphate + L-seryl-[protein] = O-phospho-L-seryl-[protein] + alpha-D-glucose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:68752"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:58225"/>
            <dbReference type="ChEBI" id="CHEBI:58392"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>alpha-D-glucose 1-phosphate = alpha-D-glucose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:23536"/>
            <dbReference type="ChEBI" id="CHEBI:58225"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="EC" id="5.4.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.2" evidence="5"/>
    <dbReference type="EMBL" id="JAMZMK010008654">
        <property type="protein sequence ID" value="KAI7739238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CD20"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004614">
        <property type="term" value="F:phosphoglucomutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03085">
        <property type="entry name" value="PGM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.50:FF:000002">
        <property type="entry name" value="Phosphoglucomutase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.120.10:FF:000004">
        <property type="entry name" value="Phosphoglucomutase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.120.10:FF:000005">
        <property type="entry name" value="Phosphoglucomutase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.120.10:FF:000009">
        <property type="entry name" value="Phosphoglucomutase, cytoplasmic 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.120.10">
        <property type="entry name" value="Alpha-D-Glucose-1,6-Bisphosphate, subunit A, domain 3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.50">
        <property type="entry name" value="Alpha-D-phosphohexomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005844">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016055">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I/II/III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005845">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005846">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036900">
        <property type="entry name" value="A-D-PHexomutase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016066">
        <property type="entry name" value="A-D-PHexomutase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005841">
        <property type="entry name" value="Alpha-D-phosphohexomutase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045244">
        <property type="entry name" value="PGM"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005737">
        <property type="entry name" value="PRK07564.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22573:SF2">
        <property type="entry name" value="PHOSPHOGLUCOMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22573">
        <property type="entry name" value="PHOSPHOHEXOMUTASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24947">
        <property type="entry name" value="PGM1_C_vert_fung"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02878">
        <property type="entry name" value="PGM_PMM_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02879">
        <property type="entry name" value="PGM_PMM_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02880">
        <property type="entry name" value="PGM_PMM_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00509">
        <property type="entry name" value="PGMPMM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55957">
        <property type="entry name" value="Phosphoglucomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53738">
        <property type="entry name" value="Phosphoglucomutase, first 3 domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00710">
        <property type="entry name" value="PGM_PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="6" id="KW-0313">Glucose metabolism</keyword>
    <keyword evidence="10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="17">
        <location>
            <begin position="17"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="18">
        <location>
            <begin position="200"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="19">
        <location>
            <begin position="317"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF02878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF02879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF02880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAI7739238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="582" mass="62960" checksum="9CB920C7FBBBE8FF" modified="2024-05-29" version="1">MASFTVNRVETSPIEGQKPGTSGLRKKVKVFTQPHYLHNFVQSTFNALSTENVKGSTLVVSGDGRYYSKDAIQIIIKMAAANGVRRVWVGQNGLLSTPAVSAVVRERVGADGAKANGAFILTASHNPGGPNEDFGIKYNMGNGGPAPEGITDKIFENTKTIKEYFIAEGLPDVDISAIGISNFSGPDGQFDVDVFDSSSDYVKLMKSIFDFQSIKKLITSPQFSFCYDALHGVAGAYANRIFVEELGAKESSLLNCVPKEDFGGGHPDPNLTYAKELVARMGLGKNPDSNPPEFGAAADGDADRNMILGKRFFVTPSDSVAIIAANAVQSIPYFSSGLKGVARSMPTSAALDVVAKSLNLKFFEVPTGWKFFGNLMDAGLCSVCGEESFGTGSDHIREKDGIWAVLAWLSILAHKNKDNLNGGNLVTVEDIVKQHWATFGRHYYTRYDYENVDAGAAKELMAHLVKLQSSISDVNEIIKGIRPDVANVASADEFEYKDPVDGSISKHQGIRYLFEDGSRLVFRLSGTGSEGATIRLYIEQYEKDSSKTGRDSQEALAPLVEVALKLSKMQEFTGRSAPTVIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CDG9</accession>
    <name>A0AAD5CDG9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_003936</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008627">
        <property type="protein sequence ID" value="KAI7739439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CDG9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7739439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="248" mass="28647" checksum="24A16F4C35F40BF8" modified="2024-05-29" version="1">MFYEFGIFSTFYGGKEMPDWISCRSEGSSISFTIPSSSKKKLRGLNFICMDAFTYGDSYKRPYVKISNITKNLTWIYNHYVIWVKALEADSEDGEEYHVCVLSHWMFGPDEMKVGDHITITVEQIKNYVVRSGGKESYIPRIRTKACGIGFVYEDDIDEKTEEEDALGYYKSWNHIIGGDLSGFQLTTGEYILEFCNFRRDSVELVEYCPFIDGSAIYKEEKMWFKAFSGKKKSDEDVQVPRDVGNAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CDR2</accession>
    <name>A0AAD5CDR2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">beta-ketoacyl-[acyl-carrier-protein] synthase I</fullName>
            <ecNumber evidence="2">2.3.1.41</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_016166</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the thiolase-like superfamily. Beta-ketoacyl-ACP synthases family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.41" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010008738">
        <property type="protein sequence ID" value="KAI7738739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CDR2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009570">
        <property type="term" value="C:chloroplast stroma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004315">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00834">
        <property type="entry name" value="KAS_I_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000036">
        <property type="entry name" value="3-oxoacyl-[acyl-carrier-protein] synthase II chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017568">
        <property type="entry name" value="3-oxoacyl-ACP_synth-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000794">
        <property type="entry name" value="Beta-ketoacyl_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018201">
        <property type="entry name" value="Ketoacyl_synth_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014031">
        <property type="entry name" value="Ketoacyl_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014030">
        <property type="entry name" value="Ketoacyl_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020841">
        <property type="entry name" value="PKS_Beta-ketoAc_synthase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03150">
        <property type="entry name" value="fabF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004970">
        <property type="entry name" value="PRK06333.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005589">
        <property type="entry name" value="PRK07314.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11712:SF332">
        <property type="entry name" value="3-OXOACYL-[ACYL-CARRIER-PROTEIN] SYNTHASE II, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11712">
        <property type="entry name" value="POLYKETIDE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00109">
        <property type="entry name" value="ketoacyl-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02801">
        <property type="entry name" value="Ketoacyl-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00825">
        <property type="entry name" value="PKS_KS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00606">
        <property type="entry name" value="KS3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52004">
        <property type="entry name" value="KS3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041946996" description="beta-ketoacyl-[acyl-carrier-protein] synthase I" evidence="11">
        <location>
            <begin position="23"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="domain" description="Ketosynthase family 3 (KS3)" evidence="12">
        <location>
            <begin position="75"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="20"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="33"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS52004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7738739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="485" mass="51824" checksum="D526D61324AFB41D" modified="2024-05-29" version="1">MAASSVMCTWIVAACMSISSSSSPNSLFPSSSKSKRSLSTTRRRRPLPGVAMAIAIQPSSMETTPLSKNKPPTKQRRVVVTGMGVETPIGNTPDQFYNNLLQGVSGITPIEAFDCSNYPTRIAGEIKNFSTDGWVAPKLAKRMDRFMLYMLTAGKKALADAGADEYDEMDKTRCGVLIGSAMGGMKVFNDAIEALRVSYRKMNPFCVPFATTNMGSAMLAMDLGWMGPNYSISTACATSNFCILNAANHIIRGEADMMLCGGSDAVIIPIGLGGFVACRALSERNTDPAKASRPWDSGRDGFVMGEGAGVLLLEELEHAKKRGAKIYAEFLGGSFTCDAYHMTEPHPQGAGVILCIEKALAQAGVSREDVNYINAHATSTPAGDLKEYHALLHCFGNNQELRVNSTKSMIGHLLGAAGAVEAVATVQAIRTGWIHPNINLENPDEGVDTKVLVGSTKERLKVKVGLSNSFGFGGHNSSILFAPFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CEQ6</accession>
    <name>A0AAD5CEQ6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SPX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_019490</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008390">
        <property type="protein sequence ID" value="KAI7740612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CEQ6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016036">
        <property type="term" value="P:cellular response to phosphate starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14481">
        <property type="entry name" value="SPX_AtSPX1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004331">
        <property type="entry name" value="SPX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031142">
        <property type="entry name" value="SPX_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45978">
        <property type="entry name" value="SPX DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45978:SF2">
        <property type="entry name" value="SPX DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03105">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51382">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SPX" evidence="1">
        <location>
            <begin position="1"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7740612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="246" mass="28731" checksum="775628E62E307939" modified="2024-05-29" version="1">MKFGKRLKQHVEETLPGWRDMYLNYKDLKKLVRLISSSLEYGKSEAEFVYLLNNEIDKFNMFFMEQEEDFIIRHKELQQRIKRVNESEPSSQEEYEEEMSRIRKDIVDFHGEMVLLVNYSNINYTGMAKILKKYDKRTGGLLRLAFIQKVLEQPFFTTELVSKLVKECETTIDELFPTAMVAVELKDATITMVGEGIFRNTVAALMTMKEIRKGSSTQSHFSLPALSLPDTELIRYFQLNSPIPSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CEV4</accession>
    <name>A0AAD5CEV4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_020864</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008368">
        <property type="protein sequence ID" value="KAI7740687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CEV4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015157">
        <property type="entry name" value="TMA7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28632">
        <property type="entry name" value="TRANSLATION MACHINERY-ASSOCIATED PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09072">
        <property type="entry name" value="TMA7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7740687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="121" mass="13857" checksum="0BFC86ED35D147FA" modified="2024-05-29" version="1">MESKCGKAKPLKQPKAEKKEYDEIDKANIQKKKDEEKALKELRAKAQKGALGAEYVGEINVESLRRSPHSTTLTPIENVHSWPHSVMYERESSVEKREVYPLHYGHLRSLVSLGDHFSDQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CFE2</accession>
    <name>A0AAD5CFE2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Expansin-like B1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_022634</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the expansin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008603">
        <property type="protein sequence ID" value="KAI7739609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CFE2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd22277">
        <property type="entry name" value="DPBB_EXLB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.760">
        <property type="entry name" value="Expansin, cellulose-binding-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="entry name" value="RlpA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007118">
        <property type="entry name" value="Expan_Lol_pI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007112">
        <property type="entry name" value="Expansin/allergen_DPBB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007117">
        <property type="entry name" value="Expansin_CBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036749">
        <property type="entry name" value="Expansin_CBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009009">
        <property type="entry name" value="RlpA-like_DPBB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31692">
        <property type="entry name" value="EXPANSIN-B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31692:SF92">
        <property type="entry name" value="EXPANSIN-LIKE B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03330">
        <property type="entry name" value="DPBB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01357">
        <property type="entry name" value="Expansin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01225">
        <property type="entry name" value="EXPANSNFAMLY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00837">
        <property type="entry name" value="DPBB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="Barwin-like endoglucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49590">
        <property type="entry name" value="PHL pollen allergen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50843">
        <property type="entry name" value="EXPANSIN_CBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50842">
        <property type="entry name" value="EXPANSIN_EG45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042059715" description="Expansin-like B1" evidence="2">
        <location>
            <begin position="26"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="Expansin-like EG45" evidence="3">
        <location>
            <begin position="51"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="Expansin-like CBD" evidence="4">
        <location>
            <begin position="169"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7739609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="258" mass="28376" checksum="885275D89E9BABC0" modified="2024-05-29" version="1">MAFSFHAVMFIASALIFSLLTQSQGDTTSSGSFIHSRAGYYPNSEEIGSETGRCGYGSFGAIINYGDVAAASDLYRDGVGCGACYQVRCTNVKDCSNEGVTVVITDQGSSDRTDFIMSQKAFRKMAQNSYAASSLMSQGIVDIEYRRVSCSYPKYNITIKIDESSDYPYYLAFVIWYQQGQKDITAVQLCETNNFNCKLLDRSYGSVFTTTSPPSGPLSLRMLFSSEDGDEKWVVPVNSIPENWKKGAVYDTGVQVEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CG64</accession>
    <name>A0AAD5CG64_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_026824</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Nudix hydrolase family. DCP2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008353">
        <property type="protein sequence ID" value="KAI7740730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CG64"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140933">
        <property type="term" value="F:5'-(N(7)-methylguanosine 5'-triphospho)-[mRNA] hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000290">
        <property type="term" value="P:deadenylation-dependent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03672">
        <property type="entry name" value="NUDIX_Dcp2p_Nudt20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.79.10:FF:000003">
        <property type="entry name" value="M7GpppN-mRNA hydrolase isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1050">
        <property type="entry name" value="Dcp2, box A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007722">
        <property type="entry name" value="DCP2_BoxA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036189">
        <property type="entry name" value="DCP2_BoxA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044099">
        <property type="entry name" value="Dcp2_NUDIX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23114">
        <property type="entry name" value="M7GPPPN-MRNA HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23114:SF17">
        <property type="entry name" value="M7GPPPN-MRNA HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05026">
        <property type="entry name" value="DCP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01125">
        <property type="entry name" value="DCP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140586">
        <property type="entry name" value="Dcp2 domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="9">
        <location>
            <begin position="87"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7740730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="316" mass="34995" checksum="D17DEC06132E11F4" modified="2024-05-29" version="1" fragment="single">MSSSGGLPRSSSTPLKDGIPPKELLDDLCSRFVLNVPKEDQQSFERILFLVEYAHWFYEDNSVENNPSLKSFTLKEFTSLNFTSYKVRVPVTGAIILDQTYERCVLVKGWKGTSWSFPRGKKNKDEEDDACAIREVLEETGFNVSKLINKDEYIEMTFGPQRVRLYIVGGVKDDTAFAPQTKKEISPPVAPKPDSPARALPVSSFSLLDSGIIPTSPIRNSDQLLACCPSPVDSRPLVPLFWWQEAMSLWKAKGSSSSGNNLVMTETPSTSNLASVSVPVSNSNNSLNKGPGQSFRNFQFDSGPILQAIETAFSRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CII8</accession>
    <name>A0AAD5CII8_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_007170</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007974">
        <property type="protein sequence ID" value="KAI7742486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CII8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45974:SF242">
        <property type="entry name" value="LEUCINE-RICH REPEAT PROTEIN KINASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45974">
        <property type="entry name" value="RECEPTOR-LIKE PROTEIN 55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7742486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="108" mass="11564" checksum="4BF9788D6A8D2EA2" modified="2024-05-29" version="1" fragment="single">TKSSNTGIIIGSVVGGFVVLLLIASAVTYGVYQKRQATKAKHNSPFATWGADNGSEAGDVPQLKGARWCSFEELRRCTNNFSEDNMIGSGGYGKVSKSYKLPNLLFGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CIS1</accession>
    <name>A0AAD5CIS1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">2-isopropylmalate synthase</fullName>
            <ecNumber evidence="2">2.3.3.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_017629</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 1/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the alpha-IPM synthase/homocitrate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.13" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010008155">
        <property type="protein sequence ID" value="KAI7741620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CIS1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003852">
        <property type="term" value="F:2-isopropylmalate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:L-leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07940">
        <property type="entry name" value="DRE_TIM_IPMS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000010">
        <property type="entry name" value="2-isopropylmalate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050073">
        <property type="entry name" value="2-IPM_HCS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002034">
        <property type="entry name" value="AIPM/Hcit_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054691">
        <property type="entry name" value="LeuA/HCS_post-cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000891">
        <property type="entry name" value="PYR_CT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10277:SF9">
        <property type="entry name" value="2-ISOPROPYLMALATE SYNTHASE 1, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10277">
        <property type="entry name" value="HOMOCITRATE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22617">
        <property type="entry name" value="HCS_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00682">
        <property type="entry name" value="HMGL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00815">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00816">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50991">
        <property type="entry name" value="PYR_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="3" id="KW-0432">Leucine biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyruvate carboxyltransferase" evidence="8">
        <location>
            <begin position="2"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7741620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="430" mass="47136" checksum="3550AB70C894E4CC" modified="2024-05-29" version="1" fragment="single">YVRILDTTLRDGEQSSGVTLTQKEKLDIARQLAKLGVDVIEAGFPAASESEVEVVRLIAQEVGNTTDNDIGGDHHVPVISCLARCTNKDIDMAWGAIKYAKYPRILMFIATSEIHMKYKLNMTKVEVLEKAKSMVHYAKALGCNDIQFCLEDATRSEREFLYEIIGQVIKAGATTIGIGDTVGYNLPGEYGQLIADIRANTPGVENVIISAHCHNDLGLANANTLQAASCGARQLEVTINGIGERAGNAALEEPEVFDGPAPKGTVWPNLDEPGRPEPHTHKSYKCFTIEKMNDFVCSRNSIKDHYKTVEEYTGMHVQPHKAIVGANAFAHQSGIHQDGVLKNKSTYEIMSPKDIGLHRSNGFGLTLGKLSGSHALKVKLSEVVYNAQLLIDPSSNQHFICCALNLGYDYDGKELNDIFSRFKSLAETKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CJ09</accession>
    <name>A0AAD5CJ09_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Pectate lyase</fullName>
            <ecNumber evidence="3 8">4.2.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_000854</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>Eliminative cleavage of (1-&gt;4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.</text>
            <dbReference type="EC" id="4.2.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="8">Binds 1 Ca(2+) ion. Required for its activity.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 2/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the polysaccharide lyase 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.2.2" evidence="3 8"/>
    <dbReference type="EMBL" id="JAMZMK010008207">
        <property type="protein sequence ID" value="KAI7741394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CJ09"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030570">
        <property type="term" value="F:pectate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018082">
        <property type="entry name" value="AmbAllergen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002022">
        <property type="entry name" value="Pec_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045032">
        <property type="entry name" value="PEL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31683">
        <property type="entry name" value="PECTATE LYASE 18-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31683:SF69">
        <property type="entry name" value="PECTATE LYASE 7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00544">
        <property type="entry name" value="Pectate_lyase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00807">
        <property type="entry name" value="AMBALLERGEN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00656">
        <property type="entry name" value="Amb_all"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0106">Calcium</keyword>
    <keyword evidence="7 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041771137" description="Pectate lyase" evidence="8">
        <location>
            <begin position="24"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="domain" description="Pectate lyase" evidence="9">
        <location>
            <begin position="121"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7741394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="395" mass="43794" checksum="E63A9F2D567F5366" modified="2024-05-29" version="1">MEVSRAVLVLCLVVVAIAQVSMAENQSTAIAIRRPLGHKRPKCVAYNPIDKCWRCDSRWAKNRKRLADCAQGFGHEAKGGIMGRIYVVTDSSDKNVIDPKPGTLRHAILQAEPLWITFSRNMKIALSQELIFTSNKTIDGRGFEIHVASGCGFMLQFVNNIIIHGIHMYDIVEGSGGMIRSAPNHVGRRGRSDGDAVCIFKSSQVWIDHCSFANSYDGLIDIVAASTDVTISNCHFVRHDKALLFGASDETPEDKIMKVTIAYNHFGKGLTQRLPAVRFGFVHIVNNGYTEWQSYAIGGADGATIISQGNRFLAANGAAKEVTHREKVPESVWRKWTWRSEGDLLLNGAYFRQSGNPNWAKKYKGYPLIPALPVEDVPNLTKFVGPLHCKARRPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CJ77</accession>
    <name>A0AAD5CJ77_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">Protein N-terminal glutamine amidohydrolase</fullName>
            <ecNumber evidence="3 8">3.5.1.122</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6 8">Protein NH2-terminal glutamine deamidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_005627</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Mediates the side-chain deamidation of N-terminal glutamine residues to glutamate, an important step in N-end rule pathway of protein degradation. Conversion of the resulting N-terminal glutamine to glutamate renders the protein susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule. Does not act on substrates with internal or C-terminal glutamine and does not act on non-glutamine residues in any position.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>N-terminal L-glutaminyl-[protein] + H2O = N-terminal L-glutamyl-[protein] + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:50680"/>
            <dbReference type="Rhea" id="RHEA-COMP:12668"/>
            <dbReference type="Rhea" id="RHEA-COMP:12777"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:64721"/>
            <dbReference type="ChEBI" id="CHEBI:64722"/>
            <dbReference type="EC" id="3.5.1.122"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2 8">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the NTAQ1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.122" evidence="3 8"/>
    <dbReference type="EMBL" id="JAMZMK010008175">
        <property type="protein sequence ID" value="KAI7741546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CJ77"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008418">
        <property type="term" value="F:protein-N-terminal asparagine amidohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070773">
        <property type="term" value="F:protein-N-terminal glutamine amidohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.620.10:FF:000001">
        <property type="entry name" value="Blast:Protein N-terminal glutamine amidohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.620.10">
        <property type="entry name" value="Protein N-terminal glutamine amidohydrolase, alpha beta roll"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037132">
        <property type="entry name" value="N_Gln_amidohydro_ab_roll_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039733">
        <property type="entry name" value="NTAQ1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023128">
        <property type="entry name" value="Prot_N_Gln_amidohydro_ab_roll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13035">
        <property type="entry name" value="PROTEIN N-TERMINAL GLUTAMINE AMIDOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13035:SF0">
        <property type="entry name" value="PROTEIN N-TERMINAL GLUTAMINE AMIDOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09764">
        <property type="entry name" value="Nt_Gln_amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein N-terminal glutamine amidohydrolase alpha beta roll" evidence="9">
        <location>
            <begin position="21"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU367082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF09764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7741546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="223" mass="25341" checksum="A9CAFDFF0888CDF8" modified="2024-05-29" version="1">MASILDSSESSASSDVSQFHHTPSYCEENVYLVCKKLCADGVADVSDLFVVFISNEHKHIPLWHQKASHRADGIILWDYHVICIQRGKEGKSVDLVWDLDSTLPFPSTLASYVSEGIRPSFELFSEFQRVFRVVHAPLFLRYFASDRRHMKDSEGNWMAPPPEYKAIVAEDGTVHNLNQYIEMSTKDVLKDIKDSYVNAVFTNQLGVLVGESQLEQFFSCISR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CJ83</accession>
    <name>A0AAD5CJ83_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_026576</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008125">
        <property type="protein sequence ID" value="KAI7741860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CJ83"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050667">
        <property type="entry name" value="PPR-containing_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47939">
        <property type="entry name" value="MEMBRANE-ASSOCIATED SALT-INDUCIBLE PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47939:SF7">
        <property type="entry name" value="REPEAT-CONTAINING PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12854">
        <property type="entry name" value="PPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="154"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7741860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="273" mass="31191" checksum="53FDC66B62B26FE8" modified="2024-05-29" version="1">MIANAFMEIKCSLIELRSVCFLGSVVVVITDGNRVDDGWHNFLSKIQRKVTICRREGLEPAVVAAIPTMKFDCEAFCSMEDAQKEVGEMERSGITPDVVTAEIGNGIWNLMVMRGCLPNLATFNARVQFLVCKGRAWQANSLMSMMRYLGIKPDEVTFNLVIKGFCRAGYIDMAKRVYSVLHDECYKPNARIYQTMMHYLCKEGEVDMAYTTCKNSMEKNWFPSVDSIYQLLQGLQTTRKIDKARFIVSLVHKRVPPLPVKQLRIMKSFLKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CJI6</accession>
    <name>A0AAD5CJI6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein CLT2, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_002746</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CRT-like transporter family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008148">
        <property type="protein sequence ID" value="KAI7741679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CJI6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013936">
        <property type="entry name" value="CRT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31326">
        <property type="entry name" value="PROTEIN CLT2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31326:SF1">
        <property type="entry name" value="PROTEIN CLT2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08627">
        <property type="entry name" value="CRT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="92"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="161"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="186"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="215"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="244"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="279"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="341"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="368"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="397"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7741679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="436" mass="46996" checksum="E71FE3DAA38A6998" modified="2024-05-29" version="1">MSFAFSVAQPNLALNGSKAPPIYLLHHHMNHPSFGHNNNNNNANGLGMVSYRRKIAPIQRLPNPTRFVVRSDSANNNTTSSQSQSSNSNNKVVFWSAVTLVLAVGNRVMYKLALVPMNDYPFFLAQLNTFGYVAIYFSILYMRHRAGIVTNEMLSIPKWRFAVIGMLEALGVVAGMSSAAKLPGPAIPILNQTFLLWQLGFSALLLGRRYSWNKISGCLLVAAGVVTAIASGSESGQMLSGVGLLWPIMMVVSSAFQAGATIIKEYVFIDAAKRLKGKLLDIFVVNSFGSGFQALFVLLFLPFLSNLKGIPIHELPSYIKSGAGCFLNVGPASSGCDGSPLLPLLYIVNNIAFNISILNLVKISSAVVSSLAVVLSVPTTIYILSLPLPYLPEGVTLSPFFLFGSVILVLGLILYNVPQHSNSKHGPGSDSDLDIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-10-08" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CKD9</accession>
    <name>A0AAD5CKD9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cell division cycle protein 48 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_005655</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007885">
        <property type="protein sequence ID" value="KAI7742815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034098">
        <property type="term" value="C:VCP-NPL4-UFD1 AAA ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031593">
        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097352">
        <property type="term" value="P:autophagosome maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051228">
        <property type="term" value="P:mitotic spindle disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030970">
        <property type="term" value="P:retrograde protein transport, ER to cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000004">
        <property type="entry name" value="Cell division control 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.330.10:FF:000001">
        <property type="entry name" value="Cell division control 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002861">
        <property type="entry name" value="Cell division control protein 48 homolog E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.20:FF:000003">
        <property type="entry name" value="Transitional endoplasmic reticulum ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000012">
        <property type="entry name" value="Transitional endoplasmic reticulum ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.20.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004201">
        <property type="entry name" value="Cdc48_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029067">
        <property type="entry name" value="CDC48_domain_2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF180">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 48 HOMOLOG D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02933">
        <property type="entry name" value="CDC48_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01072">
        <property type="entry name" value="CDC48_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="ADC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54585">
        <property type="entry name" value="Cdc48 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CDC48" evidence="7">
        <location>
            <begin position="81"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="6">
        <location>
            <begin position="192"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="6">
        <location>
            <begin position="418"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="653"/>
            <end position="685"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7742815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="685" mass="76170" checksum="34554BD2056C56EC" modified="2024-05-29" version="1">MSNQPESSDSKGGKKDFSTAILERKKAPNRLVVDEAVNDDNSVVCMHPETMEKLQLFRGDTILLKYGKRVHILPIDDTIEGVTGNLFDAYLKPYFLEAYRPVRKGDYFLVRGGMRSVEFKVIETDPAEYCVVAPDTEIFCEGEPVRREDEDRLDEIGYDDVGGVRKQMAQIRELVELPLRHPQLFKSIGVKPPKGILLYGPPGSGKTLIARAVANETGAFFFCINGPEIMSKMAGESESNLRKAFEEAEKNAPSIIFIDELDSXXXXFSVIDFDGRAEVSGTFGRLEVLRIHTKNMKLSDDVDLEKVGKDTHGYVGADLAALCTEAALQCIREKMDVIDLEDESIDAEILNSMAVTNEHFQTALGTSNPSALRETVVEVPNVSWEDIGGLENVKRELQETVQYPVEHPEKFEKFGMAPSKGVLFYGPPGCGKTLLAKAIANECQANFISIKGPELLTMWXXXXCFTYLIRGSSQGDAGGAADRVLNQLLTEMDGMSAKKTVFIIGATNRPDIIDPALLRPGRLDQLIYIPLPDEESRYQIFKSALRKSPISKDVDLRALARFTQGFSGADITEICQRACKYAIRENIEKDIEREKRISENPEAMEEDMDDEVAEIKAAHFEESMKFARRSVSDADIRKYQAFAQTLQQSRGIGSEFRFSETSGRTAASDPFAAPAGTVDDDDLYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CNC3</accession>
    <name>A0AAD5CNC3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNase III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_016142</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007703">
        <property type="protein sequence ID" value="KAI7743646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CNC3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004525">
        <property type="term" value="F:ribonuclease III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1520.10">
        <property type="entry name" value="Ribonuclease III domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000999">
        <property type="entry name" value="RNase_III_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036389">
        <property type="entry name" value="RNase_III_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14622">
        <property type="entry name" value="Ribonucleas_3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00535">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69065">
        <property type="entry name" value="RNase III domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50142">
        <property type="entry name" value="RNASE_3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase III" evidence="1">
        <location>
            <begin position="23"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7743646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="164" mass="17388" checksum="7B38A2ADF43178E4" modified="2024-05-29" version="1" fragment="single">LIIPHQVSAGSQSLTSSSFSLALQTLQTHIKYEFKNIGYLRRAMTHASYSEENNKAFSVLGESIIETTVALKLLKNDVDITSKDMSDRIAEISKVETSCAVDGMRLGLQNVVRVSSSTDSSTSSIVCGAFRSLFGAIALDTGKSDDAGDVFLAVHGGAGGSFSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CPA7</accession>
    <name>A0AAD5CPA7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cytochrome b561 and DOMON domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">M8C21_030361</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">AA19_3_7</strain>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">May act as a catecholamine-responsive trans-membrane electron transporter.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
        <text evidence="10">Binds 2 heme b groups non-covalently.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007584">
        <property type="protein sequence ID" value="KAI7744111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CPA7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08760">
        <property type="entry name" value="Cyt_b561_FRRS1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09629">
        <property type="entry name" value="DOMON_CIL1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1770:FF:000007">
        <property type="entry name" value="Cytochrome b561 and DOMON domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045265">
        <property type="entry name" value="AIR12_DOMON"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006593">
        <property type="entry name" value="Cyt_b561/ferric_Rdtase_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005018">
        <property type="entry name" value="DOMON_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017214">
        <property type="entry name" value="UCP037471"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23130">
        <property type="entry name" value="CYTOCHROME B561 AND DOMON DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23130:SF221">
        <property type="entry name" value="CYTOCHROME B561 AND DOMON DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03188">
        <property type="entry name" value="Cytochrom_B561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04526">
        <property type="entry name" value="DUF568"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037471">
        <property type="entry name" value="UCP037471"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00665">
        <property type="entry name" value="B561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50939">
        <property type="entry name" value="CYTOCHROME_B561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50836">
        <property type="entry name" value="DOMON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 10" id="KW-0249">Electron transport</keyword>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="8 10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 10" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042006458" description="Cytochrome b561 and DOMON domain-containing protein" evidence="13">
        <location>
            <begin position="22"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="206"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="236"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="277"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="306"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="341"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="DOMON" evidence="14">
        <location>
            <begin position="44"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b561" evidence="15">
        <location>
            <begin position="164"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="11">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <label>1</label>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="11">
        <location>
            <position position="241"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <label>1</label>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="11">
        <location>
            <position position="274"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <label>1</label>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="11">
        <location>
            <position position="310"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <label>1</label>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00053871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR037471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR037471-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAI7744111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="393" mass="42412" checksum="DEF869B3363CDCB2" modified="2024-05-29" version="1">MGKILHLLFLILISNSIISSAQDCRSYPFTNNAVYATCVSLPVQNSHLHWNYDSTNRTVDLAFRHTGVSTSNWVAWALNINGNGMVGAQALVALPNSNGSVRAYTSAVSGYTTTLQESRLSFDVPVIRAERVNGDMVIYATLVLPSGRTSFNQVWQMGPVSNGAPSAHPMGSENRNSVGTVDFVSGQTGAGGGVGGNVLHRRNTHGVLNAVSWGILMPMGAMVARYLRVFKVGHPAWFYIHVAIQATAYGVGVAGWGTGLKLGSDSVGIKYTTHRNIGITLFALGTLQVFALLLRPKPDNKYRTYWNIYHAGVGYATISLGITNVFKGLDILDPEKKWKRAYIGVLIALAAIAVLLEAFTWLIVLKRKKEDKQQGTNGAYASNGYGHGHSQAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CTC2</accession>
    <name>A0AAD5CTC2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_018553</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006649">
        <property type="protein sequence ID" value="KAI7747878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CTC2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="92"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7747878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="154" mass="17136" checksum="BB558C1A4DC5623B" modified="2024-05-29" version="1">MAKPIDQVHAPTVVKQDHNDRFIRGHLQPIVLIIFPLLFASIAGMIYLHKENKSLWNKNEAVIGLLLFLVSRQIATADEEEDSLVFSFFTDTFYFSALLAGGLLFVTALCEGRAAVAMRWSITAFVVVIIVVCLLIHKSSNSNSDNDNDSRSIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CU06</accession>
    <name>A0AAD5CU06_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_000362</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006571">
        <property type="protein sequence ID" value="KAI7748248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CU06"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35729">
        <property type="entry name" value="T1B9.12 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35729:SF1">
        <property type="entry name" value="T1B9.12 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="270"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="461"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="270"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="295"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="472"/>
            <end position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7748248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="529" mass="57694" checksum="133E2DCEB7AD0B5E" modified="2024-05-29" version="1">MRVFNESWCFCNGGAKSERTKGCIYSGKGPAMVTISTGGLFSGTGFLIHRNLLLTTHAILPSLASADVAEIRIQNAVGASLFPHRFFITSSVLDLTIVGLDTMDGDSNMLSQQPHYLKTCSKPSLNLGSIVYLLGYTENKELTVGEGKVVIATDNLIKVCTDGVTWSPGSAGFDSHGNLSFMVCDPMKLATSPNTKTTSASSSSTSSWKKDLPMQFGIPIPVICDWLNQHWEGSLDDLVNKPKLPLIRLMSTGQKSEQSCTSFTMRQVFKTTGPENDPITPSSINVTSRSKDQPGPSSSPVTNSCQDETPAHDQPVHVQGIPTPEIYESPKLTSSPFKKNKNTQIQLLDINFPPKTTRPIGYGPTLPVKTQSNSKTEADIVSMESINGAENVVQSCSSQGQVSEGQNGYISEGETTMYSAETAESRNYPSPKGGRLSQHQVGRSQSCVNYNRWGPIQASPVARGRSLEKQRSYMQSRKVVSQGATSQRSNDYYSPTVSSIMKKRNNLEQQWPNRSRRPTAVHSSPKWAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CVK8</accession>
    <name>A0AAD5CVK8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein FMP32, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_029138</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006402">
        <property type="protein sequence ID" value="KAI7749123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CVK8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000029">
        <property type="entry name" value="Coiled-coil domain-containing protein 90-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024461">
        <property type="entry name" value="CCDC90-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14360:SF20">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14360">
        <property type="entry name" value="PROTEIN FMP32, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07798">
        <property type="entry name" value="CCDC90-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="222"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7749123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="245" mass="27478" checksum="EB43F7EE368A1E5C" modified="2024-05-29" version="1">MSVVMKRLIVELGFQVKSSKKSNVWGFATTSALVSKQSPPSPYHHYTYTTTIRHLSHIVNPNNGKRVCLVDTLALVRSLEAQGVPSKQAEAITSAITEVLNDSLENVSHTFVSKADMQKTVMLQEANLSKFKSQVQSSQEHHFSMLQRETEKLRTDIDKMRSELRYEIDKVTAGQRLDLNLERGRIRDELANQSAETANLTNKLDREIHGLRAQLEAAKYDVIKYCIGTLISISAVGLAVIRILV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CWD2</accession>
    <name>A0AAD5CWD2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Flap endonuclease 1</fullName>
            <shortName evidence="15">FEN-1</shortName>
            <ecNumber evidence="15">3.1.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Flap structure-specific endonuclease 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">M8C21_030881</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">AA19_3_7</strain>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structures that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA. May be required for cell proliferation.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="15">Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="15">Nucleus</location>
            <location evidence="15">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Nucleus</location>
            <location evidence="15">Nucleoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Mitochondrion</location>
        </subcellularLocation>
        <text evidence="15">Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage.</text>
    </comment>
    <comment type="similarity">
        <text evidence="13 15">Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="15"/>
    <dbReference type="EMBL" id="JAMZMK010006391">
        <property type="protein sequence ID" value="KAI7749237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CWD2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008409">
        <property type="term" value="F:5'-3' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017108">
        <property type="term" value="F:5'-flap endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043137">
        <property type="term" value="P:DNA replication, removal of RNA primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09907">
        <property type="entry name" value="H3TH_FEN1-Euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09867">
        <property type="entry name" value="PIN_FEN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000009">
        <property type="entry name" value="Flap endonuclease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000015">
        <property type="entry name" value="Flap endonuclease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00614">
        <property type="entry name" value="Fen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036279">
        <property type="entry name" value="5-3_exonuclease_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023426">
        <property type="entry name" value="Flap_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008918">
        <property type="entry name" value="HhH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006086">
        <property type="entry name" value="XPG-I_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006084">
        <property type="entry name" value="XPG/Rad2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019974">
        <property type="entry name" value="XPG_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006085">
        <property type="entry name" value="XPG_DNA_repair_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081:SF9">
        <property type="entry name" value="FLAP ENDONUCLEASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081">
        <property type="entry name" value="FLAP ENDONUCLEASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00867">
        <property type="entry name" value="XPG_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00752">
        <property type="entry name" value="XPG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00853">
        <property type="entry name" value="XPGRADSUPER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00279">
        <property type="entry name" value="HhH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00484">
        <property type="entry name" value="XPGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00485">
        <property type="entry name" value="XPGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47807">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00841">
        <property type="entry name" value="XPG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00842">
        <property type="entry name" value="XPG_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 15" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11 15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="2 15" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5 15" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="8 15" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="7 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 15" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 15" id="KW-0540">Nuclease</keyword>
    <keyword evidence="12 15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="1 15" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="XPG N-terminal" evidence="17">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="XPG-I" evidence="16">
        <location>
            <begin position="147"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00056033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAI7749237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="285" mass="31650" checksum="7C105FDA0E723ECF" modified="2024-05-29" version="1">MGIKGLTKLLADNAPKSMKEQKFESYFGRKIAIDASMSIYQFLIVVGRSGTEMLTNEAGEVTSHLQGMFNRTIRLLESGLKPVYVFDGAPPDLKKQELAKRYSRREDATAGLDEAIKSGNKEDIEKFSKRTVKVTRQHNEDCKKLLRLMGVPVIEAPSEAEAQCAALCKADKACIFVYAVASEDMDSLTFGAPKFLRHLMDPSSRKTPVMEFDVSKVLEELNLTMDQFIDLCILCGCDYCDSIRGIGGQTALKLIRQHGSIESILENINRERGPMRETPNEGVAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CYT8</accession>
    <name>A0AAD5CYT8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Myotubularin phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_027507</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006268">
        <property type="protein sequence ID" value="KAI7749842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CYT8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106018">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004438">
        <property type="term" value="F:phosphatidylinositol-3-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030564">
        <property type="entry name" value="Myotubularin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010569">
        <property type="entry name" value="Myotubularin-like_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10807">
        <property type="entry name" value="MYOTUBULARIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10807:SF8">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-3-PHOSPHATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06602">
        <property type="entry name" value="Myotub-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51339">
        <property type="entry name" value="PPASE_MYOTUBULARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myotubularin phosphatase" evidence="1">
        <location>
            <begin position="47"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7749842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="140" mass="16195" checksum="FA863F15B23F281B" modified="2024-05-29" version="1" fragment="single">MRIIIFGFRPGTKQRRAVFAALMMGTRPASLWDLYAFTFGPSKYSNTNPKVRLVNEYYRLLGMGSLQSSIGTIEDGLFKLSNDWWRISDVNASYNMCTTYPFALLVPKAIKDSELLKACTFRARCRLPVISWCDKRKYWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CYV5</accession>
    <name>A0AAD5CYV5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">YqaJ viral recombinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_032801</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006451">
        <property type="protein sequence ID" value="KAI7748880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CYV5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd22343">
        <property type="entry name" value="PDDEXK_lambda_exonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017482">
        <property type="entry name" value="Lambda-type_endonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051703">
        <property type="entry name" value="NF-kappa-B_Signaling_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011604">
        <property type="entry name" value="PDDEXK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019080">
        <property type="entry name" value="YqaJ_viral_recombinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03033">
        <property type="entry name" value="phage_rel_nuc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46609">
        <property type="entry name" value="EXONUCLEASE, PHAGE-TYPE/RECB, C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46609:SF6">
        <property type="entry name" value="EXONUCLEASE, PHAGE-TYPE_RECB, C-TERMINAL DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09588">
        <property type="entry name" value="YqaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YqaJ viral recombinase" evidence="1">
        <location>
            <begin position="73"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7748880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="311" mass="35715" checksum="6042ED2C502F0C9D" modified="2024-05-29" version="1">MTNACIIRIHFFHHKATLCLPQRNKFQFNLFRPFPTCASSLASTSLVYNPQWKTLTLTSQLSPSGAPAQRSEEWFALRKDRLTTSTFSTALGLWKGKRRYELWCEKVFPSDTNSPVSLVTKHAMEWGVLNEPTAIEKYKKITGREVNSLGFATHSEDKFDWIGASPDGLLGNFTNPGILEVKCPFNKGKPATAKPWSTMPFYYMPQVQGQMEVMDRDWVDLYCWTEKGSTIFRVSRDEEYWKLIHGVLREFWWENVVPAREALLMGSEVEARKYEPTSVHKLTGLIIHKSIKLANESKLLCREVAGHVEFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CZ10</accession>
    <name>A0AAD5CZ10_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein TIFY</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Jasmonate ZIM domain-containing protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_028236</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Repressor of jasmonate responses.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="2">The jas domain is required for interaction with COI1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the TIFY/JAZ family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006396">
        <property type="protein sequence ID" value="KAI7749181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CZ10"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031347">
        <property type="term" value="P:regulation of defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000022">
        <property type="term" value="P:regulation of jasmonic acid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009611">
        <property type="term" value="P:response to wounding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018467">
        <property type="entry name" value="CCT_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040390">
        <property type="entry name" value="TIFY/JAZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010399">
        <property type="entry name" value="Tify_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33077:SF61">
        <property type="entry name" value="PROTEIN TIFY 3A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33077">
        <property type="entry name" value="PROTEIN TIFY 4A-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09425">
        <property type="entry name" value="Jas_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06200">
        <property type="entry name" value="tify"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00979">
        <property type="entry name" value="TIFY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51320">
        <property type="entry name" value="TIFY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1184">Jasmonic acid signaling pathway</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tify" evidence="3">
        <location>
            <begin position="53"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU369065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7749181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="141" mass="15921" checksum="E68027C4AD192B30" modified="2024-05-29" version="1">MSGIELNHSPPHKNLLHRRQSFRDIQGSISKMNPELVKTVIESGKKPTSVSLSMQETTPLTIFYNGIVSVYQVSQLQAETVIKFAYGKETPTSPSHHKLQTLKQGKLDFDESCSDLPIARKRSLQRFLKKRKGRVNLGAPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D2R5</accession>
    <name>A0AAD5D2R5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Calcineurin B-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_010918</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Acts as a calcium sensor. CBL proteins interact with CIPK serine-threonine protein kinases. Binding of a CBL protein to the regulatory NAF domain of a CIPK protein lead to the activation of the kinase in a calcium-dependent manner.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer. Interacts with CIPK.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the calcineurin regulatory subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005600">
        <property type="protein sequence ID" value="KAI7752838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D2R5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019900">
        <property type="term" value="F:kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045198">
        <property type="entry name" value="CNBL1-10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23056">
        <property type="entry name" value="CALCINEURIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23056:SF147">
        <property type="entry name" value="CALCINEURIN B-LIKE PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13833">
        <property type="entry name" value="EF-hand_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00450">
        <property type="entry name" value="RECOVERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="38"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="82"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="116"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="125"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU369080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7752838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="139" mass="15888" checksum="7EE008121E6FB429" modified="2024-05-29" version="1" fragment="single">MKNFFADRLFDTFDIKKNGVIEFDEFVRSLSVFHPDAPESDKIEFMFRLYDLKHNGFIERDELKEMVLALLREMNVDLADDAVDGILNKTILDADLNGDGKIDLEEWKTFGDHSSVSKLRDTYDSPESGSNSISRASTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D4A3</accession>
    <name>A0AAD5D4A3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein MEMO1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_011658</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MEMO1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005552">
        <property type="protein sequence ID" value="KAI7753089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D4A3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07361">
        <property type="entry name" value="MEMO_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.830.10">
        <property type="entry name" value="LigB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002737">
        <property type="entry name" value="MEMO1_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04336">
        <property type="entry name" value="AmmeMemoSam_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11060">
        <property type="entry name" value="PROTEIN MEMO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11060:SF0">
        <property type="entry name" value="PROTEIN MEMO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01875">
        <property type="entry name" value="Memo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7753089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="133" mass="15264" checksum="A3BF91FD931D72D3" modified="2024-05-29" version="1">MMDLNVDEAEHSMEMHLPYLVKVFRGHTVKVVPIMVGAVSADSEAMYGRLLAKYIDDPTNFFSVSSDFCHWGSRFNYTHYDKKHGPIYKSIEALDKMGMEIIETGDPDEFKQYLLETDNTICGRHPISVFLHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D777</accession>
    <name>A0AAD5D777_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">F-box/FBD/LRR-repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_006053</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010003865">
        <property type="protein sequence ID" value="KAI7754365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D777"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055411">
        <property type="entry name" value="LRR_FXL15/At3g58940/PEG3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31639:SF312">
        <property type="entry name" value="CYCLIN-LIKE F-BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31639">
        <property type="entry name" value="F-BOX PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24758">
        <property type="entry name" value="LRR_At5g56370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="14"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="F-box/LRR-repeat protein 15/At3g58940/PEG3-like LRR" evidence="2">
        <location>
            <begin position="105"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7754365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="333" mass="38064" checksum="59ABFD23E3426DDC" modified="2024-05-29" version="1">MALEASEFTPEDFISKMPDNVITSILHRLPLLDAVRTGILSRTWRFKWTMLTQLKFNEEFFLYLMKTKGVDSIGRIISRLLLNLNGAVTKTVFVPVILDVDDIRRLIMFLSSKGIKDLTITDWSAPQVKLPTHLFACLELTRLNIYGFCFNPPPGFHGFPNLVSLELRGGQFEGDDLGEFLTRVRLSFVFELTEDGATKKFPTAFTSIRALKLTRIDFGNRLMLSFALETIGHFLNLQTLDIISSSRHVDDPFPISIPEVDYNMTELRSVMFRCFKGSENEVYMIKYLLACSPSLKAIGILRDRCLQPVEELIYAKKLLKLHRASAEAEIDFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D794</accession>
    <name>A0AAD5D794_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Tryptophan synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_003720</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine = D-glyceraldehyde 3-phosphate + L-tryptophan + H2O</text>
            <dbReference type="Rhea" id="RHEA:10532"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:58866"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.2.1.20"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 5/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Tetramer of two alpha and two beta chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9 10">Belongs to the TrpA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010003020">
        <property type="protein sequence ID" value="KAI7754592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D794"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004834">
        <property type="term" value="F:tryptophan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009851">
        <property type="term" value="P:auxin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04724">
        <property type="entry name" value="Tryptophan_synthase_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000107">
        <property type="entry name" value="Tryptophan synthase alpha chain, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00131">
        <property type="entry name" value="Trp_synth_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018204">
        <property type="entry name" value="Trp_synthase_alpha_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002028">
        <property type="entry name" value="Trp_synthase_suA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00262">
        <property type="entry name" value="trpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43406:SF1">
        <property type="entry name" value="TRYPTOPHAN SYNTHASE ALPHA CHAIN, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43406">
        <property type="entry name" value="TRYPTOPHAN SYNTHASE, ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00290">
        <property type="entry name" value="Trp_syntA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00167">
        <property type="entry name" value="TRP_SYNTHASE_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0073">Auxin biosynthesis</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0822">Tryptophan biosynthesis</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00060788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7754592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="332" mass="35397" checksum="FE5879E4A6CD8AA4" modified="2024-05-29" version="1">MNNIMSSSIASKSVSFLHLQHTNTNNKPNSLFRLQSPNILPFPHKSLSLTSSSSFKPPMATLAVSSPTLGLSETFSKLKQDGKVAFIPYITAGDPDLSTTAEALKVLDNCGSDIIELGVPYSDPLADGPVIQAAATRALARGANLDAILKMLKEVIPQLSCPIALFTYYNPILKRGVEKFMVTVKEVGVHGLVVPDVPLEETELLRQEASKHNIELVLLTTPTTPRDRMKAIVEATEGFLYLVSSIGVTGARASVSEKVQSLLQEIKAESSKPVAVGFGISKPEHVKQVAGWGADGVIVGSAVVKILGEAGSPEEGLKKLEEFTKSLKAALV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D8G5</accession>
    <name>A0AAD5D8G5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Pterin-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">M8C21_032288</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">AA19_3_7</strain>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(7,8-dihydropterin-6-yl)methyl diphosphate + 4-aminobenzoate = 7,8-dihydropteroate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19949"/>
            <dbReference type="ChEBI" id="CHEBI:17836"/>
            <dbReference type="ChEBI" id="CHEBI:17839"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:72950"/>
            <dbReference type="EC" id="2.5.1.15"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>6-hydroxymethyl-7,8-dihydropterin + ATP = (7,8-dihydropterin-6-yl)methyl diphosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11412"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:44841"/>
            <dbReference type="ChEBI" id="CHEBI:72950"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.6.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor biosynthesis; tetrahydrofolate biosynthesis; 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate from 7,8-dihydroneopterin triphosphate: step 4/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cofactor biosynthesis; tetrahydrofolate biosynthesis; 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">In the C-terminal section; belongs to the DHPS family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="15">In the N-terminal section; belongs to the HPPK family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005404">
        <property type="protein sequence ID" value="KAI7753545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D8G5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003848">
        <property type="term" value="F:2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004156">
        <property type="term" value="F:dihydropteroate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046656">
        <property type="term" value="P:folic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00739">
        <property type="entry name" value="DHPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00483">
        <property type="entry name" value="HPPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.20:FF:000006">
        <property type="entry name" value="Dihydropteroate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.560:FF:000003">
        <property type="entry name" value="Folate synthesis bifunctional protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.560">
        <property type="entry name" value="7,8-Dihydro-6-hydroxymethylpterin-pyrophosphokinase HPPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.20">
        <property type="entry name" value="Dihydropteroate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045031">
        <property type="entry name" value="DHP_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006390">
        <property type="entry name" value="DHP_synth_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011005">
        <property type="entry name" value="Dihydropteroate_synth-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000550">
        <property type="entry name" value="Hppk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035907">
        <property type="entry name" value="Hppk_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000489">
        <property type="entry name" value="Pterin-binding_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01496">
        <property type="entry name" value="DHPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01498">
        <property type="entry name" value="folK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20941">
        <property type="entry name" value="FOLATE SYNTHESIS PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20941:SF1">
        <property type="entry name" value="FOLIC ACID SYNTHESIS PROTEIN FOL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01288">
        <property type="entry name" value="HPPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00809">
        <property type="entry name" value="Pterin_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55083">
        <property type="entry name" value="6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase, HPPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51717">
        <property type="entry name" value="Dihydropteroate synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00792">
        <property type="entry name" value="DHPS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00793">
        <property type="entry name" value="DHPS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00794">
        <property type="entry name" value="HPPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50972">
        <property type="entry name" value="PTERIN_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0289">Folate biosynthesis</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pterin-binding" evidence="16">
        <location>
            <begin position="229"/>
            <end position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00009951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00061359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAI7753545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="509" mass="56470" checksum="0D0B11535BDD095F" modified="2024-05-29" version="1">MNIFKQLLSSRLARTRTRVCTSYRASSSFFHFSSDASIQVHSQEQEVVIALGSNVGDRLNNFNEALSQMKKSGIEITRHACLYETEPAYVTDQPLFLNSAIRGFTKLGPHELLSALKKIEKEMGRTKGIRYGPRPIDLDILFYGKHRINSEILTVPHERIFERPFVMAPLVDLLGSDIDNDTVLCWHSFSKRGLFESWEELGGESLIGKDGLRRVLPVNNRLWDWSKKTSVMGILNLTPDSFSDGGKFDSVGSAISRVRTMISEGADIIDLGAQSTRPMATKISVQEELDRLIPVLEKILELPKIEGKLLSIDTFYSEVALEAIKKGAHIINDVSGGNLDSDMFRVVANLDVPYIAMHMRGDPSTMQNSENLKYDDVCKEVGDELYERVRTAELCGVPAWRIILDPGIGFSKKTEDNLDILMGLKRIRSEIARKSLGMSRAPLLIGPSRKRFLGEICGRASAVERDPGTVAAVTCAVLGGANVVRVHNVGDNADAVKLCDSMLDRVGRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2024-10-02" version="2" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D9R2</accession>
    <name>A0AAD5D9R2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_004459</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010002642">
        <property type="protein sequence ID" value="KAI7754731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7754731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="92" mass="10101" checksum="41C19FDC212C52BE" modified="2024-05-29" version="1">MNLTISGACALRFQEFSAVYRLTVVYCAHISSMAEDGVIVPTQGKWKLPIFVFFKDLRLVLKMDSLGKEILLIAVPAAMALAADPXXXXLIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DAQ7</accession>
    <name>A0AAD5DAQ7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">M8C21_004513</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">AA19_3_7</strain>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010002744">
        <property type="protein sequence ID" value="KAI7754685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DAQ7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000142">
        <property type="entry name" value="Cysteine-rich receptor-like protein kinase 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.430.20">
        <property type="entry name" value="Gnk2 domain, C-X8-C-X2-C motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038408">
        <property type="entry name" value="GNK2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002:SF1050">
        <property type="entry name" value="CYSTEINE-RICH RECEPTOR-LIKE PROTEIN KINASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002">
        <property type="entry name" value="RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE SD1-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="54"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="118"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAI7754685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="213" mass="23562" checksum="822A514C9F8AF2C7" modified="2024-05-29" version="1">MTPIGRLEKNCSGKQGARVLAASCNVRFEIYEFLQFKDEGLTSNSGKKKSSSKIVAAIVAIVVSLVVIVAVWYFLVVKKQRRTITSSDLKDESDETAMISEQSLQYDLGTIEEATTNFSSENKIGEGGFGEVYKVRIFSLLAGTLSCGLKIAVKRLSKSSGQGLLEFKNEVSLLAKLQHRNLVRLLGFCLNAEEKILIYEYVANHSLDFFLFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DEC8</accession>
    <name>A0AAD5DEC8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">50S ribosomal protein L18</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_014659</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000008">
        <property type="protein sequence ID" value="KAI7758297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DEC8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008097">
        <property type="term" value="F:5S rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.100:FF:000006">
        <property type="entry name" value="50S ribosomal protein L18-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005484">
        <property type="entry name" value="Ribosomal_uL18_bac/plant/anim"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12899">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L18, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12899:SF21">
        <property type="entry name" value="OS09G0549700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00861">
        <property type="entry name" value="Ribosomal_L18p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042224150" description="50S ribosomal protein L18" evidence="4">
        <location>
            <begin position="22"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7758297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="216" mass="23555" checksum="702C00A998D050FC" modified="2024-05-29" version="1">MVESSLVFASLRVLSTTFCLSLELRVIAPFTVDILMRVDQRKQKQPLALAPATCCTCTVPAHPVQPVLPLPPLVASAGLLACLTSLGGYKTMTVLKRYVLRLFMSLKYITANVVDRNNGRIVATASTVEHSIKNSLECGRTCNAKAASIVGEVLAMRLRVEGLESGQERGIHVDINKELEKKDFKNRTKVWAVVNSLKNNGVKLVLDENNDGSHPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DEP6</accession>
    <name>A0AAD5DEP6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_005374</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the major facilitator superfamily. Phosphate:H(+) symporter (TC 2.A.1.9) family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000412">
        <property type="protein sequence ID" value="KAI7756395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DEP6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015145">
        <property type="term" value="F:monosaccharide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17361">
        <property type="entry name" value="MFS_STP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000002">
        <property type="entry name" value="Sugar transport protein 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044778">
        <property type="entry name" value="MFS_STP/MST-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045262">
        <property type="entry name" value="STP/PLT_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23500:SF357">
        <property type="entry name" value="IP12678P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23500">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 2, FACILITATED GLUCOSE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="6" id="KW-0769">Symport</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="83"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="113"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="138"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="172"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="205"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="286"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="322"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="351"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="385"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="427"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="453"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="12">
        <location>
            <begin position="28"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7756395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="521" mass="56899" checksum="54BCB1C3FE888AEA" modified="2024-05-29" version="1">MAGGGLAVASAAGGVEFEAKITPIVIISCIMAATGGLMFGYDVGVSGGVTSMPDFLKKFFPVVYKRTNSGDLGSNYCKYDNQGLQLFTSSLYLAGLTATFFASYTTRRLGRRLTMLIAGVFFLIGVVLNAAAQDLAMLIAGRILLGCGVGFANQAVPVFLSEIAPTRIRGGLNILFQLNVTIGILFANLVNYGTAKIKGGWGWRLSLGLAGIPAILLTIGALLVVDTPNSLIERGKLEQGKAVLKKIRGTDNVEPEYLELVEASRIAKEVKHPFRNLLLSKNRPPLIIAVALQFFQQFTGINAIMFYAPVLFSTLGFENSASLYSAVITGAVNVLSTIVSVYSVDKVGRRFLLLQAGVQMFLSQVVIAIVLGLKVTDKSDNMGTGFAVLVVIMICTYVAAFAWSWGPLGWLIPSETFPLETRSAGQSVTVCINLLFTFVIAQAFLSMLCHFKYGIFLFFSGWVFVMSIFVWFLVPETKNIPIEEMTERVWKKHWLWKRYMVDDDDEVYVQDDLAKKNGFGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G329</accession>
    <name>A0AAD5G329_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mitochondrial proton/calcium exchanger protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Leucine zipper-EF-hand-containing transmembrane protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">M8C21_007998</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">AA19_3_7</strain>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LETM1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011702">
        <property type="protein sequence ID" value="KAI7726342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G329"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030003">
        <property type="term" value="P:intracellular monoatomic cation homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033122">
        <property type="entry name" value="LETM1-like_RBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044202">
        <property type="entry name" value="LETM1/MDM38-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14009">
        <property type="entry name" value="LEUCINE ZIPPER-EF-HAND CONTAINING TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14009:SF1">
        <property type="entry name" value="MITOCHONDRIAL PROTON_CALCIUM EXCHANGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07766">
        <property type="entry name" value="LETM1_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51758">
        <property type="entry name" value="LETM1_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0050">Antiport</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="218"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="Letm1 RBD" evidence="15">
        <location>
            <begin position="264"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="14">
        <location>
            <begin position="613"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="14">
        <location>
            <begin position="649"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="473"/>
            <end position="524"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAI7726342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="689" mass="78849" checksum="046E72C77B2A8526" modified="2024-05-29" version="1">MASGVILRRKRYTFSFLKQGFLQSEVRGSPYSYSVVDIANKGFGGFLVREYIAQNACRIPSSGLWNVQTPFKGVGSVRCLSVVPNLDVQQPQKQVKEASPADCDDAVEDLSVVKAKAKAKQVQESGKDVKFIVNKVRDMLLWIGQALRAIASMSRKDWVVKLHHWKDEVKNTLQHYWLGMRLLWADVRISSRLLLKLSSGRSLTRRERQQLTRTTADIFRLVPFAVFIIVPFMEFLLPVFLKLFPNMLPSTFQDKMKEQEALKKRLNARMEYAKFLQETVQEMAKEVQNSRSGDIKRTAKDLDEFMNKVRRGTRVSNDEILGFAKLFNDELTLDNISRPRLVNMCKYMGISPFGTDAYLRYMLRKRLQEIKDDDKMIQAEGVDSLSEEELRQACRDRGLLGLRSVDEMRQQIRDWLDLSLNHSVPSSLLILSRAFSVSGKIKPEEAVQATLSSLPDEVVDTVGITALPSEDRVAEKRRKLEFLEMQEELIKEEEKEEAEQVRMKDSIEKQKDVALEEMVTLTTEEAKEHSKKSNKQEQLSELSRALAVLASASSVSREREEFLTLVNKEIDLYNSMVEKEGTDGEEESKKAYRAARTDTDHAAEMAIEKEIDEVDAKIGDRWRLLDRDYDGKVTPEEVASAAMYLKDTLGKEGLQELISNFSKDRDGKINVEDIIKLGSQKDESEDRRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G338</accession>
    <name>A0AAD5G338_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ER membrane protein complex subunit 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_012160</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EMC4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011456">
        <property type="protein sequence ID" value="KAI7727059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G338"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009445">
        <property type="entry name" value="TMEM85/Emc4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19315">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06417">
        <property type="entry name" value="EMC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="158"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="11"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="568"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7727059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="568" mass="64541" checksum="A4EC045B6A72D98A" modified="2024-05-29" version="1" fragment="single">VSWCSPYNDEPSSLPPSTTTTAPPQSCQSTINTTKSSSRALIIPAHDLTMGELGKRKRLAWGRRRALITTDQFQHRRLVPSFLKQIGFSRLNITHQASQAMTLSSMFPTFAARICSDAEIGVAHFGHCLLNLPITVDKSKAGISVFLRLLRSYKPFCYLHYFVFCYFSLVFYLHVSFSRLFVSHTWLLLRFRLLSSSASNLTICIHVWEGIIDDRGWCVSDDQFDLGNREEERQRRKPANHFLVLRRSFRSGDAAEELLRKNRSLRCNGFMRAEAPIQKGPNYESSHQCNTSTPVWEMTNFPSICTSQHINFPIIFGYKVYRLAFHIFASVIHFSSMQILTCPIVEERVYNLTVCLFCSGLSNIPGVVSIAALWLSEATVGTLDHIQKEGWHWTGTLVRYGLDSGWIDAQISTSIQRRYLLHIYIIIFKMEFTYNIYSRTIKAAVEFIDSSSTPCSRNIPDPLGFTRVPDDQDDSTLSHQKKDAKANRKSKKAWEVVQALFKNLMVMGFMMWTAGSKVHLFSIGIMFSALWQLLSALQGVGKVFAPYKDSKVDLLTLKLLFIALNLGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G3X8</accession>
    <name>A0AAD5G3X8_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_026933</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011404">
        <property type="protein sequence ID" value="KAI7727272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G3X8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7727272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="141" mass="15828" checksum="B5377D78FE17F589" modified="2024-05-29" version="1">MRGLTTAKTVAYDPKPQAMAKTNRSAEHNCNIRLLGRRLRPDQGQTTAITYGCEAVSYSGFLPPLRFSLFLIFNACLTPLTGCNLVLLPRYMQQHNEVELSALGMGDHVVKKRKAQWHGLAEASRVFVFREEKAIFAPHLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G5H5</accession>
    <name>A0AAD5G5H5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_012895</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011254">
        <property type="protein sequence ID" value="KAI7728203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G5H5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24296">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0349">Heme</keyword>
    <keyword evidence="5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7728203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="528" mass="60567" checksum="17B9C73AC37EEA48" modified="2024-05-29" version="1">MVGDINYLLIILSLSFLTLSTLFYRLTRRSYSAVKTPCPQSYPLIGNLIGFLLNRHRYHGWVTDMLSITPTLTLRVNSFLGLSHSIWTADPTNLEHILGSNFPNYVKSSRYTSVMQELLGSGIYNSDGILWASHRKITGPEFAVVSLKTFICNTVQSQLSKSLIPLLLSAGSDTFDLQHVLRKFSFDTICNIAFGVDPWSSFAQAFDAALEHTSNRFMSPFPVVWKLKRYFNIGDERKYKETIKIVNQFAMDVIKSKDAQNCYEINKDLLSKFMVFSWGMGFEDEERSKFLRDTIINFVFADGHPHCKHMIYNEFSMLTTSSTHIRPLNLTLDDLKRLNYLHAALSESMRLFPPMPINSRSVVNDDILPDGTFVHKGWFVDYSVYAMGRMESLWGYDCREFKPERWLDGNGVYRQPDDMYVYPVFNGGLRMCIGKEVAYIQMKLVVVAVMHEFEIEYPVFHGGFRLCLGKEMAYLQMKSVVVAVMHEFEIEVVGGGGTPERMVDPPYSLSLLLNMKNGLHVRLKKRQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G5W0</accession>
    <name>A0AAD5G5W0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_015157</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010990">
        <property type="protein sequence ID" value="KAI7729492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G5W0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004559">
        <property type="term" value="F:alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.110.10">
        <property type="entry name" value="Glycoside hydrolase 38, N terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027291">
        <property type="entry name" value="Glyco_hydro_38_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050843">
        <property type="entry name" value="Glycosyl_Hydrlase_38"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11607">
        <property type="entry name" value="ALPHA-MANNOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11607:SF3">
        <property type="entry name" value="LYSOSOMAL ALPHA-MANNOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7729492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="164" mass="19152" checksum="9D5A17F16F8515C9" modified="2024-05-29" version="1" fragment="single">GQQRLKATVVRFDSLFFGRIDSQDKAKRKADKNLEVIWWASKSLGAFPHNYEPPPGGFNFEVNDDSPIVQNSFKLKHAFSLWFISTKRIVVEGEWKRVCDFLIKLKRVIPRKGSNLFAYKYAASEDQLKDNPNVALFFQEQELYQGKEMNLHFTKNDIFTSKNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G6P8</accession>
    <name>A0AAD5G6P8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">SelT-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_021468</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010726">
        <property type="protein sequence ID" value="KAI7730584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G6P8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004791">
        <property type="term" value="F:thioredoxin-disulfide reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011893">
        <property type="entry name" value="Selenoprotein_Rdx-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019389">
        <property type="entry name" value="Selenoprotein_T"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02174">
        <property type="entry name" value="CXXU_selWTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13544">
        <property type="entry name" value="SELENOPROTEIN T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13544:SF0">
        <property type="entry name" value="THIOREDOXIN REDUCTASE-LIKE SELENOPROTEIN T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10262">
        <property type="entry name" value="Rdx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042158635" description="SelT-like protein" evidence="4">
        <location>
            <begin position="25"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="33"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="47"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7730584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="226" mass="24945" checksum="F22D175DBC2A7118" modified="2024-05-29" version="1">MAMDRSQLLLVGLPLFLFCSDVFNLFAPTPSKPNHNHNHNHNHIPSLQPQQQQQQPLLDFPAQKASGGGIGLGNTVQIDFCASCSYRGTAQTMKNMLESAFPGLNVVLANYPPPLPKRLLAKLVPVVQFGMIGVVMGGEHIFPRLGFATPPAWYYSLRANRFGTISSTWLMGNFLQGFLQSSGAFEVSINGEQVYSKLQEKRFPGEIELRDAIGKRIVNTRVVDGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G7S0</accession>
    <name>A0AAD5G7S0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TF-B3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_020328</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010412">
        <property type="protein sequence ID" value="KAI7731649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G7S0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.330.10">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003340">
        <property type="entry name" value="B3_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015300">
        <property type="entry name" value="DNA-bd_pseudobarrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044976">
        <property type="entry name" value="FIPS5/FIPS3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36884">
        <property type="entry name" value="FIP1[III]-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36884:SF1">
        <property type="entry name" value="FIP1[V]-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101936">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50863">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TF-B3" evidence="6">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7731649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="174" mass="19361" checksum="8AE7A1D4CA42F72E" modified="2024-05-29" version="1">MQPLPYDNARHHFQNKDLNTPFLIRFGTAHEWLVRVEKLFDDYYFTDGWQQLLKDLNIQIGDTLVFEKDTETSFNIIRFPWQVVFQYVRPGAAPIPGAGPAAVGGAPGQVRPPGGLLPFAGRGRGEWRPAGINKNVPPNMQKNFHPGYGMQGWGNNGAGRGFGSGLDFTLPSHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G830</accession>
    <name>A0AAD5G830_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Enhanced disease resistance 4-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_013338</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010593">
        <property type="protein sequence ID" value="KAI7730916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G830"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900150">
        <property type="term" value="P:regulation of defense response to fungus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040244">
        <property type="entry name" value="EDR4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055126">
        <property type="entry name" value="EDR4-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31105">
        <property type="entry name" value="EXTRA-LARGE G-PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31105:SF42">
        <property type="entry name" value="OS02G0258300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22910">
        <property type="entry name" value="EDR4-like_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enhanced disease resistance 4-like N-terminal" evidence="2">
        <location>
            <begin position="14"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="670"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7730916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="670" mass="75618" checksum="6EE3785BB7981670" modified="2024-05-29" version="1" fragment="single">ENKFKKMEEEGGPLRLVRCPKCENLLPELNEYSVYQCGGCGAVLQGKKLIPTSDKRSLETSDNENGTRVEPSTKEEVSLESSSQMKTESDEFEVGRRQGRMVHEKVPNLIPSSSSRKEKREFFADSDKNRRRRLDFEPIRSRPVGFQRNASTVEKGRFGNLPYSNEGPSSSHLSSFNSFGDHVTKFQDLEKDRAELLKKLDELRTQLSRSCNVTESPNGRLSGSYAWPHQPPRHQYLSPTFHNPFLGYNQHDTLLHRHSCSCSQCYNTHWQRIPPKVNPFSPFTSQETSYPLPEPLTYGPRHLPHHVRSLDMGNDGFGRRYPRRRLLKIPKKVTFNGKSDHKTIKCAVCSALISLRLEDNKIVASFSSTVNQQKVTKRKSNRSPHSSLDYDSSFNFQLTEAEQNVSAAGAEMKDEQIPDSAIDKPSLPVPVLESQNDVLTKSQNDVLTESRRTNQERVINDHDNKKLLKDALVATETDVSLTEYLNFDAVNKENDHSRSKKVGNESFFASLLKKRLKDFSRSSSGVEKVRSNVFVNGQLLTNRAVKRAEKLAGPIQSGEYWYDSLAGFWGVMNQPCLGIIPPFIEEFDHPMPKNCAAGNTGVFVNGRELNQKDLDLLASRGLPTTKDRSYIVEISGKVVDEDTGEELDALGKLAPTVERVKHGFGMKVPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G887</accession>
    <name>A0AAD5G887_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_029408</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010153">
        <property type="protein sequence ID" value="KAI7732805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G887"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041266">
        <property type="entry name" value="EDS1_EP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002921">
        <property type="entry name" value="Fungal_lipase-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044603">
        <property type="entry name" value="SAG101-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46898:SF3">
        <property type="entry name" value="FUNGAL LIPASE-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46898">
        <property type="entry name" value="SENESCENCE-ASSOCIATED CARBOXYLESTERASE 101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18117">
        <property type="entry name" value="EDS1_EP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01764">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0611">Plant defense</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fungal lipase-type" evidence="7">
        <location>
            <begin position="120"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="EDS1 EP" evidence="8">
        <location>
            <begin position="365"/>
            <end position="569"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF18117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7732805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="582" mass="66478" checksum="E8ED9E5111AE9FE7" modified="2024-05-29" version="1">MDSSLDQHITCRFDSEVELGKFTGSTDAIHDAHRAILETNEPYKLHTTGFRINVLAFKSCAPDYTTLSLEGDFGLVPSEYLQGVDFIPTKVKSWKSKNKAESNPTEVNSSVSNNKDDFIPTKVNKAAVGLFQKLSIDLIEQEKKYKNTPLIITGSGLGGYLAILSALRLHHAIDVEESNGSKNTKRPICITFGCPLVGDEALQRAIAERPQWKYSFLNVVANMDPIATFFSSDTPYKPFGTFLFCTESGGHTAFEDKDSIMLVLNAIKLSNEGNLQIYDYTNVLSSIRRKVLYRGVSELGDFNLNPLKAGITLQLREVGVLSTITNDEIGKMEKKQADKIKKKNLVSAYDPTKKLNEMKISLTYMEWYMKTRKSTGGYYDSYKNPKTKEELDRQNEIVRHHRVLNQYWKKFVEDKDRMPQKEGAKLRTRWLLGGNNYRRIVEPLEIAEYYRKGNKNYIETNRSDHFKLLEKWLDEDKDPDSSEVERNKAASLTEDSCFWAHVEEALILLSDVNRGSADIGQKLEKFEVDLMRAINNYSLSPDVFLDGSSLMKWWDEYKAHKGSGYASEFAEYMNKESYRSYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G8U5</accession>
    <name>A0AAD5G8U5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">TF-B3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_005588</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ARF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010402">
        <property type="protein sequence ID" value="KAI7731681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G8U5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009734">
        <property type="term" value="P:auxin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10017">
        <property type="entry name" value="B3_DNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.330.10:FF:000001">
        <property type="entry name" value="Auxin response factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.330.10">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044835">
        <property type="entry name" value="ARF_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003340">
        <property type="entry name" value="B3_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015300">
        <property type="entry name" value="DNA-bd_pseudobarrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31384:SF79">
        <property type="entry name" value="AUXIN RESPONSE FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31384">
        <property type="entry name" value="AUXIN RESPONSE FACTOR 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02362">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01019">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101936">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50863">
        <property type="entry name" value="B3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0927">Auxin signaling pathway</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TF-B3" evidence="8">
        <location>
            <begin position="62"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7731681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="168" mass="19156" checksum="6962037F5E056355" modified="2024-05-29" version="1">MPVCNLPAKILCHVYNVQLKAELDTDEVFAQITLMPEPNQDENAVKKEPQPPPESKFRVHSFWKTLTASDTSTHGGFSVFRRHADECLPPLDMSKQPPTQELVAKDLHENEWRFRHIFRGQPRRHLLQSGWSVFVSSKKLVAGDAFIFLSKYTCISEGTAKVRDVPRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GC60</accession>
    <name>A0AAD5GC60_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Flavonoid 3'-monooxygenase</fullName>
            <ecNumber evidence="17">1.14.14.82</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Flavonoid 3'-hydroxylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">M8C21_023706</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="23">AA19_3_7</strain>
            <tissue evidence="23">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Catalyzes the 3'-hydroxylation of the flavonoid B-ring to the 3',4'-hydroxylated state. Convert naringenin to eriodictyol and dihydrokaempferol to dihydroquercetin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a 3'-unsubstituted flavone + reduced [NADPH--hemoprotein reductase] + O2 = a 3'-hydroxyflavone + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16337"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:27741"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:138726"/>
            <dbReference type="EC" id="1.14.14.82"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 20">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Secondary metabolite biosynthesis; flavonoid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass type III membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 21">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.14.82" evidence="17"/>
    <dbReference type="EMBL" id="JAMZMK010009381">
        <property type="protein sequence ID" value="KAI7736089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GC60"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016711">
        <property type="term" value="F:flavonoid 3'-monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009813">
        <property type="term" value="P:flavonoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000056">
        <property type="entry name" value="Red aleurone1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47944">
        <property type="entry name" value="CYTOCHROME P450 98A9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47944:SF18">
        <property type="entry name" value="FLAVONOID 3'-MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0284">Flavonoid biosynthesis</keyword>
    <keyword evidence="5 20 21" id="KW-0349">Heme</keyword>
    <keyword evidence="11 20 21" id="KW-0408">Iron</keyword>
    <keyword evidence="13 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 20 21" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 21" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="8" id="KW-0521">NADP</keyword>
    <keyword evidence="10 21" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 22" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="20">
        <location>
            <position position="446"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00052910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00055665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00066562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00070032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00081480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="KAI7736089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="512" mass="56490" checksum="664135FE74434B27" modified="2024-05-29" version="1">MTILPILIYTTITTLIIYLLLNLRTRHHNRLPPGPTPWPIVGNLPHLGTIPHHSLAALASKHGPLMHLRLGFVDVVVAASASVAAQFLKAHDANFASRPPNSGAKHIAYNYQDLVFAPYGPRWRMLRKICSMHLFSAKALDDFRHVRQEEVAILTRALASAGKSPVKLGQLLNVCTTNALARVMLGRRVFGDGTGGGDRKADEFKDMVVEMMVLAGEFNIGDFIPALDWLDLQGIAKKMKKLHARFDSFLNTILEEHKSGNGGGSGHVDLLSTLIALKEDADGEGGKLSDIEIKALLLNLFVAGTDTSSSTVEWAVAELIRHPRLLKQAQEEMDKVVGRDRLVTESDLGQLTFLQAFVKENFRLHPSTPLSLPRIASESCEIDGYYIPKGSTLLVNVWAIARDPKMWTDPLEFRPTRFLPGGEKPNVDVKGNDFEVIPFGAGRRICVGISLGLRMVQLLVATLVQTFNWELANGLNPEKLNMNEAYGLTLQRAEPLMVHPKPRLAPHVYESG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-02-05" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GCM7</accession>
    <name>A0AAD5GCM7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PPM-type phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_004876</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009027">
        <property type="protein sequence ID" value="KAI7737425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039123">
        <property type="entry name" value="PPTC7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12320">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12320:SF1">
        <property type="entry name" value="PROTEIN PHOSPHATASE PTC7 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00331">
        <property type="entry name" value="PP2C_SIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="2">
        <location>
            <begin position="1101"/>
            <end position="1337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="155"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="234"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="549"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="675"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="155"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="244"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="550"/>
            <end position="561"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7737425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="1346" mass="146672" checksum="3EC82ED8CC4EF6E5" modified="2024-05-29" version="1">MSNTCSNLCNHKSFSFSTPLNPYTTTRFPLFPIQHSFLFIGRRSRGRSSSVPLKVAAQSSSSDLVLIATQEHHDGSVVFRFGDASEVVEKDDAVEDVESQTEQESSVVKVLDGDQDTQVIIKTGDSGYDSVEGRLVEVADGIRNADIGGDIAVSETNGIRDSSDNNAESSDTLSDVSEKEVLVSQPDANLSDFNDVVESTLDEVKFSVEPSISVELECTQVSKTEVVDYLDNVETEDASKSDTESASSELESMQVLQTEDRKSDTEDISPSAFPTTMAISDIELTDAANEDVEEDSKDMIVNEGTLIVEPPPSNPLDVQSMDAVNEDVEEDLKDMIVNEGTLIDELPLYNPLNVQLMDAVNEDAEEESQDMNINVATIIDEISSDQLIVEQPTLDEVSQLQAVDVKDQTTKDGDAEEELQDMNINEATIIDEMSSSDQLVVEQPTLDEVTQLQSVGVKDQTIEDELQQASTVNREENEVAYVMPVSRIEEEVTQPQSTPLDLQGILGQDRNNEIIQVDNETKYPSVSINLEAIQVSDNEVMDVTQPYAEDESRMVESKDTSEGDTQDLLPPATMHVSDVDDINQGLQDVNISEGAFTNEMPSSGPLGSEPPVEVLTTKDMDVKEVDMDERLQNMNVSEGNFIDEITSDPLGVEPTSEVSTAKYLDVKDVNTDERLQDTNTSEGTLINEMPTSDPIGAQPASEVSTTKDIDVKDIVIDERLQDTNASEGNFIDEMPTSDPLGVEPALEVSTANYTDVKDVDTDERLQDTNISEDTLIDEMPTSDLLGAEPTFEISVTEDMDVKDVDADERWQDMNTSEGTFGNDMPTSDPLGAERSLEVSTIKDMDVKDVDMNEMLKDNNIIEGTFIDEITTSDPLGAEPTLEVSTTEDVDVNDVDTEELLQDMNVGEGTFVDEMPTSDPVGAEPTLEVSTTVEAELQLVSNDNMDENLNAEAILEEPIVEEEASQPHLTPVELKATMKDSDIKLQDGTVDDEILQVIGEDADDSYIEDVEESSHKIESQPLQDEGVNHDMSEQCAETDAPESSILLKQGAHDPLLEAERTEGVVGSSELAEASEVTALLLAAEAEVEREEIYLTDDILLSGAALLEHPFKALTGGHDAYFVADGKWLGVANGVSQWSLEGTGLGVYAQELMRTCEEILLDTSNVPLTNPVELLCRGVKETNMSGSANVLIANFNGQALHVANIGDTGFLIIRHGAVYKKSSPLLHEFHFAVQVDDSDDPLQLVEEHLIELEVGDIVVSATDGLFDNLYEREITMIVSKSLQAGMKPQEIANILAKRAQEVGKSSFVRSPFSDAAQAAGYTGYAGGKPDNVAVIVSLVEKRSNLLAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GD53</accession>
    <name>A0AAD5GD53_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_032169</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008927">
        <property type="protein sequence ID" value="KAI7737905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GD53"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24296">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7737905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="374" mass="43174" checksum="406DC54F869B1015" modified="2024-05-29" version="1" fragment="single">MAFSINFLLTSILIPLFLLLIFLYIYNQSQTKKKYHPIGGTVFNMLINFKRLHHYMTDLAAKYKTFRMISPLHGEIYTTDPAIVEYILKTNFENYGKGKYTHEWLGDFLGDGIFTVDGDKWREQRKLSSHEFSTKVLRDFSSVTFRKNAIKVGNIFSAAANSNQVVDINDLFMKATADSIFKVAFGIDLDNLSGTNVEGVRFSRAFDDSNELSYNRLVDITWKMKKYLNIGSEAELKKNLKVVDEFVYKVIRIKSEQMSQPKDNSSITKDDILSRFMQMENRDPKYFRDIIMNYVLAGKDPIAATMTWFIYMLCKHPQIQDKVAKDIIQATGGMKEEITNVADFEARVNEEALEKMQYLQATLAETIRLYPALP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GEW7</accession>
    <name>A0AAD5GEW7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_024274</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the amino acid-polyamine-organocation (APC) superfamily. Polyamine:cation symporter (PHS) (TC 2.A.3.12) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008565">
        <property type="protein sequence ID" value="KAI7739810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GEW7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015203">
        <property type="term" value="F:polyamine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000041">
        <property type="entry name" value="Amino acid permease, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044566">
        <property type="entry name" value="RMV1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45826:SF2">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45826">
        <property type="entry name" value="POLYAMINE TRANSPORTER PUT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0769">Symport</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="32"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="56"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="92"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="168"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="244"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="341"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="364"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="396"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="425"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7739810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="475" mass="52526" checksum="91ECDC032A78804F" modified="2024-05-29" version="1">MGQHDDVQYVGINEPPSPTGATANNNMKGLSIVPLIFLIFYEVSGGPFGVEDSVQAAGPLLALIGFLVFPFIWSVPEALITAEMGTMFPEDGGYVVWVSSALGPFWGFQQGWMKWLSGVIDNALYPVLFLDYLKSGIPALADGYPRVLAVLALTVALTYMNFRGLTIVGWVAVLLGIFSLIPFVIMGLISIPKIEPARWLVVDLHVVDWNLYLNTLFWNLNYWDSISTLAGEVDNPRKTLPKALLYALILVVSGYFFPLLMGTGAVPLHRDQWTDGYFSDVAKIVGGVWLRWWIQAGAAMSNMGMFVTEMSSDSFQLLGMAERGMIPEFFAKRSRHGTPSIGILFSASGVILLSWLSFQEIVAAENFLYCFGMILEFIAFVRLRMKYPAASRPYKIPVGTVGSILLCVPPTILICVVLALSSLKVMIVSVIAIVIGFLLHPFLKLVEKKRWLKFSTKADLPDLLNTHENSESLVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GFB3</accession>
    <name>A0AAD5GFB3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tubby-like F-box protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_030176</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the TUB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008461">
        <property type="protein sequence ID" value="KAI7740322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GFB3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd22153">
        <property type="entry name" value="F-box_AtTLP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.50:FF:000047">
        <property type="entry name" value="Tubby-like F-box protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.90.10:FF:000003">
        <property type="entry name" value="Tubby-like F-box protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.90.10">
        <property type="entry name" value="Tubby Protein, Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025659">
        <property type="entry name" value="Tubby-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000007">
        <property type="entry name" value="Tubby_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018066">
        <property type="entry name" value="Tubby_C_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16517:SF127">
        <property type="entry name" value="TUBBY-LIKE F-BOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16517">
        <property type="entry name" value="TUBBY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01167">
        <property type="entry name" value="Tub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01573">
        <property type="entry name" value="SUPERTUBBY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54518">
        <property type="entry name" value="Tubby C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01200">
        <property type="entry name" value="TUB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01201">
        <property type="entry name" value="TUB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="4">
        <location>
            <begin position="184"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="Tubby C-terminal" evidence="3">
        <location>
            <begin position="245"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="573"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU361125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7740322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="573" mass="64093" checksum="9D0DC50588BFA2B0" modified="2024-05-29" version="1" fragment="single">KRGLPHAHICLFLNAESKFPTPSNIDRVISVEIPDKETDLYRRRQISNTVEKKGVQLDNSNNMIPLKHQPLLVLFSKKLLGRQFHKHVLIRLYAYVAVESPQRNFAYKLTRLLRGFKLIIIRLRGKNKNFGLIVRDVRDGFGSLSRRSFDVSRLTGHHSRGKSQGSVNDLSYIHAPLVIQSSRWANLPPELLCDVIKRLEESESTWPERKHVVACAAVCKSWRSMCKEIVGTPESCGKLTFPVSLKQPGPRDSTIQCYIKRDKSNLTYHLFLCLNPAALLDESGKFLLSAKRTRRTTCTEYVISMDADNISKSNGTYVGKLRSNFLGTKFIIYDTQPPHSGPLIPAPGRSSRRFYSKKVSPKVPNGSYSIAHITYELNVLGTRGPRKMHCIMHSIPASALEPGGFVPGQPELVLPPLSSLEDSFRSISFSKSLDRSTEFSSSRFSEIVGAAISNDVPESEKSKMPLVLKNKAPRWHEQLQCWCLNFRGRVTIASVKNFQLIAAQQPTAAAAAAPPPPSSAGTSQPTQVELDKVILQFGKVGKDMFTMDYRYPLSAFQAFAICLSSFDTKLACE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GFE5</accession>
    <name>A0AAD5GFE5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">Pectinesterase</fullName>
            <ecNumber evidence="4 10">3.1.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_010042</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 10">
            <text>[(1-&gt;4)-alpha-D-galacturonosyl methyl ester](n) + n H2O = [(1-&gt;4)-alpha-D-galacturonosyl](n) + n methanol + n H(+)</text>
            <dbReference type="Rhea" id="RHEA:22380"/>
            <dbReference type="Rhea" id="RHEA-COMP:14570"/>
            <dbReference type="Rhea" id="RHEA-COMP:14573"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17790"/>
            <dbReference type="ChEBI" id="CHEBI:140522"/>
            <dbReference type="ChEBI" id="CHEBI:140523"/>
            <dbReference type="EC" id="3.1.1.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the pectinesterase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the PMEI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.11" evidence="4 10"/>
    <dbReference type="EMBL" id="JAMZMK010008431">
        <property type="protein sequence ID" value="KAI7740427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GFE5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004857">
        <property type="term" value="F:enzyme inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030599">
        <property type="term" value="F:pectinesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042545">
        <property type="term" value="P:cell wall modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045490">
        <property type="term" value="P:pectin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd15798">
        <property type="entry name" value="PMEI-like_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.10:FF:000001">
        <property type="entry name" value="Pectinesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.40">
        <property type="entry name" value="Invertase/pectin methylesterase inhibitor family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035513">
        <property type="entry name" value="Invertase/methylesterase_inhib"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033131">
        <property type="entry name" value="Pectinesterase_Asp_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000070">
        <property type="entry name" value="Pectinesterase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006501">
        <property type="entry name" value="Pectinesterase_inhib_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01614">
        <property type="entry name" value="PME_inhib"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31707">
        <property type="entry name" value="PECTINESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01095">
        <property type="entry name" value="Pectinesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04043">
        <property type="entry name" value="PMEI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00856">
        <property type="entry name" value="PMEI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101148">
        <property type="entry name" value="Plant invertase/pectin methylesterase inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00503">
        <property type="entry name" value="PECTINESTERASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0063">Aspartyl esterase</keyword>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="5 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="29"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="domain" description="Pectinesterase inhibitor" evidence="12">
        <location>
            <begin position="66"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7740427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="551" mass="60860" checksum="F01A6D198F366020" modified="2024-05-29" version="1">MNSSLNSYGKVDEADQMRLDARRKTRKRLAIVAVSSLILVIVVVAAVVGTTRNKNHNHNDVSQSQSALASIKAVCDTTLYPDTCYNSLSSRVNSTHIKPDELLKLSIEVAINELSKASKLPELKNCSELFDLALDHLNDTLSSATNVDLGSLGSMLDDLMTWLSAAGTYQQTCIDSFEQNVLDNLKTSTELTSNCLAIIKGFSSVTTSYNQRRRLMSVEGSDDMPEWLSRKDRRLLQKASGIKADVVVAKDGTGKYKRIGDALKAVPDKSKKRFVIYVKKGVYIENVRVEKPKWNVMMIGDGMDSTIVSGSLNVVDGTPTFQSASFAVFGKGFIARDMGFRNTAGAAKHQAVALMSTADQSTFYRCRIDAFQDSLYAHSNRQFYRECNIYGTVDFIFGNSAVVLQNCNIHPRRPMTGQQNTITAQGKFDPNQNTGISIQNCNISPFGNLSDIHTFLGRPWKNHSTTVYMNNNMAGLIDPKGWMPWVGTSAPDTIFYAEFGNYGPGAVTKNRVKWKGLKFITSKQASKFTVKPFIQGNKWVKDAGVPYKSGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GHT9</accession>
    <name>A0AAD5GHT9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_030204</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008348">
        <property type="protein sequence ID" value="KAI7740786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GHT9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008278">
        <property type="term" value="C:cohesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990414">
        <property type="term" value="P:replication-born double-strand break repair via sister chromatid exchange"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007062">
        <property type="term" value="P:sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21793">
        <property type="entry name" value="Rad21_Rec8_M_AtSYN1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039781">
        <property type="entry name" value="Rad21/Rec8-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12585">
        <property type="entry name" value="SCC1 / RAD21 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12585:SF55">
        <property type="entry name" value="SISTER CHROMATID COHESION 1 PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="63"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7740786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="366" mass="39258" checksum="342E63D86E093E9D" modified="2024-05-29" version="1" fragment="single">PHPQTPPSTIDGVQDPGPDVNEDEVAPPETLRIVSPSFQKSPLGSFVLPDRVEPDEALVNEIEFNHNNNNTDDFSNINDIADNGGPSSPPKQISPQESMHSEHDHVQDSVGPHVSFELAPSSPRGKRPAEPAPAPPRVKRRRIKYDQATVLTNEFMKKSIDDASGIKRKRKGVSSFLDVYRINIAHKKEKVLSDPVITGLCDNLSELFEDDNVSSKAQLINIEQANPDAMEIGRFSPSIDRNDNMIPPISSPDNTIFSSPRPGDQDTPAFGTDIGSKSYQAATVGTSVGATSVGTSVGATSVGTSAGATSMRTTPNPASSIGSFMSDIETPFPQSHHGFDNSGGLYDIPEVDDAEEFAFIEVDGDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GHV7</accession>
    <name>A0AAD5GHV7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_030867</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007762">
        <property type="protein sequence ID" value="KAI7743260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GHV7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7743260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="126" mass="13690" checksum="4D54E777D69BD98D" modified="2024-05-29" version="1" fragment="single">FFNTLFSESSPSPTSTLTNPFEKTQKDPTGNPDKKLDPEGIALALVETRPGQPTGQPNTLSRKVLFASNLKIQIPDTCGDYGIKTRSPHFSGGSVGTGFGSPRSLTAVTRLFAVKNADVKKWFWMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GI83</accession>
    <name>A0AAD5GI83_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_000776</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007713">
        <property type="protein sequence ID" value="KAI7743577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GI83"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7743577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="48" mass="5439" checksum="4ECE8D9CC6B5B1A1" modified="2024-05-29" version="1">MVSTNAHKNLISEEKTNSEDLYANMATLSKSEMELITFGHGSAWRHEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GJC5</accession>
    <name>A0AAD5GJC5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_022605</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the plant CAR protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007422">
        <property type="protein sequence ID" value="KAI7744882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GJC5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009738">
        <property type="term" value="P:abscisic acid-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04038">
        <property type="entry name" value="C2_ArfGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044562">
        <property type="entry name" value="CAR1-11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45933">
        <property type="entry name" value="PROTEIN C2-DOMAIN ABA-RELATED 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45933:SF12">
        <property type="entry name" value="PROTEIN C2-DOMAIN ABA-RELATED 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0938">Abscisic acid signaling pathway</keyword>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="8" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="12">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7744882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="168" mass="18871" checksum="BD5431BD7E2AC740" modified="2024-05-29" version="1">MEGVVGLWKLRVKRGINLVARDTKSSDPYIVATLDDQKTKTKVSKGNCNPVWNDELTLAMRDPEAPIHIAVYDKDTFSNDDSMGEAEIDIKPYIECLRMGLSANNVPNGTELKRVHPTEHNCLANESSIFWENGKIVQDMNLKLRGVERGMVEIQIELVILPGHNLDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GK19</accession>
    <name>A0AAD5GK19_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glycosyltransferase</fullName>
            <ecNumber evidence="2">2.4.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_031265</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the glycosyltransferase 77 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.-" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010007966">
        <property type="protein sequence ID" value="KAI7742528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GK19"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044821">
        <property type="entry name" value="At1g28695/At4g15970-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005069">
        <property type="entry name" value="Nucl-diP-sugar_transferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46038">
        <property type="entry name" value="EXPRESSED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46038:SF13">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03407">
        <property type="entry name" value="Nucleotid_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="2" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="53"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleotide-diphospho-sugar transferase" evidence="4">
        <location>
            <begin position="145"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="16"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU363055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7742528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="396" mass="45642" checksum="1E9EF00B471091E9" modified="2024-05-29" version="1">MKQRNLRPYRTMGLECGNNKIVAGNPDERSPSPSSPPPPEFSLNQLFKQTPRMVIIFAVVALSCLVLYHTSFTYPSSRDYNSFAIRTRLANTNINPNELKTVLEKATMSNNTVIITTLNDAWAEPNSMFDLLLESFRIGNQTKRFLKHLVVVTLDQKAYARCLKLHPHCYRLSTDGTDFSEEAYFMAPDYLKMMWRRIDFLRSVLDLGYSFVFTDADIMWFRDPFPQFHKDADFQIACDYFNGNPSDLQNLPNGGFNYVKSNNKTIEFYKFWYNSRLTYPSLHDQDVFNKIKFNPFITNVGLRIRFLDTAYFGGFCQPSKDLNKVCTMHANCCVGLENKVHDLGLMLGDWRKYQKSLANQTSTQGFAGSWTVPQLCRGSFHRPRAPKKKDGQGRKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GKB5</accession>
    <name>A0AAD5GKB5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">CMP-sialic acid transporter 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_025831</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007745">
        <property type="protein sequence ID" value="KAI7743321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GKB5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015165">
        <property type="term" value="F:pyrimidine nucleotide-sugar transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007271">
        <property type="entry name" value="Nuc_sug_transpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10231">
        <property type="entry name" value="NUCLEOTIDE-SUGAR TRANSMEMBRANE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04142">
        <property type="entry name" value="Nuc_sug_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="50"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="84"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="129"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="160"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="194"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="225"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="254"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="280"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7743321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="310" mass="33530" checksum="4831BF309B15C72F" modified="2024-05-29" version="1">MASTKSKPSTTKNQSQNTAVNKKVWFYSLLLTIQYGAQPLISKRCIGRDVIVTSSVLACEIVKVICALVLMAKDGSLNKALKEWTLIGSLSASGLPAAIYALQNSLLQISYRNLDSLTFSMQKQSIQQIGALFILILAAVLLSIGEGSRKDYGIDNPDEIVLYGIIPVLIASVLSGLASALCQWASQVKKHSSYLMTVEMSLVGSLCLLASTYKSPDGEAMRQHGFFYGWTPLTMIPVFINAVGGILVGLVTSYAGGGFVIVSALLVTALLQFVFDGKPPSLFCLVALPLVMTSISIYQKYPYHIKKKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GLA0</accession>
    <name>A0AAD5GLA0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Bulb-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_014964</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in sporophytic self-incompatibility system (the inability of flowering plants to achieve self-fertilization).</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007699">
        <property type="protein sequence ID" value="KAI7743698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GLA0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048544">
        <property type="term" value="P:recognition of pollen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00028">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.90.10.10">
        <property type="entry name" value="Bulb-type lectin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001480">
        <property type="entry name" value="Bulb-type_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036426">
        <property type="entry name" value="Bulb-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000858">
        <property type="entry name" value="S_locus_glycoprot_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035446">
        <property type="entry name" value="SLSG/EP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32444">
        <property type="entry name" value="BULB-TYPE LECTIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32444:SF10">
        <property type="entry name" value="CURCULIN-LIKE (MANNOSE-BINDING) LECTIN FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01453">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00954">
        <property type="entry name" value="S_locus_glycop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002686">
        <property type="entry name" value="SLG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00108">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51110">
        <property type="entry name" value="alpha-D-mannose-specific plant lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50927">
        <property type="entry name" value="BULB_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042276078" description="Bulb-type lectin domain-containing protein" evidence="5">
        <location>
            <begin position="27"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="domain" description="Bulb-type lectin" evidence="6">
        <location>
            <begin position="33"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7743698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="446" mass="50822" checksum="FB1D4A685FDCA734" modified="2024-05-29" version="1">MASHPFIYVMPLLFLCTHILSTISRASVPSSETFTYVNSGELSETLVYIVEYDATYRDLPPYRNPFQLGFYNTTPDEYTLALRMGTVRSGSLRRWVWEANRGNPVHENATLTLRKDGNLVLADADGRVAWQTNTANKGVVRFQLLPTGNMVLQDAKGNFVWQSFDSTTDTLLVGQTLRANGPNKLVSRLSDLSNVDGPYSLVMEPKGLAMYYKSPNAPRPMKYWSSIERFTIDKGSMTNLTLNSEPDPDDGYLHYLSFDYFTTNPSSNQSSYIAYRKFDSRLSFLRLEIDGNLRLYTYDDNLDGGWVAWELVYTFLDRDSREGECQLPKRCGEFGLCEDNQCVACPTANGLSGWSKNCEVKKVTSCKASDFHYYMLQGVDHFMIKYTKGEGPMKQRDCVAKCTKDCKCMGYFYHTDEMRCWIAYDLKTLTRVGNLTHLAYIKTPNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GM89</accession>
    <name>A0AAD5GM89_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_024659</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007046">
        <property type="protein sequence ID" value="KAI7746094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GM89"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009506">
        <property type="term" value="C:plasmodesma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004714">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045272">
        <property type="entry name" value="ANXUR1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025886">
        <property type="entry name" value="PP2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27003">
        <property type="entry name" value="OS07G0166700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27003:SF471">
        <property type="entry name" value="VASCULAR ENDOTHELIAL GROWTH FACTOR RECEPTOR 2 (VEGFR2)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14299">
        <property type="entry name" value="PP2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7746094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="706" mass="81329" checksum="B7A06BCED50FB8B7" modified="2024-05-29" version="1">MLDSYGAMSSRNIYNVSSLQFTKETHKDNLLISFKDIKLATQNFNNLTRKGGFGLTYRGEILRANVSTPIAVKRYEHHQGYCNENNEKILVYEYASNGRLDRHLNNASLTWTQRLKIGIDVAIALDFLHGGASPVVHRDIKSGNILLHSHWKAKIADFGLSVIVPLNNEIDFVVDNACGTPGYIDPQYRERGFLTRKSDIYSLGVVLFEMMCGRLAFINASQDERKFLATLVKSHYGKGKVDELVFEGIKDKIVPESLTTFQRIAYQCLSYTREDRPTSSEVILQLKKALEFQEDIEIWEAKLPVDYREIIQMSKTPKKYSNVRNKYLYEMFSKGILLQKGKVFFSLSSNGERNEMVSATMFSYENNISHKRKTIQNSRFQRVVKIMDILNLKIHIKIKTQFLSPNVIYGAHLVFKFCDPRKISSKLMYVNLKYQMGSETLHAYFATCGEDEWMMIELCRFTPHKKDVGFEVLLESMSRYYCGSGGIYVEGIHFRAINDETLEGVQRVLESNSDSLQQASVDYDEITQIEDGEKLFSLSKPNDKKCHMLPAKMVLGDTSDVNCFNWKSLAEPESRFLEVAEYLSEQVFRIKCKIETQKLSVDTDYACYLIFKLSQECHGLQCPVKVQDVLLRKNKEFKFLYFRSPRLVNIHASGRVPKQREDGLMEVIVWEFNSGNKDYVPMSLKLRCYEGTMSGLIVYGIEFRPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GMF7</accession>
    <name>A0AAD5GMF7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Hexosyltransferase</fullName>
            <ecNumber evidence="5">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_000369</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1 5">Glycan metabolism; pectin biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Golgi apparatus membrane</location>
            <topology evidence="5">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the glycosyltransferase 8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="5"/>
    <dbReference type="EMBL" id="JAMZMK010006571">
        <property type="protein sequence ID" value="KAI7748255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GMF7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047262">
        <property type="term" value="F:polygalacturonate 4-alpha-galacturonosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029993">
        <property type="entry name" value="GAUT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002495">
        <property type="entry name" value="Glyco_trans_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32116">
        <property type="entry name" value="GALACTURONOSYLTRANSFERASE 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32116:SF31">
        <property type="entry name" value="GALACTURONOSYLTRANSFERASE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01501">
        <property type="entry name" value="Glyco_transf_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="3 5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7748255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="473" mass="54994" checksum="19FCDEAC115A81E1" modified="2024-05-29" version="1">MAISATLRRSRTTIPSLNTSNFPFRLFISILSVTLIFFFFLFTSSSHTDEGFSFSNNNININNIATSSRRTTLALKSDPLRPRFDQIRKQADDHRSLALAYAAYARKLKLENSKLVRVFADLSRNYTDLLSKPSYKALFESDSNSIDESTLRLFEKEVKERIKVTRQVIAEAKESFDNQLKIQKLKDSIFSVNEQLTKAKKQGAFSSLIAAKSIPKSLHCLTMRLMEERIAHPDKYSDEGKARPVEFDDPKLYHYAIFSDNVIAASVVVNSAVKNTKEPWKHVFHVVTDKMNLGAMQVMFKMKEYNGAHIEIKAVEDYKFLNSSYVPVLRQLESANLQRFYFENKLENATKDTTNMKFRNPKYLSILNHLRFYLPEMYPTLHRVLFLDDDIIVQKDLTGLWKIDMDGKVNGAVETCFGSFHRYSQYMNFSHPLIKEKFNPKACAWAYGMNFFDLDAWRREKCTEEYHYWQNLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GML1</accession>
    <name>A0AAD5GML1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_024110</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006510">
        <property type="protein sequence ID" value="KAI7748542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GML1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053303">
        <property type="entry name" value="Chloroplast_PPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47935">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN MRL1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47935:SF1">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN MRL1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12854">
        <property type="entry name" value="PPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7748542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="68" mass="7313" checksum="FDAF2F08DA564E64" modified="2024-05-29" version="1" fragment="single">MGFIQVCRSQKAVKEAFCFVSLIPIPILSTHNVFHEMVNFGVEPSIHTYGALIDGCAKAGQVSKAFGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GPX2</accession>
    <name>A0AAD5GPX2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Non-specific serine/threonine protein kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">M8C21_029618</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">AA19_3_7</strain>
            <tissue evidence="21">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006436">
        <property type="protein sequence ID" value="KAI7748929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GPX2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00028">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000330">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase At4g03230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001019">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase B120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.90.10.10">
        <property type="entry name" value="Bulb-type lectin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.110.10">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001480">
        <property type="entry name" value="Bulb-type_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036426">
        <property type="entry name" value="Bulb-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037176">
        <property type="entry name" value="Osmotin/thaumatin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001938">
        <property type="entry name" value="Thaumatin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002">
        <property type="entry name" value="RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE SD1-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002:SF548">
        <property type="entry name" value="RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01453">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08276">
        <property type="entry name" value="PAN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00314">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00347">
        <property type="entry name" value="THAUMATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00108">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00205">
        <property type="entry name" value="THN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51110">
        <property type="entry name" value="alpha-D-mannose-specific plant lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49870">
        <property type="entry name" value="Osmotin, thaumatin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50927">
        <property type="entry name" value="BULB_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50948">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51367">
        <property type="entry name" value="THAUMATIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0430">Lectin</keyword>
    <keyword evidence="12 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6 17" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042210763" description="Non-specific serine/threonine protein kinase" evidence="17">
        <location>
            <begin position="29"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="619"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="domain" description="Bulb-type lectin" evidence="19">
        <location>
            <begin position="242"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="20">
        <location>
            <begin position="512"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="18">
        <location>
            <begin position="695"/>
            <end position="860"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAI7748929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="23">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="860" mass="96599" checksum="EECABE1329AFFD5B" modified="2024-05-29" version="1">MKQNQLAFIQRFLFFVFFFFFFPNGVSGSIITVVNECGFTIWPAISGSPDLNITGFKLTEGNSRSFETPENWSGRLWGRTDCSFNGSSGHGSCKIADCGSGEMECNGRSPIPPVTIAEFDIKKSSYFVNLRDGYNLPITIEPAGGSVYSSLDCTRGCASDLNKRCPKELMLDGGGGCQSPCQVFGTPMYCCSKSLMTCEPTAYSRFFHAACPRSDDVTSPDLYTVCVSANYTIRFCPPEDAFSTLKVGSKLGPYDQLVSISGKFTLGFFNGLRNYLAIWYTGDVEARKVWVANPNTPVMPMSCALSIDPNTGNLIITNGGKTLVKITDVQAGRNPNVTATLEDDGNFRLINDMDKRVLWQSFDHPTNVLLPGMKLGYDTRTRQNWTLTSWLSNGSKAFTLSWEPINETSQRLMIRRRGQPYWTSGFLDVKTSAFMYLQNGDMQSKYNLTSVYNNEERYFSYVTDDMADAFPMWILTPKGQLSNSNTSNEWTPDLCYGHNSSNGCLNPSLPLCRREDDSFSHRKGEFTPQGTRNSTESNSGISISDCFVKCWNDCSCVGFNSSNTNGTGCVIWTGIRNNIFVDGGDNSTMKYVINFDLPDNSGTSDHDLGNKMRKNLKQIWALVVVPLFLGLGLLWYIKKRKQNRQEYKRRKRDEYFLELAASESFKDVHQLESDGRYGDLLLFSFASIMTATNDFSPENKLGQGGFGPVYKGTLNDGREIAIKRLSKSSGQGLVEFKNELMLIAKLQHTNLVRILGCCIHGEEKMLVYEYMPNKSLDFFLFDENKRAELNWPKRFSIIEGIAQGLLYLHKHSRMRVIHRDIKSNNILLDESMNPKISDFGMARIFNQNETQAMTNRVVGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GPZ9</accession>
    <name>A0AAD5GPZ9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ubiquinone biosynthesis protein COQ4 homolog, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">4-hydroxy-3-methoxy-5-polyprenylbenzoate decarboxylase</fullName>
            <ecNumber evidence="6">4.1.1.130</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Coenzyme Q biosynthesis protein 4 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_026664</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Lyase that catalyzes the C1-decarboxylation of 4-hydroxy-3-methoxy-5-(all-trans-polyprenyl)benzoic acid into 2-methoxy-6-(all-trans-polyprenyl)phenol during ubiquinone biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 4-hydroxy-3-methoxy-5-(all-trans-polyprenyl)benzoate + H(+) = a 2-methoxy-6-(all-trans-polyprenyl)phenol + CO2</text>
            <dbReference type="Rhea" id="RHEA:81179"/>
            <dbReference type="Rhea" id="RHEA-COMP:9551"/>
            <dbReference type="Rhea" id="RHEA-COMP:10931"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:62731"/>
            <dbReference type="ChEBI" id="CHEBI:84443"/>
            <dbReference type="EC" id="4.1.1.130"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="6">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of a multi-subunit COQ enzyme complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Mitochondrion inner membrane</location>
            <topology evidence="6">Peripheral membrane protein</topology>
            <orientation evidence="6">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the COQ4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.130" evidence="6"/>
    <dbReference type="EMBL" id="JAMZMK010006067">
        <property type="protein sequence ID" value="KAI7750880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GPZ9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031314">
        <property type="term" value="C:extrinsic component of mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120539">
        <property type="term" value="F:4-hydroxy-3-methoxy-5-polyprenylbenzoate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03111">
        <property type="entry name" value="Coq4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007715">
        <property type="entry name" value="Coq4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027540">
        <property type="entry name" value="Coq4_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12922">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12922:SF7">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS PROTEIN COQ4 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05019">
        <property type="entry name" value="Coq4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="2 6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="6">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7750880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="225" mass="25567" checksum="5764D6527A535FDD" modified="2024-05-29" version="1">MVEGARVRLRGWQQAAVALGSAVGALVDPRRADLIAALGETTGKPAFERVLERMKRNPEGREILLDRPRVISSNVGHAWDLPANTFGAAYAKFMGSRNFSPDDRPPVRFMETEELAYVAMRAREVHDFWHTLFGLPTNLIGESALKVIEFEQMLLPMCFLSVIGGTARFNDKQRSLFYKHYFPWAVQAGMKSTDLMCVYYEKHFHEDLEVVRRRWGIIPAPAVLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GQW3</accession>
    <name>A0AAD5GQW3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_012747</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005820">
        <property type="protein sequence ID" value="KAI7751780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GQW3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7751780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="65" mass="7440" checksum="3B7A00886ABCA1C1" modified="2024-05-29" version="1" fragment="single">EDNGSSGWLFTTLLLKWTQHSNQSNLIAYPTFEVVKPQFLVIFDSEGTCYDGEVGKCFYQLMVSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GRU3</accession>
    <name>A0AAD5GRU3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_026318</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006189">
        <property type="protein sequence ID" value="KAI7750221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GRU3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7750221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="72" mass="8463" checksum="0863E0369B1C0CAC" modified="2024-05-29" version="1" fragment="single">GVYKFWNNSCITMFDETVRTIEKRIADFTFLPVGVNRHFGMEVFTKGVEAQQVDGWLPRQADTFEKFHKECD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GSW3</accession>
    <name>A0AAD5GSW3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_007083</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005755">
        <property type="protein sequence ID" value="KAI7752109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GSW3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34451">
        <property type="entry name" value="PHD FINGER FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34451:SF7">
        <property type="entry name" value="PHD FINGER FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="277"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="291"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7752109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="300" mass="31822" checksum="B1823806D21966B1" modified="2024-05-29" version="1">MMKQDSIPTTPTLPTVCGQCGTDERTLLHNVRLRGNFRRLCTTCVLRLHPQCFCPACLGVYDRLPPDDAVVCYKCYSASHPSCVPSHSPVVGAIASSGRGPVPCSACLNPNLLVLNVGRVENGGRVGRVIDCGAARLLLAAGKIAAMSMGKAEVAAGMEAERRAKEAAYTKKRAREALDHVVRLMGREKRNVSVNVNVNVNGNGNGNVNNVVGNVVVSKDDDSNEVLKALNAVELKDNGGVVVDEKAVVMEVDVGGKESGAENGCVKSDEVVKIAQNGEEDQGVEKMQAVEENDVSENVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-02-05" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GSY8</accession>
    <name>A0AAD5GSY8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FCP1 homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_017091</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010004103">
        <property type="protein sequence ID" value="KAI7754227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050365">
        <property type="entry name" value="TIM50"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12210">
        <property type="entry name" value="DULLARD PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FCP1 homology" evidence="1">
        <location>
            <begin position="312"/>
            <end position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7754227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="897" mass="99005" checksum="A50DAE90D7FE6C9D" modified="2024-05-29" version="1">MATKDSITKNVVVASDHIPVTKEEGAETEATINNVIVSDNSHSNDKEDVTMMKKVVASDNTEEDDDDNEKETGSDLGVIMDKLNIGLKKKLVVLPINVYKRPFCDGFLKFCFERFEVGIWSSAMEHNIGAVLNNVMGELKSKLLFTWDQNQCTKTDFKCLDNKDKPVFLKELDHLWSKKYSINLPWCDGEYSSSNTQTPQFLLGIMIPTTRXXXXLGPNGEMRVFLEGLAEAKDVQSYVKAHPIGDPAITSSHADWDYYSKIIRALGKKERIQVKDDKKKTMIASSDPISKEKDDEKETGCDLGVSMDKLNLGPKKKLLVLPLGGFLVHRAHRRRPNTIPKNRSPDFSSGNFMIYKRPFCQEFLEFCFQRFEVGIWSTAMEHNISGVLTNVMGEHKSKLLFTWDQNQCTDTCFTYPDNPNKPLFVKELKHIWKKKYIYGPWSEGDYSAANTLLITYPVKALLNPPNTSIYPEDYDPENSEDDILGPNSELRAFLDGLAEAEDVQSYVKAHPIGDPAISSSHPDWDYYSKIIRAFVVASDDSRISKEVDAEKDAGTKNVVVVADDSCIYKEEDAGTMNAVVVSDDSRINKEDVGTMNAVVASDDSCVSKEDAAEKDAGTKNVVVVSDICVYKEEDAGTMNAVVVADDSCLIKEDVGTMNAVVVSDDSHINKEDAGTMNVVVASNDSCVSKEDDAEKDAGTKNVVVVSDICINKEEDAGTMNVVSDDSLVSKEGDAVKDAGTKNDVVFSDHNTVSKEDVETDAGTDNVVVASDHSPVSKENAVETEATINNVVASDNSGTSEKEDKEDDMMKKVVASDNTEDEDNGKETGSGLSILMGKLNLGPKTKLLEEIYSCTDVVSAMGQAVMETNVEVLGPNVVRRSRRVRKVPEKLKDYHVGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GTM3</accession>
    <name>A0AAD5GTM3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Carbonic anhydrase</fullName>
            <ecNumber evidence="3">4.2.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Carbonate dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_016761</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Reversible hydration of carbon dioxide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>hydrogencarbonate + H(+) = CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:10748"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="EC" id="4.2.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="2">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the beta-class carbonic anhydrase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.1" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010002479">
        <property type="protein sequence ID" value="KAI7754780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GTM3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004089">
        <property type="term" value="F:carbonate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1050.10">
        <property type="entry name" value="Carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001765">
        <property type="entry name" value="Carbonic_anhydrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036874">
        <property type="entry name" value="Carbonic_anhydrase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11002">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11002:SF12">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00484">
        <property type="entry name" value="Pro_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00947">
        <property type="entry name" value="Pro_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53056">
        <property type="entry name" value="beta-carbonic anhydrase, cab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="2">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR601765-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7754780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="216" mass="24171" checksum="0E6FC5748C6C2521" modified="2024-05-29" version="1">MSLRLQASADGVALVQETTETTPTLIKTTDDGNLPFTEMKNRFLSFKKHKYLENLECYQQLAEAQAPKNGPCETNAALEFSVTTLEVANILVTGHSCCGGIRALMGMEDEVNSSSFIKNWVIVGKAAKSTTKATASNLSFDQQCKHCEKESINNSLLNLLTYPWVEERVAKGLLSLHGGYYDFVNCTYEKWTLDYQRSNPKQETGGYAIKNREFWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GV51</accession>
    <name>A0AAD5GV51_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">glyceraldehyde-3-phosphate dehydrogenase (NADP(+)) (phosphorylating)</fullName>
            <ecNumber evidence="5">1.2.1.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_018492</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>D-glyceraldehyde 3-phosphate + phosphate + NADP(+) = (2R)-3-phospho-glyceroyl phosphate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10296"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57604"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="1.2.1.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate biosynthesis; Calvin cycle.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Tetramer of either four A chains (GAPDH 2) or two A and two B chains (GAPDH 1).</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.13" evidence="5"/>
    <dbReference type="EMBL" id="JAMZMK010000368">
        <property type="protein sequence ID" value="KAI7756475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GV51"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047100">
        <property type="term" value="F:glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019253">
        <property type="term" value="P:reductive pentose-phosphate cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000002">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020831">
        <property type="entry name" value="GlycerAld/Erythrose_P_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020829">
        <property type="entry name" value="GlycerAld_3-P_DH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020828">
        <property type="entry name" value="GlycerAld_3-P_DH_NAD(P)-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43148">
        <property type="entry name" value="GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02800">
        <property type="entry name" value="Gp_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00044">
        <property type="entry name" value="Gp_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00078">
        <property type="entry name" value="G3PDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00846">
        <property type="entry name" value="Gp_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0113">Calvin cycle</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyceraldehyde 3-phosphate dehydrogenase NAD(P) binding" evidence="9">
        <location>
            <begin position="97"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00039137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00052787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00063826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7756475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="378" mass="40442" checksum="1F191E6DBD6F2E60" modified="2024-05-29" version="1" fragment="single">MASNAALASTRIPSITRLPAKTSISTSFPSQCSIKRSEVAEFTGLRSSGSVTYAKYVKDSSFFDTVSSQFIPKDDAKLLTTAAAGSGVVKVQTVAKLKVAINGFGRIGRNFLRCWHGREDSPLEVVVVNDSGGVKNLDCGCELRYVQASHLLKYDSMLGTFKADVKIVDNETISVDGKLIKVVSSRDPLKLPWAELGIDIVIERLLDASHRDLRRARAAALNIVPTSTGAAKAVSLVLPQLKGKLNGIALRVPTPNVSVVDLVVNVAKKGLTAEDVNAAFRKAADGPLKGILSVCDEPLVSVDFRCSDVSSTIDSSLTMVMGDDMVKVVAWYDNEWGYSQRVVDLAHLVAAKWPGAAVAGSGDPLEDFCKKNPGEEEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GVP7</accession>
    <name>A0AAD5GVP7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">MYND-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_026278</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010004903">
        <property type="protein sequence ID" value="KAI7754016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GVP7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="6.10.140.2220:FF:000033">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057136">
        <property type="entry name" value="At2g35280_TPR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044508">
        <property type="entry name" value="At5g50450/At1g67340-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002893">
        <property type="entry name" value="Znf_MYND"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46758">
        <property type="entry name" value="MYND DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46758:SF2">
        <property type="entry name" value="OJ1485_B09.11 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23310">
        <property type="entry name" value="TPR_27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01753">
        <property type="entry name" value="zf-MYND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144232">
        <property type="entry name" value="HIT/MYND zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50865">
        <property type="entry name" value="ZF_MYND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MYND-type" evidence="6">
        <location>
            <begin position="352"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="19"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7754016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="424" mass="45641" checksum="B3865B2E03408EBC" modified="2024-05-29" version="1">MVVVCAAVAGGGGGWGRSGGGRFDRSASRQTKRTIEEEDGRTNGSGQLRLTTFLFLHILLTKQIRTNSCSSKGAKSSTGTPVVTVSGQCDFLDTLPDDIVLFILTKLGSTASRPADFISILSTCKRLNSLGVNSFVLSKASPKTFAVKAKNWSESSHRFLKQCSDAGNVEACYTLGMIRFYCLQNRGSGASLMAKAAINSHAPSLYSLAVIQFNGSGGSKSDKDLRAGVALCARAAFLGHIDALRELGHCLQDGYGVRQNIAEGRRFLVQANARELAAVLSTTTESIVTGSWLTWNPLPHLRNGNVVGNGCPLLSDFGCNVPPPEPHPANQFLSDWFAGKELSGAGLRLCSHTGCGRPETRLHEFRRCSVCGKVNYCSRACQALDWKLRHKMECIQPERWVDVGDVNGDGNGNVNEDDVMFVES</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GVT5</accession>
    <name>A0AAD5GVT5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">MADS-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_014880</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010004440">
        <property type="protein sequence ID" value="KAI7754136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GVT5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00265">
        <property type="entry name" value="MADS_MEF2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1810.10">
        <property type="entry name" value="Transcription factor, MADS-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050142">
        <property type="entry name" value="MADS-box/MEF2_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033896">
        <property type="entry name" value="MEF2-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002100">
        <property type="entry name" value="TF_MADSbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036879">
        <property type="entry name" value="TF_MADSbox_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48019">
        <property type="entry name" value="SERUM RESPONSE FACTOR HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00319">
        <property type="entry name" value="SRF-TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00404">
        <property type="entry name" value="MADSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00432">
        <property type="entry name" value="MADS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55455">
        <property type="entry name" value="SRF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50066">
        <property type="entry name" value="MADS_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MADS-box" evidence="6">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7754136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="180" mass="21055" checksum="25A1655AE91DD0E0" modified="2024-05-29" version="1">MGRGKVELKRIEDKASRQVSFTKRRNGLMKKAHELSVLCDVDLAVFVFSGKNKLYEFSTGNSMPEILRRYEDHKHADEAVRKSVREELMSQYRDVRTADELTHMIQGHLNERKVQQANLTELCQLEEQTDTILQQVRTAKTQLMLGVVKNLQDEQKQLRQEKQLMERDLTNKLPIMDLNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BJR5</accession>
    <name>A0AAD5BJR5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycoside hydrolase family 3 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_016863</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012225">
        <property type="protein sequence ID" value="KAI7724412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BJR5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008422">
        <property type="term" value="F:beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009251">
        <property type="term" value="P:glucan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1700">
        <property type="entry name" value="Glycoside hydrolase family 3 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051915">
        <property type="entry name" value="Cellulose_Degrad_GH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002772">
        <property type="entry name" value="Glyco_hydro_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036881">
        <property type="entry name" value="Glyco_hydro_3_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30620:SF35">
        <property type="entry name" value="GLYCOSYL HYDROLASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30620">
        <property type="entry name" value="PERIPLASMIC BETA-GLUCOSIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01915">
        <property type="entry name" value="Glyco_hydro_3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52279">
        <property type="entry name" value="Beta-D-glucan exohydrolase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="148"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 3 C-terminal" evidence="4">
        <location>
            <begin position="13"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7724412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="173" mass="18830" checksum="90570488B24D9C7A" modified="2024-05-29" version="1">MENPNAEYIKSNNFSYAIVVVGEHPYTEMFGDSSNLTIADPGPSIITNVCENVKCVVVIISGRPVVIEPYISVIDALVAAWLPGTEGQGVSDVLFGDYGFTGKLSRTWFRTVDQLPMNIGDAHYDPLFPFGFGLTTKSMTERSISGGVAIRPFVLAILVSVFISLSLSRRYIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BKA4</accession>
    <name>A0AAD5BKA4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_031202</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012160">
        <property type="protein sequence ID" value="KAI7724777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BKA4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7724777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="47" mass="5433" checksum="B24E8DBA612B4B68" modified="2024-05-29" version="1">MHNSHLLTHDVPYFILNMFVKPRPVDDKSTQNATNLDNIHTTVIQSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BKJ9</accession>
    <name>A0AAD5BKJ9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">C2 and GRAM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_009126</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012108">
        <property type="protein sequence ID" value="KAI7724983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BKJ9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00030">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044511">
        <property type="entry name" value="At1g03370/At5g50170-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004182">
        <property type="entry name" value="GRAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031968">
        <property type="entry name" value="VASt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46296">
        <property type="entry name" value="BNAA05G37250D PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46296:SF8">
        <property type="entry name" value="OS06G0297800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02893">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16016">
        <property type="entry name" value="VASt"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00360">
        <property type="entry name" value="C2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00568">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51778">
        <property type="entry name" value="VAST"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="C2" evidence="6">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="VASt" evidence="7">
        <location>
            <begin position="251"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="6">
        <location>
            <begin position="524"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="domain" description="VASt" evidence="7">
        <location>
            <begin position="872"/>
            <end position="1039"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="130"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="823"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="143"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="158"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="179"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="204"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="823"/>
            <end position="835"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7724983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1040" mass="116488" checksum="709F08072244187A" modified="2024-05-29" version="1">MKLLVRVIEARNVPAMDQNGSSDPYVKLKLGNQRFKTKVVKKCLNPSWCEEFSFKVEDLKEQLVVSVFDEDKFFSDDFVGSVKIPVSSVFDTVDKSLGTVWYPLQPKKKSKIKDCGEILLTICFTQNNPQSDLQPQVENGAVSPSRSSTSSRMSSPLRSEELTAPVKEEKSNKEKITGLISQIFSRNNEPAPPLTSNKNTELPEVPETDESEVSEDKIEEQSSSSSSSNFEELMKKLEEKDDGGEMPANLPGGIVLDQMYAISPSELNSFIFSSESDYLKQLADIQGSTDLQMGPWKFDAETGSLKRTFTYVKAPSRLIKALKAIEDQTYLKANGKCFSVLASVNTPEAPYASNYRVEVQTNISPGPELPSGEECSHLVVSWRMNFIHNTMMKGMIEGGARQGVKESFEQVAGLLSEKLKVVDVKGIGSEKDQALASLQVEKQSDWKLAVQYFANFTVVSTVLMGLYVLVHIFLSMPSTIQGLEFGGLDLPDSIGEVVVCGVLVLQGERVMHLISRFMQARVHKGGDHGIKATGDGWQLTVALVEGCNIPTVESNVLSDPYVVFTCNGKTRTSSIKFQKSDPRWNEIFEFDAMDDPPSTLDVEVFDFDGPFDEAVSLGHTQINFVRSNITDLGDVWVPLQGQLAQACQSKLHLRIFLNNTRGSNIIKEYLSKMEKEVGKKIRVRSPQTNSAFQKLFKLPPEEFLINDFTCHLKRRMPLQGRLFLSARIIGFHGDLFGHKTNFYFLWEDIEDVQVLQPTLSSMGSPVVVITLHPGRGLDAKHGAKTQDAEGRLKFHFQSFVSFSVAHRTIMALWRARSLSPEQKAEIAEKESDDKNQQMLEDESSSIKSDDESDNRSLLSEESGSFLGLEDVSMSVVYSSVLSVPSNFVMELFSGSDLERRAMDRAHCVNYSTSPWEFEKTDVYQRQTYYKFDVSVSRYGGEVTSSQQKSRLTDRNGWLVEEVTTLHGVPLGDYFTLHTKYQIEDQGSRSTGCKVVVHFGIAWLKSTKHKKKITKNIHANLQDRMTILFSTIEKEFISGKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BLQ6</accession>
    <name>A0AAD5BLQ6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_005382</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011893">
        <property type="protein sequence ID" value="KAI7725625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BLQ6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34188">
        <property type="entry name" value="OS01G0299500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34188:SF5">
        <property type="entry name" value="OS05G0131900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="140"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7725625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="216" mass="23266" checksum="D9FFD00B4229E623" modified="2024-05-29" version="1">MDYSDSKAQGDGFDVDLESGVGIVQSNAERISDSDSDKKLAKTAFLRLCDGLMSVDDDSLVKSDTGNVNEGCLESIVKCKKGSSAKKPPRPPRPLSLDAYDQKLIKELAELAMIKRARVERMKALKQKKNSKISSSSSSSHGSVFAMIFTIIFFLVILFQGHNSGVTIQATPQTGQVKAAPQTGQRNTNGLTVLQNQLNISPSVSVSSNYKSSTGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BMM0</accession>
    <name>A0AAD5BMM0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_019161</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012076">
        <property type="protein sequence ID" value="KAI7725063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BMM0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7725063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="68" mass="7717" checksum="81FB945268D8C28B" modified="2024-05-29" version="1">MQGLYCNPRLAVVINHRSCLKTRAFSSDSSNQSRGGLPRFFSDGSGVRVQGDEFWHMTKVLRLNTDDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BMN7</accession>
    <name>A0AAD5BMN7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_015261</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012103">
        <property type="protein sequence ID" value="KAI7724999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BMN7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7724999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="86" mass="9744" checksum="317E9C19703ECA31" modified="2024-05-29" version="1">MATDEDDQGFGIQRKRKREVPRSYRCGGHTTEMATSDASSTSTQWSNNEDRRQSWPSGAGSCTHGQPRQTKQMRSVIPPNNWKFTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BNL8</accession>
    <name>A0AAD5BNL8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_008651</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="JAMZMK010011601">
        <property type="protein sequence ID" value="KAI7726707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BNL8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001023">
        <property type="entry name" value="Os07g0541700 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002:SF851">
        <property type="entry name" value="G-TYPE LECTIN S-RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE SD1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27002">
        <property type="entry name" value="RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE SD1-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="1"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7726707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="121" mass="14439" checksum="E26F4F687D2D1E42" modified="2024-05-29" version="1">MSSSQGYEEFRNEVIFISRLQHRNVVKILGYCFEGQEKMLVYEYMPNNGLDLFLFLKKSLADEQKSKTIDWSTRCHIIDGIARGLLYLRQDSRLWIIHRDLKPANILLDIRFWPCKKLWGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BNY5</accession>
    <name>A0AAD5BNY5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_003325</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011497">
        <property type="protein sequence ID" value="KAI7726877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BNY5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1050.10">
        <property type="entry name" value="At2g23090-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039713">
        <property type="entry name" value="At2g23090-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039438">
        <property type="entry name" value="At2g23090-like_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007513">
        <property type="entry name" value="SERF-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026939">
        <property type="entry name" value="ZNF706/At2g23090_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33788:SF20">
        <property type="entry name" value="GENOME ASSEMBLY, CHROMOSOME: A03"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33788">
        <property type="entry name" value="OS07G0114300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04419">
        <property type="entry name" value="SERF-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12907">
        <property type="entry name" value="zf-met2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118359">
        <property type="entry name" value="Expressed protein At2g23090/F21P24.15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Small EDRK-rich factor-like N-terminal" evidence="2">
        <location>
            <begin position="3"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="domain" description="At2g23090-like zinc-binding" evidence="3">
        <location>
            <begin position="39"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7726877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="78" mass="8583" checksum="1A1478B774615A33" modified="2024-05-29" version="1">MGGGNAQKSKMAREKNLEKARAAGKGSQLEANKKAMSIQCKVCMQTFICTTSEVKCREHAEAKHPKADVYACFPHLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BP27</accession>
    <name>A0AAD5BP27_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_033246</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011476">
        <property type="protein sequence ID" value="KAI7726947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BP27"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000032">
        <property type="entry name" value="Serine/threonine-protein kinase PBS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050823">
        <property type="entry name" value="Plant_Ser_Thr_Prot_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45621">
        <property type="entry name" value="OS01G0588500 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000654">
        <property type="entry name" value="Integrin-linked_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="1"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="203"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="212"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="241"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7726947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="250" mass="27832" checksum="54131E8B3D7691E6" modified="2024-05-29" version="1">MQRGSFENHLFGRGSTVQPLPWDIRLKIAIGAARGLAFLHTSDKKVIYRDFKASNILLDGSYNAKLSDFGLAKMASASQSHVTTRVMGTYGYAAPEYVTTGHLYVKSDVYGFGVVLVEMLTGMRTLDNGRPAAQRNLVDWVKPYLANRRKLKNVMDPRLEGRYPSKAAAQVAQLALTCLGTEQKTRPSMKEVVATLERLGDIKERPKVPRIHNSSPRSPYPNGQQPLRHRSPLHVKEDVSQRSQDSPKGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BPB0</accession>
    <name>A0AAD5BPB0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_002382</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011470">
        <property type="protein sequence ID" value="KAI7727010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BPB0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="121"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7727010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="143" mass="17105" checksum="30653B00B15C5800" modified="2024-05-29" version="1" fragment="single">MFSTNNVSYKTRSWVHCCCSVADCFLGSFHQFFKQVHQATCLSVRVLDNPMFLQFWELQMIINKMEVITEIVQNIRASPTIYIRRIKFRFPTIKRPFSRMLISQRDICNNMMKWSFLTLKWVSFLINLHSLINLTFATYFDLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BR87</accession>
    <name>A0AAD5BR87_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_025922</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011549">
        <property type="protein sequence ID" value="KAI7726739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BR87"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005876">
        <property type="term" value="C:spindle microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008574">
        <property type="term" value="F:plus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090307">
        <property type="term" value="P:mitotic spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051231">
        <property type="term" value="P:spindle elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047149">
        <property type="entry name" value="KIF11-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47970:SF12">
        <property type="entry name" value="KINESIN FAMILY MEMBER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47970">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIF11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0505">Motor protein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="161"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7726739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="206" mass="23101" checksum="E2418DF804C8C213" modified="2024-05-29" version="1">MDTTGVRTRMDTTRARTKACSCRAFKFISGADMDTAEEDKLSAGNRSVVNKFESELAQDVGSLCNMVATSVSQQNEQLQCIEKFCDTFINISDKAITDLKTKVSTSRNLYISHIEEMENVIRLRKASANGSLEDIQPAFCQMNISVVGHNRIFQCSLQLKSLWFAIYMIIHGHSVIYTVAQDDFKKVYQKGAIENHPSKGRDPNCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BTR1</accession>
    <name>A0AAD5BTR1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">M8C21_018535</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="25">AA19_3_7</strain>
            <tissue evidence="25">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19 27">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18 27">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="25">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010011021">
        <property type="protein sequence ID" value="KAI7729315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BTR1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000129">
        <property type="entry name" value="Leucine-rich repeat receptor-like kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000198">
        <property type="entry name" value="receptor protein kinase TMK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000226">
        <property type="entry name" value="receptor protein kinase TMK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000190">
        <property type="entry name" value="Receptor-like kinase TMK4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052422">
        <property type="entry name" value="Auxin_Ser/Thr_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47986">
        <property type="entry name" value="OSJNBA0070M12.3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47986:SF29">
        <property type="entry name" value="RECEPTOR PROTEIN KINASE TMK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="14 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-0675">Receptor</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8 23" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 22" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="23">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042054842" description="non-specific serine/threonine protein kinase" evidence="23">
        <location>
            <begin position="24"/>
            <end position="938"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="479"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="24">
        <location>
            <begin position="584"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="437"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="450"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="612"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="KAI7729315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="938" mass="101964" checksum="79F062393C3461A9" modified="2024-05-29" version="1">MITQVKLTIVILLSSILIHYVHSQTNSNDIQVMLSLKKSLNPPNLDWSDPNPCKWAHVSCSEDNRVTRIQIGHQNLQGTLPSTLSNLTQLERLELQWNNISGPLPGLSGLSSLQVLMISNNMFSSIPSDFFTGMSSLQSLELDNNPFAKWVIPESIKDASTLQNFSAVNANVIGTIPEFFGSDSFPGLVNLHLAINGLEGGLPMSLSGSQLESLWVNGNKLNGTIDVIQNMTSLKEIWLHSNAFSGSLPDFSGLKELEVLSVRDNAFTGLVPESLMSLESLKSVNLTNNILQGPMPKFKDSVSVDLVKGSNDFCLDEVGDCDPQVMTLLAIAKSMDYPAKFASNWKGNDPCVDWIGITCNNGNVTIINFQKMGLSGTISPAFSELKSLQRIVLANNNLTGTIPESLATLPALSELDLENNDLYGKVPVFKSNLKVNTNGNPDIGKDRSSSSDPNSKSPSGSPDSDGVSLNGKKSSKSRIGVVLFSILGVVLVMFLVGGLAFCLYKKKQEKVSGVQTPNAIVIHPCNSGSDNESVKITVAGSSVSVGGLTETHTVQANEGSDIQMVEAGNMVISIQVLKTVTNNFAEENILGQGGFGTVYKGELHDGTKIAVKRMECGVITGKGVAEFQSEIAVLTKVRHRNLVALLGYCLDGNEKLLVYEYMPQGTLSRHLFNWPEEGLSPLEWTRRLAIALDVARGVEYLHGLAQQSFIHRDLKPSNILLGDDMRAKVGDFGLVRLAPEGKGSVETRIAGTFGYLAPEYAVTGRVTTKVDVFSFGVILMELITGRKALDETQPEESMHLVTWFKRIHLNKDTFRKSIDQTLDLTEENLSSISKAAELAGHCCAREPYQRPDMSHAVNVLSSLVEMWKPSDQSSDDIYGIDLDLSLPQALKKWQAFEGGSRTMDASSSSFVPSLDNTQTSIPTRPYGFAESFTSMDGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BWV6</accession>
    <name>A0AAD5BWV6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Rhodanese domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_031297</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010878">
        <property type="protein sequence ID" value="KAI7729938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BWV6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070566">
        <property type="term" value="F:adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004792">
        <property type="term" value="F:thiosulfate-cyanide sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042292">
        <property type="term" value="F:URM1 activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002143">
        <property type="term" value="P:tRNA wobble position uridine thiolation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01526">
        <property type="entry name" value="RHOD_ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00757">
        <property type="entry name" value="ThiF_MoeB_HesA_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.250.10:FF:000014">
        <property type="entry name" value="Adenylyltransferase and sulfurtransferase MOCS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000033">
        <property type="entry name" value="Adenylyltransferase and sulfurtransferase MOCS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03049">
        <property type="entry name" value="MOCS3_Uba4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028885">
        <property type="entry name" value="MOCS3/Uba4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004281">
        <property type="entry name" value="PRK05690.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF102">
        <property type="entry name" value="ADENYLYLTRANSFERASE AND SULFURTRANSFERASE MOCS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Rhodanese" evidence="11">
        <location>
            <begin position="369"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="26"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7729938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="474" mass="51482" checksum="F20040733855CC85" modified="2024-05-29" version="1" fragment="single">LNGVKLNPSMESNGGVDESSRLLHELQSLNDSKRDIEARISVIEAKLQQIQSNQHSIKDSYSVCSKGVSEYHDLTPDMIYRYSRQLLLPSFGVQGQSNLLKSSILVIGAGGLGSPALLYLAACGVGRLGMVDHDVVELNNLHRQIIHGEAYIGRSKVESAAAACRAINSTVEIVEHKEALRTSNALEIVSKYDIVIDATDNAPSRYLISDCCVLLGKPLVSGAALGLEGQLTVYNYNGGPCYRCLFPTPPPTTACQRCSDSGVLGVVPGVIGCLQALEAIKLASLVGEPLSGRMLLFDALSARIRIVKIRGRSLQCEACGENALTQQQFQQFDYEKFTQSPLAVAPLKLKLLTSDFRISSKEYSELIKKGSPHVLIDVRPSHHYKITSLPKSINIPLANLESRLPEISSELKSVQESSGTLNGSDAGLYVVCRRGNDSQRAVQLLHKMGFASAKDIVGGLESWAREVDQKFPTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BYD3</accession>
    <name>A0AAD5BYD3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">mannan endo-1,4-beta-mannosidase</fullName>
            <ecNumber evidence="4">3.2.1.78</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_017182</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random hydrolysis of (1-&gt;4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.</text>
            <dbReference type="EC" id="3.2.1.78"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyl hydrolase 5 (cellulase A) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.78" evidence="4"/>
    <dbReference type="EMBL" id="JAMZMK010010342">
        <property type="protein sequence ID" value="KAI7732003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BYD3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016985">
        <property type="term" value="F:mannan endo-1,4-beta-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000012">
        <property type="entry name" value="Mannan endo-1,4-beta-mannosidase 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001547">
        <property type="entry name" value="Glyco_hydro_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045053">
        <property type="entry name" value="MAN-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31451">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31451:SF60">
        <property type="entry name" value="MANNAN ENDO-1,4-BETA-MANNOSIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26410">
        <property type="entry name" value="GH5_mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042023606" description="mannan endo-1,4-beta-mannosidase" evidence="8">
        <location>
            <begin position="23"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 5" evidence="9">
        <location>
            <begin position="32"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF26410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7732003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="447" mass="51237" checksum="BCEDCB6DB457CE18" modified="2024-05-29" version="1">MTNLYPILFLFPFFLLIQQQWCLQQLAYMDDDFVHINGAQFTLNGAPFYANGFNAYWLMEVASNPSQRPKVTSAFREAIKSSLLIGRTWAFSDGGPNALQSSPGVYNEDMFQGLDFVIYEAGRFGVKLILCLVNNFNDYGGKEQYVQWAKERGQQLQNEESFFTNLMVKSFFKDHIKTVLTRRNTITGVMYKDDPTIMAWELMNEPRCPSDISGATIQNWTLEMACYLKSMDGNHLLEVGLEGFYGASSSQKNPNNSTYGADFIANNQIPEVDFATVHSYPDQWLSNQDDAAQLDFLQQWIHDHIEDAQSILKKPVLFTEFGKSWKHPGYNVSQKDQLYHVVYSSIYRSARTNGVAVGGLFWQQLVQGMDSYKDGYEVILTEPSSTVSLISRQSKKLSVTHKRFLIKKRTQAPRAKRLMQTNWIRNGLQIHAHDEIIKLYKQQGDMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BYI0</accession>
    <name>A0AAD5BYI0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Dof zinc finger protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_031357</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Transcription factor that binds specifically to a 5'-AA[AG]G-3' consensus core sequence.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010316">
        <property type="protein sequence ID" value="KAI7732078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BYI0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045174">
        <property type="entry name" value="Dof"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003851">
        <property type="entry name" value="Znf_Dof"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31992:SF295">
        <property type="entry name" value="DOF ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31992">
        <property type="entry name" value="DOF ZINC FINGER PROTEIN DOF1.4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02701">
        <property type="entry name" value="Zn_ribbon_Dof"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01361">
        <property type="entry name" value="ZF_DOF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50884">
        <property type="entry name" value="ZF_DOF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 8 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3 9" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 8 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Dof-type" evidence="11">
        <location>
            <begin position="74"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="14"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="115"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="250"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="18"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="32"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="124"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="146"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="262"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU369094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7732078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="321" mass="34346" checksum="0FB56408FBCD24EA" modified="2024-05-29" version="1">MPVISLSIEKLHQSNHQGSGGVGGGGVGENPNLPPPPPPPAQTGGGGEGTGTIRPGSMVDRARLAKIPLPEPGLNCPRCDSTNTKFCYFNNYNLTQPRHFCKTCRRYWTRGGALRNVPVGGGCRRNKRSSKSRSSKSPRHSGPKSVSVSPPRSTNSENMTSTQLPRPPTLHLPFMSSLNQYGGLGSSNIAGFHPPSDQIGNFQMGNNNGNILSIGGGDNWRLPFFPGFEVPNNTNNNNLFNYQTEGVGAQSSSMVGGDSRLKNSSGVDNPSEGSVKMEDNRRQFLGMLGNNNNQQAAWSGNANNWVEFPGVNISPSTTHFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BZQ5</accession>
    <name>A0AAD5BZQ5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glutathione transferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_012528</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GST superfamily. Zeta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010213">
        <property type="protein sequence ID" value="KAI7732472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BZQ5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016034">
        <property type="term" value="F:maleylacetoacetate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006559">
        <property type="term" value="P:L-phenylalanine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03191">
        <property type="entry name" value="GST_C_Zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00570">
        <property type="entry name" value="GST_N_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000017">
        <property type="entry name" value="Maleylacetoacetate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005955">
        <property type="entry name" value="GST_Zeta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034330">
        <property type="entry name" value="GST_Zeta_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01262">
        <property type="entry name" value="maiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42673:SF18">
        <property type="entry name" value="GLUTATHIONE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42673">
        <property type="entry name" value="MALEYLACETOACETATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14497">
        <property type="entry name" value="GST_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13417">
        <property type="entry name" value="GST_N_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST N-terminal" evidence="2">
        <location>
            <begin position="8"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="3">
        <location>
            <begin position="90"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7732472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="226" mass="25633" checksum="07B4FEEB93E0FB8F" modified="2024-05-29" version="1">MEEGNSTTMMKLYSHPMSSCSRRVRLALYLKGLNYECINITSIGDPELLKVNPMGYVPALVDGTTVVSDSYAILLYLEEKYPQHALLPQDIMKKSINYQAASIVSSSIQPLQNTTLVHYIGEIVSPDEKLPWAQYHIRKGFVALEKLLANHAGKYATGDEIYLADLYLAPQLDNAINRFKLDMTEFPLLLKLYKIYCETPSFQQIFAEPPQQIDKSLFNFTKPQEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C017</accession>
    <name>A0AAD5C017_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_000471</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-lysyl(27)-[histone H3] + 3 S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl(27)-[histone H3] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:60292"/>
            <dbReference type="Rhea" id="RHEA-COMP:15535"/>
            <dbReference type="Rhea" id="RHEA-COMP:15548"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="EC" id="2.1.1.356"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010421">
        <property type="protein sequence ID" value="KAI7731599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C017"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031519">
        <property type="term" value="C:PcG protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140951">
        <property type="term" value="F:histone H3K27 trimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031507">
        <property type="term" value="P:heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050793">
        <property type="term" value="P:regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd10519">
        <property type="entry name" value="SET_EZH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.270.10:FF:000001">
        <property type="entry name" value="Putative histone-lysine N-methyltransferase EZH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026489">
        <property type="entry name" value="CXC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045318">
        <property type="entry name" value="EZH1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025778">
        <property type="entry name" value="Hist-Lys_N-MeTrfase_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058609">
        <property type="entry name" value="HTH_CLF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041355">
        <property type="entry name" value="Pre-SET_CXC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033467">
        <property type="entry name" value="Tesmin/TSO1-like_CXC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45747">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE E(Z)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45747:SF14">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE EZA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25996">
        <property type="entry name" value="HTH_CLF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18264">
        <property type="entry name" value="preSET_CXC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01114">
        <property type="entry name" value="CXC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51633">
        <property type="entry name" value="CXC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51576">
        <property type="entry name" value="SAM_MT43_EZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CXC" evidence="9">
        <location>
            <begin position="563"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="domain" description="SET" evidence="8">
        <location>
            <begin position="676"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="345"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="517"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="808"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="373"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="521"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="811"/>
            <end position="821"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7731599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="831" mass="92999" checksum="A405419EAC309DD5" modified="2024-05-29" version="1">MMVSTSNSIDSSKSSNSRRGQASADLEFLKVRINQLKKQIQAERILLIKEKVEKNSEKLENDVSHLLSLSAHRADDSANENSGMGEMLSSRIKNPLCILTGPGQISGEDNNSSQEAAISISAKLPVADKIPPYTTWIFLARNQRMADDQSIVGRRRIYYDKNGSEALICSDSEEELTEPEEEKHEFSETEKRIIRMVSQDYEPSDEVIKILTQYVGGNTSEIYEAYIMQKTQDEGDVNQSVSASADHEHEWSIPLDRSLSASLDSLDNLFCRRCLVFDCRLHGYSQTVITPTERQHYPLESQENVKPCSDHCYLQFQTVKKHSDHLDVSSETDNTPSDEVLHKIQIGGDPGKRKASKQTTTVLKEPKHVIDDQQGSSLKKQKTVSVPDTISVTVDDTQVSDDILNDKTMDRQMATNDEATSSSKCVLKDVINDRGIGVNLLKQESTPVGAKIVSPPVDCKWKPLEKELYVKGLEIFGKNSCLIARNLLSGLKTCIEVANYMGDNGAATSQRSFTACSMPDGSERADADPKEQDTASRSRLLRRKGRSKKLKYSWKSAGHPSMWRRIADEKNQSCKQYIPCTCKSMCGKECPCLDNATCCEKYCGCSKSCKNRFRGCHCAKSQCRSRQCPCFAAGRECDPDVCRNCWMSCGDGSSGEPPRRGEGHCGNMRLLLRQQQRILLAKSSVAGWGAFLKNSVNKNDYLGEYTGELISHREADKRGKIYDRANSSFLFDLNDQANLKLTVLAYVLDAYRKGDKLKFANHSSNPNCYAKVMMVAGDHRVGIFAKERIEAGEELFYDYRYGPDQAPIWARKPDGSNRDDLLVPQGRAKKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C2G9</accession>
    <name>A0AAD5C2G9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_027121</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the glycosyl hydrolase 28 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009906">
        <property type="protein sequence ID" value="KAI7733785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C2G9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004650">
        <property type="term" value="F:polygalacturonase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.10:FF:000004">
        <property type="entry name" value="Pectin lyase-like superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000743">
        <property type="entry name" value="Glyco_hydro_28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31375">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00295">
        <property type="entry name" value="Glyco_hydro_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00710">
        <property type="entry name" value="PbH1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00502">
        <property type="entry name" value="POLYGALACTURONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0134">Cell wall</keyword>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6 9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042133324" evidence="10">
        <location>
            <begin position="22"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7733785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="396" mass="43018" checksum="B17F7AA2638FF682" modified="2024-05-29" version="1">MTGSKATIVFALFTFIAVSKAHVFDVTKFGARPGCEISKALGAAWKAACCVPKHSLLIIPKGQFCLNWFEFRGPCKGSIEVRIDAILTGPKDPAHIPKGKQWITFSYLNGFTLSGCGTLDGVGGAMAWPSNNPKINAHRRKPCGLQYNLSFNFIQNSLITGITSKDSKNFHSNVMSCTNVTFDNIQIVAPAESPNTDGIHLGWSKHITIKNSVIRTGDDCISIGDGCENLHIEGVKCGPGHGISVGSLGRNRGEKPVRGVFVKKCLFTCTENGVRIKTWPDSCPGEVADMNFEDLTMDKVQNPIIIEQDYCPHGACIKHKPSLVKVHKVLINNIQGTATTPAVVKLRCSKANRGCENVKISNINLRYVGPHGHAFQECCNVRPIYSGLMLPPGCRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C2T0</accession>
    <name>A0AAD5C2T0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DRBM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_021321</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010027">
        <property type="protein sequence ID" value="KAI7733328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C2T0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004525">
        <property type="term" value="F:ribonuclease III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014720">
        <property type="entry name" value="dsRBD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11207:SF1">
        <property type="entry name" value="DOUBLE-STRANDED RNA-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11207">
        <property type="entry name" value="RIBONUCLEASE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00035">
        <property type="entry name" value="dsrm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50137">
        <property type="entry name" value="DS_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DRBM" evidence="4">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="87"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="87"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="99"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7733328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="150" mass="16764" checksum="8B42913011D0E7A7" modified="2024-05-29" version="1">MNYAIPSYVCTRDETKGRDAPFSCTVDIGGMKYIGTAAKTKKEAELKAAKIALLAIKMSTPEPNGELDTPVQESVYTVVPIKRKEPERPVVETEEKVKTNKRKKGKFKRRKRKGEMARGEVVEVKMNDDDLKSNGESVLVVSEDQGLFLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C315</accession>
    <name>A0AAD5C315_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_031081</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009792">
        <property type="protein sequence ID" value="KAI7734182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C315"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052779">
        <property type="entry name" value="WDR62"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45589">
        <property type="entry name" value="WD REPEAT DOMAIN 62, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45589:SF1">
        <property type="entry name" value="WD REPEAT DOMAIN 62, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7734182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="91" mass="9819" checksum="3163079640EB0229" modified="2024-05-29" version="1" fragment="single">MKPNRKPKKPQTPYSKFDLEEIIGLTVTNANGLGCSKFDSKFAYTAGCVVVLYDVDLGTQLHFVVSSRLPKPLGCVAVSHDGTYIAAGEVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C8D5</accession>
    <name>A0AAD5C8D5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">WEB family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_021514</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WEB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009183">
        <property type="protein sequence ID" value="KAI7736830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C8D5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009904">
        <property type="term" value="P:chloroplast accumulation movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009903">
        <property type="term" value="P:chloroplast avoidance movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008545">
        <property type="entry name" value="Web"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32054">
        <property type="entry name" value="HEAVY CHAIN, PUTATIVE, EXPRESSED-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32054:SF36">
        <property type="entry name" value="WEB FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05701">
        <property type="entry name" value="WEMBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="329"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="474"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="59"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="329"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7736830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="494" mass="55669" checksum="FEA4051EDAF6EAE3" modified="2024-05-29" version="1">MTETIQPSIVLGNSETGKLMNPRVEIDTSPPFGSVKEAVTRFGGSGSWIPLHILRLAGHDIGEIDMETVEKQAAELEKELITKEQETLEVLRELESAKGVMEGLKLNIMKEVSSVTSSDNSSASLTLCSVPLPPAVILSELKEAKSKLSRTTNDLAIIRASVESLNKKMRTHKVHLAENEETKGVIEESIDVSNHGSHKLTFEAEQFRRMEEAAQYEVIKATSEIEHTKMSIKMVEMRLIAAKKMEEAARAMEAVACAESKPFVSEGITLSYEEYRELAQKARKAEEVSKKNDYHRKDANLSTMAFLKNAMDQTDSAKFRRNTLEDALGHKTDAANGRNHESKNGISPRKPETHNHPKTRNSYPFRGHAPRRFEPLIGDYPSMINKESRPVLRTSVSIGDILNKKLILQDNIIVHEDVETQTRRDDASLSEMLRDQSGLMFHDPAKTDKETRNDKQFFSQRKKFGFIHVSLPMTKHNKKKSNPQTPQPLNMRFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C8F0</accession>
    <name>A0AAD5C8F0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_025710</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009232">
        <property type="protein sequence ID" value="KAI7736625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C8F0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11072">
        <property type="entry name" value="CYP71-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000054">
        <property type="entry name" value="Cytochrome P450 84A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053062">
        <property type="entry name" value="CYP450_84A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47945">
        <property type="entry name" value="CYTOCHROME P450 84A1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47945:SF5">
        <property type="entry name" value="CYTOCHROME P450 84A1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 9 10" id="KW-0349">Heme</keyword>
    <keyword evidence="7 9 10" id="KW-0408">Iron</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 9 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="9">
        <location>
            <position position="444"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7736625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="507" mass="57452" checksum="FAEFD31D7123F3A6" modified="2024-05-29" version="1">MELMQLTYYLITLSLTLFLLSWLPRKSQKPLPPGPPGWPVIGNLLMMGQLTHRGLAHLSAQYGGIIYLKMGFRHTIAICSPEMAKQVLQIQDNIFSDRPTTIAITYLTYNRVDMAFGDYGPFWRQMRKLSVMKLFSRKRAESWNPVREEVDSMVKTTAANSGSRVNIGNQVFGLTHDIIYRAAFGSVSLEGKQEFIGILQEYTKLFSAFNLADFIPWLGFVDPSGLNTRLPAARSSLDRFIDKIIDEHLTREKKTGDDNMDSDMVDEMLAFFSDDQEVEHDEDLHGSIKLTRNNIKAIIMDIMFGGTETVASAIEWAMAELMHTPDAMKRVQQELEDIVGLDRRVEESDFEKLTYFKCVIKETLRLHPPIPVALHQSSAATRIGDYHIPKGTRVMINFYAINRDKGAWKDPNVFDPSRFLQKGSPDFKGSNYEFLPFGSGRRSCPGMQLGLYTMEMAVAHLLHCFTWELPDEMKPSEIDMSDVFGLTAPKASRLVAVPTPRLSCPIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C9M2</accession>
    <name>A0AAD5C9M2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GH16 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_020042</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009071">
        <property type="protein sequence ID" value="KAI7737285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016762">
        <property type="term" value="F:xyloglucan:xyloglucosyl transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042546">
        <property type="term" value="P:cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010411">
        <property type="term" value="P:xyloglucan metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000757">
        <property type="entry name" value="Beta-glucanase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044791">
        <property type="entry name" value="Beta-glucanase/XTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016455">
        <property type="entry name" value="XTH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31062">
        <property type="entry name" value="XYLOGLUCAN ENDOTRANSGLUCOSYLASE/HYDROLASE PROTEIN 8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00722">
        <property type="entry name" value="Glyco_hydro_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005604">
        <property type="entry name" value="XET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51762">
        <property type="entry name" value="GH16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042096121" description="GH16 domain-containing protein" evidence="4">
        <location>
            <begin position="19"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="GH16" evidence="5">
        <location>
            <begin position="17"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="113"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="3">
        <location>
            <position position="117"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR005604-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7737285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="268" mass="30270" checksum="4367F962E5822564" modified="2024-05-29" version="1" fragment="single">MAFFLSILLFMFTISTDAQGNPPSPGYNPSSTVDTVTFDQAYRTLWGQQNQRFDQSSLTIWLDKSSGSGFKSIKSYXXXXFGTSVKLHPGYTAGVITSFYLSNSEDHPGDHDEIDIEFLGTTPDKPYTLQTNVYMRGSGDGNIIGREHRIHLWFDPTQDFHDYAILWTPNEIIFFVDDVPIRSYPRKSDATFPLRPMYVYGSIWDASSWATEDGRYKANYGYQPFVGRYRNFKLKGCEADSAQTCRPPSGSPATTGGLSLQQVEAMQW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C9T0</accession>
    <name>A0AAD5C9T0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ceramide glucosyltransferase</fullName>
            <ecNumber evidence="5">2.4.1.80</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_017026</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyltransferase 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.80" evidence="5"/>
    <dbReference type="EMBL" id="JAMZMK010008997">
        <property type="protein sequence ID" value="KAI7737535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C9T0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008120">
        <property type="term" value="F:ceramide glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006679">
        <property type="term" value="P:glucosylceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025993">
        <property type="entry name" value="Ceramide_glucosylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12726">
        <property type="entry name" value="CERAMIDE GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12726:SF0">
        <property type="entry name" value="CERAMIDE GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="26"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="325"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="366"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7737535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="501" mass="56591" checksum="DB3113FEB40D5403" modified="2024-05-29" version="1">MIDIMSALESIDSLLFSISRALCTPFAVFVQIQGCVICLLLAAGWAGAAYVRNREIRQMKKSMKDGNPFAFLSHDIHDLEHSNQVKLPGVTVVMPLKGFGEHNLHNWRTQLTSLYGGPLEFLFIVDSTEDPAYHAVSRLLLDFKDDVDARLIVAGPSTTCSQKVHNQLVGVEKMHKDSKYVLFLDDDVRLHPGSIGALTAEMIKNPEIFIQTGYPLDLPSGSLGSYCIYEYHMMHADDFRTDKHGVVTQLSDGGYSDDMTLAAIAGANKRLITSPPVAVFPHPLASDLTFSRYWNYLRKQTFVLESYTTYVNWIMNRALFSVHCYLSWGFVSPYIMAMFHVAAALRFHFKHISAEEHVLNPNGLRLVGCLVICTVIELLSMWNLTRIEVRLCNMLSPEAPPLSLSAYNWCLVFVAMVVDNFLYPISAIRSHFSQSINWSGIRYHLRNGKIHKIDRTMEKGKKFSDLAAKRLYGRKAQSKISILGTLSRSLANWRQPKKYDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CAB1</accession>
    <name>A0AAD5CAB1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Chlorophyll a-b binding protein, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">M8C21_022823</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="20">AA19_3_7</strain>
            <tissue evidence="20">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="19">The light-harvesting complex (LHC) functions as a light receptor, it captures and delivers excitation energy to photosystems with which it is closely associated.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast thylakoid membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 19">Belongs to the light-harvesting chlorophyll a/b-binding (LHC) protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008916">
        <property type="protein sequence ID" value="KAI7737977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CAB1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009522">
        <property type="term" value="C:photosystem I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009523">
        <property type="term" value="C:photosystem II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016168">
        <property type="term" value="F:chlorophyll binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009765">
        <property type="term" value="P:photosynthesis, light harvesting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3460.10:FF:000006">
        <property type="entry name" value="Chlorophyll a-b binding protein, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3460.10">
        <property type="entry name" value="Chlorophyll a/b binding protein domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001344">
        <property type="entry name" value="Chloro_AB-bd_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022796">
        <property type="entry name" value="Chloroa_b-bind"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21649">
        <property type="entry name" value="CHLOROPHYLL A/B BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00504">
        <property type="entry name" value="Chloroa_b-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103511">
        <property type="entry name" value="Chlorophyll a-b binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 18 19" id="KW-0148">Chlorophyll</keyword>
    <keyword evidence="4 19" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="14 19" id="KW-0157">Chromophore</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5 19" id="KW-0602">Photosynthesis</keyword>
    <keyword evidence="10 19" id="KW-0603">Photosystem I</keyword>
    <keyword evidence="17 19" id="KW-0604">Photosystem II</keyword>
    <keyword evidence="7 19" id="KW-0934">Plastid</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15 19" id="KW-0793">Thylakoid</keyword>
    <keyword evidence="12" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="binding site" evidence="18">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>chlorophyll a</name>
            <dbReference type="ChEBI" id="CHEBI:58416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>chlorophyll a</name>
            <dbReference type="ChEBI" id="CHEBI:58416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="18">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>chlorophyll a</name>
            <dbReference type="ChEBI" id="CHEBI:58416"/>
            <label>2</label>
        </ligand>
        <ligandPart>
            <name>Mg</name>
            <dbReference type="ChEBI" id="CHEBI:25107"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>chlorophyll a</name>
            <dbReference type="ChEBI" id="CHEBI:58416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>chlorophyll a</name>
            <dbReference type="ChEBI" id="CHEBI:58416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>chlorophyll a</name>
            <dbReference type="ChEBI" id="CHEBI:58416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>chlorophyll a</name>
            <dbReference type="ChEBI" id="CHEBI:58416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>chlorophyll a</name>
            <dbReference type="ChEBI" id="CHEBI:58416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>chlorophyll a</name>
            <dbReference type="ChEBI" id="CHEBI:58416"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR601344-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU363080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="KAI7737977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="273" mass="29486" checksum="CACDF61C772CDC0C" modified="2024-05-29" version="1">MATQALASSSSLTSSVESARQILGARPLHTSSRKVSFVVKAASTPPVKQGANRQLWFASKQSLSYLDGSLPGDYGFDPLGLSDPEGTGGFIEPKWLAYGEVINGRFAMLGAAGAIAPEIFGKLGLIPPETALPWFKTGVIPPAGTYEYWADPFTLFVFEMALMGFAEHRRLQDWYNPGSMGKQYFLGLEKGFAGSGDPAYPGGPFFNPLGFGKDEKSMKELKLKEIKNGRLAMLAILGYFIQGLVTGVGPFQNLLDHLADPVNNNVLTSLKFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CBT5</accession>
    <name>A0AAD5CBT5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_015832</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the iron/ascorbate-dependent oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008797">
        <property type="protein sequence ID" value="KAI7738554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CBT5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.330:FF:000001">
        <property type="entry name" value="Protein SRG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050295">
        <property type="entry name" value="Plant_2OG-oxidoreductases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47991">
        <property type="entry name" value="OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0408">Iron</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="6">
        <location>
            <begin position="192"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7738554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="350" mass="40241" checksum="57A80529EED8674F" modified="2024-05-29" version="1" fragment="single">VQELAKELKLDHVPKLYIQENREPTFVCNHSLPLPSIPVIDMNDFNNTLESDSEPLKNLRSVCREWGIFQLVNHGVDKLLIKKMKEEVVNFFNMPLEEKLRYKLMAGDYQGYGQTILHKLDQKVDWADRFYMITNPVHRRKSNLLPEFPPVLRDTMENYLRELQKLAMYLFGLTGQAVKIDKQEMIDVFEDGMQSVRMTYYPPCPQPDLVIGLTPHSDAAGITILLQVNEVEGLEVKKDGVWIPVNFLPDAFVVNIGDILEIMSNGVYNSIEHRATVNATKERMSLAMFFNPKLEADVGPAKSLTTNTSDPPLYKTLVMEQYLKDFFSRKLNGKTFLERMKIGYDKASDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CBV0</accession>
    <name>A0AAD5CBV0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_005882</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008962">
        <property type="protein sequence ID" value="KAI7737749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CBV0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7737749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="100" mass="11101" checksum="D454B7073A0D4779" modified="2024-05-29" version="1">MATELLSNTSRLISLKPTKLSTDRPNGSYYSSRTSSCMLFSDRVVNRGCCLRNGVKHVSALVKESCVGSMEKGYGFGLNRVWDDLVKKNDDKVCEVGLIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CEL2</accession>
    <name>A0AAD5CEL2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">beta-aspartyl-peptidase</fullName>
            <ecNumber evidence="3">3.4.19.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_003685</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleavage of a beta-linked Asp residue from the N-terminus of a polypeptide.</text>
            <dbReference type="EC" id="3.4.19.5"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterotetramer of two alpha and two beta chains arranged as a dimer of alpha/beta heterodimers.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.5" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010008424">
        <property type="protein sequence ID" value="KAI7740461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CEL2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008798">
        <property type="term" value="F:beta-aspartyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003948">
        <property type="term" value="F:N4-(beta-N-acetylglucosaminyl)-L-asparaginase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000246">
        <property type="entry name" value="Peptidase_T2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10188">
        <property type="entry name" value="L-ASPARAGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10188:SF6">
        <property type="entry name" value="N(4)-(BETA-N-ACETYLGLUCOSAMINYL)-L-ASPARAGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01112">
        <property type="entry name" value="Asparaginase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041944899" description="beta-aspartyl-peptidase" evidence="5">
        <location>
            <begin position="21"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7740461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="100" mass="10383" checksum="E9837288AFDA9B36" modified="2024-05-29" version="1">MAGVLPLLIIMVVLSSKVTGHEVENSSKFPLVVSTWPFLEAVRAGWRAINGGLSSIDAVVEGCSACELLRCDGTVGPGGSPDENSESTIDALIMNGVTTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CEP3</accession>
    <name>A0AAD5CEP3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_026814</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the importin alpha family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008547">
        <property type="protein sequence ID" value="KAI7739890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CEP3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032413">
        <property type="entry name" value="Arm_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23316">
        <property type="entry name" value="IMPORTIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16186">
        <property type="entry name" value="Arm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7739890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="232" mass="26209" checksum="CAC7CAB5FA0F75F7" modified="2024-05-29" version="1">METSMERSVASLASSTPIIRSNRSHQLNGLYWPFVDPTFKHQTGRCIIQQDKRDAFYSWRPVHTYYQSKGLIGFFHCYTSMERSVASLASSTAGLVCLCSIMIIRNLAFLQYLCEQLHNKSWKKVNAYKSSGHAAMLCRQSLKCKLSEVEKQAGNSGDVNYFAQLIDDAEGLEKIENLQSHDNNEIYEKAVKILETYWLDEEDENVADGAQTSFDFGGNNVQVPSGGFNFNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CF05</accession>
    <name>A0AAD5CF05_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL4m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_021968</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008327">
        <property type="protein sequence ID" value="KAI7740868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CF05"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1370.10:FF:000006">
        <property type="entry name" value="50S ribosomal protein L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1370.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01328_B">
        <property type="entry name" value="Ribosomal_uL4_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002136">
        <property type="entry name" value="Ribosomal_uL4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013005">
        <property type="entry name" value="Ribosomal_uL4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023574">
        <property type="entry name" value="Ribosomal_uL4_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03953">
        <property type="entry name" value="rplD_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10746">
        <property type="entry name" value="50S RIBOSOMAL PROTEIN L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10746:SF6">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL4M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00573">
        <property type="entry name" value="Ribosomal_L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52166">
        <property type="entry name" value="Ribosomal protein L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="152"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="152"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00040565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7740868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="322" mass="36128" checksum="FEA06A24401B49A9" modified="2024-05-29" version="1">MALRCSRRLLPTVVSSYKLHCNNNLDVVRRPLHILSNALHDHEIGDTQCSLEYSILRKAGFSLMGARALCTSMLSSESSEGSFPSDLLSRKQVLTPERAIGQLQDLVIPVTNFHNEDKGMMVLAGDVFDVPIRKDIIHRVVRWQLAKRQQGTHSTKTISEVSGTGRKPWPQKGTGRARHGTLRGPQFRHGAVMHGPKPRSHAFKLNKKVRRLGLKIALSARAAEGKARYVQLLVFDDLELPTHKTKNIVSYVKQMEETKKVLLVDGGPIDEKLKLATQNLHYVNVLPSIGLNVYSILLHDTLVMSRDAVNKIVERMHTPINR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CFZ8</accession>
    <name>A0AAD5CFZ8_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_019503</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008390">
        <property type="protein sequence ID" value="KAI7740625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CFZ8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7740625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="116" mass="13239" checksum="34D07C5519E372E6" modified="2024-05-29" version="1" fragment="single">GQVSETTEPINSSVPESDKGYTRGRSVVAKRKLFAVDNNDDHGERDIIFYEQDASEDDGNKHMDGLENEIDELVDEEFEVPEPENEAVRNVRKRVRGPTRMPEVWAQEKGDRIPIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CIE3</accession>
    <name>A0AAD5CIE3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glutathione S-transferase</fullName>
            <ecNumber evidence="3">2.5.1.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_031153</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Is involved in the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the GST superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010008204">
        <property type="protein sequence ID" value="KAI7741425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CIE3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03185">
        <property type="entry name" value="GST_C_Tau"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03058">
        <property type="entry name" value="GST_N_Tau"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000018">
        <property type="entry name" value="Glutathione S-transferase U20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000014">
        <property type="entry name" value="Tau class glutathione S-transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045074">
        <property type="entry name" value="GST_C_Tau"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045073">
        <property type="entry name" value="Omega/Tau-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11260:SF773">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE U26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11260">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE, GST, SUPERFAMILY, GST DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13410">
        <property type="entry name" value="GST_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01152">
        <property type="entry name" value="Main.3:_Omega-_and_Tau-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="4">
        <location>
            <begin position="5"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="5">
        <location>
            <begin position="91"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00047960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU369102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7741425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="224" mass="26250" checksum="F8D4F58B1EEBF12E" modified="2024-05-29" version="1">MAEQQQLVLLDFWASMFCMRVRIALAEKGIPYEFTEEDFLNNKSQLLLDMNPVHKKIPVLIHNGKPICESSIIVQYIDEVWNHKAPLLPSDPYAKAHARFWADFIDKKLYQIGRNLYTTKGEEHEASRTEFLECFKLLEAELGDKSYFAGETFGYVDVSLIPFYSWFQAYETYGNFNFEHECPKLVAWAKRCIKNKESVSKTLPELPKVYDFVQHLRKQYGIND</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CJ25</accession>
    <name>A0AAD5CJ25_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ADF-H domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_001564</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the actin-binding proteins ADF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007967">
        <property type="protein sequence ID" value="KAI7742522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CJ25"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030042">
        <property type="term" value="P:actin filament depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11286">
        <property type="entry name" value="ADF_cofilin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000025">
        <property type="entry name" value="Actin-depolymerizing factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002108">
        <property type="entry name" value="ADF-H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017904">
        <property type="entry name" value="ADF/Cofilin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11913">
        <property type="entry name" value="COFILIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00241">
        <property type="entry name" value="Cofilin_ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00102">
        <property type="entry name" value="ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51263">
        <property type="entry name" value="ADF_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ADF-H" evidence="3">
        <location>
            <begin position="21"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7742522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="151" mass="17304" checksum="EC8574F00817C48C" modified="2024-05-29" version="1">MNQEAKLEFKTNLGQANAASGMAVNDECKLKFSELKSKRNYRFIVFKIEQQQVVVEKVGSPDESYEDFTNSLPANECRYAVFDFDFTTDENCQKSKIFFIAWAPDTSKVREKMVYASSKDRFKRELDGIQVEVQATDPSEMSLDIVKARAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CMV2</accession>
    <name>A0AAD5CMV2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein uS15c</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_033079</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS15 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007464">
        <property type="protein sequence ID" value="KAI7744677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CMV2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00353">
        <property type="entry name" value="Ribosomal_S15p_S13e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.10">
        <property type="entry name" value="S15/NS1, RNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01343_B">
        <property type="entry name" value="Ribosomal_uS15_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000589">
        <property type="entry name" value="Ribosomal_uS15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005290">
        <property type="entry name" value="Ribosomal_uS15_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009068">
        <property type="entry name" value="uS15_NS1_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00952">
        <property type="entry name" value="S15_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47546:SF3">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S15, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47546">
        <property type="entry name" value="S15/NS1, RNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00312">
        <property type="entry name" value="Ribosomal_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01387">
        <property type="entry name" value="Ribosomal_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47060">
        <property type="entry name" value="S15/NS1 RNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="30"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="101"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="200"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="31"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="43"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="64"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="140"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="159"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7744677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="373" mass="42130" checksum="8860DF6219CC44F7" modified="2024-05-29" version="1">MAATAIFIHFRSNRHRRFLLHNATSTVRFYSSSDDNSHNPNSRFSDIKSTLKTTENTRTTSFHNPIPNTNTNPNPKAASLEEISKSLSKFKRAAPVVSFQELYQRNKTPKPDTGSGSGSGSQGPDFQEIRRSLKGIGSNPARSQSATGLNSKLRPRNSIEMQSDSTAKGNVSNEAKTGLGRLYSYGDMGEKLRSLRPRNNKNVRFSLEELNERLQKVREMEEKDMSAGQFGPLRDSLQKLSSVEQTKKKEVSISGLLLGETTRLSFEPPKEYFHPDHMSSAEKQKLELKKVRDKFKVSESDCGSARVQVAQLTTKIKHLATVLHKKDKHSRKGLQAMVQKRKKLLKYLRRTDWESYCFCLAELGLRDSADYKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CS49</accession>
    <name>A0AAD5CS49_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Polycomb protein SUZ12-like zinc finger domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_029846</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007210">
        <property type="protein sequence ID" value="KAI7745521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CS49"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057540">
        <property type="entry name" value="Znf_SUZ12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22597">
        <property type="entry name" value="POLYCOMB GROUP PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22597:SF22">
        <property type="entry name" value="POLYCOMB GROUP PROTEIN EMBRYONIC FLOWER 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23320">
        <property type="entry name" value="Zn_SUZ12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Polycomb protein SUZ12-like zinc finger" evidence="3">
        <location>
            <begin position="101"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7745521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="218" mass="24852" checksum="969D3F3846BA0785" modified="2024-05-29" version="1" fragment="single">EGKKRCLLANVIKICPLSGTLISANISLLRNSSDSDHVCAQLSQFHEEEQREAEESLLVFFKPVDLYIILQHRAKNHPSFLQRCLYYKVQEKNNGRLVEPGNVTFNYKYYKNMLEKCEVTKNYGCPFCMAKCACYKGLKSHLLASHDHFHYEFVVHDGYEGVIVFAKTNTRASDIFGNMVHPTKDDCKPMRRRGRQTANQVGPLALDLKVLCNAESAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CTK4</accession>
    <name>A0AAD5CTK4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_014843</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily. Type-7 methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007004">
        <property type="protein sequence ID" value="KAI7746300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CTK4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.270">
        <property type="entry name" value="Methyltransferase, alpha-helical capping domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005299">
        <property type="entry name" value="MeTrfase_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042086">
        <property type="entry name" value="MeTrfase_capping"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31009">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE:CARBOXYL METHYLTRANSFERASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03492">
        <property type="entry name" value="Methyltransf_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="580"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7746300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="580" mass="67448" checksum="5716DFB7AC3F52D2" modified="2024-05-29" version="1" fragment="single">MVEVIDKTTLNIPPPEYRVYLNDLPTNDFNAVFKILPDFYKQLNMARCQKNESSVFIAGYPGTFYGRLFPDKCLHFIYSSYSLHWLSRIPPGLYDKDGRSINKGRFYISESSSLQVFKAYFDQFQEDFSLFLQSRSKELLPRGRMVLILLGRRDQSHVDRGNSFLWELLSRSFATLVSKGEVKQEKVDEYDSHFYAPSRNEIEGEVKKEGSFEIDRFEMFELEHYAGPYMILLFYEFKVMEALLFVGSKKASDLVKHITLKSLEETYISTTPESLGIADLGCSCGQNTLSTVREMVEVIDKTTLNIPPPEYRVYLNDLPTNDFNAVFKILPDFYKQLNMARCQKNESSVFIAGYPGTFYGRLFPDKCLHFIYSSYSLHWLSRIPPGLYDKDGRSINKGRFYISESSSLQVFKAYFDQFQEDFSLFLQSRSKELLPRGRMVLILLGRRDQSHVDRGNSFLWELLSRSFATLVSKGEVKQEKVDEYDSHFYAPSRNEIEDEVKKEGSFEIDRFEMFELEHYAGPYMSHGTAVAKSIRAIQESMISQHFGEEILDKLFEDYGSLIDEEMAIEDIKPINEYYIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CU17</accession>
    <name>A0AAD5CU17_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Coatomer subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">M8C21_016613</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">AA19_3_7</strain>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">COPI-coated vesicle membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006778">
        <property type="protein sequence ID" value="KAI7747330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CU17"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030126">
        <property type="term" value="C:COPI vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22948">
        <property type="entry name" value="Coatomer_WDAD_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.470:FF:000002">
        <property type="entry name" value="Coatomer subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000010">
        <property type="entry name" value="Coatomer subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006692">
        <property type="entry name" value="Beta-prop_COPA/B_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047312">
        <property type="entry name" value="Coatomer_alpha_WD-assoc_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010714">
        <property type="entry name" value="Coatomer_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050844">
        <property type="entry name" value="Coatomer_complex_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056176">
        <property type="entry name" value="TPR_COPA_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19876">
        <property type="entry name" value="COATOMER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19876:SF42">
        <property type="entry name" value="COATOMER SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04053">
        <property type="entry name" value="B-prop_COPA_B_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06957">
        <property type="entry name" value="COPI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23953">
        <property type="entry name" value="TPR_COPA_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="8" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="6 14" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="47"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="89"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="131"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="200"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="14">
        <location>
            <begin position="244"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="COPA/B second beta-propeller" evidence="16">
        <location>
            <begin position="340"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="domain" description="COPA/B TPR" evidence="18">
        <location>
            <begin position="624"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="domain" description="Coatomer alpha subunit C-terminal" evidence="17">
        <location>
            <begin position="812"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="858"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="859"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="19">
        <location>
            <position position="883"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00025536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF04053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF06957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF23953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAI7747330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="21">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="883" mass="99881" checksum="85ED86A5DC1A5568" modified="2024-05-29" version="1" fragment="single">MLTKFETKSNRVKGLSFHSKRPWILASLHSGVIQLWDYRMGTLIDRFDEHDGPVRGVHFHQSQPLFVSGGDDYKIKVWNYKLHRCLFTLLGHLDYIRTVQFHHENPWIVSASDDQTIRIWNWQSRTCISVLTGHNHYVMCALFHPKEDLVVSASLDQTVRVWDIGSLKKKTVSPADDILRLSQMNADFFGGVDAVVKYVLEGHDRGVNWASFHPTLPLIVSGADDRQVKIWRMNDAKAWEVDTLRGHMNNVSCVLFHSRQDIIVSNSEDKSIRVWDATKRTGLQTFRREHDRFWILGCHPEMNLLAAGHDSGMIVFKLERERPAFSVSGDSLYFVKDRFLRFYEYSSQKDTQILPIRRPGSNSLNQGPRTLSYSPTENAILICSDVDGGSYELYIIPKESITRGDTVQDAKRGVGGSAVFVARNRFAVLEKSTNQVLVKNLKNEIVKKSALPVAADAIFYAGNLLCRAEDRVYIFDLQQRLVLGDLQASFVRYVVWSSDMESVALLSKHSIIIADKKLVHRCTLHETIRVKSGSWDDNGVFIYTTLTHIKYCLPNGDSGIIKTLDTPVYITKIFGNTIFCLDRDGKNRPIAIDSTEYIFKLSLLKKRYDHVMSMIRNSELCGQAMISYLQQKGFPEVALHFVKDERTRFNLALESGNIQIAVASAKEIDEKDHWYRLGVEALRQGNSGIVEYAYQRTKNFERLSFLYLITGNLDKLSKMMKIAEVKNDVMGQFHNALYLGDVQERIKILVNAGHLPLAYATAKTHGLNDVVEDLAEKLQGNIPNLPSRSSSLLIPPTPVLCGGDWPLLRVMKGIFEGGLDNVGKGGNEEYEDAGDADWGEDLDIVDGENIQNGDISMALDDEGAPEEENEEGGWDLEDLELPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CVN1</accession>
    <name>A0AAD5CVN1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_008058</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006465">
        <property type="protein sequence ID" value="KAI7748769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CVN1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035200">
        <property type="entry name" value="Cdc24_OB2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035203">
        <property type="entry name" value="Cdc24_OB3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36033">
        <property type="entry name" value="NUCLEIC ACID-BINDING PROTEINS SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36033:SF1">
        <property type="entry name" value="NUCLEIC ACID-BINDING PROTEINS SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17245">
        <property type="entry name" value="CDC24_OB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17244">
        <property type="entry name" value="CDC24_OB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cell division control protein 24 OB" evidence="2">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="Cell division control protein 24 OB" evidence="1">
        <location>
            <begin position="178"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF17244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7748769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="403" mass="44506" checksum="5E0E3304C3A592B3" modified="2024-05-29" version="1">MLLGAQFCSDSLSSISIDDVNQGVSYSLYARIESIGSLEIQGKFGSLQRKQITLVDNDNVRIKFLLWGDQVVLANCFSVGSWLALDRPFVSSCSESTLESTEEICLEYGSVTQLYIVPFIRHEEQVSVASTQNHYQGSKKLTAAGPSQGPKVSQVTLPCDSRGAIDFSNYPFRLFVADLRDKMSNISIYGVVTDIKKPNIKESTFSLRIEDSTGGIWTNLHFVKSWSLGKVSIGHTVYIAGLTCSMTKSQSIEVSWDEKEGGCLFANLSCLPALLNSSCLHKASTLSDLSANNNINTHNIGFECNFCGCHYDDDIVPSFHLKVTIADDTGKVLAWCTGHTATELLQISPDEFFNLPEEEQIMYPSSLEHERFIVALVNSKQGTVARDEDDISIWEITQALKTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CWD6</accession>
    <name>A0AAD5CWD6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_004503</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006394">
        <property type="protein sequence ID" value="KAI7749219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CWD6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7749219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="87" mass="9713" checksum="2B7FFAD3AF4DBEFC" modified="2024-05-29" version="1" fragment="single">INRLKSLEINCVASLAKFKRTQFQQIILALDNTSVLHYLIEASRNIGLDISKQDSTKQAEHGCLTKFNGDLGWSVVAVKRADVRQGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CZ83</accession>
    <name>A0AAD5CZ83_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Acid phosphatase 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_013006</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the APS1/VSP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006093">
        <property type="protein sequence ID" value="KAI7750704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CZ83"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003993">
        <property type="term" value="F:acid phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07535">
        <property type="entry name" value="HAD_VSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005519">
        <property type="entry name" value="Acid_phosphat_B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010028">
        <property type="entry name" value="Acid_phosphatase_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014403">
        <property type="entry name" value="APS1/VSP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01675">
        <property type="entry name" value="plant-AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31284">
        <property type="entry name" value="ACID PHOSPHATASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31284:SF9">
        <property type="entry name" value="HAD SUPERFAMILY, SUBFAMILY IIIB ACID PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03767">
        <property type="entry name" value="Acid_phosphat_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002674">
        <property type="entry name" value="VSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR002674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7750704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="236" mass="27299" checksum="7B0987ADD35307C3" modified="2024-05-29" version="1">MSTKGLDHSHYGGRLVELSLSYCLSWRLAVEVNNIQGWRTVPIQCLYDVEVYMLGGQYASDLNLIVDQISSYVDEIVMSEDGMDAWILDVDDTCLSNIFYYKGKRFGGDPYDPQAFKEWASRGIARAIPPILRLFQKLIDRGFKVFLISGRDETTFEQPTLHNLHFQGFFGFERLILRSETYKGQSGMVYKSEMRRKLVEEGYRIWGNVGDQWSDLHGQFVGNRTFKLPNPMYFVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CZI3</accession>
    <name>A0AAD5CZI3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_027805</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005955">
        <property type="protein sequence ID" value="KAI7751193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CZI3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33474:SF27">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33474">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042147654" evidence="2">
        <location>
            <begin position="25"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7751193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="94" mass="10538" checksum="89C7BDE97C723D77" modified="2024-05-29" version="1">MAANSFYLIVTLLAFSHLLFMADAISTSGKVHRHMLHGKVEILASGHNKDQIINVEESSFLEDEFMSERMDLEKNDYPGPGANRNHTPKPPRGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D086</accession>
    <name>A0AAD5D086_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_005052</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005864">
        <property type="protein sequence ID" value="KAI7751643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D086"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006384">
        <property type="entry name" value="HAD_hydro_PyrdxlP_Pase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016965">
        <property type="entry name" value="Pase_PHOSPHO-typ"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01489">
        <property type="entry name" value="DKMTPPase-SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01488">
        <property type="entry name" value="HAD-SF-IB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20889">
        <property type="entry name" value="PHOSPHATASE, ORPHAN 1, 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20889:SF19">
        <property type="entry name" value="THIAMINE PHOSPHATE PHOSPHATASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06888">
        <property type="entry name" value="Put_Phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF031051">
        <property type="entry name" value="PyrdxlP_Pase_PHOSPHO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="16"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="18"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR031051-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR031051-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7751643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="249" mass="28395" checksum="26687F1690D8784B" modified="2024-05-29" version="1">MATANQPSKEMIIIWDFDRTIITDHCDRWVVLETGLTKLFHRLRLTLSWNSLMDRIMDELHAQGKTIQDFVNRLNNFTLDPQMISAIRSAHDLGCEMKVLSNANQFFIETILKNNGVYECFSGIISNPTVVDEEGRLRILPYHGVHFQPHGCKFCPANLCKGIVIDKIRDSDTKKRGIIYIGDGKDDLCPALKLSEEDHVMPKKRLTLYYMLTLATIPIMPKIHAWKDGEELNSILVGLLSPQTDAINQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D281</accession>
    <name>A0AAD5D281_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Heat stress transcription factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_023451</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the HSF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005931">
        <property type="protein sequence ID" value="KAI7751354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D281"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000057">
        <property type="entry name" value="Heat shock transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000232">
        <property type="entry name" value="HSF_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015">
        <property type="entry name" value="HEAT SHOCK TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015:SF463">
        <property type="entry name" value="HEAT SHOCK TRANSCRIPTION FACTOR A3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00447">
        <property type="entry name" value="HSF_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00056">
        <property type="entry name" value="HSFDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00415">
        <property type="entry name" value="HSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00434">
        <property type="entry name" value="HSF_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0346">Stress response</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HSF-type DNA-binding" evidence="13">
        <location>
            <begin position="85"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="347"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="355"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00055747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00081483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7751354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="381" mass="43513" checksum="B93552146A6ECA66" modified="2024-05-29" version="1" fragment="single">MDPLFKHPSSSSSTNSDAEIAVETKTEDGYVPKPLDCLQGTPIPPFLSKTYDLVDDPSLDRVISWSDTGASFIVWDPIEFSRKILPKNFKHNNLSSFVRQLNTYGFRKIDTDRWEFARESFLRGKRHLLKNIRRRRSLQAQQLTEESSCFSVEAEVGRLHKERMEMMQEVIDLQDQQRGTHQYMESVNQKLQAAEDRQKQMVSSLAKVLQIPKFKKEQRRVSAPQLVRKFIKHQPDDHNFGLNPIPVDNTVRDLDNQSETVPSRLHDLDSQELAQVNDPFSKEVLDFNLDYDAATMVKQEDLWSSDFETVSAMPNNLWNDAGNYEYPEFGLPGGEVSDFWNVADSGAENWMSGEPSDSQKTNQSFGCDLGPLGSLSSVRKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D3H4</accession>
    <name>A0AAD5D3H4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transferase, Chloramphenicol acetyltransferase-like domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_032276</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the plant acyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005404">
        <property type="protein sequence ID" value="KAI7753533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D3H4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31623">
        <property type="entry name" value="F21J9.9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31623:SF92">
        <property type="entry name" value="VINORINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02458">
        <property type="entry name" value="Transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7753533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="432" mass="48352" checksum="BD649BED5111A03F" modified="2024-05-29" version="1">MTMITKLQRSCLRSRQLHTITSQETIKPSSPTPPHLKTHNLSLVDQLVPSINIPLIFFYKYYTPNDTTILKKSLSQTLTSYYPLAGRFVAPNFSAPQIDCNDQGVEFFEASNDSQLSDFILKKDVDETMDQLIPSYQVGGYKNHPYLAEVQLTRFGCGGAAVVVSMSHKVGDGFTMGSFINHWAAVARGGSPKNPTFLSSKCKFKIPEFDLKGGDEAKYAIKRFVFPNSKLNELKKKVNVTVVNPTRVEVLSSLIFSCALSAATTVSGCLQPSNMFQIVNMRKKLIEQNRPDIAAGNIGTLAAVKIRKCGEMKLNEVIYKLRKQIEEPKEVRDVDEIGGLCLKRLSMLGLNFGCRTYIFSSLCWFPFYEVDFGWGKPVQVIVGNGVFDGSIIHFMDTPSREGIEAIVKLEEREMAIFQQDKQILAYSQDNFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D457</accession>
    <name>A0AAD5D457_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_021422</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005380">
        <property type="protein sequence ID" value="KAI7753588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D457"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="95"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7753588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="116" mass="13440" checksum="FA98CC749EC959CF" modified="2024-05-29" version="1">MEPFSIRRISVRAPATLKESTQPTQNDDALINLAFLLIKEELRNFQPPICSSNRPLPNVDKYYSTKGFTKYRDGEHTFLVIRQVVLDFASKYSELDMILLVFFFIILLVPIIICEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D573</accession>
    <name>A0AAD5D573_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">60S ribosomal protein L35</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_021418</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL29 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005380">
        <property type="protein sequence ID" value="KAI7753584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D573"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00427">
        <property type="entry name" value="Ribosomal_L29_HIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.310:FF:000002">
        <property type="entry name" value="60S ribosomal protein L35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="6.10.250.3450:FF:000001">
        <property type="entry name" value="60S ribosomal protein L35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00374">
        <property type="entry name" value="Ribosomal_uL29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001854">
        <property type="entry name" value="Ribosomal_uL29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045059">
        <property type="entry name" value="Ribosomal_uL29_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036049">
        <property type="entry name" value="Ribosomal_uL29_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00012">
        <property type="entry name" value="L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45722">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45722:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL29-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00831">
        <property type="entry name" value="Ribosomal_L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46561">
        <property type="entry name" value="Ribosomal protein L29 (L29p)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7753584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="123" mass="14321" checksum="55CC7199FBAAA681" modified="2024-05-29" version="1">MARIKVHELREKTKTDLFAQLKELKTELALLRVAKVTGGAPNKLSKIKVVRTGIAQVLTVISQTQKAKLREAYKNKKYLPLDLRPKKTRAIRRRLTKHQASLKTEREKKKEKYFPLRKYAIKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D7H3</accession>
    <name>A0AAD5D7H3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">glucose-6-phosphate 1-epimerase</fullName>
            <ecNumber evidence="3 5">5.1.3.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_019222</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>alpha-D-glucose 6-phosphate = beta-D-glucose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:16249"/>
            <dbReference type="ChEBI" id="CHEBI:58225"/>
            <dbReference type="ChEBI" id="CHEBI:58247"/>
            <dbReference type="EC" id="5.1.3.15"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the glucose-6-phosphate 1-epimerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.15" evidence="3 5"/>
    <dbReference type="EMBL" id="JAMZMK010003606">
        <property type="protein sequence ID" value="KAI7754432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D7H3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047938">
        <property type="term" value="F:glucose-6-phosphate 1-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09020">
        <property type="entry name" value="D-hex-6-P-epi_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008183">
        <property type="entry name" value="Aldose_1/G6P_1-epimerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025532">
        <property type="entry name" value="G6P_1-epimerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014718">
        <property type="entry name" value="GH-type_carb-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11122">
        <property type="entry name" value="APOSPORY-ASSOCIATED PROTEIN C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11122:SF45">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE 1-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01263">
        <property type="entry name" value="Aldose_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016020">
        <property type="entry name" value="PHexose_mutarotase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" evidence="6">
        <location>
            <position position="168"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR016020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR016020-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7754432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="298" mass="33946" checksum="24EACF3CC0055579" modified="2024-05-29" version="1">MSTSERTPVEVTKSVNGLEKVILREIHGSSVEVYLYGAHVTSWKNEHGEELLFVSSKETFKPPKPIRGGITICFPQFSNLGPLELHGFARSRFWAFDDDPPPLPPDVTNKVFLDLILRPGAEDLKIWPHSFEYRLRITLGPGGDLMLTSLIRNTGTDGKPFTFTFAYHTYFSVSDISEVRVEGLETLDYLDNCQKRERFTEQGDSLTFESEVDRIYLSTPTKIAVMDHEKKRTYLIRKDGLPDAVVWNPWDKKAKAMTDFGDDEYKHMVCVEAAAVEKPITLKPGEEWRGRQQLSVVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D7T7</accession>
    <name>A0AAD5D7T7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_030037</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005306">
        <property type="protein sequence ID" value="KAI7753711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D7T7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7753711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="64" mass="7516" checksum="7FA83C063E598389" modified="2024-05-29" version="1" fragment="single">MKQRCCLFNRLPNNNINLIITTTNNNLRHCNGVSWWLWKASQDLTRWCVSATLVVVLRLVVMDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D828</accession>
    <name>A0AAD5D828_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_020126</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005475">
        <property type="protein sequence ID" value="KAI7753305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D828"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36335">
        <property type="entry name" value="CHAPERONE DNAJ-DOMAIN SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36335:SF1">
        <property type="entry name" value="CHAPERONE DNAJ-DOMAIN SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="2">
        <location>
            <begin position="294"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="153"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="122"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7753305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="357" mass="39996" checksum="1AC6FE114E49D953" modified="2024-05-29" version="1">MKTLSRCRNNIEADNVVLIDVDTYDFDNVIVIDIPESHTKKQGGSSDKTRPRTYIYIDDDDDDGDESPCDDNAGADLNGERNFENGASSSRRYGLENPSPVKLSKGKRTYSGRGFNRQSDSDSSDDDSPDCEFMEDFSGKLKEQWERASLKRKGVLHNDHASSSKPYTDANSEFGVNRNKKHDQDSNVNGTSDEQKDESPNFDATGVEGGKDDEPLGEMPYTSSEEENEKNDLETAATADDDVPVENSIIGQREKLKETDEYKRALEEELLARQKALQRQDLKRLEISCLDMASLLRGLGIEVGKGPVALPNEVRAAYKRALLSFHPDRASGSDMRQQVEAEEKFKLISRMKEKFSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D8M3</accession>
    <name>A0AAD5D8M3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">IST1-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_002340</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the IST1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000384">
        <property type="protein sequence ID" value="KAI7756451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D8M3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1260.60:FF:000002">
        <property type="entry name" value="Vacuolar protein sorting-associated protein IST1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.60">
        <property type="entry name" value="Vacuolar protein sorting-associated protein Ist1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005061">
        <property type="entry name" value="Ist1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042277">
        <property type="entry name" value="IST1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12161">
        <property type="entry name" value="IST1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12161:SF44">
        <property type="entry name" value="REGULATOR OF VPS4 ACTIVITY IN THE MVB PATHWAY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03398">
        <property type="entry name" value="Ist1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7756451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="369" mass="42370" checksum="6371515AA7AD6F41" modified="2024-05-29" version="1">MFDFMFGWRKASKCKRLMKKLRGRVKLLKNKRCCVVRQLRNDVAQLIKHGHHQTAFNRVDQVYKDECIIAVYDLLAHFCEFLSRQLPYIRRSKDCPNDINEAISSLIFASARCGDLPELLRIRRLFSERYGERFEARAVDLLPGNLVNSKIRENLSINKVPNEVKYKLMEEITTTVLQTGPLALECYASEQGQLDASNGINDVHHGQHADVDFSPHQSNFSQDRSITYTDIEEFQPPLKDGETEKDQRVFMFSGVGNPKSINLLISIIIEKLADSIKVRRRSGKRTVSTDVLPPRNSEKLGVYSRAMTMPCEGWKELCVDEDGVMRSYSLPVQQCSPHVHPKLPDYDELAAKFMALKKQKFLNQEICSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D8T2</accession>
    <name>A0AAD5D8T2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_008744</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000352">
        <property type="protein sequence ID" value="KAI7756541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D8T2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7756541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="124" mass="14380" checksum="93BD591D77183624" modified="2024-05-29" version="1" fragment="single">TDLSKDTVKAFFPGPRGAAPLTKFISFKKLHQVKAKWEFKVVKVEEVVNYISVTRQIKQMWERHERIHCSKATQNVNWKGFDCATYEFKTSRWGELVLVAQFSLQDNGYSGGKNMSKLIATRRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DBL4</accession>
    <name>A0AAD5DBL4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_001383</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GMC oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000395">
        <property type="protein sequence ID" value="KAI7756424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DBL4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46056">
        <property type="entry name" value="LONG-CHAIN-ALCOHOL OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46056:SF10">
        <property type="entry name" value="LONG-CHAIN-ALCOHOL OXIDASE FAO3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="153"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7756424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="174" mass="19986" checksum="DC203CF893FA1D4E" modified="2024-05-29" version="1" fragment="single">MYDEREKECHPLLRGGERDEDYKKNGSLRSSEMESLTSLCEVILQPLPLNSLDDAHHHSHGNNMEESIRLFSTLSGSQNSVPNKVSNLIVKRGFFEAVMVVRLVLKLLSTRLGTLFLAGSLCLSHQWPYINKFSEIPLEKREKIVQKWFKNSLLTPIRLGFVFIKSLCLFVFFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DBV0</accession>
    <name>A0AAD5DBV0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_017559</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000310">
        <property type="protein sequence ID" value="KAI7756682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DBV0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45613:SF391">
        <property type="entry name" value="OS07G0621100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45613">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12854">
        <property type="entry name" value="PPR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="44"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="79"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="125"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="160"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="195"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="266"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="301"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="336"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="371"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="406"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7756682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="525" mass="59063" checksum="6CD95C2A71C96CFC" modified="2024-05-29" version="1">MSNIGVRSRFLSYGAVIESLVHFESPEYAVSVFGLILKSGFSVNTYLINIVLKGLCCKSKVDEAVSLFRQGAGYGVCPDEVTVNTLTSWLCKSGRFDEALDLKSGKIEKGKEVFDDMMRKGIVPSIEVYNYLVHGLAKKGKFKEAMDVLADMMERGIEPDVVTYAGLIDGLCYNGKVKKGLDVFDVMIERGQEPNNVTYMHLILGLCKEGSVVDGFNLLKTMIQKGKKRSKKFYDELIKELCDQRKVDDALMLYDMMNHESDVEPDVKTHNFIIQGLCKENRVTKAEEIHRKMVGNKLYGNVVTYNLLLDAYLRNGEIEKGMAIWKKLRQLKLSPNAYTFSLIINGFCKLGRINIAKGFFIKMRKHDLPLSVCDYNALMLALCTEGSVEQATKLFQEMESNNCKPDVASFNIIIDASFKSGNVEYTDELLVMMRDKGVEPDAYTFSILINRYCKLGELEEAGETDEIFKLLREMAEKGIALDSKPGASCTHGFRLDVNKNHDKISGSRVRQAGKSPVWEHLGSWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DCS0</accession>
    <name>A0AAD5DCS0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glutamate decarboxylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_003324</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-glutamate + H(+) = 4-aminobutanoate + CO2</text>
            <dbReference type="Rhea" id="RHEA:17785"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:59888"/>
            <dbReference type="EC" id="4.1.1.15"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the group II decarboxylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000164">
        <property type="protein sequence ID" value="KAI7757192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DCS0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004351">
        <property type="term" value="F:glutamate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006538">
        <property type="term" value="P:L-glutamate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.50:FF:000002">
        <property type="entry name" value="Glutamate decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010107">
        <property type="entry name" value="Glutamate_decarboxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002129">
        <property type="entry name" value="PyrdxlP-dep_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43321">
        <property type="entry name" value="GLUTAMATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43321:SF3">
        <property type="entry name" value="GLUTAMATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00282">
        <property type="entry name" value="Pyridoxal_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="4" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7757192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="106" mass="12180" checksum="84B63F72AD6C9569" modified="2024-05-29" version="1">MVLSRATTSDSDASLNSTFASRYVRTSLPKFKMPEDPIPKEAAYQIINDELMLDGNPRLNLASFVTTWMEPECDKLIMDSINKNYVDMDEYPVTTELQVGYIYRKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DDU2</accession>
    <name>A0AAD5DDU2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_005561</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000100">
        <property type="protein sequence ID" value="KAI7757560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DDU2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43939:SF68">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 290 KDA-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43939">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 158"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="89"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="183"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="250"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="624"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="825"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7757560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="1077" mass="121757" checksum="7A65DE1872F92A14" modified="2024-05-29" version="1" fragment="single">AFGGAEFSDTSVSGKLYYLEEGTSFLLNKYNYLLSGVDMLAHCLAEVRSDFQMENYMETIFPSLQEELVGLKRKELEFNEKTTILEYNHGQLMEQLEKNKETIELLNAEIGKLKGEVELEKTRYSNTKEKLSMAVTKGKALVQQRDSLKQLVAEKTSELERCLVELQEKSTALETAELRNEEIDTLNNKLVETLEIVKSLEDKLASSEKTISLLTEEKSELEIAKCHVEEELHKAMDEISSQTSKFQEALSHEENKISMLMSEKEEAQMEVQKVKEEVSIHASNLDDAKKTIKSLEDSIFQLHTNVSQFSQENEKAVDIKTVLESEIKKLKEEAELEISTLKTELSTCRHELAAVNDKWGPQLSGFFGNLQVLLKDESLFSLFKQSFEKKIESLKNIDRLLKDMKDSFDIEQLQDHPTIEENFQSPTFLPSDLNYDWNTGMIDDEFNAEDTEGIGSYVGKTFDKLNAKNQIIVDQFGNYSTLIDDIIASLLKKLEAIGNTVPLMVQQTVSLQQQVKNVQIDKQMQDNKVVMLENGIKALVSVCADVTKELKFHFENDMLQADPTIGLEKSYPDLLAVDDVAVADVKVAVDELLFAARNVQSVKENMSTTIESLQTELKKTSSMYDKSKEVIEELQVKLEKTKSMYDKEKRAIEELQGELEKTSSMYDKSKELIEELQIKLEKTRSMYDNEKRVIEELQGELEKTRSMYDKVKDENDAFQKRVFKLETELEASKESCNEMSSRLEDYQAKEDKWIERERELSAQTISSVKDHEDDGEKALLSASQIKTLFDKIDGIPIPFRNLVAGDKQAQDSNSVEKLFYIIDNVNELLDQLTLLTHAKEELRSTLSKQALEVDHLKGEFRDGLQNIIKNLGGNESVGARKPADVAGLLLVLEDLVQGLILEGQGSRSELVETQKIAEELSRKVKSMEEFIQNRTGGPDMIQERGVIASTSFPTGSEISEIDDQACVTVGKVGLPLVPSAAQMRSLRKAGSSEQLAITIDSESDRLLDKAETVEDKGHIFKSLHTSGLVPVQGKMIADRLDGLWVSGGQALMRRPRARLGLIAYWLIVHLWLLGTIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DEH2</accession>
    <name>A0AAD5DEH2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Patatin</fullName>
            <ecNumber evidence="6">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_018756</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Lipolytic acyl hydrolase (LAH).</text>
    </comment>
    <comment type="domain">
        <text evidence="6">The nitrogen atoms of the two glycine residues in the GGXR motif define the oxyanion hole, and stabilize the oxyanion that forms during the nucleophilic attack by the catalytic serine during substrate cleavage.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the patatin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="6"/>
    <dbReference type="EMBL" id="JAMZMK010000016">
        <property type="protein sequence ID" value="KAI7758234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DEH2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32241:SF12">
        <property type="entry name" value="OS03G0784100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32241">
        <property type="entry name" value="PATATIN-LIKE PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 6" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="4 6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PNPLA" evidence="8">
        <location>
            <begin position="64"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="409"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7758234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="429" mass="46262" checksum="233A354C4AAEFEBC" modified="2024-05-29" version="1">MATFSLVDNNPSMDKLTSEIFSILENKFLFGYDHNVTPTKDQVSVPIDDMKLHKHMSGKVRVLSIDGGGATDGVFAATSIVHLESSLRRCSGIPDARIADFFDVVTGSGVGGVLAALLFTRGKDGEPLFTADEALRFVVENGHKLSRVGKQGVFRRILGNSSKNGKVFRKMFGDLTLKDTIKAVLIPCYDLTTGAPFVFSRADAVEMEGCDFKMSDVCAATTAVSGPIDTVSVDRRTRISAVTGELATNNPTAMAITHILNNKQEFPFCNGVDDLLVVSLGNGEPSCGVAGNQTPSRAAMLKIVGETVSDTVDQAISMAFGQSRASNYGRIQGNKGFIDSENLNKGKGTNILKLAEHMLRQKNVESVLFQGKKCNKTNLDKLEFFATEIVKETERRKLNILPTVVLRQTTTSSSPRTSSATTLSITSSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-06-18" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G250</accession>
    <name>A0AAD5G250_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TOG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_004725</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011765">
        <property type="protein sequence ID" value="KAI7726054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005881">
        <property type="term" value="C:cytoplasmic microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031110">
        <property type="term" value="P:regulation of microtubule polymerization or depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902903">
        <property type="term" value="P:regulation of supramolecular fiber organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000580">
        <property type="entry name" value="Protein peg1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024395">
        <property type="entry name" value="CLASP_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034085">
        <property type="entry name" value="TOG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048491">
        <property type="entry name" value="XMAP215_CLASP_TOG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21567">
        <property type="entry name" value="CLASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21567:SF9">
        <property type="entry name" value="CLIP-ASSOCIATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12348">
        <property type="entry name" value="CLASP_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21041">
        <property type="entry name" value="XMAP215_CLASP_TOG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01349">
        <property type="entry name" value="TOG"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="TOG" evidence="7">
        <location>
            <begin position="1"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="5">
        <location>
            <begin position="159"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="TOG" evidence="7">
        <location>
            <begin position="282"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="domain" description="TOG" evidence="7">
        <location>
            <begin position="768"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="domain" description="TOG" evidence="7">
        <location>
            <begin position="1134"/>
            <end position="1385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="234"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="503"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="556"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="606"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="667"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="727"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1064"/>
            <end position="1083"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="239"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="523"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="556"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="675"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="690"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="761"/>
            <end position="773"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7726054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="1390" mass="153499" checksum="16C6A4EA144961ED" modified="2024-05-29" version="1">MDEALEMSRSKDTKERMAGVERLHELLEASRKSLTSSEVTSLVDCCLDLLKDNNFRVSQGALQALDSAAVLSGEHLKLHFNSLVPATVDRLGDAKQPVRDAARRLLLTLMQVSSPTLIVERAGXXXXHKSWRVREEFARTVTSAIGLFASTELPLQRAILPSILQMLYDPNPGVREAAILCIEEMYAQIGPQFREELLRHQLPSSMVKDINARLERIEPKQRSADIRVSTYAPAAAAKHSNHNPKRSSPKAKTSSREASLFAGEGDITEKPVDPIKVYSEKELIREFENIASILVPEKDWSLRIGAMQRVEGLVIGGAIDYSCFRGLLKQLVGPLNTQLADRRSSIVKQACHLLNFLSKELLGDFEAFAEMFIPALFKLVVITVLVIAESADNCIKTMLRNCKVARILPRVAENAKHDRSAILRARCCEYALLILEYWTDAPEIHRSADLYEDLIKCCVGDAMSEVRSTARACYRMFSRTWPDRSRRLFSSFDPVIQRIINDEDGGMHRRHASPSLRERNPHTSHNTHNSSSSSSLSGYGTSAIVAMDRNASLPSVASHSSGLFSSQPKSTSKGTERSLESVLHASKQKVSAIESMLRGLDSGRVRSSSLDLGVDPPSSRDPPFPLAAPASNSLTNSLALESTALNGNNRSGGLVLSDIITQIQASRDPAKNAYRGNNGNETLSSYPARRASEKLQERSSFEGHNEIREARRSVNMHTDRHYLDTLYRDSHNSHVPNFQRPLSRKNVPGRSASARRRSFDESQFASGEMSSYTDGPASLSDALSDGLSSGSEWNARVSAFNYIRSLLQQGPKGVQEVIQNFEKVMKFFFQHLDDPHHKVAQAALTTLADIIPTCRKPFESYMERILPHVFSRLIDPKESVRQPCSTTLEIVGKTYSVDSLLLPALLRSLDEQRSPKAKLAVIEFAIVSFNKHAMNSEGAGNSGILKLWLAKLAPLVHDKNTKLKEAAMTCIISVYSHFDSTSVLNYILSLSVEEQNSLRRALKQRTPRIEVDLMNYLQQKKDRQRSKSSYDPYDVVGTSSEEGYVAVSKKTPLFGRYSGSSVDSDGGKKWGSGPTQESIPITGDDNLYQGLDIGSNNGGLASNANDTVASWSVRSEILEGNTNMEVSSTPRLNGLVSSEDQQGGSTPDLELSLSKLASLKISSSPDTKPGIPQILHLISNGGDECSTATKITSLQQLVDISVANDSSIWTKVLDDPDPSIRELALSLIAEMLKNQRDAMEDSIEIVVEKLLHLTKDTTPKVSNEAEHCLTVVLSQYDPFRCLSVIVPLLVTEDEKTLVTCINCLTKLVGRLSQEELMGQLQSFLPALFDAFGNQSADVRKTVVFCLVDMYIMLGKGFLPYLEGLNSTQLRLVTIYANRISQARTGTPIQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G2D3</accession>
    <name>A0AAD5G2D3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_008122</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011711">
        <property type="protein sequence ID" value="KAI7726324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G2D3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071763">
        <property type="term" value="P:nuclear membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33416">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33416:SF18">
        <property type="entry name" value="NUCLEOPORIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="82"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="125"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="197"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="330"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="488"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="347"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="496"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="527"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="559"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="570"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="620"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="630"/>
            <end position="644"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7726324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="710" mass="77051" checksum="1D9403145F4400BB" modified="2024-05-29" version="1">MENSTPSSTAPPYPVGTGAGGKFRKPPRKRPSTPYDRPPPSTTNNNQSHASQDSGGGGWLSKLVVNPARRLIVGGATRILPSFFSKPESLSSDEYEDDSSSDRDDEDVDATVNALGDAKHTFEASLLGVPSSSRSAGPSNEMDKLKGSSEHVNVQQDNLKKTTDNVGLDQIENLLKGKHFSRDESRRLMEILNSRLVDDSNAEGGEKPASVTPQGKGKGNVLDHEIPSTSTIRKQYDLETPMLRLQSNIQDEVAASPIDLAKAYMGSRASELGFNTYGNTSTAGREQQSNDVFPSKPHFFTPSSKSSTCWPGAMVQDQRGYLTPQSQRSRYGLHNFPRTPYSRTKPKLNQLQGDGKSSNISLTTFQQPRWQTKTSSDVMDGGYGSVGPIRRVRNKFASEPQSEGPTRLSSAQLVSSSSASRSFVPVFQKTPVVSSGTSYLHTSDKREQTLKAEANPAGPSNETVRKILEHLDRHKPTPKEKAAELKLATEWKRSPSQDTNLQRSGVSSDSRYLKGPNSGISFRDQTKTSNAETKTSTAVNDAAAPSFGFKNTGAANEKPTVHNDKEKQKSQPWSFDNKINGQDLTRKRPSQPNLKPISFKRPDPQQVVSSDNGRGFTFSFPATTNSASEPPTPSILPSFPPPAAPQSKELPVTPTYSFGKNKSGERVVFSFPSTSSAAVDDGASDLKFNFGSDKKRVSFGLIGSDAIVIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G2V3</accession>
    <name>A0AAD5G2V3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_025184</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011625">
        <property type="protein sequence ID" value="KAI7726610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G2V3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7726610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="86" mass="9499" checksum="835AA484F0E352D2" modified="2024-05-29" version="1">MFLDLMVTTWFSLPNLFKHKETSLLRCRECGFMLFQAWDKGNNCSISSCIAALKTAFGALQQSQETSIASSAADDVEIKGPVFTTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G2V8</accession>
    <name>A0AAD5G2V8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_025199</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011625">
        <property type="protein sequence ID" value="KAI7726625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G2V8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47711">
        <property type="entry name" value="PROTEIN PLASTID TRANSCRIPTIONALLY ACTIVE 16, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47711:SF2">
        <property type="entry name" value="PROTEIN PLASTID TRANSCRIPTIONALLY ACTIVE 16, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13460">
        <property type="entry name" value="NAD_binding_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-binding" evidence="1">
        <location>
            <begin position="94"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7726625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="318" mass="33839" checksum="00BBA50ACF13E1AD" modified="2024-05-29" version="1">MSPVLTANSFILTTSPHSRLTLFKKRNKLVVCAKKVDTSSSAGDVETENSNGFGFSFDFGKVSDVKSLIRVVNNPSTGAVFGKRKDPNTVFVAGATGQAGIRIAQTLLREGFSVRAGVPELGAAQDLARLAVNYKVISTEESKRLNAVESSFQDPEAIAKAIGNATKAVVTIGPGENGPTAEVTTSEALQLIQGAQLAGVSHVAIIYDTSPPSFTSTNNVLDGISSFFNNFFSKSQSLTLKEFIDGLIATDIKYTLIKTKLTDDSASESSYNFVVTAEGTNQETDYKVTKSQIASLVADVFSNTEVAENKVSIYSPSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G4T9</accession>
    <name>A0AAD5G4T9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_033146</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Co-chaperone that regulates diverse cellular pathways, such as programmed cell death and stress responses.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011219">
        <property type="protein sequence ID" value="KAI7728412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G4T9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000774">
        <property type="term" value="F:adenyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.120:FF:000011">
        <property type="entry name" value="BAG family molecular chaperone regulator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000298">
        <property type="entry name" value="BAG family molecular chaperone regulator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.120">
        <property type="entry name" value="BAG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039773">
        <property type="entry name" value="BAG_chaperone_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036533">
        <property type="entry name" value="BAG_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003103">
        <property type="entry name" value="BAG_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12329">
        <property type="entry name" value="BCL2-ASSOCIATED ATHANOGENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12329:SF44">
        <property type="entry name" value="MOLECULAR CHAPERONE REGULATOR BAG-1, UBIQUITIN-RELATED DOMAIN PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02179">
        <property type="entry name" value="BAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00264">
        <property type="entry name" value="BAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63491">
        <property type="entry name" value="BAG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51035">
        <property type="entry name" value="BAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="4">
        <location>
            <begin position="49"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="BAG" evidence="5">
        <location>
            <begin position="142"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="223"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00058673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7728412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="292" mass="32372" checksum="8B978DB010BDFE29" modified="2024-05-29" version="1">MRTRATEMGSIDGNSGAGGVNEWEVRPGGMLVQKRDPDGETNRVPPPMIRVRVKYGSVYHEISINSQATFGELKKMLTGPTGLHHEDQKLMYKDRVRDSKSYLDVVGVKDRSKMVVVEDPISQEKRLLEMRKTAKMEKAAKVISDISFEVDRLAGQVSAIESVISKGGKVAEKTLLNVIELLMNQLLKLDGITVDGDVKLQRKMQVKRIQGYVETLDALKIKNSTSSSNGGMPPGPPQSRRTVVAERHQQEAARATTTTEWETFDSLVAPTSTTKTISTSNTVNPMFNWDLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G505</accession>
    <name>A0AAD5G505_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_010119</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RRM RBM34 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011089">
        <property type="protein sequence ID" value="KAI7729040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G505"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12394">
        <property type="entry name" value="RRM1_RBM34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12395">
        <property type="entry name" value="RRM2_RBM34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034221">
        <property type="entry name" value="RBM34_RRM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4B/4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236:SF25">
        <property type="entry name" value="RNA-BINDING PROTEIN 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="182"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="293"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="371"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="17"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="36"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="54"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="373"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="428"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="449"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7729040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="490" mass="54324" checksum="0F8241B7CA5A1E11" modified="2024-05-29" version="1">MGKKSKSKEQPKPETPNPSSDNIFKSLFGTTHQEPNDPSSSVSIFSPSNPFRTKPQQVQNFLQSDNNIPQNNHTQITNSDNNPLSNKKDKKLLKNIEPEIKKDKISNGVGLESVKSVRSLDFDEEKEGEKKKRKKRVEVEAEYEERKYGGVDLEIKKDEGVKGKIEKVVAKEGFDDEEKLSRTVFVGNLPLKVKKKALLKEFSQFGEIESIRIRSIPLLDDKTPRKGAVIKKKINDAVDRVNAYIVFKSEDYAQASLSHNMAVVGGNHIHVDRACPPRKKLKGENAPLYDSKRTVFIGNLPFDVKDEELYQLFAGFNNLKDCIEAIRVVRDPGTSMGKGIAYVLFSTREAANTVVRKHKLKIRDRELRLSHATKASASTPSKKIESSSADSYNPSKKAAVGGSTSYQGIRATKSGGQKKFATKIAKSGRTESTSEKAVKRKVRSEKRPAVAAKKAAALALKTGGDGGGGGMKRKAKPESNSRNKKPRKFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G5L0</accession>
    <name>A0AAD5G5L0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_019827</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011238">
        <property type="protein sequence ID" value="KAI7728308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G5L0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7728308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="75" mass="8529" checksum="F3B9D30DBB60F51C" modified="2024-05-29" version="1">MFVVGHGAAAAEWWDVQCASDDAAALRMYALMPNEYHWLCLQDDPVPVKLLIFGYILSANPWSFVYMLITFLAIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-10-08" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G6K4</accession>
    <name>A0AAD5G6K4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_010269</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011006">
        <property type="protein sequence ID" value="KAI7729431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001424">
        <property type="entry name" value="Protein kinase superfamily protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47989">
        <property type="entry name" value="OS01G0750732 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47989:SF25">
        <property type="entry name" value="PROLINE-RICH RECEPTOR-LIKE PROTEIN KINASE PERK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="1"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7729431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="157" mass="17639" checksum="85886DB173674EA4" modified="2024-05-29" version="1" fragment="single">MLSRLHHRNLVKLVGYYSNRDNSENLLCYELVPNGSLEAWLHGRLGVNCPLDWDTRMKIALDAARGLAYLHEDSQPSVIHRDFKASNILLENNFHAKVADFGLAKQAPEGRATYXXXXVMGTFGYVAPEYAMTGHLLVKSDVYSYGVVLLELLTGRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G6Y2</accession>
    <name>A0AAD5G6Y2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_024528</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010950">
        <property type="protein sequence ID" value="KAI7729643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G6Y2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7729643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="107" mass="12267" checksum="682E2E12C94737F5" modified="2024-05-29" version="1" fragment="single">MVNREFLKTIDSTSHHNPDGTIKTVLGENQNPRMWIVEFWLTNCTCDKLGTLWRLVSSDQRLSMKVHTAMNPCNHFMVVEVGSDNSRFGCQLSFRVGDLVLEQIINL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G6Z8</accession>
    <name>A0AAD5G6Z8_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_000474</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010467">
        <property type="protein sequence ID" value="KAI7731350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G6Z8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7731350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="102" mass="11477" checksum="44A75D2D6E58DBD6" modified="2024-05-29" version="1">MKNKSSLVTGLMTVSTNQRRIRPGLNIICTQWTEVVGINIWGLKKDNCLHDLKEHTKRITDNAGLVVLFDPKLTRSGTSGSSYFNESILNWGFTRIKGVGWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G7P7</accession>
    <name>A0AAD5G7P7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_000936</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Functions as an E3 ubiquitin ligase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="JAMZMK010010777">
        <property type="protein sequence ID" value="KAI7730466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G7P7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16664">
        <property type="entry name" value="RING-Ubox_PUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000082">
        <property type="entry name" value="RING-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058678">
        <property type="entry name" value="ARM_PUB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057314">
        <property type="entry name" value="PUB2-4-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045210">
        <property type="entry name" value="RING-Ubox_PUB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003613">
        <property type="entry name" value="Ubox_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23315:SF340">
        <property type="entry name" value="RING-TYPE E3 UBIQUITIN TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23315">
        <property type="entry name" value="U BOX DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25598">
        <property type="entry name" value="ARM_PUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25240">
        <property type="entry name" value="PUB2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04564">
        <property type="entry name" value="U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00504">
        <property type="entry name" value="Ubox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51698">
        <property type="entry name" value="U_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="U-box" evidence="10">
        <location>
            <begin position="237"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="8">
        <location>
            <begin position="468"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="8">
        <location>
            <begin position="550"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="8">
        <location>
            <begin position="591"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="343"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="399"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="349"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="401"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7730466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="730" mass="80818" checksum="2E0B73361B54E350" modified="2024-05-29" version="1">MQVGMDMEKVLKCLSNSFSRFIHLVTFGVPTSLPSQQDYKYVANSLKLFKRVLDNIDESCINSDEVLCKEFEELDVAVNEAREFIENWNVHMSKIYGVVQSRHFISKIQRYSIKICETVYRSLESSSSTTSTSILADIQLFTEDLKNLKLEPAAEKLEEALIFIREHKVPPLELIMEIIELLRLSSGHELLKESLAVEKENIKFNQPNQFSYLADLVFHIRETMANLESFKTINGVSVPPYFICPLTLQLMFDPVIVSTGQTYERESIQSWLNNGLLRCPVTRQTISHKNLIPNYTVKALISNWCDENNIKVDRTVDHAEKSSSEDQLVSNLTIENGFDRLSFQQSNHRSTEESCSHSRSESTSTAVSSSEHLCELSVETASDAGVSPRSSNCVNEMVNPNGGNRSYSRNVSLPPDSSLNDTTTFSYVKQLVNDLKSPSNGTQTKAAEEIRYLAKNNMENRILIGQSGAIQPLVSLLHSNQKRTQEHAVTALLNLSINGNIKSMIAEAGAVEPLIHVLETGNTCAKENAAAALFSLSLLEEYRVKIGQSGAIKPLVDLLGSGTLRGKKDAATALFNLSIFHENKARIIQAGAVRHLVELMDPDTQMVDKAVALLSNLASVPEGCLVIAREGGIPLLVEVVETGSQRGKENAASILLQLCLNSPKYCRLVLQEGAVPPLVALSQSGTSRAKEKERFFVILSVKHEDLISKDFNGGDGDMKVKGKCQFQAAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G7W1</accession>
    <name>A0AAD5G7W1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_025798</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010246">
        <property type="protein sequence ID" value="KAI7732400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G7W1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010333">
        <property type="term" value="F:terpene synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016102">
        <property type="term" value="P:diterpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046246">
        <property type="term" value="P:terpene biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00684">
        <property type="entry name" value="Terpene_cyclase_plant_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.600.10:FF:000007">
        <property type="entry name" value="Isoprene synthase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.130">
        <property type="entry name" value="Terpene synthase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034741">
        <property type="entry name" value="Terpene_cyclase-like_1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044814">
        <property type="entry name" value="Terpene_cyclase_plant_C1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001906">
        <property type="entry name" value="Terpene_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036965">
        <property type="entry name" value="Terpene_synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050148">
        <property type="entry name" value="Terpene_synthase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005630">
        <property type="entry name" value="Terpene_synthase_metal-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31225">
        <property type="entry name" value="OS04G0344100 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31225:SF137">
        <property type="entry name" value="TERPENE SYNTHASE 11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01397">
        <property type="entry name" value="Terpene_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03936">
        <property type="entry name" value="Terpene_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01019">
        <property type="entry name" value="Terpene_Cyclase_Like_1_C_Termi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Terpene synthase N-terminal" evidence="3">
        <location>
            <begin position="36"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="Terpene synthase metal-binding" evidence="4">
        <location>
            <begin position="256"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="550"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7732400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="550" mass="63483" checksum="682F372B1A15702C" modified="2024-05-29" version="1" fragment="single">ATLKIFSLKASGKLANVHGYHQSHIKHYDAPYFSITNTHTTPQQVTVNNIIEELKEDTRRALLMTSDPTTTLKLIDAIQRLGIGYYFEEEIENILEKFTQMLPKDDLYTVALYFRLCRHNGLPTNPAEAFQNFMDANGELKRSLSEDIEGLLSLYEASYLGSSGEDFLSHAKEFTIRNLSKSASKLSPKLQRKVLDGLKLPRHMRMERLEARRYIEEYGNEDDHNTIVLEFAKCDYNKVQTVLQGELVKVTRWWEHLGLSKKLGFVRDRHVECFLWTVGLLPNVSFSGSRIELAKAIAILLVIDDIYDTYGTYDDLVLFTNAIQRWDLNEMEQLPDYMKICYEALYNTNNEICDKLMAERGLCVQPFLRKTWVDLTKAYMVEVEWVKTGSIPTLQDYIKTGVTTSGTCMALVHLFFFISNGLTTENVHNLLDPYPNFFTLAGTILRFWDDLGTVKEEQERGDMLSSIHLLMKEKKICEEEGRAEILEIIYGLWNDLNARLVSPDTMFLPIIKVVFNMSRASQVVYEHDDDSYLSSIKNHVQCLFYKHIDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G9B2</accession>
    <name>A0AAD5G9B2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">ascorbate ferrireductase (transmembrane)</fullName>
            <ecNumber evidence="11">7.2.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">M8C21_008965</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">AA19_3_7</strain>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>Fe(3+)(out) + L-ascorbate(in) = monodehydro-L-ascorbate radical(in) + Fe(2+)(out) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:30403"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
            <dbReference type="ChEBI" id="CHEBI:59513"/>
            <dbReference type="EC" id="7.2.1.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.2.1.3" evidence="11"/>
    <dbReference type="EMBL" id="JAMZMK010010350">
        <property type="protein sequence ID" value="KAI7731953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G9B2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140571">
        <property type="term" value="F:transmembrane ascorbate ferrireductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1770:FF:000001">
        <property type="entry name" value="Cytochrome b reductase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043205">
        <property type="entry name" value="CYB561/CYBRD1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006593">
        <property type="entry name" value="Cyt_b561/ferric_Rdtase_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10106">
        <property type="entry name" value="CYTOCHROME B561-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10106:SF22">
        <property type="entry name" value="TRANSMEMBRANE ASCORBATE FERRIREDUCTASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03188">
        <property type="entry name" value="Cytochrom_B561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00665">
        <property type="entry name" value="B561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50939">
        <property type="entry name" value="CYTOCHROME_B561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="4" id="KW-0349">Heme</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="84"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="168"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b561" evidence="14">
        <location>
            <begin position="13"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00051575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAI7731953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="203" mass="22360" checksum="E49F5258C4515714" modified="2024-05-29" version="1">MGLQMKALHLSFVVHVLGVAGAVMVLVWNISFRGGLAWSSSNKNLIFNVHPVLMLIGLVIMGGEAIISYKALPFKKAEKKLIHLILHAIALILGIIGIYMAFKNHNESGIANLYSLHSWLGIGLIVLYGIQWIFGFLMFFYPGGTADLRRESLPWHNSGLDKYGSEAFLVNFTAIVAILYGTFVLLTALSRGPEEDNHSYSAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G9M8</accession>
    <name>A0AAD5G9M8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box/LRR-repeat protein 15-like leucin rich repeat domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_033719</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009970">
        <property type="protein sequence ID" value="KAI7733479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G9M8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000276">
        <property type="entry name" value="F-box/LRR-repeat protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057207">
        <property type="entry name" value="FBXL15_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318:SF272">
        <property type="entry name" value="OS12G0552700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13318">
        <property type="entry name" value="PARTNER OF PAIRED, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25372">
        <property type="entry name" value="DUF7885"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box/LRR-repeat protein 15-like leucin rich repeat" evidence="1">
        <location>
            <begin position="99"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="F-box/LRR-repeat protein 15-like leucin rich repeat" evidence="1">
        <location>
            <begin position="267"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF25372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7733479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="432" mass="47234" checksum="CCDC291489088BCA" modified="2024-05-29" version="1" fragment="single">ISNESLRSISSLEKLEVLLMVGCGLVSDDGLRFLGNGCPSLQVLDVSRCEHVSSAGLISVTRGCIGLRKLNAGYHFLELSSTVFQNLKDLKHLKTIRVDGARVNDSFFQIISSNCMFLAEVGLSKCEGVNDAGVMQLAYGHPGLKVLDLTCCNDLTDMAISAIAHSCTKLLCLKLESCSLLTEKSFSYIGSSCVLLEELDLTECCVNDKGLEYLSKCVELQCLRLGICTDISGKGLSYIASKCKNLKELDLYRCSNVGDDGLGFIASGCKKIRKLNFCYCTKITDIGMAFLSQLEELSDLELRNLIKVTSAGLTALASGCRKLSELDIKHCVNISDAGFWSLGYYSWNLQQINLSFCEVSDVGLCMMMSNLTRLQDVKMVNLTKVSVRGYELALRSSCARLKKVKVLASLRPLLSVELLEALGASGCRIRWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G9Z7</accession>
    <name>A0AAD5G9Z7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Beta-amylase</fullName>
            <ecNumber evidence="3 10">3.2.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_023744</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>Hydrolysis of (1-&gt;4)-alpha-D-glucosidic linkages in polysaccharides so as to remove successive maltose units from the non-reducing ends of the chains.</text>
            <dbReference type="EC" id="3.2.1.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the glycosyl hydrolase 14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.2" evidence="3 10"/>
    <dbReference type="EMBL" id="JAMZMK010010203">
        <property type="protein sequence ID" value="KAI7732598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G9Z7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016161">
        <property type="term" value="F:beta-amylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000066">
        <property type="entry name" value="Beta-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001554">
        <property type="entry name" value="Glyco_hydro_14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018238">
        <property type="entry name" value="Glyco_hydro_14_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001371">
        <property type="entry name" value="Glyco_hydro_14B_pln"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31352">
        <property type="entry name" value="BETA-AMYLASE 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31352:SF40">
        <property type="entry name" value="BETA-AMYLASE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01373">
        <property type="entry name" value="Glyco_hydro_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00750">
        <property type="entry name" value="BETAAMYLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00842">
        <property type="entry name" value="GLHYDLASE14B"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00506">
        <property type="entry name" value="BETA_AMYLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00679">
        <property type="entry name" value="BETA_AMYLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="6 10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 10" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton donor" evidence="8">
        <location>
            <position position="252"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="445"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="360"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="365"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="407"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="446"/>
            <end position="447"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="485"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR601554-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR601554-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7732598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="575" mass="65218" checksum="A8CD77BFBAEC456D" modified="2024-05-29" version="1">MASIVFSKLGSSPTRRSLCRQPDTIQKHVQAVWNQIGDKPMFGSQRIIRRPHALATEVSYNEPKASTPFNENMMANYVPIYVMLQLDIITTDNILKDKVGLEKQLKQLQDAGVDGVMCDVWWGIVESKGPKEYDWSAYKTLFQLVQDCNLKMQVVMSFHQCGGNVGDIVIIPIPQWVRDVGETNPDIFYTNRSGNRNVECLTIGVDNRQLFGGRTAIELYSDYMKSFRENMAEFLDAELFTDIEVGLGPAGELRYPSYPQNQGWAFPGIGEFQCYDKYLKADFEEAATNAGHPEWRLPDDAGEYNDTPDATGFFGSKGYLSDKGKFFLTWYSSKLIMHGDQILDEANKAFLGCKVKLACKISGIHWWYKDDTHAAELTAGYYNLNDRDGYRPIARMLSRHYGVFNFTCLEMRDSEQDAAAKSGPQELVQQVFSAVKREKVLMAGENALERYDRVAYNQILLNVRPNGVSKNGPPKLKMEALTFLRLGDEMLTKKNFRIFKTFVKKLHADQACAADPRTYTTIVPLERSKPEIPIEELLKATEMREPFSFNDETDMSVGGVVSDFIDSLLNMIPFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GAY8</accession>
    <name>A0AAD5GAY8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Mediator of RNA polymerase II transcription subunit 10</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Mediator complex subunit 10</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">MED10</name>
        <name evidence="8" type="ORF">M8C21_028641</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the Mediator complex subunit 10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009897">
        <property type="protein sequence ID" value="KAI7733856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GAY8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019145">
        <property type="entry name" value="Mediator_Med10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13345">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13345:SF14">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 10A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09748">
        <property type="entry name" value="Med10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 6" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="18"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU364146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7733856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="175" mass="19030" checksum="61C47B49C475E6D3" modified="2024-05-29" version="1">MESSQTTGGNGTLNPQNSDASPAGSTGTSTTSSSDDPKQNLNQVINSIQKTLGTIHQLYLTVSSFNVSSQLPLLQRLNALVMEMDNMAKLSDKCNIQIPMEVLNLIDDGKNPDEFTRDVLNSCIAKNQITKGKTDAFKALRKHLLEELEQAFPDEVDDYREIRAASAAVSSVTLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GBC3</accession>
    <name>A0AAD5GBC3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_030496</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10 16">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 16">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010009532">
        <property type="protein sequence ID" value="KAI7735204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GBC3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000211">
        <property type="entry name" value="Integrin-linked protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000355">
        <property type="entry name" value="Integrin-linked protein kinase family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000180">
        <property type="entry name" value="serine/threonine-protein kinase STY46-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF7">
        <property type="entry name" value="OS02G0608500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000654">
        <property type="entry name" value="Integrin-linked_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="repeat" description="ANK" evidence="11">
        <location>
            <begin position="106"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="144"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="29"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="29"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="53"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7735204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="460" mass="52041" checksum="7B78CE9885CA9381" modified="2024-05-29" version="1">MDRAAQLKRGISRQFSTGSMKLSAKFSFKRQTSMDPRSNNVRFRFGRQSSLDPLRRSPTKDPKEELTLPGNLDHTMQLLFVACKGDVAGVKELLDDGVDVNSIDLDGRTALHIAACEGHVEVVQLLLSRKANIDARDRWGSTAAADAKYYGNVETYNILKARGAKTPKTRKTPMAVANPREVPEYELNPLDLQIRKSDGISKARLGPIKLLNGMAQKNAFKYELTLMEKVRHPNVVQFVGAVTQNVPMMIVSEYHSAGDLSAYLQKKGRLSPSKALRFALDIARGMNYLHECKPDPIIHCDLRPKNILLDSSGQLKVAGFGLIRLSKISPDKARLARPVTIDQSNLYIAPEIYKDELFDRGVDVYSFGLILYEMMEGVQPFHPKTPEEAIRLMCVDVMRPPFKTKSKYYPPDLKELIEECWYPDLSIRPKFSEIIIRLDKIVGNCTKNGWWKDTFKLPWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GD29</accession>
    <name>A0AAD5GD29_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_007534</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the plant acyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009143">
        <property type="protein sequence ID" value="KAI7737034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GD29"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.10:FF:000008">
        <property type="entry name" value="Tryptamine hydroxycinnamoyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050317">
        <property type="entry name" value="Plant_Fungal_Acyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31642:SF11">
        <property type="entry name" value="SHIKIMATE O-HYDROXYCINNAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31642">
        <property type="entry name" value="TRICHOTHECENE 3-O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02458">
        <property type="entry name" value="Transferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7737034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="395" mass="44979" checksum="B9F8C3F6A914B491" modified="2024-05-29" version="1">MKVVVIESTMVRPAEATQAKNLWLSSLDLMAPSIHAQQVYFYRPNGALNFFDTEVMKDALSKALVAFYPLGGRLKQGKDGRIEIDCQGQGALFVEAECDGILDDFCNSLQMWWSFANGTSAMHFTNMWSNICRGLDITSPPFMDRTLLRAHDPPQPVFNHIEYHQDQKVNSLGQVPLDETNTVCKMFKVTRKQLNVLKAKSKEDRNTIRYSTFEILASHVWKCVCKSRRLREDVGTKAYILTNGRARLQPQLPPGYFGNVNFITSAIATAGEIQSQPIWYAASKIHDALVTMNNDYLKSALDYLEQHLDQKPIVSYKYTNIRIVSWGRLGIHDADFGWGRPVFMGPLRVRYEGMCFVLPSPIDDGSLSIIIRLEVEQMMLFSNLFYTIKMFESPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GES2</accession>
    <name>A0AAD5GES2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein GAMETE EXPRESSED 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_021086</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008720">
        <property type="protein sequence ID" value="KAI7738789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GES2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040346">
        <property type="entry name" value="GEX1/Brambleberry"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33538">
        <property type="entry name" value="PROTEIN GAMETE EXPRESSED 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33538:SF2">
        <property type="entry name" value="PROTEIN GAMETE EXPRESSED 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="179"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="209"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="234"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="18"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7738789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="346" mass="40458" checksum="BAB3B513547D55D4" modified="2024-05-29" version="1">MELQNGQVSMNVKLDEGMTMLNESANRLGEEMSKLRNEAVEIEKEISKVGDAMIMKMDTLQHKANDIENITETSLDRQKQLLESQRQFHEVLRDVTTFQSQALEESRGTLQQLILLGHSQQQELIQRQEQLKKAHDELVENSKTILAAQETFESKQASMFLAIEKLFNLHNAIFMESRVVKTFLVYSMLIFILYMFTSTKQTYSMRPRLNIWLCVTFLIEFMVLRHGIDIDRQSWIISIVRSTFVILASCQLLYAIYTYRDYETLNHQMLQSLTEKVNGMQRNKQLLYDDDDDDDGDSEVDWSSWVDSDLPEDELEDLDYMLSKEAIVATFSTSGSREYNLRHRRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GEW8</accession>
    <name>A0AAD5GEW8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">40S ribosomal protein S26</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_020070</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS26 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008696">
        <property type="protein sequence ID" value="KAI7738909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GEW8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1740.20">
        <property type="entry name" value="Ribosomal protein S26e"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000892">
        <property type="entry name" value="Ribosomal_eS26"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047864">
        <property type="entry name" value="Ribosomal_eS26_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038551">
        <property type="entry name" value="Ribosomal_eS26_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12538">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12538:SF0">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01283">
        <property type="entry name" value="Ribosomal_S26e"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00733">
        <property type="entry name" value="RIBOSOMAL_S26E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="118"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="118"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7738909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="158" mass="17853" checksum="223A2B11ED1CB8CC" modified="2024-05-29" version="1">MTFKRRNGGRNKHGRGHVKFIRCSNCGKCCPKDKAIKRFLVRNIVEQAAVRDVQEACAFDRKAVGVFFVIKIGLVFSCCQSFDTKFDCFLEYTLPKLYLKIQHCVSCAIHSKVVRVRSRTERRNREPPKRFSHPRDDGPKPGQAPRPGGAGGPPPARP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GF29</accession>
    <name>A0AAD5GF29_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Citrate transporter-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_019394</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008911">
        <property type="protein sequence ID" value="KAI7737996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GF29"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01117">
        <property type="entry name" value="YbiR_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004680">
        <property type="entry name" value="Cit_transptr-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43302:SF15">
        <property type="entry name" value="SILICON EFFLUX TRANSPORTER LSI2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43302">
        <property type="entry name" value="TRANSPORTER ARSB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03600">
        <property type="entry name" value="CitMHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="30"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="287"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="310"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="338"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="379"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="422"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="462"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="domain" description="Citrate transporter-like" evidence="8">
        <location>
            <begin position="2"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7737996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="486" mass="52568" checksum="9C813B4918E46898" modified="2024-05-29" version="1">MPIGRTAGSLLGAMLMVLFQVLTPDQAYESIDLPILGLLFGTMVVSVYLERADMFKYLGKLLSWKSQGSKDLLCRICLISAVSSAFFTNDTSCVVLTEFVLKIARQQNLPPHPFLLALASSANIGSAATPIGNPQNLVIAVQSKIPFGQFLFGIVSAMLKDEEEPPVDIAPEEDVNSHRFSPATMSHLTSENSHDLHAYHEAMNSHSLPSVSGHIDGNIIRNRVVASGDNEIFKDANGTKDVTDNGSLQKKDERAVSRASSMNGFAKEGSDLGLEDIDEKSAKWKLLLWKIGVYAITVGMLVSFLVELNMSWTAITAAMALVVLDFKDARPCLEKVSYSLLVFFCGMFVTVEGFNRTGIPSAIWDFMEPYAEIDKVSGIAILAIVIVVLSNLASNVPTVLLLGSRVAAAAATISPEKEKKAWLILAWVSTVAGNLSLLGSAANLIVCEQARRAQNCGYNLTFWAHLKFGVPSTIIVTAIGLTLIRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GGF0</accession>
    <name>A0AAD5GGF0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Aldehyde dehydrogenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_010749</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008628">
        <property type="protein sequence ID" value="KAI7739406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GGF0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004029">
        <property type="term" value="F:aldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006081">
        <property type="term" value="P:aldehyde metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000003">
        <property type="entry name" value="Aldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012394">
        <property type="entry name" value="Aldehyde_DH_NAD(P)"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43570">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43570:SF17">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE FAMILY 3 MEMBER F1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7739406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="287" mass="32277" checksum="15455533953D01DB" modified="2024-05-29" version="1">MAAAAKNLTPVTLELGGKCPVIFDFLPSSDLKVAAKRIASGKWGACAGQACIGIDYVIVEQKFASTLIEQLKKTIKRFYGDDVKKLKNFSKIVNKNHFDRLRSFLEDPAVSNSIVYGGSFDDTSLIFEPTILLDPPLDAEIMNEEIFGPLLPIITVDNIEDSIDFINSKPKPLALYAFTKNENFKKRILAETSSGSVTFNDTMIQFVCDDLPFGGVGQSGFGRYHGKYSFDTFSHEKAILDRSFYFELEPRHPPWSRFKLEFLRSVYNYNYVGMLLLFLGLKRVSNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GHC6</accession>
    <name>A0AAD5GHC6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_027818</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007934">
        <property type="protein sequence ID" value="KAI7742675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GHC6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000003">
        <property type="entry name" value="Calmodulin A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="189"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="235"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="271"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="310"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="14"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="36"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7742675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="358" mass="40156" checksum="0089256410C9C70C" modified="2024-05-29" version="1">MIIIWVEEEDEISVDEGFNQPGKKSSSKRNNNRSNCLSTTLNPQSRAPEKTRKKNSNSKKSSSNCISKPRVKETTMNRHNANQAYYVLGHKTANIRDLYTLGQKLGQGQFGTTYLCTENSSDPLYVHIVMELCNGGELFDRIIQRGQYSERKAAELTKIIWVVIHIGGKMDGCNGFGLKQVIAESLSEEEIAGLREMFKAMDTDNSGAITFDELKAGLKRFGSTLKDTEIRDLMDAVQLVLTAWCRADVDNSGTIDYGEFVAATMHLNKLDREEHLVSAFRYFDKDGSGYITVDELQQACIDHNMTDILVEDIIREVDQDNDGRIDYGEFVAMMTKGNAGIGRRTMSKSLNNSKRGDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GK58</accession>
    <name>A0AAD5GK58_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein NPGR1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_005543</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007092">
        <property type="protein sequence ID" value="KAI7745915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GK58"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043376">
        <property type="entry name" value="NPG1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44102">
        <property type="entry name" value="PROTEIN NPG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44102:SF4">
        <property type="entry name" value="PROTEIN NPGR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="600"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="16"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7745915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="710" mass="79021" checksum="CAD8DE2D8808D9DA" modified="2024-05-29" version="1">MLCACSGEQFKVEEPISRSPESLATRDFSASGLSSRTGTGDWDSKFEDAQVDEAESTLKEALSLNYEEARALLGRLEYQKGNFDAALQVFYGIDIKSLSPRMIKAISDRTLQHNLKSKRENTVVGVMSLHSVSLLLEAILLKAKSLQEMSRFKEAAKECKMILDIVESALPNGMPDSMPEDCKLQEMFHKALELLPVLWIEAGILNEAIVAYRRALVKPWNLNPQRLSALQKDLAAILLYGGVETTLPQVKTWDSNYPKTNTEEALLLLFILMKKLSCGEIKWDPEIISHLTFALSTCDQFECLAVHLEQLLPRAYKRIERWFFLALCYSAAGQVECALNILNKVSGSSESEHEPHIPSLLLGAKLCSQDPNHAHEGINFARKVIESSNKKTEHFKSIAHKFLGVNYGNAARVSLSDSGRVFYHEESINSLNKAASVDVNDDPEVVFYLGLENAIQRNLDVAFSNMMLYSDMVAGSSPKGFTLLALIVSAEQRFKDAETIVDIALDETGRIGQLELLRLKAVLQIAQEQPKQAIESYKVLLALIQAHDELKNKNIGSEAIERNLEIEAWLDLANVYTKLESLSDAEICVTKAKSKEFYHPGAWHSTGLLFEAKSLHNEALIAFSVALSIEPDYVPSIVSTAETLMKMGGTETLPIARSFLMNSLRLEPTNHDAWFNLALICKKEGFLLQAADFFQAAHELESSAPVQSFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GK97</accession>
    <name>A0AAD5GK97_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_017245</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic initiation factor 4G family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007051">
        <property type="protein sequence ID" value="KAI7746035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GK97"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016281">
        <property type="term" value="C:eukaryotic translation initiation factor 4F complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003890">
        <property type="entry name" value="MIF4G-like_typ-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005735">
        <property type="entry name" value="Znf_LSD1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01053">
        <property type="entry name" value="LSD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23253">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4 GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23253:SF9">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4 GAMMA 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02854">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06943">
        <property type="entry name" value="zf-LSD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zinc finger LSD1-type" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="domain" description="MIF4G" evidence="5">
        <location>
            <begin position="78"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="165"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="165"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF06943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7746035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="188" mass="21113" checksum="3049B0F2669E0E19" modified="2024-05-29" version="1" fragment="single">CSNCRTQLNLPPGAKSIRCPLCQALTPVAAAAESTQPSPFSAGSPFSFSAWYPMLLHKAERKYEVGKVSDAEQAKQRCIRSILNKLTPHNFVKLLYQVKQVKMDNADTLSGLVGQIHDRAVTEPAFVEVYANFCSRLALELPGFGNENDKITFKRILLNKCQEEFERGEREANRSDEEEKNVNRKNHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GML8</accession>
    <name>A0AAD5GML8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Mediator of RNA polymerase II transcription subunit 18</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_000007</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Mediator complex subunit 18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006951">
        <property type="protein sequence ID" value="KAI7746529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GML8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070847">
        <property type="term" value="C:core mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006369">
        <property type="term" value="P:termination of RNA polymerase II transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.320.10:FF:000004">
        <property type="entry name" value="Mediator of RNA polymerase II transcription subunit 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.320.10">
        <property type="entry name" value="Hypothetical Protein Pfu-838710-001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019095">
        <property type="entry name" value="Mediator_Med18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13321:SF2">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13321">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION, SUBUNIT 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7746529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="222" mass="24025" checksum="51133F139E11B9BA" modified="2024-05-29" version="1" fragment="single">KEKCYKLSLDLKQSTNGHVEALEILLLGLCGVPKENLRVHELCLKSGPNLGSEASEIRILCDLMQPEPAWTVRHVGGADQISVLVRTMVESKASKHVLRLFYLLGYKLDHELLRVGFAFHFQRGAQITVTVSSYSRLLKLHATDDDNVPVTPGIQMVEVTAPASAENYTEVAAAVSSFCEYLAPLLHLSKPGVSTGVVPTAAAAAASLMSFSGSTNIRHSEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GMM4</accession>
    <name>A0AAD5GMM4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_010627</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007321">
        <property type="protein sequence ID" value="KAI7745243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GMM4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20654">
        <property type="entry name" value="CYP82"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000026">
        <property type="entry name" value="Cytochrome P450 82C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050651">
        <property type="entry name" value="Plant_Cytochrome_P450_Monoox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47947">
        <property type="entry name" value="CYTOCHROME P450 82C3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47947:SF19">
        <property type="entry name" value="CYTOCHROME P450 82C3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7 8" id="KW-0349">Heme</keyword>
    <keyword evidence="5 7 8" id="KW-0408">Iron</keyword>
    <keyword evidence="3 7 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 8" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="7">
        <location>
            <position position="462"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7745243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="524" mass="59011" checksum="C6779378A0F4B815" modified="2024-05-29" version="1">MNYSFQLVVLSLPILLLFYVIFISQTRSKSKEGAPPEVCGRWPIIGHLHILGGGNKLLYRTLGSMADKYGPAFSIRLGANRAFVVSSWEVAKECFTVNDLALASRPKMAAVKHMCYNYAMFGLGPYTPYWREMRKIVTIELLSNRRIEMLKHIRSLEITCGINELYGRWKENSQGPLVVELTKWLEHTMLNIIMMMVAGKRYVGDGSDGDEAAKCHEAIIDFFRLCGLFVVSDAIPSLSWLDLQGYEKQMKKTGKDLDLVLGGWLEEHRQKRKLGLERNKEDVKDFIDVMLSLEDEGQLPNIEHDSNTIIKSTCLSMILGGSDTMAVTLTWAISLLLNHPDVLKKLQEELDNHVGKDRQVEEADIKNLVYLEAVIKETFRLYPAGPLLAPREAREECTIAGYKVKAGTRLIINVWKLQRDESVWSDPSKFQPERFIGVSHEHVEVSGQQFVLVPFGFGRRSCPGISFSLQVLRLTLARLVHSFDLGQPGGLPVDMTEGPGMTLPKKKPLAVLLTPRLPSSTYAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GMP6</accession>
    <name>A0AAD5GMP6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">OBERON-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_009377</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Probable transcription factor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006522">
        <property type="protein sequence ID" value="KAI7748495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GMP6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010078">
        <property type="term" value="P:maintenance of root meristem identity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010492">
        <property type="term" value="P:maintenance of shoot apical meristem identity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010071">
        <property type="term" value="P:root meristem specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15612">
        <property type="entry name" value="PHD_OBE1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047578">
        <property type="entry name" value="OBE1-like_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004082">
        <property type="entry name" value="OBERON"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032881">
        <property type="entry name" value="Oberon-like_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032535">
        <property type="entry name" value="Oberon_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21736:SF38">
        <property type="entry name" value="PROTEIN OBERON 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21736">
        <property type="entry name" value="VERNALIZATION-INSENSITIVE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16312">
        <property type="entry name" value="Oberon_cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07227">
        <property type="entry name" value="PHD_Oberon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF025218">
        <property type="entry name" value="DUF1423_pln"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01544">
        <property type="entry name" value="ARATH130DUF"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Oberon-like PHD finger" evidence="10">
        <location>
            <begin position="216"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="Oberon coiled-coil region" evidence="11">
        <location>
            <begin position="439"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="80"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="401"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="453"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="410"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR025218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF07227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF16312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7748495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="551" mass="62761" checksum="176F7405618BEDB8" modified="2024-05-29" version="1">MSYSYSHPFSHNASCSMTRNSTENYEVWNGGEGTNGSVHSRFRPVGDGGVALVQGNRSNSENNLSFFPSELPARMKVDTQSFDSRGRGSENTKGLESLDFGRSRKVSRPERILREIVSESVQTMALIIHELPEETFESTKEYLKKLITVQEKRDELVGLQLRLERRSDLTSETLSKANRNQVLLLVSIRMGLESYLSVQNRMPTNELIEVFLYERCKNVNCKRLLPVDDCDCKICSTKKGFCSECMCPVCLNFDFASNTCSWIGCDVCSHWCHAACSIQKNLIKPGQSLKRPAGTTEMLFNCLCCGHASEMFGFIKDVFQSCAVQWGLETLIKELDCVRKIFRGSADFKGQKLHVMAGELITKLQSKEMSPSDVCVTILQFFNTIDTHGSSEFPISTIKVPGSPSKSHHPNQTSLYNTGSSSGMMNTNKMIIEDEWSVKSTKKDAFDSVESLVRIKEAEARMFQNKADEARREAEGYKRMIRLKIEKLDEEYTERISKFNLHETEEQRKKKAEELKVSEDDHCEYYKMKIRMQAEIAGLLERMEKTKQQWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GN39</accession>
    <name>A0AAD5GN39_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GYF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_033846</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006412">
        <property type="protein sequence ID" value="KAI7749067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GN39"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009706">
        <property type="term" value="C:chloroplast inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045037">
        <property type="term" value="P:protein import into chloroplast stroma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025640">
        <property type="entry name" value="GYF_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037471">
        <property type="entry name" value="TIC56"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37755">
        <property type="entry name" value="PROTEIN TIC 56, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37755:SF1">
        <property type="entry name" value="PROTEIN TIC 56, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14237">
        <property type="entry name" value="GYF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GYF" evidence="2">
        <location>
            <begin position="242"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="498"/>
            <end position="522"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7749067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="522" mass="61435" checksum="C0F6A6B3F4171D4E" modified="2024-05-29" version="1">MASINFNPFGENWFKKPPNPLQPINLHNLSESLNPFKPHHSPFAAISLPNPFAKKPDPGPDSNRPKPGTYKKMLEQYYWECENRPDYRHTPEVEKILNEDPIFEKKDNPTREEIEENERWLKEFRENPVVKFLARAEEVLDEINEMELKENSEPYRWEDKKYWKSIPHVIGPDGRPMPRKAIKTKQESDDKFWDFAKQFFFGLWGFRQRPYPPGRPIDVAQAVGYKRLEKRYYDFIMRSSGWYYKDRIGRTRGPCELIQLKTAWGAGIIDKYTFIWGEDMDEWVPIGMIYGMERAVATWEVRLGAAATAFLHKLQRGIPPWVPLKGFEKKSRKQLQDEAYESKNRDLAVLEANDGIWPGVRIPSHALFLWASGSELTSILEQDHMPNKYIPKDMRKQLQKAIPGLRPWEVLSVEQAMDQITYSGEWYREPLGALTTGPPYIRHWNDDVEDFINAYQQFTDLMCDYLEEVIPGFNKVMEKVDAEAEEKYRRRMESAKRKLEARKRALRPQRIDPNADEDDDAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GNJ4</accession>
    <name>A0AAD5GNJ4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">O-methyltransferase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_032956</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006384">
        <property type="protein sequence ID" value="KAI7749350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GNJ4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000057">
        <property type="entry name" value="O-methyltransferase ZRP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016461">
        <property type="entry name" value="COMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001077">
        <property type="entry name" value="COMT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11746">
        <property type="entry name" value="O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00891">
        <property type="entry name" value="Methyltransf_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51683">
        <property type="entry name" value="SAM_OMT_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="O-methyltransferase C-terminal" evidence="4">
        <location>
            <begin position="56"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="O-methyltransferase C-terminal" evidence="4">
        <location>
            <begin position="225"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7749350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="432" mass="48387" checksum="B8B271B6DB4A0318" modified="2024-05-29" version="1" fragment="single">MSMQNDELSKNLLHCQTHIWNHIFSFIKSMSLKCAIQLQIPDIIDRHGSPMLLSELWILHDWSDENCIKILKNCKDAISSKQNGGKLVIIDMVVKVDEGDDELFESQLLFDVLMMTSVSGRERTEKDWAKLFLNGGFSDYKISPILVEALGLKPERTQSIYRLMRMLVHSGFFVKQSVSSTRGYSNEEGEGYSLAPPSRLLLKEDPLSIRPFLLAMLDPLLMDPWQSMTKWFHNDDVTPFHTTHGGSLWDLAGQEPRVNRLFNEAMASDARLVTSVILKHCGGVFQGLDSIVDVGGGTGTVAKAIIEAFPTISCISFDLPHVVNGLVGSKNLTYVGGDMFEALPNANAILLKNCKEAIPCKQNGGKLVIIDMVMKVDQSDDELFQSQLFFDMLMMTLVSGRERTEKEWAKLFLDGGFNDYKITPILGSRSLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GP91</accession>
    <name>A0AAD5GP91_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Inactive poly [ADP-ribose] polymerase RCD1-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_016738</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006739">
        <property type="protein sequence ID" value="KAI7747536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GP91"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003950">
        <property type="term" value="F:NAD+ poly-ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.228.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012317">
        <property type="entry name" value="Poly(ADP-ribose)pol_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044964">
        <property type="entry name" value="RCD1/SRO1-5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022003">
        <property type="entry name" value="RST"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004170">
        <property type="entry name" value="WWE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037197">
        <property type="entry name" value="WWE_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057823">
        <property type="entry name" value="WWE_RCD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32263:SF5">
        <property type="entry name" value="INACTIVE POLY [ADP-RIBOSE] POLYMERASE SRO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32263">
        <property type="entry name" value="INACTIVE POLY [ADP-RIBOSE] POLYMERASE SRO4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12174">
        <property type="entry name" value="RST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23467">
        <property type="entry name" value="WWE_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56399">
        <property type="entry name" value="ADP-ribosylation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117839">
        <property type="entry name" value="WWE domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51059">
        <property type="entry name" value="PARP_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51879">
        <property type="entry name" value="RST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50918">
        <property type="entry name" value="WWE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="WWE" evidence="6">
        <location>
            <begin position="67"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="PARP catalytic" evidence="7">
        <location>
            <begin position="238"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="domain" description="RST" evidence="8">
        <location>
            <begin position="490"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="453"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="453"/>
            <end position="474"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7747536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="560" mass="62825" checksum="F4C4CD3AD747671A" modified="2024-05-29" version="1">MASKWVKVSDSSTRVIFNSKRKRATQIVRASTRALAVRPSPVLNHIRKRKRDESCKSECFSSSSKTLLKNYSNFMKTGVPQRLLFSQDGQWNDFSQDVIDLIREDFLAKKSAIEVKINGRHLVLDILRMIEIDLETGVQKPIAWIDETGSCFFPEIYCSYESHQCNKSETQNDIKFVESESCMTPEVKLNVEIELNGLINNNLEECMDESYVQESNIKRVKLDHNLNNTCKHLDAKADQFVEKIQQSDEDASPCFRKILDPETVRNMFMKGMNPALKADVIDVKKCSGDIMESMLELFEKQVEITQKLRGNANVNYAWFASSTDAPSSSVVCGLGHDGPKLGRYGYGVHLTAVDSAHNSATICDVDEKGVRCMVLCRVILGNMELVLPGSKQFYPSDDCFDSGVDNLDNPNHYVVWNMNMNTHIYPEYAVSFKISPSAEGNIIIAGNGVNLSSRMTTQDPQGPSQRDSSSSKLGNISVEKVPSIGSSTSRAPKSPWMPFSKLFEAISDKVSPDDMKLVHILYESLKGKKTNREEFIRKLRLVVGDQILRSTISSLQSSMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GQN6</accession>
    <name>A0AAD5GQN6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_022422</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005882">
        <property type="protein sequence ID" value="KAI7751510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GQN6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009631">
        <property type="term" value="P:cold acclimation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47877">
        <property type="entry name" value="LATE EMBRYOGENESIS ABUNDANT DOMAIN-CONTAINING PROTEIN / LEA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47877:SF4">
        <property type="entry name" value="LATE EMBRYOGENESIS ABUNDANT PROTEIN ECP63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7751510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="134" mass="14141" checksum="573D56595EF9A925" modified="2024-05-29" version="1">MAASNPDIKTKAGEYIDFTAQKTKEAADFTGQKANEAKDTTIGKAGEFKDFTAQKTKEGADSTVNMATAAKDYTVNTAVAAKDYTFNAAVAAKDYTVGTAVAAKDYTVNTAIAAKDFTVEKVNQANSKDNSESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GR49</accession>
    <name>A0AAD5GR49_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_014669</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006183">
        <property type="protein sequence ID" value="KAI7750234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GR49"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33870">
        <property type="entry name" value="CARDIOMYOPATHY-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33870:SF4">
        <property type="entry name" value="CARDIOMYOPATHY-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="280"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="379"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="289"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="425"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7750234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="448" mass="50749" checksum="F8452CF80AA67AA1" modified="2024-05-29" version="1">MNAEGKNGNSVDNKSSIEEQPNEIREVTPEDHDRLDEKYRKCRLDDYKVKKQECLDGVKDKEKQHDRGKRFVGLTDDEEKSLMDLGISEVERTKRLESLMERRRSRTLSKFQIIRNAMGIGNKSCGQISSLCIPKKNPFLYKSAGDLPDSAPTYMIKNNPFDLPYDPHEEKLILSGDSFQDELMAARQKEPVFCRRESSSLGDIASSESAKDTHEMHFDTEFAIKQLRRLGTSKVAKKEDDNKHPQEKQLITNVVTDENRAIKEGESSEKVVNVVDHTKEPEGDHDVVSSDQSTSSCCSSEDEPILRPNKEAILRCLSMSRMRVMSQGNSFHRNAESFDCGPSALFDKSKTDSFFFGSNKRIHYGSTSSLASDMQVEVSEHDSPRSLNMSSIDEEGKGSLVNSTNPSEMDLNEVILRDIDEESDRLESTMKPEKSNEQDAHCLTEKDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GRD9</accession>
    <name>A0AAD5GRD9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PNPLA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_010894</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006291">
        <property type="protein sequence ID" value="KAI7749726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GRD9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050301">
        <property type="entry name" value="NTE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021771">
        <property type="entry name" value="Triacylglycerol_lipase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14226">
        <property type="entry name" value="NEUROPATHY TARGET ESTERASE/SWISS CHEESE D.MELANOGASTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14226:SF10">
        <property type="entry name" value="TRIACYLGLYCEROL LIPASE 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11815">
        <property type="entry name" value="DUF3336"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 4" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="3 4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PNPLA" evidence="5">
        <location>
            <begin position="236"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="4">
        <location>
            <begin position="267"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="269"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7749726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="497" mass="57215" checksum="71EEB0E2460CB30F" modified="2024-05-29" version="1">MDISSEAAVDRFFIGPSSIFGRTIAFRVLLCKSIGHLRHQIVHVLLFYLHRVRTCVSDVCAPVLSWLHPRNPQGILLLVTLIAFLLKRYTNVKLKAEMAYRRKFWRNMMRAAVTYEEWAHAAKMLDKETPKLNEHDLYDVELVRNKLQELRHRRQECSLRDIIFCMRADLIRNLGNMCNPELHKGRHQVPKLIKEYIDEVSTQLRMVCDSDSDELLLEEKLAFMHETRHAFGRTALLLSGGASLGAFHVGVVKTLVEHKLLPRIIAGSSVGSIMCSVVATRSWPELQSFFDDSWYSLQFFDQMGGIFNVFKRVMTQGALHDIRHLQVLLRNLTNNLTFQEAYDMTGRVLGITVCSPRKHEPPRCLNYLTSPHVVIWSAVTASCAFPGLYEAQELMAKDRSGEIVPYHPPFHWGPEEASGTSVRRWRDGSLEIDLPMIQLKELFNVNHFIVSQANPHIAPLLRFKEFVRAYGGNFAAKVYLCKSVSYYRYSLNLFFIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GSQ5</accession>
    <name>A0AAD5GSQ5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF7477 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_001572</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005788">
        <property type="protein sequence ID" value="KAI7751929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GSQ5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055900">
        <property type="entry name" value="DUF7477"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24289">
        <property type="entry name" value="DUF7477"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7477" evidence="1">
        <location>
            <begin position="1"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF24289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7751929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="134" mass="15415" checksum="4212B3F76D32BFA5" modified="2024-05-29" version="1" fragment="single">TPYSQQSYKVSDSFPFKWINKKWREGFHVTSMATAGARWGVVMSRNAGFSDQVVELDFLYPSEGIHRRWDNGYRITSTAATWDQSALILSVPRRKPGDETQETLRTSQFPSTHVKEKWAKNLYLACICYGRTVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GT46</accession>
    <name>A0AAD5GT46_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">phosphopyruvate hydratase</fullName>
            <ecNumber evidence="3">4.2.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_012598</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="8">Mg(2+) is required for catalysis and for stabilizing the dimer.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the enolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.11" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010004258">
        <property type="protein sequence ID" value="KAI7754180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GT46"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000015">
        <property type="term" value="C:phosphopyruvate hydratase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004634">
        <property type="term" value="F:phosphopyruvate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03313">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.390.10:FF:000001">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.120">
        <property type="entry name" value="Enolase-like C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.10">
        <property type="entry name" value="Enolase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00318">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000941">
        <property type="entry name" value="Enolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036849">
        <property type="entry name" value="Enolase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020810">
        <property type="entry name" value="Enolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020811">
        <property type="entry name" value="Enolase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902:SF45">
        <property type="entry name" value="PHOSPHOPYRUVATE HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00113">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03952">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001400">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00148">
        <property type="entry name" value="ENOLASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00002">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00178">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00001">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01192">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01193">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
        <property type="entry name" value="Enolase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="Enolase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enolase N-terminal" evidence="10">
        <location>
            <begin position="4"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="Enolase C-terminal TIM barrel" evidence="9">
        <location>
            <begin position="147"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="215"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="352"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="250"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="300"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="327"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001400-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001400-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7754180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="397" mass="42780" checksum="5B8607316084A2CA" modified="2024-05-29" version="1">MATIKSVNARQIFDSRGNPTVEVDITLSDGTLARAAVPSGASTGVYEALELRDGGSDYLGKGVSKAVANVNTVIGPAIVGKDPTDQTGIDNYMVQQLDGTKNEWGWCKQKLGANAILAVSLAICKAGASVKNIPLYKHIANLAGNTKLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFKEAMKMGVEVYHNLKAVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKTAIAQAGYTGKVVIGMDVAASEFYGKDETYDLNFKEENNDGSQKISGVELKDLYKSFVSEYPIVSIEDPFDQDDWEHYGKMTAECGDKVQIVGDDLLVTNPTRVQKAINEKTCNALLLKGQIKTGAPCRSERLAKYNQLLRIEEELGSEAVYAGANFRKPVEPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GTR9</accession>
    <name>A0AAD5GTR9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="3">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_022572</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010005885">
        <property type="protein sequence ID" value="KAI7751498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GTR9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000034">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000050">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024729">
        <property type="entry name" value="USP7_ICP0-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029346">
        <property type="entry name" value="USP_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14533">
        <property type="entry name" value="USP7_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12436">
        <property type="entry name" value="USP7_ICP0_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin carboxyl-terminal hydrolase 7 ICP0-binding" evidence="8">
        <location>
            <begin position="54"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin carboxyl-terminal hydrolase C-terminal" evidence="9">
        <location>
            <begin position="303"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="557"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF14533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7751498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="557" mass="65384" checksum="8DED9AAF80A848F7" modified="2024-05-29" version="1" fragment="single">VVREENLREQIGKDIFFDLVDQDKVYSFRVQHQIPSSLFKEEVAKEFGIPVHCQRFWLWTNRKNQTYRPSRPLTPQEEAQPVEVLRKKANNAELKLFLEDSRPVPPPAKTENDILLFFKLYDPSKEELRYVGRLFVKDTGKPKEIVTKLNELAGFAPGQDIQLFEEIKFEPTVICELVDRERTYRANGDIVCFQKRIHVGTVKYRYPNVPSFLEHVHDCQVVRFRPLEKPKKDEFSLELPKLSKYYEIVERVASHLNLDDPSKIRLTSHNCYSQQPKPQPIKYRGVEHLSDILAHYNQTSDILYYEVLDIPLPEMQGLRTLKVAFYQATKDEVVMHTIRLPKQSTVGDVMNNLKTKVELSHKDAELRLLEVFCHKIYKTFPIYEKIENINDQYWTLRAEEIPEEEKDLGPQDRLIHVYHFTKGTSQNLQVQNFGEPFFLVIREGETVAEVKPRIQKKLQVPDEEFSKWKFAFVSLGQVTYLQDSDVVSTCYQKRDCYGDWEQYLGLEHSDKTSKGSYAVNQLEDGDIICFQKRFQAGILNCLYPDVPSFLEHVHDRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GU22</accession>
    <name>A0AAD5GU22_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_015627</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005500">
        <property type="protein sequence ID" value="KAI7753234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GU22"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051026">
        <property type="term" value="P:chiasma assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000405">
        <property type="entry name" value="E3 ubiquitin-protein ligase CCNB1IP1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055328">
        <property type="entry name" value="HEI10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47384">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE CCNB1IP1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47384:SF2">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE CCNB1IP1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14634">
        <property type="entry name" value="zf-RING_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RING-type" evidence="3">
        <location>
            <begin position="2"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="200"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="119"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="200"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7753234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="222" mass="25800" checksum="D280C5E2A19DD1E8" modified="2024-05-29" version="1">MRCNACWRELEGRAISTTCGHILCNEDASKILSNDGACPICDQVLSKSLMRPVDINPSDEWVNMAMAGISPQILMKSSYKSIMFYIGQKELEMQYKMNRIVAQCRQKCETMQEKFTEKMEQVHTAYQKMAKRCQQAEQEIENLSKDKQELQEKFSEKSRQKRKLDEMYDQLRSEYESVKRSAIQPANNFYSRADHDLFANDLPDKKPLTYEEDPSLSLSDCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GVE6</accession>
    <name>A0AAD5GVE6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Calreticulin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_024729</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7 10">Belongs to the calreticulin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005301">
        <property type="protein sequence ID" value="KAI7753716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000018">
        <property type="entry name" value="Calreticulin 1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.250.10">
        <property type="entry name" value="Calreticulin/calnexin, P domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001580">
        <property type="entry name" value="Calret/calnex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018124">
        <property type="entry name" value="Calret/calnex_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009169">
        <property type="entry name" value="Calreticulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009033">
        <property type="entry name" value="Calreticulin/calnexin_P_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11073:SF46">
        <property type="entry name" value="CALRETICULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11073">
        <property type="entry name" value="CALRETICULIN AND CALNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00262">
        <property type="entry name" value="Calreticulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002356">
        <property type="entry name" value="Calreticulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00626">
        <property type="entry name" value="CALRETICULIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63887">
        <property type="entry name" value="P-domain of calnexin/calreticulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00803">
        <property type="entry name" value="CALRETICULIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00804">
        <property type="entry name" value="CALRETICULIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 10" id="KW-0143">Chaperone</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 7 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041767754" description="Calreticulin" evidence="10">
        <location>
            <begin position="26"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="225"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="350"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="245"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="350"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>an alpha-D-glucoside</name>
            <dbReference type="ChEBI" id="CHEBI:22390"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>an alpha-D-glucoside</name>
            <dbReference type="ChEBI" id="CHEBI:22390"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>an alpha-D-glucoside</name>
            <dbReference type="ChEBI" id="CHEBI:22390"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>an alpha-D-glucoside</name>
            <dbReference type="ChEBI" id="CHEBI:22390"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="305"/>
        </location>
        <ligand>
            <name>an alpha-D-glucoside</name>
            <dbReference type="ChEBI" id="CHEBI:22390"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="111"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR002356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR002356-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR002356-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7753716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="409" mass="48040" checksum="CE1492495EF6AC35" modified="2024-05-29" version="1">MRKLKYAAALALVLLVFSICTPSFSEIIFEERFDDGWQSRWVKSDWKRSEGKAGSFKHTAGSWSADPDDKGIQTTTDARHYAISAKIPEFSNKNRTLVLQYSIKFEQDIECGGGYIKLMSGYVNQKKFGGDTPYSVMFGPDLCGTQTKKLHVIVSYQGQNYPIKKDLQCETDKLTHFYTFILRPDASYSVLIDGRERDSGSMYTDWDILPPRKIKDVNAKKPADWEEREYIDDPNQVKPEGYDSIPREIPDPKAKQPDTWDEDEDGKIKNPNYKGKWKIPWIDNPEFEDDPDLYVLKPIKYVGIEVWQVKAGSVFDNVLICDDPEYAKEVVQEVFTHREMEKEALEEAEKIKKAREEEEAKRAREEGERRKRERGYDRRHRDKDYYRDRYRKVXFYKSRQIFMRILFSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GWL6</accession>
    <name>A0AAD5GWL6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_006514</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000018">
        <property type="protein sequence ID" value="KAI7758185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GWL6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="1">
        <location>
            <begin position="13"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7758185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="49" mass="5547" checksum="2A0D4E7A3F27F9EF" modified="2024-05-29" version="1">MLANGAVLHEVTYCQVCDVKCLYKAQYVEHYNGKKHHSKVAHALGQDVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GWT6</accession>
    <name>A0AAD5GWT6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_026041</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Endomembrane system</location>
            <topology evidence="6">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyltransferase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010001697">
        <property type="protein sequence ID" value="KAI7755171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GWT6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053223">
        <property type="entry name" value="Prob_Methyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004159">
        <property type="entry name" value="Put_SAM_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44067:SF5">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44067">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE-DEPENDENT METHYLTRANSFERASE SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03141">
        <property type="entry name" value="Methyltransf_29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="333"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="333"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7755171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="352" mass="38955" checksum="7676670F3BB3230D" modified="2024-05-29" version="1">MVATNILSLYHLSTTMTTTKPPTNPTSPIPDHLIHQLQTIRATITHLTRHPSTTTTTTSTIPPELLLYTQLSSIASSCSHHPNLLHQYMNYTPFATCPPPSSAAAEPLILRGCHPLPRRRCFSLTPNKQPSSLPTNPFSPVPEYSILWNNYNCKSFNCFNSDMGFDLYKERSKLLTYKTELDLPISQLIDIANKSNTVIRLALDIGGGTGTFAAQMKEHNVTVVTTTMSMGAPYNEVAALRGLVALHVPLQQRLPVFDGVLDLVRCGHAVNRWIPVQALEFMVYDVDRVLRGGGLFWVDRFFSKGVDLDKVFGPLIGKLGYKKVKWAVGKKTDSSGVKHGEMALKRNGKPVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GWT7</accession>
    <name>A0AAD5GWT7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA-directed RNA polymerase</fullName>
            <ecNumber evidence="3">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">M8C21_024585</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">AA19_3_7</strain>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RNA polymerase beta chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010003072">
        <property type="protein sequence ID" value="KAI7754543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GWT7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032549">
        <property type="term" value="F:ribonucleoside binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1100.10:FF:000008">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1110.10:FF:000007">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1100.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1110.10">
        <property type="entry name" value="RNA polymerase Rpb2, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015712">
        <property type="entry name" value="DNA-dir_RNA_pol_su2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007644">
        <property type="entry name" value="RNA_pol_bsu_protrusion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007642">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037034">
        <property type="entry name" value="RNA_pol_Rpb2_2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20856">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04563">
        <property type="entry name" value="RNA_pol_Rpb2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04561">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RNA polymerase beta subunit protrusion" evidence="15">
        <location>
            <begin position="31"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="14">
        <location>
            <begin position="210"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF04561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF04563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAI7754543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="432" mass="50200" checksum="AE10369C2CA66D28" modified="2024-05-29" version="1">MSTKSDPRVSSTHRMTPHLPNDPDLKHLKDLFSHHILSFDYFIEHGLEKALMNIKPVQIVDPISDQILILWFEKPELYTPQKERISKQFREALYPFECRQAKVSYTGKFMVDVCFKYGEGPSGPVIREKFTFGQLPIMLKSKLCHLRDADPRKLVSCKEEANEMGGYFILNGLERVVRSLVVPKRNYPTSMARSAFRNRREGFTDKAVVIRCVREDQSAVTLNLYYLSNGSARVGFRIRGRENLLPVGLVLKALIDTTDHEIFLSLTSVYNDKYEKAKGSVGTQILNERAMIILNEVKDLSLYTRTQCLEHIGTYFRQYMKGMENDKCRAVAEAVLRDFVLVHLDNNHDKFNLLIFMVQKLFSFIDQTSIPDNPDSLQTQEILLPGHLVTIYVKERLQEWLLKSKQILEDEIKNRKEKFEFENCMPFFIIDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GX98</accession>
    <name>A0AAD5GX98_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_016679</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in the targeting and/or fusion of transport vesicles to their target membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Prevacuolar compartment membrane</location>
            <topology evidence="8">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Vacuole membrane</location>
            <topology evidence="9">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the synaptobrevin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000976">
        <property type="protein sequence ID" value="KAI7755606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GX98"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14824">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15843">
        <property type="entry name" value="R-SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000004">
        <property type="entry name" value="Vesicle-associated membrane protein 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.50:FF:000008">
        <property type="entry name" value="Vesicle-associated membrane protein 711"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.50">
        <property type="entry name" value="Longin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010908">
        <property type="entry name" value="Longin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001388">
        <property type="entry name" value="Synaptobrevin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051097">
        <property type="entry name" value="Synaptobrevin-like_transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042855">
        <property type="entry name" value="V_SNARE_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21136:SF217">
        <property type="entry name" value="LONGIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21136">
        <property type="entry name" value="SNARE PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13774">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00957">
        <property type="entry name" value="Synaptobrevin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00219">
        <property type="entry name" value="SYNAPTOBREVN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01270">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50859">
        <property type="entry name" value="LONGIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00417">
        <property type="entry name" value="SYNAPTOBREVIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50892">
        <property type="entry name" value="V_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="190"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="Longin" evidence="12">
        <location>
            <begin position="7"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="V-SNARE coiled-coil homology" evidence="13">
        <location>
            <begin position="126"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00060376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00060379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7755606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="220" mass="25171" checksum="5BF94368B4000F5C" modified="2024-05-29" version="1">MTIVYTVVARGSVVLAEYSVTQTNASTIGRQIMEKIGGDDDINVSYLQDRFMFHVKRSDGLTVLCMADDVSGRRVPFTFLEGIHQRFVRTYGRAVLSAQAYGMNDEFSRVLSQQMEYYSNDSNADRINRLKGEMGQVHTVMLQNIDKVLERGDRLELLVDKTATMKNNTLRFKKQSRRYRNAIWWRNVKLMFSLIFLLLVLVYVVVAFACNGLTLPSCLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GZS4</accession>
    <name>A0AAD5GZS4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">MBD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_016044</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000127">
        <property type="protein sequence ID" value="KAI7757408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GZS4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.890.10">
        <property type="entry name" value="Methyl-cpg-binding Protein 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016177">
        <property type="entry name" value="DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037472">
        <property type="entry name" value="MBD8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001739">
        <property type="entry name" value="Methyl_CpG_DNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37701:SF17">
        <property type="entry name" value="METHYL BINDING DOMAIN117"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37701">
        <property type="entry name" value="METHYL-CPG-BINDING DOMAIN-CONTAINING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01429">
        <property type="entry name" value="MBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54171">
        <property type="entry name" value="DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50982">
        <property type="entry name" value="MBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MBD" evidence="6">
        <location>
            <begin position="145"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7757408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="993" mass="109884" checksum="70D323E489C9EF25" modified="2024-05-29" version="1">MLKQLCKSDPNFHDVIDDVNNNINNNSELEGLGLGLGFETLGVKRKRGRPRKNENVVFIRPPASAKRVRCGVGKKEEVVYDEGKDKGLERENGGVVDVVGLGGLEDPYGAEIRRRTNGMTTEDELLGFLRGLNGHWGSRRRKRRVVEACEFGDVLPKGWKLSLCIKKKEGRVWLFCRRYLSPSGRQFESCKEISTYLISVIGEENLDKQNQNVVKINGSDDLALKEGSVNVADLVLQEDIKRDDPIANPSPSSSLPGLPPVPAKCEEQVGLDAMEVQVENDVKTDRIGPRVENVIKKTEPSPAEPDNSDLVLAGEIALVETKDAHSGDNINWCKESSTDELDLELVHDLNIVSHSKQDEHSDIDNSRYMEESCDKLGKNVIMDDEASRKEITEQPPDDTVSQSELFGDELMLSMNDNSNREPETSGEIRVNSVEDKLDSNLDQVIDQENVSEIGWQTGGGGCDGGEDIAVTTTSVGMVDPAVPETIVEKVNICEEKGSHLNMDMDINNTSNELISEKEKVVTDTSSGIFLGRFGLDKDGTTVVKDIDLAKAVNSETSMLFQKQESHGTYDNNNVDKLSSGMTNSRLDELFDVGTDAFGSYEENELNKSLENDVSFSTMEEPVMQINSNKSGEVKLDDFNIFRNNEPKRNESEDVMDFNTKSNLEFCSLVPSENEQAFGFQDCLYDNTMEECKEESSERGLLNHFSDDIFENKMYSTPLDGLKFDEDRDLNSNELSLAFGNSNVPSKIEEAFDVHTNLSMVNRSMVEDLKSGSSSMGGLFNLSGNVKTSSFQNSWEGIKSNEFKFKGSGSKYTTGFGSNHGQTREEVVPSGMWRTGDGNQLQTGLPMSSHGHIPSPSSFHSFNIMPGKAGDGQFRLNERYNEGYGVSGMRSGRPEPVEFSFLTAGRSSQHNPHQLQGDSRVFSYNPEMNQQFDSSFWLGKSTMMPNPASRNQMTGVCALCRNEFHLHPAHFTQDGIASLCPSCSAGMSGHANML</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BM20</accession>
    <name>A0AAD5BM20_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">M8C21_007914</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">AA19_3_7</strain>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010011836">
        <property type="protein sequence ID" value="KAI7725898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BM20"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001023">
        <property type="entry name" value="Os07g0541700 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000217">
        <property type="entry name" value="probable LRR receptor-like serine/threonine-protein kinase At1g53430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.430">
        <property type="entry name" value="Galactose-binding lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051824">
        <property type="entry name" value="LRR_Rcpt-Like_S/T_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021720">
        <property type="entry name" value="Malectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48006">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN DDB_G0281931-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48006:SF81">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11721">
        <property type="entry name" value="Malectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="81"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="137"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAI7725898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="353" mass="38878" checksum="929FE6A16D0563F2" modified="2024-05-29" version="1" fragment="single">MLVEKDFDISDRAGGVGKAIVTTYTVNVTSSLEIRLYWAGKGTINVPTRGTYGPLISAISVDPNYPVPRAKEKGNGVSGGVVAGIVVGAVFSIVLILGVLWWLGYLRRRDSSGLDLSGVELNTRFTLRQIKAATNNFDVANKIGEGGFGPVYKGVLPDGTLIAVKQLSSKSRQGNREFLNELGMISALQHKHLVKLHGCCIEGNQLLLAYEYMENNSLARALFGPEEWQLELDWPTRYKICIGIARGLAFLHEESRLKIVHRDIKATNVLLDKNLDAKISDFGLAKLDEEDETHISTRVAGTYGYMAPEYALRGYLTDKADVYSYGIVLLEIVSGTANTVDRLKENHFILLDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BMB3</accession>
    <name>A0AAD5BMB3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Guanylate kinase 1</fullName>
            <ecNumber evidence="2">2.7.4.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">GMP kinase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_024783</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>GMP + ATP = GDP + ADP</text>
            <dbReference type="Rhea" id="RHEA:20780"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the guanylate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.8" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010011868">
        <property type="protein sequence ID" value="KAI7725744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BMB3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004385">
        <property type="term" value="F:GMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00071">
        <property type="entry name" value="GMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.63.10:FF:000002">
        <property type="entry name" value="Guanylate kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000776">
        <property type="entry name" value="Guanylate kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.63.10">
        <property type="entry name" value="Guanylate Kinase phosphate binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008145">
        <property type="entry name" value="GK/Ca_channel_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008144">
        <property type="entry name" value="Guanylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017665">
        <property type="entry name" value="Guanylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020590">
        <property type="entry name" value="Guanylate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03263">
        <property type="entry name" value="guanyl_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23117:SF24">
        <property type="entry name" value="GUANYLATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23117">
        <property type="entry name" value="GUANYLATE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00072">
        <property type="entry name" value="GuKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00856">
        <property type="entry name" value="GUANYLATE_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50052">
        <property type="entry name" value="GUANYLATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Guanylate kinase-like" evidence="10">
        <location>
            <begin position="29"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00067520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00081967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7725744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="231" mass="25624" checksum="D63F961B3C0D649C" modified="2024-05-29" version="1" fragment="single">VDTQFVQEQKKKFGTEVVCWSKGVIGDAERPIVISGPSGVGKGTLINMLMEEFPAMFGFSVSHTTRAPREKEQNGVHYHFTNRDVMEEEIKAGKFLEFASVHGNLYGTSIEAVDVVADAGKSEFDVESQMRCILDIDVQGARSVRASSLEAIFIFICPPSFEELEKRLRARGTETEELIQKRLNNAKAELELGTSSGLFDHILVNDDLDACYERLKKILGLDGSTDTTLKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BME7</accession>
    <name>A0AAD5BME7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_006600</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011772">
        <property type="protein sequence ID" value="KAI7726035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BME7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37254">
        <property type="entry name" value="OS01G0100500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37254:SF4">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="74"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="139"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="391"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7726035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="600" mass="68077" checksum="23B88CF5EB1C75A5" modified="2024-05-29" version="1">MQCPDTHSFTYNTTLCFCNPGFFYNTTTSTCSPYTVSGTAVDEFDVGSGVDYSINIPANIFAFDTIKKFTQSQAVFLEATLVLLASWLFFCFFVRFGKLGDGRTRWFRIRWWLSRLDVSFATRHWLDDQKVVKKRKTELGGAFSIASWILFIGLFATLLYQVISMRTIEIHSVIATNAPDLASFHNDMEFNITTISTMSCSNFRGFGNLFTGNPAFIDARIAPLSTFVRFACHNTTKGPTITLKCDDCQLISDNLYITWQFVDLPNVPAAAVGFEFKLTARDRRHKKHMSYVSGSLKNGSTNSAKFVTYRGRDPNILQFNLFPRVYRNKHDLRLIQPLFHDFIPGSSFSDANQLRASLQGSNDGFVNTTLHLNYLSSYIVQVDNQSTLGPVGFLADLGGLYCVSIGIFFYLLVQFEYRFKRFRHEDAVMRSIRNSRKAQERWDKLRKYVSFTWGRGLSPYEDRVSMEMACCNCFAVPKGGPPSSFNQRKQTRMDTISFNNKVSTPSDKVLRDVNYQEEHNGNSIMSSSDVDPQVVAAADIPLPPSLEMRAGMEISIPEIQKNLKNLYEYNMLLREKLVSTQSTIHALTSKASASSDQKHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BN12</accession>
    <name>A0AAD5BN12_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tryptophan synthase beta chain-like PALP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_002679</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011982">
        <property type="protein sequence ID" value="KAI7725288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BN12"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7725288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="313" mass="34700" checksum="CF7E35CF31CD751A" modified="2024-05-29" version="1">MAYTLTAITTTTFFPKHHQPPPPPYHHLRSTTITITCSSSPSPSPPQTTTTTNNRQPIPSLLPFSAKYVPFNTVSNESYSLDEIIYRSSSGGLLDVQHDLNALKQFDGKYWRTLFDSRVGRTTWPYGSGVWSKKEWVLPEIDNDDIVSCFEGNSNLFWAERYGKLNLNGMNDLWVKHCGISHTGSFKDLGDPVSIDRAVFALQNSDGIVEEATEEELMDEMAAADATGMFVCPHTGVALVALRKLRERGVIGKGERTVVVSTAHGLKFSQAKIEYHSKAIEGMDCKFANPPVEVKAEYGAVMDVLTKYLSKVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BNS1</accession>
    <name>A0AAD5BNS1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Plastid lipid-associated protein/fibrillin conserved domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_004781</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011532">
        <property type="protein sequence ID" value="KAI7726796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BNS1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009536">
        <property type="term" value="C:plastid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039633">
        <property type="entry name" value="PAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006843">
        <property type="entry name" value="PAP/fibrillin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31906">
        <property type="entry name" value="PLASTID-LIPID-ASSOCIATED PROTEIN 4, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04755">
        <property type="entry name" value="PAP_fibrillin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0934">Plastid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Plastid lipid-associated protein/fibrillin conserved" evidence="4">
        <location>
            <begin position="131"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7726796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="300" mass="34375" checksum="AF488D8407A79E94" modified="2024-05-29" version="1">MAHMISPHLPLTVNTHLHHHHHPSLYSLNNFFPSSSPITHTSIHSRPTTPFFFFNNPLISLTQNNTRTCRSRVSFLQLFQTKSEDLENLKQELLQIIAPLDRGADATPEQQDRVDQGNHHQSVFKWVGLVDKRTRTRQIARKLEAVNKIKEPLKSDLLNGKWELLYTTSQSILQTKVACLTSLFNFEPLENTSGELRPKLLRANGKIYQAINVDTLRAQNMETWPFFNQATANLVPLNTKRVAVKFDAFKVFSLIPIKARGSGRGQLEITYLDEELRISRGNEGNLFILRMVDPSYRVPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BNV6</accession>
    <name>A0AAD5BNV6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_002256</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011592">
        <property type="protein sequence ID" value="KAI7726720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BNV6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="144"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="149"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7726720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="182" mass="19858" checksum="126ED9A1D4B5CFDD" modified="2024-05-29" version="1">MLANLPQLLAYAANNIGATTLLNPSLFDKNLLAQSEAAQIAKFQLLQNILQVLSTTSTTSIPQNIDPLYTMQLSEYLKLNPQLHLQSLEEFAISTSNIPPLSTHAFISPNVDLDHGLGSKAFSEHVSVPMGDVNTDDQLGIIKQSGHLSSSNPTSSSTTHDANWQDFMDDEASGCYWKEIVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BPV1</accession>
    <name>A0AAD5BPV1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_014895</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011406">
        <property type="protein sequence ID" value="KAI7727260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BPV1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7727260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="75" mass="8411" checksum="A077CF66FE1783E4" modified="2024-05-29" version="1" fragment="single">PSTIKMGSNLGVAAASGYASSTMYRGAAKPLENFKRLILSSDCIKANYECTSRGWKRRDFHSDVQYWLQQAYIVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BQM0</accession>
    <name>A0AAD5BQM0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Elongation factor 1-alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_029443</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10">This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011673">
        <property type="protein sequence ID" value="KAI7726424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BQM0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01883">
        <property type="entry name" value="EF1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03693">
        <property type="entry name" value="EF1_alpha_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03705">
        <property type="entry name" value="EF1_alpha_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000003">
        <property type="entry name" value="Elongation factor 1-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000005">
        <property type="entry name" value="Elongation factor 1-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000255">
        <property type="entry name" value="Elongation factor 1-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00118_A">
        <property type="entry name" value="EF_Tu_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054696">
        <property type="entry name" value="GTP-eEF1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050100">
        <property type="entry name" value="TRAFAC_GTPase_members"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004539">
        <property type="entry name" value="Transl_elong_EF1A_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00483">
        <property type="entry name" value="EF-1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008969">
        <property type="entry name" value="PRK12317.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23115">
        <property type="entry name" value="TRANSLATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22594">
        <property type="entry name" value="GTP-eEF1A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 10" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="9 10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="11">
        <location>
            <begin position="55"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7726424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="497" mass="55095" checksum="E711CB1F8FA3926E" modified="2024-05-29" version="1" fragment="single">AIKWSIETPYVVSQLPLNLYIKHPILFCLHPRNVSKFRQRKNPSNKNSFIMGKEKVHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVRQMICCCNKMDATTPKYSKSRYDEIVKEVSSYLKKVGYNPEKIPFVPISGFEGDNMIERSTNLDWYKGPTLLEALDNVSEPKRPSDKPLRLPLQDVYKIGGIGTVPVGRVETGIIKPGMVVTFGPSGLTTEVKSVEMHHEALQEALPGDNVGFNVKNVAVKDLKRGYVASNSKDDPAKGAAGFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFAELQTKIDRRSGKELEKEPKFLKNGDAGIVKMVPTKPMVVETFSEYPPLGRFAVRDMRQTVAVGVIKSVDKKDPTGAKITKAAAKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BTR6</accession>
    <name>A0AAD5BTR6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Alpha-carbonic anhydrase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_020913</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011244">
        <property type="protein sequence ID" value="KAI7728271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BTR6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004089">
        <property type="term" value="F:carbonate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03124">
        <property type="entry name" value="alpha_CA_prokaryotic_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.200.10">
        <property type="entry name" value="Alpha carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041891">
        <property type="entry name" value="Alpha_CA_prokaryot-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001148">
        <property type="entry name" value="CA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036398">
        <property type="entry name" value="CA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023561">
        <property type="entry name" value="Carbonic_anhydrase_a-class"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952:SF208">
        <property type="entry name" value="CARBONIC ANHYDRASE XA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00194">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01057">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51069">
        <property type="entry name" value="Carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51144">
        <property type="entry name" value="ALPHA_CA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041973675" description="Alpha-carbonic anhydrase domain-containing protein" evidence="1">
        <location>
            <begin position="27"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-carbonic anhydrase" evidence="2">
        <location>
            <begin position="33"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7728271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="175" mass="19783" checksum="C8D658A497E95A7C" modified="2024-05-29" version="1">MHTIMVNKNFLFFALFITFCVAFALSQEVDDEPEFLYDVNSPNGPNHWGEINPEWSLCNNGDMQSPIDLTHKRVLTTSKLGRLDIDYKPANTTLINSGHDMALTWIGGAGHIHINGTEYQLNEAHWHTPTEHTINGQRFNLELHLVHQSIDKKNAVVGILYKLGRPDSFLAKVCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BTX1</accession>
    <name>A0AAD5BTX1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_016666</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010975">
        <property type="protein sequence ID" value="KAI7729556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BTX1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32343:SF80">
        <property type="entry name" value="NUCLEOTIDE-BINDING ALPHA-BETA PLAIT DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32343">
        <property type="entry name" value="SERINE/ARGININE-RICH SPLICING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="4"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="217"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="220"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7729556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="271" mass="28416" checksum="2AD98032CFB74C96" modified="2024-05-29" version="1" fragment="single">MSAKTVKVSNLSLKASQRDVSEFFSFSGKIVYVEVQSNDDDCSQNAFVTFTDSQGADTAVLLSGATIIDTTVTVTLAPEYHLPPEAASALPPRGGYTYAPAKGEPGSALSKAEDVVSSLFAKGVVLGKDAVTKAKSFDQQHGITEKVSTGTSIVTEKVKEVDQKLQVSEKAKSAFAAAEQTVSNAGSAIMKNSYVNTSVSWVTSAVDKLVKTASEVGQQTKEKVGKAEDENKQKVVDDYAQGYLTESPKGPGPTESPKGPASAEPKPAVVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BUU7</accession>
    <name>A0AAD5BUU7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">YTH domain-containing family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_029370</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, and regulates mRNA stability. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in mRNA stability and processing.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the YTHDF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010838">
        <property type="protein sequence ID" value="KAI7730113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BUU7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990247">
        <property type="term" value="F:N6-methyladenosine-containing RNA reader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061157">
        <property type="term" value="P:mRNA destabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21134">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.590.10">
        <property type="entry name" value="ph1033 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007275">
        <property type="entry name" value="YTH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045168">
        <property type="entry name" value="YTH_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12357:SF77">
        <property type="entry name" value="YTH DOMAIN-CONTAINING FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12357">
        <property type="entry name" value="YTH YT521-B HOMOLOGY DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04146">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50882">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="YTH" evidence="3">
        <location>
            <begin position="197"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="135"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="358"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="135"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="147"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="172"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="358"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="384"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU369095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7730113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="398" mass="44791" checksum="CB129183B3B8BC57" modified="2024-05-29" version="1">MQIMPPMYNDNSSLLFHPAYGFDPQGAYSPLSPILIDGQLYSPQQFPVSPSYYSQPISPTGFATSAGSGQEGIGENMFWGYVQYPVSNGIGFYNGIGESLSNQSTPVGILGPYEHSFGQQPTPYNMYEASRLNHHGSDKEVKHRDSVNLTNDSNATTIDRNRGPRASKPKGSNSADHASNNNDLYNKPDFVTTYEKAKFFVIKSFSEDNVHRSIKYGVWSSTPLGNRKLDAAYQEAKKDGDTNPVFLFFSVNASGQFCGVAEMIGPVDFENDADHWQLDRWSGQFRVQWHIVKDVPNSRFRHILLENNEHKPVTHSRDSQEVKFEEGIAMLKIFKEHDAGTSILDDFSFYDEREKSFQQTRAKERMSSTKNSVDDASISQLSDRVADSLQLEKQKETQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BUV8</accession>
    <name>A0AAD5BUV8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">START domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_019060</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011166">
        <property type="protein sequence ID" value="KAI7728689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BUV8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34560">
        <property type="entry name" value="POLYKETIDE CYCLASE/DEHYDRASE/LIPID TRANSPORT SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34560:SF1">
        <property type="entry name" value="START DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7728689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="548" mass="62326" checksum="6F56D8E3A50B4A3C" modified="2024-05-29" version="1">MEEKESIIQYRDKLDKTLMSNDLSNMEFLRTLVENQMSKSTQSEDQEVNDNLVKKRTKEVANFISMLRSASGNAVEGSKANDIPHGSWKIKQDTEDCRVMYREGPAGTPLHTLLAEGYVDGPLDVCMCITWEAGLYQKWWPQFHIPAFKITYSECVKKVRMGEQVSLVRMKLSWPLSTREALVHYVTLEYFQEDLIILLLNTIPETENIDKSTHGFTRDGIPGVENVTRIDVVGGVALQKTSSENFDVCRSIANMDVKLDFVPPAIINFISRQLIGSGFKLYKKKVASVTKGDEDFSKALKEPFYGHIREALYSKNKLSNGGLEQKEIKFVDGVLEQKEIKIYDGVLEQKEIKIDDEVLEQETVNIGQPEEHCTTKKQDTDQLPVSEIEEVEEAWLSCEDTDGDDDNNNNISNELFVDGKKEIVIGPEVNQALGTLEKVISIFREFRFSTRSLSLSRSVNNVFTDLEDEKPNDHKLLVNKLTMRNSQESRDSLLSLSSRHKVHEVGLAENNDIKVDVIKNMDKSSLTQKKIKKQRFCCINFTSGRLNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BUZ2</accession>
    <name>A0AAD5BUZ2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">UDP-glycosyltransferase 83A1-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_005591</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011123">
        <property type="protein sequence ID" value="KAI7728886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BUZ2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080043">
        <property type="term" value="F:quercetin 3-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080044">
        <property type="term" value="F:quercetin 7-O-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000061">
        <property type="entry name" value="UDP-glycosyltransferase 83A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000108">
        <property type="entry name" value="UDP-glycosyltransferase 83A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926">
        <property type="entry name" value="GLUCOSYL/GLUCURONOSYL TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11926:SF1412">
        <property type="entry name" value="UDP-GLYCOSYLTRANSFERASE 83A1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7728886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="457" mass="51235" checksum="8AD2AEEF1A2E11CD" modified="2024-05-29" version="1">MKNAHILVIPYPAQGHVMPLMEATRCFTSNGLKVTFVNTEFIHKRLTHACLDIDCLGDLMQMVSVPDGMEPCDDRNEHGKLAETMFQFMPTKLEELIIDINKNDDEKITCIAADCYMGWVLRVAQKMDVRLALFCPSSTAVLAVTMSLKKLMDDEIINKKSGVILKDQKVQLSTSMPLMDPANFVWACMGDPITNQIFFDYLFLEGKEAAEAADHIICNSSMELEPGVFTLFPKMLPIGPLLATNKSARQIGHLWNEDSTCLTWLDQQPACSVIYVAFGSITIFDQLQFEELALGLEDTKMSFLWVVRPGTSDNLDYVFPPDYIDRIGSHAKIVSWAPQQEVLNHPSVACFMSHCGWNSTIEGVSNGVPFLCWPYFADQFVNKTYICDIWKTGVGLNKDETGIVTRGEIKSKVEQLLNNTLKENALNLKEKVADSCVRGGNSSNKNLNTFIEWLKEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BVT3</accession>
    <name>A0AAD5BVT3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_002276</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010693">
        <property type="protein sequence ID" value="KAI7730638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BVT3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7730638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="103" mass="12142" checksum="06A8597B4C95C235" modified="2024-05-29" version="1">MLYLLLEDTFDIDVENSNVSQVIDSYMATTWRGHRDKLHDHIKEIGGSDDLTRAKTTPPSDIMKEDWEYLCDLWSDKKYLEIAKKKVMAHQNENLIVEMVRRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BY19</accession>
    <name>A0AAD5BY19_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">adenine phosphoribosyltransferase</fullName>
            <ecNumber evidence="6">2.4.2.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_027544</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>AMP + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + adenine</text>
            <dbReference type="Rhea" id="RHEA:16609"/>
            <dbReference type="ChEBI" id="CHEBI:16708"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.4.2.7"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the purine/pyrimidine phosphoribosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.7" evidence="6"/>
    <dbReference type="EMBL" id="JAMZMK010010381">
        <property type="protein sequence ID" value="KAI7731806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BY19"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003999">
        <property type="term" value="F:adenine phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006168">
        <property type="term" value="P:adenine salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000022">
        <property type="entry name" value="Adenine phosphoribosyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00004">
        <property type="entry name" value="Aden_phosphoribosyltr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005764">
        <property type="entry name" value="Ade_phspho_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050120">
        <property type="entry name" value="Adenine_PRTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01090">
        <property type="entry name" value="apt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002634">
        <property type="entry name" value="PRK02304.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002636">
        <property type="entry name" value="PRK02304.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11776">
        <property type="entry name" value="ADENINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11776:SF7">
        <property type="entry name" value="PHOSPHORIBOSYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10" id="KW-0660">Purine salvage</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="11">
        <location>
            <begin position="37"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7731806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="179" mass="19368" checksum="FEDB8ED511AC0DA0" modified="2024-05-29" version="1">MSSADRISAIKSSLRVIPNFPKPGIMFQDITTLLLDPKVFKDTVDLFIERYKNENISVVAGIEARGFLFGPSIALAIGAKFVPLRKPKKLPGEVISEKYILEYGSDCLEMHVGAVKSGDRALIVDDLIATGGTVCAAMNLVERAGAEVVECACVIEVPKLKGRERLKSCGIPLYALVES</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BY23</accession>
    <name>A0AAD5BY23_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box/LRR-repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_001326</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010734">
        <property type="protein sequence ID" value="KAI7730549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BY23"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053197">
        <property type="entry name" value="F-box_SCFL_complex_component"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34223:SF101">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34223">
        <property type="entry name" value="OS11G0201299 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7730549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="447" mass="51094" checksum="E5C4851FB9F9483A" modified="2024-05-29" version="1" fragment="single">WLKSILDYAFSHNCQQLTVTCVFPGKLYFPLYLFRSQSLKYLRLTGSVCNFPNAYSMVLASTWELPALTTLHLDYVTFFDNRHAKGIDHFSKCTNLKNLTISHFTMWKLHSGCSHFTVCNPRLCNLTLEYGDCTLDVIQVVAPQLENLTIRDMNSKHLITAPNLTSLLFDCSHLLQFSTDLCSLEKADICISSTDMWNGQTIAALLQRLCNVKFLTLNLELIKPLSSYLNRIALQPSPFANLKSLKIYPVYVHLDDEAHKRVNMSTKVKKYLLDGSPGANFTRISYKEIKEKQRKAQAIENAAAAQNLMENFQVMLEKEKANIGTHIHIDQPMEADAQDQGNAPLEKVQLQFDRKMAQMKSYWEDVRVQIDRGRTKTCDIISQLRDIEVLLLKDLPTSKRDELQACFFSLCAEADTVVKKILHHMKTPQIHFSHCFNELATTSLQSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BYU7</accession>
    <name>A0AAD5BYU7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NusG-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_032249</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010364">
        <property type="protein sequence ID" value="KAI7731885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BYU7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006354">
        <property type="term" value="P:DNA-templated transcription elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031564">
        <property type="term" value="P:transcription antitermination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06091">
        <property type="entry name" value="KOW_NusG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09890">
        <property type="entry name" value="NGN_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.940">
        <property type="entry name" value="NusG, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006645">
        <property type="entry name" value="NGN-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036735">
        <property type="entry name" value="NGN_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043425">
        <property type="entry name" value="NusG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30265:SF4">
        <property type="entry name" value="KOW MOTIF FAMILY PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30265">
        <property type="entry name" value="RHO-INTERACTING TRANSCRIPTION TERMINATION FACTOR NUSG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02357">
        <property type="entry name" value="NusG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00738">
        <property type="entry name" value="NGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82679">
        <property type="entry name" value="N-utilization substance G protein NusG, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0889">Transcription antitermination</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NusG-like N-terminal" evidence="4">
        <location>
            <begin position="94"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7731885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="303" mass="34796" checksum="A04418678521CDB6" modified="2024-05-29" version="1">MMMMNQDLLILSRRPLIHLPFYSPSPIGKPYLLRAALQETLTPKEKREVRHLKRDEKRSYNWREDVEEKLLQKPKKQFNSWKEELNLDKLSELGPQWWILKVSRARGKDTVERMMQALHKNYPDAHFEVFDAAVYEKSKLKNGKISVKPKPLYPGCVFLHCILNRELHNFIREDCEGVGGFVGRMVGNTKRQINKPRPVSKDDMKSIFEEVKEKQEAADKAFEEEHSSSALLSADIKASNKTKGRKRTLLGSAVRIVAGDFVDFSGTVKKLDKKRGLATVGFTLFGKETIADLNLSEIVQAQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BZ87</accession>
    <name>A0AAD5BZ87_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_008494</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010522">
        <property type="protein sequence ID" value="KAI7731179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BZ87"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080147">
        <property type="term" value="P:root hair cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000017">
        <property type="entry name" value="Transcription factor bHLH66"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045843">
        <property type="entry name" value="IND-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16223:SF378">
        <property type="entry name" value="TRANSCRIPTION FACTOR BHLH10-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16223">
        <property type="entry name" value="TRANSCRIPTION FACTOR BHLH83-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="7">
        <location>
            <begin position="44"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="22"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="108"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="193"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="108"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="119"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="135"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="206"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="219"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7731179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="237" mass="23944" checksum="AB3E2404FD25F282" modified="2024-05-29" version="1" fragment="single">AQTFGSPGAAATAVMNQVQAASVTAGGGGAPSQPRQRVRARRGQATDPHSIAERLRRERIAERMKALQELVPNANKTDKASMLDEIIDYVKFLQLQVKVLSMSRLGSAAAGSGSVAPGGAEGGGDCGQGSGGRAGRSSNGAVSSSNNDTMTVTENQVVKLMEEDMGSAMQYLQGKGLCLMPISLATAISTATCNPSGSRNNHPLGGASGGEGGGPSSPNMSVLTVQSANGVSVKDSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C0Y0</accession>
    <name>A0AAD5C0Y0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_017003</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010026">
        <property type="protein sequence ID" value="KAI7733336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C0Y0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7733336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="98" mass="11063" checksum="DAE114FB6A363ABC" modified="2024-05-29" version="1">MTPYRRRRPCLTGGQTPETDAYAGVSAMFTLLFFATASVLYSSHPAHLSFIKWRNMISGCGFMANKSTNHVELHHLFLVETVISRFSKRIQAIPSLLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C1U1</accession>
    <name>A0AAD5C1U1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MalT-like TPR region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_028648</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009897">
        <property type="protein sequence ID" value="KAI7733863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C1U1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:001025">
        <property type="entry name" value="Protein KINESIN LIGHT CHAIN-RELATED 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46284:SF2">
        <property type="entry name" value="PROTEIN KINESIN LIGHT CHAIN-RELATED 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46284">
        <property type="entry name" value="PROTEIN KINESIN LIGHT CHAIN-RELATED 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13374">
        <property type="entry name" value="TPR_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="280"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="490"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="545"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="18"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="56"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="575"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7733863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="575" mass="63342" checksum="8DD6A1670F103B14" modified="2024-05-29" version="1" fragment="single">SVKTPPRITIPDDTTTTNNHQIQSTPDRSNSINPKTTSPAPRRPPSPSPSSSRAKPSPDRTSSKKKSPEKQSLLDESSLDNPDLGPFLLKLARDTIASGDGPTKALDYALRASKSFERCAVEGEPSLDLAMCLHVVAAIYCSLGRFEEAIPVLDRAIKVPELGDTHSMLGQLDRSIECYKEGLKIQMEALGDTDPRVAETCRYLAEAHVQAMQFDEADTLCKRTLEIHRVHSAPASLEEAADRRLMALICEAKGDYESALEHLVLASMAMIANGQDNEVAAIDLSIGNIYLSLSRFDEAVFSYQKALTVFKASKGDNHPSVASVFVRLADLYYKTGKIRESRSYCENALRIYAKPVPGTTPEDIASGMTEISAIYESFNEPEEALKLLQKSMKLLEDKPGQQSTIAGVEARMGVMFYMVGRYEEARGAFESAVAKLRASGERKSAFFGVVLNQMGLACVQLFKIDEAAELFEEAREILEQECGPCHQDTLGVYSNLAATYDALGRVEDAIEILEYVLKLREEKLGTANPDFDDEKKRLAELLKEAGRSRNKKAKSLENLIDPNSKRTKKEASSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C1Y8</accession>
    <name>A0AAD5C1Y8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SBP-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_005324</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009875">
        <property type="protein sequence ID" value="KAI7733938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C1Y8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1100.10">
        <property type="entry name" value="Transcription factor, SBP-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044817">
        <property type="entry name" value="SBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004333">
        <property type="entry name" value="SBP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036893">
        <property type="entry name" value="SBP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31251">
        <property type="entry name" value="SQUAMOSA PROMOTER-BINDING-LIKE PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31251:SF200">
        <property type="entry name" value="TRANSCRIPTION FACTOR SBP FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03110">
        <property type="entry name" value="SBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103612">
        <property type="entry name" value="SBT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51141">
        <property type="entry name" value="ZF_SBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SBP-type" evidence="5">
        <location>
            <begin position="81"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7733938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="347" mass="39242" checksum="A7444DB9340BB974" modified="2024-05-29" version="1">MDWAFTPSSSWDLTELDIDDFDVLPEKNQDMEAFSGSDMLGDGIDGIEGILNGLSEQKHSVMPSSPSCSSKKPRVESSEQQVSCLVDGCRADLSGCRDYHRRHRVCETHSKTPVVTICGKEQRFCQQCSRFHSLGEFDEVKRSCRKRLDGHNRRRRKARPESMYFNYGSFFTSHQGTKLLHFGGSPATTFEDWREKHKLNHKFQVTAEQRTPSDSFAHFCSVRKRRFPFLLGVDSEKVNQSFSKSSFQQVVNTNPSSGHLLVEPKGVLYLLSNSKHTIHNSSLPGPTNNLVQFNGMLSQHSGPHLLSQDVTRPNSGHTNNQLIGMIRFRSEGLLENEAAQVLSFSWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C278</accession>
    <name>A0AAD5C278_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PPIase cyclophilin-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_025004</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010099">
        <property type="protein sequence ID" value="KAI7733013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C278"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.290">
        <property type="entry name" value="Oxygen-evolving enhancer protein 3 (PsbQ), four-helix up-down bundle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044259">
        <property type="entry name" value="CYP37-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048563">
        <property type="entry name" value="CYP38_PsbQ-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023222">
        <property type="entry name" value="PsbQ-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47318">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE CYP37, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47318:SF1">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE CYP37, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21329">
        <property type="entry name" value="CYP38_PsbQ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0793">Thylakoid</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="2">
        <location>
            <begin position="305"/>
            <end position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7733013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="476" mass="51980" checksum="06ED631335453C8D" modified="2024-05-29" version="1">MKIILPAKTCNNLAMTTVPLSSSSYYFSSIISHKPSFNVSRRYCSLTTRSFILCKPKLFQPKLVSCILSKLHHPTECSDQQKTVTKLCMPVLQRGIMRLGAAVSAIVVIIQIISPVPLSRWDASFLPPANAVLYSPDTKVPRTGELALRKAIPANTNMKSIQNSLEELSYLLRIPQRKPYGTMEGNVKKALKIAVDEKESILASLPADQKEKGLALYASLTDEKGEFQTLLRYIKDKDPDRVSVALASSLDSIAQLELLQAPGLSFLLPAQYSNYPRLTGRAVVEFVVEKGDGSMFTPQSGGVPRKAATIQVVLDGYSAPLTTGNFAKLVIDGAYDGMKLKCTDQAILSDSGIDKSIGYSVPLEIMPSGQFEPLYKTTLSIQDGELPVLPLSVYGAVAMAHDDVSEEYSSPNQFFFYLYDKSYSGLGGLSFDEGQFSVFGYTTTGRDVLPQIKTGDVVRSAKLVEGQDNLVLPAEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C2V8</accession>
    <name>A0AAD5C2V8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">K-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_012541</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009905">
        <property type="protein sequence ID" value="KAI7733790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C2V8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002487">
        <property type="entry name" value="TF_Kbox"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01486">
        <property type="entry name" value="K-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51297">
        <property type="entry name" value="K_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="K-box" evidence="2">
        <location>
            <begin position="28"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7733790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="157" mass="18238" checksum="3984121CF22E485A" modified="2024-05-29" version="1">MEETIERYRNHVKEVQPENSSSVEDAQWQHLKDETASMAKKIEMLELAKRKLLGEGLGSSTIEELVQIEKQLERSVSIIRARKMQVYNEQIEELQAKEKLLACQNAMLNGKCLGQTKDKMEEHRATLQSADYEESSDVETELFIGLPEKRMRQDRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C2Y3</accession>
    <name>A0AAD5C2Y3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Rrn7/TAF1B N-terminal cyclin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_024539</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RRN7/TAF1B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009738">
        <property type="protein sequence ID" value="KAI7734386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C2Y3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070860">
        <property type="term" value="C:RNA polymerase I core factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001164">
        <property type="term" value="F:RNA polymerase I core promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042790">
        <property type="term" value="P:nucleolar large rRNA transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048540">
        <property type="entry name" value="Rrn7_cyclin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033599">
        <property type="entry name" value="TAF1B/Rrn7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31576">
        <property type="entry name" value="TATA BOX-BINDING PROTEIN-ASSOCIATED FACTOR RNA POLYMERASE I SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31576:SF2">
        <property type="entry name" value="TATA BOX-BINDING PROTEIN-ASSOCIATED FACTOR RNA POLYMERASE I SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20644">
        <property type="entry name" value="Rrn7_cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Rrn7/TAF1B N-terminal cyclin" evidence="11">
        <location>
            <begin position="127"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="500"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="530"/>
            <end position="549"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF20644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7734386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="661" mass="75327" checksum="E6CF6393B63637EA" modified="2024-05-29" version="1">MANGLSLECQDCGYVGMDDGSDGFFYCQRCGAQADGIRDTAVDNDEMLLTKENLGSVFQRRVTVVKPEPITLSQPLSQFWEKLQQADDAEAGDGVGPTEPADFGTGPRKLGYEDYYSEIRKRYVMGVQIMIELQVKALVEKFNVCPIIVGVIEPVWLRFVASTKLFSDDWADEAINESESQVQGVTEGVKVRAEHKSEPHNLLGKRSVMIWYQAVSKTIPLYFSLVISFLACHLAREPILSTDIIKWTLEGKLPYFAAFVEIDEKIGSPTNACPLSSSRMFRPTKAISMQKLESLAAGIAQEIGLELPPVNFYAIASRYLKQLSLPVNIILPHACRIYEWSMPPEMWFSANEFRLPTRAFVLSILIVSIRILYDIHGFGKWEMSLSCAVKRKNGKGLKVNNDDELSQPSSSHSDVDDSVAVHYTKRPEQSNLDATEILVLLHSKYNELIDTSDHGKDLKEYLKYCKDVVFAGVELSFEDQEEDKIIEDLWNFYHNKEDHKPSELCSLSPNSLHKRPRDDSQSKSNKTKKPKGEITPDPKEPHKDREIRRMKSNMEENKFCYIPPRKRVKRHDYLHYTRKKGDGVYVYAAHADYYILLRSCARVAQLDVRSMHAAVLSFERRLVWLEKNIQQSLKQMPCNEDCQLCHDDNNDNSVNLSKLNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C364</accession>
    <name>A0AAD5C364_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">O-fucosyltransferase family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_031961</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycan metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyltransferase GT106 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009873">
        <property type="protein sequence ID" value="KAI7733955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C364"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006004">
        <property type="term" value="P:fucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11299">
        <property type="entry name" value="O-FucT_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024709">
        <property type="entry name" value="FucosylTrfase_pln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019378">
        <property type="entry name" value="GDP-Fuc_O-FucTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052272">
        <property type="entry name" value="GT106_glycosyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31933">
        <property type="entry name" value="O-FUCOSYLTRANSFERASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31933:SF4">
        <property type="entry name" value="O-FUCOSYLTRANSFERASE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10250">
        <property type="entry name" value="O-FucT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009360">
        <property type="entry name" value="UCP009360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="10" id="KW-0294">Fucose metabolism</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7733955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="454" mass="50978" checksum="EC222480ADD82E35" modified="2024-05-29" version="1" fragment="single">MSSVFESSDVQSGSMDKKEIVSLKFGEGKNVVQMYGRLANMASSALVEVCNAVAVASLLNATLVIPRFLFSNVWKDSSQFGDIYQEDYFIKTLKDEVNIVKDLPLHLKSLDFKEIGSLITDADISKEATPTEYIEKVLPLLSKHGAVHFLGYGNRLGFDPLPSELQRLRCKCNYHALKFVPKIQEMGSLLIRRIRKYDAPRNKLDKQLLGNFIKDFRLNGPDMDRKPLKYIALHLRFEVDMVAYSLCEFGGGQVEKTKLQAYRDVHFPLLMQRLKRSTAVSAEELRRLGKCPLTPEEAALVLAALGFTSETYIYLAGSQIYGGKSRMQPLTNLYPRVITKENLLSPTELAPFKNFSSQLAALDFIACATADVFAITDSGSQLSSLVSGFRTYYGGGHAPTLRPSKKRLAEILSKNQTIDWKEFEARVTKMISGAQSVRLRGWGRSIYRQPRCPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C3F1</accession>
    <name>A0AAD5C3F1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">WAT1-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_018666</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the drug/metabolite transporter (DMT) superfamily. Plant drug/metabolite exporter (P-DME) (TC 2.A.7.4) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009929">
        <property type="protein sequence ID" value="KAI7733688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C3F1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030184">
        <property type="entry name" value="WAT1-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31218">
        <property type="entry name" value="WAT1-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="47"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="81"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="109"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="144"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="193"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="222"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="255"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="287"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="313"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="7">
        <location>
            <begin position="22"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="7">
        <location>
            <begin position="192"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7733688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="380" mass="41643" checksum="611FB17176FAA7CA" modified="2024-05-29" version="1">MNKHVASERMSKFFRKGKPFFAVIFLQFGFAGMDVISKVALNEGMSNYVFVVYRHAVATVAMAPFAMVLDKKIRPKMTKSIFVKLMLLALLEPVIDQNLYFLGMKATTATFAVSMCNVLPAITFIMAFILRLEVVNLKSIRSQAKVVGTITTVAGAMLMTLVKGPLLELFWTKGRTNINVIHNGVDLHHSLKGAIMIIIGCFSWSGFMVLQAITLKSYPAELSLTAWICFLGTIEGAIVALVMERGKAAVWAVKWDTTLLATLYSGVMCSGLAYYIQGIIMKDRGPVFVTAFSPLSMIIVAIMGSIILAEQTYLGRVLGAIVIVAGLYMVVWGKSKDNELSIGEQMSPEKESEENGCHKVITIKSSDGDNQTAEICNSKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C3I7</accession>
    <name>A0AAD5C3I7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Inactive purple acid phosphatase 28</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_014013</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009911">
        <property type="protein sequence ID" value="KAI7733748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C3I7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32440:SF2">
        <property type="entry name" value="INACTIVE PURPLE ACID PHOSPHATASE 28-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32440">
        <property type="entry name" value="PHOSPHATASE DCR2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7733748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="205" mass="23644" checksum="3459C195776542BA" modified="2024-05-29" version="1">MHQSLLLTDNFFKCISNLKSPICSRHEENNACLSFESNLEFHRINLNKQTRAYPDLANRAMIKKNHQSQEIPSLAFFHIPIPEIRSGPFREVFGEYREHVACSIVNSRVMQTLVSMGDVKAVFIGHDHLNDFCGNLNGIWFCYGGGFGYHGYGRAGWPRRGRVILAQLKKGKNEWMGVETIKTWKRLDDENMTKIDQQILWTSPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C492</accession>
    <name>A0AAD5C492_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_020763</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009598">
        <property type="protein sequence ID" value="KAI7734973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C492"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000077">
        <property type="entry name" value="MYB transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000140">
        <property type="entry name" value="Myb transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051953">
        <property type="entry name" value="Plant_SW-associated_TFs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47997">
        <property type="entry name" value="MYB DOMAIN PROTEIN 55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47997:SF73">
        <property type="entry name" value="TRANSCRIPTION FACTOR MYB83-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Myb-like" evidence="9">
        <location>
            <begin position="23"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="10">
        <location>
            <begin position="27"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="10">
        <location>
            <begin position="76"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="9">
        <location>
            <begin position="76"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7734973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="340" mass="38037" checksum="3B9A7924FE5DB8AB" modified="2024-05-29" version="1" fragment="single">PDQSERDQKGCNNNNNVVSKGMPMKTRKGLWSPEEDEKLMNYMLRNGHGCWTDIARNAGLQRCGKSCRLRWINYLRPDLKRGAFSPQEQDLIIHLHSVLGNRWSQIAARLPGRTDNEIKNFWNSTIKKRLKTNKTDASTISQNDSMGDLSADASKDIIGEHGMFMSIHDHEIMNNCMDSSLTSSTTTSSSVNNNLMFMNGHDQFGHLPPLVLDNTNCNSNVGSSFLSISSCLGQGGYGDVLDMPYMFGMENDININPTLGINRVIGENANIATFDNHEVDNKITHHVNHHFNGDSDGNIKVEEEVVGFGNHWAGESFRIGEYDIEGFFANVSSIPYIDDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C5T6</accession>
    <name>A0AAD5C5T6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">P53 and DNA damage-regulated protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_025084</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009694">
        <property type="protein sequence ID" value="KAI7734556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C5T6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030482">
        <property type="entry name" value="PDRG1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21162">
        <property type="entry name" value="P53 AND DNA DAMAGE-REGULATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21162:SF0">
        <property type="entry name" value="P53 AND DNA DAMAGE-REGULATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="5"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7734556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="164" mass="18636" checksum="73AEA59A156B696C" modified="2024-05-29" version="1">MDNTMKQFQEKLIEIEAEAENLLLARHQLVEDDRMRNGNREALTALRKRARTTKTSVSSPFELLMNEIDVSRALVKEICATCGNHDSKEKTWILFSGTDVYARVPFHAAHTILETDQTRLDYDVKKLQSYVKEKTLFISEQGVLADKVGPGVLKSLVTLQDKAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C678</accession>
    <name>A0AAD5C678_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_000527</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009435">
        <property type="protein sequence ID" value="KAI7735765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C678"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000009">
        <property type="entry name" value="PP2A_PR55"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11871">
        <property type="entry name" value="PROTEIN PHOSPHATASE PP2A REGULATORY SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00600">
        <property type="entry name" value="PP2APR55"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7735765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="267" mass="29977" checksum="7482149AF14EBF58" modified="2024-05-29" version="1" fragment="single">DGETFISADDLRINLWNLEISNQSFNIVDVKPANMEDLTEVITSAEFHPTHCNTLAYSSSKGSIRLIDLRQSALCDRHSKLFEEQESPGSRSFFTEIIASISDIKFARDGRYILSRDYMTLKLWDINMDSGPVSTFQVHEYLRPRLCDLYENDSIFDKFECCLSGDGQRIATGSYSNLFRVFGSSPGSTEATTLEASKNPMRRQVQTPSRSLSSSITRVVRRGGESPGNDVNGNSFDFTTKLLHLAWHPTENSIACAAANSLYMYYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C6D8</accession>
    <name>A0AAD5C6D8_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_015648</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009347">
        <property type="protein sequence ID" value="KAI7736171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C6D8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36763">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36763:SF1">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7736171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="165" mass="18962" checksum="103390E8EEB3258E" modified="2024-05-29" version="1">MLSREELLHLFDRFSTLTSQTDVKKRIADAVNDNQEAVAVTTAIQEEILKEMGVDPSYGLSCLGKINMEYENDQDLMISFYRFVANEEMVCEEAELGPDEYAERLQSQQTLHQQQLDMLKHMRNYGADDQSAILEKLRQKMEKDFESEATLLSADEISEIVESRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C6E3</accession>
    <name>A0AAD5C6E3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Serine hydroxymethyltransferase</fullName>
            <ecNumber evidence="10">2.1.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_022364</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the interconversion of serine and glycine.</text>
    </comment>
    <comment type="function">
        <text evidence="10">Interconversion of serine and glycine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + glycine + H2O = (6S)-5,6,7,8-tetrahydrofolate + L-serine</text>
            <dbReference type="Rhea" id="RHEA:15481"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="EC" id="2.1.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 9 10">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 10">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">Belongs to the SHMT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.1" evidence="10"/>
    <dbReference type="EMBL" id="JAMZMK010009398">
        <property type="protein sequence ID" value="KAI7735952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C6E3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004372">
        <property type="term" value="F:glycine hydroxymethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019264">
        <property type="term" value="P:glycine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00378">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000097">
        <property type="entry name" value="Serine hydroxymethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001085">
        <property type="entry name" value="Ser_HO-MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049943">
        <property type="entry name" value="Ser_HO-MeTrfase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019798">
        <property type="entry name" value="Ser_HO-MeTrfase_PLP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039429">
        <property type="entry name" value="SHMT-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000586">
        <property type="entry name" value="PRK00011.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680:SF45">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00464">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000412">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00096">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="7 9 10" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Serine hydroxymethyltransferase-like" evidence="11">
        <location>
            <begin position="25"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="9">
        <location>
            <position position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00059150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000412-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7735952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="394" mass="43626" checksum="DA45D85794D7C497" modified="2024-05-29" version="1">MDPDTESVGKQMKRCRVRAWGNQSLEAADPDIFKIIEKEKHRQYNWIELIASENFVCKAVMEALGSHVTNKYSEGMPGARYYAGNMYIDEIETLCCRRALAAFGVDSESWGVNVQAYSCTSANFAVYTGLLLPGDRIMGLDTPSGGSTSHGYYTPNGMKVSGASIFFESMSYKVTKKTGIIDFEKLEERAVDFRPKILICGGSSYPREWDYSKFRQIADKCGAVLMCDMAQISGLIAAKECASPFEFCDIVTSTTHKSLRGPRGGIIFYRKGPKPRKSGLLLNQGDGGDKYDFEEKINFAVCSALQGGPHNNHIAALAVALKQLATPEYKEYMQQVKKNAQALASALLRRNCRLVTGGTDNHLLLWDLRNLGVTGTNPLFSLYFSLVNLYTSCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C7G5</accession>
    <name>A0AAD5C7G5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">M8C21_001734</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="22">AA19_3_7</strain>
            <tissue evidence="22">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010009326">
        <property type="protein sequence ID" value="KAI7736289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C7G5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000162">
        <property type="entry name" value="Adenine nucleotide alpha hydrolase-like domain kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000146">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase IOS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.430">
        <property type="entry name" value="Galactose-binding lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024788">
        <property type="entry name" value="Malectin-like_Carb-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45631">
        <property type="entry name" value="OS07G0107800 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45631:SF27">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12819">
        <property type="entry name" value="Malectin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="14 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-0675">Receptor</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="495"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="21">
        <location>
            <begin position="570"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="881"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="882"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="598"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="22">
        <location>
            <position position="900"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="KAI7736289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="900" mass="100504" checksum="BB253B715C2BE80D" modified="2024-05-29" version="1" fragment="single">QGERRKIHQDEGLIRLNLTLDDGGTALPEFKIGYMLYCSLHLHSHSTNLFTRSIQCCADSKYTDPNNNITWIPDDKWFSDNRQCQVIVRPLQNGTSFDVTIGITPIGQVNSTDETEIEGNVKATNDYINFCLLPKQGNPYASKIELRPLDLDYGDSSTILKLVHRVDVGNTKGEIRYPQDPHDRIWKPDSDLSQNGNSTLLENRNIIGPNGTKALPPITVLQTARTHSDRLEFWYNFQSGYNDYILNLYFLELDGSVQTGQRVFDIYINDKQRENIDIKSGGSNYKAASINFTANQFLNLSITKANGSRWGPICNAYEILQLRPLLQATDQKDVDVILIIKNELLVENQDNGVLESWTGDPCLPHSWHGVTCNQSNGKTIITKLDLSDGKFKGPLAKGIAMLTHLMELNVSNNNFTHSIPVFPSSSVLTSMDLRNNNFMGRIPDSLTSLPHLTQLYYGCNTDFNKELPTSHNSSRLATDNGMCRGEKTSHPAHGLFIAAIVGAAILVIGILACLICIYKKRRMVNKRSEGKRNTMTKNAIYAIPSSKDDISLKEISIESFTLEQIESAIQHYKTLIGEGGFGSVYRGTLPDGQEVAVKVRSATSTQGTREFNNELTLLSAIRHENLVPLLGFCCDNDQQILVYPFMSNGSLQDRLYGEASKRRALDWPTRLSIALGSARGLSYLHTFSGRSVIHRDVKSSNILLDDSMCPKVADFGFSKYAPQEGDSNASLEVRGTAGYLDPEYYSTQHLSAKSDVFSYGVVLLEIITGREPLNIHRPRNEWSLVEWAKPYIRNSRIEELVDPSIKGGYHTEAMWRVVEVALACIEPFSAYRPSMIDIVRELEDALIIENNASEYLRSLDSFGGSNRFSIDRPIVTLALPTPTASEPSSLLSQPALPQPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C8N4</accession>
    <name>A0AAD5C8N4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_005393</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009150">
        <property type="protein sequence ID" value="KAI7736980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018846">
        <property type="entry name" value="Beta-prop_RSE1/DDB1/CPSF1_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058543">
        <property type="entry name" value="Beta-prop_RSE1/DDB1/CPSF1_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050358">
        <property type="entry name" value="RSE1/DDB1/CFT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004871">
        <property type="entry name" value="RSE1/DDB1/CPSF1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10644">
        <property type="entry name" value="DNA REPAIR/RNA PROCESSING CPSF FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10433">
        <property type="entry name" value="Beta-prop_RSE1_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23726">
        <property type="entry name" value="Beta-prop_RSE1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03178">
        <property type="entry name" value="CPSF_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RSE1/DDB1/CPSF1 first beta-propeller" evidence="4">
        <location>
            <begin position="38"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="domain" description="RSE1/DDB1/CPSF1 second beta-propeller" evidence="5">
        <location>
            <begin position="517"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="domain" description="RSE1/DDB1/CPSF1 C-terminal" evidence="3">
        <location>
            <begin position="926"/>
            <end position="1176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF10433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7736980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="1289" mass="142252" checksum="B73ABA6546966C80" modified="2024-05-29" version="1">MAVSEEESSSASGHNSRLPLAHGASHYLAKRVLKGSAVLQVVHGHIRSASSMDIVFGKETSLELVMVDDQGVLQSICDQPIFGTIKDIAILPWNNRFRRAIPQLHGKDLLLVTSDSGDLSVLSFHNEMHRFFALCHTPICSSPGNLKHQIGRMLTVDSNGCFIATSAYEDHLALFKVTNSTSADIIDKKIFCPSNNEKNASTSMGVSGIHGTIWSMCFISKDMSQLSEEHNPLLAILLNRKGSLLNELLLLEWNTNDNFVHVLSQYAELGPLALDIVEVPYSYGYAFLFRVSDALLMDLRDPHNPLCVYRTSLNFLPSVLDDHNFIEESYRMNDVDEEGNICNVAASALLELKDIVKDDDPMNIDDDNGYSSKSTSNRVCSWCWEPGNFVNPRMIFCVDTGELFMIEIYSESNGLKVNLSDCLYKGLPMKELLWTENGFLIGAAEMADGMVLEFEDGKLNYKSPIQNISPILDMSLVDYHDEKHEQIFACCGMAPEGSLRVIRNGVSLEKLLKTAQDYQGITGTWTIKMNLTDRYHSFLVLSFVEETRVLSVGVSFTDVTDSVGFQSDVCTLACGVISDGVLVQIHQNAVRVALPTAVAHPDGIPFPSPSYTSWSPDNTGISLGAIGNNFIVVATSNPCYLFILGVRQLVGYQYEVYQMQNVRLEYELSCISIPQKSVKEDLSNYPVNYVKIDPTHAVEVGHTFVIGTHRPSVEVVSFKPGHGIKALAVGVISLINSIGTTISGSIPQDVRLVKVDHLYIVSGLRNGMLLRFEWPLPYATSVNSVHSSIIPEVANDSCPVNLELISIRRIGITPAFLIPLNDLLDADIIALSDRPWLLQTARHSLTLNSISFQASTHATPVCSSECPNGLLFVSENSLHLLEMTHNKRLNVQKFHLGGTPRKILYHAESRLLLVLRTDLSDDSCSSDICCVDPMSGSVLSSFKLDLGETGKCMELLKVGSEQILVVGTSLSSGPAIMASGEAESTRGRLIVLCLEHKQNSDSGSMTFYSKGGSSSQRTSPFREISGYGTDSLCSSPDDNSCDETEAWNFRLAYSTNMRGIVLAICPYLDCYFLASAGNAFYVCSFQNDNSLRVKRLAVSRTRFMIMTLTTHFTTIAVGDCRDGVLFYAYNEDSKKVEQLYSDPVQRLVADCRLINIDTAIVSDRMGSIAVLSCSHQSGGELFIWLSSGDEYELLKDVQSRIANHSLTAPILRNSHIEFRSRQSPAGVGNILDGDMLGQFLELSNAEQEDILAVPEDPHAMYRTVRKPQLKHLNVSKVVRLLERLHCAIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C9B9</accession>
    <name>A0AAD5C9B9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Calcineurin-like phosphoesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_020982</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009005">
        <property type="protein sequence ID" value="KAI7737497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C9B9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34211">
        <property type="entry name" value="CALCINEURIN-LIKE METALLO-PHOSPHOESTERASE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34211:SF8">
        <property type="entry name" value="CALCINEURIN-LIKE PHOSPHOESTERASE DOMAIN, APAH TYPE, METALLO-DEPENDENT PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="585"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="2">
        <location>
            <begin position="170"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7737497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="717" mass="83069" checksum="C58A479C398DC290" modified="2024-05-29" version="1" fragment="single">FHGLKYAGLVSHGGVRRSKISPVLQHCTLLWAYGISWSAPVGSAHDDPVLSKPDIYGESSDEISPIYSLWAIWALKYFLSVEESEDNELQFLDMVPWYSGTSADLFKTLFDLLVSVTVFLGRFDMRMMQATMNDSHDGARQEDFLYDHFSEKKEFWFDFMADTCDGGNSSYSVARLLAQPSLDARMNDDLIPLPRGNLLLIGGDLAYPNPSTENYEKRFFYPFEEALQPPSWYNKKHLAVNKPELPIGFFDLRQYDGPQSFVIPGNHDWFDGLQTFMRHICQKSWLGGWFMPQKKSYFALQLPKGWWVFGLDVALHNDIDSFQFKFFSELIRDKVKESDSVIVMTHAPNWILDSYWNNENDETGKNVSHLICDYLNGRCKLRIAGDLHHYMRHSRVQSENPAMVQHLLVNGCGGAFLHPTHVFGNLIKAYNSTYETKAAYPSFQDSRKIALDNILLFRKENWQFDFIGGSIYFILTFSMFPLCKLDHILQDDTISGHTNNFFSAVWDAFMYMVGYHSLYEWYQTVENEYIPDPMVRARIEQWTFGLYPTCIKYLMSAFDVPEVMVVTRTNICKNGMASLSRGSAILYYASVFPYFWVLSTPVASLVFGMYLYICINWLHLHFDEAFSSLRNPDYKSFTRLHIKEDGNLEVFTLAVDKVPKEWNVDSAWNNETEQQHQFSHQRFHPSKWSAILQDPIETVKIVDSFVINKRDEAEMSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CAM1</accession>
    <name>A0AAD5CAM1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Endosulphine</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_013263</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the endosulfine family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008958">
        <property type="protein sequence ID" value="KAI7737768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CAM1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004864">
        <property type="term" value="F:protein phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006760">
        <property type="entry name" value="Endosulphine"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10358">
        <property type="entry name" value="ENDOSULFINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10358:SF43">
        <property type="entry name" value="ENDOSULPHINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04667">
        <property type="entry name" value="Endosulfine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="83"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="129"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="146"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU363120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7737768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="160" mass="17580" checksum="943710CD0FDD08E9" modified="2024-05-29" version="1">MSEINESSKDVNIVDNDPKVEVNDEPSSEEVPNNVEKPEDNPMPSPAQEEAVIKKKYGGLLPRKTPLISKDHDRAFFDSADWALGKGSQKSKGPLEALRPKLQPTPHHQTRSRRSAYAPADEGEVDDGSPNTAASEDQGSELNMTTEDKSHDSEDHSHPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CBG0</accession>
    <name>A0AAD5CBG0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_014519</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008871">
        <property type="protein sequence ID" value="KAI7738110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CBG0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7738110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="142" mass="15862" checksum="D9C8A2932BC4E6A2" modified="2024-05-29" version="1">MPTLTVRHKFGHQDSIFIFVTEPTMAMETPWADEKKSMVVGSATSYDDSDPWTISDHSAAVSIINTKLKNVSADEINQARGLQSLTNHDYMEKIMEVNDKLDMVKDMVRPGCSYEVLNTALRYMSSLATTLSTTPQNVRASL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CD27</accession>
    <name>A0AAD5CD27_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Activator of Hsp90 ATPase AHSA1-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_032590</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AHA1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008673">
        <property type="protein sequence ID" value="KAI7739110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CD27"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.15.10.20">
        <property type="entry name" value="Activator of Hsp90 ATPase Aha1, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036338">
        <property type="entry name" value="Aha1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015310">
        <property type="entry name" value="AHSA1-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13009">
        <property type="entry name" value="HEAT SHOCK PROTEIN 90 HSP90 CO-CHAPERONE AHA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13009:SF22">
        <property type="entry name" value="LD43819P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09229">
        <property type="entry name" value="Aha1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01000">
        <property type="entry name" value="Aha1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103111">
        <property type="entry name" value="Activator of Hsp90 ATPase, Aha1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Activator of Hsp90 ATPase AHSA1-like N-terminal" evidence="2">
        <location>
            <begin position="70"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7739110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="206" mass="23480" checksum="F8F411858EBA4AEA" modified="2024-05-29" version="1">MEGNSIMSTTEKQNTDSTTTSSYTYWVREAKQDIAPLPVHKKLTAEDVSVVSASSTHLGSAWNKAGTWEEKNLNKWATERIKELVLSVGSLDFSSGKAEIAEVSRCTGDAFLVTVRNKKRVGYTYEITLKVKGEWLIGEEKKKFKGNLDLAEFSFGELDDLQAEVRLSEEKDLLHQDKQRILQDMKTFLKPIREKLLEFETELKQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CEE9</accession>
    <name>A0AAD5CEE9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Helicase ATP-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_013929</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA2/NAM7 helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008613">
        <property type="protein sequence ID" value="KAI7739520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CEE9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21407">
        <property type="entry name" value="1B_UPF1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18039">
        <property type="entry name" value="DEXXQc_UPF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000097">
        <property type="entry name" value="Regulator of nonsense transcripts 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.230:FF:000002">
        <property type="entry name" value="regulator of nonsense transcripts 1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045055">
        <property type="entry name" value="DNA2/NAM7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006935">
        <property type="entry name" value="Helicase/UvrB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047187">
        <property type="entry name" value="SF1_C_Upf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040812">
        <property type="entry name" value="UPF1_1B_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887:SF364">
        <property type="entry name" value="REGULATOR OF NONSENSE TRANSCRIPTS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04851">
        <property type="entry name" value="ResIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18141">
        <property type="entry name" value="UPF1_1B_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0347">Helicase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="13">
        <location>
            <begin position="160"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="616"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7739520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="843" mass="92253" checksum="1412D6A422BEEEE0" modified="2024-05-29" version="1" fragment="single">MMKESQSKDNLTIRWDIGLNKKRVAYFVFPKEDNELRLVPGDELRLRYSGDPAHPAWQSVGHVIKLTAQEEVALELRASQGVPVDVNHGFSVDFVWKSTSFDRMQGAMKNFAVDETSVSGYIYHHLLGHEVEMQMVRNALPRRFGAPGLPELNASQVFAVKSVLQRPISLIQGPPGTGKTVTSAALVYHMAKQGQGQVLVCAPSNVAVDQLAEKISATGLKVVRLCAKSREAVSSPVEHLTLHYQVRHLDTSEKSELHKLQQLKDEQGELSSSDEKKYKALKRATEREISQSADVICCTCVGAGDPRLANFRFRQVLIDESTQSTEPECLIPLVLGAKQVVLVGDHCQLGPVIMCKKAARAGLAQSLFERLVLLGVKPIRLQVQYRMHPSLSEFPSNNFYEGTLQNGVTINERQSTGIDFPWPVPNRPMFFYVQMGQEEISASGTSYLNRTEAANVEKIVTTFLRSGVVPSQIGVITPYEGQRAYIVNYMSRNGALRQQLYKDIEVASVDSFQGREKDYIILSCVRSNEHQGIGFLNDPRRLNVALTRARYGIVILGNPKVLSKQPLWNGLLTHYKEHECLVEGPLNNLKQSMVQFQKPKKMYNDRRFFFGGGPGIVPNDNFGSTNPGADRRNSRSRGSYMPPGPPNGAHKPGLHPAGYPMPRVPIPSYHGGPQPYAIPTRGAIHGPFGGVPHVPPPGNRGFGGGRGNAGHIPHQQGSQQPIGAGMGSSFNFPMENPNSQPSPGGPLSQPGYVSNMTQGPSQAFRDGFSVGGMSQDFLGDDFKSQGSHVPYNIADLSTQASQGGYGVDYAAPATQAGFPGSFLNQNSQAGFSRYGAGNDFMSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CEQ1</accession>
    <name>A0AAD5CEQ1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NB-ARC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_023514</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008361">
        <property type="protein sequence ID" value="KAI7740703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CEQ1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11017">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11017:SF307">
        <property type="entry name" value="TIR DOMAIN, P-LOOP CONTAINING NUCLEOSIDE TRIPHOSPHATE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NB-ARC" evidence="2">
        <location>
            <begin position="85"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7740703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="312" mass="35843" checksum="DE83FC3204D64CEC" modified="2024-05-29" version="1">MARRRRCKTLRTRTIMMTGWIRQRLVAKVAATMVVLYGGDVFGGGDDVRPETNFLREIVDTVYNKLDLKEVNLPLNIIGMARHEKEINSWLGQSNLEFLVIYGMGGSGKSTLAKHIYDSNFRKFEYMSFLDLNNDLLKLQEQLLKDISRGTKTKIPCVSRVTRKIEKVLQMNRALIVFDDVTECSQLDALLGTGKINSQSKIIVTTRENTDKWFDQARWRCQKYEMKLLNDEEASQLLCLHAFGSKIPMVGFEEIVQRAIEYCEGNPMALRLLGSSLSMNDDIEIWGSQKVEGLALDMHRLLLEEDFTFKVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CG06</accession>
    <name>A0AAD5CG06_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_002937</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008480">
        <property type="protein sequence ID" value="KAI7740255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CG06"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7740255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="209" mass="23703" checksum="E950967262B296E4" modified="2024-05-29" version="1" fragment="single">MKFGFLMCRLLYMVGMFEVASYAFMVEVKHRAVMCDGGAPVFICWCVSMLIGCYWFPQRLNAFKYIHTAGMISWCNLRDINFDCSGKVHGSLASGWLGVRSNNKGCETGQEETSCIFSTIGASSRPVRFHRLLNLVRRFALCFRLVHLQPSVTFFGPSLQVVSVWLMGANWCCGWMVSVWLMGANSLCKMLECYRITVNVSYSLQTKTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CJ68</accession>
    <name>A0AAD5CJ68_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Dehydrogenase E1 component domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_017771</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008050">
        <property type="protein sequence ID" value="KAI7742215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CJ68"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016624">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009083">
        <property type="term" value="P:branched-chain amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050771">
        <property type="entry name" value="Alpha-ketoacid_DH_E1_comp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001017">
        <property type="entry name" value="DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43380:SF11">
        <property type="entry name" value="2-OXOISOVALERATE DEHYDROGENASE SUBUNIT ALPHA 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43380">
        <property type="entry name" value="2-OXOISOVALERATE DEHYDROGENASE SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00676">
        <property type="entry name" value="E1_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dehydrogenase E1 component" evidence="2">
        <location>
            <begin position="162"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7742215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="211" mass="23575" checksum="D07562EE8183EF6C" modified="2024-05-29" version="1" fragment="single">MNMVLWLTRSRRGGGNLMKNIICSNFVTLCPNLQTSTHSSSSSPTTLCDSINGKGTSAFPYSYSYSFSSTYASFWGTRHFNSMQAGEHMLSEEDDNDGSSFGKTQVMDFAGGKIKYTTEIKFLPSSSEERVQCFRILDEDGYPISSNMMEHMYKELALKVYRGMVTLNTMDKFLYEAQRQGRLSFYLTSFGEEAINLASAAALSIDDIVLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CJJ6</accession>
    <name>A0AAD5CJJ6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UspA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_007656</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008146">
        <property type="protein sequence ID" value="KAI7741694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CJJ6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd23659">
        <property type="entry name" value="USP_At3g01520-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31964">
        <property type="entry name" value="ADENINE NUCLEOTIDE ALPHA HYDROLASES-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31964:SF113">
        <property type="entry name" value="USPA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="1">
        <location>
            <begin position="7"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7741694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="158" mass="17618" checksum="841945B0C69E1188" modified="2024-05-29" version="1">MEDQKNMKKVMVAIDQSEYSRYALEWALQNLHESIANNQLFIYTVQPISDYSYLHASAYGATPPELIRNIQENQKKVALSLLDQAKDLCNKYGITAETITEVGDPKDLICEAVEKLKVQLLVMGSSSRGALKRAFLGSVSNHCVQNVKCPVLVVKKTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CR59</accession>
    <name>A0AAD5CR59_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Suppressor of RPS4-RLD 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_003898</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007395">
        <property type="protein sequence ID" value="KAI7744996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CR59"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044650">
        <property type="entry name" value="SRFR1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44749">
        <property type="entry name" value="SUPPRESSOR OF RPS4-RLD 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44749:SF1">
        <property type="entry name" value="TETRATRICOPEPTIDE-LIKE HELICAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12895">
        <property type="entry name" value="ANAPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="229"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="263"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="297"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="365"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="399"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="467"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="65"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="191"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="70"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="90"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="111"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="134"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7744996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="792" mass="88597" checksum="9F055F37F67B6B4B" modified="2024-05-29" version="1">MLSMPRTPLAIGDRKIQFRRAYSALGRKDDAILVWEQGYEYAVRQSTDLKQLLELEELLTAVKKSTAASNDKDAKQLSERSVSKPEPTDSGKSSETCNTHTISSLGSEPCVDSREVLQIEGKPKDVSYMPNGSGKKDKVEDSKSFHMPNGNHEKQKNGTCNISIDFGARSEAHSSTSDNRNKSDLISNISLIPSKSSDIPEMPNEQSNDAKGNKKFSVTRISKAKSINVDFRLSRGIAQVNEGQYARAISIFDQILREDPTYPEALIGRGTAYAFKRELDSAIADFTKAIQSNPSAGEAWKRRGQARAALGESSEAIADLTKSLEFEPDSADILHERGIVNFKLKDFHAATEDLSACVKLDKDNKSAYTYLGLALSSVGEYKKAEEAHMKAIQIDEKFLEAWGHLAQLYQDLANSTKALDCLERVLQIDPRFAKGYHLRGLLLHAMGDHRNAIKDLTNGLNIESSNIESLYLRASCHHAVGEYKEAVKDYDAALDLELDSMDKFVLQCLAFYQKEIALYTSAKVNSEFCWFDIDGDVDPLFKEYWCKRLHPKNVCEKVFRQPPLRKAKLKKQDFILTKPKIALLLAADRIGKTIQYHCQGFLPNRRQHRMAGLAAVEIAQKVSKIWRSLQSEWRGERGFDFAIRTPCTPARWDDFDEEMTSSWEVLCNAYCGETYGSNDFGTLEIVREAILRMTYYWHNFMPLSRGSAVVGFSVMLGLLLAANMDFTGSIPEGRQVDWDAILSLDPNSFMDSVKSWLYPSLKVTPTLKDIPDVASTLETTGSVVAALSSYSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CS17</accession>
    <name>A0AAD5CS17_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UTP25 NTP hydrolase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_010206</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006939">
        <property type="protein sequence ID" value="KAI7746602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CS17"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034511">
        <property type="term" value="F:U3 snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010678">
        <property type="entry name" value="UTP25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053940">
        <property type="entry name" value="UTP25_NTPase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12933">
        <property type="entry name" value="ORF PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12933:SF0">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 25 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22916">
        <property type="entry name" value="UTP25_NTPase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UTP25 NTP hydrolase-like" evidence="2">
        <location>
            <begin position="2"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="91"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7746602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="137" mass="15734" checksum="FA8402D74E7AB7C4" modified="2024-05-29" version="1" fragment="single">MDAYLLHSLNHIFKTRDLVTKNDKTLAKQKESKGEEVLNSEKFLDQGFTRPKVLIILPIRSIAYRVINRLIQLTPEGHKVNVDHADRFCDEFGSGGGDDQEDEDDRSKSWKKSKPLDHQALFRGNSNDDFMIGIKFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CS31</accession>
    <name>A0AAD5CS31_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_021181</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the chaperonin (HSP60) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007228">
        <property type="protein sequence ID" value="KAI7745491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CS31"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001844">
        <property type="entry name" value="Cpn60/GroEL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45633">
        <property type="entry name" value="60 KDA HEAT SHOCK PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00298">
        <property type="entry name" value="CHAPERONIN60"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="196"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7745491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="350" mass="37624" checksum="B496E8568C56D210" modified="2024-05-29" version="1">MASTFAGVTAVGSLASSSNRLMDKNISTSSNKLSSFASISSSSLGRKQNLALKRAPHSSQIKVTAKELYFNNDGSAIKKLQVSHRICLSLSCWGYSWSKGRNVVLQSKYGAPKIVNDGVTVAKEVDLEDPVENIGAKLVRQAAAKTNDLAGDGTTTSVVLAQALITEGVKVVAAGANPALITRGIEKTTKALVAELKLISKEIAEQEYEKEKLNERIAKLSSGVAVIQRNSAIHCRAKQTQAFMALNGLMALKNLIALIELMPVSGPTAVIGRMAVGGHLEAGMMCCFGMFVDVAAALQESSKDFWIMDMKIIWVGVKLPMFSVPEALFILMSHLLVSFELKHQPLCQVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CTQ0</accession>
    <name>A0AAD5CTQ0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_032971</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006993">
        <property type="protein sequence ID" value="KAI7746361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CTQ0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006754">
        <property type="term" value="P:ATP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34264">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT B, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34264:SF3">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT B, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7746361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="62" mass="7222" checksum="D8E4B76C091FB904" modified="2024-05-29" version="1">MLEKGHEWPEKARACLGKGEIEANELLTSGYSEIKRQKMYLIDSTYKTLKQLENYKNKTINS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CU10</accession>
    <name>A0AAD5CU10_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CYTH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_027886</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006619">
        <property type="protein sequence ID" value="KAI7747962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CU10"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016462">
        <property type="term" value="F:pyrophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02028">
        <property type="entry name" value="UMPK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033469">
        <property type="entry name" value="CYTH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023577">
        <property type="entry name" value="CYTH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006083">
        <property type="entry name" value="PRK/URK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10285">
        <property type="entry name" value="URIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01928">
        <property type="entry name" value="CYTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00485">
        <property type="entry name" value="PRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00988">
        <property type="entry name" value="URIDINKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55154">
        <property type="entry name" value="CYTH-like phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51707">
        <property type="entry name" value="CYTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="331"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="600"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="domain" description="CYTH" evidence="3">
        <location>
            <begin position="249"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="477"/>
            <end position="504"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7747962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="644" mass="72666" checksum="E7916BA47A50F756" modified="2024-05-29" version="1">MMGQDNSNVESNSRKQRLLKDQVRLVKRKDNDRFEIVQIKDSLSFEKGFFVVIRACQMLAQKNDGIILIGLAGPSGAGKSAFTDKILSFMPSVAVISMDNYNDATRVIDGNFDDPRLTDYDTLLKNILDLKEGKTIEVPIYDFKSSCRTGYRTLEVPSSRIVIIEGIYALSEKLRPLLDLRVSVTGGVHFDLVKRVLRDIQRAGQEPEEIIYQISETVYPMYKAFIEPDLQTAHIKIVNKFNPFTGFQNPTYILKSSRNVSVEQVKAAMSEEGTESLEQIYDIFLLPPGEDPETCQSYLRMRNREGKYNLMFEEWVTDPPFIISPRISFEVSVRLLGGLMALGYTIASILKRSSHVFKYESVCVKIDWLEQVNRRYVQVLGRDRLGVRCVAERLGLEGSYIPRTYLEQMKLEKLLNEVMEMISSPRGSITLPTRVNKFRKSGLSHSYSTASKDLISIKRFEDTSQEPTRALASQGAISQLSEQISTLNDRMDEFTSRIDELNSQFTRGMNYGSQSQQKIDACNGSASASTSYFTGSAVRHSSSSTQLVKDSNLVEEITGIARNQLKIMHQLDIMNDNLRGKMDERSRQEREVKKSQRSKLDGVVVSLLLAFACGGVGVFLFKGESGPDFLDLVLLQSRMVILGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CXM1</accession>
    <name>A0AAD5CXM1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">FAD-binding PCMH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_013919</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oxygen-dependent FAD-linked oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006531">
        <property type="protein sequence ID" value="KAI7748401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CXM1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.43.10:FF:000004">
        <property type="entry name" value="Berberine bridge enzyme-like 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.462.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.10">
        <property type="entry name" value="Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012951">
        <property type="entry name" value="BBE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016167">
        <property type="entry name" value="FAD-bd_PCMH_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32448">
        <property type="entry name" value="OS08G0158400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08031">
        <property type="entry name" value="BBE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042156118" description="FAD-binding PCMH-type domain-containing protein" evidence="8">
        <location>
            <begin position="25"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="9">
        <location>
            <begin position="79"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7748401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="545" mass="60740" checksum="BEEDDB12CC85A610" modified="2024-05-29" version="1">MSTITFISLTYFLCSCFILSKGASDPNTIYKSFLQCLPAQSPQPDPSFSAIVYGSIANSSSYTTVLQEYIKNRRFNTTTTPKPAIIITPTKEFHVQAAILCARKLGIQIKIRSGGHDYDGISYVSSEKNFIVLDMFSFRNVDVNIATETAVVGVGAQLGELYYRIWEKSKVHGFPAGVCPTVGVGGHLSGGGYGTMLRKYGLSVDHIIDAKIVDVKGRVLDRKSMGEDLFWAIRGGGGASFGVILSYTVKLVPVPAVNTVFRIMKTVAENATDLVHKWQTIVPTMNNNLFIRILLQPVTVNKQKTLRASFIAHFLGDSATLVAEMTKIFPELGLKKENCTEVSWIQSVLYWANFDIKTTPVKILLNRHSDKVNFLKRKSDYVQTPISKSGLTSIFNKMVELGKVGLVFNSYGGRMSEVASNATPFPHRAGNLFKIQYSVNWNEGDPELEKNYMNQSRVLYKFMTNYVSKNPRGAFLNYRDLDIGVMTGTGNNGYNSGKVYGVKYFMGNFDRLVKIKSVVDPDNFFRNEQSIPVISHKSRKMTMLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CY90</accession>
    <name>A0AAD5CY90_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Leucine-rich repeat-containing N-terminal plant-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_028939</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006150">
        <property type="protein sequence ID" value="KAI7750468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CY90"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051707">
        <property type="term" value="P:response to other organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000383">
        <property type="entry name" value="Leucine-rich repeat receptor protein kinase EMS1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000095">
        <property type="entry name" value="LRR receptor-like serine/threonine-protein kinase GSO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013210">
        <property type="entry name" value="LRR_N_plant-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052595">
        <property type="entry name" value="LRRC69/RLP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48057">
        <property type="entry name" value="LEUCINE-RICH REPEAT SERINE/THREONINE-PROTEIN KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48057:SF19">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING N-TERMINAL PLANT-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08263">
        <property type="entry name" value="LRRNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Leucine-rich repeat-containing N-terminal plant-type" evidence="8">
        <location>
            <begin position="2"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="9">
        <location>
            <begin position="400"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="864"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7750468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="864" mass="96381" checksum="F8B03F7F60F34589" modified="2024-05-29" version="1" fragment="single">RLALLKFKRSIQDDHGMLSSWGVGSDCCSWNGVRCDGITGSVVSLLLRGNYYPVDDYDADYQRGNYIPNGGDDLEEEYYLVGDKVNAYLAELRHLQHLDLSGNYFTGSQIPKFIGSFQQLRYLNLSNAMFSGIIPHHIGNLSNLKVLDLGSASYSLYGLTSNDIAWVSGLSSLEHLDLTIVDLSQANHVDMVFYMIPSLTYLKLSRCELSNVNLGLHLNSSKIPPNIKHLDLSFNSFEGQLPRFFKNMTSLTFLDLSDYNLSLAQDSVNQLNMIPSLSELHLSFCQIHNAHFSPTYFNFSAHSHIQYLDLSLNSIEGRIPSALMNITSLKVLDLLLNSVNSSIPVMPNLLKLDVTNNNFEHIELVGIWRQCHLKELRLSANYFGGEMMDPSSNTSECSHYALEVLESDHNELNGSIPESIGKLNNLRVLSLQSNQFTGSIPKALERLRSLKVLDLSNNGLTGSVPSFLGKLTELDLSQNKLSGSIPESLGRLTGLTHLRLQSNLLTGPIPVSVGQLNKLTKLDVSNNSLEGVVNKAHFANLSMLKLLDTSANHKLIFNISHEWVPPFQLKLLQLSSCKIVNGFPQWLQNQRKLEGLVLSNASLYGPLPTWLRNMPMLYALDLSHNKLAGSLINLPFNENTRLLLLQDNLLNGSIPRLVCRTTSLQILDVSNNQISGNIPDCLENLQNIEMLILSSNRLSGVLPSSFGNINSILRWLKLDDNNFSGEFPRDLGQLRNLEVLDLGNNKFSGIIPKWIGKRHSALKILRLHKNNFTGGIPHSLCNCSYLHILDIAHNNLIGSIPHCFGELSWMVEATGGGFWGIIGVLAFKKQWRQKLFMIAEVIMDKVFVAVAVRISKIKRAREAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CZB5</accession>
    <name>A0AAD5CZB5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_008711</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005967">
        <property type="protein sequence ID" value="KAI7751133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CZB5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="58"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="149"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="162"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7751133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="189" mass="21204" checksum="39648D0861E01BE5" modified="2024-05-29" version="1" fragment="single">GVSFVDVTSINDDLEMFEDHSEEKIDVSSPNLVSDKQEFKEVDETDANLEVAVAEVSSSLEEVKREQDEKEVKVPVSSSDLEGKSTTEAQDSQLILPEEVIISRCCNDSKLLEVPSVQPSKPMMGAKPKRRLRPASSVTLKNINILEFDDATTMPKQHRHGKRDENEDGRKRTQGSNALLRILQQNLRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D067</accession>
    <name>A0AAD5D067_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Wall-associated receptor kinase galacturonan-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_020514</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005982">
        <property type="protein sequence ID" value="KAI7751109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D067"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030247">
        <property type="term" value="F:polysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025287">
        <property type="entry name" value="WAK_GUB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33491">
        <property type="entry name" value="OSJNBA0016N04.9 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13947">
        <property type="entry name" value="GUB_WAK_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Wall-associated receptor kinase galacturonan-binding" evidence="3">
        <location>
            <begin position="2"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7751109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="302" mass="32992" checksum="8D01476EE3EA70EA" modified="2024-05-29" version="1" fragment="single">MCGNVRIPYPFGISANCSVNQWYIVDCKSSTPYLRALNHPQVLGMSLENETITIRTPKISNCQKIVGNSTETVSFDLGRSPFFFSEDHNKFVFEGCGTATLMVNGSVVTACSTACRGVILRDGNNCFGIGCCQAKISNLLNSYSIHVTGLEEEDGGCGSAFLVDEMSYDEGRFNVKNSSFIPVTLFWTLTDSDQVTCCGDQPPEENTIDMFNGTSTTVDTRRCYMLSSITKGNPYLLDGCVESEHDEEDPSGECKSCKDSGGYCGSDAIYNLDGSVFDHTITCYHYNRPSLGVILGICLFLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D095</accession>
    <name>A0AAD5D095_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_023492</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005860">
        <property type="protein sequence ID" value="KAI7751658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D095"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="75"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="171"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7751658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="189" mass="21187" checksum="FE7E7FCDE0534271" modified="2024-05-29" version="1" fragment="single">VKFSGLEEHEGETSHVQDRKLTSQRTRSFNGDGRRVQNWYRGQSSRELSYDYDSDYMERDEFRTAVAAAAFAVNSVEERRRATRRRDSSLNKPKSKQEDGAVSLTKRIRERIPSSSSSAAAARNKTKNTEDHNVPISTPSFLSRKFSELQDKLPQRARSPANTEPKPGASFASSSGDQKPTNQPATPPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D0T7</accession>
    <name>A0AAD5D0T7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Late embryogenesis abundant protein LEA-2 subgroup domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_009207</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005766">
        <property type="protein sequence ID" value="KAI7752026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D0T7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055276">
        <property type="entry name" value="NHL41-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48436">
        <property type="entry name" value="2, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48436:SF1">
        <property type="entry name" value="2, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="124"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="69"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7752026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="310" mass="34393" checksum="B78D503F3B224E8E" modified="2024-05-29" version="1">MLHTKSESDITSLDPSSPSRSQKGLVYYVQSPSRDSQDGDKSCSIQATPNFRSPMESPSHPSVGRHSRNSSSSRFSGIFRSHSGRKVHRKRNDKGWPECNVIVEEGKYEGYDDEKKVTRRLQALMALLSFIVLFTILCLIIWGAARPYKAEITVKSLAVNSLYIGQGSDSSGVITKLLTINGSLHLGVHNPATFFGIHVSSTPVNLIFSEIVIATGQLKKYYQQRKSRRTAIVNLEAIKVPLYGAGSSLVVSDTGVFEIPLKLEFVIRSKGEVVGKLVTTKHRRQISCNMTLPSNTTKPIRFRKSSCIYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D4F9</accession>
    <name>A0AAD5D4F9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_023414</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005538">
        <property type="protein sequence ID" value="KAI7753137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D4F9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="9"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7753137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="29" mass="3237" checksum="37364B312239F394" modified="2024-05-29" version="1">MQLTDICLPSNGHIQSSKQNDKEHCAIQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D5L2</accession>
    <name>A0AAD5D5L2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_025585</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010002671">
        <property type="protein sequence ID" value="KAI7754703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D5L2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46151">
        <property type="entry name" value="NEP1-INTERACTING PROTEIN-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46151:SF28">
        <property type="entry name" value="ZINC FINGER, RING_FYVE_PHD-TYPE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="7">
        <location>
            <begin position="116"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7754703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="161" mass="17611" checksum="32DCC1AC019E6541" modified="2024-05-29" version="1">MTTAQMVKGAIEVVHGSRPVLQPASNITTSDSSTDADLGLQLTRSASPNQQLLPLGRTTLPIHIVADDNFEPKHLPDHDNMFLITKVGSGEKLIKLPKNKISNHNILDTSGNETCCSVCLQDFEVGNSAGTFPKCEHKFHPECIYQWLLTNNSCPVCRTTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D622</accession>
    <name>A0AAD5D622_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_032272</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005404">
        <property type="protein sequence ID" value="KAI7753529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D622"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13999">
        <property type="entry name" value="STKc_MAP3K-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000180">
        <property type="entry name" value="serine/threonine-protein kinase STY46-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF182">
        <property type="entry name" value="DUAL-SPECIFICITY KINASE TKL-PL-5 FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000654">
        <property type="entry name" value="Integrin-linked_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="59"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="151"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7753529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="389" mass="43785" checksum="C8C1797A5E777394" modified="2024-05-29" version="1">MSGSEGSSGHSADAPAKRKDKAKVSRTSLILWHTHQNDAAAVKKLLEEDRSLVNARDYDNRTPLHVASLHGWIDVAKCLIDYGADVNAQDRWKNTPLADAEGAKKRSMVELLKMYDGVSFGQDGSHSEPKPVLPPLSNKCDWEIDPMELDFSNSHIVGKGSFGEIVRASWRGTPVAVKRILPSLSDDRFVIQDFRHEVNLLVKLRHPNIVQFLGAVTEKKPLMLITEFLRGGDLHQCLKEKGALNPTTAINFALDIARGMAYMHTEPNVIIHRDLKPRNVLLVNSNADHLKVGDFGLSKLIRVQNSHDVYKMTGETGSYRYMAPEVFKHRKYDKKVDVFSFAMILYQMLEGDPPLSNYEPYEAAKCAAEGLRPIFKSKNYTLELREYVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D9Y2</accession>
    <name>A0AAD5D9Y2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_020700</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010002354">
        <property type="protein sequence ID" value="KAI7754866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D9Y2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="261"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="261"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7754866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="295" mass="32915" checksum="879A8131F78D740A" modified="2024-05-29" version="1" fragment="single">MYSRIDRDGGSQQGPKGTIKGFETKLGVWLAADLSGKVDFTFRQFIPKSNNKFLFCIINISPPDSLSASVSGLLLRRWVTNRNADPLHTTFCLFFFCPFPSDTDYDHMLTPKLEGIRISYMIFDVLLRWRTAWGWGHHPFQSAFNDIRISLRGRRRNRGQRSCQSTMSVTRLTKSCERQVVAATRDIASKKESPNTNTAAGSWTGPVVCPSVHRKQGGIHGVPAVNGSFTKDKTLRSGMWGFRGVYSHRVRIHGRPKSKTVTCTFSSSSNGNGSMAESFNENDSDYIDSSVVEAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DA30</accession>
    <name>A0AAD5DA30_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_006043</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010003865">
        <property type="protein sequence ID" value="KAI7754355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DA30"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008725">
        <property type="term" value="F:DNA-3-methyladenine glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.30">
        <property type="entry name" value="Hypothetical protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005019">
        <property type="entry name" value="Adenine_glyco"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31116:SF29">
        <property type="entry name" value="DNA GLYCOSYLASE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31116">
        <property type="entry name" value="OS04G0501200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03352">
        <property type="entry name" value="Adenine_glyco"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="DNA-glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="83"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR605019-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7754355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="186" mass="20835" checksum="4ED53FBB317B4A2F" modified="2024-05-29" version="1" fragment="single">MSKENMRRNIVEKKNNSTREKEKLSSTQNLLSKHLKKVYPVGIHKTSSLLSLSSLSLTLSHNSSGSFTDSSSTLEQTISSALQLIAPSPTRSPSPSPTPARRDLPAAKTSTHGLVPQPSLDPSIGEDGLRRCNWITKNSDKLYVQFHDECWGVPVYDDNQLFELLSLCGMLMDYNWTEILKRKNLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-04-02" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G1D6</accession>
    <name>A0AAD5G1D6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein high chlorophyll fluorescent 107</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_021733</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012153">
        <property type="protein sequence ID" value="KAI7724810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003727">
        <property type="term" value="F:single-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044624">
        <property type="entry name" value="Mbb1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44917">
        <property type="entry name" value="PROTEIN HIGH CHLOROPHYLL FLUORESCENT 107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44917:SF1">
        <property type="entry name" value="PROTEIN HIGH CHLOROPHYLL FLUORESCENT 107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="140"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="522"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="98"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7724810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="589" mass="65017" checksum="94DC53B80774F47B" modified="2024-05-29" version="1">MKKLLSSPSQQHFPLFSPSNGTSNLHFPTFIKPSFSHSPFQSLSALDSSSSVPAPVLLQTDAAEFNAGRFSSSTGALTNSSSVRFAKSSDTAKRTLEEPPSSAASVPAPVVPETVAAELNSNSSSVERTLEELLVVRRPVKEATNDGEDEGDDDVEMEEGFGAASSSSSSVVDAELSRFAKKMPIFEPMRIDASSGEKRLGVNLDIALYRAKVLGRSFRYKEAEEVLQKCISYWPEDGRSYVALGKILGKQSKADEARAVYEKGCQATQGENAYIWQCWAVLENKMGNIRRARQLFDAATVADKKHIAAWHGWAVLELKVGNLKQTDMSKHGTCLGRLLNVIPKVVPVGLLGLSLRYNKKIIARLDSFLRKETXXXXGHALNPRDPVLLQSLGLLEYKHSAPNFARALFRRAAELDPRHQPVWIAWGVLEQRVGNLSAARRLFRSSLNINSQSYVTWMTWASLEEDEGNAIRAEEIRNLYFQQRTEVVDDGSWVTGVLDIIDPAIDSIKKLLNINQNQYNKTRDSLTSEDGSDPVGEESGTGFDLDGFISEKLSLDASKLDIDFESKSKNLVRASPRKIWRASESTVSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G576</accession>
    <name>A0AAD5G576_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">HTH myb-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_001310</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MYB-CC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011023">
        <property type="protein sequence ID" value="KAI7729280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G576"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000002">
        <property type="entry name" value="Myb family transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025756">
        <property type="entry name" value="Myb_CC_LHEQLE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006447">
        <property type="entry name" value="Myb_dom_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046955">
        <property type="entry name" value="PHR1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01557">
        <property type="entry name" value="myb_SHAQKYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31499:SF52">
        <property type="entry name" value="HOMEODOMAIN-LIKE, MYB-CC TYPE TRANSCRIPTION FACTOR, LHEQLE-CONTAINING DOMAIN PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31499">
        <property type="entry name" value="MYB FAMILY TRANSCRIPTION FACTOR PHL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14379">
        <property type="entry name" value="Myb_CC_LHEQLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="7">
        <location>
            <begin position="32"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7729280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="266" mass="29634" checksum="2A4E96B9D8F988FB" modified="2024-05-29" version="1">MYTNIHSIPLEDFPRPLTATNLVGDPCLVLTTDPKPRLRWTAELHERFVDAVTQLGGPDKATPKMIMRTMGVKGLTLYHLKSHLQKYRMGKQSCKEFSENSKEVSCIAESQDMGSSTSSSARMMPQDLNDGFEVTEALRVQMEVQRRLHEQLEVQQRLQLRIEAQGKYLQSILEKACKALNDQAIATAGLEAAREELSDLAIKVNNDCLPPLNQTPSLTQVAAHEHGPTRLGDCSVDSCLSSQAAVLNKRQRAMFMQAEWKAANIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G5Z4</accession>
    <name>A0AAD5G5Z4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Exostosin GT47 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_028700</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 47 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011152">
        <property type="protein sequence ID" value="KAI7728771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G5Z4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004263">
        <property type="entry name" value="Exostosin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040911">
        <property type="entry name" value="Exostosin_GT47"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11062">
        <property type="entry name" value="EXOSTOSIN HEPARAN SULFATE GLYCOSYLTRANSFERASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11062:SF249">
        <property type="entry name" value="OS08G0438600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03016">
        <property type="entry name" value="Exostosin_GT47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <feature type="domain" description="Exostosin GT47" evidence="6">
        <location>
            <begin position="50"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7728771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="248" mass="27672" checksum="14723BBDE1347698" modified="2024-05-29" version="1" fragment="single">PRSIYSLIVVDRGSVEPELDSCKGLCDGSKAVKVTAGCDPNQAVLKGGVIRQELYYLLKDEKDVHFTFGSAGGGGIRKASTGMAASKFCLNIAGDTPSSNRLFDAIVSHCVPVIISDDIELPYEDILDYSKFSIFVPASDACKNGYLINLLRGVKREKWIEMWENLKQIAPHFEYQLAALSLAVESLVLAFECAFHMYKKWKQRLQHINSYLTCVRHCQPKELRCRLYIVSTLRLDSEIESRSAGAKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G5Z8</accession>
    <name>A0AAD5G5Z8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_001423</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the iron/ascorbate-dependent oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010835">
        <property type="protein sequence ID" value="KAI7730135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G5Z8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050295">
        <property type="entry name" value="Plant_2OG-oxidoreductases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47991">
        <property type="entry name" value="OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0408">Iron</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="5">
        <location>
            <begin position="190"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7730135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="362" mass="41242" checksum="F7EEC28823C35ABE" modified="2024-05-29" version="1">MYIEMKEDKDARWFELECVPKEYIFSEEYRPQNIDSPVCDSIPVISLAKSEAANEHSQPVEEILKASQEFGFFQVIDHGIPEKMMIDAMNVLKEFFDMQAKYASNGTVTPKKGYIYTTSTDFTKDGVYLWRENLKHPCHPLEECVPLWPDTPPRYQEVIAAYLVEIQKLSSRILEMICEGLGLEKGYFKDISEVQLLASNFYPPCPDPSLTLGILPHIDPSFITILYQGEATGLQFMKDDQWIKIGAIPNAFVVNIGNQLEVCILSIIMVNGSSSYLWMQIISNNKFKSAKHRVVTSKHETRRSIATFVNPSPNCIIEPAKILVNESEPALYKPTQYKDFVQYNNSFGDYTAAIQKALDEES</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-02-05" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G6B1</accession>
    <name>A0AAD5G6B1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">THO complex subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_000294</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the THOC2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010826">
        <property type="protein sequence ID" value="KAI7730194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000445">
        <property type="term" value="C:THO complex part of transcription export complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040007">
        <property type="entry name" value="Tho2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021418">
        <property type="entry name" value="THO_THOC2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021726">
        <property type="entry name" value="THO_THOC2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032302">
        <property type="entry name" value="THOC2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21597:SF0">
        <property type="entry name" value="THO COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21597">
        <property type="entry name" value="THO2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11262">
        <property type="entry name" value="Tho2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11732">
        <property type="entry name" value="Thoc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16134">
        <property type="entry name" value="THOC2_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="THO complex subunit 2 N-terminal" evidence="9">
        <location>
            <begin position="38"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="domain" description="THO complex subunit 2 N-terminal" evidence="9">
        <location>
            <begin position="442"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="domain" description="THO complex subunitTHOC2 N-terminal" evidence="8">
        <location>
            <begin position="585"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="domain" description="THO complex subunitTHOC2 C-terminal" evidence="7">
        <location>
            <begin position="907"/>
            <end position="1202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1244"/>
            <end position="1728"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="930"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1250"/>
            <end position="1267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1268"/>
            <end position="1278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1300"/>
            <end position="1314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1333"/>
            <end position="1344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1374"/>
            <end position="1389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1409"/>
            <end position="1537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="1541"/>
            <end position="1551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1559"/>
            <end position="1569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1580"/>
            <end position="1592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1599"/>
            <end position="1643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1687"/>
            <end position="1696"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1706"/>
            <end position="1728"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF11262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF11732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7730194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="1728" mass="197619" checksum="D8ACB162A4B6F3D9" modified="2024-05-29" version="1">MSLPSLECKYITDECLREWKNNGSTFKPPSSAPVLRYLYELCWNIVRGELPIQKCKLGLELVEYSDKVSEDEVSSHLADIVSQMAQDITMPGEYRARLIKLAKWMVEHSVVPLRLFHERCEEEFLWDSEMIKIKAADLKSKEVRVNTRLLYQQTKFNLLREESEGYAKLVTLLCQGSEVCSPNASAATVGIIKSLIGHFNLDPNRVFDIVLECFELQPENNVFLNLIPIFPKSNASQILGFKFQYYQRMEVNNTVPFGLYQLTALLVKKEFLDLDSIYAHLLPDDDEAFDHYNVFSAKRLDEANKIGKINLAATGKDLMDDEKQGDVTVDLFAALDMESEAVTERSSELENSQTLGLLIGFLFVDDWYHANILFRRLSPLNPVEHIRICDGLFRLIEKTISPAYELVRQPQLQTTEGGSDLMEADTNITKKSFINLPKELFEMLTSVGPYLHRDTLLLQKVCRVLRGYYLSALKGVSSGDDFNLSENRTPRLHLKEARLNLEEALGACLLPSLQLIPANPAVGQEIWAVLSLLPYEXXYRLYGEWEKDDECNPMVLSAKQTAKLDTRRILKRLAKENLKQLGRMVAKLAHANPMTVLRTIVHQIEAYRDMIAPVVDAFKYLTQLEYDILEYVVIERLVQGGREKLKDDGLNLSDWLQSLASFWGHLCKKYPSMELRGLFQYLVNQLKKGSGIELVLLQELIQQMANVQYTENMTEEQLDAMAGSETLRYQATSFGATRNNKALIKSTNRLRDSLLPKDEPKLAIPLLLLIAQHRSVVVIKADSPHIKMVCEQLDRCHGSLLQYVEFLCGAMTSINSYAQLVPDLHDLIHLYHLEPEVAFLIYRPVMRLFRCQTTSDVLWPLDCDESSSSSAPGKEGELILDLGLPRKPIMWSDLLDTVRTVLPSKAWNSLSPDLYATFWGLTLYDLYVPRNRYESEIAKQHAALKALEELSDNSNSAITKRKKDKEKIQESLDKLTSELQKHEEHVASVRRRLAREKDKWLSSCPDTLKINMEFLQRCIFPRCTFSMPDAVYCAMFVNTLHSLGTPFFNTVNHIDVLICKTLQPMICCCTEYEVGRLGRFLFETLKTAYYWKSDESIYERECGNMPGFAVYYRYPNSQRVTYGQFIKVHWKWSQRITRLLIQCLESTEYMEIRNALILLTKVSNVFPVTRKSGINLEKRVAKLKSDEREDLKVLATGVAAALAARKPSWVTEEEFGMGYIDFKPAPVAAKSAYVTPQTTNIPLNEIANGKTGTAQHHDSGASNTSRTKPTENKHEKGKGGSLSNGSDMLAGPSRSAEGQKQIDDSGNRVLDENPAKGPAVKRSVPAASVQKQAKQDGVKTETKPKPGGSVHEPSAQAKGSSRSSDPETGVSKLAESRKDDSSEPMDGQRRTSSRPTHSPRTENSYALKTNDKPTKRMSPAEEQDRFNKRRKGEHDLRDIEPEVRFSDRERLGDPRASEDEPIRNSDKQLEKPKVERYDREYRERYHGDDVIEKSRDRSMERYSRERSVDRDSDKGSKDDRNKGRYNDNLADKDDRFRGQSLPPPPPLPPHVVPQSLNANRRDEDSDRRFGTARHSQRLSPRHDDRERRRSEENMLLSQEDAKRRREEEIRERKREEREVFSNKMDDRERDMEREKINATKEELDVNASKRRKLKREQHMASEPGEYSPAASAPAPPLHINMTSSYDVTRDRGDRKAPVPRNVYMEEPARMHAKDVPTKMTRRDSDPYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GA92</accession>
    <name>A0AAD5GA92_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BSD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_004076</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009730">
        <property type="protein sequence ID" value="KAI7734427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GA92"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000439">
        <property type="term" value="C:transcription factor TFIIH core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005607">
        <property type="entry name" value="BSD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035925">
        <property type="entry name" value="BSD_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027079">
        <property type="entry name" value="Tfb1/GTF2H1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12856">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00751">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140383">
        <property type="entry name" value="BSD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50858">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BSD" evidence="2">
        <location>
            <begin position="76"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7734427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="516" mass="58078" checksum="D7C031C1AC54EDB3" modified="2024-05-29" version="1">MTDAQVVKRAKYKSSVKDPGLRGHKSSKEGANRPALLNLTQDQGNYIFEFENFSDREICRDFVAKAIAFSGEVGGPTSDKAVPQKDEQLSSAEMERRIKLLQEDIELQKLHKQFVIGDVLSETEFWATRKKLLDVNASRKAKQRVGLKSEMIFNVKPSSDGQSNRVTFNLTPEMIHQIFAEKPAVRQAYINFVPNKAAYVSQAAEDEELAVFLRQDAILANETRQKIRKVDPTLDMEADEGDDYTHIPGHGLANEDIKDELEAQYEPFKRSFLQDINRHAAVVLEGRTIDVETGDTRSVAEALASTKRVELAKEASDGNVLQDRIDRIVRMAEIEDLQAPRDPPVAPLCIKDPRDYFDSQQVNALNQLSGTRQMKSRLSTSEAYGSLRAHISEIRTTGFSDPIVRPEVAFQVFNSLTQNISSSKYQLGKNPHESILDTLPSVTKEELLLVSRLRDAMSKIYPKLQEIKESVQSDTRHQVSLLVQPMLQALDAAFAHYELDQQKRYAKGGEKPNGYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GAV2</accession>
    <name>A0AAD5GAV2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Peroxisomal membrane protein PMP22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_032910</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the peroxisomal membrane protein PXMP2/4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009542">
        <property type="protein sequence ID" value="KAI7735177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GAV2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007248">
        <property type="entry name" value="Mpv17_PMP22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 2, PXMP2 MPV17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266:SF86">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PMP22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="38"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="90"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7735177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="179" mass="20092" checksum="F801FE3A221107B1" modified="2024-05-29" version="1" fragment="single">LQQHPLRTKALTAGVLAAISDVVSQKLSGIQKLQVRRLLLKVLFGCAYLGPFGHFYHTLLDKLFKGKKDNTTVVKKVVLDQLTASPWNNLMFMLYYGFVIEGRITVIVKTMDPRKIQNQEGIPFSSVCIMDVLACNRVDKPPPRATSIPCHCPKLCRNVLGDILESKSKINDIDQRLSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GB33</accession>
    <name>A0AAD5GB33_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_020470</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CLPTM1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009612">
        <property type="protein sequence ID" value="KAI7734934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GB33"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008429">
        <property type="entry name" value="CLPTM1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21347">
        <property type="entry name" value="CLEFT LIP AND PALATE ASSOCIATED TRANSMEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21347:SF0">
        <property type="entry name" value="LIPID SCRAMBLASE CLPTM1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05602">
        <property type="entry name" value="CLPTM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="179"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="189"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7734934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="484" mass="54987" checksum="910A0EF9025DD98D" modified="2024-05-29" version="1">MTPPAAVEGGGGDGERQQQQQSIGQMLQGIIRIALIFYFASKFFSSSSSNNNNNKPSSSSSSSSVQISNLFHKSQPLDMWFYLSEHDKFNDFGNEDLLIWHETNIPYAVWGPESTRTLSMKYYPSQALKHNGSLFAHVFFARSGYPADPNDPEYQPLAAFGLKHPVVTYLPKSKANKKKSLLGNSKDTNEDDSVSEVVEEPQADLKDDGPAEYISYWKPNITINLVDDFTKYSQRSVPPNVAPYMNIEPTTGNYYPTVYFNEFWLLRDKLTALNDTVTELPLHLEVAPISMTKWQLFLQIDQSFQIHRSYGSMLEGEADELKRVFVEGNPYLLGITMLVSMLHSVFDFLAFKNDIQFWNKNKSMEGLSAKSVVVSFICQLIIFLYLLDNDTSWMILASSGIGVCIEFWKIGKAMHIEVDRSGSIPKLKFSDRDTYAGNKTKEYDDLAMKFHYDVPTAVHQLQAKISRSFTMEADDIQVSQYNYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GCG0</accession>
    <name>A0AAD5GCG0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_009011</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009096">
        <property type="protein sequence ID" value="KAI7737172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GCG0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7737172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="131" mass="15004" checksum="E2DEB6C59D276716" modified="2024-05-29" version="1">MVVWQREGGVPVQTPGFSETGERNLEGSNDVDSESGVKKSQWSYSDSLKAGENVLLCFTADDKMELEVIQQWKNDCHSHLFKLDVFPTLKDHKRDEAGCEHRWWHIADAVGSQHPVQQVLLSIYQLLYEPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-10-08" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GCK3</accession>
    <name>A0AAD5GCK3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N-end aminoacyl transferase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_028813</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009060">
        <property type="protein sequence ID" value="KAI7737350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004057">
        <property type="term" value="F:arginyl-tRNA--protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030700">
        <property type="entry name" value="N-end_Aminoacyl_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007471">
        <property type="entry name" value="N-end_Aminoacyl_Trfase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21367">
        <property type="entry name" value="ARGININE-TRNA-PROTEIN TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21367:SF1">
        <property type="entry name" value="ARGINYL-TRNA--PROTEIN TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04376">
        <property type="entry name" value="ATE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-end aminoacyl transferase N-terminal" evidence="1">
        <location>
            <begin position="33"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7737350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="183" mass="21286" checksum="9AF7943CE6832B1C" modified="2024-05-29" version="1">MADKWNIKTEASNSSTNTIDKSIVEDYGRQRRSCGYCKSRSNTSIADDFEAFNSDSRIALNLDSEFDNPVRESVIFGSKFYTIGYAQFYELDRESYAXXXXRYAGLWAYSMTVHDYQALLDRGWRRSGCYLYKPVMEKMCCPSYTIRLKANDFVPSKEQVRVAKRMQRFLHGSLNIKKSDEQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GD07</accession>
    <name>A0AAD5GD07_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_005205</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009457">
        <property type="protein sequence ID" value="KAI7735658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GD07"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010017">
        <property type="term" value="P:red or far-red light signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044273">
        <property type="entry name" value="PIF3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46807:SF8">
        <property type="entry name" value="TRANSCRIPTION FACTOR PIF1-LIKE ISOFORM X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46807">
        <property type="entry name" value="TRANSCRIPTION FACTOR PIF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="6">
        <location>
            <begin position="416"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="204"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="278"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="400"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="70"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="245"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7735658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="579" mass="63925" checksum="5609455D00AF4448" modified="2024-05-29" version="1" fragment="single">ILINSGFIRVFVSNLILHIMNHYVPDFATDEDYLLQDSSSLKRHKKSTMGDDDIMELLWQNGQVVMHNQNQNQRSVANNKKPETTTSGKEIRSGVMEDETTPCNLFMQEDEMASWLHYPIDDDNNNNNNNLDMYLHNNNNNNNIMYQGLPSSNSVIIPSSSSQPPLPPVVSVSVPPPRPPIPPVKRVEEEPSQPKFANFLHFSRPSRKLTGTGTGTGSGVGTSNKALPAMTQSTVVESNDRPVNEAQQSRASRVTDSNALVSGANVGFRGSIMSSFGGVGASSGGREPETYDLSASSSPGTGGSGASASVEPLSQKQPQPETAADRKRKGVDTDDTECYSESSIAKFLLAVQTKFIDCRLAAEFINEPMKYKFQCTFGVLLKLYLPRRTTASHDAEFEYHDAKKQKRGSASAKRTRAAEVHNLSERRRRDRINEKMKALQELIPRCNKMMSMGYNMVPMMFPGVQRYMPAMAPMGMGMGMEHVGMNRPMVPYPQVLPGSAMPNPAAAAAHLGQRYPVPRFQMAQVPIMGPTSSPTANMSDPMMNSYPMQNANQPRVPFADPYQQYIGLPQTQMLHPQAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GE71</accession>
    <name>A0AAD5GE71_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_007557</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008860">
        <property type="protein sequence ID" value="KAI7738174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GE71"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010011">
        <property type="term" value="F:auxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009734">
        <property type="term" value="P:auxin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010152">
        <property type="term" value="P:pollen maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22159">
        <property type="entry name" value="F-box_AtTIR1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.50:FF:000006">
        <property type="entry name" value="Transport inhibitor response 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000029">
        <property type="entry name" value="Transport inhibitor response 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041567">
        <property type="entry name" value="COI1_F-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041101">
        <property type="entry name" value="Transp_inhibit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16134">
        <property type="entry name" value="F-BOX/TPR REPEAT PROTEIN POF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16134:SF92">
        <property type="entry name" value="LEUCINE-RICH REPEAT DOMAIN SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18511">
        <property type="entry name" value="F-box_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18791">
        <property type="entry name" value="Transp_inhibit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0927">Auxin signaling pathway</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="COI1 F-box" evidence="7">
        <location>
            <begin position="21"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="Transport inhibitor response 1" evidence="8">
        <location>
            <begin position="80"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF18511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF18791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7738174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="583" mass="65072" checksum="3654D73D21897318" modified="2024-05-29" version="1">MDPAAAVADVTGTDADLTKTTTFPDEFLKQTFSLINSDKDRNSVSLVCKDWYNVERMNRRHVSIRNCYSVSPETVTARFPAIRSVTLKGKPRFSDFNLVPDDWGADVQSWLNVFATAYPLLEELRLKRMMVSDESLEFLALNFDEFKGLSMLSCEGFSTLGLEAIATHCKNLTELDIQENGIDDLGGDWLSYFPENLTSLEVLNFSTLTSEVSFDALESLVSRCKSLRVLKVNRKVNLEQLQKLLVLAPQLTELGTGTFMEELVPSSVNGLESAFSKCGNLVALSGFWDATLLHLPVVYPVCENLTFLNLSYANIEADELEQILTRSKRLVRLWVVDTVGDKGLEAVGSHCPLLEELRVFPVDPFDQQLGVTESGLVAVSNGCPKLHYILYFCQRMTNAAVITVAQNCPNFTHFRLCIMNPREPDYLTNEPMDEAFGAIVKTCTKLQRLSVSGLLTDLAFEYIGKYAKNLETLSVAFAGDSDLGMEYVMRGCPKLKRVEIRDCPFGDSALLSGLTQYESMRSLWMSDCNVTMDGCKKLAEEMSRLNVEVIKEGDGDDRLANKVYVYRTVAGPRKDAPPFVVTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GEA7</accession>
    <name>A0AAD5GEA7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_022994</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009073">
        <property type="protein sequence ID" value="KAI7737261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GEA7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14703">
        <property type="entry name" value="bZIP_plant_RF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000009">
        <property type="entry name" value="probable transcription factor PosF21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044759">
        <property type="entry name" value="bZIP_RF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13690:SF157">
        <property type="entry name" value="BASIC-LEUCINE ZIPPER DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13690">
        <property type="entry name" value="TRANSCRIPTION FACTOR POSF21-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="8">
        <location>
            <begin position="287"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="305"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="13"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7737261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="416" mass="45520" checksum="FF37DE1461C8559B" modified="2024-05-29" version="1">MPPTHPQIPPLSPCSQIPLTHSHGNNQQTEGSHDLTLGVGPSHSQPSFYSVDSLPPLSPCSYRSIGVVGDVSLPPRKTHRRANSDIPFGFSTILQSSPPLLSLRGTKGVYVKHEANWEKSGGEISNGEGLGERKSEGEVVDDLLSSYINLSGRCKLNAFRAENGGDSSDNEATSSVIGGANSMQRLDICSITDKKEGIKRSAGGDIASTRHYRSVSMDSVIGKMNFVDESVKLLPSTRGQIGTFPSSDTVDANSDTFSLDFGNGVFNEAELKKIMASENLAEMALTDPKRVKRILANRQSAARSKERKMRYIAELETKVQTLQNEATTLFTQVTLLQRELTSLTSQNNELKFRLQAMEEQAHLRDALNDTLTAEVERLKLMRMELSENGASFSQLSINPQMFQTQLQSQQQTDGST</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GFC2</accession>
    <name>A0AAD5GFC2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_006745</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008457">
        <property type="protein sequence ID" value="KAI7740335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GFC2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052462">
        <property type="entry name" value="SLIRP/GR-RBP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48027">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN 87F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="45"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="125"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="135"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7740335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="145" mass="15674" checksum="39854EBB3390A9E5" modified="2024-05-29" version="1">MAACRRISKTIFSNINIISRSSTGYQSSSSFGPPSSFITRRGIASRLYVAGLSFYTTEKALLDAFSQYGQVVEANVLMDKVSSRSKGFGFVTYASAEEAEKAIAEMDGKTLHGRIITVELAKPRTKNTGGMPIARGPPEPPTENQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GFE1</accession>
    <name>A0AAD5GFE1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Translation elongation factor EF1B beta/delta subunit guanine nucleotide exchange domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_017153</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">EF-1 is composed of 4 subunits: alpha, beta (1B-alpha=beta'), delta (1B-beta), and gamma (1B-gamma).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the EF-1-beta/EF-1-delta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008444">
        <property type="protein sequence ID" value="KAI7740395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GFE1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005853">
        <property type="term" value="C:eukaryotic translation elongation factor 1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00292">
        <property type="entry name" value="EF1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.60:FF:000001">
        <property type="entry name" value="Elongation factor 1-beta 1 like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.130:FF:000006">
        <property type="entry name" value="Elongation factor 1-delta 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036219">
        <property type="entry name" value="eEF-1beta-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049720">
        <property type="entry name" value="EF1B_bsu/dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014038">
        <property type="entry name" value="EF1B_bsu/dsu_GNE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014717">
        <property type="entry name" value="Transl_elong_EF1B/ribsomal_bS6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11595">
        <property type="entry name" value="EF-HAND AND COILED-COIL DOMAIN-CONTAINING FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11595:SF21">
        <property type="entry name" value="ELONGATION FACTOR 1-BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00736">
        <property type="entry name" value="EF1_GNE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54984">
        <property type="entry name" value="eEF-1beta-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Translation elongation factor EF1B beta/delta subunit guanine nucleotide exchange" evidence="6">
        <location>
            <begin position="142"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="116"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="117"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7740395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="189" mass="20220" checksum="F500E2F9EFF40785" modified="2024-05-29" version="1">MAPTFYVGSEAGLKKLDEYLLTRSYITGYQASKDDLTVHAAFTKAPSAQYVNVSRWFNHIEALLRLSGVSAEGSGVVVEGSASFPEEAVAAPVADAKAAADEDDDSDVDLFGEETEEEKKAAEDRAAAVKASGKKKESGKSSVLMDVKPWDDETDMKKLEEAVRSVQLEGLLWGASKLVPVGLGMNLVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GG44</accession>
    <name>A0AAD5GG44_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PRONE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_026371</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008514">
        <property type="protein sequence ID" value="KAI7740094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GG44"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.2010:FF:000001">
        <property type="entry name" value="Rop guanine nucleotide exchange factor 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2010">
        <property type="entry name" value="PRONE domain, subdomain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005512">
        <property type="entry name" value="PRONE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038937">
        <property type="entry name" value="RopGEF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33101">
        <property type="entry name" value="ROP GUANINE NUCLEOTIDE EXCHANGE FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33101:SF2">
        <property type="entry name" value="ROP GUANINE NUCLEOTIDE EXCHANGE FACTOR 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03759">
        <property type="entry name" value="PRONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51334">
        <property type="entry name" value="PRONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRONE" evidence="4">
        <location>
            <begin position="77"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="19"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="465"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="32"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="53"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="470"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="488"/>
            <end position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7740094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="499" mass="55676" checksum="AC6E7555872DC0A4" modified="2024-05-29" version="1">MTYNGLESCILNANSYDDESVASKGDGCPTDSPSSSCSSNNPSGSSFSQWSMKKRDEHGLDEWDHSDDPQQFITKENSSYTVKYADVEGMKEKFSKLLLGEDATGGRTVFGELWKLEPLPEDKRNKWRKEMDWLLSPTNYMIMTPKARVDVHLNLPALQKLDSMLLETLDSMTETEFWYEEGGSRAEGRDGNLKQSKRWWLPAPQVPVGGLSDGERKKLLIRAKLVLQIFKAAKSINEAILLEMPIPKIISDALPKSGKASLGDDLYRVLTRVSSASTMLESLSLKSENSALDVINRLDAAIHAWKDRIEEHGSGKSPARTSWSFRDPVSEHDKTELLINRAENLSREIKIRYPNLPQKFLDVMKIQYGKDVAHAIMEAYSRVLTNLAFSILTRIGDISQEDVLSDANSPMAKNTLLGVNIPCILDQSSSNIKARRTLIDKMDGIERKLSLLKTEEASDTALLRDESNRRSVTATPSTSSGCVGKESCFSSPADTPRSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GGD2</accession>
    <name>A0AAD5GGD2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_006158</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008172">
        <property type="protein sequence ID" value="KAI7741550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GGD2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7741550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="126" mass="14106" checksum="B81288B90D29970D" modified="2024-05-29" version="1" fragment="single">ESSRRGSNLRNLSSSSSVFFSANQSPFPSPRSSFWQPANDGHVQAATSTSHNLLPYTTCHENEAFDHIEKSKKVLKSPLSSFTPPSTSYCPTRLRNFDVYVGFHGKKALLLRFIERNVATQENITL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GIC7</accession>
    <name>A0AAD5GIC7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_026966</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007666">
        <property type="protein sequence ID" value="KAI7743830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GIC7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7743830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="88" mass="9503" checksum="5884986C83121BEC" modified="2024-05-29" version="1">MTSVGAQRLETVAPPFKWGANDLLEIGSLKASESGMGRVANDTYLAFMGNATTEGERDNLLLAAKKIRKMFQHCDACSLDTSFSEADT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GIM0</accession>
    <name>A0AAD5GIM0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">M8C21_028160</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">AA19_3_7</strain>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010007581">
        <property type="protein sequence ID" value="KAI7744130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GIM0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056760">
        <property type="entry name" value="RING_XB3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24161">
        <property type="entry name" value="ANK_REP_REGION DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24161:SF85">
        <property type="entry name" value="PALMITOYLTRANSFERASE HIP14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24921">
        <property type="entry name" value="RING_XB3-XBAT31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10 11" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="repeat" description="ANK" evidence="11">
        <location>
            <begin position="44"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="11">
        <location>
            <begin position="77"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="11">
        <location>
            <begin position="111"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="11">
        <location>
            <begin position="174"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="11">
        <location>
            <begin position="217"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="14">
        <location>
            <begin position="313"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="441"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="463"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="15">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAI7744130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="482" mass="52155" checksum="FA5BF7FDFF57D8AE" modified="2024-05-29" version="1" fragment="single">MGNSFGCSATGERLVSAARDGDVQEAKALLEYNPRLARYSTFGVRNSPLHYSAAQGHHEIVSLLIQSGVDINLRNYRGQTALMQACQYGHWEVVLTLVLYKANIHKADYLNGGTALHMAAFSGHTRCIRILLADYIPSVPNFYNFIIKRSRVKESGLEFDEGSLIDTINRPADGGLTALHMAALNGHVDSLQLLIDLGASVNKVTVEDGTTIDLIGAGSTPLHYAACGGNAQCCQILIARGASITAENAKGWTPLAVARLWHREWLDEVLIALPQDGEPTSSPYWCLPLMSVVKIARVCGWSNDSISTCADPCAVCLENKCALAADGCHHEFCTRCALYLCSTSTSSNASQGPPGSIPCPLCRQGIISFKKLPGTRPLLGMPRTSLPLSFFTCTSLTEDTDNTTLDTTPLCKPEFPYSLSSLSCQKFPSMKLSSGLCMGTSDTGPSLSRPSVDRGRFSRSSFRRSDSHSDSRRSWLCSLNQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GIQ2</accession>
    <name>A0AAD5GIQ2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_000590</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="JAMZMK010008134">
        <property type="protein sequence ID" value="KAI7741746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GIQ2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF213">
        <property type="entry name" value="3'-5' RNA HELICASE YTHDC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase-associated" evidence="4">
        <location>
            <begin position="14"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="403"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="403"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="424"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7741746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="443" mass="47869" checksum="D67BF42F590888A2" modified="2024-05-29" version="1" fragment="single">KKTLDPPVSQAIYNAITVLEDVGALSPNEELTELGEKLGSIPVHPVTSKMLLFAISMNCLDPALTLACASNFRDPFTLPMSPDDKVKANAAKSELASLYGSHGDQLAIIAAFECWKNAKQSGNVARFCSQYFVSPGVMRMLYAMREQLKSELHMNGFIPENSRFSENSQDGGIINAVLVAGLYPMVGRLFLPTPIVKRCVIKDASDQRVCVGRQSVNSRLTLKKKDVVPLVIYNEVTRGDAGLSIKNCSIIGPLPLLLLATEIAVAPINDDNETICKVNDEDGNKERVKEKFMSNPDTMVEVIADRWLSFKSTALDAAQICCLRERLSEAILFKIAHPGKDLPRVLAASVVAIAKVLSSDGISGINEYFESADLSTSVVAETDLGQQGKNGAIAGSVGSLRSLLSNGSESQPQHPSNVAPENIHPNHDAPRKGSLKRPREDTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GJ68</accession>
    <name>A0AAD5GJ68_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Patatin</fullName>
            <ecNumber evidence="7">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_016417</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Lipolytic acyl hydrolase (LAH).</text>
    </comment>
    <comment type="domain">
        <text evidence="7">The nitrogen atoms of the two glycine residues in the GGXR motif define the oxyanion hole, and stabilize the oxyanion that forms during the nucleophilic attack by the catalytic serine during substrate cleavage.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the patatin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="7"/>
    <dbReference type="EMBL" id="JAMZMK010007441">
        <property type="protein sequence ID" value="KAI7744790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GJ68"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047372">
        <property type="term" value="F:monoacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004620">
        <property type="term" value="F:phospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07214">
        <property type="entry name" value="Pat17_isozyme_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1090.10:FF:000005">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32176:SF80">
        <property type="entry name" value="ACYL TRANSFERASE_ACYL HYDROLASE_LYSOPHOSPHOLIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32176">
        <property type="entry name" value="XYLOSE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 7" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="5 7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="3" id="KW-0611">Plant defense</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PNPLA" evidence="8">
        <location>
            <begin position="23"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXGXXG" evidence="6">
        <location>
            <begin position="27"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DGA/G" evidence="6">
        <location>
            <begin position="202"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7744790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="428" mass="47162" checksum="4BFDCB3EF805F24D" modified="2024-05-29" version="1">MSFKEEPRSPLQPPTYGNLITILSIDGGGVRGIIPSVILEFLECELQVQLMSIGGLVTAMLTAPNEEGRPLFAAKDLKTFYLEHCPKIFPHDDNLLAPVAKVIKALSGPKYDGEHLHKVTQEKLQEKRLHETLTNIVIPTFDIKCLQPTIFSSYQLKKHPSLDAKLSDICIGTSAAPTYLPSHSFQTKDSEGKLLKEFNLIDGGVAANNPTLVAISEVTKEITTGSPDFFPIKPMDYGRFLVLSLGTGSAEFHVKYDSTKSSSWGVLGWLTGGGSTPLVDVFTQASDDMVDYHISTVFQALHSQENYLRIQDDTLSGDLASMDLATQENLHNLVSVGEKLLKKPVTRVNLGTGICEPYHDTTNEMALKKFAAILHKEKNVRELRSPNTNQGWANQGKRIKDPTTSLAMSIPSETIHHSLPHLHELNLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GJB4</accession>
    <name>A0AAD5GJB4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">5'-nucleotidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_014946</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 5'(3')-deoxyribonucleotidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008129">
        <property type="protein sequence ID" value="KAI7741853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GJB4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008253">
        <property type="term" value="F:5'-nucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008380">
        <property type="entry name" value="HAD-SF_hydro_IG_5-nucl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12103">
        <property type="entry name" value="5'-NUCLEOTIDASE DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12103:SF15">
        <property type="entry name" value="CYTOSOLIC PURINE 5'-NUCLEOTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05761">
        <property type="entry name" value="5_nucleotid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7741853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="193" mass="21852" checksum="903DEAF1A505727A" modified="2024-05-29" version="1">MDAISYGFASDIKGPNGCGKTEVSCVWASPEGGQKIEIGKHIFCNRAVNMKNIVAVGFDMDYTLAQYKPETFESLAYSGTVKKLVANLGYPNELLDWSFDWTYMVRGLVLDKKRGNILKMDRHKYVKVAYHGFRLLSKDEKVATYGNTLVRDAFDEPDYALIDTLFSLAEAYLFAQLVDFKDNNPEKISEEKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GK14</accession>
    <name>A0AAD5GK14_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Citrate transporter-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_027711</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the NhaD Na(+)/H(+) (TC 2.A.62) antiporter family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007143">
        <property type="protein sequence ID" value="KAI7745765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GK14"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004680">
        <property type="entry name" value="Cit_transptr-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045016">
        <property type="entry name" value="NhaD-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF038006">
        <property type="entry name" value="NhaD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43269">
        <property type="entry name" value="SODIUM/PROTON ANTIPORTER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43269:SF2">
        <property type="entry name" value="SODIUM_PROTON ANTIPORTER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03600">
        <property type="entry name" value="CitMHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0050">Antiport</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0915">Sodium</keyword>
    <keyword evidence="9" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="233"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="273"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="311"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="357"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="381"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="422"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="461"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="496"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="533"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="domain" description="Citrate transporter-like" evidence="13">
        <location>
            <begin position="147"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="53"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF03600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7745765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="571" mass="60654" checksum="7B3030314CBF9ACF" modified="2024-05-29" version="1">MSTTLLPFKTLLNPQLRSRNYVVSSPLLTSRPPKLSLSLLFQRHDEVLARAEDKARAGRGGEQPNSSKPSSDKQIQDLTSPGACDPLCSVDETSSLEYEATYQPKTDLLKALAIFGAAATGAVAINHSWVAANQDLAMALLFGIGYAGIIFEESLAFNKSGVGLLMAVSLWVIRSIGAPSTDIAVAELTHASAEVSEIVFFLLGAMTIVEIVDAHQGFKLVTDNITTRKPRSLLWVVGFVTFFLSSVLDNLTSTIVMVSLLRKLVPPSEYRKLLGAVVVIAANAGGAWTPIGDVTTTMLWIHGQISTLPTMTSLFVPSVVSLAVPLALMSFSSEVNGKGQNTDDVLASEQMAPRGQLVFAVGIGALVFVPIFKSLTGLPPYLGMLFGLGVLWILTDAIHYGESERQQLKVPQALSRIDTQGVLFFLGILLSVSSLEAAGLLREIANYLDAHIPSSELIASAIGVVSAIIDNVPLVAAAMGMYDLSSYPQDSQFWQLVAFCAGTGGSMLVIGSAAGVALMGMEKIDFFWYLRKASGFAFAGYAAGIAAYLASQNLHLSPTTIAANIPFISGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GM34</accession>
    <name>A0AAD5GM34_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_028551</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006614">
        <property type="protein sequence ID" value="KAI7748047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GM34"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021920">
        <property type="entry name" value="DUF3531"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46737">
        <property type="entry name" value="OS02G0827600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46737:SF2">
        <property type="entry name" value="OS02G0827600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12049">
        <property type="entry name" value="DUF3531"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7748047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="283" mass="32295" checksum="F8129E72EDC6C7A2" modified="2024-05-29" version="1">MHISSAFNYSSSISLNPKYLHLFPKFVLRNVRTTITAKSSNEDDDVRGSGTTARGRRLLKVREEKRKREYDRLHNYPSWAKVLEDAAKNDVELRNVLGDSIGKPEQMRQKVEDRIRKKGRDFHKAKTGSVVAFKVTFRDFSPVGSNIWFKLYGPPSDRDVDLIGSVIQSWYVLGRLGAYNSSNLQLANASMEYNPLYDADKGFSVMPSSFHDIGDVEFQDNWGRVWVDIGTSDYFALDVLLNCLTVLSSEYLGIQQVVFGGRSMGDWEEGMKNPEDGYKYFKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GN46</accession>
    <name>A0AAD5GN46_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_032806</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006451">
        <property type="protein sequence ID" value="KAI7748885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GN46"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7748885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="78" mass="8555" checksum="59730A4707EFFAA2" modified="2024-05-29" version="1" fragment="single">GFASIVVRLFKKASLDDTANKDDEEEAIGGSNNLALQKRLAKGCNGANMTRGQRISRAYDVIQQSVNISGTLKKMKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GNK3</accession>
    <name>A0AAD5GNK3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_026526</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006378">
        <property type="protein sequence ID" value="KAI7749380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GNK3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009737">
        <property type="term" value="P:response to abscisic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057059">
        <property type="entry name" value="LTI65/LTI78_PGEED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056605">
        <property type="entry name" value="LTI65_LTI78_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037491">
        <property type="entry name" value="LTI78/LTI65"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33836">
        <property type="entry name" value="LOW-TEMPERATURE-INDUCED 65 KDA PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33836:SF7">
        <property type="entry name" value="LOW-TEMPERATURE-INDUCED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23403">
        <property type="entry name" value="LTI65_LTI78_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23399">
        <property type="entry name" value="LTI65_PGEED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LTI65/LTI78 N-terminal" evidence="3">
        <location>
            <begin position="65"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="LTI65/LTI78 PGEED repeat" evidence="2">
        <location>
            <begin position="184"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="233"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="19"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="45"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="158"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="264"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7749380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="302" mass="32324" checksum="907F3F8E61113B4C" modified="2024-05-29" version="1">MAQQDDTKTTTNASNAPRSQDSSGTKSSTTQTSVDVQHLQGGGTSSSPKCSSKAKPNHDHDDHGNHHHKKSVFTKVKEKARKLKKSISGRKRREENGTQDPYITPPGSAGSLDDDKDQPEYFGSPLYEARDVPNTDTQSGKQPENATPSLDSDDTNADSKQTGLTVSTHEDTEIAKADASSTQDKGLSVKEYIMHTFEPGEDERALSQAITQTISPKCKRVKEAVNSLLGNEEASDLISKNTNVEHDVTPSSSLTDSNDKKLTSLANDPNLSNALEPSLSNASNISLCSTPTSASLQNPNSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GR70</accession>
    <name>A0AAD5GR70_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box/LRR-repeat protein 15/At3g58940/PEG3-like LRR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_021913</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006341">
        <property type="protein sequence ID" value="KAI7749546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GR70"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055411">
        <property type="entry name" value="LRR_FXL15/At3g58940/PEG3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31639">
        <property type="entry name" value="F-BOX PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31639:SF315">
        <property type="entry name" value="LEUCINE-RICH REPEAT DOMAIN SUPERFAMILY, F-BOX-LIKE DOMAIN SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24758">
        <property type="entry name" value="LRR_At5g56370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box/LRR-repeat protein 15/At3g58940/PEG3-like LRR" evidence="1">
        <location>
            <begin position="114"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF24758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7749546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="295" mass="34095" checksum="5BA724E5BAE2DACB" modified="2024-05-29" version="1" fragment="single">MDRISKLPVGVIETILCLLPIHEAARTSILSREWRYRWITIPKLVFNEDMFEVSDYVSEPSVLEQTFDKPSHRKVMTTRCKLFYAIYQVLFMHEGPIHEFTLSMEVDRSCVEIDHIIRHLSKKNTVKILKLELKWVYKLPLSLFSLHKLTGLYLSGCVLDHQPSSNVFRSLTTLYLDNIRTCKKELLCLVSSCPSLKRLTILSDAGTINDGGDYTIADLIKCVPLIEYLSLMFFIFLINVDEEFDESVMGPFIPEECSDILLERLNDLEILHFCNEENELAFVKLILAKSPVLKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-02-05" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GSD9</accession>
    <name>A0AAD5GSD9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">OBG-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_001180</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005492">
        <property type="protein sequence ID" value="KAI7753265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01897">
        <property type="entry name" value="NOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1190:FF:000001">
        <property type="entry name" value="Nucleolar GTP-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000496">
        <property type="entry name" value="Nucleolar GTP-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041623">
        <property type="entry name" value="NOG1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010674">
        <property type="entry name" value="NOG1_Rossman_fold_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012973">
        <property type="entry name" value="NOG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45759">
        <property type="entry name" value="NUCLEOLAR GTP-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06858">
        <property type="entry name" value="NOG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17835">
        <property type="entry name" value="NOG1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08155">
        <property type="entry name" value="NOGCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="OBG-type G" evidence="7">
        <location>
            <begin position="169"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="394"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7753265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="481" mass="54852" checksum="4B2FFC4B53FD255A" modified="2024-05-29" version="1">MVQYNFKKITKVPTGKDFVDIVLSRTQRQTPTVVHKGYAINRIRQFYMRKVKFTQTNFHEKLSTIIEEFPRLDDIHPFYGDLLHVLYNKDHYKLALGQINTARNLISKIAKDYVKLLKYGDSLYRCKALKVAALGRMCTVTKRIGPSLAYLDQIRQHMARLPSIDPNTRTILICGYPNVGKSSFINKITRADVDVQPYAFTTKSLFVGHTDYKYLRYQVIDTPGILDRPFEDRNIIEMCSITALAHLRAAVLFFLDISGSCGYTIAQQSALFHSIKSLFMNKPLIIVCNKTDLQSLEGISSDDKKLVEEMKSEAMKTLIGQGGEPREGAEVLLTMSTLTEEGVIAVKNAACERLLDQRVEVKMKSKKINDCLNRFHVAVPKPRDQKERPPCIPQAVLEAKAKEAEKEKRKLERDIEEENGGAGVYSASLKKHYLLANDEWKEDNMPEILDGHNVYDFVDPDILQRLEELEKEXXXXGAGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GSP2</accession>
    <name>A0AAD5GSP2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_031383</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006112">
        <property type="protein sequence ID" value="KAI7750628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GSP2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7750628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="119" mass="13195" checksum="54C884422A6D2E8D" modified="2024-05-29" version="1">MNPSILVGKQRGKRLLGIQGSPWHGFDQACLCRCRPRDQKVVMCSAISGGSLCRSHGGFAYYYSKDQQFHSGGVTGTIADHIISTSFFYEDKTSLLQTVASTSNFICGFEDEGLFFYVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GT05</accession>
    <name>A0AAD5GT05_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-CH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_024803</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010003901">
        <property type="protein sequence ID" value="KAI7754287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GT05"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16495">
        <property type="entry name" value="RING_CH-C4HC3_MARCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000337">
        <property type="entry name" value="Zinc finger family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022143">
        <property type="entry name" value="DUF3675"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033275">
        <property type="entry name" value="MARCH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23012:SF189">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE MARCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23012">
        <property type="entry name" value="RING/FYVE/PHD ZINC FINGER DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12428">
        <property type="entry name" value="DUF3675"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12906">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51292">
        <property type="entry name" value="ZF_RING_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="166"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="197"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="RING-CH-type" evidence="5">
        <location>
            <begin position="45"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7754287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="252" mass="28197" checksum="4408DDFC1D294C56" modified="2024-05-29" version="1">MGDHFVFLVDRLLTESTLEAAIESTKPSYSVNETVVDCSSVDAVYGSGKLVECRICQDEDFDSNMETPCSCSGSLKYAHRRCVQRWCNEKGDIICEICHQQFMPGYTAPPPVFRLGDIPASFRGHWQISRRDMNNNHRIITVVSSDHNFLDQEYDEYAESTARSILCFRSAAIIFMILLILRHTLPMFASGVGNYSLPAFMLLLIRTSGIVVPIYIILRAFTALLHRRHQLASNSSTSSLSSSDEEAGPTPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GTM9</accession>
    <name>A0AAD5GTM9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_032643</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010001760">
        <property type="protein sequence ID" value="KAI7755082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GTM9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7755082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="115" mass="13350" checksum="AF50AE46D41A2BE2" modified="2024-05-29" version="1" fragment="single">FYAFCVPRIRNEFLFEPQRWSTNYTAVSVENSYTRSCIISGVVNYGGDNTSIRYKGGITWWCKGDAFAVMGSRGCFFDGYKMGSYTWLVLIRRIDAKETGWRLRRLLRTKEDTGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GV40</accession>
    <name>A0AAD5GV40_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Non-specific serine/threonine protein kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_014463</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000259">
        <property type="protein sequence ID" value="KAI7756792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GV40"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050517">
        <property type="entry name" value="DDR_Repair_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046807">
        <property type="entry name" value="Tra1_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046805">
        <property type="entry name" value="Tra1_ring"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11139">
        <property type="entry name" value="ATAXIA TELANGIECTASIA MUTATED ATM -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11139:SF1">
        <property type="entry name" value="TRANSFORMATION_TRANSCRIPTION DOMAIN-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20175">
        <property type="entry name" value="Tra1_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20206">
        <property type="entry name" value="Tra1_ring"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="402"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="45"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7756792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="860" mass="95685" checksum="F1E60732AE04A964" modified="2024-05-29" version="1">MPTQETEVQPFLDFVCKIYQNFRLTVSHFFESGAVVAPAMPPPSSMSGLPSLGSEDVKPMEMPDQVGSSTPSSTGAGQLNPSTRSFKIVTESPLVVMFLFQLYNRLVQTNIPQLLPLMVAAISVPGPDKVPPHLKTHFTELKGAQVKHLSTHALTVSFLTYLLKSFADYIRPHEESICKSIVNLLVTCSDSVSIRKELLIALKHVLGTDFKRGLFPLIDTLLEERVLVGIGRACFETLRPLAYSLLAEIVHHVRGDLSLSQLSRIIYLFSSNMHDASLSLSIHTTCARLMLNLVEPIFEKGVDLQSMDEARILLGRILDAFVGKFSTFKRTIPQLLEDVEEGKERSTLKLKLELPVQAVLNIQVPVEHSKEVSDCKHLIKTLVMGMKTIIWSITHAHLPRSQVSPSAHGTPSQMLPATPPNSSVGQPFKGLREEEVWRASGVLKSGVRCLALFKEKDEEREMIHLFSQILAIMEPRDLMDMFSLCMPELFECMVSNTQLVHIVSTLLQAPKVFRPFADVLVNFLVSSKLDALKHPDSPAAKLVLHLFRFLFGAVAKAPADCERILQPHVSVIVETCMKNASEVERPLGYLQLLRTMFRALAGGKFELLLRDLIPTLQPCLNMLLAMLEGPTAEEMRDLLLELCLTLPARLSSLLPHLPRLMKPLVMCLNGSDELVSLGLRTLEFWIDSLNPDFLEPSMANVMSEVILALWSHLRPAPYPWGGKSLQLLGKLGGRNRRFLKEPLALECKENPEHGLRLILTFEPETPFLVPLDRCINLAIAAVMNKNSSMDAFYRKQALKFLQVCLSSQLDLPGRVTDDGLISKHLSTVLVSSVDPSWGRSDASDVKVCLVTNSILNLDPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GV41</accession>
    <name>A0AAD5GV41_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_011594</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005586">
        <property type="protein sequence ID" value="KAI7752878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GV41"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47818:SF2">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47818">
        <property type="entry name" value="RNI-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042090786" evidence="1">
        <location>
            <begin position="17"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7752878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="173" mass="19560" checksum="86C5DBF52C2ED872" modified="2024-05-29" version="1">MWWKTAKLVFDTLLEAESCLQVLDLSENNITGWLSHFKWGSPSCSNTSRKISKSLKSLRVLNLRCNNLQKDDVDCLKYAMVYMPNLEVLNLSENPLQDEGIRNLFPYLVEKSKCETPLAELHLENCELSCDGVSQLLEVLAATNVPLKSLSIGDNHLTRLVPRELEVVKWPGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GV96</accession>
    <name>A0AAD5GV96_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF155 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_021415</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RMD1/sif2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005380">
        <property type="protein sequence ID" value="KAI7753581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GV96"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003734">
        <property type="entry name" value="DUF155"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051624">
        <property type="entry name" value="RMD1/Sad1-interacting"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16255:SF6">
        <property type="entry name" value="PROTEIN RETARDED ROOT GROWTH-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16255">
        <property type="entry name" value="REQUIRED FOR MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02582">
        <property type="entry name" value="DUF155"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="302"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="DUF155" evidence="3">
        <location>
            <begin position="105"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7753581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="330" mass="37878" checksum="81C1B3A60D270740" modified="2024-05-29" version="1">MDKRDAEIKEKLSSVKDTSAEVKQLVEQTTAVMRAEEGEEEDDSGDDVVDEDVKPSIPELMDQNRPNYIQPTSRMTNYVVLRFGSTKPESIGLGASLSGSDSVYMVVFQYGSVVLFNVREHESERYLKIVKEHASGELSEQEMRKDEYEVKEKPTLETWMEGGLDYIMLQYLNIDGIRTIGSVLGQSVALDYYVRQVDGMVGEFTDINRGMEKTGTFTMKRKKLFQLVGKANSNLADVILKLGLFERSDIAWKDAKYAQIWEYLRDEFELTQRFASLDFKLKFVEHNIRFLQEILQNRKSDFLEWLIIILIGAEILISVYDIAHKSVTSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GVP8</accession>
    <name>A0AAD5GVP8_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_025732</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010002311">
        <property type="protein sequence ID" value="KAI7754884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GVP8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44579">
        <property type="entry name" value="OS01G0730500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44579:SF2">
        <property type="entry name" value="OS01G0730500 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13370">
        <property type="entry name" value="Fer4_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="1">
        <location>
            <begin position="155"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="2">
        <location>
            <begin position="235"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7754884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="417" mass="46966" checksum="8F35C19068A9A9E6" modified="2024-05-29" version="1">MQPKSCSHCWNASTVVILPIQSAWKTAWEFTIFTRSDGGVACKIGTPTCRHVLEEGKIKVEGGSFKLMAVNGPSHQRREGGLKLEMAQLMSPVCTEGIKFQKAGGWRMRMLSNNNSILLGCKAPWRRRRRPLTHRLKVTTDPSPFSSSDAAAADDYYAVLGLLPDATPEQIKKAYYNCMKSCHPDLSGDDPETTNFCMFINEVLSDPAQRMVYDEIHGYALTAINPFHDDTYPKDHVFVDEFSCIGCKNCANVCSTVFMIEEDYGRARVCNQQGNPDLVQEAIDSCPVDCIHWTSAAQLSLLEDEMRRVERVNVALMLAGMGSSADVFRMASSRWEKRQNKILEQARIRMMKSNDKAEPYWTNLWGNAKNQPSTEEEVRERASRAAAAARRWREYSRRGVDKPATYKLPGNLSPTEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GWS5</accession>
    <name>A0AAD5GWS5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pentatricopeptide repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_026011</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010001697">
        <property type="protein sequence ID" value="KAI7755141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GWS5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099402">
        <property type="term" value="P:plant organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009451">
        <property type="term" value="P:RNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000158">
        <property type="entry name" value="pentatricopeptide repeat-containing protein At2g33680"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000682">
        <property type="entry name" value="Pentatricopeptide repeat-containing protein At3g16610"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046960">
        <property type="entry name" value="PPR_At4g14850-like_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47926:SF451">
        <property type="entry name" value="TETRATRICOPEPTIDE-LIKE HELICAL DOMAIN SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13812">
        <property type="entry name" value="PPR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="118"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="216"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="307"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="408"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7755141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="643" mass="71341" checksum="7E041729C00094B3" modified="2024-05-29" version="1" fragment="single">MKLLQVPNHPKPPSSLATKSHNALINRHSSTGNHRHVLLLYFTMLNAHIPPDPYTYPSMLKACTSLNLFSLGLSFHQHLLVNGYSSDSYISASLINFYSKFGFADVAYKVFDVMPDRNVVPWTAVIGCYTRYGDVNTAFRMYKQMQCEGVRPSSVTILTMVSGVSEYAHVQSFHACSIRYGFVGDLALANSVLNSYGKCRRVSDASNLFESMDKRDIVSWNSLVSAYALVGNVREISQLLYRMWLSELEPDQQTFGSLISAVTRQGNQYIGKVVHGKVVIPSTTTIACALAACAQLGSFRVGTSIHDVVSWNAMVAGHAQNGQLFNALYVFNEMRKSFEKPDAVTVVSLLQACASVGAHHQGKWIHNFIIRNSLESSLLIDTAIIDMYCKCGNIDSARKCFDRMSKHDVVTWSTMIGGYGSHGKGEDALDMFMEFLQSGLEPNHVIFLSVLYSCSHNGLVDEGLALFNTMKDRYKIEPKLEHCACIVDLLCRAGRTTDAYDFYKKTFMEPVVDVLGILLDACRSKGNKELGDVIASEIHRWYGTDGSVGWNWGVMVAAGSPAVSSDGLGKSNGDDTPFGKKIGAMEKVLERRQRRMIKNQESAARSHTRKQVEVKCENADSVLKHKQEVITKSTAQIIHEHNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GXD7</accession>
    <name>A0AAD5GXD7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Sugar phosphate transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_025998</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010001697">
        <property type="protein sequence ID" value="KAI7755128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GXD7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004853">
        <property type="entry name" value="Sugar_P_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050186">
        <property type="entry name" value="TPT_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11132">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03151">
        <property type="entry name" value="TPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="44"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="67"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="105"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="143"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="170"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="197"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="Sugar phosphate transporter" evidence="6">
        <location>
            <begin position="45"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7755128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="221" mass="23999" checksum="207DCF32B60C43AD" modified="2024-05-29" version="1">MCHMSACAILSYVSIVFLKIVPFQKIKSRSQFLRIATLSIREFWVTYCALIPVVAGVVIASGGEPSFHLYGFVMCISATAARAFKSVLQGILLSSEGEKLNSMNLLLYMSPVAILVLLPAALIMEPDVIDVTVSLALEHRFMWVLLLINSTMAYAANLTNFLVTKNTSALTLQVLGNAKGAVAVVISILLFRNPVTVVGIAGYSMTVMGVVAYGETKRRFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GYA1</accession>
    <name>A0AAD5GYA1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_001918</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000129">
        <property type="protein sequence ID" value="KAI7757389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GYA1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7757389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="63" mass="7461" checksum="40DCAD47736C3915" modified="2024-05-29" version="1" fragment="single">MDSSIVDTLQHSHHQHPLNLVYLQQPTHKKENVDDEDENEVEVEDEVEFVEEDRQCSLCEEQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GYA2</accession>
    <name>A0AAD5GYA2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ARC105/Med15 mediator subunit C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_009891</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000341">
        <property type="protein sequence ID" value="KAI7756581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GYA2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048386">
        <property type="entry name" value="Med15_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044661">
        <property type="entry name" value="MED15a/b/c-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33137">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 15A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33137:SF4">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 15A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21539">
        <property type="entry name" value="Med15_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ARC105/Med15 mediator subunit C-terminal" evidence="2">
        <location>
            <begin position="787"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="150"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="320"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="363"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="478"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="688"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="25"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="154"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="169"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="329"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="486"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="701"/>
            <end position="718"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF21539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7756581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="866" mass="96852" checksum="F727ADAAF45C359D" modified="2024-05-29" version="1">MGQQNKLSSVQQPQQQLIGQHNNLSSMQQQQSGKQQQLHGSQSDSTALTGNSNGGDWQEEVYRKIKAMKDLYLLDLTNMHQDTLGKLPQYDSLPQQPKNNQLDKLKMFKNMLERYIRLLQLPNKHNILANYKDKLGTYEKKIIQVINSHRRKAGGPQQQAQALPPAHMQSMQKSQQTHSQLTQVQSHDTQMNMQGSMAAMQPNNMGNLQQSTALSLSGGSNVQQNMMNSIQPTSNLESGQNTSMNSLHQVSSGSLQQNTVSGPQQVNINPKSLQPNTNMLQLQHIKQEQLLQSQQLKQFHQRQMQQQFLHKQQFMQQQQFHQLTKQQESNRQMQGNQLSQLHQKNDSGDIKLLQQMGVKSGAFQQQQLHGSQSDSTALTGNSNGGDWQEEVYRKIKAMKDLYLLDLTNMHQDILGKLPQYDSLPQQPKNDQLDKLKMFKSMLERYIQLLQLPNKHNILANYKDKLGTYEKKIIQVINSHRRKRRGPQQQAQALPPAHMQSMQQSHDTQMDIKGSIAAMQPNNMGNLQQSTAPSLSGGSNVQQNMMNSIQPTSNLESGQNTSMNSLHQVSSGSLQQTIVSGPQQININPMSLQPNTNMLQPQHIKQEQLLQSQQLKQFQQCQMQQQFEQVKSMLPKDLSASVSDIGSVVSMTDRITGSAPGNGSRAAVGEDLVAMTKCRLQARKFVTQDGTNGTRKMKRFTHLNTSETPELDLTASSTNKKPRTESNPLLEEIKEINRGLIDTVVSIPEEDVDPAAASAVSEGGDGTIITCSFSAVALGPNLKSQYASAQMSPIQPLRLFVPANYPNCSPILLDKFPAEVSKEFEDISTKTKSRFSVSLSSLQQPMSLKDIARTWDACAAAVMSEYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GYQ4</accession>
    <name>A0AAD5GYQ4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">glycine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_029397</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>tRNA(Gly) + glycine + ATP = glycyl-tRNA(Gly) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:16013"/>
            <dbReference type="Rhea" id="RHEA-COMP:9664"/>
            <dbReference type="Rhea" id="RHEA-COMP:9683"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78522"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.14"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.14" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010000371">
        <property type="protein sequence ID" value="KAI7756463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GYQ4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009570">
        <property type="term" value="C:chloroplast stroma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004820">
        <property type="term" value="F:glycine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006426">
        <property type="term" value="P:glycyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00733">
        <property type="entry name" value="GlyRS_alpha_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000006">
        <property type="entry name" value="Glycine--tRNA ligase alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.180:FF:000002">
        <property type="entry name" value="Glycine--tRNA ligase, chloroplastic/mitochondrial 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.180">
        <property type="entry name" value="Class II aaRS and biotin synthetases, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00254">
        <property type="entry name" value="Gly_tRNA_synth_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015944">
        <property type="entry name" value="Gly-tRNA-synth_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006194">
        <property type="entry name" value="Gly-tRNA-synth_heterodimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002310">
        <property type="entry name" value="Gly-tRNA_ligase_asu"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00388">
        <property type="entry name" value="glyQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00211">
        <property type="entry name" value="glyS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006827">
        <property type="entry name" value="PRK09348.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30075:SF2">
        <property type="entry name" value="GLYCINE--TRNA LIGASE, CHLOROPLASTIC_MITOCHONDRIAL 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30075">
        <property type="entry name" value="GLYCYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02091">
        <property type="entry name" value="tRNA-synt_2e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02092">
        <property type="entry name" value="tRNA_synt_2f"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01044">
        <property type="entry name" value="TRNASYNTHGA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50861">
        <property type="entry name" value="AA_TRNA_LIGASE_II_GLYAB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="938"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7756463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="938" mass="104661" checksum="DF9AE6056A7777FA" modified="2024-05-29" version="1" fragment="single">FYYCHGSKLKLLGPLSSYKNPIKTHTPFTLHNPKTMGILTLPLITSLLKPHKLPLSLLFTTTIHHHHRPVRTPFSRRTIPLCSFTNSATDPSPLTADVTNSNAEDRPIKVSIPTFQQAIQRLQEYWASVGCTIMQCSNTEVGAGTMNPLTFLRVLGPEPWNVAYAEPSIRPDDSRYGENPNRLQRHTQFQVILKPDPGNSQDLFIRSLSALGIDVNAHDIRFVEDNWESPVLGAWGLGWEIWMDGMEITQFTYFQQAGSLQLMPVSVEITYGLERILMLLQGVDHFKKIKYADGITYGELFSENEKEMSAYYLEHASVDHIQKHFDFFEAEARSLLDLGLAIPAYDQLLKTSHAFNILDSRGFVGVTERARYFGRMRSLARQCAMLWSNTRESLGHPLGIISQVNSLVVPKELLESEATKVSAEPRMFLLEIGTEELPPNDVASAGQQLKDLIVQLLGKQRLTHGEVHVFGTPRRLVISVENLCSKQSSNEVEVRGPPASKAFDNLGNPTKAAEGFCRRNSVPVDSLYRKTEGKTEYVYVRVMESAQLALEVLSKELPGAIAKVSFPKSMRWNSEVIFSRPIRWILALHGDAVVPFAFAGVDSAESYANVMQQAGISVDTEQRKKIILERANDLAKGVDGCLVMQSNLLDEVVNLVEAPVPVLGKFSESFLVLPKDLLVMVMQKHQKYFALTDNNGSLLPYFIAVANGAINESVVRKGNEAVLRARYEDAKFFYELDTSKRFSEFRGQLNGILFHEKLGTMEDKMIRVESTVSELGLALGLSEDKLQIAREAASLALSDLSTAVVTEFTSLSGIMARHYALREGYSQQVSEALFEITLPRFSGDILPETDAGIVLSIADRLDSLVGLFGAGCQPSSTNDPFGLRRISYGLVQVLVEKDRSFDLKRALEVAASVQSLKIDPVTLDEVHQFVTRRLEQFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GZ83</accession>
    <name>A0AAD5GZ83_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_019753</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000101">
        <property type="protein sequence ID" value="KAI7757556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GZ83"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01959">
        <property type="entry name" value="nsLTP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.110.10">
        <property type="entry name" value="Plant lipid-transfer and hydrophobic proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036312">
        <property type="entry name" value="Bifun_inhib/LTP/seed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016140">
        <property type="entry name" value="Bifunc_inhib/LTP/seed_store"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033872">
        <property type="entry name" value="nsLTP2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33214">
        <property type="entry name" value="BIFUNCTIONAL INHIBITOR/LIPID-TRANSFER PROTEIN/SEED STORAGE 2S ALBUMIN SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33214:SF51">
        <property type="entry name" value="BIFUNCTIONAL INHIBITOR_PLANT LIPID TRANSFER PROTEIN_SEED STORAGE HELICAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00234">
        <property type="entry name" value="Tryp_alpha_amyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00499">
        <property type="entry name" value="AAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47699">
        <property type="entry name" value="Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042154007" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein" evidence="3">
        <location>
            <begin position="28"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical" evidence="4">
        <location>
            <begin position="30"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7757556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="96" mass="10002" checksum="725AE0D479B14CA5" modified="2024-05-29" version="1">MKKLLAVIALQLLVAAVLVVGATPSTAVTCVPTQLAPCLKPIMNGSPPSSKCCSKLKEQKPCMCGYIKNPNFAKYVKSPGALKVAKACGVTIPKCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BKE4</accession>
    <name>A0AAD5BKE4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Polygalacturonase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_001484</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the glycosyl hydrolase 28 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012169">
        <property type="protein sequence ID" value="KAI7724756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004650">
        <property type="term" value="F:polygalacturonase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.10:FF:000012">
        <property type="entry name" value="Polygalacturonase At1g48100 family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000743">
        <property type="entry name" value="Glyco_hydro_28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31375">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00295">
        <property type="entry name" value="Glyco_hydro_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00710">
        <property type="entry name" value="PbH1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00502">
        <property type="entry name" value="POLYGALACTURONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0134">Cell wall</keyword>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6 9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041996920" description="Polygalacturonase" evidence="10">
        <location>
            <begin position="25"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7724756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="443" mass="48298" checksum="19BF4F946E6650B3" modified="2024-05-29" version="1">MGLSFYTCVLVFLTLYIHCRTIDSRSLDHISLPPSSAPEPSPSPKIQLSPGVFDVRSFGSIGDGVTDDTEGFKMAWDAACQSNEPSILLVPKHYSFLIQSTIFTGSCGNRVVFQIEGTIMAPDGPSVWPKGMSKRQWLVFYRVNGMSLQGGGVIDGRGEKWWDLPCKPHKGPNGTTLAGPCDSPVTIRFFMSTNVTVKGIKVKNSPQFNFRFDXXXXNIKAPSLSPNTDGIHIENTNDVTIYNSLISNGDDCVSIGAGSYNVDIKNITCGPSHGISIGSLGMRNTRACVSNITVTDSMIKHSDNGVRIKTWQGGFGSVSKVKFYNIHMDNVRNPIMIDQYYCQKKGMCPNQTAAVRISDVVYEKIRGTYDARSPAMHLGCSNSVPCTNLTFSDVELVPSQGLIMSSPFCWNAYGALQTLTIPPISCLLEGNPDSIPEVEVDQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BLN0</accession>
    <name>A0AAD5BLN0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_021482</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in sporophytic self-incompatibility system (the inability of flowering plants to achieve self-fertilization).</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012172">
        <property type="protein sequence ID" value="KAI7724671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BLN0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048544">
        <property type="term" value="P:recognition of pollen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00028">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01098">
        <property type="entry name" value="PAN_AP_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.90.10.10:FF:000005">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.4.10:FF:000002">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.90.10.10">
        <property type="entry name" value="Bulb-type lectin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.4.10">
        <property type="entry name" value="Hepatocyte Growth Factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001480">
        <property type="entry name" value="Bulb-type_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036426">
        <property type="entry name" value="Bulb-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000858">
        <property type="entry name" value="S_locus_glycoprot_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035446">
        <property type="entry name" value="SLSG/EP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32444">
        <property type="entry name" value="BULB-TYPE LECTIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32444:SF98">
        <property type="entry name" value="RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01453">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08276">
        <property type="entry name" value="PAN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00954">
        <property type="entry name" value="S_locus_glycop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002686">
        <property type="entry name" value="SLG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00108">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00473">
        <property type="entry name" value="PAN_AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51110">
        <property type="entry name" value="alpha-D-mannose-specific plant lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50927">
        <property type="entry name" value="BULB_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50948">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Bulb-type lectin" evidence="7">
        <location>
            <begin position="30"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="8">
        <location>
            <begin position="349"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7724671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="489" mass="55589" checksum="070DC113EF223A62" modified="2024-05-29" version="1">MEERKVANNKLIFAFLVVLHTSVTYIYAANSTLSANQTIRYNETIVSPKETFELGFFNLGNSSNYYVGIWYKKISKQTVVWVANRDAPLTNTSGELTLTLLGVLILRDATTGKILWSSANSSRTSVMNPIGQLLDTGNFIIYEDGEDINQENPIWQSFDFLTDTFLPGMKYGWDLVTGVEWRLTSWKSYDDPSSGNFSYGIDKRGYPQPILTDGPNIKFRTGPWNGLRYTGLPNLRPNPIYNFTFVHNQREIYYQNELINTSVLMRLVLQPDGRMERFLWTDSKQQWNLFQALDSDRCDRYALCGPFGRCNIANSSVCECLEGFEPISPDEWKGANWSRGCQRTIPLDCGPEDGFNKYSNLKLPDTKGSWYNQSMTLVDCEKMCKNNCSCTAYTNSDISVTGSGCLLWFGDLIDIRKFAINGDTLYIRMRPSEIGISIHEFEHDLENRSGERDLELPLFGLSTLHKATNNFSMNNKLGEGGFGPVYKDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BM27</accession>
    <name>A0AAD5BM27_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_003794</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011854">
        <property type="protein sequence ID" value="KAI7725805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BM27"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7725805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="144" mass="16490" checksum="B0EB9C771C92D86E" modified="2024-05-29" version="1" fragment="single">RRKNAEVVAPSLVSVRRSSRLKARSSVMSPQTESSHTIKKHVNSITSQLIIWDKDRIQKALRELLLRWVQTRPSYAAISGQPEPHQSTMPRLKLPMLKHSSMSRIRLAKSNEVLPWSTASNCQMRFHEKIFNMADVIVSPMVGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BP72</accession>
    <name>A0AAD5BP72_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Thaumatin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_007885</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the thaumatin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011457">
        <property type="protein sequence ID" value="KAI7727053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BP72"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.110.10:FF:000003">
        <property type="entry name" value="Thaumatin I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.110.10">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037176">
        <property type="entry name" value="Osmotin/thaumatin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001938">
        <property type="entry name" value="Thaumatin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017949">
        <property type="entry name" value="Thaumatin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31048">
        <property type="entry name" value="OS03G0233200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00314">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00347">
        <property type="entry name" value="THAUMATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00205">
        <property type="entry name" value="THN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49870">
        <property type="entry name" value="Osmotin, thaumatin-like protein"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00316">
        <property type="entry name" value="THAUMATIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51367">
        <property type="entry name" value="THAUMATIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042175229" description="Thaumatin-like protein" evidence="3">
        <location>
            <begin position="22"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7727053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="505" mass="54237" checksum="DC073C7347F4B286" modified="2024-05-29" version="1" fragment="single">MTVSTISTFLLLAILSCSTNAAVFTILNNCPYTIWVGAVPGGGQQLNSGQTWTLSVPSGTSGRIWPRTNCVFDGSGRGKCQTGDCNGLLRCQNYGTPPNTLAEYALNQVNNLDFFDMSLVDGFNVPMEFKPNSGGCTRGILCKSDINGQCPNELRAPGGCNNPCTVFKTDQYCCNSGNCGPTDFSKFFKTRCPDAYSYSRDDQTSIFTCPGGTNFLLLALLFHSTNAAVFTIRNNCPYTVWAGAVPGGGQQLGPGQTWPLTVAAGTQGARIWPRTGCNFDGSGRGRCQTGDCNGLLQCQNYGTPPNTLAEYALNQFQNLDFFDISLIDGFNVPMVFRPNSGGCTRGISCTADINGQCPGELRAPGGCNNPCTVYKTDQYCCNSGNCGPTDLSRFFKTRCPDAYSYPKDDPTSTFTCPGGTNYDFALNQYMNLDFIDISLVDGFNVPMTFRANSGTCSRSISCTANINNQCPRELRAPGGCNNPCTVYKSNQYCCNSGSCGPTPYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BPQ5</accession>
    <name>A0AAD5BPQ5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_033449</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011788">
        <property type="protein sequence ID" value="KAI7726019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BPQ5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12725">
        <property type="entry name" value="HALOACID DEHALOGENASE-LIKE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12725:SF118">
        <property type="entry name" value="PYRIDOXAL PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7726019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="70" mass="7771" checksum="F047ACF5CFB92B2C" modified="2024-05-29" version="1">MFFDDSIRNIQTAKLTGLTTVLVGSTQRKNAVDYALGSIHNIKEALPELWELVNKSKDVQKIAIETPVEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BQD8</accession>
    <name>A0AAD5BQD8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PXA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_032099</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011634">
        <property type="protein sequence ID" value="KAI7726548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BQD8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003114">
        <property type="entry name" value="Phox_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051837">
        <property type="entry name" value="SortingNexin/PXDomain-PKLike"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22999">
        <property type="entry name" value="PX SERINE/THREONINE KINASE PXK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22999:SF23">
        <property type="entry name" value="SORTING NEXIN-16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02194">
        <property type="entry name" value="PXA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51207">
        <property type="entry name" value="PXA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="16"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="39"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="PXA" evidence="4">
        <location>
            <begin position="105"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7726548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="181" mass="20923" checksum="8E3CD79B328D5488" modified="2024-05-29" version="1" fragment="single">MKAMETLHDLIEEAKIRTVWWALCIFAVSYFLAHTSKSMWMNVPIAILLVCVSRILLNEVELRWKVKKTEPLTYLSHLEKKQLSVNDSRLSTLPPPPKWKRKIDSPIVEAAMEDFINKLLQDFVVDLWYSDITPDKEAPELIRGIIMDVLAEISARVKNINLVDLLTRSVCLYNPFIVCVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BQW0</accession>
    <name>A0AAD5BQW0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_005938</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011316">
        <property type="protein sequence ID" value="KAI7727800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BQW0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02248">
        <property type="entry name" value="Peptidase_C1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000332">
        <property type="entry name" value="Cathepsin L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.160">
        <property type="entry name" value="Granulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000118">
        <property type="entry name" value="Granulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037277">
        <property type="entry name" value="Granulin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025660">
        <property type="entry name" value="Pept_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039417">
        <property type="entry name" value="Peptidase_C1A_papain-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00396">
        <property type="entry name" value="Granulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00277">
        <property type="entry name" value="GRAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57277">
        <property type="entry name" value="Granulin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00639">
        <property type="entry name" value="THIOL_PROTEASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0788">Thiol protease</keyword>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="6">
        <location>
            <begin position="1"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="Granulins" evidence="5">
        <location>
            <begin position="228"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="202"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7727800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="291" mass="31615" checksum="D99B944F38ED6A9A" modified="2024-05-29" version="1" fragment="single">SCWAFSVTGSIEGAHAIATGELISLSEQELVDCDTNDYGCDGGNMDTAYRWIIKNGGLDSEDDYPYTSSNGRDGTCDKSKLQTSVVSISSYVEVESNEDAILCAVAKQPVTIGICGSAYDFQLYTGGIYNGQCSNSIFSLDHAVLIVGYGSQDGEDYWIVKNQWGTYWGLDGYILMKRGSNVNKNGVCGMYLEPMYPIPSEPSPPSPPAPPSPPHPPPPPPTPTPSECGLFSYCAADQTCCCLFEYFNFCFEYGCCGYTNAVCCNRSSYCCPSDYPVCDVQTGYCFKVRIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BQY3</accession>
    <name>A0AAD5BQY3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_003600</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011294">
        <property type="protein sequence ID" value="KAI7727922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BQY3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7727922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="88" mass="10289" checksum="3AF43E280D2BE78E" modified="2024-05-29" version="1">MLLLSNYCLCIVICHTYYRQMLAKMQLQGKSGRFVFQINRSNQMEYEHLLFTNFNQLWPLVNLRRAISLAMLVVVLANGNGASWVEFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BR53</accession>
    <name>A0AAD5BR53_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Triosephosphate isomerase, cytosolic</fullName>
            <ecNumber evidence="7">5.3.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_032978</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-glyceraldehyde 3-phosphate = dihydroxyacetone phosphate</text>
            <dbReference type="Rhea" id="RHEA:18585"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="5.3.1.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the triosephosphate isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.1" evidence="7"/>
    <dbReference type="EMBL" id="JAMZMK010011273">
        <property type="protein sequence ID" value="KAI7728103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004807">
        <property type="term" value="F:triose-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046166">
        <property type="term" value="P:glyceraldehyde-3-phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019563">
        <property type="term" value="P:glycerol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00311">
        <property type="entry name" value="TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000025">
        <property type="entry name" value="Triosephosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00147_B">
        <property type="entry name" value="TIM_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035990">
        <property type="entry name" value="TIM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022896">
        <property type="entry name" value="TrioseP_Isoase_bac/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000652">
        <property type="entry name" value="Triosephosphate_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020861">
        <property type="entry name" value="Triosephosphate_isomerase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00419">
        <property type="entry name" value="tim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21139">
        <property type="entry name" value="TRIOSEPHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21139:SF34">
        <property type="entry name" value="TRIOSEPHOSPHATE ISOMERASE, CYTOSOLIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00121">
        <property type="entry name" value="TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51351">
        <property type="entry name" value="Triosephosphate isomerase (TIM)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00171">
        <property type="entry name" value="TIM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51440">
        <property type="entry name" value="TIM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="10" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="11" id="KW-0413">Isomerase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00011940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00039870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7728103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="252" mass="26902" checksum="BD3809317ACB7D18" modified="2024-05-29" version="1">MGRKFFVGGNWKCNGTTEDVKKIVSTLNAGELPSTDIVEVVVSPPFVFLTTVKSELRPEIQVXXXXNCWVKKGGAFTGEVSAEMLANLGVPWVILGHSERRALLNESNEFVGDKVAYALSQGLKVIACVGETLEQREAGTTMDVVAAQTKAIADKISSWDNVVLAYEPVWAIGTGKVASPAQAQEVHAGLRKWFEENISAEVSATTRIIYGGSVSGSNCKELAGQPDVDGFLVGGASLKPEFINIIKAAEAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BRR0</accession>
    <name>A0AAD5BRR0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_008469</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011241">
        <property type="protein sequence ID" value="KAI7728287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BRR0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11073">
        <property type="entry name" value="CYP76-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000007">
        <property type="entry name" value="Cytochrome P450 76C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47950">
        <property type="entry name" value="CYTOCHROME P450, FAMILY 76, SUBFAMILY C, POLYPEPTIDE 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47950:SF13">
        <property type="entry name" value="CYTOCHROME P450, FAMILY 76, SUBFAMILY G, POLYPEPTIDE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0349">Heme</keyword>
    <keyword evidence="4 5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="2 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="3 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="5">
        <location>
            <position position="449"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7728287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="507" mass="57598" checksum="E00F53EF1E9917D7" modified="2024-05-29" version="1">MEYVKLLGFVLILLVWIRWAYHRRRVHLDRLSLLPPGPTPWPVVGNIFQLGFVKVPHKSFAQLALVHGPNIMTLWLGSMCTVVISSSEAAREMFKNHDVVLAGRKVYECMKGDFGDELGSMITAQYGTNWRLLRRLSTTEFFLNSRLDATSSIRTRCIDQLVHSIEDACGTSIDVGRFFFLMAFNLIGNLMFSKDLLDPKSKKGAEFFYHTGKVMEYAGKPNVADFMPFLKGLDPQGIRKNMQNHIRQAFNIAGGFVKERMETSVADKKSTKDYLDVLLEYRGDGVEAPSVFSSTSINIIVFEMFTAGTDTTTSTLEWAMAELLRNPHTYKKLQSELRNVVSLDEKLEEKHIDNLPYLKAVIKETLRLHPPLPFLVPHKAMQSCMMLGYNIPKETQVLVNVWAIGRDPKTWENASDFKPDRFLDHNKIHVDYKGQNFEFIPFGSGRRMCPAIPLASRVLPLALGTLLHKFDWVLCDKLKATDLDMSEKMGITLRKAVPLKAIPIPKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BS72</accession>
    <name>A0AAD5BS72_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_005764</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011190">
        <property type="protein sequence ID" value="KAI7728612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BS72"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7728612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="35" mass="4089" checksum="249DDC56EB6E6F2F" modified="2024-05-29" version="1">MWWVHNMLLNTLVLLSAYVNVMAVTGTERWNLPKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BVH5</accession>
    <name>A0AAD5BVH5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_023057</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010821">
        <property type="protein sequence ID" value="KAI7730220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BVH5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043459">
        <property type="entry name" value="NFD6/NOXY2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33156">
        <property type="entry name" value="OS02G0230000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33156:SF9">
        <property type="entry name" value="PROTEIN NUCLEAR FUSION DEFECTIVE 6, CHLOROPLASTIC_MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7730220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="129" mass="14214" checksum="91D8183C7E1F81CC" modified="2024-05-29" version="1">MASRCSRLMNKASISNIKSAFRSASAPKSAPKFSLPTASPVPRFSFSRCPSELGCVQSLLPLHNAVAAARLWAQVNEGNFLNFVVVYLLDAYLFVICLARYNLEVVIDKRCLTSDSQINLLKVFDIDAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BWQ7</accession>
    <name>A0AAD5BWQ7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Rieske domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_019931</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010565">
        <property type="protein sequence ID" value="KAI7731042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BWQ7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008942">
        <property type="term" value="F:nitrite reductase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042128">
        <property type="term" value="P:nitrate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03467">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.10.10">
        <property type="entry name" value="Rieske [2Fe-2S] iron-sulphur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012748">
        <property type="entry name" value="Rieske-like_NirD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036922">
        <property type="entry name" value="Rieske_2Fe-2S_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43456">
        <property type="entry name" value="RIESKE (2FE-2S) DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43456:SF2">
        <property type="entry name" value="RIESKE (2FE-2S) DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13806">
        <property type="entry name" value="Rieske_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103511">
        <property type="entry name" value="Chlorophyll a-b binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50022">
        <property type="entry name" value="ISP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0534">Nitrate assimilation</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Rieske" evidence="8">
        <location>
            <begin position="70"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7731042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="277" mass="30168" checksum="D24E3C764388CAFF" modified="2024-05-29" version="1">MSATKLTSGLLSAPYLPHHHHRHHLPTTHQQFHNPKPTTTLALQRPTKNNIVCKATEVSSSSVSDDRLNWVPVVPLSALPKGERRVIIQDGETILLLWYKDEVFAIENRSPAEGAYSEGLINAKLTQDGCIVCPSTDSTFELRNGSIKEWFPKNPVLRALTPALRNLFVYPVKIDGENIYISLGGSAPSDASAEIVFSGMSRPGITATDVNVDEVRMVVDEVGSGGFGFTRNNEIINGKAAIIGFLLLLDFELLTGKGILKGTGFLDFLYAATNALN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BWR6</accession>
    <name>A0AAD5BWR6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Protein farnesyltransferase subunit beta</fullName>
            <shortName evidence="10">FTase-beta</shortName>
            <ecNumber evidence="2 10">2.5.1.58</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_006571</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the transfer of a farnesyl moiety from farnesyl diphosphate to a cysteine at the fourth position from the C-terminus of several proteins. The beta subunit is responsible for peptide-binding.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-cysteinyl-[protein] + (2E,6E)-farnesyl diphosphate = S-(2E,6E)-farnesyl-L-cysteinyl-[protein] + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13345"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11535"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:86019"/>
            <dbReference type="ChEBI" id="CHEBI:175763"/>
            <dbReference type="EC" id="2.5.1.58"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="10">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Heterodimer of FTA and FTB.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the protein prenyltransferase subunit beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.58" evidence="2 10"/>
    <dbReference type="EMBL" id="JAMZMK010010526">
        <property type="protein sequence ID" value="KAI7731163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BWR6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005965">
        <property type="term" value="C:protein farnesyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004660">
        <property type="term" value="F:protein farnesyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097354">
        <property type="term" value="P:prenylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02893">
        <property type="entry name" value="FTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.20:FF:000017">
        <property type="entry name" value="Protein farnesyltransferase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026872">
        <property type="entry name" value="FTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045089">
        <property type="entry name" value="PGGT1B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001330">
        <property type="entry name" value="Prenyltrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11774">
        <property type="entry name" value="GERANYLGERANYL TRANSFERASE TYPE BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11774:SF6">
        <property type="entry name" value="PROTEIN FARNESYLTRANSFERASE SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00432">
        <property type="entry name" value="Prenyltrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 10" id="KW-0637">Prenyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Prenyltransferase alpha-alpha toroid" evidence="12">
        <location>
            <begin position="72"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="14"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="326"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="337"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00050225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU365056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7731163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="460" mass="50831" checksum="34DDE416325D7F98" modified="2024-05-29" version="1" fragment="single">GYFIWRMITKRKTRKLRRTRNNMSSSPSSSATTDRKPTISQEEQWALESQVFHIYQLLYNIPPNSQAAMLELQRDSHMEYLVKGLRHLGPSFSVLDANRPWICYWILHSIALLGESVDVALQHNAIDFLSRCQDQHGGYGGGPGQASEFSTMIMPHLATTYAAVNSLITIGGQRSLESINRAKIYSFLRRMKHTSGGFSMHDGGEVDVRACYTAISVASVLNILNEELVQGVGNYIVSCQTYEGGIAGEPGSEAHGGYTFCGLATMILINEVNRLNLASLTDWLVFRQGVEGGFQGRTNKLVDGCYSFWQGGTALLIQRLHAAADSTSSEVSDDENSSDREEGAGQHSDDESSIPIRTYTETEPLFQSQALQQYILLCSQVEGGFRDKPGKSRDHYHTCYCLSGLSVAQYSRSKDSDSAPLPGSVLGPYSNLVEPVNPLYNIVLDQYNDAREFFSSAPAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-04-02" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BYM0</accession>
    <name>A0AAD5BYM0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_021405</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010626">
        <property type="protein sequence ID" value="KAI7730806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040344">
        <property type="entry name" value="At3g17950-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33544">
        <property type="entry name" value="DUF4005 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33544:SF15">
        <property type="entry name" value="OS06G0256800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7730806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="202" mass="22587" checksum="6D564F8F3604D450" modified="2024-05-29" version="1">MLDPGMELLPPLSSPTISSISSSDLDTESTGSFFHDRSTSLGTLMGVASTFPTITFRVPSQRSNTVTLGSTPTNASSVGFADRSPNRRRRRNNAVDLVSERRRKRLRRRRWWWLCSGEDLKPSSLGEFLEVERRFGEEALFGGESVMNMPVDDRNNGGTLFADGRVLPPQQTAXXXXVRPLFVLCVGFLFRLPEFVMVVLEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BZ51</accession>
    <name>A0AAD5BZ51_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_002549</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010483">
        <property type="protein sequence ID" value="KAI7731273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BZ51"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35138:SF1">
        <property type="entry name" value="MYB-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35138">
        <property type="entry name" value="OS01G0225300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7731273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="274" mass="31210" checksum="11D9E3CE6019A72E" modified="2024-05-29" version="1" fragment="single">KSFSPFFPKTYFPPFSPKPSSPPCCSNLHLPLAPTSGHILHILSELAKNDDNILNIASDLLDDNTDNVWEPPQITTTCKILSIAGSGGYGMDEIDKILYTVDKVLLGNRWLRKATGIQPKFPYVVKETGEALYVGGWKDMHCKKGKWIKDNAADAWEKHDKEYNSSLSCLGGDEMAVDQIDCLKKTLGSRRSHIRGVGRVLKSVTPNLFPIENRDEEEEHWQKQERAMEALRHDNASLRAQIKFSSYFLVCQLNLMKKMVKKTNLFKRIWFYFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BZU3</accession>
    <name>A0AAD5BZU3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">ABC transporter B family member 13-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_005585</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010402">
        <property type="protein sequence ID" value="KAI7731678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BZU3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015421">
        <property type="term" value="F:ABC-type oligopeptide transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090374">
        <property type="term" value="P:oligopeptide export from mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18577">
        <property type="entry name" value="ABC_6TM_Pgp_ABCB1_D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18578">
        <property type="entry name" value="ABC_6TM_Pgp_ABCB1_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03249">
        <property type="entry name" value="ABC_MTABC3_MDL1_MDL2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000205">
        <property type="entry name" value="ABC transporter B family member 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394:SF11">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394">
        <property type="entry name" value="ATP-DEPENDENT PERMEASE MDL1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="41"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="92"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="172"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="272"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="668"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="714"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="804"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="896"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="931"/>
            <end position="952"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="13">
        <location>
            <begin position="47"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="368"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="13">
        <location>
            <begin position="672"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="992"/>
            <end position="1200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7731678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1212" mass="131635" checksum="17CAFA34921FAB3B" modified="2024-05-29" version="1">MANQDSSEIPTAKTDLEPDSSLSEKNASFSGLFYAADKCDFLLMLCGSIGACIHGAALPVFFVLFGRMIDSLGHLSTHPHRLSSQVSKNSLYLIYLGLVVFISSWIGVACWMQTGERQTARLRFKYLQSVLRKDIKFFDTKAKDQNILFHISSDAILVQDAIGDKIGHGLRYLSQFFVGFVVGFTSVWQLTLLTLAIVPLIAFAGGAYTMIMSTLSEKSEAAYAESGKIAEENISQVRTVYSFVGENKAIDSYSKSLQNALKLARKSGFAKGVGIGFTYALLFCAWALLLWYAGILVRHHVTNGGKAFTTIINVIFSGFALGQAAPNLAAVAKGKAAAGNIVSMIKEDVDSSRKSNTGMAFSKVNGSIEFRDVCFAYPSRPNLVLDGLSFSVGAGKRIAIVGPSGSGKSTIISLIQRFYEPTSGQILLDGHDINNLQLNWLRGLMGLVSQEPALFATTIAENILYGKPDADMDHIIQVSKAANADSFIQQLPIGYQTQVGEGGTQLSGGQKQRIAIARATLRNPKILLLDEATSALDSESELIVQQSLNTVMSNRTTIIVAHRLSTIRDVDTIIVLKNGQVVEKGTHSDLVAKGGEYASLVNLQLSEPTSTKVQTNVTQHSHSHDHNFDSLATKTVNPNGENESFSISSKHAPSIWDLVKLNKPEWPYAMMGSLGAILAGMQAPLFALGITYILTSFYSGQNSKIKEDVERASFIFVAAGVVTIPIYMLQHYFYTLMGERLTTRVRLSMFSAILTNEVGWFDMDENSTGSLMSKLAADATLVRSALADRLSTIVQNISLTVTAFVISFILSWRIALVIVSTFPLLIAASITEQLFLRGFGGDYTTSYSRATSMAREAIANIRTVAAFGAEDRLSTQFATELNRPGKQARIRGHISGFGYGFSQLLAFGSYALGLWYASVLIKHRSSNFGDIIKAFMVLIITALSIAETLALAPDIVKGTQALGSVFEILNRKSAINPDQPGSLSVNRIKGDVEFRNVSFAYPTRPDIYVLNGLNLKIMAGKSMAIVGPSGSGKSTVIGLVSRFYDPVSGSVFIDDLDIKKLNLRSLRRRIGLVQQEPSLFSTTIYDNIKYGYKTEVGNNGVQLSGGQKQRVAIARAVLKDPSILLLDEATSALDTASERLVQKALDKLMEGRTTILVAHRLSTIRNADSIAVLQNGKVVECGTHDTLVGIQGSVYAHLVSLQQERNIQVDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BZZ7</accession>
    <name>A0AAD5BZZ7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">phosphopyruvate hydratase</fullName>
            <ecNumber evidence="4">4.2.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_019883</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the enolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.11" evidence="4"/>
    <dbReference type="EMBL" id="JAMZMK010010245">
        <property type="protein sequence ID" value="KAI7732405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BZZ7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000015">
        <property type="term" value="C:phosphopyruvate hydratase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004634">
        <property type="term" value="F:phosphopyruvate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03313">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.390.10:FF:000001">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.120">
        <property type="entry name" value="Enolase-like C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.10">
        <property type="entry name" value="Enolase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00318">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000941">
        <property type="entry name" value="Enolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036849">
        <property type="entry name" value="Enolase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020810">
        <property type="entry name" value="Enolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020811">
        <property type="entry name" value="Enolase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902:SF1">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00113">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03952">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00148">
        <property type="entry name" value="ENOLASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00001">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01192">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01193">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
        <property type="entry name" value="Enolase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="Enolase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enolase N-terminal" evidence="9">
        <location>
            <begin position="4"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="Enolase C-terminal TIM barrel" evidence="8">
        <location>
            <begin position="147"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7732405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="381" mass="41167" checksum="B09D69AE6CF575A5" modified="2024-05-29" version="1">MATIKAVKARQIFDSRGNPTVEVDITLSDGTLARAAVPSGASTGIYEALELRDGGSDYLGKGVSKAVANVNTIIGPALVGKDPTDQTGIDNFMVQQLDGTQNEWGWCKQKLGANAILAVSLAVCKAGASVLKTPLYKHIANLAGNKNLVLPVPAFNVINGGSHAGNKLAMQEFMILPIGASSFKEAMKMGVEVYHNLKSVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKTAIAKAGYTDKVVIGMDVAASEFYGEKDKTYDLNFKEENNDGKEKISGEQLKDLYKSFVSEYPIVSIEDPFDQDDWEHYAKMTAECGEQVQIVGDDLLVTNPTGQIKTGAPCRSERLAKYNQLLRIEEELGSEAVYAGANFRKPVEPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C1F0</accession>
    <name>A0AAD5C1F0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_009717</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the STXBP/unc-18/SEC1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010058">
        <property type="protein sequence ID" value="KAI7733250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C1F0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.830.10">
        <property type="entry name" value="Syntaxin Binding Protein 1, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043127">
        <property type="entry name" value="Sec-1-like_dom3a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001619">
        <property type="entry name" value="Sec1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036045">
        <property type="entry name" value="Sec1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11679">
        <property type="entry name" value="VESICLE PROTEIN SORTING-ASSOCIATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00995">
        <property type="entry name" value="Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56815">
        <property type="entry name" value="Sec1/munc18-like (SM) proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7733250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="138" mass="15760" checksum="81C4E80D7DF521D6" modified="2024-05-29" version="1" fragment="single">MNQGSREGGQITTRDMQKMVQALPQYNEQMDRLSLHLAIAGKINSIIRETALRDLGQLEQDLVFGDAGTKDVINFLKEQMDVTYEYKVRLLMIYAATHPEQFESEELTKLMELANLSPDDMNAVYNMRFLEAAPETIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C345</accession>
    <name>A0AAD5C345_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_009279</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009822">
        <property type="protein sequence ID" value="KAI7734130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C345"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057039">
        <property type="entry name" value="At5g52880_ARM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47744">
        <property type="entry name" value="OS05G0526300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47744:SF1">
        <property type="entry name" value="OS05G0526300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24104">
        <property type="entry name" value="At5g52880_ARM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="113"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7734130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="298" mass="33607" checksum="8518ADC539CC8689" modified="2024-05-29" version="1">MEGGGVNRYEKLGVEKSLSRSCSYPSACKELALILKLSYSKFPKLFQSFLFQDVLTAFRLLPRMQTQSAVSAANTLLQAVDSTLPKQKKTLALTEFKQAIIAHKRSSKPRLTHQDLVELPQDVLVHIFSFLDLQSLLSASQVCRSWNVASSDNHIWQSIHNMFFNPSHNFSKIDQLYGGLTEHDKSERSQESVACNSNPDWRTDFKKAYNGSLSSKKLLTSSRGLCTRCYAIVWVMDICNDTTSRPKCKYHQFIPISTSQIVKYIDGDYASSDSDSDSDSYDDFSSKLWAYPKGGFSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C3W7</accession>
    <name>A0AAD5C3W7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RanBP2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_014326</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009861">
        <property type="protein sequence ID" value="KAI7733988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C3W7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1060.10">
        <property type="entry name" value="Zinc finger, RanBP2-type"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036443">
        <property type="entry name" value="Znf_RanBP2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23111:SF108">
        <property type="entry name" value="RAN BP2_NZF ZINC FINGER-LIKE SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23111">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00641">
        <property type="entry name" value="Zn_ribbon_RanBP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00547">
        <property type="entry name" value="ZnF_RBZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90209">
        <property type="entry name" value="Ran binding protein zinc finger-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01358">
        <property type="entry name" value="ZF_RANBP2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50199">
        <property type="entry name" value="ZF_RANBP2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RanBP2-type" evidence="5">
        <location>
            <begin position="2"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="RanBP2-type" evidence="5">
        <location>
            <begin position="46"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="RanBP2-type" evidence="5">
        <location>
            <begin position="100"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7733988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="131" mass="14635" checksum="033E272002F9631B" modified="2024-05-29" version="1">MSSGDWMCAACQHLNFKKRDSCQRCHCPKFATPDEVSSYMNRTEVLAGDWYCATFNCGSHNYASRTACYRCGTVRDYSAMMAASTATCYGYDASAVPGWKTGDWICNRLGCGVHNYASRMECYKCKASRSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C3X1</accession>
    <name>A0AAD5C3X1_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_019007</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009683">
        <property type="protein sequence ID" value="KAI7734632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C3X1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041945379" evidence="1">
        <location>
            <begin position="18"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7734632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="48" mass="5393" checksum="B69C413C062EDEAA" modified="2024-05-29" version="1">MLSFLLGLKISWTLLVAFEVQAMGICCSSFLVNHFAKAFGYDVDVDEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C4M0</accession>
    <name>A0AAD5C4M0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Sister chromatid cohesion 1 protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_005327</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the rad21 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009875">
        <property type="protein sequence ID" value="KAI7733941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C4M0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008278">
        <property type="term" value="C:cohesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990414">
        <property type="term" value="P:replication-born double-strand break repair via sister chromatid exchange"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007062">
        <property type="term" value="P:sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21793">
        <property type="entry name" value="Rad21_Rec8_M_AtSYN1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.580">
        <property type="entry name" value="Structural maintenance of chromosome 1. Chain E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039781">
        <property type="entry name" value="Rad21/Rec8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006909">
        <property type="entry name" value="Rad21/Rec8_C_eu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006910">
        <property type="entry name" value="Rad21_Rec8_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023093">
        <property type="entry name" value="ScpA-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12585">
        <property type="entry name" value="SCC1 / RAD21 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12585:SF73">
        <property type="entry name" value="SISTER CHROMATID COHESION 1 PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04824">
        <property type="entry name" value="Rad21_Rec8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04825">
        <property type="entry name" value="Rad21_Rec8_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rad21/Rec8-like protein N-terminal" evidence="5">
        <location>
            <begin position="13"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="Rad21/Rec8-like protein C-terminal eukaryotic" evidence="4">
        <location>
            <begin position="543"/>
            <end position="593"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7733941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="606" mass="68592" checksum="A3E4962F89A8881A" modified="2024-05-29" version="1">MGTITKLLLMGNKKDELDPLLVAAHFPKRIKKEHVDKMNICYSVDKILVDKFPVVTYRVLAYYLIGVTRIYSKKVEYLLVDCNHSLNEMKFFSGGRKKVDVNYGGTCLPESSSQRSQLKAVDVNSSESSRRKNSYSFLESMRAQFTSLSMPDNFDLDAYDLEIVEDDSSNDHVRPHLELVLSDACESHRTVHHTFGKDHASASTSEMVTDNNPDTSVEKFRHRFVLEECLDPMVLGESDEELVLDSVPLTEQGQRQEQELEWNNDTVCTDMMSEGVQNPDKHPDEECTTTGQTVVSEMTSVDNIMLKPTPNKSHLSVTIDVTPQSKAPVVLGERKSDRVAVRTPASREHVRPPRKRKCVFDYAIVIPNEVYETWVSEASDLLRKRRTCTDVLDRKERRSFDDFMQPQIPINAGFPLELRPVISANKPVMAEEVEETVGPTPLNVLKSSGATEDEVIAPATPATHPTPLRFDEVLETSMVNPMRQASSYESGERAMFPIAGVDEDLMHRDKCPTFLVVEKLSAVTKSIGSYLHRNILQRKEKGEEEVVNLSQLLKHKTKKEAARFFYQMLVLKTGGYIDVTQEKAYDEIFVRQTQKLKEAFGGGSRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C4X3</accession>
    <name>A0AAD5C4X3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MAR-binding filament-like protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_011371</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009545">
        <property type="protein sequence ID" value="KAI7735172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C4X3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008479">
        <property type="entry name" value="DUF760"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33598:SF2">
        <property type="entry name" value="MAR-BINDING FILAMENT-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33598">
        <property type="entry name" value="OS02G0833400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05542">
        <property type="entry name" value="DUF760"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7735172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="372" mass="41236" checksum="F4714F182B1262FF" modified="2024-05-29" version="1">MAAFAASFLSTASSPPSSSSSSLSFRPNHSLPSPPTVGFTKSFIKLKTLVFASSDGIKAYEGDSRSKKSLLLKLIQEIEPLDVSIIQKDVPPTTVDAMKRTISGMLGLLPSDQFQVLIEALWKPLSKLLVSSLMTGYTLRNAEYRLCLEKNFDTDEDKQTIEDSKSPVLLNSDILINSPSGQSDQLHKSSEYTYEIPDLGQMTPEVQQYILELQSRLSSVRKELHEVKRKNAALHMQQFVGEEKNELLDYLRSLQPEKVAELSEPTSADLKETINSVVHGLLATLSPKMHSQAPLISENTSIGVANNVTGSNDAVDNTSMQFQPLVSLSRDYLARLLFWCMLLGHYLRGLEYRVELMDLLSLSQNDGSDGMM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C9Q8</accession>
    <name>A0AAD5C9Q8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Inositol polyphosphate-related phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_028758</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the inositol polyphosphate 5-phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008948">
        <property type="protein sequence ID" value="KAI7737822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C9Q8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004445">
        <property type="term" value="F:inositol-polyphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034485">
        <property type="term" value="F:phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004439">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045849">
        <property type="entry name" value="IP5P_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000300">
        <property type="entry name" value="IPPc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45666:SF66">
        <property type="entry name" value="DNASE I-LIKE SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45666">
        <property type="entry name" value="TYPE IV INOSITOL POLYPHOSPHATE 5-PHOSPHATASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22669">
        <property type="entry name" value="Exo_endo_phos2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00128">
        <property type="entry name" value="IPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Inositol polyphosphate-related phosphatase" evidence="4">
        <location>
            <begin position="38"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="35"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="74"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="75"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7737822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="333" mass="37615" checksum="9CE8A573D7B80680" modified="2024-05-29" version="1">MRLTGSDMAIPHSRFENRYSVSDRVMFGTRPGWYDPGVGSSDEENGSGDSDNGLGYDPVAYCGSFLMEERDKVVNNNNNNNNNNNNNNNNNNNNNKYCLVVSKQMVGIYLMVWVKSDLRDHVHNMKVSCVGRGLMGYLGNKGSISISMSLHQTSFCFICSHLTSGQKDGDELRRNCDVIEIMRKTRFPRDQETRYDNSPQTILDHDRIIWLGDLNYRIALSYRSAKALVEMRNWRALLEKDQASSGSYKIFGYNVGGGAYSRDGMKGAYTSLQLINIQIIQIDMQDMIRVLEKNEELLHGVTVSCGTVKGCVKCLTFEGSLDFQIIDPFTAYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C9X7</accession>
    <name>A0AAD5C9X7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_012634</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SINA (Seven in absentia) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="JAMZMK010009194">
        <property type="protein sequence ID" value="KAI7736759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C9X7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16571">
        <property type="entry name" value="RING-HC_SIAHs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000041">
        <property type="entry name" value="E3 ubiquitin-protein ligase SINAT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.210.10:FF:000004">
        <property type="entry name" value="E3 ubiquitin-protein ligase SINAT5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.210.10">
        <property type="entry name" value="Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018121">
        <property type="entry name" value="7-in-absentia-prot_TRAF-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052088">
        <property type="entry name" value="E3_ubiquitin-ligase_SINA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049548">
        <property type="entry name" value="Sina-like_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008974">
        <property type="entry name" value="TRAF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013010">
        <property type="entry name" value="Znf_SIAH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10315">
        <property type="entry name" value="E3 UBIQUITIN PROTEIN LIGASE SIAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10315:SF122">
        <property type="entry name" value="RING-TYPE E3 UBIQUITIN TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21362">
        <property type="entry name" value="Sina_RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03145">
        <property type="entry name" value="Sina_TRAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21361">
        <property type="entry name" value="Sina_ZnF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51081">
        <property type="entry name" value="ZF_SIAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="11">
        <location>
            <begin position="48"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="SIAH-type" evidence="12">
        <location>
            <begin position="101"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7736759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="297" mass="33273" checksum="7896A192820096B6" modified="2024-05-29" version="1">MAPGGNVYEDVTESRADYGVKSSLRSCGSAGGRVGMASNSGVHELLKCPVCVTVMCPPIHQCANGHTICVVCKTKVQNLCPMCREELGNIRCLALEKVAESIELPCKYHSYGCQDILPYHSRMRHEKNCKHQGYNCPYAGVECPVTGDIPFIVAHLKADHNVDMHDGSSFNHRYVKPNPHEIENATWMLTVFNCFGYQFCLHFEAFHLGTTPVYMAFLRFMGDENDANKFSYSLEVGGNGRKMKWQGIPRSIRDSHKTVRDSLDGLIITRNVALFFSGGDRQELKLKVSGRIWKEQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CBQ0</accession>
    <name>A0AAD5CBQ0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">LOB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_010458</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LOB domain-containing protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008688">
        <property type="protein sequence ID" value="KAI7738991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CBQ0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004883">
        <property type="entry name" value="LOB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03195">
        <property type="entry name" value="LOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50891">
        <property type="entry name" value="LOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LOB" evidence="2">
        <location>
            <begin position="3"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7738991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="126" mass="15025" checksum="0EC465AF37579CBE" modified="2024-05-29" version="1">MSTRCPDCVNRGTQCYPDCPFARANFTATEYLETRKYNNIDWVRDKLQELSKHEVEYYKRSILYENKCRREDPIGGCLKVNDHVVKAIETLYGQMVKIGYNYLKNKKKEDDDEERNYLIRAELKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CC67</accession>
    <name>A0AAD5CC67_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_013261</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008958">
        <property type="protein sequence ID" value="KAI7737766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CC67"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31150">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31150:SF23">
        <property type="entry name" value="MANDELONITRILE LYASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="3">
        <location>
            <begin position="209"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="114"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="337"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="27"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="37"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="130"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="342"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="356"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7737766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="368" mass="40532" checksum="EE9A2526BC9C2F00" modified="2024-05-29" version="1">MGPHEPYWRTNTSFSPPPPRWDFRFQNEGQSFGSQEGSRLYGSSTSSNSRGSRNWFRGNYLPNHRHSTSDGFGAFFSSPSELSPVQQWTPPTVQEISLDEYANSSKRVLAPLSFSPTSEGLSAPHDGRGSISSNSDSSDYDPIVKSQSYQHNFTTRGCFMSKPVHPLAFPNNPRFQDPDDNNLHSEFEFSDVSEPVEPEYLNRSNAHKCNLCERFLSQRSPWSSCQIIKSGDMPVIGVLSCRHVFHAECLDQTTPKTQNGDPPCPVCAKSETKYSPDRRVSSKLKSGFPRVRPFREDGPSRPWGCVQAGNCVEGALSAQPSHNAMLLLNRNRVKKSLSLKKGGTGSSQSGGGDGVGSSKSASSSRKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CD96</accession>
    <name>A0AAD5CD96_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_027539</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008826">
        <property type="protein sequence ID" value="KAI7738336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CD96"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010903">
        <property type="entry name" value="DUF1517"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053023">
        <property type="entry name" value="FLAP_modulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33975">
        <property type="entry name" value="MYELIN-ASSOCIATED OLIGODENDROCYTE BASIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33975:SF2">
        <property type="entry name" value="MYELIN-ASSOCIATED OLIGODENDROCYTE BASIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07466">
        <property type="entry name" value="DUF1517"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037221">
        <property type="entry name" value="DUF1517"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7738336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="304" mass="33954" checksum="A05BFB473C9E711A" modified="2024-05-29" version="1">MNHKPQVEKGSGLTKVATETGINGVFRRPMENVMKALQVSVIAGVLSGLLLLYDHNEGGLALAASCDETSGQASCSSEVYSTQTSSPACMEPQKGHEPELQHLIFPVVLVSCFIIKWFDDKMHSEEKSVLKLQFLKVGLLGTSLQSDLKRIAATSTTKGLSYVLQEATLALLRHRDYCIYGYSSVDLKTKPGEGQKRFNHLSIKERAKINKETLVNVNDTRNISTSQSSNNAYIVVTILVYAWYHRWKLPPVTSSEQLKEALQNLASIPSDYIINVEVLWNPQHENDFLTEEELLKDYPLMHSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CER0</accession>
    <name>A0AAD5CER0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">H(+)-transporting two-sector ATPase</fullName>
            <ecNumber evidence="3">7.1.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">ATP synthase F1 sector subunit beta</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">F-ATPase subunit beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">M8C21_018139</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">AA19_3_7</strain>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Produces ATP from ADP in the presence of a proton gradient across the membrane. The catalytic sites are hosted primarily by the beta subunits.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>ATP + H2O + 4 H(+)(in) = ADP + phosphate + 5 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:57720"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.1.2.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.1.2.2" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010008414">
        <property type="protein sequence ID" value="KAI7740536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CER0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042776">
        <property type="term" value="P:proton motive force-driven mitochondrial ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050053">
        <property type="entry name" value="ATPase_alpha/beta_chains"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15184">
        <property type="entry name" value="ATP SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15184:SF71">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT BETA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0139">CF(1)</keyword>
    <keyword evidence="6" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-1278">Translocase</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ATPase F1/V1/A1 complex alpha/beta subunit nucleotide-binding" evidence="17">
        <location>
            <begin position="3"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00037290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00042624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00042742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAI7740536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="76" mass="8288" checksum="DE9AED7B4A594936" modified="2024-05-29" version="1">MELINNIAKAHGGVSVFDGVGERTREGNDLYMEMKESGVINEQNIAESKVALVYGQMNEPSGARMRVGLTTLTMVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CEV7</accession>
    <name>A0AAD5CEV7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_027115</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008657">
        <property type="protein sequence ID" value="KAI7739221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CEV7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.110.10:FF:000002">
        <property type="entry name" value="Phosphatidylinositol 4-phosphate 5-kinase 8"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.110.10">
        <property type="entry name" value="Histone H3 K4-specific methyltransferase SET7/9 N-terminal domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084:SF179">
        <property type="entry name" value="OS10G0565000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23084">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82185">
        <property type="entry name" value="Histone H3 K4-specific methyltransferase SET7/9 N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="177"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="200"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="9"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="36"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="75"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7739221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="547" mass="61013" checksum="FFDC066C74AB696C" modified="2024-05-29" version="1">MHQKKSEPQIGTDSTGISSDFTPNPIISSSIPVFRSSPLSPASTSSPSPSSSFLLLPPPHNNNNNNNNIHVSPLLSPPSIPNPNPNINNNNNNVSHQHNILIDDLDIDIDPTPKSTPHKRPQFSQFTQLASNSGSDLFTKTPVLSNRFSSKIRSSNRFCNVKAAGFRVLNRIRRLRTHIRLILLLSLPFFYFLVSHPSQSFVLDFLSAFAFSTALLFSLNLAVPRLPSIRLFLSRTLAIKPNSLRPHLHLPVFWSIGGRNNKRGGSGCWVQAYNNGDVYEGEFHKGKCSGCGVYYYYMSGRYEGDWVDGKYDGYGVETWARGSRYRGQYRQGLRHGFGVYTFYTGDVYAGEWSNGQSHGCGVHTCEDGSRYVGEFKWGVKHGLGHYHFRNGDTYAGEYFADKMHGFGVYSFANGHRYEGSWHEGKRQGLGMYTFRNGETQSGHWQNGVLDVPSSQTTMHPVSPVGVYHSKVLNAIQEARRAAEKAYDVAKVDERVNRAVAAANRAANAARVAAIKAVQKQMHHRHNSDGLPIPMVQNFHRLSTQLPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CFJ2</accession>
    <name>A0AAD5CFJ2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_012732</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="6">Binds 1 Fe(2+) ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the alkB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008445">
        <property type="protein sequence ID" value="KAI7740385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CFJ2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035516">
        <property type="term" value="F:broad specificity oxidative DNA demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035515">
        <property type="term" value="F:oxidative RNA demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035513">
        <property type="term" value="P:oxidative RNA demethylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.590">
        <property type="entry name" value="Alpha-ketoglutarate-dependent dioxygenase AlkB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004574">
        <property type="entry name" value="Alkb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027450">
        <property type="entry name" value="AlkB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037151">
        <property type="entry name" value="AlkB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16557:SF10">
        <property type="entry name" value="2-OXOGLUTARATE-DEPENDENT DIOXYGENASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16557">
        <property type="entry name" value="ALKYLATED DNA REPAIR PROTEIN ALKB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13532">
        <property type="entry name" value="2OG-FeII_Oxy_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="8">
        <location>
            <begin position="269"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="26"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="41"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="287"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="289"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="347"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR604574-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7740385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="379" mass="42026" checksum="BE54ED41713C6B0D" modified="2024-05-29" version="1">MERTPSKLLKDDSLSIYEKTLLKLQQGSQLDLSSVPEESTKTSMSPSVSTVSSNSEPSLTMNAADCASPVSFPGSGSSQSMNISKEEPRGNLSVIAMFSRYKASRSDKIISSDDSAMEIEDYGFSANTSPSHDPELQEGCIIDPPSPVGRKTTNPVCERTPSKLSTYTILEPGMIHLQNYISLKDQVDIVNICQKWAGEFYNPRDPQNGAELNLMMMCFGRNWDPNTGYKERYRSDGSEPPPIPYELISLAEAAVRDAQAILNELPSMHPDICSVNFYPAYGLLDLHQDHDESSNSLRRGLPVVSISIGECAEFVYGHTRAKKKLKKIYLMSGDVLIFGGKSRLIYHGVTQIAQYTSPLKLIEETALRRGRLSLTFRQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-10-08" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CGG5</accession>
    <name>A0AAD5CGG5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_000177</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008186">
        <property type="protein sequence ID" value="KAI7741481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039271">
        <property type="entry name" value="Kiwellin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33191:SF77">
        <property type="entry name" value="RIPENING-RELATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33191">
        <property type="entry name" value="RIPENING-RELATED PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24300">
        <property type="entry name" value="KWL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042146754" evidence="5">
        <location>
            <begin position="23"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="74"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7741481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="107" mass="11445" checksum="ECB60F02447D4F30" modified="2024-05-29" version="1">MKRFHFIMLFVLLAAISVQINAQTCKPSGGIRGRKPPPGQCNKENDSDCCVQGKFXXXXXPTVTSATKATLTINSFQKGGDGGGPSECDNKYHSDDTPVVALSTGWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CH35</accession>
    <name>A0AAD5CH35_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_001198</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="JAMZMK010008349">
        <property type="protein sequence ID" value="KAI7740765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CH35"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13983">
        <property type="entry name" value="STKc_WNK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000075">
        <property type="entry name" value="Probable serine/threonine-protein kinase WNK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000046">
        <property type="entry name" value="probable serine/threonine-protein kinase WNK9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050588">
        <property type="entry name" value="WNK_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13902">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE WNK WITH NO LYSINE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000615">
        <property type="entry name" value="TyrPK_CSF1-R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="27"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="155"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000615-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000615-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7740765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="294" mass="33797" checksum="D206F0885AEFDEE2" modified="2024-05-29" version="1">MMPAMEPDMVNRDKEPFVEVDPTGRYGRYNELLGSGAVKKVYRAFDQEEGIEVAWNQVKLRNFSDDQCMIERLYSEVKLLTTLKNKNIITLFWHWRDGEKNTLNFITEVCTSGNLRDYRNKHRHVSMQAVKKWCRQILRGLDYLHTHDPCIIHRDLNCSNILINGNIGQVKIGDLGLAAIVGKSHCAHSILGTPEFMAPELYEEDYTELVDIYSFGMCLLELVTLELPYSECNNVARIYKKVTSGVRPDAMKKVKDPEVKAFIEKCLAQPRDRPSAADLLKDPFFDNICDDDEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CHB8</accession>
    <name>A0AAD5CHB8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">A to I editase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_026738</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008105">
        <property type="protein sequence ID" value="KAI7741917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CHB8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003726">
        <property type="term" value="F:double-stranded RNA adenosine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008251">
        <property type="term" value="F:tRNA-specific adenosine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006382">
        <property type="term" value="P:adenosine to inosine editing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002466">
        <property type="entry name" value="A_deamin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10910:SF62">
        <property type="entry name" value="AT07585P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10910">
        <property type="entry name" value="EUKARYOTE SPECIFIC DSRNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02137">
        <property type="entry name" value="A_deamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50141">
        <property type="entry name" value="A_DEAMIN_EDITASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="A to I editase" evidence="1">
        <location>
            <begin position="33"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7741917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="179" mass="20330" checksum="6B5EA8156E3C1F31" modified="2024-05-29" version="1">MVLVDQDDDEDDEIFKGDCSHVTGTVQRKPGRGDATLSVCCSDKISRWNVVGVQGALLSHFLQPVYISSITMGQSRNCSDNEVEEQLTRALSDRILPLSKKLTSPFKVNKVKKNLVHRYVMDIREAEVFSERLKRKLNALEAQMCIWSRRLHRSLVLVLVTDYLQVTPVKELSKVESLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CLF4</accession>
    <name>A0AAD5CLF4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Heat stress transcription factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">M8C21_001745</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">AA19_3_7</strain>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the HSF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007617">
        <property type="protein sequence ID" value="KAI7743978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CLF4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000057">
        <property type="entry name" value="Heat shock transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000232">
        <property type="entry name" value="HSF_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015:SF428">
        <property type="entry name" value="HEAT SHOCK FACTOR PROTEIN HSF8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015">
        <property type="entry name" value="HEAT SHOCK TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00447">
        <property type="entry name" value="HSF_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00056">
        <property type="entry name" value="HSFDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00415">
        <property type="entry name" value="HSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00434">
        <property type="entry name" value="HSF_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0346">Stress response</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HSF-type DNA-binding" evidence="14">
        <location>
            <begin position="75"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="123"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="452"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="153"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="131"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="458"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00055747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00081483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAI7743978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="479" mass="53528" checksum="AAF6C189006AF964" modified="2024-05-29" version="1">MEPPTHTNPAVSAATDGGVPPPAPLPMSSVNAPPPFLVKTYDMVDDPSTDKVVSWTSTNNSFVVWDPPEFARDLLPKYFKHNNFSSFVRQLNTYGFRKVDPDRWEFANEGFLRGQKHLLKSIARRKSASGHTQPPQQPHQQTSSVGACVEVGKFGLEEEVERLKRDKNILMQELVRLRQQQQTTDNQMQSMVQRLQGMEQRQQQMMSFLAKAVNSPGFLAQFVQQQNESTRLITEGNKKRRYKEDSVKSNEHSSDGQIVKYQQPAMNDAAQAMLKQLMKLNNSSPRLNTFSSGVTLHEVTPPASGQSYLPAVTDVLSAPLEANSEVIAADHFPDTSPIVDGQELPDVSDISQLQELVSDVSVDSYMHPEAQNQALLDSNLVGEKLELEIGSFSPDADVEWDSNLLTEMEKYLTAYDPSWEQFLEQSPEANTNDETDSGPVDDDVIMGSEIKSSETECSRSQMQQLTKQMGLLSSDTRKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CLI8</accession>
    <name>A0AAD5CLI8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">calcium/calmodulin-dependent protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">M8C21_004917</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="18">AA19_3_7</strain>
            <tissue evidence="18">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.17"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.17"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.17" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010007607">
        <property type="protein sequence ID" value="KAI7744038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CLI8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004683">
        <property type="term" value="F:calcium/calmodulin-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000610">
        <property type="entry name" value="Calcium and calcium/calmodulin-dependent serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000249">
        <property type="entry name" value="calcium and calcium/calmodulin-dependent serine/threonine-protein kinase DMI-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050205">
        <property type="entry name" value="CDPK_Ser/Thr_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24349">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13202">
        <property type="entry name" value="EF-hand_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00450">
        <property type="entry name" value="RECOVERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0106">Calcium</keyword>
    <keyword evidence="12" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="12"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="17">
        <location>
            <begin position="390"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="17">
        <location>
            <begin position="426"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="17">
        <location>
            <begin position="468"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="KAI7744038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="514" mass="57138" checksum="1704C3F6BD4C5F7F" modified="2024-05-29" version="1">MDHGAKRLSDEYEVTTILGRGGFSIVRKGIKKSKSDFEDHEKEVAIKTLKRVGGGGGPSSGMTRFPSRNQVLISDALLANEIMVMKRVVEEVSPHSNVIQLHDVCEDATGVHLVLELCSGGELFDRIVSQERYSEAEAAKVVQQIASGLAALHRANVIHRDLKPENCLFLSKSKDSPLKIMDFGLSSVEEFTDPVVGLFGSIDYVSPEALSQQKITSKSDMWALGVILYILLSGYPPFSAQSHLQKQQMIMNGNFSFYDKTWKKISQSAKDLIKSLLNVDPQKRPTADQILKHPWVIGDSATERLIDPEIVSRLQSFNARRKFRAAAIASMYTCTVFLRTKKLKNLVGSYDLNQEELQNLRNHFKTLCADGENATLSEFQEVLKAMKMTSLIPLAGRIFDLFDNNRDGTVDMREILCGFSSLKNLKGDDALRLCFQMYDADGSGCISKEEVASMLRALPDECLSIDITEPGKLDEIFDHMDANSDGKVTFDEFKAAMQKDSSLQDVVLSSICPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CLP8</accession>
    <name>A0AAD5CLP8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">monodehydroascorbate reductase (NADH)</fullName>
            <ecNumber evidence="8">1.6.5.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_013584</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FAD-dependent oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.6.5.4" evidence="8"/>
    <dbReference type="EMBL" id="JAMZMK010007813">
        <property type="protein sequence ID" value="KAI7743090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CLP8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016656">
        <property type="term" value="F:monodehydroascorbate reductase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050446">
        <property type="entry name" value="FAD-oxidoreductase/Apoptosis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048618">
        <property type="entry name" value="MDHAR3-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43557">
        <property type="entry name" value="APOPTOSIS-INDUCING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43557:SF6">
        <property type="entry name" value="MONODEHYDROASCORBATE REDUCTASE, CHLOROPLASTIC_MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21791">
        <property type="entry name" value="MDHAR3-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="9">
        <location>
            <begin position="55"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="domain" description="Monodehydroascorbate reductase 3-like C-terminal" evidence="10">
        <location>
            <begin position="387"/>
            <end position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF21791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7743090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="488" mass="52738" checksum="0812FE16CF0EFDF3" modified="2024-05-29" version="1">MATISTSVLIKSGVSLWCPRLDCQHSSVIGSRSFQRRNFVVSASSSSFANDNREFVIVGGGNAAGYAARTFVEHGMADGKLCIVSKEAYAPYERPALTKGYLFPLDKKPARLPGFHTCVGSGGERQTPEWYTEKGIEMIYEDPVTAIDAEKQTLTTNSGKLLKYGSLIIATGCTASRFPEKIGGNLHGVHYIRDVADADSLVSSLAKAQKVVVVGGGYIGMEVAAAAVGWNLDTTVIFPETNLLPRLFTPSLAQKYEELYQENGVKFIKGASIKSLEAGPDGNVTAVKLENGSTIEADLVVIGIGAKPAVSPFDTAVNLNKTVGGIEVDGLFRSSLPGIFAVGDVAAFPLKMYNKISRVEHVDHARRSAQHCVKALLTAQTHTYDYLPYFYSRVFEYEGSSRKVWWQFFGDNVGEAIQIGNFDPKIATFWLDSGKLKGVLLESGSPEEFQLLPKLAKSQPSIDKAKLQSAASVEEALEIARASILETA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CM17</accession>
    <name>A0AAD5CM17_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_021021</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007588">
        <property type="protein sequence ID" value="KAI7744074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CM17"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="135"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="135"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="175"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7744074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="184" mass="18753" checksum="B6EFA6027BC6B9D0" modified="2024-05-29" version="1">MEADNDKVKVATPVFPPTPVTVVDNTTPTPVTFVDNTAPTPVTVVDNTAPTPVTFVDNTTPAPVIYLNSYDPAASTSSQPLPQKGAVKLSIKSDKTAGKPLQIAFGISVESASFIGSDIDSVDLLISFDFSSLKNQTDSTTVDSNPGAESNNPLIVPIGGGKINPGDAAADGGRISSGGGGQTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CM91</accession>
    <name>A0AAD5CM91_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Na+/H+ antiporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_000990</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007562">
        <property type="protein sequence ID" value="KAI7744232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CM91"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015386">
        <property type="term" value="F:potassium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015385">
        <property type="term" value="F:sodium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051453">
        <property type="term" value="P:regulation of intracellular pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098719">
        <property type="term" value="P:sodium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018422">
        <property type="entry name" value="Cation/H_exchanger_CPA1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10110:SF172">
        <property type="entry name" value="CATION_H+ EXCHANGER, CATION_H+ EXCHANGER, CPA1 FAMILY, NA+_H+ EXCHANGER NHX -TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10110">
        <property type="entry name" value="SODIUM/HYDROGEN EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0630">Potassium</keyword>
    <keyword evidence="2" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7744232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="153" mass="17094" checksum="FAF99F1F0374E2D0" modified="2024-05-29" version="1" fragment="single">ITVWWAGLMRGAVSVALAYKKFTVSDEQHMEPANALLITSTIIIVLFSTVVFGLLTKPIINMLTKSSNVDQTQPSSPKSIMMPLIGHRLDLEAETVDDETSLETTTNLKVMFSNPTNTIHYYWRKFDDAFMRPVFGGRGFISYVTGTRDVALT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CNU4</accession>
    <name>A0AAD5CNU4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ResB-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_013551</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007370">
        <property type="protein sequence ID" value="KAI7745079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CNU4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017004">
        <property type="term" value="P:cytochrome complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01392">
        <property type="entry name" value="CytC_Ccs1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023494">
        <property type="entry name" value="Cyt_c_bgen_Ccs1/CcsB/ResB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007816">
        <property type="entry name" value="ResB-like_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31566">
        <property type="entry name" value="CYTOCHROME C BIOGENESIS PROTEIN CCS1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31566:SF0">
        <property type="entry name" value="CYTOCHROME C BIOGENESIS PROTEIN CCS1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05140">
        <property type="entry name" value="ResB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0201">Cytochrome c-type biogenesis</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="99"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="164"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="248"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="ResB-like" evidence="7">
        <location>
            <begin position="105"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="ResB-like" evidence="7">
        <location>
            <begin position="434"/>
            <end position="517"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7745079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="536" mass="58625" checksum="019397F9C5A351D1" modified="2024-05-29" version="1">METLLTLNPLKPQPHFFKYPNSRTYTPVTNNNNCRGLFCPVSCKLSSQPSKDTTKNKVVVPGAAPSLSEDNEPPAVVESRSKNTAGVVVRRLSKRVLSFLANLPLALGEMFTIAGLMALGTAIDQGEKPEYYFQKYPEDNPILGFFTWRWILTLGFDHMFSSPVFLGTLVLLAASLMACTYTTQIPMVKVARRWSFLHSANTIRKLEFSDSLPKASIQDLGVILSGSGYEVFLKGPSLYAFKGLAGRFAPIGVHIALLLIMGGGTYSAIGSFKGSVTVPQGLNFVMGDVLGPNGFLSTPTEAFNTEVHVNRFYMDYYDSGEVSQFHTDLSLFDIDGKEVMRKTISVNDPLRYEGVTIYQTDWSISALQILKDDEGPYNLAVAPLTVNGDKKLYGSFLPVGNADSTNVKGISMLLRDLQSVVLYDEEGKFAGVRRPNSKLPIEINGTKIEIVDAIGSSGLDMKTDPGVPIVYAGFGALMLTTCISYLSHTQIWALQDGTTVVVGGKTNRAKIEFPDEMNRVLDRVPEINEPSDVICG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CPF8</accession>
    <name>A0AAD5CPF8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cytochrome b-c1 complex subunit 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_027954</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the UQCRH/QCR6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007278">
        <property type="protein sequence ID" value="KAI7745342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CPF8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006122">
        <property type="term" value="P:mitochondrial electron transport, ubiquinol to cytochrome c"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.20:FF:000001">
        <property type="entry name" value="Cytochrome b-c1 complex subunit 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.20">
        <property type="entry name" value="Ubiquinol-cytochrome C reductase hinge domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003422">
        <property type="entry name" value="Cyt_b-c1_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023184">
        <property type="entry name" value="Ubol_cytC_Rdtase_hinge_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036811">
        <property type="entry name" value="Ubol_cytC_Rdtase_hinge_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15336:SF0">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT 6, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15336">
        <property type="entry name" value="UBIQUINOL-CYTOCHROME C REDUCTASE COMPLEX 7.8 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02320">
        <property type="entry name" value="UCR_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000019">
        <property type="entry name" value="Bc1_11K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81531">
        <property type="entry name" value="Non-heme 11 kDa protein of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 10" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 10" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Ubiquinol-cytochrome C reductase hinge" evidence="12">
        <location>
            <begin position="8"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="17"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="31"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR000019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000019-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7745342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="71" mass="8413" checksum="1698762F5836B357" modified="2024-05-29" version="1">MSDEEPVDQKKYFEDLCKPKCVRAWLDYQGCVKRVQADETGHKHCTGQYFDYWQCVDKCVSLFTCTFLFIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CPG6</accession>
    <name>A0AAD5CPG6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_013817</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007544">
        <property type="protein sequence ID" value="KAI7744364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CPG6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000060">
        <property type="entry name" value="MYB transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050560">
        <property type="entry name" value="MYB_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614:SF25">
        <property type="entry name" value="MYB PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614">
        <property type="entry name" value="MYB PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="56"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="56"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="108"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="112"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="45"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7744364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="348" mass="37976" checksum="B33F86C01D8EB3D8" modified="2024-05-29" version="1">MNDEAADTVMLEQTNKTTSDSSPKEAGEVLNELMVVDDGGGGEDNNNNNNNNNNNNNNKVKGSWSPKEDALLSDLVSKFGPKNWSLIARGIPGRSGKSCRLRWCNQLDPAVQRKPFSNAEDRTIIEAHKIHGNKWALIAKLLPGRTDNAIKNHWNSTLRRRSTHPNVYLIPSNINYNNFDTTQASCEDTFSTENNISPTKLTETEHVTTHYISADPVLTHETVPRPVAHFGAFSAFTPANGIQNEPKMVVPTQGPLIQACKPESVGGKFLKGYSIESAVPSRCGHGCCSGPQTSLLGPEFVEYDEVPALSNMELATIAADLNTIAWIKSGLERPQTQSQISLPVEGLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CRW1</accession>
    <name>A0AAD5CRW1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Exocyst complex component SEC5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_031836</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the exocyst complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the SEC5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007044">
        <property type="protein sequence ID" value="KAI7746115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CRW1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029175">
        <property type="entry name" value="EXOC2/Sec5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039481">
        <property type="entry name" value="EXOC2/Sec5_N_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13043:SF1">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13043">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT SEC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15469">
        <property type="entry name" value="Sec5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst complex component EXOC2/Sec5 N-terminal" evidence="6">
        <location>
            <begin position="11"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="469"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="551"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="488"/>
            <end position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU365069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF15469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7746115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="579" mass="64258" checksum="2C7E471827BFF724" modified="2024-05-29" version="1">MFPNDSLISNSQPRDSGSEELDALRGRYIRRLTAVIVHHIPAFWKVALSVSSGKFAKVGDEKYSSHSLDEVSGMIQNTISAYESKVHNTFLDLGESNILRPYMSDAITDISKACEAFEAKEAAPTVAVAALRTLQYEITKTYIQRLSLWMRSSTEEISKNELWVPVSVLERNKSSYTISHLPLAFRSVMVTAMDQIDMMMQSLGSEATKSEDAFVLFQETQESVRVSFLNCLLDFAGHLEQIGSELGQNISNKENSSYQNGYPEENSFEPLPGSITNPHQQLLMVLSNLGFCKDDLSREMHHKYKHIWMQPRGKDEADNDVDDLVRSFSGLEEKVLAQYTMAKANHIRTAAVNYLLDAGVQWGGVPAVKGVRDASVELLHTLVAVHAEVSSGCKPLLDKILGILVEGLMDTLLSLFHEHKDTDIRILDANGFCQLMLELEYFETILNQYFTPEVSESLRSLQGAILEKATESSSEAGDTPKHRRRATRGSDDLMDDKQGTVSPDELIALAQQCSNELLQSELKRTQINTACFVDSLPLDPGPTAVKAVAGYGSFRDSPGRSFGAKQATGSPSFSRPKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CSH3</accession>
    <name>A0AAD5CSH3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Tubulin beta chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">M8C21_004638</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">AA19_3_7</strain>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11 12">Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="12">Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the tubulin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006972">
        <property type="protein sequence ID" value="KAI7746460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CSH3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02187">
        <property type="entry name" value="beta_tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.600:FF:000002">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.20:FF:000002">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000005">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.600">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013838">
        <property type="entry name" value="Beta-tubulin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002453">
        <property type="entry name" value="Beta_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023123">
        <property type="entry name" value="Tubulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01163">
        <property type="entry name" value="BETATUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00228">
        <property type="entry name" value="TUBULIN_B_AUTOREG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9 12" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 12" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="14">
        <location>
            <begin position="47"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="15">
        <location>
            <begin position="246"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="419"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="429"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAI7746460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="445" mass="49996" checksum="BBAD936FB876DE1D" modified="2024-05-29" version="1">MREILHIQGGQCGNQIGAKFWEVVCAEHGIDVTGKYTGDSELQLERINVYYNEASGGRFVPRAVLMDLEPGTMDSIRSGAYGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVLDVVRKEAENCDCLQGFQVCHSLGGGTGSGMGTLLISKIREEYPDRMMMTFSVFPSPKVSDTVVEPYNATLSVHQLVENADECMVLDNEALYDICFRTLKLTTPSFGDLNHLISATMSGVTCCLRFPGQLNSDLRKLAVNLIPFPRLHFFMVGFAPLTSRGSQQYRALTVPELTQQMWDAKNMMCAADPRHGRYLTASAIYRGKMSTKEVDEQMLNVQNKNSSYFVEWIPNNVKSTVCDIPPTGLKMASTFIGNSTSIQEMFRRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATADEEGEYEEEEEYDEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CSP0</accession>
    <name>A0AAD5CSP0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_019119</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006718">
        <property type="protein sequence ID" value="KAI7747603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CSP0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="10"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="52"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7747603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="280" mass="31744" checksum="827DE89AC7C56712" modified="2024-05-29" version="1">MGYYQASTDNLQKLARDFFSAMDHDGDGKIDLNEFMEFMKEEGYSQMTNPFIFNQLDLDGNGTLDFVEVMTVYYIIKSGRPFCDFCKKFIASTYLTCVACLEDPNSQPFYLCLDCYIMQKCDHTHNNLSRFVDNYSLLEAMTKLKFGNELRSSDSRSRPLETDSVLVTDKPCSGDNNTWNPSLAMVQVPPRPRPVHDQQWWVQGATPEAPPTSVAPSVHNHTWIQNNYSYNHTPSHHIQQPTNFNAIITNRQNWKIALGALNVAVQIIGTSTAGSLCTIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CTI0</accession>
    <name>A0AAD5CTI0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">M8C21_022859</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">AA19_3_7</strain>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010006711">
        <property type="protein sequence ID" value="KAI7747662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CTI0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12195">
        <property type="entry name" value="CIPK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14663">
        <property type="entry name" value="STKc_SnRK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000279">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000096">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.80:FF:000005">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.80">
        <property type="entry name" value="Kinase associated domain 1, KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018451">
        <property type="entry name" value="NAF/FISL_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004041">
        <property type="entry name" value="NAF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895">
        <property type="entry name" value="CALCIUM/CALMODULIN-DEPENDENT PROTEIN KINASE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895:SF163">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03822">
        <property type="entry name" value="NAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50816">
        <property type="entry name" value="NAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="77"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="NAF" evidence="15">
        <location>
            <begin position="363"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="335"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="496"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="345"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="497"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAI7747662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="519" mass="58537" checksum="4844434F105F6D82" modified="2024-05-29" version="1">MLKDDPKPVSELHCDSASLHLAVFVLDETGCILSSSSDPCAKTQGSTTDASLMHELCEDDTNMTSLDSKPSVLMGRYELGRLLGQGTFAKVYYGRNTRTGQSVAIKVIDKEKVLRVGLTNQIKREISIMRLVKHPNILELYEVMATKTKIYFALEYAKGGELFDKVSKGRLKEQMARKYFQQLISAVDFCHSRGVYHRDLKPENLLLDENDNLKVSDFGLSALAECKHQDGLLHTTCGTPAYVAPEVINRKGYEGDKADIWSCGVILYVLLTGYLPFHDSNLMEMYRKIGKAEFKCPHWFPPEVKRLLTRMLDPNPGYRITIAKIKENPWFKKGTIARKPRNETENTGSDRASGSSEEKQEVIRPPNLNAFDIIALSPGFDLTALFQERGQKKEARFTSWKPASVIISKLEEVAKLLKMKISKREAGLLKLEATKDGRKGILAIDAEIFELTHSFHMVEVKKSNGDTLEYQKVLNEGLRPGLQDIVWTWQPEEEPPEQIVQPEQLGPLPPQQEHSTTII</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CU48</accession>
    <name>A0AAD5CU48_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NmrA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_005740</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006942">
        <property type="protein sequence ID" value="KAI7746586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CU48"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008030">
        <property type="entry name" value="NmrA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050608">
        <property type="entry name" value="NmrA-type/Isoflavone_red_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43349:SF70">
        <property type="entry name" value="NMRA-LIKE DOMAIN, NAD(P)-BINDING DOMAIN SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43349">
        <property type="entry name" value="PINORESINOL REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05368">
        <property type="entry name" value="NmrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NmrA-like" evidence="1">
        <location>
            <begin position="3"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7746586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="192" mass="20648" checksum="8829C7AE0664B7D1" modified="2024-05-29" version="1">MAQKTKILIIGGTGYIGKFVVEASVKEGHPTFVLVRENTTTKHPEKANLIHNFKTLGATILYGDIHDHESLVKAIKHVDVVISTVGGGLVADQVKLIAAIKEAGNVKRFLPSEFGTDVDHVNAVEPAKTTFAGKARIRRAVEAADIPHTYISCNGFAGYFLPTVGQMDTHTAPREKITILGDGNPKGRIINA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CUF7</accession>
    <name>A0AAD5CUF7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Phosphotransferase</fullName>
            <ecNumber evidence="11">2.7.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_011052</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>D-fructose + ATP = D-fructose 6-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16125"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37721"/>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:16126"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a D-hexose + ATP = a D-hexose 6-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22740"/>
            <dbReference type="ChEBI" id="CHEBI:4194"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:229467"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:22741"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate metabolism; hexose metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the hexokinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.-" evidence="11"/>
    <dbReference type="EMBL" id="JAMZMK010006899">
        <property type="protein sequence ID" value="KAI7746751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CUF7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005536">
        <property type="term" value="F:D-glucose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008865">
        <property type="term" value="F:fructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004340">
        <property type="term" value="F:glucokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001678">
        <property type="term" value="P:intracellular glucose homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd24020">
        <property type="entry name" value="ASKHA_NBD_HK_plant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.367.20:FF:000003">
        <property type="entry name" value="Phosphotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.367.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001312">
        <property type="entry name" value="Hexokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022673">
        <property type="entry name" value="Hexokinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022672">
        <property type="entry name" value="Hexokinase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19443">
        <property type="entry name" value="HEXOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19443:SF16">
        <property type="entry name" value="HEXOKINASE TYPE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00349">
        <property type="entry name" value="Hexokinase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03727">
        <property type="entry name" value="Hexokinase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00475">
        <property type="entry name" value="HEXOKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51748">
        <property type="entry name" value="HEXOKINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 11" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="6 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Hexokinase N-terminal" evidence="12">
        <location>
            <begin position="41"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="Hexokinase C-terminal" evidence="13">
        <location>
            <begin position="220"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF03727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7746751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="473" mass="51320" checksum="BD1794A6DBA899E4" modified="2024-05-29" version="1">MGKVAVAAAVVGVAAVSAAVVLVVRHRMQSSGKWAKAMDIVKEFDEKCATPIAKLRQVADAMTVEMHAGLASDGGSKLKMLISYVDNLPTGDETGVFYALDLGGTNFRVLRVKLGEVDNVKKEFEEVSIPPELMIGKSDDLFDFIAGELAKFVATEGEDMQIPAGTQRELGFTFSFPVKQSSIAGGTLVRWTKGFNIEDAVNDTVGTLAGGRYTDPNVIAAVILGTGTNAAYVERANAIPKWQGLLPKSGEMVINMEWGNFRSSHLPLSEYDENLDSDSLNPGEQIYEKMISGMYLGEIVRRVLLKMAEEAEFFGDTVPSKLEKPFILRTPDMSAMHHDSTADLRVVATKLKDILEISNTSLKMRKVVVELCDIVATRGARLSAAGILGILKKIGRDTVKEGEENHKSVIAMDGGLFEHYTKFRNAMQTAMDELLGEEASRNIIIKLSNDGSGLGAALLAASHSQYREYAEST</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CV57</accession>
    <name>A0AAD5CV57_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein-tyrosine-phosphatase MKP1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_032799</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006451">
        <property type="protein sequence ID" value="KAI7748878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CV57"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004721">
        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14498">
        <property type="entry name" value="DSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057528">
        <property type="entry name" value="MPK1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007122">
        <property type="entry name" value="Villin/Gelsolin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46381:SF2">
        <property type="entry name" value="MAP KINASE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46381">
        <property type="entry name" value="MKPA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25466">
        <property type="entry name" value="MPK1_gelsolin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00262">
        <property type="entry name" value="GEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="4">
        <location>
            <begin position="130"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="5">
        <location>
            <begin position="189"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="461"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="563"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="95"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="696"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7748878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="696" mass="77701" checksum="C107A269070D592A" modified="2024-05-29" version="1" fragment="single">DDTSPPPPPPPPPHSHLPPPLPTGDTRKKIYSRSVSWAAGKARASLPPLQPLSVTRPKAQEWPTAGSDDLGVWPNITATTPGARLKPPSLPLDFNNNNNNNNNNNSSKGLGKPPREFEFKKDKLAYFDKECSRIIDHVFLGSDAVAKNRDVLRQNGITHVLNCVGFVCPEYFKNDLVYKTLWLQDNPSEDITSILYDVFDYFEDVREQNGRVFVHCCQGVSRSTSLVIAYIMWREGQSFETAFQRVKAARGVTNPNMGFASQLLQCQKRVHGVPVSPNSVLRMYRIAPHSSYAPLHLVPKLLARPGAMALDSRGAFIVNVPSAVYVWVGRKCDSLMIESARLGASQVIRYEKANGPAVTVREGEEPSEFWNAVRGNQDQEQMMVVRDGVGERKVVDYDMDFAVFAKAANTGVVPPFSVSGNDYEMCLPARRSGWERLRMKFATGIMKDLITPSKVTLVPAADSPGSCSSSVKNEDDNSECMSPESFSSYLVHDSRKFDSMSPSISPTTSDYSNSLSFSPTWSDLSASDPSPNHKVGKITHSPTNLVRTWSFTLEDQSMECVEADDEEEGQGEESKFKAENQMQNSNDQTNPDFVLYHWPNMEKVEMGSLGVPDSRSVYITHIVDVVFIWVGREFTDDQIEWQTVGHDFVVKKGLKTSSDIQIVREGEEPEQLWKHLHCFSFQNTAENQDKGKTDIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CVD4</accession>
    <name>A0AAD5CVD4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_019105</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006718">
        <property type="protein sequence ID" value="KAI7747589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CVD4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7747589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="27" mass="3373" checksum="31943DEB9BACEE1D" modified="2024-05-29" version="1">MEKNTVSKERIHCNIRRRLKMRSPEIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2024-10-02" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CVU6</accession>
    <name>A0AAD5CVU6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_018549</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006649">
        <property type="protein sequence ID" value="KAI7747874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052957">
        <property type="entry name" value="Auxin_embryo_med"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32387:SF3">
        <property type="entry name" value="ATP_DNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32387">
        <property type="entry name" value="WU:FJ29H11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7747874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="1306" mass="149117" checksum="3DE8CA94C3812365" modified="2024-05-29" version="1">MESSHPKLNRVRDEIKAKLMNTPIVPCESYKEQKLFCKPNEVGRLKHVFWSILNSAKNQGVSFSNISTHGAYILASSFDQEEYDSILEFLEIEAVDNEWYAKCIAGSDLVRGVSEELYMQLLVFIAENWWSSFYTTNIKNIPSIKYVGMDGKVSLFSISACANKLLAADSNLIYWLINWNTEFHCSNGKFFMPAATQEALGSFTKKHTLKKWLMEEVKVKFVGVSEYADDLIPSLRTDRKLAVTCAHFLYHSKEKNYLWEHEVQNLCSKMPIVDNYGQVTVRRDGVLVPANGSKWVDLIRSNPWRHYNYVELGEDYTRSVTYFGNSASGGDLVSFMKTYVGAADIPFLSPPNAVVPTLSSPLTKTNAFLLLSWLNFLRKKNVRLPERFLSSIKNGSWLKISLSGFSGSRPPSESFMLDSSSGSILQHGSVLVDIPLVDVEFYGEEIRSYKEELKTVGVRFENKEACEFIGERLMSLAASSKLTRDNVFSILKFIQYLGAHYISSTELVNRIKQGRWVRTSQGDKTPDNSVLFSYEWNAASELSNIPFIDREYYGEEILSFKKELKLLGVVVNFDESCYQLVSDNLKSSYSLASLSSEAMLLLLRCIERLRSSDKLVQAVKSIKCVKTNLGYRCPSECFLVTSECEWGCLLQVFGSFPIVDANFYGSSIFSMEKELRKIGVMVDFENAAKEFTRTFKQQASASSIRKENVFSFLECFRKLKKMKVKIPEDLKTCLRKEKWLKTKLSDYKSPNECILFGGDWESITPITALPFIDDNKFYGNGIHDYRAELKLLGVTTEFKDGAKFVAAGLYLPECSSSITPASIYALLDSVKRLQETASEIPEKFLDKLSQKNWLKTYFGYKRPKECLLFSSEWVSFLKRDDGPFIDEQFYGSRIESYKKQLSYLGVIIDVKEGSQVIASYLDSQSNFERIKRLYSYLSEFKWEPTDDDSKQIWLPSGTDNGEWVSPQDCVIHDKNSLFGGLLYVLDTYEYEAKILGFFAGIFNVKFHPSVDDYCKLWKTWEDSGKQITHSECCAFWEFVVKNWNERTEKTFSQSISKLPVSSPTSDGILLSDKRDVFIGDDLFLTDLFQSCSGPIFVWYPQPSSKTLTRAKLVDIYKKLGVRTISESAQKNLSDIDQVKLESVSSKEKIMKKGLVKLILGFLADPNLKLETERRHEAISRMLGLEAFETVDPMTVKYGLSLSTGDVLTVEAKRMIRWDKQNSKLFMQKMEEGGGQKSIIEYASHFGEVIAEGMLWENEEVVPQLSELIRLGYLVEFDEEAIDFLMKMKNLQIFVEDQHYLSSIFSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CWC1</accession>
    <name>A0AAD5CWC1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein EI24-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_024113</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006510">
        <property type="protein sequence ID" value="KAI7748545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CWC1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059112">
        <property type="entry name" value="CysZ/EI24"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21389:SF0">
        <property type="entry name" value="ETOPOSIDE-INDUCED PROTEIN 2.4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21389">
        <property type="entry name" value="P53 INDUCED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07264">
        <property type="entry name" value="EI24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="135"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7748545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="228" mass="25807" checksum="11A6C2996073E185" modified="2024-05-29" version="1">MEALWFYPLYIFSFILSTIWYNDIAQFGLTVTGKDAPTNLAPSSQKEPSTSQNATHVDKPTDLGRVMIGIAEQGYSILLLTFFFLEVYLTGFVPIIGKALNFVLLSWMYAYYCFEYKWNFSGLSLDKRLDFFESNWAFFAGFGSPCVLAIFFFSPLVSYGVMAVLFPMFVLTAAGSDAESVINAHKTKWRGEEFGKLPIFYAADKLLMRILSLLPLEPQEQTSDKKAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CWG5</accession>
    <name>A0AAD5CWG5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_024615</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006717">
        <property type="protein sequence ID" value="KAI7747620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CWG5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008390">
        <property type="entry name" value="AWPM-19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33294">
        <property type="entry name" value="AWPM-19-LIKE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33294:SF5">
        <property type="entry name" value="AWPM-19-LIKE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05512">
        <property type="entry name" value="AWPM-19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7747620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="180" mass="19416" checksum="17E1B651531FDF92" modified="2024-05-29" version="1">MAQTVGRNVAAPLLFLNLVMYLITLGFASWCLNKLINRETHHPSFGGNGATEFFLEFAILASVLGIVSKFAGGNHLRAWRNDSLAAAGSTSLVAWAVTLLAFGLACKEINVGGHRGWRLRIVEAFIIILTITELLYVMLVHAGLYSSRYGPGYRDTDYGMGTHPADTGVKETTGVGGTRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CWZ8</accession>
    <name>A0AAD5CWZ8_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_009297</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006523">
        <property type="protein sequence ID" value="KAI7748474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CWZ8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7748474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="95" mass="10445" checksum="533BB7C6121C1B1A" modified="2024-05-29" version="1" fragment="single">QPPIGCGVWVQMKVSTVAARVPLRHLVVAVLNSSSNNFDKDIFSTGYGQASMNQSRVVCLMSVWIRDPQATLDRDLHILKIKPSRPVQASYGVGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CX95</accession>
    <name>A0AAD5CX95_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_002915</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006579">
        <property type="protein sequence ID" value="KAI7748206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CX95"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7748206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="100" mass="11208" checksum="9A761A5DA0ABF4AC" modified="2024-05-29" version="1">MRNPLDLNNLPNDFIKDHSKQPLYDHSSSAASVVVIGCRARMKKMNGNFSFIQEKLLWELLPTGLLYTVLIPPYITSSMQHQQLHQKPQIGSAASSEASL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CXV5</accession>
    <name>A0AAD5CXV5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_005844</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006168">
        <property type="protein sequence ID" value="KAI7750323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CXV5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7750323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="61" mass="6886" checksum="66B118F6FBFD8218" modified="2024-05-29" version="1">MVCKLEDMHLHNKLSGHIHGKSKLSKFYQATLRSQRKVMPGSVTMVVFAFAHKPMAASVFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CY53</accession>
    <name>A0AAD5CY53_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Saccharopine dehydrogenase NADP binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_021924</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006341">
        <property type="protein sequence ID" value="KAI7749557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CY53"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005097">
        <property type="entry name" value="Sacchrp_dh_NADP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43796">
        <property type="entry name" value="CARBOXYNORSPERMIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43796:SF2">
        <property type="entry name" value="CARBOXYNORSPERMIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03435">
        <property type="entry name" value="Sacchrp_dh_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Saccharopine dehydrogenase NADP binding" evidence="1">
        <location>
            <begin position="31"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7749557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="405" mass="43730" checksum="ADC027129AEB12A3" modified="2024-05-29" version="1">MACTYMRNWSSIRVPTVASATLLTENLNSRVLVLGGTGRIGGSTAIALSNLSPNLHIIIAGRNREKGAGMIATLGKNAEFCEFDINDVNSLESALADVDLVVHAAGPFQQTENCRVLEAAIRAKTAYLDVCDDTSYALRAKSFMNEALAAKVPAITTGGIYPGVSNVMAAELVRVAKIENKGEPERLRFYYYTAGTGGAGPTILATSFLLLGEEVIAFNKGEKIKLRPYSGMVNIDFGKGIGKKDVYLLNLPEVTSTHEILGVPTVSARFGTAPFFWNWAMDAMTRFLPSEVLRDRSRVQEMVRLFDPVVRAIDGYAGERVSMRVDLECSGGRQTIGVFSHKRLSISVGYSTAAFALAILEGNTQPGVWFPEEPEGIAIEARELLLERAAQGTINFGSMDGGNKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CYB9</accession>
    <name>A0AAD5CYB9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_000119</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006501">
        <property type="protein sequence ID" value="KAI7748580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CYB9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046959">
        <property type="entry name" value="PRK1-6/SRF4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48007">
        <property type="entry name" value="LEUCINE-RICH REPEAT RECEPTOR-LIKE PROTEIN KINASE PXC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48007:SF39">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000654">
        <property type="entry name" value="Integrin-linked_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="240"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7748580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="270" mass="29999" checksum="F1B967C425A3515E" modified="2024-05-29" version="1" fragment="single">DARWMNTEEFCRHVEVIGRLRHRNLVALRAYLQAREERLLVFDYFPNGSLFSLIHGSKTSSGGKPLHWTSCLKIAEDLATGLLHIHQTPGGLSHGNIKSSNVLLGPDFESCFTDYGLISFKNPNSDDSSAVSLFYHAPECRDFQTPPSQQADVYSFGVLLLELLTGKAPYQDLVADHGSDLPTWVKSIRQKETESSGEPASLAKLEALLNIAVACVSVAPENRPVMREVLRMIKETRMEPVHVSSNSSDHSPGRWSDTVQSLPRDDQLSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CZ95</accession>
    <name>A0AAD5CZ95_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_013021</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006093">
        <property type="protein sequence ID" value="KAI7750719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CZ95"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006597">
        <property type="entry name" value="Sel1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36792">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36792:SF5">
        <property type="entry name" value="SEL1 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08238">
        <property type="entry name" value="Sel1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00671">
        <property type="entry name" value="SEL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7750719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="103" mass="11629" checksum="CFE71F81AD924498" modified="2024-05-29" version="1">MENENNSSGGSGEKRVPLSDVVSDCLRRWFQDTLKEAKAGDLSMQVLLAQMYFNGYGIPKDHQKARIWMTRAARVRSSVWRVSSKRPGYNASDSDSEELLDDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D004</accession>
    <name>A0AAD5D004_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_020499</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005982">
        <property type="protein sequence ID" value="KAI7751094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D004"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7751094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="155" mass="17856" checksum="5675EA5B39DD17B7" modified="2024-05-29" version="1" fragment="single">MGKLLSTTSTPLLFQSKLLCFSLSYLFLSLFFSLYTSLSSTTKCLFRSSPFDPIHSPLFTYPPSYGQHKHPVPSLHPSCNSPVFFSDYWFVFNEIRRIVDDSSWDLKGLKYVHGDGNGERFGRNYSMHERIKYFDYGDGAIEIPCGFFKKFPISD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D217</accession>
    <name>A0AAD5D217_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_008703</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006134">
        <property type="protein sequence ID" value="KAI7750530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D217"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051837">
        <property type="entry name" value="SortingNexin/PXDomain-PKLike"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22999:SF35">
        <property type="entry name" value="PHOX DOMAIN, SORTING NEXIN, PX DOMAIN SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22999">
        <property type="entry name" value="PX SERINE/THREONINE KINASE PXK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PX" evidence="3">
        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7750530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="120" mass="14049" checksum="CFA4BD35F719724F" modified="2024-05-29" version="1" fragment="single">VLGAYYENLDSKSFAVYSIAVTDADNKTWLVKRRYRNFDRLHQYLKNLPNYKLQLPPKRIFSSSTEDAFVHKRCIQLNKYLQDLLSIANIAEQQEVWDFLSMSSKKYSFGRSSSVVRTLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D3U2</accession>
    <name>A0AAD5D3U2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cytosol aminopeptidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_001332</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>Release of N-terminal proline from a peptide.</text>
            <dbReference type="EC" id="3.4.11.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.</text>
            <dbReference type="EC" id="3.4.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homohexamer (dimer of homotrimers).</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M17 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005713">
        <property type="protein sequence ID" value="KAI7752254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D3U2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00433">
        <property type="entry name" value="Peptidase_M17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000033">
        <property type="entry name" value="M17 leucyl aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.220.10">
        <property type="entry name" value="Leucine Aminopeptidase, subunit E, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00181">
        <property type="entry name" value="Cytosol_peptidase_M17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011356">
        <property type="entry name" value="Leucine_aapep/pepB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043472">
        <property type="entry name" value="Macro_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000819">
        <property type="entry name" value="Peptidase_M17_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023042">
        <property type="entry name" value="Peptidase_M17_leu_NH2_pept"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008283">
        <property type="entry name" value="Peptidase_M17_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002076">
        <property type="entry name" value="PRK00913.2-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11963:SF23">
        <property type="entry name" value="CYTOSOL AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11963">
        <property type="entry name" value="LEUCINE AMINOPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00883">
        <property type="entry name" value="Peptidase_M17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02789">
        <property type="entry name" value="Peptidase_M17_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00481">
        <property type="entry name" value="LAMNOPPTDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52949">
        <property type="entry name" value="Macro domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00631">
        <property type="entry name" value="CYTOSOL_AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="Cytosol aminopeptidase" evidence="10">
        <location>
            <begin position="380"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS00631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7752254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="529" mass="55830" checksum="A4D494584DA0FF62" modified="2024-05-29" version="1">MAHDLAKATLGLTLPLQIDPPKISFTAKEINIVEWKGDMLAVGVTEKEMTKDGNSKFQNSILKKIDSQHGGLLFQVSTEEDFTGKLGQSTVLRLSGHGTKRVGLFGLGKCTTGSSTAAYQALGESIASSAKSYQANNVAVIIASSEDLTPALKLTAASAIATGTLHGTYEDNRFRSESKKPTLKSVDFLGLGVGPELEKKLKYTEQVFTGVVLGKELVNSPANVLTPGALAQEAEKIASTYSDVFSVKILDAEQCKELKMGSYLGVAAASANPPKFIHLCYKPTSVNVKKKLALVGKGLTFDSGGYNIKTGPGCSIELMKYDMGGSAAVLGAAKALGQIKPPGVEVHFIVAACENMISGTGMRPGDIVTASNGKTIEVNNTDAEGRLTLADALVYACNLGVDKIVDLATLTGACVIALGSSIAGIFTPSDELAKEVVTASESAGEKLWRMPMEESYWEKMKSSVADMANSGGRQGGSILAALFLQQFVDEKVQWLHIDMAGPVWNDKKKSATGFAIPTLVEWVVSNSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D4Q3</accession>
    <name>A0AAD5D4Q3_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_020135</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005475">
        <property type="protein sequence ID" value="KAI7753314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D4Q3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042139640" evidence="1">
        <location>
            <begin position="21"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7753314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="117" mass="13408" checksum="9EB1AB37B07ED9F2" modified="2024-05-29" version="1">MGKHAALFFTIFILVAFIHNHFTTAPTKIPNQLVEEEEEEKFQEQGMGLSMNSIFRYFGSGRIDFKTLEIGPVSVTLSIEFEERCQQQEEGTEELCTIGFPTIRFRLSINSIPRLFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D4R9</accession>
    <name>A0AAD5D4R9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">ATP synthase subunit beta</fullName>
            <ecNumber evidence="18">7.1.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">M8C21_024799</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="21">AA19_3_7</strain>
            <tissue evidence="21">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.</text>
    </comment>
    <comment type="function">
        <text evidence="18">Produces ATP from ADP in the presence of a proton gradient across the membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>ATP + H2O + 4 H(+)(in) = ADP + phosphate + 5 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:57720"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.1.2.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="18">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) and CF(0) have multiple subunits.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a, b and c.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.1.2.2" evidence="18"/>
    <dbReference type="EMBL" id="JAMZMK010003901">
        <property type="protein sequence ID" value="KAI7754283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D4R9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042776">
        <property type="term" value="P:proton motive force-driven mitochondrial ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18110">
        <property type="entry name" value="ATP-synt_F1_beta_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18115">
        <property type="entry name" value="ATP-synt_F1_beta_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01133">
        <property type="entry name" value="F1-ATPase_beta_CD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1140.10:FF:000001">
        <property type="entry name" value="ATP synthase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.170:FF:000006">
        <property type="entry name" value="ATP synthase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000026">
        <property type="entry name" value="ATP synthase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.910">
        <property type="entry name" value="ATP synthase, F1 beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1140.10">
        <property type="entry name" value="Bovine Mitochondrial F1-atpase, Atp Synthase Beta Chain, Chain D, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01347">
        <property type="entry name" value="ATP_synth_beta_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055190">
        <property type="entry name" value="ATP-synt_VA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042079">
        <property type="entry name" value="ATP_synt_F1_beta_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005722">
        <property type="entry name" value="ATP_synth_F1_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050053">
        <property type="entry name" value="ATPase_alpha/beta_chains"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036121">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024034">
        <property type="entry name" value="ATPase_F1/V1_b/a_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01039">
        <property type="entry name" value="atpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15184">
        <property type="entry name" value="ATP SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15184:SF80">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT BETA-1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22919">
        <property type="entry name" value="ATP-synt_VA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039072">
        <property type="entry name" value="ATPase_subunit_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47917">
        <property type="entry name" value="C-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50615">
        <property type="entry name" value="N-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16 18" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="9 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="15 18" id="KW-0139">CF(1)</keyword>
    <keyword evidence="7" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="6 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="11" id="KW-1278">Translocase</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="20">
        <location>
            <begin position="218"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="44"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU003553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="KAI7754283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="550" mass="59052" checksum="5885050432CC8DCC" modified="2024-05-29" version="1">MASRRLAASLLRSSLHRSRSPITTSTKTTRALSQPHLFNRVVPHSTSSAAAAQSKPPPTTTGAGSTTGKITDEFTGAGSIGQVCQVIGAVVDVRFSEGLPPILTALEVLDNSIRLVLEVAQHLGENMVRTIAMDGTEGLVRGQRVLNTGSPITVPVGRATLGRIINVIGEPIDHRGEIKTDHYLPIHREAPAFVEQATEQQILVTGIKVVDLLAPYQRGGKIGLFGGAGVGKTVLIMELINNVAKAHGGFSVFAGVGERTREGNDLYREMMESGVIKLGDKQSESKCALVYGQMNEPPGARARVGLTGLTVAEHFRDAEGQDVLLFIDNIFRFTQANSEVSALLGRIPSAVGYQPTLATDLGGLQERITTTKKGSITSVQAIYVPADDLTDPAPATTFAHLDATTVLSRQISELGIYPAVDPLDSTSRMLSPHILGEDHYNTARGVQKVLQNYKNLQDIIAILGMDELSEDDKLTVARARKIQRFLSQPFHVAEVFTGAPGKYVELKESIGSFQGVLDGKYDDLPEQSFYMVGGIDEVIAKAEKIAKENA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D4W5</accession>
    <name>A0AAD5D4W5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NAB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_003282</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the NET family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005516">
        <property type="protein sequence ID" value="KAI7753180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D4W5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011684">
        <property type="entry name" value="NAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051861">
        <property type="entry name" value="NET_actin-binding_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32258:SF26">
        <property type="entry name" value="KINASE INTERACTING (KIP1-LIKE) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32258">
        <property type="entry name" value="PROTEIN NETWORKED 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51774">
        <property type="entry name" value="NAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAB" evidence="5">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="273"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="156"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="222"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7753180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="352" mass="41036" checksum="BBDCEE6355EFB19C" modified="2024-05-29" version="1">MEYYLRRLRLSRHDGVKAGSSVSLHGYYYQFLMEDKIQAINGEDRPDTFGERADFYYRKRPELLALVQDLYNRYLYLADRYTRTLSKQQHAHVQELELELEQICDNYTSPSDNAESSLSYQPQKPDPSELIICELVMKFVEYEIVVDELQTMDMVQEESKKKIELQQSLLEVLESERVVLTNENSRLASESLFMKRKAGELARCVLLERSEDQRVFILSRKIDDLQGQIYELEKRNKEYYERLMKQQVEGRDNEGKKNNISINIKRLLVKNNGGGSSESISWSGEDETGWSMSSTSNSSTCTSLAQVMKEKNNKKKMHLPRGRDGGKKGYGWWDRVKKFDMFMCGPHVDATC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D6M9</accession>
    <name>A0AAD5D6M9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">M8C21_009595</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">AA19_3_7</strain>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010004554">
        <property type="protein sequence ID" value="KAI7754080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D6M9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071244">
        <property type="term" value="P:cellular response to carbon dioxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902456">
        <property type="term" value="P:regulation of stomatal opening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009637">
        <property type="term" value="P:response to blue light"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010114">
        <property type="term" value="P:response to red light"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001659">
        <property type="term" value="P:temperature homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd13999">
        <property type="entry name" value="STKc_MAP3K-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000034">
        <property type="entry name" value="Kinase suppressor of Ras 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000310">
        <property type="entry name" value="Serine/threonine-protein kinase HT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF280">
        <property type="entry name" value="PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000654">
        <property type="entry name" value="Integrin-linked_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="34"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAI7754080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="338" mass="37571" checksum="AF3E941C29A05173" modified="2024-05-29" version="1">MEKNKNKTPDSAATTTTTVRPPKRQEWEIDPSKLIIKSVLARGTFGTVHRGLYDGLDVAVKLLDWGEEGHRSEAEIQALRAAFTQEVVVWHKLDHPNVTKVFIGATMGSSDLQIQTENGQIGMPSNICCVVVEYLPGGALKSYLIKNRRKKLPFKVVIQIALDLARGLSYLHSQKIVHRDVKTENMLLDKTRTVKIADFGVARVEASNPNDMTGETGTLGYMAPEVLNGNPYNRKCDVYSFGICLWEIYCCDMPYPDLSFSEVTSAVVRQNLRPDIPRCCPSSLSNVMKQCWDANPDKRPEMDAVVTMLEGIDTTKGGGMIPGDQAQGCLCFRKYRGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DA66</accession>
    <name>A0AAD5DA66_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Carboxypeptidase</fullName>
            <ecNumber evidence="10">3.4.16.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_011168</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the peptidase S10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.-" evidence="10"/>
    <dbReference type="EMBL" id="JAMZMK010000635">
        <property type="protein sequence ID" value="KAI7756014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DA66"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000030">
        <property type="entry name" value="Carboxypeptidase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11320">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018202">
        <property type="entry name" value="Ser_caboxypep_ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF460">
        <property type="entry name" value="CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00131">
        <property type="entry name" value="CARBOXYPEPT_SER_SER"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 10" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="15"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="994"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7756014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="994" mass="110781" checksum="E0DEBD39AAC2EE6D" modified="2024-05-29" version="1" fragment="single">ISKCGDWIIVHGSFPSKNDENNASPRAENPTPAGDSKKRTFATAKVLTHPTHSINIKLTYGFVVDLLVLVSCYGGDGYDPLENALKIQRSRRLVPKHVSNEDHLPEHSTLPVYIGPQDGLKDVDKISILPGQPAGTDFDQYSGYVTVDPNHGRALFYYLAESPVNSSTNPLVLWLNGGPGCSSFGSGAMMELGPFRVNADNTTLSQNKYAWNNVANVLFLESPAGVGFSYSNTSSDYITDDVQTAKDSYTFLINWLERFPEYKTRDFYITGESYAGHYIPQLAHLILQNNKITNQTVINLKGIALGNAYVDDETANTGMFDYFWTHAIISDEIHEGIISHCNFSEGATLTDACIDYIKQGLAAKSNIFFYDIYTPLCSPFSGSTPSISRFDPCTENYILGYLNSPAVQQSLHAKPVQWESCNYFINTNWKDMPFTVLPVIQDLMASGISVWIFSGDTDGRLSVTSSRYAINKLHTSVKIPWYPWMYQVISYKIIIFLFSPNFPISSNQITLQLSVHFTLLVFVSCYGIDGYKPLESALKIQRSRRWELNQASNVHLAEYSTSPVYMGPQEGLKDADKISSLPGEPAGTDFDQYSGYVTVDPNHERALFYYLAESPVNSSTKPLVLWLTGGPGCSSFGTGAMMELGPFRVTADNKTLSRNLYAWNNVANVLFLESPAGVGFSYSNTSSDYVTGDAQTAKDSYTFLINWLERFPEYKTRDFYIVGESYAGHFVTQLAELILQNNKITNQTVIKLKGIALGNAYLDSETDNTGQFDYLWTHAIISDEIHQGIVSNCDFTEGGTITDACNDYINQTRAAMSNIFEYDIYAPLCSSPFRNSTPSISGFDPCTEVYIRAYLNIPAVQQSLHAKPVEYIACNDFIYTNWTDMPFTVLPLVQDLMANGISVWIFSGDTDGSVPVTTSRYDINKLQTTVKTPWYPWMYQGEVGGYAVGYENLTIWTFRPKLPTRARSSVLHFLLGGKASSQSLAKSTRIAQRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DAS6</accession>
    <name>A0AAD5DAS6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MSP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_016519</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the VAMP-associated protein (VAP) (TC 9.B.17) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000097">
        <property type="protein sequence ID" value="KAI7757628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DAS6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090158">
        <property type="term" value="P:endoplasmic reticulum membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061817">
        <property type="term" value="P:endoplasmic reticulum-plasma membrane tethering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000813">
        <property type="entry name" value="Vesicle-associated protein 1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000535">
        <property type="entry name" value="MSP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008962">
        <property type="entry name" value="PapD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016763">
        <property type="entry name" value="VAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10809:SF148">
        <property type="entry name" value="OS01G0936800 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10809">
        <property type="entry name" value="VESICLE-ASSOCIATED MEMBRANE PROTEIN-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00635">
        <property type="entry name" value="Motile_Sperm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019693">
        <property type="entry name" value="VAMP-associated"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49354">
        <property type="entry name" value="PapD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50202">
        <property type="entry name" value="MSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="297"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="MSP" evidence="5">
        <location>
            <begin position="5"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="126"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="241"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="167"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="207"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="220"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7757628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="319" mass="35477" checksum="A20FDB721E5F7770" modified="2024-05-29" version="1">MGAKLLEIDATAIKFVCEVKKQSTSSVHLSNISDEYVAFKVKTTSPKRFCVRPNSGIISPKSACDFTVTMQGLRSNPSEVECKDKFLIQSTVVDAATKEEEVTLELFSKRSGKYVEEKKLMVVLVSKQDSSSEEDKNKVSGKEPTNVSLTREENAVKLGKKPSNESSVKEENVVKTKVLGKEPSNESSMKEKNVVKKDENALPSHTQTKDRREVKDGNEGNLSKNLTSNPVNVVKEDPERKLRLCKQLRELKLKEATLENQLKEAKSTISSLTCQNSIINEAKETLKLELARRSKKGYPFVFVCIVSIIGLISGYISDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DB94</accession>
    <name>A0AAD5DB94_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Expansin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_009867</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Causes loosening and extension of plant cell walls by disrupting non-covalent bonding between cellulose microfibrils and matrix glucans. No enzymatic activity has been found.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Secreted</location>
            <location evidence="7">Cell wall</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Membrane</location>
            <topology evidence="7">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the expansin family. Expansin A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000341">
        <property type="protein sequence ID" value="KAI7756557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DB94"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009664">
        <property type="term" value="P:plant-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22274">
        <property type="entry name" value="DPBB_EXPA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.10:FF:000001">
        <property type="entry name" value="Expansin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.760:FF:000001">
        <property type="entry name" value="Expansin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.760">
        <property type="entry name" value="Expansin, cellulose-binding-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="entry name" value="RlpA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007118">
        <property type="entry name" value="Expan_Lol_pI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002963">
        <property type="entry name" value="Expansin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007112">
        <property type="entry name" value="Expansin/allergen_DPBB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007117">
        <property type="entry name" value="Expansin_CBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036749">
        <property type="entry name" value="Expansin_CBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009009">
        <property type="entry name" value="RlpA-like_DPBB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31867">
        <property type="entry name" value="EXPANSIN-A15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03330">
        <property type="entry name" value="DPBB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01357">
        <property type="entry name" value="Expansin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01226">
        <property type="entry name" value="EXPANSIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01225">
        <property type="entry name" value="EXPANSNFAMLY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00837">
        <property type="entry name" value="DPBB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="Barwin-like endoglucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49590">
        <property type="entry name" value="PHL pollen allergen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50843">
        <property type="entry name" value="EXPANSIN_CBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50842">
        <property type="entry name" value="EXPANSIN_EG45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0134">Cell wall</keyword>
    <keyword evidence="6 7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041783409" description="Expansin" evidence="7">
        <location>
            <begin position="22"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="Expansin-like EG45" evidence="8">
        <location>
            <begin position="50"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="Expansin-like CBD" evidence="9">
        <location>
            <begin position="174"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7756557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="258" mass="28115" checksum="C55B92FC17407BB6" modified="2024-05-29" version="1">MEVMTLAYFLLLSTILTSVNARIPGVYTGGQWESAHATFYGGSDASGTMGGACGYGNLYSQGYGVNTAALSTALFNNGLSCGACFEIKCANDPQWCHPGSPSIFITATNFCPPNFAQASDNGGWCNPPRTHFDLAMPMFLKIAQYRAGIVPVSYRRVPCRKQGGVRFTINGFRYFNLVLITNVAGAGDITQAWVKGSRTGWMSLSRNWGQNWQSNAVLVGQSLSFRVRGSDRRTSTSYNIVPANWKFGQTFVGKNFRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DCD3</accession>
    <name>A0AAD5DCD3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Transcription factor DIVARICATA-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_021048</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000179">
        <property type="protein sequence ID" value="KAI7757112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DCD3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048262">
        <property type="term" value="P:determination of dorsal/ventral asymmetry"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009908">
        <property type="term" value="P:flower development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000009">
        <property type="entry name" value="transcription factor MYB1R1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000154">
        <property type="entry name" value="Transcription factor SRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006447">
        <property type="entry name" value="Myb_dom_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01557">
        <property type="entry name" value="myb_SHAQKYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44042:SF6">
        <property type="entry name" value="DUPLICATED HOMEODOMAIN-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44042">
        <property type="entry name" value="DUPLICATED HOMEODOMAIN-LIKE SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="87"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="7">
        <location>
            <begin position="87"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="8">
        <location>
            <begin position="95"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="138"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="142"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7757112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="166" mass="19399" checksum="92DF051010C43479" modified="2024-05-29" version="1" fragment="single">MQESKNTKWTKEQNTWFEGALAIFDTETPERWSNVAAFVPGKTESDVIKQYEKLKADINDIEAGLVPDDHNFSNFRKRPSKFRCSDRMRRKGVPWTEEEHRRFLMGLQVHGKGDWRSISLNFVITKTSTQVASHAQKYYARQHSDGKEKRRPSIHDMTMLNPNSPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DDG5</accession>
    <name>A0AAD5DDG5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Phosphoribosylaminoimidazole-succinocarboxamide synthase, chloroplastic</fullName>
            <ecNumber evidence="3">6.3.2.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">SAICAR synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_022294</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate + ATP = (2S)-2-[5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamido]succinate + ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:22628"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58443"/>
            <dbReference type="ChEBI" id="CHEBI:77657"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SAICAR synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.6" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010000809">
        <property type="protein sequence ID" value="KAI7755810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DDG5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009570">
        <property type="term" value="C:chloroplast stroma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004639">
        <property type="term" value="F:phosphoribosylaminoimidazolesuccinocarboxamide synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01414">
        <property type="entry name" value="SAICAR_synt_Sc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000199">
        <property type="entry name" value="Phosphoribosylaminoimidazole-succinocarboxamide synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000015">
        <property type="entry name" value="Phosphoribosylaminoimidazole-succinocarboxamide synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00137">
        <property type="entry name" value="SAICAR_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028923">
        <property type="entry name" value="SAICAR_synt/ADE2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018236">
        <property type="entry name" value="SAICAR_synthetase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009251">
        <property type="entry name" value="PRK12607.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43700">
        <property type="entry name" value="PHOSPHORIBOSYLAMINOIMIDAZOLE-SUCCINOCARBOXAMIDE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43700:SF1">
        <property type="entry name" value="PHOSPHORIBOSYLAMINOIMIDAZOLE-SUCCINOCARBOXAMIDE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01259">
        <property type="entry name" value="SAICAR_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01057">
        <property type="entry name" value="SAICAR_SYNTHETASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01058">
        <property type="entry name" value="SAICAR_SYNTHETASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAICAR synthetase/ADE2 N-terminal" evidence="11">
        <location>
            <begin position="106"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00073447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7755810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="388" mass="43659" checksum="4CDB3C2761A26FE5" modified="2024-05-29" version="1">MSSIPTLNPSKTLTIISPSSSSYSTQKPSFLSTQAKTHFKPTQILQKFQSFSIVMATKQQEHEPSLNGLINSDHRDEVIIAAKNALSNCLSETNLHLHIPSLKSKTRGKVRDVYDGGDYLVLVTTDRQSAFDRVLASIPFKGQVLNDTSLWWFNQTQHIAPNAIIAVPDPNVTIAKKCSVFPVEFVVRGYVTGSTDTSLWTVYKNGVRNYCGNVLPEGMVKNERLPANIITPTTKAADHDVPITPEEIVQQGLMSQADYDEASSKALSLFEYGQRVALEHGVILVDTKYEFGKGPDGSVLLIDEVHTPDSSRYWLAHSYESRFQNGLEPENVDKEFLRLWFKSHCNPYEDKVLPDAPEDLVTELAWRYIFLFETITRSRFQIPETMIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DDN4</accession>
    <name>A0AAD5DDN4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MATH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_003194</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000116">
        <property type="protein sequence ID" value="KAI7757462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DDN4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00121">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.210.10">
        <property type="entry name" value="Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2, Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002083">
        <property type="entry name" value="MATH/TRAF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008974">
        <property type="entry name" value="TRAF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46162">
        <property type="entry name" value="TRAF-LIKE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46162:SF40">
        <property type="entry name" value="TRAF-LIKE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22486">
        <property type="entry name" value="MATH_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00061">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50144">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MATH" evidence="1">
        <location>
            <begin position="10"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="MATH" evidence="1">
        <location>
            <begin position="167"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7757462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="304" mass="34899" checksum="66245965B2159DE2" modified="2024-05-29" version="1">MFISKRKHEPAHYMLNIESFSILSEAKTSKIESDVFEASGHKWRLDLYPNGNDDENGAGHISLYVVICDTESCSKGWEVCVDVCFFVYDHIHQNYVTFQDVNGRRTRFHETNMTWGFDKLIPLESFVESKNGYLLNDSCVFGVEVFAVSEFTKMDRCLLMTKPPITMNTHTWTINYFSGVKENILYSEVFKVGKVKWKLILYPKGNKSAEGIHLSIYLGVHEAASFPDGWKIYAKFKLRLKKQGIGSDIEKEIENWFCNSADDWGFGSFLLLSELNDSENGFLLNDNLTVEVEISVMGTLKYFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G300</accession>
    <name>A0AAD5G300_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Histone H2B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">M8C21_006132</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="15">AA19_3_7</strain>
            <tissue evidence="15">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the histone H2B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011541">
        <property type="protein sequence ID" value="KAI7726760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G300"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22910">
        <property type="entry name" value="HFD_H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000014">
        <property type="entry name" value="Histone H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000558">
        <property type="entry name" value="Histone_H2B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055333">
        <property type="entry name" value="HISTONE_H2B_site"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23428">
        <property type="entry name" value="HISTONE H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00621">
        <property type="entry name" value="HISTONEH2B"/>
    </dbReference>
    <dbReference type="SMART" id="SM00427">
        <property type="entry name" value="H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00357">
        <property type="entry name" value="HISTONE_H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5 12" id="KW-0158">Chromosome</keyword>
    <keyword evidence="9 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="11 12" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="10 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="14">
        <location>
            <begin position="36"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="11"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="KAI7726760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="139" mass="15233" checksum="BDFCBF1C1925FF0D" modified="2024-05-29" version="1">MAPKATAGKKPVAEKAPAAEKKPKAEKKVPKDTSATATDKKKKRHKKSIETYKIYIFKVLKQVHPDIGISGKAMGIMNSFINDIFEKLAQEAARLARYNKKNTLSSREIQTAVRLVLPGELAKHAVSEGTKAVTKFTSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G3M3</accession>
    <name>A0AAD5G3M3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Plastid lipid-associated protein/fibrillin conserved domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_019333</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011345">
        <property type="protein sequence ID" value="KAI7727584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G3M3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009536">
        <property type="term" value="C:plastid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039633">
        <property type="entry name" value="PAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006843">
        <property type="entry name" value="PAP/fibrillin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31906">
        <property type="entry name" value="PLASTID-LIPID-ASSOCIATED PROTEIN 4, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04755">
        <property type="entry name" value="PAP_fibrillin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0934">Plastid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Plastid lipid-associated protein/fibrillin conserved" evidence="4">
        <location>
            <begin position="70"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7727584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="242" mass="26697" checksum="8E1E4F9A72601748" modified="2024-05-29" version="1">MASSTAFSLASLSPVNTELSVTPIRTQSRTVCDLRLRNCRSKRNVIISASVSFPAQEVSPSQRRPDELAASIFSKVTQTDRGVTLTKEQHLQVAELTSELSKYCIDSPVTCPLIFGEWDVVYCSNPTSPGGGFRSGFGRLIFKTNEMIQVVEAPDIVRNKVSFSALGFLDGQVSLKGKLIVLDEKWIKVVFEPPQLKLGSAEFQFGGESEVQLEITYIDDKLRLGKGSRGSVFVFQRRNPTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G794</accession>
    <name>A0AAD5G794_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UBA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_008645</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010525">
        <property type="protein sequence ID" value="KAI7731169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G794"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000058">
        <property type="term" value="P:regulation of ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14291">
        <property type="entry name" value="UBA1_NUB1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039749">
        <property type="entry name" value="NUB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12948:SF3">
        <property type="entry name" value="NEDD8 ULTIMATE BUSTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12948">
        <property type="entry name" value="NEDD8 ULTIMATE BUSTER-1 BS4 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22562">
        <property type="entry name" value="UBA_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBA" evidence="1">
        <location>
            <begin position="222"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="1">
        <location>
            <begin position="294"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="1">
        <location>
            <begin position="354"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7731169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="456" mass="51307" checksum="B4A1C630E66B5A83" modified="2024-05-29" version="1">MELGLKNNSKILVTRVSPDQGKELVAEEEKSTRLQRLKAAATSLAKRHADGSLPVEDFNLELENQSGEKVKLGTEDDQRALMMGLMFHGKAKRLVKSKKYEDALEVLTMGEEAFSLCDSSVVKLIDNVPILQIDMVWCYFMLKDISKLSVAGKRLEEARKGIERSHGKESTRLRLLHGNAHPELALHLRLELLEGIVAYHSGDLEKAKRSLNSARSRYLQLQVPDEALSMLMGMGYGERAAKRALRMNGQSVERAVDFLVEEKAKKQQKIEDDIRRRNEIMEQKKFGVTPLKKAVDLQKLKELVSIGFEKELAAESLRRNENEMQKALDDLTNPDTNSDIQLFIESRKKKRARKASDASIERLVTLGFPREAVVAAVDAHGTEELALNHLLEQNGGSGTVANNGDVPIFDQDMERDVEMEDEITGDLQSEDAFSDYDMELTRESEAIEEYLALVLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G7L0</accession>
    <name>A0AAD5G7L0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">40S ribosomal protein S25</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_018569</name>
        <name evidence="7" type="ORF">M8C21_027335</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS25 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010012125">
        <property type="protein sequence ID" value="KAI7724949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="JAMZMK010010786">
        <property type="protein sequence ID" value="KAI7730376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G7L0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.63.20:FF:000001">
        <property type="entry name" value="40S ribosomal protein S25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.63.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004977">
        <property type="entry name" value="Ribosomal_eS25"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12850">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03297">
        <property type="entry name" value="Ribosomal_S25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="22"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU366057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7724949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7730376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="108" mass="11965" checksum="F03AF7363B21EF05" modified="2024-05-29" version="1">MAPKKEKAPPPSSKPAKSGGGKQKKKKWSKGKQKEKVNNMVLFDKATYDKLLSEAPKFKLITPSILSDRLRINGSLARKAIKDLMARGAIRMVSVHASQQIYTRATNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G8A5</accession>
    <name>A0AAD5G8A5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">E2 ubiquitin-conjugating enzyme</fullName>
            <ecNumber evidence="1">2.3.2.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_006549</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="1"/>
    <dbReference type="EMBL" id="JAMZMK010010526">
        <property type="protein sequence ID" value="KAI7731141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G8A5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd23790">
        <property type="entry name" value="UBCc_UBE2A_2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000017">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 8" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="9">
        <location>
            <begin position="4"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="7">
        <location>
            <position position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00056190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7731141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="152" mass="17312" checksum="788AB820ACCED2C0" modified="2024-05-29" version="1">MSTPSRKRLMRDFKRLQQDPPAGISGAPYDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFSEDYPNKPPTVRFISRMFHPNIYADGSICLDILQNQWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARLFSENKREYNRKVKEVVEQSWTAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G8C4</accession>
    <name>A0AAD5G8C4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TFIIS central domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_006105</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010280">
        <property type="protein sequence ID" value="KAI7732222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G8C4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21538">
        <property type="entry name" value="SPOC_TFIIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.30">
        <property type="entry name" value="Transcription elongation factor S-II, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012921">
        <property type="entry name" value="SPOC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003618">
        <property type="entry name" value="TFIIS_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036575">
        <property type="entry name" value="TFIIS_cen_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11477:SF20">
        <property type="entry name" value="SPOC DOMAIN _ TRANSCRIPTION ELONGATION FACTOR S-II PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11477">
        <property type="entry name" value="TRANSCRIPTION FACTOR S-II ZINC FINGER DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07744">
        <property type="entry name" value="SPOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07500">
        <property type="entry name" value="TFIIS_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00510">
        <property type="entry name" value="TFS2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46942">
        <property type="entry name" value="Elongation factor TFIIS domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51321">
        <property type="entry name" value="TFIIS_CENTRAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TFIIS central" evidence="2">
        <location>
            <begin position="217"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="116"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="213"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="364"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="447"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="683"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="775"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="218"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="370"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7732222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="891" mass="98890" checksum="29F69B18ECFA7D95" modified="2024-05-29" version="1">MLNNFHSHTSIISFWKMESVWNNTGSPKPLLPTKRKAATEPLSPSVVEKRVGHNEPYVNKRPTPLQPVLSKSASQRVQTPKNRTAIHEVSPKVQSGSFEAVRSKMRETLAAALSVGIQNQEKSPNEHNKSPSEAVKAQADIQPDGSTANVGKFEDRATTEGQNGKHDEQEPQYNYVMPDADSSFGDTFFVKDELLQGNGLSWAWDMEVTDSKEVSGVEQGQSGDLTGSENNIKSPEELASRIEAELFKLFGGVNKKYKEKGRSLMFNLKDPNNPELREKVLSGVISPERLCSMTPEELASKQLSEWRMAKAEEHDKMIVLPESDVDIRRLVKKTHKGEYQVEVEQDDAVSVEVSVGSTSFTQFQPKKKKTEHEVKGKEVADDDKSRSENLTIPADGTDFMQDIIVDEFKDEGFLPPIVSLDEFMESLNSEPPFEDLPVDSKETKVVNSDTVDETDSEKLSSVPASAGAVDQPKTKSNEAVLSVKKTSDTNVERKAVSASSAVIGEHVWEGELQLTISSSISAVGLFRSGEKTSTKEWPGSMEIKGRVRLDAFEKFLQELPMSRTRAVMVVHFVLKDPSSENHRAMLSEAVESYIADERLGFGEPNPGVELYFCPPHTRIIEMLCKHLAKNQTDILKSTDNGLIGVVVWRRPHISLPNSSHQHKHHKKHNVSTRRHENINANTSLNFTPKSVNRMPQKEVSNNDDDDDDIPPGFGPGAAAAAAARDEDDLPEFNFTGSSSGQKIPGQVGFGSKQAQSNPSRPVEQMRQLIYRYGHTASGGEGTINKPWNKDDEDDDMPEWQPHQHQNQHGPEVPLPYMQQPVGPHMVNQIRPPVGQVMRPPMTIQPQANVLQNVVGWPPPPPGPHMLPPNAAVPGQFYDGQWRLDAPRSRGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G8I8</accession>
    <name>A0AAD5G8I8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-CH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_033495</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010092">
        <property type="protein sequence ID" value="KAI7733090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G8I8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16495">
        <property type="entry name" value="RING_CH-C4HC3_MARCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46158:SF15">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE MARCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46158">
        <property type="entry name" value="OS02G0165000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12906">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51292">
        <property type="entry name" value="ZF_RING_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="332"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="358"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="387"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="414"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="domain" description="RING-CH-type" evidence="6">
        <location>
            <begin position="233"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="452"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="452"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="474"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="487"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="507"/>
            <end position="519"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7733090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="532" mass="58693" checksum="5BFF7FCF015F75B6" modified="2024-05-29" version="1">MNPQEKNGIKSAGDEVHDSSSSQTKGVAVPFQMGENYLKEHVVLEMPSRTIQSSSSHECVQMQMPPPPPPPLPRFHKRVNFNVAAPPETCSSRNKSLKKNLLPKSSFKNKNTNSDVMISIGPGSLPEEKSSIARSWSLTKMFTKITTPSRPFGLTGLSDSESGLRRAGGSLNLQTKVHDHIYRSQSVPILNEDTHYKRMDSFFRVIPTTPRVKEFDSMKPTPTSTDDDADGDDIAEEEAVCRICLIELCEGGETLKMECSCKGELALAHKECAIKWFSLKGNKTCDVCHQDVENLPVTLLRIQSRNRDNGTTAAHDHHVEVNAAIYRVWKEVPILVIVSTLAYFCFLEQLLVGDMGKSSIAISLPFSCVLGLLSSMTSTAMVKRRFVWLYASIQFAFVVIFGHIFLSVANLRPMLSIILSTFAGCGAAICSRSIVMEALKLHRWWRSISNQQHDSPNVETTPPSQLPALDTPQSLPPPPPPPPPSYIASTNVSPSEPTEHGVASPPQLVPQPPSQPPPTLSQVYQFEVNWKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G981</accession>
    <name>A0AAD5G981_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Calnexin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">M8C21_032242</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">AA19_3_7</strain>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Calcium-binding protein that interacts with newly synthesized monoglucosylated glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">Belongs to the calreticulin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010364">
        <property type="protein sequence ID" value="KAI7731878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G981"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.250.10:FF:000001">
        <property type="entry name" value="Calnexin homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000048">
        <property type="entry name" value="Calnexin homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.250.10">
        <property type="entry name" value="Calreticulin/calnexin, P domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001580">
        <property type="entry name" value="Calret/calnex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018124">
        <property type="entry name" value="Calret/calnex_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009033">
        <property type="entry name" value="Calreticulin/calnexin_P_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11073:SF1">
        <property type="entry name" value="CALNEXIN 14D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11073">
        <property type="entry name" value="CALRETICULIN AND CALNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00262">
        <property type="entry name" value="Calreticulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00626">
        <property type="entry name" value="CALRETICULIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63887">
        <property type="entry name" value="P-domain of calnexin/calreticulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00803">
        <property type="entry name" value="CALRETICULIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00804">
        <property type="entry name" value="CALRETICULIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00805">
        <property type="entry name" value="CALRETICULIN_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="14 17" id="KW-0143">Chaperone</keyword>
    <keyword evidence="12 16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8 17" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0430">Lectin</keyword>
    <keyword evidence="11 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="448"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="202"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="479"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="208"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="234"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="272"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="282"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="483"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="92"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00037525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR601580-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU362126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAI7731878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="21">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="525" mass="59234" checksum="D723277EFFDBC0AD" modified="2024-05-29" version="1">MIGCLFSQIYASSIFYDSFDESFEGSWIVSEKEEYSGEWKHSKSEGHDDYGLLVSQQARKYAIVKELEKPVELKDSTTVLQFEVRLQNGLECGGAYLKYLRPQEAGWTAKGFDNESPYSIMFGPDKCGATNKVHFILKHKNPKSGEYVEHHLKFPPTVPSDKLTHVYTAIIKPDNELVILVDGEEKKKANFLSSEDFEPALIPAKTIPDPDDKKPEDWDERAQIPDPEATKPEDWDEDAPMEILDEEAEKPEGWLEDEPEEIDDPEAVKPEDWDDEEDGEWEAPKIDNPKCEDAPGCGEWRRPLKRNPAYKGKWHAPLIDNPAYKGIWKPQEIPNPDYFELEKPNFEPIAAIGIEIWTMQDGILFDNVLIASDEKTATEIRDTTWKPKFKVEEEKQKAEEQAAGSDGLSGIQKTVFDVLYKIADLPFLGNHKDKVLEVIEKAEKQPNITIGVIVSIVVVIFSVLLKLLFGGKKATKKVNVAPAKKEEGEDSKAAEDEGEEEKEEKSDDGAAAGGAVPRRKTRRAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G9D7</accession>
    <name>A0AAD5G9D7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">4-alpha-glucanotransferase</fullName>
            <ecNumber evidence="4">2.4.1.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Amylomaltase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Disproportionating enzyme</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_007244</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Transfers a segment of a (1-&gt;4)-alpha-D-glucan to a new position in an acceptor, which may be glucose or a (1-&gt;4)-alpha-D-glucan.</text>
            <dbReference type="EC" id="2.4.1.25"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the disproportionating enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.25" evidence="4"/>
    <dbReference type="EMBL" id="JAMZMK010010049">
        <property type="protein sequence ID" value="KAI7733269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G9D7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004134">
        <property type="term" value="F:4-alpha-glucanotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001070">
        <property type="term" value="F:starch binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05816">
        <property type="entry name" value="CBM20_DPE2_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013784">
        <property type="entry name" value="Carb-bd-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002044">
        <property type="entry name" value="CBM20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034841">
        <property type="entry name" value="CBM20_DPE2_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003385">
        <property type="entry name" value="Glyco_hydro_77"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32518">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32518:SF3">
        <property type="entry name" value="4-ALPHA-GLUCANOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00686">
        <property type="entry name" value="CBM_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02446">
        <property type="entry name" value="Glyco_hydro_77"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01065">
        <property type="entry name" value="CBM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49452">
        <property type="entry name" value="Starch-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51166">
        <property type="entry name" value="CBM20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CBM20" evidence="11">
        <location>
            <begin position="69"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="836"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7733269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="836" mass="96032" checksum="73E4B160D747FB0C" modified="2024-05-29" version="1" fragment="single">KNVLRWEGGNKRKLAVPEGVENGKVVEFRDLWLNGSDSLPFKSAFKNVIFRKSSSLAIEKPLGVIHNKLDQDDSVIVQFKICCPNIAEGTSIYVIGSSVELGKWKVHDGLKLSYAGESVWEGDCVVPKAAFPLKYKYCKYGKNGNFSLEYGSNREVTVDISAIGPNYVILSDGMMREMPWRGAGVAIPMFSVRSEDDVGVGEFVDLKLVVDWAVNSGFHLLQLLPINDTSVHKMWWDSYPYSSLSVFALHPLYLRLQRLSDNIPDDINQEIQRAKKELDGKNVDYDATMNTKLSIAKKIFDLEKDLILNSDSFKNFLSKNEEWLKPYAAFCFLRDFFETSDHSQWGTFSQFSKDKLEKLVSKDSFHYDIICFHYYIQYHLHLQLSEAAEYAKEKGVVLKGDLPIGVDKNSVDTWMANYFTAYRIDHILGFFRIWELPAHAMTGLVGKFRPSIPLNQEELEKEGIWDFDRLSKPYIRQEFLQEKFGPLWTVIASYFMNEFKKNHYEFKDDCNTEKKIAAKLKSLIEKKLLLENEDKLRRELFDLVQNIVLIRDPEDAKSFYPRFNLEDTSSFKDLDENRDSLAPKCHEDFACASKFLRYVMQELGLIGLRIQRMPSEADLEFGIPSQYDYMTVCAPSCHDCSTLRAWWEEDEDRRRSFFKTMVGSDSLPPEKCVPEIHVESPSMWAIFPLQDLLALKDEYTTRPATEETINDPTNPKHYWRFRVHVTMESLLKDTDLTTMIKGLVCASARSYPGQEEAQSGVAVVPPKQQIAGEQEKIRLPNQDPKITSIAVLLSEIRDVDLAIDICNLVVYANAQTNARHVTANLLWKDDSSERFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GA50</accession>
    <name>A0AAD5GA50_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_025390</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010097">
        <property type="protein sequence ID" value="KAI7733016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GA50"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7733016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="193" mass="21546" checksum="992C7BB9800F352E" modified="2024-05-29" version="1">MDCSLNSPELGPLDKASSVAEDAMENTPELDPLDKAPLIFVDDVPKFQMKTLPVLEKGVTDECLLNVFVEVKEFSLLPMKILIMLNQCRKALANGEDCLAQELLNQLKMGEYEIVDVDDVKCLTKKYQIKIRGINALDIGVKTREKLVEMVDGKQLTVVVYCFDDSGKHWVGDVYCDGICLLEKLCGSEALRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GAD2</accession>
    <name>A0AAD5GAD2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cyclin-dependent kinase inhibitor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_018983</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the CDI family. ICK/KRP subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009783">
        <property type="protein sequence ID" value="KAI7734214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GAD2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004861">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.365.10">
        <property type="entry name" value="p27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003175">
        <property type="entry name" value="CDI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044898">
        <property type="entry name" value="CDI_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044275">
        <property type="entry name" value="KRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46776">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE INHIBITOR 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02234">
        <property type="entry name" value="CDI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017811">
        <property type="entry name" value="CDK_inhib_pln"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3 5" id="KW-0649">Protein kinase inhibitor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-dependent kinase inhibitor" evidence="6">
        <location>
            <begin position="157"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR017811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7734214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="198" mass="22506" checksum="4793FF5E3251B4D9" modified="2024-05-29" version="1">MGKYMRKPKLTKDTVTIIDVSQSFLGVRTRAKTLALQKQLQAALKPSTLLPPSTAEQQPESCYLQLRNRRLQKPLVEGLTITCCRQHNTNTCYSVGSGSVDQTRIKGGGEIDEGCFGGNDLDFDCRERSTRESTPCSSIKDLNVINTLSNFITRSMPMSHDIEEFFALHEQEQHKRFADKYNFNFVDEKPLEGCYEWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GBR4</accession>
    <name>A0AAD5GBR4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_006005</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009743">
        <property type="protein sequence ID" value="KAI7734368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GBR4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01959">
        <property type="entry name" value="nsLTP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.110.10">
        <property type="entry name" value="Plant lipid-transfer and hydrophobic proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036312">
        <property type="entry name" value="Bifun_inhib/LTP/seed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016140">
        <property type="entry name" value="Bifunc_inhib/LTP/seed_store"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033872">
        <property type="entry name" value="nsLTP2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33214">
        <property type="entry name" value="BIFUNCTIONAL INHIBITOR/LIPID-TRANSFER PROTEIN/SEED STORAGE 2S ALBUMIN SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33214:SF44">
        <property type="entry name" value="NON-SPECIFIC LIPID TRANSFER PROTEIN GPI-ANCHORED 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00234">
        <property type="entry name" value="Tryp_alpha_amyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47699">
        <property type="entry name" value="Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical" evidence="3">
        <location>
            <begin position="16"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7734368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="79" mass="8540" checksum="3EF9BC9AC155E3BF" modified="2024-05-29" version="1">MLVLMGHGSAAVNCNYMELMVCAGAVTSHQPPSSDCCAKVKEQKTCFCGYLQNPTIRQYVTPQDAQRVAKQCGVTLPNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GG07</accession>
    <name>A0AAD5GG07_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_004404</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008534">
        <property type="protein sequence ID" value="KAI7739989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GG07"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045874">
        <property type="entry name" value="LRK10/LRL21-25-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR27009">
        <property type="entry name" value="RUST RESISTANCE KINASE LR10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="1"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7739989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="145" mass="16490" checksum="C34D6F688D618650" modified="2024-05-29" version="1">MISVSCMRGTPGYIAPELFSRNFGQVSHKSDVYSYGMMILEMVGGRRNVETEVDHTSEIYFPQWIYKKVEIEEQLGLHGIVSNEENEMARKMIIVGLWCIQTNPVNRPAITKVLEMLEGSFELLKIPPKPYMCSPQRSGDLSTTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GGB4</accession>
    <name>A0AAD5GGB4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Importin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_010440</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the importin beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008688">
        <property type="protein sequence ID" value="KAI7738973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GGB4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000246">
        <property type="entry name" value="Transportin MOS14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013598">
        <property type="entry name" value="Exportin-1/Importin-b-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051345">
        <property type="entry name" value="Importin_beta-like_NTR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057941">
        <property type="entry name" value="TPR_TNPO3_IPO13_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058537">
        <property type="entry name" value="TPR_TNPO3_IPO13_4th"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12363:SF33">
        <property type="entry name" value="IMPORTIN-13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12363">
        <property type="entry name" value="TRANSPORTIN 3 AND IMPORTIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24138">
        <property type="entry name" value="TPR_TNPO3_IPO13_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24139">
        <property type="entry name" value="TPR_TNPO3_IPO13_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08389">
        <property type="entry name" value="Xpo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="5">
        <location>
            <begin position="26"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7738973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="960" mass="106893" checksum="B4792789880A4347" modified="2024-05-29" version="1">MSHENIVKEALNALYHHPDDAVRSQADQWLQKFQRTIDAWQVSDNLLHDASSNVETLIFCSQTLRSKVQRDFEELPSEAFRPLRDSLNNLLKTFHKGPPKVRTQISLAVAALAVQVPAEDWGDGGIMNWLRDEMNSHPDIVPSFLELLRVLPEEVFNYKIAARPDRRRKFEDELVSTMDVALGILTACLNIPELKEQALEAFASWLRLRHRIPASVLATHALVLTALSSLTSDVLSEAAVNVISELIHYTSARNLDGFSAQMPLIQVIVPQVMNLKPQLRDSSKDEEDVKAIARLFADMGDSYVELIATGSDESMMIVHALLEVAAHPEYDIASMTFNFWHNLQICLIERESYVSIGNESLIEAERNRRLQVFRSSYETLVSLVSCRVQYPQDYSELSKEDQKDFKQTRYAVADILIDAALVLGGETTLKILYMKLVEALSGPQNGDMMDWRPSEAQLYCIRAISELVPITESEVMPQIMSLLPKLPHQSLLLQTVCLTVGAYSKWLDAAPTGLSFLPSVIDILVRGMSMSEDSAAAAALAFRHICDDCGKKLRGSLDGLFQIYQTAMTGEGSFKVAAQDSLHLVEALSIVITELPPDQAKKALEALCIPVVAPLQEVINQGPLVLGQKPAREITVHIDRLANIFRYVDNPEAVADAIQRLWPLFKAIFDIRAWDMRTMESLCRACKHAVRTSKRFMGVTIGAMLEEIQGLYKQHHQPCFLYLSSEVIKIFGSDPSCADYLKVLIESLFSNTACLLTKIQDFTSRPDIADDCFLLASRCIRYCPHLLFPSPVFPSLVECSMIGITVQHREASNSILSFLSDIFDLGKSSQGQQFVSIRDSVIVPRGASLTRILIAAATGALPSSRLETVAYALLALSRAYGVKALEWAKEGVSLIPVTAATDMEKSRFLQSLSDAASGVDIKGLTNPIEELSEVCRRNRTVQEIVQEALRPLELNLVYVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GGM0</accession>
    <name>A0AAD5GGM0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_026581</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008125">
        <property type="protein sequence ID" value="KAI7741865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GGM0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01837">
        <property type="entry name" value="SGNH_plant_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051238">
        <property type="entry name" value="GDSL_esterase/lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035669">
        <property type="entry name" value="SGNH_plant_lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45650">
        <property type="entry name" value="GDSL-LIKE LIPASE/ACYLHYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45650:SF9">
        <property type="entry name" value="SGNH HYDROLASE-TYPE ESTERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042274610" evidence="8">
        <location>
            <begin position="21"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7741865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="362" mass="40522" checksum="A19A20E84EB699C0" modified="2024-05-29" version="1">MALKLCQLPLSLLIIGLAQLETFVGGTTGVPRVPCYFIFGDSLVDCGNNNDLETAAKANYAPYGMDFPTGANGRFTNGLTIADLIGQLLGFDHFIPSYASARDEEISSGVNYGSGSAGIREETGKHLGDRVCMDRQLRHHERITSRIRALQQNKTFTNEHLKECIYIVNMGNNDYINNYLMPQFYKTSHKYRMEQYSEVLAEQYTKQLKKLYRLGARRIAVFGLGMLGCAPALMQRFETNGTCVDWINDAVMLFNNRLHPLVEELNYVYPNARFTFINLTNILAPKGGEPLPNKPCCPVRPDGQCVPNGVCCPSRSLSVYIDSFHPTEIANILLATRAYKAQSNLDVYPMDIGQLTRDRFDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GI48</accession>
    <name>A0AAD5GI48_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_026822</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FPP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008353">
        <property type="protein sequence ID" value="KAI7740728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GI48"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008587">
        <property type="entry name" value="FPP_plant"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31580">
        <property type="entry name" value="FILAMENT-LIKE PLANT PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31580:SF4">
        <property type="entry name" value="FILAMENT-LIKE PLANT PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05911">
        <property type="entry name" value="FPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7740728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="200" mass="22843" checksum="4E9CFA845B3C8BBB" modified="2024-05-29" version="1">MLTIGDDNSVPRWDEDTSTPFQKYRENKRDLTIGYIGDEWDEEYDKGKRKKVRISKTEFDGKNPFQDIANERLKSPPFLELIIHQCTPSLFSGYELRCHKNGVEMAEKSGSKVPSAHGRELNEQLLEIVTQENFVKQHSKVAEGVVSGWEKAEAEAATLKSHLESVTLLKLTAKDRASHLDGALKECMRQITNLKEEHEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GKD4</accession>
    <name>A0AAD5GKD4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">U1 small nuclear ribonucleoprotein C</fullName>
            <shortName evidence="9">U1 snRNP C</shortName>
            <shortName evidence="9">U1-C</shortName>
            <shortName evidence="9">U1C</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_000992</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the spliceosomal U1 snRNP, which is essential for recognition of the pre-mRNA 5' splice-site and the subsequent assembly of the spliceosome. U1-C is directly involved in initial 5' splice-site recognition for both constitutive and regulated alternative splicing. The interaction with the 5' splice-site seems to precede base-pairing between the pre-mRNA and the U1 snRNA. Stimulates commitment or early (E) complex formation by stabilizing the base pairing of the 5' end of the U1 snRNA and the 5' splice-site region.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the U1 snRNP. The U1 snRNP is composed of the U1 snRNA and the 7 core Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP, and at least 3 U1 snRNP-specific proteins SNRNP70/U1-70K, SNRPA/U1-A and SNRPC/U1-C. SNRPC/U1-C interacts with U1 snRNA and the 5' splice-site region of the pre-mRNA. Interacts (via N-terminus) with TIA1 (via C-terminus); thereby promoting spliceosomal U1 snRNP recruitment to 5' splice sites.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">U1 snRNP is composed of the 7 core Sm proteins B/B', D1, D2, D3, E, F and G that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP, and at least 3 U1 snRNP-specific proteins U1-70K, U1-A and U1-C. U1-C interacts with U1 snRNA and the 5' splice-site region of the pre-mRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the U1 small nuclear ribonucleoprotein C family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007562">
        <property type="protein sequence ID" value="KAI7744234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GKD4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000243">
        <property type="term" value="C:commitment complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030627">
        <property type="term" value="F:pre-mRNA 5'-splice site binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030619">
        <property type="term" value="F:U1 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000395">
        <property type="term" value="P:mRNA 5'-splice site recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000387">
        <property type="term" value="P:spliceosomal snRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000059">
        <property type="entry name" value="U1 small nuclear ribonucleoprotein C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03153">
        <property type="entry name" value="U1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000690">
        <property type="entry name" value="Matrin/U1-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013085">
        <property type="entry name" value="U1-CZ_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017340">
        <property type="entry name" value="U1_snRNP-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31148">
        <property type="entry name" value="U1 SMALL NUCLEAR RIBONUCLEOPROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31148:SF1">
        <property type="entry name" value="U1 SMALL NUCLEAR RIBONUCLEOPROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06220">
        <property type="entry name" value="zf-U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037969">
        <property type="entry name" value="U1_snRNP-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50171">
        <property type="entry name" value="ZF_MATRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 9" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Matrin-type" evidence="11">
        <location>
            <begin position="4"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="154"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="193"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7744234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="204" mass="22128" checksum="2084D1C58D1577A8" modified="2024-05-29" version="1">MPRYYCDYCDTYLTHDSPSVRKQHNAGYKHKANVRIYYQQYEAQQNQYLIDQKVKEHLGQAAAYQQVGAAYNQLRPRFPVLPTPMMPMPGNPMMQMPGMRPLAPLPRPIPGYPGMPPTLAPPGAPSMPGQVNPSMPGQVNNGVPRPITISAPMMAPGSSGTPTSGVPPPMYTPGMYQANPTAPAPSSGGFESPNASAQPQEAHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GKS5</accession>
    <name>A0AAD5GKS5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Red chlorophyll catabolite reductase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_026967</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007666">
        <property type="protein sequence ID" value="KAI7743831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GKS5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009507">
        <property type="term" value="C:chloroplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051743">
        <property type="term" value="F:red chlorophyll catabolite reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015996">
        <property type="term" value="P:chlorophyll catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1500.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009439">
        <property type="entry name" value="RCC_reductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34685">
        <property type="entry name" value="RED CHLOROPHYLL CATABOLITE REDUCTASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34685:SF2">
        <property type="entry name" value="RED CHLOROPHYLL CATABOLITE REDUCTASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06405">
        <property type="entry name" value="RCC_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7743831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="308" mass="34323" checksum="9376CF5C71EE7C0B" modified="2024-05-29" version="1">MAAITGHLASFSIFSPSPKLPNQPVSSPSPRFSCYCSKSSPQQQQQQMAYMDFPYVSPSHKNLMVDLASTFESRLNSFLNPCNLPPDVQSYQNNTGTAHAALHIRSGLHSSPIDFMLGSWLHCALPSGGALNITSLSAYLNSSTDAPNFLVELIQSSPTSMVLILDLPPRVDLVLNPDYLKTFYEDTQLDQHRQHLDKLSEVKPYFSSSLYIRAVVSPTAILVRIETEQLEEMIKTHVTPIAKEVLKTWLDVCAFGERVVGESERDSLKKRDEMSKSKTIEIDLGSNMPRLFGQETANRVLDALKEVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GPA4</accession>
    <name>A0AAD5GPA4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_008173</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006197">
        <property type="protein sequence ID" value="KAI7750145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GPA4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000051">
        <property type="entry name" value="Receptor-like serine/threonine-protein kinase ALE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000146">
        <property type="entry name" value="receptor-like serine/threonine-protein kinase ALE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057597">
        <property type="entry name" value="ALE2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47989">
        <property type="entry name" value="OS01G0750732 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47989:SF40">
        <property type="entry name" value="RECEPTOR-LIKE SERINE_THREONINE-PROTEIN KINASE ALE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23180">
        <property type="entry name" value="ALE2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="281"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="370"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="651"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="656"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="398"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7750145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="775" mass="84385" checksum="8782B919903B976F" modified="2024-05-29" version="1">MVCLRGVQRLDGGIPFLNINLSPSLLPSQPMFVKKNGFEHLKVRLMSMSSSVVPAITSFALPPEPFTRPSFVPAPSPTIQGPIAGPTVRSSRRHRHRFHRGKITHVIPPSPSPLPGCDQTCTDPLTSTPIGSPCGCVFPMKVRLLLQVSVYAVFPVVNELEIEIAQGTYLTQSQVVITGASADSQDQDKTAVDINLVPLGEKFDNTTALLTYDRFWRKKLALNRTLFGDYEVVYITYPGLPSSPPYGELPGNGPNGSPGNRQYPITATFVGKSQKMNPRTIFVIVLSAVVLLVVCCAAISVFVKYRKIGRSSTAVGPVFTAPTSKRRGFGAMLSSDPSNSTLSVFSAMPTSLLSVKTFTLAELDKATEKFSSRKILGEGGFGCVYHGIIEDGSEVAVKLLNRDNNQNGDREFIAEVEMLSRLHHRNLVKLIGICIEGRTRCLVYELVPNGSVESHLHGVDKQKGPLDWDARLKIALGAARGLAYLHEDSNPRVIHRDFKASNVLLEDDFTPKVSDFGLAREATEGSSHISTRVMGTFGYVAPEYAMTGHLLVKSDVYSYGVVLLELLSGRKPVDMAQPPGEENLVTWARPLLTSREGLQQLVDPNLSGTYDFDDMAKVAAIASMCVHPEVAQRPFMGEVVQALKLIYNDKDEVGGDNSTQRDSSVVDSDFKGDHAPSDSSWWNAGGGATPRLTFGQASSFITMEYSSGPLEDIENRAFSASSFDNRVGVALPISHGNRSGPLRTVRSKPLFYRSKGSMSEHGGLLSKPFWSEASF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GQE1</accession>
    <name>A0AAD5GQE1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">FAD/NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_022556</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Putative FAD-dependent oxidoreductase.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAD-dependent oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005885">
        <property type="protein sequence ID" value="KAI7751482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GQE1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004174">
        <property type="term" value="F:electron-transferring-flavoprotein dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.100:FF:000006">
        <property type="entry name" value="apoptosis-inducing factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43735">
        <property type="entry name" value="APOPTOSIS-INDUCING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43735:SF3">
        <property type="entry name" value="FERROPTOSIS SUPPRESSOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="6">
        <location>
            <begin position="12"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00057036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7751482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="362" mass="39777" checksum="1B829CB283E52460" modified="2024-05-29" version="1">MAENGSEEKKKRVVVIGGGIAGSLLCKTLHKHHHFDFTLIDSKEYFEITWAKLRAMVEPSFAKRTVIDHHEYLPGASIITSDAIGIDEQCQVSTAEGRQIAFDYLVIATGHLTNSHLTKTERLRQFEADYEKIKSSDTILIVGGGPSGVELAGEIVVDFPAKKVKLVHRGSRLLEFISETAGKKALDWLTARNVEVMLGQSVDLDSGSDGVYLTSSGETIVADCHFICTSEPIGSSWMKETILKYSLDDRGRLMVDKNLRVKGFANIFAIGDVTDVPELKQGYLAQKHALVVANNLKLLIRGEEGKMVKYYPATSALAIVSLGRREALAYILCVAMIGRIPGLIKSKDLFVGKTRRQLGLQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GQN8</accession>
    <name>A0AAD5GQN8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein TIFY</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Jasmonate ZIM domain-containing protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_030094</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Repressor of jasmonate responses.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="2">The jas domain is required for interaction with COI1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the TIFY/JAZ family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006415">
        <property type="protein sequence ID" value="KAI7749051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GQN8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031347">
        <property type="term" value="P:regulation of defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000022">
        <property type="term" value="P:regulation of jasmonic acid mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009611">
        <property type="term" value="P:response to wounding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040390">
        <property type="entry name" value="TIFY/JAZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010399">
        <property type="entry name" value="Tify_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33077">
        <property type="entry name" value="PROTEIN TIFY 4A-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33077:SF8">
        <property type="entry name" value="PROTEIN TIFY 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06200">
        <property type="entry name" value="tify"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00979">
        <property type="entry name" value="TIFY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51320">
        <property type="entry name" value="TIFY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1184">Jasmonic acid signaling pathway</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tify" evidence="4">
        <location>
            <begin position="271"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="48"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="98"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="210"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="312"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="54"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="210"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="255"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="312"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="341"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU369065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7749051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="356" mass="37604" checksum="741FAF715A8C9C0A" modified="2024-05-29" version="1">MAATSLLNIMAQHNNNNAASEDDVKPTILHDFLGRDTRCAPAAKFVAASDGSSASGGGARGGGPASTASDLGSERHAGTLFEGVPFFGHKTDLSSSKIRYSGSKRSHSDSGFLPPSREVIPQLRPDFPETSHLMKMLRNVGTERPRWPNEEEPFMGMHQMRPTLSSSHNVQPTNASKWERAISVNVGPVLQYPARAAQVVPYSYQPVTNSNRFKDSNAGSPLMSQSAADEGSRTGIKGSGILSSVAEPSKLKSVIGNTEPGSSTPQPHQGSASVSRQMTIFNVGQAHVFDDVHPNKAEVIMTLAGSNGGSWSTSFLPNSSIKPSGGEPVSLSGGQGNMSMKDARVSGADEKSEDKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GQT3</accession>
    <name>A0AAD5GQT3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Rapid ALkalinization Factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_002629</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the plant rapid alkalinization factor (RALF) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005794">
        <property type="protein sequence ID" value="KAI7751902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GQT3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009506">
        <property type="term" value="C:plasmodesma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008801">
        <property type="entry name" value="RALF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33136:SF107">
        <property type="entry name" value="RAPID ALKALINIZATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33136">
        <property type="entry name" value="RAPID ALKALINIZATION FACTOR-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05498">
        <property type="entry name" value="RALF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0372">Hormone</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042191180" description="Rapid ALkalinization Factor" evidence="7">
        <location>
            <begin position="31"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7751902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="121" mass="13267" checksum="CD513D3C6E4D79BA" modified="2024-05-29" version="1">MPISNNIILLLHLLITVFMLSTVIMSPSAASNGGEDQFSWNMSKQGGCRGSIAECMADGEIEMESERTRRILATSNYISYDALQGNNVPCSQRGASYYNCQSGGQANPYQRGCSTITRCRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GRC6</accession>
    <name>A0AAD5GRC6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">TNase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_012803</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005711">
        <property type="protein sequence ID" value="KAI7752290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GRC6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035437">
        <property type="entry name" value="SNase_OB-fold_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016071">
        <property type="entry name" value="Staphylococal_nuclease_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002071">
        <property type="entry name" value="Thermonucl_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12302">
        <property type="entry name" value="EBNA2 BINDING PROTEIN P100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12302:SF3">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00565">
        <property type="entry name" value="SNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00318">
        <property type="entry name" value="SNc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50199">
        <property type="entry name" value="Staphylococcal nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01284">
        <property type="entry name" value="TNASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50830">
        <property type="entry name" value="TNASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TNase-like" evidence="4">
        <location>
            <begin position="103"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7752290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="350" mass="39531" checksum="E7DDADE5C5182829" modified="2024-05-29" version="1">MAGIIPSHHLPPPQAVAVMTDYLTTTTAFPPALINTSVIPFISPLRYRQEEPSTLVVHTEPDLDRLLSCLPHLLVELRAGEAPSLTDGLKFELCTLPVDAKAVADGDTVTVYVSTLDGWELSRVPREVEVAAVERSEALAERNYSKADALQKQITDAGYQLMRIDNQEILVRKYRIRLRGIDAPERSMPYGKEAKDELVKIIEGKCLKILVFGNDRYGRFVGDVYCNGIFVQESLLKKGLAWHYTDYDKRPELEKVIDYRSLDCANDNHLFFCFVRWLNWFDISGRKMRGLRESGCGLRQNLKRRGNGGRVVVKTDIHACMPSWQSGSTFVDDMAPSRFVIVHVVATFKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-10-08" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GUN8</accession>
    <name>A0AAD5GUN8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">S-adenosyl-L-methionine-dependent methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_023568</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005175">
        <property type="protein sequence ID" value="KAI7753894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007213">
        <property type="entry name" value="Ppm1/Ppm2/Tcmp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43619">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE-DEPENDENT METHYLTRANSFERASE YKTD-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43619:SF2">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE-DEPENDENT METHYLTRANSFERASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04072">
        <property type="entry name" value="LCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7753894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="299" mass="34115" checksum="71178119EB0948BF" modified="2024-05-29" version="1" fragment="single">MAIQSFLVRPPAIPVITQPLSPVSNNRNYRYKITAKTNSHQDSNHDHPLFQSAITRASYRFQESLRPEPLFVDPYAGCFAPPNLDLDEKLDQKLHCYCIGTRFIDDQLLSVTKGDDGAKQVVLFTDGMDTRAYRLKWPSSTVIYHVSPQSVFNAASQKLQDVGAKIPRSCLHLHVPLESSDMQQLLCDKGFSGGRPSIWVFQGFPVTNLAAFKEILFMVSCLAMKGCLFLGELPVWLTETQVGIKSSVEKWMDEIFMSYGFRMEIWRREFRRVEEDGDEDGFEXXXXMFATAIDEIYHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GV43</accession>
    <name>A0AAD5GV43_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_013473</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000387">
        <property type="protein sequence ID" value="KAI7756445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GV43"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35702">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35702:SF1">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7756445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="262" mass="28676" checksum="9BECE82D881D7EE4" modified="2024-05-29" version="1" fragment="single">MMMDFQKKRFQLLGFIIGLIILSFTAEKFREIVGERGSSQSGKFTIFNCFDGGSGTVACLVKEGVKLYTYNIRTMHVEVARNKAIEASLADAISQGMEPTAAAKKAQQDGAKAAKLATRKAKRIIGPIISSGWDFFEALYYGGTITEGYLGEERFGRFGYLIGSQLGSWVGGRMGLMVYDIANGVHFLLEFGQPEENVGESVDFSQDGQTEETVGEQTAYGASFLETIMGYVSSYLAETSKDISSETVEEFMKATSLMKNSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GV99</accession>
    <name>A0AAD5GV99_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">M8C21_016783</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">AA19_3_7</strain>
            <tissue evidence="17">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="JAMZMK010005560">
        <property type="protein sequence ID" value="KAI7753028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048544">
        <property type="term" value="P:recognition of pollen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00028">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd01098">
        <property type="entry name" value="PAN_AP_plant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd14066">
        <property type="entry name" value="STKc_IRAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000060">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.90.10.10:FF:000005">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.90.10.10:FF:000029">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000195">
        <property type="entry name" value="G-type lectin S-receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.90.10.10">
        <property type="entry name" value="Bulb-type lectin domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001480">
        <property type="entry name" value="Bulb-type_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036426">
        <property type="entry name" value="Bulb-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000858">
        <property type="entry name" value="S_locus_glycoprot_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32444">
        <property type="entry name" value="BULB-TYPE LECTIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32444:SF241">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01453">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08276">
        <property type="entry name" value="PAN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00954">
        <property type="entry name" value="S_locus_glycop"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00108">
        <property type="entry name" value="B_lectin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00473">
        <property type="entry name" value="PAN_AP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51110">
        <property type="entry name" value="alpha-D-mannose-specific plant lectins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50927">
        <property type="entry name" value="BULB_LECTIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50948">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="462"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="599"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1178"/>
            <end position="1198"/>
        </location>
    </feature>
    <feature type="domain" description="Bulb-type lectin" evidence="15">
        <location>
            <begin position="44"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="16">
        <location>
            <begin position="355"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="519"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="domain" description="Bulb-type lectin" evidence="15">
        <location>
            <begin position="765"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="16">
        <location>
            <begin position="1071"/>
            <end position="1163"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="1235"/>
            <end position="1519"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="547"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="1262"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="KAI7753028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="1552" mass="174365" checksum="46DEE0312DA8131E" modified="2024-05-29" version="1">MFTLLSVKARLDSMECLNMILVLFYCCSLLFILITDSASAAVATDTIFPNQTLXXXTIVSAGEIFELGFFSTENSSNRYLGIWYSLKRVSPRTVVWVANREVRLLDNSSTLNLDNEGNLRLLSVSNTLAWSSQSSKQVTNGSPVAQLLDTGNLVIRDDISNDLIWQSFDHPGDTWLPGMKIGVDRVTGISWHLASWKTSDDPSVGSYAVWLNTNGYPQLFNLKDQSSLQQRIGYWNGLGFTGIVSVSALRQENLYTYEFVLNDEEIYTRFVLRNDTILTRMTLDPVGNFVQLIWIDRTQDWVVYLSQREDGCDQYSFCGPYGICSINNSPPCACFQGFEPKLPEEWSVAKWSNGCKRQIYLTPEKGHNFIKFSNLKLPDSRDSWFNRSQSLEECKNLCANNVSCTAFANSDITKGGSGCLMWFGELMDIRAAPEEDISGQDIYIKMVKPKLTLDKKKSNLKVILPIMSVIVMLSFALILYFWRRKQKSNNKGLVRKDQKQDSDLPFFSLNEVAKATSNFSINNKLGQGGFGAVYKGVLEGGVEIAVKRLSKTSKQGIVEFQNEVICIAKLQHRNLVKLLGCCIQGEEMMLIYEYMPNKSLDFFLFDVFSFGVLVLEIVSGKKNRGFSHQDHCDNLLGHAWRLYTEAKPLELVDVTLGDSWTASEVLQSIHVGLSCVQQHADDRPSMWSVVHMLGGEGALPPPKQPGFFTETIMEIVILARSVDHELASQLSTVKPSPDSMERLDMILFCGGLLFILITDSTTAATDTISPNQTLKNGNTIVSASEIFELGFFSFENSSFQFLGIWYKTISPRTYVWVANREVPLLDDSSILKLNDEGNLQLLSVSNTQVWTSQSSKLVTNSTPVAQLLDSGNLVIRDDISSDLIWQSFDHPGDTWLPGMKIGVDLVTGRNWNLTSWKTSDDPSLGSYTVWMNTNGYPQLFNMKHESSLQQRIGYWNGLGFSGALGLRSNSYTFDFVLNEKEIYSRFALVNSTSIIRMKLNPEGNFVLYVWSDRRDTWQLYLAPSVDACDRYGFCGPYGICSINNAPPCSCFEGFEPKLPEEWSVADWSNGCKRQISLTLEKGHNFTKFSNLKLPDSQSSWFNRSMSLVECENFCSSSLSCTAFASTDIRGRKSGCLTWSSELIDIREAQQGDVSGQEIYIKMINPELIRYKKKSPLKVILPILSAVVMLIFALILYSWRRKKELNNKALLNKKQKQDSDLPFFSLNEVVMATSNFSVNNKLGQGGFGSVYKGLLEGGEIAVKRLSKTSKQGIVEFQNEVICIAKLQHRNLVKLLGCCIQGEEMMLIYEYMPNKSLDFFLFDKNNSLLLDWPQRYHIINGIARGLVYLHQDSRLRIIHRDLKASNILLDSNMNPKISDFGLARMFKEYETEANTNHVVGTLGYISPEYAANGLFSLKSDVFSFGVLVLEIVSGKKNRGFSHQDHHDNLLGHAWRLYTEGNPLELIDVALGDSWTATEVLQSIHVGLSCVQQHADDRPSMSSVVHMLGGEGALPPPKQPGFFTETSKPEVEATIIMSKVPISNNKVTITQPEAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GW80</accession>
    <name>A0AAD5GW80_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pesticidal crystal cry8Ba protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_028896</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010003076">
        <property type="protein sequence ID" value="KAI7754526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GW80"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31110">
        <property type="entry name" value="PESTICIDAL CRYSTAL CRY8BA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31110:SF2">
        <property type="entry name" value="PESTICIDAL CRYSTAL CRY8BA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="173"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="192"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="203"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="234"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="248"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="273"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7754526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1091" mass="122343" checksum="98C3A2B566C75845" modified="2024-05-29" version="1" fragment="single">HDSSSELSLERENNQKVREREKNKQKGLDTNALRWVKEGSNNPKKEVPYYVPNQRSQIDPVSSRNGNRGFGLPPASKFRSGHLPGVIPMSQVLPGEDTTSASDNDMSTDSEEEVYVGRYSLDSSPQEDIVFQSNRSYDPSRVRPRYHVYSSDVSSSVETMGARRINKNRVRNSSICVEDESDDSNGSSEFSTTQVPSQVNGDASRKDHKAAFDEDIPSAPPFLGSDVEIKQQEHLQPSKSNHTPLRANSHDFEVKPAIERKTPSVDLKDDQNETSTSSNRNARAKSGPSSGSFHARLPTFHASSLGPWHSVIAYDACVRLCLHAWAKGCMEAPMFLENECALLRSTFDLQQVLLQSEGELLVKRSSEAGSKGGAAKPKKMVGKMKVQVRKVRMALDPPTGCNFSSFKPPKVKLETVKRHCSTVQSKLSSGWQAVKKIPFSPREPIGGSISQQSLAYMQASTQYIKQVSSLLKTGVSSLRSTTSANEVLQETYSCLLRLKSSAEEDTVRMQPGSSEVHIFLPDSLGDDLIVEVYDSKGNHYGRVLVQLATISEDPNEKLRWWSIYREPEHDLVGKVQLHINYTTSLDDNIKCGSVAETVAYDIVLEVAMKVQNFQQRSLLLHGPWKWLLIEFASYYGVSDAYTKLRYLSYIMDVATPTADCLSLVYDLLLPVVMKGHSKSTLSHQENRILGEIEDQIEQILALVFENYKSLDEQSPSGIMDTFRPATGVAAPVLEHAVKLYKLTHDILSPEAQNKLYSYFQVAAKKRCRRHLTETDEFVSGNGEGILMDCVAVSTAYQKMKHVCLNIRNEIFTDIEIHNCNILPSFIDLPNLSSAIYSAELSSRLRAFLVACPPTAPSPHVTDLVIATADFQKDLASWNINPVKSGVDAKELFHLYIMIWIQDKRLSLLETCKLDKAKWSGVRTQHSTTPFVDEMYDRFKETLNDYEVIICRWPEYSFTLENAVADIEKAVIEALDKQYADVVAPLKENMTPKKFGLKYIQKLTKRATDPYVVPHELGILLNSMKRMLDVLRPKIELQLKSWGSCCIPDGGNVAPGERLSEVTVMLRSKFRNYLQAVVEKLVDNVSIFFSIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BLK5</accession>
    <name>A0AAD5BLK5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_019261</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011897">
        <property type="protein sequence ID" value="KAI7725585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BLK5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7725585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="84" mass="9516" checksum="0B0D3A8D7F0785BC" modified="2024-05-29" version="1">MKLNKNSLKRLRDRLLKVVAVGVMLVVLCEISFYLGGRYCSVKYEVATEESTSVQVKFVAFPQCSADLQDYTPCTDPKVYCVTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BP04</accession>
    <name>A0AAD5BP04_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">LIM zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_021699</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011522">
        <property type="protein sequence ID" value="KAI7726845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BP04"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08368">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09396">
        <property type="entry name" value="LIM_DA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045218">
        <property type="entry name" value="DA1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022087">
        <property type="entry name" value="DA1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24209:SF25">
        <property type="entry name" value="PROTEIN DA1-RELATED 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24209">
        <property type="entry name" value="PROTEIN DA1-RELATED 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12315">
        <property type="entry name" value="DA1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0440">LIM domain</keyword>
    <keyword evidence="1 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="4">
        <location>
            <begin position="94"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7726845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="442" mass="50627" checksum="1952352AD1081E82" modified="2024-05-29" version="1" fragment="single">KSFIRQWIGLAGFSKGRTDARSEFEDFDRAIAMSLAEGRRRHGFNDDFLRKEDERLAKALQGRLKLEPSPWNRNGNRNANVRQPMSFPHSTGLGTCAGCNNVISHGQFLRCMGAVWHPECLKCHGCKLPISDYEFLLSGNYLYHKSCFRDNHHPRCDVCRHFIRTDAAGFVEYKVHPFWAQKYCPHHERDGTPRCCSCDRFEPKEVQYAALNDGRKLCLECLDSAIMGTADCQPLYLDIQKFFASLNMKLEQKIPLLLVERQALNEAMDGETNGHHHMPETRGLCLSEEQIVSTVSRQPRIGMGNRVPSMRIEPYQLTRQAEVTAILILYGLPRLLTGSILAHEMMHAWLRLKGYPTLRPDVEEGICQTVAHMWLTSEIASLSRQGRRFPFEKKLADFFKQQIESDTSPVYGNGFRAGNRAVLKYGLQQTLYHMRSTGRFPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BPX6</accession>
    <name>A0AAD5BPX6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FBD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_014349</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011719">
        <property type="protein sequence ID" value="KAI7726293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BPX6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006566">
        <property type="entry name" value="FBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055411">
        <property type="entry name" value="LRR_FXL15/At3g58940/PEG3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31639:SF326">
        <property type="entry name" value="F-BOX DOMAIN, FBD DOMAIN, F-BOX-LIKE DOMAIN SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31639">
        <property type="entry name" value="F-BOX PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08387">
        <property type="entry name" value="FBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24758">
        <property type="entry name" value="LRR_At5g56370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00579">
        <property type="entry name" value="FBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FBD" evidence="1">
        <location>
            <begin position="182"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7726293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="261" mass="30173" checksum="AB93E309C9748A0B" modified="2024-05-29" version="1">MLWLSDEYKLPPAFFNLQQLTVLKLHNCAFRPPETFSGFSRLVKLLFNKVSITAKDFLWLISNCPRLEALALIGDENHLLGCWNSNFVELFERLPLLKHLLMSAYPIKCFATGFMPKKLPTSLVHLKYLHLHRLRFGREVDFLCALSLVASFNVEITEIEMEDNPTEAMSQIAMNLIDHYNSFVILDHLRVIRIRNFTNTKIGMDFVKLILAKSPMLKKVDIYINHQVDINGELKIVKELIRYPRASAKAEIMIKKPTLSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BPZ5</accession>
    <name>A0AAD5BPZ5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_009518</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011386">
        <property type="protein sequence ID" value="KAI7727412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BPZ5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34564">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34564:SF3">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042113648" evidence="2">
        <location>
            <begin position="20"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="56"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7727412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="105" mass="11995" checksum="8E271EE7B19F1508" modified="2024-05-29" version="1">MSRPLLLVSLLLLMVFTSQFEWNQPLVNEVEARPLVLSQKQQYLLEKEESVKEKIILSQEKQIQKLKTLVQSLEEQLLACKGKDDFVNDTAGVSRGTLTCNKHVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BQ43</accession>
    <name>A0AAD5BQ43_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_022509</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011410">
        <property type="protein sequence ID" value="KAI7727214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BQ43"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.50:FF:000022">
        <property type="entry name" value="F-box protein FBW2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38926">
        <property type="entry name" value="F-BOX DOMAIN CONTAINING PROTEIN, EXPRESSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38926:SF81">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="33"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7727214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="279" mass="31795" checksum="D56B7670D30BF4BD" modified="2024-05-29" version="1">MSKKTLMLSPTDSNTTVVVAVTQHWRRWEELNPEILALIFKKILPVEEMVRSVPLVCKGWKEVVCGPYCWSEIDLEGWCRRRNDGHAVDMVVKKVVRRSKFMVQRMSVYGMRETGGNFLKVLQMPMTDITDQMVTKHIKPLPNLTVLDISHCVKITSKGLATFGHQCKSLLHLKRNMPAFEDQLPVDDSETKAIAETMPHLQQIELCFGRFGDSGVSEILSKCKALTHLVIQGSWNVELNGDLAEICERLEHFHSPWIDDDDRFSDMSECGDADFTDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BR09</accession>
    <name>A0AAD5BR09_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidase C14 caspase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_005860</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C14B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011303">
        <property type="protein sequence ID" value="KAI7727875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BR09"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12660">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050452">
        <property type="entry name" value="Metacaspase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011600">
        <property type="entry name" value="Pept_C14_caspase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48104:SF32">
        <property type="entry name" value="METACASPASE-1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48104">
        <property type="entry name" value="METACASPASE-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00656">
        <property type="entry name" value="Peptidase_C14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase C14 caspase" evidence="2">
        <location>
            <begin position="5"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7727875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="259" mass="28563" checksum="E877B1BCB527FD94" modified="2024-05-29" version="1">MKYLLINKFKFPESSILMLTEEETDRYRIPTKHNIRMAMYWLVQGCQPGDSLVFHFSGHGSQQRNYTGDEVDGYDETLCPLDFETQGMIVDDEINATMVKPIPHGVKLHAIIDACHSGTMLDLPFLCRMDRSGRYVWEDHSPQSGIFKGTNGGEVISFSGCDDDQSSADTASLSKVTSTGAMTFSFIQAIERGHATTYGDMLTSMRTTIRKNESDVGGGAVTSLLGMLLTGGSLGGGLRQEPQLTASEPFDVYTKRFTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BRS9</accession>
    <name>A0AAD5BRS9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BAR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_026944</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011404">
        <property type="protein sequence ID" value="KAI7727283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BRS9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07307">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037488">
        <property type="entry name" value="At2g33490-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34119">
        <property type="entry name" value="HYDROXYPROLINE-RICH GLYCOPROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34119:SF1">
        <property type="entry name" value="OS04G0394700 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="243"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="333"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="460"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="254"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="340"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="374"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7727283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="483" mass="54091" checksum="DA4F6004E6914409" modified="2024-05-29" version="1">MKSPFKKLRGFGLGSKTKKQQEAELDEVSQASKDMQHIKDCYDTLLSSAAATANSTYEFSESLGEIGDCLLQKTSLNDDTGKAVQMLGKGQFQIQKLLDNYRSHILRTITIPSESLLNELLVVEEMKKQCEVKRMVYDEMKHKHKERRRLGLGRNKGEYVSSKQVQAAKEEFEEDATLFVFRIKSLKGGQSRSLLTQAARHHAAQVTSFFMSFFRKALKSLETIDPYIQVVTKQHHIDYQFSGLEDDDRDSRNDPMNQVSSSENSMELADLTFPQVNSVKEQRKPLWNSSTYNIAVNTSSKSAPLSVQSNIEPREKFRQSSVRKLNTYVLPTPLDGNPRFTDTPQPASTRCSDRNIWHSSPLKTNKFEEKVKFSPSNKNSKPTQLPSPLQYQYPFGDNNVKTYSFSVPIAATPHSSSSLLNSGKLVSGNFVSSPKINELHELPRPPANLVSPSKGGGFSAPLVTFKDSNKSPDAASVTESSRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2025-10-08" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BSE1</accession>
    <name>A0AAD5BSE1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_027657</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the 19S regulatory particle (RP/PA700) base subcomplex of the 26S proteasome. The 26S proteasome is composed of a core protease (CP), known as the 20S proteasome, capped at one or both ends by the 19S regulatory particle (RP/PA700). The RP/PA700 complex is composed of at least 17 different subunits in two subcomplexes, the base and the lid, which form the portions proximal and distal to the 20S proteolytic core, respectively.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011214">
        <property type="protein sequence ID" value="KAI7728444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000502">
        <property type="term" value="C:proteasome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000008">
        <property type="entry name" value="26S protease regulatory subunit 10B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000027">
        <property type="entry name" value="26S protease regulatory subunit 10B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000034">
        <property type="entry name" value="26S protease regulatory subunit 10B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050221">
        <property type="entry name" value="26S_Proteasome_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032501">
        <property type="entry name" value="Prot_ATP_ID_OB_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23073">
        <property type="entry name" value="26S PROTEASOME REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16450">
        <property type="entry name" value="Prot_ATP_ID_OB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0647">Proteasome</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="12">
        <location>
            <begin position="172"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00061931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7728444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="391" mass="43904" checksum="134F43B88F187CC7" modified="2024-05-29" version="1">MSTDGEDAVRRRAAVTDYRKKMLQHKELDSRVRTVRETLRSTKKDYAKTEDDLKSLQSVGQIIGEVLRPLDNERLIVKASSGPRYVVGCRSKVDKEKLTAGTRVVLDMTTLTIMRALPREVDPVVYNMLHEDPGNVSYSAVGGLSDQIRELRESIELPLMNPELFLRVGIKPPKGVLLYGPPGTGKTLLARAIASNIDANFLKVVSSAIIDKYIGESARLIREMFSYARDHQPCIIFMDEIDXXXXADREIQRTLMELLNQLDGFDQLGKVKMIMATNRPDVLDPALLRPGRLDRKIEIPLPNEQSRMEILKIHAAGIAKHGEIDYEAVVKLAEGFNGADLRNVCTEAGMSAIRAERDYVIHEDFMKAVRKLNEAKKLESSAHYNADFGKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BTD0</accession>
    <name>A0AAD5BTD0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_002196</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011029">
        <property type="protein sequence ID" value="KAI7729257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BTD0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7729257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="52" mass="5722" checksum="AB1FD4799BFB3AA7" modified="2024-05-29" version="1">MGGVCGRVTVVLRWRHKVVLGVMSSGVGCGRRLCWVWRMVDGQLDEEDDGGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BTS8</accession>
    <name>A0AAD5BTS8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cyanobacterial aminoacyl-tRNA synthetase CAAD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_019667</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011056">
        <property type="protein sequence ID" value="KAI7729164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BTS8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009535">
        <property type="term" value="C:chloroplast thylakoid membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025564">
        <property type="entry name" value="CAAD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033344">
        <property type="entry name" value="CURT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33222">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33222:SF9">
        <property type="entry name" value="PROTEIN CURVATURE THYLAKOID 1B, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14159">
        <property type="entry name" value="CAAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="33"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="61"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="Cyanobacterial aminoacyl-tRNA synthetase CAAD" evidence="3">
        <location>
            <begin position="22"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7729164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="94" mass="10030" checksum="D6C9576C06404C4B" modified="2024-05-29" version="1">MATGEAAPEAVSTDVQELLKPIQEAWDKVEDKYAVSSLAVAGGVFLWGSTGMISAIDRLPLVPGVLELVGIGYTGWFAYKNLVFKPDSQPEASM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BUL6</accession>
    <name>A0AAD5BUL6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_001595</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010964">
        <property type="protein sequence ID" value="KAI7729614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BUL6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045927">
        <property type="term" value="P:positive regulation of growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045021">
        <property type="entry name" value="PSI1/2/3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31730">
        <property type="entry name" value="OS01G0873900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31730:SF32">
        <property type="entry name" value="PROTEIN PSK SIMULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="144"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="153"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7729614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="193" mass="21976" checksum="2D38A06D06DCCFA1" modified="2024-05-29" version="1">MSNTKLGQYRAHHGFGWVGEWANTGSEANEKLSCTVDSLRIETLYHADKEKTEECILKLVIWLHHLVCQSKAINSGIKSPVKSPIRSPNRKRIQLTANRPDSPLPALTTEDQEMLQDVCKRKPTPVISRSQEFVARNRLSEQYRLTKSSSHSPTREIKRDPFPIKRPSSVPIINFDIDRMTAMDVIDDIKSVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BUY9</accession>
    <name>A0AAD5BUY9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_004411</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010841">
        <property type="protein sequence ID" value="KAI7730111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BUY9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7730111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="142" mass="15910" checksum="735CBFF823B20253" modified="2024-05-29" version="1" fragment="single">SNGELISRQINRGAFDLLCNRNNSQPAMLFFVYVLNRIYSVMPPRKRKSTSNNDCDQASSNDGATRGCQTAGQAMKIEETSKSSKKIALESSRPFRKKKATVSTTNFFYDQDKPKKVSSDADNSVVAATRDRKIPSYYEHGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BVF2</accession>
    <name>A0AAD5BVF2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_025862</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010011047">
        <property type="protein sequence ID" value="KAI7729188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BVF2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd14016">
        <property type="entry name" value="STKc_CK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000222">
        <property type="entry name" value="casein kinase 1-like protein HD16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055900">
        <property type="entry name" value="DUF7477"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24289">
        <property type="entry name" value="DUF7477"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="129"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7729188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="695" mass="78246" checksum="0D14770F326CF58E" modified="2024-05-29" version="1">MPELRSGVRHPVVENRSEGNYIKTRAAKAKHAAAAVLQLNPQPQPQQQTKPKRHKNNNNKDIIKGLVENPVIIITSDPEQDNNNNNNNINNNNNKVAEIVGMSANKEEEEEGNTTPFPDMVQVGGSPLYKVERKLGKGGFGQVFVGRRVSGGTERISGPGATEVAIKFEHRNSKGCSYGPPYEWQVYNTLGGSYGVPRVHYKGKQGDYYVMVMDMLGPSLWDVWNSSGQTMSSEMVACIAVESLCILEKMHFRGYVHGDVKPENFLLGQPSTPQEKKLFLVDLGLATKWRVTAGGQHVEYDQRPDIFRGTVRYASVHAHLGRTASRRDDLESLAYTLVFLLRGRLPWQGYQGDNKSFLVCKKKMTTSTEALCEYCPAPLKQFLDIIVSMKFDEEPNYSKLISLFEDLLSPNPVIRPINTDGAQKIILQVGQKRGRLNLDEEDDGQPRKKTRTGVHATQWISVYNARQPMKQRYHYNVTDERLAQHIERGNADGLLISSVASSANLWALIMDAGTGYSSQVYELSPVFLHKEWIMEQWDENYYISAIAGASNGSSLVVMSRGTKYSQQSYKVSDSFPFKWINKKWSEGFHVTSMATAGTRWGVVMSRNSGFREQVVELDFLYPSEGIHRRWNSGYRITATAATMDQSALILSMPQRKPNDETQETLRTSQFPSTHVKDKWAKNLYLACICYGRTVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BWA9</accession>
    <name>A0AAD5BWA9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PGG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_026167</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010618">
        <property type="protein sequence ID" value="KAI7730832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BWA9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026961">
        <property type="entry name" value="PGG_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24177">
        <property type="entry name" value="CASKIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24177:SF467">
        <property type="entry name" value="PGG DOMAIN, RETROTRANSPOSON COPIA-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13962">
        <property type="entry name" value="PGG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="190"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="228"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="269"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="301"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="PGG" evidence="2">
        <location>
            <begin position="180"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7730832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="348" mass="39684" checksum="C88859E1EAED0E49" modified="2024-05-29" version="1" fragment="single">PHIKHLREDKVKHNLALEILKFICFEVSKLKSNYYEHYGEAIIFAVENNTPEVIENIIKLFPDSVWMGNNGYLVPQLSIMNRCENVYNFLVHEVTQDKFLHKTISDKDKNNLLHLVGQLAPMDKLNMVTGAALQMQRELQWFQEVRKFIRPIDIEARNIKAETPIMVFRKEHKELRKEGEEWMKKTADSYTITAALIITIVFAAAITVPGGNNGDTGKAIYETRPSFIIFAISDAISLFTSTTSLLLFLSILTARYAEEDFLYKLPKRLIFGLVMLFMSVTTMMIAFSATLYIMFGHGKSWIVIPIGAMTCLPIASFVTLQLPLLVDLISSTYGHGIFGKQSKFWKTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BXN7</accession>
    <name>A0AAD5BXN7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NADPH-dependent pterin aldehyde reductase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_025556</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010462">
        <property type="protein sequence ID" value="KAI7731362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BXN7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006760">
        <property type="term" value="P:folic acid-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000434">
        <property type="entry name" value="NADPH-dependent pterin aldehyde reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053241">
        <property type="entry name" value="NADPH_pterin_aldehyde_rdct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45267">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45267:SF2">
        <property type="entry name" value="NADPH-DEPENDENT PTERIN ALDEHYDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7731362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="245" mass="25910" checksum="57E5BCF97D4D3DD6" modified="2024-05-29" version="1">MTTMNAVMNGGSGGGQKTVLITGVSRGLGKALALEIANRGHSIIGCSRSQDKLKSLQSQLSSSSSSDDNDNHKHLFLDLDVCSNSSVQELARVVMEKKGVPDIIGTINKNNRLWEVPEEEFNSVLDTNVKGIANMLRHFIPLMIEKKQGIIVNMSSGWGRSAAAQVAPYCASKWAVEGLTKSVAKELPSGMAAVALNPGVINTDMLASCFGASASLYQAPDSWAPKAADMILNLTMADNGSSLSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BXZ4</accession>
    <name>A0AAD5BXZ4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_023932</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010380">
        <property type="protein sequence ID" value="KAI7731808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BXZ4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="32"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="4"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7731808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="127" mass="14384" checksum="3DF78BD62376F60D" modified="2024-05-29" version="1">MEYEKARIERIKQLKAKMKELRLRLTKTSFKGTIRRKDKNNTNLEKDAENMASTGNKKPSSDPIDLLSSHKVETISVHRLCATLCTILSPTNLVTNVELSEGPKKEIVDGSVVNTHLLRLRKKSVKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BY00</accession>
    <name>A0AAD5BY00_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">TF-B3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_022298</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010433">
        <property type="protein sequence ID" value="KAI7731542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BY00"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.330.10">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015300">
        <property type="entry name" value="DNA-bd_pseudobarrel_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101936">
        <property type="entry name" value="DNA-binding pseudobarrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7731542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="57" mass="6723" checksum="378B7D0352AE744A" modified="2024-05-29" version="1" fragment="single">MVSSFFKPIVFNHATFQAAEIIEDVKDSQSYVITNGWNKFVEENDFIIFELLKDRCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BYB1</accession>
    <name>A0AAD5BYB1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Beta-galactosidase</fullName>
            <ecNumber evidence="3 7">3.2.1.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_023048</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.</text>
            <dbReference type="EC" id="3.2.1.23"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the glycosyl hydrolase 35 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.23" evidence="3 7"/>
    <dbReference type="EMBL" id="JAMZMK010010682">
        <property type="protein sequence ID" value="KAI7730684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BYB1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004565">
        <property type="term" value="F:beta-galactosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22842">
        <property type="entry name" value="Gal_Rha_Lectin_BGal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000076">
        <property type="entry name" value="Beta-galactosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000006">
        <property type="entry name" value="Beta-galactosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048913">
        <property type="entry name" value="BetaGal_gal-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041392">
        <property type="entry name" value="GHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031330">
        <property type="entry name" value="Gly_Hdrlase_35_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019801">
        <property type="entry name" value="Glyco_hydro_35_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001944">
        <property type="entry name" value="Glycoside_Hdrlase_35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000922">
        <property type="entry name" value="Lectin_gal-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043159">
        <property type="entry name" value="Lectin_gal-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23421">
        <property type="entry name" value="BETA-GALACTOSIDASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21467">
        <property type="entry name" value="BetaGal_gal-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17834">
        <property type="entry name" value="GHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01301">
        <property type="entry name" value="Glyco_hydro_35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02140">
        <property type="entry name" value="SUEL_Lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00742">
        <property type="entry name" value="GLHYDRLASE35"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01182">
        <property type="entry name" value="GLYCOSYL_HYDROL_F35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50228">
        <property type="entry name" value="SUEL_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042085304" description="Beta-galactosidase" evidence="9">
        <location>
            <begin position="19"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="domain" description="SUEL-type lectin" evidence="10">
        <location>
            <begin position="750"/>
            <end position="836"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7730684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="836" mass="92308" checksum="19EE3E094FFF4EF3" modified="2024-05-29" version="1">MMRLLIVWFLVVVAKVTAVNVTYDHRALIIDGRPRVLVSGSIHYPRSTPDMWPGLIQKSKEGGLDVIETYIFWNLHEPVRNQNLKTPIARSFKRNEDKYDFEGRKDLIKFVKLVANAGLYVHLRIGPYISAEWNYGGFPLWLHFIPGIVFRTDNEPFKAEMKRFVAKIVGMMKEENLYASQGGPIILSQIENEYGNIDSPYGPAAKSYIKWAANMATSLDTGVPWVMCQQRDAPDPIIDTCNAFYCDGFKPNAENKPTMWTENWTGWFLLFGEAAPYRPVEDIAFAVTRFYQRGGTFQNYYMYHGGTNFGRTTGGPFIATSYDYNAPLDEYGMANNQLVFVYLITYGSSDPFHTSCNKSVYIMQGHRDNLNGDANVNFNGNSYHLPAWSVSILSDCKNLVFNTAKINSMATIRRFVATSVGHDLSDSMAISSKWSYVSEPVGISSNDAFNRPGLVEQINTTADQSDYLWYSISIDVKGAEPFLHGGFQTTLHVKSLGHVLHLFVNGQLEGSANGNMFTHGFTKDFSVVLKPGKNKLDLLSLTVGLQHFGAFFDKAGAGVTGPVKLEGLTNGSTVDLSSQRWTYQIGLKGESLGLNTGGSSLWVSGSPKSQPLTWYKTNFDAPAGDNPIAIDFTGMGKGEAWVNGQSIGRYWPSYIAPTGGCSVCSYKGPYNFTKCLKNCGKPSQNLYHVPRSWLKPSANVLVMFEEIGGDPTQISFKTQELESLCSQVSESHPLPMEAWSQDDPTRKSNPMAKPRVLLECPDPNQVISSIKFASFGTPQGKCGSFSHGYCKSSNALSITQKACIGSRTCNIEVSSSTFGDPCVGVVKSLAVEASCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5BYC9</accession>
    <name>A0AAD5BYC9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">M8C21_023035</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">AA19_3_7</strain>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010682">
        <property type="protein sequence ID" value="KAI7730671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5BYC9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000044">
        <property type="entry name" value="Putative LRR receptor-like serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052059">
        <property type="entry name" value="CR_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47973">
        <property type="entry name" value="CYSTEINE-RICH RECEPTOR-LIKE PROTEIN KINASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Protein kinase" evidence="15">
        <location>
            <begin position="1"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAI7730671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="265" mass="29724" checksum="CD6B81AA2805D6F6" modified="2024-05-29" version="1" fragment="single">GILNDGSVVAVKQLSVASHHGRSQFITEISTISTVQHWNLVKLHGSCVEGSRRLLVYEYLENKSLDQALFGKNKLCLDWSTRFNICLGTARGLAYLHEESRPRIVHRDVKSSNILLDGELCPKISDFGLAKLYDDKRTHMSTRVAGTIGYLAPEYAMRGHLTSKADVFGFGIVCLEILSGRPNYDEKLDPERKYLLQWGWSLYESNRHLELIDPSLSSFDEQEATRMIGVALLCVQASPTLRPAMSRVITMLSGDVEINRVTTKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C029</accession>
    <name>A0AAD5C029_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Delta(24)-sterol reductase</fullName>
            <ecNumber evidence="2">1.3.1.72</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_015364</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.72" evidence="2"/>
    <dbReference type="EMBL" id="JAMZMK010010124">
        <property type="protein sequence ID" value="KAI7732886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C029"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050614">
        <property type="term" value="F:Delta24-sterol reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008202">
        <property type="term" value="P:steroid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.465.10:FF:000012">
        <property type="entry name" value="delta(24)-sterol reductase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040165">
        <property type="entry name" value="Diminuto-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10801">
        <property type="entry name" value="24-DEHYDROCHOLESTEROL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10801:SF20">
        <property type="entry name" value="DELTA(24)-STEROL REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="8">
        <location>
            <begin position="46"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="538"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="543"/>
            <end position="554"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7732886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="566" mass="66460" checksum="BD0C3782EF424BE4" modified="2024-05-29" version="1">MADLEAPLVRPKRKKVWVDYFVQFRWILVIFVVLPISFTLYFLTYLGDVRSQWKSYEQRQKEHEENVKKVQKRLKERNPSKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILEIDQERMIAKCEPLVNMGQITRATVPMNLALAVVAELDDLTVGGLINGYGIEGSSHLYGLFSDTVVAYEIVLADGRVVRATKDNEYSDLFYAIPWSQGTLGLLMSAEIKLIPIKEYMKLTYKPVRGNVRDLAQAYIDSFAPDFDDENNEKVPDFVETMIYNPHEGVCMTGKYASKEEAKMKGNKINSVGWWFKPWFYQHAETALKKGEFEEYIPTREYYHRHTRCLYWEGKLILPFGDQWWFRFLFGWLMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWVDREMEIYPLWLCPHRLYKLPCKTMIYPEEGFEQERRQGDTSYSQMYTDVGVYYTPGPVFRGEEFDGVDAVRRLETWLIENHGFQPQYAVSELDEKKFWRMFDAGLYEKCRNKYGAVGTFMSVYYKSKKGRKTEKEVQEAEKAQVETPYAEADQPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C086</accession>
    <name>A0AAD5C086_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pollen Ole e 1 allergen and extensin family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_025237</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010399">
        <property type="protein sequence ID" value="KAI7731704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C086"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042026980" description="Pollen Ole e 1 allergen and extensin family protein" evidence="1">
        <location>
            <begin position="26"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7731704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="174" mass="18754" checksum="3C8E7C2867B323E3" modified="2024-05-29" version="1">MAAKMVITRLILCALSLSMIDFSMCYLLKSSVTCLDCPTGSDLAGIKILVKCNQVKNLAMATTNEHGVFETQLPSSNCQAKILGGPKQLYVPRNSMVTSIKMVQETGSYTTSQPLTFYTSCPLSQNNDGKCGAHNNVIGRNIGSSKTVDLPLPKEWGLAPSSYYVPFVPIIGIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C0M0</accession>
    <name>A0AAD5C0M0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">chitinase</fullName>
            <ecNumber evidence="4">3.2.1.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">M8C21_012715</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="19">AA19_3_7</strain>
            <tissue evidence="19">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Defense against chitin-containing fungal pathogens.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1-&gt;4)-beta-linkages in chitin and chitodextrins.</text>
            <dbReference type="EC" id="3.2.1.14"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyl hydrolase 19 family. Chitinase class I subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.14" evidence="4"/>
    <dbReference type="EMBL" id="JAMZMK010010072">
        <property type="protein sequence ID" value="KAI7733171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C0M0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008843">
        <property type="term" value="F:endochitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016998">
        <property type="term" value="P:cell wall macromolecule catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00325">
        <property type="entry name" value="chitinase_GH19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00035">
        <property type="entry name" value="ChtBD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.10:FF:000003">
        <property type="entry name" value="Class IV chitinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.20.10:FF:000001">
        <property type="entry name" value="Endochitinase (Chitinase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.530.10:FF:000052">
        <property type="entry name" value="Endochitinase PR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.530.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.20.10">
        <property type="entry name" value="Endochitinase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.10">
        <property type="entry name" value="Endochitinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001002">
        <property type="entry name" value="Chitin-bd_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018371">
        <property type="entry name" value="Chitin-binding_1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036861">
        <property type="entry name" value="Endochitinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016283">
        <property type="entry name" value="Glyco_hydro_19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000726">
        <property type="entry name" value="Glyco_hydro_19_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22595">
        <property type="entry name" value="CHITINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22595:SF193">
        <property type="entry name" value="ENDOCHITINASE EP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00187">
        <property type="entry name" value="Chitin_bind_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00182">
        <property type="entry name" value="Glyco_hydro_19"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001060">
        <property type="entry name" value="Endochitinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00270">
        <property type="entry name" value="ChtBD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="Lysozyme-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57016">
        <property type="entry name" value="Plant lectins/antimicrobial peptides"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00026">
        <property type="entry name" value="CHIT_BIND_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50941">
        <property type="entry name" value="CHIT_BIND_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00773">
        <property type="entry name" value="CHITINASE_19_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00774">
        <property type="entry name" value="CHITINASE_19_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="9" id="KW-0146">Chitin degradation</keyword>
    <keyword evidence="5 16" id="KW-0147">Chitin-binding</keyword>
    <keyword evidence="10 15 16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0611">Plant defense</keyword>
    <keyword evidence="13" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 17" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042105136" description="chitinase" evidence="17">
        <location>
            <begin position="23"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin-binding type-1" evidence="18">
        <location>
            <begin position="22"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="14">
        <location>
            <position position="128"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15 16">
        <location>
            <begin position="27"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15 16">
        <location>
            <begin position="32"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="84"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="146"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="233"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR001060-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR001060-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="KAI7733171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="265" mass="28653" checksum="1FF34F4F7D3DE6F8" modified="2024-05-29" version="1">MKIYEILLIAGVLLAGILNSAAQNCGCAPNLCCSRHGYCGTGVEYCGTGCQQGPCTTSTNNVDVASIVTDAFFNGIVNQAPGGCVGRGFYTRAAFLEALRSYGQFGRTGSADDSKREIAAFFAHATHETGQFCHIEEVDGRNNNYCDTGFPQYPCNPSKRYYGRGPLQLTWNYNYAQAGSANGFNGLNNPEIVATDRVISFRTALWYWMNSVRPVIGQGFGATIRAINGRLECDGANPATVSSRVRYYTQYCNQLGVAPGNNLQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C4S4</accession>
    <name>A0AAD5C4S4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_024546</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009738">
        <property type="protein sequence ID" value="KAI7734393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C4S4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7734393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="190" mass="21402" checksum="2846AF0CC48FC7A8" modified="2024-05-29" version="1">MAISQLNFHHQTLNNNPIHRFTTTFSHHAILRVHSFGLSSLTQHLVPEDKTRATKYNSSPNINKWVNFLPGRSWWDLMEEDEADQVKADNPVTVWYVLQRIWGLVADDKLVIYIGLCSLTFAAVSEISMPNLLAASVFAAQKGESLVLYRNVKILMFLCFVSGICSGLRSGCFGIANMVLVSESSWKLEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C551</accession>
    <name>A0AAD5C551_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Actin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">M8C21_028356</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">AA19_3_7</strain>
            <tissue evidence="6">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009527">
        <property type="protein sequence ID" value="KAI7735225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C551"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000148">
        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00190">
        <property type="entry name" value="ACTIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="KAI7735225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="76" mass="9010" checksum="EBD59828AA48DE05" modified="2024-05-29" version="1">MVGMGQKDAYISDETQPKRDIITVKYPIEHETIDNWDNLESMWRHLFYNELCLEPEKHQELLAKAPLNPKANRENI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C5Z9</accession>
    <name>A0AAD5C5Z9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_015399</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009439">
        <property type="protein sequence ID" value="KAI7735742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C5Z9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009725">
        <property type="term" value="P:response to hormone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009744">
        <property type="term" value="P:response to sucrose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004254">
        <property type="entry name" value="AdipoR/HlyIII-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20855">
        <property type="entry name" value="ADIPOR/PROGESTIN RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20855:SF121">
        <property type="entry name" value="ADIPOR_HEMOLYSIN-III-RELATED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03006">
        <property type="entry name" value="HlyIII"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="86"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="171"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="198"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="251"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="290"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="288"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="292"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR604254-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7735742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="351" mass="40980" checksum="5E8C36AAE19DD39A" modified="2024-05-29" version="1">MTSPISYHNIHVRVMNRKTQTPKMDETEYLNLNNKKMNNKYPLLRYEQLPDYMKDNEYILNYYRADWSLKHALISLFLCHNETLNVWTHLIGFVAFLVLTIANLTNLYEVADFLQVSKWVFSSDHSLNDTRQMISSYIDIPPPHIDVRRWPFLCHLFSCHSHHLNCRLSQLDYTGIAVMIITSFFPPIYYIFQCNPVWQYVYLGIITVLGIFVIMVLLSPTRMRAMGLFGVIPAIHGTIVNWNVPQRNTTLGFESAMGFSYLVGAMFYVSRVPEKWMPGLFDLVGHSHQIFHVFVVMGALAHYVSVLILYEYRRRIGYSHIDYLNIWTNHTIAMVTYKATTTLITRATIVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C6N7</accession>
    <name>A0AAD5C6N7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_020766</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009598">
        <property type="protein sequence ID" value="KAI7734976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C6N7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34190">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34190:SF4">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="26"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="94"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="33"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7734976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="139" mass="15699" checksum="3A6B0F85F2CD6910" modified="2024-05-29" version="1">MVVTTVEEPMLSRLDRLDKIMRQLEEIKGGSKTPKSSCASTPSTCTVTSDGQVSSVDFSPRSLEKHCRPIDDVIAETEMKGTLIERLVDVEDRVAKLCMQLQLEDEEKKRVEEEKKTVMKNKKKKGLKQLVKSCVTRSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C7Z0</accession>
    <name>A0AAD5C7Z0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_020603</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009252">
        <property type="protein sequence ID" value="KAI7736529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C7Z0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016279">
        <property type="term" value="F:protein-lysine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd10527">
        <property type="entry name" value="SET_LSMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1420.10">
        <property type="entry name" value="Rubisco LSMT, substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1410.10">
        <property type="entry name" value="set domain protein methyltransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036464">
        <property type="entry name" value="Rubisco_LSMT_subst-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050600">
        <property type="entry name" value="SETD3_SETD6_MTase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271:SF55">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271">
        <property type="entry name" value="UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="475"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="475"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="485"/>
            <end position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7736529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="534" mass="60204" checksum="D9009E8D87B2F94F" modified="2024-05-29" version="1">MGDTQESKLQLLLQWLKLNGVELRGCHIKYNDSNKGFAVYSSDAATDGVLLVVPLTLAITPMRVLQDPILGPVCSALFEEGEVDDRFLILLFLTFEFIRKNSSWKPYLDMLPTTFGNPLWFSDDEMSELRGTTLFKATELQKKSLQSLYDDKVKELVKKLLVLDGDLERANSIFWTRALSIPLPRSFVFPQIQEEEHNPATNPESANGDDVKKLEGDTSTIQEETVWVEGLVPGIDFCNHDLKAAATWEVDGTGSATGVPLSMYLLAVEEARLQIGEEISISYGDKGNEELLYLYGFVTDNNPNDYIMVHYPAEAIKDAPFSQTKLQLLEAQKAEMRCLLSKKLLDNGFFSNEKETRDKAKETEVVNYSWSGQRKTPSYLNKLVFPEEFLTCLRTIAMTEDEIFKVSSLLEELVDSEGERQPSDIEVRAAVWEACGDAGALQLLVDLLNTRMMDLEEGSGTEDFDTELLEKVRGYKNITPEDGKSNGTNEASKNQEMSRNKYASIVYRRGQKQLTRQFLKEAEHVLQLALSESN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C8Z6</accession>
    <name>A0AAD5C8Z6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">DUF21 domain-containing protein At3g13070, chloroplastic</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_007386</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009026">
        <property type="protein sequence ID" value="KAI7737432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C8Z6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04590">
        <property type="entry name" value="CBS_pair_CorC_HlyC_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1280.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002550">
        <property type="entry name" value="CNNM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044751">
        <property type="entry name" value="Ion_transp-like_CBS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005170">
        <property type="entry name" value="Transptr-assoc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22777">
        <property type="entry name" value="HEMOLYSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22777:SF17">
        <property type="entry name" value="UPF0053 PROTEIN SLL0260"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01595">
        <property type="entry name" value="CNNM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03471">
        <property type="entry name" value="CorC_HlyC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01091">
        <property type="entry name" value="CorC_HlyC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51846">
        <property type="entry name" value="CNNM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0129">CBS domain</keyword>
    <keyword evidence="6 8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 8 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="105"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="164"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="214"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="239"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="CNNM transmembrane" evidence="12">
        <location>
            <begin position="155"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="11">
        <location>
            <begin position="387"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="588"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="610"/>
            <end position="619"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7737432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="619" mass="68750" checksum="FF3935BE1BF0EA2C" modified="2024-05-29" version="1">MDVVTIEPSIFNNNNNNNNISFFKTIPQPSFFKSSTRRKTPIILHCAPNATQFNHKIILTNLHDSRFSTLVQKDRGLRSVLVKSNNLETPLNLGSNFLEILVKKGLILAAVCCGVFVLGCRGALAAEGVLNGGNIVGLEQIKLSMMNCMPKVLMVLKVLKEQGLILAALFGLSAFFSMAETSITTLWPWKVRELAEKESENGVFKMLRNDVTRFLTTILIGTTVVNIGATALVTEAATAIFGEAGVSAATGVMTVAVLLLTEITPKSIAVHNATEVARVVVRPIAWLSLVLYPVGRVVTYLSMGMLKLLGLKGRSEPYVTEDELKLMLRGAELSGAIEEEEQDMIENVLEIKDTYVREVMTPLVDVVASDSSATLIDFHTLWVTHQYSRVPVFEQRVDNIVGIAYAMDLLDYVQKVHMAVVLNEYGGTVGIVTLEDVVEEIVGEIFDENDSKEEIDKKTGYIVMRAEGIYDVDANTSIDQLSEDLNIKMPEGHQYETVSGFVCEAFGYIPKTGERIKVVLEKEEVDDEYIEDDGDRQHQKEKEKNQVFQIEILAGNARKVGAVRFERVTSEEEMDAKKVVIPVITKRKWGEDDDGGDDERNIIDEEDDVIPQHEIKEGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5C9E1</accession>
    <name>A0AAD5C9E1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">phosphoenolpyruvate carboxykinase (ATP)</fullName>
            <ecNumber evidence="4">4.1.1.49</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_011854</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>oxaloacetate + ATP = phosphoenolpyruvate + ADP + CO2</text>
            <dbReference type="Rhea" id="RHEA:18617"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.1.1.49"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the phosphoenolpyruvate carboxykinase (ATP) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.49" evidence="4"/>
    <dbReference type="EMBL" id="JAMZMK010009272">
        <property type="protein sequence ID" value="KAI7736459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5C9E1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004612">
        <property type="term" value="F:phosphoenolpyruvate carboxykinase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00484">
        <property type="entry name" value="PEPCK_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.8.10:FF:000001">
        <property type="entry name" value="Phosphoenolpyruvate carboxykinase (ATP)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.449.10:FF:000009">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.228.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.449.10">
        <property type="entry name" value="Phosphoenolpyruvate Carboxykinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.8.10">
        <property type="entry name" value="Phosphoenolpyruvate Carboxykinase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00453">
        <property type="entry name" value="PEPCK_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001272">
        <property type="entry name" value="PEP_carboxykinase_ATP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013035">
        <property type="entry name" value="PEP_carboxykinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008210">
        <property type="entry name" value="PEP_carboxykinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015994">
        <property type="entry name" value="PEPCK_ATP_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00224">
        <property type="entry name" value="pckA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006820">
        <property type="entry name" value="PRK09344.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006821">
        <property type="entry name" value="PRK09344.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30031:SF0">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE CARBOXYKINASE (ATP)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30031">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE CARBOXYKINASE ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01293">
        <property type="entry name" value="PEPCK_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68923">
        <property type="entry name" value="PEP carboxykinase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53795">
        <property type="entry name" value="PEP carboxykinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00532">
        <property type="entry name" value="PEPCK_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="5" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="10" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="34"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7736459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="665" mass="73255" checksum="AD2210701F890204" modified="2024-05-29" version="1">MASTGNGNVSGVTPKASGVTAGRNGLPKISTHKKQNEICHDDSSAPVKAQTIDELHSLQRKKSAPTTPMDGVQGAFANFTEEERHRQQLQSISASLASLTRETGPSLVKGDPARGPVTPRVQHAPDHYVAPTFSASDSSLKSTHVLYNLSPAELYEQAIKYEQGSFITSTGALATLSGAKTGRAPKDKRVVRDDVTEDELWWGKGSPNIEMDEHTFLVNRERAVDYLNSLEKVFVNDQFLNWDPENRIKVRIVSARAYHSLFMHNMCIRPTPEELESFGTPDFTIYNAGKFPCNRYTHYMTSSTSIDLNLGRREMVILGTQYAGEMKKGLFGVMHYLMPKRQILSLHSGCNMGKDGDVALFFGLSGTGKTTLSTDHNRYLIGDDEHCWSDKGVSNIEGGCYAKCIDLSREKEPDIWNAIKFGTVLENVVFDEHTREVDYLDKSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLSLAQTMYHFISGYTALVAGTEEGVKEPRATFSACFGAAFIMLHPTKYAAMLATKMEKHGATGWLVNTGWSGGSYGSGSRMKLAYTRKIIDAIHSGKLLSANYKKTEVFGLEIPTEIEGVPSEILDPVNTWSDKKAYNDTLLKLGGLFKNNFEVFVNHKIGKDDKLTEEILAAGPNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CA79</accession>
    <name>A0AAD5CA79_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_032882</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008850">
        <property type="protein sequence ID" value="KAI7738213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CA79"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055801">
        <property type="entry name" value="DUF7377"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF84">
        <property type="entry name" value="PROTEIN KINASE LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24093">
        <property type="entry name" value="DUF7377"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="339"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="32"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7738213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="595" mass="65798" checksum="FD48DF2209BBA572" modified="2024-05-29" version="1">MAAALECWSSRTSTDEDTVEQNLMRTTHRSESCSSSTLLETSSSSSSPTATVSVKDTSLMQKKLQKLTRNVSEAIASLKNSLNLDPARDSNPTRIDACRKNVWGSVVRNLTQLYPGSQLPEKLVSSIRKHFDSLPLSYSQAGFEMKDVFLHVRLIEQASGDDDPAFMIQEVSEDQVQGSVYKLTFACNSSLSWPVMSGSLDSASITCKKIQLFEKKGFTLGVVLLLVQPGQEKMFKSRVETALKTALKKPTKTNSMKLAFGLCGCQEEGTKGKQFGEIEDDCNDQSYRNEVEGLGSTCSKIKLQLPLSSSSIVISVDEWQTVQSGADEIKKWLLNPDSLEFVDQIGFGTFKGTYKGKKVGIEKLKGCEKGNSYEFEIRRDLLELMTCGHKNILQFYGVCIDDVHGLCVVNKLMEGGSVHDRIMVKNKKIQMKEVIRISADVAEGIKFMNDHGVAYRDLNTHRILLDKNGNACLGDMGIVSACKNAGEAMEYETDGYRWLAPEIIAGDPETVTETWMSNVFSFGMVVWEMMTGEAAYAALSPVQATVGIAACGLRPDIPKDCPQHLRSLLNKCWHNTPSKRPTFSEILSLLTRPNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CAH4</accession>
    <name>A0AAD5CAH4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_008441</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008822">
        <property type="protein sequence ID" value="KAI7738378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CAH4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11216:SF161">
        <property type="entry name" value="CALCIUM-BINDING EF HAND FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11216">
        <property type="entry name" value="EH DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="176"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="395"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="455"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="184"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="257"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="274"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="285"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7738378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="491" mass="54794" checksum="62F5BF6C8AEC4EE4" modified="2024-05-29" version="1">MQEIILYKSRCDSRLNEITERVSADRREVESLSKNYEDKYKQAGDVASKLTIEEATFRDIQEKKMELYRAIVKLDQDGKPDDIQARVDRIRADLEDQIKALNECSKMYGLRGKPTLLVELPFGWQVGIQEGAADWDENWDKFEDEGFTYVKELTLDVQNIIAPPKQKTLPVHNQSAFDDERPATSSSPSNSTNKSEKPPAANESVEPHDDSSVKTPPNSMKSTERPSKILENGSPGAIKKQSESVFSAEKTSDEAVNATSDNHYNSHSSWDFTATKHQDMDQESSHGNALFDSSDSWGLNPIRTNKNTITFDSVPSTPAYSYAGSPTGNNLFHNQGPFASSFTDSVPSTPAYSNAQRPFVSVFADSVPSTPMYDGPADHPFNNLSRFDSFTSNTPRDSFSRFDSFRSTAQDSESESNEGSFPSKNLARFDSMHSTAESDFGHSLFQPLESFSRFDSTRNTNDSSDSSHRYASFDDADPFGSHGPFKTSFDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CAP4</accession>
    <name>A0AAD5CAP4_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_033602</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PhzF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008854">
        <property type="protein sequence ID" value="KAI7738202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CAP4"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.310.10">
        <property type="entry name" value="Diaminopimelate Epimerase, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003719">
        <property type="entry name" value="Phenazine_PhzF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13774">
        <property type="entry name" value="PHENAZINE BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13774:SF17">
        <property type="entry name" value="PHENAZINE BIOSYNTHESIS-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02567">
        <property type="entry name" value="PhzC-PhzF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016184">
        <property type="entry name" value="PhzC_PhzF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54506">
        <property type="entry name" value="Diaminopimelate epimerase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7738202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="239" mass="26056" checksum="7C4B5E2EA2F51465" modified="2024-05-29" version="1" fragment="single">PVLGHGSENPRFHLRWFAPVAEVELCGHATLAASHFLYQSGLVNSNMIEFSTLSGILTAKRIPANKINGSSNGESEDSFYIELDFPVVPVSDYNDLEVSAISEILNGVSIVDVKKTTLDDIYVELSSAKEVAEFKPQIHKIKAAPGRGIVITALAPNGSGFDFYSRFFCPKYGIDEDPVCGSAHCALAAYWHEKLGKCDFVAYQASPRSGVLHVHLDKENQRVLLRGKATTVMEGSLLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CC39</accession>
    <name>A0AAD5CC39_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Zinc finger A20 and AN1 domain-containing stress-associated protein 8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_001599</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in environmental stress response.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008748">
        <property type="protein sequence ID" value="KAI7738704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CC39"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1110.10:FF:000001">
        <property type="entry name" value="Zinc finger AN1-type containing 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1110.10">
        <property type="entry name" value="AN1-like Zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035896">
        <property type="entry name" value="AN1-like_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050652">
        <property type="entry name" value="AN1_A20_ZnFinger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002653">
        <property type="entry name" value="Znf_A20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000058">
        <property type="entry name" value="Znf_AN1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10634">
        <property type="entry name" value="AN1-TYPE ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10634:SF104">
        <property type="entry name" value="ZINC FINGER A20 AND AN1 DOMAIN-CONTAINING STRESS-ASSOCIATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01754">
        <property type="entry name" value="zf-A20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01428">
        <property type="entry name" value="zf-AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00259">
        <property type="entry name" value="ZnF_A20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00154">
        <property type="entry name" value="ZnF_AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118310">
        <property type="entry name" value="AN1-like Zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51036">
        <property type="entry name" value="ZF_A20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51039">
        <property type="entry name" value="ZF_AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="A20-type" evidence="6">
        <location>
            <begin position="12"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="domain" description="AN1-type" evidence="7">
        <location>
            <begin position="104"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7738704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="169" mass="18223" checksum="2B72C1068176978D" modified="2024-05-29" version="1">MEKNETGCQTAPEAPTLCINNCGFFGTPSTMNMCSKCHKDMILQQEQVKLAASSSFNTILNGNNITSQSSIALSVSPVPEVALLAQTSSIPQAQAESGDKPNPGQGPSRCTTCRKRVGLTSFICRCGNVFCSVHRYSDKHDCQFDYRTTAKDAIAKANPVVKAEKLDKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CDP8</accession>
    <name>A0AAD5CDP8_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_028029</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008533">
        <property type="protein sequence ID" value="KAI7740011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CDP8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7740011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="92" mass="10295" checksum="5D2BDF87CBC58D19" modified="2024-05-29" version="1" fragment="single">MAENDDDDVELKIDPPVSDGNGGALFLRNNTVPSGIRMFVVKQNRFTIFFIRIPWKDQARTLAILVCGLTESKLESGMKREHVTSVSDRKAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CDQ5</accession>
    <name>A0AAD5CDQ5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">alcohol dehydrogenase</fullName>
            <ecNumber evidence="4">1.1.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">M8C21_018276</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="16">AA19_3_7</strain>
            <tissue evidence="16">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a primary alcohol + NAD(+) = an aldehyde + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10736"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15734"/>
            <dbReference type="ChEBI" id="CHEBI:17478"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a secondary alcohol + NAD(+) = a ketone + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10740"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17087"/>
            <dbReference type="ChEBI" id="CHEBI:35681"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the zinc-containing alcohol dehydrogenase family. Class-IV subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.1" evidence="4"/>
    <dbReference type="EMBL" id="JAMZMK010008504">
        <property type="protein sequence ID" value="KAI7740148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CDQ5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004022">
        <property type="term" value="F:alcohol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051903">
        <property type="term" value="F:S-(hydroxymethyl)glutathione dehydrogenase [NAD(P)+] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046294">
        <property type="term" value="P:formaldehyde catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08301">
        <property type="entry name" value="alcohol_DH_plants"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.180.10:FF:000067">
        <property type="entry name" value="alcohol dehydrogenase 1-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:001292">
        <property type="entry name" value="Alcohol dehydrogenase class-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000003">
        <property type="entry name" value="S-(hydroxymethyl)glutathione dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43880">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43880:SF40">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0520">NAD</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 13" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alcohol dehydrogenase-like N-terminal" evidence="15">
        <location>
            <begin position="35"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="Alcohol dehydrogenase-like C-terminal" evidence="14">
        <location>
            <begin position="206"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="Alcohol dehydrogenase-like C-terminal" evidence="14">
        <location>
            <begin position="406"/>
            <end position="532"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00060764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF08240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="KAI7740148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="575" mass="62842" checksum="234DD4DE97E5B554" modified="2024-05-29" version="1">MSTTTGHVIRCKAAVAWEAGKPLVIEEVEVAPPQKHEVRIKILFTSLCHTDVYFWEAKGQNPVFPRILGHEAGGVVESVGEGVTDLQPGDHVLPVFTGECKECAHCKSEESNMCDLLRINTDRGVMLHDQKSRFSINGKPIFHFVGTSTFSEYTVVHVGCLAKINPDAPLDKVCVLSCGISTGLGATLNVAKPKKGSSVAVFGLGAVGLAAAEGARIAGASRIIGVDLNANRFELAKKFGVTEFVNPKDYKKPVQEVIAEMTNGGVDRSVECTGHIDAMISAFECVHDGWGVAVLVGVPHKDAVFKTSPMNLLNERTLKGTFFGNYKPRSDIPSVVEKYMNKRSNTSMSTTTSQVIRCKAAVAWEAGKPLVIEEVEVAPPQKHEVRIKILFTSLCHTDVYFWEAKAAEGARIAGASRIIGVDLNANRFELAKKFGVTEFVNPKDYKKPVQEVIAEMTNGGVDRSVECTGHIDAMISAFECVHDGWGVAVLVGVPHKDAMFKTSPMNLLNERTLKGTFFGNYKPRSDIPSVVEKYMNKELELEKFITHEVPFSEINKAFDLMLKGEGLRCIIRMDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CDQ6</accession>
    <name>A0AAD5CDQ6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Sugar phosphate transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_014563</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008605">
        <property type="protein sequence ID" value="KAI7739559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CDQ6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004853">
        <property type="entry name" value="Sugar_P_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050186">
        <property type="entry name" value="TPT_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11132">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03151">
        <property type="entry name" value="TPT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="93"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="124"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="156"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="195"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="220"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="260"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="300"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="Sugar phosphate transporter" evidence="6">
        <location>
            <begin position="93"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="Sugar phosphate transporter" evidence="6">
        <location>
            <begin position="259"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7739559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="333" mass="36508" checksum="EED8F763C4D14843" modified="2024-05-29" version="1">MESCALRLSSSILNPKPSNIRHRIIPSSSPSTINYKTTHSDLSLTSYNSIFNNSFSLNFQKRIHYDFAVASAAASASEPSSQSTNPAEFGPTLQLAAMFGIWYLLNIYFNIFNKQVLKVFPYPTTLTTFQFGCGTLMVLIMWTLKLHPRPKFHKSQIVPVLVLGVTHTMGNLLTNVSLGKVSVSFTHTIKALEPFFTVLFSVLLLSETPTIWVISSLLPIVSGVALASFTEASFNWIGFASAMASNVTNQSRFKLTPEHLQLAISYMILAMVSPVTHAVGNCVKRVVVIVSSVIFFQTPVSPINSLGTGLALAGVFLYSRVKRIKPLLKPKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CE64</accession>
    <name>A0AAD5CE64_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_021680</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008481">
        <property type="protein sequence ID" value="KAI7740251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CE64"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="11"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="180"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="214"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="274"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="284"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="381"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="474"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="502"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="526"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="549"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="591"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7740251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="591" mass="66233" checksum="B0F0BE5108B05463" modified="2024-05-29" version="1" fragment="single">SSDSESESDSDSSSSSSSSSSSSDLSSSSDDRRKRKKRPSKRDRHKRGKRRDKRHAKKRRKRDKKSKRKLKRGSDSSSKSASENEHGSEDNGDARKPNQKHNNSAKITDDNQSPSAEVEDGSGRHKTKDAFEREEVEFPKENGNRPINGTDVVMVGRPDVANDHPCNSRSISKSPRRALSKSMSISPRRSPSMSPRRNLSRSPSVGGSHGRATSLKNWSPVRSVSKNKSPVRRISRSPIREKNVRSISPSPVRSQIRKPVGRNSTPSPSPSPPRSRKSSRKSVSRSPLRSSRVSYSRSPVRSSKQSLSRSPVRSSKRSLSGSPVRPSKRSLSGSPVRSSKRSLSRSPVRSSKRSLSRSPVRSSKRSLSRSSGRPPLRRSLSRSPVRPPARSSRRSPSRSPVGGHRGRSPSRSVSPDGSPKRIRRGRGFSNRYSYARRYHSPDRSPVRPYRYGRSDHDRYSGYRRRSPVRDRSPLRYRGRRSRTRSPSVSRSPVRYRRRYSRSRSPVGTSRYRPSPPVERRRRAPRSRTPPSRSRSPYEPRSSPSPSPRRTSRSKSKSMSRSRSRSRSSSGAPPAKEGLVSYGDGSPDSGQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CG37</accession>
    <name>A0AAD5CG37_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_016601</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008227">
        <property type="protein sequence ID" value="KAI7741272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CG37"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7741272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="66" mass="7312" checksum="CD1C506135773E8A" modified="2024-05-29" version="1">MQGFCLSLPRILRRTFVACNDVGLSLKTLLPVVAASACAAAIAFRAFRCRIPHALHSDYKEKIMTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CH45</accession>
    <name>A0AAD5CH45_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Rad21/Rec8-like protein C-terminal eukaryotic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_030198</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="6">Component of the cohesin complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the rad21 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008348">
        <property type="protein sequence ID" value="KAI7740780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CH45"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008278">
        <property type="term" value="C:cohesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990414">
        <property type="term" value="P:replication-born double-strand break repair via sister chromatid exchange"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007062">
        <property type="term" value="P:sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.580:FF:000002">
        <property type="entry name" value="Sister chromatid cohesion 1 protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.580">
        <property type="entry name" value="Structural maintenance of chromosome 1. Chain E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039781">
        <property type="entry name" value="Rad21/Rec8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006909">
        <property type="entry name" value="Rad21/Rec8_C_eu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023093">
        <property type="entry name" value="ScpA-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12585">
        <property type="entry name" value="SCC1 / RAD21 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12585:SF55">
        <property type="entry name" value="SISTER CHROMATID COHESION 1 PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04824">
        <property type="entry name" value="Rad21_Rec8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="3" id="KW-0498">Mitosis</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rad21/Rec8-like protein C-terminal eukaryotic" evidence="7">
        <location>
            <begin position="37"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00064543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7740780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="94" mass="10295" checksum="8A2582A75FE69AD0" modified="2024-05-29" version="1">MRGTPEPGSLLPRTRGVAKLIKEKSSATPSTSDSLGSVSMNSILEGKTRKICSRMFFETLVLKSCDLIDVKQDEPYGDITLKVTPMLSKLDFSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CIH2</accession>
    <name>A0AAD5CIH2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">TIR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_018474</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008255">
        <property type="protein sequence ID" value="KAI7741156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CIH2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044974">
        <property type="entry name" value="Disease_R_plants"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11017:SF479">
        <property type="entry name" value="DISEASE RESISTANCE PROTEIN (TIR-NBS-LRR CLASS) FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11017">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01582">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TIR" evidence="1">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7741156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="301" mass="34718" checksum="563CB8D1AB77AB30" modified="2024-05-29" version="1">MECHNKMGQKVIPVFYHVDPSDIRGQKNQVAVFFQQHEERFRQELDKVKEWRDALTAAANLSGFHISETFKEGEYSYIRKIVEEISADIQPPSMETIKVSDEQYDKGFSANVFKHMKNLRLLDVRGKFTSSKPTFFHDNLRWLSWSRYPFSSLSMTHMNKLVRLEVVGGTIKQFWNGQKIMPSLKIVKLEELDYLTTFPDVSGAPNIEKLVVRNCKKLVKFHKSLGSHKRISELEVTGCNRLKGLPSRVEMESLTFLTVKECSSLERFPALSPCMEEMKVLGKSTLQFPMNIQKGMDTGKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CJK5</accession>
    <name>A0AAD5CJK5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Germin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_018596</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Secreted</location>
            <location evidence="1 12">Extracellular space</location>
            <location evidence="1 12">Apoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the germin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007993">
        <property type="protein sequence ID" value="KAI7742395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CJK5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048046">
        <property type="term" value="C:apoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02241">
        <property type="entry name" value="cupin_OxOx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000047">
        <property type="entry name" value="Auxin-binding protein ABP19a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006045">
        <property type="entry name" value="Cupin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001929">
        <property type="entry name" value="Germin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019780">
        <property type="entry name" value="Germin_Mn-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31238">
        <property type="entry name" value="GERMIN-LIKE PROTEIN SUBFAMILY 3 MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00190">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00325">
        <property type="entry name" value="GERMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00835">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00725">
        <property type="entry name" value="GERMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 12" id="KW-0052">Apoplast</keyword>
    <keyword evidence="7 11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8 9 12" id="KW-0464">Manganese</keyword>
    <keyword evidence="5 9 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041784213" description="Germin-like protein" evidence="12">
        <location>
            <begin position="24"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="Cupin type-1" evidence="13">
        <location>
            <begin position="58"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>oxalate</name>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="29"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR601929-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU366015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7742395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="215" mass="22176" checksum="AD5C48E8E3FC372F" modified="2024-05-29" version="1">MLHQTSVVVVVFCFLLVASSSYASVQDFCVADLSLPDTPSGFPCKDVKKVSVSDFVYSGLAAAGNTSNIIKAAVTPAFTAQFPGVNGLGISTARLDLAVGGVIPIHTHPGGSELLVVIQGTICAGFISSSANKVYFKTLNKGDVMVFPQGLLHFQINSGKAPALAIVSFSSPVPGLQITDFALFANDLPTEIVAATTFLDVAQIKKLKGVLGGSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CJW2</accession>
    <name>A0AAD5CJW2_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_008886</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010008107">
        <property type="protein sequence ID" value="KAI7741904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CJW2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46422">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE BSL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46422:SF4">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE BSL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7741904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="150" mass="15432" checksum="C26B543C348E92BB" modified="2024-05-29" version="1">MVRSPVKCQCSSSVSGMSENSGLLSQVACGFLVMQDHGLVWMIGSPAGVWCDTKSVVTSPRTARYSADAAGGDAAVELTRRCRHAAAVGDLIFIYGGLRGGVVLDDLLVVEDLAAAEMTSAASHAAAAAAAANAQVGRPPGWYGFVDDRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CKH6</accession>
    <name>A0AAD5CKH6_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_024872</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007766">
        <property type="protein sequence ID" value="KAI7743234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CKH6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051058">
        <property type="entry name" value="GDSL_Est/Lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45648">
        <property type="entry name" value="GDSL LIPASE/ACYLHYDROLASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_4G14700)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45648:SF13">
        <property type="entry name" value="OS02G0290900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7743234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="277" mass="31468" checksum="FF769FCB2C273D07" modified="2024-05-29" version="1">MMRGLNYASAGAGIIFSSGSELGQHISFSQQIQQVMDTFQQFILTMGEHAAADHISNSVFYISIGTNDFIHYYLPDVSGVQSKFFAWRFTQFLAQTMKQEIKNLYNANVRRVVVMGLAPIGCAPYYLWQYNSQNGECVKPINNVILEFNFAMRYAVDELNKELVDANIIFCDAYTGSMDILKNKEQYGFSYTDTACCGFGRYNGWLMCLSSGMACRNATNHLWWDQFHPTSAVNEILADNVWSGLHTDMCYPMNMQQMVAQRVTMSDDAINRYPNQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CMD9</accession>
    <name>A0AAD5CMD9_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-CH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_017205</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007495">
        <property type="protein sequence ID" value="KAI7744578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CMD9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16495">
        <property type="entry name" value="RING_CH-C4HC3_MARCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022143">
        <property type="entry name" value="DUF3675"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033275">
        <property type="entry name" value="MARCH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23012:SF176">
        <property type="entry name" value="OS01G0894600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23012">
        <property type="entry name" value="RING/FYVE/PHD ZINC FINGER DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12428">
        <property type="entry name" value="DUF3675"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12906">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51292">
        <property type="entry name" value="ZF_RING_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-CH-type" evidence="4">
        <location>
            <begin position="45"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7744578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="200" mass="22387" checksum="39F07C0E519E997D" modified="2024-05-29" version="1">MTASSCFHSTQQDRMISQVVQMLQPNSKINENKGDLLTNVAGEGCSKTVIRECRICQEEDDEHQLESPCACNGTLKFAHRKCIQKWCNKKGDITCEICNQVYSPDYALPPARTNSDVMTIDIRHAWGPQIDLRDAHFLAFASAENQFLEPDYDDYGVASNNSIAYLRFMALIVIDDNIADTPSFAGHERPGDAARIIKVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CNV3</accession>
    <name>A0AAD5CNV3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_029903</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
            <location evidence="8">Cytoskeleton</location>
            <location evidence="8">Phragmoplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. KIN-7 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007635">
        <property type="protein sequence ID" value="KAI7743901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CNV3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009524">
        <property type="term" value="C:phragmoplast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000919">
        <property type="term" value="P:cell plate assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01374">
        <property type="entry name" value="KISc_CENP_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000016">
        <property type="entry name" value="Kinesin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021881">
        <property type="entry name" value="NACK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968">
        <property type="entry name" value="CENTROMERE PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968:SF11">
        <property type="entry name" value="KINESIN MOTOR DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11995">
        <property type="entry name" value="DUF3490"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="2 10" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6 9 10" id="KW-0505">Motor protein</keyword>
    <keyword evidence="3 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="13">
        <location>
            <begin position="17"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="516"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="558"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="607"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="350"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="519"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="607"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="618"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="629"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="640"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="105"/>
            <end position="112"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00060413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7743901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="857" mass="96415" checksum="0AAFEC615C542DBF" modified="2024-05-29" version="1">MSISGDEMKPGDGQEEKIFVTVRVRPLNAKEIAKNDSSYWECVSNHTIIYKNVKALTERSMYPNAYTFDRVYGHDSTTREVYEDGVKRIALSVLNGFNSSVFAYGQTSSGKTYTMTGITQYAISDIFDHISKQDDREFMLKFSAIEIYNECVRDLLSADATPLRLLDDPEARTVVDKLTEINLEDWTHLMELLAVCEAQRKIGETSMNEMSSRSHQIIRLTIESSPSEFSGIDSASILAATVNFIDLAGSERASQSLSMGKRMKEGGHINRSLLTLGTVIRKLSKEPNGHIPYRDSKLTRILQNSLGGNAKTAIVCTLSPAHAHLEQSRNTLLFAVCAKEVRTSAQINVVMSEKALVKQLQQEMARLENELKNISGPTNDSEAIIKEKELQILKMEQEIKELTRERDLARSRLDEILRAAGVDQNSLPWTDSCSWDGGSSSGHLRDSSFDGHSTSDTSDVAVNVKVKVNAPLPPIALVNTYSFPKNHKLHNPNEDHYVFDNITPREFMNQYFGPDPTQEWDKTSQKARERTDSIDSCNEVEECIEKDEADDIVDDTHDENQIEGDFGNSHADQSTRSAHEDVLETKAPSLTRSTSCSAVIEGDVLSSPVSEKSVVAESKSESDIKSLCRQDSQGSTTSVNEKEMQHTDGDIFISSPSSNTQEKVMVKATPETIESGDEMEGYDSEKDWCLLFEEQRKKITKLWNECNIPLLHRTYFFLLIKGDPSDSVYIEVELRRLSFLKKTLDQVASARALNLERAMLSRKLLKKFTSTERQAMFAKWGIDLESRNRRFQLSQLLWTKTDDIEHVRDSAEIVAKLVGLIEPDKTPKELFGLTVATKQESQSLLSAWKNNLSLLRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CQ71</accession>
    <name>A0AAD5CQ71_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_024658</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007046">
        <property type="protein sequence ID" value="KAI7746093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CQ71"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025886">
        <property type="entry name" value="PP2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32278">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32278:SF111">
        <property type="entry name" value="F-BOX PROTEIN PP2-B12-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14299">
        <property type="entry name" value="PP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7746093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="223" mass="25418" checksum="A63BA91DD609CEEF" modified="2024-05-29" version="1">MSCPNLLNTGNDDNVLIPLDLLSNIIFFGNNVLVTSIDEQLVSLSKVNEKKCHTLPAKMVLYESSNVKCFKWKSPVESSVRDASMGRGKVFHFEEVAEFISHQVFEIKCEIETQKLSPDTDYACHLIFKLSEKCHGLHCPVKVQDLIDRKNKDAKFLYFRSPRFVHLQGNGRVPKHREDGLMEVIVWEFNSGNDDHVPMSLKLRCYEGNMSGLIVHGVEIRPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CQ96</accession>
    <name>A0AAD5CQ96_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_026861</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010007065">
        <property type="protein sequence ID" value="KAI7745997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CQ96"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7745997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="72" mass="8058" checksum="30BD232BFBB82AB0" modified="2024-05-29" version="1" fragment="single">MFEKPTIEVMMILLITVVNIACSMLMHVTCSMLIQARSRWWGCCSTYFKGTLMKNESQDTVKTGLGCSSMVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="9" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CR16</accession>
    <name>A0AAD5CR16_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Hexosyltransferase</fullName>
            <ecNumber evidence="13">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_016245</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2 13">Glycan metabolism; pectin biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Golgi apparatus membrane</location>
            <topology evidence="1 13">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the glycosyltransferase 8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="13"/>
    <dbReference type="EMBL" id="JAMZMK010007018">
        <property type="protein sequence ID" value="KAI7746167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CR16"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047262">
        <property type="term" value="F:polygalacturonate 4-alpha-galacturonosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06429">
        <property type="entry name" value="GT8_like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000056">
        <property type="entry name" value="Hexosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029993">
        <property type="entry name" value="GAUT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002495">
        <property type="entry name" value="Glyco_trans_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32116">
        <property type="entry name" value="GALACTURONOSYLTRANSFERASE 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32116:SF20">
        <property type="entry name" value="HEXOSYLTRANSFERASE GAUT11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25557">
        <property type="entry name" value="GAUT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01501">
        <property type="entry name" value="Glyco_transf_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 13" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 13" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9 13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="16"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU362027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7746167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="532" mass="60963" checksum="D9B7350AA27646FE" modified="2024-05-29" version="1">MRRRAADYRRPARRRFPVWIWVVIGLLFCTAGFILLVLKQNQDHHPTPVRNGGTLQSVHNNLNLTKETTNSNSYARQLLDQMTLAKAYIVIAKEHNNHHLAWELASKIRSGQFLLSKAAMRDSPMTQNEAEPLIRSLSTLIYKAQDAHYDIATTIMTMKSHIQALEERANAATVQSTFFGQLAAESVPKNLHCVNVKLLATWLTTKKLHKVTNEIKNSPKLVDNNLYHFCIFSDNLLAVSAVVNSTVSNADHPKRLVFHIVTNEPNYGAMQAWFVSNDFKGSMIEVQKVEDFTWLNASYSPIVKKLTQTDFQEYYIEGSQDLNNEPKFENPKYVSLLNHLRFYIPEIYPHLEKVVFLDDDVIVQKDLTPLFSLDLHGNVNGAVETCLEAFHRFYKYFNFTNPVLSSKFDPQACGWAFGLNVFDLVAWRNANVTDVYHYWQEQNGDGSLWKLGALPAGLLAFYGRTEPLDRRWHLLGLGYDVNIDNRIIESAAVVHFNGNLKPWLKFGIGRYKPLWERYVNQTHPCLLDCVTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CS26</accession>
    <name>A0AAD5CS26_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">S-type anion channel SLAH1-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_007711</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SLAC1 S-type anion channel family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006894">
        <property type="protein sequence ID" value="KAI7746804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CS26"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008308">
        <property type="term" value="F:voltage-gated monoatomic anion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006873">
        <property type="term" value="P:intracellular monoatomic ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.150">
        <property type="entry name" value="Voltage-dependent anion channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030183">
        <property type="entry name" value="SLAC/SLAH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004695">
        <property type="entry name" value="SLAC1/Mae1/Ssu1/TehA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038665">
        <property type="entry name" value="Voltage-dep_anion_channel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31269">
        <property type="entry name" value="S-TYPE ANION CHANNEL SLAH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31269:SF55">
        <property type="entry name" value="VOLTAGE-DEPENDENT ANION CHANNEL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03595">
        <property type="entry name" value="SLAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="64"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="93"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="123"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="147"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7746804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="190" mass="21046" checksum="A9F6CEB99DF2B90E" modified="2024-05-29" version="1">MVANPTSQLSVIGNLVGAHAAIKMGWTESGICLFTLGMTHYLVVFITLYQRFSGNNHIPSRLRPVFFMFVATPSMAALAWKSINGNFDMLCKMLFFLSLFLFTSLASRPVLFKKSMKNFSVAWWAFSFPLSFLALASMAYAQQAKEATATCLALVLSGISVLVFVFLLVCSTLKIDSLLRKPILNFSNES</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CSA7</accession>
    <name>A0AAD5CSA7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HTH myb-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_012765</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006906">
        <property type="protein sequence ID" value="KAI7746722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CSA7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015495">
        <property type="entry name" value="Myb_TF_plants"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10641:SF1277">
        <property type="entry name" value="HOMEODOMAIN-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10641">
        <property type="entry name" value="MYB FAMILY TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13921">
        <property type="entry name" value="Myb_DNA-bind_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7746722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="66" mass="7711" checksum="F73777A91A5A234B" modified="2024-05-29" version="1" fragment="single">TKMEPGKAHGTKRKMRSYEPIFRGLSRSGNSCRLQWMNYVRPNMKHGNFTKEEDMVCDSFKVVGKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CV25</accession>
    <name>A0AAD5CV25_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_032177</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006881">
        <property type="protein sequence ID" value="KAI7746885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CV25"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7746885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="114" mass="12984" checksum="C510C3D57B691B07" modified="2024-05-29" version="1" fragment="single">MTLMYQVEYLHIKAWLTMQDIGMSSEALFDDKTQESSDVSVNEKSEVVTIDDSPRNRPKIDVPEEENRLLMEVKETSILSWLQNFENGVTLADILKHFTGSTEAAVVDVLNCME</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CWG2</accession>
    <name>A0AAD5CWG2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_030126</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006321">
        <property type="protein sequence ID" value="KAI7749613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CWG2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01959">
        <property type="entry name" value="nsLTP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.110.10">
        <property type="entry name" value="Plant lipid-transfer and hydrophobic proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036312">
        <property type="entry name" value="Bifun_inhib/LTP/seed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016140">
        <property type="entry name" value="Bifunc_inhib/LTP/seed_store"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033872">
        <property type="entry name" value="nsLTP2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33214">
        <property type="entry name" value="BIFUNCTIONAL INHIBITOR/LIPID-TRANSFER PROTEIN/SEED STORAGE 2S ALBUMIN SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33214:SF44">
        <property type="entry name" value="NON-SPECIFIC LIPID TRANSFER PROTEIN GPI-ANCHORED 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00234">
        <property type="entry name" value="Tryp_alpha_amyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00499">
        <property type="entry name" value="AAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47699">
        <property type="entry name" value="Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042158208" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain-containing protein" evidence="3">
        <location>
            <begin position="25"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="Bifunctional inhibitor/plant lipid transfer protein/seed storage helical" evidence="4">
        <location>
            <begin position="30"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7749613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="95" mass="9975" checksum="50140EAE4CB7D350" modified="2024-05-29" version="1">MRPFTYTSLCAAAILVMILAGSQAAMAVTCQVTQLVPCASAISSSSPPSSQCCTKLKEQRPCLCQYMKNPSLRGYVSSPNAQKVAKACGVPTPQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CWG8</accession>
    <name>A0AAD5CWG8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">M8C21_004273</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="14">AA19_3_7</strain>
            <tissue evidence="14">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006389">
        <property type="protein sequence ID" value="KAI7749282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CWG8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20653">
        <property type="entry name" value="CYP81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000023">
        <property type="entry name" value="Cytochrome P450 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050651">
        <property type="entry name" value="Plant_Cytochrome_P450_Monoox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47947">
        <property type="entry name" value="CYTOCHROME P450 82C3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47947:SF62">
        <property type="entry name" value="CYTOCHROME P450, FAMILY 81, SUBFAMILY D, POLYPEPTIDE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11 12" id="KW-0349">Heme</keyword>
    <keyword evidence="8 11 12" id="KW-0408">Iron</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 12" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="7 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="11">
        <location>
            <position position="439"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="KAI7749282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="503" mass="57206" checksum="E8AFA0E047F9D385" modified="2024-05-29" version="1">MKETHIFFISFLCLVPLIYFVKLLTHIRGKNLPPSPPSIPIIGHLHLINGHVHRVFQHLSNKYGPVMALRFGSRPVVVITSPSAVEECFTKNDIILANRPLLLSGKYLDYDHSTVGAAPYGQLWRDLRRIMTLELFSTARLKSYSSVREDEVRSLIKNLYLDCFEDFTKVEMRSRIQGLSFNIVMKMIADKRFFGTGIEDLAEASQFKDVIREVFEVSGASNPGDFIPFLRWMDFQGYEKRLLKLQKKCDRFSQNLIEEIRTKRSSERGNEKTFIDAMLSLQQSEPEYYTDDLIKGNILTLLQAGTDTSSVTIEWAMALLLNHPDVLNRARVELDNDIGQTRLVEETDLTNLPYIQYIVNETLRLFPAGPLLVPHEPSQDCTIGGFDVARGTIVLVNAWAIHRDPVVWDEPLSFKPERFEKLGNDGNRFIPFGMGRRQCPGSGLANRIMVLALASLIQCFEWERVGEELVSLSEGKGLTMPKDIPLEAMCRGRQGMSHLIKAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2024-11-27" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CXH4</accession>
    <name>A0AAD5CXH4_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Serine/threonine protein phosphatase 2A regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_030901</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the phosphatase 2A regulatory subunit.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006391">
        <property type="protein sequence ID" value="KAI7749257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000331">
        <property type="entry name" value="Phosphoprotein phosphatase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002554">
        <property type="entry name" value="PP2A_B56"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10257">
        <property type="entry name" value="SERINE/THREONINE PROTEIN PHOSPHATASE 2A PP2A REGULATORY SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10257:SF103">
        <property type="entry name" value="SERINE_THREONINE PROTEIN PHOSPHATASE 2A REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01603">
        <property type="entry name" value="B56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028043">
        <property type="entry name" value="PP2A_B56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR028043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7749257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="384" mass="45178" checksum="47EC577256BC4B03" modified="2024-05-29" version="1">MKRQTLLELADYLASSNVKFTESVIQEFVKMVSANLFRELSPQPXXXXXXXXXXXXXXXXXXXSWPHLQIVYELLLRFIASPEADAKIAKKFIDHYFVLKLLDLFDSEDPREREYLKTILHRVYGKFMVHRPFIRKSINNIFYRFVLETEKHNGIAELLEILGSIVNGFALPLKEEHKLFLSRALIPLHKPKCLAMYHQQLSYCITQFVEKDCKLADMVIRGLLKYWPVTNSSKELMFLGELEEVLETTQQPQFHRCMVPLFKQIARCLNSLHFQVAERALFFWNNDHIESLIRQNRKVILPIIFPALERNGRYHWNQAVHSLTLNVRKIFFDLDPELFNECLLNFQEDEAKEEKIQSRRESTWRHLEEIAADKASSNEAVLVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5CYU0</accession>
    <name>A0AAD5CYU0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_028950</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010006150">
        <property type="protein sequence ID" value="KAI7750479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5CYU0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040008">
        <property type="term" value="P:regulation of growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044194">
        <property type="entry name" value="BLISTER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47490">
        <property type="entry name" value="PROTEIN BLISTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47490:SF2">
        <property type="entry name" value="PROTEIN BLISTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="19"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="151"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="492"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="630"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="324"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="436"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="24"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="43"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="80"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="155"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="504"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7750479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="666" mass="73466" checksum="B4B79EA4949ACDE9" modified="2024-05-29" version="1" fragment="single">IFFLHLQLEEFRKKKAAAKKAAASNSVHSANGELQDTKPPVSDSVQVITDATSDSGSGFHVGKNEKKTTNVVEAEPDVNPISSFNKPSASPGPTYSTDGKLDHKAPYEVDTEKNKEFNHPEENIYSSYNHFRNIRQEDSFKNSRDERLNDFASATPVTTQVSVTKSSPERSNGSLFASNLSYSNHQPANGPTYRDPSSEARKSIQDMLESRYPPVSSVDDKSLFDYPAKATSSSLWSSESANVRSEAIPSYNQASMSPGLAGRRSRPSFLDLINVPKNAETDKDNPFSSKVHPVDAHVTSDSQNSIFRQGINDFDMEKKNNFDSTKQNEDFAALEQHIEDLTQEKFSLQRALEASRVLAESLATENSALTDSYNQQGGLVNQLKAEIESLQDEINAQLVELQAVRTEYGNAQLECSAADERAKLLASEVIGLEEKALRLRSNELKLERQLENLEAEMSSQKRKISSLEKDRQDLQSTIHALQEEKKLMQSKLWNSPAIGGNSVDTKKSPTARKEASTSTDDLDESEDAPTNLATQASLGDGDAFLPSLSRNLGHEVSSSSIPSDQMYMIQNINTLISELALEKQQLVQALSTESSLSSKLKDLNKELSHKLEVQTQRLELLTSQSMVSDNIPQRKPVPRNVVDNTPYADEGDEAHAGALTTALPQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D0M2</accession>
    <name>A0AAD5D0M2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Eukaryotic translation initiation factor 3 subunit B</fullName>
            <shortName evidence="6">eIF3b</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">eIF-3-eta</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">eIF3 p110</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_011254</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the eIF-3 subunit B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005800">
        <property type="protein sequence ID" value="KAI7751846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D0M2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005852">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12278">
        <property type="entry name" value="RRM_eIF3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000286">
        <property type="entry name" value="Eukaryotic translation initiation factor 3 subunit B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03001">
        <property type="entry name" value="eIF3b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011400">
        <property type="entry name" value="EIF3B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034363">
        <property type="entry name" value="eIF3B_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013979">
        <property type="entry name" value="TIF_beta_prop-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14068">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 EIF3 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14068:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08662">
        <property type="entry name" value="eIF2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036424">
        <property type="entry name" value="eIF3b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 6" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5 6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6 7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="55"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7751846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="634" mass="73087" checksum="8EFA4718507EA81D" modified="2024-05-29" version="1">MVDVMSMSFDDIITESSRLNIGSVILPPGEDFGIKSDDEDVYEDESLECDAGFRSVIVVDNLPVVPQKKFDRLEAVVRRRFSRFGVIKEKGVWMPVEEDTGKTRGYCFIEYNTPQENLQRWLVDEKGRDQFVIRSGSDTEFSTESFVQWSPLGTYLAMVHRQNAAVWGGAFGFNQLMRYVHPQVKLIDFSPGEKYLVTCSSHELSQPCDTHRVVLNIFDVRTGNVMKEFKESADEFAFGETGGFTGVSWPVFRWSGGNEDKYFARLGKNVISVYETKTFNLIDTKSIKVENVMDSCWSPTDSIIALFVPELGGGNQPARVSLFQIPSKVELRQKNLFSVNDCKMHWQSNGEYLVVKVDRYTKTKKNTYTGFELFRIKERDIPIETFELDNKNDKVISFAWEPKGHRFAVIHGDNPRPDVSFYSVKGGKVSKLTTLKQKQANALFWSPGGCFIILAGLKGFNGQLEFYNVDELVTIATAEHFMATNVEWDPTGRYVATSVTSVHEMENGFNIWTLNGKLLYHIPKEHFFQFAWRPRPASLLSPEKEEEILKNLKKYSKKYEREDQDISVLLSEQEREKRKRLKEEWEMWVNEWKSYHEEQMLRDVEASDVEDDKQVEVEETLDVTEEILPDVSVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D1I5</accession>
    <name>A0AAD5D1I5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Nuclear transcription factor Y subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">M8C21_010554</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">AA19_3_7</strain>
            <tissue evidence="10">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Heterotrimeric transcription factor composed of three components, NF-YA, NF-YB and NF-YC. NF-YB and NF-YC must interact and dimerize for NF-YA association and DNA binding.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the NFYA/HAP2 subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005857">
        <property type="protein sequence ID" value="KAI7751667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D1I5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016602">
        <property type="term" value="C:CCAAT-binding factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018362">
        <property type="entry name" value="CCAAT-binding_factor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001289">
        <property type="entry name" value="NFYA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12632">
        <property type="entry name" value="TRANSCRIPTION FACTOR NF-Y ALPHA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02045">
        <property type="entry name" value="CBFB_NFYA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00616">
        <property type="entry name" value="CCAATSUBUNTB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00521">
        <property type="entry name" value="CBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00686">
        <property type="entry name" value="NFYA_HAP2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51152">
        <property type="entry name" value="NFYA_HAP2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="3 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 8" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="237"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="287"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="255"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU367155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="KAI7751667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="307" mass="33966" checksum="B13DD515C95CF905" modified="2024-05-29" version="1">MQELLTNNCDPCGVGFPSQWTGGGESFADQSTLSDNLSLKMGSSTPHQQNTKQVGFQFQFQDQDSSSTQSTSQSYPEVASAGDSCKYWEKISVQSAHGTHEDGSVGSALPIGTQDFAFASQPDHKQPYACIPLPYYHGMFAPYGYGSQTMLPHFQGMNITSTRIPLPPDFTQGEAIYVNAKQYSAILRRRQQRAKLEAQNKLLKPRKPYLHESRHVHALKRARGPGGRFLNLKKIQETQPDGSSNEQEDMDPASLETNLHGGSSDSTTASNIDNYFHQHELKFSIYNSHSDGPTQADGFGGTHHMFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D2V9</accession>
    <name>A0AAD5D2V9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_020278</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005523">
        <property type="protein sequence ID" value="KAI7753173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D2V9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7753173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="95" mass="10248" checksum="948344297C535453" modified="2024-05-29" version="1">MSGNDGNGGDDRERLPAASMSVTSVLYMKDGVSIVTAGEVDSRFPVRMDVATCSSSNYGQNDTNIKKTNSRLPFLNLNLNMLPDCRPAAKRGVSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D3M0</accession>
    <name>A0AAD5D3M0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">M8C21_010149</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">AA19_3_7</strain>
            <tissue evidence="7">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005361">
        <property type="protein sequence ID" value="KAI7753631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D3M0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.80">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003441">
        <property type="entry name" value="NAC-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036093">
        <property type="entry name" value="NAC_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31744:SF220">
        <property type="entry name" value="LOW QUALITY PROTEIN: NAC DOMAIN-CONTAINING PROTEIN 90-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31744">
        <property type="entry name" value="PROTEIN CUP-SHAPED COTYLEDON 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02365">
        <property type="entry name" value="NAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101941">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51005">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NAC" evidence="6">
        <location>
            <begin position="8"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="176"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="195"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="KAI7753631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="207" mass="24187" checksum="983D9D735B22CB97" modified="2024-05-29" version="1" fragment="single">AEQDLHLNSVIYRFYPTEEELISFYLLNKLQNRRTHELHRVIPAVNVYEHNPSHLPRLAGELCQRDTEQWFFFVPRQEREEQGRRPSRTTASGFWKATGSPTYVYSSENKVIGVKKTMVFYEGKSSKVKKTEWKMNEYRAIEEEVDVTNNLPIRKLRHELSLCRVYVVSGTRRSFDRRPFGTGSTEKVRTEASRGASTSRNKGPTNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D3X2</accession>
    <name>A0AAD5D3X2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Signal recognition particle 19 kDa protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">M8C21_010917</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">AA19_3_7</strain>
            <tissue evidence="13">Leaf</tissue>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Component of the signal recognition particle (SRP) complex, a ribonucleoprotein complex that mediates the cotranslational targeting of secretory and membrane proteins to the endoplasmic reticulum (ER). Binds directly to 7SL RNA. Mediates binding of SRP54 to the SRP complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of a signal recognition particle complex that consists of a 7SL RNA molecule of 300 nucleotides and six protein subunits: SRP72, SRP68, SRP54, SRP19, SRP14 and SRP9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SRP19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005600">
        <property type="protein sequence ID" value="KAI7752837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D3X2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005786">
        <property type="term" value="C:signal recognition particle, endoplasmic reticulum targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006617">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane, signal sequence recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.56.30:FF:000002">
        <property type="entry name" value="Signal recognition particle 19kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.56.30">
        <property type="entry name" value="Signal recognition particle, SRP19-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002778">
        <property type="entry name" value="Signal_recog_particle_SRP19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036521">
        <property type="entry name" value="SRP19-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17453">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE 19 KD PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17453:SF0">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE 19 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01922">
        <property type="entry name" value="SRP19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69695">
        <property type="entry name" value="SRP19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0733">Signal recognition particle</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="109"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="116"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00033761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00033772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00045518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="KAI7752837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="136" mass="14939" checksum="4B9732A457E45826" modified="2024-05-29" version="1">MDGGTPNFKTWNIIYPVYINSKKTIAEGRRISATKACENPTALEIGDCCGHLKIPFAIEIDKAYPRDYMQVGRVRVLLKRPDGSPSNPLITSKKQLMIRIAELVPRHPGRLKKQEAAASASNAAPSKPGKGGKKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D4V5</accession>
    <name>A0AAD5D4V5_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dilute domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_012812</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005829">
        <property type="protein sequence ID" value="KAI7751730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D4V5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002710">
        <property type="entry name" value="Dilute_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052072">
        <property type="entry name" value="Vascular_dev_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16027:SF6">
        <property type="entry name" value="DILUTE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16027">
        <property type="entry name" value="DILUTE DOMAIN-CONTAINING PROTEIN YPR089W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51126">
        <property type="entry name" value="DILUTE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dilute" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7751730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="101" mass="11364" checksum="68D0782E580E914F" modified="2024-05-29" version="1" fragment="single">VLNIQQLYRISTMYYDDRNNTDSLSPEVIAKMRELMTEDSNNAVSGSFLLDDDPSLKSYIIPLSVDDLSKSMDQMINITDIALPPLVLHKLGLDFLLSPES</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D5K2</accession>
    <name>A0AAD5D5K2_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mechanosensitive ion channel MscS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_020398</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MscS (TC 1.A.23) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010005745">
        <property type="protein sequence ID" value="KAI7752150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D5K2"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008381">
        <property type="term" value="F:mechanosensitive monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006820">
        <property type="term" value="P:monoatomic anion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016688">
        <property type="entry name" value="MscS-like_plants/fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006685">
        <property type="entry name" value="MscS_channel_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31618:SF1">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31618">
        <property type="entry name" value="MECHANOSENSITIVE ION CHANNEL PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00924">
        <property type="entry name" value="MS_channel_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mechanosensitive ion channel MscS" evidence="3">
        <location>
            <begin position="2"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7752150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="152" mass="17639" checksum="1A2C107A1B9C180C" modified="2024-05-29" version="1">MIVDEMSILTTVFMRHDNQKVIYPNSVLATKPIGNFSRSPAMGDEIAFSISISTPENKIKEMKKKIKEYVGKKSDFWFDDPTIVVIGVEDMNTLNMVVWPNHVVNHHDMSRRWERRSELILEMVKVFRDLDIDYTMPPLNVNVNHIGGMPKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D745</accession>
    <name>A0AAD5D745_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL6 alpha-beta domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_016676</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000976">
        <property type="protein sequence ID" value="KAI7755603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D745"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.930.12:FF:000003">
        <property type="entry name" value="60S ribosomal protein L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.930.12:FF:000004">
        <property type="entry name" value="60S ribosomal protein L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.930.12">
        <property type="entry name" value="Ribosomal protein L6, alpha-beta domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000702">
        <property type="entry name" value="Ribosomal_uL6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036789">
        <property type="entry name" value="Ribosomal_uL6-like_a/b-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020040">
        <property type="entry name" value="Ribosomal_uL6_a/b-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002359">
        <property type="entry name" value="Ribosomal_uL6_CS2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11655:SF16">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11655">
        <property type="entry name" value="60S/50S RIBOSOMAL PROTEIN L6/L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00347">
        <property type="entry name" value="Ribosomal_L6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002162">
        <property type="entry name" value="Ribosomal_L6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56053">
        <property type="entry name" value="Ribosomal protein L6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00700">
        <property type="entry name" value="RIBOSOMAL_L6_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL6 alpha-beta" evidence="4">
        <location>
            <begin position="12"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL6 alpha-beta" evidence="4">
        <location>
            <begin position="102"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7755603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="195" mass="22184" checksum="B87748338BC179A8" modified="2024-05-29" version="1">MKTILSSETMDIPDEIEIKIKAKIIEVKGPRGTLTRNFKHLNLDFMLITDEETGKRKLKVDAWFGSRKTTASIRTALSHVSNLIIGVTQGFRYKMRFVYAHFPINASITNNNTAIEIRNFLGEKKVRKVDMLEGVTVVRSEKVKDELVLDGNDIELVSRSCALINQKCHVKNKDIRKFLDGIYVSDKAKIEEGDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5D8V8</accession>
    <name>A0AAD5D8V8_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">glutamate carboxypeptidase II</fullName>
            <ecNumber evidence="9">3.4.17.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_008739</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>Release of an unsubstituted, C-terminal glutamyl residue, typically from Ac-Asp-Glu or folylpoly-gamma-glutamates.</text>
            <dbReference type="EC" id="3.4.17.21"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Endomembrane system</location>
            <topology evidence="8">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M28 family. M28B subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.17.21" evidence="9"/>
    <dbReference type="EMBL" id="JAMZMK010001688">
        <property type="protein sequence ID" value="KAI7755190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5D8V8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd08022">
        <property type="entry name" value="M28_PSMA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000164">
        <property type="entry name" value="Os01g0740650 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.40">
        <property type="entry name" value="Transferrin receptor-like, dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007484">
        <property type="entry name" value="Peptidase_M28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039373">
        <property type="entry name" value="Peptidase_M28B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007365">
        <property type="entry name" value="TFR-like_dimer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036757">
        <property type="entry name" value="TFR-like_dimer_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10404">
        <property type="entry name" value="N-ACETYLATED-ALPHA-LINKED ACIDIC DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10404:SF46">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04389">
        <property type="entry name" value="Peptidase_M28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04253">
        <property type="entry name" value="TFR_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47672">
        <property type="entry name" value="Transferrin receptor-like dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Peptidase M28" evidence="11">
        <location>
            <begin position="80"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="Transferrin receptor-like dimerisation" evidence="10">
        <location>
            <begin position="352"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00052003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00060399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00066561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF04389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7755190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="411" mass="45561" checksum="A642C873D7742AC1" modified="2024-05-29" version="1">MEGCERLSEDEVERAGDVPLIPSLPISGADGEEILRWIGGVVAEDEWQGDVDAPVYRIGPGPGIIKLSYSAKQVISTIQNVIGIIEGTEEPDRFVILGNHRDAWTFGAVDPNSGTAALLEVAERFEKLRKEGWKPRRTIIFCNWDAEEYGLIGSTEWVEENREMLASKVVAYLNVDIAVAGAGFQASATPQLDQLIIEVTKQVEDPDNSSQTVYDAWVKTTKYPKIGRLGGGGSDYAAFVQHIGVPSTDISFGNGYPVYHSMYDDFVWMSKFGDPMFRRHVAAASIWGLVALQLADEEILPFNYSSYVNELQKSAEDLETELSDKSISLIPLFKSIEKMKNAAVRINDEVKIYAPSKHNDYGSKCFPGIDDAVENANKLDTKDSWHSVQHEIWRVSRAITQASLVLHGELT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DAE6</accession>
    <name>A0AAD5DAE6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">X8 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_008763</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000578">
        <property type="protein sequence ID" value="KAI7756107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DAE6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009506">
        <property type="term" value="C:plasmodesma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012946">
        <property type="entry name" value="X8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044788">
        <property type="entry name" value="X8_dom_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31044">
        <property type="entry name" value="BETA-1,3 GLUCANASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31044:SF71">
        <property type="entry name" value="MAJOR POLLEN ALLERGEN OLE E 10-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07983">
        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00768">
        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5042220286" description="X8 domain-containing protein" evidence="2">
        <location>
            <begin position="28"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="X8" evidence="3">
        <location>
            <begin position="30"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7756107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="114" mass="12399" checksum="64254E2C542F6D59" modified="2024-05-29" version="1">MAGKLSAYVSLFLLSFLSARTLTLVHAQKTWCVAKPSSSQAILLENINFACSQVDCGRLQKGGPCYSPDNPINHASIAMNLYFQSKGRNTWNCDFKNSGLLTVGDPSYGGCQYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DAZ8</accession>
    <name>A0AAD5DAZ8_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">M8C21_009195</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">AA19_3_7</strain>
            <tissue evidence="5">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000497">
        <property type="protein sequence ID" value="KAI7756247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DAZ8"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016125">
        <property type="term" value="P:sterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11071">
        <property type="entry name" value="CYP74"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000024">
        <property type="entry name" value="Allene oxide synthase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24286:SF302">
        <property type="entry name" value="ALLENE OXIDE SYNTHASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24286">
        <property type="entry name" value="CYTOCHROME P450 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0349">Heme</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="KAI7756247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="481" mass="54188" checksum="7D05FA4BF07AEE4B" modified="2024-05-29" version="1">MPSSPSSDHLATSSKLSLREIPGTYGTAFFGPIKDRYDYFYNQGKDEFFRKKIQKYNSTVFRTNMPPGPFISSDPRVVAVLDSKSFTILFDNSKVEKKNILDGTYMPSLSFFGGYRVCAFLDTDESNHHTLKSFFLTFLANSHKKFIPYMRTGLTEMFENIETEITDKKSSDFNVHSDNMAFGFVFRLVTGTNPLETKVKSKGPATIATWLALQLAPLGTSGLKFIPNFIDDIIHTVRLPFFVIKSGYKKLYNAVYDSATTLLDEAEKSGINREEACHNLVFLAAFNAFGGMKVLFPSLMKWISSAGESLHSRLAEEIRSVVKTEGDVSFSTLEKLPLTKSTVYEVLRIDPPVPFQYATAREDIIIESHDSAFEIKKGETIFGYQPFATKDPKVFNNPEEFVADRFVGEGEELLKYVYWSNARETENPSADNKQCPAKDLVVLCCRLMLVEFFLRYDTFTADTEVSGFGASVKITSLTKCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DBE7</accession>
    <name>A0AAD5DBE7_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">alcohol dehydrogenase</fullName>
            <ecNumber evidence="3">1.1.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">M8C21_000011</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">AA19_3_7</strain>
            <tissue evidence="11">Leaf</tissue>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a primary alcohol + NAD(+) = an aldehyde + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10736"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15734"/>
            <dbReference type="ChEBI" id="CHEBI:17478"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a secondary alcohol + NAD(+) = a ketone + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10740"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17087"/>
            <dbReference type="ChEBI" id="CHEBI:35681"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.1" evidence="3"/>
    <dbReference type="EMBL" id="JAMZMK010000954">
        <property type="protein sequence ID" value="KAI7755646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DBE7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004022">
        <property type="term" value="F:alcohol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051903">
        <property type="term" value="F:S-(hydroxymethyl)glutathione dehydrogenase [NAD(P)+] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046294">
        <property type="term" value="P:formaldehyde catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43880">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43880:SF40">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="KAI7755646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="269" mass="30735" checksum="5F37857AE0ABC7D8" modified="2024-05-29" version="1" fragment="single">MRVRNALNETHNRKKAKLSSDESLRAEVNIGRETIDLDDCFGELKEPRSFGPINIFATSQGVKGKQSNLNNVVRKEQVVRVKEYICKWAYECSIPFHAFEKGSYKSMAEAIAQFDRVKRGLGATLNVENQKRLLGDVFSLGAAGLANHWYMNYLGLSNKFELGNTKKKFGVTKLVNPKYDKKPVQEVIVELTSRRLDRSVECTGHIDARDWDIVLVGFPHKDAMFKTSPMNLLNERTLKHTFFGLEKFIKHEIYNTFDLLLQGEGLRCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DEG1</accession>
    <name>A0AAD5DEG1_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Luc7-like protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_018741</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Luc7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000016">
        <property type="protein sequence ID" value="KAI7758219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DEG1"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006376">
        <property type="term" value="P:mRNA splice site recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004882">
        <property type="entry name" value="Luc7-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12375">
        <property type="entry name" value="RNA-BINDING PROTEIN LUC7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03194">
        <property type="entry name" value="LUC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7758219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="179" mass="20446" checksum="3A02616C91B4DBA0" modified="2024-05-29" version="1">MDAQRALLDELMGSARNLTEEERRGHKEITWDDKEVCGFYMVKFCPHDLFVNTRSDLGPCPKIHDPKLKESFEASPRHDSYAPRFEADLAHFCEKLMDLDRKVRRGRERLAQEVELPPPPPLPVEKSEQLSVLEEKIKNLLESVEALGEAGKVDEAEALMRKVLAVGFLKLQVMVFSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DF29</accession>
    <name>A0AAD5DF29_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">NAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_029122</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000107">
        <property type="protein sequence ID" value="KAI7757521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DF29"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.150.80:FF:000001">
        <property type="entry name" value="NAC domain-containing protein 73"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.80">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003441">
        <property type="entry name" value="NAC-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036093">
        <property type="entry name" value="NAC_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044799">
        <property type="entry name" value="SOG1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31079">
        <property type="entry name" value="NAC DOMAIN-CONTAINING PROTEIN 73"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31079:SF46">
        <property type="entry name" value="NAC DOMAIN-CONTAINING PROTEIN 73"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02365">
        <property type="entry name" value="NAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101941">
        <property type="entry name" value="NAC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51005">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NAC" evidence="7">
        <location>
            <begin position="49"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="KAI7757521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="275" mass="31513" checksum="7B12BEAABDBAAFFA" modified="2024-05-29" version="1">MTWCNDCSVASDVIMRKVPSSNDKTMSCPSCGHQINRYEKARIHDLPGLPAGVKFDPSDQEILEHLEAKVRLDAHKLHPLIDEFIPTLEGENGICYTHPEKLPGVSQDGLVRHFFHRPSKAYTTGTRKRRKVHSEVDGSETRWHKTGKTRPVLSKTKVKGYKKILVLYTNYGKQRKPEKTNWIMHQYHLGNNEDEKEGELVVSKVFYQTQPRQCGSNIVKDNNKSTIVKRNCLNATFVEYFSTTPVSFQHNPQHTHDLNLRDESTVGSMNICQQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5DI56</accession>
    <name>A0AAD5DI56_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Secoisolariciresinol dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">M8C21_005427</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">AA19_3_7</strain>
            <tissue evidence="3">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010000035">
        <property type="protein sequence ID" value="KAI7758015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5DI56"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43180">
        <property type="entry name" value="3-OXOACYL-(ACYL-CARRIER-PROTEIN) REDUCTASE (AFU_ORTHOLOGUE AFUA_6G11210)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43180:SF30">
        <property type="entry name" value="MOMILACTONE A SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="KAI7758015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="274" mass="28923" checksum="559782336A229E02" modified="2024-05-29" version="1">MADAMIAPQPIPRLAGKVAIITGGARGIGETTARLFVKHGAKVVIADILDDLGQTVCESIGVEMASFVHCDVSIESDVERVVNFTMDKHGKLDIMINNAAIAGALKFSILDNEVTDFERVMSVNVTGVFLGTKHAARAMIPARCGSIVTIGSIASSVGGNTSHAYASSKHAIVGLTKNVAAELGEYGIRLNCVSPHLVVTPATVPIIEKYPDLYTDVYSNLKGAVLREEDVAEATLFLAGDESKYISGHNLALDGGFSVINPSFGLFSSRFRGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="4" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G4I7</accession>
    <name>A0AAD5G4I7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">M8C21_031716</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">AA19_3_7</strain>
            <tissue evidence="2">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011203">
        <property type="protein sequence ID" value="KAI7728485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G4I7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="KAI7728485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="173" mass="19022" checksum="C31A2CF1FBEAC878" modified="2024-05-29" version="1">MERRWCVDGRLGKEGGGSTMVVCRGREDQLSRRWRFVVLGCCLMPVAAVILAGQNIRSFDCVMIKGALKEISQPTPKTVALVDSSGVLFLILDMGLRIVEPKVSAPVGKKSRKLLSKYRPILKMEFGLPGKEEGIYGRSVVGEYPASLSFQALLTNCTNSLAPVHMLIVLKRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G5L5</accession>
    <name>A0AAD5G5L5_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_005779</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the MIP/aquaporin (TC 1.A.8) family. PIP (TC 1.A.8.11) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010011235">
        <property type="protein sequence ID" value="KAI7728323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G5L5"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015267">
        <property type="term" value="F:channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00333">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1080.10:FF:000001">
        <property type="entry name" value="Probable aquaporin PIP1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="entry name" value="Glycerol uptake facilitator protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034294">
        <property type="entry name" value="Aquaporin_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000425">
        <property type="entry name" value="MIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022357">
        <property type="entry name" value="MIP_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00861">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45687">
        <property type="entry name" value="AQUAPORIN OR AQUAGLYCEROPORIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00230">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00783">
        <property type="entry name" value="MINTRINSICP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81338">
        <property type="entry name" value="Aquaporin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00221">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="81"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="124"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="169"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="199"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="248"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7728323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="284" mass="30413" checksum="059ECEA3908BD790" modified="2024-05-29" version="1">MGKDVEVGGHEYGAKDYQDPPPADLFDAEELTKWSFYRAIIAEFIATLLFLYITVLTVIGYKSQTDPDHSTDQCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVTLPRAFMYIVAQCLGAVCGCGLVKAFQKTYYNTYGGGANELADGYSKGTGLGAEIIGTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVMYGKDKAWDDQWLFWVGPMIGAAIAAFYHQYVLRAGAVKALGSFRSNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G7S9</accession>
    <name>A0AAD5G7S9_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">M8C21_002995</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">AA19_3_7</strain>
            <tissue evidence="9">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010264">
        <property type="protein sequence ID" value="KAI7732280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G7S9"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00265">
        <property type="entry name" value="MADS_MEF2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1810.10">
        <property type="entry name" value="Transcription factor, MADS-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050142">
        <property type="entry name" value="MADS-box/MEF2_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033896">
        <property type="entry name" value="MEF2-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002487">
        <property type="entry name" value="TF_Kbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002100">
        <property type="entry name" value="TF_MADSbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036879">
        <property type="entry name" value="TF_MADSbox_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48019">
        <property type="entry name" value="SERUM RESPONSE FACTOR HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01486">
        <property type="entry name" value="K-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00319">
        <property type="entry name" value="SRF-TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00404">
        <property type="entry name" value="MADSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00432">
        <property type="entry name" value="MADS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55455">
        <property type="entry name" value="SRF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51297">
        <property type="entry name" value="K_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00350">
        <property type="entry name" value="MADS_BOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50066">
        <property type="entry name" value="MADS_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MADS-box" evidence="7">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="K-box" evidence="8">
        <location>
            <begin position="90"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="90"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="KAI7732280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="202" mass="23779" checksum="4DC654DEF95438EB" modified="2024-05-29" version="1">MGRGKIEIKRIENTSNRQVTYSKRKNGIIKKAKEITVLCDANVSLVIYGSSGKMYEYCSPKTNLIDMLDRYQRLSGNKLWDAKHEAGFSLFNLQNEIDRIKKENESMQIELRHLKGEDITSLNYEELIGYEDALENGLTNIREKKDEIPKIMRKREQVLEEENKHLMYMVQQTEMAAMGDYQQHEPFSFRVQPMQPNLHERM</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="5" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G8H7</accession>
    <name>A0AAD5G8H7_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_025785</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010246">
        <property type="protein sequence ID" value="KAI7732387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G8H7"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025886">
        <property type="entry name" value="PP2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32278">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32278:SF135">
        <property type="entry name" value="F-BOX PROTEIN PP2-B12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14299">
        <property type="entry name" value="PP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7732387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="189" mass="21696" checksum="4CCCDA7788722C31" modified="2024-05-29" version="1">MAAAAPRLLYGSQEELKELISKGVLLNMGKTWFSLNKNGEHCEMISAAECLIPIASIYYNDPYSSDYNSRFAMGSYLAYNRKFKIRVRTQFLSPKTVYEVNLVFKFIKKNPSDEPPYIALEYKLAGNTKFSIVHLADEREDGWLMARLYELTSESRNVDLEIIFKSSKDYYSPVLVVDGIEFQPLEKVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="8" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5G9Q6</accession>
    <name>A0AAD5G9Q6_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">HhH-GPD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">M8C21_001329</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">AA19_3_7</strain>
            <tissue evidence="12">Leaf</tissue>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DNA glycosylase family. DEMETER subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010010257">
        <property type="protein sequence ID" value="KAI7732343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5G9Q6"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035514">
        <property type="term" value="F:DNA demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019104">
        <property type="term" value="F:DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003906">
        <property type="term" value="F:DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141166">
        <property type="term" value="P:chromosomal 5-methylcytosine DNA demethylation pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1670.10:FF:000004">
        <property type="entry name" value="DNA glycosylase/AP lyase ROS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.10">
        <property type="entry name" value="Helix-hairpin-Helix base-excision DNA repair enzymes (C-terminal)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.30">
        <property type="entry name" value="Hypothetical protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044811">
        <property type="entry name" value="DME/ROS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003651">
        <property type="entry name" value="Endonuclease3_FeS-loop_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023170">
        <property type="entry name" value="HhH_base_excis_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46213:SF13">
        <property type="entry name" value="DEMETER-LIKE PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46213">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR DEMETER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00525">
        <property type="entry name" value="FES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="DNA-glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HhH-GPD" evidence="11">
        <location>
            <begin position="317"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="260"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="260"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="282"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="KAI7732343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="679" mass="76702" checksum="819C8CBAC05CDF3C" modified="2024-05-29" version="1" fragment="single">MKITKPRPKVDLDDETSRVWRLLLEDINSEGIDGTDEDNTKWWEEERTVFSGRAASFIARMHLVQGDRRFSPWKGSVVDSVIGVFLTQNVSDHLSSSAFMNLAARYPLPPKSSIEALEEEKSSMSVKEPFELDVEKCHDDNLNYQLSALYEEKEVEDLVFSHSSVDTCSNTAQSSISDTAERLGSGLVSNSLTNVNNSGEHIDMTEERTIMPNKHEDKCASEQSEILAESKIATNQETEISDCQAVSENSMSVINQQKEISDVTESNSNEDGNFKVSASRSSKVKKVSASRSSKVKKGKNGKAKKIKPDWDSMRKTAQIKNKRERSPNAMDSLDYEAVRRADVNELSDVIKDRGMNNMLAQRIKAFLNRLVRDHGSIDLEWLRDVPPDKAKEYLLSFKGLGLKSVECVRLLTLHHLAFPVDTNVGRIAVRLGWVPLQPLPESLQLHLLEMYPILETIQKYLWPRLCKLDQRTLYELHYQMITFGKVFCTKNKPNCNSCPLRAECRHFASAFASSRLALPAPEEKRIVTAVTENISHGQSQSFTGIINQHKLTLPPADPYLQRQYEDQNLDPVIEVPATPEPIIEQPATPEPEKIQEEFDIEDFCEEIPIIRLNMEAFTQNVQNYVQNNMEVAEGDMSRALVALTSEAASIPTPKLKNVSQLRTEHQVYELPDSHPLLEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="3" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GAW0</accession>
    <name>A0AAD5GAW0_AMBAR</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">M8C21_032128</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">AA19_3_7</strain>
            <tissue evidence="1">Leaf</tissue>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009661">
        <property type="protein sequence ID" value="KAI7734709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GAW0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="KAI7734709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <sequence length="78" mass="9213" checksum="5FE4BD921A9E0997" modified="2024-05-29" version="1" fragment="single">DADLLNNTRNRSSKEASRYQRTRNHILLIKPYASDPNASHSFNNILSRFLHRFDIQVWDFVFLSNLKLWCLGFSNKSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="6" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GCB0</accession>
    <name>A0AAD5GCB0_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein root UVB sensitive 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">M8C21_021711</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">AA19_3_7</strain>
            <tissue evidence="4">Leaf</tissue>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RUS1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009624">
        <property type="protein sequence ID" value="KAI7734908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GCB0"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006968">
        <property type="entry name" value="RUS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055412">
        <property type="entry name" value="UVB_sens_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054549">
        <property type="entry name" value="UVB_sens_RUS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12770:SF20">
        <property type="entry name" value="PROTEIN ROOT UVB SENSITIVE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12770">
        <property type="entry name" value="RUS1 FAMILY PROTEIN C16ORF58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24160">
        <property type="entry name" value="UVB_sens_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04884">
        <property type="entry name" value="UVB_sens_prot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein root UVB sensitive/RUS" evidence="2">
        <location>
            <begin position="8"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="Root UVB sensitive protein C-terminal" evidence="3">
        <location>
            <begin position="241"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="KAI7734908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP001206925"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="403" mass="44368" checksum="EC01ED072F61AADD" modified="2024-05-29" version="1">MCVHICENELMSFIRSYVVPEGFPDSVSPSYVPYMTWRALKHFFGGAMGVFTTQALLHSVGVSKSQAMPGAVAINWIIKDGSGRIGKMLFSRQGKKFDYDLKQLRLSGDLLMELGAAVELATTAVPHLFLPLACAANVAKNVAAVTSTSTRTPIYKAFAKGENIGDVTAKGECVGNVADLLGTGFSIIMAKRNPSLFTTFALLSCGYLFSSYREVKSVVLHTLNRARFTVAVESFLKTGSVPTLQEGNMMETVFNIPWCKDGPVVLGSRFKEAFQDANSFYDVKPVFEKERYVVAYNPLKGNIYAVLKDKAKSDDILKAAFHAHVLLYVLRSAKHIQVSNEYTSGDHVTPLPRSVDLQAHVAESYNMVSALYEPFKSKAKEQGWVMSDSLLNPGRARICEQVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2024-05-29" modified="2026-01-28" version="7" xmlns="https://uniprot.org/uniprot">
    <accession>A0AAD5GCV3</accession>
    <name>A0AAD5GCV3_AMBAR</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA polymerase alpha subunit B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">M8C21_017866</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Ambrosia artemisiifolia</name>
        <name type="common">Common ragweed</name>
        <dbReference type="NCBI Taxonomy" id="4212"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Viridiplantae</taxon>
            <taxon>Streptophyta</taxon>
            <taxon>Embryophyta</taxon>
            <taxon>Tracheophyta</taxon>
            <taxon>Spermatophyta</taxon>
            <taxon>Magnoliopsida</taxon>
            <taxon>eudicotyledons</taxon>
            <taxon>Gunneridae</taxon>
            <taxon>Pentapetalae</taxon>
            <taxon>asterids</taxon>
            <taxon>campanulids</taxon>
            <taxon>Asterales</taxon>
            <taxon>Asteraceae</taxon>
            <taxon>Asteroideae</taxon>
            <taxon>Heliantheae alliance</taxon>
            <taxon>Heliantheae</taxon>
            <taxon>Ambrosia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2022-06" db="EMBL/GenBank/DDBJ databases">
            <title>Uncovering the hologenomic basis of an extraordinary plant invasion.</title>
            <authorList>
                <person name="Bieker V.C."/>
                <person name="Martin M.D."/>
                <person name="Gilbert T."/>
                <person name="Hodgins K."/>
                <person name="Battlay P."/>
                <person name="Petersen B."/>
                <person name="Wilson J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">AA19_3_7</strain>
            <tissue evidence="8">Leaf</tissue>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA polymerase alpha subunit B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="JAMZMK010009476">
        <property type="protein sequence ID" value="KAI7735508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0AAD5GCV3"/>
    <dbReference type="Proteomes" id="UP001206925">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005658">
        <property type="term" value="C:alpha DNA polymerase:primase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.60:FF:000004">
        <property type="entry name" value="DNA polymerase alpha subunit B"/>
        <property type="m
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